Hcn4 cDNA ORF clone, Mus musculus(house mouse)

The following Hcn4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hcn4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu21744 NM_001081192.3
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Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $706.30
$1009.00
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OMu21744 NM_001081192.1 Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $706.30
$1009.00
OMu21744 NM_001081192.2 Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $706.30
$1009.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu21744
    Clone ID Related Accession (Same CDS sequence) NM_001081192.1 , NM_001081192.2 , NM_001081192.3
    Accession Version NM_001081192.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3606bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-30
    Organism Mus musculus(house mouse)
    Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC110530.10. On Feb 6, 2007 this sequence version replaced XM_287905.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC158106.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGGCTCTACA GCCTTCCGCA GCAGGTGGGG 
    GCCAAGGCGT GGATCATGGA CGAGGAAGAG GATGGTGAGG AAGAAGGGGC CGGGGGCCGC
    CAGGACCCCA GCCGAAGGAG CATCCGGCTG CGGCCGCTGC CCTCGCCCTC TCCCTCGGTG
    GCTGCGGGCT GCTCGGAGTC CCGGGGTGCG GCCCTCGGGG CGACAGAGAG CGAGGGGCCG
    GGCCGCAGCG CCGGCAAGTC CAGCACCAAC GGTGACTGCA GGCGCTTCCG CGGGAGTCTG
    GCCTCGCTGG GCAGCCGGGG CGGCGGCAGT GGTGGAGCAG GGGGCGGCAG CAGTCTCGGG
    CACCTGCATG ACTCCGCGGA GGAACGGCGG CTCATCGCCG CTGAGGGCGA TGCGTCCCCC
    GGCGAGGACA GGACGCCCCC GGGCCTGGCG ACCGAACCCG AGCGCCCGGC CACCGCGGCA
    CAACCCGCAG CCTCGCCGCC GCCCCAGCAG CCGCCGCAGC CGGCCTCTGC CTCCTGCGAG
    CAGCCCTCGG CGGACACCGC TATCAAAGTG GAGGGAGGCG CGGCCGCCAG CGACCAGATC
    CTCCCCGAGG CCGAGGTGCG CCTGGGCCAG AGCGGCTTCA TGCAGCGCCA GTTCGGTGCC
    ATGCTGCAAC CTGGGGTCAA CAAATTCTCC CTAAGGATGT TCGGCAGCCA GAAAGCGGTG
    GAGCGCGAGC AGGAGAGGGT TAAGTCAGCA GGGTTTTGGA TTATCCACCC CTACAGTGAC
    TTCAGATTTT ACTGGGACCT GACGATGCTG TTGCTGATGG TGGGGAATCT GATCATCATA
    CCCGTGGGCA TCACCTTCTT CAAGGATGAG AACACCACAC CCTGGATCGT CTTCAATGTG
    GTGTCAGACA CATTCTTCCT CATTGACTTG GTCCTCAACT TCCGCACGGG GATCGTGGTG
    GAGGACAACA CAGAAATCAT CCTTGACCCG CAGAGGATCA AGATGAAGTA CCTGAAAAGC
    TGGTTTGTGG TAGATTTCAT CTCCTCCATC CCTGTCGACT ACATCTTCCT TATAGTGGAG
    ACTCGCATTG ACTCGGAGGT CTACAAAACC GCTAGGGCTC TGCGCATTGT CCGTTTCACT
    AAGATCCTCA GCCTCCTGCG CCTCTTGAGG CTTTCCCGCC TCATTCGATA CATTCATCAG
    TGGGAAGAGA TCTTCCACAT GACCTATGAC CTGGCCAGCG CCGTGGTACG CATCGTGAAC
    CTCATTGGCA TGATGCTTCT GCTGTGTCAC TGGGATGGCT GCCTGCAGTT CCTAGTGCCC
    ATGCTGCAGG ACTTCCCCCA TGACTGCTGG GTGTCCATCA ATGGCATGGT GAATAACTCC
    TGGGGGAAGC AGTATTCCTA CGCCCTCTTC AAGGCCATGA GCCACATGCT GTGCATTGGG
    TATGGACGGC AGGCACCCGT AGGCATGTCT GACGTCTGGC TCACCATGCT CAGCATGATC
    GTGGGGGCCA CCTGCTATGC CATGTTCATC GGCCACGCCA CTGCCCTCAT CCAGTCGCTA
    GACTCCTCCC GGCGCCAGTA CCAGGAGAAG TATAAACAGG TGGAGCAGTA CATGTCCTTC
    CACAAGCTCC CGCCTGACAC CCGACAGCGC ATCCATGACT ACTATGAACA CCGCTACCAA
    GGCAAGATGT TTGATGAGGA AAGCATCCTG GGTGAGCTGA GTGAGCCACT TCGAGAGGAG
    ATCATCAACT TTAACTGCCG AAAGCTGGTG GCATCCATGC CACTGTTTGC CAACGCAGAT
    CCCAACTTTG TGACATCCAT GCTGACCAAG TTGCGTTTCG AGGTCTTCCA GCCTGGGGAT
    TACATCATCC GCGAAGGCAC CATCGGCAAG AAGATGTACT TTATCCAGCA CGGCGTGGTC
    AGCGTGCTCA CTAAGGGCAA CAAAGAGACC AAGCTGGCTG ATGGCTCCTA TTTTGGAGAG
    ATCTGCTTGC TGACCCGGGG TCGGCGCACA GCCAGCGTCA GAGCGGATAC TTATTGCCGC
    CTCTACTCAC TGAGCGTGGA CAACTTCAAT GAGGTGCTGG AGGAGTATCC CATGATGCGG
