Mast4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mast4
Entrez Gene ID	328329
Full Name	microtubule associated serine/threonine kinase family member 4
Synonyms	4930420O11Rik,AI642422,mKIAA0303
General protein information	Preferred Names microtubule associated serine/threonine kinase family member 4 Names microtubule-associated serine/threonine-protein kinase 4 mast4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 D1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006517701.3	XP_006517764.1	microtubule-associated serine/threonine-protein kinase 4 isoform X7
XM_006517703.4	XP_006517766.1	microtubule-associated serine/threonine-protein kinase 4 isoform X13
XM_006517704.4	XP_006517767.1	microtubule-associated serine/threonine-protein kinase 4 isoform X15
XM_006517706.3	XP_006517769.1	microtubule-associated serine/threonine-protein kinase 4 isoform X17
XM_011244674.3	XP_011242976.1	microtubule-associated serine/threonine-protein kinase 4 isoform X20
XM_017315531.1	XP_017171020.1	microtubule-associated serine/threonine-protein kinase 4 isoform X5
XM_030247308.1	XP_030103168.1	microtubule-associated serine/threonine-protein kinase 4 isoform X10
XM_006517702.3	XP_006517765.1	microtubule-associated serine/threonine-protein kinase 4 isoform X9
XM_030247309.1	XP_030103169.1	microtubule-associated serine/threonine-protein kinase 4 isoform X12
XM_006517705.4	XP_006517768.1	microtubule-associated serine/threonine-protein kinase 4 isoform X16
XM_030247307.1	XP_030103167.1	microtubule-associated serine/threonine-protein kinase 4 isoform X8
XM_006517707.3	XP_006517770.1	microtubule-associated serine/threonine-protein kinase 4 isoform X19
XM_017315527.1	XP_017171016.1	microtubule-associated serine/threonine-protein kinase 4 isoform X1
XM_017315532.1	XP_017171021.1	microtubule-associated serine/threonine-protein kinase 4 isoform X6
NM_175171.3	NP_780380.2	microtubule-associated serine/threonine-protein kinase 4
XM_006517703.3	XP_006517766.1	microtubule-associated serine/threonine-protein kinase 4 isoform X10
XM_011244673.2	XP_011242975.1	microtubule-associated serine/threonine-protein kinase 4 isoform X11
XM_006517704.3	XP_006517767.1	microtubule-associated serine/threonine-protein kinase 4 isoform X12
XM_006517705.3	XP_006517768.1	microtubule-associated serine/threonine-protein kinase 4 isoform X13
XM_011244673.3	XP_011242975.1	microtubule-associated serine/threonine-protein kinase 4 isoform X14
XM_030247310.1	XP_030103170.1	microtubule-associated serine/threonine-protein kinase 4 isoform X18
XM_017315530.1	XP_017171019.1	microtubule-associated serine/threonine-protein kinase 4 isoform X4
XM_006517701.4	XP_006517764.1	microtubule-associated serine/threonine-protein kinase 4 isoform X7
XM_011244674.2	XP_011242976.1	microtubule-associated serine/threonine-protein kinase 4 isoform X16
XM_006517702.4	XP_006517765.1	microtubule-associated serine/threonine-protein kinase 4 isoform X11
XM_017315528.1	XP_017171017.1	microtubule-associated serine/threonine-protein kinase 4 isoform X2
XM_017315529.1	XP_017171018.1	microtubule-associated serine/threonine-protein kinase 4 isoform X3
XM_011244672.2	XP_011242974.1	microtubule-associated serine/threonine-protein kinase 4 isoform X9

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAST4	NP_001158136.1
Bos taurus (cattle)	MAST4	XP_003587555.2
Rattus norvegicus (Norway rat)	Mast4	XP_006231936.1
Gallus gallus (chicken)	MAST4	XP_004937359.1
Pan troglodytes (chimpanzee)	MAST4	XP_003950594.1
Mus musculus (house mouse)	Mast4	NP_780380.2
Canis lupus familiaris (dog)	MAST4	XP_005617968.1

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of Presenilin 1 and 2 interacting proteins in mouse cerebral cortex during development.
Kumar A, Thakur MK
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience38138-46(2014 Nov)

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Cloning of the genes encoding two murine and human cochlear unconventional type I myosins.
Crozet F, el Amraoui A, Blanchard S, Lenoir M, Ripoll C, Vago P, Hamel C, Fizames C, Levi-Acobas F, Dep?tris D, Mattei MG, Weil D, Pujol R, Petit C
Genomics40(2)332-41(1997 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mast4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mast4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu74760	XM_011244672.2 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1301.30 $1859.00
OMu49077	XM_006517701.4 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu49078	XM_006517702.4 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu49079	XM_006517703.4 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu49080	XM_006517704.4 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu49081	XM_006517705.4 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu74761	XM_011244673.3 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu74762	XM_011244674.3 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu86254	XM_030247307.1 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu86255	XM_030247308.1 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu86256	XM_030247309.1 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu86257	XM_030247310.1 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu49082	XM_006517706.3 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu49083	XM_006517707.3 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu04956	NM_175171.3 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OMu49069	XM_017315527.1 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OMu49070	XM_017315528.1 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OMu49071	XM_017315529.1 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OMu49073	XM_017315531.1 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OMu49074	XM_017315532.1 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OMu78478	XM_017315530.1 Latest version!	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
View Old Accession Versions >>
OMu49079	XM_006517703.3	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu49080	XM_006517704.3	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu49081	XM_006517705.3	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu74761	XM_011244673.2	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1301.30 $1859.00
OMu49077	XM_006517701.3	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu74762	XM_011244674.2	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1301.30 $1859.00
OMu49078	XM_006517702.3	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mast4 ( XM_011244672.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242974.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu74760
Clone ID Related Accession (Same CDS sequence)	XM_011244672.2
Accession Version	XM_011244672.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244672.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCCC AGCGGGAAAG ACTACAGATT CCGGGGCTGA CCTTGGATCT CACTCCAAGG 
AGCCTGAGTC CCACCCCATC CAGCCCGGGC AGCCCCTGTA GTCCTCTCTT GACCTTTCAC 
TTCTGGAGTT GCCGAACAAG CAACCGGAAG AGTTTGATAG GCAATGGCCA GTCTCCAGCA 
CTGCCTCGAC CACACTCACC TCTCTCTGCT CATGCAGGAA ATAGCCCTCA AGACAGTCCA 
AGGAATTTCT CCCCCAGTGC CTCAGCCCAT TTCTCATTTG CGAGGAGAAA TGACAGAACG 
GATGGGCGCC GCTGGTCTCT GGCTTCTCTC CCTTCCTCAG GCTATGGGAC AAATACCCCA 
AGCTCCACCG TCTCCTCATC CTGTTCTTCC CAGGAGAAGT TGCACCAGCT ACCATACCAG 
CCAACCCCAG ATGAATTACA CTTCTTATCC AAACACTTCT GCACAACAGA AAGTATCGCC 
ACTGAGAACC GGTGCAGAAA CACACCCATG CGTCCACGTT CCCGGAGTCT CAGCCCTGGA 
CGGTCCCCCG CCTGCTGTGA CCATGAAATA ATTATGATGA ACCATGTCTA CAAAGAAAGG 
TTCCCAAAGG CCACAGCTCA GATGGAAGAG CGTCTGAAGG AGATCATCAC CAGCTACTCT 
CCAGACCATG TTCTCCCCTT GGCAGATGGG GTACTTAGTT TCACTCACCA TCAGATCATT 
GAGCTGGCTC GGGACTGTTT GGATAAATCT CACCAGGGTC TCATCACGTC GAGATACTTT 
TTTGAGTTGC AGCACAAACT GGACAAGTTG CTCCAGGAGG CTCACGATCG CTCTGAAAGT 
GGAGAACTGG CATTTATCAA GCAACTAGTC CGAAAGATCC TAATTGTCAT TGCCCGCCCC 
GCTCGGTTAT TGGAGTGTTT GGAATTTGAT CCTGAAGAAT TTTATTACCT ATTGGAAGCT 
GCAGAAGGCC ATGCCAAAGA AGGCCAAGGA ATCAAAACTG ACATCCCTAG GTATATCATC 
AGCCAGCTGG GACTCAATAA GGACCCTTTA GAAGAAATGG CTCAGTTGGG GAATTACGAC 
AGTAGGACAG CAGAGACACC AGAGATGGAT GAGTCAGTGA GTAGCTCAAA TACTTCCCTG 
AGACTTCGAA GGAAACCCCG AGAGAGTGAT TTTGAAACAA TTAAATTGAT CAGCAATGGA 
GCCTACGGGG CAGTCTACTT TGTTCGGCAC AAAGAGTCCC GCCAGAGGTT TGCCATGAAG 
AAGATCAACA AGCAGAACCT CATCCTTCGG AACCAGATCC AGCAGGCCTT CGTGGAGCGA 
GACATCCTGA CTTTCGCAGA GAACCCCTTT GTGGTCAGCA TGTATTGCTC CTTTGAAACG 
AGGCGTCACT TATGCATGGT CATGGAGTAT GTAGAAGGGG GAGACTGTGC GACCCTAATG 
AAAAACATGG GACCTCTCCC AGTTGATATG GCCAGAATGT ATTTCGCCGA GACCGTCTTG 
GCCTTGGAGT ACCTGCATAA TTACGGAATC GTACACAGGG ACTTGAAGCC AGACAACCTG 
TTGGTCACCT CCATGGGGCA CATAAAACTG ACTGACTTCG GCTTGTCTAA GGTGGGATTA 
ATGAGCATGA CCACCAATCT CTATGAAGGC CACATAGAGA AGGACGCTCG AGAGTTCTTA 
GATAAACAGG TCTGTGGTAC ACCTGAGTAC ATTGCCCCCG AGGTGATTCT GAGACAGGGC 
TACGGGAAAC CCGTGGACTG GTGGGCCATG GGCATTATCC TCTATGAATT TCTGGTCGGA 
TGTGTGCCTT TCTTTGGGGA CACTCCAGAA GAGCTATTTG GACAAGTCAT CAGTGATGAA 
ATCAACTGGC CTGAAAAGGA CGAGGCCCCT CCTCCAGACG CTCAGGAGCT GATTACCTTG 
CTCCTCAGGC AGAATCCGCT GGAGAGGCTG GGAACAGGTG GAGCCTATGA AGTGAAGCAG 
CATCGTTTCT TCCGCTCCTT AGACTGGAAC AGTTTGCTGA GACAGAAGGC GGAATTTATT 
CCCCAACTGG AATCGGAGGA TGACACAAGT TATTTTGATA CTCGGTCAGA GAAGTATCAT 
CACATGGAGA CGGAGGAGGA GGACGATACA AACGATGAGG ACTTCACCGT GGAGATAAGG 
CAGTTTTCTT CCTGTTCACA CAGGTTTTCA AAAGTTTTCA GCAGTATAGA TCGCATAACT 
CAAAATTCAG GAGAAGACAA AGATGACTCT GAGGACAAGA CCAAAAGCAC AACGTTGCCA 
TCCACAGAGA CACTCAGCTG GAGTTCCGAA TACTCTGAAA TGCAACAGTT ATCGACCTCC 
AACTCTTCAG ATACTGAAAG CAACAGGTGC AAACTCAGCT CTGGCTTGCT CCCCAAGCTG 
GCTATTTCGA CAGATGGGGA ACAAGATGAG GCTGTCCCTT GCTCTGGAGA CCCCAGAGAG 
GAGCCAGAGA AACCTGTCCC TCCCTCTGAG GAGTGTACTC AGGAGGAGCC CGAGGTCACC 
ACCCCAGCCA GCACCATCAG CAGTTCCACA CTGTCAGTTG GCAGTTTTTC AGAGCACTTG 
GATCAGATAA ATGGGCGAAG CGAGTGTGTG GACAGTACAG ATAATTCCTC AAAGCCATCC 
AGTGAACCCA CTTCTCACGT GGCTCGACAG CGCTTAGAAA GCACAGAGAA AAAGAAAATT 
TCTGGGAAAG TCACAAAGTC CCTCTCGGCC AGTGCTCTGT CCCTCATGAT CCCAGGAGAT 
ATGTTCGCTG TATCTCCATT GGGAAGCCCA ATGTCCCCAC ACTCCCTGTC TTCAGACCCT 
TCTTCTTCAC GGGATTCCTC TCCCAGCCGA GACTCTTCTG CAGCATCTGC CAGTCCGCAT 
CAGCCCATTG TCATCCACAG CTCAGGCAAG AACTATGGGT TCACCATCCG TGCTATCCGC 
GTGTACGTGG GGGACAGTGA CATCTACACA GTGCACCATA TCGTCTGGAA CGTAGAAGAA 
GGAAGTCCCG CATACCAGGC AGGACTGAAG GCCGGGGATC TGATCACACA CATCAACGGA 
GAGCCGGTGC ACGGCCTCGT CCACACGGAA GTTATCGAGC TCCTGCTGAA GAGTGGGAAT 
AAGGTGTCTA TCACCACTAC TCCATTTGAA AACACATCAA TCAAAACGGG ACCAGCCAGG 
AGAAACAGTT ACAAGGGCCG GATGGTGAGA CGAAGCAAGA AGTCCAAGAA GAAGGAGAGT 
CTAGAAAGGA GGAGATCTCT CTTCAAGAAG CTGGCCAAGC AGCCTTCTCC TTTGCTCCAC 
ACCAGCCGAA GTTTCTCCTG CTTAAACCGG TCCCTGTCAT CTGGAGAGAG CCTCCCGGGT 
TCCCCAACTC ACAGCTTGTC CCCGAGGTCT CCAACACCCA GTTATCGTTC TACTCCCGAT 
TTTCCGTCAG GTACAAATTC CTCCCAGAGC AGCTCCCCAA GTTCAAGTGC CCCCAATTCT 
CCAGCAGGTT CAGGGCACAT CCGGCCCAGC ACCCTCCATG GCCTGGCTCC CAAACTCAGC 
GGGCAGCGAT ACCGCTCTGG AAGACGGAAG TCGGCTGGCA GCATCCCTCT CTCCCCGCTG 
GCCAGGACAC CCTCTCCCAC TCCACAGCCT ACCTCTCCTC AGCGTTCACC ATCCCCACTG 
TTGGGACACT CACTGGGCAA TGCCAAGATC ACTCAGGCCT TTCCTAGCAA GATGCACTCT 
CCCCCCACAA TCGTCAGACA CATCGTGAGG CCCAAGAGTG CAGAGCCGCC CCGCTCCCCA 
CTGCTGAAAC GGGTGCAGTC GGAGGAAAAG TTGTCACCCT CCTATGGCAG TGACAAGAAG 
CTTCTGTGCT CCCGCAAGCA TAGCCTAGAG GTGACACAAG AGGAGGTACA GAGGGAGCAG 
TGTCAGCGGG AAGTGACACT GCAGAGCCTG GAAGAGAATG TGTGTGACGC TCCTTCCCTC 
AGTCGGGCCA GGCCAGTGGA GCAAGGCTGT CTGAAACGCC CCGTGTCCCG GAAGGTGGGC 
AGGCAAGAGT CTGTGGATGA CCTGGACCGG GACAAGCTGA AAGCCAAGGT TGTCGTAAAG 
AAACCAGAAG AGAAACATGA ATCGCACCAG AAACCTCACA GCCTTGGTGG TGATTCGGAA 
AGCTATGCTC TCTTCAGGCT AGAGGAGAGA GAGAAAAAAG TGTACTCCAA GGGGTTGGAA 
AGGTCAGGCC ATTTTGAAAA CACATCAGCA GAGTTGCCTT CTGTGGGCAG CCTGCTGAAG 
GACACTCTTC ACAAGCAGGC CAGCGTGAGG GCCAGCGAGG GGGTGACCTC AGACGGGGCA 
GCTTGCAGCC TGACACCAGG GGAGCACAGC CAGTCTCTAG GTGACTTTAA GCGGGCCTCA 
GCTTCTGGCA TTCTCCATGA TAGTGTGTGC CCCATCTCTG ATAGGCCTGC TCCTGGAAAG 
GTTGAATACT CGGAGAAGGC CTCTCAGGCC AAAGAGCTCC TTCGAAGTGA AAAACTAGAC 
AGCAAGCTGG CCAATATTGA TTACCTCAGA AAGAAAATGT CATTGGATGA CAAAGATGAC 
AGCCACTGTG CCATCCTGAA ACCCAAGATA ACATCTAGCG CCCATGAATG TCTGCCAGGG 
AACCCCATAC GGCCCATGGC AGGGCAACAA GAGACCCCGC CAGCCTCTGA AAACCGGGCA 
TTCATCAACA GTACCCACAC ACCTCAGATG AGTGCAGTTT CCTTTGTTCC TCTCAAAGCC 
TTAGCTGGCC GGGTAGAGAA CGGAGGGGAG AAAGCAGGCT TAGCTGCTCC CGAGTCCCCT 
GTCAGGAAGA GCCCCTCCGA GTATAAGCTA GAGGGCAGGT CAGTTTCATG TCTCAAGCCG 
ATCGAGGGCA CACTGGACAT TGCTCTCCTG TCTGGACCTC ACGCCTCCAA AACAGAGTTG 
CTTTCCCCAG AGCCTGCACA GAGTCCCAGC CCAGGCATCA ACGTGGGACC ATGTGTGCCA 
CTAGCTCTTC CTGGGAGCAG TGGGAAAAAG GGAGACTCCA CCAGCCTGAG AGAGCCTTCC 
TCAGCCAACT TAAAAGTAAA TAAATCTTAT CTGCTGGAGC CTCGGTTCCT ACCCCCGAGC 
CGGGCTCTCC AGGACTCTCT CGCAGCCTCT GGGCCAGAAC CGAAGTCAAA GCCGGAAAGG 
AAGCTCATTC ATCCTTCTGC CAGGAGCCCA GCAACTGTCA CAGAGAGCAA TCTTCAGCAG 
AAAGAGGGTG GTCCCGCCAC ACACCAAGAC CGCTCCACTG ACACCAGGAA CCTCCCTGGC 
CCAGGGCAGA CCCTACACAA TGTGGACCTA CCCAGGCTGT GTACACGTGC CCCACTCCCA 
CCGGAAGGGA CGCCCGCAAA GGAGAAGCCA TGTCTGAAGG AACCCTCTGC CAAGGTGAAA 
AGCGAGTGGT CTGCCGTGAG GGATGACGGA CACAGAGATC CCTGTGCGAA GCTGTGCCCG 
GCAGAGACTG GTAAAGCCAG CGACAGTTCC AAACCCCTGC CTTCCGGGGG GAGGACCCAA 
CCCGATTTCT ACAAGCAGAC CCAGACTTCG GAGAAAGCAT GGGCGCATGC AAAAACAAAC 
CACAAAGATA GCCAAGATGA GGTGAAGTCC CTGGCCAGGG AGGACTCAGC TTCACTTTTA 
TATGAAAAGG AGATAGGCCG GGCACGAAAA GGTCCTGAAC CCAAACCGGA AGTTCCTGCT 
ACCCGGTGCC CTCCTCAGCC ACCAGGAATT GAGGGTGAGA AGCGAGAAAA GCTCTCCGCT 
GCCCCCTCTT TGCAGAAACA GGCTCCCAAA GAGCCAGACA GGAAGGAACA GACTTCGCAA 
AGGCCTGGAG GTAGTGGCCC TCAACAACCC CCACCCACCA AAGAGCTGTC TAACTCAGCA 
TCCTGGCAGC ACGGCAGTTC TCCGAGTCAC ACTTTAAAGA AGGAGCCCGG GACCAAAGCT 
GCCGCTGCAG AACCAAGCAC CAGCCTTCAT GACACTCCCC GATCTGCTAC AGCCACCACC 
ACTGCCATTG CCACCACCAC CACTACCACC AGTGCCGGGC ACAGTGACTG CAGTAGCCAT 
AAGGCCCGGC CTGGCCCTGA CCCCAGCCCT TCAAAGTCTA AGCACCAAGA CAGGTCCCTC 
TCCTCACAGA AGCTGAGTGC TGGCTCTGCA AAAGGCAAAG AGCCTGTCAC TCAACCCCTG 
GGTGGTTCCA TCAGAGAAGG CAAGGGTGGC AGCAAGGGTC CAGTGGACAC ATTTTCTGCT 
GTCCTGACCA CCCAGGGCAA AGCAAGTGAT GTGCTTGTGC AGGGAGAAGG TCGGGTCTCA 
ATCATTGTCC ACACTGAAGA GTGTCCTCTC GATGCCAAAC TGAAAAACAC CAATGGAGGG 
TGTCCCCCAG AGATGCAGGC GAAGCATCCA CCCAGACAAG GACATCTCAG TGAAGCAGCA 
GACCAGAAGC CACTCATTGC TGGTGAGAAG CAAAGCCCGT CTCCAAAGCA TCCCAAACCA 
TCCACTGTGA AAGATTACCC CAGTCTGTGC AGACAGACAG ACAGAAGCCC AAGCCATCAG 
GCTACCACTG GGGACAGGAA GGCAGAAGGA AAGAAATGCA CAGACGCACT TTATGTCGCA 
GCCCCAGAGG GCTACAAGCC AGAGGCCAGC CCTTCTCTCC ACCACGGCGA GACCGGACTC 
AGAGGCTCAG AGAGGCCACC CATGGGCATG GGGAAGGGCT TCTCTGAGCC CAAGGGGAAA 
GGGCCAGGTC CCCAGAAGTC ACTGGCTGAA ACAGGCAAGC CCAGCGGTAT GAAAAGGTCA 
CCCTCTGCCA CCGTGCAGAG CTCTCTCCGC TCAGCTGCCC CCCCAGAAAA GTCTCTGAGT 
TACTCAGCCA GCTTTCCCGA GGCCCAGCCT GGAGTGCGAG AGGTCCCTGC AGCCAACAGC 
AGCCCCTCAT CTGCCAAGGC TACAGGGGGG ACCTCAGAGT TCCCAGCCCC CAGCAGCAGG 
GACCACAGGA AGCTTCAGTC TGGAGGAGAC GGCCGAAGCC AAATGATAAA GAGTGACTCT 
CTGCCCTCCT TCCGCCTCTC CACCTCTGCT CTGGAGTCAC ATTTCCAGGA TCCACAGGTG 
CCCATCGCAT CAGGCCACCG AGGCAGGGCA CTGTCAGTAA CTGCTGCCAC AGGAGAACCC 
AAAGGGAGAG AGCTCGCCCA GCCTCCCCCA GTCAGGAAAC AGAATGCGTG CAGAGAGGCG 
ACCAGAGCAC CCCCAGCCCC AAGCACAGAT CGCTCCCTCC CTCTTTCCTC AGAGAAAGAC 
TTCGTGGTTC GGCAGAGAAG GGGCAAGGAG ACCTTAAGGA GCAGTCCTCA CAAAAAGGCC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242974.1
CDS	257..7582
Translation	MKAQRERLQIPGLTLDLTPRSLSPTPSSPGSPCSPLLTFHFWSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_011244672.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244672.2

ORF Insert Sequence:

ATGAAAGCCC AGCGGGAAAG ACTACAGATT CCGGGGCTGA CCTTGGATCT CACTCCAAGG 
AGCCTGAGTC CCACCCCATC CAGCCCGGGC AGCCCCTGTA GTCCTCTCTT GACCTTTCAC 
TTCTGGAGTT GCCGAACAAG CAACCGGAAG AGTTTGATAG GCAATGGCCA GTCTCCAGCA 
CTGCCTCGAC CACACTCACC TCTCTCTGCT CATGCAGGAA ATAGCCCTCA AGACAGTCCA 
AGGAATTTCT CCCCCAGTGC CTCAGCCCAT TTCTCATTTG CGAGGAGAAA TGACAGAACG 
GATGGGCGCC GCTGGTCTCT GGCTTCTCTC CCTTCCTCAG GCTATGGGAC AAATACCCCA 
AGCTCCACCG TCTCCTCATC CTGTTCTTCC CAGGAGAAGT TGCACCAGCT ACCATACCAG 
CCAACCCCAG ATGAATTACA CTTCTTATCC AAACACTTCT GCACAACAGA AAGTATCGCC 
ACTGAGAACC GGTGCAGAAA CACACCCATG CGTCCACGTT CCCGGAGTCT CAGCCCTGGA 
CGGTCCCCCG CCTGCTGTGA CCATGAAATA ATTATGATGA ACCATGTCTA CAAAGAAAGG 
TTCCCAAAGG CCACAGCTCA GATGGAAGAG CGTCTGAAGG AGATCATCAC CAGCTACTCT 
CCAGACCATG TTCTCCCCTT GGCAGATGGG GTACTTAGTT TCACTCACCA TCAGATCATT 
GAGCTGGCTC GGGACTGTTT GGATAAATCT CACCAGGGTC TCATCACGTC GAGATACTTT 
TTTGAGTTGC AGCACAAACT GGACAAGTTG CTCCAGGAGG CTCACGATCG CTCTGAAAGT 
GGAGAACTGG CATTTATCAA GCAACTAGTC CGAAAGATCC TAATTGTCAT TGCCCGCCCC 
GCTCGGTTAT TGGAGTGTTT GGAATTTGAT CCTGAAGAAT TTTATTACCT ATTGGAAGCT 
GCAGAAGGCC ATGCCAAAGA AGGCCAAGGA ATCAAAACTG ACATCCCTAG GTATATCATC 
AGCCAGCTGG GACTCAATAA GGACCCTTTA GAAGAAATGG CTCAGTTGGG GAATTACGAC 
AGTAGGACAG CAGAGACACC AGAGATGGAT GAGTCAGTGA GTAGCTCAAA TACTTCCCTG 
AGACTTCGAA GGAAACCCCG AGAGAGTGAT TTTGAAACAA TTAAATTGAT CAGCAATGGA 
GCCTACGGGG CAGTCTACTT TGTTCGGCAC AAAGAGTCCC GCCAGAGGTT TGCCATGAAG 
AAGATCAACA AGCAGAACCT CATCCTTCGG AACCAGATCC AGCAGGCCTT CGTGGAGCGA 
GACATCCTGA CTTTCGCAGA GAACCCCTTT GTGGTCAGCA TGTATTGCTC CTTTGAAACG 
AGGCGTCACT TATGCATGGT CATGGAGTAT GTAGAAGGGG GAGACTGTGC GACCCTAATG 
AAAAACATGG GACCTCTCCC AGTTGATATG GCCAGAATGT ATTTCGCCGA GACCGTCTTG 
GCCTTGGAGT ACCTGCATAA TTACGGAATC GTACACAGGG ACTTGAAGCC AGACAACCTG 
TTGGTCACCT CCATGGGGCA CATAAAACTG ACTGACTTCG GCTTGTCTAA GGTGGGATTA 
ATGAGCATGA CCACCAATCT CTATGAAGGC CACATAGAGA AGGACGCTCG AGAGTTCTTA 
GATAAACAGG TCTGTGGTAC ACCTGAGTAC ATTGCCCCCG AGGTGATTCT GAGACAGGGC 
TACGGGAAAC CCGTGGACTG GTGGGCCATG GGCATTATCC TCTATGAATT TCTGGTCGGA 
TGTGTGCCTT TCTTTGGGGA CACTCCAGAA GAGCTATTTG GACAAGTCAT CAGTGATGAA 
ATCAACTGGC CTGAAAAGGA CGAGGCCCCT CCTCCAGACG CTCAGGAGCT GATTACCTTG 
CTCCTCAGGC AGAATCCGCT GGAGAGGCTG GGAACAGGTG GAGCCTATGA AGTGAAGCAG 
CATCGTTTCT TCCGCTCCTT AGACTGGAAC AGTTTGCTGA GACAGAAGGC GGAATTTATT 
CCCCAACTGG AATCGGAGGA TGACACAAGT TATTTTGATA CTCGGTCAGA GAAGTATCAT 
CACATGGAGA CGGAGGAGGA GGACGATACA AACGATGAGG ACTTCACCGT GGAGATAAGG 
CAGTTTTCTT CCTGTTCACA CAGGTTTTCA AAAGTTTTCA GCAGTATAGA TCGCATAACT 
CAAAATTCAG GAGAAGACAA AGATGACTCT GAGGACAAGA CCAAAAGCAC AACGTTGCCA 
TCCACAGAGA CACTCAGCTG GAGTTCCGAA TACTCTGAAA TGCAACAGTT ATCGACCTCC 
AACTCTTCAG ATACTGAAAG CAACAGGTGC AAACTCAGCT CTGGCTTGCT CCCCAAGCTG 
GCTATTTCGA CAGATGGGGA ACAAGATGAG GCTGTCCCTT GCTCTGGAGA CCCCAGAGAG 
GAGCCAGAGA AACCTGTCCC TCCCTCTGAG GAGTGTACTC AGGAGGAGCC CGAGGTCACC 
ACCCCAGCCA GCACCATCAG CAGTTCCACA CTGTCAGTTG GCAGTTTTTC AGAGCACTTG 
GATCAGATAA ATGGGCGAAG CGAGTGTGTG GACAGTACAG ATAATTCCTC AAAGCCATCC 
AGTGAACCCA CTTCTCACGT GGCTCGACAG CGCTTAGAAA GCACAGAGAA AAAGAAAATT 
TCTGGGAAAG TCACAAAGTC CCTCTCGGCC AGTGCTCTGT CCCTCATGAT CCCAGGAGAT 
ATGTTCGCTG TATCTCCATT GGGAAGCCCA ATGTCCCCAC ACTCCCTGTC TTCAGACCCT 
TCTTCTTCAC GGGATTCCTC TCCCAGCCGA GACTCTTCTG CAGCATCTGC CAGTCCGCAT 
CAGCCCATTG TCATCCACAG CTCAGGCAAG AACTATGGGT TCACCATCCG TGCTATCCGC 
GTGTACGTGG GGGACAGTGA CATCTACACA GTGCACCATA TCGTCTGGAA CGTAGAAGAA 
GGAAGTCCCG CATACCAGGC AGGACTGAAG GCCGGGGATC TGATCACACA CATCAACGGA 
GAGCCGGTGC ACGGCCTCGT CCACACGGAA GTTATCGAGC TCCTGCTGAA GAGTGGGAAT 
AAGGTGTCTA TCACCACTAC TCCATTTGAA AACACATCAA TCAAAACGGG ACCAGCCAGG 
AGAAACAGTT ACAAGGGCCG GATGGTGAGA CGAAGCAAGA AGTCCAAGAA GAAGGAGAGT 
CTAGAAAGGA GGAGATCTCT CTTCAAGAAG CTGGCCAAGC AGCCTTCTCC TTTGCTCCAC 
ACCAGCCGAA GTTTCTCCTG CTTAAACCGG TCCCTGTCAT CTGGAGAGAG CCTCCCGGGT 
TCCCCAACTC ACAGCTTGTC CCCGAGGTCT CCAACACCCA GTTATCGTTC TACTCCCGAT 
TTTCCGTCAG GTACAAATTC CTCCCAGAGC AGCTCCCCAA GTTCAAGTGC CCCCAATTCT 
CCAGCAGGTT CAGGGCACAT CCGGCCCAGC ACCCTCCATG GCCTGGCTCC CAAACTCAGC 
GGGCAGCGAT ACCGCTCTGG AAGACGGAAG TCGGCTGGCA GCATCCCTCT CTCCCCGCTG 
GCCAGGACAC CCTCTCCCAC TCCACAGCCT ACCTCTCCTC AGCGTTCACC ATCCCCACTG 
TTGGGACACT CACTGGGCAA TGCCAAGATC ACTCAGGCCT TTCCTAGCAA GATGCACTCT 
CCCCCCACAA TCGTCAGACA CATCGTGAGG CCCAAGAGTG CAGAGCCGCC CCGCTCCCCA 
CTGCTGAAAC GGGTGCAGTC GGAGGAAAAG TTGTCACCCT CCTATGGCAG TGACAAGAAG 
CTTCTGTGCT CCCGCAAGCA TAGCCTAGAG GTGACACAAG AGGAGGTACA GAGGGAGCAG 
TGTCAGCGGG AAGTGACACT GCAGAGCCTG GAAGAGAATG TGTGTGACGC TCCTTCCCTC 
AGTCGGGCCA GGCCAGTGGA GCAAGGCTGT CTGAAACGCC CCGTGTCCCG GAAGGTGGGC 
AGGCAAGAGT CTGTGGATGA CCTGGACCGG GACAAGCTGA AAGCCAAGGT TGTCGTAAAG 
AAACCAGAAG AGAAACATGA ATCGCACCAG AAACCTCACA GCCTTGGTGG TGATTCGGAA 
AGCTATGCTC TCTTCAGGCT AGAGGAGAGA GAGAAAAAAG TGTACTCCAA GGGGTTGGAA 
AGGTCAGGCC ATTTTGAAAA CACATCAGCA GAGTTGCCTT CTGTGGGCAG CCTGCTGAAG 
GACACTCTTC ACAAGCAGGC CAGCGTGAGG GCCAGCGAGG GGGTGACCTC AGACGGGGCA 
GCTTGCAGCC TGACACCAGG GGAGCACAGC CAGTCTCTAG GTGACTTTAA GCGGGCCTCA 
GCTTCTGGCA TTCTCCATGA TAGTGTGTGC CCCATCTCTG ATAGGCCTGC TCCTGGAAAG 
GTTGAATACT CGGAGAAGGC CTCTCAGGCC AAAGAGCTCC TTCGAAGTGA AAAACTAGAC 
AGCAAGCTGG CCAATATTGA TTACCTCAGA AAGAAAATGT CATTGGATGA CAAAGATGAC 
AGCCACTGTG CCATCCTGAA ACCCAAGATA ACATCTAGCG CCCATGAATG TCTGCCAGGG 
AACCCCATAC GGCCCATGGC AGGGCAACAA GAGACCCCGC CAGCCTCTGA AAACCGGGCA 
TTCATCAACA GTACCCACAC ACCTCAGATG AGTGCAGTTT CCTTTGTTCC TCTCAAAGCC 
TTAGCTGGCC GGGTAGAGAA CGGAGGGGAG AAAGCAGGCT TAGCTGCTCC CGAGTCCCCT 
GTCAGGAAGA GCCCCTCCGA GTATAAGCTA GAGGGCAGGT CAGTTTCATG TCTCAAGCCG 
ATCGAGGGCA CACTGGACAT TGCTCTCCTG TCTGGACCTC ACGCCTCCAA AACAGAGTTG 
CTTTCCCCAG AGCCTGCACA GAGTCCCAGC CCAGGCATCA ACGTGGGACC ATGTGTGCCA 
CTAGCTCTTC CTGGGAGCAG TGGGAAAAAG GGAGACTCCA CCAGCCTGAG AGAGCCTTCC 
TCAGCCAACT TAAAAGTAAA TAAATCTTAT CTGCTGGAGC CTCGGTTCCT ACCCCCGAGC 
CGGGCTCTCC AGGACTCTCT CGCAGCCTCT GGGCCAGAAC CGAAGTCAAA GCCGGAAAGG 
AAGCTCATTC ATCCTTCTGC CAGGAGCCCA GCAACTGTCA CAGAGAGCAA TCTTCAGCAG 
AAAGAGGGTG GTCCCGCCAC ACACCAAGAC CGCTCCACTG ACACCAGGAA CCTCCCTGGC 
CCAGGGCAGA CCCTACACAA TGTGGACCTA CCCAGGCTGT GTACACGTGC CCCACTCCCA 
CCGGAAGGGA CGCCCGCAAA GGAGAAGCCA TGTCTGAAGG AACCCTCTGC CAAGGTGAAA 
AGCGAGTGGT CTGCCGTGAG GGATGACGGA CACAGAGATC CCTGTGCGAA GCTGTGCCCG 
GCAGAGACTG GTAAAGCCAG CGACAGTTCC AAACCCCTGC CTTCCGGGGG GAGGACCCAA 
CCCGATTTCT ACAAGCAGAC CCAGACTTCG GAGAAAGCAT GGGCGCATGC AAAAACAAAC 
CACAAAGATA GCCAAGATGA GGTGAAGTCC CTGGCCAGGG AGGACTCAGC TTCACTTTTA 
TATGAAAAGG AGATAGGCCG GGCACGAAAA GGTCCTGAAC CCAAACCGGA AGTTCCTGCT 
ACCCGGTGCC CTCCTCAGCC ACCAGGAATT GAGGGTGAGA AGCGAGAAAA GCTCTCCGCT 
GCCCCCTCTT TGCAGAAACA GGCTCCCAAA GAGCCAGACA GGAAGGAACA GACTTCGCAA 
AGGCCTGGAG GTAGTGGCCC TCAACAACCC CCACCCACCA AAGAGCTGTC TAACTCAGCA 
TCCTGGCAGC ACGGCAGTTC TCCGAGTCAC ACTTTAAAGA AGGAGCCCGG GACCAAAGCT 
GCCGCTGCAG AACCAAGCAC CAGCCTTCAT GACACTCCCC GATCTGCTAC AGCCACCACC 
ACTGCCATTG CCACCACCAC CACTACCACC AGTGCCGGGC ACAGTGACTG CAGTAGCCAT 
AAGGCCCGGC CTGGCCCTGA CCCCAGCCCT TCAAAGTCTA AGCACCAAGA CAGGTCCCTC 
TCCTCACAGA AGCTGAGTGC TGGCTCTGCA AAAGGCAAAG AGCCTGTCAC TCAACCCCTG 
GGTGGTTCCA TCAGAGAAGG CAAGGGTGGC AGCAAGGGTC CAGTGGACAC ATTTTCTGCT 
GTCCTGACCA CCCAGGGCAA AGCAAGTGAT GTGCTTGTGC AGGGAGAAGG TCGGGTCTCA 
ATCATTGTCC ACACTGAAGA GTGTCCTCTC GATGCCAAAC TGAAAAACAC CAATGGAGGG 
TGTCCCCCAG AGATGCAGGC GAAGCATCCA CCCAGACAAG GACATCTCAG TGAAGCAGCA 
GACCAGAAGC CACTCATTGC TGGTGAGAAG CAAAGCCCGT CTCCAAAGCA TCCCAAACCA 
TCCACTGTGA AAGATTACCC CAGTCTGTGC AGACAGACAG ACAGAAGCCC AAGCCATCAG 
GCTACCACTG GGGACAGGAA GGCAGAAGGA AAGAAATGCA CAGACGCACT TTATGTCGCA 
GCCCCAGAGG GCTACAAGCC AGAGGCCAGC CCTTCTCTCC ACCACGGCGA GACCGGACTC 
AGAGGCTCAG AGAGGCCACC CATGGGCATG GGGAAGGGCT TCTCTGAGCC CAAGGGGAAA 
GGGCCAGGTC CCCAGAAGTC ACTGGCTGAA ACAGGCAAGC CCAGCGGTAT GAAAAGGTCA 
CCCTCTGCCA CCGTGCAGAG CTCTCTCCGC TCAGCTGCCC CCCCAGAAAA GTCTCTGAGT 
TACTCAGCCA GCTTTCCCGA GGCCCAGCCT GGAGTGCGAG AGGTCCCTGC AGCCAACAGC 
AGCCCCTCAT CTGCCAAGGC TACAGGGGGG ACCTCAGAGT TCCCAGCCCC CAGCAGCAGG 
GACCACAGGA AGCTTCAGTC TGGAGGAGAC GGCCGAAGCC AAATGATAAA GAGTGACTCT 
CTGCCCTCCT TCCGCCTCTC CACCTCTGCT CTGGAGTCAC ATTTCCAGGA TCCACAGGTG 
CCCATCGCAT CAGGCCACCG AGGCAGGGCA CTGTCAGTAA CTGCTGCCAC AGGAGAACCC 
AAAGGGAGAG AGCTCGCCCA GCCTCCCCCA GTCAGGAAAC AGAATGCGTG CAGAGAGGCG 
ACCAGAGCAC CCCCAGCCCC AAGCACAGAT CGCTCCCTCC CTCTTTCCTC AGAGAAAGAC 
TTCGTGGTTC GGCAGAGAAG GGGCAAGGAG ACCTTAAGGA GCAGTCCTCA CAAAAAGGCC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_011244673.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242975.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X11, mRNA.

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CloneID	OMu74761
Clone ID Related Accession (Same CDS sequence)	XM_011244673.2 , XM_011244673.3
Accession Version	XM_011244673.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATGT CTGACCCCAA TTTCTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGCACACAA AGTTGCCGAA CAAGCAACCG GAAGAGTTTG 
ATAGGCAATG GCCAGTCTCC AGCACTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGACAG TCCAAGGAAT TTCTCCCCCA GTGCCTCAGC CCATTTCTCA 
TTTGCGAGGA GAACGGATGG GCGCCGCTGG TCTCTGGCTT CTCTCCCTTC CTCAGGCTAT 
GGGACAAATA CCCCAAGCTC CACCGTCTCC TCATCCTGTT CTTCCCAGGA GAAGTTGCAC 
CAGCTACCAT ACCAGCCAAC CCCAGATGAA TTACACTTCT TATCCAAACA CTTCTGCACA 
ACAGAAAGTA TCGCCACTGA GAACCGGTGC AGAAACACAC CCATGCGTCC ACGTTCCCGG 
AGTCTCAGCC CTGGACGGTC CCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCCACA GCTCAGATGG AAGAGCGTCT GAAGGAGATC 
ATCACCAGCT ACTCTCCAGA CCATGTTCTC CCCTTGGCAG ATGGGGTACT TAGTTTCACT 
CACCATCAGA TCATTGAGCT GGCTCGGGAC TGTTTGGATA AATCTCACCA GGGTCTCATC 
ACGTCGAGAT ACTTTTTTGA GTTGCAGCAC AAACTGGACA AGTTGCTCCA GGAGGCTCAC 
GATCGCTCTG AAAGTGGAGA ACTGGCATTT ATCAAGCAAC TAGTCCGAAA GATCCTAATT 
GTCATTGCCC GCCCCGCTCG GTTATTGGAG TGTTTGGAAT TTGATCCTGA AGAATTTTAT 
TACCTATTGG AAGCTGCAGA AGGCCATGCC AAAGAAGGCC AAGGAATCAA AACTGACATC 
CCTAGGTATA TCATCAGCCA GCTGGGACTC AATAAGGACC CTTTAGAAGA AATGGCTCAG 
TTGGGGAATT ACGACAGTAG GACAGCAGAG ACACCAGAGA TGGATGAGTC AGTGAGTAGC 
TCAAATACTT CCCTGAGACT TCGAAGGAAA CCCCGAGAGA GTGATTTTGA AACAATTAAA 
TTGATCAGCA ATGGAGCCTA CGGGGCAGTC TACTTTGTTC GGCACAAAGA GTCCCGCCAG 
AGGTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTCATCC TTCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGAGACAT CCTGACTTTC GCAGAGAACC CCTTTGTGGT CAGCATGTAT 
TGCTCCTTTG AAACGAGGCG TCACTTATGC ATGGTCATGG AGTATGTAGA AGGGGGAGAC 
TGTGCGACCC TAATGAAAAA CATGGGACCT CTCCCAGTTG ATATGGCCAG AATGTATTTC 
GCCGAGACCG TCTTGGCCTT GGAGTACCTG CATAATTACG GAATCGTACA CAGGGACTTG 
AAGCCAGACA ACCTGTTGGT CACCTCCATG GGGCACATAA AACTGACTGA CTTCGGCTTG 
TCTAAGGTGG GATTAATGAG CATGACCACC AATCTCTATG AAGGCCACAT AGAGAAGGAC 
GCTCGAGAGT TCTTAGATAA ACAGGTCTGT GGTACACCTG AGTACATTGC CCCCGAGGTG 
ATTCTGAGAC AGGGCTACGG GAAACCCGTG GACTGGTGGG CCATGGGCAT TATCCTCTAT 
GAATTTCTGG TCGGATGTGT GCCTTTCTTT GGGGACACTC CAGAAGAGCT ATTTGGACAA 
GTCATCAGTG ATGAAATCAA CTGGCCTGAA AAGGACGAGG CCCCTCCTCC AGACGCTCAG 
GAGCTGATTA CCTTGCTCCT CAGGCAGAAT CCGCTGGAGA GGCTGGGAAC AGGTGGAGCC 
TATGAAGTGA AGCAGCATCG TTTCTTCCGC TCCTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCGGAAT TTATTCCCCA ACTGGAATCG GAGGATGACA CAAGTTATTT TGATACTCGG 
TCAGAGAAGT ATCATCACAT GGAGACGGAG GAGGAGGACG ATACAAACGA TGAGGACTTC 
ACCGTGGAGA TAAGGCAGTT TTCTTCCTGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGCA TAACTCAAAA TTCAGGAGAA GACAAAGATG ACTCTGAGGA CAAGACCAAA 
AGCACAACGT TGCCATCCAC AGAGACACTC AGCTGGAGTT CCGAATACTC TGAAATGCAA 
CAGTTATCGA CCTCCAACTC TTCAGATACT GAAAGCAACA GGTGCAAACT CAGCTCTGGC 
TTGCTCCCCA AGCTGGCTAT TTCGACAGAT GGGGAACAAG ATGAGGCTGT CCCTTGCTCT 
GGAGACCCCA GAGAGGAGCC AGAGAAACCT GTCCCTCCCT CTGAGGAGTG TACTCAGGAG 
GAGCCCGAGG TCACCACCCC AGCCAGCACC ATCAGCAGTT CCACACTGTC AGTTGGCAGT 
TTTTCAGAGC ACTTGGATCA GATAAATGGG CGAAGCGAGT GTGTGGACAG TACAGATAAT 
TCCTCAAAGC CATCCAGTGA ACCCACTTCT CACGTGGCTC GACAGCGCTT AGAAAGCACA 
GAGAAAAAGA AAATTTCTGG GAAAGTCACA AAGTCCCTCT CGGCCAGTGC TCTGTCCCTC 
ATGATCCCAG GAGATATGTT CGCTGTATCT CCATTGGGAA GCCCAATGTC CCCACACTCC 
CTGTCTTCAG ACCCTTCTTC TTCACGGGAT TCCTCTCCCA GCCGAGACTC TTCTGCAGCA 
TCTGCCAGTC CGCATCAGCC CATTGTCATC CACAGCTCAG GCAAGAACTA TGGGTTCACC 
ATCCGTGCTA TCCGCGTGTA CGTGGGGGAC AGTGACATCT ACACAGTGCA CCATATCGTC 
TGGAACGTAG AAGAAGGAAG TCCCGCATAC CAGGCAGGAC TGAAGGCCGG GGATCTGATC 
ACACACATCA ACGGAGAGCC GGTGCACGGC CTCGTCCACA CGGAAGTTAT CGAGCTCCTG 
CTGAAGAGTG GGAATAAGGT GTCTATCACC ACTACTCCAT TTGAAAACAC ATCAATCAAA 
ACGGGACCAG CCAGGAGAAA CAGTTACAAG GGCCGGATGG TGAGACGAAG CAAGAAGTCC 
AAGAAGAAGG AGAGTCTAGA AAGGAGGAGA TCTCTCTTCA AGAAGCTGGC CAAGCAGCCT 
TCTCCTTTGC TCCACACCAG CCGAAGTTTC TCCTGCTTAA ACCGGTCCCT GTCATCTGGA 
GAGAGCCTCC CGGGTTCCCC AACTCACAGC TTGTCCCCGA GGTCTCCAAC ACCCAGTTAT 
CGTTCTACTC CCGATTTTCC GTCAGGTACA AATTCCTCCC AGAGCAGCTC CCCAAGTTCA 
AGTGCCCCCA ATTCTCCAGC AGGTTCAGGG CACATCCGGC CCAGCACCCT CCATGGCCTG 
GCTCCCAAAC TCAGCGGGCA GCGATACCGC TCTGGAAGAC GGAAGTCGGC TGGCAGCATC 
CCTCTCTCCC CGCTGGCCAG GACACCCTCT CCCACTCCAC AGCCTACCTC TCCTCAGCGT 
TCACCATCCC CACTGTTGGG ACACTCACTG GGCAATGCCA AGATCACTCA GGCCTTTCCT 
AGCAAGATGC ACTCTCCCCC CACAATCGTC AGACACATCG TGAGGCCCAA GAGTGCAGAG 
CCGCCCCGCT CCCCACTGCT GAAACGGGTG CAGTCGGAGG AAAAGTTGTC ACCCTCCTAT 
GGCAGTGACA AGAAGCTTCT GTGCTCCCGC AAGCATAGCC TAGAGGTGAC ACAAGAGGAG 
GTACAGAGGG AGCAGTGTCA GCGGGAAGTG ACACTGCAGA GCCTGGAAGA GAATGTGTGT 
GACGCTCCTT CCCTCAGTCG GGCCAGGCCA GTGGAGCAAG GCTGTCTGAA ACGCCCCGTG 
TCCCGGAAGG TGGGCAGGCA AGAGTCTGTG GATGACCTGG ACCGGGACAA GCTGAAAGCC 
AAGGTTGTCG TAAAGAAACC AGAAGAGAAA CATGAATCGC ACCAGAAACC TCACAGCCTT 
GGTGGTGATT CGGAAAGCTA TGCTCTCTTC AGGCTAGAGG AGAGAGAGAA AAAAGTGTAC 
TCCAAGGGGT TGGAAAGGTC AGGCCATTTT GAAAACACAT CAGCAGAGTT GCCTTCTGTG 
GGCAGCCTGC TGAAGGACAC TCTTCACAAG CAGGCCAGCG TGAGGGCCAG CGAGGGGGTG 
ACCTCAGACG GGGCAGCTTG CAGCCTGACA CCAGGGGAGC ACAGCCAGTC TCTAGGTGAC 
TTTAAGCGGG CCTCAGCTTC TGGCATTCTC CATGATAGTG TGTGCCCCAT CTCTGATAGG 
CCTGCTCCTG GAAAGGTTGA ATACTCGGAG AAGGCCTCTC AGGCCAAAGA GCTCCTTCGA 
AGTGAAAAAC TAGACAGCAA GCTGGCCAAT ATTGATTACC TCAGAAAGAA AATGTCATTG 
GATGACAAAG ATGACAGCCA CTGTGCCATC CTGAAACCCA AGATAACATC TAGCGCCCAT 
GAATGTCTGC CAGGGAACCC CATACGGCCC ATGGCAGGGC AACAAGAGAC CCCGCCAGCC 
TCTGAAAACC GGGCATTCAT CAACAGTACC CACACACCTC AGATGAGTGC AGTTTCCTTT 
GTTCCTCTCA AAGCCTTAGC TGGCCGGGTA GAGAACGGAG GGGAGAAAGC AGGCTTAGCT 
GCTCCCGAGT CCCCTGTCAG GAAGAGCCCC TCCGAGTATA AGCTAGAGGG CAGGTCAGTT 
TCATGTCTCA AGCCGATCGA GGGCACACTG GACATTGCTC TCCTGTCTGG ACCTCACGCC 
TCCAAAACAG AGTTGCTTTC CCCAGAGCCT GCACAGAGTC CCAGCCCAGG CATCAACGTG 
GGACCATGTG TGCCACTAGC TCTTCCTGGG AGCAGTGGGA AAAAGGGAGA CTCCACCAGC 
CTGAGAGAGC CTTCCTCAGC CAACTTAAAA GTAAATAAAT CTTATCTGCT GGAGCCTCGG 
TTCCTACCCC CGAGCCGGGC TCTCCAGGAC TCTCTCGCAG CCTCTGGGCC AGAACCGAAG 
TCAAAGCCGG AAAGGAAGCT CATTCATCCT TCTGCCAGGA GCCCAGCAAC TGTCACAGAG 
AGCAATCTTC AGCAGAAAGA GGGTGGTCCC GCCACACACC AAGACCGCTC CACTGACACC 
AGGAACCTCC CTGGCCCAGG GCAGACCCTA CACAATGTGG ACCTACCCAG GCTGTGTACA 
CGTGCCCCAC TCCCACCGGA AGGGACGCCC GCAAAGGAGA AGCCATGTCT GAAGGAACCC 
TCTGCCAAGG TGAAAAGCGA GTGGTCTGCC GTGAGGGATG ACGGACACAG AGATCCCTGT 
GCGAAGCTGT GCCCGGCAGA GACTGGTAAA GCCAGCGACA GTTCCAAACC CCTGCCTTCC 
GGGGGGAGGA CCCAACCCGA TTTCTACAAG CAGACCCAGA CTTCGGAGAA AGCATGGGCG 
CATGCAAAAA CAAACCACAA AGATAGCCAA GATGAGGTGA AGTCCCTGGC CAGGGAGGAC 
TCAGCTTCAC TTTTATATGA AAAGGAGATA GGCCGGGCAC GAAAAGGTCC TGAACCCAAA 
CCGGAAGTTC CTGCTACCCG GTGCCCTCCT CAGCCACCAG GAATTGAGGG TGAGAAGCGA 
GAAAAGCTCT CCGCTGCCCC CTCTTTGCAG AAACAGGCTC CCAAAGAGCC AGACAGGAAG 
GAACAGACTT CGCAAAGGCC TGGAGGTAGT GGCCCTCAAC AACCCCCACC CACCAAAGAG 
CTGTCTAACT CAGCATCCTG GCAGCACGGC AGTTCTCCGA GTCACACTTT AAAGAAGGAG 
CCCGGGACCA AAGCTGCCGC TGCAGAACCA AGCACCAGCC TTCATGACAC TCCCCGATCT 
GCTACAGCCA CCACCACTGC CATTGCCACC ACCACCACTA CCACCAGTGC CGGGCACAGT 
GACTGCAGTA GCCATAAGGC CCGGCCTGGC CCTGACCCCA GCCCTTCAAA GTCTAAGCAC 
CAAGACAGGT CCCTCTCCTC ACAGAAGCTG AGTGCTGGCT CTGCAAAAGG CAAAGAGCCT 
GTCACTCAAC CCCTGGGTGG TTCCATCAGA GAAGGCAAGG GTGGCAGCAA GGGTCCAGTG 
GACACATTTT CTGCTGTCCT GACCACCCAG GGCAAAGCAA GTGATGTGCT TGTGCAGGGA 
GAAGGTCGGG TCTCAATCAT TGTCCACACT GAAGAGTGTC CTCTCGATGC CAAACTGAAA 
AACACCAATG GAGGGTGTCC CCCAGAGATG CAGGCGAAGC ATCCACCCAG ACAAGGACAT 
CTCAGTGAAG CAGCAGACCA GAAGCCACTC ATTGCTGGTG AGAAGCAAAG CCCGTCTCCA 
AAGCATCCCA AACCATCCAC TGTGAAAGAT TACCCCAGTC TGTGCAGACA GACAGACAGA 
AGCCCAAGCC ATCAGGCTAC CACTGGGGAC AGGAAGGCAG AAGGAAAGAA ATGCACAGAC 
GCACTTTATG TCGCAGCCCC AGAGGGCTAC AAGCCAGAGG CCAGCCCTTC TCTCCACCAC 
GGCGAGACCG GACTCAGAGG CTCAGAGAGG CCACCCATGG GCATGGGGAA GGGCTTCTCT 
GAGCCCAAGG GGAAAGGGCC AGGTCCCCAG AAGTCACTGG CTGAAACAGG CAAGCCCAGC 
GGTATGAAAA GGTCACCCTC TGCCACCGTG CAGAGCTCTC TCCGCTCAGC TGCCCCCCCA 
GAAAAGTCTC TGAGTTACTC AGCCAGCTTT CCCGAGGCCC AGCCTGGAGT GCGAGAGGTC 
CCTGCAGCCA ACAGCAGCCC CTCATCTGCC AAGGCTACAG GGGGGACCTC AGAGTTCCCA 
GCCCCCAGCA GCAGGGACCA CAGGAAGCTT CAGTCTGGAG GAGACGGCCG AAGCCAAATG 
ATAAAGAGTG ACTCTCTGCC CTCCTTCCGC CTCTCCACCT CTGCTCTGGA GTCACATTTC 
CAGGATCCAC AGGTGCCCAT CGCATCAGGC CACCGAGGCA GGGCACTGTC AGTAACTGCT 
GCCACAGGAG AACCCAAAGG GAGAGAGCTC GCCCAGCCTC CCCCAGTCAG GAAACAGAAT 
GCGTGCAGAG AGGCGACCAG AGCACCCCCA GCCCCAAGCA CAGATCGCTC CCTCCCTCTT 
TCCTCAGAGA AAGACTTCGT GGTTCGGCAG AGAAGGGGCA AGGAGACCTT AAGGAGCAGT 
CCTCACAAAA AGGCCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242975.1
CDS	1..7281
Translation	MDMSDPNFWTVLSNFTLPHLRSGNRLRRTQSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_011244673.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244673.2

ORF Insert Sequence:

