Eras cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eras
Entrez Gene ID	353283
Full Name	ES cell-expressed Ras
Synonyms	HRAS2,HRasp,Ha-Ras2,ecat5
General protein information	Preferred Names ES cell-expressed Ras Names GTPase ERas E-Ras embryonic stem cell-expressed Ras small GTPase E-Ras
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X A1.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_181548.2	NP_853526.1	GTPase ERas precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ERAS	XP_002700122.2
Mus musculus (house mouse)	Eras	NP_853526.1
Homo sapiens (human)	ERAS	NP_853510.1
Rattus norvegicus (Norway rat)	Eras	NP_001102845.1
Rattus norvegicus (Norway rat)	LOC100911243	XP_003752059.2
	ERAS	XP_001101805.1
Canis lupus familiaris (dog)	ERAS	XP_548988.1

Gene Ontology
Bibliography

Related articles in PubMed

The roles of ERAS during cell lineage specification of mouse early embryonic development.
Zhao ZA, Yu Y, Ma HX, Wang XX, Lu X, Zhai Y, Zhang X, Wang H, Li L
Open biology5(8)(2015 Aug)

Stimulation of somatic cell reprogramming by ERas-Akt-FoxO1 signaling axis.
Yu Y, Liang D, Tian Q, Chen X, Jiang B, Chou BK, Hu P, Cheng L, Gao P, Li J, Wang G
Stem cells (Dayton, Ohio)32(2)349-63(2014 Feb)

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
Tang F, Barbacioru C, Nordman E, Bao S, Lee C, Wang X, Tuch BB, Heard E, Lao K, Surani MA
PloS one6(6)e21208(2011)

The DM domain protein DMRT1 is a dose-sensitive regulator of fetal germ cell proliferation and pluripotency.
Krentz AD, Murphy MW, Kim S, Cook MS, Capel B, Zhu R, Matin A, Sarver AL, Parker KL, Griswold MD, Looijenga LH, Bardwell VJ, Zarkower D
Proceedings of the National Academy of Sciences of the United States of America106(52)22323-8(2009 Dec)

Interaction of galectin-1 with caveolae induces mouse embryonic stem cell proliferation through the Src, ERas, Akt and mTOR signaling pathways.
Lee MY, Lee SH, Park JH, Han HJ
Cellular and molecular life sciences : CMLS66(8)1467-78(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ERAS may also play important roles in cell lineage specification during mouse early embryonic development.
Title: The roles of ERAS during cell lineage specification of mouse early embryonic development.

Collectively, these results unravel the significance of the ERas-Akt-FoxO1 signaling axis in iPSC generation, suggesting a possibly shared molecular basis for both somatic reprogramming and cancer initiation
Title: Stimulation of somatic cell reprogramming by ERas-Akt-FoxO1 signaling axis.

galectin-1 increased DNA synthesis in mouse ES cells via Src, caveolin-1 Akt, and mTOR signaling pathways
Title: Interaction of galectin-1 with caveolae induces mouse embryonic stem cell proliferation through the Src, ERas, Akt and mTOR signaling pathways.

rasH2 may have a role in susceptibility to chemically-induced tongue and esophageal neoplasms
Title: A novel rasH2 mouse carcinogenesis model that is highly susceptible to 4-NQO-induced tongue and esophageal carcinogenesis is useful for preclinical chemoprevention studies.

ERas and Rheb are two Ras-related proteins involved in the phosphatidylinositol 3-kinase/mTOR pathway; ERas is localized at the plasma membrane, while Rheb is on endomembranes.
Title: Differential membrane localization of ERas and Rheb, two Ras-related proteins involved in the phosphatidylinositol 3-kinase/mTOR pathway.

The following Eras gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eras cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08084	NM_181548.2 Latest version!	Mus musculus ES cell-expressed Ras (Eras), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eras ( NM_181548.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_853526.1 Mus musculus ES cell-expressed Ras (Eras), mRNA.