    AGGGCCTTCG AGACGGTTGC GCTGGACCGC CTGGACCGCA TAGGCAAGAA GAACTCCATC
    CTCCTCCACA AGGTGCAGCA CGACCTCAAC TCAGGCGTCT TCAACTACCA AGAGAACGAG
    ATCATCCAGC AGATCGTGCG GCATGACCGT GAGATGGCCC ACTGTGCTCA CCGCGTCCAG
    GCTGCCGCCT CAGCCACCCC AACCCCCACG CCTGTTATAT GGACCCCGCT GATCCAGGCG
    CCACTGCAGG CTGCTGCTGC TACTACTTCG GTGGCCATAG CCCTCACACA CCACCCCCGC
    CTGCCCGCCG CCATCTTCCG GCCCCCTCCC GGACCTGGGC TGGGCAACCT TGGGGCTGGA
    CAGACACCGA GGCACCCAAG GAGGCTGCAG TCCTTGATCC CTTCAGCTCT GGGCTCTGCT
    TCACCCGCCA GCAGCCCCTC ACAGGTGGAC ACACCGTCTT CATCCTCCTT CCACATCCAA
    CAGCTGGCTG GATTCTCTGC ACCTCCTGGA TTGAGCCCTC TCCTGCCCTC CTCTAGCTCT
    TCCCCACCTC CAGGAGCCTG CGGTTCCCCA CCAGCCCCCA CACCCTCCAC CTCCACTGCC
    GCCGCCGCCT CCACCACTGG GTTCGGCCAC TTTCACAAGG CGCTGGGTGG CTCCCTGTCA
    TCCTCTGACT CCCCGCTGCT CACCCCACTG CAACCAGGCG CTCGCTCTCC ACAGGCTGCC
    CAGCCACCAC CCCCACTGCC TGGGGCCCGA GGAGGTCTGG GACTCCTGGA GCACTTCTTG
    CCGCCCCCAC CCTCCTCCAG GTCACCATCA TCCAGCCCTG GGCAGCTGGG CCAGCCTCCT
    GGAGAGTTGT CCCTAGGTCT GGCAGCTGGT CCATCAAGTA CACCAGAGAC ACCCCCACGG
    CCTGAGCGAC CATCCTTCAT GGCAGGGGCC TCTGGAGGGG CTTCTCCTGT AGCCTTTACC
    CCCCGAGGAG GCCTCAGTCC TCCGGGCCAC AGCCCGGGGC CCCCAAGAAC TTTCCCGAGT
    GCCCCACCCC GGGCCTCTGG CTCCCATGGT TCCCTGCTCC TGCCACCTGC ATCCAGCCCT
    CCACCTCCCC AGGTCCCACA GCGCAGGGGC ACACCACCCC TCACCCCTGG CCGCCTCACA
    CAGGACCTGA AGCTCATCTC AGCCTCTCAG CCAGCCCTCC CCCAGGATGG GGCACAGACT
    CTCCGCAGGG CCTCGCCTCA CTCCTCAGGG GAGTCGGTGG CTGCCTTCTC ACTCTACCCC
    AGAGCTGGGG GTGGCAGTGG GAGTAGTGGG GGCCTTGGGC CTCCTGGAAG GCCATATGGT
    GCCATCCCAG GCCAACATGT CACTTTGCCT CGGAAGACAT CCTCAGGTTC TTTGCCACCC
    CCACTTTCTT TGTTTGGGGC AAGAGCCGCC TCTTCTGGAG GGCCCCCTCT GACTACTGCT
    GCACCCCAGA GGGAACCTGG CGCTAGGTCT GAGCCAGTAC GCTCCAAACT GCCGTCTAAT
    TTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001074661.1
    CDS1..3606
    Translation

    Target ORF information:

    RefSeq Version NM_001081192.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081192.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGGCTCTACA GCCTTCCGCA GCAGGTGGGG 
    GCCAAGGCGT GGATCATGGA CGAGGAAGAG GATGGTGAGG AAGAAGGGGC CGGGGGCCGC
    CAGGACCCCA GCCGAAGGAG CATCCGGCTG CGGCCGCTGC CCTCGCCCTC TCCCTCGGTG
    GCTGCGGGCT GCTCGGAGTC CCGGGGTGCG GCCCTCGGGG CGACAGAGAG CGAGGGGCCG
    GGCCGCAGCG CCGGCAAGTC CAGCACCAAC GGTGACTGCA GGCGCTTCCG CGGGAGTCTG
    GCCTCGCTGG GCAGCCGGGG CGGCGGCAGT GGTGGAGCAG GGGGCGGCAG CAGTCTCGGG
    CACCTGCATG ACTCCGCGGA GGAACGGCGG CTCATCGCCG CTGAGGGCGA TGCGTCCCCC
    GGCGAGGACA GGACGCCCCC GGGCCTGGCG ACCGAACCCG AGCGCCCGGC CACCGCGGCA
    CAACCCGCAG CCTCGCCGCC GCCCCAGCAG CCGCCGCAGC CGGCCTCTGC CTCCTGCGAG
    CAGCCCTCGG CGGACACCGC TATCAAAGTG GAGGGAGGCG CGGCCGCCAG CGACCAGATC
    CTCCCCGAGG CCGAGGTGCG CCTGGGCCAG AGCGGCTTCA TGCAGCGCCA GTTCGGTGCC
    ATGCTGCAAC CTGGGGTCAA CAAATTCTCC CTAAGGATGT TCGGCAGCCA GAAAGCGGTG
    GAGCGCGAGC AGGAGAGGGT TAAGTCAGCA GGGTTTTGGA TTATCCACCC CTACAGTGAC
    TTCAGATTTT ACTGGGACCT GACGATGCTG TTGCTGATGG TGGGGAATCT GATCATCATA
    CCCGTGGGCA TCACCTTCTT CAAGGATGAG AACACCACAC CCTGGATCGT CTTCAATGTG
    GTGTCAGACA CATTCTTCCT CATTGACTTG GTCCTCAACT TCCGCACGGG GATCGTGGTG
    GAGGACAACA CAGAAATCAT CCTTGACCCG CAGAGGATCA AGATGAAGTA CCTGAAAAGC
    TGGTTTGTGG TAGATTTCAT CTCCTCCATC CCTGTCGACT ACATCTTCCT TATAGTGGAG
    ACTCGCATTG ACTCGGAGGT CTACAAAACC GCTAGGGCTC TGCGCATTGT CCGTTTCACT
    AAGATCCTCA GCCTCCTGCG CCTCTTGAGG CTTTCCCGCC TCATTCGATA CATTCATCAG
    TGGGAAGAGA TCTTCCACAT GACCTATGAC CTGGCCAGCG CCGTGGTACG CATCGTGAAC