ATGGATATGT CTGACCCCAA TTTCTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGCACACAA AGTTGCCGAA CAAGCAACCG GAAGAGTTTG 
ATAGGCAATG GCCAGTCTCC AGCACTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGACAG TCCAAGGAAT TTCTCCCCCA GTGCCTCAGC CCATTTCTCA 
TTTGCGAGGA GAACGGATGG GCGCCGCTGG TCTCTGGCTT CTCTCCCTTC CTCAGGCTAT 
GGGACAAATA CCCCAAGCTC CACCGTCTCC TCATCCTGTT CTTCCCAGGA GAAGTTGCAC 
CAGCTACCAT ACCAGCCAAC CCCAGATGAA TTACACTTCT TATCCAAACA CTTCTGCACA 
ACAGAAAGTA TCGCCACTGA GAACCGGTGC AGAAACACAC CCATGCGTCC ACGTTCCCGG 
AGTCTCAGCC CTGGACGGTC CCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCCACA GCTCAGATGG AAGAGCGTCT GAAGGAGATC 
ATCACCAGCT ACTCTCCAGA CCATGTTCTC CCCTTGGCAG ATGGGGTACT TAGTTTCACT 
CACCATCAGA TCATTGAGCT GGCTCGGGAC TGTTTGGATA AATCTCACCA GGGTCTCATC 
ACGTCGAGAT ACTTTTTTGA GTTGCAGCAC AAACTGGACA AGTTGCTCCA GGAGGCTCAC 
GATCGCTCTG AAAGTGGAGA ACTGGCATTT ATCAAGCAAC TAGTCCGAAA GATCCTAATT 
GTCATTGCCC GCCCCGCTCG GTTATTGGAG TGTTTGGAAT TTGATCCTGA AGAATTTTAT 
TACCTATTGG AAGCTGCAGA AGGCCATGCC AAAGAAGGCC AAGGAATCAA AACTGACATC 
CCTAGGTATA TCATCAGCCA GCTGGGACTC AATAAGGACC CTTTAGAAGA AATGGCTCAG 
TTGGGGAATT ACGACAGTAG GACAGCAGAG ACACCAGAGA TGGATGAGTC AGTGAGTAGC 
TCAAATACTT CCCTGAGACT TCGAAGGAAA CCCCGAGAGA GTGATTTTGA AACAATTAAA 
TTGATCAGCA ATGGAGCCTA CGGGGCAGTC TACTTTGTTC GGCACAAAGA GTCCCGCCAG 
AGGTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTCATCC TTCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGAGACAT CCTGACTTTC GCAGAGAACC CCTTTGTGGT CAGCATGTAT 
TGCTCCTTTG AAACGAGGCG TCACTTATGC ATGGTCATGG AGTATGTAGA AGGGGGAGAC 
TGTGCGACCC TAATGAAAAA CATGGGACCT CTCCCAGTTG ATATGGCCAG AATGTATTTC 
GCCGAGACCG TCTTGGCCTT GGAGTACCTG CATAATTACG GAATCGTACA CAGGGACTTG 
AAGCCAGACA ACCTGTTGGT CACCTCCATG GGGCACATAA AACTGACTGA CTTCGGCTTG 
TCTAAGGTGG GATTAATGAG CATGACCACC AATCTCTATG AAGGCCACAT AGAGAAGGAC 
GCTCGAGAGT TCTTAGATAA ACAGGTCTGT GGTACACCTG AGTACATTGC CCCCGAGGTG 
ATTCTGAGAC AGGGCTACGG GAAACCCGTG GACTGGTGGG CCATGGGCAT TATCCTCTAT 
GAATTTCTGG TCGGATGTGT GCCTTTCTTT GGGGACACTC CAGAAGAGCT ATTTGGACAA 
GTCATCAGTG ATGAAATCAA CTGGCCTGAA AAGGACGAGG CCCCTCCTCC AGACGCTCAG 
GAGCTGATTA CCTTGCTCCT CAGGCAGAAT CCGCTGGAGA GGCTGGGAAC AGGTGGAGCC 
TATGAAGTGA AGCAGCATCG TTTCTTCCGC TCCTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCGGAAT TTATTCCCCA ACTGGAATCG GAGGATGACA CAAGTTATTT TGATACTCGG 
TCAGAGAAGT ATCATCACAT GGAGACGGAG GAGGAGGACG ATACAAACGA TGAGGACTTC 
ACCGTGGAGA TAAGGCAGTT TTCTTCCTGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGCA TAACTCAAAA TTCAGGAGAA GACAAAGATG ACTCTGAGGA CAAGACCAAA 
AGCACAACGT TGCCATCCAC AGAGACACTC AGCTGGAGTT CCGAATACTC TGAAATGCAA 
CAGTTATCGA CCTCCAACTC TTCAGATACT GAAAGCAACA GGTGCAAACT CAGCTCTGGC 
TTGCTCCCCA AGCTGGCTAT TTCGACAGAT GGGGAACAAG ATGAGGCTGT CCCTTGCTCT 
GGAGACCCCA GAGAGGAGCC AGAGAAACCT GTCCCTCCCT CTGAGGAGTG TACTCAGGAG 
GAGCCCGAGG TCACCACCCC AGCCAGCACC ATCAGCAGTT CCACACTGTC AGTTGGCAGT 
TTTTCAGAGC ACTTGGATCA GATAAATGGG CGAAGCGAGT GTGTGGACAG TACAGATAAT 
TCCTCAAAGC CATCCAGTGA ACCCACTTCT CACGTGGCTC GACAGCGCTT AGAAAGCACA 
GAGAAAAAGA AAATTTCTGG GAAAGTCACA AAGTCCCTCT CGGCCAGTGC TCTGTCCCTC 
ATGATCCCAG GAGATATGTT CGCTGTATCT CCATTGGGAA GCCCAATGTC CCCACACTCC 
CTGTCTTCAG ACCCTTCTTC TTCACGGGAT TCCTCTCCCA GCCGAGACTC TTCTGCAGCA 
TCTGCCAGTC CGCATCAGCC CATTGTCATC CACAGCTCAG GCAAGAACTA TGGGTTCACC 
ATCCGTGCTA TCCGCGTGTA CGTGGGGGAC AGTGACATCT ACACAGTGCA CCATATCGTC 
TGGAACGTAG AAGAAGGAAG TCCCGCATAC CAGGCAGGAC TGAAGGCCGG GGATCTGATC 
ACACACATCA ACGGAGAGCC GGTGCACGGC CTCGTCCACA CGGAAGTTAT CGAGCTCCTG 
CTGAAGAGTG GGAATAAGGT GTCTATCACC ACTACTCCAT TTGAAAACAC ATCAATCAAA 
ACGGGACCAG CCAGGAGAAA CAGTTACAAG GGCCGGATGG TGAGACGAAG CAAGAAGTCC 
AAGAAGAAGG AGAGTCTAGA AAGGAGGAGA TCTCTCTTCA AGAAGCTGGC CAAGCAGCCT 
TCTCCTTTGC TCCACACCAG CCGAAGTTTC TCCTGCTTAA ACCGGTCCCT GTCATCTGGA 
GAGAGCCTCC CGGGTTCCCC AACTCACAGC TTGTCCCCGA GGTCTCCAAC ACCCAGTTAT 
CGTTCTACTC CCGATTTTCC GTCAGGTACA AATTCCTCCC AGAGCAGCTC CCCAAGTTCA 
AGTGCCCCCA ATTCTCCAGC AGGTTCAGGG CACATCCGGC CCAGCACCCT CCATGGCCTG 
GCTCCCAAAC TCAGCGGGCA GCGATACCGC TCTGGAAGAC GGAAGTCGGC TGGCAGCATC 
CCTCTCTCCC CGCTGGCCAG GACACCCTCT CCCACTCCAC AGCCTACCTC TCCTCAGCGT 
TCACCATCCC CACTGTTGGG ACACTCACTG GGCAATGCCA AGATCACTCA GGCCTTTCCT 
AGCAAGATGC ACTCTCCCCC CACAATCGTC AGACACATCG TGAGGCCCAA GAGTGCAGAG 
CCGCCCCGCT CCCCACTGCT GAAACGGGTG CAGTCGGAGG AAAAGTTGTC ACCCTCCTAT 
GGCAGTGACA AGAAGCTTCT GTGCTCCCGC AAGCATAGCC TAGAGGTGAC ACAAGAGGAG 
GTACAGAGGG AGCAGTGTCA GCGGGAAGTG ACACTGCAGA GCCTGGAAGA GAATGTGTGT 
GACGCTCCTT CCCTCAGTCG GGCCAGGCCA GTGGAGCAAG GCTGTCTGAA ACGCCCCGTG 
TCCCGGAAGG TGGGCAGGCA AGAGTCTGTG GATGACCTGG ACCGGGACAA GCTGAAAGCC 
AAGGTTGTCG TAAAGAAACC AGAAGAGAAA CATGAATCGC ACCAGAAACC TCACAGCCTT 
GGTGGTGATT CGGAAAGCTA TGCTCTCTTC AGGCTAGAGG AGAGAGAGAA AAAAGTGTAC 
TCCAAGGGGT TGGAAAGGTC AGGCCATTTT GAAAACACAT CAGCAGAGTT GCCTTCTGTG 
GGCAGCCTGC TGAAGGACAC TCTTCACAAG CAGGCCAGCG TGAGGGCCAG CGAGGGGGTG 
ACCTCAGACG GGGCAGCTTG CAGCCTGACA CCAGGGGAGC ACAGCCAGTC TCTAGGTGAC 
TTTAAGCGGG CCTCAGCTTC TGGCATTCTC CATGATAGTG TGTGCCCCAT CTCTGATAGG 
CCTGCTCCTG GAAAGGTTGA ATACTCGGAG AAGGCCTCTC AGGCCAAAGA GCTCCTTCGA 
AGTGAAAAAC TAGACAGCAA GCTGGCCAAT ATTGATTACC TCAGAAAGAA AATGTCATTG 
GATGACAAAG ATGACAGCCA CTGTGCCATC CTGAAACCCA AGATAACATC TAGCGCCCAT 
GAATGTCTGC CAGGGAACCC CATACGGCCC ATGGCAGGGC AACAAGAGAC CCCGCCAGCC 
TCTGAAAACC GGGCATTCAT CAACAGTACC CACACACCTC AGATGAGTGC AGTTTCCTTT 
GTTCCTCTCA AAGCCTTAGC TGGCCGGGTA GAGAACGGAG GGGAGAAAGC AGGCTTAGCT 
GCTCCCGAGT CCCCTGTCAG GAAGAGCCCC TCCGAGTATA AGCTAGAGGG CAGGTCAGTT 
TCATGTCTCA AGCCGATCGA GGGCACACTG GACATTGCTC TCCTGTCTGG ACCTCACGCC 
TCCAAAACAG AGTTGCTTTC CCCAGAGCCT GCACAGAGTC CCAGCCCAGG CATCAACGTG 
GGACCATGTG TGCCACTAGC TCTTCCTGGG AGCAGTGGGA AAAAGGGAGA CTCCACCAGC 
CTGAGAGAGC CTTCCTCAGC CAACTTAAAA GTAAATAAAT CTTATCTGCT GGAGCCTCGG 
TTCCTACCCC CGAGCCGGGC TCTCCAGGAC TCTCTCGCAG CCTCTGGGCC AGAACCGAAG 
TCAAAGCCGG AAAGGAAGCT CATTCATCCT TCTGCCAGGA GCCCAGCAAC TGTCACAGAG 
AGCAATCTTC AGCAGAAAGA GGGTGGTCCC GCCACACACC AAGACCGCTC CACTGACACC 
AGGAACCTCC CTGGCCCAGG GCAGACCCTA CACAATGTGG ACCTACCCAG GCTGTGTACA 
CGTGCCCCAC TCCCACCGGA AGGGACGCCC GCAAAGGAGA AGCCATGTCT GAAGGAACCC 
TCTGCCAAGG TGAAAAGCGA GTGGTCTGCC GTGAGGGATG ACGGACACAG AGATCCCTGT 
GCGAAGCTGT GCCCGGCAGA GACTGGTAAA GCCAGCGACA GTTCCAAACC CCTGCCTTCC 
GGGGGGAGGA CCCAACCCGA TTTCTACAAG CAGACCCAGA CTTCGGAGAA AGCATGGGCG 
CATGCAAAAA CAAACCACAA AGATAGCCAA GATGAGGTGA AGTCCCTGGC CAGGGAGGAC 
TCAGCTTCAC TTTTATATGA AAAGGAGATA GGCCGGGCAC GAAAAGGTCC TGAACCCAAA 
CCGGAAGTTC CTGCTACCCG GTGCCCTCCT CAGCCACCAG GAATTGAGGG TGAGAAGCGA 
GAAAAGCTCT CCGCTGCCCC CTCTTTGCAG AAACAGGCTC CCAAAGAGCC AGACAGGAAG 
GAACAGACTT CGCAAAGGCC TGGAGGTAGT GGCCCTCAAC AACCCCCACC CACCAAAGAG 
CTGTCTAACT CAGCATCCTG GCAGCACGGC AGTTCTCCGA GTCACACTTT AAAGAAGGAG 
CCCGGGACCA AAGCTGCCGC TGCAGAACCA AGCACCAGCC TTCATGACAC TCCCCGATCT 
GCTACAGCCA CCACCACTGC CATTGCCACC ACCACCACTA CCACCAGTGC CGGGCACAGT 
GACTGCAGTA GCCATAAGGC CCGGCCTGGC CCTGACCCCA GCCCTTCAAA GTCTAAGCAC 
CAAGACAGGT CCCTCTCCTC ACAGAAGCTG AGTGCTGGCT CTGCAAAAGG CAAAGAGCCT 
GTCACTCAAC CCCTGGGTGG TTCCATCAGA GAAGGCAAGG GTGGCAGCAA GGGTCCAGTG 
GACACATTTT CTGCTGTCCT GACCACCCAG GGCAAAGCAA GTGATGTGCT TGTGCAGGGA 
GAAGGTCGGG TCTCAATCAT TGTCCACACT GAAGAGTGTC CTCTCGATGC CAAACTGAAA 
AACACCAATG GAGGGTGTCC CCCAGAGATG CAGGCGAAGC ATCCACCCAG ACAAGGACAT 
CTCAGTGAAG CAGCAGACCA GAAGCCACTC ATTGCTGGTG AGAAGCAAAG CCCGTCTCCA 
AAGCATCCCA AACCATCCAC TGTGAAAGAT TACCCCAGTC TGTGCAGACA GACAGACAGA 
AGCCCAAGCC ATCAGGCTAC CACTGGGGAC AGGAAGGCAG AAGGAAAGAA ATGCACAGAC 
GCACTTTATG TCGCAGCCCC AGAGGGCTAC AAGCCAGAGG CCAGCCCTTC TCTCCACCAC 
GGCGAGACCG GACTCAGAGG CTCAGAGAGG CCACCCATGG GCATGGGGAA GGGCTTCTCT 
GAGCCCAAGG GGAAAGGGCC AGGTCCCCAG AAGTCACTGG CTGAAACAGG CAAGCCCAGC 
GGTATGAAAA GGTCACCCTC TGCCACCGTG CAGAGCTCTC TCCGCTCAGC TGCCCCCCCA 
GAAAAGTCTC TGAGTTACTC AGCCAGCTTT CCCGAGGCCC AGCCTGGAGT GCGAGAGGTC 
CCTGCAGCCA ACAGCAGCCC CTCATCTGCC AAGGCTACAG GGGGGACCTC AGAGTTCCCA 
GCCCCCAGCA GCAGGGACCA CAGGAAGCTT CAGTCTGGAG GAGACGGCCG AAGCCAAATG 
ATAAAGAGTG ACTCTCTGCC CTCCTTCCGC CTCTCCACCT CTGCTCTGGA GTCACATTTC 
CAGGATCCAC AGGTGCCCAT CGCATCAGGC CACCGAGGCA GGGCACTGTC AGTAACTGCT 
GCCACAGGAG AACCCAAAGG GAGAGAGCTC GCCCAGCCTC CCCCAGTCAG GAAACAGAAT 
GCGTGCAGAG AGGCGACCAG AGCACCCCCA GCCCCAAGCA CAGATCGCTC CCTCCCTCTT 
TCCTCAGAGA AAGACTTCGT GGTTCGGCAG AGAAGGGGCA AGGAGACCTT AAGGAGCAGT 
CCTCACAAAA AGGCCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_011244674.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242976.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X16, mRNA.

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CloneID	OMu74762
Clone ID Related Accession (Same CDS sequence)	XM_011244674.3 , XM_011244674.2
Accession Version	XM_011244674.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATGT CTGACCCCAA TTTCTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGCACACAA AGTTGCCGAA CAAGCAACCG GAAGAGTTTG 
ATAGGCAATG GCCAGTCTCC AGCACTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGACAG TCCAAGGAAT TTCTCCCCCA GTGCCTCAGC CCATTTCTCA 
TTTGCGAGGA GAACGGATGG GCGCCGCTGG TCTCTGGCTT CTCTCCCTTC CTCAGGCTAT 
GGGACAAATA CCCCAAGCTC CACCGTCTCC TCATCCTGTT CTTCCCAGGA GAAGTTGCAC 
CAGCTACCAT ACCAGCCAAC CCCAGATGAA TTACACTTCT TATCCAAACA CTTCTGCACA 
ACAGAAAGTA TCGCCACTGA GAACCGGTGC AGAAACACAC CCATGCGTCC ACGTTCCCGG 
AGTCTCAGCC CTGGACGGTC CCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCCACA GCTCAGATGG AAGAGCGTCT GAAGGAGATC 
ATCACCAGCT ACTCTCCAGA CCATGTTCTC CCCTTGGCAG ATGGGGTACT TAGTTTCACT 
CACCATCAGA TCATTGAGCT GGCTCGGGAC TGTTTGGATA AATCTCACCA GGGTCTCATC 
ACGTCGAGAT ACTTTTTTGA GTTGCAGCAC AAACTGGACA AGTTGCTCCA GGAGGCTCAC 
GATCGCTCTG AAAGTGGAGA ACTGGCATTT ATCAAGCAAC TAGTCCGAAA GATCCTAATT 
GTCATTGCCC GCCCCGCTCG GTTATTGGAG TGTTTGGAAT TTGATCCTGA AGAATTTTAT 
TACCTATTGG AAGCTGCAGA AGGCCATGCC AAAGAAGGCC AAGGAATCAA AACTGACATC 
CCTAGGTATA TCATCAGCCA GCTGGGACTC AATAAGGACC CTTTAGAAGA AATGGCTCAG 
TTGGGGAATT ACGACAGTAG GACAGCAGAG ACACCAGAGA TGGATGAGTC AGTGAGTAGC 
TCAAATACTT CCCTGAGACT TCGAAGGAAA CCCCGAGAGA GTGATTTTGA AACAATTAAA 
TTGATCAGCA ATGGAGCCTA CGGGGCAGTC TACTTTGTTC GGCACAAAGA GTCCCGCCAG 
AGGTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTCATCC TTCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGAGACAT CCTGACTTTC GCAGAGAACC CCTTTGTGGT CAGCATGTAT 
TGCTCCTTTG AAACGAGGCG TCACTTATGC ATGGTCATGG AGTATGTAGA AGGGGGAGAC 
TGTGCGACCC TAATGAAAAA CATGGGACCT CTCCCAGTTG ATATGGCCAG AATGTATTTC 
GCCGAGACCG TCTTGGCCTT GGAGTACCTG CATAATTACG GAATCGTACA CAGGGACTTG 
AAGCCAGACA ACCTGTTGGT CACCTCCATG GGGCACATAA AACTGACTGA CTTCGGCTTG 
TCTAAGGTGG GATTAATGAG CATGACCACC AATCTCTATG AAGGCCACAT AGAGAAGGAC 
GCTCGAGAGT TCTTAGATAA ACAGGTCTGT GGTACACCTG AGTACATTGC CCCCGAGGTG 
ATTCTGAGAC AGGGCTACGG GAAACCCGTG GACTGGTGGG CCATGGGCAT TATCCTCTAT 
GAATTTCTGG TCGGATGTGT GCCTTTCTTT GGGGACACTC CAGAAGAGCT ATTTGGACAA 
GTCATCAGTG ATGAAATCAA CTGGCCTGAA AAGGACGAGG CCCCTCCTCC AGACGCTCAG 
GAGCTGATTA CCTTGCTCCT CAGGCAGAAT CCGCTGGAGA GGCTGGGAAC AGGTGGAGCC 
TATGAAGTGA AGCAGCATCG TTTCTTCCGC TCCTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCGGAAT TTATTCCCCA ACTGGAATCG GAGGATGACA CAAGTTATTT TGATACTCGG 
TCAGAGAAGT ATCATCACAT GGAGACGGAG GAGGAGGACG ATACAAACGA TGAGGACTTC 
ACCGTGGAGA TAAGGCAGTT TTCTTCCTGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGCA TAACTCAAAA TTCAGGAGAA GACAAAGATG ACTCTGAGGA CAAGACCAAA 
AGCACAACGT TGCCATCCAC AGAGACACTC AGCTGGAGTT CCGAATACTC TGAAATGCAA 
CAGTTATCGA CCTCCAACTC TTCAGATACT GAAAGCAACA GGTGCAAACT CAGCTCTGGC 
TTGCTCCCCA AGCTGGCTAT TTCGACAGAT GGGGAACAAG ATGAGGCTGT CCCTTGCTCT 
GGAGACCCCA GAGAGGAGCC AGAGAAACCT GTCCCTCCCT CTGAGGAGTG TACTCAGGAG 
GAGCCCGAGG TCACCACCCC AGCCAGCACC ATCAGCAGTT CCACACTGTC AGATATGTTC 
GCTGTATCTC CATTGGGAAG CCCAATGTCC CCACACTCCC TGTCTTCAGA CCCTTCTTCT 
TCACGGGATT CCTCTCCCAG CCGAGACTCT TCTGCAGCAT CTGCCAGTCC GCATCAGCCC 
ATTGTCATCC ACAGCTCAGG CAAGAACTAT GGGTTCACCA TCCGTGCTAT CCGCGTGTAC 
GTGGGGGACA GTGACATCTA CACAGTGCAC CATATCGTCT GGAACGTAGA AGAAGGAAGT 
CCCGCATACC AGGCAGGACT GAAGGCCGGG GATCTGATCA CACACATCAA CGGAGAGCCG 
GTGCACGGCC TCGTCCACAC GGAAGTTATC GAGCTCCTGC TGAAGAGTGG GAATAAGGTG 
TCTATCACCA CTACTCCATT TGAAAACACA TCAATCAAAA CGGGACCAGC CAGGAGAAAC 
AGTTACAAGG GCCGGATGGT GAGACGAAGC AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA 
AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC AAGCAGCCTT CTCCTTTGCT CCACACCAGC 
CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG TCATCTGGAG AGAGCCTCCC GGGTTCCCCA 
ACTCACAGCT TGTCCCCGAG GTCTCCAACA CCCAGTTATC GTTCTACTCC CGATTTTCCG 
TCAGGTACAA ATTCCTCCCA GAGCAGCTCC CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA 
GGTTCAGGGC ACATCCGGCC CAGCACCCTC CATGGCCTGG CTCCCAAACT CAGCGGGCAG 
CGATACCGCT CTGGAAGACG GAAGTCGGCT GGCAGCATCC CTCTCTCCCC GCTGGCCAGG 
ACACCCTCTC CCACTCCACA GCCTACCTCT CCTCAGCGTT CACCATCCCC ACTGTTGGGA 
CACTCACTGG GCAATGCCAA GATCACTCAG GCCTTTCCTA GCAAGATGCA CTCTCCCCCC 
ACAATCGTCA GACACATCGT GAGGCCCAAG AGTGCAGAGC CGCCCCGCTC CCCACTGCTG 
AAACGGGTGC AGTCGGAGGA AAAGTTGTCA CCCTCCTATG GCAGTGACAA GAAGCTTCTG 
TGCTCCCGCA AGCATAGCCT AGAGGTGACA CAAGAGGAGG TACAGAGGGA GCAGTGTCAG 
CGGGAAGTGA CACTGCAGAG CCTGGAAGAG AATGTGTGTG ACGCTCCTTC CCTCAGTCGG 
GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA 
GAGTCTGTGG ATGACCTGGA CCGGGACAAG CTGAAAGCCA AGGTTGTCGT AAAGAAACCA 
GAAGAGAAAC ATGAATCGCA CCAGAAACCT CACAGCCTTG GTGGTGATTC GGAAAGCTAT 
GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA AAAGTGTACT CCAAGGGGTT GGAAAGGTCA 
GGCCATTTTG AAAACACATC AGCAGAGTTG CCTTCTGTGG GCAGCCTGCT GAAGGACACT 
CTTCACAAGC AGGCCAGCGT GAGGGCCAGC GAGGGGGTGA CCTCAGACGG GGCAGCTTGC 
AGCCTGACAC CAGGGGAGCA CAGCCAGTCT CTAGGTGACT TTAAGCGGGC CTCAGCTTCT 
GGCATTCTCC ATGATAGTGT GTGCCCCATC TCTGATAGGC CTGCTCCTGG AAAGGTTGAA 
TACTCGGAGA AGGCCTCTCA GGCCAAAGAG CTCCTTCGAA GTGAAAAACT AGACAGCAAG 
CTGGCCAATA TTGATTACCT CAGAAAGAAA ATGTCATTGG ATGACAAAGA TGACAGCCAC 
TGTGCCATCC TGAAACCCAA GATAACATCT AGCGCCCATG AATGTCTGCC AGGGAACCCC 
ATACGGCCCA TGGCAGGGCA ACAAGAGACC CCGCCAGCCT CTGAAAACCG GGCATTCATC 
AACAGTACCC ACACACCTCA GATGAGTGCA GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT 
GGCCGGGTAG AGAACGGAGG GGAGAAAGCA GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG 
AAGAGCCCCT CCGAGTATAA GCTAGAGGGC AGGTCAGTTT CATGTCTCAA GCCGATCGAG 
GGCACACTGG ACATTGCTCT CCTGTCTGGA CCTCACGCCT CCAAAACAGA GTTGCTTTCC 
CCAGAGCCTG CACAGAGTCC CAGCCCAGGC ATCAACGTGG GACCATGTGT GCCACTAGCT 
CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC TCCACCAGCC TGAGAGAGCC TTCCTCAGCC 
AACTTAAAAG TAAATAAATC TTATCTGCTG GAGCCTCGGT TCCTACCCCC GAGCCGGGCT 
CTCCAGGACT CTCTCGCAGC CTCTGGGCCA GAACCGAAGT CAAAGCCGGA AAGGAAGCTC 
ATTCATCCTT CTGCCAGGAG CCCAGCAACT GTCACAGAGA GCAATCTTCA GCAGAAAGAG 
GGTGGTCCCG CCACACACCA AGACCGCTCC ACTGACACCA GGAACCTCCC TGGCCCAGGG 
CAGACCCTAC ACAATGTGGA CCTACCCAGG CTGTGTACAC GTGCCCCACT CCCACCGGAA 
GGGACGCCCG CAAAGGAGAA GCCATGTCTG AAGGAACCCT CTGCCAAGGT GAAAAGCGAG 
TGGTCTGCCG TGAGGGATGA CGGACACAGA GATCCCTGTG CGAAGCTGTG CCCGGCAGAG 
ACTGGTAAAG CCAGCGACAG TTCCAAACCC CTGCCTTCCG GGGGGAGGAC CCAACCCGAT 
TTCTACAAGC AGACCCAGAC TTCGGAGAAA GCATGGGCGC ATGCAAAAAC AAACCACAAA 
GATAGCCAAG ATGAGGTGAA GTCCCTGGCC AGGGAGGACT CAGCTTCACT TTTATATGAA 
AAGGAGATAG GCCGGGCACG AAAAGGTCCT GAACCCAAAC CGGAAGTTCC TGCTACCCGG 
TGCCCTCCTC AGCCACCAGG AATTGAGGGT GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC 
TCTTTGCAGA AACAGGCTCC CAAAGAGCCA GACAGGAAGG AACAGACTTC GCAAAGGCCT 
GGAGGTAGTG GCCCTCAACA ACCCCCACCC ACCAAAGAGC TGTCTAACTC AGCATCCTGG 
CAGCACGGCA GTTCTCCGAG TCACACTTTA AAGAAGGAGC CCGGGACCAA AGCTGCCGCT 
GCAGAACCAA GCACCAGCCT TCATGACACT CCCCGATCTG CTACAGCCAC CACCACTGCC 
ATTGCCACCA CCACCACTAC CACCAGTGCC GGGCACAGTG ACTGCAGTAG CCATAAGGCC 
CGGCCTGGCC CTGACCCCAG CCCTTCAAAG TCTAAGCACC AAGACAGGTC CCTCTCCTCA 
CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC AAAGAGCCTG TCACTCAACC CCTGGGTGGT 
TCCATCAGAG AAGGCAAGGG TGGCAGCAAG GGTCCAGTGG ACACATTTTC TGCTGTCCTG 
ACCACCCAGG GCAAAGCAAG TGATGTGCTT GTGCAGGGAG AAGGTCGGGT CTCAATCATT 
GTCCACACTG AAGAGTGTCC TCTCGATGCC AAACTGAAAA ACACCAATGG AGGGTGTCCC 
CCAGAGATGC AGGCGAAGCA TCCACCCAGA CAAGGACATC TCAGTGAAGC AGCAGACCAG 
AAGCCACTCA TTGCTGGTGA GAAGCAAAGC CCGTCTCCAA AGCATCCCAA ACCATCCACT 
GTGAAAGATT ACCCCAGTCT GTGCAGACAG ACAGACAGAA GCCCAAGCCA TCAGGCTACC 
ACTGGGGACA GGAAGGCAGA AGGAAAGAAA TGCACAGACG CACTTTATGT CGCAGCCCCA 
GAGGGCTACA AGCCAGAGGC CAGCCCTTCT CTCCACCACG GCGAGACCGG ACTCAGAGGC 
TCAGAGAGGC CACCCATGGG CATGGGGAAG GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA 
GGTCCCCAGA AGTCACTGGC TGAAACAGGC AAGCCCAGCG GTATGAAAAG GTCACCCTCT 
GCCACCGTGC AGAGCTCTCT CCGCTCAGCT GCCCCCCCAG AAAAGTCTCT GAGTTACTCA 
GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC 
TCATCTGCCA AGGCTACAGG GGGGACCTCA GAGTTCCCAG CCCCCAGCAG CAGGGACCAC 
AGGAAGCTTC AGTCTGGAGG AGACGGCCGA AGCCAAATGA TAAAGAGTGA CTCTCTGCCC 
TCCTTCCGCC TCTCCACCTC TGCTCTGGAG TCACATTTCC AGGATCCACA GGTGCCCATC 
GCATCAGGCC ACCGAGGCAG GGCACTGTCA GTAACTGCTG CCACAGGAGA ACCCAAAGGG 
AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG AAACAGAATG CGTGCAGAGA GGCGACCAGA 
GCACCCCCAG CCCCAAGCAC AGATCGCTCC CTCCCTCTTT CCTCAGAGAA AGACTTCGTG 
GTTCGGCAGA GAAGGGGCAA GGAGACCTTA AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242976.1
CDS	1..7080
Translation	MDMSDPNFWTVLSNFTLPHLRSGNRLRRTQSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_011244674.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244674.2

ORF Insert Sequence:

ATGGATATGT CTGACCCCAA TTTCTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGCACACAA AGTTGCCGAA CAAGCAACCG GAAGAGTTTG 
ATAGGCAATG GCCAGTCTCC AGCACTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGACAG TCCAAGGAAT TTCTCCCCCA GTGCCTCAGC CCATTTCTCA 
TTTGCGAGGA GAACGGATGG GCGCCGCTGG TCTCTGGCTT CTCTCCCTTC CTCAGGCTAT 
GGGACAAATA CCCCAAGCTC CACCGTCTCC TCATCCTGTT CTTCCCAGGA GAAGTTGCAC 
CAGCTACCAT ACCAGCCAAC CCCAGATGAA TTACACTTCT TATCCAAACA CTTCTGCACA 
ACAGAAAGTA TCGCCACTGA GAACCGGTGC AGAAACACAC CCATGCGTCC ACGTTCCCGG 
AGTCTCAGCC CTGGACGGTC CCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCCACA GCTCAGATGG AAGAGCGTCT GAAGGAGATC 
ATCACCAGCT ACTCTCCAGA CCATGTTCTC CCCTTGGCAG ATGGGGTACT TAGTTTCACT 
CACCATCAGA TCATTGAGCT GGCTCGGGAC TGTTTGGATA AATCTCACCA GGGTCTCATC 
ACGTCGAGAT ACTTTTTTGA GTTGCAGCAC AAACTGGACA AGTTGCTCCA GGAGGCTCAC 
GATCGCTCTG AAAGTGGAGA ACTGGCATTT ATCAAGCAAC TAGTCCGAAA GATCCTAATT 
GTCATTGCCC GCCCCGCTCG GTTATTGGAG TGTTTGGAAT TTGATCCTGA AGAATTTTAT 
TACCTATTGG AAGCTGCAGA AGGCCATGCC AAAGAAGGCC AAGGAATCAA AACTGACATC 
CCTAGGTATA TCATCAGCCA GCTGGGACTC AATAAGGACC CTTTAGAAGA AATGGCTCAG 
TTGGGGAATT ACGACAGTAG GACAGCAGAG ACACCAGAGA TGGATGAGTC AGTGAGTAGC 
TCAAATACTT CCCTGAGACT TCGAAGGAAA CCCCGAGAGA GTGATTTTGA AACAATTAAA 
TTGATCAGCA ATGGAGCCTA CGGGGCAGTC TACTTTGTTC GGCACAAAGA GTCCCGCCAG 
AGGTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTCATCC TTCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGAGACAT CCTGACTTTC GCAGAGAACC CCTTTGTGGT CAGCATGTAT 
TGCTCCTTTG AAACGAGGCG TCACTTATGC ATGGTCATGG AGTATGTAGA AGGGGGAGAC 
TGTGCGACCC TAATGAAAAA CATGGGACCT CTCCCAGTTG ATATGGCCAG AATGTATTTC 
GCCGAGACCG TCTTGGCCTT GGAGTACCTG CATAATTACG GAATCGTACA CAGGGACTTG 
AAGCCAGACA ACCTGTTGGT CACCTCCATG GGGCACATAA AACTGACTGA CTTCGGCTTG 
TCTAAGGTGG GATTAATGAG CATGACCACC AATCTCTATG AAGGCCACAT AGAGAAGGAC 
GCTCGAGAGT TCTTAGATAA ACAGGTCTGT GGTACACCTG AGTACATTGC CCCCGAGGTG 
ATTCTGAGAC AGGGCTACGG GAAACCCGTG GACTGGTGGG CCATGGGCAT TATCCTCTAT 
GAATTTCTGG TCGGATGTGT GCCTTTCTTT GGGGACACTC CAGAAGAGCT ATTTGGACAA 
GTCATCAGTG ATGAAATCAA CTGGCCTGAA AAGGACGAGG CCCCTCCTCC AGACGCTCAG 
GAGCTGATTA CCTTGCTCCT CAGGCAGAAT CCGCTGGAGA GGCTGGGAAC AGGTGGAGCC 
TATGAAGTGA AGCAGCATCG TTTCTTCCGC TCCTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCGGAAT TTATTCCCCA ACTGGAATCG GAGGATGACA CAAGTTATTT TGATACTCGG 
TCAGAGAAGT ATCATCACAT GGAGACGGAG GAGGAGGACG ATACAAACGA TGAGGACTTC 
ACCGTGGAGA TAAGGCAGTT TTCTTCCTGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGCA TAACTCAAAA TTCAGGAGAA GACAAAGATG ACTCTGAGGA CAAGACCAAA 
AGCACAACGT TGCCATCCAC AGAGACACTC AGCTGGAGTT CCGAATACTC TGAAATGCAA 
CAGTTATCGA CCTCCAACTC TTCAGATACT GAAAGCAACA GGTGCAAACT CAGCTCTGGC 
TTGCTCCCCA AGCTGGCTAT TTCGACAGAT GGGGAACAAG ATGAGGCTGT CCCTTGCTCT 
GGAGACCCCA GAGAGGAGCC AGAGAAACCT GTCCCTCCCT CTGAGGAGTG TACTCAGGAG 
GAGCCCGAGG TCACCACCCC AGCCAGCACC ATCAGCAGTT CCACACTGTC AGATATGTTC 
GCTGTATCTC CATTGGGAAG CCCAATGTCC CCACACTCCC TGTCTTCAGA CCCTTCTTCT 
TCACGGGATT CCTCTCCCAG CCGAGACTCT TCTGCAGCAT CTGCCAGTCC GCATCAGCCC 
ATTGTCATCC ACAGCTCAGG CAAGAACTAT GGGTTCACCA TCCGTGCTAT CCGCGTGTAC 
GTGGGGGACA GTGACATCTA CACAGTGCAC CATATCGTCT GGAACGTAGA AGAAGGAAGT 
CCCGCATACC AGGCAGGACT GAAGGCCGGG GATCTGATCA CACACATCAA CGGAGAGCCG 
GTGCACGGCC TCGTCCACAC GGAAGTTATC GAGCTCCTGC TGAAGAGTGG GAATAAGGTG 
TCTATCACCA CTACTCCATT TGAAAACACA TCAATCAAAA CGGGACCAGC CAGGAGAAAC 
AGTTACAAGG GCCGGATGGT GAGACGAAGC AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA 
AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC AAGCAGCCTT CTCCTTTGCT CCACACCAGC 
CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG TCATCTGGAG AGAGCCTCCC GGGTTCCCCA 
ACTCACAGCT TGTCCCCGAG GTCTCCAACA CCCAGTTATC GTTCTACTCC CGATTTTCCG 
TCAGGTACAA ATTCCTCCCA GAGCAGCTCC CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA 
GGTTCAGGGC ACATCCGGCC CAGCACCCTC CATGGCCTGG CTCCCAAACT CAGCGGGCAG 
CGATACCGCT CTGGAAGACG GAAGTCGGCT GGCAGCATCC CTCTCTCCCC GCTGGCCAGG 
ACACCCTCTC CCACTCCACA GCCTACCTCT CCTCAGCGTT CACCATCCCC ACTGTTGGGA 
CACTCACTGG GCAATGCCAA GATCACTCAG GCCTTTCCTA GCAAGATGCA CTCTCCCCCC 
ACAATCGTCA GACACATCGT GAGGCCCAAG AGTGCAGAGC CGCCCCGCTC CCCACTGCTG 
AAACGGGTGC AGTCGGAGGA AAAGTTGTCA CCCTCCTATG GCAGTGACAA GAAGCTTCTG 
TGCTCCCGCA AGCATAGCCT AGAGGTGACA CAAGAGGAGG TACAGAGGGA GCAGTGTCAG 
CGGGAAGTGA CACTGCAGAG CCTGGAAGAG AATGTGTGTG ACGCTCCTTC CCTCAGTCGG 
GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA 
GAGTCTGTGG ATGACCTGGA CCGGGACAAG CTGAAAGCCA AGGTTGTCGT AAAGAAACCA 
GAAGAGAAAC ATGAATCGCA CCAGAAACCT CACAGCCTTG GTGGTGATTC GGAAAGCTAT 
GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA AAAGTGTACT CCAAGGGGTT GGAAAGGTCA 
GGCCATTTTG AAAACACATC AGCAGAGTTG CCTTCTGTGG GCAGCCTGCT GAAGGACACT 
CTTCACAAGC AGGCCAGCGT GAGGGCCAGC GAGGGGGTGA CCTCAGACGG GGCAGCTTGC 
AGCCTGACAC CAGGGGAGCA CAGCCAGTCT CTAGGTGACT TTAAGCGGGC CTCAGCTTCT 
GGCATTCTCC ATGATAGTGT GTGCCCCATC TCTGATAGGC CTGCTCCTGG AAAGGTTGAA 
TACTCGGAGA AGGCCTCTCA GGCCAAAGAG CTCCTTCGAA GTGAAAAACT AGACAGCAAG 
CTGGCCAATA TTGATTACCT CAGAAAGAAA ATGTCATTGG ATGACAAAGA TGACAGCCAC 
TGTGCCATCC TGAAACCCAA GATAACATCT AGCGCCCATG AATGTCTGCC AGGGAACCCC 
ATACGGCCCA TGGCAGGGCA ACAAGAGACC CCGCCAGCCT CTGAAAACCG GGCATTCATC 
AACAGTACCC ACACACCTCA GATGAGTGCA GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT 
GGCCGGGTAG AGAACGGAGG GGAGAAAGCA GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG 
AAGAGCCCCT CCGAGTATAA GCTAGAGGGC AGGTCAGTTT CATGTCTCAA GCCGATCGAG 
GGCACACTGG ACATTGCTCT CCTGTCTGGA CCTCACGCCT CCAAAACAGA GTTGCTTTCC 
CCAGAGCCTG CACAGAGTCC CAGCCCAGGC ATCAACGTGG GACCATGTGT GCCACTAGCT 
CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC TCCACCAGCC TGAGAGAGCC TTCCTCAGCC 
AACTTAAAAG TAAATAAATC TTATCTGCTG GAGCCTCGGT TCCTACCCCC GAGCCGGGCT 
CTCCAGGACT CTCTCGCAGC CTCTGGGCCA GAACCGAAGT CAAAGCCGGA AAGGAAGCTC 
ATTCATCCTT CTGCCAGGAG CCCAGCAACT GTCACAGAGA GCAATCTTCA GCAGAAAGAG 
GGTGGTCCCG CCACACACCA AGACCGCTCC ACTGACACCA GGAACCTCCC TGGCCCAGGG 
CAGACCCTAC ACAATGTGGA CCTACCCAGG CTGTGTACAC GTGCCCCACT CCCACCGGAA 
GGGACGCCCG CAAAGGAGAA GCCATGTCTG AAGGAACCCT CTGCCAAGGT GAAAAGCGAG 
TGGTCTGCCG TGAGGGATGA CGGACACAGA GATCCCTGTG CGAAGCTGTG CCCGGCAGAG 
ACTGGTAAAG CCAGCGACAG TTCCAAACCC CTGCCTTCCG GGGGGAGGAC CCAACCCGAT 
TTCTACAAGC AGACCCAGAC TTCGGAGAAA GCATGGGCGC ATGCAAAAAC AAACCACAAA 
GATAGCCAAG ATGAGGTGAA GTCCCTGGCC AGGGAGGACT CAGCTTCACT TTTATATGAA 
AAGGAGATAG GCCGGGCACG AAAAGGTCCT GAACCCAAAC CGGAAGTTCC TGCTACCCGG 
TGCCCTCCTC AGCCACCAGG AATTGAGGGT GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC 
TCTTTGCAGA AACAGGCTCC CAAAGAGCCA GACAGGAAGG AACAGACTTC GCAAAGGCCT 
GGAGGTAGTG GCCCTCAACA ACCCCCACCC ACCAAAGAGC TGTCTAACTC AGCATCCTGG 
CAGCACGGCA GTTCTCCGAG TCACACTTTA AAGAAGGAGC CCGGGACCAA AGCTGCCGCT 
GCAGAACCAA GCACCAGCCT TCATGACACT CCCCGATCTG CTACAGCCAC CACCACTGCC 
ATTGCCACCA CCACCACTAC CACCAGTGCC GGGCACAGTG ACTGCAGTAG CCATAAGGCC 
CGGCCTGGCC CTGACCCCAG CCCTTCAAAG TCTAAGCACC AAGACAGGTC CCTCTCCTCA 
CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC AAAGAGCCTG TCACTCAACC CCTGGGTGGT 
TCCATCAGAG AAGGCAAGGG TGGCAGCAAG GGTCCAGTGG ACACATTTTC TGCTGTCCTG 
ACCACCCAGG GCAAAGCAAG TGATGTGCTT GTGCAGGGAG AAGGTCGGGT CTCAATCATT 
GTCCACACTG AAGAGTGTCC TCTCGATGCC AAACTGAAAA ACACCAATGG AGGGTGTCCC 
CCAGAGATGC AGGCGAAGCA TCCACCCAGA CAAGGACATC TCAGTGAAGC AGCAGACCAG 
AAGCCACTCA TTGCTGGTGA GAAGCAAAGC CCGTCTCCAA AGCATCCCAA ACCATCCACT 
GTGAAAGATT ACCCCAGTCT GTGCAGACAG ACAGACAGAA GCCCAAGCCA TCAGGCTACC 
ACTGGGGACA GGAAGGCAGA AGGAAAGAAA TGCACAGACG CACTTTATGT CGCAGCCCCA 
GAGGGCTACA AGCCAGAGGC CAGCCCTTCT CTCCACCACG GCGAGACCGG ACTCAGAGGC 
TCAGAGAGGC CACCCATGGG CATGGGGAAG GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA 
GGTCCCCAGA AGTCACTGGC TGAAACAGGC AAGCCCAGCG GTATGAAAAG GTCACCCTCT 
GCCACCGTGC AGAGCTCTCT CCGCTCAGCT GCCCCCCCAG AAAAGTCTCT GAGTTACTCA 
GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC 
TCATCTGCCA AGGCTACAGG GGGGACCTCA GAGTTCCCAG CCCCCAGCAG CAGGGACCAC 
AGGAAGCTTC AGTCTGGAGG AGACGGCCGA AGCCAAATGA TAAAGAGTGA CTCTCTGCCC 
TCCTTCCGCC TCTCCACCTC TGCTCTGGAG TCACATTTCC AGGATCCACA GGTGCCCATC 
GCATCAGGCC ACCGAGGCAG GGCACTGTCA GTAACTGCTG CCACAGGAGA ACCCAAAGGG 
AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG AAACAGAATG CGTGCAGAGA GGCGACCAGA 
GCACCCCCAG CCCCAAGCAC AGATCGCTCC CTCCCTCTTT CCTCAGAGAA AGACTTCGTG 
GTTCGGCAGA GAAGGGGCAA GGAGACCTTA AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_006517701.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517764.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X7, mRNA.

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CloneID	OMu49077
Clone ID Related Accession (Same CDS sequence)	XM_006517701.3 , XM_006517701.4
Accession Version	XM_006517701.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517701.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGG AAGGGCAACC TCCGAAGAGC 
CATTTCAACT CAGCTCGACA TCGGCAGAGA CTAGTGGACC CAGCTGCTTC AAAAAAGGAG 
CTGAGCCTCC CCCGAAGAGG AAGTCTGGTA GATTCCCAGA AGTGGAATTG CTTGGTCAAA 
CGTTGCCGAA CAAGCAACCG GAAGAGTTTG ATAGGCAATG GCCAGTCTCC AGCACTGCCT 
CGACCACACT CACCTCTCTC TGCTCATGCA GGAAATAGCC CTCAAGACAG TCCAAGGAAT 
TTCTCCCCCA GTGCCTCAGC CCATTTCTCA TTTGCGAGGA GAAATGACAG AACGGATGGG 
CGCCGCTGGT CTCTGGCTTC TCTCCCTTCC TCAGGCTATG GGACAAATAC CCCAAGCTCC 
ACCGTCTCCT CATCCTGTTC TTCCCAGGAG AAGTTGCACC AGCTACCATA CCAGCCAACC 
CCAGATGAAT TACACTTCTT ATCCAAACAC TTCTGCACAA CAGAAAGTAT CGCCACTGAG 
AACCGGTGCA GAAACACACC CATGCGTCCA CGTTCCCGGA GTCTCAGCCC TGGACGGTCC 
CCCGCCTGCT GTGACCATGA AATAATTATG ATGAACCATG TCTACAAAGA AAGGTTCCCA 
AAGGCCACAG CTCAGATGGA AGAGCGTCTG AAGGAGATCA TCACCAGCTA CTCTCCAGAC 
CATGTTCTCC CCTTGGCAGA TGGGGTACTT AGTTTCACTC ACCATCAGAT CATTGAGCTG 
GCTCGGGACT GTTTGGATAA ATCTCACCAG GGTCTCATCA CGTCGAGATA CTTTTTTGAG 
TTGCAGCACA AACTGGACAA GTTGCTCCAG GAGGCTCACG ATCGCTCTGA AAGTGGAGAA 
CTGGCATTTA TCAAGCAACT AGTCCGAAAG ATCCTAATTG TCATTGCCCG CCCCGCTCGG 
TTATTGGAGT GTTTGGAATT TGATCCTGAA GAATTTTATT ACCTATTGGA AGCTGCAGAA 
GGCCATGCCA AAGAAGGCCA AGGAATCAAA ACTGACATCC CTAGGTATAT CATCAGCCAG 
CTGGGACTCA ATAAGGACCC TTTAGAAGAA ATGGCTCAGT TGGGGAATTA CGACAGTAGG 
ACAGCAGAGA CACCAGAGAT GGATGAGTCA GTGAGTAGCT CAAATACTTC CCTGAGACTT 
CGAAGGAAAC CCCGAGAGAG TGATTTTGAA ACAATTAAAT TGATCAGCAA TGGAGCCTAC 
GGGGCAGTCT ACTTTGTTCG GCACAAAGAG TCCCGCCAGA GGTTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTCATCCT TCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGAGACATC 
CTGACTTTCG CAGAGAACCC CTTTGTGGTC AGCATGTATT GCTCCTTTGA AACGAGGCGT 
CACTTATGCA TGGTCATGGA GTATGTAGAA GGGGGAGACT GTGCGACCCT AATGAAAAAC 
ATGGGACCTC TCCCAGTTGA TATGGCCAGA ATGTATTTCG CCGAGACCGT CTTGGCCTTG 
GAGTACCTGC ATAATTACGG AATCGTACAC AGGGACTTGA AGCCAGACAA CCTGTTGGTC 
ACCTCCATGG GGCACATAAA ACTGACTGAC TTCGGCTTGT CTAAGGTGGG ATTAATGAGC 
ATGACCACCA ATCTCTATGA AGGCCACATA GAGAAGGACG CTCGAGAGTT CTTAGATAAA 
CAGGTCTGTG GTACACCTGA GTACATTGCC CCCGAGGTGA TTCTGAGACA GGGCTACGGG 
AAACCCGTGG ACTGGTGGGC CATGGGCATT ATCCTCTATG AATTTCTGGT CGGATGTGTG 
CCTTTCTTTG GGGACACTCC AGAAGAGCTA TTTGGACAAG TCATCAGTGA TGAAATCAAC 
TGGCCTGAAA AGGACGAGGC CCCTCCTCCA GACGCTCAGG AGCTGATTAC CTTGCTCCTC 
AGGCAGAATC CGCTGGAGAG GCTGGGAACA GGTGGAGCCT ATGAAGTGAA GCAGCATCGT 
TTCTTCCGCT CCTTAGACTG GAACAGTTTG CTGAGACAGA AGGCGGAATT TATTCCCCAA 
CTGGAATCGG AGGATGACAC AAGTTATTTT GATACTCGGT CAGAGAAGTA TCATCACATG 
GAGACGGAGG AGGAGGACGA TACAAACGAT GAGGACTTCA CCGTGGAGAT AAGGCAGTTT 
TCTTCCTGTT CACACAGGTT TTCAAAAGTT TTCAGCAGTA TAGATCGCAT AACTCAAAAT 
TCAGGAGAAG ACAAAGATGA CTCTGAGGAC AAGACCAAAA GCACAACGTT GCCATCCACA 
GAGACACTCA GCTGGAGTTC CGAATACTCT GAAATGCAAC AGTTATCGAC CTCCAACTCT 
TCAGATACTG AAAGCAACAG GTGCAAACTC AGCTCTGGCT TGCTCCCCAA GCTGGCTATT 
TCGACAGATG GGGAACAAGA TGAGGCTGTC CCTTGCTCTG GAGACCCCAG AGAGGAGCCA 
GAGAAACCTG TCCCTCCCTC TGAGGAGTGT ACTCAGGAGG AGCCCGAGGT CACCACCCCA 
GCCAGCACCA TCAGCAGTTC CACACTGTCA GTTGGCAGTT TTTCAGAGCA CTTGGATCAG 
ATAAATGGGC GAAGCGAGTG TGTGGACAGT ACAGATAATT CCTCAAAGCC ATCCAGTGAA 
CCCACTTCTC ACGTGGCTCG ACAGCGCTTA GAAAGCACAG AGAAAAAGAA AATTTCTGGG 
AAAGTCACAA AGTCCCTCTC GGCCAGTGCT CTGTCCCTCA TGATCCCAGG AGATATGTTC 
GCTGTATCTC CATTGGGAAG CCCAATGTCC CCACACTCCC TGTCTTCAGA CCCTTCTTCT 
TCACGGGATT CCTCTCCCAG CCGAGACTCT TCTGCAGCAT CTGCCAGTCC GCATCAGCCC 
ATTGTCATCC ACAGCTCAGG CAAGAACTAT GGGTTCACCA TCCGTGCTAT CCGCGTGTAC 
GTGGGGGACA GTGACATCTA CACAGTGCAC CATATCGTCT GGAACGTAGA AGAAGGAAGT 
CCCGCATACC AGGCAGGACT GAAGGCCGGG GATCTGATCA CACACATCAA CGGAGAGCCG 
GTGCACGGCC TCGTCCACAC GGAAGTTATC GAGCTCCTGC TGAAGAGTGG GAATAAGGTG 
TCTATCACCA CTACTCCATT TGAAAACACA TCAATCAAAA CGGGACCAGC CAGGAGAAAC 
AGTTACAAGG GCCGGATGGT GAGACGAAGC AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA 
AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC AAGCAGCCTT CTCCTTTGCT CCACACCAGC 
CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG TCATCTGGAG AGAGCCTCCC GGGTTCCCCA 
ACTCACAGCT TGTCCCCGAG GTCTCCAACA CCCAGTTATC GTTCTACTCC CGATTTTCCG 
TCAGGTACAA ATTCCTCCCA GAGCAGCTCC CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA 
GGTTCAGGGC ACATCCGGCC CAGCACCCTC CATGGCCTGG CTCCCAAACT CAGCGGGCAG 
CGATACCGCT CTGGAAGACG GAAGTCGGCT GGCAGCATCC CTCTCTCCCC GCTGGCCAGG 
ACACCCTCTC CCACTCCACA GCCTACCTCT CCTCAGCGTT CACCATCCCC ACTGTTGGGA 
CACTCACTGG GCAATGCCAA GATCACTCAG GCCTTTCCTA GCAAGATGCA CTCTCCCCCC 
ACAATCGTCA GACACATCGT GAGGCCCAAG AGTGCAGAGC CGCCCCGCTC CCCACTGCTG 
AAACGGGTGC AGTCGGAGGA AAAGTTGTCA CCCTCCTATG GCAGTGACAA GAAGCTTCTG 
TGCTCCCGCA AGCATAGCCT AGAGGTGACA CAAGAGGAGG TACAGAGGGA GCAGTGTCAG 
CGGGAAGTGA CACTGCAGAG CCTGGAAGAG AATGTGTGTG ACGCTCCTTC CCTCAGTCGG 
GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA 
GAGTCTGTGG ATGACCTGGA CCGGGACAAG CTGAAAGCCA AGGTTGTCGT AAAGAAACCA 
GAAGAGAAAC ATGAATCGCA CCAGAAACCT CACAGCCTTG GTGGTGATTC GGAAAGCTAT 
GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA AAAGTGTACT CCAAGGGGTT GGAAAGGTCA 
GGCCATTTTG AAAACACATC AGCAGAGTTG CCTTCTGTGG GCAGCCTGCT GAAGGACACT 
CTTCACAAGC AGGCCAGCGT GAGGGCCAGC GAGGGGGTGA CCTCAGACGG GGCAGCTTGC 
AGCCTGACAC CAGGGGAGCA CAGCCAGTCT CTAGGTGACT TTAAGCGGGC CTCAGCTTCT 
GGCATTCTCC ATGATAGTGT GTGCCCCATC TCTGATAGGC CTGCTCCTGG AAAGGTTGAA 
TACTCGGAGA AGGCCTCTCA GGCCAAAGAG CTCCTTCGAA GTGAAAAACT AGACAGCAAG 
CTGGCCAATA TTGATTACCT CAGAAAGAAA ATGTCATTGG ATGACAAAGA TGACAGCCAC 
TGTGCCATCC TGAAACCCAA GATAACATCT AGCGCCCATG AATGTCTGCC AGGGAACCCC 
ATACGGCCCA TGGCAGGGCA ACAAGAGACC CCGCCAGCCT CTGAAAACCG GGCATTCATC 
AACAGTACCC ACACACCTCA GATGAGTGCA GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT 
GGCCGGGTAG AGAACGGAGG GGAGAAAGCA GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG 
AAGAGCCCCT CCGAGTATAA GCTAGAGGGC AGGTCAGTTT CATGTCTCAA GCCGATCGAG 
GGCACACTGG ACATTGCTCT CCTGTCTGGA CCTCACGCCT CCAAAACAGA GTTGCTTTCC 
CCAGAGCCTG CACAGAGTCC CAGCCCAGGC ATCAACGTGG GACCATGTGT GCCACTAGCT 
CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC TCCACCAGCC TGAGAGAGCC TTCCTCAGCC 
AACTTAAAAG TAAATAAATC TTATCTGCTG GAGCCTCGGT TCCTACCCCC GAGCCGGGCT 
CTCCAGGACT CTCTCGCAGC CTCTGGGCCA GAACCGAAGT CAAAGCCGGA AAGGAAGCTC 
ATTCATCCTT CTGCCAGGAG CCCAGCAACT GTCACAGAGA GCAATCTTCA GCAGAAAGAG 
GGTGGTCCCG CCACACACCA AGACCGCTCC ACTGACACCA GGAACCTCCC TGGCCCAGGG 
CAGACCCTAC ACAATGTGGA CCTACCCAGG CTGTGTACAC GTGCCCCACT CCCACCGGAA 
GGGACGCCCG CAAAGGAGAA GCCATGTCTG AAGGAACCCT CTGCCAAGGT GAAAAGCGAG 
TGGTCTGCCG TGAGGGATGA CGGACACAGA GATCCCTGTG CGAAGCTGTG CCCGGCAGAG 
ACTGGTAAAG CCAGCGACAG TTCCAAACCC CTGCCTTCCG GGGGGAGGAC CCAACCCGAT 
TTCTACAAGC AGACCCAGAC TTCGGAGAAA GCATGGGCGC ATGCAAAAAC AAACCACAAA 
GATAGCCAAG ATGAGGTGAA GTCCCTGGCC AGGGAGGACT CAGCTTCACT TTTATATGAA 
AAGGAGATAG GCCGGGCACG AAAAGGTCCT GAACCCAAAC CGGAAGTTCC TGCTACCCGG 
TGCCCTCCTC AGCCACCAGG AATTGAGGGT GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC 
TCTTTGCAGA AACAGGCTCC CAAAGAGCCA GACAGGAAGG AACAGACTTC GCAAAGGCCT 
GGAGGTAGTG GCCCTCAACA ACCCCCACCC ACCAAAGAGC TGTCTAACTC AGCATCCTGG 
CAGCACGGCA GTTCTCCGAG TCACACTTTA AAGAAGGAGC CCGGGACCAA AGCTGCCGCT 
GCAGAACCAA GCACCAGCCT TCATGACACT CCCCGATCTG CTACAGCCAC CACCACTGCC 
ATTGCCACCA CCACCACTAC CACCAGTGCC GGGCACAGTG ACTGCAGTAG CCATAAGGCC 
CGGCCTGGCC CTGACCCCAG CCCTTCAAAG TCTAAGCACC AAGACAGGTC CCTCTCCTCA 
CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC AAAGAGCCTG TCACTCAACC CCTGGGTGGT 
TCCATCAGAG AAGGCAAGGG TGGCAGCAAG GGTCCAGTGG ACACATTTTC TGCTGTCCTG 
ACCACCCAGG GCAAAGCAAG TGATGTGCTT GTGCAGGGAG AAGGTCGGGT CTCAATCATT 
GTCCACACTG AAGAGTGTCC TCTCGATGCC AAACTGAAAA ACACCAATGG AGGGTGTCCC 
CCAGAGATGC AGGCGAAGCA TCCACCCAGA CAAGGACATC TCAGTGAAGC AGCAGACCAG 
AAGCCACTCA TTGCTGGTGA GAAGCAAAGC CCGTCTCCAA AGCATCCCAA ACCATCCACT 
GTGAAAGATT ACCCCAGTCT GTGCAGACAG ACAGACAGAA GCCCAAGCCA TCAGGCTACC 
ACTGGGGACA GGAAGGCAGA AGGAAAGAAA TGCACAGACG CACTTTATGT CGCAGCCCCA 
GAGGGCTACA AGCCAGAGGC CAGCCCTTCT CTCCACCACG GCGAGACCGG ACTCAGAGGC 
TCAGAGAGGC CACCCATGGG CATGGGGAAG GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA 
GGTCCCCAGA AGTCACTGGC TGAAACAGGC AAGCCCAGCG GTATGAAAAG GTCACCCTCT 
GCCACCGTGC AGAGCTCTCT CCGCTCAGCT GCCCCCCCAG AAAAGTCTCT GAGTTACTCA 
GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC 
TCATCTGCCA AGGCTACAGG GGGGACCTCA GAGTTCCCAG CCCCCAGCAG CAGGGACCAC 
AGGAAGCTTC AGTCTGGAGG AGACGGCCGA AGCCAAATGA TAAAGAGTGA CTCTCTGCCC 
TCCTTCCGCC TCTCCACCTC TGCTCTGGAG TCACATTTCC AGGATCCACA GGTGCCCATC 
GCATCAGGCC ACCGAGGCAG GGCACTGTCA GTAACTGCTG CCACAGGAGA ACCCAAAGGG 
AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG AAACAGAATG CGTGCAGAGA GGCGACCAGA 
GCACCCCCAG CCCCAAGCAC AGATCGCTCC CTCCCTCTTT CCTCAGAGAA AGACTTCGTG 
GTTCGGCAGA GAAGGGGCAA GGAGACCTTA AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517764.1
CDS	475..7854
Translation	MDESSILRRRGLQEGQPPKSHFNSARHRQRLVDPAASKKELSLPRRGSLVDSQKWNCLVKRCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_006517701.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517701.4

