Cngb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cngb1
Entrez Gene ID	333329
Full Name	cyclic nucleotide gated channel beta 1
Synonyms	Cngb1b,Garp2,Gm1959
General protein information	Preferred Names cyclic nucleotide gated channel beta 1 Names cyclic nucleotide-gated cation channel beta-1 cyclic nucleotide-gated channel beta subunit 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001195413.1	NP_001182342.1	cyclic nucleotide-gated cation channel beta-1 isoform 1
XM_006531140.2	XP_006531203.1	cyclic nucleotide-gated cation channel beta-1 isoform X1
XM_006531140.3	XP_006531203.1	cyclic nucleotide-gated cation channel beta-1 isoform X1
NM_145601.1	NP_663576.1	cyclic nucleotide-gated cation channel beta-1 isoform 2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	cngb1a	XP_696619.6
Mus musculus (house mouse)	Cngb1	NP_001182342.1
Xenopus tropicalis (tropical clawed frog)	cngb1	XP_004914384.1

Gene Ontology
Bibliography

Related articles in PubMed

Patients and animal models of CNG?1-deficient retinitis pigmentosa support gene augmentation approach.
Petersen-Jones SM, Occelli LM, Winkler PA, Lee W, Sparrow JR, Tsukikawa M, Boye SL, Chiodo V, Capasso JE, Becirovic E, Sch?n C, Seeliger MW, Levin AV, Michalakis S, Hauswirth WW, Tsang SH
The Journal of clinical investigation128(1)190-206(2018 Jan)

Two pathways of rod photoreceptor cell death induced by elevated cGMP.
Wang T, Tsang SH, Chen J
Human molecular genetics26(12)2299-2306(2017 06)

GARP2 accelerates retinal degeneration in rod cGMP-gated cation channel ?-subunit knockout mice.
DeRamus ML, Stacks DA, Zhang Y, Huisingh CE, McGwin G, Pittler SJ
Scientific reports742545(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

olfactory sensory neurons in NCKX4(-/-);CNGB1(DeltaCaM) mice displayed markedly reduced electro-olfactogram amplitude accompanied by alterations in other response attributes
Title: Simultaneous Loss of NCKX4 and CNG Channel Desensitization Impairs Olfactory Sensitivity.

Eliminating Cngb1 and reducing RDS leads to additive defects in RDS expression levels and rod electroretinogram (ERG) function, (e.g., Cngb1-/-/rds+/- versus rds+/- or Cngb1-/-) but not to additive defects in rod ultrastructure.
Title: Role of RDS and Rhodopsin in Cngb1-Related Retinal Degeneration.

Peripherin-2 links CNGB1 to the light-detector rhodopsin in outer segments of rod photoreceptors.
Title: Peripherin-2 couples rhodopsin to the CNG channel in outer segments of rod photoreceptors.

Overexpression of rod photoreceptor glutamic acid rich protein 2 (GARP2) increases gain and slows recovery in mouse retina
Title: Overexpression of rod photoreceptor glutamic acid rich protein 2 (GARP2) increases gain and slows recovery in mouse retina.

These results confirm that rods are marginally functional in the absence of the beta-subunit and in addition show that CFF (critical flicker frequency)may be a more sensitive measure of remaining functional cone vision
Title: Age-related changes in Cngb1-X1 knockout mice: prolonged cone survival.

The following Cngb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cngb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu41571	XM_006531140.3 Latest version!	Mus musculus cyclic nucleotide gated channel beta 1 (Cngb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu11862	NM_145601.1 Latest version!	Mus musculus cyclic nucleotide gated channel beta 1 (Cngb1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OMu17438	NM_001195413.1 Latest version!	Mus musculus cyclic nucleotide gated channel beta 1 (Cngb1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$776.30 $1109.00
View Old Accession Versions >>
OMu41571	XM_006531140.2	Mus musculus cyclic nucleotide gated channel beta 1 (Cngb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cngb1 ( XM_006531140.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531203.1 Mus musculus cyclic nucleotide gated channel beta 1 (Cngb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu41571
Clone ID Related Accession (Same CDS sequence)	XM_006531140.2 , XM_006531140.3
Accession Version	XM_006531140.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cyclic nucleotide-gated cation channel beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006531140.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAAC CAGGCCTGAA CATGGAGCTG AATCGCCTGG TTCAGGACCA GCCACCTGGC 
CAGGAGGGCC CCAGACCTGG CCCCCCAAAC CCAGCCGAGC ACCTCCCCAA TGTGCCCAGC 
TATCGGCCCG CAACCACCCG CATCCCTGTC CTCGTTTCCC GGAGGACGGC CTTGTCCAAC 
TCCAACTTCA CCAAGGAGAT CAGGAGCTCC ATCCGTCGCC TGGTACCAGC CACAAAGGAG 
CACCCGGAGC TCCAGGTAGA AGACACGGAT GCTGATAGTG GCCCCCTCAT CCCAGAGGAG 
ACACTCCCTC CACCTGAGAG ACCACCACCG TCTCCTGTCA AGTCGGACAC CCTCACAGTT 
CCCGGCGCCG CTGCAGCAGG CCACAGGAAG AAGCTACCTT CTCAGGATGA TGAGGCCGAA 
GAGCTCAAGG CCCTGTCACC AGCTGAGTCC CCAGTGGTTG CCTGGTCGGA CCCCACCACC 
CCACAGGAGG CTGATGGCCA GGACCGAGCA GCCTCCACCG CCAGCCAGAA CAGTGCCATC 
ATCAATGACC GGCTCCAGGA GCTGGTGAAG ATGTTCAAGG AGCGGACGGA GAAGGTGAAG 
GAGAAGCTCA TTGACCCTGA CGTCACCTCT GATGAGGAGA GCCCCAAGCC CTCCCCGGCC 
AAGAAGGCCC CAGAACCAGA CCCAGCCCAG AAGCCAGCGG AGGCAGAGGT GGCAGAGGAG 
GAACACTACT GCGACATGCT CTGCTGCAAG TTCAAGCGCA GGCCCTTGAA GATGTACCGG 
TTCCCCCAGA GTATCGACCC ACTGACCAAC CTCATGTACA TCTTGTGGCT GTTCTTTGTG 
GTGCTGGCCT GGAACTGGAA CTGCTGGCTG ATCCCTGTGC GCTGGGCCTT CCCGTACCAG 
CGGGCAGACA ACATCCACTT CTGGCTCCTC ATGGATTACT TGTGCGACTT CATCTACCTC 
CTGGACATCA CCGTGTTCCA GATGCGCCTG CAGTTTGTCA AAGGCGGGGA CATCATTACA 
GACAAGAAGG AGATGCGTAA TAACTACCTG AAGTCTCGCC GGTTTAAGAT GGACCTGCTC 
TGCCTCCTGC CCTTGGACTT TCTCTACTTG AAACTTGGCA TCAACCCTCT CCTTCGCCTG 
CCCCGCTGCC TGAAGTACAT GGCCTTCTTT GAGTTTAATA ACCGCCTGGA AGCCATCCTC 
AGCAAAGCCT ACGTTTACAG AGTCATCAGG ACCACGGCCT ACCTGCTGTA CAGCCTGCAC 
CTCAACTCCT GCCTTTACTA CTGGGCATCA GCCTTCCAGG GCATCGGCTC CACTCACTGG 
GTTTACGACG GAGTGGGGAA CAGTTATATT CGATGCTACT ACTGGGCTGT GAAAACCCTC 
ATCACCATCG GAGGACTGCC CGACCCCCAG ACGCTCTTTG AGATTGTCTT CCAGCTGCTG 
AACTATTTTA CCGGGGTCTT TGCTTTCTCT GTGATGATTG GACAGATGAG AGATGTGGTG 
GGGGCCGCCA CAGCGGGGCA GACCTACTAC CGCAGCTGCA TGGACAGCAC AGTGAAGTAC 
ATGAACTTCT ACAAGATCCC CAGGTCTGTG CAGAACCGTG TCAAGACCTG GTATGAGTAC 
ACCTGGCATT CGCAAGGCAT GCTGGATGAG TCAGAGCTGA TGGTACAACT TCCGGACAAG 
ATGCGACTGG ACCTGGCCAT TGATGTGAAC TACAGTATTG TCAGCAAAGT GGCCCTCTTT 
CAGGGCTGTG ACCGGCAGAT GATCTTCGAC ATGCTCAAGC GGCTTCGTTC TGTTGTCTAC 
CTACCCAATG ACTATGTGTG CAAGAAGGGG GAGATTGGCC GAGAGATGTA CATTATCCAG 
GCGGGGCAGG TGCAGGTGCT GGGCGGCCCA GATGGGAAGG CTGTACTGGT GACACTCAAA 
GCCGGATCGG TGTTTGGAGA AATAAGCTTG CTGGCTGTCG GGGGTGGCAA CCGGCGCACA 
GCCAACGTGG TGGCCCATGG CTTCACCAAC CTCTTCATAC TGGACAAGAA GGACTTGAAT 
GAAATTTTAG TGCATTACCC GGAATCTCAG AAGTTACTTC GGAAGAAGGC CAGGCGCATG 
CTAAGAAATA ACAACAAACC CAAGGAGGAA AAGAGTGTGC TCATCCTGCC CCCGCGTGCG 
GGCACCCCGA AGCTCTTCAA TGCTGCCCTG GCTGCTGCAG GAAAGATGGG CCCCAGGGGA 
GCCAAGGGCG GCAAGCTCGC CCACCTGAGA GCCAGGCTCA AGGAACTGGC TGCACTGGAG 
GCAGCCGCGC GACAGCAACA GCTGCTGGAA CAGGCCAAGA GCTCGCAAGA AGCTGGGGGA 
GAGGAGGGCT CTGGGGCCAC AGACCAACCT GCACCCCAGG AGCCACCAGA GCCCAAGGAC 
CCTCCGAAGC CACCAGGGCC ACCAGAGCCC TCAGCCCAGA GCTCTCCACC TCCAGCCTCA 
GCAAAGCCCG AGGAAAGCAC GGGGGAGGCA GCAGGGCCCC CGGAGCCCTC GGTGAGGATC 
CGTGTGAGTC CAGGCCCTGA TCCCGGGGAG CAGACACTGT CGGTGGAGGT GCTGGAGGAG 
AAGAAAGAGG GGGCGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531203.1
CDS	307..2907
Translation	MAKPGLNMELNRLVQDQPPGQEGPRPGPPNPAEHLPNVPSYRPATTRIPVLVSRRTALSNSNFTKEIRSSIRRLVPATKEHPELQVEDTDADSGPLIPEETLPPPERPPPSPVKSDTLTVPGAAAAGHRKKLPSQDDEAEELKALSPAESPVVAWSDPTTPQEADGQDRAASTASQNSAIINDRLQELVKMFKERTEKVKEKLIDPDVTSDEESPKPSPAKKAPEPDPAQKPAEAEVAEEEHYCDMLCCKFKRRPLKMYRFPQSIDPLTNLMYILWLFFVVLAWNWNCWLIPVRWAFPYQRADNIHFWLLMDYLCDFIYLLDITVFQMRLQFVKGGDIITDKKEMRNNYLKSRRFKMDLLCLLPLDFLYLKLGINPLLRLPRCLKYMAFFEFNNRLEAILSKAYVYRVIRTTAYLLYSLHLNSCLYYWASAFQGIGSTHWVYDGVGNSYIRCYYWAVKTLITIGGLPDPQTLFEIVFQLLNYFTGVFAFSVMIGQMRDVVGAATAGQTYYRSCMDSTVKYMNFYKIPRSVQNRVKTWYEYTWHSQGMLDESELMVQLPDKMRLDLAIDVNYSIVSKVALFQGCDRQMIFDMLKRLRSVVYLPNDYVCKKGEIGREMYIIQAGQVQVLGGPDGKAVLVTLKAGSVFGEISLLAVGGGNRRTANVVAHGFTNLFILDKKDLNEILVHYPESQKLLRKKARRMLRNNNKPKEEKSVLILPPRAGTPKLFNAALAAAGKMGPRGAKGGKLAHLRARLKELAALEAAARQQQLLEQAKSSQEAGGEEGSGATDQPAPQEPPEPKDPPKPPGPPEPSAQSSPPPASAKPEESTGEAAGPPEPSVRIRVSPGPDPGEQTLSVEVLEEKKEGAE