    CTCATTGGCA TGATGCTTCT GCTGTGTCAC TGGGATGGCT GCCTGCAGTT CCTAGTGCCC
    ATGCTGCAGG ACTTCCCCCA TGACTGCTGG GTGTCCATCA ATGGCATGGT GAATAACTCC
    TGGGGGAAGC AGTATTCCTA CGCCCTCTTC AAGGCCATGA GCCACATGCT GTGCATTGGG
    TATGGACGGC AGGCACCCGT AGGCATGTCT GACGTCTGGC TCACCATGCT CAGCATGATC
    GTGGGGGCCA CCTGCTATGC CATGTTCATC GGCCACGCCA CTGCCCTCAT CCAGTCGCTA
    GACTCCTCCC GGCGCCAGTA CCAGGAGAAG TATAAACAGG TGGAGCAGTA CATGTCCTTC
    CACAAGCTCC CGCCTGACAC CCGACAGCGC ATCCATGACT ACTATGAACA CCGCTACCAA
    GGCAAGATGT TTGATGAGGA AAGCATCCTG GGTGAGCTGA GTGAGCCACT TCGAGAGGAG
    ATCATCAACT TTAACTGCCG AAAGCTGGTG GCATCCATGC CACTGTTTGC CAACGCAGAT
    CCCAACTTTG TGACATCCAT GCTGACCAAG TTGCGTTTCG AGGTCTTCCA GCCTGGGGAT
    TACATCATCC GCGAAGGCAC CATCGGCAAG AAGATGTACT TTATCCAGCA CGGCGTGGTC
    AGCGTGCTCA CTAAGGGCAA CAAAGAGACC AAGCTGGCTG ATGGCTCCTA TTTTGGAGAG
    ATCTGCTTGC TGACCCGGGG TCGGCGCACA GCCAGCGTCA GAGCGGATAC TTATTGCCGC
    CTCTACTCAC TGAGCGTGGA CAACTTCAAT GAGGTGCTGG AGGAGTATCC CATGATGCGG
    AGGGCCTTCG AGACGGTTGC GCTGGACCGC CTGGACCGCA TAGGCAAGAA GAACTCCATC
    CTCCTCCACA AGGTGCAGCA CGACCTCAAC TCAGGCGTCT TCAACTACCA AGAGAACGAG
    ATCATCCAGC AGATCGTGCG GCATGACCGT GAGATGGCCC ACTGTGCTCA CCGCGTCCAG
    GCTGCCGCCT CAGCCACCCC AACCCCCACG CCTGTTATAT GGACCCCGCT GATCCAGGCG
    CCACTGCAGG CTGCTGCTGC TACTACTTCG GTGGCCATAG CCCTCACACA CCACCCCCGC
    CTGCCCGCCG CCATCTTCCG GCCCCCTCCC GGACCTGGGC TGGGCAACCT TGGGGCTGGA
    CAGACACCGA GGCACCCAAG GAGGCTGCAG TCCTTGATCC CTTCAGCTCT GGGCTCTGCT
    TCACCCGCCA GCAGCCCCTC ACAGGTGGAC ACACCGTCTT CATCCTCCTT CCACATCCAA
    CAGCTGGCTG GATTCTCTGC ACCTCCTGGA TTGAGCCCTC TCCTGCCCTC CTCTAGCTCT
    TCCCCACCTC CAGGAGCCTG CGGTTCCCCA CCAGCCCCCA CACCCTCCAC CTCCACTGCC
    GCCGCCGCCT CCACCACTGG GTTCGGCCAC TTTCACAAGG CGCTGGGTGG CTCCCTGTCA
    TCCTCTGACT CCCCGCTGCT CACCCCACTG CAACCAGGCG CTCGCTCTCC ACAGGCTGCC
    CAGCCACCAC CCCCACTGCC TGGGGCCCGA GGAGGTCTGG GACTCCTGGA GCACTTCTTG
    CCGCCCCCAC CCTCCTCCAG GTCACCATCA TCCAGCCCTG GGCAGCTGGG CCAGCCTCCT
    GGAGAGTTGT CCCTAGGTCT GGCAGCTGGT CCATCAAGTA CACCAGAGAC ACCCCCACGG
    CCTGAGCGAC CATCCTTCAT GGCAGGGGCC TCTGGAGGGG CTTCTCCTGT AGCCTTTACC
    CCCCGAGGAG GCCTCAGTCC TCCGGGCCAC AGCCCGGGGC CCCCAAGAAC TTTCCCGAGT
    GCCCCACCCC GGGCCTCTGG CTCCCATGGT TCCCTGCTCC TGCCACCTGC ATCCAGCCCT
    CCACCTCCCC AGGTCCCACA GCGCAGGGGC ACACCACCCC TCACCCCTGG CCGCCTCACA
    CAGGACCTGA AGCTCATCTC AGCCTCTCAG CCAGCCCTCC CCCAGGATGG GGCACAGACT
    CTCCGCAGGG CCTCGCCTCA CTCCTCAGGG GAGTCGGTGG CTGCCTTCTC ACTCTACCCC
    AGAGCTGGGG GTGGCAGTGG GAGTAGTGGG GGCCTTGGGC CTCCTGGAAG GCCATATGGT
    GCCATCCCAG GCCAACATGT CACTTTGCCT CGGAAGACAT CCTCAGGTTC TTTGCCACCC
    CCACTTTCTT TGTTTGGGGC AAGAGCCGCC TCTTCTGGAG GGCCCCCTCT GACTACTGCT
    GCACCCCAGA GGGAACCTGG CGCTAGGTCT GAGCCAGTAC GCTCCAAACT GCCGTCTAAT
    TTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21744
    Clone ID Related Accession (Same CDS sequence) NM_001081192.1 , NM_001081192.2 , NM_001081192.3
    Accession Version NM_001081192.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3606bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-27
    Organism Mus musculus(house mouse)
    Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110530.10. On Jun 21, 2018 this sequence version replaced NM_001081192.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC158106.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGGCTCTACA GCCTTCCGCA GCAGGTGGGG 
    GCCAAGGCGT GGATCATGGA CGAGGAAGAG GATGGTGAGG AAGAAGGGGC CGGGGGCCGC
    CAGGACCCCA GCCGAAGGAG CATCCGGCTG CGGCCGCTGC CCTCGCCCTC TCCCTCGGTG
    GCTGCGGGCT GCTCGGAGTC CCGGGGTGCG GCCCTCGGGG CGACAGAGAG CGAGGGGCCG
