Frem1 cDNA ORF clone, Mus musculus(house mouse)

The following Frem1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Frem1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu32989 XM_030253616.1
Latest version!
Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu32989 XM_006538017.3
Latest version!
Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu32990 XM_006538018.2
Latest version!
Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu13359 XM_006538013.4
Latest version!
Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu13359 XM_006538014.3
Latest version!
Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu13359 XM_006538015.2
Latest version!
Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu13359 XM_011250055.3
Latest version!
Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu13359 NM_001198811.2
Latest version!
Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu13403 NM_177863.5
Latest version!
Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OMu13359 XM_006538013.3 Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu13359 XM_006538014.2 Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu13359 XM_006538015.1 Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu13403 NM_177863.4 Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu13359 NM_001198811.1 Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu13359 XM_011250055.2 Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu32989
    Clone ID Related Accession (Same CDS sequence) XM_030253616.1 , XM_006538017.3
    Accession Version XM_030253616.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCCATGT TTGTTCTGGA GGTGGACCAG TTCATACTGA CCCCTCTGAC AACCTCCGTT 
    CTGGACTGTG AGGAGGATGA GACTCCGAAA CCCTTGTTGG TGTTCAACAT CACTAAAGCC
    CCACTCCAGG GCTATGTGAC CCACCTGCTG GATCATACCA GACCCATCTC TTCATTCACC
    TGGAAGGATC TCAGTGACAT GCAAGTTGCC TACCAGCCAC CAAACAGCAG CCACCCTGAG
    AGGAGACATT ACACGATGGA GCTGGAGGTG TACGACTTCT TCTTTGAGAG GAGTGCCCCC
    ATCACTGTCC ACATCTCCAT CAGAACAGCA GACACGAATG CACCACGGGT GTCTTGGAAT
    ACAGGTTTGA ATCTCCTAGA GGGGCAGTCT CGGGCCATCA CTTGGGAACA ATTTCAGATC
    GTTGACAATG ATGACATTGG TGCTGTGCAG TTGGTCACCA TTGGTGGCCT GCAGCATGGA
    CGGCTTACAG TACGAGAGGG GAAAGGATTT CTCTTCACCG TGACTGACCT TCAAGCTGGA
    GTTGTCCGCT ATCATCACGA TGACAGTGAT ACCACCAAAG ACTTTGTGGC TTTCAGGATA
    TTCGATGGCC ACCACAGCAG CCACCACAAG TTTCCTATCA ACATCTTGCC TAAAGATGAC
    AGCCCCCCAT TCCTCATCAC CAATGTCGTG ATTGAACTAG AAGAGGGGAA GACCATCTTG
    ATCCAAGGGT CTATGTTGAG AGCTTCAGAT ATGGACTCCA GTGATGACTA CATCTTCTTT
    AATATCACAA AGTTTCCACA GGCTGGGGAG ATCATGAAGA AGCCAGGGCC AAGACTGATA
    GGGTATCCTG TCCCTGGCTT CCTGCAGAGA GACCTGTTCA GTGGAATCAT TTACTATCGT
    CACTTTGGTG GAGAAATCTT TGAAGATTCC TTTGAATTCG TACTGTGGGA CAGCCATGAG
    CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATTCATA TCACTCCAGT AGATGACCAG
    CTTCCTAAAG AAGCTCCTGG CATTTCCCGG CATTTGGTTG TCAAGGAAAC AGAGGTGGCC
    TACATCACTA AAAAGCACCT ACATTTCCTA GACATGGAAT CCCGTGATGG GGAACTCATC
    TACACAGTAA CACGCCCTCC ATGTTTCTCC TTCAGCCACA GACACTTGGA TGCTGGGAAG
    CTATTCATGG TGGACAGCAT ACCAAAGCTA ACCAAAAATC CGACAGCCCC AGGGCTAAGT
    TCATTTACTC AGCATGCCGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT
    ATAGGCCCCT CCCCTCGACA TGTGCAGTTC ACAGTGTCTG TCAGTAACCA GCATGGAGGT
    GCTTTGCATG GAATCTGCTT CAACATCACA GTTCTCCCAG TGGACAACCA GGTTCCCGAG
    GTATTCACCA ACACCCTGAG AGTGGTCGAG GGCGGTCAGT GCACCATCAG CACAGAGCAC
    ATTTTGGTTT CTGATGTGGA CACCCCGCTA GACAGCATCA GCCTCTCTCT TAAGGAGAGG
    CCTCTGCATG GCGGGGTAGA GCTGGACGGA TTTCCTCTAA ACCCAAGAGG CACATTTTCT
    TGGCGAGACC TCAATACCTT AAAAGTTTGG TATCAACATG ATGGATCTGA GGTTCTTCAG
    GATGAGATAT TTTTGGAGGT CACAGATGGC ACAAATTCAG CAGCATTTGT TCTCCACATT
    GAGGTGTTCC CTGTGAATGA TGAGCCACCC ATTCTGAAGG CTGACCTCAT CCCCATGATG
    CACTGCTCTG AGGGTGGAGA GGTGACCATC ACCCCTGAAT ACATTTCCGC TACTGATGCA
    GACAGCGATG ACTTAGAACT GCTGTTTCTG ATTGCCCGAG AGCCTCAGCA TGGAGTGGTG
    AGAAAGGCTG GACTTCACGT GGACAGGTTC TCTCAGGGAG ATGTCATCTC AGGGGCTGTG
    ACATACAAAC ATACAGGTGG CGAGATTGGC CTTGAACCTT GTTCTGACAC TGTTGTATTG
    GTGGTTTCGG ATGGTGAAGC TGACCCTTTG ATGAACGGCT GTTGCTACGA TGGACCTGAT
    TCATCTGTTC CTCTTCATAA GTCCTTCCCC ACATACCAGC TCAACATCAC AGTACATCCA
    GTCGACAACC AGCCACCTTC AATTATAATA GGTAGGATGC TCACAGTGGA TGAAGGCTTC
    TCAGCAGCCC TTACTACTCA TCACTTGACT GTTGTTGATT GGGACACTGC CCCGGATGAC
    TTAAAATTCA TGTTGGCTTC TCAGCCCCAG TTTGGCTACT TAGAAAACGC ACTGCCTTCT
    GCAGGCTTTG AGAAAAGTAA CATCGGCATA AGGATAGCCT CATTCCAGTG GACAGACATG
    AAGGCTTCAC ACATTAACTA TGTGCAGTCC AGGCACCTGA GAGTGGAACC AACTGCTGAC
    CAGTTCACAG TATATGCCAC AGACGGGAAG CATCGCTCCT TGGAGACAAC ATTTCACGTT
    ATCATCAACC CCACAAACGA TGAAGCTCCT GACCTTGCAG TACAGAACAT CACTGTGTAT
    GAGGGGCACA TGGTAGAACT GGATTCTTCC ATCATCAGTG CTACTGACAG GGATATTCCC
    AAGGACCCCT TGCTCTTCAG CATCGCTCTC AAGCCCCAGC ACGGCCTGCT CGTCGATGCT
    GCCATCAGCA AGGACTCCCA TCAGATCAAG CAGCTGCAAC ACGAAATCCA CAGCTTCTCC
    GTGGACCTTC TCAAGAATGG AATGAAGCTG GTCTATGCCC ACGACGACTC AGAGAGCTCT
    GCTGATAACT TCGTGATCCA GTTGTCAGAC GGGAAACATA AAATCCTTAA AACCATCTCA
    GTAAACATTA CCCCAGTGAA TGACGAGACA CCAACGCTAA GCAAGAAGGC TGAAATCTCA
    ATGGCTGTGG GCGATACTCG CGTCCTGTCT AGTGCTGTTC TTTCAGCCAC AGATAAAGAC
    TCGCCCAGAG AGAAGATTCA CTATGTATTT GAAAGACTTC CCCAAAATGG ACAACTTCAA
    CTTAAGATAG GGAGGGACTG GGTCCCTCTC TCCACTGGCA TGCAGTGCAC CCAGGAGGAT
    GTGGACCTGA ACTTGCTAAG ATACACGCAT GCTGGTAAAA CGGACTCCCA GGATGGAGAC
    AGCTTCACCT TCTACCTCTG GGATGAGGAC AACAGGTCAC CTGCATTTGA CTGTCACATC
    ATCATTGAAG ACATGGGCAA AGGTGATATT GTCATTCATG CAAAACCACT CGTGGTAGTC
    AAAGGTGACA GAGGTCTCTT GACGACTGCC ACCCTTCTGG CTGTGGATGG AGCAGACAAG
    CCTGAGGAAC TGCTCTACCT CATCACCTCC CCACCACGGC ATGGCCAAGT GGAGTACGTC
    CACTCTCCTG GAGTCCCCAT TGCCAGCTTC AGCCAAATGG ACATAGCAGG ACAGACAGTG
    TGCTACATAC ACAAGAGCAG GACAGCTGTC CCCACTGACA GCTTCAGATT TACCATCAGC
    AATGGACTGC AGACCCAGCG TGGGGTGTTT GAAATCACAC TGCAGACTGT GGACAGCGCC
    TTGCCTGTGC TGACCAAGAA CAAAAGGCTG AGGCTAGCCG AAGGGGCCAT GGGCCTCCTG
    TCCGCTGATC ACCTTCAGCT GACTGACCCC GACACACCTC CAGAGAACCT GACCTTCTTT
    CTGGCTCAGC TCCCACGCCA CGGGTACCTC TTCCTGAGAG GGAAAGCACT CCAACACAAC
    TTCACCCAGC GAGATGTGGA CAGCGGGGGC GTGGCCTACC AGCACTCAGG AGGCGGCGCC
    CGGGAGGACT ATTTTACTTT TCTAGCCACA GACAGGAAGA ACCAAGGCTT TGTTGTGGAT
    GGGAAAGTTC AGAAAGAGCC TGTTCGCTTC ACAATCCAGG TGGATCAGCT GGACAAGGCA
    GCGCCCCGTA TCACACACTT GCACTCCCCT ACTCAAGTGG GGCTCTTGAA AAATGGCTGC
    TATGGGATTT ACATCACTTC CCGTGTGCTG AAGGCATCAG ACCCTGACAC AGAGGATGAC
    CAGATCATCT TTAAGATTTT ACGAGGCCCA TTGTACGGAC GTCTGGAGAA CACAACAACA
    GGTGAATTCA TCCACGAGCG ATTTAGCCAG AAGGACTTAA GCCATAAGAC CATCCTTTAC
    ATCATAAACC CTTCTTTGCA AGTGACTTCC GATATCCTGG AATTTCAGGC CATGGACCCC
    ACAGGGAACA CTGCCACTCC CCAGAGCTTG GACTTGAGAT GGTCGTATAT TGAATGGGCC
    CAGACTGCAT ATGAGGTTTG TGAGAATGTG GGCTTATTGC CCTTGGAAGT TACCAGAAGG
    GGATATCCCA TGGACTCAGC CTTTGTGGGT GTAGAGGTTA ACCAAGTGTC AGCTACAGTT
    GGAAAAGATT TTACTGTCAC TCCATCCAAA CTGCTTCAAT TTGACCCAGG AATGTCAACT
    AAGATGTGGA ATATAGCAAT TACCTATGAC GGATTAGAGG AAGATGATGA GGTCTTTGAA
    GTAATTCTGA ACTCCCCTGT GAATGCGGTT CTTGGCACGC AGACAAAAGC GGCGGTGAAA
    ATTTTGGACT CAAAAGGAGG ACGGTGCCAT CCTTCAAATT CCTTCAACCA AAGCAAGCAC
    AGCACGTGGG GGAAGGGCCC TTGGCATCCG CTGCCCTCAG GCTCATCCTC GCTCACCACT
    TCAGGTTCTC CTCTTCTGGA AAGGCCTCCT CCATCTTTCA CCAGTGGAGA TGCTCTTCAG
    GGTTTTGGTC TCACCGACCT CACTCAAAGG AAGACAATGA CCCAGGGGAA TGGCAAATCA
    GTTCTCCCAT CCTCTGTTTG TAGAAATGGG ACAGACACCA TCTACAATTA CCATGGTATA
    GTTTCCTTGA AACTAGAGGG CGACAGATTC TCAGCTCACA AACGGAAGGC CAAAATATCC
    ATTGTTAGTC AGCCACAGAG GACAATCAAG GTGGCAGAGC TGCCTCTAGC TGATAAGGTG
    GAATCCACAA CTGATTTGCA CTTTCTCAGA CAGGGTCTGC GGCCCTTGTT TCCAAAGAAT
    TGTTCTGTGG ACTTAAAGGG ACTCTTTCAT TTTGAAGAAA GCACCCACAG ATTGTATCAA
    TGTGATGGGA TCTCCTGGAA AGCCTGGAGC CCCCAAACCA AGGGGCTGGA GGACAGATCC
    TGCCCAGGTG GATGGCTCCT TCATTCTGGC TACTGTCACA TCTTGGTCAC AAGACAGAAA
    GGCACCTGGA CCACAGCTAC CCGAGCTTGC AGAGAACAGC ACCAGGGCGA CCTTGTGACC
    GTACTCTCCA GAAGGCATAT GCAGTGGCTA TGGGCGATGA GTGGAAGAAA ACCCTTCTGG
    ATAGGCTTGA AGAACCAACC GAGGACTGGC CACTGGGAGT GGATCGGAGG TGAACCTGTT
    GCTTTTACCA ACTGGAGGAG AGGGGCCCCT CTGCACCCAA AACCTGGAAA GAACTGTGCT
    TTGGTTCAGA AAAGAGGACA ATGGCAGACA AAGAACTGTA GCAAAGGCAA GGCTCACAAT
    TTTGTGTGCT CAAGGAAACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109476.1
    CDS156..5759
    Translation

    Target ORF information:

    RefSeq Version XM_030253616.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253616.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCCATGT TTGTTCTGGA GGTGGACCAG TTCATACTGA CCCCTCTGAC AACCTCCGTT 
    CTGGACTGTG AGGAGGATGA GACTCCGAAA CCCTTGTTGG TGTTCAACAT CACTAAAGCC
    CCACTCCAGG GCTATGTGAC CCACCTGCTG GATCATACCA GACCCATCTC TTCATTCACC
    TGGAAGGATC TCAGTGACAT GCAAGTTGCC TACCAGCCAC CAAACAGCAG CCACCCTGAG
    AGGAGACATT ACACGATGGA GCTGGAGGTG TACGACTTCT TCTTTGAGAG GAGTGCCCCC
    ATCACTGTCC ACATCTCCAT CAGAACAGCA GACACGAATG CACCACGGGT GTCTTGGAAT
    ACAGGTTTGA ATCTCCTAGA GGGGCAGTCT CGGGCCATCA CTTGGGAACA ATTTCAGATC
    GTTGACAATG ATGACATTGG TGCTGTGCAG TTGGTCACCA TTGGTGGCCT GCAGCATGGA
    CGGCTTACAG TACGAGAGGG GAAAGGATTT CTCTTCACCG TGACTGACCT TCAAGCTGGA
    GTTGTCCGCT ATCATCACGA TGACAGTGAT ACCACCAAAG ACTTTGTGGC TTTCAGGATA
    TTCGATGGCC ACCACAGCAG CCACCACAAG TTTCCTATCA ACATCTTGCC TAAAGATGAC
    AGCCCCCCAT TCCTCATCAC CAATGTCGTG ATTGAACTAG AAGAGGGGAA GACCATCTTG
    ATCCAAGGGT CTATGTTGAG AGCTTCAGAT ATGGACTCCA GTGATGACTA CATCTTCTTT
    AATATCACAA AGTTTCCACA GGCTGGGGAG ATCATGAAGA AGCCAGGGCC AAGACTGATA
    GGGTATCCTG TCCCTGGCTT CCTGCAGAGA GACCTGTTCA GTGGAATCAT TTACTATCGT
    CACTTTGGTG GAGAAATCTT TGAAGATTCC TTTGAATTCG TACTGTGGGA CAGCCATGAG
    CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATTCATA TCACTCCAGT AGATGACCAG
    CTTCCTAAAG AAGCTCCTGG CATTTCCCGG CATTTGGTTG TCAAGGAAAC AGAGGTGGCC
    TACATCACTA AAAAGCACCT ACATTTCCTA GACATGGAAT CCCGTGATGG GGAACTCATC
    TACACAGTAA CACGCCCTCC ATGTTTCTCC TTCAGCCACA GACACTTGGA TGCTGGGAAG
    CTATTCATGG TGGACAGCAT ACCAAAGCTA ACCAAAAATC CGACAGCCCC AGGGCTAAGT
    TCATTTACTC AGCATGCCGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT
    ATAGGCCCCT CCCCTCGACA TGTGCAGTTC ACAGTGTCTG TCAGTAACCA GCATGGAGGT
    GCTTTGCATG GAATCTGCTT CAACATCACA GTTCTCCCAG TGGACAACCA GGTTCCCGAG
    GTATTCACCA ACACCCTGAG AGTGGTCGAG GGCGGTCAGT GCACCATCAG CACAGAGCAC
    ATTTTGGTTT CTGATGTGGA CACCCCGCTA GACAGCATCA GCCTCTCTCT TAAGGAGAGG
    CCTCTGCATG GCGGGGTAGA GCTGGACGGA TTTCCTCTAA ACCCAAGAGG CACATTTTCT
    TGGCGAGACC TCAATACCTT AAAAGTTTGG TATCAACATG ATGGATCTGA GGTTCTTCAG
    GATGAGATAT TTTTGGAGGT CACAGATGGC ACAAATTCAG CAGCATTTGT TCTCCACATT
    GAGGTGTTCC CTGTGAATGA TGAGCCACCC ATTCTGAAGG CTGACCTCAT CCCCATGATG
    CACTGCTCTG AGGGTGGAGA GGTGACCATC ACCCCTGAAT ACATTTCCGC TACTGATGCA
    GACAGCGATG ACTTAGAACT GCTGTTTCTG ATTGCCCGAG AGCCTCAGCA TGGAGTGGTG
    AGAAAGGCTG GACTTCACGT GGACAGGTTC TCTCAGGGAG ATGTCATCTC AGGGGCTGTG
    ACATACAAAC ATACAGGTGG CGAGATTGGC CTTGAACCTT GTTCTGACAC TGTTGTATTG
    GTGGTTTCGG ATGGTGAAGC TGACCCTTTG ATGAACGGCT GTTGCTACGA TGGACCTGAT
    TCATCTGTTC CTCTTCATAA GTCCTTCCCC ACATACCAGC TCAACATCAC AGTACATCCA
    GTCGACAACC AGCCACCTTC AATTATAATA GGTAGGATGC TCACAGTGGA TGAAGGCTTC
    TCAGCAGCCC TTACTACTCA TCACTTGACT GTTGTTGATT GGGACACTGC CCCGGATGAC
    TTAAAATTCA TGTTGGCTTC TCAGCCCCAG TTTGGCTACT TAGAAAACGC ACTGCCTTCT
    GCAGGCTTTG AGAAAAGTAA CATCGGCATA AGGATAGCCT CATTCCAGTG GACAGACATG
    AAGGCTTCAC ACATTAACTA TGTGCAGTCC AGGCACCTGA GAGTGGAACC AACTGCTGAC
    CAGTTCACAG TATATGCCAC AGACGGGAAG CATCGCTCCT TGGAGACAAC ATTTCACGTT
    ATCATCAACC CCACAAACGA TGAAGCTCCT GACCTTGCAG TACAGAACAT CACTGTGTAT
    GAGGGGCACA TGGTAGAACT GGATTCTTCC ATCATCAGTG CTACTGACAG GGATATTCCC
    AAGGACCCCT TGCTCTTCAG CATCGCTCTC AAGCCCCAGC ACGGCCTGCT CGTCGATGCT
    GCCATCAGCA AGGACTCCCA TCAGATCAAG CAGCTGCAAC ACGAAATCCA CAGCTTCTCC
    GTGGACCTTC TCAAGAATGG AATGAAGCTG GTCTATGCCC ACGACGACTC AGAGAGCTCT
    GCTGATAACT TCGTGATCCA GTTGTCAGAC GGGAAACATA AAATCCTTAA AACCATCTCA
    GTAAACATTA CCCCAGTGAA TGACGAGACA CCAACGCTAA GCAAGAAGGC TGAAATCTCA
    ATGGCTGTGG GCGATACTCG CGTCCTGTCT AGTGCTGTTC TTTCAGCCAC AGATAAAGAC
    TCGCCCAGAG AGAAGATTCA CTATGTATTT GAAAGACTTC CCCAAAATGG ACAACTTCAA
    CTTAAGATAG GGAGGGACTG GGTCCCTCTC TCCACTGGCA TGCAGTGCAC CCAGGAGGAT
    GTGGACCTGA ACTTGCTAAG ATACACGCAT GCTGGTAAAA CGGACTCCCA GGATGGAGAC
    AGCTTCACCT TCTACCTCTG GGATGAGGAC AACAGGTCAC CTGCATTTGA CTGTCACATC
    ATCATTGAAG ACATGGGCAA AGGTGATATT GTCATTCATG CAAAACCACT CGTGGTAGTC
    AAAGGTGACA GAGGTCTCTT GACGACTGCC ACCCTTCTGG CTGTGGATGG AGCAGACAAG
    CCTGAGGAAC TGCTCTACCT CATCACCTCC CCACCACGGC ATGGCCAAGT GGAGTACGTC
    CACTCTCCTG GAGTCCCCAT TGCCAGCTTC AGCCAAATGG ACATAGCAGG ACAGACAGTG
    TGCTACATAC ACAAGAGCAG GACAGCTGTC CCCACTGACA GCTTCAGATT TACCATCAGC
    AATGGACTGC AGACCCAGCG TGGGGTGTTT GAAATCACAC TGCAGACTGT GGACAGCGCC
    TTGCCTGTGC TGACCAAGAA CAAAAGGCTG AGGCTAGCCG AAGGGGCCAT GGGCCTCCTG
    TCCGCTGATC ACCTTCAGCT GACTGACCCC GACACACCTC CAGAGAACCT GACCTTCTTT
    CTGGCTCAGC TCCCACGCCA CGGGTACCTC TTCCTGAGAG GGAAAGCACT CCAACACAAC
    TTCACCCAGC GAGATGTGGA CAGCGGGGGC GTGGCCTACC AGCACTCAGG AGGCGGCGCC
    CGGGAGGACT ATTTTACTTT TCTAGCCACA GACAGGAAGA ACCAAGGCTT TGTTGTGGAT
    GGGAAAGTTC AGAAAGAGCC TGTTCGCTTC ACAATCCAGG TGGATCAGCT GGACAAGGCA
    GCGCCCCGTA TCACACACTT GCACTCCCCT ACTCAAGTGG GGCTCTTGAA AAATGGCTGC
    TATGGGATTT ACATCACTTC CCGTGTGCTG AAGGCATCAG ACCCTGACAC AGAGGATGAC
    CAGATCATCT TTAAGATTTT ACGAGGCCCA TTGTACGGAC GTCTGGAGAA CACAACAACA
    GGTGAATTCA TCCACGAGCG ATTTAGCCAG AAGGACTTAA GCCATAAGAC CATCCTTTAC
    ATCATAAACC CTTCTTTGCA AGTGACTTCC GATATCCTGG AATTTCAGGC CATGGACCCC
    ACAGGGAACA CTGCCACTCC CCAGAGCTTG GACTTGAGAT GGTCGTATAT TGAATGGGCC
    CAGACTGCAT ATGAGGTTTG TGAGAATGTG GGCTTATTGC CCTTGGAAGT TACCAGAAGG
    GGATATCCCA TGGACTCAGC CTTTGTGGGT GTAGAGGTTA ACCAAGTGTC AGCTACAGTT
    GGAAAAGATT TTACTGTCAC TCCATCCAAA CTGCTTCAAT TTGACCCAGG AATGTCAACT
    AAGATGTGGA ATATAGCAAT TACCTATGAC GGATTAGAGG AAGATGATGA GGTCTTTGAA
    GTAATTCTGA ACTCCCCTGT GAATGCGGTT CTTGGCACGC AGACAAAAGC GGCGGTGAAA
    ATTTTGGACT CAAAAGGAGG ACGGTGCCAT CCTTCAAATT CCTTCAACCA AAGCAAGCAC
    AGCACGTGGG GGAAGGGCCC TTGGCATCCG CTGCCCTCAG GCTCATCCTC GCTCACCACT
    TCAGGTTCTC CTCTTCTGGA AAGGCCTCCT CCATCTTTCA CCAGTGGAGA TGCTCTTCAG
    GGTTTTGGTC TCACCGACCT CACTCAAAGG AAGACAATGA CCCAGGGGAA TGGCAAATCA
    GTTCTCCCAT CCTCTGTTTG TAGAAATGGG ACAGACACCA TCTACAATTA CCATGGTATA
    GTTTCCTTGA AACTAGAGGG CGACAGATTC TCAGCTCACA AACGGAAGGC CAAAATATCC
    ATTGTTAGTC AGCCACAGAG GACAATCAAG GTGGCAGAGC TGCCTCTAGC TGATAAGGTG
    GAATCCACAA CTGATTTGCA CTTTCTCAGA CAGGGTCTGC GGCCCTTGTT TCCAAAGAAT
    TGTTCTGTGG ACTTAAAGGG ACTCTTTCAT TTTGAAGAAA GCACCCACAG ATTGTATCAA
    TGTGATGGGA TCTCCTGGAA AGCCTGGAGC CCCCAAACCA AGGGGCTGGA GGACAGATCC
    TGCCCAGGTG GATGGCTCCT TCATTCTGGC TACTGTCACA TCTTGGTCAC AAGACAGAAA
    GGCACCTGGA CCACAGCTAC CCGAGCTTGC AGAGAACAGC ACCAGGGCGA CCTTGTGACC
    GTACTCTCCA GAAGGCATAT GCAGTGGCTA TGGGCGATGA GTGGAAGAAA ACCCTTCTGG
    ATAGGCTTGA AGAACCAACC GAGGACTGGC CACTGGGAGT GGATCGGAGG TGAACCTGTT
    GCTTTTACCA ACTGGAGGAG AGGGGCCCCT CTGCACCCAA AACCTGGAAA GAACTGTGCT
    TTGGTTCAGA AAAGAGGACA ATGGCAGACA AAGAACTGTA GCAAAGGCAA GGCTCACAAT
    TTTGTGTGCT CAAGGAAACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu32989