Target ORF information:

RefSeq Version	XM_006531140.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclic nucleotide gated channel beta 1 (Cngb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531140.2

ORF Insert Sequence:

ATGGCTAAAC CAGGCCTGAA CATGGAGCTG AATCGCCTGG TTCAGGACCA GCCACCTGGC 
CAGGAGGGCC CCAGACCTGG CCCCCCAAAC CCAGCCGAGC ACCTCCCCAA TGTGCCCAGC 
TATCGGCCCG CAACCACCCG CATCCCTGTC CTCGTTTCCC GGAGGACGGC CTTGTCCAAC 
TCCAACTTCA CCAAGGAGAT CAGGAGCTCC ATCCGTCGCC TGGTACCAGC CACAAAGGAG 
CACCCGGAGC TCCAGGTAGA AGACACGGAT GCTGATAGTG GCCCCCTCAT CCCAGAGGAG 
ACACTCCCTC CACCTGAGAG ACCACCACCG TCTCCTGTCA AGTCGGACAC CCTCACAGTT 
CCCGGCGCCG CTGCAGCAGG CCACAGGAAG AAGCTACCTT CTCAGGATGA TGAGGCCGAA 
GAGCTCAAGG CCCTGTCACC AGCTGAGTCC CCAGTGGTTG CCTGGTCGGA CCCCACCACC 
CCACAGGAGG CTGATGGCCA GGACCGAGCA GCCTCCACCG CCAGCCAGAA CAGTGCCATC 
ATCAATGACC GGCTCCAGGA GCTGGTGAAG ATGTTCAAGG AGCGGACGGA GAAGGTGAAG 
GAGAAGCTCA TTGACCCTGA CGTCACCTCT GATGAGGAGA GCCCCAAGCC CTCCCCGGCC 
AAGAAGGCCC CAGAACCAGA CCCAGCCCAG AAGCCAGCGG AGGCAGAGGT GGCAGAGGAG 
GAACACTACT GCGACATGCT CTGCTGCAAG TTCAAGCGCA GGCCCTTGAA GATGTACCGG 
TTCCCCCAGA GTATCGACCC ACTGACCAAC CTCATGTACA TCTTGTGGCT GTTCTTTGTG 
GTGCTGGCCT GGAACTGGAA CTGCTGGCTG ATCCCTGTGC GCTGGGCCTT CCCGTACCAG 
CGGGCAGACA ACATCCACTT CTGGCTCCTC ATGGATTACT TGTGCGACTT CATCTACCTC 
CTGGACATCA CCGTGTTCCA GATGCGCCTG CAGTTTGTCA AAGGCGGGGA CATCATTACA 
GACAAGAAGG AGATGCGTAA TAACTACCTG AAGTCTCGCC GGTTTAAGAT GGACCTGCTC 
TGCCTCCTGC CCTTGGACTT TCTCTACTTG AAACTTGGCA TCAACCCTCT CCTTCGCCTG 
CCCCGCTGCC TGAAGTACAT GGCCTTCTTT GAGTTTAATA ACCGCCTGGA AGCCATCCTC 
AGCAAAGCCT ACGTTTACAG AGTCATCAGG ACCACGGCCT ACCTGCTGTA CAGCCTGCAC 
CTCAACTCCT GCCTTTACTA CTGGGCATCA GCCTTCCAGG GCATCGGCTC CACTCACTGG 
GTTTACGACG GAGTGGGGAA CAGTTATATT CGATGCTACT ACTGGGCTGT GAAAACCCTC 
ATCACCATCG GAGGACTGCC CGACCCCCAG ACGCTCTTTG AGATTGTCTT CCAGCTGCTG 
AACTATTTTA CCGGGGTCTT TGCTTTCTCT GTGATGATTG GACAGATGAG AGATGTGGTG 
GGGGCCGCCA CAGCGGGGCA GACCTACTAC CGCAGCTGCA TGGACAGCAC AGTGAAGTAC 
ATGAACTTCT ACAAGATCCC CAGGTCTGTG CAGAACCGTG TCAAGACCTG GTATGAGTAC 
ACCTGGCATT CGCAAGGCAT GCTGGATGAG TCAGAGCTGA TGGTACAACT TCCGGACAAG 
ATGCGACTGG ACCTGGCCAT TGATGTGAAC TACAGTATTG TCAGCAAAGT GGCCCTCTTT 
CAGGGCTGTG ACCGGCAGAT GATCTTCGAC ATGCTCAAGC GGCTTCGTTC TGTTGTCTAC 
CTACCCAATG ACTATGTGTG CAAGAAGGGG GAGATTGGCC GAGAGATGTA CATTATCCAG 
GCGGGGCAGG TGCAGGTGCT GGGCGGCCCA GATGGGAAGG CTGTACTGGT GACACTCAAA 
GCCGGATCGG TGTTTGGAGA AATAAGCTTG CTGGCTGTCG GGGGTGGCAA CCGGCGCACA 
GCCAACGTGG TGGCCCATGG CTTCACCAAC CTCTTCATAC TGGACAAGAA GGACTTGAAT 
GAAATTTTAG TGCATTACCC GGAATCTCAG AAGTTACTTC GGAAGAAGGC CAGGCGCATG 
CTAAGAAATA ACAACAAACC CAAGGAGGAA AAGAGTGTGC TCATCCTGCC CCCGCGTGCG 
GGCACCCCGA AGCTCTTCAA TGCTGCCCTG GCTGCTGCAG GAAAGATGGG CCCCAGGGGA 
GCCAAGGGCG GCAAGCTCGC CCACCTGAGA GCCAGGCTCA AGGAACTGGC TGCACTGGAG 
GCAGCCGCGC GACAGCAACA GCTGCTGGAA CAGGCCAAGA GCTCGCAAGA AGCTGGGGGA 
GAGGAGGGCT CTGGGGCCAC AGACCAACCT GCACCCCAGG AGCCACCAGA GCCCAAGGAC 
CCTCCGAAGC CACCAGGGCC ACCAGAGCCC TCAGCCCAGA GCTCTCCACC TCCAGCCTCA 
GCAAAGCCCG AGGAAAGCAC GGGGGAGGCA GCAGGGCCCC CGGAGCCCTC GGTGAGGATC 
CGTGTGAGTC CAGGCCCTGA TCCCGGGGAG CAGACACTGT CGGTGGAGGT GCTGGAGGAG 
AAGAAAGAGG GGGCGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cngb1 ( XM_006531140.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531203.1 Mus musculus cyclic nucleotide gated channel beta 1 (Cngb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu41571
Clone ID Related Accession (Same CDS sequence)	XM_006531140.2 , XM_006531140.3
Accession Version	XM_006531140.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cyclic nucleotide-gated cation channel beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006531140.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAAC CAGGCCTGAA CATGGAGCTG AATCGCCTGG TTCAGGACCA GCCACCTGGC 