ORF Insert Sequence:

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGG AAGGGCAACC TCCGAAGAGC 
CATTTCAACT CAGCTCGACA TCGGCAGAGA CTAGTGGACC CAGCTGCTTC AAAAAAGGAG 
CTGAGCCTCC CCCGAAGAGG AAGTCTGGTA GATTCCCAGA AGTGGAATTG CTTGGTCAAA 
CGTTGCCGAA CAAGCAACCG GAAGAGTTTG ATAGGCAATG GCCAGTCTCC AGCACTGCCT 
CGACCACACT CACCTCTCTC TGCTCATGCA GGAAATAGCC CTCAAGACAG TCCAAGGAAT 
TTCTCCCCCA GTGCCTCAGC CCATTTCTCA TTTGCGAGGA GAAATGACAG AACGGATGGG 
CGCCGCTGGT CTCTGGCTTC TCTCCCTTCC TCAGGCTATG GGACAAATAC CCCAAGCTCC 
ACCGTCTCCT CATCCTGTTC TTCCCAGGAG AAGTTGCACC AGCTACCATA CCAGCCAACC 
CCAGATGAAT TACACTTCTT ATCCAAACAC TTCTGCACAA CAGAAAGTAT CGCCACTGAG 
AACCGGTGCA GAAACACACC CATGCGTCCA CGTTCCCGGA GTCTCAGCCC TGGACGGTCC 
CCCGCCTGCT GTGACCATGA AATAATTATG ATGAACCATG TCTACAAAGA AAGGTTCCCA 
AAGGCCACAG CTCAGATGGA AGAGCGTCTG AAGGAGATCA TCACCAGCTA CTCTCCAGAC 
CATGTTCTCC CCTTGGCAGA TGGGGTACTT AGTTTCACTC ACCATCAGAT CATTGAGCTG 
GCTCGGGACT GTTTGGATAA ATCTCACCAG GGTCTCATCA CGTCGAGATA CTTTTTTGAG 
TTGCAGCACA AACTGGACAA GTTGCTCCAG GAGGCTCACG ATCGCTCTGA AAGTGGAGAA 
CTGGCATTTA TCAAGCAACT AGTCCGAAAG ATCCTAATTG TCATTGCCCG CCCCGCTCGG 
TTATTGGAGT GTTTGGAATT TGATCCTGAA GAATTTTATT ACCTATTGGA AGCTGCAGAA 
GGCCATGCCA AAGAAGGCCA AGGAATCAAA ACTGACATCC CTAGGTATAT CATCAGCCAG 
CTGGGACTCA ATAAGGACCC TTTAGAAGAA ATGGCTCAGT TGGGGAATTA CGACAGTAGG 
ACAGCAGAGA CACCAGAGAT GGATGAGTCA GTGAGTAGCT CAAATACTTC CCTGAGACTT 
CGAAGGAAAC CCCGAGAGAG TGATTTTGAA ACAATTAAAT TGATCAGCAA TGGAGCCTAC 
GGGGCAGTCT ACTTTGTTCG GCACAAAGAG TCCCGCCAGA GGTTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTCATCCT TCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGAGACATC 
CTGACTTTCG CAGAGAACCC CTTTGTGGTC AGCATGTATT GCTCCTTTGA AACGAGGCGT 
CACTTATGCA TGGTCATGGA GTATGTAGAA GGGGGAGACT GTGCGACCCT AATGAAAAAC 
ATGGGACCTC TCCCAGTTGA TATGGCCAGA ATGTATTTCG CCGAGACCGT CTTGGCCTTG 
GAGTACCTGC ATAATTACGG AATCGTACAC AGGGACTTGA AGCCAGACAA CCTGTTGGTC 
ACCTCCATGG GGCACATAAA ACTGACTGAC TTCGGCTTGT CTAAGGTGGG ATTAATGAGC 
ATGACCACCA ATCTCTATGA AGGCCACATA GAGAAGGACG CTCGAGAGTT CTTAGATAAA 
CAGGTCTGTG GTACACCTGA GTACATTGCC CCCGAGGTGA TTCTGAGACA GGGCTACGGG 
AAACCCGTGG ACTGGTGGGC CATGGGCATT ATCCTCTATG AATTTCTGGT CGGATGTGTG 
CCTTTCTTTG GGGACACTCC AGAAGAGCTA TTTGGACAAG TCATCAGTGA TGAAATCAAC 
TGGCCTGAAA AGGACGAGGC CCCTCCTCCA GACGCTCAGG AGCTGATTAC CTTGCTCCTC 
AGGCAGAATC CGCTGGAGAG GCTGGGAACA GGTGGAGCCT ATGAAGTGAA GCAGCATCGT 
TTCTTCCGCT CCTTAGACTG GAACAGTTTG CTGAGACAGA AGGCGGAATT TATTCCCCAA 
CTGGAATCGG AGGATGACAC AAGTTATTTT GATACTCGGT CAGAGAAGTA TCATCACATG 
GAGACGGAGG AGGAGGACGA TACAAACGAT GAGGACTTCA CCGTGGAGAT AAGGCAGTTT 
TCTTCCTGTT CACACAGGTT TTCAAAAGTT TTCAGCAGTA TAGATCGCAT AACTCAAAAT 
TCAGGAGAAG ACAAAGATGA CTCTGAGGAC AAGACCAAAA GCACAACGTT GCCATCCACA 
GAGACACTCA GCTGGAGTTC CGAATACTCT GAAATGCAAC AGTTATCGAC CTCCAACTCT 
TCAGATACTG AAAGCAACAG GTGCAAACTC AGCTCTGGCT TGCTCCCCAA GCTGGCTATT 
TCGACAGATG GGGAACAAGA TGAGGCTGTC CCTTGCTCTG GAGACCCCAG AGAGGAGCCA 
GAGAAACCTG TCCCTCCCTC TGAGGAGTGT ACTCAGGAGG AGCCCGAGGT CACCACCCCA 
GCCAGCACCA TCAGCAGTTC CACACTGTCA GTTGGCAGTT TTTCAGAGCA CTTGGATCAG 
ATAAATGGGC GAAGCGAGTG TGTGGACAGT ACAGATAATT CCTCAAAGCC ATCCAGTGAA 
CCCACTTCTC ACGTGGCTCG ACAGCGCTTA GAAAGCACAG AGAAAAAGAA AATTTCTGGG 
AAAGTCACAA AGTCCCTCTC GGCCAGTGCT CTGTCCCTCA TGATCCCAGG AGATATGTTC 
GCTGTATCTC CATTGGGAAG CCCAATGTCC CCACACTCCC TGTCTTCAGA CCCTTCTTCT 
TCACGGGATT CCTCTCCCAG CCGAGACTCT TCTGCAGCAT CTGCCAGTCC GCATCAGCCC 
ATTGTCATCC ACAGCTCAGG CAAGAACTAT GGGTTCACCA TCCGTGCTAT CCGCGTGTAC 
GTGGGGGACA GTGACATCTA CACAGTGCAC CATATCGTCT GGAACGTAGA AGAAGGAAGT 
CCCGCATACC AGGCAGGACT GAAGGCCGGG GATCTGATCA CACACATCAA CGGAGAGCCG 
GTGCACGGCC TCGTCCACAC GGAAGTTATC GAGCTCCTGC TGAAGAGTGG GAATAAGGTG 
TCTATCACCA CTACTCCATT TGAAAACACA TCAATCAAAA CGGGACCAGC CAGGAGAAAC 
AGTTACAAGG GCCGGATGGT GAGACGAAGC AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA 
AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC AAGCAGCCTT CTCCTTTGCT CCACACCAGC 
CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG TCATCTGGAG AGAGCCTCCC GGGTTCCCCA 
ACTCACAGCT TGTCCCCGAG GTCTCCAACA CCCAGTTATC GTTCTACTCC CGATTTTCCG 
TCAGGTACAA ATTCCTCCCA GAGCAGCTCC CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA 
GGTTCAGGGC ACATCCGGCC CAGCACCCTC CATGGCCTGG CTCCCAAACT CAGCGGGCAG 
CGATACCGCT CTGGAAGACG GAAGTCGGCT GGCAGCATCC CTCTCTCCCC GCTGGCCAGG 
ACACCCTCTC CCACTCCACA GCCTACCTCT CCTCAGCGTT CACCATCCCC ACTGTTGGGA 
CACTCACTGG GCAATGCCAA GATCACTCAG GCCTTTCCTA GCAAGATGCA CTCTCCCCCC 
ACAATCGTCA GACACATCGT GAGGCCCAAG AGTGCAGAGC CGCCCCGCTC CCCACTGCTG 
AAACGGGTGC AGTCGGAGGA AAAGTTGTCA CCCTCCTATG GCAGTGACAA GAAGCTTCTG 
TGCTCCCGCA AGCATAGCCT AGAGGTGACA CAAGAGGAGG TACAGAGGGA GCAGTGTCAG 
CGGGAAGTGA CACTGCAGAG CCTGGAAGAG AATGTGTGTG ACGCTCCTTC CCTCAGTCGG 
GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA 
GAGTCTGTGG ATGACCTGGA CCGGGACAAG CTGAAAGCCA AGGTTGTCGT AAAGAAACCA 
GAAGAGAAAC ATGAATCGCA CCAGAAACCT CACAGCCTTG GTGGTGATTC GGAAAGCTAT 
GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA AAAGTGTACT CCAAGGGGTT GGAAAGGTCA 
GGCCATTTTG AAAACACATC AGCAGAGTTG CCTTCTGTGG GCAGCCTGCT GAAGGACACT 
CTTCACAAGC AGGCCAGCGT GAGGGCCAGC GAGGGGGTGA CCTCAGACGG GGCAGCTTGC 
AGCCTGACAC CAGGGGAGCA CAGCCAGTCT CTAGGTGACT TTAAGCGGGC CTCAGCTTCT 
GGCATTCTCC ATGATAGTGT GTGCCCCATC TCTGATAGGC CTGCTCCTGG AAAGGTTGAA 
TACTCGGAGA AGGCCTCTCA GGCCAAAGAG CTCCTTCGAA GTGAAAAACT AGACAGCAAG 
CTGGCCAATA TTGATTACCT CAGAAAGAAA ATGTCATTGG ATGACAAAGA TGACAGCCAC 
TGTGCCATCC TGAAACCCAA GATAACATCT AGCGCCCATG AATGTCTGCC AGGGAACCCC 
ATACGGCCCA TGGCAGGGCA ACAAGAGACC CCGCCAGCCT CTGAAAACCG GGCATTCATC 
AACAGTACCC ACACACCTCA GATGAGTGCA GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT 
GGCCGGGTAG AGAACGGAGG GGAGAAAGCA GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG 
AAGAGCCCCT CCGAGTATAA GCTAGAGGGC AGGTCAGTTT CATGTCTCAA GCCGATCGAG 
GGCACACTGG ACATTGCTCT CCTGTCTGGA CCTCACGCCT CCAAAACAGA GTTGCTTTCC 
CCAGAGCCTG CACAGAGTCC CAGCCCAGGC ATCAACGTGG GACCATGTGT GCCACTAGCT 
CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC TCCACCAGCC TGAGAGAGCC TTCCTCAGCC 
AACTTAAAAG TAAATAAATC TTATCTGCTG GAGCCTCGGT TCCTACCCCC GAGCCGGGCT 
CTCCAGGACT CTCTCGCAGC CTCTGGGCCA GAACCGAAGT CAAAGCCGGA AAGGAAGCTC 
ATTCATCCTT CTGCCAGGAG CCCAGCAACT GTCACAGAGA GCAATCTTCA GCAGAAAGAG 
GGTGGTCCCG CCACACACCA AGACCGCTCC ACTGACACCA GGAACCTCCC TGGCCCAGGG 
CAGACCCTAC ACAATGTGGA CCTACCCAGG CTGTGTACAC GTGCCCCACT CCCACCGGAA 
GGGACGCCCG CAAAGGAGAA GCCATGTCTG AAGGAACCCT CTGCCAAGGT GAAAAGCGAG 
TGGTCTGCCG TGAGGGATGA CGGACACAGA GATCCCTGTG CGAAGCTGTG CCCGGCAGAG 
ACTGGTAAAG CCAGCGACAG TTCCAAACCC CTGCCTTCCG GGGGGAGGAC CCAACCCGAT 
TTCTACAAGC AGACCCAGAC TTCGGAGAAA GCATGGGCGC ATGCAAAAAC AAACCACAAA 
GATAGCCAAG ATGAGGTGAA GTCCCTGGCC AGGGAGGACT CAGCTTCACT TTTATATGAA 
AAGGAGATAG GCCGGGCACG AAAAGGTCCT GAACCCAAAC CGGAAGTTCC TGCTACCCGG 
TGCCCTCCTC AGCCACCAGG AATTGAGGGT GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC 
TCTTTGCAGA AACAGGCTCC CAAAGAGCCA GACAGGAAGG AACAGACTTC GCAAAGGCCT 
GGAGGTAGTG GCCCTCAACA ACCCCCACCC ACCAAAGAGC TGTCTAACTC AGCATCCTGG 
CAGCACGGCA GTTCTCCGAG TCACACTTTA AAGAAGGAGC CCGGGACCAA AGCTGCCGCT 
GCAGAACCAA GCACCAGCCT TCATGACACT CCCCGATCTG CTACAGCCAC CACCACTGCC 
ATTGCCACCA CCACCACTAC CACCAGTGCC GGGCACAGTG ACTGCAGTAG CCATAAGGCC 
CGGCCTGGCC CTGACCCCAG CCCTTCAAAG TCTAAGCACC AAGACAGGTC CCTCTCCTCA 
CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC AAAGAGCCTG TCACTCAACC CCTGGGTGGT 
TCCATCAGAG AAGGCAAGGG TGGCAGCAAG GGTCCAGTGG ACACATTTTC TGCTGTCCTG 
ACCACCCAGG GCAAAGCAAG TGATGTGCTT GTGCAGGGAG AAGGTCGGGT CTCAATCATT 
GTCCACACTG AAGAGTGTCC TCTCGATGCC AAACTGAAAA ACACCAATGG AGGGTGTCCC 
CCAGAGATGC AGGCGAAGCA TCCACCCAGA CAAGGACATC TCAGTGAAGC AGCAGACCAG 
AAGCCACTCA TTGCTGGTGA GAAGCAAAGC CCGTCTCCAA AGCATCCCAA ACCATCCACT 
GTGAAAGATT ACCCCAGTCT GTGCAGACAG ACAGACAGAA GCCCAAGCCA TCAGGCTACC 
ACTGGGGACA GGAAGGCAGA AGGAAAGAAA TGCACAGACG CACTTTATGT CGCAGCCCCA 
GAGGGCTACA AGCCAGAGGC CAGCCCTTCT CTCCACCACG GCGAGACCGG ACTCAGAGGC 
TCAGAGAGGC CACCCATGGG CATGGGGAAG GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA 
GGTCCCCAGA AGTCACTGGC TGAAACAGGC AAGCCCAGCG GTATGAAAAG GTCACCCTCT 
GCCACCGTGC AGAGCTCTCT CCGCTCAGCT GCCCCCCCAG AAAAGTCTCT GAGTTACTCA 
GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC 
TCATCTGCCA AGGCTACAGG GGGGACCTCA GAGTTCCCAG CCCCCAGCAG CAGGGACCAC 
AGGAAGCTTC AGTCTGGAGG AGACGGCCGA AGCCAAATGA TAAAGAGTGA CTCTCTGCCC 
TCCTTCCGCC TCTCCACCTC TGCTCTGGAG TCACATTTCC AGGATCCACA GGTGCCCATC 
GCATCAGGCC ACCGAGGCAG GGCACTGTCA GTAACTGCTG CCACAGGAGA ACCCAAAGGG 
AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG AAACAGAATG CGTGCAGAGA GGCGACCAGA 
GCACCCCCAG CCCCAAGCAC AGATCGCTCC CTCCCTCTTT CCTCAGAGAA AGACTTCGTG 
GTTCGGCAGA GAAGGGGCAA GGAGACCTTA AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_006517702.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517765.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X11, mRNA.

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CloneID	OMu49078
Clone ID Related Accession (Same CDS sequence)	XM_006517702.3 , XM_006517702.4
Accession Version	XM_006517702.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517702.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGA AGGAGCTGAG CCTCCCCCGA 
AGAGGAAGTC TGGTAGATTC CCAGAAGTGG AATTGCTTGG TCAAACGTTG CCGAACAAGC 
AACCGGAAGA GTTTGATAGG CAATGGCCAG TCTCCAGCAC TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GACAGTCCAA GGAATTTCTC CCCCAGTGCC 
TCAGCCCATT TCTCATTTGC GAGGAGAAAT GACAGAACGG ATGGGCGCCG CTGGTCTCTG 
GCTTCTCTCC CTTCCTCAGG CTATGGGACA AATACCCCAA GCTCCACCGT CTCCTCATCC 
TGTTCTTCCC AGGAGAAGTT GCACCAGCTA CCATACCAGC CAACCCCAGA TGAATTACAC 
TTCTTATCCA AACACTTCTG CACAACAGAA AGTATCGCCA CTGAGAACCG GTGCAGAAAC 
ACACCCATGC GTCCACGTTC CCGGAGTCTC AGCCCTGGAC GGTCCCCCGC CTGCTGTGAC 
CATGAAATAA TTATGATGAA CCATGTCTAC AAAGAAAGGT TCCCAAAGGC CACAGCTCAG 
ATGGAAGAGC GTCTGAAGGA GATCATCACC AGCTACTCTC CAGACCATGT TCTCCCCTTG 
GCAGATGGGG TACTTAGTTT CACTCACCAT CAGATCATTG AGCTGGCTCG GGACTGTTTG 
GATAAATCTC ACCAGGGTCT CATCACGTCG AGATACTTTT TTGAGTTGCA GCACAAACTG 
GACAAGTTGC TCCAGGAGGC TCACGATCGC TCTGAAAGTG GAGAACTGGC ATTTATCAAG 
CAACTAGTCC GAAAGATCCT AATTGTCATT GCCCGCCCCG CTCGGTTATT GGAGTGTTTG 
GAATTTGATC CTGAAGAATT TTATTACCTA TTGGAAGCTG CAGAAGGCCA TGCCAAAGAA 
GGCCAAGGAA TCAAAACTGA CATCCCTAGG TATATCATCA GCCAGCTGGG ACTCAATAAG 
GACCCTTTAG AAGAAATGGC TCAGTTGGGG AATTACGACA GTAGGACAGC AGAGACACCA 
GAGATGGATG AGTCAGTGAG TAGCTCAAAT ACTTCCCTGA GACTTCGAAG GAAACCCCGA 
GAGAGTGATT TTGAAACAAT TAAATTGATC AGCAATGGAG CCTACGGGGC AGTCTACTTT 
GTTCGGCACA AAGAGTCCCG CCAGAGGTTT GCCATGAAGA AGATCAACAA GCAGAACCTC 
ATCCTTCGGA ACCAGATCCA GCAGGCCTTC GTGGAGCGAG ACATCCTGAC TTTCGCAGAG 
AACCCCTTTG TGGTCAGCAT GTATTGCTCC TTTGAAACGA GGCGTCACTT ATGCATGGTC 
ATGGAGTATG TAGAAGGGGG AGACTGTGCG ACCCTAATGA AAAACATGGG ACCTCTCCCA 
GTTGATATGG CCAGAATGTA TTTCGCCGAG ACCGTCTTGG CCTTGGAGTA CCTGCATAAT 
TACGGAATCG TACACAGGGA CTTGAAGCCA GACAACCTGT TGGTCACCTC CATGGGGCAC 
ATAAAACTGA CTGACTTCGG CTTGTCTAAG GTGGGATTAA TGAGCATGAC CACCAATCTC 
TATGAAGGCC ACATAGAGAA GGACGCTCGA GAGTTCTTAG ATAAACAGGT CTGTGGTACA 
CCTGAGTACA TTGCCCCCGA GGTGATTCTG AGACAGGGCT ACGGGAAACC CGTGGACTGG 
TGGGCCATGG GCATTATCCT CTATGAATTT CTGGTCGGAT GTGTGCCTTT CTTTGGGGAC 
ACTCCAGAAG AGCTATTTGG ACAAGTCATC AGTGATGAAA TCAACTGGCC TGAAAAGGAC 
GAGGCCCCTC CTCCAGACGC TCAGGAGCTG ATTACCTTGC TCCTCAGGCA GAATCCGCTG 
GAGAGGCTGG GAACAGGTGG AGCCTATGAA GTGAAGCAGC ATCGTTTCTT CCGCTCCTTA 
GACTGGAACA GTTTGCTGAG ACAGAAGGCG GAATTTATTC CCCAACTGGA ATCGGAGGAT 
GACACAAGTT ATTTTGATAC TCGGTCAGAG AAGTATCATC ACATGGAGAC GGAGGAGGAG 
GACGATACAA ACGATGAGGA CTTCACCGTG GAGATAAGGC AGTTTTCTTC CTGTTCACAC 
AGGTTTTCAA AAGTTTTCAG CAGTATAGAT CGCATAACTC AAAATTCAGG AGAAGACAAA 
GATGACTCTG AGGACAAGAC CAAAAGCACA ACGTTGCCAT CCACAGAGAC ACTCAGCTGG 
AGTTCCGAAT ACTCTGAAAT GCAACAGTTA TCGACCTCCA ACTCTTCAGA TACTGAAAGC 
AACAGGTGCA AACTCAGCTC TGGCTTGCTC CCCAAGCTGG CTATTTCGAC AGATGGGGAA 
CAAGATGAGG CTGTCCCTTG CTCTGGAGAC CCCAGAGAGG AGCCAGAGAA ACCTGTCCCT 
CCCTCTGAGG AGTGTACTCA GGAGGAGCCC GAGGTCACCA CCCCAGCCAG CACCATCAGC 
AGTTCCACAC TGTCAGTTGG CAGTTTTTCA GAGCACTTGG ATCAGATAAA TGGGCGAAGC 
GAGTGTGTGG ACAGTACAGA TAATTCCTCA AAGCCATCCA GTGAACCCAC TTCTCACGTG 
GCTCGACAGC GCTTAGAAAG CACAGAGAAA AAGAAAATTT CTGGGAAAGT CACAAAGTCC 
CTCTCGGCCA GTGCTCTGTC CCTCATGATC CCAGGAGATA TGTTCGCTGT ATCTCCATTG 
GGAAGCCCAA TGTCCCCACA CTCCCTGTCT TCAGACCCTT CTTCTTCACG GGATTCCTCT 
CCCAGCCGAG ACTCTTCTGC AGCATCTGCC AGTCCGCATC AGCCCATTGT CATCCACAGC 
TCAGGCAAGA ACTATGGGTT CACCATCCGT GCTATCCGCG TGTACGTGGG GGACAGTGAC 
ATCTACACAG TGCACCATAT CGTCTGGAAC GTAGAAGAAG GAAGTCCCGC ATACCAGGCA 
GGACTGAAGG CCGGGGATCT GATCACACAC ATCAACGGAG AGCCGGTGCA CGGCCTCGTC 
CACACGGAAG TTATCGAGCT CCTGCTGAAG AGTGGGAATA AGGTGTCTAT CACCACTACT 
CCATTTGAAA ACACATCAAT CAAAACGGGA CCAGCCAGGA GAAACAGTTA CAAGGGCCGG 
ATGGTGAGAC GAAGCAAGAA GTCCAAGAAG AAGGAGAGTC TAGAAAGGAG GAGATCTCTC 
TTCAAGAAGC TGGCCAAGCA GCCTTCTCCT TTGCTCCACA CCAGCCGAAG TTTCTCCTGC 
TTAAACCGGT CCCTGTCATC TGGAGAGAGC CTCCCGGGTT CCCCAACTCA CAGCTTGTCC 
CCGAGGTCTC CAACACCCAG TTATCGTTCT ACTCCCGATT TTCCGTCAGG TACAAATTCC 
TCCCAGAGCA GCTCCCCAAG TTCAAGTGCC CCCAATTCTC CAGCAGGTTC AGGGCACATC 
CGGCCCAGCA CCCTCCATGG CCTGGCTCCC AAACTCAGCG GGCAGCGATA CCGCTCTGGA 
AGACGGAAGT CGGCTGGCAG CATCCCTCTC TCCCCGCTGG CCAGGACACC CTCTCCCACT 
CCACAGCCTA CCTCTCCTCA GCGTTCACCA TCCCCACTGT TGGGACACTC ACTGGGCAAT 
GCCAAGATCA CTCAGGCCTT TCCTAGCAAG ATGCACTCTC CCCCCACAAT CGTCAGACAC 
ATCGTGAGGC CCAAGAGTGC AGAGCCGCCC CGCTCCCCAC TGCTGAAACG GGTGCAGTCG 
GAGGAAAAGT TGTCACCCTC CTATGGCAGT GACAAGAAGC TTCTGTGCTC CCGCAAGCAT 
AGCCTAGAGG TGACACAAGA GGAGGTACAG AGGGAGCAGT GTCAGCGGGA AGTGACACTG 
CAGAGCCTGG AAGAGAATGT GTGTGACGCT CCTTCCCTCA GTCGGGCCAG GCCAGTGGAG 
CAAGGCTGTC TGAAACGCCC CGTGTCCCGG AAGGTGGGCA GGCAAGAGTC TGTGGATGAC 
CTGGACCGGG ACAAGCTGAA AGCCAAGGTT GTCGTAAAGA AACCAGAAGA GAAACATGAA 
TCGCACCAGA AACCTCACAG CCTTGGTGGT GATTCGGAAA GCTATGCTCT CTTCAGGCTA 
GAGGAGAGAG AGAAAAAAGT GTACTCCAAG GGGTTGGAAA GGTCAGGCCA TTTTGAAAAC 
ACATCAGCAG AGTTGCCTTC TGTGGGCAGC CTGCTGAAGG ACACTCTTCA CAAGCAGGCC 
AGCGTGAGGG CCAGCGAGGG GGTGACCTCA GACGGGGCAG CTTGCAGCCT GACACCAGGG 
GAGCACAGCC AGTCTCTAGG TGACTTTAAG CGGGCCTCAG CTTCTGGCAT TCTCCATGAT 
AGTGTGTGCC CCATCTCTGA TAGGCCTGCT CCTGGAAAGG TTGAATACTC GGAGAAGGCC 
TCTCAGGCCA AAGAGCTCCT TCGAAGTGAA AAACTAGACA GCAAGCTGGC CAATATTGAT 
TACCTCAGAA AGAAAATGTC ATTGGATGAC AAAGATGACA GCCACTGTGC CATCCTGAAA 
CCCAAGATAA CATCTAGCGC CCATGAATGT CTGCCAGGGA ACCCCATACG GCCCATGGCA 
GGGCAACAAG AGACCCCGCC AGCCTCTGAA AACCGGGCAT TCATCAACAG TACCCACACA 
CCTCAGATGA GTGCAGTTTC CTTTGTTCCT CTCAAAGCCT TAGCTGGCCG GGTAGAGAAC 
GGAGGGGAGA AAGCAGGCTT AGCTGCTCCC GAGTCCCCTG TCAGGAAGAG CCCCTCCGAG 
TATAAGCTAG AGGGCAGGTC AGTTTCATGT CTCAAGCCGA TCGAGGGCAC ACTGGACATT 
GCTCTCCTGT CTGGACCTCA CGCCTCCAAA ACAGAGTTGC TTTCCCCAGA GCCTGCACAG 
AGTCCCAGCC CAGGCATCAA CGTGGGACCA TGTGTGCCAC TAGCTCTTCC TGGGAGCAGT 
GGGAAAAAGG GAGACTCCAC CAGCCTGAGA GAGCCTTCCT CAGCCAACTT AAAAGTAAAT 
AAATCTTATC TGCTGGAGCC TCGGTTCCTA CCCCCGAGCC GGGCTCTCCA GGACTCTCTC 
GCAGCCTCTG GGCCAGAACC GAAGTCAAAG CCGGAAAGGA AGCTCATTCA TCCTTCTGCC 
AGGAGCCCAG CAACTGTCAC AGAGAGCAAT CTTCAGCAGA AAGAGGGTGG TCCCGCCACA 
CACCAAGACC GCTCCACTGA CACCAGGAAC CTCCCTGGCC CAGGGCAGAC CCTACACAAT 
GTGGACCTAC CCAGGCTGTG TACACGTGCC CCACTCCCAC CGGAAGGGAC GCCCGCAAAG 
GAGAAGCCAT GTCTGAAGGA ACCCTCTGCC AAGGTGAAAA GCGAGTGGTC TGCCGTGAGG 
GATGACGGAC ACAGAGATCC CTGTGCGAAG CTGTGCCCGG CAGAGACTGG TAAAGCCAGC 
GACAGTTCCA AACCCCTGCC TTCCGGGGGG AGGACCCAAC CCGATTTCTA CAAGCAGACC 
CAGACTTCGG AGAAAGCATG GGCGCATGCA AAAACAAACC ACAAAGATAG CCAAGATGAG 
GTGAAGTCCC TGGCCAGGGA GGACTCAGCT TCACTTTTAT ATGAAAAGGA GATAGGCCGG 
GCACGAAAAG GTCCTGAACC CAAACCGGAA GTTCCTGCTA CCCGGTGCCC TCCTCAGCCA 
CCAGGAATTG AGGGTGAGAA GCGAGAAAAG CTCTCCGCTG CCCCCTCTTT GCAGAAACAG 
GCTCCCAAAG AGCCAGACAG GAAGGAACAG ACTTCGCAAA GGCCTGGAGG TAGTGGCCCT 
CAACAACCCC CACCCACCAA AGAGCTGTCT AACTCAGCAT CCTGGCAGCA CGGCAGTTCT 
CCGAGTCACA CTTTAAAGAA GGAGCCCGGG ACCAAAGCTG CCGCTGCAGA ACCAAGCACC 
AGCCTTCATG ACACTCCCCG ATCTGCTACA GCCACCACCA CTGCCATTGC CACCACCACC 
ACTACCACCA GTGCCGGGCA CAGTGACTGC AGTAGCCATA AGGCCCGGCC TGGCCCTGAC 
CCCAGCCCTT CAAAGTCTAA GCACCAAGAC AGGTCCCTCT CCTCACAGAA GCTGAGTGCT 
GGCTCTGCAA AAGGCAAAGA GCCTGTCACT CAACCCCTGG GTGGTTCCAT CAGAGAAGGC 
AAGGGTGGCA GCAAGGGTCC AGTGGACACA TTTTCTGCTG TCCTGACCAC CCAGGGCAAA 
GCAAGTGATG TGCTTGTGCA GGGAGAAGGT CGGGTCTCAA TCATTGTCCA CACTGAAGAG 
TGTCCTCTCG ATGCCAAACT GAAAAACACC AATGGAGGGT GTCCCCCAGA GATGCAGGCG 
AAGCATCCAC CCAGACAAGG ACATCTCAGT GAAGCAGCAG ACCAGAAGCC ACTCATTGCT 
GGTGAGAAGC AAAGCCCGTC TCCAAAGCAT CCCAAACCAT CCACTGTGAA AGATTACCCC 
AGTCTGTGCA GACAGACAGA CAGAAGCCCA AGCCATCAGG CTACCACTGG GGACAGGAAG 
GCAGAAGGAA AGAAATGCAC AGACGCACTT TATGTCGCAG CCCCAGAGGG CTACAAGCCA 
GAGGCCAGCC CTTCTCTCCA CCACGGCGAG ACCGGACTCA GAGGCTCAGA GAGGCCACCC 
ATGGGCATGG GGAAGGGCTT CTCTGAGCCC AAGGGGAAAG GGCCAGGTCC CCAGAAGTCA 
CTGGCTGAAA CAGGCAAGCC CAGCGGTATG AAAAGGTCAC CCTCTGCCAC CGTGCAGAGC 
TCTCTCCGCT CAGCTGCCCC CCCAGAAAAG TCTCTGAGTT ACTCAGCCAG CTTTCCCGAG 
GCCCAGCCTG GAGTGCGAGA GGTCCCTGCA GCCAACAGCA GCCCCTCATC TGCCAAGGCT 
ACAGGGGGGA CCTCAGAGTT CCCAGCCCCC AGCAGCAGGG ACCACAGGAA GCTTCAGTCT 
GGAGGAGACG GCCGAAGCCA AATGATAAAG AGTGACTCTC TGCCCTCCTT CCGCCTCTCC 
ACCTCTGCTC TGGAGTCACA TTTCCAGGAT CCACAGGTGC CCATCGCATC AGGCCACCGA 
GGCAGGGCAC TGTCAGTAAC TGCTGCCACA GGAGAACCCA AAGGGAGAGA GCTCGCCCAG 
CCTCCCCCAG TCAGGAAACA GAATGCGTGC AGAGAGGCGA CCAGAGCACC CCCAGCCCCA 
AGCACAGATC GCTCCCTCCC TCTTTCCTCA GAGAAAGACT TCGTGGTTCG GCAGAGAAGG 
GGCAAGGAGA CCTTAAGGAG CAGTCCTCAC AAAAAGGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517765.1
CDS	106..7410
Translation	MDESSILRRRGLQKELSLPRRGSLVDSQKWNCLVKRCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_006517702.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517702.4

ORF Insert Sequence:

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGA AGGAGCTGAG CCTCCCCCGA 
AGAGGAAGTC TGGTAGATTC CCAGAAGTGG AATTGCTTGG TCAAACGTTG CCGAACAAGC 
AACCGGAAGA GTTTGATAGG CAATGGCCAG TCTCCAGCAC TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GACAGTCCAA GGAATTTCTC CCCCAGTGCC 
TCAGCCCATT TCTCATTTGC GAGGAGAAAT GACAGAACGG ATGGGCGCCG CTGGTCTCTG 
GCTTCTCTCC CTTCCTCAGG CTATGGGACA AATACCCCAA GCTCCACCGT CTCCTCATCC 
TGTTCTTCCC AGGAGAAGTT GCACCAGCTA CCATACCAGC CAACCCCAGA TGAATTACAC 
TTCTTATCCA AACACTTCTG CACAACAGAA AGTATCGCCA CTGAGAACCG GTGCAGAAAC 
ACACCCATGC GTCCACGTTC CCGGAGTCTC AGCCCTGGAC GGTCCCCCGC CTGCTGTGAC 
CATGAAATAA TTATGATGAA CCATGTCTAC AAAGAAAGGT TCCCAAAGGC CACAGCTCAG 
ATGGAAGAGC GTCTGAAGGA GATCATCACC AGCTACTCTC CAGACCATGT TCTCCCCTTG 
GCAGATGGGG TACTTAGTTT CACTCACCAT CAGATCATTG AGCTGGCTCG GGACTGTTTG 
GATAAATCTC ACCAGGGTCT CATCACGTCG AGATACTTTT TTGAGTTGCA GCACAAACTG 
GACAAGTTGC TCCAGGAGGC TCACGATCGC TCTGAAAGTG GAGAACTGGC ATTTATCAAG 
CAACTAGTCC GAAAGATCCT AATTGTCATT GCCCGCCCCG CTCGGTTATT GGAGTGTTTG 
GAATTTGATC CTGAAGAATT TTATTACCTA TTGGAAGCTG CAGAAGGCCA TGCCAAAGAA 
GGCCAAGGAA TCAAAACTGA CATCCCTAGG TATATCATCA GCCAGCTGGG ACTCAATAAG 
GACCCTTTAG AAGAAATGGC TCAGTTGGGG AATTACGACA GTAGGACAGC AGAGACACCA 
GAGATGGATG AGTCAGTGAG TAGCTCAAAT ACTTCCCTGA GACTTCGAAG GAAACCCCGA 
GAGAGTGATT TTGAAACAAT TAAATTGATC AGCAATGGAG CCTACGGGGC AGTCTACTTT 
GTTCGGCACA AAGAGTCCCG CCAGAGGTTT GCCATGAAGA AGATCAACAA GCAGAACCTC 
ATCCTTCGGA ACCAGATCCA GCAGGCCTTC GTGGAGCGAG ACATCCTGAC TTTCGCAGAG 
AACCCCTTTG TGGTCAGCAT GTATTGCTCC TTTGAAACGA GGCGTCACTT ATGCATGGTC 
ATGGAGTATG TAGAAGGGGG AGACTGTGCG ACCCTAATGA AAAACATGGG ACCTCTCCCA 
GTTGATATGG CCAGAATGTA TTTCGCCGAG ACCGTCTTGG CCTTGGAGTA CCTGCATAAT 
TACGGAATCG TACACAGGGA CTTGAAGCCA GACAACCTGT TGGTCACCTC CATGGGGCAC 
ATAAAACTGA CTGACTTCGG CTTGTCTAAG GTGGGATTAA TGAGCATGAC CACCAATCTC 
TATGAAGGCC ACATAGAGAA GGACGCTCGA GAGTTCTTAG ATAAACAGGT CTGTGGTACA 
CCTGAGTACA TTGCCCCCGA GGTGATTCTG AGACAGGGCT ACGGGAAACC CGTGGACTGG 
TGGGCCATGG GCATTATCCT CTATGAATTT CTGGTCGGAT GTGTGCCTTT CTTTGGGGAC 
ACTCCAGAAG AGCTATTTGG ACAAGTCATC AGTGATGAAA TCAACTGGCC TGAAAAGGAC 
GAGGCCCCTC CTCCAGACGC TCAGGAGCTG ATTACCTTGC TCCTCAGGCA GAATCCGCTG 
GAGAGGCTGG GAACAGGTGG AGCCTATGAA GTGAAGCAGC ATCGTTTCTT CCGCTCCTTA 
GACTGGAACA GTTTGCTGAG ACAGAAGGCG GAATTTATTC CCCAACTGGA ATCGGAGGAT 
GACACAAGTT ATTTTGATAC TCGGTCAGAG AAGTATCATC ACATGGAGAC GGAGGAGGAG 
GACGATACAA ACGATGAGGA CTTCACCGTG GAGATAAGGC AGTTTTCTTC CTGTTCACAC 
AGGTTTTCAA AAGTTTTCAG CAGTATAGAT CGCATAACTC AAAATTCAGG AGAAGACAAA 
GATGACTCTG AGGACAAGAC CAAAAGCACA ACGTTGCCAT CCACAGAGAC ACTCAGCTGG 
AGTTCCGAAT ACTCTGAAAT GCAACAGTTA TCGACCTCCA ACTCTTCAGA TACTGAAAGC 
AACAGGTGCA AACTCAGCTC TGGCTTGCTC CCCAAGCTGG CTATTTCGAC AGATGGGGAA 
CAAGATGAGG CTGTCCCTTG CTCTGGAGAC CCCAGAGAGG AGCCAGAGAA ACCTGTCCCT 
CCCTCTGAGG AGTGTACTCA GGAGGAGCCC GAGGTCACCA CCCCAGCCAG CACCATCAGC 
AGTTCCACAC TGTCAGTTGG CAGTTTTTCA GAGCACTTGG ATCAGATAAA TGGGCGAAGC 
GAGTGTGTGG ACAGTACAGA TAATTCCTCA AAGCCATCCA GTGAACCCAC TTCTCACGTG 
GCTCGACAGC GCTTAGAAAG CACAGAGAAA AAGAAAATTT CTGGGAAAGT CACAAAGTCC 
CTCTCGGCCA GTGCTCTGTC CCTCATGATC CCAGGAGATA TGTTCGCTGT ATCTCCATTG 
GGAAGCCCAA TGTCCCCACA CTCCCTGTCT TCAGACCCTT CTTCTTCACG GGATTCCTCT 
CCCAGCCGAG ACTCTTCTGC AGCATCTGCC AGTCCGCATC AGCCCATTGT CATCCACAGC 
TCAGGCAAGA ACTATGGGTT CACCATCCGT GCTATCCGCG TGTACGTGGG GGACAGTGAC 
ATCTACACAG TGCACCATAT CGTCTGGAAC GTAGAAGAAG GAAGTCCCGC ATACCAGGCA 
GGACTGAAGG CCGGGGATCT GATCACACAC ATCAACGGAG AGCCGGTGCA CGGCCTCGTC 
CACACGGAAG TTATCGAGCT CCTGCTGAAG AGTGGGAATA AGGTGTCTAT CACCACTACT 
CCATTTGAAA ACACATCAAT CAAAACGGGA CCAGCCAGGA GAAACAGTTA CAAGGGCCGG 
ATGGTGAGAC GAAGCAAGAA GTCCAAGAAG AAGGAGAGTC TAGAAAGGAG GAGATCTCTC 
TTCAAGAAGC TGGCCAAGCA GCCTTCTCCT TTGCTCCACA CCAGCCGAAG TTTCTCCTGC 
TTAAACCGGT CCCTGTCATC TGGAGAGAGC CTCCCGGGTT CCCCAACTCA CAGCTTGTCC 
CCGAGGTCTC CAACACCCAG TTATCGTTCT ACTCCCGATT TTCCGTCAGG TACAAATTCC 
TCCCAGAGCA GCTCCCCAAG TTCAAGTGCC CCCAATTCTC CAGCAGGTTC AGGGCACATC 
CGGCCCAGCA CCCTCCATGG CCTGGCTCCC AAACTCAGCG GGCAGCGATA CCGCTCTGGA 
AGACGGAAGT CGGCTGGCAG CATCCCTCTC TCCCCGCTGG CCAGGACACC CTCTCCCACT 
CCACAGCCTA CCTCTCCTCA GCGTTCACCA TCCCCACTGT TGGGACACTC ACTGGGCAAT 
GCCAAGATCA CTCAGGCCTT TCCTAGCAAG ATGCACTCTC CCCCCACAAT CGTCAGACAC 
ATCGTGAGGC CCAAGAGTGC AGAGCCGCCC CGCTCCCCAC TGCTGAAACG GGTGCAGTCG 
GAGGAAAAGT TGTCACCCTC CTATGGCAGT GACAAGAAGC TTCTGTGCTC CCGCAAGCAT 
AGCCTAGAGG TGACACAAGA GGAGGTACAG AGGGAGCAGT GTCAGCGGGA AGTGACACTG 
CAGAGCCTGG AAGAGAATGT GTGTGACGCT CCTTCCCTCA GTCGGGCCAG GCCAGTGGAG 
CAAGGCTGTC TGAAACGCCC CGTGTCCCGG AAGGTGGGCA GGCAAGAGTC TGTGGATGAC 
CTGGACCGGG ACAAGCTGAA AGCCAAGGTT GTCGTAAAGA AACCAGAAGA GAAACATGAA 
TCGCACCAGA AACCTCACAG CCTTGGTGGT GATTCGGAAA GCTATGCTCT CTTCAGGCTA 
GAGGAGAGAG AGAAAAAAGT GTACTCCAAG GGGTTGGAAA GGTCAGGCCA TTTTGAAAAC 
ACATCAGCAG AGTTGCCTTC TGTGGGCAGC CTGCTGAAGG ACACTCTTCA CAAGCAGGCC 
AGCGTGAGGG CCAGCGAGGG GGTGACCTCA GACGGGGCAG CTTGCAGCCT GACACCAGGG 
GAGCACAGCC AGTCTCTAGG TGACTTTAAG CGGGCCTCAG CTTCTGGCAT TCTCCATGAT 
AGTGTGTGCC CCATCTCTGA TAGGCCTGCT CCTGGAAAGG TTGAATACTC GGAGAAGGCC 
TCTCAGGCCA AAGAGCTCCT TCGAAGTGAA AAACTAGACA GCAAGCTGGC CAATATTGAT 
TACCTCAGAA AGAAAATGTC ATTGGATGAC AAAGATGACA GCCACTGTGC CATCCTGAAA 
CCCAAGATAA CATCTAGCGC CCATGAATGT CTGCCAGGGA ACCCCATACG GCCCATGGCA 
GGGCAACAAG AGACCCCGCC AGCCTCTGAA AACCGGGCAT TCATCAACAG TACCCACACA 
CCTCAGATGA GTGCAGTTTC CTTTGTTCCT CTCAAAGCCT TAGCTGGCCG GGTAGAGAAC 
GGAGGGGAGA AAGCAGGCTT AGCTGCTCCC GAGTCCCCTG TCAGGAAGAG CCCCTCCGAG 
TATAAGCTAG AGGGCAGGTC AGTTTCATGT CTCAAGCCGA TCGAGGGCAC ACTGGACATT 
GCTCTCCTGT CTGGACCTCA CGCCTCCAAA ACAGAGTTGC TTTCCCCAGA GCCTGCACAG 
AGTCCCAGCC CAGGCATCAA CGTGGGACCA TGTGTGCCAC TAGCTCTTCC TGGGAGCAGT 
GGGAAAAAGG GAGACTCCAC CAGCCTGAGA GAGCCTTCCT CAGCCAACTT AAAAGTAAAT 
AAATCTTATC TGCTGGAGCC TCGGTTCCTA CCCCCGAGCC GGGCTCTCCA GGACTCTCTC 
GCAGCCTCTG GGCCAGAACC GAAGTCAAAG CCGGAAAGGA AGCTCATTCA TCCTTCTGCC 
AGGAGCCCAG CAACTGTCAC AGAGAGCAAT CTTCAGCAGA AAGAGGGTGG TCCCGCCACA 
CACCAAGACC GCTCCACTGA CACCAGGAAC CTCCCTGGCC CAGGGCAGAC CCTACACAAT 
GTGGACCTAC CCAGGCTGTG TACACGTGCC CCACTCCCAC CGGAAGGGAC GCCCGCAAAG 
GAGAAGCCAT GTCTGAAGGA ACCCTCTGCC AAGGTGAAAA GCGAGTGGTC TGCCGTGAGG 
GATGACGGAC ACAGAGATCC CTGTGCGAAG CTGTGCCCGG CAGAGACTGG TAAAGCCAGC 
GACAGTTCCA AACCCCTGCC TTCCGGGGGG AGGACCCAAC CCGATTTCTA CAAGCAGACC 
CAGACTTCGG AGAAAGCATG GGCGCATGCA AAAACAAACC ACAAAGATAG CCAAGATGAG 
GTGAAGTCCC TGGCCAGGGA GGACTCAGCT TCACTTTTAT ATGAAAAGGA GATAGGCCGG 
GCACGAAAAG GTCCTGAACC CAAACCGGAA GTTCCTGCTA CCCGGTGCCC TCCTCAGCCA 
CCAGGAATTG AGGGTGAGAA GCGAGAAAAG CTCTCCGCTG CCCCCTCTTT GCAGAAACAG 
GCTCCCAAAG AGCCAGACAG GAAGGAACAG ACTTCGCAAA GGCCTGGAGG TAGTGGCCCT 
CAACAACCCC CACCCACCAA AGAGCTGTCT AACTCAGCAT CCTGGCAGCA CGGCAGTTCT 
CCGAGTCACA CTTTAAAGAA GGAGCCCGGG ACCAAAGCTG CCGCTGCAGA ACCAAGCACC 
AGCCTTCATG ACACTCCCCG ATCTGCTACA GCCACCACCA CTGCCATTGC CACCACCACC 
ACTACCACCA GTGCCGGGCA CAGTGACTGC AGTAGCCATA AGGCCCGGCC TGGCCCTGAC 
CCCAGCCCTT CAAAGTCTAA GCACCAAGAC AGGTCCCTCT CCTCACAGAA GCTGAGTGCT 
GGCTCTGCAA AAGGCAAAGA GCCTGTCACT CAACCCCTGG GTGGTTCCAT CAGAGAAGGC 
AAGGGTGGCA GCAAGGGTCC AGTGGACACA TTTTCTGCTG TCCTGACCAC CCAGGGCAAA 
GCAAGTGATG TGCTTGTGCA GGGAGAAGGT CGGGTCTCAA TCATTGTCCA CACTGAAGAG 
TGTCCTCTCG ATGCCAAACT GAAAAACACC AATGGAGGGT GTCCCCCAGA GATGCAGGCG 
AAGCATCCAC CCAGACAAGG ACATCTCAGT GAAGCAGCAG ACCAGAAGCC ACTCATTGCT 
GGTGAGAAGC AAAGCCCGTC TCCAAAGCAT CCCAAACCAT CCACTGTGAA AGATTACCCC 
AGTCTGTGCA GACAGACAGA CAGAAGCCCA AGCCATCAGG CTACCACTGG GGACAGGAAG 
GCAGAAGGAA AGAAATGCAC AGACGCACTT TATGTCGCAG CCCCAGAGGG CTACAAGCCA 
GAGGCCAGCC CTTCTCTCCA CCACGGCGAG ACCGGACTCA GAGGCTCAGA GAGGCCACCC 
ATGGGCATGG GGAAGGGCTT CTCTGAGCCC AAGGGGAAAG GGCCAGGTCC CCAGAAGTCA 
CTGGCTGAAA CAGGCAAGCC CAGCGGTATG AAAAGGTCAC CCTCTGCCAC CGTGCAGAGC 
TCTCTCCGCT CAGCTGCCCC CCCAGAAAAG TCTCTGAGTT ACTCAGCCAG CTTTCCCGAG 
GCCCAGCCTG GAGTGCGAGA GGTCCCTGCA GCCAACAGCA GCCCCTCATC TGCCAAGGCT 
ACAGGGGGGA CCTCAGAGTT CCCAGCCCCC AGCAGCAGGG ACCACAGGAA GCTTCAGTCT 
GGAGGAGACG GCCGAAGCCA AATGATAAAG AGTGACTCTC TGCCCTCCTT CCGCCTCTCC 
ACCTCTGCTC TGGAGTCACA TTTCCAGGAT CCACAGGTGC CCATCGCATC AGGCCACCGA 
GGCAGGGCAC TGTCAGTAAC TGCTGCCACA GGAGAACCCA AAGGGAGAGA GCTCGCCCAG 
CCTCCCCCAG TCAGGAAACA GAATGCGTGC AGAGAGGCGA CCAGAGCACC CCCAGCCCCA 
AGCACAGATC GCTCCCTCCC TCTTTCCTCA GAGAAAGACT TCGTGGTTCG GCAGAGAAGG 
GGCAAGGAGA CCTTAAGGAG CAGTCCTCAC AAAAAGGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_006517703.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517766.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X13, mRNA.