    Clone ID Related Accession (Same CDS sequence) XM_030253616.1 , XM_006538017.3
    Accession Version XM_006538017.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCCATGT TTGTTCTGGA GGTGGACCAG TTCATACTGA CCCCTCTGAC AACCTCCGTT 
    CTGGACTGTG AGGAGGATGA GACTCCGAAA CCCTTGTTGG TGTTCAACAT CACTAAAGCC
    CCACTCCAGG GCTATGTGAC CCACCTGCTG GATCATACCA GACCCATCTC TTCATTCACC
    TGGAAGGATC TCAGTGACAT GCAAGTTGCC TACCAGCCAC CAAACAGCAG CCACCCTGAG
    AGGAGACATT ACACGATGGA GCTGGAGGTG TACGACTTCT TCTTTGAGAG GAGTGCCCCC
    ATCACTGTCC ACATCTCCAT CAGAACAGCA GACACGAATG CACCACGGGT GTCTTGGAAT
    ACAGGTTTGA ATCTCCTAGA GGGGCAGTCT CGGGCCATCA CTTGGGAACA ATTTCAGATC
    GTTGACAATG ATGACATTGG TGCTGTGCAG TTGGTCACCA TTGGTGGCCT GCAGCATGGA
    CGGCTTACAG TACGAGAGGG GAAAGGATTT CTCTTCACCG TGACTGACCT TCAAGCTGGA
    GTTGTCCGCT ATCATCACGA TGACAGTGAT ACCACCAAAG ACTTTGTGGC TTTCAGGATA
    TTCGATGGCC ACCACAGCAG CCACCACAAG TTTCCTATCA ACATCTTGCC TAAAGATGAC
    AGCCCCCCAT TCCTCATCAC CAATGTCGTG ATTGAACTAG AAGAGGGGAA GACCATCTTG
    ATCCAAGGGT CTATGTTGAG AGCTTCAGAT ATGGACTCCA GTGATGACTA CATCTTCTTT
    AATATCACAA AGTTTCCACA GGCTGGGGAG ATCATGAAGA AGCCAGGGCC AAGACTGATA
    GGGTATCCTG TCCCTGGCTT CCTGCAGAGA GACCTGTTCA GTGGAATCAT TTACTATCGT
    CACTTTGGTG GAGAAATCTT TGAAGATTCC TTTGAATTCG TACTGTGGGA CAGCCATGAG
    CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATTCATA TCACTCCAGT AGATGACCAG
    CTTCCTAAAG AAGCTCCTGG CATTTCCCGG CATTTGGTTG TCAAGGAAAC AGAGGTGGCC
    TACATCACTA AAAAGCACCT ACATTTCCTA GACATGGAAT CCCGTGATGG GGAACTCATC
    TACACAGTAA CACGCCCTCC ATGTTTCTCC TTCAGCCACA GACACTTGGA TGCTGGGAAG
    CTATTCATGG TGGACAGCAT ACCAAAGCTA ACCAAAAATC CGACAGCCCC AGGGCTAAGT
    TCATTTACTC AGCATGCCGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT
    ATAGGCCCCT CCCCTCGACA TGTGCAGTTC ACAGTGTCTG TCAGTAACCA GCATGGAGGT
    GCTTTGCATG GAATCTGCTT CAACATCACA GTTCTCCCAG TGGACAACCA GGTTCCCGAG
    GTATTCACCA ACACCCTGAG AGTGGTCGAG GGCGGTCAGT GCACCATCAG CACAGAGCAC
    ATTTTGGTTT CTGATGTGGA CACCCCGCTA GACAGCATCA GCCTCTCTCT TAAGGAGAGG
    CCTCTGCATG GCGGGGTAGA GCTGGACGGA TTTCCTCTAA ACCCAAGAGG CACATTTTCT
    TGGCGAGACC TCAATACCTT AAAAGTTTGG TATCAACATG ATGGATCTGA GGTTCTTCAG
    GATGAGATAT TTTTGGAGGT CACAGATGGC ACAAATTCAG CAGCATTTGT TCTCCACATT
    GAGGTGTTCC CTGTGAATGA TGAGCCACCC ATTCTGAAGG CTGACCTCAT CCCCATGATG
    CACTGCTCTG AGGGTGGAGA GGTGACCATC ACCCCTGAAT ACATTTCCGC TACTGATGCA
    GACAGCGATG ACTTAGAACT GCTGTTTCTG ATTGCCCGAG AGCCTCAGCA TGGAGTGGTG
    AGAAAGGCTG GACTTCACGT GGACAGGTTC TCTCAGGGAG ATGTCATCTC AGGGGCTGTG
    ACATACAAAC ATACAGGTGG CGAGATTGGC CTTGAACCTT GTTCTGACAC TGTTGTATTG
    GTGGTTTCGG ATGGTGAAGC TGACCCTTTG ATGAACGGCT GTTGCTACGA TGGACCTGAT
    TCATCTGTTC CTCTTCATAA GTCCTTCCCC ACATACCAGC TCAACATCAC AGTACATCCA
    GTCGACAACC AGCCACCTTC AATTATAATA GGTAGGATGC TCACAGTGGA TGAAGGCTTC
    TCAGCAGCCC TTACTACTCA TCACTTGACT GTTGTTGATT GGGACACTGC CCCGGATGAC
    TTAAAATTCA TGTTGGCTTC TCAGCCCCAG TTTGGCTACT TAGAAAACGC ACTGCCTTCT
    GCAGGCTTTG AGAAAAGTAA CATCGGCATA AGGATAGCCT CATTCCAGTG GACAGACATG
    AAGGCTTCAC ACATTAACTA TGTGCAGTCC AGGCACCTGA GAGTGGAACC AACTGCTGAC
    CAGTTCACAG TATATGCCAC AGACGGGAAG CATCGCTCCT TGGAGACAAC ATTTCACGTT
    ATCATCAACC CCACAAACGA TGAAGCTCCT GACCTTGCAG TACAGAACAT CACTGTGTAT
    GAGGGGCACA TGGTAGAACT GGATTCTTCC ATCATCAGTG CTACTGACAG GGATATTCCC
    AAGGACCCCT TGCTCTTCAG CATCGCTCTC AAGCCCCAGC ACGGCCTGCT CGTCGATGCT
    GCCATCAGCA AGGACTCCCA TCAGATCAAG CAGCTGCAAC ACGAAATCCA CAGCTTCTCC
    GTGGACCTTC TCAAGAATGG AATGAAGCTG GTCTATGCCC ACGACGACTC AGAGAGCTCT
    GCTGATAACT TCGTGATCCA GTTGTCAGAC GGGAAACATA AAATCCTTAA AACCATCTCA
    GTAAACATTA CCCCAGTGAA TGACGAGACA CCAACGCTAA GCAAGAAGGC TGAAATCTCA
    ATGGCTGTGG GCGATACTCG CGTCCTGTCT AGTGCTGTTC TTTCAGCCAC AGATAAAGAC
    TCGCCCAGAG AGAAGATTCA CTATGTATTT GAAAGACTTC CCCAAAATGG ACAACTTCAA
    CTTAAGATAG GGAGGGACTG GGTCCCTCTC TCCACTGGCA TGCAGTGCAC CCAGGAGGAT
    GTGGACCTGA ACTTGCTAAG ATACACGCAT GCTGGTAAAA CGGACTCCCA GGATGGAGAC
    AGCTTCACCT TCTACCTCTG GGATGAGGAC AACAGGTCAC CTGCATTTGA CTGTCACATC
    ATCATTGAAG ACATGGGCAA AGGTGATATT GTCATTCATG CAAAACCACT CGTGGTAGTC
    AAAGGTGACA GAGGTCTCTT GACGACTGCC ACCCTTCTGG CTGTGGATGG AGCAGACAAG
    CCTGAGGAAC TGCTCTACCT CATCACCTCC CCACCACGGC ATGGCCAAGT GGAGTACGTC
    CACTCTCCTG GAGTCCCCAT TGCCAGCTTC AGCCAAATGG ACATAGCAGG ACAGACAGTG
    TGCTACATAC ACAAGAGCAG GACAGCTGTC CCCACTGACA GCTTCAGATT TACCATCAGC
    AATGGACTGC AGACCCAGCG TGGGGTGTTT GAAATCACAC TGCAGACTGT GGACAGCGCC
    TTGCCTGTGC TGACCAAGAA CAAAAGGCTG AGGCTAGCCG AAGGGGCCAT GGGCCTCCTG
    TCCGCTGATC ACCTTCAGCT GACTGACCCC GACACACCTC CAGAGAACCT GACCTTCTTT
    CTGGCTCAGC TCCCACGCCA CGGGTACCTC TTCCTGAGAG GGAAAGCACT CCAACACAAC
    TTCACCCAGC GAGATGTGGA CAGCGGGGGC GTGGCCTACC AGCACTCAGG AGGCGGCGCC
    CGGGAGGACT ATTTTACTTT TCTAGCCACA GACAGGAAGA ACCAAGGCTT TGTTGTGGAT
    GGGAAAGTTC AGAAAGAGCC TGTTCGCTTC ACAATCCAGG TGGATCAGCT GGACAAGGCA
    GCGCCCCGTA TCACACACTT GCACTCCCCT ACTCAAGTGG GGCTCTTGAA AAATGGCTGC
    TATGGGATTT ACATCACTTC CCGTGTGCTG AAGGCATCAG ACCCTGACAC AGAGGATGAC
    CAGATCATCT TTAAGATTTT ACGAGGCCCA TTGTACGGAC GTCTGGAGAA CACAACAACA
    GGTGAATTCA TCCACGAGCG ATTTAGCCAG AAGGACTTAA GCCATAAGAC CATCCTTTAC
    ATCATAAACC CTTCTTTGCA AGTGACTTCC GATATCCTGG AATTTCAGGC CATGGACCCC
    ACAGGGAACA CTGCCACTCC CCAGAGCTTG GACTTGAGAT GGTCGTATAT TGAATGGGCC
    CAGACTGCAT ATGAGGTTTG TGAGAATGTG GGCTTATTGC CCTTGGAAGT TACCAGAAGG
    GGATATCCCA TGGACTCAGC CTTTGTGGGT GTAGAGGTTA ACCAAGTGTC AGCTACAGTT
    GGAAAAGATT TTACTGTCAC TCCATCCAAA CTGCTTCAAT TTGACCCAGG AATGTCAACT
    AAGATGTGGA ATATAGCAAT TACCTATGAC GGATTAGAGG AAGATGATGA GGTCTTTGAA
    GTAATTCTGA ACTCCCCTGT GAATGCGGTT CTTGGCACGC AGACAAAAGC GGCGGTGAAA
    ATTTTGGACT CAAAAGGAGG ACGGTGCCAT CCTTCAAATT CCTTCAACCA AAGCAAGCAC
    AGCACGTGGG GGAAGGGCCC TTGGCATCCG CTGCCCTCAG GCTCATCCTC GCTCACCACT
    TCAGGTTCTC CTCTTCTGGA AAGGCCTCCT CCATCTTTCA CCAGTGGAGA TGCTCTTCAG
    GGTTTTGGTC TCACCGACCT CACTCAAAGG AAGACAATGA CCCAGGGGAA TGGCAAATCA
    GTTCTCCCAT CCTCTGTTTG TAGAAATGGG ACAGACACCA TCTACAATTA CCATGGTATA
    GTTTCCTTGA AACTAGAGGG CGACAGATTC TCAGCTCACA AACGGAAGGC CAAAATATCC
    ATTGTTAGTC AGCCACAGAG GACAATCAAG GTGGCAGAGC TGCCTCTAGC TGATAAGGTG
    GAATCCACAA CTGATTTGCA CTTTCTCAGA CAGGGTCTGC GGCCCTTGTT TCCAAAGAAT
    TGTTCTGTGG ACTTAAAGGG ACTCTTTCAT TTTGAAGAAA GCACCCACAG ATTGTATCAA
    TGTGATGGGA TCTCCTGGAA AGCCTGGAGC CCCCAAACCA AGGGGCTGGA GGACAGATCC
    TGCCCAGGTG GATGGCTCCT TCATTCTGGC TACTGTCACA TCTTGGTCAC AAGACAGAAA
    GGCACCTGGA CCACAGCTAC CCGAGCTTGC AGAGAACAGC ACCAGGGCGA CCTTGTGACC
    GTACTCTCCA GAAGGCATAT GCAGTGGCTA TGGGCGATGA GTGGAAGAAA ACCCTTCTGG
    ATAGGCTTGA AGAACCAACC GAGGACTGGC CACTGGGAGT GGATCGGAGG TGAACCTGTT
    GCTTTTACCA ACTGGAGGAG AGGGGCCCCT CTGCACCCAA AACCTGGAAA GAACTGTGCT
    TTGGTTCAGA AAAGAGGACA ATGGCAGACA AAGAACTGTA GCAAAGGCAA GGCTCACAAT
    TTTGTGTGCT CAAGGAAACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006538080.1
    CDS1670..7273
    Translation