CAGGAGGGCC CCAGACCTGG CCCCCCAAAC CCAGCCGAGC ACCTCCCCAA TGTGCCCAGC 
TATCGGCCCG CAACCACCCG CATCCCTGTC CTCGTTTCCC GGAGGACGGC CTTGTCCAAC 
TCCAACTTCA CCAAGGAGAT CAGGAGCTCC ATCCGTCGCC TGGTACCAGC CACAAAGGAG 
CACCCGGAGC TCCAGGTAGA AGACACGGAT GCTGATAGTG GCCCCCTCAT CCCAGAGGAG 
ACACTCCCTC CACCTGAGAG ACCACCACCG TCTCCTGTCA AGTCGGACAC CCTCACAGTT 
CCCGGCGCCG CTGCAGCAGG CCACAGGAAG AAGCTACCTT CTCAGGATGA TGAGGCCGAA 
GAGCTCAAGG CCCTGTCACC AGCTGAGTCC CCAGTGGTTG CCTGGTCGGA CCCCACCACC 
CCACAGGAGG CTGATGGCCA GGACCGAGCA GCCTCCACCG CCAGCCAGAA CAGTGCCATC 
ATCAATGACC GGCTCCAGGA GCTGGTGAAG ATGTTCAAGG AGCGGACGGA GAAGGTGAAG 
GAGAAGCTCA TTGACCCTGA CGTCACCTCT GATGAGGAGA GCCCCAAGCC CTCCCCGGCC 
AAGAAGGCCC CAGAACCAGA CCCAGCCCAG AAGCCAGCGG AGGCAGAGGT GGCAGAGGAG 
GAACACTACT GCGACATGCT CTGCTGCAAG TTCAAGCGCA GGCCCTTGAA GATGTACCGG 
TTCCCCCAGA GTATCGACCC ACTGACCAAC CTCATGTACA TCTTGTGGCT GTTCTTTGTG 
GTGCTGGCCT GGAACTGGAA CTGCTGGCTG ATCCCTGTGC GCTGGGCCTT CCCGTACCAG 
CGGGCAGACA ACATCCACTT CTGGCTCCTC ATGGATTACT TGTGCGACTT CATCTACCTC 
CTGGACATCA CCGTGTTCCA GATGCGCCTG CAGTTTGTCA AAGGCGGGGA CATCATTACA 
GACAAGAAGG AGATGCGTAA TAACTACCTG AAGTCTCGCC GGTTTAAGAT GGACCTGCTC 
TGCCTCCTGC CCTTGGACTT TCTCTACTTG AAACTTGGCA TCAACCCTCT CCTTCGCCTG 
CCCCGCTGCC TGAAGTACAT GGCCTTCTTT GAGTTTAATA ACCGCCTGGA AGCCATCCTC 
AGCAAAGCCT ACGTTTACAG AGTCATCAGG ACCACGGCCT ACCTGCTGTA CAGCCTGCAC 
CTCAACTCCT GCCTTTACTA CTGGGCATCA GCCTTCCAGG GCATCGGCTC CACTCACTGG 
GTTTACGACG GAGTGGGGAA CAGTTATATT CGATGCTACT ACTGGGCTGT GAAAACCCTC 
ATCACCATCG GAGGACTGCC CGACCCCCAG ACGCTCTTTG AGATTGTCTT CCAGCTGCTG 
AACTATTTTA CCGGGGTCTT TGCTTTCTCT GTGATGATTG GACAGATGAG AGATGTGGTG 
GGGGCCGCCA CAGCGGGGCA GACCTACTAC CGCAGCTGCA TGGACAGCAC AGTGAAGTAC 
ATGAACTTCT ACAAGATCCC CAGGTCTGTG CAGAACCGTG TCAAGACCTG GTATGAGTAC 
ACCTGGCATT CGCAAGGCAT GCTGGATGAG TCAGAGCTGA TGGTACAACT TCCGGACAAG 
ATGCGACTGG ACCTGGCCAT TGATGTGAAC TACAGTATTG TCAGCAAAGT GGCCCTCTTT 
CAGGGCTGTG ACCGGCAGAT GATCTTCGAC ATGCTCAAGC GGCTTCGTTC TGTTGTCTAC 
CTACCCAATG ACTATGTGTG CAAGAAGGGG GAGATTGGCC GAGAGATGTA CATTATCCAG 
GCGGGGCAGG TGCAGGTGCT GGGCGGCCCA GATGGGAAGG CTGTACTGGT GACACTCAAA 
GCCGGATCGG TGTTTGGAGA AATAAGCTTG CTGGCTGTCG GGGGTGGCAA CCGGCGCACA 
GCCAACGTGG TGGCCCATGG CTTCACCAAC CTCTTCATAC TGGACAAGAA GGACTTGAAT 
GAAATTTTAG TGCATTACCC GGAATCTCAG AAGTTACTTC GGAAGAAGGC CAGGCGCATG 
CTAAGAAATA ACAACAAACC CAAGGAGGAA AAGAGTGTGC TCATCCTGCC CCCGCGTGCG 
GGCACCCCGA AGCTCTTCAA TGCTGCCCTG GCTGCTGCAG GAAAGATGGG CCCCAGGGGA 
GCCAAGGGCG GCAAGCTCGC CCACCTGAGA GCCAGGCTCA AGGAACTGGC TGCACTGGAG 
GCAGCCGCGC GACAGCAACA GCTGCTGGAA CAGGCCAAGA GCTCGCAAGA AGCTGGGGGA 
GAGGAGGGCT CTGGGGCCAC AGACCAACCT GCACCCCAGG AGCCACCAGA GCCCAAGGAC 
CCTCCGAAGC CACCAGGGCC ACCAGAGCCC TCAGCCCAGA GCTCTCCACC TCCAGCCTCA 
GCAAAGCCCG AGGAAAGCAC GGGGGAGGCA GCAGGGCCCC CGGAGCCCTC GGTGAGGATC 
CGTGTGAGTC CAGGCCCTGA TCCCGGGGAG CAGACACTGT CGGTGGAGGT GCTGGAGGAG 
AAGAAAGAGG GGGCGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531203.1
CDS	115..2715
Translation	MAKPGLNMELNRLVQDQPPGQEGPRPGPPNPAEHLPNVPSYRPATTRIPVLVSRRTALSNSNFTKEIRSSIRRLVPATKEHPELQVEDTDADSGPLIPEETLPPPERPPPSPVKSDTLTVPGAAAAGHRKKLPSQDDEAEELKALSPAESPVVAWSDPTTPQEADGQDRAASTASQNSAIINDRLQELVKMFKERTEKVKEKLIDPDVTSDEESPKPSPAKKAPEPDPAQKPAEAEVAEEEHYCDMLCCKFKRRPLKMYRFPQSIDPLTNLMYILWLFFVVLAWNWNCWLIPVRWAFPYQRADNIHFWLLMDYLCDFIYLLDITVFQMRLQFVKGGDIITDKKEMRNNYLKSRRFKMDLLCLLPLDFLYLKLGINPLLRLPRCLKYMAFFEFNNRLEAILSKAYVYRVIRTTAYLLYSLHLNSCLYYWASAFQGIGSTHWVYDGVGNSYIRCYYWAVKTLITIGGLPDPQTLFEIVFQLLNYFTGVFAFSVMIGQMRDVVGAATAGQTYYRSCMDSTVKYMNFYKIPRSVQNRVKTWYEYTWHSQGMLDESELMVQLPDKMRLDLAIDVNYSIVSKVALFQGCDRQMIFDMLKRLRSVVYLPNDYVCKKGEIGREMYIIQAGQVQVLGGPDGKAVLVTLKAGSVFGEISLLAVGGGNRRTANVVAHGFTNLFILDKKDLNEILVHYPESQKLLRKKARRMLRNNNKPKEEKSVLILPPRAGTPKLFNAALAAAGKMGPRGAKGGKLAHLRARLKELAALEAAARQQQLLEQAKSSQEAGGEEGSGATDQPAPQEPPEPKDPPKPPGPPEPSAQSSPPPASAKPEESTGEAAGPPEPSVRIRVSPGPDPGEQTLSVEVLEEKKEGAE