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CloneID	OMu49079
Clone ID Related Accession (Same CDS sequence)	XM_006517703.4 , XM_006517703.3
Accession Version	XM_006517703.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517703.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATGT CTGACCCCAA TTTCTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGCACACAA AGTTGCCGAA CAAGCAACCG GAAGAGTTTG 
ATAGGCAATG GCCAGTCTCC AGCACTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGACAG TCCAAGGAAT TTCTCCCCCA GTGCCTCAGC CCATTTCTCA 
TTTGCGAGGA GAAATGACAG AACGGATGGG CGCCGCTGGT CTCTGGCTTC TCTCCCTTCC 
TCAGGCTATG GGACAAATAC CCCAAGCTCC ACCGTCTCCT CATCCTGTTC TTCCCAGGAG 
AAGTTGCACC AGCTACCATA CCAGCCAACC CCAGATGAAT TACACTTCTT ATCCAAACAC 
TTCTGCACAA CAGAAAGTAT CGCCACTGAG AACCGGTGCA GAAACACACC CATGCGTCCA 
CGTTCCCGGA GTCTCAGCCC TGGACGGTCC CCCGCCTGCT GTGACCATGA AATAATTATG 
ATGAACCATG TCTACAAAGA AAGGTTCCCA AAGGCCACAG CTCAGATGGA AGAGCGTCTG 
AAGGAGATCA TCACCAGCTA CTCTCCAGAC CATGTTCTCC CCTTGGCAGA TGGGGTACTT 
AGTTTCACTC ACCATCAGAT CATTGAGCTG GCTCGGGACT GTTTGGATAA ATCTCACCAG 
GGTCTCATCA CGTCGAGATA CTTTTTTGAG TTGCAGCACA AACTGGACAA GTTGCTCCAG 
GAGGCTCACG ATCGCTCTGA AAGTGGAGAA CTGGCATTTA TCAAGCAACT AGTCCGAAAG 
ATCCTAATTG TCATTGCCCG CCCCGCTCGG TTATTGGAGT GTTTGGAATT TGATCCTGAA 
GAATTTTATT ACCTATTGGA AGCTGCAGAA GGCCATGCCA AAGAAGGCCA AGGAATCAAA 
ACTGACATCC CTAGGTATAT CATCAGCCAG CTGGGACTCA ATAAGGACCC TTTAGAAGAA 
ATGGCTCAGT TGGGGAATTA CGACAGTAGG ACAGCAGAGA CACCAGAGAT GGATGAGTCA 
GTGAGTAGCT CAAATACTTC CCTGAGACTT CGAAGGAAAC CCCGAGAGAG TGATTTTGAA 
ACAATTAAAT TGATCAGCAA TGGAGCCTAC GGGGCAGTCT ACTTTGTTCG GCACAAAGAG 
TCCCGCCAGA GGTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTCATCCT TCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGAGACATC CTGACTTTCG CAGAGAACCC CTTTGTGGTC 
AGCATGTATT GCTCCTTTGA AACGAGGCGT CACTTATGCA TGGTCATGGA GTATGTAGAA 
GGGGGAGACT GTGCGACCCT AATGAAAAAC ATGGGACCTC TCCCAGTTGA TATGGCCAGA 
ATGTATTTCG CCGAGACCGT CTTGGCCTTG GAGTACCTGC ATAATTACGG AATCGTACAC 
AGGGACTTGA AGCCAGACAA CCTGTTGGTC ACCTCCATGG GGCACATAAA ACTGACTGAC 
TTCGGCTTGT CTAAGGTGGG ATTAATGAGC ATGACCACCA ATCTCTATGA AGGCCACATA 
GAGAAGGACG CTCGAGAGTT CTTAGATAAA CAGGTCTGTG GTACACCTGA GTACATTGCC 
CCCGAGGTGA TTCTGAGACA GGGCTACGGG AAACCCGTGG ACTGGTGGGC CATGGGCATT 
ATCCTCTATG AATTTCTGGT CGGATGTGTG CCTTTCTTTG GGGACACTCC AGAAGAGCTA 
TTTGGACAAG TCATCAGTGA TGAAATCAAC TGGCCTGAAA AGGACGAGGC CCCTCCTCCA 
GACGCTCAGG AGCTGATTAC CTTGCTCCTC AGGCAGAATC CGCTGGAGAG GCTGGGAACA 
GGTGGAGCCT ATGAAGTGAA GCAGCATCGT TTCTTCCGCT CCTTAGACTG GAACAGTTTG 
CTGAGACAGA AGGCGGAATT TATTCCCCAA CTGGAATCGG AGGATGACAC AAGTTATTTT 
GATACTCGGT CAGAGAAGTA TCATCACATG GAGACGGAGG AGGAGGACGA TACAAACGAT 
GAGGACTTCA CCGTGGAGAT AAGGCAGTTT TCTTCCTGTT CACACAGGTT TTCAAAAGTT 
TTCAGCAGTA TAGATCGCAT AACTCAAAAT TCAGGAGAAG ACAAAGATGA CTCTGAGGAC 
AAGACCAAAA GCACAACGTT GCCATCCACA GAGACACTCA GCTGGAGTTC CGAATACTCT 
GAAATGCAAC AGTTATCGAC CTCCAACTCT TCAGATACTG AAAGCAACAG GTGCAAACTC 
AGCTCTGGCT TGCTCCCCAA GCTGGCTATT TCGACAGATG GGGAACAAGA TGAGGCTGTC 
CCTTGCTCTG GAGACCCCAG AGAGGAGCCA GAGAAACCTG TCCCTCCCTC TGAGGAGTGT 
ACTCAGGAGG AGCCCGAGGT CACCACCCCA GCCAGCACCA TCAGCAGTTC CACACTGTCA 
GTTGGCAGTT TTTCAGAGCA CTTGGATCAG ATAAATGGGC GAAGCGAGTG TGTGGACAGT 
ACAGATAATT CCTCAAAGCC ATCCAGTGAA CCCACTTCTC ACGTGGCTCG ACAGCGCTTA 
GAAAGCACAG AGAAAAAGAA AATTTCTGGG AAAGTCACAA AGTCCCTCTC GGCCAGTGCT 
CTGTCCCTCA TGATCCCAGG AGATATGTTC GCTGTATCTC CATTGGGAAG CCCAATGTCC 
CCACACTCCC TGTCTTCAGA CCCTTCTTCT TCACGGGATT CCTCTCCCAG CCGAGACTCT 
TCTGCAGCAT CTGCCAGTCC GCATCAGCCC ATTGTCATCC ACAGCTCAGG CAAGAACTAT 
GGGTTCACCA TCCGTGCTAT CCGCGTGTAC GTGGGGGACA GTGACATCTA CACAGTGCAC 
CATATCGTCT GGAACGTAGA AGAAGGAAGT CCCGCATACC AGGCAGGACT GAAGGCCGGG 
GATCTGATCA CACACATCAA CGGAGAGCCG GTGCACGGCC TCGTCCACAC GGAAGTTATC 
GAGCTCCTGC TGAAGAGTGG GAATAAGGTG TCTATCACCA CTACTCCATT TGAAAACACA 
TCAATCAAAA CGGGACCAGC CAGGAGAAAC AGTTACAAGG GCCGGATGGT GAGACGAAGC 
AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC 
AAGCAGCCTT CTCCTTTGCT CCACACCAGC CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG 
TCATCTGGAG AGAGCCTCCC GGGTTCCCCA ACTCACAGCT TGTCCCCGAG GTCTCCAACA 
CCCAGTTATC GTTCTACTCC CGATTTTCCG TCAGGTACAA ATTCCTCCCA GAGCAGCTCC 
CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA GGTTCAGGGC ACATCCGGCC CAGCACCCTC 
CATGGCCTGG CTCCCAAACT CAGCGGGCAG CGATACCGCT CTGGAAGACG GAAGTCGGCT 
GGCAGCATCC CTCTCTCCCC GCTGGCCAGG ACACCCTCTC CCACTCCACA GCCTACCTCT 
CCTCAGCGTT CACCATCCCC ACTGTTGGGA CACTCACTGG GCAATGCCAA GATCACTCAG 
GCCTTTCCTA GCAAGATGCA CTCTCCCCCC ACAATCGTCA GACACATCGT GAGGCCCAAG 
AGTGCAGAGC CGCCCCGCTC CCCACTGCTG AAACGGGTGC AGTCGGAGGA AAAGTTGTCA 
CCCTCCTATG GCAGTGACAA GAAGCTTCTG TGCTCCCGCA AGCATAGCCT AGAGGTGACA 
CAAGAGGAGG TACAGAGGGA GCAGTGTCAG CGGGAAGTGA CACTGCAGAG CCTGGAAGAG 
AATGTGTGTG ACGCTCCTTC CCTCAGTCGG GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA 
CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA GAGTCTGTGG ATGACCTGGA CCGGGACAAG 
CTGAAAGCCA AGGTTGTCGT AAAGAAACCA GAAGAGAAAC ATGAATCGCA CCAGAAACCT 
CACAGCCTTG GTGGTGATTC GGAAAGCTAT GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA 
AAAGTGTACT CCAAGGGGTT GGAAAGGTCA GGCCATTTTG AAAACACATC AGCAGAGTTG 
CCTTCTGTGG GCAGCCTGCT GAAGGACACT CTTCACAAGC AGGCCAGCGT GAGGGCCAGC 
GAGGGGGTGA CCTCAGACGG GGCAGCTTGC AGCCTGACAC CAGGGGAGCA CAGCCAGTCT 
CTAGGTGACT TTAAGCGGGC CTCAGCTTCT GGCATTCTCC ATGATAGTGT GTGCCCCATC 
TCTGATAGGC CTGCTCCTGG AAAGGTTGAA TACTCGGAGA AGGCCTCTCA GGCCAAAGAG 
CTCCTTCGAA GTGAAAAACT AGACAGCAAG CTGGCCAATA TTGATTACCT CAGAAAGAAA 
ATGTCATTGG ATGACAAAGA TGACAGCCAC TGTGCCATCC TGAAACCCAA GATAACATCT 
AGCGCCCATG AATGTCTGCC AGGGAACCCC ATACGGCCCA TGGCAGGGCA ACAAGAGACC 
CCGCCAGCCT CTGAAAACCG GGCATTCATC AACAGTACCC ACACACCTCA GATGAGTGCA 
GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT GGCCGGGTAG AGAACGGAGG GGAGAAAGCA 
GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG AAGAGCCCCT CCGAGTATAA GCTAGAGGGC 
AGGTCAGTTT CATGTCTCAA GCCGATCGAG GGCACACTGG ACATTGCTCT CCTGTCTGGA 
CCTCACGCCT CCAAAACAGA GTTGCTTTCC CCAGAGCCTG CACAGAGTCC CAGCCCAGGC 
ATCAACGTGG GACCATGTGT GCCACTAGCT CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC 
TCCACCAGCC TGAGAGAGCC TTCCTCAGCC AACTTAAAAG TAAATAAATC TTATCTGCTG 
GAGCCTCGGT TCCTACCCCC GAGCCGGGCT CTCCAGGACT CTCTCGCAGC CTCTGGGCCA 
GAACCGAAGT CAAAGCCGGA AAGGAAGCTC ATTCATCCTT CTGCCAGGAG CCCAGCAACT 
GTCACAGAGA GCAATCTTCA GCAGAAAGAG GGTGGTCCCG CCACACACCA AGACCGCTCC 
ACTGACACCA GGAACCTCCC TGGCCCAGGG CAGACCCTAC ACAATGTGGA CCTACCCAGG 
CTGTGTACAC GTGCCCCACT CCCACCGGAA GGGACGCCCG CAAAGGAGAA GCCATGTCTG 
AAGGAACCCT CTGCCAAGGT GAAAAGCGAG TGGTCTGCCG TGAGGGATGA CGGACACAGA 
GATCCCTGTG CGAAGCTGTG CCCGGCAGAG ACTGGTAAAG CCAGCGACAG TTCCAAACCC 
CTGCCTTCCG GGGGGAGGAC CCAACCCGAT TTCTACAAGC AGACCCAGAC TTCGGAGAAA 
GCATGGGCGC ATGCAAAAAC AAACCACAAA GATAGCCAAG ATGAGGTGAA GTCCCTGGCC 
AGGGAGGACT CAGCTTCACT TTTATATGAA AAGGAGATAG GCCGGGCACG AAAAGGTCCT 
GAACCCAAAC CGGAAGTTCC TGCTACCCGG TGCCCTCCTC AGCCACCAGG AATTGAGGGT 
GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC TCTTTGCAGA AACAGGCTCC CAAAGAGCCA 
GACAGGAAGG AACAGACTTC GCAAAGGCCT GGAGGTAGTG GCCCTCAACA ACCCCCACCC 
ACCAAAGAGC TGTCTAACTC AGCATCCTGG CAGCACGGCA GTTCTCCGAG TCACACTTTA 
AAGAAGGAGC CCGGGACCAA AGCTGCCGCT GCAGAACCAA GCACCAGCCT TCATGACACT 
CCCCGATCTG CTACAGCCAC CACCACTGCC ATTGCCACCA CCACCACTAC CACCAGTGCC 
GGGCACAGTG ACTGCAGTAG CCATAAGGCC CGGCCTGGCC CTGACCCCAG CCCTTCAAAG 
TCTAAGCACC AAGACAGGTC CCTCTCCTCA CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC 
AAAGAGCCTG TCACTCAACC CCTGGGTGGT TCCATCAGAG AAGGCAAGGG TGGCAGCAAG 
GGTCCAGTGG ACACATTTTC TGCTGTCCTG ACCACCCAGG GCAAAGCAAG TGATGTGCTT 
GTGCAGGGAG AAGGTCGGGT CTCAATCATT GTCCACACTG AAGAGTGTCC TCTCGATGCC 
AAACTGAAAA ACACCAATGG AGGGTGTCCC CCAGAGATGC AGGCGAAGCA TCCACCCAGA 
CAAGGACATC TCAGTGAAGC AGCAGACCAG AAGCCACTCA TTGCTGGTGA GAAGCAAAGC 
CCGTCTCCAA AGCATCCCAA ACCATCCACT GTGAAAGATT ACCCCAGTCT GTGCAGACAG 
ACAGACAGAA GCCCAAGCCA TCAGGCTACC ACTGGGGACA GGAAGGCAGA AGGAAAGAAA 
TGCACAGACG CACTTTATGT CGCAGCCCCA GAGGGCTACA AGCCAGAGGC CAGCCCTTCT 
CTCCACCACG GCGAGACCGG ACTCAGAGGC TCAGAGAGGC CACCCATGGG CATGGGGAAG 
GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA GGTCCCCAGA AGTCACTGGC TGAAACAGGC 
AAGCCCAGCG GTATGAAAAG GTCACCCTCT GCCACCGTGC AGAGCTCTCT CCGCTCAGCT 
GCCCCCCCAG AAAAGTCTCT GAGTTACTCA GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG 
CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC TCATCTGCCA AGGCTACAGG GGGGACCTCA 
GAGTTCCCAG CCCCCAGCAG CAGGGACCAC AGGAAGCTTC AGTCTGGAGG AGACGGCCGA 
AGCCAAATGA TAAAGAGTGA CTCTCTGCCC TCCTTCCGCC TCTCCACCTC TGCTCTGGAG 
TCACATTTCC AGGATCCACA GGTGCCCATC GCATCAGGCC ACCGAGGCAG GGCACTGTCA 
GTAACTGCTG CCACAGGAGA ACCCAAAGGG AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG 
AAACAGAATG CGTGCAGAGA GGCGACCAGA GCACCCCCAG CCCCAAGCAC AGATCGCTCC 
CTCCCTCTTT CCTCAGAGAA AGACTTCGTG GTTCGGCAGA GAAGGGGCAA GGAGACCTTA 
AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517766.1
CDS	1238..8527
Translation	MDMSDPNFWTVLSNFTLPHLRSGNRLRRTQSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_006517703.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517703.4

ORF Insert Sequence:

ATGGATATGT CTGACCCCAA TTTCTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGCACACAA AGTTGCCGAA CAAGCAACCG GAAGAGTTTG 
ATAGGCAATG GCCAGTCTCC AGCACTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGACAG TCCAAGGAAT TTCTCCCCCA GTGCCTCAGC CCATTTCTCA 
TTTGCGAGGA GAAATGACAG AACGGATGGG CGCCGCTGGT CTCTGGCTTC TCTCCCTTCC 
TCAGGCTATG GGACAAATAC CCCAAGCTCC ACCGTCTCCT CATCCTGTTC TTCCCAGGAG 
AAGTTGCACC AGCTACCATA CCAGCCAACC CCAGATGAAT TACACTTCTT ATCCAAACAC 
TTCTGCACAA CAGAAAGTAT CGCCACTGAG AACCGGTGCA GAAACACACC CATGCGTCCA 
CGTTCCCGGA GTCTCAGCCC TGGACGGTCC CCCGCCTGCT GTGACCATGA AATAATTATG 
ATGAACCATG TCTACAAAGA AAGGTTCCCA AAGGCCACAG CTCAGATGGA AGAGCGTCTG 
AAGGAGATCA TCACCAGCTA CTCTCCAGAC CATGTTCTCC CCTTGGCAGA TGGGGTACTT 
AGTTTCACTC ACCATCAGAT CATTGAGCTG GCTCGGGACT GTTTGGATAA ATCTCACCAG 
GGTCTCATCA CGTCGAGATA CTTTTTTGAG TTGCAGCACA AACTGGACAA GTTGCTCCAG 
GAGGCTCACG ATCGCTCTGA AAGTGGAGAA CTGGCATTTA TCAAGCAACT AGTCCGAAAG 
ATCCTAATTG TCATTGCCCG CCCCGCTCGG TTATTGGAGT GTTTGGAATT TGATCCTGAA 
GAATTTTATT ACCTATTGGA AGCTGCAGAA GGCCATGCCA AAGAAGGCCA AGGAATCAAA 
ACTGACATCC CTAGGTATAT CATCAGCCAG CTGGGACTCA ATAAGGACCC TTTAGAAGAA 
ATGGCTCAGT TGGGGAATTA CGACAGTAGG ACAGCAGAGA CACCAGAGAT GGATGAGTCA 
GTGAGTAGCT CAAATACTTC CCTGAGACTT CGAAGGAAAC CCCGAGAGAG TGATTTTGAA 
ACAATTAAAT TGATCAGCAA TGGAGCCTAC GGGGCAGTCT ACTTTGTTCG GCACAAAGAG 
TCCCGCCAGA GGTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTCATCCT TCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGAGACATC CTGACTTTCG CAGAGAACCC CTTTGTGGTC 
AGCATGTATT GCTCCTTTGA AACGAGGCGT CACTTATGCA TGGTCATGGA GTATGTAGAA 
GGGGGAGACT GTGCGACCCT AATGAAAAAC ATGGGACCTC TCCCAGTTGA TATGGCCAGA 
ATGTATTTCG CCGAGACCGT CTTGGCCTTG GAGTACCTGC ATAATTACGG AATCGTACAC 
AGGGACTTGA AGCCAGACAA CCTGTTGGTC ACCTCCATGG GGCACATAAA ACTGACTGAC 
TTCGGCTTGT CTAAGGTGGG ATTAATGAGC ATGACCACCA ATCTCTATGA AGGCCACATA 
GAGAAGGACG CTCGAGAGTT CTTAGATAAA CAGGTCTGTG GTACACCTGA GTACATTGCC 
CCCGAGGTGA TTCTGAGACA GGGCTACGGG AAACCCGTGG ACTGGTGGGC CATGGGCATT 
ATCCTCTATG AATTTCTGGT CGGATGTGTG CCTTTCTTTG GGGACACTCC AGAAGAGCTA 
TTTGGACAAG TCATCAGTGA TGAAATCAAC TGGCCTGAAA AGGACGAGGC CCCTCCTCCA 
GACGCTCAGG AGCTGATTAC CTTGCTCCTC AGGCAGAATC CGCTGGAGAG GCTGGGAACA 
GGTGGAGCCT ATGAAGTGAA GCAGCATCGT TTCTTCCGCT CCTTAGACTG GAACAGTTTG 
CTGAGACAGA AGGCGGAATT TATTCCCCAA CTGGAATCGG AGGATGACAC AAGTTATTTT 
GATACTCGGT CAGAGAAGTA TCATCACATG GAGACGGAGG AGGAGGACGA TACAAACGAT 
GAGGACTTCA CCGTGGAGAT AAGGCAGTTT TCTTCCTGTT CACACAGGTT TTCAAAAGTT 
TTCAGCAGTA TAGATCGCAT AACTCAAAAT TCAGGAGAAG ACAAAGATGA CTCTGAGGAC 
AAGACCAAAA GCACAACGTT GCCATCCACA GAGACACTCA GCTGGAGTTC CGAATACTCT 
GAAATGCAAC AGTTATCGAC CTCCAACTCT TCAGATACTG AAAGCAACAG GTGCAAACTC 
AGCTCTGGCT TGCTCCCCAA GCTGGCTATT TCGACAGATG GGGAACAAGA TGAGGCTGTC 
CCTTGCTCTG GAGACCCCAG AGAGGAGCCA GAGAAACCTG TCCCTCCCTC TGAGGAGTGT 
ACTCAGGAGG AGCCCGAGGT CACCACCCCA GCCAGCACCA TCAGCAGTTC CACACTGTCA 
GTTGGCAGTT TTTCAGAGCA CTTGGATCAG ATAAATGGGC GAAGCGAGTG TGTGGACAGT 
ACAGATAATT CCTCAAAGCC ATCCAGTGAA CCCACTTCTC ACGTGGCTCG ACAGCGCTTA 
GAAAGCACAG AGAAAAAGAA AATTTCTGGG AAAGTCACAA AGTCCCTCTC GGCCAGTGCT 
CTGTCCCTCA TGATCCCAGG AGATATGTTC GCTGTATCTC CATTGGGAAG CCCAATGTCC 
CCACACTCCC TGTCTTCAGA CCCTTCTTCT TCACGGGATT CCTCTCCCAG CCGAGACTCT 
TCTGCAGCAT CTGCCAGTCC GCATCAGCCC ATTGTCATCC ACAGCTCAGG CAAGAACTAT 
GGGTTCACCA TCCGTGCTAT CCGCGTGTAC GTGGGGGACA GTGACATCTA CACAGTGCAC 
CATATCGTCT GGAACGTAGA AGAAGGAAGT CCCGCATACC AGGCAGGACT GAAGGCCGGG 
GATCTGATCA CACACATCAA CGGAGAGCCG GTGCACGGCC TCGTCCACAC GGAAGTTATC 
GAGCTCCTGC TGAAGAGTGG GAATAAGGTG TCTATCACCA CTACTCCATT TGAAAACACA 
TCAATCAAAA CGGGACCAGC CAGGAGAAAC AGTTACAAGG GCCGGATGGT GAGACGAAGC 
AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC 
AAGCAGCCTT CTCCTTTGCT CCACACCAGC CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG 
TCATCTGGAG AGAGCCTCCC GGGTTCCCCA ACTCACAGCT TGTCCCCGAG GTCTCCAACA 
CCCAGTTATC GTTCTACTCC CGATTTTCCG TCAGGTACAA ATTCCTCCCA GAGCAGCTCC 
CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA GGTTCAGGGC ACATCCGGCC CAGCACCCTC 
CATGGCCTGG CTCCCAAACT CAGCGGGCAG CGATACCGCT CTGGAAGACG GAAGTCGGCT 
GGCAGCATCC CTCTCTCCCC GCTGGCCAGG ACACCCTCTC CCACTCCACA GCCTACCTCT 
CCTCAGCGTT CACCATCCCC ACTGTTGGGA CACTCACTGG GCAATGCCAA GATCACTCAG 
GCCTTTCCTA GCAAGATGCA CTCTCCCCCC ACAATCGTCA GACACATCGT GAGGCCCAAG 
AGTGCAGAGC CGCCCCGCTC CCCACTGCTG AAACGGGTGC AGTCGGAGGA AAAGTTGTCA 
CCCTCCTATG GCAGTGACAA GAAGCTTCTG TGCTCCCGCA AGCATAGCCT AGAGGTGACA 
CAAGAGGAGG TACAGAGGGA GCAGTGTCAG CGGGAAGTGA CACTGCAGAG CCTGGAAGAG 
AATGTGTGTG ACGCTCCTTC CCTCAGTCGG GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA 
CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA GAGTCTGTGG ATGACCTGGA CCGGGACAAG 
CTGAAAGCCA AGGTTGTCGT AAAGAAACCA GAAGAGAAAC ATGAATCGCA CCAGAAACCT 
CACAGCCTTG GTGGTGATTC GGAAAGCTAT GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA 
AAAGTGTACT CCAAGGGGTT GGAAAGGTCA GGCCATTTTG AAAACACATC AGCAGAGTTG 
CCTTCTGTGG GCAGCCTGCT GAAGGACACT CTTCACAAGC AGGCCAGCGT GAGGGCCAGC 
GAGGGGGTGA CCTCAGACGG GGCAGCTTGC AGCCTGACAC CAGGGGAGCA CAGCCAGTCT 
CTAGGTGACT TTAAGCGGGC CTCAGCTTCT GGCATTCTCC ATGATAGTGT GTGCCCCATC 
TCTGATAGGC CTGCTCCTGG AAAGGTTGAA TACTCGGAGA AGGCCTCTCA GGCCAAAGAG 
CTCCTTCGAA GTGAAAAACT AGACAGCAAG CTGGCCAATA TTGATTACCT CAGAAAGAAA 
ATGTCATTGG ATGACAAAGA TGACAGCCAC TGTGCCATCC TGAAACCCAA GATAACATCT 
AGCGCCCATG AATGTCTGCC AGGGAACCCC ATACGGCCCA TGGCAGGGCA ACAAGAGACC 
CCGCCAGCCT CTGAAAACCG GGCATTCATC AACAGTACCC ACACACCTCA GATGAGTGCA 
GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT GGCCGGGTAG AGAACGGAGG GGAGAAAGCA 
GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG AAGAGCCCCT CCGAGTATAA GCTAGAGGGC 
AGGTCAGTTT CATGTCTCAA GCCGATCGAG GGCACACTGG ACATTGCTCT CCTGTCTGGA 
CCTCACGCCT CCAAAACAGA GTTGCTTTCC CCAGAGCCTG CACAGAGTCC CAGCCCAGGC 
ATCAACGTGG GACCATGTGT GCCACTAGCT CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC 
TCCACCAGCC TGAGAGAGCC TTCCTCAGCC AACTTAAAAG TAAATAAATC TTATCTGCTG 
GAGCCTCGGT TCCTACCCCC GAGCCGGGCT CTCCAGGACT CTCTCGCAGC CTCTGGGCCA 
GAACCGAAGT CAAAGCCGGA AAGGAAGCTC ATTCATCCTT CTGCCAGGAG CCCAGCAACT 
GTCACAGAGA GCAATCTTCA GCAGAAAGAG GGTGGTCCCG CCACACACCA AGACCGCTCC 
ACTGACACCA GGAACCTCCC TGGCCCAGGG CAGACCCTAC ACAATGTGGA CCTACCCAGG 
CTGTGTACAC GTGCCCCACT CCCACCGGAA GGGACGCCCG CAAAGGAGAA GCCATGTCTG 
AAGGAACCCT CTGCCAAGGT GAAAAGCGAG TGGTCTGCCG TGAGGGATGA CGGACACAGA 
GATCCCTGTG CGAAGCTGTG CCCGGCAGAG ACTGGTAAAG CCAGCGACAG TTCCAAACCC 
CTGCCTTCCG GGGGGAGGAC CCAACCCGAT TTCTACAAGC AGACCCAGAC TTCGGAGAAA 
GCATGGGCGC ATGCAAAAAC AAACCACAAA GATAGCCAAG ATGAGGTGAA GTCCCTGGCC 
AGGGAGGACT CAGCTTCACT TTTATATGAA AAGGAGATAG GCCGGGCACG AAAAGGTCCT 
GAACCCAAAC CGGAAGTTCC TGCTACCCGG TGCCCTCCTC AGCCACCAGG AATTGAGGGT 
GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC TCTTTGCAGA AACAGGCTCC CAAAGAGCCA 
GACAGGAAGG AACAGACTTC GCAAAGGCCT GGAGGTAGTG GCCCTCAACA ACCCCCACCC 
ACCAAAGAGC TGTCTAACTC AGCATCCTGG CAGCACGGCA GTTCTCCGAG TCACACTTTA 
AAGAAGGAGC CCGGGACCAA AGCTGCCGCT GCAGAACCAA GCACCAGCCT TCATGACACT 
CCCCGATCTG CTACAGCCAC CACCACTGCC ATTGCCACCA CCACCACTAC CACCAGTGCC 
GGGCACAGTG ACTGCAGTAG CCATAAGGCC CGGCCTGGCC CTGACCCCAG CCCTTCAAAG 
TCTAAGCACC AAGACAGGTC CCTCTCCTCA CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC 
AAAGAGCCTG TCACTCAACC CCTGGGTGGT TCCATCAGAG AAGGCAAGGG TGGCAGCAAG 
GGTCCAGTGG ACACATTTTC TGCTGTCCTG ACCACCCAGG GCAAAGCAAG TGATGTGCTT 
GTGCAGGGAG AAGGTCGGGT CTCAATCATT GTCCACACTG AAGAGTGTCC TCTCGATGCC 
AAACTGAAAA ACACCAATGG AGGGTGTCCC CCAGAGATGC AGGCGAAGCA TCCACCCAGA 
CAAGGACATC TCAGTGAAGC AGCAGACCAG AAGCCACTCA TTGCTGGTGA GAAGCAAAGC 
CCGTCTCCAA AGCATCCCAA ACCATCCACT GTGAAAGATT ACCCCAGTCT GTGCAGACAG 
ACAGACAGAA GCCCAAGCCA TCAGGCTACC ACTGGGGACA GGAAGGCAGA AGGAAAGAAA 
TGCACAGACG CACTTTATGT CGCAGCCCCA GAGGGCTACA AGCCAGAGGC CAGCCCTTCT 
CTCCACCACG GCGAGACCGG ACTCAGAGGC TCAGAGAGGC CACCCATGGG CATGGGGAAG 
GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA GGTCCCCAGA AGTCACTGGC TGAAACAGGC 
AAGCCCAGCG GTATGAAAAG GTCACCCTCT GCCACCGTGC AGAGCTCTCT CCGCTCAGCT 
GCCCCCCCAG AAAAGTCTCT GAGTTACTCA GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG 
CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC TCATCTGCCA AGGCTACAGG GGGGACCTCA 
GAGTTCCCAG CCCCCAGCAG CAGGGACCAC AGGAAGCTTC AGTCTGGAGG AGACGGCCGA 
AGCCAAATGA TAAAGAGTGA CTCTCTGCCC TCCTTCCGCC TCTCCACCTC TGCTCTGGAG 
TCACATTTCC AGGATCCACA GGTGCCCATC GCATCAGGCC ACCGAGGCAG GGCACTGTCA 
GTAACTGCTG CCACAGGAGA ACCCAAAGGG AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG 
AAACAGAATG CGTGCAGAGA GGCGACCAGA GCACCCCCAG CCCCAAGCAC AGATCGCTCC 
CTCCCTCTTT CCTCAGAGAA AGACTTCGTG GTTCGGCAGA GAAGGGGCAA GGAGACCTTA 
AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_006517704.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517767.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X15, mRNA.

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CloneID	OMu49080
Clone ID Related Accession (Same CDS sequence)	XM_006517704.4 , XM_006517704.3
Accession Version	XM_006517704.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517704.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGA AGGAGCTGAG CCTCCCCCGA 
AGAGGAAGTC TTTGCCGAAC AAGCAACCGG AAGAGTTTGA TAGGCAATGG CCAGTCTCCA 
GCACTGCCTC GACCACACTC ACCTCTCTCT GCTCATGCAG GAAATAGCCC TCAAGACAGT 
CCAAGGAATT TCTCCCCCAG TGCCTCAGCC CATTTCTCAT TTGCGAGGAG AAATGACAGA 
ACGGATGGGC GCCGCTGGTC TCTGGCTTCT CTCCCTTCCT CAGGCTATGG GACAAATACC 
CCAAGCTCCA CCGTCTCCTC ATCCTGTTCT TCCCAGGAGA AGTTGCACCA GCTACCATAC 
CAGCCAACCC CAGATGAATT ACACTTCTTA TCCAAACACT TCTGCACAAC AGAAAGTATC 
GCCACTGAGA ACCGGTGCAG AAACACACCC ATGCGTCCAC GTTCCCGGAG TCTCAGCCCT 
GGACGGTCCC CCGCCTGCTG TGACCATGAA ATAATTATGA TGAACCATGT CTACAAAGAA 
AGGTTCCCAA AGGCCACAGC TCAGATGGAA GAGCGTCTGA AGGAGATCAT CACCAGCTAC 
TCTCCAGACC ATGTTCTCCC CTTGGCAGAT GGGGTACTTA GTTTCACTCA CCATCAGATC 
ATTGAGCTGG CTCGGGACTG TTTGGATAAA TCTCACCAGG GTCTCATCAC GTCGAGATAC 
TTTTTTGAGT TGCAGCACAA ACTGGACAAG TTGCTCCAGG AGGCTCACGA TCGCTCTGAA 
AGTGGAGAAC TGGCATTTAT CAAGCAACTA GTCCGAAAGA TCCTAATTGT CATTGCCCGC 
CCCGCTCGGT TATTGGAGTG TTTGGAATTT GATCCTGAAG AATTTTATTA CCTATTGGAA 
GCTGCAGAAG GCCATGCCAA AGAAGGCCAA GGAATCAAAA CTGACATCCC TAGGTATATC 
ATCAGCCAGC TGGGACTCAA TAAGGACCCT TTAGAAGAAA TGGCTCAGTT GGGGAATTAC 
GACAGTAGGA CAGCAGAGAC ACCAGAGATG GATGAGTCAG TGAGTAGCTC AAATACTTCC 
CTGAGACTTC GAAGGAAACC CCGAGAGAGT GATTTTGAAA CAATTAAATT GATCAGCAAT 
GGAGCCTACG GGGCAGTCTA CTTTGTTCGG CACAAAGAGT CCCGCCAGAG GTTTGCCATG 
AAGAAGATCA ACAAGCAGAA CCTCATCCTT CGGAACCAGA TCCAGCAGGC CTTCGTGGAG 
CGAGACATCC TGACTTTCGC AGAGAACCCC TTTGTGGTCA GCATGTATTG CTCCTTTGAA 
ACGAGGCGTC ACTTATGCAT GGTCATGGAG TATGTAGAAG GGGGAGACTG TGCGACCCTA 
ATGAAAAACA TGGGACCTCT CCCAGTTGAT ATGGCCAGAA TGTATTTCGC CGAGACCGTC 
TTGGCCTTGG AGTACCTGCA TAATTACGGA ATCGTACACA GGGACTTGAA GCCAGACAAC 
CTGTTGGTCA CCTCCATGGG GCACATAAAA CTGACTGACT TCGGCTTGTC TAAGGTGGGA 
TTAATGAGCA TGACCACCAA TCTCTATGAA GGCCACATAG AGAAGGACGC TCGAGAGTTC 
TTAGATAAAC AGGTCTGTGG TACACCTGAG TACATTGCCC CCGAGGTGAT TCTGAGACAG 
GGCTACGGGA AACCCGTGGA CTGGTGGGCC ATGGGCATTA TCCTCTATGA ATTTCTGGTC 
GGATGTGTGC CTTTCTTTGG GGACACTCCA GAAGAGCTAT TTGGACAAGT CATCAGTGAT 
GAAATCAACT GGCCTGAAAA GGACGAGGCC CCTCCTCCAG ACGCTCAGGA GCTGATTACC 
TTGCTCCTCA GGCAGAATCC GCTGGAGAGG CTGGGAACAG GTGGAGCCTA TGAAGTGAAG 
CAGCATCGTT TCTTCCGCTC CTTAGACTGG AACAGTTTGC TGAGACAGAA GGCGGAATTT 
ATTCCCCAAC TGGAATCGGA GGATGACACA AGTTATTTTG ATACTCGGTC AGAGAAGTAT 
CATCACATGG AGACGGAGGA GGAGGACGAT ACAAACGATG AGGACTTCAC CGTGGAGATA 
AGGCAGTTTT CTTCCTGTTC ACACAGGTTT TCAAAAGTTT TCAGCAGTAT AGATCGCATA 
ACTCAAAATT CAGGAGAAGA CAAAGATGAC TCTGAGGACA AGACCAAAAG CACAACGTTG 
CCATCCACAG AGACACTCAG CTGGAGTTCC GAATACTCTG AAATGCAACA GTTATCGACC 
TCCAACTCTT CAGATACTGA AAGCAACAGG TGCAAACTCA GCTCTGGCTT GCTCCCCAAG 
CTGGCTATTT CGACAGATGG GGAACAAGAT GAGGCTGTCC CTTGCTCTGG AGACCCCAGA 
GAGGAGCCAG AGAAACCTGT CCCTCCCTCT GAGGAGTGTA CTCAGGAGGA GCCCGAGGTC 
ACCACCCCAG CCAGCACCAT CAGCAGTTCC ACACTGTCAG TTGGCAGTTT TTCAGAGCAC 
TTGGATCAGA TAAATGGGCG AAGCGAGTGT GTGGACAGTA CAGATAATTC CTCAAAGCCA 
TCCAGTGAAC CCACTTCTCA CGTGGCTCGA CAGCGCTTAG AAAGCACAGA GAAAAAGAAA 
ATTTCTGGGA AAGTCACAAA GTCCCTCTCG GCCAGTGCTC TGTCCCTCAT GATCCCAGGA 
GATATGTTCG CTGTATCTCC ATTGGGAAGC CCAATGTCCC CACACTCCCT GTCTTCAGAC 
CCTTCTTCTT CACGGGATTC CTCTCCCAGC CGAGACTCTT CTGCAGCATC TGCCAGTCCG 
CATCAGCCCA TTGTCATCCA CAGCTCAGGC AAGAACTATG GGTTCACCAT CCGTGCTATC 
CGCGTGTACG TGGGGGACAG TGACATCTAC ACAGTGCACC ATATCGTCTG GAACGTAGAA 
GAAGGAAGTC CCGCATACCA GGCAGGACTG AAGGCCGGGG ATCTGATCAC ACACATCAAC 
GGAGAGCCGG TGCACGGCCT CGTCCACACG GAAGTTATCG AGCTCCTGCT GAAGAGTGGG 
AATAAGGTGT CTATCACCAC TACTCCATTT GAAAACACAT CAATCAAAAC GGGACCAGCC 
AGGAGAAACA GTTACAAGGG CCGGATGGTG AGACGAAGCA AGAAGTCCAA GAAGAAGGAG 
AGTCTAGAAA GGAGGAGATC TCTCTTCAAG AAGCTGGCCA AGCAGCCTTC TCCTTTGCTC 
CACACCAGCC GAAGTTTCTC CTGCTTAAAC CGGTCCCTGT CATCTGGAGA GAGCCTCCCG 
GGTTCCCCAA CTCACAGCTT GTCCCCGAGG TCTCCAACAC CCAGTTATCG TTCTACTCCC 
GATTTTCCGT CAGGTACAAA TTCCTCCCAG AGCAGCTCCC CAAGTTCAAG TGCCCCCAAT 
TCTCCAGCAG GTTCAGGGCA CATCCGGCCC AGCACCCTCC ATGGCCTGGC TCCCAAACTC 
AGCGGGCAGC GATACCGCTC TGGAAGACGG AAGTCGGCTG GCAGCATCCC TCTCTCCCCG 
CTGGCCAGGA CACCCTCTCC CACTCCACAG CCTACCTCTC CTCAGCGTTC ACCATCCCCA 
CTGTTGGGAC ACTCACTGGG CAATGCCAAG ATCACTCAGG CCTTTCCTAG CAAGATGCAC 
TCTCCCCCCA CAATCGTCAG ACACATCGTG AGGCCCAAGA GTGCAGAGCC GCCCCGCTCC 
CCACTGCTGA AACGGGTGCA GTCGGAGGAA AAGTTGTCAC CCTCCTATGG CAGTGACAAG 
AAGCTTCTGT GCTCCCGCAA GCATAGCCTA GAGGTGACAC AAGAGGAGGT ACAGAGGGAG 
CAGTGTCAGC GGGAAGTGAC ACTGCAGAGC CTGGAAGAGA ATGTGTGTGA CGCTCCTTCC 
CTCAGTCGGG CCAGGCCAGT GGAGCAAGGC TGTCTGAAAC GCCCCGTGTC CCGGAAGGTG 
GGCAGGCAAG AGTCTGTGGA TGACCTGGAC CGGGACAAGC TGAAAGCCAA GGTTGTCGTA 
AAGAAACCAG AAGAGAAACA TGAATCGCAC CAGAAACCTC ACAGCCTTGG TGGTGATTCG 
GAAAGCTATG CTCTCTTCAG GCTAGAGGAG AGAGAGAAAA AAGTGTACTC CAAGGGGTTG 
GAAAGGTCAG GCCATTTTGA AAACACATCA GCAGAGTTGC CTTCTGTGGG CAGCCTGCTG 
AAGGACACTC TTCACAAGCA GGCCAGCGTG AGGGCCAGCG AGGGGGTGAC CTCAGACGGG 
GCAGCTTGCA GCCTGACACC AGGGGAGCAC AGCCAGTCTC TAGGTGACTT TAAGCGGGCC 
TCAGCTTCTG GCATTCTCCA TGATAGTGTG TGCCCCATCT CTGATAGGCC TGCTCCTGGA 
AAGGTTGAAT ACTCGGAGAA GGCCTCTCAG GCCAAAGAGC TCCTTCGAAG TGAAAAACTA 
GACAGCAAGC TGGCCAATAT TGATTACCTC AGAAAGAAAA TGTCATTGGA TGACAAAGAT 
GACAGCCACT GTGCCATCCT GAAACCCAAG ATAACATCTA GCGCCCATGA ATGTCTGCCA 
GGGAACCCCA TACGGCCCAT GGCAGGGCAA CAAGAGACCC CGCCAGCCTC TGAAAACCGG 
GCATTCATCA ACAGTACCCA CACACCTCAG ATGAGTGCAG TTTCCTTTGT TCCTCTCAAA 
GCCTTAGCTG GCCGGGTAGA GAACGGAGGG GAGAAAGCAG GCTTAGCTGC TCCCGAGTCC 
CCTGTCAGGA AGAGCCCCTC CGAGTATAAG CTAGAGGGCA GGTCAGTTTC ATGTCTCAAG 
CCGATCGAGG GCACACTGGA CATTGCTCTC CTGTCTGGAC CTCACGCCTC CAAAACAGAG 
TTGCTTTCCC CAGAGCCTGC ACAGAGTCCC AGCCCAGGCA TCAACGTGGG ACCATGTGTG 
CCACTAGCTC TTCCTGGGAG CAGTGGGAAA AAGGGAGACT CCACCAGCCT GAGAGAGCCT 
TCCTCAGCCA ACTTAAAAGT AAATAAATCT TATCTGCTGG AGCCTCGGTT CCTACCCCCG 
AGCCGGGCTC TCCAGGACTC TCTCGCAGCC TCTGGGCCAG AACCGAAGTC AAAGCCGGAA 
AGGAAGCTCA TTCATCCTTC TGCCAGGAGC CCAGCAACTG TCACAGAGAG CAATCTTCAG 
CAGAAAGAGG GTGGTCCCGC CACACACCAA GACCGCTCCA CTGACACCAG GAACCTCCCT 
GGCCCAGGGC AGACCCTACA CAATGTGGAC CTACCCAGGC TGTGTACACG TGCCCCACTC 
CCACCGGAAG GGACGCCCGC AAAGGAGAAG CCATGTCTGA AGGAACCCTC TGCCAAGGTG 
AAAAGCGAGT GGTCTGCCGT GAGGGATGAC GGACACAGAG ATCCCTGTGC GAAGCTGTGC 
CCGGCAGAGA CTGGTAAAGC CAGCGACAGT TCCAAACCCC TGCCTTCCGG GGGGAGGACC 
CAACCCGATT TCTACAAGCA GACCCAGACT TCGGAGAAAG CATGGGCGCA TGCAAAAACA 
AACCACAAAG ATAGCCAAGA TGAGGTGAAG TCCCTGGCCA GGGAGGACTC AGCTTCACTT 
TTATATGAAA AGGAGATAGG CCGGGCACGA AAAGGTCCTG AACCCAAACC GGAAGTTCCT 
GCTACCCGGT GCCCTCCTCA GCCACCAGGA ATTGAGGGTG AGAAGCGAGA AAAGCTCTCC 
GCTGCCCCCT CTTTGCAGAA ACAGGCTCCC AAAGAGCCAG ACAGGAAGGA ACAGACTTCG 
CAAAGGCCTG GAGGTAGTGG CCCTCAACAA CCCCCACCCA CCAAAGAGCT GTCTAACTCA 
GCATCCTGGC AGCACGGCAG TTCTCCGAGT CACACTTTAA AGAAGGAGCC CGGGACCAAA 
GCTGCCGCTG CAGAACCAAG CACCAGCCTT CATGACACTC CCCGATCTGC TACAGCCACC 
ACCACTGCCA TTGCCACCAC CACCACTACC ACCAGTGCCG GGCACAGTGA CTGCAGTAGC 
CATAAGGCCC GGCCTGGCCC TGACCCCAGC CCTTCAAAGT CTAAGCACCA AGACAGGTCC 
CTCTCCTCAC AGAAGCTGAG TGCTGGCTCT GCAAAAGGCA AAGAGCCTGT CACTCAACCC 
CTGGGTGGTT CCATCAGAGA AGGCAAGGGT GGCAGCAAGG GTCCAGTGGA CACATTTTCT 
GCTGTCCTGA CCACCCAGGG CAAAGCAAGT GATGTGCTTG TGCAGGGAGA AGGTCGGGTC 
TCAATCATTG TCCACACTGA AGAGTGTCCT CTCGATGCCA AACTGAAAAA CACCAATGGA 
GGGTGTCCCC CAGAGATGCA GGCGAAGCAT CCACCCAGAC AAGGACATCT CAGTGAAGCA 
GCAGACCAGA AGCCACTCAT TGCTGGTGAG AAGCAAAGCC CGTCTCCAAA GCATCCCAAA 
CCATCCACTG TGAAAGATTA CCCCAGTCTG TGCAGACAGA CAGACAGAAG CCCAAGCCAT 
CAGGCTACCA CTGGGGACAG GAAGGCAGAA GGAAAGAAAT GCACAGACGC ACTTTATGTC 
GCAGCCCCAG AGGGCTACAA GCCAGAGGCC AGCCCTTCTC TCCACCACGG CGAGACCGGA 
CTCAGAGGCT CAGAGAGGCC ACCCATGGGC ATGGGGAAGG GCTTCTCTGA GCCCAAGGGG 
AAAGGGCCAG GTCCCCAGAA GTCACTGGCT GAAACAGGCA AGCCCAGCGG TATGAAAAGG 
TCACCCTCTG CCACCGTGCA GAGCTCTCTC CGCTCAGCTG CCCCCCCAGA AAAGTCTCTG 
AGTTACTCAG CCAGCTTTCC CGAGGCCCAG CCTGGAGTGC GAGAGGTCCC TGCAGCCAAC 
AGCAGCCCCT CATCTGCCAA GGCTACAGGG GGGACCTCAG AGTTCCCAGC CCCCAGCAGC 
AGGGACCACA GGAAGCTTCA GTCTGGAGGA GACGGCCGAA GCCAAATGAT AAAGAGTGAC 
TCTCTGCCCT CCTTCCGCCT CTCCACCTCT GCTCTGGAGT CACATTTCCA GGATCCACAG 
GTGCCCATCG CATCAGGCCA CCGAGGCAGG GCACTGTCAG TAACTGCTGC CACAGGAGAA 
CCCAAAGGGA GAGAGCTCGC CCAGCCTCCC CCAGTCAGGA AACAGAATGC GTGCAGAGAG 
GCGACCAGAG CACCCCCAGC CCCAAGCACA GATCGCTCCC TCCCTCTTTC CTCAGAGAAA 
GACTTCGTGG TTCGGCAGAG AAGGGGCAAG GAGACCTTAA GGAGCAGTCC TCACAAAAAG 
GCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517767.1
CDS	106..7374
Translation	MDESSILRRRGLQKELSLPRRGSLCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_006517704.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517704.4

ORF Insert Sequence:

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGA AGGAGCTGAG CCTCCCCCGA 
AGAGGAAGTC TTTGCCGAAC AAGCAACCGG AAGAGTTTGA TAGGCAATGG CCAGTCTCCA 
GCACTGCCTC GACCACACTC ACCTCTCTCT GCTCATGCAG GAAATAGCCC TCAAGACAGT 
CCAAGGAATT TCTCCCCCAG TGCCTCAGCC CATTTCTCAT TTGCGAGGAG AAATGACAGA 
ACGGATGGGC GCCGCTGGTC TCTGGCTTCT CTCCCTTCCT CAGGCTATGG GACAAATACC 
CCAAGCTCCA CCGTCTCCTC ATCCTGTTCT TCCCAGGAGA AGTTGCACCA GCTACCATAC 
CAGCCAACCC CAGATGAATT ACACTTCTTA TCCAAACACT TCTGCACAAC AGAAAGTATC 
GCCACTGAGA ACCGGTGCAG AAACACACCC ATGCGTCCAC GTTCCCGGAG TCTCAGCCCT 
GGACGGTCCC CCGCCTGCTG TGACCATGAA ATAATTATGA TGAACCATGT CTACAAAGAA 
AGGTTCCCAA AGGCCACAGC TCAGATGGAA GAGCGTCTGA AGGAGATCAT CACCAGCTAC 
TCTCCAGACC ATGTTCTCCC CTTGGCAGAT GGGGTACTTA GTTTCACTCA CCATCAGATC 
ATTGAGCTGG CTCGGGACTG TTTGGATAAA TCTCACCAGG GTCTCATCAC GTCGAGATAC 
TTTTTTGAGT TGCAGCACAA ACTGGACAAG TTGCTCCAGG AGGCTCACGA TCGCTCTGAA 
AGTGGAGAAC TGGCATTTAT CAAGCAACTA GTCCGAAAGA TCCTAATTGT CATTGCCCGC 
CCCGCTCGGT TATTGGAGTG TTTGGAATTT GATCCTGAAG AATTTTATTA CCTATTGGAA 
GCTGCAGAAG GCCATGCCAA AGAAGGCCAA GGAATCAAAA CTGACATCCC TAGGTATATC 
ATCAGCCAGC TGGGACTCAA TAAGGACCCT TTAGAAGAAA TGGCTCAGTT GGGGAATTAC 
GACAGTAGGA CAGCAGAGAC ACCAGAGATG GATGAGTCAG TGAGTAGCTC AAATACTTCC 
CTGAGACTTC GAAGGAAACC CCGAGAGAGT GATTTTGAAA CAATTAAATT GATCAGCAAT 
GGAGCCTACG GGGCAGTCTA CTTTGTTCGG CACAAAGAGT CCCGCCAGAG GTTTGCCATG 
AAGAAGATCA ACAAGCAGAA CCTCATCCTT CGGAACCAGA TCCAGCAGGC CTTCGTGGAG 
CGAGACATCC TGACTTTCGC AGAGAACCCC TTTGTGGTCA GCATGTATTG CTCCTTTGAA 
ACGAGGCGTC ACTTATGCAT GGTCATGGAG TATGTAGAAG GGGGAGACTG TGCGACCCTA 
ATGAAAAACA TGGGACCTCT CCCAGTTGAT ATGGCCAGAA TGTATTTCGC CGAGACCGTC 
TTGGCCTTGG AGTACCTGCA TAATTACGGA ATCGTACACA GGGACTTGAA GCCAGACAAC 
CTGTTGGTCA CCTCCATGGG GCACATAAAA CTGACTGACT TCGGCTTGTC TAAGGTGGGA 
TTAATGAGCA TGACCACCAA TCTCTATGAA GGCCACATAG AGAAGGACGC TCGAGAGTTC 
TTAGATAAAC AGGTCTGTGG TACACCTGAG TACATTGCCC CCGAGGTGAT TCTGAGACAG 
GGCTACGGGA AACCCGTGGA CTGGTGGGCC ATGGGCATTA TCCTCTATGA ATTTCTGGTC 
GGATGTGTGC CTTTCTTTGG GGACACTCCA GAAGAGCTAT TTGGACAAGT CATCAGTGAT 
GAAATCAACT GGCCTGAAAA GGACGAGGCC CCTCCTCCAG ACGCTCAGGA GCTGATTACC 
TTGCTCCTCA GGCAGAATCC GCTGGAGAGG CTGGGAACAG GTGGAGCCTA TGAAGTGAAG 
CAGCATCGTT TCTTCCGCTC CTTAGACTGG AACAGTTTGC TGAGACAGAA GGCGGAATTT 
ATTCCCCAAC TGGAATCGGA GGATGACACA AGTTATTTTG ATACTCGGTC AGAGAAGTAT 
CATCACATGG AGACGGAGGA GGAGGACGAT ACAAACGATG AGGACTTCAC CGTGGAGATA 
AGGCAGTTTT CTTCCTGTTC ACACAGGTTT TCAAAAGTTT TCAGCAGTAT AGATCGCATA 
ACTCAAAATT CAGGAGAAGA CAAAGATGAC TCTGAGGACA AGACCAAAAG CACAACGTTG 
CCATCCACAG AGACACTCAG CTGGAGTTCC GAATACTCTG AAATGCAACA GTTATCGACC 
TCCAACTCTT CAGATACTGA AAGCAACAGG TGCAAACTCA GCTCTGGCTT GCTCCCCAAG 
CTGGCTATTT CGACAGATGG GGAACAAGAT GAGGCTGTCC CTTGCTCTGG AGACCCCAGA 
GAGGAGCCAG AGAAACCTGT CCCTCCCTCT GAGGAGTGTA CTCAGGAGGA GCCCGAGGTC 
ACCACCCCAG CCAGCACCAT CAGCAGTTCC ACACTGTCAG TTGGCAGTTT TTCAGAGCAC 
TTGGATCAGA TAAATGGGCG AAGCGAGTGT GTGGACAGTA CAGATAATTC CTCAAAGCCA 
TCCAGTGAAC CCACTTCTCA CGTGGCTCGA CAGCGCTTAG AAAGCACAGA GAAAAAGAAA 
ATTTCTGGGA AAGTCACAAA GTCCCTCTCG GCCAGTGCTC TGTCCCTCAT GATCCCAGGA 
GATATGTTCG CTGTATCTCC ATTGGGAAGC CCAATGTCCC CACACTCCCT GTCTTCAGAC 
CCTTCTTCTT CACGGGATTC CTCTCCCAGC CGAGACTCTT CTGCAGCATC TGCCAGTCCG 
CATCAGCCCA TTGTCATCCA CAGCTCAGGC AAGAACTATG GGTTCACCAT CCGTGCTATC 
CGCGTGTACG TGGGGGACAG TGACATCTAC ACAGTGCACC ATATCGTCTG GAACGTAGAA 
GAAGGAAGTC CCGCATACCA GGCAGGACTG AAGGCCGGGG ATCTGATCAC ACACATCAAC 
GGAGAGCCGG TGCACGGCCT CGTCCACACG GAAGTTATCG AGCTCCTGCT GAAGAGTGGG 
AATAAGGTGT CTATCACCAC TACTCCATTT GAAAACACAT CAATCAAAAC GGGACCAGCC 
AGGAGAAACA GTTACAAGGG CCGGATGGTG AGACGAAGCA AGAAGTCCAA GAAGAAGGAG 
AGTCTAGAAA GGAGGAGATC TCTCTTCAAG AAGCTGGCCA AGCAGCCTTC TCCTTTGCTC 
CACACCAGCC GAAGTTTCTC CTGCTTAAAC CGGTCCCTGT CATCTGGAGA GAGCCTCCCG 
GGTTCCCCAA CTCACAGCTT GTCCCCGAGG TCTCCAACAC CCAGTTATCG TTCTACTCCC 
GATTTTCCGT CAGGTACAAA TTCCTCCCAG AGCAGCTCCC CAAGTTCAAG TGCCCCCAAT 
TCTCCAGCAG GTTCAGGGCA CATCCGGCCC AGCACCCTCC ATGGCCTGGC TCCCAAACTC 
AGCGGGCAGC GATACCGCTC TGGAAGACGG AAGTCGGCTG GCAGCATCCC TCTCTCCCCG 
CTGGCCAGGA CACCCTCTCC CACTCCACAG CCTACCTCTC CTCAGCGTTC ACCATCCCCA 
CTGTTGGGAC ACTCACTGGG CAATGCCAAG ATCACTCAGG CCTTTCCTAG CAAGATGCAC 
TCTCCCCCCA CAATCGTCAG ACACATCGTG AGGCCCAAGA GTGCAGAGCC GCCCCGCTCC 
CCACTGCTGA AACGGGTGCA GTCGGAGGAA AAGTTGTCAC CCTCCTATGG CAGTGACAAG 
AAGCTTCTGT GCTCCCGCAA GCATAGCCTA GAGGTGACAC AAGAGGAGGT ACAGAGGGAG 
CAGTGTCAGC GGGAAGTGAC ACTGCAGAGC CTGGAAGAGA ATGTGTGTGA CGCTCCTTCC 
CTCAGTCGGG CCAGGCCAGT GGAGCAAGGC TGTCTGAAAC GCCCCGTGTC CCGGAAGGTG 
GGCAGGCAAG AGTCTGTGGA TGACCTGGAC CGGGACAAGC TGAAAGCCAA GGTTGTCGTA 
AAGAAACCAG AAGAGAAACA TGAATCGCAC CAGAAACCTC ACAGCCTTGG TGGTGATTCG 
GAAAGCTATG CTCTCTTCAG GCTAGAGGAG AGAGAGAAAA AAGTGTACTC CAAGGGGTTG 
GAAAGGTCAG GCCATTTTGA AAACACATCA GCAGAGTTGC CTTCTGTGGG CAGCCTGCTG 
AAGGACACTC TTCACAAGCA GGCCAGCGTG AGGGCCAGCG AGGGGGTGAC CTCAGACGGG 
GCAGCTTGCA GCCTGACACC AGGGGAGCAC AGCCAGTCTC TAGGTGACTT TAAGCGGGCC 
TCAGCTTCTG GCATTCTCCA TGATAGTGTG TGCCCCATCT CTGATAGGCC TGCTCCTGGA 
AAGGTTGAAT ACTCGGAGAA GGCCTCTCAG GCCAAAGAGC TCCTTCGAAG TGAAAAACTA 
GACAGCAAGC TGGCCAATAT TGATTACCTC AGAAAGAAAA TGTCATTGGA TGACAAAGAT 
GACAGCCACT GTGCCATCCT GAAACCCAAG ATAACATCTA GCGCCCATGA ATGTCTGCCA 
GGGAACCCCA TACGGCCCAT GGCAGGGCAA CAAGAGACCC CGCCAGCCTC TGAAAACCGG 
GCATTCATCA ACAGTACCCA CACACCTCAG ATGAGTGCAG TTTCCTTTGT TCCTCTCAAA 
GCCTTAGCTG GCCGGGTAGA GAACGGAGGG GAGAAAGCAG GCTTAGCTGC TCCCGAGTCC 
CCTGTCAGGA AGAGCCCCTC CGAGTATAAG CTAGAGGGCA GGTCAGTTTC ATGTCTCAAG 
CCGATCGAGG GCACACTGGA CATTGCTCTC CTGTCTGGAC CTCACGCCTC CAAAACAGAG 
TTGCTTTCCC CAGAGCCTGC ACAGAGTCCC AGCCCAGGCA TCAACGTGGG ACCATGTGTG 
CCACTAGCTC TTCCTGGGAG CAGTGGGAAA AAGGGAGACT CCACCAGCCT GAGAGAGCCT 
TCCTCAGCCA ACTTAAAAGT AAATAAATCT TATCTGCTGG AGCCTCGGTT CCTACCCCCG 
AGCCGGGCTC TCCAGGACTC TCTCGCAGCC TCTGGGCCAG AACCGAAGTC AAAGCCGGAA 
AGGAAGCTCA TTCATCCTTC TGCCAGGAGC CCAGCAACTG TCACAGAGAG CAATCTTCAG 
CAGAAAGAGG GTGGTCCCGC CACACACCAA GACCGCTCCA CTGACACCAG GAACCTCCCT 
GGCCCAGGGC AGACCCTACA CAATGTGGAC CTACCCAGGC TGTGTACACG TGCCCCACTC 
CCACCGGAAG GGACGCCCGC AAAGGAGAAG CCATGTCTGA AGGAACCCTC TGCCAAGGTG 
AAAAGCGAGT GGTCTGCCGT GAGGGATGAC GGACACAGAG ATCCCTGTGC GAAGCTGTGC 
CCGGCAGAGA CTGGTAAAGC CAGCGACAGT TCCAAACCCC TGCCTTCCGG GGGGAGGACC 
CAACCCGATT TCTACAAGCA GACCCAGACT TCGGAGAAAG CATGGGCGCA TGCAAAAACA 
AACCACAAAG ATAGCCAAGA TGAGGTGAAG TCCCTGGCCA GGGAGGACTC AGCTTCACTT 
TTATATGAAA AGGAGATAGG CCGGGCACGA AAAGGTCCTG AACCCAAACC GGAAGTTCCT 
GCTACCCGGT GCCCTCCTCA GCCACCAGGA ATTGAGGGTG AGAAGCGAGA AAAGCTCTCC 
GCTGCCCCCT CTTTGCAGAA ACAGGCTCCC AAAGAGCCAG ACAGGAAGGA ACAGACTTCG 
CAAAGGCCTG GAGGTAGTGG CCCTCAACAA CCCCCACCCA CCAAAGAGCT GTCTAACTCA 
GCATCCTGGC AGCACGGCAG TTCTCCGAGT CACACTTTAA AGAAGGAGCC CGGGACCAAA 
GCTGCCGCTG CAGAACCAAG CACCAGCCTT CATGACACTC CCCGATCTGC TACAGCCACC 
ACCACTGCCA TTGCCACCAC CACCACTACC ACCAGTGCCG GGCACAGTGA CTGCAGTAGC 
CATAAGGCCC GGCCTGGCCC TGACCCCAGC CCTTCAAAGT CTAAGCACCA AGACAGGTCC 
CTCTCCTCAC AGAAGCTGAG TGCTGGCTCT GCAAAAGGCA AAGAGCCTGT CACTCAACCC 
CTGGGTGGTT CCATCAGAGA AGGCAAGGGT GGCAGCAAGG GTCCAGTGGA CACATTTTCT 
GCTGTCCTGA CCACCCAGGG CAAAGCAAGT GATGTGCTTG TGCAGGGAGA AGGTCGGGTC 
TCAATCATTG TCCACACTGA AGAGTGTCCT CTCGATGCCA AACTGAAAAA CACCAATGGA 
GGGTGTCCCC CAGAGATGCA GGCGAAGCAT CCACCCAGAC AAGGACATCT CAGTGAAGCA 
GCAGACCAGA AGCCACTCAT TGCTGGTGAG AAGCAAAGCC CGTCTCCAAA GCATCCCAAA 
CCATCCACTG TGAAAGATTA CCCCAGTCTG TGCAGACAGA CAGACAGAAG CCCAAGCCAT 
CAGGCTACCA CTGGGGACAG GAAGGCAGAA GGAAAGAAAT GCACAGACGC ACTTTATGTC 
GCAGCCCCAG AGGGCTACAA GCCAGAGGCC AGCCCTTCTC TCCACCACGG CGAGACCGGA 
CTCAGAGGCT CAGAGAGGCC ACCCATGGGC ATGGGGAAGG GCTTCTCTGA GCCCAAGGGG 
AAAGGGCCAG GTCCCCAGAA GTCACTGGCT GAAACAGGCA AGCCCAGCGG TATGAAAAGG 
TCACCCTCTG CCACCGTGCA GAGCTCTCTC CGCTCAGCTG CCCCCCCAGA AAAGTCTCTG 
AGTTACTCAG CCAGCTTTCC CGAGGCCCAG CCTGGAGTGC GAGAGGTCCC TGCAGCCAAC 
AGCAGCCCCT CATCTGCCAA GGCTACAGGG GGGACCTCAG AGTTCCCAGC CCCCAGCAGC 
AGGGACCACA GGAAGCTTCA GTCTGGAGGA GACGGCCGAA GCCAAATGAT AAAGAGTGAC 
TCTCTGCCCT CCTTCCGCCT CTCCACCTCT GCTCTGGAGT CACATTTCCA GGATCCACAG 
GTGCCCATCG CATCAGGCCA CCGAGGCAGG GCACTGTCAG TAACTGCTGC CACAGGAGAA 
CCCAAAGGGA GAGAGCTCGC CCAGCCTCCC CCAGTCAGGA AACAGAATGC GTGCAGAGAG 
GCGACCAGAG CACCCCCAGC CCCAAGCACA GATCGCTCCC TCCCTCTTTC CTCAGAGAAA 
GACTTCGTGG TTCGGCAGAG AAGGGGCAAG GAGACCTTAA GGAGCAGTCC TCACAAAAAG 
GCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_006517705.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517768.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X16, mRNA.