    Target ORF information:

    RefSeq Version XM_006538017.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006538017.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCCATGT TTGTTCTGGA GGTGGACCAG TTCATACTGA CCCCTCTGAC AACCTCCGTT 
    CTGGACTGTG AGGAGGATGA GACTCCGAAA CCCTTGTTGG TGTTCAACAT CACTAAAGCC
    CCACTCCAGG GCTATGTGAC CCACCTGCTG GATCATACCA GACCCATCTC TTCATTCACC
    TGGAAGGATC TCAGTGACAT GCAAGTTGCC TACCAGCCAC CAAACAGCAG CCACCCTGAG
    AGGAGACATT ACACGATGGA GCTGGAGGTG TACGACTTCT TCTTTGAGAG GAGTGCCCCC
    ATCACTGTCC ACATCTCCAT CAGAACAGCA GACACGAATG CACCACGGGT GTCTTGGAAT
    ACAGGTTTGA ATCTCCTAGA GGGGCAGTCT CGGGCCATCA CTTGGGAACA ATTTCAGATC
    GTTGACAATG ATGACATTGG TGCTGTGCAG TTGGTCACCA TTGGTGGCCT GCAGCATGGA
    CGGCTTACAG TACGAGAGGG GAAAGGATTT CTCTTCACCG TGACTGACCT TCAAGCTGGA
    GTTGTCCGCT ATCATCACGA TGACAGTGAT ACCACCAAAG ACTTTGTGGC TTTCAGGATA
    TTCGATGGCC ACCACAGCAG CCACCACAAG TTTCCTATCA ACATCTTGCC TAAAGATGAC
    AGCCCCCCAT TCCTCATCAC CAATGTCGTG ATTGAACTAG AAGAGGGGAA GACCATCTTG
    ATCCAAGGGT CTATGTTGAG AGCTTCAGAT ATGGACTCCA GTGATGACTA CATCTTCTTT
    AATATCACAA AGTTTCCACA GGCTGGGGAG ATCATGAAGA AGCCAGGGCC AAGACTGATA
    GGGTATCCTG TCCCTGGCTT CCTGCAGAGA GACCTGTTCA GTGGAATCAT TTACTATCGT
    CACTTTGGTG GAGAAATCTT TGAAGATTCC TTTGAATTCG TACTGTGGGA CAGCCATGAG
    CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATTCATA TCACTCCAGT AGATGACCAG
    CTTCCTAAAG AAGCTCCTGG CATTTCCCGG CATTTGGTTG TCAAGGAAAC AGAGGTGGCC
    TACATCACTA AAAAGCACCT ACATTTCCTA GACATGGAAT CCCGTGATGG GGAACTCATC
    TACACAGTAA CACGCCCTCC ATGTTTCTCC TTCAGCCACA GACACTTGGA TGCTGGGAAG
    CTATTCATGG TGGACAGCAT ACCAAAGCTA ACCAAAAATC CGACAGCCCC AGGGCTAAGT
    TCATTTACTC AGCATGCCGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT
    ATAGGCCCCT CCCCTCGACA TGTGCAGTTC ACAGTGTCTG TCAGTAACCA GCATGGAGGT
    GCTTTGCATG GAATCTGCTT CAACATCACA GTTCTCCCAG TGGACAACCA GGTTCCCGAG
    GTATTCACCA ACACCCTGAG AGTGGTCGAG GGCGGTCAGT GCACCATCAG CACAGAGCAC
    ATTTTGGTTT CTGATGTGGA CACCCCGCTA GACAGCATCA GCCTCTCTCT TAAGGAGAGG
    CCTCTGCATG GCGGGGTAGA GCTGGACGGA TTTCCTCTAA ACCCAAGAGG CACATTTTCT
    TGGCGAGACC TCAATACCTT AAAAGTTTGG TATCAACATG ATGGATCTGA GGTTCTTCAG
    GATGAGATAT TTTTGGAGGT CACAGATGGC ACAAATTCAG CAGCATTTGT TCTCCACATT
    GAGGTGTTCC CTGTGAATGA TGAGCCACCC ATTCTGAAGG CTGACCTCAT CCCCATGATG
    CACTGCTCTG AGGGTGGAGA GGTGACCATC ACCCCTGAAT ACATTTCCGC TACTGATGCA
    GACAGCGATG ACTTAGAACT GCTGTTTCTG ATTGCCCGAG AGCCTCAGCA TGGAGTGGTG
    AGAAAGGCTG GACTTCACGT GGACAGGTTC TCTCAGGGAG ATGTCATCTC AGGGGCTGTG
    ACATACAAAC ATACAGGTGG CGAGATTGGC CTTGAACCTT GTTCTGACAC TGTTGTATTG
    GTGGTTTCGG ATGGTGAAGC TGACCCTTTG ATGAACGGCT GTTGCTACGA TGGACCTGAT
    TCATCTGTTC CTCTTCATAA GTCCTTCCCC ACATACCAGC TCAACATCAC AGTACATCCA
    GTCGACAACC AGCCACCTTC AATTATAATA GGTAGGATGC TCACAGTGGA TGAAGGCTTC
    TCAGCAGCCC TTACTACTCA TCACTTGACT GTTGTTGATT GGGACACTGC CCCGGATGAC
    TTAAAATTCA TGTTGGCTTC TCAGCCCCAG TTTGGCTACT TAGAAAACGC ACTGCCTTCT
    GCAGGCTTTG AGAAAAGTAA CATCGGCATA AGGATAGCCT CATTCCAGTG GACAGACATG
    AAGGCTTCAC ACATTAACTA TGTGCAGTCC AGGCACCTGA GAGTGGAACC AACTGCTGAC
    CAGTTCACAG TATATGCCAC AGACGGGAAG CATCGCTCCT TGGAGACAAC ATTTCACGTT
    ATCATCAACC CCACAAACGA TGAAGCTCCT GACCTTGCAG TACAGAACAT CACTGTGTAT
    GAGGGGCACA TGGTAGAACT GGATTCTTCC ATCATCAGTG CTACTGACAG GGATATTCCC
    AAGGACCCCT TGCTCTTCAG CATCGCTCTC AAGCCCCAGC ACGGCCTGCT CGTCGATGCT
    GCCATCAGCA AGGACTCCCA TCAGATCAAG CAGCTGCAAC ACGAAATCCA CAGCTTCTCC
    GTGGACCTTC TCAAGAATGG AATGAAGCTG GTCTATGCCC ACGACGACTC AGAGAGCTCT
    GCTGATAACT TCGTGATCCA GTTGTCAGAC GGGAAACATA AAATCCTTAA AACCATCTCA
    GTAAACATTA CCCCAGTGAA TGACGAGACA CCAACGCTAA GCAAGAAGGC TGAAATCTCA
    ATGGCTGTGG GCGATACTCG CGTCCTGTCT AGTGCTGTTC TTTCAGCCAC AGATAAAGAC
    TCGCCCAGAG AGAAGATTCA CTATGTATTT GAAAGACTTC CCCAAAATGG ACAACTTCAA
    CTTAAGATAG GGAGGGACTG GGTCCCTCTC TCCACTGGCA TGCAGTGCAC CCAGGAGGAT
    GTGGACCTGA ACTTGCTAAG ATACACGCAT GCTGGTAAAA CGGACTCCCA GGATGGAGAC
    AGCTTCACCT TCTACCTCTG GGATGAGGAC AACAGGTCAC CTGCATTTGA CTGTCACATC
    ATCATTGAAG ACATGGGCAA AGGTGATATT GTCATTCATG CAAAACCACT CGTGGTAGTC
    AAAGGTGACA GAGGTCTCTT GACGACTGCC ACCCTTCTGG CTGTGGATGG AGCAGACAAG
    CCTGAGGAAC TGCTCTACCT CATCACCTCC CCACCACGGC ATGGCCAAGT GGAGTACGTC
    CACTCTCCTG GAGTCCCCAT TGCCAGCTTC AGCCAAATGG ACATAGCAGG ACAGACAGTG
    TGCTACATAC ACAAGAGCAG GACAGCTGTC CCCACTGACA GCTTCAGATT TACCATCAGC
    AATGGACTGC AGACCCAGCG TGGGGTGTTT GAAATCACAC TGCAGACTGT GGACAGCGCC
    TTGCCTGTGC TGACCAAGAA CAAAAGGCTG AGGCTAGCCG AAGGGGCCAT GGGCCTCCTG
    TCCGCTGATC ACCTTCAGCT GACTGACCCC GACACACCTC CAGAGAACCT GACCTTCTTT
    CTGGCTCAGC TCCCACGCCA CGGGTACCTC TTCCTGAGAG GGAAAGCACT CCAACACAAC
    TTCACCCAGC GAGATGTGGA CAGCGGGGGC GTGGCCTACC AGCACTCAGG AGGCGGCGCC
    CGGGAGGACT ATTTTACTTT TCTAGCCACA GACAGGAAGA ACCAAGGCTT TGTTGTGGAT
    GGGAAAGTTC AGAAAGAGCC TGTTCGCTTC ACAATCCAGG TGGATCAGCT GGACAAGGCA
    GCGCCCCGTA TCACACACTT GCACTCCCCT ACTCAAGTGG GGCTCTTGAA AAATGGCTGC
    TATGGGATTT ACATCACTTC CCGTGTGCTG AAGGCATCAG ACCCTGACAC AGAGGATGAC
    CAGATCATCT TTAAGATTTT ACGAGGCCCA TTGTACGGAC GTCTGGAGAA CACAACAACA
    GGTGAATTCA TCCACGAGCG ATTTAGCCAG AAGGACTTAA GCCATAAGAC CATCCTTTAC
    ATCATAAACC CTTCTTTGCA AGTGACTTCC GATATCCTGG AATTTCAGGC CATGGACCCC
    ACAGGGAACA CTGCCACTCC CCAGAGCTTG GACTTGAGAT GGTCGTATAT TGAATGGGCC
    CAGACTGCAT ATGAGGTTTG TGAGAATGTG GGCTTATTGC CCTTGGAAGT TACCAGAAGG
    GGATATCCCA TGGACTCAGC CTTTGTGGGT GTAGAGGTTA ACCAAGTGTC AGCTACAGTT
    GGAAAAGATT TTACTGTCAC TCCATCCAAA CTGCTTCAAT TTGACCCAGG AATGTCAACT
    AAGATGTGGA ATATAGCAAT TACCTATGAC GGATTAGAGG AAGATGATGA GGTCTTTGAA
    GTAATTCTGA ACTCCCCTGT GAATGCGGTT CTTGGCACGC AGACAAAAGC GGCGGTGAAA
    ATTTTGGACT CAAAAGGAGG ACGGTGCCAT CCTTCAAATT CCTTCAACCA AAGCAAGCAC
    AGCACGTGGG GGAAGGGCCC TTGGCATCCG CTGCCCTCAG GCTCATCCTC GCTCACCACT
    TCAGGTTCTC CTCTTCTGGA AAGGCCTCCT CCATCTTTCA CCAGTGGAGA TGCTCTTCAG
    GGTTTTGGTC TCACCGACCT CACTCAAAGG AAGACAATGA CCCAGGGGAA TGGCAAATCA
    GTTCTCCCAT CCTCTGTTTG TAGAAATGGG ACAGACACCA TCTACAATTA CCATGGTATA
    GTTTCCTTGA AACTAGAGGG CGACAGATTC TCAGCTCACA AACGGAAGGC CAAAATATCC
    ATTGTTAGTC AGCCACAGAG GACAATCAAG GTGGCAGAGC TGCCTCTAGC TGATAAGGTG
    GAATCCACAA CTGATTTGCA CTTTCTCAGA CAGGGTCTGC GGCCCTTGTT TCCAAAGAAT
    TGTTCTGTGG ACTTAAAGGG ACTCTTTCAT TTTGAAGAAA GCACCCACAG ATTGTATCAA
    TGTGATGGGA TCTCCTGGAA AGCCTGGAGC CCCCAAACCA AGGGGCTGGA GGACAGATCC
    TGCCCAGGTG GATGGCTCCT TCATTCTGGC TACTGTCACA TCTTGGTCAC AAGACAGAAA
    GGCACCTGGA CCACAGCTAC CCGAGCTTGC AGAGAACAGC ACCAGGGCGA CCTTGTGACC
    GTACTCTCCA GAAGGCATAT GCAGTGGCTA TGGGCGATGA GTGGAAGAAA ACCCTTCTGG
    ATAGGCTTGA AGAACCAACC GAGGACTGGC CACTGGGAGT GGATCGGAGG TGAACCTGTT
    GCTTTTACCA ACTGGAGGAG AGGGGCCCCT CTGCACCCAA AACCTGGAAA GAACTGTGCT
    TTGGTTCAGA AAAGAGGACA ATGGCAGACA AAGAACTGTA GCAAAGGCAA GGCTCACAAT
    TTTGTGTGCT CAAGGAAACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu32990
    Clone ID Related Accession (Same CDS sequence) XM_006538018.2
    Accession Version XM_006538018.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538018.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACCAGCT TCCTAAAGAA GCTCCTGGCA TTTCCCGGCA TTTGGTTGTC AAGGAAACAG 
    AGACACTTGG ATGCTGGGAA GCTATTCATG GTGGACAGCA TACCAAAGCT AACCAAAAAT
    CCGACAGCCC CAGGGCTAAG TTCATTTACT CAGCATGCCG TGAACCATAT GAAAGTGGCC
    TACATGCCAC CCATGCAAGA TATAGGCCCC TCCCCTCGAC ATGTGCAGTT CACAGTGTCT
    GTCAGTAACC AGCATGGAGG TGCTTTGCAT GGAATCTGCT TCAACATCAC AGTTCTCCCA
    GTGGACAACC AGGTTCCCGA GGTATTCACC AACACCCTGA GAGTGGTCGA GGGCGGTCAG
    TGCACCATCA GCACAGAGCA CATTTTGGTT TCTGATGTGG ACACCCCGCT AGACAGCATC
    AGCCTCTCTC TTAAGGAGAG GCCTCTGCAT GGCGGGGTAG AGCTGGACGG ATTTCCTCTA
    AACCCAAGAG GCACATTTTC TTGGCGAGAC CTCAATACCT TAAAAGTTTG GTATCAACAT
    GATGGATCTG AGGTTCTTCA GGATGAGATA TTTTTGGAGG TCACAGATGG CACAAATTCA
    GCAGCATTTG TTCTCCACAT TGAGGTGTTC CCTGTGAATG ATGAGCCACC CATTCTGAAG
    GCTGACCTCA TCCCCATGAT GCACTGCTCT GAGGGTGGAG AGGTGACCAT CACCCCTGAA
    TACATTTCCG CTACTGATGC AGACAGCGAT GACTTAGAAC TGCTGTTTCT GATTGCCCGA
    GAGCCTCAGC ATGGAGTGGT GAGAAAGGCT GGACTTCACG TGGACAGGTT CTCTCAGGGA
    GATGTCATCT CAGGGGCTGT GACATACAAA CATACAGGTG GCGAGATTGG CCTTGAACCT
    TGTTCTGACA CTGTTGTATT GGTGGTTTCG GATGGTGAAG CTGACCCTTT GATGAACGGC
    TGTTGCTACG ATGGACCTGA TTCATCTGTT CCTCTTCATA AGTCCTTCCC CACATACCAG
    CTCAACATCA CAGTACATCC AGTCGACAAC CAGCCACCTT CAATTATAAT AGGTAGGATG
    CTCACAGTGG ATGAAGGCTT CTCAGCAGCC CTTACTACTC ATCACTTGAC TGTTGTTGAT
    TGGGACACTG CCCCGGATGA CTTAAAATTC ATGTTGGCTT CTCAGCCCCA GTTTGGCTAC
    TTAGAAAACG CACTGCCTTC TGCAGGCTTT GAGAAAAGTA ACATCGGCAT AAGGATAGCC
    TCATTCCAGT GGACAGACAT GAAGGCTTCA CACATTAACT ATGTGCAGTC CAGGCACCTG
    AGAGTGGAAC CAACTGCTGA CCAGTTCACA GTATATGCCA CAGACGGGAA GCATCGCTCC
    TTGGAGACAA CATTTCACGT TATCATCAAC CCCACAAACG ATGAAGCTCC TGACCTTGCA
    GTACAGAACA TCACTGTGTA TGAGGGGCAC ATGGTAGAAC TGGATTCTTC CATCATCAGT
    GCTACTGACA GGGATATTCC CAAGGACCCC TTGCTCTTCA GCATCGCTCT CAAGCCCCAG
    CACGGCCTGC TCGTCGATGC TGCCATCAGC AAGGACTCCC ATCAGATCAA GCAGCTGCAA
    CACGAAATCC ACAGCTTCTC CGTGGACCTT CTCAAGAATG GAATGAAGCT GGTCTATGCC
    CACGACGACT CAGAGAGCTC TGCTGATAAC TTCGTGATCC AGTTGTCAGA CGGGAAACAT
    AAAATCCTTA AAACCATCTC AGTAAACATT ACCCCAGTGA ATGACGAGAC ACCAACGCTA
    AGCAAGAAGG CTGAAATCTC AATGGCTGTG GGCGATACTC GCGTCCTGTC TAGTGCTGTT
    CTTTCAGCCA CAGATAAAGA CTCGCCCAGA GAGAAGATTC ACTATGTATT TGAAAGACTT
    CCCCAAAATG GACAACTTCA ACTTAAGATA GGGAGGGACT GGGTCCCTCT CTCCACTGGC
    ATGCAGTGCA CCCAGGAGGA TGTGGACCTG AACTTGCTAA GATACACGCA TGCTGGTAAA
    ACGGACTCCC AGGATGGAGA CAGCTTCACC TTCTACCTCT GGGATGAGGA CAACAGGTCA
    CCTGCATTTG ACTGTCACAT CATCATTGAA GACATGGGCA AAGGTGATAT TGTCATTCAT
    GCAAAACCAC TCGTGGTAGT CAAAGGTGAC AGAGGTCTCT TGACGACTGC CACCCTTCTG
    GCTGTGGATG GAGCAGACAA GCCTGAGGAA CTGCTCTACC TCATCACCTC CCCACCACGG
    CATGGCCAAG TGGAGTACGT CCACTCTCCT GGAGTCCCCA TTGCCAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT GTGCTACATA CACAAGAGCA GGACAGCTGT CCCCACTGAC
    AGCTTCAGAT TTACCATCAG CAATGGACTG CAGACCCAGC GTGGGGTGTT TGAAATCACA
    CTGCAGACTG TGGACAGCGC CTTGCCTGTG CTGACCAAGA ACAAAAGGCT GAGGCTAGCC
    GAAGGGGCCA TGGGCCTCCT GTCCGCTGAT CACCTTCAGC TGACTGACCC CGACACACCT
    CCAGAGAACC TGACCTTCTT TCTGGCTCAG CTCCCACGCC ACGGGTACCT CTTCCTGAGA
    GGGAAAGCAC TCCAACACAA CTTCACCCAG CGAGATGTGG ACAGCGGGGG CGTGGCCTAC
    CAGCACTCAG GAGGCGGCGC CCGGGAGGAC TATTTTACTT TTCTAGCCAC AGACAGGAAG
    AACCAAGGCT TTGTTGTGGA TGGGAAAGTT CAGAAAGAGC CTGTTCGCTT CACAATCCAG
    GTGGATCAGC TGGACAAGGC AGCGCCCCGT ATCACACACT TGCACTCCCC TACTCAAGTG
    GGGCTCTTGA AAAATGGCTG CTATGGGATT TACATCACTT CCCGTGTGCT GAAGGCATCA
    GACCCTGACA CAGAGGATGA CCAGATCATC TTTAAGATTT TACGAGGCCC ATTGTACGGA
    CGTCTGGAGA ACACAACAAC AGGTGAATTC ATCCACGAGC GATTTAGCCA GAAGGACTTA
    AGCCATAAGA CCATCCTTTA CATCATAAAC CCTTCTTTGC AAGTGACTTC CGATATCCTG
    GAATTTCAGG CCATGGACCC CACAGGGAAC ACTGCCACTC CCCAGAGCTT GGACTTGAGA
    TGGTCGTATA TTGAATGGGC CCAGACTGCA TATGAGGTTT GTGAGAATGT GGGCTTATTG
    CCCTTGGAAG TTACCAGAAG GGGATATCCC ATGGACTCAG CCTTTGTGGG TGTAGAGGTT
    AACCAAGTGT CAGCTACAGT TGGAAAAGAT TTTACTGTCA CTCCATCCAA ACTGCTTCAA
    TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG
    GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCTG TGAATGCGGT TCTTGGCACG
    CAGACAAAAG CGGCGGTGAA AATTTTGGAC TCAAAAGGAG GACGGTGCCA TCCTTCAAAT
    TCCTTCAACC AAAGCAAGCA CAGCACGTGG GGGAAGGGCC CTTGGCATCC GCTGCCCTCA
    GGCTCATCCT CGCTCACCAC TTCAGGTTCT CCTCTTCTGG AAAGGCCTCC TCCATCTTTC
    ACCAGTGGAG ATGCTCTTCA GGGTTTTGGT CTCACCGACC TCACTCAAAG GAAGACAATG
    ACCCAGGGGA ATGGCAAATC AGTTCTCCCA TCCTCTGTTT GTAGAAATGG GACAGACACC
    ATCTACAATT ACCATGGTAT AGTTTCCTTG AAACTAGAGG GCGACAGATT CTCAGCTCAC
    AAACGGAAGG CCAAAATATC CATTGTTAGT CAGCCACAGA GGACAATCAA GGTGGCAGAG
    CTGCCTCTAG CTGATAAGGT GGAATCCACA ACTGATTTGC ACTTTCTCAG ACAGGGTCTG
    CGGCCCTTGT TTCCAAAGAA TTGTTCTGTG GACTTAAAGG GACTCTTTCA TTTTGAAGAA
    AGCACCCACA GATTGTATCA ATGTGATGGG ATCTCCTGGA AAGCCTGGAG CCCCCAAACC
    AAGGGGCTGG AGGACAGATC CTGCCCAGGT GGATGGCTCC TTCATTCTGG CTACTGTCAC
    ATCTTGGTCA CAAGACAGAA AGGCACCTGG ACCACAGCTA CCCGAGCTTG CAGAGAACAG
    CACCAGGGCG ACCTTGTGAC CGTACTCTCC AGAAGGCATA TGCAGTGGCT ATGGGCGATG
    AGTGGAAGAA AACCCTTCTG GATAGGCTTG AAGAACCAAC CGAGGACTGG CCACTGGGAG
    TGGATCGGAG GTGAACCTGT TGCTTTTACC AACTGGAGGA GAGGGGCCCC TCTGCACCCA
    AAACCTGGAA AGAACTGTGC TTTGGTTCAG AAAAGAGGAC AATGGCAGAC AAAGAACTGT
    AGCAAAGGCA AGGCTCACAA TTTTGTGTGC TCAAGGAAAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006538081.1
    CDS110..4594
    Translation

    Target ORF information:

    RefSeq Version XM_006538018.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006538018.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACCAGCT TCCTAAAGAA GCTCCTGGCA TTTCCCGGCA TTTGGTTGTC AAGGAAACAG 
    AGACACTTGG ATGCTGGGAA GCTATTCATG GTGGACAGCA TACCAAAGCT AACCAAAAAT
    CCGACAGCCC CAGGGCTAAG TTCATTTACT CAGCATGCCG TGAACCATAT GAAAGTGGCC
    TACATGCCAC CCATGCAAGA TATAGGCCCC TCCCCTCGAC ATGTGCAGTT CACAGTGTCT
    GTCAGTAACC AGCATGGAGG TGCTTTGCAT GGAATCTGCT TCAACATCAC AGTTCTCCCA
    GTGGACAACC AGGTTCCCGA GGTATTCACC AACACCCTGA GAGTGGTCGA GGGCGGTCAG
    TGCACCATCA GCACAGAGCA CATTTTGGTT TCTGATGTGG ACACCCCGCT AGACAGCATC
    AGCCTCTCTC TTAAGGAGAG GCCTCTGCAT GGCGGGGTAG AGCTGGACGG ATTTCCTCTA
    AACCCAAGAG GCACATTTTC TTGGCGAGAC CTCAATACCT TAAAAGTTTG GTATCAACAT
    GATGGATCTG AGGTTCTTCA GGATGAGATA TTTTTGGAGG TCACAGATGG CACAAATTCA
    GCAGCATTTG TTCTCCACAT TGAGGTGTTC CCTGTGAATG ATGAGCCACC CATTCTGAAG
    GCTGACCTCA TCCCCATGAT GCACTGCTCT GAGGGTGGAG AGGTGACCAT CACCCCTGAA
    TACATTTCCG CTACTGATGC AGACAGCGAT GACTTAGAAC TGCTGTTTCT GATTGCCCGA
    GAGCCTCAGC ATGGAGTGGT GAGAAAGGCT GGACTTCACG TGGACAGGTT CTCTCAGGGA
    GATGTCATCT CAGGGGCTGT GACATACAAA CATACAGGTG GCGAGATTGG CCTTGAACCT
    TGTTCTGACA CTGTTGTATT GGTGGTTTCG GATGGTGAAG CTGACCCTTT GATGAACGGC
    TGTTGCTACG ATGGACCTGA TTCATCTGTT CCTCTTCATA AGTCCTTCCC CACATACCAG
    CTCAACATCA CAGTACATCC AGTCGACAAC CAGCCACCTT CAATTATAAT AGGTAGGATG
    CTCACAGTGG ATGAAGGCTT CTCAGCAGCC CTTACTACTC ATCACTTGAC TGTTGTTGAT
    TGGGACACTG CCCCGGATGA CTTAAAATTC ATGTTGGCTT CTCAGCCCCA GTTTGGCTAC
    TTAGAAAACG CACTGCCTTC TGCAGGCTTT GAGAAAAGTA ACATCGGCAT AAGGATAGCC
    TCATTCCAGT GGACAGACAT GAAGGCTTCA CACATTAACT ATGTGCAGTC CAGGCACCTG
    AGAGTGGAAC CAACTGCTGA CCAGTTCACA GTATATGCCA CAGACGGGAA GCATCGCTCC
    TTGGAGACAA CATTTCACGT TATCATCAAC CCCACAAACG ATGAAGCTCC TGACCTTGCA
    GTACAGAACA TCACTGTGTA TGAGGGGCAC ATGGTAGAAC TGGATTCTTC CATCATCAGT
    GCTACTGACA GGGATATTCC CAAGGACCCC TTGCTCTTCA GCATCGCTCT CAAGCCCCAG
    CACGGCCTGC TCGTCGATGC TGCCATCAGC AAGGACTCCC ATCAGATCAA GCAGCTGCAA
    CACGAAATCC ACAGCTTCTC CGTGGACCTT CTCAAGAATG GAATGAAGCT GGTCTATGCC
    CACGACGACT CAGAGAGCTC TGCTGATAAC TTCGTGATCC AGTTGTCAGA CGGGAAACAT
    AAAATCCTTA AAACCATCTC AGTAAACATT ACCCCAGTGA ATGACGAGAC ACCAACGCTA
    AGCAAGAAGG CTGAAATCTC AATGGCTGTG GGCGATACTC GCGTCCTGTC TAGTGCTGTT
    CTTTCAGCCA CAGATAAAGA CTCGCCCAGA GAGAAGATTC ACTATGTATT TGAAAGACTT
    CCCCAAAATG GACAACTTCA ACTTAAGATA GGGAGGGACT GGGTCCCTCT CTCCACTGGC
    ATGCAGTGCA CCCAGGAGGA TGTGGACCTG AACTTGCTAA GATACACGCA TGCTGGTAAA
    ACGGACTCCC AGGATGGAGA CAGCTTCACC TTCTACCTCT GGGATGAGGA CAACAGGTCA
    CCTGCATTTG ACTGTCACAT CATCATTGAA GACATGGGCA AAGGTGATAT TGTCATTCAT
    GCAAAACCAC TCGTGGTAGT CAAAGGTGAC AGAGGTCTCT TGACGACTGC CACCCTTCTG
    GCTGTGGATG GAGCAGACAA GCCTGAGGAA CTGCTCTACC TCATCACCTC CCCACCACGG
    CATGGCCAAG TGGAGTACGT CCACTCTCCT GGAGTCCCCA TTGCCAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT GTGCTACATA CACAAGAGCA GGACAGCTGT CCCCACTGAC
    AGCTTCAGAT TTACCATCAG CAATGGACTG CAGACCCAGC GTGGGGTGTT TGAAATCACA
    CTGCAGACTG TGGACAGCGC CTTGCCTGTG CTGACCAAGA ACAAAAGGCT GAGGCTAGCC
    GAAGGGGCCA TGGGCCTCCT GTCCGCTGAT CACCTTCAGC TGACTGACCC CGACACACCT
    CCAGAGAACC TGACCTTCTT TCTGGCTCAG CTCCCACGCC ACGGGTACCT CTTCCTGAGA
    GGGAAAGCAC TCCAACACAA CTTCACCCAG CGAGATGTGG ACAGCGGGGG CGTGGCCTAC
    CAGCACTCAG GAGGCGGCGC CCGGGAGGAC TATTTTACTT TTCTAGCCAC AGACAGGAAG
    AACCAAGGCT TTGTTGTGGA TGGGAAAGTT CAGAAAGAGC CTGTTCGCTT CACAATCCAG
    GTGGATCAGC TGGACAAGGC AGCGCCCCGT ATCACACACT TGCACTCCCC TACTCAAGTG
    GGGCTCTTGA AAAATGGCTG CTATGGGATT TACATCACTT CCCGTGTGCT GAAGGCATCA
    GACCCTGACA CAGAGGATGA CCAGATCATC TTTAAGATTT TACGAGGCCC ATTGTACGGA
    CGTCTGGAGA ACACAACAAC AGGTGAATTC ATCCACGAGC GATTTAGCCA GAAGGACTTA
    AGCCATAAGA CCATCCTTTA CATCATAAAC CCTTCTTTGC AAGTGACTTC CGATATCCTG
    GAATTTCAGG CCATGGACCC CACAGGGAAC ACTGCCACTC CCCAGAGCTT GGACTTGAGA
    TGGTCGTATA TTGAATGGGC CCAGACTGCA TATGAGGTTT GTGAGAATGT GGGCTTATTG
    CCCTTGGAAG TTACCAGAAG GGGATATCCC ATGGACTCAG CCTTTGTGGG TGTAGAGGTT
    AACCAAGTGT CAGCTACAGT TGGAAAAGAT TTTACTGTCA CTCCATCCAA ACTGCTTCAA
    TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG
    GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCTG TGAATGCGGT TCTTGGCACG
    CAGACAAAAG CGGCGGTGAA AATTTTGGAC TCAAAAGGAG GACGGTGCCA TCCTTCAAAT
    TCCTTCAACC AAAGCAAGCA CAGCACGTGG GGGAAGGGCC CTTGGCATCC GCTGCCCTCA
    GGCTCATCCT CGCTCACCAC TTCAGGTTCT CCTCTTCTGG AAAGGCCTCC TCCATCTTTC
    ACCAGTGGAG ATGCTCTTCA GGGTTTTGGT CTCACCGACC TCACTCAAAG GAAGACAATG
    ACCCAGGGGA ATGGCAAATC AGTTCTCCCA TCCTCTGTTT GTAGAAATGG GACAGACACC
    ATCTACAATT ACCATGGTAT AGTTTCCTTG AAACTAGAGG GCGACAGATT CTCAGCTCAC
    AAACGGAAGG CCAAAATATC CATTGTTAGT CAGCCACAGA GGACAATCAA GGTGGCAGAG
    CTGCCTCTAG CTGATAAGGT GGAATCCACA ACTGATTTGC ACTTTCTCAG ACAGGGTCTG
    CGGCCCTTGT TTCCAAAGAA TTGTTCTGTG GACTTAAAGG GACTCTTTCA TTTTGAAGAA
    AGCACCCACA GATTGTATCA ATGTGATGGG ATCTCCTGGA AAGCCTGGAG CCCCCAAACC
    AAGGGGCTGG AGGACAGATC CTGCCCAGGT GGATGGCTCC TTCATTCTGG CTACTGTCAC
    ATCTTGGTCA CAAGACAGAA AGGCACCTGG ACCACAGCTA CCCGAGCTTG CAGAGAACAG
    CACCAGGGCG ACCTTGTGAC CGTACTCTCC AGAAGGCATA TGCAGTGGCT ATGGGCGATG
    AGTGGAAGAA AACCCTTCTG GATAGGCTTG AAGAACCAAC CGAGGACTGG CCACTGGGAG
    TGGATCGGAG GTGAACCTGT TGCTTTTACC AACTGGAGGA GAGGGGCCCC TCTGCACCCA
    AAACCTGGAA AGAACTGTGC TTTGGTTCAG AAAAGAGGAC AATGGCAGAC AAAGAACTGT
    AGCAAAGGCA AGGCTCACAA TTTTGTGTGC TCAAGGAAAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13359
    Clone ID Related Accession (Same CDS sequence) XM_006538013.3 , XM_006538013.4 , XM_006538014.2 , XM_006538014.3 , XM_006538015.2 , NM_001198811.2 , XM_006538015.1 , XM_011250055.3 , NM_001198811.1 , XM_011250055.2
    Accession Version XM_006538013.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538013.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006538076.1
    CDS969..7487
    Translation

    Target ORF information:

    RefSeq Version XM_006538013.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006538013.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13359
    Clone ID Related Accession (Same CDS sequence) XM_006538013.3 , XM_006538013.4 , XM_006538014.2 , XM_006538014.3 , XM_006538015.2 , NM_001198811.2 , XM_006538015.1 , XM_011250055.3 , NM_001198811.1 , XM_011250055.2
    Accession Version XM_006538014.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538014.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006538077.1
    CDS1657..8175
    Translation

    Target ORF information:

    RefSeq Version XM_006538014.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006538014.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13359
    Clone ID Related Accession (Same CDS sequence) XM_006538013.3 , XM_006538013.4 , XM_006538014.2 , XM_006538014.3 , XM_006538015.2 , NM_001198811.2 , XM_006538015.1 , XM_011250055.3 , NM_001198811.1 , XM_011250055.2
    Accession Version XM_006538015.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006538078.1
    CDS612..7130
    Translation

    Target ORF information:

    RefSeq Version XM_006538015.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006538015.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13359
    Clone ID Related Accession (Same CDS sequence) XM_006538013.3 , XM_006538013.4 , XM_006538014.2 , XM_006538014.3 , XM_006538015.2 , NM_001198811.2 , XM_006538015.1 , XM_011250055.3 , NM_001198811.1 , XM_011250055.2
    Accession Version XM_011250055.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250055.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011248357.1
    CDS1081..7599
    Translation

    Target ORF information:

    RefSeq Version XM_011250055.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011250055.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13359
    Clone ID Related Accession (Same CDS sequence) XM_006538013.3 , XM_006538013.4 , XM_006538014.2 , XM_006538014.3 , XM_006538015.2 , NM_001198811.2 , XM_006538015.1 , XM_011250055.3 , NM_001198811.1 , XM_011250055.2
    Accession Version NM_001198811.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform 2 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB160986.1. On Mar 17, 2018 this sequence version replaced NM_001198811.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB160986.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001185740.1
    CDS526..7044
    Translation

    Target ORF information:

    RefSeq Version NM_001198811.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001198811.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13359
    Clone ID Related Accession (Same CDS sequence) XM_006538013.3 , XM_006538013.4 , XM_006538014.2 , XM_006538014.3 , XM_006538015.2 , NM_001198811.2 , XM_006538015.1 , XM_011250055.3 , NM_001198811.1 , XM_011250055.2
    Accession Version NM_001198811.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-31
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform 2 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB160986.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in a 5' coding exon, compared to variant 1, which results in a protein (isoform 2) that is 19 aa shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB160986.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001185740.1
    CDS527..7045
    Translation

    Target ORF information:

    RefSeq Version NM_001198811.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001198811.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13403
    Clone ID Related Accession (Same CDS sequence) NM_177863.4 , NM_177863.5
    Accession Version NM_177863.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-11-01
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform 1 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL670958.4. On Nov 17, 2006 this sequence version replaced NM_177863.3. Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ616838.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGGGC TTGATTCTGA TCTCTGCAAA TCAGCACACG
    TGTTCATCTC AATTTGCTTC ACAGCATGCC GTGAACCATA TGAAAGTGGC CTACATGCCA
    CCCATGCAAG ATATAGGCCC CTCCCCTCGA CATGTGCAGT TCACAGTGTC TGTCAGTAAC
    CAGCATGGAG GTGCTTTGCA TGGAATCTGC TTCAACATCA CAGTTCTCCC AGTGGACAAC
    CAGGTTCCCG AGGTATTCAC CAACACCCTG AGAGTGGTCG AGGGCGGTCA GTGCACCATC
    AGCACAGAGC ACATTTTGGT TTCTGATGTG GACACCCCGC TAGACAGCAT CAGCCTCTCT
    CTTAAGGAGA GGCCTCTGCA TGGCGGGGTA GAGCTGGACG GATTTCCTCT AAACCCAAGA
    GGCACATTTT CTTGGCGAGA CCTCAATACC TTAAAAGTTT GGTATCAACA TGATGGATCT
    GAGGTTCTTC AGGATGAGAT ATTTTTGGAG GTCACAGATG GCACAAATTC AGCAGCATTT
    GTTCTCCACA TTGAGGTGTT CCCTGTGAAT GATGAGCCAC CCATTCTGAA GGCTGACCTC
    ATCCCCATGA TGCACTGCTC TGAGGGTGGA GAGGTGACCA TCACCCCTGA ATACATTTCC
    GCTACTGATG CAGACAGCGA TGACTTAGAA CTGCTGTTTC TGATTGCCCG AGAGCCTCAG
    CATGGAGTGG TGAGAAAGGC TGGACTTCAC GTGGACAGGT TCTCTCAGGG AGATGTCATC
    TCAGGGGCTG TGACATACAA ACATACAGGT GGCGAGATTG GCCTTGAACC TTGTTCTGAC
    ACTGTTGTAT TGGTGGTTTC GGATGGTGAA GCTGACCCTT TGATGAACGG CTGTTGCTAC
    GATGGACCTG ATTCATCTGT TCCTCTTCAT AAGTCCTTCC CCACATACCA GCTCAACATC
    ACAGTACATC CAGTCGACAA CCAGCCACCT TCAATTATAA TAGGTAGGAT GCTCACAGTG
    GATGAAGGCT TCTCAGCAGC CCTTACTACT CATCACTTGA CTGTTGTTGA TTGGGACACT
    GCCCCGGATG ACTTAAAATT CATGTTGGCT TCTCAGCCCC AGTTTGGCTA CTTAGAAAAC
    GCACTGCCTT CTGCAGGCTT TGAGAAAAGT AACATCGGCA TAAGGATAGC CTCATTCCAG
    TGGACAGACA TGAAGGCTTC ACACATTAAC TATGTGCAGT CCAGGCACCT GAGAGTGGAA
    CCAACTGCTG ACCAGTTCAC AGTATATGCC ACAGACGGGA AGCATCGCTC CTTGGAGACA
    ACATTTCACG TTATCATCAA CCCCACAAAC GATGAAGCTC CTGACCTTGC AGTACAGAAC
    ATCACTGTGT ATGAGGGGCA CATGGTAGAA CTGGATTCTT CCATCATCAG TGCTACTGAC
    AGGGATATTC CCAAGGACCC CTTGCTCTTC AGCATCGCTC TCAAGCCCCA GCACGGCCTG
    CTCGTCGATG CTGCCATCAG CAAGGACTCC CATCAGATCA AGCAGCTGCA ACACGAAATC
    CACAGCTTCT CCGTGGACCT TCTCAAGAAT GGAATGAAGC TGGTCTATGC CCACGACGAC
    TCAGAGAGCT CTGCTGATAA CTTCGTGATC CAGTTGTCAG ACGGGAAACA TAAAATCCTT
    AAAACCATCT CAGTAAACAT TACCCCAGTG AATGACGAGA CACCAACGCT AAGCAAGAAG
    GCTGAAATCT CAATGGCTGT GGGCGATACT CGCGTCCTGT CTAGTGCTGT TCTTTCAGCC
    ACAGATAAAG ACTCGCCCAG AGAGAAGATT CACTATGTAT TTGAAAGACT TCCCCAAAAT
    GGACAACTTC AACTTAAGAT AGGGAGGGAC TGGGTCCCTC TCTCCACTGG CATGCAGTGC
    ACCCAGGAGG ATGTGGACCT GAACTTGCTA AGATACACGC ATGCTGGTAA AACGGACTCC
    CAGGATGGAG ACAGCTTCAC CTTCTACCTC TGGGATGAGG ACAACAGGTC ACCTGCATTT
    GACTGTCACA TCATCATTGA AGACATGGGC AAAGGTGATA TTGTCATTCA TGCAAAACCA
    CTCGTGGTAG TCAAAGGTGA CAGAGGTCTC TTGACGACTG CCACCCTTCT GGCTGTGGAT
    GGAGCAGACA AGCCTGAGGA ACTGCTCTAC CTCATCACCT CCCCACCACG GCATGGCCAA
    GTGGAGTACG TCCACTCTCC TGGAGTCCCC ATTGCCAGCT TCAGCCAAAT GGACATAGCA
    GGACAGACAG TGTGCTACAT ACACAAGAGC AGGACAGCTG TCCCCACTGA CAGCTTCAGA
    TTTACCATCA GCAATGGACT GCAGACCCAG CGTGGGGTGT TTGAAATCAC ACTGCAGACT
    GTGGACAGCG CCTTGCCTGT GCTGACCAAG AACAAAAGGC TGAGGCTAGC CGAAGGGGCC
    ATGGGCCTCC TGTCCGCTGA TCACCTTCAG CTGACTGACC CCGACACACC TCCAGAGAAC
    CTGACCTTCT TTCTGGCTCA GCTCCCACGC CACGGGTACC TCTTCCTGAG AGGGAAAGCA
    CTCCAACACA ACTTCACCCA GCGAGATGTG GACAGCGGGG GCGTGGCCTA CCAGCACTCA
    GGAGGCGGCG CCCGGGAGGA CTATTTTACT TTTCTAGCCA CAGACAGGAA GAACCAAGGC
    TTTGTTGTGG ATGGGAAAGT TCAGAAAGAG CCTGTTCGCT TCACAATCCA GGTGGATCAG
    CTGGACAAGG CAGCGCCCCG TATCACACAC TTGCACTCCC CTACTCAAGT GGGGCTCTTG
    AAAAATGGCT GCTATGGGAT TTACATCACT TCCCGTGTGC TGAAGGCATC AGACCCTGAC
    ACAGAGGATG ACCAGATCAT CTTTAAGATT TTACGAGGCC CATTGTACGG ACGTCTGGAG
    AACACAACAA CAGGTGAATT CATCCACGAG CGATTTAGCC AGAAGGACTT AAGCCATAAG
    ACCATCCTTT ACATCATAAA CCCTTCTTTG CAAGTGACTT CCGATATCCT GGAATTTCAG
    GCCATGGACC CCACAGGGAA CACTGCCACT CCCCAGAGCT TGGACTTGAG ATGGTCGTAT
    ATTGAATGGG CCCAGACTGC ATATGAGGTT TGTGAGAATG TGGGCTTATT GCCCTTGGAA
    GTTACCAGAA GGGGATATCC CATGGACTCA GCCTTTGTGG GTGTAGAGGT TAACCAAGTG
    TCAGCTACAG TTGGAAAAGA TTTTACTGTC ACTCCATCCA AACTGCTTCA ATTTGACCCA
    GGAATGTCAA CTAAGATGTG GAATATAGCA ATTACCTATG ACGGATTAGA GGAAGATGAT
    GAGGTCTTTG AAGTAATTCT GAACTCCCCT GTGAATGCGG TTCTTGGCAC GCAGACAAAA
    GCGGCGGTGA AAATTTTGGA CTCAAAAGGA GGACGGTGCC ATCCTTCAAA TTCCTTCAAC
    CAAAGCAAGC ACAGCACGTG GGGGAAGGGC CCTTGGCATC CGCTGCCCTC AGGCTCATCC
    TCGCTCACCA CTTCAGGTTC TCCTCTTCTG GAAAGGCCTC CTCCATCTTT CACCAGTGGA
    GATGCTCTTC AGGGTTTTGG TCTCACCGAC CTCACTCAAA GGAAGACAAT GACCCAGGGG
    AATGGCAAAT CAGTTCTCCC ATCCTCTGTT TGTAGAAATG GGACAGACAC CATCTACAAT
    TACCATGGTA TAGTTTCCTT GAAACTAGAG GGCGACAGAT TCTCAGCTCA CAAACGGAAG
    GCCAAAATAT CCATTGTTAG TCAGCCACAG AGGACAATCA AGGTGGCAGA GCTGCCTCTA
    GCTGATAAGG TGGAATCCAC AACTGATTTG CACTTTCTCA GACAGGGTCT GCGGCCCTTG
    TTTCCAAAGA ATTGTTCTGT GGACTTAAAG GGACTCTTTC ATTTTGAAGA AAGCACCCAC
    AGATTGTATC AATGTGATGG GATCTCCTGG AAAGCCTGGA GCCCCCAAAC CAAGGGGCTG
    GAGGACAGAT CCTGCCCAGG TGGATGGCTC CTTCATTCTG GCTACTGTCA CATCTTGGTC
    ACAAGACAGA AAGGCACCTG GACCACAGCT ACCCGAGCTT GCAGAGAACA GCACCAGGGC
    GACCTTGTGA CCGTACTCTC CAGAAGGCAT ATGCAGTGGC TATGGGCGAT GAGTGGAAGA
    AAACCCTTCT GGATAGGCTT GAAGAACCAA CCGAGGACTG GCCACTGGGA GTGGATCGGA
    GGTGAACCTG TTGCTTTTAC CAACTGGAGG AGAGGGGCCC CTCTGCACCC AAAACCTGGA
    AAGAACTGTG CTTTGGTTCA GAAAAGAGGA CAATGGCAGA CAAAGAACTG TAGCAAAGGC
    AAGGCTCACA ATTTTGTGTG CTCAAGGAAA CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_808531.2
    CDS260..6835
    Translation