Target ORF information:

RefSeq Version	XM_006531140.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclic nucleotide gated channel beta 1 (Cngb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531140.3

ORF Insert Sequence:

ATGGCTAAAC CAGGCCTGAA CATGGAGCTG AATCGCCTGG TTCAGGACCA GCCACCTGGC 
CAGGAGGGCC CCAGACCTGG CCCCCCAAAC CCAGCCGAGC ACCTCCCCAA TGTGCCCAGC 
TATCGGCCCG CAACCACCCG CATCCCTGTC CTCGTTTCCC GGAGGACGGC CTTGTCCAAC 
TCCAACTTCA CCAAGGAGAT CAGGAGCTCC ATCCGTCGCC TGGTACCAGC CACAAAGGAG 
CACCCGGAGC TCCAGGTAGA AGACACGGAT GCTGATAGTG GCCCCCTCAT CCCAGAGGAG 
ACACTCCCTC CACCTGAGAG ACCACCACCG TCTCCTGTCA AGTCGGACAC CCTCACAGTT 
CCCGGCGCCG CTGCAGCAGG CCACAGGAAG AAGCTACCTT CTCAGGATGA TGAGGCCGAA 
GAGCTCAAGG CCCTGTCACC AGCTGAGTCC CCAGTGGTTG CCTGGTCGGA CCCCACCACC 
CCACAGGAGG CTGATGGCCA GGACCGAGCA GCCTCCACCG CCAGCCAGAA CAGTGCCATC 
ATCAATGACC GGCTCCAGGA GCTGGTGAAG ATGTTCAAGG AGCGGACGGA GAAGGTGAAG 
GAGAAGCTCA TTGACCCTGA CGTCACCTCT GATGAGGAGA GCCCCAAGCC CTCCCCGGCC 
AAGAAGGCCC CAGAACCAGA CCCAGCCCAG AAGCCAGCGG AGGCAGAGGT GGCAGAGGAG 
GAACACTACT GCGACATGCT CTGCTGCAAG TTCAAGCGCA GGCCCTTGAA GATGTACCGG 
TTCCCCCAGA GTATCGACCC ACTGACCAAC CTCATGTACA TCTTGTGGCT GTTCTTTGTG 
GTGCTGGCCT GGAACTGGAA CTGCTGGCTG ATCCCTGTGC GCTGGGCCTT CCCGTACCAG 
CGGGCAGACA ACATCCACTT CTGGCTCCTC ATGGATTACT TGTGCGACTT CATCTACCTC 
CTGGACATCA CCGTGTTCCA GATGCGCCTG CAGTTTGTCA AAGGCGGGGA CATCATTACA 
GACAAGAAGG AGATGCGTAA TAACTACCTG AAGTCTCGCC GGTTTAAGAT GGACCTGCTC 
TGCCTCCTGC CCTTGGACTT TCTCTACTTG AAACTTGGCA TCAACCCTCT CCTTCGCCTG 
CCCCGCTGCC TGAAGTACAT GGCCTTCTTT GAGTTTAATA ACCGCCTGGA AGCCATCCTC 
AGCAAAGCCT ACGTTTACAG AGTCATCAGG ACCACGGCCT ACCTGCTGTA CAGCCTGCAC 
CTCAACTCCT GCCTTTACTA CTGGGCATCA GCCTTCCAGG GCATCGGCTC CACTCACTGG 
GTTTACGACG GAGTGGGGAA CAGTTATATT CGATGCTACT ACTGGGCTGT GAAAACCCTC 
ATCACCATCG GAGGACTGCC CGACCCCCAG ACGCTCTTTG AGATTGTCTT CCAGCTGCTG 
AACTATTTTA CCGGGGTCTT TGCTTTCTCT GTGATGATTG GACAGATGAG AGATGTGGTG 
GGGGCCGCCA CAGCGGGGCA GACCTACTAC CGCAGCTGCA TGGACAGCAC AGTGAAGTAC 
ATGAACTTCT ACAAGATCCC CAGGTCTGTG CAGAACCGTG TCAAGACCTG GTATGAGTAC 
ACCTGGCATT CGCAAGGCAT GCTGGATGAG TCAGAGCTGA TGGTACAACT TCCGGACAAG 
ATGCGACTGG ACCTGGCCAT TGATGTGAAC TACAGTATTG TCAGCAAAGT GGCCCTCTTT 
CAGGGCTGTG ACCGGCAGAT GATCTTCGAC ATGCTCAAGC GGCTTCGTTC TGTTGTCTAC 
CTACCCAATG ACTATGTGTG CAAGAAGGGG GAGATTGGCC GAGAGATGTA CATTATCCAG 
GCGGGGCAGG TGCAGGTGCT GGGCGGCCCA GATGGGAAGG CTGTACTGGT GACACTCAAA 
GCCGGATCGG TGTTTGGAGA AATAAGCTTG CTGGCTGTCG GGGGTGGCAA CCGGCGCACA 
GCCAACGTGG TGGCCCATGG CTTCACCAAC CTCTTCATAC TGGACAAGAA GGACTTGAAT 
GAAATTTTAG TGCATTACCC GGAATCTCAG AAGTTACTTC GGAAGAAGGC CAGGCGCATG 
CTAAGAAATA ACAACAAACC CAAGGAGGAA AAGAGTGTGC TCATCCTGCC CCCGCGTGCG 
GGCACCCCGA AGCTCTTCAA TGCTGCCCTG GCTGCTGCAG GAAAGATGGG CCCCAGGGGA 
GCCAAGGGCG GCAAGCTCGC CCACCTGAGA GCCAGGCTCA AGGAACTGGC TGCACTGGAG 
GCAGCCGCGC GACAGCAACA GCTGCTGGAA CAGGCCAAGA GCTCGCAAGA AGCTGGGGGA 
GAGGAGGGCT CTGGGGCCAC AGACCAACCT GCACCCCAGG AGCCACCAGA GCCCAAGGAC 
CCTCCGAAGC CACCAGGGCC ACCAGAGCCC TCAGCCCAGA GCTCTCCACC TCCAGCCTCA 
GCAAAGCCCG AGGAAAGCAC GGGGGAGGCA GCAGGGCCCC CGGAGCCCTC GGTGAGGATC 
CGTGTGAGTC CAGGCCCTGA TCCCGGGGAG CAGACACTGT CGGTGGAGGT GCTGGAGGAG 
AAGAAAGAGG GGGCGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cngb1 ( NM_145601.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663576.1 Mus musculus cyclic nucleotide gated channel beta 1 (Cngb1), transcript variant 2, mRNA.