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CloneID	OMu49081
Clone ID Related Accession (Same CDS sequence)	XM_006517705.4 , XM_006517705.3
Accession Version	XM_006517705.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517705.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGA AGGAGCTGAG CCTCCCCCGA 
AGAGGAAGTC TTTGCCGAAC AAGCAACCGG AAGAGTTTGA TAGGCAATGG CCAGTCTCCA 
GCACTGCCTC GACCACACTC ACCTCTCTCT GCTCATGCAG GAAATAGCCC TCAAGACAGT 
CCAAGGAATT TCTCCCCCAG TGCCTCAGCC CATTTCTCAT TTGCGAGGAG AACGGATGGG 
CGCCGCTGGT CTCTGGCTTC TCTCCCTTCC TCAGGCTATG GGACAAATAC CCCAAGCTCC 
ACCGTCTCCT CATCCTGTTC TTCCCAGGAG AAGTTGCACC AGCTACCATA CCAGCCAACC 
CCAGATGAAT TACACTTCTT ATCCAAACAC TTCTGCACAA CAGAAAGTAT CGCCACTGAG 
AACCGGTGCA GAAACACACC CATGCGTCCA CGTTCCCGGA GTCTCAGCCC TGGACGGTCC 
CCCGCCTGCT GTGACCATGA AATAATTATG ATGAACCATG TCTACAAAGA AAGGTTCCCA 
AAGGCCACAG CTCAGATGGA AGAGCGTCTG AAGGAGATCA TCACCAGCTA CTCTCCAGAC 
CATGTTCTCC CCTTGGCAGA TGGGGTACTT AGTTTCACTC ACCATCAGAT CATTGAGCTG 
GCTCGGGACT GTTTGGATAA ATCTCACCAG GGTCTCATCA CGTCGAGATA CTTTTTTGAG 
TTGCAGCACA AACTGGACAA GTTGCTCCAG GAGGCTCACG ATCGCTCTGA AAGTGGAGAA 
CTGGCATTTA TCAAGCAACT AGTCCGAAAG ATCCTAATTG TCATTGCCCG CCCCGCTCGG 
TTATTGGAGT GTTTGGAATT TGATCCTGAA GAATTTTATT ACCTATTGGA AGCTGCAGAA 
GGCCATGCCA AAGAAGGCCA AGGAATCAAA ACTGACATCC CTAGGTATAT CATCAGCCAG 
CTGGGACTCA ATAAGGACCC TTTAGAAGAA ATGGCTCAGT TGGGGAATTA CGACAGTAGG 
ACAGCAGAGA CACCAGAGAT GGATGAGTCA GTGAGTAGCT CAAATACTTC CCTGAGACTT 
CGAAGGAAAC CCCGAGAGAG TGATTTTGAA ACAATTAAAT TGATCAGCAA TGGAGCCTAC 
GGGGCAGTCT ACTTTGTTCG GCACAAAGAG TCCCGCCAGA GGTTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTCATCCT TCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGAGACATC 
CTGACTTTCG CAGAGAACCC CTTTGTGGTC AGCATGTATT GCTCCTTTGA AACGAGGCGT 
CACTTATGCA TGGTCATGGA GTATGTAGAA GGGGGAGACT GTGCGACCCT AATGAAAAAC 
ATGGGACCTC TCCCAGTTGA TATGGCCAGA ATGTATTTCG CCGAGACCGT CTTGGCCTTG 
GAGTACCTGC ATAATTACGG AATCGTACAC AGGGACTTGA AGCCAGACAA CCTGTTGGTC 
ACCTCCATGG GGCACATAAA ACTGACTGAC TTCGGCTTGT CTAAGGTGGG ATTAATGAGC 
ATGACCACCA ATCTCTATGA AGGCCACATA GAGAAGGACG CTCGAGAGTT CTTAGATAAA 
CAGGTCTGTG GTACACCTGA GTACATTGCC CCCGAGGTGA TTCTGAGACA GGGCTACGGG 
AAACCCGTGG ACTGGTGGGC CATGGGCATT ATCCTCTATG AATTTCTGGT CGGATGTGTG 
CCTTTCTTTG GGGACACTCC AGAAGAGCTA TTTGGACAAG TCATCAGTGA TGAAATCAAC 
TGGCCTGAAA AGGACGAGGC CCCTCCTCCA GACGCTCAGG AGCTGATTAC CTTGCTCCTC 
AGGCAGAATC CGCTGGAGAG GCTGGGAACA GGTGGAGCCT ATGAAGTGAA GCAGCATCGT 
TTCTTCCGCT CCTTAGACTG GAACAGTTTG CTGAGACAGA AGGCGGAATT TATTCCCCAA 
CTGGAATCGG AGGATGACAC AAGTTATTTT GATACTCGGT CAGAGAAGTA TCATCACATG 
GAGACGGAGG AGGAGGACGA TACAAACGAT GAGGACTTCA CCGTGGAGAT AAGGCAGTTT 
TCTTCCTGTT CACACAGGTT TTCAAAAGTT TTCAGCAGTA TAGATCGCAT AACTCAAAAT 
TCAGGAGAAG ACAAAGATGA CTCTGAGGAC AAGACCAAAA GCACAACGTT GCCATCCACA 
GAGACACTCA GCTGGAGTTC CGAATACTCT GAAATGCAAC AGTTATCGAC CTCCAACTCT 
TCAGATACTG AAAGCAACAG GTGCAAACTC AGCTCTGGCT TGCTCCCCAA GCTGGCTATT 
TCGACAGATG GGGAACAAGA TGAGGCTGTC CCTTGCTCTG GAGACCCCAG AGAGGAGCCA 
GAGAAACCTG TCCCTCCCTC TGAGGAGTGT ACTCAGGAGG AGCCCGAGGT CACCACCCCA 
GCCAGCACCA TCAGCAGTTC CACACTGTCA GTTGGCAGTT TTTCAGAGCA CTTGGATCAG 
ATAAATGGGC GAAGCGAGTG TGTGGACAGT ACAGATAATT CCTCAAAGCC ATCCAGTGAA 
CCCACTTCTC ACGTGGCTCG ACAGCGCTTA GAAAGCACAG AGAAAAAGAA AATTTCTGGG 
AAAGTCACAA AGTCCCTCTC GGCCAGTGCT CTGTCCCTCA TGATCCCAGG AGATATGTTC 
GCTGTATCTC CATTGGGAAG CCCAATGTCC CCACACTCCC TGTCTTCAGA CCCTTCTTCT 
TCACGGGATT CCTCTCCCAG CCGAGACTCT TCTGCAGCAT CTGCCAGTCC GCATCAGCCC 
ATTGTCATCC ACAGCTCAGG CAAGAACTAT GGGTTCACCA TCCGTGCTAT CCGCGTGTAC 
GTGGGGGACA GTGACATCTA CACAGTGCAC CATATCGTCT GGAACGTAGA AGAAGGAAGT 
CCCGCATACC AGGCAGGACT GAAGGCCGGG GATCTGATCA CACACATCAA CGGAGAGCCG 
GTGCACGGCC TCGTCCACAC GGAAGTTATC GAGCTCCTGC TGAAGAGTGG GAATAAGGTG 
TCTATCACCA CTACTCCATT TGAAAACACA TCAATCAAAA CGGGACCAGC CAGGAGAAAC 
AGTTACAAGG GCCGGATGGT GAGACGAAGC AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA 
AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC AAGCAGCCTT CTCCTTTGCT CCACACCAGC 
CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG TCATCTGGAG AGAGCCTCCC GGGTTCCCCA 
ACTCACAGCT TGTCCCCGAG GTCTCCAACA CCCAGTTATC GTTCTACTCC CGATTTTCCG 
TCAGGTACAA ATTCCTCCCA GAGCAGCTCC CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA 
GGTTCAGGGC ACATCCGGCC CAGCACCCTC CATGGCCTGG CTCCCAAACT CAGCGGGCAG 
CGATACCGCT CTGGAAGACG GAAGTCGGCT GGCAGCATCC CTCTCTCCCC GCTGGCCAGG 
ACACCCTCTC CCACTCCACA GCCTACCTCT CCTCAGCGTT CACCATCCCC ACTGTTGGGA 
CACTCACTGG GCAATGCCAA GATCACTCAG GCCTTTCCTA GCAAGATGCA CTCTCCCCCC 
ACAATCGTCA GACACATCGT GAGGCCCAAG AGTGCAGAGC CGCCCCGCTC CCCACTGCTG 
AAACGGGTGC AGTCGGAGGA AAAGTTGTCA CCCTCCTATG GCAGTGACAA GAAGCTTCTG 
TGCTCCCGCA AGCATAGCCT AGAGGTGACA CAAGAGGAGG TACAGAGGGA GCAGTGTCAG 
CGGGAAGTGA CACTGCAGAG CCTGGAAGAG AATGTGTGTG ACGCTCCTTC CCTCAGTCGG 
GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA 
GAGTCTGTGG ATGACCTGGA CCGGGACAAG CTGAAAGCCA AGGTTGTCGT AAAGAAACCA 
GAAGAGAAAC ATGAATCGCA CCAGAAACCT CACAGCCTTG GTGGTGATTC GGAAAGCTAT 
GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA AAAGTGTACT CCAAGGGGTT GGAAAGGTCA 
GGCCATTTTG AAAACACATC AGCAGAGTTG CCTTCTGTGG GCAGCCTGCT GAAGGACACT 
CTTCACAAGC AGGCCAGCGT GAGGGCCAGC GAGGGGGTGA CCTCAGACGG GGCAGCTTGC 
AGCCTGACAC CAGGGGAGCA CAGCCAGTCT CTAGGTGACT TTAAGCGGGC CTCAGCTTCT 
GGCATTCTCC ATGATAGTGT GTGCCCCATC TCTGATAGGC CTGCTCCTGG AAAGGTTGAA 
TACTCGGAGA AGGCCTCTCA GGCCAAAGAG CTCCTTCGAA GTGAAAAACT AGACAGCAAG 
CTGGCCAATA TTGATTACCT CAGAAAGAAA ATGTCATTGG ATGACAAAGA TGACAGCCAC 
TGTGCCATCC TGAAACCCAA GATAACATCT AGCGCCCATG AATGTCTGCC AGGGAACCCC 
ATACGGCCCA TGGCAGGGCA ACAAGAGACC CCGCCAGCCT CTGAAAACCG GGCATTCATC 
AACAGTACCC ACACACCTCA GATGAGTGCA GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT 
GGCCGGGTAG AGAACGGAGG GGAGAAAGCA GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG 
AAGAGCCCCT CCGAGTATAA GCTAGAGGGC AGGTCAGTTT CATGTCTCAA GCCGATCGAG 
GGCACACTGG ACATTGCTCT CCTGTCTGGA CCTCACGCCT CCAAAACAGA GTTGCTTTCC 
CCAGAGCCTG CACAGAGTCC CAGCCCAGGC ATCAACGTGG GACCATGTGT GCCACTAGCT 
CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC TCCACCAGCC TGAGAGAGCC TTCCTCAGCC 
AACTTAAAAG TAAATAAATC TTATCTGCTG GAGCCTCGGT TCCTACCCCC GAGCCGGGCT 
CTCCAGGACT CTCTCGCAGC CTCTGGGCCA GAACCGAAGT CAAAGCCGGA AAGGAAGCTC 
ATTCATCCTT CTGCCAGGAG CCCAGCAACT GTCACAGAGA GCAATCTTCA GCAGAAAGAG 
GGTGGTCCCG CCACACACCA AGACCGCTCC ACTGACACCA GGAACCTCCC TGGCCCAGGG 
CAGACCCTAC ACAATGTGGA CCTACCCAGG CTGTGTACAC GTGCCCCACT CCCACCGGAA 
GGGACGCCCG CAAAGGAGAA GCCATGTCTG AAGGAACCCT CTGCCAAGGT GAAAAGCGAG 
TGGTCTGCCG TGAGGGATGA CGGACACAGA GATCCCTGTG CGAAGCTGTG CCCGGCAGAG 
ACTGGTAAAG CCAGCGACAG TTCCAAACCC CTGCCTTCCG GGGGGAGGAC CCAACCCGAT 
TTCTACAAGC AGACCCAGAC TTCGGAGAAA GCATGGGCGC ATGCAAAAAC AAACCACAAA 
GATAGCCAAG ATGAGGTGAA GTCCCTGGCC AGGGAGGACT CAGCTTCACT TTTATATGAA 
AAGGAGATAG GCCGGGCACG AAAAGGTCCT GAACCCAAAC CGGAAGTTCC TGCTACCCGG 
TGCCCTCCTC AGCCACCAGG AATTGAGGGT GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC 
TCTTTGCAGA AACAGGCTCC CAAAGAGCCA GACAGGAAGG AACAGACTTC GCAAAGGCCT 
GGAGGTAGTG GCCCTCAACA ACCCCCACCC ACCAAAGAGC TGTCTAACTC AGCATCCTGG 
CAGCACGGCA GTTCTCCGAG TCACACTTTA AAGAAGGAGC CCGGGACCAA AGCTGCCGCT 
GCAGAACCAA GCACCAGCCT TCATGACACT CCCCGATCTG CTACAGCCAC CACCACTGCC 
ATTGCCACCA CCACCACTAC CACCAGTGCC GGGCACAGTG ACTGCAGTAG CCATAAGGCC 
CGGCCTGGCC CTGACCCCAG CCCTTCAAAG TCTAAGCACC AAGACAGGTC CCTCTCCTCA 
CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC AAAGAGCCTG TCACTCAACC CCTGGGTGGT 
TCCATCAGAG AAGGCAAGGG TGGCAGCAAG GGTCCAGTGG ACACATTTTC TGCTGTCCTG 
ACCACCCAGG GCAAAGCAAG TGATGTGCTT GTGCAGGGAG AAGGTCGGGT CTCAATCATT 
GTCCACACTG AAGAGTGTCC TCTCGATGCC AAACTGAAAA ACACCAATGG AGGGTGTCCC 
CCAGAGATGC AGGCGAAGCA TCCACCCAGA CAAGGACATC TCAGTGAAGC AGCAGACCAG 
AAGCCACTCA TTGCTGGTGA GAAGCAAAGC CCGTCTCCAA AGCATCCCAA ACCATCCACT 
GTGAAAGATT ACCCCAGTCT GTGCAGACAG ACAGACAGAA GCCCAAGCCA TCAGGCTACC 
ACTGGGGACA GGAAGGCAGA AGGAAAGAAA TGCACAGACG CACTTTATGT CGCAGCCCCA 
GAGGGCTACA AGCCAGAGGC CAGCCCTTCT CTCCACCACG GCGAGACCGG ACTCAGAGGC 
TCAGAGAGGC CACCCATGGG CATGGGGAAG GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA 
GGTCCCCAGA AGTCACTGGC TGAAACAGGC AAGCCCAGCG GTATGAAAAG GTCACCCTCT 
GCCACCGTGC AGAGCTCTCT CCGCTCAGCT GCCCCCCCAG AAAAGTCTCT GAGTTACTCA 
GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC 
TCATCTGCCA AGGCTACAGG GGGGACCTCA GAGTTCCCAG CCCCCAGCAG CAGGGACCAC 
AGGAAGCTTC AGTCTGGAGG AGACGGCCGA AGCCAAATGA TAAAGAGTGA CTCTCTGCCC 
TCCTTCCGCC TCTCCACCTC TGCTCTGGAG TCACATTTCC AGGATCCACA GGTGCCCATC 
GCATCAGGCC ACCGAGGCAG GGCACTGTCA GTAACTGCTG CCACAGGAGA ACCCAAAGGG 
AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG AAACAGAATG CGTGCAGAGA GGCGACCAGA 
GCACCCCCAG CCCCAAGCAC AGATCGCTCC CTCCCTCTTT CCTCAGAGAA AGACTTCGTG 
GTTCGGCAGA GAAGGGGCAA GGAGACCTTA AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517768.1
CDS	106..7365
Translation	MDESSILRRRGLQKELSLPRRGSLCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_006517705.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517705.4

ORF Insert Sequence:

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGA AGGAGCTGAG CCTCCCCCGA 
AGAGGAAGTC TTTGCCGAAC AAGCAACCGG AAGAGTTTGA TAGGCAATGG CCAGTCTCCA 
GCACTGCCTC GACCACACTC ACCTCTCTCT GCTCATGCAG GAAATAGCCC TCAAGACAGT 
CCAAGGAATT TCTCCCCCAG TGCCTCAGCC CATTTCTCAT TTGCGAGGAG AACGGATGGG 
CGCCGCTGGT CTCTGGCTTC TCTCCCTTCC TCAGGCTATG GGACAAATAC CCCAAGCTCC 
ACCGTCTCCT CATCCTGTTC TTCCCAGGAG AAGTTGCACC AGCTACCATA CCAGCCAACC 
CCAGATGAAT TACACTTCTT ATCCAAACAC TTCTGCACAA CAGAAAGTAT CGCCACTGAG 
AACCGGTGCA GAAACACACC CATGCGTCCA CGTTCCCGGA GTCTCAGCCC TGGACGGTCC 
CCCGCCTGCT GTGACCATGA AATAATTATG ATGAACCATG TCTACAAAGA AAGGTTCCCA 
AAGGCCACAG CTCAGATGGA AGAGCGTCTG AAGGAGATCA TCACCAGCTA CTCTCCAGAC 
CATGTTCTCC CCTTGGCAGA TGGGGTACTT AGTTTCACTC ACCATCAGAT CATTGAGCTG 
GCTCGGGACT GTTTGGATAA ATCTCACCAG GGTCTCATCA CGTCGAGATA CTTTTTTGAG 
TTGCAGCACA AACTGGACAA GTTGCTCCAG GAGGCTCACG ATCGCTCTGA AAGTGGAGAA 
CTGGCATTTA TCAAGCAACT AGTCCGAAAG ATCCTAATTG TCATTGCCCG CCCCGCTCGG 
TTATTGGAGT GTTTGGAATT TGATCCTGAA GAATTTTATT ACCTATTGGA AGCTGCAGAA 
GGCCATGCCA AAGAAGGCCA AGGAATCAAA ACTGACATCC CTAGGTATAT CATCAGCCAG 
CTGGGACTCA ATAAGGACCC TTTAGAAGAA ATGGCTCAGT TGGGGAATTA CGACAGTAGG 
ACAGCAGAGA CACCAGAGAT GGATGAGTCA GTGAGTAGCT CAAATACTTC CCTGAGACTT 
CGAAGGAAAC CCCGAGAGAG TGATTTTGAA ACAATTAAAT TGATCAGCAA TGGAGCCTAC 
GGGGCAGTCT ACTTTGTTCG GCACAAAGAG TCCCGCCAGA GGTTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTCATCCT TCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGAGACATC 
CTGACTTTCG CAGAGAACCC CTTTGTGGTC AGCATGTATT GCTCCTTTGA AACGAGGCGT 
CACTTATGCA TGGTCATGGA GTATGTAGAA GGGGGAGACT GTGCGACCCT AATGAAAAAC 
ATGGGACCTC TCCCAGTTGA TATGGCCAGA ATGTATTTCG CCGAGACCGT CTTGGCCTTG 
GAGTACCTGC ATAATTACGG AATCGTACAC AGGGACTTGA AGCCAGACAA CCTGTTGGTC 
ACCTCCATGG GGCACATAAA ACTGACTGAC TTCGGCTTGT CTAAGGTGGG ATTAATGAGC 
ATGACCACCA ATCTCTATGA AGGCCACATA GAGAAGGACG CTCGAGAGTT CTTAGATAAA 
CAGGTCTGTG GTACACCTGA GTACATTGCC CCCGAGGTGA TTCTGAGACA GGGCTACGGG 
AAACCCGTGG ACTGGTGGGC CATGGGCATT ATCCTCTATG AATTTCTGGT CGGATGTGTG 
CCTTTCTTTG GGGACACTCC AGAAGAGCTA TTTGGACAAG TCATCAGTGA TGAAATCAAC 
TGGCCTGAAA AGGACGAGGC CCCTCCTCCA GACGCTCAGG AGCTGATTAC CTTGCTCCTC 
AGGCAGAATC CGCTGGAGAG GCTGGGAACA GGTGGAGCCT ATGAAGTGAA GCAGCATCGT 
TTCTTCCGCT CCTTAGACTG GAACAGTTTG CTGAGACAGA AGGCGGAATT TATTCCCCAA 
CTGGAATCGG AGGATGACAC AAGTTATTTT GATACTCGGT CAGAGAAGTA TCATCACATG 
GAGACGGAGG AGGAGGACGA TACAAACGAT GAGGACTTCA CCGTGGAGAT AAGGCAGTTT 
TCTTCCTGTT CACACAGGTT TTCAAAAGTT TTCAGCAGTA TAGATCGCAT AACTCAAAAT 
TCAGGAGAAG ACAAAGATGA CTCTGAGGAC AAGACCAAAA GCACAACGTT GCCATCCACA 
GAGACACTCA GCTGGAGTTC CGAATACTCT GAAATGCAAC AGTTATCGAC CTCCAACTCT 
TCAGATACTG AAAGCAACAG GTGCAAACTC AGCTCTGGCT TGCTCCCCAA GCTGGCTATT 
TCGACAGATG GGGAACAAGA TGAGGCTGTC CCTTGCTCTG GAGACCCCAG AGAGGAGCCA 
GAGAAACCTG TCCCTCCCTC TGAGGAGTGT ACTCAGGAGG AGCCCGAGGT CACCACCCCA 
GCCAGCACCA TCAGCAGTTC CACACTGTCA GTTGGCAGTT TTTCAGAGCA CTTGGATCAG 
ATAAATGGGC GAAGCGAGTG TGTGGACAGT ACAGATAATT CCTCAAAGCC ATCCAGTGAA 
CCCACTTCTC ACGTGGCTCG ACAGCGCTTA GAAAGCACAG AGAAAAAGAA AATTTCTGGG 
AAAGTCACAA AGTCCCTCTC GGCCAGTGCT CTGTCCCTCA TGATCCCAGG AGATATGTTC 
GCTGTATCTC CATTGGGAAG CCCAATGTCC CCACACTCCC TGTCTTCAGA CCCTTCTTCT 
TCACGGGATT CCTCTCCCAG CCGAGACTCT TCTGCAGCAT CTGCCAGTCC GCATCAGCCC 
ATTGTCATCC ACAGCTCAGG CAAGAACTAT GGGTTCACCA TCCGTGCTAT CCGCGTGTAC 
GTGGGGGACA GTGACATCTA CACAGTGCAC CATATCGTCT GGAACGTAGA AGAAGGAAGT 
CCCGCATACC AGGCAGGACT GAAGGCCGGG GATCTGATCA CACACATCAA CGGAGAGCCG 
GTGCACGGCC TCGTCCACAC GGAAGTTATC GAGCTCCTGC TGAAGAGTGG GAATAAGGTG 
TCTATCACCA CTACTCCATT TGAAAACACA TCAATCAAAA CGGGACCAGC CAGGAGAAAC 
AGTTACAAGG GCCGGATGGT GAGACGAAGC AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA 
AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC AAGCAGCCTT CTCCTTTGCT CCACACCAGC 
CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG TCATCTGGAG AGAGCCTCCC GGGTTCCCCA 
ACTCACAGCT TGTCCCCGAG GTCTCCAACA CCCAGTTATC GTTCTACTCC CGATTTTCCG 
TCAGGTACAA ATTCCTCCCA GAGCAGCTCC CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA 
GGTTCAGGGC ACATCCGGCC CAGCACCCTC CATGGCCTGG CTCCCAAACT CAGCGGGCAG 
CGATACCGCT CTGGAAGACG GAAGTCGGCT GGCAGCATCC CTCTCTCCCC GCTGGCCAGG 
ACACCCTCTC CCACTCCACA GCCTACCTCT CCTCAGCGTT CACCATCCCC ACTGTTGGGA 
CACTCACTGG GCAATGCCAA GATCACTCAG GCCTTTCCTA GCAAGATGCA CTCTCCCCCC 
ACAATCGTCA GACACATCGT GAGGCCCAAG AGTGCAGAGC CGCCCCGCTC CCCACTGCTG 
AAACGGGTGC AGTCGGAGGA AAAGTTGTCA CCCTCCTATG GCAGTGACAA GAAGCTTCTG 
TGCTCCCGCA AGCATAGCCT AGAGGTGACA CAAGAGGAGG TACAGAGGGA GCAGTGTCAG 
CGGGAAGTGA CACTGCAGAG CCTGGAAGAG AATGTGTGTG ACGCTCCTTC CCTCAGTCGG 
GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA 
GAGTCTGTGG ATGACCTGGA CCGGGACAAG CTGAAAGCCA AGGTTGTCGT AAAGAAACCA 
GAAGAGAAAC ATGAATCGCA CCAGAAACCT CACAGCCTTG GTGGTGATTC GGAAAGCTAT 
GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA AAAGTGTACT CCAAGGGGTT GGAAAGGTCA 
GGCCATTTTG AAAACACATC AGCAGAGTTG CCTTCTGTGG GCAGCCTGCT GAAGGACACT 
CTTCACAAGC AGGCCAGCGT GAGGGCCAGC GAGGGGGTGA CCTCAGACGG GGCAGCTTGC 
AGCCTGACAC CAGGGGAGCA CAGCCAGTCT CTAGGTGACT TTAAGCGGGC CTCAGCTTCT 
GGCATTCTCC ATGATAGTGT GTGCCCCATC TCTGATAGGC CTGCTCCTGG AAAGGTTGAA 
TACTCGGAGA AGGCCTCTCA GGCCAAAGAG CTCCTTCGAA GTGAAAAACT AGACAGCAAG 
CTGGCCAATA TTGATTACCT CAGAAAGAAA ATGTCATTGG ATGACAAAGA TGACAGCCAC 
TGTGCCATCC TGAAACCCAA GATAACATCT AGCGCCCATG AATGTCTGCC AGGGAACCCC 
ATACGGCCCA TGGCAGGGCA ACAAGAGACC CCGCCAGCCT CTGAAAACCG GGCATTCATC 
AACAGTACCC ACACACCTCA GATGAGTGCA GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT 
GGCCGGGTAG AGAACGGAGG GGAGAAAGCA GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG 
AAGAGCCCCT CCGAGTATAA GCTAGAGGGC AGGTCAGTTT CATGTCTCAA GCCGATCGAG 
GGCACACTGG ACATTGCTCT CCTGTCTGGA CCTCACGCCT CCAAAACAGA GTTGCTTTCC 
CCAGAGCCTG CACAGAGTCC CAGCCCAGGC ATCAACGTGG GACCATGTGT GCCACTAGCT 
CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC TCCACCAGCC TGAGAGAGCC TTCCTCAGCC 
AACTTAAAAG TAAATAAATC TTATCTGCTG GAGCCTCGGT TCCTACCCCC GAGCCGGGCT 
CTCCAGGACT CTCTCGCAGC CTCTGGGCCA GAACCGAAGT CAAAGCCGGA AAGGAAGCTC 
ATTCATCCTT CTGCCAGGAG CCCAGCAACT GTCACAGAGA GCAATCTTCA GCAGAAAGAG 
GGTGGTCCCG CCACACACCA AGACCGCTCC ACTGACACCA GGAACCTCCC TGGCCCAGGG 
CAGACCCTAC ACAATGTGGA CCTACCCAGG CTGTGTACAC GTGCCCCACT CCCACCGGAA 
GGGACGCCCG CAAAGGAGAA GCCATGTCTG AAGGAACCCT CTGCCAAGGT GAAAAGCGAG 
TGGTCTGCCG TGAGGGATGA CGGACACAGA GATCCCTGTG CGAAGCTGTG CCCGGCAGAG 
ACTGGTAAAG CCAGCGACAG TTCCAAACCC CTGCCTTCCG GGGGGAGGAC CCAACCCGAT 
TTCTACAAGC AGACCCAGAC TTCGGAGAAA GCATGGGCGC ATGCAAAAAC AAACCACAAA 
GATAGCCAAG ATGAGGTGAA GTCCCTGGCC AGGGAGGACT CAGCTTCACT TTTATATGAA 
AAGGAGATAG GCCGGGCACG AAAAGGTCCT GAACCCAAAC CGGAAGTTCC TGCTACCCGG 
TGCCCTCCTC AGCCACCAGG AATTGAGGGT GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC 
TCTTTGCAGA AACAGGCTCC CAAAGAGCCA GACAGGAAGG AACAGACTTC GCAAAGGCCT 
GGAGGTAGTG GCCCTCAACA ACCCCCACCC ACCAAAGAGC TGTCTAACTC AGCATCCTGG 
CAGCACGGCA GTTCTCCGAG TCACACTTTA AAGAAGGAGC CCGGGACCAA AGCTGCCGCT 
GCAGAACCAA GCACCAGCCT TCATGACACT CCCCGATCTG CTACAGCCAC CACCACTGCC 
ATTGCCACCA CCACCACTAC CACCAGTGCC GGGCACAGTG ACTGCAGTAG CCATAAGGCC 
CGGCCTGGCC CTGACCCCAG CCCTTCAAAG TCTAAGCACC AAGACAGGTC CCTCTCCTCA 
CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC AAAGAGCCTG TCACTCAACC CCTGGGTGGT 
TCCATCAGAG AAGGCAAGGG TGGCAGCAAG GGTCCAGTGG ACACATTTTC TGCTGTCCTG 
ACCACCCAGG GCAAAGCAAG TGATGTGCTT GTGCAGGGAG AAGGTCGGGT CTCAATCATT 
GTCCACACTG AAGAGTGTCC TCTCGATGCC AAACTGAAAA ACACCAATGG AGGGTGTCCC 
CCAGAGATGC AGGCGAAGCA TCCACCCAGA CAAGGACATC TCAGTGAAGC AGCAGACCAG 
AAGCCACTCA TTGCTGGTGA GAAGCAAAGC CCGTCTCCAA AGCATCCCAA ACCATCCACT 
GTGAAAGATT ACCCCAGTCT GTGCAGACAG ACAGACAGAA GCCCAAGCCA TCAGGCTACC 
ACTGGGGACA GGAAGGCAGA AGGAAAGAAA TGCACAGACG CACTTTATGT CGCAGCCCCA 
GAGGGCTACA AGCCAGAGGC CAGCCCTTCT CTCCACCACG GCGAGACCGG ACTCAGAGGC 
TCAGAGAGGC CACCCATGGG CATGGGGAAG GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA 
GGTCCCCAGA AGTCACTGGC TGAAACAGGC AAGCCCAGCG GTATGAAAAG GTCACCCTCT 
GCCACCGTGC AGAGCTCTCT CCGCTCAGCT GCCCCCCCAG AAAAGTCTCT GAGTTACTCA 
GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC 
TCATCTGCCA AGGCTACAGG GGGGACCTCA GAGTTCCCAG CCCCCAGCAG CAGGGACCAC 
AGGAAGCTTC AGTCTGGAGG AGACGGCCGA AGCCAAATGA TAAAGAGTGA CTCTCTGCCC 
TCCTTCCGCC TCTCCACCTC TGCTCTGGAG TCACATTTCC AGGATCCACA GGTGCCCATC 
GCATCAGGCC ACCGAGGCAG GGCACTGTCA GTAACTGCTG CCACAGGAGA ACCCAAAGGG 
AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG AAACAGAATG CGTGCAGAGA GGCGACCAGA 
GCACCCCCAG CCCCAAGCAC AGATCGCTCC CTCCCTCTTT CCTCAGAGAA AGACTTCGTG 
GTTCGGCAGA GAAGGGGCAA GGAGACCTTA AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_011244673.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242975.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X14, mRNA.

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CloneID	OMu74761
Clone ID Related Accession (Same CDS sequence)	XM_011244673.2 , XM_011244673.3
Accession Version	XM_011244673.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244673.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATGT CTGACCCCAA TTTCTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGCACACAA AGTTGCCGAA CAAGCAACCG GAAGAGTTTG 
ATAGGCAATG GCCAGTCTCC AGCACTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGACAG TCCAAGGAAT TTCTCCCCCA GTGCCTCAGC CCATTTCTCA 
TTTGCGAGGA GAACGGATGG GCGCCGCTGG TCTCTGGCTT CTCTCCCTTC CTCAGGCTAT 
GGGACAAATA CCCCAAGCTC CACCGTCTCC TCATCCTGTT CTTCCCAGGA GAAGTTGCAC 
CAGCTACCAT ACCAGCCAAC CCCAGATGAA TTACACTTCT TATCCAAACA CTTCTGCACA 
ACAGAAAGTA TCGCCACTGA GAACCGGTGC AGAAACACAC CCATGCGTCC ACGTTCCCGG 
AGTCTCAGCC CTGGACGGTC CCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCCACA GCTCAGATGG AAGAGCGTCT GAAGGAGATC 
ATCACCAGCT ACTCTCCAGA CCATGTTCTC CCCTTGGCAG ATGGGGTACT TAGTTTCACT 
CACCATCAGA TCATTGAGCT GGCTCGGGAC TGTTTGGATA AATCTCACCA GGGTCTCATC 
ACGTCGAGAT ACTTTTTTGA GTTGCAGCAC AAACTGGACA AGTTGCTCCA GGAGGCTCAC 
GATCGCTCTG AAAGTGGAGA ACTGGCATTT ATCAAGCAAC TAGTCCGAAA GATCCTAATT 
GTCATTGCCC GCCCCGCTCG GTTATTGGAG TGTTTGGAAT TTGATCCTGA AGAATTTTAT 
TACCTATTGG AAGCTGCAGA AGGCCATGCC AAAGAAGGCC AAGGAATCAA AACTGACATC 
CCTAGGTATA TCATCAGCCA GCTGGGACTC AATAAGGACC CTTTAGAAGA AATGGCTCAG 
TTGGGGAATT ACGACAGTAG GACAGCAGAG ACACCAGAGA TGGATGAGTC AGTGAGTAGC 
TCAAATACTT CCCTGAGACT TCGAAGGAAA CCCCGAGAGA GTGATTTTGA AACAATTAAA 
TTGATCAGCA ATGGAGCCTA CGGGGCAGTC TACTTTGTTC GGCACAAAGA GTCCCGCCAG 
AGGTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTCATCC TTCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGAGACAT CCTGACTTTC GCAGAGAACC CCTTTGTGGT CAGCATGTAT 
TGCTCCTTTG AAACGAGGCG TCACTTATGC ATGGTCATGG AGTATGTAGA AGGGGGAGAC 
TGTGCGACCC TAATGAAAAA CATGGGACCT CTCCCAGTTG ATATGGCCAG AATGTATTTC 
GCCGAGACCG TCTTGGCCTT GGAGTACCTG CATAATTACG GAATCGTACA CAGGGACTTG 
AAGCCAGACA ACCTGTTGGT CACCTCCATG GGGCACATAA AACTGACTGA CTTCGGCTTG 
TCTAAGGTGG GATTAATGAG CATGACCACC AATCTCTATG AAGGCCACAT AGAGAAGGAC 
GCTCGAGAGT TCTTAGATAA ACAGGTCTGT GGTACACCTG AGTACATTGC CCCCGAGGTG 
ATTCTGAGAC AGGGCTACGG GAAACCCGTG GACTGGTGGG CCATGGGCAT TATCCTCTAT 
GAATTTCTGG TCGGATGTGT GCCTTTCTTT GGGGACACTC CAGAAGAGCT ATTTGGACAA 
GTCATCAGTG ATGAAATCAA CTGGCCTGAA AAGGACGAGG CCCCTCCTCC AGACGCTCAG 
GAGCTGATTA CCTTGCTCCT CAGGCAGAAT CCGCTGGAGA GGCTGGGAAC AGGTGGAGCC 
TATGAAGTGA AGCAGCATCG TTTCTTCCGC TCCTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCGGAAT TTATTCCCCA ACTGGAATCG GAGGATGACA CAAGTTATTT TGATACTCGG 
TCAGAGAAGT ATCATCACAT GGAGACGGAG GAGGAGGACG ATACAAACGA TGAGGACTTC 
ACCGTGGAGA TAAGGCAGTT TTCTTCCTGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGCA TAACTCAAAA TTCAGGAGAA GACAAAGATG ACTCTGAGGA CAAGACCAAA 
AGCACAACGT TGCCATCCAC AGAGACACTC AGCTGGAGTT CCGAATACTC TGAAATGCAA 
CAGTTATCGA CCTCCAACTC TTCAGATACT GAAAGCAACA GGTGCAAACT CAGCTCTGGC 
TTGCTCCCCA AGCTGGCTAT TTCGACAGAT GGGGAACAAG ATGAGGCTGT CCCTTGCTCT 
GGAGACCCCA GAGAGGAGCC AGAGAAACCT GTCCCTCCCT CTGAGGAGTG TACTCAGGAG 
GAGCCCGAGG TCACCACCCC AGCCAGCACC ATCAGCAGTT CCACACTGTC AGTTGGCAGT 
TTTTCAGAGC ACTTGGATCA GATAAATGGG CGAAGCGAGT GTGTGGACAG TACAGATAAT 
TCCTCAAAGC CATCCAGTGA ACCCACTTCT CACGTGGCTC GACAGCGCTT AGAAAGCACA 
GAGAAAAAGA AAATTTCTGG GAAAGTCACA AAGTCCCTCT CGGCCAGTGC TCTGTCCCTC 
ATGATCCCAG GAGATATGTT CGCTGTATCT CCATTGGGAA GCCCAATGTC CCCACACTCC 
CTGTCTTCAG ACCCTTCTTC TTCACGGGAT TCCTCTCCCA GCCGAGACTC TTCTGCAGCA 
TCTGCCAGTC CGCATCAGCC CATTGTCATC CACAGCTCAG GCAAGAACTA TGGGTTCACC 
ATCCGTGCTA TCCGCGTGTA CGTGGGGGAC AGTGACATCT ACACAGTGCA CCATATCGTC 
TGGAACGTAG AAGAAGGAAG TCCCGCATAC CAGGCAGGAC TGAAGGCCGG GGATCTGATC 
ACACACATCA ACGGAGAGCC GGTGCACGGC CTCGTCCACA CGGAAGTTAT CGAGCTCCTG 
CTGAAGAGTG GGAATAAGGT GTCTATCACC ACTACTCCAT TTGAAAACAC ATCAATCAAA 
ACGGGACCAG CCAGGAGAAA CAGTTACAAG GGCCGGATGG TGAGACGAAG CAAGAAGTCC 
AAGAAGAAGG AGAGTCTAGA AAGGAGGAGA TCTCTCTTCA AGAAGCTGGC CAAGCAGCCT 
TCTCCTTTGC TCCACACCAG CCGAAGTTTC TCCTGCTTAA ACCGGTCCCT GTCATCTGGA 
GAGAGCCTCC CGGGTTCCCC AACTCACAGC TTGTCCCCGA GGTCTCCAAC ACCCAGTTAT 
CGTTCTACTC CCGATTTTCC GTCAGGTACA AATTCCTCCC AGAGCAGCTC CCCAAGTTCA 
AGTGCCCCCA ATTCTCCAGC AGGTTCAGGG CACATCCGGC CCAGCACCCT CCATGGCCTG 
GCTCCCAAAC TCAGCGGGCA GCGATACCGC TCTGGAAGAC GGAAGTCGGC TGGCAGCATC 
CCTCTCTCCC CGCTGGCCAG GACACCCTCT CCCACTCCAC AGCCTACCTC TCCTCAGCGT 
TCACCATCCC CACTGTTGGG ACACTCACTG GGCAATGCCA AGATCACTCA GGCCTTTCCT 
AGCAAGATGC ACTCTCCCCC CACAATCGTC AGACACATCG TGAGGCCCAA GAGTGCAGAG 
CCGCCCCGCT CCCCACTGCT GAAACGGGTG CAGTCGGAGG AAAAGTTGTC ACCCTCCTAT 
GGCAGTGACA AGAAGCTTCT GTGCTCCCGC AAGCATAGCC TAGAGGTGAC ACAAGAGGAG 
GTACAGAGGG AGCAGTGTCA GCGGGAAGTG ACACTGCAGA GCCTGGAAGA GAATGTGTGT 
GACGCTCCTT CCCTCAGTCG GGCCAGGCCA GTGGAGCAAG GCTGTCTGAA ACGCCCCGTG 
TCCCGGAAGG TGGGCAGGCA AGAGTCTGTG GATGACCTGG ACCGGGACAA GCTGAAAGCC 
AAGGTTGTCG TAAAGAAACC AGAAGAGAAA CATGAATCGC ACCAGAAACC TCACAGCCTT 
GGTGGTGATT CGGAAAGCTA TGCTCTCTTC AGGCTAGAGG AGAGAGAGAA AAAAGTGTAC 
TCCAAGGGGT TGGAAAGGTC AGGCCATTTT GAAAACACAT CAGCAGAGTT GCCTTCTGTG 
GGCAGCCTGC TGAAGGACAC TCTTCACAAG CAGGCCAGCG TGAGGGCCAG CGAGGGGGTG 
ACCTCAGACG GGGCAGCTTG CAGCCTGACA CCAGGGGAGC ACAGCCAGTC TCTAGGTGAC 
TTTAAGCGGG CCTCAGCTTC TGGCATTCTC CATGATAGTG TGTGCCCCAT CTCTGATAGG 
CCTGCTCCTG GAAAGGTTGA ATACTCGGAG AAGGCCTCTC AGGCCAAAGA GCTCCTTCGA 
AGTGAAAAAC TAGACAGCAA GCTGGCCAAT ATTGATTACC TCAGAAAGAA AATGTCATTG 
GATGACAAAG ATGACAGCCA CTGTGCCATC CTGAAACCCA AGATAACATC TAGCGCCCAT 
GAATGTCTGC CAGGGAACCC CATACGGCCC ATGGCAGGGC AACAAGAGAC CCCGCCAGCC 
TCTGAAAACC GGGCATTCAT CAACAGTACC CACACACCTC AGATGAGTGC AGTTTCCTTT 
GTTCCTCTCA AAGCCTTAGC TGGCCGGGTA GAGAACGGAG GGGAGAAAGC AGGCTTAGCT 
GCTCCCGAGT CCCCTGTCAG GAAGAGCCCC TCCGAGTATA AGCTAGAGGG CAGGTCAGTT 
TCATGTCTCA AGCCGATCGA GGGCACACTG GACATTGCTC TCCTGTCTGG ACCTCACGCC 
TCCAAAACAG AGTTGCTTTC CCCAGAGCCT GCACAGAGTC CCAGCCCAGG CATCAACGTG 
GGACCATGTG TGCCACTAGC TCTTCCTGGG AGCAGTGGGA AAAAGGGAGA CTCCACCAGC 
CTGAGAGAGC CTTCCTCAGC CAACTTAAAA GTAAATAAAT CTTATCTGCT GGAGCCTCGG 
TTCCTACCCC CGAGCCGGGC TCTCCAGGAC TCTCTCGCAG CCTCTGGGCC AGAACCGAAG 
TCAAAGCCGG AAAGGAAGCT CATTCATCCT TCTGCCAGGA GCCCAGCAAC TGTCACAGAG 
AGCAATCTTC AGCAGAAAGA GGGTGGTCCC GCCACACACC AAGACCGCTC CACTGACACC 
AGGAACCTCC CTGGCCCAGG GCAGACCCTA CACAATGTGG ACCTACCCAG GCTGTGTACA 
CGTGCCCCAC TCCCACCGGA AGGGACGCCC GCAAAGGAGA AGCCATGTCT GAAGGAACCC 
TCTGCCAAGG TGAAAAGCGA GTGGTCTGCC GTGAGGGATG ACGGACACAG AGATCCCTGT 
GCGAAGCTGT GCCCGGCAGA GACTGGTAAA GCCAGCGACA GTTCCAAACC CCTGCCTTCC 
GGGGGGAGGA CCCAACCCGA TTTCTACAAG CAGACCCAGA CTTCGGAGAA AGCATGGGCG 
CATGCAAAAA CAAACCACAA AGATAGCCAA GATGAGGTGA AGTCCCTGGC CAGGGAGGAC 
TCAGCTTCAC TTTTATATGA AAAGGAGATA GGCCGGGCAC GAAAAGGTCC TGAACCCAAA 
CCGGAAGTTC CTGCTACCCG GTGCCCTCCT CAGCCACCAG GAATTGAGGG TGAGAAGCGA 
GAAAAGCTCT CCGCTGCCCC CTCTTTGCAG AAACAGGCTC CCAAAGAGCC AGACAGGAAG 
GAACAGACTT CGCAAAGGCC TGGAGGTAGT GGCCCTCAAC AACCCCCACC CACCAAAGAG 
CTGTCTAACT CAGCATCCTG GCAGCACGGC AGTTCTCCGA GTCACACTTT AAAGAAGGAG 
CCCGGGACCA AAGCTGCCGC TGCAGAACCA AGCACCAGCC TTCATGACAC TCCCCGATCT 
GCTACAGCCA CCACCACTGC CATTGCCACC ACCACCACTA CCACCAGTGC CGGGCACAGT 
GACTGCAGTA GCCATAAGGC CCGGCCTGGC CCTGACCCCA GCCCTTCAAA GTCTAAGCAC 
CAAGACAGGT CCCTCTCCTC ACAGAAGCTG AGTGCTGGCT CTGCAAAAGG CAAAGAGCCT 
GTCACTCAAC CCCTGGGTGG TTCCATCAGA GAAGGCAAGG GTGGCAGCAA GGGTCCAGTG 
GACACATTTT CTGCTGTCCT GACCACCCAG GGCAAAGCAA GTGATGTGCT TGTGCAGGGA 
GAAGGTCGGG TCTCAATCAT TGTCCACACT GAAGAGTGTC CTCTCGATGC CAAACTGAAA 
AACACCAATG GAGGGTGTCC CCCAGAGATG CAGGCGAAGC ATCCACCCAG ACAAGGACAT 
CTCAGTGAAG CAGCAGACCA GAAGCCACTC ATTGCTGGTG AGAAGCAAAG CCCGTCTCCA 
AAGCATCCCA AACCATCCAC TGTGAAAGAT TACCCCAGTC TGTGCAGACA GACAGACAGA 
AGCCCAAGCC ATCAGGCTAC CACTGGGGAC AGGAAGGCAG AAGGAAAGAA ATGCACAGAC 
GCACTTTATG TCGCAGCCCC AGAGGGCTAC AAGCCAGAGG CCAGCCCTTC TCTCCACCAC 
GGCGAGACCG GACTCAGAGG CTCAGAGAGG CCACCCATGG GCATGGGGAA GGGCTTCTCT 
GAGCCCAAGG GGAAAGGGCC AGGTCCCCAG AAGTCACTGG CTGAAACAGG CAAGCCCAGC 
GGTATGAAAA GGTCACCCTC TGCCACCGTG CAGAGCTCTC TCCGCTCAGC TGCCCCCCCA 
GAAAAGTCTC TGAGTTACTC AGCCAGCTTT CCCGAGGCCC AGCCTGGAGT GCGAGAGGTC 
CCTGCAGCCA ACAGCAGCCC CTCATCTGCC AAGGCTACAG GGGGGACCTC AGAGTTCCCA 
GCCCCCAGCA GCAGGGACCA CAGGAAGCTT CAGTCTGGAG GAGACGGCCG AAGCCAAATG 
ATAAAGAGTG ACTCTCTGCC CTCCTTCCGC CTCTCCACCT CTGCTCTGGA GTCACATTTC 
CAGGATCCAC AGGTGCCCAT CGCATCAGGC CACCGAGGCA GGGCACTGTC AGTAACTGCT 
GCCACAGGAG AACCCAAAGG GAGAGAGCTC GCCCAGCCTC CCCCAGTCAG GAAACAGAAT 
GCGTGCAGAG AGGCGACCAG AGCACCCCCA GCCCCAAGCA CAGATCGCTC CCTCCCTCTT 
TCCTCAGAGA AAGACTTCGT GGTTCGGCAG AGAAGGGGCA AGGAGACCTT AAGGAGCAGT 
CCTCACAAAA AGGCCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242975.1
CDS	1238..8518
Translation	MDMSDPNFWTVLSNFTLPHLRSGNRLRRTQSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_011244673.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244673.3

ORF Insert Sequence:

ATGGATATGT CTGACCCCAA TTTCTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGCACACAA AGTTGCCGAA CAAGCAACCG GAAGAGTTTG 
ATAGGCAATG GCCAGTCTCC AGCACTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGACAG TCCAAGGAAT TTCTCCCCCA GTGCCTCAGC CCATTTCTCA 
TTTGCGAGGA GAACGGATGG GCGCCGCTGG TCTCTGGCTT CTCTCCCTTC CTCAGGCTAT 
GGGACAAATA CCCCAAGCTC CACCGTCTCC TCATCCTGTT CTTCCCAGGA GAAGTTGCAC 
CAGCTACCAT ACCAGCCAAC CCCAGATGAA TTACACTTCT TATCCAAACA CTTCTGCACA 
ACAGAAAGTA TCGCCACTGA GAACCGGTGC AGAAACACAC CCATGCGTCC ACGTTCCCGG 
AGTCTCAGCC CTGGACGGTC CCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCCACA GCTCAGATGG AAGAGCGTCT GAAGGAGATC 
ATCACCAGCT ACTCTCCAGA CCATGTTCTC CCCTTGGCAG ATGGGGTACT TAGTTTCACT 
CACCATCAGA TCATTGAGCT GGCTCGGGAC TGTTTGGATA AATCTCACCA GGGTCTCATC 
ACGTCGAGAT ACTTTTTTGA GTTGCAGCAC AAACTGGACA AGTTGCTCCA GGAGGCTCAC 
GATCGCTCTG AAAGTGGAGA ACTGGCATTT ATCAAGCAAC TAGTCCGAAA GATCCTAATT 
GTCATTGCCC GCCCCGCTCG GTTATTGGAG TGTTTGGAAT TTGATCCTGA AGAATTTTAT 
TACCTATTGG AAGCTGCAGA AGGCCATGCC AAAGAAGGCC AAGGAATCAA AACTGACATC 
CCTAGGTATA TCATCAGCCA GCTGGGACTC AATAAGGACC CTTTAGAAGA AATGGCTCAG 
TTGGGGAATT ACGACAGTAG GACAGCAGAG ACACCAGAGA TGGATGAGTC AGTGAGTAGC 
TCAAATACTT CCCTGAGACT TCGAAGGAAA CCCCGAGAGA GTGATTTTGA AACAATTAAA 
TTGATCAGCA ATGGAGCCTA CGGGGCAGTC TACTTTGTTC GGCACAAAGA GTCCCGCCAG 
AGGTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTCATCC TTCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGAGACAT CCTGACTTTC GCAGAGAACC CCTTTGTGGT CAGCATGTAT 
TGCTCCTTTG AAACGAGGCG TCACTTATGC ATGGTCATGG AGTATGTAGA AGGGGGAGAC 
TGTGCGACCC TAATGAAAAA CATGGGACCT CTCCCAGTTG ATATGGCCAG AATGTATTTC 
GCCGAGACCG TCTTGGCCTT GGAGTACCTG CATAATTACG GAATCGTACA CAGGGACTTG 
AAGCCAGACA ACCTGTTGGT CACCTCCATG GGGCACATAA AACTGACTGA CTTCGGCTTG 
TCTAAGGTGG GATTAATGAG CATGACCACC AATCTCTATG AAGGCCACAT AGAGAAGGAC 
GCTCGAGAGT TCTTAGATAA ACAGGTCTGT GGTACACCTG AGTACATTGC CCCCGAGGTG 
ATTCTGAGAC AGGGCTACGG GAAACCCGTG GACTGGTGGG CCATGGGCAT TATCCTCTAT 
GAATTTCTGG TCGGATGTGT GCCTTTCTTT GGGGACACTC CAGAAGAGCT ATTTGGACAA 
GTCATCAGTG ATGAAATCAA CTGGCCTGAA AAGGACGAGG CCCCTCCTCC AGACGCTCAG 
GAGCTGATTA CCTTGCTCCT CAGGCAGAAT CCGCTGGAGA GGCTGGGAAC AGGTGGAGCC 
TATGAAGTGA AGCAGCATCG TTTCTTCCGC TCCTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCGGAAT TTATTCCCCA ACTGGAATCG GAGGATGACA CAAGTTATTT TGATACTCGG 
TCAGAGAAGT ATCATCACAT GGAGACGGAG GAGGAGGACG ATACAAACGA TGAGGACTTC 
ACCGTGGAGA TAAGGCAGTT TTCTTCCTGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGCA TAACTCAAAA TTCAGGAGAA GACAAAGATG ACTCTGAGGA CAAGACCAAA 
AGCACAACGT TGCCATCCAC AGAGACACTC AGCTGGAGTT CCGAATACTC TGAAATGCAA 
CAGTTATCGA CCTCCAACTC TTCAGATACT GAAAGCAACA GGTGCAAACT CAGCTCTGGC 
TTGCTCCCCA AGCTGGCTAT TTCGACAGAT GGGGAACAAG ATGAGGCTGT CCCTTGCTCT 
GGAGACCCCA GAGAGGAGCC AGAGAAACCT GTCCCTCCCT CTGAGGAGTG TACTCAGGAG 
GAGCCCGAGG TCACCACCCC AGCCAGCACC ATCAGCAGTT CCACACTGTC AGTTGGCAGT 
TTTTCAGAGC ACTTGGATCA GATAAATGGG CGAAGCGAGT GTGTGGACAG TACAGATAAT 
TCCTCAAAGC CATCCAGTGA ACCCACTTCT CACGTGGCTC GACAGCGCTT AGAAAGCACA 
GAGAAAAAGA AAATTTCTGG GAAAGTCACA AAGTCCCTCT CGGCCAGTGC TCTGTCCCTC 
ATGATCCCAG GAGATATGTT CGCTGTATCT CCATTGGGAA GCCCAATGTC CCCACACTCC 
CTGTCTTCAG ACCCTTCTTC TTCACGGGAT TCCTCTCCCA GCCGAGACTC TTCTGCAGCA 
TCTGCCAGTC CGCATCAGCC CATTGTCATC CACAGCTCAG GCAAGAACTA TGGGTTCACC 
ATCCGTGCTA TCCGCGTGTA CGTGGGGGAC AGTGACATCT ACACAGTGCA CCATATCGTC 
TGGAACGTAG AAGAAGGAAG TCCCGCATAC CAGGCAGGAC TGAAGGCCGG GGATCTGATC 
ACACACATCA ACGGAGAGCC GGTGCACGGC CTCGTCCACA CGGAAGTTAT CGAGCTCCTG 
CTGAAGAGTG GGAATAAGGT GTCTATCACC ACTACTCCAT TTGAAAACAC ATCAATCAAA 
ACGGGACCAG CCAGGAGAAA CAGTTACAAG GGCCGGATGG TGAGACGAAG CAAGAAGTCC 
AAGAAGAAGG AGAGTCTAGA AAGGAGGAGA TCTCTCTTCA AGAAGCTGGC CAAGCAGCCT 
TCTCCTTTGC TCCACACCAG CCGAAGTTTC TCCTGCTTAA ACCGGTCCCT GTCATCTGGA 
GAGAGCCTCC CGGGTTCCCC AACTCACAGC TTGTCCCCGA GGTCTCCAAC ACCCAGTTAT 
CGTTCTACTC CCGATTTTCC GTCAGGTACA AATTCCTCCC AGAGCAGCTC CCCAAGTTCA 
AGTGCCCCCA ATTCTCCAGC AGGTTCAGGG CACATCCGGC CCAGCACCCT CCATGGCCTG 
GCTCCCAAAC TCAGCGGGCA GCGATACCGC TCTGGAAGAC GGAAGTCGGC TGGCAGCATC 
CCTCTCTCCC CGCTGGCCAG GACACCCTCT CCCACTCCAC AGCCTACCTC TCCTCAGCGT 
TCACCATCCC CACTGTTGGG ACACTCACTG GGCAATGCCA AGATCACTCA GGCCTTTCCT 
AGCAAGATGC ACTCTCCCCC CACAATCGTC AGACACATCG TGAGGCCCAA GAGTGCAGAG 
CCGCCCCGCT CCCCACTGCT GAAACGGGTG CAGTCGGAGG AAAAGTTGTC ACCCTCCTAT 
GGCAGTGACA AGAAGCTTCT GTGCTCCCGC AAGCATAGCC TAGAGGTGAC ACAAGAGGAG 
GTACAGAGGG AGCAGTGTCA GCGGGAAGTG ACACTGCAGA GCCTGGAAGA GAATGTGTGT 
GACGCTCCTT CCCTCAGTCG GGCCAGGCCA GTGGAGCAAG GCTGTCTGAA ACGCCCCGTG 
TCCCGGAAGG TGGGCAGGCA AGAGTCTGTG GATGACCTGG ACCGGGACAA GCTGAAAGCC 
AAGGTTGTCG TAAAGAAACC AGAAGAGAAA CATGAATCGC ACCAGAAACC TCACAGCCTT 
GGTGGTGATT CGGAAAGCTA TGCTCTCTTC AGGCTAGAGG AGAGAGAGAA AAAAGTGTAC 
TCCAAGGGGT TGGAAAGGTC AGGCCATTTT GAAAACACAT CAGCAGAGTT GCCTTCTGTG 
GGCAGCCTGC TGAAGGACAC TCTTCACAAG CAGGCCAGCG TGAGGGCCAG CGAGGGGGTG 
ACCTCAGACG GGGCAGCTTG CAGCCTGACA CCAGGGGAGC ACAGCCAGTC TCTAGGTGAC 
TTTAAGCGGG CCTCAGCTTC TGGCATTCTC CATGATAGTG TGTGCCCCAT CTCTGATAGG 
CCTGCTCCTG GAAAGGTTGA ATACTCGGAG AAGGCCTCTC AGGCCAAAGA GCTCCTTCGA 
AGTGAAAAAC TAGACAGCAA GCTGGCCAAT ATTGATTACC TCAGAAAGAA AATGTCATTG 
GATGACAAAG ATGACAGCCA CTGTGCCATC CTGAAACCCA AGATAACATC TAGCGCCCAT 
GAATGTCTGC CAGGGAACCC CATACGGCCC ATGGCAGGGC AACAAGAGAC CCCGCCAGCC 
TCTGAAAACC GGGCATTCAT CAACAGTACC CACACACCTC AGATGAGTGC AGTTTCCTTT 
GTTCCTCTCA AAGCCTTAGC TGGCCGGGTA GAGAACGGAG GGGAGAAAGC AGGCTTAGCT 
GCTCCCGAGT CCCCTGTCAG GAAGAGCCCC TCCGAGTATA AGCTAGAGGG CAGGTCAGTT 
TCATGTCTCA AGCCGATCGA GGGCACACTG GACATTGCTC TCCTGTCTGG ACCTCACGCC 
TCCAAAACAG AGTTGCTTTC CCCAGAGCCT GCACAGAGTC CCAGCCCAGG CATCAACGTG 
GGACCATGTG TGCCACTAGC TCTTCCTGGG AGCAGTGGGA AAAAGGGAGA CTCCACCAGC 
CTGAGAGAGC CTTCCTCAGC CAACTTAAAA GTAAATAAAT CTTATCTGCT GGAGCCTCGG 
TTCCTACCCC CGAGCCGGGC TCTCCAGGAC TCTCTCGCAG CCTCTGGGCC AGAACCGAAG 
TCAAAGCCGG AAAGGAAGCT CATTCATCCT TCTGCCAGGA GCCCAGCAAC TGTCACAGAG 
AGCAATCTTC AGCAGAAAGA GGGTGGTCCC GCCACACACC AAGACCGCTC CACTGACACC 
AGGAACCTCC CTGGCCCAGG GCAGACCCTA CACAATGTGG ACCTACCCAG GCTGTGTACA 
CGTGCCCCAC TCCCACCGGA AGGGACGCCC GCAAAGGAGA AGCCATGTCT GAAGGAACCC 
TCTGCCAAGG TGAAAAGCGA GTGGTCTGCC GTGAGGGATG ACGGACACAG AGATCCCTGT 
GCGAAGCTGT GCCCGGCAGA GACTGGTAAA GCCAGCGACA GTTCCAAACC CCTGCCTTCC 
GGGGGGAGGA CCCAACCCGA TTTCTACAAG CAGACCCAGA CTTCGGAGAA AGCATGGGCG 
CATGCAAAAA CAAACCACAA AGATAGCCAA GATGAGGTGA AGTCCCTGGC CAGGGAGGAC 
TCAGCTTCAC TTTTATATGA AAAGGAGATA GGCCGGGCAC GAAAAGGTCC TGAACCCAAA 
CCGGAAGTTC CTGCTACCCG GTGCCCTCCT CAGCCACCAG GAATTGAGGG TGAGAAGCGA 
GAAAAGCTCT CCGCTGCCCC CTCTTTGCAG AAACAGGCTC CCAAAGAGCC AGACAGGAAG 
GAACAGACTT CGCAAAGGCC TGGAGGTAGT GGCCCTCAAC AACCCCCACC CACCAAAGAG 
CTGTCTAACT CAGCATCCTG GCAGCACGGC AGTTCTCCGA GTCACACTTT AAAGAAGGAG 
CCCGGGACCA AAGCTGCCGC TGCAGAACCA AGCACCAGCC TTCATGACAC TCCCCGATCT 
GCTACAGCCA CCACCACTGC CATTGCCACC ACCACCACTA CCACCAGTGC CGGGCACAGT 
GACTGCAGTA GCCATAAGGC CCGGCCTGGC CCTGACCCCA GCCCTTCAAA GTCTAAGCAC 
CAAGACAGGT CCCTCTCCTC ACAGAAGCTG AGTGCTGGCT CTGCAAAAGG CAAAGAGCCT 
GTCACTCAAC CCCTGGGTGG TTCCATCAGA GAAGGCAAGG GTGGCAGCAA GGGTCCAGTG 
GACACATTTT CTGCTGTCCT GACCACCCAG GGCAAAGCAA GTGATGTGCT TGTGCAGGGA 
GAAGGTCGGG TCTCAATCAT TGTCCACACT GAAGAGTGTC CTCTCGATGC CAAACTGAAA 
AACACCAATG GAGGGTGTCC CCCAGAGATG CAGGCGAAGC ATCCACCCAG ACAAGGACAT 
CTCAGTGAAG CAGCAGACCA GAAGCCACTC ATTGCTGGTG AGAAGCAAAG CCCGTCTCCA 
AAGCATCCCA AACCATCCAC TGTGAAAGAT TACCCCAGTC TGTGCAGACA GACAGACAGA 
AGCCCAAGCC ATCAGGCTAC CACTGGGGAC AGGAAGGCAG AAGGAAAGAA ATGCACAGAC 
GCACTTTATG TCGCAGCCCC AGAGGGCTAC AAGCCAGAGG CCAGCCCTTC TCTCCACCAC 
GGCGAGACCG GACTCAGAGG CTCAGAGAGG CCACCCATGG GCATGGGGAA GGGCTTCTCT 
GAGCCCAAGG GGAAAGGGCC AGGTCCCCAG AAGTCACTGG CTGAAACAGG CAAGCCCAGC 
GGTATGAAAA GGTCACCCTC TGCCACCGTG CAGAGCTCTC TCCGCTCAGC TGCCCCCCCA 
GAAAAGTCTC TGAGTTACTC AGCCAGCTTT CCCGAGGCCC AGCCTGGAGT GCGAGAGGTC 
CCTGCAGCCA ACAGCAGCCC CTCATCTGCC AAGGCTACAG GGGGGACCTC AGAGTTCCCA 
GCCCCCAGCA GCAGGGACCA CAGGAAGCTT CAGTCTGGAG GAGACGGCCG AAGCCAAATG 
ATAAAGAGTG ACTCTCTGCC CTCCTTCCGC CTCTCCACCT CTGCTCTGGA GTCACATTTC 
CAGGATCCAC AGGTGCCCAT CGCATCAGGC CACCGAGGCA GGGCACTGTC AGTAACTGCT 
GCCACAGGAG AACCCAAAGG GAGAGAGCTC GCCCAGCCTC CCCCAGTCAG GAAACAGAAT 
GCGTGCAGAG AGGCGACCAG AGCACCCCCA GCCCCAAGCA CAGATCGCTC CCTCCCTCTT 
TCCTCAGAGA AAGACTTCGT GGTTCGGCAG AGAAGGGGCA AGGAGACCTT AAGGAGCAGT 
CCTCACAAAA AGGCCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_011244674.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242976.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X20, mRNA.

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CloneID	OMu74762
Clone ID Related Accession (Same CDS sequence)	XM_011244674.3 , XM_011244674.2
Accession Version	XM_011244674.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244674.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATGT CTGACCCCAA TTTCTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGCACACAA AGTTGCCGAA CAAGCAACCG GAAGAGTTTG 
ATAGGCAATG GCCAGTCTCC AGCACTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGACAG TCCAAGGAAT TTCTCCCCCA GTGCCTCAGC CCATTTCTCA 
TTTGCGAGGA GAACGGATGG GCGCCGCTGG TCTCTGGCTT CTCTCCCTTC CTCAGGCTAT 
GGGACAAATA CCCCAAGCTC CACCGTCTCC TCATCCTGTT CTTCCCAGGA GAAGTTGCAC 
CAGCTACCAT ACCAGCCAAC CCCAGATGAA TTACACTTCT TATCCAAACA CTTCTGCACA 
ACAGAAAGTA TCGCCACTGA GAACCGGTGC AGAAACACAC CCATGCGTCC ACGTTCCCGG 
AGTCTCAGCC CTGGACGGTC CCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCCACA GCTCAGATGG AAGAGCGTCT GAAGGAGATC 
ATCACCAGCT ACTCTCCAGA CCATGTTCTC CCCTTGGCAG ATGGGGTACT TAGTTTCACT 
CACCATCAGA TCATTGAGCT GGCTCGGGAC TGTTTGGATA AATCTCACCA GGGTCTCATC 
ACGTCGAGAT ACTTTTTTGA GTTGCAGCAC AAACTGGACA AGTTGCTCCA GGAGGCTCAC 
GATCGCTCTG AAAGTGGAGA ACTGGCATTT ATCAAGCAAC TAGTCCGAAA GATCCTAATT 
GTCATTGCCC GCCCCGCTCG GTTATTGGAG TGTTTGGAAT TTGATCCTGA AGAATTTTAT 
TACCTATTGG AAGCTGCAGA AGGCCATGCC AAAGAAGGCC AAGGAATCAA AACTGACATC 
CCTAGGTATA TCATCAGCCA GCTGGGACTC AATAAGGACC CTTTAGAAGA AATGGCTCAG 
TTGGGGAATT ACGACAGTAG GACAGCAGAG ACACCAGAGA TGGATGAGTC AGTGAGTAGC 
TCAAATACTT CCCTGAGACT TCGAAGGAAA CCCCGAGAGA GTGATTTTGA AACAATTAAA 
TTGATCAGCA ATGGAGCCTA CGGGGCAGTC TACTTTGTTC GGCACAAAGA GTCCCGCCAG 
AGGTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTCATCC TTCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGAGACAT CCTGACTTTC GCAGAGAACC CCTTTGTGGT CAGCATGTAT 
TGCTCCTTTG AAACGAGGCG TCACTTATGC ATGGTCATGG AGTATGTAGA AGGGGGAGAC 
TGTGCGACCC TAATGAAAAA CATGGGACCT CTCCCAGTTG ATATGGCCAG AATGTATTTC 
GCCGAGACCG TCTTGGCCTT GGAGTACCTG CATAATTACG GAATCGTACA CAGGGACTTG 
AAGCCAGACA ACCTGTTGGT CACCTCCATG GGGCACATAA AACTGACTGA CTTCGGCTTG 
TCTAAGGTGG GATTAATGAG CATGACCACC AATCTCTATG AAGGCCACAT AGAGAAGGAC 
GCTCGAGAGT TCTTAGATAA ACAGGTCTGT GGTACACCTG AGTACATTGC CCCCGAGGTG 
ATTCTGAGAC AGGGCTACGG GAAACCCGTG GACTGGTGGG CCATGGGCAT TATCCTCTAT 
GAATTTCTGG TCGGATGTGT GCCTTTCTTT GGGGACACTC CAGAAGAGCT ATTTGGACAA 
GTCATCAGTG ATGAAATCAA CTGGCCTGAA AAGGACGAGG CCCCTCCTCC AGACGCTCAG 
GAGCTGATTA CCTTGCTCCT CAGGCAGAAT CCGCTGGAGA GGCTGGGAAC AGGTGGAGCC 
TATGAAGTGA AGCAGCATCG TTTCTTCCGC TCCTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCGGAAT TTATTCCCCA ACTGGAATCG GAGGATGACA CAAGTTATTT TGATACTCGG 
TCAGAGAAGT ATCATCACAT GGAGACGGAG GAGGAGGACG ATACAAACGA TGAGGACTTC 
ACCGTGGAGA TAAGGCAGTT TTCTTCCTGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGCA TAACTCAAAA TTCAGGAGAA GACAAAGATG ACTCTGAGGA CAAGACCAAA 
AGCACAACGT TGCCATCCAC AGAGACACTC AGCTGGAGTT CCGAATACTC TGAAATGCAA 
CAGTTATCGA CCTCCAACTC TTCAGATACT GAAAGCAACA GGTGCAAACT CAGCTCTGGC 
TTGCTCCCCA AGCTGGCTAT TTCGACAGAT GGGGAACAAG ATGAGGCTGT CCCTTGCTCT 
GGAGACCCCA GAGAGGAGCC AGAGAAACCT GTCCCTCCCT CTGAGGAGTG TACTCAGGAG 
GAGCCCGAGG TCACCACCCC AGCCAGCACC ATCAGCAGTT CCACACTGTC AGATATGTTC 
GCTGTATCTC CATTGGGAAG CCCAATGTCC CCACACTCCC TGTCTTCAGA CCCTTCTTCT 
TCACGGGATT CCTCTCCCAG CCGAGACTCT TCTGCAGCAT CTGCCAGTCC GCATCAGCCC 
ATTGTCATCC ACAGCTCAGG CAAGAACTAT GGGTTCACCA TCCGTGCTAT CCGCGTGTAC 
GTGGGGGACA GTGACATCTA CACAGTGCAC CATATCGTCT GGAACGTAGA AGAAGGAAGT 
CCCGCATACC AGGCAGGACT GAAGGCCGGG GATCTGATCA CACACATCAA CGGAGAGCCG 
GTGCACGGCC TCGTCCACAC GGAAGTTATC GAGCTCCTGC TGAAGAGTGG GAATAAGGTG 
TCTATCACCA CTACTCCATT TGAAAACACA TCAATCAAAA CGGGACCAGC CAGGAGAAAC 
AGTTACAAGG GCCGGATGGT GAGACGAAGC AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA 
AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC AAGCAGCCTT CTCCTTTGCT CCACACCAGC 
CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG TCATCTGGAG AGAGCCTCCC GGGTTCCCCA 
ACTCACAGCT TGTCCCCGAG GTCTCCAACA CCCAGTTATC GTTCTACTCC CGATTTTCCG 
TCAGGTACAA ATTCCTCCCA GAGCAGCTCC CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA 
GGTTCAGGGC ACATCCGGCC CAGCACCCTC CATGGCCTGG CTCCCAAACT CAGCGGGCAG 
CGATACCGCT CTGGAAGACG GAAGTCGGCT GGCAGCATCC CTCTCTCCCC GCTGGCCAGG 
ACACCCTCTC CCACTCCACA GCCTACCTCT CCTCAGCGTT CACCATCCCC ACTGTTGGGA 
CACTCACTGG GCAATGCCAA GATCACTCAG GCCTTTCCTA GCAAGATGCA CTCTCCCCCC 
ACAATCGTCA GACACATCGT GAGGCCCAAG AGTGCAGAGC CGCCCCGCTC CCCACTGCTG 
AAACGGGTGC AGTCGGAGGA AAAGTTGTCA CCCTCCTATG GCAGTGACAA GAAGCTTCTG 
TGCTCCCGCA AGCATAGCCT AGAGGTGACA CAAGAGGAGG TACAGAGGGA GCAGTGTCAG 
CGGGAAGTGA CACTGCAGAG CCTGGAAGAG AATGTGTGTG ACGCTCCTTC CCTCAGTCGG 
GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA 
GAGTCTGTGG ATGACCTGGA CCGGGACAAG CTGAAAGCCA AGGTTGTCGT AAAGAAACCA 
GAAGAGAAAC ATGAATCGCA CCAGAAACCT CACAGCCTTG GTGGTGATTC GGAAAGCTAT 
GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA AAAGTGTACT CCAAGGGGTT GGAAAGGTCA 
GGCCATTTTG AAAACACATC AGCAGAGTTG CCTTCTGTGG GCAGCCTGCT GAAGGACACT 
CTTCACAAGC AGGCCAGCGT GAGGGCCAGC GAGGGGGTGA CCTCAGACGG GGCAGCTTGC 
AGCCTGACAC CAGGGGAGCA CAGCCAGTCT CTAGGTGACT TTAAGCGGGC CTCAGCTTCT 
GGCATTCTCC ATGATAGTGT GTGCCCCATC TCTGATAGGC CTGCTCCTGG AAAGGTTGAA 
TACTCGGAGA AGGCCTCTCA GGCCAAAGAG CTCCTTCGAA GTGAAAAACT AGACAGCAAG 
CTGGCCAATA TTGATTACCT CAGAAAGAAA ATGTCATTGG ATGACAAAGA TGACAGCCAC 
TGTGCCATCC TGAAACCCAA GATAACATCT AGCGCCCATG AATGTCTGCC AGGGAACCCC 
ATACGGCCCA TGGCAGGGCA ACAAGAGACC CCGCCAGCCT CTGAAAACCG GGCATTCATC 
AACAGTACCC ACACACCTCA GATGAGTGCA GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT 
GGCCGGGTAG AGAACGGAGG GGAGAAAGCA GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG 
AAGAGCCCCT CCGAGTATAA GCTAGAGGGC AGGTCAGTTT CATGTCTCAA GCCGATCGAG 
GGCACACTGG ACATTGCTCT CCTGTCTGGA CCTCACGCCT CCAAAACAGA GTTGCTTTCC 
CCAGAGCCTG CACAGAGTCC CAGCCCAGGC ATCAACGTGG GACCATGTGT GCCACTAGCT 
CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC TCCACCAGCC TGAGAGAGCC TTCCTCAGCC 
AACTTAAAAG TAAATAAATC TTATCTGCTG GAGCCTCGGT TCCTACCCCC GAGCCGGGCT 
CTCCAGGACT CTCTCGCAGC CTCTGGGCCA GAACCGAAGT CAAAGCCGGA AAGGAAGCTC 
ATTCATCCTT CTGCCAGGAG CCCAGCAACT GTCACAGAGA GCAATCTTCA GCAGAAAGAG 
GGTGGTCCCG CCACACACCA AGACCGCTCC ACTGACACCA GGAACCTCCC TGGCCCAGGG 
CAGACCCTAC ACAATGTGGA CCTACCCAGG CTGTGTACAC GTGCCCCACT CCCACCGGAA 
GGGACGCCCG CAAAGGAGAA GCCATGTCTG AAGGAACCCT CTGCCAAGGT GAAAAGCGAG 
TGGTCTGCCG TGAGGGATGA CGGACACAGA GATCCCTGTG CGAAGCTGTG CCCGGCAGAG 
ACTGGTAAAG CCAGCGACAG TTCCAAACCC CTGCCTTCCG GGGGGAGGAC CCAACCCGAT 
TTCTACAAGC AGACCCAGAC TTCGGAGAAA GCATGGGCGC ATGCAAAAAC AAACCACAAA 
GATAGCCAAG ATGAGGTGAA GTCCCTGGCC AGGGAGGACT CAGCTTCACT TTTATATGAA 
AAGGAGATAG GCCGGGCACG AAAAGGTCCT GAACCCAAAC CGGAAGTTCC TGCTACCCGG 
TGCCCTCCTC AGCCACCAGG AATTGAGGGT GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC 
TCTTTGCAGA AACAGGCTCC CAAAGAGCCA GACAGGAAGG AACAGACTTC GCAAAGGCCT 
GGAGGTAGTG GCCCTCAACA ACCCCCACCC ACCAAAGAGC TGTCTAACTC AGCATCCTGG 
CAGCACGGCA GTTCTCCGAG TCACACTTTA AAGAAGGAGC CCGGGACCAA AGCTGCCGCT 
GCAGAACCAA GCACCAGCCT TCATGACACT CCCCGATCTG CTACAGCCAC CACCACTGCC 
ATTGCCACCA CCACCACTAC CACCAGTGCC GGGCACAGTG ACTGCAGTAG CCATAAGGCC 
CGGCCTGGCC CTGACCCCAG CCCTTCAAAG TCTAAGCACC AAGACAGGTC CCTCTCCTCA 
CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC AAAGAGCCTG TCACTCAACC CCTGGGTGGT 
TCCATCAGAG AAGGCAAGGG TGGCAGCAAG GGTCCAGTGG ACACATTTTC TGCTGTCCTG 
ACCACCCAGG GCAAAGCAAG TGATGTGCTT GTGCAGGGAG AAGGTCGGGT CTCAATCATT 
GTCCACACTG AAGAGTGTCC TCTCGATGCC AAACTGAAAA ACACCAATGG AGGGTGTCCC 
CCAGAGATGC AGGCGAAGCA TCCACCCAGA CAAGGACATC TCAGTGAAGC AGCAGACCAG 
AAGCCACTCA TTGCTGGTGA GAAGCAAAGC CCGTCTCCAA AGCATCCCAA ACCATCCACT 
GTGAAAGATT ACCCCAGTCT GTGCAGACAG ACAGACAGAA GCCCAAGCCA TCAGGCTACC 
ACTGGGGACA GGAAGGCAGA AGGAAAGAAA TGCACAGACG CACTTTATGT CGCAGCCCCA 
GAGGGCTACA AGCCAGAGGC CAGCCCTTCT CTCCACCACG GCGAGACCGG ACTCAGAGGC 
TCAGAGAGGC CACCCATGGG CATGGGGAAG GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA 
GGTCCCCAGA AGTCACTGGC TGAAACAGGC AAGCCCAGCG GTATGAAAAG GTCACCCTCT 
GCCACCGTGC AGAGCTCTCT CCGCTCAGCT GCCCCCCCAG AAAAGTCTCT GAGTTACTCA 
GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC 
TCATCTGCCA AGGCTACAGG GGGGACCTCA GAGTTCCCAG CCCCCAGCAG CAGGGACCAC 
AGGAAGCTTC AGTCTGGAGG AGACGGCCGA AGCCAAATGA TAAAGAGTGA CTCTCTGCCC 
TCCTTCCGCC TCTCCACCTC TGCTCTGGAG TCACATTTCC AGGATCCACA GGTGCCCATC 
GCATCAGGCC ACCGAGGCAG GGCACTGTCA GTAACTGCTG CCACAGGAGA ACCCAAAGGG 
AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG AAACAGAATG CGTGCAGAGA GGCGACCAGA 
GCACCCCCAG CCCCAAGCAC AGATCGCTCC CTCCCTCTTT CCTCAGAGAA AGACTTCGTG 
GTTCGGCAGA GAAGGGGCAA GGAGACCTTA AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242976.1
CDS	690..7769
Translation	MDMSDPNFWTVLSNFTLPHLRSGNRLRRTQSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_011244674.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244674.3

ORF Insert Sequence:

ATGGATATGT CTGACCCCAA TTTCTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGCACACAA AGTTGCCGAA CAAGCAACCG GAAGAGTTTG 
ATAGGCAATG GCCAGTCTCC AGCACTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGACAG TCCAAGGAAT TTCTCCCCCA GTGCCTCAGC CCATTTCTCA 
TTTGCGAGGA GAACGGATGG GCGCCGCTGG TCTCTGGCTT CTCTCCCTTC CTCAGGCTAT 
GGGACAAATA CCCCAAGCTC CACCGTCTCC TCATCCTGTT CTTCCCAGGA GAAGTTGCAC 
CAGCTACCAT ACCAGCCAAC CCCAGATGAA TTACACTTCT TATCCAAACA CTTCTGCACA 
ACAGAAAGTA TCGCCACTGA GAACCGGTGC AGAAACACAC CCATGCGTCC ACGTTCCCGG 
AGTCTCAGCC CTGGACGGTC CCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCCACA GCTCAGATGG AAGAGCGTCT GAAGGAGATC 
ATCACCAGCT ACTCTCCAGA CCATGTTCTC CCCTTGGCAG ATGGGGTACT TAGTTTCACT 
CACCATCAGA TCATTGAGCT GGCTCGGGAC TGTTTGGATA AATCTCACCA GGGTCTCATC 
ACGTCGAGAT ACTTTTTTGA GTTGCAGCAC AAACTGGACA AGTTGCTCCA GGAGGCTCAC 
GATCGCTCTG AAAGTGGAGA ACTGGCATTT ATCAAGCAAC TAGTCCGAAA GATCCTAATT 
GTCATTGCCC GCCCCGCTCG GTTATTGGAG TGTTTGGAAT TTGATCCTGA AGAATTTTAT 
TACCTATTGG AAGCTGCAGA AGGCCATGCC AAAGAAGGCC AAGGAATCAA AACTGACATC 
CCTAGGTATA TCATCAGCCA GCTGGGACTC AATAAGGACC CTTTAGAAGA AATGGCTCAG 
TTGGGGAATT ACGACAGTAG GACAGCAGAG ACACCAGAGA TGGATGAGTC AGTGAGTAGC 
TCAAATACTT CCCTGAGACT TCGAAGGAAA CCCCGAGAGA GTGATTTTGA AACAATTAAA 
TTGATCAGCA ATGGAGCCTA CGGGGCAGTC TACTTTGTTC GGCACAAAGA GTCCCGCCAG 
AGGTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTCATCC TTCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGAGACAT CCTGACTTTC GCAGAGAACC CCTTTGTGGT CAGCATGTAT 
TGCTCCTTTG AAACGAGGCG TCACTTATGC ATGGTCATGG AGTATGTAGA AGGGGGAGAC 
TGTGCGACCC TAATGAAAAA CATGGGACCT CTCCCAGTTG ATATGGCCAG AATGTATTTC 
GCCGAGACCG TCTTGGCCTT GGAGTACCTG CATAATTACG GAATCGTACA CAGGGACTTG 
AAGCCAGACA ACCTGTTGGT CACCTCCATG GGGCACATAA AACTGACTGA CTTCGGCTTG 
TCTAAGGTGG GATTAATGAG CATGACCACC AATCTCTATG AAGGCCACAT AGAGAAGGAC 
GCTCGAGAGT TCTTAGATAA ACAGGTCTGT GGTACACCTG AGTACATTGC CCCCGAGGTG 
ATTCTGAGAC AGGGCTACGG GAAACCCGTG GACTGGTGGG CCATGGGCAT TATCCTCTAT 
GAATTTCTGG TCGGATGTGT GCCTTTCTTT GGGGACACTC CAGAAGAGCT ATTTGGACAA 
GTCATCAGTG ATGAAATCAA CTGGCCTGAA AAGGACGAGG CCCCTCCTCC AGACGCTCAG 
GAGCTGATTA CCTTGCTCCT CAGGCAGAAT CCGCTGGAGA GGCTGGGAAC AGGTGGAGCC 
TATGAAGTGA AGCAGCATCG TTTCTTCCGC TCCTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCGGAAT TTATTCCCCA ACTGGAATCG GAGGATGACA CAAGTTATTT TGATACTCGG 
TCAGAGAAGT ATCATCACAT GGAGACGGAG GAGGAGGACG ATACAAACGA TGAGGACTTC 
ACCGTGGAGA TAAGGCAGTT TTCTTCCTGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGCA TAACTCAAAA TTCAGGAGAA GACAAAGATG ACTCTGAGGA CAAGACCAAA 
AGCACAACGT TGCCATCCAC AGAGACACTC AGCTGGAGTT CCGAATACTC TGAAATGCAA 
CAGTTATCGA CCTCCAACTC TTCAGATACT GAAAGCAACA GGTGCAAACT CAGCTCTGGC 
TTGCTCCCCA AGCTGGCTAT TTCGACAGAT GGGGAACAAG ATGAGGCTGT CCCTTGCTCT 
GGAGACCCCA GAGAGGAGCC AGAGAAACCT GTCCCTCCCT CTGAGGAGTG TACTCAGGAG 
GAGCCCGAGG TCACCACCCC AGCCAGCACC ATCAGCAGTT CCACACTGTC AGATATGTTC 
GCTGTATCTC CATTGGGAAG CCCAATGTCC CCACACTCCC TGTCTTCAGA CCCTTCTTCT 
TCACGGGATT CCTCTCCCAG CCGAGACTCT TCTGCAGCAT CTGCCAGTCC GCATCAGCCC 
ATTGTCATCC ACAGCTCAGG CAAGAACTAT GGGTTCACCA TCCGTGCTAT CCGCGTGTAC 
GTGGGGGACA GTGACATCTA CACAGTGCAC CATATCGTCT GGAACGTAGA AGAAGGAAGT 
CCCGCATACC AGGCAGGACT GAAGGCCGGG GATCTGATCA CACACATCAA CGGAGAGCCG 
GTGCACGGCC TCGTCCACAC GGAAGTTATC GAGCTCCTGC TGAAGAGTGG GAATAAGGTG 
TCTATCACCA CTACTCCATT TGAAAACACA TCAATCAAAA CGGGACCAGC CAGGAGAAAC 
AGTTACAAGG GCCGGATGGT GAGACGAAGC AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA 
AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC AAGCAGCCTT CTCCTTTGCT CCACACCAGC 
CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG TCATCTGGAG AGAGCCTCCC GGGTTCCCCA 
ACTCACAGCT TGTCCCCGAG GTCTCCAACA CCCAGTTATC GTTCTACTCC CGATTTTCCG 
TCAGGTACAA ATTCCTCCCA GAGCAGCTCC CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA 
GGTTCAGGGC ACATCCGGCC CAGCACCCTC CATGGCCTGG CTCCCAAACT CAGCGGGCAG 
CGATACCGCT CTGGAAGACG GAAGTCGGCT GGCAGCATCC CTCTCTCCCC GCTGGCCAGG 
ACACCCTCTC CCACTCCACA GCCTACCTCT CCTCAGCGTT CACCATCCCC ACTGTTGGGA 
CACTCACTGG GCAATGCCAA GATCACTCAG GCCTTTCCTA GCAAGATGCA CTCTCCCCCC 
ACAATCGTCA GACACATCGT GAGGCCCAAG AGTGCAGAGC CGCCCCGCTC CCCACTGCTG 
AAACGGGTGC AGTCGGAGGA AAAGTTGTCA CCCTCCTATG GCAGTGACAA GAAGCTTCTG 
TGCTCCCGCA AGCATAGCCT AGAGGTGACA CAAGAGGAGG TACAGAGGGA GCAGTGTCAG 
CGGGAAGTGA CACTGCAGAG CCTGGAAGAG AATGTGTGTG ACGCTCCTTC CCTCAGTCGG 
GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA 
GAGTCTGTGG ATGACCTGGA CCGGGACAAG CTGAAAGCCA AGGTTGTCGT AAAGAAACCA 
GAAGAGAAAC ATGAATCGCA CCAGAAACCT CACAGCCTTG GTGGTGATTC GGAAAGCTAT 
GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA AAAGTGTACT CCAAGGGGTT GGAAAGGTCA 
GGCCATTTTG AAAACACATC AGCAGAGTTG CCTTCTGTGG GCAGCCTGCT GAAGGACACT 
CTTCACAAGC AGGCCAGCGT GAGGGCCAGC GAGGGGGTGA CCTCAGACGG GGCAGCTTGC 
AGCCTGACAC CAGGGGAGCA CAGCCAGTCT CTAGGTGACT TTAAGCGGGC CTCAGCTTCT 
GGCATTCTCC ATGATAGTGT GTGCCCCATC TCTGATAGGC CTGCTCCTGG AAAGGTTGAA 
TACTCGGAGA AGGCCTCTCA GGCCAAAGAG CTCCTTCGAA GTGAAAAACT AGACAGCAAG 
CTGGCCAATA TTGATTACCT CAGAAAGAAA ATGTCATTGG ATGACAAAGA TGACAGCCAC 
TGTGCCATCC TGAAACCCAA GATAACATCT AGCGCCCATG AATGTCTGCC AGGGAACCCC 
ATACGGCCCA TGGCAGGGCA ACAAGAGACC CCGCCAGCCT CTGAAAACCG GGCATTCATC 
AACAGTACCC ACACACCTCA GATGAGTGCA GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT 
GGCCGGGTAG AGAACGGAGG GGAGAAAGCA GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG 
AAGAGCCCCT CCGAGTATAA GCTAGAGGGC AGGTCAGTTT CATGTCTCAA GCCGATCGAG 
GGCACACTGG ACATTGCTCT CCTGTCTGGA CCTCACGCCT CCAAAACAGA GTTGCTTTCC 
CCAGAGCCTG CACAGAGTCC CAGCCCAGGC ATCAACGTGG GACCATGTGT GCCACTAGCT 
CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC TCCACCAGCC TGAGAGAGCC TTCCTCAGCC 
AACTTAAAAG TAAATAAATC TTATCTGCTG GAGCCTCGGT TCCTACCCCC GAGCCGGGCT 
CTCCAGGACT CTCTCGCAGC CTCTGGGCCA GAACCGAAGT CAAAGCCGGA AAGGAAGCTC 
ATTCATCCTT CTGCCAGGAG CCCAGCAACT GTCACAGAGA GCAATCTTCA GCAGAAAGAG 
GGTGGTCCCG CCACACACCA AGACCGCTCC ACTGACACCA GGAACCTCCC TGGCCCAGGG 
CAGACCCTAC ACAATGTGGA CCTACCCAGG CTGTGTACAC GTGCCCCACT CCCACCGGAA 
GGGACGCCCG CAAAGGAGAA GCCATGTCTG AAGGAACCCT CTGCCAAGGT GAAAAGCGAG 
TGGTCTGCCG TGAGGGATGA CGGACACAGA GATCCCTGTG CGAAGCTGTG CCCGGCAGAG 
ACTGGTAAAG CCAGCGACAG TTCCAAACCC CTGCCTTCCG GGGGGAGGAC CCAACCCGAT 
TTCTACAAGC AGACCCAGAC TTCGGAGAAA GCATGGGCGC ATGCAAAAAC AAACCACAAA 
GATAGCCAAG ATGAGGTGAA GTCCCTGGCC AGGGAGGACT CAGCTTCACT TTTATATGAA 
AAGGAGATAG GCCGGGCACG AAAAGGTCCT GAACCCAAAC CGGAAGTTCC TGCTACCCGG 
TGCCCTCCTC AGCCACCAGG AATTGAGGGT GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC 
TCTTTGCAGA AACAGGCTCC CAAAGAGCCA GACAGGAAGG AACAGACTTC GCAAAGGCCT 
GGAGGTAGTG GCCCTCAACA ACCCCCACCC ACCAAAGAGC TGTCTAACTC AGCATCCTGG 
CAGCACGGCA GTTCTCCGAG TCACACTTTA AAGAAGGAGC CCGGGACCAA AGCTGCCGCT 
GCAGAACCAA GCACCAGCCT TCATGACACT CCCCGATCTG CTACAGCCAC CACCACTGCC 
ATTGCCACCA CCACCACTAC CACCAGTGCC GGGCACAGTG ACTGCAGTAG CCATAAGGCC 
CGGCCTGGCC CTGACCCCAG CCCTTCAAAG TCTAAGCACC AAGACAGGTC CCTCTCCTCA 
CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC AAAGAGCCTG TCACTCAACC CCTGGGTGGT 
TCCATCAGAG AAGGCAAGGG TGGCAGCAAG GGTCCAGTGG ACACATTTTC TGCTGTCCTG 
ACCACCCAGG GCAAAGCAAG TGATGTGCTT GTGCAGGGAG AAGGTCGGGT CTCAATCATT 
GTCCACACTG AAGAGTGTCC TCTCGATGCC AAACTGAAAA ACACCAATGG AGGGTGTCCC 
CCAGAGATGC AGGCGAAGCA TCCACCCAGA CAAGGACATC TCAGTGAAGC AGCAGACCAG 
AAGCCACTCA TTGCTGGTGA GAAGCAAAGC CCGTCTCCAA AGCATCCCAA ACCATCCACT 
GTGAAAGATT ACCCCAGTCT GTGCAGACAG ACAGACAGAA GCCCAAGCCA TCAGGCTACC 
ACTGGGGACA GGAAGGCAGA AGGAAAGAAA TGCACAGACG CACTTTATGT CGCAGCCCCA 
GAGGGCTACA AGCCAGAGGC CAGCCCTTCT CTCCACCACG GCGAGACCGG ACTCAGAGGC 
TCAGAGAGGC CACCCATGGG CATGGGGAAG GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA 
GGTCCCCAGA AGTCACTGGC TGAAACAGGC AAGCCCAGCG GTATGAAAAG GTCACCCTCT 
GCCACCGTGC AGAGCTCTCT CCGCTCAGCT GCCCCCCCAG AAAAGTCTCT GAGTTACTCA 
GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC 
TCATCTGCCA AGGCTACAGG GGGGACCTCA GAGTTCCCAG CCCCCAGCAG CAGGGACCAC 
AGGAAGCTTC AGTCTGGAGG AGACGGCCGA AGCCAAATGA TAAAGAGTGA CTCTCTGCCC 
TCCTTCCGCC TCTCCACCTC TGCTCTGGAG TCACATTTCC AGGATCCACA GGTGCCCATC 
GCATCAGGCC ACCGAGGCAG GGCACTGTCA GTAACTGCTG CCACAGGAGA ACCCAAAGGG 
AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG AAACAGAATG CGTGCAGAGA GGCGACCAGA 
GCACCCCCAG CCCCAAGCAC AGATCGCTCC CTCCCTCTTT CCTCAGAGAA AGACTTCGTG 
GTTCGGCAGA GAAGGGGCAA GGAGACCTTA AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_030247307.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103167.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X8, mRNA.

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CloneID	OMu86254
Clone ID Related Accession (Same CDS sequence)	XM_030247307.1
Accession Version	XM_030247307.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGG AAGGGCAACC TCCGAAGAGC 
CATTTCAACT CAGCTCGACA TCGGCAGAGA CTAGTGGACC CAGCTGCTTC AAAAAAGGAG 
CTGAGCCTCC CCCGAAGAGG AAGTCTTTGC CGAACAAGCA ACCGGAAGAG TTTGATAGGC 
AATGGCCAGT CTCCAGCACT GCCTCGACCA CACTCACCTC TCTCTGCTCA TGCAGGAAAT 
AGCCCTCAAG ACAGTCCAAG GAATTTCTCC CCCAGTGCCT CAGCCCATTT CTCATTTGCG 
AGGAGAACGG ATGGGCGCCG CTGGTCTCTG GCTTCTCTCC CTTCCTCAGG CTATGGGACA 
AATACCCCAA GCTCCACCGT CTCCTCATCC TGTTCTTCCC AGGAGAAGTT GCACCAGCTA 
CCATACCAGC CAACCCCAGA TGAATTACAC TTCTTATCCA AACACTTCTG CACAACAGAA 
AGTATCGCCA CTGAGAACCG GTGCAGAAAC ACACCCATGC GTCCACGTTC CCGGAGTCTC 
AGCCCTGGAC GGTCCCCCGC CTGCTGTGAC CATGAAATAA TTATGATGAA CCATGTCTAC 
AAAGAAAGGT TCCCAAAGGC CACAGCTCAG ATGGAAGAGC GTCTGAAGGA GATCATCACC 
AGCTACTCTC CAGACCATGT TCTCCCCTTG GCAGATGGGG TACTTAGTTT CACTCACCAT 
CAGATCATTG AGCTGGCTCG GGACTGTTTG GATAAATCTC ACCAGGGTCT CATCACGTCG 
AGATACTTTT TTGAGTTGCA GCACAAACTG GACAAGTTGC TCCAGGAGGC TCACGATCGC 
TCTGAAAGTG GAGAACTGGC ATTTATCAAG CAACTAGTCC GAAAGATCCT AATTGTCATT 
GCCCGCCCCG CTCGGTTATT GGAGTGTTTG GAATTTGATC CTGAAGAATT TTATTACCTA 
TTGGAAGCTG CAGAAGGCCA TGCCAAAGAA GGCCAAGGAA TCAAAACTGA CATCCCTAGG 
TATATCATCA GCCAGCTGGG ACTCAATAAG GACCCTTTAG AAGAAATGGC TCAGTTGGGG 
AATTACGACA GTAGGACAGC AGAGACACCA GAGATGGATG AGTCAGTGAG TAGCTCAAAT 
ACTTCCCTGA GACTTCGAAG GAAACCCCGA GAGAGTGATT TTGAAACAAT TAAATTGATC 
AGCAATGGAG CCTACGGGGC AGTCTACTTT GTTCGGCACA AAGAGTCCCG CCAGAGGTTT 
GCCATGAAGA AGATCAACAA GCAGAACCTC ATCCTTCGGA ACCAGATCCA GCAGGCCTTC 
GTGGAGCGAG ACATCCTGAC TTTCGCAGAG AACCCCTTTG TGGTCAGCAT GTATTGCTCC 
TTTGAAACGA GGCGTCACTT ATGCATGGTC ATGGAGTATG TAGAAGGGGG AGACTGTGCG 
ACCCTAATGA AAAACATGGG ACCTCTCCCA GTTGATATGG CCAGAATGTA TTTCGCCGAG 
ACCGTCTTGG CCTTGGAGTA CCTGCATAAT TACGGAATCG TACACAGGGA CTTGAAGCCA 
GACAACCTGT TGGTCACCTC CATGGGGCAC ATAAAACTGA CTGACTTCGG CTTGTCTAAG 
GTGGGATTAA TGAGCATGAC CACCAATCTC TATGAAGGCC ACATAGAGAA GGACGCTCGA 
GAGTTCTTAG ATAAACAGGT CTGTGGTACA CCTGAGTACA TTGCCCCCGA GGTGATTCTG 
AGACAGGGCT ACGGGAAACC CGTGGACTGG TGGGCCATGG GCATTATCCT CTATGAATTT 
CTGGTCGGAT GTGTGCCTTT CTTTGGGGAC ACTCCAGAAG AGCTATTTGG ACAAGTCATC 
AGTGATGAAA TCAACTGGCC TGAAAAGGAC GAGGCCCCTC CTCCAGACGC TCAGGAGCTG 
ATTACCTTGC TCCTCAGGCA GAATCCGCTG GAGAGGCTGG GAACAGGTGG AGCCTATGAA 
GTGAAGCAGC ATCGTTTCTT CCGCTCCTTA GACTGGAACA GTTTGCTGAG ACAGAAGGCG 
GAATTTATTC CCCAACTGGA ATCGGAGGAT GACACAAGTT ATTTTGATAC TCGGTCAGAG 
AAGTATCATC ACATGGAGAC GGAGGAGGAG GACGATACAA ACGATGAGGA CTTCACCGTG 
GAGATAAGGC AGTTTTCTTC CTGTTCACAC AGGTTTTCAA AAGTTTTCAG CAGTATAGAT 
CGCATAACTC AAAATTCAGG AGAAGACAAA GATGACTCTG AGGACAAGAC CAAAAGCACA 
ACGTTGCCAT CCACAGAGAC ACTCAGCTGG AGTTCCGAAT ACTCTGAAAT GCAACAGTTA 
TCGACCTCCA ACTCTTCAGA TACTGAAAGC AACAGGTGCA AACTCAGCTC TGGCTTGCTC 
CCCAAGCTGG CTATTTCGAC AGATGGGGAA CAAGATGAGG CTGTCCCTTG CTCTGGAGAC 
CCCAGAGAGG AGCCAGAGAA ACCTGTCCCT CCCTCTGAGG AGTGTACTCA GGAGGAGCCC 
GAGGTCACCA CCCCAGCCAG CACCATCAGC AGTTCCACAC TGTCAGTTGG CAGTTTTTCA 
GAGCACTTGG ATCAGATAAA TGGGCGAAGC GAGTGTGTGG ACAGTACAGA TAATTCCTCA 
AAGCCATCCA GTGAACCCAC TTCTCACGTG GCTCGACAGC GCTTAGAAAG CACAGAGAAA 
AAGAAAATTT CTGGGAAAGT CACAAAGTCC CTCTCGGCCA GTGCTCTGTC CCTCATGATC 
CCAGGAGATA TGTTCGCTGT ATCTCCATTG GGAAGCCCAA TGTCCCCACA CTCCCTGTCT 
TCAGACCCTT CTTCTTCACG GGATTCCTCT CCCAGCCGAG ACTCTTCTGC AGCATCTGCC 
AGTCCGCATC AGCCCATTGT CATCCACAGC TCAGGCAAGA ACTATGGGTT CACCATCCGT 
GCTATCCGCG TGTACGTGGG GGACAGTGAC ATCTACACAG TGCACCATAT CGTCTGGAAC 
GTAGAAGAAG GAAGTCCCGC ATACCAGGCA GGACTGAAGG CCGGGGATCT GATCACACAC 
ATCAACGGAG AGCCGGTGCA CGGCCTCGTC CACACGGAAG TTATCGAGCT CCTGCTGAAG 
AGTGGGAATA AGGTGTCTAT CACCACTACT CCATTTGAAA ACACATCAAT CAAAACGGGA 
CCAGCCAGGA GAAACAGTTA CAAGGGCCGG ATGGTGAGAC GAAGCAAGAA GTCCAAGAAG 
AAGGAGAGTC TAGAAAGGAG GAGATCTCTC TTCAAGAAGC TGGCCAAGCA GCCTTCTCCT 
TTGCTCCACA CCAGCCGAAG TTTCTCCTGC TTAAACCGGT CCCTGTCATC TGGAGAGAGC 
CTCCCGGGTT CCCCAACTCA CAGCTTGTCC CCGAGGTCTC CAACACCCAG TTATCGTTCT 
ACTCCCGATT TTCCGTCAGG TACAAATTCC TCCCAGAGCA GCTCCCCAAG TTCAAGTGCC 
CCCAATTCTC CAGCAGGTTC AGGGCACATC CGGCCCAGCA CCCTCCATGG CCTGGCTCCC 
AAACTCAGCG GGCAGCGATA CCGCTCTGGA AGACGGAAGT CGGCTGGCAG CATCCCTCTC 
TCCCCGCTGG CCAGGACACC CTCTCCCACT CCACAGCCTA CCTCTCCTCA GCGTTCACCA 
TCCCCACTGT TGGGACACTC ACTGGGCAAT GCCAAGATCA CTCAGGCCTT TCCTAGCAAG 
ATGCACTCTC CCCCCACAAT CGTCAGACAC ATCGTGAGGC CCAAGAGTGC AGAGCCGCCC 
CGCTCCCCAC TGCTGAAACG GGTGCAGTCG GAGGAAAAGT TGTCACCCTC CTATGGCAGT 
GACAAGAAGC TTCTGTGCTC CCGCAAGCAT AGCCTAGAGG TGACACAAGA GGAGGTACAG 
AGGGAGCAGT GTCAGCGGGA AGTGACACTG CAGAGCCTGG AAGAGAATGT GTGTGACGCT 
CCTTCCCTCA GTCGGGCCAG GCCAGTGGAG CAAGGCTGTC TGAAACGCCC CGTGTCCCGG 
AAGGTGGGCA GGCAAGAGTC TGTGGATGAC CTGGACCGGG ACAAGCTGAA AGCCAAGGTT 
GTCGTAAAGA AACCAGAAGA GAAACATGAA TCGCACCAGA AACCTCACAG CCTTGGTGGT 
GATTCGGAAA GCTATGCTCT CTTCAGGCTA GAGGAGAGAG AGAAAAAAGT GTACTCCAAG 
GGGTTGGAAA GGTCAGGCCA TTTTGAAAAC ACATCAGCAG AGTTGCCTTC TGTGGGCAGC 
CTGCTGAAGG ACACTCTTCA CAAGCAGGCC AGCGTGAGGG CCAGCGAGGG GGTGACCTCA 
GACGGGGCAG CTTGCAGCCT GACACCAGGG GAGCACAGCC AGTCTCTAGG TGACTTTAAG 
CGGGCCTCAG CTTCTGGCAT TCTCCATGAT AGTGTGTGCC CCATCTCTGA TAGGCCTGCT 
CCTGGAAAGG TTGAATACTC GGAGAAGGCC TCTCAGGCCA AAGAGCTCCT TCGAAGTGAA 
AAACTAGACA GCAAGCTGGC CAATATTGAT TACCTCAGAA AGAAAATGTC ATTGGATGAC 
AAAGATGACA GCCACTGTGC CATCCTGAAA CCCAAGATAA CATCTAGCGC CCATGAATGT 
CTGCCAGGGA ACCCCATACG GCCCATGGCA GGGCAACAAG AGACCCCGCC AGCCTCTGAA 
AACCGGGCAT TCATCAACAG TACCCACACA CCTCAGATGA GTGCAGTTTC CTTTGTTCCT 
CTCAAAGCCT TAGCTGGCCG GGTAGAGAAC GGAGGGGAGA AAGCAGGCTT AGCTGCTCCC 
GAGTCCCCTG TCAGGAAGAG CCCCTCCGAG TATAAGCTAG AGGGCAGGTC AGTTTCATGT 
CTCAAGCCGA TCGAGGGCAC ACTGGACATT GCTCTCCTGT CTGGACCTCA CGCCTCCAAA 
ACAGAGTTGC TTTCCCCAGA GCCTGCACAG AGTCCCAGCC CAGGCATCAA CGTGGGACCA 
TGTGTGCCAC TAGCTCTTCC TGGGAGCAGT GGGAAAAAGG GAGACTCCAC CAGCCTGAGA 
GAGCCTTCCT CAGCCAACTT AAAAGTAAAT AAATCTTATC TGCTGGAGCC TCGGTTCCTA 
CCCCCGAGCC GGGCTCTCCA GGACTCTCTC GCAGCCTCTG GGCCAGAACC GAAGTCAAAG 
CCGGAAAGGA AGCTCATTCA TCCTTCTGCC AGGAGCCCAG CAACTGTCAC AGAGAGCAAT 
CTTCAGCAGA AAGAGGGTGG TCCCGCCACA CACCAAGACC GCTCCACTGA CACCAGGAAC 
CTCCCTGGCC CAGGGCAGAC CCTACACAAT GTGGACCTAC CCAGGCTGTG TACACGTGCC 
CCACTCCCAC CGGAAGGGAC GCCCGCAAAG GAGAAGCCAT GTCTGAAGGA ACCCTCTGCC 
AAGGTGAAAA GCGAGTGGTC TGCCGTGAGG GATGACGGAC ACAGAGATCC CTGTGCGAAG 
CTGTGCCCGG CAGAGACTGG TAAAGCCAGC GACAGTTCCA AACCCCTGCC TTCCGGGGGG 
AGGACCCAAC CCGATTTCTA CAAGCAGACC CAGACTTCGG AGAAAGCATG GGCGCATGCA 
AAAACAAACC ACAAAGATAG CCAAGATGAG GTGAAGTCCC TGGCCAGGGA GGACTCAGCT 
TCACTTTTAT ATGAAAAGGA GATAGGCCGG GCACGAAAAG GTCCTGAACC CAAACCGGAA 
GTTCCTGCTA CCCGGTGCCC TCCTCAGCCA CCAGGAATTG AGGGTGAGAA GCGAGAAAAG 
CTCTCCGCTG CCCCCTCTTT GCAGAAACAG GCTCCCAAAG AGCCAGACAG GAAGGAACAG 
ACTTCGCAAA GGCCTGGAGG TAGTGGCCCT CAACAACCCC CACCCACCAA AGAGCTGTCT 
AACTCAGCAT CCTGGCAGCA CGGCAGTTCT CCGAGTCACA CTTTAAAGAA GGAGCCCGGG 
ACCAAAGCTG CCGCTGCAGA ACCAAGCACC AGCCTTCATG ACACTCCCCG ATCTGCTACA 
GCCACCACCA CTGCCATTGC CACCACCACC ACTACCACCA GTGCCGGGCA CAGTGACTGC 
AGTAGCCATA AGGCCCGGCC TGGCCCTGAC CCCAGCCCTT CAAAGTCTAA GCACCAAGAC 
AGGTCCCTCT CCTCACAGAA GCTGAGTGCT GGCTCTGCAA AAGGCAAAGA GCCTGTCACT 
CAACCCCTGG GTGGTTCCAT CAGAGAAGGC AAGGGTGGCA GCAAGGGTCC AGTGGACACA 
TTTTCTGCTG TCCTGACCAC CCAGGGCAAA GCAAGTGATG TGCTTGTGCA GGGAGAAGGT 
CGGGTCTCAA TCATTGTCCA CACTGAAGAG TGTCCTCTCG ATGCCAAACT GAAAAACACC 
AATGGAGGGT GTCCCCCAGA GATGCAGGCG AAGCATCCAC CCAGACAAGG ACATCTCAGT 
GAAGCAGCAG ACCAGAAGCC ACTCATTGCT GGTGAGAAGC AAAGCCCGTC TCCAAAGCAT 
CCCAAACCAT CCACTGTGAA AGATTACCCC AGTCTGTGCA GACAGACAGA CAGAAGCCCA 
AGCCATCAGG CTACCACTGG GGACAGGAAG GCAGAAGGAA AGAAATGCAC AGACGCACTT 
TATGTCGCAG CCCCAGAGGG CTACAAGCCA GAGGCCAGCC CTTCTCTCCA CCACGGCGAG 
ACCGGACTCA GAGGCTCAGA GAGGCCACCC ATGGGCATGG GGAAGGGCTT CTCTGAGCCC 
AAGGGGAAAG GGCCAGGTCC CCAGAAGTCA CTGGCTGAAA CAGGCAAGCC CAGCGGTATG 
AAAAGGTCAC CCTCTGCCAC CGTGCAGAGC TCTCTCCGCT CAGCTGCCCC CCCAGAAAAG 
TCTCTGAGTT ACTCAGCCAG CTTTCCCGAG GCCCAGCCTG GAGTGCGAGA GGTCCCTGCA 
GCCAACAGCA GCCCCTCATC TGCCAAGGCT ACAGGGGGGA CCTCAGAGTT CCCAGCCCCC 
AGCAGCAGGG ACCACAGGAA GCTTCAGTCT GGAGGAGACG GCCGAAGCCA AATGATAAAG 
AGTGACTCTC TGCCCTCCTT CCGCCTCTCC ACCTCTGCTC TGGAGTCACA TTTCCAGGAT 
CCACAGGTGC CCATCGCATC AGGCCACCGA GGCAGGGCAC TGTCAGTAAC TGCTGCCACA 
GGAGAACCCA AAGGGAGAGA GCTCGCCCAG CCTCCCCCAG TCAGGAAACA GAATGCGTGC 
AGAGAGGCGA CCAGAGCACC CCCAGCCCCA AGCACAGATC GCTCCCTCCC TCTTTCCTCA 
GAGAAAGACT TCGTGGTTCG GCAGAGAAGG GGCAAGGAGA CCTTAAGGAG CAGTCCTCAC 
AAAAAGGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103167.1
CDS	475..7809
Translation	MDESSILRRRGLQEGQPPKSHFNSARHRQRLVDPAASKKELSLPRRGSLCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_030247307.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247307.1

ORF Insert Sequence:

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGG AAGGGCAACC TCCGAAGAGC 
CATTTCAACT CAGCTCGACA TCGGCAGAGA CTAGTGGACC CAGCTGCTTC AAAAAAGGAG 
CTGAGCCTCC CCCGAAGAGG AAGTCTTTGC CGAACAAGCA ACCGGAAGAG TTTGATAGGC 
AATGGCCAGT CTCCAGCACT GCCTCGACCA CACTCACCTC TCTCTGCTCA TGCAGGAAAT 
AGCCCTCAAG ACAGTCCAAG GAATTTCTCC CCCAGTGCCT CAGCCCATTT CTCATTTGCG 
AGGAGAACGG ATGGGCGCCG CTGGTCTCTG GCTTCTCTCC CTTCCTCAGG CTATGGGACA 
AATACCCCAA GCTCCACCGT CTCCTCATCC TGTTCTTCCC AGGAGAAGTT GCACCAGCTA 
CCATACCAGC CAACCCCAGA TGAATTACAC TTCTTATCCA AACACTTCTG CACAACAGAA 
AGTATCGCCA CTGAGAACCG GTGCAGAAAC ACACCCATGC GTCCACGTTC CCGGAGTCTC 
AGCCCTGGAC GGTCCCCCGC CTGCTGTGAC CATGAAATAA TTATGATGAA CCATGTCTAC 
AAAGAAAGGT TCCCAAAGGC CACAGCTCAG ATGGAAGAGC GTCTGAAGGA GATCATCACC 
AGCTACTCTC CAGACCATGT TCTCCCCTTG GCAGATGGGG TACTTAGTTT CACTCACCAT 
CAGATCATTG AGCTGGCTCG GGACTGTTTG GATAAATCTC ACCAGGGTCT CATCACGTCG 
AGATACTTTT TTGAGTTGCA GCACAAACTG GACAAGTTGC TCCAGGAGGC TCACGATCGC 
TCTGAAAGTG GAGAACTGGC ATTTATCAAG CAACTAGTCC GAAAGATCCT AATTGTCATT 
GCCCGCCCCG CTCGGTTATT GGAGTGTTTG GAATTTGATC CTGAAGAATT TTATTACCTA 
TTGGAAGCTG CAGAAGGCCA TGCCAAAGAA GGCCAAGGAA TCAAAACTGA CATCCCTAGG 
TATATCATCA GCCAGCTGGG ACTCAATAAG GACCCTTTAG AAGAAATGGC TCAGTTGGGG 
AATTACGACA GTAGGACAGC AGAGACACCA GAGATGGATG AGTCAGTGAG TAGCTCAAAT 
ACTTCCCTGA GACTTCGAAG GAAACCCCGA GAGAGTGATT TTGAAACAAT TAAATTGATC 
AGCAATGGAG CCTACGGGGC AGTCTACTTT GTTCGGCACA AAGAGTCCCG CCAGAGGTTT 
GCCATGAAGA AGATCAACAA GCAGAACCTC ATCCTTCGGA ACCAGATCCA GCAGGCCTTC 
GTGGAGCGAG ACATCCTGAC TTTCGCAGAG AACCCCTTTG TGGTCAGCAT GTATTGCTCC 
TTTGAAACGA GGCGTCACTT ATGCATGGTC ATGGAGTATG TAGAAGGGGG AGACTGTGCG 
ACCCTAATGA AAAACATGGG ACCTCTCCCA GTTGATATGG CCAGAATGTA TTTCGCCGAG 
ACCGTCTTGG CCTTGGAGTA CCTGCATAAT TACGGAATCG TACACAGGGA CTTGAAGCCA 
GACAACCTGT TGGTCACCTC CATGGGGCAC ATAAAACTGA CTGACTTCGG CTTGTCTAAG 
GTGGGATTAA TGAGCATGAC CACCAATCTC TATGAAGGCC ACATAGAGAA GGACGCTCGA 
GAGTTCTTAG ATAAACAGGT CTGTGGTACA CCTGAGTACA TTGCCCCCGA GGTGATTCTG 
AGACAGGGCT ACGGGAAACC CGTGGACTGG TGGGCCATGG GCATTATCCT CTATGAATTT 
CTGGTCGGAT GTGTGCCTTT CTTTGGGGAC ACTCCAGAAG AGCTATTTGG ACAAGTCATC 
AGTGATGAAA TCAACTGGCC TGAAAAGGAC GAGGCCCCTC CTCCAGACGC TCAGGAGCTG 
ATTACCTTGC TCCTCAGGCA GAATCCGCTG GAGAGGCTGG GAACAGGTGG AGCCTATGAA 
GTGAAGCAGC ATCGTTTCTT CCGCTCCTTA GACTGGAACA GTTTGCTGAG ACAGAAGGCG 
GAATTTATTC CCCAACTGGA ATCGGAGGAT GACACAAGTT ATTTTGATAC TCGGTCAGAG 
AAGTATCATC ACATGGAGAC GGAGGAGGAG GACGATACAA ACGATGAGGA CTTCACCGTG 
GAGATAAGGC AGTTTTCTTC CTGTTCACAC AGGTTTTCAA AAGTTTTCAG CAGTATAGAT 
CGCATAACTC AAAATTCAGG AGAAGACAAA GATGACTCTG AGGACAAGAC CAAAAGCACA 
ACGTTGCCAT CCACAGAGAC ACTCAGCTGG AGTTCCGAAT ACTCTGAAAT GCAACAGTTA 
TCGACCTCCA ACTCTTCAGA TACTGAAAGC AACAGGTGCA AACTCAGCTC TGGCTTGCTC 
CCCAAGCTGG CTATTTCGAC AGATGGGGAA CAAGATGAGG CTGTCCCTTG CTCTGGAGAC 
CCCAGAGAGG AGCCAGAGAA ACCTGTCCCT CCCTCTGAGG AGTGTACTCA GGAGGAGCCC 
GAGGTCACCA CCCCAGCCAG CACCATCAGC AGTTCCACAC TGTCAGTTGG CAGTTTTTCA 
GAGCACTTGG ATCAGATAAA TGGGCGAAGC GAGTGTGTGG ACAGTACAGA TAATTCCTCA 
AAGCCATCCA GTGAACCCAC TTCTCACGTG GCTCGACAGC GCTTAGAAAG CACAGAGAAA 
AAGAAAATTT CTGGGAAAGT CACAAAGTCC CTCTCGGCCA GTGCTCTGTC CCTCATGATC 
CCAGGAGATA TGTTCGCTGT ATCTCCATTG GGAAGCCCAA TGTCCCCACA CTCCCTGTCT 
TCAGACCCTT CTTCTTCACG GGATTCCTCT CCCAGCCGAG ACTCTTCTGC AGCATCTGCC 
AGTCCGCATC AGCCCATTGT CATCCACAGC TCAGGCAAGA ACTATGGGTT CACCATCCGT 
GCTATCCGCG TGTACGTGGG GGACAGTGAC ATCTACACAG TGCACCATAT CGTCTGGAAC 
GTAGAAGAAG GAAGTCCCGC ATACCAGGCA GGACTGAAGG CCGGGGATCT GATCACACAC 
ATCAACGGAG AGCCGGTGCA CGGCCTCGTC CACACGGAAG TTATCGAGCT CCTGCTGAAG 
AGTGGGAATA AGGTGTCTAT CACCACTACT CCATTTGAAA ACACATCAAT CAAAACGGGA 
CCAGCCAGGA GAAACAGTTA CAAGGGCCGG ATGGTGAGAC GAAGCAAGAA GTCCAAGAAG 
AAGGAGAGTC TAGAAAGGAG GAGATCTCTC TTCAAGAAGC TGGCCAAGCA GCCTTCTCCT 
TTGCTCCACA CCAGCCGAAG TTTCTCCTGC TTAAACCGGT CCCTGTCATC TGGAGAGAGC 
CTCCCGGGTT CCCCAACTCA CAGCTTGTCC CCGAGGTCTC CAACACCCAG TTATCGTTCT 
ACTCCCGATT TTCCGTCAGG TACAAATTCC TCCCAGAGCA GCTCCCCAAG TTCAAGTGCC 
CCCAATTCTC CAGCAGGTTC AGGGCACATC CGGCCCAGCA CCCTCCATGG CCTGGCTCCC 
AAACTCAGCG GGCAGCGATA CCGCTCTGGA AGACGGAAGT CGGCTGGCAG CATCCCTCTC 
TCCCCGCTGG CCAGGACACC CTCTCCCACT CCACAGCCTA CCTCTCCTCA GCGTTCACCA 
TCCCCACTGT TGGGACACTC ACTGGGCAAT GCCAAGATCA CTCAGGCCTT TCCTAGCAAG 
ATGCACTCTC CCCCCACAAT CGTCAGACAC ATCGTGAGGC CCAAGAGTGC AGAGCCGCCC 
CGCTCCCCAC TGCTGAAACG GGTGCAGTCG GAGGAAAAGT TGTCACCCTC CTATGGCAGT 
GACAAGAAGC TTCTGTGCTC CCGCAAGCAT AGCCTAGAGG TGACACAAGA GGAGGTACAG 
AGGGAGCAGT GTCAGCGGGA AGTGACACTG CAGAGCCTGG AAGAGAATGT GTGTGACGCT 
CCTTCCCTCA GTCGGGCCAG GCCAGTGGAG CAAGGCTGTC TGAAACGCCC CGTGTCCCGG 
AAGGTGGGCA GGCAAGAGTC TGTGGATGAC CTGGACCGGG ACAAGCTGAA AGCCAAGGTT 
GTCGTAAAGA AACCAGAAGA GAAACATGAA TCGCACCAGA AACCTCACAG CCTTGGTGGT 
GATTCGGAAA GCTATGCTCT CTTCAGGCTA GAGGAGAGAG AGAAAAAAGT GTACTCCAAG 
GGGTTGGAAA GGTCAGGCCA TTTTGAAAAC ACATCAGCAG AGTTGCCTTC TGTGGGCAGC 
CTGCTGAAGG ACACTCTTCA CAAGCAGGCC AGCGTGAGGG CCAGCGAGGG GGTGACCTCA 
GACGGGGCAG CTTGCAGCCT GACACCAGGG GAGCACAGCC AGTCTCTAGG TGACTTTAAG 
CGGGCCTCAG CTTCTGGCAT TCTCCATGAT AGTGTGTGCC CCATCTCTGA TAGGCCTGCT 
CCTGGAAAGG TTGAATACTC GGAGAAGGCC TCTCAGGCCA AAGAGCTCCT TCGAAGTGAA 
AAACTAGACA GCAAGCTGGC CAATATTGAT TACCTCAGAA AGAAAATGTC ATTGGATGAC 
AAAGATGACA GCCACTGTGC CATCCTGAAA CCCAAGATAA CATCTAGCGC CCATGAATGT 
CTGCCAGGGA ACCCCATACG GCCCATGGCA GGGCAACAAG AGACCCCGCC AGCCTCTGAA 
AACCGGGCAT TCATCAACAG TACCCACACA CCTCAGATGA GTGCAGTTTC CTTTGTTCCT 
CTCAAAGCCT TAGCTGGCCG GGTAGAGAAC GGAGGGGAGA AAGCAGGCTT AGCTGCTCCC 
GAGTCCCCTG TCAGGAAGAG CCCCTCCGAG TATAAGCTAG AGGGCAGGTC AGTTTCATGT 
CTCAAGCCGA TCGAGGGCAC ACTGGACATT GCTCTCCTGT CTGGACCTCA CGCCTCCAAA 
ACAGAGTTGC TTTCCCCAGA GCCTGCACAG AGTCCCAGCC CAGGCATCAA CGTGGGACCA 
TGTGTGCCAC TAGCTCTTCC TGGGAGCAGT GGGAAAAAGG GAGACTCCAC CAGCCTGAGA 
GAGCCTTCCT CAGCCAACTT AAAAGTAAAT AAATCTTATC TGCTGGAGCC TCGGTTCCTA 
CCCCCGAGCC GGGCTCTCCA GGACTCTCTC GCAGCCTCTG GGCCAGAACC GAAGTCAAAG 
CCGGAAAGGA AGCTCATTCA TCCTTCTGCC AGGAGCCCAG CAACTGTCAC AGAGAGCAAT 
CTTCAGCAGA AAGAGGGTGG TCCCGCCACA CACCAAGACC GCTCCACTGA CACCAGGAAC 
CTCCCTGGCC CAGGGCAGAC CCTACACAAT GTGGACCTAC CCAGGCTGTG TACACGTGCC 
CCACTCCCAC CGGAAGGGAC GCCCGCAAAG GAGAAGCCAT GTCTGAAGGA ACCCTCTGCC 
AAGGTGAAAA GCGAGTGGTC TGCCGTGAGG GATGACGGAC ACAGAGATCC CTGTGCGAAG 
CTGTGCCCGG CAGAGACTGG TAAAGCCAGC GACAGTTCCA AACCCCTGCC TTCCGGGGGG 
AGGACCCAAC CCGATTTCTA CAAGCAGACC CAGACTTCGG AGAAAGCATG GGCGCATGCA 
AAAACAAACC ACAAAGATAG CCAAGATGAG GTGAAGTCCC TGGCCAGGGA GGACTCAGCT 
TCACTTTTAT ATGAAAAGGA GATAGGCCGG GCACGAAAAG GTCCTGAACC CAAACCGGAA 
GTTCCTGCTA CCCGGTGCCC TCCTCAGCCA CCAGGAATTG AGGGTGAGAA GCGAGAAAAG 
CTCTCCGCTG CCCCCTCTTT GCAGAAACAG GCTCCCAAAG AGCCAGACAG GAAGGAACAG 
ACTTCGCAAA GGCCTGGAGG TAGTGGCCCT CAACAACCCC CACCCACCAA AGAGCTGTCT 
AACTCAGCAT CCTGGCAGCA CGGCAGTTCT CCGAGTCACA CTTTAAAGAA GGAGCCCGGG 
ACCAAAGCTG CCGCTGCAGA ACCAAGCACC AGCCTTCATG ACACTCCCCG ATCTGCTACA 
GCCACCACCA CTGCCATTGC CACCACCACC ACTACCACCA GTGCCGGGCA CAGTGACTGC 
AGTAGCCATA AGGCCCGGCC TGGCCCTGAC CCCAGCCCTT CAAAGTCTAA GCACCAAGAC 
AGGTCCCTCT CCTCACAGAA GCTGAGTGCT GGCTCTGCAA AAGGCAAAGA GCCTGTCACT 
CAACCCCTGG GTGGTTCCAT CAGAGAAGGC AAGGGTGGCA GCAAGGGTCC AGTGGACACA 
TTTTCTGCTG TCCTGACCAC CCAGGGCAAA GCAAGTGATG TGCTTGTGCA GGGAGAAGGT 
CGGGTCTCAA TCATTGTCCA CACTGAAGAG TGTCCTCTCG ATGCCAAACT GAAAAACACC 
AATGGAGGGT GTCCCCCAGA GATGCAGGCG AAGCATCCAC CCAGACAAGG ACATCTCAGT 
GAAGCAGCAG ACCAGAAGCC ACTCATTGCT GGTGAGAAGC AAAGCCCGTC TCCAAAGCAT 
CCCAAACCAT CCACTGTGAA AGATTACCCC AGTCTGTGCA GACAGACAGA CAGAAGCCCA 
AGCCATCAGG CTACCACTGG GGACAGGAAG GCAGAAGGAA AGAAATGCAC AGACGCACTT 
TATGTCGCAG CCCCAGAGGG CTACAAGCCA GAGGCCAGCC CTTCTCTCCA CCACGGCGAG 
ACCGGACTCA GAGGCTCAGA GAGGCCACCC ATGGGCATGG GGAAGGGCTT CTCTGAGCCC 
AAGGGGAAAG GGCCAGGTCC CCAGAAGTCA CTGGCTGAAA CAGGCAAGCC CAGCGGTATG 
AAAAGGTCAC CCTCTGCCAC CGTGCAGAGC TCTCTCCGCT CAGCTGCCCC CCCAGAAAAG 
TCTCTGAGTT ACTCAGCCAG CTTTCCCGAG GCCCAGCCTG GAGTGCGAGA GGTCCCTGCA 
GCCAACAGCA GCCCCTCATC TGCCAAGGCT ACAGGGGGGA CCTCAGAGTT CCCAGCCCCC 
AGCAGCAGGG ACCACAGGAA GCTTCAGTCT GGAGGAGACG GCCGAAGCCA AATGATAAAG 
AGTGACTCTC TGCCCTCCTT CCGCCTCTCC ACCTCTGCTC TGGAGTCACA TTTCCAGGAT 
CCACAGGTGC CCATCGCATC AGGCCACCGA GGCAGGGCAC TGTCAGTAAC TGCTGCCACA 
GGAGAACCCA AAGGGAGAGA GCTCGCCCAG CCTCCCCCAG TCAGGAAACA GAATGCGTGC 
AGAGAGGCGA CCAGAGCACC CCCAGCCCCA AGCACAGATC GCTCCCTCCC TCTTTCCTCA 
GAGAAAGACT TCGTGGTTCG GCAGAGAAGG GGCAAGGAGA CCTTAAGGAG CAGTCCTCAC 
AAAAAGGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_030247308.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103168.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X10, mRNA.

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CloneID	OMu86255
Clone ID Related Accession (Same CDS sequence)	XM_030247308.1
Accession Version	XM_030247308.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCCC AGCGGGAAAG ACTACAGATT CCGGGGCTGA CCTTGGATCT CACTCCAAGG 
AGCCTGAGTC CCACCCCATC CAGCCCGGGC AGCCCCTGTA GTCCTCTCTT GACCTTTCAC 
TTCTGGAGTT GCCGAACAAG CAACCGGAAG AGTTTGATAG GCAATGGCCA GTCTCCAGCA 
CTGCCTCGAC CACACTCACC TCTCTCTGCT CATGCAGGAA ATAGCCCTCA AGACAGTCCA 
AGGAATTTCT CCCCCAGTGC CTCAGCCCAT TTCTCATTTG CGAGGAGAAC GGATGGGCGC 
CGCTGGTCTC TGGCTTCTCT CCCTTCCTCA GGCTATGGGA CAAATACCCC AAGCTCCACC 
GTCTCCTCAT CCTGTTCTTC CCAGGAGAAG TTGCACCAGC TACCATACCA GCCAACCCCA 
GATGAATTAC ACTTCTTATC CAAACACTTC TGCACAACAG AAAGTATCGC CACTGAGAAC 
CGGTGCAGAA ACACACCCAT GCGTCCACGT TCCCGGAGTC TCAGCCCTGG ACGGTCCCCC 
GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG 
GCCACAGCTC AGATGGAAGA GCGTCTGAAG GAGATCATCA CCAGCTACTC TCCAGACCAT 
GTTCTCCCCT TGGCAGATGG GGTACTTAGT TTCACTCACC ATCAGATCAT TGAGCTGGCT 
CGGGACTGTT TGGATAAATC TCACCAGGGT CTCATCACGT CGAGATACTT TTTTGAGTTG 
CAGCACAAAC TGGACAAGTT GCTCCAGGAG GCTCACGATC GCTCTGAAAG TGGAGAACTG 
GCATTTATCA AGCAACTAGT CCGAAAGATC CTAATTGTCA TTGCCCGCCC CGCTCGGTTA 
TTGGAGTGTT TGGAATTTGA TCCTGAAGAA TTTTATTACC TATTGGAAGC TGCAGAAGGC 
CATGCCAAAG AAGGCCAAGG AATCAAAACT GACATCCCTA GGTATATCAT CAGCCAGCTG 
GGACTCAATA AGGACCCTTT AGAAGAAATG GCTCAGTTGG GGAATTACGA CAGTAGGACA 
GCAGAGACAC CAGAGATGGA TGAGTCAGTG AGTAGCTCAA ATACTTCCCT GAGACTTCGA 
AGGAAACCCC GAGAGAGTGA TTTTGAAACA ATTAAATTGA TCAGCAATGG AGCCTACGGG 
GCAGTCTACT TTGTTCGGCA CAAAGAGTCC CGCCAGAGGT TTGCCATGAA GAAGATCAAC 
AAGCAGAACC TCATCCTTCG GAACCAGATC CAGCAGGCCT TCGTGGAGCG AGACATCCTG 
ACTTTCGCAG AGAACCCCTT TGTGGTCAGC ATGTATTGCT CCTTTGAAAC GAGGCGTCAC 
TTATGCATGG TCATGGAGTA TGTAGAAGGG GGAGACTGTG CGACCCTAAT GAAAAACATG 
GGACCTCTCC CAGTTGATAT GGCCAGAATG TATTTCGCCG AGACCGTCTT GGCCTTGGAG 
TACCTGCATA ATTACGGAAT CGTACACAGG GACTTGAAGC CAGACAACCT GTTGGTCACC 
TCCATGGGGC ACATAAAACT GACTGACTTC GGCTTGTCTA AGGTGGGATT AATGAGCATG 
ACCACCAATC TCTATGAAGG CCACATAGAG AAGGACGCTC GAGAGTTCTT AGATAAACAG 
GTCTGTGGTA CACCTGAGTA CATTGCCCCC GAGGTGATTC TGAGACAGGG CTACGGGAAA 
CCCGTGGACT GGTGGGCCAT GGGCATTATC CTCTATGAAT TTCTGGTCGG ATGTGTGCCT 
TTCTTTGGGG ACACTCCAGA AGAGCTATTT GGACAAGTCA TCAGTGATGA AATCAACTGG 
CCTGAAAAGG ACGAGGCCCC TCCTCCAGAC GCTCAGGAGC TGATTACCTT GCTCCTCAGG 
CAGAATCCGC TGGAGAGGCT GGGAACAGGT GGAGCCTATG AAGTGAAGCA GCATCGTTTC 
TTCCGCTCCT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CGGAATTTAT TCCCCAACTG 
GAATCGGAGG ATGACACAAG TTATTTTGAT ACTCGGTCAG AGAAGTATCA TCACATGGAG 
ACGGAGGAGG AGGACGATAC AAACGATGAG GACTTCACCG TGGAGATAAG GCAGTTTTCT 
TCCTGTTCAC ACAGGTTTTC AAAAGTTTTC AGCAGTATAG ATCGCATAAC TCAAAATTCA 
GGAGAAGACA AAGATGACTC TGAGGACAAG ACCAAAAGCA CAACGTTGCC ATCCACAGAG 
ACACTCAGCT GGAGTTCCGA ATACTCTGAA ATGCAACAGT TATCGACCTC CAACTCTTCA 
GATACTGAAA GCAACAGGTG CAAACTCAGC TCTGGCTTGC TCCCCAAGCT GGCTATTTCG 
ACAGATGGGG AACAAGATGA GGCTGTCCCT TGCTCTGGAG ACCCCAGAGA GGAGCCAGAG 
AAACCTGTCC CTCCCTCTGA GGAGTGTACT CAGGAGGAGC CCGAGGTCAC CACCCCAGCC 
AGCACCATCA GCAGTTCCAC ACTGTCAGTT GGCAGTTTTT CAGAGCACTT GGATCAGATA 
AATGGGCGAA GCGAGTGTGT GGACAGTACA GATAATTCCT CAAAGCCATC CAGTGAACCC 
ACTTCTCACG TGGCTCGACA GCGCTTAGAA AGCACAGAGA AAAAGAAAAT TTCTGGGAAA 
GTCACAAAGT CCCTCTCGGC CAGTGCTCTG TCCCTCATGA TCCCAGGAGA TATGTTCGCT 
GTATCTCCAT TGGGAAGCCC AATGTCCCCA CACTCCCTGT CTTCAGACCC TTCTTCTTCA 
CGGGATTCCT CTCCCAGCCG AGACTCTTCT GCAGCATCTG CCAGTCCGCA TCAGCCCATT 
GTCATCCACA GCTCAGGCAA GAACTATGGG TTCACCATCC GTGCTATCCG CGTGTACGTG 
GGGGACAGTG ACATCTACAC AGTGCACCAT ATCGTCTGGA ACGTAGAAGA AGGAAGTCCC 
GCATACCAGG CAGGACTGAA GGCCGGGGAT CTGATCACAC ACATCAACGG AGAGCCGGTG 
CACGGCCTCG TCCACACGGA AGTTATCGAG CTCCTGCTGA AGAGTGGGAA TAAGGTGTCT 
ATCACCACTA CTCCATTTGA AAACACATCA ATCAAAACGG GACCAGCCAG GAGAAACAGT 
TACAAGGGCC GGATGGTGAG ACGAAGCAAG AAGTCCAAGA AGAAGGAGAG TCTAGAAAGG 
AGGAGATCTC TCTTCAAGAA GCTGGCCAAG CAGCCTTCTC CTTTGCTCCA CACCAGCCGA 
AGTTTCTCCT GCTTAAACCG GTCCCTGTCA TCTGGAGAGA GCCTCCCGGG TTCCCCAACT 
CACAGCTTGT CCCCGAGGTC TCCAACACCC AGTTATCGTT CTACTCCCGA TTTTCCGTCA 
GGTACAAATT CCTCCCAGAG CAGCTCCCCA AGTTCAAGTG CCCCCAATTC TCCAGCAGGT 
TCAGGGCACA TCCGGCCCAG CACCCTCCAT GGCCTGGCTC CCAAACTCAG CGGGCAGCGA 
TACCGCTCTG GAAGACGGAA GTCGGCTGGC AGCATCCCTC TCTCCCCGCT GGCCAGGACA 
CCCTCTCCCA CTCCACAGCC TACCTCTCCT CAGCGTTCAC CATCCCCACT GTTGGGACAC 
TCACTGGGCA ATGCCAAGAT CACTCAGGCC TTTCCTAGCA AGATGCACTC TCCCCCCACA 
ATCGTCAGAC ACATCGTGAG GCCCAAGAGT GCAGAGCCGC CCCGCTCCCC ACTGCTGAAA 
CGGGTGCAGT CGGAGGAAAA GTTGTCACCC TCCTATGGCA GTGACAAGAA GCTTCTGTGC 
TCCCGCAAGC ATAGCCTAGA GGTGACACAA GAGGAGGTAC AGAGGGAGCA GTGTCAGCGG 
GAAGTGACAC TGCAGAGCCT GGAAGAGAAT GTGTGTGACG CTCCTTCCCT CAGTCGGGCC 
AGGCCAGTGG AGCAAGGCTG TCTGAAACGC CCCGTGTCCC GGAAGGTGGG CAGGCAAGAG 
TCTGTGGATG ACCTGGACCG GGACAAGCTG AAAGCCAAGG TTGTCGTAAA GAAACCAGAA 
GAGAAACATG AATCGCACCA GAAACCTCAC AGCCTTGGTG GTGATTCGGA AAGCTATGCT 
CTCTTCAGGC TAGAGGAGAG AGAGAAAAAA GTGTACTCCA AGGGGTTGGA AAGGTCAGGC 
CATTTTGAAA ACACATCAGC AGAGTTGCCT TCTGTGGGCA GCCTGCTGAA GGACACTCTT 
CACAAGCAGG CCAGCGTGAG GGCCAGCGAG GGGGTGACCT CAGACGGGGC AGCTTGCAGC 
CTGACACCAG GGGAGCACAG CCAGTCTCTA GGTGACTTTA AGCGGGCCTC AGCTTCTGGC 
ATTCTCCATG ATAGTGTGTG CCCCATCTCT GATAGGCCTG CTCCTGGAAA GGTTGAATAC 
TCGGAGAAGG CCTCTCAGGC CAAAGAGCTC CTTCGAAGTG AAAAACTAGA CAGCAAGCTG 
GCCAATATTG ATTACCTCAG AAAGAAAATG TCATTGGATG ACAAAGATGA CAGCCACTGT 
GCCATCCTGA AACCCAAGAT AACATCTAGC GCCCATGAAT GTCTGCCAGG GAACCCCATA 
CGGCCCATGG CAGGGCAACA AGAGACCCCG CCAGCCTCTG AAAACCGGGC ATTCATCAAC 
AGTACCCACA CACCTCAGAT GAGTGCAGTT TCCTTTGTTC CTCTCAAAGC CTTAGCTGGC 
CGGGTAGAGA ACGGAGGGGA GAAAGCAGGC TTAGCTGCTC CCGAGTCCCC TGTCAGGAAG 
AGCCCCTCCG AGTATAAGCT AGAGGGCAGG TCAGTTTCAT GTCTCAAGCC GATCGAGGGC 
ACACTGGACA TTGCTCTCCT GTCTGGACCT CACGCCTCCA AAACAGAGTT GCTTTCCCCA 
GAGCCTGCAC AGAGTCCCAG CCCAGGCATC AACGTGGGAC CATGTGTGCC ACTAGCTCTT 
CCTGGGAGCA GTGGGAAAAA GGGAGACTCC ACCAGCCTGA GAGAGCCTTC CTCAGCCAAC 
TTAAAAGTAA ATAAATCTTA TCTGCTGGAG CCTCGGTTCC TACCCCCGAG CCGGGCTCTC 
CAGGACTCTC TCGCAGCCTC TGGGCCAGAA CCGAAGTCAA AGCCGGAAAG GAAGCTCATT 
CATCCTTCTG CCAGGAGCCC AGCAACTGTC ACAGAGAGCA ATCTTCAGCA GAAAGAGGGT 
GGTCCCGCCA CACACCAAGA CCGCTCCACT GACACCAGGA ACCTCCCTGG CCCAGGGCAG 
ACCCTACACA ATGTGGACCT ACCCAGGCTG TGTACACGTG CCCCACTCCC ACCGGAAGGG 
ACGCCCGCAA AGGAGAAGCC ATGTCTGAAG GAACCCTCTG CCAAGGTGAA AAGCGAGTGG 
TCTGCCGTGA GGGATGACGG ACACAGAGAT CCCTGTGCGA AGCTGTGCCC GGCAGAGACT 
GGTAAAGCCA GCGACAGTTC CAAACCCCTG CCTTCCGGGG GGAGGACCCA ACCCGATTTC 
TACAAGCAGA CCCAGACTTC GGAGAAAGCA TGGGCGCATG CAAAAACAAA CCACAAAGAT 
AGCCAAGATG AGGTGAAGTC CCTGGCCAGG GAGGACTCAG CTTCACTTTT ATATGAAAAG 
GAGATAGGCC GGGCACGAAA AGGTCCTGAA CCCAAACCGG AAGTTCCTGC TACCCGGTGC 
CCTCCTCAGC CACCAGGAAT TGAGGGTGAG AAGCGAGAAA AGCTCTCCGC TGCCCCCTCT 
TTGCAGAAAC AGGCTCCCAA AGAGCCAGAC AGGAAGGAAC AGACTTCGCA AAGGCCTGGA 
GGTAGTGGCC CTCAACAACC CCCACCCACC AAAGAGCTGT CTAACTCAGC ATCCTGGCAG 
CACGGCAGTT CTCCGAGTCA CACTTTAAAG AAGGAGCCCG GGACCAAAGC TGCCGCTGCA 
GAACCAAGCA CCAGCCTTCA TGACACTCCC CGATCTGCTA CAGCCACCAC CACTGCCATT 
GCCACCACCA CCACTACCAC CAGTGCCGGG CACAGTGACT GCAGTAGCCA TAAGGCCCGG 
CCTGGCCCTG ACCCCAGCCC TTCAAAGTCT AAGCACCAAG ACAGGTCCCT CTCCTCACAG 
AAGCTGAGTG CTGGCTCTGC AAAAGGCAAA GAGCCTGTCA CTCAACCCCT GGGTGGTTCC 
ATCAGAGAAG GCAAGGGTGG CAGCAAGGGT CCAGTGGACA CATTTTCTGC TGTCCTGACC 
ACCCAGGGCA AAGCAAGTGA TGTGCTTGTG CAGGGAGAAG GTCGGGTCTC AATCATTGTC 
CACACTGAAG AGTGTCCTCT CGATGCCAAA CTGAAAAACA CCAATGGAGG GTGTCCCCCA 
GAGATGCAGG CGAAGCATCC ACCCAGACAA GGACATCTCA GTGAAGCAGC AGACCAGAAG 
CCACTCATTG CTGGTGAGAA GCAAAGCCCG TCTCCAAAGC ATCCCAAACC ATCCACTGTG 
AAAGATTACC CCAGTCTGTG CAGACAGACA GACAGAAGCC CAAGCCATCA GGCTACCACT 
GGGGACAGGA AGGCAGAAGG AAAGAAATGC ACAGACGCAC TTTATGTCGC AGCCCCAGAG 
GGCTACAAGC CAGAGGCCAG CCCTTCTCTC CACCACGGCG AGACCGGACT CAGAGGCTCA 
GAGAGGCCAC CCATGGGCAT GGGGAAGGGC TTCTCTGAGC CCAAGGGGAA AGGGCCAGGT 
CCCCAGAAGT CACTGGCTGA AACAGGCAAG CCCAGCGGTA TGAAAAGGTC ACCCTCTGCC 
ACCGTGCAGA GCTCTCTCCG CTCAGCTGCC CCCCCAGAAA AGTCTCTGAG TTACTCAGCC 
AGCTTTCCCG AGGCCCAGCC TGGAGTGCGA GAGGTCCCTG CAGCCAACAG CAGCCCCTCA 
TCTGCCAAGG CTACAGGGGG GACCTCAGAG TTCCCAGCCC CCAGCAGCAG GGACCACAGG 
AAGCTTCAGT CTGGAGGAGA CGGCCGAAGC CAAATGATAA AGAGTGACTC TCTGCCCTCC 
TTCCGCCTCT CCACCTCTGC TCTGGAGTCA CATTTCCAGG ATCCACAGGT GCCCATCGCA 
TCAGGCCACC GAGGCAGGGC ACTGTCAGTA ACTGCTGCCA CAGGAGAACC CAAAGGGAGA 
GAGCTCGCCC AGCCTCCCCC AGTCAGGAAA CAGAATGCGT GCAGAGAGGC GACCAGAGCA 
CCCCCAGCCC CAAGCACAGA TCGCTCCCTC CCTCTTTCCT CAGAGAAAGA CTTCGTGGTT 
CGGCAGAGAA GGGGCAAGGA GACCTTAAGG AGCAGTCCTC ACAAAAAGGC CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103168.1
CDS	257..7573
Translation	MKAQRERLQIPGLTLDLTPRSLSPTPSSPGSPCSPLLTFHFWSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_030247308.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247308.1

ORF Insert Sequence:

ATGAAAGCCC AGCGGGAAAG ACTACAGATT CCGGGGCTGA CCTTGGATCT CACTCCAAGG 
AGCCTGAGTC CCACCCCATC CAGCCCGGGC AGCCCCTGTA GTCCTCTCTT GACCTTTCAC 
TTCTGGAGTT GCCGAACAAG CAACCGGAAG AGTTTGATAG GCAATGGCCA GTCTCCAGCA 
CTGCCTCGAC CACACTCACC TCTCTCTGCT CATGCAGGAA ATAGCCCTCA AGACAGTCCA 
AGGAATTTCT CCCCCAGTGC CTCAGCCCAT TTCTCATTTG CGAGGAGAAC GGATGGGCGC 
CGCTGGTCTC TGGCTTCTCT CCCTTCCTCA GGCTATGGGA CAAATACCCC AAGCTCCACC 
GTCTCCTCAT CCTGTTCTTC CCAGGAGAAG TTGCACCAGC TACCATACCA GCCAACCCCA 
GATGAATTAC ACTTCTTATC CAAACACTTC TGCACAACAG AAAGTATCGC CACTGAGAAC 
CGGTGCAGAA ACACACCCAT GCGTCCACGT TCCCGGAGTC TCAGCCCTGG ACGGTCCCCC 
GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG 
GCCACAGCTC AGATGGAAGA GCGTCTGAAG GAGATCATCA CCAGCTACTC TCCAGACCAT 
GTTCTCCCCT TGGCAGATGG GGTACTTAGT TTCACTCACC ATCAGATCAT TGAGCTGGCT 
CGGGACTGTT TGGATAAATC TCACCAGGGT CTCATCACGT CGAGATACTT TTTTGAGTTG 
CAGCACAAAC TGGACAAGTT GCTCCAGGAG GCTCACGATC GCTCTGAAAG TGGAGAACTG 
GCATTTATCA AGCAACTAGT CCGAAAGATC CTAATTGTCA TTGCCCGCCC CGCTCGGTTA 
TTGGAGTGTT TGGAATTTGA TCCTGAAGAA TTTTATTACC TATTGGAAGC TGCAGAAGGC 
CATGCCAAAG AAGGCCAAGG AATCAAAACT GACATCCCTA GGTATATCAT CAGCCAGCTG 
GGACTCAATA AGGACCCTTT AGAAGAAATG GCTCAGTTGG GGAATTACGA CAGTAGGACA 
GCAGAGACAC CAGAGATGGA TGAGTCAGTG AGTAGCTCAA ATACTTCCCT GAGACTTCGA 
AGGAAACCCC GAGAGAGTGA TTTTGAAACA ATTAAATTGA TCAGCAATGG AGCCTACGGG 
GCAGTCTACT TTGTTCGGCA CAAAGAGTCC CGCCAGAGGT TTGCCATGAA GAAGATCAAC 
AAGCAGAACC TCATCCTTCG GAACCAGATC CAGCAGGCCT TCGTGGAGCG AGACATCCTG 
ACTTTCGCAG AGAACCCCTT TGTGGTCAGC ATGTATTGCT CCTTTGAAAC GAGGCGTCAC 
TTATGCATGG TCATGGAGTA TGTAGAAGGG GGAGACTGTG CGACCCTAAT GAAAAACATG 
GGACCTCTCC CAGTTGATAT GGCCAGAATG TATTTCGCCG AGACCGTCTT GGCCTTGGAG 
TACCTGCATA ATTACGGAAT CGTACACAGG GACTTGAAGC CAGACAACCT GTTGGTCACC 
TCCATGGGGC ACATAAAACT GACTGACTTC GGCTTGTCTA AGGTGGGATT AATGAGCATG 
ACCACCAATC TCTATGAAGG CCACATAGAG AAGGACGCTC GAGAGTTCTT AGATAAACAG 
GTCTGTGGTA CACCTGAGTA CATTGCCCCC GAGGTGATTC TGAGACAGGG CTACGGGAAA 
CCCGTGGACT GGTGGGCCAT GGGCATTATC CTCTATGAAT TTCTGGTCGG ATGTGTGCCT 
TTCTTTGGGG ACACTCCAGA AGAGCTATTT GGACAAGTCA TCAGTGATGA AATCAACTGG 
CCTGAAAAGG ACGAGGCCCC TCCTCCAGAC GCTCAGGAGC TGATTACCTT GCTCCTCAGG 
CAGAATCCGC TGGAGAGGCT GGGAACAGGT GGAGCCTATG AAGTGAAGCA GCATCGTTTC 
TTCCGCTCCT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CGGAATTTAT TCCCCAACTG 
GAATCGGAGG ATGACACAAG TTATTTTGAT ACTCGGTCAG AGAAGTATCA TCACATGGAG 
ACGGAGGAGG AGGACGATAC AAACGATGAG GACTTCACCG TGGAGATAAG GCAGTTTTCT 
TCCTGTTCAC ACAGGTTTTC AAAAGTTTTC AGCAGTATAG ATCGCATAAC TCAAAATTCA 
GGAGAAGACA AAGATGACTC TGAGGACAAG ACCAAAAGCA CAACGTTGCC ATCCACAGAG 
ACACTCAGCT GGAGTTCCGA ATACTCTGAA ATGCAACAGT TATCGACCTC CAACTCTTCA 
GATACTGAAA GCAACAGGTG CAAACTCAGC TCTGGCTTGC TCCCCAAGCT GGCTATTTCG 
ACAGATGGGG AACAAGATGA GGCTGTCCCT TGCTCTGGAG ACCCCAGAGA GGAGCCAGAG 
AAACCTGTCC CTCCCTCTGA GGAGTGTACT CAGGAGGAGC CCGAGGTCAC CACCCCAGCC 
AGCACCATCA GCAGTTCCAC ACTGTCAGTT GGCAGTTTTT CAGAGCACTT GGATCAGATA 
AATGGGCGAA GCGAGTGTGT GGACAGTACA GATAATTCCT CAAAGCCATC CAGTGAACCC 
ACTTCTCACG TGGCTCGACA GCGCTTAGAA AGCACAGAGA AAAAGAAAAT TTCTGGGAAA 
GTCACAAAGT CCCTCTCGGC CAGTGCTCTG TCCCTCATGA TCCCAGGAGA TATGTTCGCT 
GTATCTCCAT TGGGAAGCCC AATGTCCCCA CACTCCCTGT CTTCAGACCC TTCTTCTTCA 
CGGGATTCCT CTCCCAGCCG AGACTCTTCT GCAGCATCTG CCAGTCCGCA TCAGCCCATT 
GTCATCCACA GCTCAGGCAA GAACTATGGG TTCACCATCC GTGCTATCCG CGTGTACGTG 
GGGGACAGTG ACATCTACAC AGTGCACCAT ATCGTCTGGA ACGTAGAAGA AGGAAGTCCC 
GCATACCAGG CAGGACTGAA GGCCGGGGAT CTGATCACAC ACATCAACGG AGAGCCGGTG 
CACGGCCTCG TCCACACGGA AGTTATCGAG CTCCTGCTGA AGAGTGGGAA TAAGGTGTCT 
ATCACCACTA CTCCATTTGA AAACACATCA ATCAAAACGG GACCAGCCAG GAGAAACAGT 
TACAAGGGCC GGATGGTGAG ACGAAGCAAG AAGTCCAAGA AGAAGGAGAG TCTAGAAAGG 
AGGAGATCTC TCTTCAAGAA GCTGGCCAAG CAGCCTTCTC CTTTGCTCCA CACCAGCCGA 
AGTTTCTCCT GCTTAAACCG GTCCCTGTCA TCTGGAGAGA GCCTCCCGGG TTCCCCAACT 
CACAGCTTGT CCCCGAGGTC TCCAACACCC AGTTATCGTT CTACTCCCGA TTTTCCGTCA 
GGTACAAATT CCTCCCAGAG CAGCTCCCCA AGTTCAAGTG CCCCCAATTC TCCAGCAGGT 
TCAGGGCACA TCCGGCCCAG CACCCTCCAT GGCCTGGCTC CCAAACTCAG CGGGCAGCGA 
TACCGCTCTG GAAGACGGAA GTCGGCTGGC AGCATCCCTC TCTCCCCGCT GGCCAGGACA 
CCCTCTCCCA CTCCACAGCC TACCTCTCCT CAGCGTTCAC CATCCCCACT GTTGGGACAC 
TCACTGGGCA ATGCCAAGAT CACTCAGGCC TTTCCTAGCA AGATGCACTC TCCCCCCACA 
ATCGTCAGAC ACATCGTGAG GCCCAAGAGT GCAGAGCCGC CCCGCTCCCC ACTGCTGAAA 
CGGGTGCAGT CGGAGGAAAA GTTGTCACCC TCCTATGGCA GTGACAAGAA GCTTCTGTGC 
TCCCGCAAGC ATAGCCTAGA GGTGACACAA GAGGAGGTAC AGAGGGAGCA GTGTCAGCGG 
GAAGTGACAC TGCAGAGCCT GGAAGAGAAT GTGTGTGACG CTCCTTCCCT CAGTCGGGCC 
AGGCCAGTGG AGCAAGGCTG TCTGAAACGC CCCGTGTCCC GGAAGGTGGG CAGGCAAGAG 
TCTGTGGATG ACCTGGACCG GGACAAGCTG AAAGCCAAGG TTGTCGTAAA GAAACCAGAA 
GAGAAACATG AATCGCACCA GAAACCTCAC AGCCTTGGTG GTGATTCGGA AAGCTATGCT 
CTCTTCAGGC TAGAGGAGAG AGAGAAAAAA GTGTACTCCA AGGGGTTGGA AAGGTCAGGC 
CATTTTGAAA ACACATCAGC AGAGTTGCCT TCTGTGGGCA GCCTGCTGAA GGACACTCTT 
CACAAGCAGG CCAGCGTGAG GGCCAGCGAG GGGGTGACCT CAGACGGGGC AGCTTGCAGC 
CTGACACCAG GGGAGCACAG CCAGTCTCTA GGTGACTTTA AGCGGGCCTC AGCTTCTGGC 
ATTCTCCATG ATAGTGTGTG CCCCATCTCT GATAGGCCTG CTCCTGGAAA GGTTGAATAC 
TCGGAGAAGG CCTCTCAGGC CAAAGAGCTC CTTCGAAGTG AAAAACTAGA CAGCAAGCTG 
GCCAATATTG ATTACCTCAG AAAGAAAATG TCATTGGATG ACAAAGATGA CAGCCACTGT 
GCCATCCTGA AACCCAAGAT AACATCTAGC GCCCATGAAT GTCTGCCAGG GAACCCCATA 
CGGCCCATGG CAGGGCAACA AGAGACCCCG CCAGCCTCTG AAAACCGGGC ATTCATCAAC 
AGTACCCACA CACCTCAGAT GAGTGCAGTT TCCTTTGTTC CTCTCAAAGC CTTAGCTGGC 
CGGGTAGAGA ACGGAGGGGA GAAAGCAGGC TTAGCTGCTC CCGAGTCCCC TGTCAGGAAG 
AGCCCCTCCG AGTATAAGCT AGAGGGCAGG TCAGTTTCAT GTCTCAAGCC GATCGAGGGC 
ACACTGGACA TTGCTCTCCT GTCTGGACCT CACGCCTCCA AAACAGAGTT GCTTTCCCCA 
GAGCCTGCAC AGAGTCCCAG CCCAGGCATC AACGTGGGAC CATGTGTGCC ACTAGCTCTT 
CCTGGGAGCA GTGGGAAAAA GGGAGACTCC ACCAGCCTGA GAGAGCCTTC CTCAGCCAAC 
TTAAAAGTAA ATAAATCTTA TCTGCTGGAG CCTCGGTTCC TACCCCCGAG CCGGGCTCTC 
CAGGACTCTC TCGCAGCCTC TGGGCCAGAA CCGAAGTCAA AGCCGGAAAG GAAGCTCATT 
CATCCTTCTG CCAGGAGCCC AGCAACTGTC ACAGAGAGCA ATCTTCAGCA GAAAGAGGGT 
GGTCCCGCCA CACACCAAGA CCGCTCCACT GACACCAGGA ACCTCCCTGG CCCAGGGCAG 
ACCCTACACA ATGTGGACCT ACCCAGGCTG TGTACACGTG CCCCACTCCC ACCGGAAGGG 
ACGCCCGCAA AGGAGAAGCC ATGTCTGAAG GAACCCTCTG CCAAGGTGAA AAGCGAGTGG 
TCTGCCGTGA GGGATGACGG ACACAGAGAT CCCTGTGCGA AGCTGTGCCC GGCAGAGACT 
GGTAAAGCCA GCGACAGTTC CAAACCCCTG CCTTCCGGGG GGAGGACCCA ACCCGATTTC 
TACAAGCAGA CCCAGACTTC GGAGAAAGCA TGGGCGCATG CAAAAACAAA CCACAAAGAT 
AGCCAAGATG AGGTGAAGTC CCTGGCCAGG GAGGACTCAG CTTCACTTTT ATATGAAAAG 
GAGATAGGCC GGGCACGAAA AGGTCCTGAA CCCAAACCGG AAGTTCCTGC TACCCGGTGC 
CCTCCTCAGC CACCAGGAAT TGAGGGTGAG AAGCGAGAAA AGCTCTCCGC TGCCCCCTCT 
TTGCAGAAAC AGGCTCCCAA AGAGCCAGAC AGGAAGGAAC AGACTTCGCA AAGGCCTGGA 
GGTAGTGGCC CTCAACAACC CCCACCCACC AAAGAGCTGT CTAACTCAGC ATCCTGGCAG 
CACGGCAGTT CTCCGAGTCA CACTTTAAAG AAGGAGCCCG GGACCAAAGC TGCCGCTGCA 
GAACCAAGCA CCAGCCTTCA TGACACTCCC CGATCTGCTA CAGCCACCAC CACTGCCATT 
GCCACCACCA CCACTACCAC CAGTGCCGGG CACAGTGACT GCAGTAGCCA TAAGGCCCGG 
CCTGGCCCTG ACCCCAGCCC TTCAAAGTCT AAGCACCAAG ACAGGTCCCT CTCCTCACAG 
AAGCTGAGTG CTGGCTCTGC AAAAGGCAAA GAGCCTGTCA CTCAACCCCT GGGTGGTTCC 
ATCAGAGAAG GCAAGGGTGG CAGCAAGGGT CCAGTGGACA CATTTTCTGC TGTCCTGACC 
ACCCAGGGCA AAGCAAGTGA TGTGCTTGTG CAGGGAGAAG GTCGGGTCTC AATCATTGTC 
CACACTGAAG AGTGTCCTCT CGATGCCAAA CTGAAAAACA CCAATGGAGG GTGTCCCCCA 
GAGATGCAGG CGAAGCATCC ACCCAGACAA GGACATCTCA GTGAAGCAGC AGACCAGAAG 
CCACTCATTG CTGGTGAGAA GCAAAGCCCG TCTCCAAAGC ATCCCAAACC ATCCACTGTG 
AAAGATTACC CCAGTCTGTG CAGACAGACA GACAGAAGCC CAAGCCATCA GGCTACCACT 
GGGGACAGGA AGGCAGAAGG AAAGAAATGC ACAGACGCAC TTTATGTCGC AGCCCCAGAG 
GGCTACAAGC CAGAGGCCAG CCCTTCTCTC CACCACGGCG AGACCGGACT CAGAGGCTCA 
GAGAGGCCAC CCATGGGCAT GGGGAAGGGC TTCTCTGAGC CCAAGGGGAA AGGGCCAGGT 
CCCCAGAAGT CACTGGCTGA AACAGGCAAG CCCAGCGGTA TGAAAAGGTC ACCCTCTGCC 
ACCGTGCAGA GCTCTCTCCG CTCAGCTGCC CCCCCAGAAA AGTCTCTGAG TTACTCAGCC 
AGCTTTCCCG AGGCCCAGCC TGGAGTGCGA GAGGTCCCTG CAGCCAACAG CAGCCCCTCA 
TCTGCCAAGG CTACAGGGGG GACCTCAGAG TTCCCAGCCC CCAGCAGCAG GGACCACAGG 
AAGCTTCAGT CTGGAGGAGA CGGCCGAAGC CAAATGATAA AGAGTGACTC TCTGCCCTCC 
TTCCGCCTCT CCACCTCTGC TCTGGAGTCA CATTTCCAGG ATCCACAGGT GCCCATCGCA 
TCAGGCCACC GAGGCAGGGC ACTGTCAGTA ACTGCTGCCA CAGGAGAACC CAAAGGGAGA 
GAGCTCGCCC AGCCTCCCCC AGTCAGGAAA CAGAATGCGT GCAGAGAGGC GACCAGAGCA 
CCCCCAGCCC CAAGCACAGA TCGCTCCCTC CCTCTTTCCT CAGAGAAAGA CTTCGTGGTT 
CGGCAGAGAA GGGGCAAGGA GACCTTAAGG AGCAGTCCTC ACAAAAAGGC CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_030247309.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103169.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X12, mRNA.