    Target ORF information:

    RefSeq Version NM_177863.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_177863.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGGGC TTGATTCTGA TCTCTGCAAA TCAGCACACG
    TGTTCATCTC AATTTGCTTC ACAGCATGCC GTGAACCATA TGAAAGTGGC CTACATGCCA
    CCCATGCAAG ATATAGGCCC CTCCCCTCGA CATGTGCAGT TCACAGTGTC TGTCAGTAAC
    CAGCATGGAG GTGCTTTGCA TGGAATCTGC TTCAACATCA CAGTTCTCCC AGTGGACAAC
    CAGGTTCCCG AGGTATTCAC CAACACCCTG AGAGTGGTCG AGGGCGGTCA GTGCACCATC
    AGCACAGAGC ACATTTTGGT TTCTGATGTG GACACCCCGC TAGACAGCAT CAGCCTCTCT
    CTTAAGGAGA GGCCTCTGCA TGGCGGGGTA GAGCTGGACG GATTTCCTCT AAACCCAAGA
    GGCACATTTT CTTGGCGAGA CCTCAATACC TTAAAAGTTT GGTATCAACA TGATGGATCT
    GAGGTTCTTC AGGATGAGAT ATTTTTGGAG GTCACAGATG GCACAAATTC AGCAGCATTT
    GTTCTCCACA TTGAGGTGTT CCCTGTGAAT GATGAGCCAC CCATTCTGAA GGCTGACCTC
    ATCCCCATGA TGCACTGCTC TGAGGGTGGA GAGGTGACCA TCACCCCTGA ATACATTTCC
    GCTACTGATG CAGACAGCGA TGACTTAGAA CTGCTGTTTC TGATTGCCCG AGAGCCTCAG
    CATGGAGTGG TGAGAAAGGC TGGACTTCAC GTGGACAGGT TCTCTCAGGG AGATGTCATC
    TCAGGGGCTG TGACATACAA ACATACAGGT GGCGAGATTG GCCTTGAACC TTGTTCTGAC
    ACTGTTGTAT TGGTGGTTTC GGATGGTGAA GCTGACCCTT TGATGAACGG CTGTTGCTAC
    GATGGACCTG ATTCATCTGT TCCTCTTCAT AAGTCCTTCC CCACATACCA GCTCAACATC
    ACAGTACATC CAGTCGACAA CCAGCCACCT TCAATTATAA TAGGTAGGAT GCTCACAGTG
    GATGAAGGCT TCTCAGCAGC CCTTACTACT CATCACTTGA CTGTTGTTGA TTGGGACACT
    GCCCCGGATG ACTTAAAATT CATGTTGGCT TCTCAGCCCC AGTTTGGCTA CTTAGAAAAC
    GCACTGCCTT CTGCAGGCTT TGAGAAAAGT AACATCGGCA TAAGGATAGC CTCATTCCAG
    TGGACAGACA TGAAGGCTTC ACACATTAAC TATGTGCAGT CCAGGCACCT GAGAGTGGAA
    CCAACTGCTG ACCAGTTCAC AGTATATGCC ACAGACGGGA AGCATCGCTC CTTGGAGACA
    ACATTTCACG TTATCATCAA CCCCACAAAC GATGAAGCTC CTGACCTTGC AGTACAGAAC
    ATCACTGTGT ATGAGGGGCA CATGGTAGAA CTGGATTCTT CCATCATCAG TGCTACTGAC
    AGGGATATTC CCAAGGACCC CTTGCTCTTC AGCATCGCTC TCAAGCCCCA GCACGGCCTG
    CTCGTCGATG CTGCCATCAG CAAGGACTCC CATCAGATCA AGCAGCTGCA ACACGAAATC
    CACAGCTTCT CCGTGGACCT TCTCAAGAAT GGAATGAAGC TGGTCTATGC CCACGACGAC
    TCAGAGAGCT CTGCTGATAA CTTCGTGATC CAGTTGTCAG ACGGGAAACA TAAAATCCTT
    AAAACCATCT CAGTAAACAT TACCCCAGTG AATGACGAGA CACCAACGCT AAGCAAGAAG
    GCTGAAATCT CAATGGCTGT GGGCGATACT CGCGTCCTGT CTAGTGCTGT TCTTTCAGCC
    ACAGATAAAG ACTCGCCCAG AGAGAAGATT CACTATGTAT TTGAAAGACT TCCCCAAAAT
    GGACAACTTC AACTTAAGAT AGGGAGGGAC TGGGTCCCTC TCTCCACTGG CATGCAGTGC
    ACCCAGGAGG ATGTGGACCT GAACTTGCTA AGATACACGC ATGCTGGTAA AACGGACTCC
    CAGGATGGAG ACAGCTTCAC CTTCTACCTC TGGGATGAGG ACAACAGGTC ACCTGCATTT
    GACTGTCACA TCATCATTGA AGACATGGGC AAAGGTGATA TTGTCATTCA TGCAAAACCA
    CTCGTGGTAG TCAAAGGTGA CAGAGGTCTC TTGACGACTG CCACCCTTCT GGCTGTGGAT
    GGAGCAGACA AGCCTGAGGA ACTGCTCTAC CTCATCACCT CCCCACCACG GCATGGCCAA
    GTGGAGTACG TCCACTCTCC TGGAGTCCCC ATTGCCAGCT TCAGCCAAAT GGACATAGCA
    GGACAGACAG TGTGCTACAT ACACAAGAGC AGGACAGCTG TCCCCACTGA CAGCTTCAGA
    TTTACCATCA GCAATGGACT GCAGACCCAG CGTGGGGTGT TTGAAATCAC ACTGCAGACT
    GTGGACAGCG CCTTGCCTGT GCTGACCAAG AACAAAAGGC TGAGGCTAGC CGAAGGGGCC
    ATGGGCCTCC TGTCCGCTGA TCACCTTCAG CTGACTGACC CCGACACACC TCCAGAGAAC
    CTGACCTTCT TTCTGGCTCA GCTCCCACGC CACGGGTACC TCTTCCTGAG AGGGAAAGCA
    CTCCAACACA ACTTCACCCA GCGAGATGTG GACAGCGGGG GCGTGGCCTA CCAGCACTCA
    GGAGGCGGCG CCCGGGAGGA CTATTTTACT TTTCTAGCCA CAGACAGGAA GAACCAAGGC
    TTTGTTGTGG ATGGGAAAGT TCAGAAAGAG CCTGTTCGCT TCACAATCCA GGTGGATCAG
    CTGGACAAGG CAGCGCCCCG TATCACACAC TTGCACTCCC CTACTCAAGT GGGGCTCTTG
    AAAAATGGCT GCTATGGGAT TTACATCACT TCCCGTGTGC TGAAGGCATC AGACCCTGAC
    ACAGAGGATG ACCAGATCAT CTTTAAGATT TTACGAGGCC CATTGTACGG ACGTCTGGAG
    AACACAACAA CAGGTGAATT CATCCACGAG CGATTTAGCC AGAAGGACTT AAGCCATAAG
    ACCATCCTTT ACATCATAAA CCCTTCTTTG CAAGTGACTT CCGATATCCT GGAATTTCAG
    GCCATGGACC CCACAGGGAA CACTGCCACT CCCCAGAGCT TGGACTTGAG ATGGTCGTAT
    ATTGAATGGG CCCAGACTGC ATATGAGGTT TGTGAGAATG TGGGCTTATT GCCCTTGGAA
    GTTACCAGAA GGGGATATCC CATGGACTCA GCCTTTGTGG GTGTAGAGGT TAACCAAGTG
    TCAGCTACAG TTGGAAAAGA TTTTACTGTC ACTCCATCCA AACTGCTTCA ATTTGACCCA
    GGAATGTCAA CTAAGATGTG GAATATAGCA ATTACCTATG ACGGATTAGA GGAAGATGAT
    GAGGTCTTTG AAGTAATTCT GAACTCCCCT GTGAATGCGG TTCTTGGCAC GCAGACAAAA
    GCGGCGGTGA AAATTTTGGA CTCAAAAGGA GGACGGTGCC ATCCTTCAAA TTCCTTCAAC
    CAAAGCAAGC ACAGCACGTG GGGGAAGGGC CCTTGGCATC CGCTGCCCTC AGGCTCATCC
    TCGCTCACCA CTTCAGGTTC TCCTCTTCTG GAAAGGCCTC CTCCATCTTT CACCAGTGGA
    GATGCTCTTC AGGGTTTTGG TCTCACCGAC CTCACTCAAA GGAAGACAAT GACCCAGGGG
    AATGGCAAAT CAGTTCTCCC ATCCTCTGTT TGTAGAAATG GGACAGACAC CATCTACAAT
    TACCATGGTA TAGTTTCCTT GAAACTAGAG GGCGACAGAT TCTCAGCTCA CAAACGGAAG
    GCCAAAATAT CCATTGTTAG TCAGCCACAG AGGACAATCA AGGTGGCAGA GCTGCCTCTA
    GCTGATAAGG TGGAATCCAC AACTGATTTG CACTTTCTCA GACAGGGTCT GCGGCCCTTG
    TTTCCAAAGA ATTGTTCTGT GGACTTAAAG GGACTCTTTC ATTTTGAAGA AAGCACCCAC
    AGATTGTATC AATGTGATGG GATCTCCTGG AAAGCCTGGA GCCCCCAAAC CAAGGGGCTG
    GAGGACAGAT CCTGCCCAGG TGGATGGCTC CTTCATTCTG GCTACTGTCA CATCTTGGTC
    ACAAGACAGA AAGGCACCTG GACCACAGCT ACCCGAGCTT GCAGAGAACA GCACCAGGGC
    GACCTTGTGA CCGTACTCTC CAGAAGGCAT ATGCAGTGGC TATGGGCGAT GAGTGGAAGA
    AAACCCTTCT GGATAGGCTT GAAGAACCAA CCGAGGACTG GCCACTGGGA GTGGATCGGA
    GGTGAACCTG TTGCTTTTAC CAACTGGAGG AGAGGGGCCC CTCTGCACCC AAAACCTGGA
    AAGAACTGTG CTTTGGTTCA GAAAAGAGGA CAATGGCAGA CAAAGAACTG TAGCAAAGGC
    AAGGCTCACA ATTTTGTGTG CTCAAGGAAA CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13403
    Clone ID Related Accession (Same CDS sequence) NM_177863.4 , NM_177863.5
    Accession Version NM_177863.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-06
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL670958.4. On Mar 19, 2018 this sequence version replaced NM_177863.4. Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ616838.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGGGC TTGATTCTGA TCTCTGCAAA TCAGCACACG
    TGTTCATCTC AATTTGCTTC ACAGCATGCC GTGAACCATA TGAAAGTGGC CTACATGCCA
    CCCATGCAAG ATATAGGCCC CTCCCCTCGA CATGTGCAGT TCACAGTGTC TGTCAGTAAC
    CAGCATGGAG GTGCTTTGCA TGGAATCTGC TTCAACATCA CAGTTCTCCC AGTGGACAAC
    CAGGTTCCCG AGGTATTCAC CAACACCCTG AGAGTGGTCG AGGGCGGTCA GTGCACCATC
    AGCACAGAGC ACATTTTGGT TTCTGATGTG GACACCCCGC TAGACAGCAT CAGCCTCTCT
    CTTAAGGAGA GGCCTCTGCA TGGCGGGGTA GAGCTGGACG GATTTCCTCT AAACCCAAGA
    GGCACATTTT CTTGGCGAGA CCTCAATACC TTAAAAGTTT GGTATCAACA TGATGGATCT
    GAGGTTCTTC AGGATGAGAT ATTTTTGGAG GTCACAGATG GCACAAATTC AGCAGCATTT
    GTTCTCCACA TTGAGGTGTT CCCTGTGAAT GATGAGCCAC CCATTCTGAA GGCTGACCTC
    ATCCCCATGA TGCACTGCTC TGAGGGTGGA GAGGTGACCA TCACCCCTGA ATACATTTCC
    GCTACTGATG CAGACAGCGA TGACTTAGAA CTGCTGTTTC TGATTGCCCG AGAGCCTCAG
    CATGGAGTGG TGAGAAAGGC TGGACTTCAC GTGGACAGGT TCTCTCAGGG AGATGTCATC
    TCAGGGGCTG TGACATACAA ACATACAGGT GGCGAGATTG GCCTTGAACC TTGTTCTGAC
    ACTGTTGTAT TGGTGGTTTC GGATGGTGAA GCTGACCCTT TGATGAACGG CTGTTGCTAC
    GATGGACCTG ATTCATCTGT TCCTCTTCAT AAGTCCTTCC CCACATACCA GCTCAACATC
    ACAGTACATC CAGTCGACAA CCAGCCACCT TCAATTATAA TAGGTAGGAT GCTCACAGTG
    GATGAAGGCT TCTCAGCAGC CCTTACTACT CATCACTTGA CTGTTGTTGA TTGGGACACT
    GCCCCGGATG ACTTAAAATT CATGTTGGCT TCTCAGCCCC AGTTTGGCTA CTTAGAAAAC
    GCACTGCCTT CTGCAGGCTT TGAGAAAAGT AACATCGGCA TAAGGATAGC CTCATTCCAG
    TGGACAGACA TGAAGGCTTC ACACATTAAC TATGTGCAGT CCAGGCACCT GAGAGTGGAA
    CCAACTGCTG ACCAGTTCAC AGTATATGCC ACAGACGGGA AGCATCGCTC CTTGGAGACA
    ACATTTCACG TTATCATCAA CCCCACAAAC GATGAAGCTC CTGACCTTGC AGTACAGAAC
    ATCACTGTGT ATGAGGGGCA CATGGTAGAA CTGGATTCTT CCATCATCAG TGCTACTGAC
    AGGGATATTC CCAAGGACCC CTTGCTCTTC AGCATCGCTC TCAAGCCCCA GCACGGCCTG
    CTCGTCGATG CTGCCATCAG CAAGGACTCC CATCAGATCA AGCAGCTGCA ACACGAAATC
    CACAGCTTCT CCGTGGACCT TCTCAAGAAT GGAATGAAGC TGGTCTATGC CCACGACGAC
    TCAGAGAGCT CTGCTGATAA CTTCGTGATC CAGTTGTCAG ACGGGAAACA TAAAATCCTT
    AAAACCATCT CAGTAAACAT TACCCCAGTG AATGACGAGA CACCAACGCT AAGCAAGAAG
    GCTGAAATCT CAATGGCTGT GGGCGATACT CGCGTCCTGT CTAGTGCTGT TCTTTCAGCC
    ACAGATAAAG ACTCGCCCAG AGAGAAGATT CACTATGTAT TTGAAAGACT TCCCCAAAAT
    GGACAACTTC AACTTAAGAT AGGGAGGGAC TGGGTCCCTC TCTCCACTGG CATGCAGTGC
    ACCCAGGAGG ATGTGGACCT GAACTTGCTA AGATACACGC ATGCTGGTAA AACGGACTCC
    CAGGATGGAG ACAGCTTCAC CTTCTACCTC TGGGATGAGG ACAACAGGTC ACCTGCATTT
    GACTGTCACA TCATCATTGA AGACATGGGC AAAGGTGATA TTGTCATTCA TGCAAAACCA
    CTCGTGGTAG TCAAAGGTGA CAGAGGTCTC TTGACGACTG CCACCCTTCT GGCTGTGGAT
    GGAGCAGACA AGCCTGAGGA ACTGCTCTAC CTCATCACCT CCCCACCACG GCATGGCCAA
    GTGGAGTACG TCCACTCTCC TGGAGTCCCC ATTGCCAGCT TCAGCCAAAT GGACATAGCA
    GGACAGACAG TGTGCTACAT ACACAAGAGC AGGACAGCTG TCCCCACTGA CAGCTTCAGA
    TTTACCATCA GCAATGGACT GCAGACCCAG CGTGGGGTGT TTGAAATCAC ACTGCAGACT
    GTGGACAGCG CCTTGCCTGT GCTGACCAAG AACAAAAGGC TGAGGCTAGC CGAAGGGGCC
    ATGGGCCTCC TGTCCGCTGA TCACCTTCAG CTGACTGACC CCGACACACC TCCAGAGAAC
    CTGACCTTCT TTCTGGCTCA GCTCCCACGC CACGGGTACC TCTTCCTGAG AGGGAAAGCA
    CTCCAACACA ACTTCACCCA GCGAGATGTG GACAGCGGGG GCGTGGCCTA CCAGCACTCA
    GGAGGCGGCG CCCGGGAGGA CTATTTTACT TTTCTAGCCA CAGACAGGAA GAACCAAGGC
    TTTGTTGTGG ATGGGAAAGT TCAGAAAGAG CCTGTTCGCT TCACAATCCA GGTGGATCAG
    CTGGACAAGG CAGCGCCCCG TATCACACAC TTGCACTCCC CTACTCAAGT GGGGCTCTTG
    AAAAATGGCT GCTATGGGAT TTACATCACT TCCCGTGTGC TGAAGGCATC AGACCCTGAC
    ACAGAGGATG ACCAGATCAT CTTTAAGATT TTACGAGGCC CATTGTACGG ACGTCTGGAG
    AACACAACAA CAGGTGAATT CATCCACGAG CGATTTAGCC AGAAGGACTT AAGCCATAAG
    ACCATCCTTT ACATCATAAA CCCTTCTTTG CAAGTGACTT CCGATATCCT GGAATTTCAG
    GCCATGGACC CCACAGGGAA CACTGCCACT CCCCAGAGCT TGGACTTGAG ATGGTCGTAT
    ATTGAATGGG CCCAGACTGC ATATGAGGTT TGTGAGAATG TGGGCTTATT GCCCTTGGAA
    GTTACCAGAA GGGGATATCC CATGGACTCA GCCTTTGTGG GTGTAGAGGT TAACCAAGTG
    TCAGCTACAG TTGGAAAAGA TTTTACTGTC ACTCCATCCA AACTGCTTCA ATTTGACCCA
    GGAATGTCAA CTAAGATGTG GAATATAGCA ATTACCTATG ACGGATTAGA GGAAGATGAT
    GAGGTCTTTG AAGTAATTCT GAACTCCCCT GTGAATGCGG TTCTTGGCAC GCAGACAAAA
    GCGGCGGTGA AAATTTTGGA CTCAAAAGGA GGACGGTGCC ATCCTTCAAA TTCCTTCAAC
    CAAAGCAAGC ACAGCACGTG GGGGAAGGGC CCTTGGCATC CGCTGCCCTC AGGCTCATCC
    TCGCTCACCA CTTCAGGTTC TCCTCTTCTG GAAAGGCCTC CTCCATCTTT CACCAGTGGA
    GATGCTCTTC AGGGTTTTGG TCTCACCGAC CTCACTCAAA GGAAGACAAT GACCCAGGGG
    AATGGCAAAT CAGTTCTCCC ATCCTCTGTT TGTAGAAATG GGACAGACAC CATCTACAAT
    TACCATGGTA TAGTTTCCTT GAAACTAGAG GGCGACAGAT TCTCAGCTCA CAAACGGAAG
    GCCAAAATAT CCATTGTTAG TCAGCCACAG AGGACAATCA AGGTGGCAGA GCTGCCTCTA
    GCTGATAAGG TGGAATCCAC AACTGATTTG CACTTTCTCA GACAGGGTCT GCGGCCCTTG
    TTTCCAAAGA ATTGTTCTGT GGACTTAAAG GGACTCTTTC ATTTTGAAGA AAGCACCCAC
    AGATTGTATC AATGTGATGG GATCTCCTGG AAAGCCTGGA GCCCCCAAAC CAAGGGGCTG
    GAGGACAGAT CCTGCCCAGG TGGATGGCTC CTTCATTCTG GCTACTGTCA CATCTTGGTC
    ACAAGACAGA AAGGCACCTG GACCACAGCT ACCCGAGCTT GCAGAGAACA GCACCAGGGC
    GACCTTGTGA CCGTACTCTC CAGAAGGCAT ATGCAGTGGC TATGGGCGAT GAGTGGAAGA
    AAACCCTTCT GGATAGGCTT GAAGAACCAA CCGAGGACTG GCCACTGGGA GTGGATCGGA
    GGTGAACCTG TTGCTTTTAC CAACTGGAGG AGAGGGGCCC CTCTGCACCC AAAACCTGGA
    AAGAACTGTG CTTTGGTTCA GAAAAGAGGA CAATGGCAGA CAAAGAACTG TAGCAAAGGC
    AAGGCTCACA ATTTTGTGTG CTCAAGGAAA CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_808531.2
    CDS260..6835
    Translation