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CloneID	OMu11862
Clone ID Related Accession (Same CDS sequence)	NM_145601.1
Accession Version	NM_145601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	cyclic nucleotide-gated cation channel beta-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC016201.1. On Sep 9, 2010 this sequence version replaced XM_001476198.1. Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016201.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTTGGGCT GGGTCCAAAG GGTGCTGCCT CAGCCTCCGG GGACCCCTCA GAAGACCGTG 
GAGACAGCAG GACCACAGCC AGAAACAGAG TCGAAACCAG AGGCCAACCC ACAACCGGAG 
CCAGAACCTC AGCAGGAACC AGAGCCAGAG CCAGAGCCAG AGCCAGAGCC AGAGCCAGAG 
CCAGAGCCAG AGCCAGAGCC AGAGCCAGAA CCTGAACCTG AACCAGTACC TGAAGAGGCT 
CCACCTGAGG TCCAGGCCCT GCCACCAGAG GAGCCAATGG AAGGAGAGGG AGAGGCTGAG 
GCTGGTCCCA GCCTTCAAGA GACCCAGGTA GCTGACCCTG CTCAGCCCAC CTCCCAGGCC 
CAGGTTGCTG TTGCCAAGGT GAACAGGCCC AGCTCCTGGA TGTTGAGCTG GTTCTGGAGA 
GGCATGCAGA AGGTCGTGCC ACAGCCTGTC TGCAGCAATG GGGGCCAGAA CCTGGCTGCC 
GGAGAGCGAG ACCCAGATCA GGGTGGAGCA CAGATCCCAG AACCTTGTGG CACTGGGGAC 
CCAGGATCTG CAGAAGCTTC CGGGACTCAG GACACTGAGC CCAGCCTTTG GCTGCTCAGG 
TGGCTTGAGC AGAATCTGGA AAAAGTGCTG CCCCAGCCCC CTCCGCCTTC CCTGGCCTGG 
AAAGTTGAAC CTGAGGCTGC TGTCTTGGAT CCAGATCCTC CAGGAACCCC TATGCAGATG 
GAGCCCACAG AGAGCCCCTC CCAGCCTAAT CCTGGACCCC TGGAGCCTGA GGAAGAGCCA 
GCTGCAGAGC CCCAGCCTGG CTTCCAATCC TCTTCCCTGC CACCACCCGG GGACCCCGTC 
AGGCTGATTG AGTGGCTCCT ACACAGACTG GAGATGGCCC TGCCTCAGCC CGTGCTCCAT 
GGGAAGGCTG CAGAGCAAGA GCCTGGCTGC CCTGGGATGT GTGACGTACA GACCAGGGCG 
ACAGCAGCTG GAGGCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663576.1
CDS	63..1043
Translation	MLGWVQRVLPQPPGTPQKTVETAGPQPETESKPEANPQPEPEPQQEPEPEPEPEPEPEPEPEPEPEPEPEPEPEPVPEEAPPEVQALPPEEPMEGEGEAEAGPSLQETQVADPAQPTSQAQVAVAKVNRPSSWMLSWFWRGMQKVVPQPVCSNGGQNLAAGERDPDQGGAQIPEPCGTGDPGSAEASGTQDTEPSLWLLRWLEQNLEKVLPQPPPPSLAWKVEPEAAVLDPDPPGTPMQMEPTESPSQPNPGPLEPEEEPAAEPQPGFQSSSLPPPGDPVRLIEWLLHRLEMALPQPVLHGKAAEQEPGCPGMCDVQTRATAAGGL

Target ORF information:

RefSeq Version	NM_145601.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclic nucleotide gated channel beta 1 (Cngb1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145601.1

ORF Insert Sequence:

ATGTTGGGCT GGGTCCAAAG GGTGCTGCCT CAGCCTCCGG GGACCCCTCA GAAGACCGTG 
GAGACAGCAG GACCACAGCC AGAAACAGAG TCGAAACCAG AGGCCAACCC ACAACCGGAG 
CCAGAACCTC AGCAGGAACC AGAGCCAGAG CCAGAGCCAG AGCCAGAGCC AGAGCCAGAG 
CCAGAGCCAG AGCCAGAGCC AGAGCCAGAA CCTGAACCTG AACCAGTACC TGAAGAGGCT 
CCACCTGAGG TCCAGGCCCT GCCACCAGAG GAGCCAATGG AAGGAGAGGG AGAGGCTGAG 
GCTGGTCCCA GCCTTCAAGA GACCCAGGTA GCTGACCCTG CTCAGCCCAC CTCCCAGGCC 
CAGGTTGCTG TTGCCAAGGT GAACAGGCCC AGCTCCTGGA TGTTGAGCTG GTTCTGGAGA 
GGCATGCAGA AGGTCGTGCC ACAGCCTGTC TGCAGCAATG GGGGCCAGAA CCTGGCTGCC 
GGAGAGCGAG ACCCAGATCA GGGTGGAGCA CAGATCCCAG AACCTTGTGG CACTGGGGAC 
CCAGGATCTG CAGAAGCTTC CGGGACTCAG GACACTGAGC CCAGCCTTTG GCTGCTCAGG 
TGGCTTGAGC AGAATCTGGA AAAAGTGCTG CCCCAGCCCC CTCCGCCTTC CCTGGCCTGG 
AAAGTTGAAC CTGAGGCTGC TGTCTTGGAT CCAGATCCTC CAGGAACCCC TATGCAGATG 
GAGCCCACAG AGAGCCCCTC CCAGCCTAAT CCTGGACCCC TGGAGCCTGA GGAAGAGCCA 
GCTGCAGAGC CCCAGCCTGG CTTCCAATCC TCTTCCCTGC CACCACCCGG GGACCCCGTC 
AGGCTGATTG AGTGGCTCCT ACACAGACTG GAGATGGCCC TGCCTCAGCC CGTGCTCCAT 
GGGAAGGCTG CAGAGCAAGA GCCTGGCTGC CCTGGGATGT GTGACGTACA GACCAGGGCG 
ACAGCAGCTG GAGGCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cngb1 ( NM_001195413.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001182342.1 Mus musculus cyclic nucleotide gated channel beta 1 (Cngb1), transcript variant 1, mRNA.