    GGCCGCAGCG CCGGCAAGTC CAGCACCAAC GGTGACTGCA GGCGCTTCCG CGGGAGTCTG
    GCCTCGCTGG GCAGCCGGGG CGGCGGCAGT GGTGGAGCAG GGGGCGGCAG CAGTCTCGGG
    CACCTGCATG ACTCCGCGGA GGAACGGCGG CTCATCGCCG CTGAGGGCGA TGCGTCCCCC
    GGCGAGGACA GGACGCCCCC GGGCCTGGCG ACCGAACCCG AGCGCCCGGC CACCGCGGCA
    CAACCCGCAG CCTCGCCGCC GCCCCAGCAG CCGCCGCAGC CGGCCTCTGC CTCCTGCGAG
    CAGCCCTCGG CGGACACCGC TATCAAAGTG GAGGGAGGCG CGGCCGCCAG CGACCAGATC
    CTCCCCGAGG CCGAGGTGCG CCTGGGCCAG AGCGGCTTCA TGCAGCGCCA GTTCGGTGCC
    ATGCTGCAAC CTGGGGTCAA CAAATTCTCC CTAAGGATGT TCGGCAGCCA GAAAGCGGTG
    GAGCGCGAGC AGGAGAGGGT TAAGTCAGCA GGGTTTTGGA TTATCCACCC CTACAGTGAC
    TTCAGATTTT ACTGGGACCT GACGATGCTG TTGCTGATGG TGGGGAATCT GATCATCATA
    CCCGTGGGCA TCACCTTCTT CAAGGATGAG AACACCACAC CCTGGATCGT CTTCAATGTG
    GTGTCAGACA CATTCTTCCT CATTGACTTG GTCCTCAACT TCCGCACGGG GATCGTGGTG
    GAGGACAACA CAGAAATCAT CCTTGACCCG CAGAGGATCA AGATGAAGTA CCTGAAAAGC
    TGGTTTGTGG TAGATTTCAT CTCCTCCATC CCTGTCGACT ACATCTTCCT TATAGTGGAG
    ACTCGCATTG ACTCGGAGGT CTACAAAACC GCTAGGGCTC TGCGCATTGT CCGTTTCACT
    AAGATCCTCA GCCTCCTGCG CCTCTTGAGG CTTTCCCGCC TCATTCGATA CATTCATCAG
    TGGGAAGAGA TCTTCCACAT GACCTATGAC CTGGCCAGCG CCGTGGTACG CATCGTGAAC
    CTCATTGGCA TGATGCTTCT GCTGTGTCAC TGGGATGGCT GCCTGCAGTT CCTAGTGCCC
    ATGCTGCAGG ACTTCCCCCA TGACTGCTGG GTGTCCATCA ATGGCATGGT GAATAACTCC
    TGGGGGAAGC AGTATTCCTA CGCCCTCTTC AAGGCCATGA GCCACATGCT GTGCATTGGG
    TATGGACGGC AGGCACCCGT AGGCATGTCT GACGTCTGGC TCACCATGCT CAGCATGATC
    GTGGGGGCCA CCTGCTATGC CATGTTCATC GGCCACGCCA CTGCCCTCAT CCAGTCGCTA
    GACTCCTCCC GGCGCCAGTA CCAGGAGAAG TATAAACAGG TGGAGCAGTA CATGTCCTTC
    CACAAGCTCC CGCCTGACAC CCGACAGCGC ATCCATGACT ACTATGAACA CCGCTACCAA
    GGCAAGATGT TTGATGAGGA AAGCATCCTG GGTGAGCTGA GTGAGCCACT TCGAGAGGAG
    ATCATCAACT TTAACTGCCG AAAGCTGGTG GCATCCATGC CACTGTTTGC CAACGCAGAT
    CCCAACTTTG TGACATCCAT GCTGACCAAG TTGCGTTTCG AGGTCTTCCA GCCTGGGGAT
    TACATCATCC GCGAAGGCAC CATCGGCAAG AAGATGTACT TTATCCAGCA CGGCGTGGTC
    AGCGTGCTCA CTAAGGGCAA CAAAGAGACC AAGCTGGCTG ATGGCTCCTA TTTTGGAGAG
    ATCTGCTTGC TGACCCGGGG TCGGCGCACA GCCAGCGTCA GAGCGGATAC TTATTGCCGC
    CTCTACTCAC TGAGCGTGGA CAACTTCAAT GAGGTGCTGG AGGAGTATCC CATGATGCGG
    AGGGCCTTCG AGACGGTTGC GCTGGACCGC CTGGACCGCA TAGGCAAGAA GAACTCCATC
    CTCCTCCACA AGGTGCAGCA CGACCTCAAC TCAGGCGTCT TCAACTACCA AGAGAACGAG
    ATCATCCAGC AGATCGTGCG GCATGACCGT GAGATGGCCC ACTGTGCTCA CCGCGTCCAG
    GCTGCCGCCT CAGCCACCCC AACCCCCACG CCTGTTATAT GGACCCCGCT GATCCAGGCG
    CCACTGCAGG CTGCTGCTGC TACTACTTCG GTGGCCATAG CCCTCACACA CCACCCCCGC
    CTGCCCGCCG CCATCTTCCG GCCCCCTCCC GGACCTGGGC TGGGCAACCT TGGGGCTGGA
    CAGACACCGA GGCACCCAAG GAGGCTGCAG TCCTTGATCC CTTCAGCTCT GGGCTCTGCT
    TCACCCGCCA GCAGCCCCTC ACAGGTGGAC ACACCGTCTT CATCCTCCTT CCACATCCAA
    CAGCTGGCTG GATTCTCTGC ACCTCCTGGA TTGAGCCCTC TCCTGCCCTC CTCTAGCTCT
    TCCCCACCTC CAGGAGCCTG CGGTTCCCCA CCAGCCCCCA CACCCTCCAC CTCCACTGCC
    GCCGCCGCCT CCACCACTGG GTTCGGCCAC TTTCACAAGG CGCTGGGTGG CTCCCTGTCA
    TCCTCTGACT CCCCGCTGCT CACCCCACTG CAACCAGGCG CTCGCTCTCC ACAGGCTGCC
    CAGCCACCAC CCCCACTGCC TGGGGCCCGA GGAGGTCTGG GACTCCTGGA GCACTTCTTG
    CCGCCCCCAC CCTCCTCCAG GTCACCATCA TCCAGCCCTG GGCAGCTGGG CCAGCCTCCT
    GGAGAGTTGT CCCTAGGTCT GGCAGCTGGT CCATCAAGTA CACCAGAGAC ACCCCCACGG
    CCTGAGCGAC CATCCTTCAT GGCAGGGGCC TCTGGAGGGG CTTCTCCTGT AGCCTTTACC
    CCCCGAGGAG GCCTCAGTCC TCCGGGCCAC AGCCCGGGGC CCCCAAGAAC TTTCCCGAGT
    GCCCCACCCC GGGCCTCTGG CTCCCATGGT TCCCTGCTCC TGCCACCTGC ATCCAGCCCT
    CCACCTCCCC AGGTCCCACA GCGCAGGGGC ACACCACCCC TCACCCCTGG CCGCCTCACA