    Target ORF information:

    RefSeq Version NM_177863.5
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_177863.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGGGC TTGATTCTGA TCTCTGCAAA TCAGCACACG
    TGTTCATCTC AATTTGCTTC ACAGCATGCC GTGAACCATA TGAAAGTGGC CTACATGCCA
    CCCATGCAAG ATATAGGCCC CTCCCCTCGA CATGTGCAGT TCACAGTGTC TGTCAGTAAC
    CAGCATGGAG GTGCTTTGCA TGGAATCTGC TTCAACATCA CAGTTCTCCC AGTGGACAAC
    CAGGTTCCCG AGGTATTCAC CAACACCCTG AGAGTGGTCG AGGGCGGTCA GTGCACCATC
    AGCACAGAGC ACATTTTGGT TTCTGATGTG GACACCCCGC TAGACAGCAT CAGCCTCTCT
    CTTAAGGAGA GGCCTCTGCA TGGCGGGGTA GAGCTGGACG GATTTCCTCT AAACCCAAGA
    GGCACATTTT CTTGGCGAGA CCTCAATACC TTAAAAGTTT GGTATCAACA TGATGGATCT
    GAGGTTCTTC AGGATGAGAT ATTTTTGGAG GTCACAGATG GCACAAATTC AGCAGCATTT
    GTTCTCCACA TTGAGGTGTT CCCTGTGAAT GATGAGCCAC CCATTCTGAA GGCTGACCTC
    ATCCCCATGA TGCACTGCTC TGAGGGTGGA GAGGTGACCA TCACCCCTGA ATACATTTCC
    GCTACTGATG CAGACAGCGA TGACTTAGAA CTGCTGTTTC TGATTGCCCG AGAGCCTCAG
    CATGGAGTGG TGAGAAAGGC TGGACTTCAC GTGGACAGGT TCTCTCAGGG AGATGTCATC
    TCAGGGGCTG TGACATACAA ACATACAGGT GGCGAGATTG GCCTTGAACC TTGTTCTGAC
    ACTGTTGTAT TGGTGGTTTC GGATGGTGAA GCTGACCCTT TGATGAACGG CTGTTGCTAC
    GATGGACCTG ATTCATCTGT TCCTCTTCAT AAGTCCTTCC CCACATACCA GCTCAACATC
    ACAGTACATC CAGTCGACAA CCAGCCACCT TCAATTATAA TAGGTAGGAT GCTCACAGTG
    GATGAAGGCT TCTCAGCAGC CCTTACTACT CATCACTTGA CTGTTGTTGA TTGGGACACT
    GCCCCGGATG ACTTAAAATT CATGTTGGCT TCTCAGCCCC AGTTTGGCTA CTTAGAAAAC
    GCACTGCCTT CTGCAGGCTT TGAGAAAAGT AACATCGGCA TAAGGATAGC CTCATTCCAG
    TGGACAGACA TGAAGGCTTC ACACATTAAC TATGTGCAGT CCAGGCACCT GAGAGTGGAA
    CCAACTGCTG ACCAGTTCAC AGTATATGCC ACAGACGGGA AGCATCGCTC CTTGGAGACA
    ACATTTCACG TTATCATCAA CCCCACAAAC GATGAAGCTC CTGACCTTGC AGTACAGAAC
    ATCACTGTGT ATGAGGGGCA CATGGTAGAA CTGGATTCTT CCATCATCAG TGCTACTGAC
    AGGGATATTC CCAAGGACCC CTTGCTCTTC AGCATCGCTC TCAAGCCCCA GCACGGCCTG
    CTCGTCGATG CTGCCATCAG CAAGGACTCC CATCAGATCA AGCAGCTGCA ACACGAAATC
    CACAGCTTCT CCGTGGACCT TCTCAAGAAT GGAATGAAGC TGGTCTATGC CCACGACGAC
    TCAGAGAGCT CTGCTGATAA CTTCGTGATC CAGTTGTCAG ACGGGAAACA TAAAATCCTT
    AAAACCATCT CAGTAAACAT TACCCCAGTG AATGACGAGA CACCAACGCT AAGCAAGAAG
    GCTGAAATCT CAATGGCTGT GGGCGATACT CGCGTCCTGT CTAGTGCTGT TCTTTCAGCC
    ACAGATAAAG ACTCGCCCAG AGAGAAGATT CACTATGTAT TTGAAAGACT TCCCCAAAAT
    GGACAACTTC AACTTAAGAT AGGGAGGGAC TGGGTCCCTC TCTCCACTGG CATGCAGTGC
    ACCCAGGAGG ATGTGGACCT GAACTTGCTA AGATACACGC ATGCTGGTAA AACGGACTCC
    CAGGATGGAG ACAGCTTCAC CTTCTACCTC TGGGATGAGG ACAACAGGTC ACCTGCATTT
    GACTGTCACA TCATCATTGA AGACATGGGC AAAGGTGATA TTGTCATTCA TGCAAAACCA
    CTCGTGGTAG TCAAAGGTGA CAGAGGTCTC TTGACGACTG CCACCCTTCT GGCTGTGGAT
    GGAGCAGACA AGCCTGAGGA ACTGCTCTAC CTCATCACCT CCCCACCACG GCATGGCCAA
    GTGGAGTACG TCCACTCTCC TGGAGTCCCC ATTGCCAGCT TCAGCCAAAT GGACATAGCA
    GGACAGACAG TGTGCTACAT ACACAAGAGC AGGACAGCTG TCCCCACTGA CAGCTTCAGA
    TTTACCATCA GCAATGGACT GCAGACCCAG CGTGGGGTGT TTGAAATCAC ACTGCAGACT
    GTGGACAGCG CCTTGCCTGT GCTGACCAAG AACAAAAGGC TGAGGCTAGC CGAAGGGGCC
    ATGGGCCTCC TGTCCGCTGA TCACCTTCAG CTGACTGACC CCGACACACC TCCAGAGAAC
    CTGACCTTCT TTCTGGCTCA GCTCCCACGC CACGGGTACC TCTTCCTGAG AGGGAAAGCA
    CTCCAACACA ACTTCACCCA GCGAGATGTG GACAGCGGGG GCGTGGCCTA CCAGCACTCA
    GGAGGCGGCG CCCGGGAGGA CTATTTTACT TTTCTAGCCA CAGACAGGAA GAACCAAGGC
    TTTGTTGTGG ATGGGAAAGT TCAGAAAGAG CCTGTTCGCT TCACAATCCA GGTGGATCAG
    CTGGACAAGG CAGCGCCCCG TATCACACAC TTGCACTCCC CTACTCAAGT GGGGCTCTTG
    AAAAATGGCT GCTATGGGAT TTACATCACT TCCCGTGTGC TGAAGGCATC AGACCCTGAC
    ACAGAGGATG ACCAGATCAT CTTTAAGATT TTACGAGGCC CATTGTACGG ACGTCTGGAG
    AACACAACAA CAGGTGAATT CATCCACGAG CGATTTAGCC AGAAGGACTT AAGCCATAAG
    ACCATCCTTT ACATCATAAA CCCTTCTTTG CAAGTGACTT CCGATATCCT GGAATTTCAG
    GCCATGGACC CCACAGGGAA CACTGCCACT CCCCAGAGCT TGGACTTGAG ATGGTCGTAT
    ATTGAATGGG CCCAGACTGC ATATGAGGTT TGTGAGAATG TGGGCTTATT GCCCTTGGAA
    GTTACCAGAA GGGGATATCC CATGGACTCA GCCTTTGTGG GTGTAGAGGT TAACCAAGTG
    TCAGCTACAG TTGGAAAAGA TTTTACTGTC ACTCCATCCA AACTGCTTCA ATTTGACCCA
    GGAATGTCAA CTAAGATGTG GAATATAGCA ATTACCTATG ACGGATTAGA GGAAGATGAT
    GAGGTCTTTG AAGTAATTCT GAACTCCCCT GTGAATGCGG TTCTTGGCAC GCAGACAAAA
    GCGGCGGTGA AAATTTTGGA CTCAAAAGGA GGACGGTGCC ATCCTTCAAA TTCCTTCAAC
    CAAAGCAAGC ACAGCACGTG GGGGAAGGGC CCTTGGCATC CGCTGCCCTC AGGCTCATCC
    TCGCTCACCA CTTCAGGTTC TCCTCTTCTG GAAAGGCCTC CTCCATCTTT CACCAGTGGA
    GATGCTCTTC AGGGTTTTGG TCTCACCGAC CTCACTCAAA GGAAGACAAT GACCCAGGGG
    AATGGCAAAT CAGTTCTCCC ATCCTCTGTT TGTAGAAATG GGACAGACAC CATCTACAAT
    TACCATGGTA TAGTTTCCTT GAAACTAGAG GGCGACAGAT TCTCAGCTCA CAAACGGAAG
    GCCAAAATAT CCATTGTTAG TCAGCCACAG AGGACAATCA AGGTGGCAGA GCTGCCTCTA
    GCTGATAAGG TGGAATCCAC AACTGATTTG CACTTTCTCA GACAGGGTCT GCGGCCCTTG
    TTTCCAAAGA ATTGTTCTGT GGACTTAAAG GGACTCTTTC ATTTTGAAGA AAGCACCCAC
    AGATTGTATC AATGTGATGG GATCTCCTGG AAAGCCTGGA GCCCCCAAAC CAAGGGGCTG
    GAGGACAGAT CCTGCCCAGG TGGATGGCTC CTTCATTCTG GCTACTGTCA CATCTTGGTC
    ACAAGACAGA AAGGCACCTG GACCACAGCT ACCCGAGCTT GCAGAGAACA GCACCAGGGC
    GACCTTGTGA CCGTACTCTC CAGAAGGCAT ATGCAGTGGC TATGGGCGAT GAGTGGAAGA
    AAACCCTTCT GGATAGGCTT GAAGAACCAA CCGAGGACTG GCCACTGGGA GTGGATCGGA
    GGTGAACCTG TTGCTTTTAC CAACTGGAGG AGAGGGGCCC CTCTGCACCC AAAACCTGGA
    AAGAACTGTG CTTTGGTTCA GAAAAGAGGA CAATGGCAGA CAAAGAACTG TAGCAAAGGC
    AAGGCTCACA ATTTTGTGTG CTCAAGGAAA CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13359
    Clone ID Related Accession (Same CDS sequence) XM_006538013.3 , XM_006538013.4 , XM_006538014.2 , XM_006538014.3 , XM_006538015.2 , NM_001198811.2 , XM_006538015.1 , XM_011250055.3 , NM_001198811.1 , XM_011250055.2
    Accession Version XM_006538013.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538013.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006538076.1
    CDS980..7498
    Translation