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CloneID	OMu17438
Clone ID Related Accession (Same CDS sequence)	NM_001195413.1
Accession Version	NM_001195413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	cyclic nucleotide-gated cation channel beta-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC016201.1, AC102518.11, BC045114.1 and BC046523.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HQ116386.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTTGGGCT GGGTCCAAAG GGTGCTGCCT CAGCCTCCGG GGACCCCTCA GAAGACCGTG 
GAGACAGCAG GACCACAGCC AGAAACAGAG TCGAAACCAG AGGCCAACCC ACAACCGGAG 
CCAGAACCTC AGCAGGAACC AGAGCCAGAG CCAGAGCCAG AGCCAGAGCC AGAGCCAGAG 
CCAGAGCCAG AGCCAGAGCC AGAGCCAGAA CCTGAACCTG AACCAGTACC TGAAGAGGCT 
CCACCTGAGG TCCAGGCCCT GCCACCAGAG GAGCCAATGG AAGGAGAGGG AGAGGCTGAG 
GCTGGTCCCA GCCTTCAAGA GACCCAGGTA GCTGACCCTG CTCAGCCCAC CTCCCAGGCC 
CAGGTTGCTG TTGCCAAGGT GAACAGGCCC AGCTCCTGGA TGTTGAGCTG GTTCTGGAGA 
GGCATGCAGA AGGTCGTGCC ACAGCCTGTC TGCAGCAATG GGGGCCAGAA CCTGGCTGCC 
GGAGAGCGAG ACCCAGATCA GGGTGGAGCA CAGATCCCAG AACCTTGTGG CACTGGGGAC 
CCAGGATCTG CAGAAGCTTC CGGGACTCAG GACACTGAGC CCAGCCTTTG GCTGCTCAGG 
TGGCTTGAGC AGAATCTGGA AAAAGTGCTG CCCCAGCCCC CTCCGCCTTC CCTGGCCTGG 
AAAGTTGAAC CTGAGGCTGC TGTCTTGGAT CCAGATCCTC CAGGAACCCC TATGCAGATG 
GAGCCCACAG AGAGCCCCTC CCAGCCTAAT CCTGGACCCC TGGAGCCTGA GGAAGAGCCA 
GCTGCAGAGC CCCAGCCTGG CTTCCAATCC TCTTCCCTGC CACCACCCGG GGACCCCGTC 
AGGCTGATTG AGTGGCTCCT ACACAGACTG GAGATGGCCC TGCCTCAGCC CGTGCTCCAT 
GGGAAGGCTG CAGAGCAAGA GCCTGGCTGC CCTGGGATGT GTGACGTACA GACCATCAGC 
ATCCTCCCCG TGGAACAGGT GGAACATGAT CTTGTCCTGG AGGAGGTGGA CTCTTGCTGG 
GAGGATGCTC AGCAGGAAGA TGGCGCCAGC CCGCAGGAGA CAGAGGTGGC TCCAGCTCAT 
GAAGAAGAGA GTGAGGCCAT AGTGGAGATA CCCAGGGAGC TGACGAAGAT TCAAGAAGAA 
AGAGAAGATG AGCAGGAAGA GGATGAGGAA GAGAAGGAGG AAGAGAAGAA GAAGGGGGAG 
GAAGAGAAGG AGAAGGAGGA GGAAGAGAAA GAGAAAGAGA AGGAGAAGGA GGAGGAGAAG 
GAAGAGGAAG AGAAGGAGGA GGAGGAGGAG GAAGAAAAGG AAGAGGAAGA GGAGGAAAAG 
GAAGAAGAGG AGAAGGAAGA GGAGGAGAAG GAAGAGGAGG AAGAAGAAGA GGAGGAGGAA 
GAAGAGCCTA TTGTCCTGCT GGATAGCTGT TTGGTGGTGC AGGCTGATGT GGACGAGTGC 
CAGCTAGAAA GGACACCGTC AGAGCTAGCA TCGATCCAGG AGCTGCCAGA AGAGAAAGAG 
GAGAAGGAAG AGGAGAAGGA GGAGGAGAAA GAGGAGGAGG AGGAAAAGAA GGAGGAAGAA 
GTGGAGAAGA AGGAGGAAGG GGAGGCCACA AACTCAACAG TACCAGCCAC AAAGGAGCAC 
CCGGAGCTCC AGGTAGAAGA CACGGATGCT GATAGTGGCC CCCTCATCCC AGAGGAGACA 
CTCCCTCCAC CTGAGAGACC ACCACCGTCT CCTGTCAAGT CGGACACCCT CACAGTTCCC 
GGCGCCGCTG CAGCAGGCCA CAGGAAGAAG CTACCTTCTC AGGATGATGA GGCCGAAGAG 
CTCAAGGCCC TGTCACCAGC TGAGTCCCCA GTGGTTGCCT GGTCGGACCC CACCACCCCA 
CAGGAGGCTG ATGGCCAGGA CCGAGCAGCC TCCACCGCCA GCCAGAACAG TGCCATCATC 
AATGACCGGC TCCAGGAGCT GGTGAAGATG TTCAAGGAGC GGACGGAGAA GGTGAAGGAG 
AAGCTCATTG ACCCTGACGT CACCTCTGAT GAGGAGAGCC CCAAGCCCTC CCCGGCCAAG 
AAGGCCCCAG AACCAGACCC AGCCCAGAAG CCAGCGGAGG CAGAGGTGGC AGAGGAGGAA 
CACTACTGCG ACATGCTCTG CTGCAAGTTC AAGCGCAGGC CCTTGAAGAT GTACCGGTTC 
CCCCAGAGTA TCGACCCACT GACCAACCTC ATGTACATCT TGTGGCTGTT CTTTGTGGTG 
CTGGCCTGGA ACTGGAACTG CTGGCTGATC CCTGTGCGCT GGGCCTTCCC GTACCAGCGG 
GCAGACAACA TCCACTTCTG GCTCCTCATG GATTACTTGT GCGACTTCAT CTACCTCCTG 
GACATCACCG TGTTCCAGAT GCGCCTGCAG TTTGTCAAAG GCGGGGACAT CATTACAGAC 