    CAGGACCTGA AGCTCATCTC AGCCTCTCAG CCAGCCCTCC CCCAGGATGG GGCACAGACT
    CTCCGCAGGG CCTCGCCTCA CTCCTCAGGG GAGTCGGTGG CTGCCTTCTC ACTCTACCCC
    AGAGCTGGGG GTGGCAGTGG GAGTAGTGGG GGCCTTGGGC CTCCTGGAAG GCCATATGGT
    GCCATCCCAG GCCAACATGT CACTTTGCCT CGGAAGACAT CCTCAGGTTC TTTGCCACCC
    CCACTTTCTT TGTTTGGGGC AAGAGCCGCC TCTTCTGGAG GGCCCCCTCT GACTACTGCT
    GCACCCCAGA GGGAACCTGG CGCTAGGTCT GAGCCAGTAC GCTCCAAACT GCCGTCTAAT
    TTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001074661.1
    CDS658..4263
    Translation

    Target ORF information:

    RefSeq Version NM_001081192.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081192.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGGCTCTACA GCCTTCCGCA GCAGGTGGGG 
    GCCAAGGCGT GGATCATGGA CGAGGAAGAG GATGGTGAGG AAGAAGGGGC CGGGGGCCGC
    CAGGACCCCA GCCGAAGGAG CATCCGGCTG CGGCCGCTGC CCTCGCCCTC TCCCTCGGTG
    GCTGCGGGCT GCTCGGAGTC CCGGGGTGCG GCCCTCGGGG CGACAGAGAG CGAGGGGCCG
    GGCCGCAGCG CCGGCAAGTC CAGCACCAAC GGTGACTGCA GGCGCTTCCG CGGGAGTCTG
    GCCTCGCTGG GCAGCCGGGG CGGCGGCAGT GGTGGAGCAG GGGGCGGCAG CAGTCTCGGG
    CACCTGCATG ACTCCGCGGA GGAACGGCGG CTCATCGCCG CTGAGGGCGA TGCGTCCCCC
    GGCGAGGACA GGACGCCCCC GGGCCTGGCG ACCGAACCCG AGCGCCCGGC CACCGCGGCA
    CAACCCGCAG CCTCGCCGCC GCCCCAGCAG CCGCCGCAGC CGGCCTCTGC CTCCTGCGAG
    CAGCCCTCGG CGGACACCGC TATCAAAGTG GAGGGAGGCG CGGCCGCCAG CGACCAGATC
    CTCCCCGAGG CCGAGGTGCG CCTGGGCCAG AGCGGCTTCA TGCAGCGCCA GTTCGGTGCC
    ATGCTGCAAC CTGGGGTCAA CAAATTCTCC CTAAGGATGT TCGGCAGCCA GAAAGCGGTG
    GAGCGCGAGC AGGAGAGGGT TAAGTCAGCA GGGTTTTGGA TTATCCACCC CTACAGTGAC
    TTCAGATTTT ACTGGGACCT GACGATGCTG TTGCTGATGG TGGGGAATCT GATCATCATA
    CCCGTGGGCA TCACCTTCTT CAAGGATGAG AACACCACAC CCTGGATCGT CTTCAATGTG
    GTGTCAGACA CATTCTTCCT CATTGACTTG GTCCTCAACT TCCGCACGGG GATCGTGGTG
    GAGGACAACA CAGAAATCAT CCTTGACCCG CAGAGGATCA AGATGAAGTA CCTGAAAAGC
    TGGTTTGTGG TAGATTTCAT CTCCTCCATC CCTGTCGACT ACATCTTCCT TATAGTGGAG
    ACTCGCATTG ACTCGGAGGT CTACAAAACC GCTAGGGCTC TGCGCATTGT CCGTTTCACT
    AAGATCCTCA GCCTCCTGCG CCTCTTGAGG CTTTCCCGCC TCATTCGATA CATTCATCAG
    TGGGAAGAGA TCTTCCACAT GACCTATGAC CTGGCCAGCG CCGTGGTACG CATCGTGAAC
    CTCATTGGCA TGATGCTTCT GCTGTGTCAC TGGGATGGCT GCCTGCAGTT CCTAGTGCCC
    ATGCTGCAGG ACTTCCCCCA TGACTGCTGG GTGTCCATCA ATGGCATGGT GAATAACTCC
    TGGGGGAAGC AGTATTCCTA CGCCCTCTTC AAGGCCATGA GCCACATGCT GTGCATTGGG
    TATGGACGGC AGGCACCCGT AGGCATGTCT GACGTCTGGC TCACCATGCT CAGCATGATC
    GTGGGGGCCA CCTGCTATGC CATGTTCATC GGCCACGCCA CTGCCCTCAT CCAGTCGCTA
    GACTCCTCCC GGCGCCAGTA CCAGGAGAAG TATAAACAGG TGGAGCAGTA CATGTCCTTC
    CACAAGCTCC CGCCTGACAC CCGACAGCGC ATCCATGACT ACTATGAACA CCGCTACCAA
    GGCAAGATGT TTGATGAGGA AAGCATCCTG GGTGAGCTGA GTGAGCCACT TCGAGAGGAG
    ATCATCAACT TTAACTGCCG AAAGCTGGTG GCATCCATGC CACTGTTTGC CAACGCAGAT
    CCCAACTTTG TGACATCCAT GCTGACCAAG TTGCGTTTCG AGGTCTTCCA GCCTGGGGAT
    TACATCATCC GCGAAGGCAC CATCGGCAAG AAGATGTACT TTATCCAGCA CGGCGTGGTC
    AGCGTGCTCA CTAAGGGCAA CAAAGAGACC AAGCTGGCTG ATGGCTCCTA TTTTGGAGAG
    ATCTGCTTGC TGACCCGGGG TCGGCGCACA GCCAGCGTCA GAGCGGATAC TTATTGCCGC
    CTCTACTCAC TGAGCGTGGA CAACTTCAAT GAGGTGCTGG AGGAGTATCC CATGATGCGG
    AGGGCCTTCG AGACGGTTGC GCTGGACCGC CTGGACCGCA TAGGCAAGAA GAACTCCATC
    CTCCTCCACA AGGTGCAGCA CGACCTCAAC TCAGGCGTCT TCAACTACCA AGAGAACGAG
    ATCATCCAGC AGATCGTGCG GCATGACCGT GAGATGGCCC ACTGTGCTCA CCGCGTCCAG
    GCTGCCGCCT CAGCCACCCC AACCCCCACG CCTGTTATAT GGACCCCGCT GATCCAGGCG
    CCACTGCAGG CTGCTGCTGC TACTACTTCG GTGGCCATAG CCCTCACACA CCACCCCCGC
    CTGCCCGCCG CCATCTTCCG GCCCCCTCCC GGACCTGGGC TGGGCAACCT TGGGGCTGGA