Price & Availability↑

CloneID	OMu08084
Clone ID Related Accession (Same CDS sequence)	NM_181548.2
Accession Version	NM_181548.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	GTPase ERas precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB093573.1. On Jun 11, 2003 this sequence version replaced NM_181548.1. ##Evidence-Data-START## Transcript exon combination :: AB093573.1, BC119277.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTTGC CTACAAAGTC TAGCATCTTG GACCTGAGCT CCGGCACCCC ATGCACCAGA 
TCTCCAGAGG AAAGTCACGA GGCTTGGGCA CAGTGCAAAG ATGCTGGCAG GCAGCTACCC 
GAGTACAAGG CAGTGGTGGT GGGTGCAAGT GGTGTTGGTA AAAGTGCTCT CACCATCCAG 
ATGACTCACC AATGCTTCGT GAAAGACCAT GACCCCACTA TCCAAGATTC CTACTGGAAG 
GAAGTGGCCA GGGACAACGG AGGCTACATT CTAAATGTTC TGGATACATC TGGGCAGGAT 
ATTCACCGGG CTCTGCGTGA CCAGTGCTTG GCATCTGGTG ATGGTGTGCT GGGCGTCTTT 
GCTCTTGACG ACCCCTCGTC TCTGGACCAG TTGCAGCAGA TATGGTCCAC CTGGACCCCT 
CACCACAAGC AGCCTCTGGT ACTAGTGGGC AACAAGTGTG ACCTGGTGAC CACTGCTGGA 
GATGCTCATG CTGCCGCAGC CCTCCTTGCT CACAAGTTGG GGGCCCCCTT GGTGAAGACC 
TCAGCCAAGA CGCGGCAAGG TGTGGAGGAA GCCTTTGCCC TGCTTGTCCA TGAGATTCAG 
AGGGCCCAGG AGGCTGTGGC CGAATCAAGC AAGAAGACCC GACACCAGAA AGCCGTGTGT 
AGCTGTGGCT GCTCTGTAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_853526.1
CDS	178..861
Translation	MALPTKSSILDLSSGTPCTRSPEESHEAWAQCKDAGRQLPEYKAVVVGASGVGKSALTIQMTHQCFVKDHDPTIQDSYWKEVARDNGGYILNVLDTSGQDIHRALRDQCLASGDGVLGVFALDDPSSLDQLQQIWSTWTPHHKQPLVLVGNKCDLVTTAGDAHAAAALLAHKLGAPLVKTSAKTRQGVEEAFALLVHEIQRAQEAVAESSKKTRHQKAVCSCGCSVA

Target ORF information:

RefSeq Version	NM_181548.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ES cell-expressed Ras (Eras), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181548.2

ORF Insert Sequence:

ATGGCTTTGC CTACAAAGTC TAGCATCTTG GACCTGAGCT CCGGCACCCC ATGCACCAGA 
TCTCCAGAGG AAAGTCACGA GGCTTGGGCA CAGTGCAAAG ATGCTGGCAG GCAGCTACCC 
GAGTACAAGG CAGTGGTGGT GGGTGCAAGT GGTGTTGGTA AAAGTGCTCT CACCATCCAG 
ATGACTCACC AATGCTTCGT GAAAGACCAT GACCCCACTA TCCAAGATTC CTACTGGAAG 
GAAGTGGCCA GGGACAACGG AGGCTACATT CTAAATGTTC TGGATACATC TGGGCAGGAT 
ATTCACCGGG CTCTGCGTGA CCAGTGCTTG GCATCTGGTG ATGGTGTGCT GGGCGTCTTT 
GCTCTTGACG ACCCCTCGTC TCTGGACCAG TTGCAGCAGA TATGGTCCAC CTGGACCCCT 
CACCACAAGC AGCCTCTGGT ACTAGTGGGC AACAAGTGTG ACCTGGTGAC CACTGCTGGA 
GATGCTCATG CTGCCGCAGC CCTCCTTGCT CACAAGTTGG GGGCCCCCTT GGTGAAGACC 
TCAGCCAAGA CGCGGCAAGG TGTGGAGGAA GCCTTTGCCC TGCTTGTCCA TGAGATTCAG 
AGGGCCCAGG AGGCTGTGGC CGAATCAAGC AAGAAGACCC GACACCAGAA AGCCGTGTGT 
AGCTGTGGCT GCTCTGTAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The roles of ERAS during cell lineage specification of mouse early embryonic development.
Open biology5(8)(2015 Aug)
Zhao ZA,Yu Y,Ma HX,Wang XX,Lu X,Zhai Y,Zhang X,Wang H,Li L

Stimulation of somatic cell reprogramming by ERas-Akt-FoxO1 signaling axis.
Stem cells (Dayton, Ohio)32(2)349-63(2014 Feb)
Yu Y,Liang D,Tian Q,Chen X,Jiang B,Chou BK,Hu P,Cheng L,Gao P,Li J,Wang G

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
PloS one6(6)e21208(2011)
Tang F,Barbacioru C,Nordman E,Bao S,Lee C,Wang X,Tuch BB,Heard E,Lao K,Surani MA

The DM domain protein DMRT1 is a dose-sensitive regulator of fetal germ cell proliferation and pluripotency.
Proceedings of the National Academy of Sciences of the United States of America106(52)22323-8(2009 Dec)
Krentz AD,Murphy MW,Kim S,Cook MS,Capel B,Zhu R,Matin A,Sarver AL,Parker KL,Griswold MD,Looijenga LH,Bardwell VJ,Zarkower D

Interaction of galectin-1 with caveolae induces mouse embryonic stem cell proliferation through the Src, ERas, Akt and mTOR signaling pathways.
Cellular and molecular life sciences : CMLS66(8)1467-78(2009 Apr)
Lee MY,Lee SH,Park JH,Han HJ