AAGAAGGAGA TGCGTAATAA CTACCTGAAG TCTCGCCGGT TTAAGATGGA CCTGCTCTGC 
CTCCTGCCCT TGGACTTTCT CTACTTGAAA CTTGGCATCA ACCCTCTCCT TCGCCTGCCC 
CGCTGCCTGA AGTACATGGC CTTCTTTGAG TTTAATAACC GCCTGGAAGC CATCCTCAGC 
AAAGCCTACG TTTACAGAGT CATCAGGACC ACGGCCTACC TGCTGTACAG CCTGCACCTC 
AACTCCTGCC TTTACTACTG GGCATCAGCC TTCCAGGGCA TCGGCTCCAC TCACTGGGTT 
TACGACGGAG TGGGGAACAG TTATATTCGA TGCTACTACT GGGCTGTGAA AACCCTCATC 
ACCATCGGAG GACTGCCCGA CCCCCAGACG CTCTTTGAGA TTGTCTTCCA GCTGCTGAAC 
TATTTTACCG GGGTCTTTGC TTTCTCTGTG ATGATTGGAC AGATGAGAGA TGTGGTGGGG 
GCCGCCACAG CGGGGCAGAC CTACTACCGC AGCTGCATGG ACAGCACAGT GAAGTACATG 
AACTTCTACA AGATCCCCAG GTCTGTGCAG AACCGTGTCA AGACCTGGTA TGAGTACACC 
TGGCATTCGC AAGGCATGCT GGATGAGTCA GAGCTGATGG TACAACTTCC GGACAAGATG 
CGACTGGACC TGGCCATTGA TGTGAACTAC AGTATTGTCA GCAAAGTGGC CCTCTTTCAG 
GGCTGTGACC GGCAGATGAT CTTCGACATG CTCAAGCGGC TTCGTTCTGT TGTCTACCTA 
CCCAATGACT ATGTGTGCAA GAAGGGGGAG ATTGGCCGAG AGATGTACAT TATCCAGGCG 
GGGCAGGTGC AGGTGCTGGG CGGCCCAGAT GGGAAGGCTG TACTGGTGAC ACTCAAAGCC 
GGATCGGTGT TTGGAGAAAT AAGCTTGCTG GCTGTCGGGG GTGGCAACCG GCGCACAGCC 
AACGTGGTGG CCCATGGCTT CACCAACCTC TTCATACTGG ACAAGAAGGA CTTGAATGAA 
ATTTTAGTGC ATTACCCGGA ATCTCAGAAG TTACTTCGGA AGAAGGCCAG GCGCATGCTA 
AGAAATAACA ACAAACCCAA GGAGGAAAAG AGTGTGCTCA TCCTGCCCCC GCGTGCGGGC 
ACCCCGAAGC TCTTCAATGC TGCCCTGGCT GCTGCAGGAA AGATGGGCCC CAGGGGAGCC 
AAGGGCGGCA AGCTCGCCCA CCTGAGAGCC AGGCTCAAGG AACTGGCTGC ACTGGAGGCA 
GCCGCGCGAC AGCAACAGCT GCTGGAACAG GCCAAGAGCT CGCAAGAAGC TGGGGGAGAG 
GAGGGCTCTG GGGCCACAGA CCAACCTGCA CCCCAGGAGC CACCAGAGCC CAAGGACCCT 
CCGAAGCCAC CAGGGCCACC AGAGCCCTCA GCCCAGAGCT CTCCACCTCC AGCCTCAGCA 
AAGCCCGAGG AAAGCACGGG GGAGGCAGCA GGGCCCCCGG AGCCCTCGGT GAGGATCCGT 
GTGAGTCCAG GCCCTGATCC CGGGGAGCAG ACACTGTCGG TGGAGGTGCT GGAGGAGAAG 
AAAGAGGGGG CGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182342.1
CDS	63..4040
Translation	MLGWVQRVLPQPPGTPQKTVETAGPQPETESKPEANPQPEPEPQQEPEPEPEPEPEPEPEPEPEPEPEPEPEPEPVPEEAPPEVQALPPEEPMEGEGEAEAGPSLQETQVADPAQPTSQAQVAVAKVNRPSSWMLSWFWRGMQKVVPQPVCSNGGQNLAAGERDPDQGGAQIPEPCGTGDPGSAEASGTQDTEPSLWLLRWLEQNLEKVLPQPPPPSLAWKVEPEAAVLDPDPPGTPMQMEPTESPSQPNPGPLEPEEEPAAEPQPGFQSSSLPPPGDPVRLIEWLLHRLEMALPQPVLHGKAAEQEPGCPGMCDVQTISILPVEQVEHDLVLEEVDSCWEDAQQEDGASPQETEVAPAHEEESEAIVEIPRELTKIQEEREDEQEEDEEEKEEEKKKGEEEKEKEEEEKEKEKEKEEEKEEEEKEEEEEEEKEEEEEEKEEEEKEEEEKEEEEEEEEEEEEPIVLLDSCLVVQADVDECQLERTPSELASIQELPEEKEEKEEEKEEEKEEEEEKKEEEVEKKEEGEATNSTVPATKEHPELQVEDTDADSGPLIPEETLPPPERPPPSPVKSDTLTVPGAAAAGHRKKLPSQDDEAEELKALSPAESPVVAWSDPTTPQEADGQDRAASTASQNSAIINDRLQELVKMFKERTEKVKEKLIDPDVTSDEESPKPSPAKKAPEPDPAQKPAEAEVAEEEHYCDMLCCKFKRRPLKMYRFPQSIDPLTNLMYILWLFFVVLAWNWNCWLIPVRWAFPYQRADNIHFWLLMDYLCDFIYLLDITVFQMRLQFVKGGDIITDKKEMRNNYLKSRRFKMDLLCLLPLDFLYLKLGINPLLRLPRCLKYMAFFEFNNRLEAILSKAYVYRVIRTTAYLLYSLHLNSCLYYWASAFQGIGSTHWVYDGVGNSYIRCYYWAVKTLITIGGLPDPQTLFEIVFQLLNYFTGVFAFSVMIGQMRDVVGAATAGQTYYRSCMDSTVKYMNFYKIPRSVQNRVKTWYEYTWHSQGMLDESELMVQLPDKMRLDLAIDVNYSIVSKVALFQGCDRQMIFDMLKRLRSVVYLPNDYVCKKGEIGREMYIIQAGQVQVLGGPDGKAVLVTLKAGSVFGEISLLAVGGGNRRTANVVAHGFTNLFILDKKDLNEILVHYPESQKLLRKKARRMLRNNNKPKEEKSVLILPPRAGTPKLFNAALAAAGKMGPRGAKGGKLAHLRARLKELAALEAAARQQQLLEQAKSSQEAGGEEGSGATDQPAPQEPPEPKDPPKPPGPPEPSAQSSPPPASAKPEESTGEAAGPPEPSVRIRVSPGPDPGEQTLSVEVLEEKKEGAE