    CAGACACCGA GGCACCCAAG GAGGCTGCAG TCCTTGATCC CTTCAGCTCT GGGCTCTGCT
    TCACCCGCCA GCAGCCCCTC ACAGGTGGAC ACACCGTCTT CATCCTCCTT CCACATCCAA
    CAGCTGGCTG GATTCTCTGC ACCTCCTGGA TTGAGCCCTC TCCTGCCCTC CTCTAGCTCT
    TCCCCACCTC CAGGAGCCTG CGGTTCCCCA CCAGCCCCCA CACCCTCCAC CTCCACTGCC
    GCCGCCGCCT CCACCACTGG GTTCGGCCAC TTTCACAAGG CGCTGGGTGG CTCCCTGTCA
    TCCTCTGACT CCCCGCTGCT CACCCCACTG CAACCAGGCG CTCGCTCTCC ACAGGCTGCC
    CAGCCACCAC CCCCACTGCC TGGGGCCCGA GGAGGTCTGG GACTCCTGGA GCACTTCTTG
    CCGCCCCCAC CCTCCTCCAG GTCACCATCA TCCAGCCCTG GGCAGCTGGG CCAGCCTCCT
    GGAGAGTTGT CCCTAGGTCT GGCAGCTGGT CCATCAAGTA CACCAGAGAC ACCCCCACGG
    CCTGAGCGAC CATCCTTCAT GGCAGGGGCC TCTGGAGGGG CTTCTCCTGT AGCCTTTACC
    CCCCGAGGAG GCCTCAGTCC TCCGGGCCAC AGCCCGGGGC CCCCAAGAAC TTTCCCGAGT
    GCCCCACCCC GGGCCTCTGG CTCCCATGGT TCCCTGCTCC TGCCACCTGC ATCCAGCCCT
    CCACCTCCCC AGGTCCCACA GCGCAGGGGC ACACCACCCC TCACCCCTGG CCGCCTCACA
    CAGGACCTGA AGCTCATCTC AGCCTCTCAG CCAGCCCTCC CCCAGGATGG GGCACAGACT
    CTCCGCAGGG CCTCGCCTCA CTCCTCAGGG GAGTCGGTGG CTGCCTTCTC ACTCTACCCC
    AGAGCTGGGG GTGGCAGTGG GAGTAGTGGG GGCCTTGGGC CTCCTGGAAG GCCATATGGT
    GCCATCCCAG GCCAACATGT CACTTTGCCT CGGAAGACAT CCTCAGGTTC TTTGCCACCC
    CCACTTTCTT TGTTTGGGGC AAGAGCCGCC TCTTCTGGAG GGCCCCCTCT GACTACTGCT
    GCACCCCAGA GGGAACCTGG CGCTAGGTCT GAGCCAGTAC GCTCCAAACT GCCGTCTAAT
    TTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21744
    Clone ID Related Accession (Same CDS sequence) NM_001081192.1 , NM_001081192.2 , NM_001081192.3
    Accession Version NM_001081192.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3606bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Mus musculus(house mouse)
    Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110530.10. On Jun 18, 2019 this sequence version replaced NM_001081192.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC158106.1, SRR7345562.1266554.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGGCTCTACA GCCTTCCGCA GCAGGTGGGG 
    GCCAAGGCGT GGATCATGGA CGAGGAAGAG GATGGTGAGG AAGAAGGGGC CGGGGGCCGC
    CAGGACCCCA GCCGAAGGAG CATCCGGCTG CGGCCGCTGC CCTCGCCCTC TCCCTCGGTG
    GCTGCGGGCT GCTCGGAGTC CCGGGGTGCG GCCCTCGGGG CGACAGAGAG CGAGGGGCCG
    GGCCGCAGCG CCGGCAAGTC CAGCACCAAC GGTGACTGCA GGCGCTTCCG CGGGAGTCTG
    GCCTCGCTGG GCAGCCGGGG CGGCGGCAGT GGTGGAGCAG GGGGCGGCAG CAGTCTCGGG
    CACCTGCATG ACTCCGCGGA GGAACGGCGG CTCATCGCCG CTGAGGGCGA TGCGTCCCCC
    GGCGAGGACA GGACGCCCCC GGGCCTGGCG ACCGAACCCG AGCGCCCGGC CACCGCGGCA
    CAACCCGCAG CCTCGCCGCC GCCCCAGCAG CCGCCGCAGC CGGCCTCTGC CTCCTGCGAG
    CAGCCCTCGG CGGACACCGC TATCAAAGTG GAGGGAGGCG CGGCCGCCAG CGACCAGATC
    CTCCCCGAGG CCGAGGTGCG CCTGGGCCAG AGCGGCTTCA TGCAGCGCCA GTTCGGTGCC
    ATGCTGCAAC CTGGGGTCAA CAAATTCTCC CTAAGGATGT TCGGCAGCCA GAAAGCGGTG
    GAGCGCGAGC AGGAGAGGGT TAAGTCAGCA GGGTTTTGGA TTATCCACCC CTACAGTGAC
    TTCAGATTTT ACTGGGACCT GACGATGCTG TTGCTGATGG TGGGGAATCT GATCATCATA
    CCCGTGGGCA TCACCTTCTT CAAGGATGAG AACACCACAC CCTGGATCGT CTTCAATGTG
    GTGTCAGACA CATTCTTCCT CATTGACTTG GTCCTCAACT TCCGCACGGG GATCGTGGTG
    GAGGACAACA CAGAAATCAT CCTTGACCCG CAGAGGATCA AGATGAAGTA CCTGAAAAGC
    TGGTTTGTGG TAGATTTCAT CTCCTCCATC CCTGTCGACT ACATCTTCCT TATAGTGGAG
    ACTCGCATTG ACTCGGAGGT CTACAAAACC GCTAGGGCTC TGCGCATTGT CCGTTTCACT
    AAGATCCTCA GCCTCCTGCG CCTCTTGAGG CTTTCCCGCC TCATTCGATA CATTCATCAG
    TGGGAAGAGA TCTTCCACAT GACCTATGAC CTGGCCAGCG CCGTGGTACG CATCGTGAAC
    CTCATTGGCA TGATGCTTCT GCTGTGTCAC TGGGATGGCT GCCTGCAGTT CCTAGTGCCC
    ATGCTGCAGG ACTTCCCCCA TGACTGCTGG GTGTCCATCA ATGGCATGGT GAATAACTCC
    TGGGGGAAGC AGTATTCCTA CGCCCTCTTC AAGGCCATGA GCCACATGCT GTGCATTGGG