    Target ORF information:

    RefSeq Version XM_006538013.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006538013.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13359
    Clone ID Related Accession (Same CDS sequence) XM_006538013.3 , XM_006538013.4 , XM_006538014.2 , XM_006538014.3 , XM_006538015.2 , NM_001198811.2 , XM_006538015.1 , XM_011250055.3 , NM_001198811.1 , XM_011250055.2
    Accession Version XM_006538014.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006538014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006538077.1
    CDS1658..8176
    Translation

    Target ORF information:

    RefSeq Version XM_006538014.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006538014.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13359
    Clone ID Related Accession (Same CDS sequence) XM_006538013.3 , XM_006538013.4 , XM_006538014.2 , XM_006538014.3 , XM_006538015.2 , NM_001198811.2 , XM_006538015.1 , XM_011250055.3 , NM_001198811.1 , XM_011250055.2
    Accession Version XM_006538015.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006538078.1
    CDS313..6831
    Translation

    Target ORF information:

    RefSeq Version XM_006538015.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006538015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13359
    Clone ID Related Accession (Same CDS sequence) XM_006538013.3 , XM_006538013.4 , XM_006538014.2 , XM_006538014.3 , XM_006538015.2 , NM_001198811.2 , XM_006538015.1 , XM_011250055.3 , NM_001198811.1 , XM_011250055.2
    Accession Version XM_011250055.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011248357.1
    CDS1092..7610
    Translation

    Target ORF information:

    RefSeq Version XM_011250055.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Fras1 related extracellular matrix protein 1 (Frem1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011250055.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCACTCTC CAGGCTGCAC TGGGCCCAAG GCTCAGTGGT TCTTGCTGCT CCAGCTGCTT 
    CTGCTGCACC TGGACCGGGT CAGTGCCACC TTCATCAGCA TCAACCGGGG CCTGAGGGTG
    ATGAAGGGTA GTTCTGCCTT CCTGTCTGGA GATCACCTGA GGGTTGCAGT CCCTAAAGAA
    AAGGACGCCT GCAGACTGGA GGTGGTGATG AACGAGCCCG TAACCCAGAG GGTTGGGAAA
    CTCTCTCCAC AGGTCTTTGA TTGCCATTTT CTTCCCAACG AAGTGAAGTA TGTTCACAAT
    GGATGCCCAA TTCTGGATGA AGACTCTGTG AAGCTTAGAC TTTACAGGTT TACTGAAACA
    GACACCTTCA TGGAAACCTT TCTCTTGCGG GTTTATCTTG TGGAACCAGA CTGTAACATC
    ATCCGTATGA GCAGTAATGT GTTGGAAGTG ACTGAATTCT ATGGCTTATC GCAAGCCATT
    GATAAGAACC TGCTCCAGTT TGATTATGAT AGGACTGCCA GCCTGGATTG CACCATCAGA
    CTAGACCCAC TGAGGACTCA GCTGCCAGCT CACGGCAAGC TGGTCGTGGT GAATCGTAAG
    TCAGAGGGGC CTCGTGGAGA TCAGCCACAC AGCTTCTTCT CTGAGACAGA GCTAGGGGCT
    GGACTGAAGT GTCCAGATGG AAGCTGTGCC CTGGAATTGA AGCAAGTGGC AAGTCTGAAA
    GTGAGCTGTG AGGAGTTTCT GCTGACGGGG TTTCACTACC AACACATGCA GCCCCCTTCA
    CCCAACATTG ATTACATCCC CATTCAGTTG GATCTCACCG ACAGAAGGAG CAAAACCGTA
    TACAAGTCAG AGAGTGCGTG GTTGCCTGTC TATATCAGAG TTGGCATTCC TAACCAGGTC
    CCAAGGGCTG CATTTATGGC CATGTTTGTT CTGGAGGTGG ACCAGTTCAT ACTGACCCCT
    CTGACAACCT CCGTTCTGGA CTGTGAGGAG GATGAGACTC CGAAACCCTT GTTGGTGTTC
    AACATCACTA AAGCCCCACT CCAGGGCTAT GTGACCCACC TGCTGGATCA TACCAGACCC
    ATCTCTTCAT TCACCTGGAA GGATCTCAGT GACATGCAAG TTGCCTACCA GCCACCAAAC
    AGCAGCCACC CTGAGAGGAG ACATTACACG ATGGAGCTGG AGGTGTACGA CTTCTTCTTT
    GAGAGGAGTG CCCCCATCAC TGTCCACATC TCCATCAGAA CAGCAGACAC GAATGCACCA
    CGGGTGTCTT GGAATACAGG TTTGAATCTC CTAGAGGGGC AGTCTCGGGC CATCACTTGG
    GAACAATTTC AGATCGTTGA CAATGATGAC ATTGGTGCTG TGCAGTTGGT CACCATTGGT
    GGCCTGCAGC ATGGACGGCT TACAGTACGA GAGGGGAAAG GATTTCTCTT CACCGTGACT
    GACCTTCAAG CTGGAGTTGT CCGCTATCAT CACGATGACA GTGATACCAC CAAAGACTTT
    GTGGCTTTCA GGATATTCGA TGGCCACCAC AGCAGCCACC ACAAGTTTCC TATCAACATC
    TTGCCTAAAG ATGACAGCCC CCCATTCCTC ATCACCAATG TCGTGATTGA ACTAGAAGAG
    GGGAAGACCA TCTTGATCCA AGGGTCTATG TTGAGAGCTT CAGATATGGA CTCCAGTGAT
    GACTACATCT TCTTTAATAT CACAAAGTTT CCACAGGCTG GGGAGATCAT GAAGAAGCCA
    GGGCCAAGAC TGATAGGGTA TCCTGTCCCT GGCTTCCTGC AGAGAGACCT GTTCAGTGGA
    ATCATTTACT ATCGTCACTT TGGTGGAGAA ATCTTTGAAG ATTCCTTTGA ATTCGTACTG
    TGGGACAGCC ATGAGCCTCC AAATCTCTCA GTGCCACAGG TGGTGACAAT TCATATCACT
    CCAGTAGATG ACCAGCTTCC TAAAGAAGCT CCTGGCATTT CCCGGCATTT GGTTGTCAAG
    GAAACAGAGG TGGCCTACAT CACTAAAAAG CACCTACATT TCCTAGACAT GGAATCCCGT
    GATGGGGAAC TCATCTACAC AGTAACACGC CCTCCATGTT TCTCCTTCAG CCACAGACAC
    TTGGATGCTG GGAAGCTATT CATGGTGGAC AGCATACCAA AGCTAACCAA AAATCCGACA
    GCCCCAGGGC TAAGTTCATT TACTCAGCAT GCCGTGAACC ATATGAAAGT GGCCTACATG
    CCACCCATGC AAGATATAGG CCCCTCCCCT CGACATGTGC AGTTCACAGT GTCTGTCAGT
    AACCAGCATG GAGGTGCTTT GCATGGAATC TGCTTCAACA TCACAGTTCT CCCAGTGGAC
    AACCAGGTTC CCGAGGTATT CACCAACACC CTGAGAGTGG TCGAGGGCGG TCAGTGCACC
    ATCAGCACAG AGCACATTTT GGTTTCTGAT GTGGACACCC CGCTAGACAG CATCAGCCTC
    TCTCTTAAGG AGAGGCCTCT GCATGGCGGG GTAGAGCTGG ACGGATTTCC TCTAAACCCA
    AGAGGCACAT TTTCTTGGCG AGACCTCAAT ACCTTAAAAG TTTGGTATCA ACATGATGGA
    TCTGAGGTTC TTCAGGATGA GATATTTTTG GAGGTCACAG ATGGCACAAA TTCAGCAGCA
    TTTGTTCTCC ACATTGAGGT GTTCCCTGTG AATGATGAGC CACCCATTCT GAAGGCTGAC
    CTCATCCCCA TGATGCACTG CTCTGAGGGT GGAGAGGTGA CCATCACCCC TGAATACATT
    TCCGCTACTG ATGCAGACAG CGATGACTTA GAACTGCTGT TTCTGATTGC CCGAGAGCCT
    CAGCATGGAG TGGTGAGAAA GGCTGGACTT CACGTGGACA GGTTCTCTCA GGGAGATGTC
    ATCTCAGGGG CTGTGACATA CAAACATACA GGTGGCGAGA TTGGCCTTGA ACCTTGTTCT
    GACACTGTTG TATTGGTGGT TTCGGATGGT GAAGCTGACC CTTTGATGAA CGGCTGTTGC
    TACGATGGAC CTGATTCATC TGTTCCTCTT CATAAGTCCT TCCCCACATA CCAGCTCAAC
    ATCACAGTAC ATCCAGTCGA CAACCAGCCA CCTTCAATTA TAATAGGTAG GATGCTCACA
    GTGGATGAAG GCTTCTCAGC AGCCCTTACT ACTCATCACT TGACTGTTGT TGATTGGGAC
    ACTGCCCCGG ATGACTTAAA ATTCATGTTG GCTTCTCAGC CCCAGTTTGG CTACTTAGAA
    AACGCACTGC CTTCTGCAGG CTTTGAGAAA AGTAACATCG GCATAAGGAT AGCCTCATTC
    CAGTGGACAG ACATGAAGGC TTCACACATT AACTATGTGC AGTCCAGGCA CCTGAGAGTG
    GAACCAACTG CTGACCAGTT CACAGTATAT GCCACAGACG GGAAGCATCG CTCCTTGGAG
    ACAACATTTC ACGTTATCAT CAACCCCACA AACGATGAAG CTCCTGACCT TGCAGTACAG
    AACATCACTG TGTATGAGGG GCACATGGTA GAACTGGATT CTTCCATCAT CAGTGCTACT
    GACAGGGATA TTCCCAAGGA CCCCTTGCTC TTCAGCATCG CTCTCAAGCC CCAGCACGGC
    CTGCTCGTCG ATGCTGCCAT CAGCAAGGAC TCCCATCAGA TCAAGCAGCT GCAACACGAA
    ATCCACAGCT TCTCCGTGGA CCTTCTCAAG AATGGAATGA AGCTGGTCTA TGCCCACGAC
    GACTCAGAGA GCTCTGCTGA TAACTTCGTG ATCCAGTTGT CAGACGGGAA ACATAAAATC
    CTTAAAACCA TCTCAGTAAA CATTACCCCA GTGAATGACG AGACACCAAC GCTAAGCAAG
    AAGGCTGAAA TCTCAATGGC TGTGGGCGAT ACTCGCGTCC TGTCTAGTGC TGTTCTTTCA
    GCCACAGATA AAGACTCGCC CAGAGAGAAG ATTCACTATG TATTTGAAAG ACTTCCCCAA
    AATGGACAAC TTCAACTTAA GATAGGGAGG GACTGGGTCC CTCTCTCCAC TGGCATGCAG
    TGCACCCAGG AGGATGTGGA CCTGAACTTG CTAAGATACA CGCATGCTGG TAAAACGGAC
    TCCCAGGATG GAGACAGCTT CACCTTCTAC CTCTGGGATG AGGACAACAG GTCACCTGCA
    TTTGACTGTC ACATCATCAT TGAAGACATG GGCAAAGGTG ATATTGTCAT TCATGCAAAA
    CCACTCGTGG TAGTCAAAGG TGACAGAGGT CTCTTGACGA CTGCCACCCT TCTGGCTGTG
    GATGGAGCAG ACAAGCCTGA GGAACTGCTC TACCTCATCA CCTCCCCACC ACGGCATGGC
    CAAGTGGAGT ACGTCCACTC TCCTGGAGTC CCCATTGCCA GCTTCAGCCA AATGGACATA
    GCAGGACAGA CAGTGTGCTA CATACACAAG AGCAGGACAG CTGTCCCCAC TGACAGCTTC
    AGATTTACCA TCAGCAATGG ACTGCAGACC CAGCGTGGGG TGTTTGAAAT CACACTGCAG
    ACTGTGGACA GCGCCTTGCC TGTGCTGACC AAGAACAAAA GGCTGAGGCT AGCCGAAGGG
    GCCATGGGCC TCCTGTCCGC TGATCACCTT CAGCTGACTG ACCCCGACAC ACCTCCAGAG
    AACCTGACCT TCTTTCTGGC TCAGCTCCCA CGCCACGGGT ACCTCTTCCT GAGAGGGAAA
    GCACTCCAAC ACAACTTCAC CCAGCGAGAT GTGGACAGCG GGGGCGTGGC CTACCAGCAC
    TCAGGAGGCG GCGCCCGGGA GGACTATTTT ACTTTTCTAG CCACAGACAG GAAGAACCAA
    GGCTTTGTTG TGGATGGGAA AGTTCAGAAA GAGCCTGTTC GCTTCACAAT CCAGGTGGAT
    CAGCTGGACA AGGCAGCGCC CCGTATCACA CACTTGCACT CCCCTACTCA AGTGGGGCTC
    TTGAAAAATG GCTGCTATGG GATTTACATC ACTTCCCGTG TGCTGAAGGC ATCAGACCCT
    GACACAGAGG ATGACCAGAT CATCTTTAAG ATTTTACGAG GCCCATTGTA CGGACGTCTG
    GAGAACACAA CAACAGGTGA ATTCATCCAC GAGCGATTTA GCCAGAAGGA CTTAAGCCAT
    AAGACCATCC TTTACATCAT AAACCCTTCT TTGCAAGTGA CTTCCGATAT CCTGGAATTT
    CAGGCCATGG ACCCCACAGG GAACACTGCC ACTCCCCAGA GCTTGGACTT GAGATGGTCG
    TATATTGAAT GGGCCCAGAC TGCATATGAG GTTTGTGAGA ATGTGGGCTT ATTGCCCTTG
    GAAGTTACCA GAAGGGGATA TCCCATGGAC TCAGCCTTTG TGGGTGTAGA GGTTAACCAA
    GTGTCAGCTA CAGTTGGAAA AGATTTTACT GTCACTCCAT CCAAACTGCT TCAATTTGAC
    CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT
    GATGAGGTCT TTGAAGTAAT TCTGAACTCC CCTGTGAATG CGGTTCTTGG CACGCAGACA
    AAAGCGGCGG TGAAAATTTT GGACTCAAAA GGAGGACGGT GCCATCCTTC AAATTCCTTC
    AACCAAAGCA AGCACAGCAC GTGGGGGAAG GGCCCTTGGC ATCCGCTGCC CTCAGGCTCA
    TCCTCGCTCA CCACTTCAGG TTCTCCTCTT CTGGAAAGGC CTCCTCCATC TTTCACCAGT
    GGAGATGCTC TTCAGGGTTT TGGTCTCACC GACCTCACTC AAAGGAAGAC AATGACCCAG
    GGGAATGGCA AATCAGTTCT CCCATCCTCT GTTTGTAGAA ATGGGACAGA CACCATCTAC
    AATTACCATG GTATAGTTTC CTTGAAACTA GAGGGCGACA GATTCTCAGC TCACAAACGG
    AAGGCCAAAA TATCCATTGT TAGTCAGCCA CAGAGGACAA TCAAGGTGGC AGAGCTGCCT
    CTAGCTGATA AGGTGGAATC CACAACTGAT TTGCACTTTC TCAGACAGGG TCTGCGGCCC
    TTGTTTCCAA AGAATTGTTC TGTGGACTTA AAGGGACTCT TTCATTTTGA AGAAAGCACC
    CACAGATTGT ATCAATGTGA TGGGATCTCC TGGAAAGCCT GGAGCCCCCA AACCAAGGGG
    CTGGAGGACA GATCCTGCCC AGGTGGATGG CTCCTTCATT CTGGCTACTG TCACATCTTG
    GTCACAAGAC AGAAAGGCAC CTGGACCACA GCTACCCGAG CTTGCAGAGA ACAGCACCAG
    GGCGACCTTG TGACCGTACT CTCCAGAAGG CATATGCAGT GGCTATGGGC GATGAGTGGA
    AGAAAACCCT TCTGGATAGG CTTGAAGAAC CAACCGAGGA CTGGCCACTG GGAGTGGATC
    GGAGGTGAAC CTGTTGCTTT TACCAACTGG AGGAGAGGGG CCCCTCTGCA CCCAAAACCT
    GGAAAGAACT GTGCTTTGGT TCAGAAAAGA GGACAATGGC AGACAAAGAA CTGTAGCAAA
    GGCAAGGCTC ACAATTTTGT GTGCTCAAGG AAACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.