Target ORF information:

RefSeq Version	NM_001195413.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclic nucleotide gated channel beta 1 (Cngb1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195413.1

ORF Insert Sequence:

ATGTTGGGCT GGGTCCAAAG GGTGCTGCCT CAGCCTCCGG GGACCCCTCA GAAGACCGTG 
GAGACAGCAG GACCACAGCC AGAAACAGAG TCGAAACCAG AGGCCAACCC ACAACCGGAG 
CCAGAACCTC AGCAGGAACC AGAGCCAGAG CCAGAGCCAG AGCCAGAGCC AGAGCCAGAG 
CCAGAGCCAG AGCCAGAGCC AGAGCCAGAA CCTGAACCTG AACCAGTACC TGAAGAGGCT 
CCACCTGAGG TCCAGGCCCT GCCACCAGAG GAGCCAATGG AAGGAGAGGG AGAGGCTGAG 
GCTGGTCCCA GCCTTCAAGA GACCCAGGTA GCTGACCCTG CTCAGCCCAC CTCCCAGGCC 
CAGGTTGCTG TTGCCAAGGT GAACAGGCCC AGCTCCTGGA TGTTGAGCTG GTTCTGGAGA 
GGCATGCAGA AGGTCGTGCC ACAGCCTGTC TGCAGCAATG GGGGCCAGAA CCTGGCTGCC 
GGAGAGCGAG ACCCAGATCA GGGTGGAGCA CAGATCCCAG AACCTTGTGG CACTGGGGAC 
CCAGGATCTG CAGAAGCTTC CGGGACTCAG GACACTGAGC CCAGCCTTTG GCTGCTCAGG 
TGGCTTGAGC AGAATCTGGA AAAAGTGCTG CCCCAGCCCC CTCCGCCTTC CCTGGCCTGG 
AAAGTTGAAC CTGAGGCTGC TGTCTTGGAT CCAGATCCTC CAGGAACCCC TATGCAGATG 
GAGCCCACAG AGAGCCCCTC CCAGCCTAAT CCTGGACCCC TGGAGCCTGA GGAAGAGCCA 
GCTGCAGAGC CCCAGCCTGG CTTCCAATCC TCTTCCCTGC CACCACCCGG GGACCCCGTC 
AGGCTGATTG AGTGGCTCCT ACACAGACTG GAGATGGCCC TGCCTCAGCC CGTGCTCCAT 
GGGAAGGCTG CAGAGCAAGA GCCTGGCTGC CCTGGGATGT GTGACGTACA GACCATCAGC 
ATCCTCCCCG TGGAACAGGT GGAACATGAT CTTGTCCTGG AGGAGGTGGA CTCTTGCTGG 
GAGGATGCTC AGCAGGAAGA TGGCGCCAGC CCGCAGGAGA CAGAGGTGGC TCCAGCTCAT 
GAAGAAGAGA GTGAGGCCAT AGTGGAGATA CCCAGGGAGC TGACGAAGAT TCAAGAAGAA 
AGAGAAGATG AGCAGGAAGA GGATGAGGAA GAGAAGGAGG AAGAGAAGAA GAAGGGGGAG 
GAAGAGAAGG AGAAGGAGGA GGAAGAGAAA GAGAAAGAGA AGGAGAAGGA GGAGGAGAAG 
GAAGAGGAAG AGAAGGAGGA GGAGGAGGAG GAAGAAAAGG AAGAGGAAGA GGAGGAAAAG 
GAAGAAGAGG AGAAGGAAGA GGAGGAGAAG GAAGAGGAGG AAGAAGAAGA GGAGGAGGAA 
GAAGAGCCTA TTGTCCTGCT GGATAGCTGT TTGGTGGTGC AGGCTGATGT GGACGAGTGC 
CAGCTAGAAA GGACACCGTC AGAGCTAGCA TCGATCCAGG AGCTGCCAGA AGAGAAAGAG 
GAGAAGGAAG AGGAGAAGGA GGAGGAGAAA GAGGAGGAGG AGGAAAAGAA GGAGGAAGAA 
GTGGAGAAGA AGGAGGAAGG GGAGGCCACA AACTCAACAG TACCAGCCAC AAAGGAGCAC 
CCGGAGCTCC AGGTAGAAGA CACGGATGCT GATAGTGGCC CCCTCATCCC AGAGGAGACA 
CTCCCTCCAC CTGAGAGACC ACCACCGTCT CCTGTCAAGT CGGACACCCT CACAGTTCCC 
GGCGCCGCTG CAGCAGGCCA CAGGAAGAAG CTACCTTCTC AGGATGATGA GGCCGAAGAG 
CTCAAGGCCC TGTCACCAGC TGAGTCCCCA GTGGTTGCCT GGTCGGACCC CACCACCCCA 
CAGGAGGCTG ATGGCCAGGA CCGAGCAGCC TCCACCGCCA GCCAGAACAG TGCCATCATC 
AATGACCGGC TCCAGGAGCT GGTGAAGATG TTCAAGGAGC GGACGGAGAA GGTGAAGGAG 
AAGCTCATTG ACCCTGACGT CACCTCTGAT GAGGAGAGCC CCAAGCCCTC CCCGGCCAAG 
AAGGCCCCAG AACCAGACCC AGCCCAGAAG CCAGCGGAGG CAGAGGTGGC AGAGGAGGAA 
CACTACTGCG ACATGCTCTG CTGCAAGTTC AAGCGCAGGC CCTTGAAGAT GTACCGGTTC 
CCCCAGAGTA TCGACCCACT GACCAACCTC ATGTACATCT TGTGGCTGTT CTTTGTGGTG 
CTGGCCTGGA ACTGGAACTG CTGGCTGATC CCTGTGCGCT GGGCCTTCCC GTACCAGCGG 
GCAGACAACA TCCACTTCTG GCTCCTCATG GATTACTTGT GCGACTTCAT CTACCTCCTG 
GACATCACCG TGTTCCAGAT GCGCCTGCAG TTTGTCAAAG GCGGGGACAT CATTACAGAC 
AAGAAGGAGA TGCGTAATAA CTACCTGAAG TCTCGCCGGT TTAAGATGGA CCTGCTCTGC 
CTCCTGCCCT TGGACTTTCT CTACTTGAAA CTTGGCATCA ACCCTCTCCT TCGCCTGCCC 
CGCTGCCTGA AGTACATGGC CTTCTTTGAG TTTAATAACC GCCTGGAAGC CATCCTCAGC 
AAAGCCTACG TTTACAGAGT CATCAGGACC ACGGCCTACC TGCTGTACAG CCTGCACCTC 
AACTCCTGCC TTTACTACTG GGCATCAGCC TTCCAGGGCA TCGGCTCCAC TCACTGGGTT 
TACGACGGAG TGGGGAACAG TTATATTCGA TGCTACTACT GGGCTGTGAA AACCCTCATC 
ACCATCGGAG GACTGCCCGA CCCCCAGACG CTCTTTGAGA TTGTCTTCCA GCTGCTGAAC 
TATTTTACCG GGGTCTTTGC TTTCTCTGTG ATGATTGGAC AGATGAGAGA TGTGGTGGGG 
GCCGCCACAG CGGGGCAGAC CTACTACCGC AGCTGCATGG ACAGCACAGT GAAGTACATG 
AACTTCTACA AGATCCCCAG GTCTGTGCAG AACCGTGTCA AGACCTGGTA TGAGTACACC 
TGGCATTCGC AAGGCATGCT GGATGAGTCA GAGCTGATGG TACAACTTCC GGACAAGATG 
CGACTGGACC TGGCCATTGA TGTGAACTAC AGTATTGTCA GCAAAGTGGC CCTCTTTCAG 
GGCTGTGACC GGCAGATGAT CTTCGACATG CTCAAGCGGC TTCGTTCTGT TGTCTACCTA 
CCCAATGACT ATGTGTGCAA GAAGGGGGAG ATTGGCCGAG AGATGTACAT TATCCAGGCG 
GGGCAGGTGC AGGTGCTGGG CGGCCCAGAT GGGAAGGCTG TACTGGTGAC ACTCAAAGCC 
GGATCGGTGT TTGGAGAAAT AAGCTTGCTG GCTGTCGGGG GTGGCAACCG GCGCACAGCC 
AACGTGGTGG CCCATGGCTT CACCAACCTC TTCATACTGG ACAAGAAGGA CTTGAATGAA 
ATTTTAGTGC ATTACCCGGA ATCTCAGAAG TTACTTCGGA AGAAGGCCAG GCGCATGCTA 
AGAAATAACA ACAAACCCAA GGAGGAAAAG AGTGTGCTCA TCCTGCCCCC GCGTGCGGGC 
ACCCCGAAGC TCTTCAATGC TGCCCTGGCT GCTGCAGGAA AGATGGGCCC CAGGGGAGCC 
AAGGGCGGCA AGCTCGCCCA CCTGAGAGCC AGGCTCAAGG AACTGGCTGC ACTGGAGGCA 
GCCGCGCGAC AGCAACAGCT GCTGGAACAG GCCAAGAGCT CGCAAGAAGC TGGGGGAGAG 
GAGGGCTCTG GGGCCACAGA CCAACCTGCA CCCCAGGAGC CACCAGAGCC CAAGGACCCT 
CCGAAGCCAC CAGGGCCACC AGAGCCCTCA GCCCAGAGCT CTCCACCTCC AGCCTCAGCA 
AAGCCCGAGG AAAGCACGGG GGAGGCAGCA GGGCCCCCGG AGCCCTCGGT GAGGATCCGT 
GTGAGTCCAG GCCCTGATCC CGGGGAGCAG ACACTGTCGG TGGAGGTGCT GGAGGAGAAG 
AAAGAGGGGG CGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Patients and animal models of CNG?1-deficient retinitis pigmentosa support gene augmentation approach.
The Journal of clinical investigation128(1)190-206(2018 Jan)
Petersen-Jones SM,Occelli LM,Winkler PA,Lee W,Sparrow JR,Tsukikawa M,Boye SL,Chiodo V,Capasso JE,Becirovic E,Sch?n C,Seeliger MW,Levin AV,Michalakis S,Hauswirth WW,Tsang SH

Two pathways of rod photoreceptor cell death induced by elevated cGMP.
Human molecular genetics26(12)2299-2306(2017 06)
Wang T,Tsang SH,Chen J

GARP2 accelerates retinal degeneration in rod cGMP-gated cation channel ?-subunit knockout mice.
Scientific reports742545(2017 Feb)
DeRamus ML,Stacks DA,Zhang Y,Huisingh CE,McGwin G,Pittler SJ

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Cngb1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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