    TATGGACGGC AGGCACCCGT AGGCATGTCT GACGTCTGGC TCACCATGCT CAGCATGATC
    GTGGGGGCCA CCTGCTATGC CATGTTCATC GGCCACGCCA CTGCCCTCAT CCAGTCGCTA
    GACTCCTCCC GGCGCCAGTA CCAGGAGAAG TATAAACAGG TGGAGCAGTA CATGTCCTTC
    CACAAGCTCC CGCCTGACAC CCGACAGCGC ATCCATGACT ACTATGAACA CCGCTACCAA
    GGCAAGATGT TTGATGAGGA AAGCATCCTG GGTGAGCTGA GTGAGCCACT TCGAGAGGAG
    ATCATCAACT TTAACTGCCG AAAGCTGGTG GCATCCATGC CACTGTTTGC CAACGCAGAT
    CCCAACTTTG TGACATCCAT GCTGACCAAG TTGCGTTTCG AGGTCTTCCA GCCTGGGGAT
    TACATCATCC GCGAAGGCAC CATCGGCAAG AAGATGTACT TTATCCAGCA CGGCGTGGTC
    AGCGTGCTCA CTAAGGGCAA CAAAGAGACC AAGCTGGCTG ATGGCTCCTA TTTTGGAGAG
    ATCTGCTTGC TGACCCGGGG TCGGCGCACA GCCAGCGTCA GAGCGGATAC TTATTGCCGC
    CTCTACTCAC TGAGCGTGGA CAACTTCAAT GAGGTGCTGG AGGAGTATCC CATGATGCGG
    AGGGCCTTCG AGACGGTTGC GCTGGACCGC CTGGACCGCA TAGGCAAGAA GAACTCCATC
    CTCCTCCACA AGGTGCAGCA CGACCTCAAC TCAGGCGTCT TCAACTACCA AGAGAACGAG
    ATCATCCAGC AGATCGTGCG GCATGACCGT GAGATGGCCC ACTGTGCTCA CCGCGTCCAG
    GCTGCCGCCT CAGCCACCCC AACCCCCACG CCTGTTATAT GGACCCCGCT GATCCAGGCG
    CCACTGCAGG CTGCTGCTGC TACTACTTCG GTGGCCATAG CCCTCACACA CCACCCCCGC
    CTGCCCGCCG CCATCTTCCG GCCCCCTCCC GGACCTGGGC TGGGCAACCT TGGGGCTGGA
    CAGACACCGA GGCACCCAAG GAGGCTGCAG TCCTTGATCC CTTCAGCTCT GGGCTCTGCT
    TCACCCGCCA GCAGCCCCTC ACAGGTGGAC ACACCGTCTT CATCCTCCTT CCACATCCAA
    CAGCTGGCTG GATTCTCTGC ACCTCCTGGA TTGAGCCCTC TCCTGCCCTC CTCTAGCTCT
    TCCCCACCTC CAGGAGCCTG CGGTTCCCCA CCAGCCCCCA CACCCTCCAC CTCCACTGCC
    GCCGCCGCCT CCACCACTGG GTTCGGCCAC TTTCACAAGG CGCTGGGTGG CTCCCTGTCA
    TCCTCTGACT CCCCGCTGCT CACCCCACTG CAACCAGGCG CTCGCTCTCC ACAGGCTGCC
    CAGCCACCAC CCCCACTGCC TGGGGCCCGA GGAGGTCTGG GACTCCTGGA GCACTTCTTG
    CCGCCCCCAC CCTCCTCCAG GTCACCATCA TCCAGCCCTG GGCAGCTGGG CCAGCCTCCT
    GGAGAGTTGT CCCTAGGTCT GGCAGCTGGT CCATCAAGTA CACCAGAGAC ACCCCCACGG
    CCTGAGCGAC CATCCTTCAT GGCAGGGGCC TCTGGAGGGG CTTCTCCTGT AGCCTTTACC
    CCCCGAGGAG GCCTCAGTCC TCCGGGCCAC AGCCCGGGGC CCCCAAGAAC TTTCCCGAGT
    GCCCCACCCC GGGCCTCTGG CTCCCATGGT TCCCTGCTCC TGCCACCTGC ATCCAGCCCT
    CCACCTCCCC AGGTCCCACA GCGCAGGGGC ACACCACCCC TCACCCCTGG CCGCCTCACA
    CAGGACCTGA AGCTCATCTC AGCCTCTCAG CCAGCCCTCC CCCAGGATGG GGCACAGACT
    CTCCGCAGGG CCTCGCCTCA CTCCTCAGGG GAGTCGGTGG CTGCCTTCTC ACTCTACCCC
    AGAGCTGGGG GTGGCAGTGG GAGTAGTGGG GGCCTTGGGC CTCCTGGAAG GCCATATGGT
    GCCATCCCAG GCCAACATGT CACTTTGCCT CGGAAGACAT CCTCAGGTTC TTTGCCACCC
    CCACTTTCTT TGTTTGGGGC AAGAGCCGCC TCTTCTGGAG GGCCCCCTCT GACTACTGCT
    GCACCCCAGA GGGAACCTGG CGCTAGGTCT GAGCCAGTAC GCTCCAAACT GCCGTCTAAT
    TTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001074661.1
    CDS564..4169
    Translation

    Target ORF information:

    RefSeq Version NM_001081192.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081192.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGGCTCTACA GCCTTCCGCA GCAGGTGGGG 
    GCCAAGGCGT GGATCATGGA CGAGGAAGAG GATGGTGAGG AAGAAGGGGC CGGGGGCCGC
    CAGGACCCCA GCCGAAGGAG CATCCGGCTG CGGCCGCTGC CCTCGCCCTC TCCCTCGGTG
    GCTGCGGGCT GCTCGGAGTC CCGGGGTGCG GCCCTCGGGG CGACAGAGAG CGAGGGGCCG
    GGCCGCAGCG CCGGCAAGTC CAGCACCAAC GGTGACTGCA GGCGCTTCCG CGGGAGTCTG
    GCCTCGCTGG GCAGCCGGGG CGGCGGCAGT GGTGGAGCAG GGGGCGGCAG CAGTCTCGGG
    CACCTGCATG ACTCCGCGGA GGAACGGCGG CTCATCGCCG CTGAGGGCGA TGCGTCCCCC
    GGCGAGGACA GGACGCCCCC GGGCCTGGCG ACCGAACCCG AGCGCCCGGC CACCGCGGCA
    CAACCCGCAG CCTCGCCGCC GCCCCAGCAG CCGCCGCAGC CGGCCTCTGC CTCCTGCGAG
    CAGCCCTCGG CGGACACCGC TATCAAAGTG GAGGGAGGCG CGGCCGCCAG CGACCAGATC
    CTCCCCGAGG CCGAGGTGCG CCTGGGCCAG AGCGGCTTCA TGCAGCGCCA GTTCGGTGCC
    ATGCTGCAAC CTGGGGTCAA CAAATTCTCC CTAAGGATGT TCGGCAGCCA GAAAGCGGTG
    GAGCGCGAGC AGGAGAGGGT TAAGTCAGCA GGGTTTTGGA TTATCCACCC CTACAGTGAC
    TTCAGATTTT ACTGGGACCT GACGATGCTG TTGCTGATGG TGGGGAATCT GATCATCATA
    CCCGTGGGCA TCACCTTCTT CAAGGATGAG AACACCACAC CCTGGATCGT CTTCAATGTG
    GTGTCAGACA CATTCTTCCT CATTGACTTG GTCCTCAACT TCCGCACGGG GATCGTGGTG
    GAGGACAACA CAGAAATCAT CCTTGACCCG CAGAGGATCA AGATGAAGTA CCTGAAAAGC
    TGGTTTGTGG TAGATTTCAT CTCCTCCATC CCTGTCGACT ACATCTTCCT TATAGTGGAG
    ACTCGCATTG ACTCGGAGGT CTACAAAACC GCTAGGGCTC TGCGCATTGT CCGTTTCACT
    AAGATCCTCA GCCTCCTGCG CCTCTTGAGG CTTTCCCGCC TCATTCGATA CATTCATCAG
    TGGGAAGAGA TCTTCCACAT GACCTATGAC CTGGCCAGCG CCGTGGTACG CATCGTGAAC
    CTCATTGGCA TGATGCTTCT GCTGTGTCAC TGGGATGGCT GCCTGCAGTT CCTAGTGCCC
    ATGCTGCAGG ACTTCCCCCA TGACTGCTGG GTGTCCATCA ATGGCATGGT GAATAACTCC
    TGGGGGAAGC AGTATTCCTA CGCCCTCTTC AAGGCCATGA GCCACATGCT GTGCATTGGG
    TATGGACGGC AGGCACCCGT AGGCATGTCT GACGTCTGGC TCACCATGCT CAGCATGATC
    GTGGGGGCCA CCTGCTATGC CATGTTCATC GGCCACGCCA CTGCCCTCAT CCAGTCGCTA
    GACTCCTCCC GGCGCCAGTA CCAGGAGAAG TATAAACAGG TGGAGCAGTA CATGTCCTTC
    CACAAGCTCC CGCCTGACAC CCGACAGCGC ATCCATGACT ACTATGAACA CCGCTACCAA
    GGCAAGATGT TTGATGAGGA AAGCATCCTG GGTGAGCTGA GTGAGCCACT TCGAGAGGAG
    ATCATCAACT TTAACTGCCG AAAGCTGGTG GCATCCATGC CACTGTTTGC CAACGCAGAT
    CCCAACTTTG TGACATCCAT GCTGACCAAG TTGCGTTTCG AGGTCTTCCA GCCTGGGGAT
    TACATCATCC GCGAAGGCAC CATCGGCAAG AAGATGTACT TTATCCAGCA CGGCGTGGTC
    AGCGTGCTCA CTAAGGGCAA CAAAGAGACC AAGCTGGCTG ATGGCTCCTA TTTTGGAGAG
    ATCTGCTTGC TGACCCGGGG TCGGCGCACA GCCAGCGTCA GAGCGGATAC TTATTGCCGC
    CTCTACTCAC TGAGCGTGGA CAACTTCAAT GAGGTGCTGG AGGAGTATCC CATGATGCGG
    AGGGCCTTCG AGACGGTTGC GCTGGACCGC CTGGACCGCA TAGGCAAGAA GAACTCCATC
    CTCCTCCACA AGGTGCAGCA CGACCTCAAC TCAGGCGTCT TCAACTACCA AGAGAACGAG
    ATCATCCAGC AGATCGTGCG GCATGACCGT GAGATGGCCC ACTGTGCTCA CCGCGTCCAG
    GCTGCCGCCT CAGCCACCCC AACCCCCACG CCTGTTATAT GGACCCCGCT GATCCAGGCG
    CCACTGCAGG CTGCTGCTGC TACTACTTCG GTGGCCATAG CCCTCACACA CCACCCCCGC
    CTGCCCGCCG CCATCTTCCG GCCCCCTCCC GGACCTGGGC TGGGCAACCT TGGGGCTGGA
    CAGACACCGA GGCACCCAAG GAGGCTGCAG TCCTTGATCC CTTCAGCTCT GGGCTCTGCT
    TCACCCGCCA GCAGCCCCTC ACAGGTGGAC ACACCGTCTT CATCCTCCTT CCACATCCAA
    CAGCTGGCTG GATTCTCTGC ACCTCCTGGA TTGAGCCCTC TCCTGCCCTC CTCTAGCTCT
    TCCCCACCTC CAGGAGCCTG CGGTTCCCCA CCAGCCCCCA CACCCTCCAC CTCCACTGCC
    GCCGCCGCCT CCACCACTGG GTTCGGCCAC TTTCACAAGG CGCTGGGTGG CTCCCTGTCA
    TCCTCTGACT CCCCGCTGCT CACCCCACTG CAACCAGGCG CTCGCTCTCC ACAGGCTGCC
    CAGCCACCAC CCCCACTGCC TGGGGCCCGA GGAGGTCTGG GACTCCTGGA GCACTTCTTG
    CCGCCCCCAC CCTCCTCCAG GTCACCATCA TCCAGCCCTG GGCAGCTGGG CCAGCCTCCT
    GGAGAGTTGT CCCTAGGTCT GGCAGCTGGT CCATCAAGTA CACCAGAGAC ACCCCCACGG
    CCTGAGCGAC CATCCTTCAT GGCAGGGGCC TCTGGAGGGG CTTCTCCTGT AGCCTTTACC
    CCCCGAGGAG GCCTCAGTCC TCCGGGCCAC AGCCCGGGGC CCCCAAGAAC TTTCCCGAGT
    GCCCCACCCC GGGCCTCTGG CTCCCATGGT TCCCTGCTCC TGCCACCTGC ATCCAGCCCT
    CCACCTCCCC AGGTCCCACA GCGCAGGGGC ACACCACCCC TCACCCCTGG CCGCCTCACA
    CAGGACCTGA AGCTCATCTC AGCCTCTCAG CCAGCCCTCC CCCAGGATGG GGCACAGACT
    CTCCGCAGGG CCTCGCCTCA CTCCTCAGGG GAGTCGGTGG CTGCCTTCTC ACTCTACCCC
    AGAGCTGGGG GTGGCAGTGG GAGTAGTGGG GGCCTTGGGC CTCCTGGAAG GCCATATGGT
    GCCATCCCAG GCCAACATGT CACTTTGCCT CGGAAGACAT CCTCAGGTTC TTTGCCACCC
    CCACTTTCTT TGTTTGGGGC AAGAGCCGCC TCTTCTGGAG GGCCCCCTCT GACTACTGCT
    GCACCCCAGA GGGAACCTGG CGCTAGGTCT GAGCCAGTAC GCTCCAAACT GCCGTCTAAT
    TTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.