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CloneID	OMu86256
Clone ID Related Accession (Same CDS sequence)	XM_030247309.1
Accession Version	XM_030247309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGA AGGAGCTGAG CCTCCCCCGA 
AGAGGAAGTC TGGTAGATTC CCAGAAGTGG AATTGCTTGG TCAAACGTTG CCGAACAAGC 
AACCGGAAGA GTTTGATAGG CAATGGCCAG TCTCCAGCAC TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GACAGTCCAA GGAATTTCTC CCCCAGTGCC 
TCAGCCCATT TCTCATTTGC GAGGAGAACG GATGGGCGCC GCTGGTCTCT GGCTTCTCTC 
CCTTCCTCAG GCTATGGGAC AAATACCCCA AGCTCCACCG TCTCCTCATC CTGTTCTTCC 
CAGGAGAAGT TGCACCAGCT ACCATACCAG CCAACCCCAG ATGAATTACA CTTCTTATCC 
AAACACTTCT GCACAACAGA AAGTATCGCC ACTGAGAACC GGTGCAGAAA CACACCCATG 
CGTCCACGTT CCCGGAGTCT CAGCCCTGGA CGGTCCCCCG CCTGCTGTGA CCATGAAATA 
ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CCACAGCTCA GATGGAAGAG 
CGTCTGAAGG AGATCATCAC CAGCTACTCT CCAGACCATG TTCTCCCCTT GGCAGATGGG 
GTACTTAGTT TCACTCACCA TCAGATCATT GAGCTGGCTC GGGACTGTTT GGATAAATCT 
CACCAGGGTC TCATCACGTC GAGATACTTT TTTGAGTTGC AGCACAAACT GGACAAGTTG 
CTCCAGGAGG CTCACGATCG CTCTGAAAGT GGAGAACTGG CATTTATCAA GCAACTAGTC 
CGAAAGATCC TAATTGTCAT TGCCCGCCCC GCTCGGTTAT TGGAGTGTTT GGAATTTGAT 
CCTGAAGAAT TTTATTACCT ATTGGAAGCT GCAGAAGGCC ATGCCAAAGA AGGCCAAGGA 
ATCAAAACTG ACATCCCTAG GTATATCATC AGCCAGCTGG GACTCAATAA GGACCCTTTA 
GAAGAAATGG CTCAGTTGGG GAATTACGAC AGTAGGACAG CAGAGACACC AGAGATGGAT 
GAGTCAGTGA GTAGCTCAAA TACTTCCCTG AGACTTCGAA GGAAACCCCG AGAGAGTGAT 
TTTGAAACAA TTAAATTGAT CAGCAATGGA GCCTACGGGG CAGTCTACTT TGTTCGGCAC 
AAAGAGTCCC GCCAGAGGTT TGCCATGAAG AAGATCAACA AGCAGAACCT CATCCTTCGG 
AACCAGATCC AGCAGGCCTT CGTGGAGCGA GACATCCTGA CTTTCGCAGA GAACCCCTTT 
GTGGTCAGCA TGTATTGCTC CTTTGAAACG AGGCGTCACT TATGCATGGT CATGGAGTAT 
GTAGAAGGGG GAGACTGTGC GACCCTAATG AAAAACATGG GACCTCTCCC AGTTGATATG 
GCCAGAATGT ATTTCGCCGA GACCGTCTTG GCCTTGGAGT ACCTGCATAA TTACGGAATC 
GTACACAGGG ACTTGAAGCC AGACAACCTG TTGGTCACCT CCATGGGGCA CATAAAACTG 
ACTGACTTCG GCTTGTCTAA GGTGGGATTA ATGAGCATGA CCACCAATCT CTATGAAGGC 
CACATAGAGA AGGACGCTCG AGAGTTCTTA GATAAACAGG TCTGTGGTAC ACCTGAGTAC 
ATTGCCCCCG AGGTGATTCT GAGACAGGGC TACGGGAAAC CCGTGGACTG GTGGGCCATG 
GGCATTATCC TCTATGAATT TCTGGTCGGA TGTGTGCCTT TCTTTGGGGA CACTCCAGAA 
GAGCTATTTG GACAAGTCAT CAGTGATGAA ATCAACTGGC CTGAAAAGGA CGAGGCCCCT 
CCTCCAGACG CTCAGGAGCT GATTACCTTG CTCCTCAGGC AGAATCCGCT GGAGAGGCTG 
GGAACAGGTG GAGCCTATGA AGTGAAGCAG CATCGTTTCT TCCGCTCCTT AGACTGGAAC 
AGTTTGCTGA GACAGAAGGC GGAATTTATT CCCCAACTGG AATCGGAGGA TGACACAAGT 
TATTTTGATA CTCGGTCAGA GAAGTATCAT CACATGGAGA CGGAGGAGGA GGACGATACA 
AACGATGAGG ACTTCACCGT GGAGATAAGG CAGTTTTCTT CCTGTTCACA CAGGTTTTCA 
AAAGTTTTCA GCAGTATAGA TCGCATAACT CAAAATTCAG GAGAAGACAA AGATGACTCT 
GAGGACAAGA CCAAAAGCAC AACGTTGCCA TCCACAGAGA CACTCAGCTG GAGTTCCGAA 
TACTCTGAAA TGCAACAGTT ATCGACCTCC AACTCTTCAG ATACTGAAAG CAACAGGTGC 
AAACTCAGCT CTGGCTTGCT CCCCAAGCTG GCTATTTCGA CAGATGGGGA ACAAGATGAG 
GCTGTCCCTT GCTCTGGAGA CCCCAGAGAG GAGCCAGAGA AACCTGTCCC TCCCTCTGAG 
GAGTGTACTC AGGAGGAGCC CGAGGTCACC ACCCCAGCCA GCACCATCAG CAGTTCCACA 
CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGGCGAAG CGAGTGTGTG 
GACAGTACAG ATAATTCCTC AAAGCCATCC AGTGAACCCA CTTCTCACGT GGCTCGACAG 
CGCTTAGAAA GCACAGAGAA AAAGAAAATT TCTGGGAAAG TCACAAAGTC CCTCTCGGCC 
AGTGCTCTGT CCCTCATGAT CCCAGGAGAT ATGTTCGCTG TATCTCCATT GGGAAGCCCA 
ATGTCCCCAC ACTCCCTGTC TTCAGACCCT TCTTCTTCAC GGGATTCCTC TCCCAGCCGA 
GACTCTTCTG CAGCATCTGC CAGTCCGCAT CAGCCCATTG TCATCCACAG CTCAGGCAAG 
AACTATGGGT TCACCATCCG TGCTATCCGC GTGTACGTGG GGGACAGTGA CATCTACACA 
GTGCACCATA TCGTCTGGAA CGTAGAAGAA GGAAGTCCCG CATACCAGGC AGGACTGAAG 
GCCGGGGATC TGATCACACA CATCAACGGA GAGCCGGTGC ACGGCCTCGT CCACACGGAA 
GTTATCGAGC TCCTGCTGAA GAGTGGGAAT AAGGTGTCTA TCACCACTAC TCCATTTGAA 
AACACATCAA TCAAAACGGG ACCAGCCAGG AGAAACAGTT ACAAGGGCCG GATGGTGAGA 
CGAAGCAAGA AGTCCAAGAA GAAGGAGAGT CTAGAAAGGA GGAGATCTCT CTTCAAGAAG 
CTGGCCAAGC AGCCTTCTCC TTTGCTCCAC ACCAGCCGAA GTTTCTCCTG CTTAAACCGG 
TCCCTGTCAT CTGGAGAGAG CCTCCCGGGT TCCCCAACTC ACAGCTTGTC CCCGAGGTCT 
CCAACACCCA GTTATCGTTC TACTCCCGAT TTTCCGTCAG GTACAAATTC CTCCCAGAGC 
AGCTCCCCAA GTTCAAGTGC CCCCAATTCT CCAGCAGGTT CAGGGCACAT CCGGCCCAGC 
ACCCTCCATG GCCTGGCTCC CAAACTCAGC GGGCAGCGAT ACCGCTCTGG AAGACGGAAG 
TCGGCTGGCA GCATCCCTCT CTCCCCGCTG GCCAGGACAC CCTCTCCCAC TCCACAGCCT 
ACCTCTCCTC AGCGTTCACC ATCCCCACTG TTGGGACACT CACTGGGCAA TGCCAAGATC 
ACTCAGGCCT TTCCTAGCAA GATGCACTCT CCCCCCACAA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CAGAGCCGCC CCGCTCCCCA CTGCTGAAAC GGGTGCAGTC GGAGGAAAAG 
TTGTCACCCT CCTATGGCAG TGACAAGAAG CTTCTGTGCT CCCGCAAGCA TAGCCTAGAG 
GTGACACAAG AGGAGGTACA GAGGGAGCAG TGTCAGCGGG AAGTGACACT GCAGAGCCTG 
GAAGAGAATG TGTGTGACGC TCCTTCCCTC AGTCGGGCCA GGCCAGTGGA GCAAGGCTGT 
CTGAAACGCC CCGTGTCCCG GAAGGTGGGC AGGCAAGAGT CTGTGGATGA CCTGGACCGG 
GACAAGCTGA AAGCCAAGGT TGTCGTAAAG AAACCAGAAG AGAAACATGA ATCGCACCAG 
AAACCTCACA GCCTTGGTGG TGATTCGGAA AGCTATGCTC TCTTCAGGCT AGAGGAGAGA 
GAGAAAAAAG TGTACTCCAA GGGGTTGGAA AGGTCAGGCC ATTTTGAAAA CACATCAGCA 
GAGTTGCCTT CTGTGGGCAG CCTGCTGAAG GACACTCTTC ACAAGCAGGC CAGCGTGAGG 
GCCAGCGAGG GGGTGACCTC AGACGGGGCA GCTTGCAGCC TGACACCAGG GGAGCACAGC 
CAGTCTCTAG GTGACTTTAA GCGGGCCTCA GCTTCTGGCA TTCTCCATGA TAGTGTGTGC 
CCCATCTCTG ATAGGCCTGC TCCTGGAAAG GTTGAATACT CGGAGAAGGC CTCTCAGGCC 
AAAGAGCTCC TTCGAAGTGA AAAACTAGAC AGCAAGCTGG CCAATATTGA TTACCTCAGA 
AAGAAAATGT CATTGGATGA CAAAGATGAC AGCCACTGTG CCATCCTGAA ACCCAAGATA 
ACATCTAGCG CCCATGAATG TCTGCCAGGG AACCCCATAC GGCCCATGGC AGGGCAACAA 
GAGACCCCGC CAGCCTCTGA AAACCGGGCA TTCATCAACA GTACCCACAC ACCTCAGATG 
AGTGCAGTTT CCTTTGTTCC TCTCAAAGCC TTAGCTGGCC GGGTAGAGAA CGGAGGGGAG 
AAAGCAGGCT TAGCTGCTCC CGAGTCCCCT GTCAGGAAGA GCCCCTCCGA GTATAAGCTA 
GAGGGCAGGT CAGTTTCATG TCTCAAGCCG ATCGAGGGCA CACTGGACAT TGCTCTCCTG 
TCTGGACCTC ACGCCTCCAA AACAGAGTTG CTTTCCCCAG AGCCTGCACA GAGTCCCAGC 
CCAGGCATCA ACGTGGGACC ATGTGTGCCA CTAGCTCTTC CTGGGAGCAG TGGGAAAAAG 
GGAGACTCCA CCAGCCTGAG AGAGCCTTCC TCAGCCAACT TAAAAGTAAA TAAATCTTAT 
CTGCTGGAGC CTCGGTTCCT ACCCCCGAGC CGGGCTCTCC AGGACTCTCT CGCAGCCTCT 
GGGCCAGAAC CGAAGTCAAA GCCGGAAAGG AAGCTCATTC ATCCTTCTGC CAGGAGCCCA 
GCAACTGTCA CAGAGAGCAA TCTTCAGCAG AAAGAGGGTG GTCCCGCCAC ACACCAAGAC 
CGCTCCACTG ACACCAGGAA CCTCCCTGGC CCAGGGCAGA CCCTACACAA TGTGGACCTA 
CCCAGGCTGT GTACACGTGC CCCACTCCCA CCGGAAGGGA CGCCCGCAAA GGAGAAGCCA 
TGTCTGAAGG AACCCTCTGC CAAGGTGAAA AGCGAGTGGT CTGCCGTGAG GGATGACGGA 
CACAGAGATC CCTGTGCGAA GCTGTGCCCG GCAGAGACTG GTAAAGCCAG CGACAGTTCC 
AAACCCCTGC CTTCCGGGGG GAGGACCCAA CCCGATTTCT ACAAGCAGAC CCAGACTTCG 
GAGAAAGCAT GGGCGCATGC AAAAACAAAC CACAAAGATA GCCAAGATGA GGTGAAGTCC 
CTGGCCAGGG AGGACTCAGC TTCACTTTTA TATGAAAAGG AGATAGGCCG GGCACGAAAA 
GGTCCTGAAC CCAAACCGGA AGTTCCTGCT ACCCGGTGCC CTCCTCAGCC ACCAGGAATT 
GAGGGTGAGA AGCGAGAAAA GCTCTCCGCT GCCCCCTCTT TGCAGAAACA GGCTCCCAAA 
GAGCCAGACA GGAAGGAACA GACTTCGCAA AGGCCTGGAG GTAGTGGCCC TCAACAACCC 
CCACCCACCA AAGAGCTGTC TAACTCAGCA TCCTGGCAGC ACGGCAGTTC TCCGAGTCAC 
ACTTTAAAGA AGGAGCCCGG GACCAAAGCT GCCGCTGCAG AACCAAGCAC CAGCCTTCAT 
GACACTCCCC GATCTGCTAC AGCCACCACC ACTGCCATTG CCACCACCAC CACTACCACC 
AGTGCCGGGC ACAGTGACTG CAGTAGCCAT AAGGCCCGGC CTGGCCCTGA CCCCAGCCCT 
TCAAAGTCTA AGCACCAAGA CAGGTCCCTC TCCTCACAGA AGCTGAGTGC TGGCTCTGCA 
AAAGGCAAAG AGCCTGTCAC TCAACCCCTG GGTGGTTCCA TCAGAGAAGG CAAGGGTGGC 
AGCAAGGGTC CAGTGGACAC ATTTTCTGCT GTCCTGACCA CCCAGGGCAA AGCAAGTGAT 
GTGCTTGTGC AGGGAGAAGG TCGGGTCTCA ATCATTGTCC ACACTGAAGA GTGTCCTCTC 
GATGCCAAAC TGAAAAACAC CAATGGAGGG TGTCCCCCAG AGATGCAGGC GAAGCATCCA 
CCCAGACAAG GACATCTCAG TGAAGCAGCA GACCAGAAGC CACTCATTGC TGGTGAGAAG 
CAAAGCCCGT CTCCAAAGCA TCCCAAACCA TCCACTGTGA AAGATTACCC CAGTCTGTGC 
AGACAGACAG ACAGAAGCCC AAGCCATCAG GCTACCACTG GGGACAGGAA GGCAGAAGGA 
AAGAAATGCA CAGACGCACT TTATGTCGCA GCCCCAGAGG GCTACAAGCC AGAGGCCAGC 
CCTTCTCTCC ACCACGGCGA GACCGGACTC AGAGGCTCAG AGAGGCCACC CATGGGCATG 
GGGAAGGGCT TCTCTGAGCC CAAGGGGAAA GGGCCAGGTC CCCAGAAGTC ACTGGCTGAA 
ACAGGCAAGC CCAGCGGTAT GAAAAGGTCA CCCTCTGCCA CCGTGCAGAG CTCTCTCCGC 
TCAGCTGCCC CCCCAGAAAA GTCTCTGAGT TACTCAGCCA GCTTTCCCGA GGCCCAGCCT 
GGAGTGCGAG AGGTCCCTGC AGCCAACAGC AGCCCCTCAT CTGCCAAGGC TACAGGGGGG 
ACCTCAGAGT TCCCAGCCCC CAGCAGCAGG GACCACAGGA AGCTTCAGTC TGGAGGAGAC 
GGCCGAAGCC AAATGATAAA GAGTGACTCT CTGCCCTCCT TCCGCCTCTC CACCTCTGCT 
CTGGAGTCAC ATTTCCAGGA TCCACAGGTG CCCATCGCAT CAGGCCACCG AGGCAGGGCA 
CTGTCAGTAA CTGCTGCCAC AGGAGAACCC AAAGGGAGAG AGCTCGCCCA GCCTCCCCCA 
GTCAGGAAAC AGAATGCGTG CAGAGAGGCG ACCAGAGCAC CCCCAGCCCC AAGCACAGAT 
CGCTCCCTCC CTCTTTCCTC AGAGAAAGAC TTCGTGGTTC GGCAGAGAAG GGGCAAGGAG 
ACCTTAAGGA GCAGTCCTCA CAAAAAGGCC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103169.1
CDS	106..7401
Translation	MDESSILRRRGLQKELSLPRRGSLVDSQKWNCLVKRCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_030247309.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247309.1

ORF Insert Sequence:

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGA AGGAGCTGAG CCTCCCCCGA 
AGAGGAAGTC TGGTAGATTC CCAGAAGTGG AATTGCTTGG TCAAACGTTG CCGAACAAGC 
AACCGGAAGA GTTTGATAGG CAATGGCCAG TCTCCAGCAC TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GACAGTCCAA GGAATTTCTC CCCCAGTGCC 
TCAGCCCATT TCTCATTTGC GAGGAGAACG GATGGGCGCC GCTGGTCTCT GGCTTCTCTC 
CCTTCCTCAG GCTATGGGAC AAATACCCCA AGCTCCACCG TCTCCTCATC CTGTTCTTCC 
CAGGAGAAGT TGCACCAGCT ACCATACCAG CCAACCCCAG ATGAATTACA CTTCTTATCC 
AAACACTTCT GCACAACAGA AAGTATCGCC ACTGAGAACC GGTGCAGAAA CACACCCATG 
CGTCCACGTT CCCGGAGTCT CAGCCCTGGA CGGTCCCCCG CCTGCTGTGA CCATGAAATA 
ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CCACAGCTCA GATGGAAGAG 
CGTCTGAAGG AGATCATCAC CAGCTACTCT CCAGACCATG TTCTCCCCTT GGCAGATGGG 
GTACTTAGTT TCACTCACCA TCAGATCATT GAGCTGGCTC GGGACTGTTT GGATAAATCT 
CACCAGGGTC TCATCACGTC GAGATACTTT TTTGAGTTGC AGCACAAACT GGACAAGTTG 
CTCCAGGAGG CTCACGATCG CTCTGAAAGT GGAGAACTGG CATTTATCAA GCAACTAGTC 
CGAAAGATCC TAATTGTCAT TGCCCGCCCC GCTCGGTTAT TGGAGTGTTT GGAATTTGAT 
CCTGAAGAAT TTTATTACCT ATTGGAAGCT GCAGAAGGCC ATGCCAAAGA AGGCCAAGGA 
ATCAAAACTG ACATCCCTAG GTATATCATC AGCCAGCTGG GACTCAATAA GGACCCTTTA 
GAAGAAATGG CTCAGTTGGG GAATTACGAC AGTAGGACAG CAGAGACACC AGAGATGGAT 
GAGTCAGTGA GTAGCTCAAA TACTTCCCTG AGACTTCGAA GGAAACCCCG AGAGAGTGAT 
TTTGAAACAA TTAAATTGAT CAGCAATGGA GCCTACGGGG CAGTCTACTT TGTTCGGCAC 
AAAGAGTCCC GCCAGAGGTT TGCCATGAAG AAGATCAACA AGCAGAACCT CATCCTTCGG 
AACCAGATCC AGCAGGCCTT CGTGGAGCGA GACATCCTGA CTTTCGCAGA GAACCCCTTT 
GTGGTCAGCA TGTATTGCTC CTTTGAAACG AGGCGTCACT TATGCATGGT CATGGAGTAT 
GTAGAAGGGG GAGACTGTGC GACCCTAATG AAAAACATGG GACCTCTCCC AGTTGATATG 
GCCAGAATGT ATTTCGCCGA GACCGTCTTG GCCTTGGAGT ACCTGCATAA TTACGGAATC 
GTACACAGGG ACTTGAAGCC AGACAACCTG TTGGTCACCT CCATGGGGCA CATAAAACTG 
ACTGACTTCG GCTTGTCTAA GGTGGGATTA ATGAGCATGA CCACCAATCT CTATGAAGGC 
CACATAGAGA AGGACGCTCG AGAGTTCTTA GATAAACAGG TCTGTGGTAC ACCTGAGTAC 
ATTGCCCCCG AGGTGATTCT GAGACAGGGC TACGGGAAAC CCGTGGACTG GTGGGCCATG 
GGCATTATCC TCTATGAATT TCTGGTCGGA TGTGTGCCTT TCTTTGGGGA CACTCCAGAA 
GAGCTATTTG GACAAGTCAT CAGTGATGAA ATCAACTGGC CTGAAAAGGA CGAGGCCCCT 
CCTCCAGACG CTCAGGAGCT GATTACCTTG CTCCTCAGGC AGAATCCGCT GGAGAGGCTG 
GGAACAGGTG GAGCCTATGA AGTGAAGCAG CATCGTTTCT TCCGCTCCTT AGACTGGAAC 
AGTTTGCTGA GACAGAAGGC GGAATTTATT CCCCAACTGG AATCGGAGGA TGACACAAGT 
TATTTTGATA CTCGGTCAGA GAAGTATCAT CACATGGAGA CGGAGGAGGA GGACGATACA 
AACGATGAGG ACTTCACCGT GGAGATAAGG CAGTTTTCTT CCTGTTCACA CAGGTTTTCA 
AAAGTTTTCA GCAGTATAGA TCGCATAACT CAAAATTCAG GAGAAGACAA AGATGACTCT 
GAGGACAAGA CCAAAAGCAC AACGTTGCCA TCCACAGAGA CACTCAGCTG GAGTTCCGAA 
TACTCTGAAA TGCAACAGTT ATCGACCTCC AACTCTTCAG ATACTGAAAG CAACAGGTGC 
AAACTCAGCT CTGGCTTGCT CCCCAAGCTG GCTATTTCGA CAGATGGGGA ACAAGATGAG 
GCTGTCCCTT GCTCTGGAGA CCCCAGAGAG GAGCCAGAGA AACCTGTCCC TCCCTCTGAG 
GAGTGTACTC AGGAGGAGCC CGAGGTCACC ACCCCAGCCA GCACCATCAG CAGTTCCACA 
CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGGCGAAG CGAGTGTGTG 
GACAGTACAG ATAATTCCTC AAAGCCATCC AGTGAACCCA CTTCTCACGT GGCTCGACAG 
CGCTTAGAAA GCACAGAGAA AAAGAAAATT TCTGGGAAAG TCACAAAGTC CCTCTCGGCC 
AGTGCTCTGT CCCTCATGAT CCCAGGAGAT ATGTTCGCTG TATCTCCATT GGGAAGCCCA 
ATGTCCCCAC ACTCCCTGTC TTCAGACCCT TCTTCTTCAC GGGATTCCTC TCCCAGCCGA 
GACTCTTCTG CAGCATCTGC CAGTCCGCAT CAGCCCATTG TCATCCACAG CTCAGGCAAG 
AACTATGGGT TCACCATCCG TGCTATCCGC GTGTACGTGG GGGACAGTGA CATCTACACA 
GTGCACCATA TCGTCTGGAA CGTAGAAGAA GGAAGTCCCG CATACCAGGC AGGACTGAAG 
GCCGGGGATC TGATCACACA CATCAACGGA GAGCCGGTGC ACGGCCTCGT CCACACGGAA 
GTTATCGAGC TCCTGCTGAA GAGTGGGAAT AAGGTGTCTA TCACCACTAC TCCATTTGAA 
AACACATCAA TCAAAACGGG ACCAGCCAGG AGAAACAGTT ACAAGGGCCG GATGGTGAGA 
CGAAGCAAGA AGTCCAAGAA GAAGGAGAGT CTAGAAAGGA GGAGATCTCT CTTCAAGAAG 
CTGGCCAAGC AGCCTTCTCC TTTGCTCCAC ACCAGCCGAA GTTTCTCCTG CTTAAACCGG 
TCCCTGTCAT CTGGAGAGAG CCTCCCGGGT TCCCCAACTC ACAGCTTGTC CCCGAGGTCT 
CCAACACCCA GTTATCGTTC TACTCCCGAT TTTCCGTCAG GTACAAATTC CTCCCAGAGC 
AGCTCCCCAA GTTCAAGTGC CCCCAATTCT CCAGCAGGTT CAGGGCACAT CCGGCCCAGC 
ACCCTCCATG GCCTGGCTCC CAAACTCAGC GGGCAGCGAT ACCGCTCTGG AAGACGGAAG 
TCGGCTGGCA GCATCCCTCT CTCCCCGCTG GCCAGGACAC CCTCTCCCAC TCCACAGCCT 
ACCTCTCCTC AGCGTTCACC ATCCCCACTG TTGGGACACT CACTGGGCAA TGCCAAGATC 
ACTCAGGCCT TTCCTAGCAA GATGCACTCT CCCCCCACAA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CAGAGCCGCC CCGCTCCCCA CTGCTGAAAC GGGTGCAGTC GGAGGAAAAG 
TTGTCACCCT CCTATGGCAG TGACAAGAAG CTTCTGTGCT CCCGCAAGCA TAGCCTAGAG 
GTGACACAAG AGGAGGTACA GAGGGAGCAG TGTCAGCGGG AAGTGACACT GCAGAGCCTG 
GAAGAGAATG TGTGTGACGC TCCTTCCCTC AGTCGGGCCA GGCCAGTGGA GCAAGGCTGT 
CTGAAACGCC CCGTGTCCCG GAAGGTGGGC AGGCAAGAGT CTGTGGATGA CCTGGACCGG 
GACAAGCTGA AAGCCAAGGT TGTCGTAAAG AAACCAGAAG AGAAACATGA ATCGCACCAG 
AAACCTCACA GCCTTGGTGG TGATTCGGAA AGCTATGCTC TCTTCAGGCT AGAGGAGAGA 
GAGAAAAAAG TGTACTCCAA GGGGTTGGAA AGGTCAGGCC ATTTTGAAAA CACATCAGCA 
GAGTTGCCTT CTGTGGGCAG CCTGCTGAAG GACACTCTTC ACAAGCAGGC CAGCGTGAGG 
GCCAGCGAGG GGGTGACCTC AGACGGGGCA GCTTGCAGCC TGACACCAGG GGAGCACAGC 
CAGTCTCTAG GTGACTTTAA GCGGGCCTCA GCTTCTGGCA TTCTCCATGA TAGTGTGTGC 
CCCATCTCTG ATAGGCCTGC TCCTGGAAAG GTTGAATACT CGGAGAAGGC CTCTCAGGCC 
AAAGAGCTCC TTCGAAGTGA AAAACTAGAC AGCAAGCTGG CCAATATTGA TTACCTCAGA 
AAGAAAATGT CATTGGATGA CAAAGATGAC AGCCACTGTG CCATCCTGAA ACCCAAGATA 
ACATCTAGCG CCCATGAATG TCTGCCAGGG AACCCCATAC GGCCCATGGC AGGGCAACAA 
GAGACCCCGC CAGCCTCTGA AAACCGGGCA TTCATCAACA GTACCCACAC ACCTCAGATG 
AGTGCAGTTT CCTTTGTTCC TCTCAAAGCC TTAGCTGGCC GGGTAGAGAA CGGAGGGGAG 
AAAGCAGGCT TAGCTGCTCC CGAGTCCCCT GTCAGGAAGA GCCCCTCCGA GTATAAGCTA 
GAGGGCAGGT CAGTTTCATG TCTCAAGCCG ATCGAGGGCA CACTGGACAT TGCTCTCCTG 
TCTGGACCTC ACGCCTCCAA AACAGAGTTG CTTTCCCCAG AGCCTGCACA GAGTCCCAGC 
CCAGGCATCA ACGTGGGACC ATGTGTGCCA CTAGCTCTTC CTGGGAGCAG TGGGAAAAAG 
GGAGACTCCA CCAGCCTGAG AGAGCCTTCC TCAGCCAACT TAAAAGTAAA TAAATCTTAT 
CTGCTGGAGC CTCGGTTCCT ACCCCCGAGC CGGGCTCTCC AGGACTCTCT CGCAGCCTCT 
GGGCCAGAAC CGAAGTCAAA GCCGGAAAGG AAGCTCATTC ATCCTTCTGC CAGGAGCCCA 
GCAACTGTCA CAGAGAGCAA TCTTCAGCAG AAAGAGGGTG GTCCCGCCAC ACACCAAGAC 
CGCTCCACTG ACACCAGGAA CCTCCCTGGC CCAGGGCAGA CCCTACACAA TGTGGACCTA 
CCCAGGCTGT GTACACGTGC CCCACTCCCA CCGGAAGGGA CGCCCGCAAA GGAGAAGCCA 
TGTCTGAAGG AACCCTCTGC CAAGGTGAAA AGCGAGTGGT CTGCCGTGAG GGATGACGGA 
CACAGAGATC CCTGTGCGAA GCTGTGCCCG GCAGAGACTG GTAAAGCCAG CGACAGTTCC 
AAACCCCTGC CTTCCGGGGG GAGGACCCAA CCCGATTTCT ACAAGCAGAC CCAGACTTCG 
GAGAAAGCAT GGGCGCATGC AAAAACAAAC CACAAAGATA GCCAAGATGA GGTGAAGTCC 
CTGGCCAGGG AGGACTCAGC TTCACTTTTA TATGAAAAGG AGATAGGCCG GGCACGAAAA 
GGTCCTGAAC CCAAACCGGA AGTTCCTGCT ACCCGGTGCC CTCCTCAGCC ACCAGGAATT 
GAGGGTGAGA AGCGAGAAAA GCTCTCCGCT GCCCCCTCTT TGCAGAAACA GGCTCCCAAA 
GAGCCAGACA GGAAGGAACA GACTTCGCAA AGGCCTGGAG GTAGTGGCCC TCAACAACCC 
CCACCCACCA AAGAGCTGTC TAACTCAGCA TCCTGGCAGC ACGGCAGTTC TCCGAGTCAC 
ACTTTAAAGA AGGAGCCCGG GACCAAAGCT GCCGCTGCAG AACCAAGCAC CAGCCTTCAT 
GACACTCCCC GATCTGCTAC AGCCACCACC ACTGCCATTG CCACCACCAC CACTACCACC 
AGTGCCGGGC ACAGTGACTG CAGTAGCCAT AAGGCCCGGC CTGGCCCTGA CCCCAGCCCT 
TCAAAGTCTA AGCACCAAGA CAGGTCCCTC TCCTCACAGA AGCTGAGTGC TGGCTCTGCA 
AAAGGCAAAG AGCCTGTCAC TCAACCCCTG GGTGGTTCCA TCAGAGAAGG CAAGGGTGGC 
AGCAAGGGTC CAGTGGACAC ATTTTCTGCT GTCCTGACCA CCCAGGGCAA AGCAAGTGAT 
GTGCTTGTGC AGGGAGAAGG TCGGGTCTCA ATCATTGTCC ACACTGAAGA GTGTCCTCTC 
GATGCCAAAC TGAAAAACAC CAATGGAGGG TGTCCCCCAG AGATGCAGGC GAAGCATCCA 
CCCAGACAAG GACATCTCAG TGAAGCAGCA GACCAGAAGC CACTCATTGC TGGTGAGAAG 
CAAAGCCCGT CTCCAAAGCA TCCCAAACCA TCCACTGTGA AAGATTACCC CAGTCTGTGC 
AGACAGACAG ACAGAAGCCC AAGCCATCAG GCTACCACTG GGGACAGGAA GGCAGAAGGA 
AAGAAATGCA CAGACGCACT TTATGTCGCA GCCCCAGAGG GCTACAAGCC AGAGGCCAGC 
CCTTCTCTCC ACCACGGCGA GACCGGACTC AGAGGCTCAG AGAGGCCACC CATGGGCATG 
GGGAAGGGCT TCTCTGAGCC CAAGGGGAAA GGGCCAGGTC CCCAGAAGTC ACTGGCTGAA 
ACAGGCAAGC CCAGCGGTAT GAAAAGGTCA CCCTCTGCCA CCGTGCAGAG CTCTCTCCGC 
TCAGCTGCCC CCCCAGAAAA GTCTCTGAGT TACTCAGCCA GCTTTCCCGA GGCCCAGCCT 
GGAGTGCGAG AGGTCCCTGC AGCCAACAGC AGCCCCTCAT CTGCCAAGGC TACAGGGGGG 
ACCTCAGAGT TCCCAGCCCC CAGCAGCAGG GACCACAGGA AGCTTCAGTC TGGAGGAGAC 
GGCCGAAGCC AAATGATAAA GAGTGACTCT CTGCCCTCCT TCCGCCTCTC CACCTCTGCT 
CTGGAGTCAC ATTTCCAGGA TCCACAGGTG CCCATCGCAT CAGGCCACCG AGGCAGGGCA 
CTGTCAGTAA CTGCTGCCAC AGGAGAACCC AAAGGGAGAG AGCTCGCCCA GCCTCCCCCA 
GTCAGGAAAC AGAATGCGTG CAGAGAGGCG ACCAGAGCAC CCCCAGCCCC AAGCACAGAT 
CGCTCCCTCC CTCTTTCCTC AGAGAAAGAC TTCGTGGTTC GGCAGAGAAG GGGCAAGGAG 
ACCTTAAGGA GCAGTCCTCA CAAAAAGGCC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_030247310.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103170.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X18, mRNA.

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CloneID	OMu86257
Clone ID Related Accession (Same CDS sequence)	XM_030247310.1
Accession Version	XM_030247310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCCC AGCGGGAAAG ACTACAGATT CCGGGGCTGA CCTTGGATTG CCGAACAAGC 
AACCGGAAGA GTTTGATAGG CAATGGCCAG TCTCCAGCAC TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GACAGTCCAA GGAATTTCTC CCCCAGTGCC 
TCAGCCCATT TCTCATTTGC GAGGAGAACG GATGGGCGCC GCTGGTCTCT GGCTTCTCTC 
CCTTCCTCAG GCTATGGGAC AAATACCCCA AGCTCCACCG TCTCCTCATC CTGTTCTTCC 
CAGGAGAAGT TGCACCAGCT ACCATACCAG CCAACCCCAG ATGAATTACA CTTCTTATCC 
AAACACTTCT GCACAACAGA AAGTATCGCC ACTGAGAACC GGTGCAGAAA CACACCCATG 
CGTCCACGTT CCCGGAGTCT CAGCCCTGGA CGGTCCCCCG CCTGCTGTGA CCATGAAATA 
ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CCACAGCTCA GATGGAAGAG 
CGTCTGAAGG AGATCATCAC CAGCTACTCT CCAGACCATG TTCTCCCCTT GGCAGATGGG 
GTACTTAGTT TCACTCACCA TCAGATCATT GAGCTGGCTC GGGACTGTTT GGATAAATCT 
CACCAGGGTC TCATCACGTC GAGATACTTT TTTGAGTTGC AGCACAAACT GGACAAGTTG 
CTCCAGGAGG CTCACGATCG CTCTGAAAGT GGAGAACTGG CATTTATCAA GCAACTAGTC 
CGAAAGATCC TAATTGTCAT TGCCCGCCCC GCTCGGTTAT TGGAGTGTTT GGAATTTGAT 
CCTGAAGAAT TTTATTACCT ATTGGAAGCT GCAGAAGGCC ATGCCAAAGA AGGCCAAGGA 
ATCAAAACTG ACATCCCTAG GTATATCATC AGCCAGCTGG GACTCAATAA GGACCCTTTA 
GAAGAAATGG CTCAGTTGGG GAATTACGAC AGTAGGACAG CAGAGACACC AGAGATGGAT 
GAGTCAGTGA GTAGCTCAAA TACTTCCCTG AGACTTCGAA GGAAACCCCG AGAGAGTGAT 
TTTGAAACAA TTAAATTGAT CAGCAATGGA GCCTACGGGG CAGTCTACTT TGTTCGGCAC 
AAAGAGTCCC GCCAGAGGTT TGCCATGAAG AAGATCAACA AGCAGAACCT CATCCTTCGG 
AACCAGATCC AGCAGGCCTT CGTGGAGCGA GACATCCTGA CTTTCGCAGA GAACCCCTTT 
GTGGTCAGCA TGTATTGCTC CTTTGAAACG AGGCGTCACT TATGCATGGT CATGGAGTAT 
GTAGAAGGGG GAGACTGTGC GACCCTAATG AAAAACATGG GACCTCTCCC AGTTGATATG 
GCCAGAATGT ATTTCGCCGA GACCGTCTTG GCCTTGGAGT ACCTGCATAA TTACGGAATC 
GTACACAGGG ACTTGAAGCC AGACAACCTG TTGGTCACCT CCATGGGGCA CATAAAACTG 
ACTGACTTCG GCTTGTCTAA GGTGGGATTA ATGAGCATGA CCACCAATCT CTATGAAGGC 
CACATAGAGA AGGACGCTCG AGAGTTCTTA GATAAACAGG TCTGTGGTAC ACCTGAGTAC 
ATTGCCCCCG AGGTGATTCT GAGACAGGGC TACGGGAAAC CCGTGGACTG GTGGGCCATG 
GGCATTATCC TCTATGAATT TCTGGTCGGA TGTGTGCCTT TCTTTGGGGA CACTCCAGAA 
GAGCTATTTG GACAAGTCAT CAGTGATGAA ATCAACTGGC CTGAAAAGGA CGAGGCCCCT 
CCTCCAGACG CTCAGGAGCT GATTACCTTG CTCCTCAGGC AGAATCCGCT GGAGAGGCTG 
GGAACAGGTG GAGCCTATGA AGTGAAGCAG CATCGTTTCT TCCGCTCCTT AGACTGGAAC 
AGTTTGCTGA GACAGAAGGC GGAATTTATT CCCCAACTGG AATCGGAGGA TGACACAAGT 
TATTTTGATA CTCGGTCAGA GAAGTATCAT CACATGGAGA CGGAGGAGGA GGACGATACA 
AACGATGAGG ACTTCACCGT GGAGATAAGG CAGTTTTCTT CCTGTTCACA CAGGTTTTCA 
AAAGTTTTCA GCAGTATAGA TCGCATAACT CAAAATTCAG GAGAAGACAA AGATGACTCT 
GAGGACAAGA CCAAAAGCAC AACGTTGCCA TCCACAGAGA CACTCAGCTG GAGTTCCGAA 
TACTCTGAAA TGCAACAGTT ATCGACCTCC AACTCTTCAG ATACTGAAAG CAACAGGTGC 
AAACTCAGCT CTGGCTTGCT CCCCAAGCTG GCTATTTCGA CAGATGGGGA ACAAGATGAG 
GCTGTCCCTT GCTCTGGAGA CCCCAGAGAG GAGCCAGAGA AACCTGTCCC TCCCTCTGAG 
GAGTGTACTC AGGAGGAGCC CGAGGTCACC ACCCCAGCCA GCACCATCAG CAGTTCCACA 
CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGGCGAAG CGAGTGTGTG 
GACAGTACAG ATAATTCCTC AAAGCCATCC AGTGAACCCA CTTCTCACGT GGCTCGACAG 
CGCTTAGAAA GCACAGAGAA AAAGAAAATT TCTGGGAAAG TCACAAAGTC CCTCTCGGCC 
AGTGCTCTGT CCCTCATGAT CCCAGGAGAT ATGTTCGCTG TATCTCCATT GGGAAGCCCA 
ATGTCCCCAC ACTCCCTGTC TTCAGACCCT TCTTCTTCAC GGGATTCCTC TCCCAGCCGA 
GACTCTTCTG CAGCATCTGC CAGTCCGCAT CAGCCCATTG TCATCCACAG CTCAGGCAAG 
AACTATGGGT TCACCATCCG TGCTATCCGC GTGTACGTGG GGGACAGTGA CATCTACACA 
GTGCACCATA TCGTCTGGAA CGTAGAAGAA GGAAGTCCCG CATACCAGGC AGGACTGAAG 
GCCGGGGATC TGATCACACA CATCAACGGA GAGCCGGTGC ACGGCCTCGT CCACACGGAA 
GTTATCGAGC TCCTGCTGAA GAGTGGGAAT AAGGTGTCTA TCACCACTAC TCCATTTGAA 
AACACATCAA TCAAAACGGG ACCAGCCAGG AGAAACAGTT ACAAGGGCCG GATGGTGAGA 
CGAAGCAAGA AGTCCAAGAA GAAGGAGAGT CTAGAAAGGA GGAGATCTCT CTTCAAGAAG 
CTGGCCAAGC AGCCTTCTCC TTTGCTCCAC ACCAGCCGAA GTTTCTCCTG CTTAAACCGG 
TCCCTGTCAT CTGGAGAGAG CCTCCCGGGT TCCCCAACTC ACAGCTTGTC CCCGAGGTCT 
CCAACACCCA GTTATCGTTC TACTCCCGAT TTTCCGTCAG GTACAAATTC CTCCCAGAGC 
AGCTCCCCAA GTTCAAGTGC CCCCAATTCT CCAGCAGGTT CAGGGCACAT CCGGCCCAGC 
ACCCTCCATG GCCTGGCTCC CAAACTCAGC GGGCAGCGAT ACCGCTCTGG AAGACGGAAG 
TCGGCTGGCA GCATCCCTCT CTCCCCGCTG GCCAGGACAC CCTCTCCCAC TCCACAGCCT 
ACCTCTCCTC AGCGTTCACC ATCCCCACTG TTGGGACACT CACTGGGCAA TGCCAAGATC 
ACTCAGGCCT TTCCTAGCAA GATGCACTCT CCCCCCACAA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CAGAGCCGCC CCGCTCCCCA CTGCTGAAAC GGGTGCAGTC GGAGGAAAAG 
TTGTCACCCT CCTATGGCAG TGACAAGAAG CTTCTGTGCT CCCGCAAGCA TAGCCTAGAG 
GTGACACAAG AGGAGGTACA GAGGGAGCAG TGTCAGCGGG AAGTGACACT GCAGAGCCTG 
GAAGAGAATG TGTGTGACGC TCCTTCCCTC AGTCGGGCCA GGCCAGTGGA GCAAGGCTGT 
CTGAAACGCC CCGTGTCCCG GAAGGTGGGC AGGCAAGAGT CTGTGGATGA CCTGGACCGG 
GACAAGCTGA AAGCCAAGGT TGTCGTAAAG AAACCAGAAG AGAAACATGA ATCGCACCAG 
AAACCTCACA GCCTTGGTGG TGATTCGGAA AGCTATGCTC TCTTCAGGCT AGAGGAGAGA 
GAGAAAAAAG TGTACTCCAA GGGGTTGGAA AGGTCAGGCC ATTTTGAAAA CACATCAGCA 
GAGTTGCCTT CTGTGGGCAG CCTGCTGAAG GACACTCTTC ACAAGCAGGC CAGCGTGAGG 
GCCAGCGAGG GGGTGACCTC AGACGGGGCA GCTTGCAGCC TGACACCAGG GGAGCACAGC 
CAGTCTCTAG GTGACTTTAA GCGGGCCTCA GCTTCTGGCA TTCTCCATGA TAGTGTGTGC 
CCCATCTCTG ATAGGCCTGC TCCTGGAAAG GTTGAATACT CGGAGAAGGC CTCTCAGGCC 
AAAGAGCTCC TTCGAAGTGA AAAACTAGAC AGCAAGCTGG CCAATATTGA TTACCTCAGA 
AAGAAAATGT CATTGGATGA CAAAGATGAC AGCCACTGTG CCATCCTGAA ACCCAAGATA 
ACATCTAGCG CCCATGAATG TCTGCCAGGG AACCCCATAC GGCCCATGGC AGGGCAACAA 
GAGACCCCGC CAGCCTCTGA AAACCGGGCA TTCATCAACA GTACCCACAC ACCTCAGATG 
AGTGCAGTTT CCTTTGTTCC TCTCAAAGCC TTAGCTGGCC GGGTAGAGAA CGGAGGGGAG 
AAAGCAGGCT TAGCTGCTCC CGAGTCCCCT GTCAGGAAGA GCCCCTCCGA GTATAAGCTA 
GAGGGCAGGT CAGTTTCATG TCTCAAGCCG ATCGAGGGCA CACTGGACAT TGCTCTCCTG 
TCTGGACCTC ACGCCTCCAA AACAGAGTTG CTTTCCCCAG AGCCTGCACA GAGTCCCAGC 
CCAGGCATCA ACGTGGGACC ATGTGTGCCA CTAGCTCTTC CTGGGAGCAG TGGGAAAAAG 
GGAGACTCCA CCAGCCTGAG AGAGCCTTCC TCAGCCAACT TAAAAGTAAA TAAATCTTAT 
CTGCTGGAGC CTCGGTTCCT ACCCCCGAGC CGGGCTCTCC AGGACTCTCT CGCAGCCTCT 
GGGCCAGAAC CGAAGTCAAA GCCGGAAAGG AAGCTCATTC ATCCTTCTGC CAGGAGCCCA 
GCAACTGTCA CAGAGAGCAA TCTTCAGCAG AAAGAGGGTG GTCCCGCCAC ACACCAAGAC 
CGCTCCACTG ACACCAGGAA CCTCCCTGGC CCAGGGCAGA CCCTACACAA TGTGGACCTA 
CCCAGGCTGT GTACACGTGC CCCACTCCCA CCGGAAGGGA CGCCCGCAAA GGAGAAGCCA 
TGTCTGAAGG AACCCTCTGC CAAGGTGAAA AGCGAGTGGT CTGCCGTGAG GGATGACGGA 
CACAGAGATC CCTGTGCGAA GCTGTGCCCG GCAGAGACTG GTAAAGCCAG CGACAGTTCC 
AAACCCCTGC CTTCCGGGGG GAGGACCCAA CCCGATTTCT ACAAGCAGAC CCAGACTTCG 
GAGAAAGCAT GGGCGCATGC AAAAACAAAC CACAAAGATA GCCAAGATGA GGTGAAGTCC 
CTGGCCAGGG AGGACTCAGC TTCACTTTTA TATGAAAAGG AGATAGGCCG GGCACGAAAA 
GGTCCTGAAC CCAAACCGGA AGTTCCTGCT ACCCGGTGCC CTCCTCAGCC ACCAGGAATT 
GAGGGTGAGA AGCGAGAAAA GCTCTCCGCT GCCCCCTCTT TGCAGAAACA GGCTCCCAAA 
GAGCCAGACA GGAAGGAACA GACTTCGCAA AGGCCTGGAG GTAGTGGCCC TCAACAACCC 
CCACCCACCA AAGAGCTGTC TAACTCAGCA TCCTGGCAGC ACGGCAGTTC TCCGAGTCAC 
ACTTTAAAGA AGGAGCCCGG GACCAAAGCT GCCGCTGCAG AACCAAGCAC CAGCCTTCAT 
GACACTCCCC GATCTGCTAC AGCCACCACC ACTGCCATTG CCACCACCAC CACTACCACC 
AGTGCCGGGC ACAGTGACTG CAGTAGCCAT AAGGCCCGGC CTGGCCCTGA CCCCAGCCCT 
TCAAAGTCTA AGCACCAAGA CAGGTCCCTC TCCTCACAGA AGCTGAGTGC TGGCTCTGCA 
AAAGGCAAAG AGCCTGTCAC TCAACCCCTG GGTGGTTCCA TCAGAGAAGG CAAGGGTGGC 
AGCAAGGGTC CAGTGGACAC ATTTTCTGCT GTCCTGACCA CCCAGGGCAA AGCAAGTGAT 
GTGCTTGTGC AGGGAGAAGG TCGGGTCTCA ATCATTGTCC ACACTGAAGA GTGTCCTCTC 
GATGCCAAAC TGAAAAACAC CAATGGAGGG TGTCCCCCAG AGATGCAGGC GAAGCATCCA 
CCCAGACAAG GACATCTCAG TGAAGCAGCA GACCAGAAGC CACTCATTGC TGGTGAGAAG 
CAAAGCCCGT CTCCAAAGCA TCCCAAACCA TCCACTGTGA AAGATTACCC CAGTCTGTGC 
AGACAGACAG ACAGAAGCCC AAGCCATCAG GCTACCACTG GGGACAGGAA GGCAGAAGGA 
AAGAAATGCA CAGACGCACT TTATGTCGCA GCCCCAGAGG GCTACAAGCC AGAGGCCAGC 
CCTTCTCTCC ACCACGGCGA GACCGGACTC AGAGGCTCAG AGAGGCCACC CATGGGCATG 
GGGAAGGGCT TCTCTGAGCC CAAGGGGAAA GGGCCAGGTC CCCAGAAGTC ACTGGCTGAA 
ACAGGCAAGC CCAGCGGTAT GAAAAGGTCA CCCTCTGCCA CCGTGCAGAG CTCTCTCCGC 
TCAGCTGCCC CCCCAGAAAA GTCTCTGAGT TACTCAGCCA GCTTTCCCGA GGCCCAGCCT 
GGAGTGCGAG AGGTCCCTGC AGCCAACAGC AGCCCCTCAT CTGCCAAGGC TACAGGGGGG 
ACCTCAGAGT TCCCAGCCCC CAGCAGCAGG GACCACAGGA AGCTTCAGTC TGGAGGAGAC 
GGCCGAAGCC AAATGATAAA GAGTGACTCT CTGCCCTCCT TCCGCCTCTC CACCTCTGCT 
CTGGAGTCAC ATTTCCAGGA TCCACAGGTG CCCATCGCAT CAGGCCACCG AGGCAGGGCA 
CTGTCAGTAA CTGCTGCCAC AGGAGAACCC AAAGGGAGAG AGCTCGCCCA GCCTCCCCCA 
GTCAGGAAAC AGAATGCGTG CAGAGAGGCG ACCAGAGCAC CCCCAGCCCC AAGCACAGAT 
CGCTCCCTCC CTCTTTCCTC AGAGAAAGAC TTCGTGGTTC GGCAGAGAAG GGGCAAGGAG 
ACCTTAAGGA GCAGTCCTCA CAAAAAGGCC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103170.1
CDS	257..7492
Translation	MKAQRERLQIPGLTLDCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_030247310.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247310.1

ORF Insert Sequence:

ATGAAAGCCC AGCGGGAAAG ACTACAGATT CCGGGGCTGA CCTTGGATTG CCGAACAAGC 
AACCGGAAGA GTTTGATAGG CAATGGCCAG TCTCCAGCAC TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GACAGTCCAA GGAATTTCTC CCCCAGTGCC 
TCAGCCCATT TCTCATTTGC GAGGAGAACG GATGGGCGCC GCTGGTCTCT GGCTTCTCTC 
CCTTCCTCAG GCTATGGGAC AAATACCCCA AGCTCCACCG TCTCCTCATC CTGTTCTTCC 
CAGGAGAAGT TGCACCAGCT ACCATACCAG CCAACCCCAG ATGAATTACA CTTCTTATCC 
AAACACTTCT GCACAACAGA AAGTATCGCC ACTGAGAACC GGTGCAGAAA CACACCCATG 
CGTCCACGTT CCCGGAGTCT CAGCCCTGGA CGGTCCCCCG CCTGCTGTGA CCATGAAATA 
ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CCACAGCTCA GATGGAAGAG 
CGTCTGAAGG AGATCATCAC CAGCTACTCT CCAGACCATG TTCTCCCCTT GGCAGATGGG 
GTACTTAGTT TCACTCACCA TCAGATCATT GAGCTGGCTC GGGACTGTTT GGATAAATCT 
CACCAGGGTC TCATCACGTC GAGATACTTT TTTGAGTTGC AGCACAAACT GGACAAGTTG 
CTCCAGGAGG CTCACGATCG CTCTGAAAGT GGAGAACTGG CATTTATCAA GCAACTAGTC 
CGAAAGATCC TAATTGTCAT TGCCCGCCCC GCTCGGTTAT TGGAGTGTTT GGAATTTGAT 
CCTGAAGAAT TTTATTACCT ATTGGAAGCT GCAGAAGGCC ATGCCAAAGA AGGCCAAGGA 
ATCAAAACTG ACATCCCTAG GTATATCATC AGCCAGCTGG GACTCAATAA GGACCCTTTA 
GAAGAAATGG CTCAGTTGGG GAATTACGAC AGTAGGACAG CAGAGACACC AGAGATGGAT 
GAGTCAGTGA GTAGCTCAAA TACTTCCCTG AGACTTCGAA GGAAACCCCG AGAGAGTGAT 
TTTGAAACAA TTAAATTGAT CAGCAATGGA GCCTACGGGG CAGTCTACTT TGTTCGGCAC 
AAAGAGTCCC GCCAGAGGTT TGCCATGAAG AAGATCAACA AGCAGAACCT CATCCTTCGG 
AACCAGATCC AGCAGGCCTT CGTGGAGCGA GACATCCTGA CTTTCGCAGA GAACCCCTTT 
GTGGTCAGCA TGTATTGCTC CTTTGAAACG AGGCGTCACT TATGCATGGT CATGGAGTAT 
GTAGAAGGGG GAGACTGTGC GACCCTAATG AAAAACATGG GACCTCTCCC AGTTGATATG 
GCCAGAATGT ATTTCGCCGA GACCGTCTTG GCCTTGGAGT ACCTGCATAA TTACGGAATC 
GTACACAGGG ACTTGAAGCC AGACAACCTG TTGGTCACCT CCATGGGGCA CATAAAACTG 
ACTGACTTCG GCTTGTCTAA GGTGGGATTA ATGAGCATGA CCACCAATCT CTATGAAGGC 
CACATAGAGA AGGACGCTCG AGAGTTCTTA GATAAACAGG TCTGTGGTAC ACCTGAGTAC 
ATTGCCCCCG AGGTGATTCT GAGACAGGGC TACGGGAAAC CCGTGGACTG GTGGGCCATG 
GGCATTATCC TCTATGAATT TCTGGTCGGA TGTGTGCCTT TCTTTGGGGA CACTCCAGAA 
GAGCTATTTG GACAAGTCAT CAGTGATGAA ATCAACTGGC CTGAAAAGGA CGAGGCCCCT 
CCTCCAGACG CTCAGGAGCT GATTACCTTG CTCCTCAGGC AGAATCCGCT GGAGAGGCTG 
GGAACAGGTG GAGCCTATGA AGTGAAGCAG CATCGTTTCT TCCGCTCCTT AGACTGGAAC 
AGTTTGCTGA GACAGAAGGC GGAATTTATT CCCCAACTGG AATCGGAGGA TGACACAAGT 
TATTTTGATA CTCGGTCAGA GAAGTATCAT CACATGGAGA CGGAGGAGGA GGACGATACA 
AACGATGAGG ACTTCACCGT GGAGATAAGG CAGTTTTCTT CCTGTTCACA CAGGTTTTCA 
AAAGTTTTCA GCAGTATAGA TCGCATAACT CAAAATTCAG GAGAAGACAA AGATGACTCT 
GAGGACAAGA CCAAAAGCAC AACGTTGCCA TCCACAGAGA CACTCAGCTG GAGTTCCGAA 
TACTCTGAAA TGCAACAGTT ATCGACCTCC AACTCTTCAG ATACTGAAAG CAACAGGTGC 
AAACTCAGCT CTGGCTTGCT CCCCAAGCTG GCTATTTCGA CAGATGGGGA ACAAGATGAG 
GCTGTCCCTT GCTCTGGAGA CCCCAGAGAG GAGCCAGAGA AACCTGTCCC TCCCTCTGAG 
GAGTGTACTC AGGAGGAGCC CGAGGTCACC ACCCCAGCCA GCACCATCAG CAGTTCCACA 
CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGGCGAAG CGAGTGTGTG 
GACAGTACAG ATAATTCCTC AAAGCCATCC AGTGAACCCA CTTCTCACGT GGCTCGACAG 
CGCTTAGAAA GCACAGAGAA AAAGAAAATT TCTGGGAAAG TCACAAAGTC CCTCTCGGCC 
AGTGCTCTGT CCCTCATGAT CCCAGGAGAT ATGTTCGCTG TATCTCCATT GGGAAGCCCA 
ATGTCCCCAC ACTCCCTGTC TTCAGACCCT TCTTCTTCAC GGGATTCCTC TCCCAGCCGA 
GACTCTTCTG CAGCATCTGC CAGTCCGCAT CAGCCCATTG TCATCCACAG CTCAGGCAAG 
AACTATGGGT TCACCATCCG TGCTATCCGC GTGTACGTGG GGGACAGTGA CATCTACACA 
GTGCACCATA TCGTCTGGAA CGTAGAAGAA GGAAGTCCCG CATACCAGGC AGGACTGAAG 
GCCGGGGATC TGATCACACA CATCAACGGA GAGCCGGTGC ACGGCCTCGT CCACACGGAA 
GTTATCGAGC TCCTGCTGAA GAGTGGGAAT AAGGTGTCTA TCACCACTAC TCCATTTGAA 
AACACATCAA TCAAAACGGG ACCAGCCAGG AGAAACAGTT ACAAGGGCCG GATGGTGAGA 
CGAAGCAAGA AGTCCAAGAA GAAGGAGAGT CTAGAAAGGA GGAGATCTCT CTTCAAGAAG 
CTGGCCAAGC AGCCTTCTCC TTTGCTCCAC ACCAGCCGAA GTTTCTCCTG CTTAAACCGG 
TCCCTGTCAT CTGGAGAGAG CCTCCCGGGT TCCCCAACTC ACAGCTTGTC CCCGAGGTCT 
CCAACACCCA GTTATCGTTC TACTCCCGAT TTTCCGTCAG GTACAAATTC CTCCCAGAGC 
AGCTCCCCAA GTTCAAGTGC CCCCAATTCT CCAGCAGGTT CAGGGCACAT CCGGCCCAGC 
ACCCTCCATG GCCTGGCTCC CAAACTCAGC GGGCAGCGAT ACCGCTCTGG AAGACGGAAG 
TCGGCTGGCA GCATCCCTCT CTCCCCGCTG GCCAGGACAC CCTCTCCCAC TCCACAGCCT 
ACCTCTCCTC AGCGTTCACC ATCCCCACTG TTGGGACACT CACTGGGCAA TGCCAAGATC 
ACTCAGGCCT TTCCTAGCAA GATGCACTCT CCCCCCACAA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CAGAGCCGCC CCGCTCCCCA CTGCTGAAAC GGGTGCAGTC GGAGGAAAAG 
TTGTCACCCT CCTATGGCAG TGACAAGAAG CTTCTGTGCT CCCGCAAGCA TAGCCTAGAG 
GTGACACAAG AGGAGGTACA GAGGGAGCAG TGTCAGCGGG AAGTGACACT GCAGAGCCTG 
GAAGAGAATG TGTGTGACGC TCCTTCCCTC AGTCGGGCCA GGCCAGTGGA GCAAGGCTGT 
CTGAAACGCC CCGTGTCCCG GAAGGTGGGC AGGCAAGAGT CTGTGGATGA CCTGGACCGG 
GACAAGCTGA AAGCCAAGGT TGTCGTAAAG AAACCAGAAG AGAAACATGA ATCGCACCAG 
AAACCTCACA GCCTTGGTGG TGATTCGGAA AGCTATGCTC TCTTCAGGCT AGAGGAGAGA 
GAGAAAAAAG TGTACTCCAA GGGGTTGGAA AGGTCAGGCC ATTTTGAAAA CACATCAGCA 
GAGTTGCCTT CTGTGGGCAG CCTGCTGAAG GACACTCTTC ACAAGCAGGC CAGCGTGAGG 
GCCAGCGAGG GGGTGACCTC AGACGGGGCA GCTTGCAGCC TGACACCAGG GGAGCACAGC 
CAGTCTCTAG GTGACTTTAA GCGGGCCTCA GCTTCTGGCA TTCTCCATGA TAGTGTGTGC 
CCCATCTCTG ATAGGCCTGC TCCTGGAAAG GTTGAATACT CGGAGAAGGC CTCTCAGGCC 
AAAGAGCTCC TTCGAAGTGA AAAACTAGAC AGCAAGCTGG CCAATATTGA TTACCTCAGA 
AAGAAAATGT CATTGGATGA CAAAGATGAC AGCCACTGTG CCATCCTGAA ACCCAAGATA 
ACATCTAGCG CCCATGAATG TCTGCCAGGG AACCCCATAC GGCCCATGGC AGGGCAACAA 
GAGACCCCGC CAGCCTCTGA AAACCGGGCA TTCATCAACA GTACCCACAC ACCTCAGATG 
AGTGCAGTTT CCTTTGTTCC TCTCAAAGCC TTAGCTGGCC GGGTAGAGAA CGGAGGGGAG 
AAAGCAGGCT TAGCTGCTCC CGAGTCCCCT GTCAGGAAGA GCCCCTCCGA GTATAAGCTA 
GAGGGCAGGT CAGTTTCATG TCTCAAGCCG ATCGAGGGCA CACTGGACAT TGCTCTCCTG 
TCTGGACCTC ACGCCTCCAA AACAGAGTTG CTTTCCCCAG AGCCTGCACA GAGTCCCAGC 
CCAGGCATCA ACGTGGGACC ATGTGTGCCA CTAGCTCTTC CTGGGAGCAG TGGGAAAAAG 
GGAGACTCCA CCAGCCTGAG AGAGCCTTCC TCAGCCAACT TAAAAGTAAA TAAATCTTAT 
CTGCTGGAGC CTCGGTTCCT ACCCCCGAGC CGGGCTCTCC AGGACTCTCT CGCAGCCTCT 
GGGCCAGAAC CGAAGTCAAA GCCGGAAAGG AAGCTCATTC ATCCTTCTGC CAGGAGCCCA 
GCAACTGTCA CAGAGAGCAA TCTTCAGCAG AAAGAGGGTG GTCCCGCCAC ACACCAAGAC 
CGCTCCACTG ACACCAGGAA CCTCCCTGGC CCAGGGCAGA CCCTACACAA TGTGGACCTA 
CCCAGGCTGT GTACACGTGC CCCACTCCCA CCGGAAGGGA CGCCCGCAAA GGAGAAGCCA 
TGTCTGAAGG AACCCTCTGC CAAGGTGAAA AGCGAGTGGT CTGCCGTGAG GGATGACGGA 
CACAGAGATC CCTGTGCGAA GCTGTGCCCG GCAGAGACTG GTAAAGCCAG CGACAGTTCC 
AAACCCCTGC CTTCCGGGGG GAGGACCCAA CCCGATTTCT ACAAGCAGAC CCAGACTTCG 
GAGAAAGCAT GGGCGCATGC AAAAACAAAC CACAAAGATA GCCAAGATGA GGTGAAGTCC 
CTGGCCAGGG AGGACTCAGC TTCACTTTTA TATGAAAAGG AGATAGGCCG GGCACGAAAA 
GGTCCTGAAC CCAAACCGGA AGTTCCTGCT ACCCGGTGCC CTCCTCAGCC ACCAGGAATT 
GAGGGTGAGA AGCGAGAAAA GCTCTCCGCT GCCCCCTCTT TGCAGAAACA GGCTCCCAAA 
GAGCCAGACA GGAAGGAACA GACTTCGCAA AGGCCTGGAG GTAGTGGCCC TCAACAACCC 
CCACCCACCA AAGAGCTGTC TAACTCAGCA TCCTGGCAGC ACGGCAGTTC TCCGAGTCAC 
ACTTTAAAGA AGGAGCCCGG GACCAAAGCT GCCGCTGCAG AACCAAGCAC CAGCCTTCAT 
GACACTCCCC GATCTGCTAC AGCCACCACC ACTGCCATTG CCACCACCAC CACTACCACC 
AGTGCCGGGC ACAGTGACTG CAGTAGCCAT AAGGCCCGGC CTGGCCCTGA CCCCAGCCCT 
TCAAAGTCTA AGCACCAAGA CAGGTCCCTC TCCTCACAGA AGCTGAGTGC TGGCTCTGCA 
AAAGGCAAAG AGCCTGTCAC TCAACCCCTG GGTGGTTCCA TCAGAGAAGG CAAGGGTGGC 
AGCAAGGGTC CAGTGGACAC ATTTTCTGCT GTCCTGACCA CCCAGGGCAA AGCAAGTGAT 
GTGCTTGTGC AGGGAGAAGG TCGGGTCTCA ATCATTGTCC ACACTGAAGA GTGTCCTCTC 
GATGCCAAAC TGAAAAACAC CAATGGAGGG TGTCCCCCAG AGATGCAGGC GAAGCATCCA 
CCCAGACAAG GACATCTCAG TGAAGCAGCA GACCAGAAGC CACTCATTGC TGGTGAGAAG 
CAAAGCCCGT CTCCAAAGCA TCCCAAACCA TCCACTGTGA AAGATTACCC CAGTCTGTGC 
AGACAGACAG ACAGAAGCCC AAGCCATCAG GCTACCACTG GGGACAGGAA GGCAGAAGGA 
AAGAAATGCA CAGACGCACT TTATGTCGCA GCCCCAGAGG GCTACAAGCC AGAGGCCAGC 
CCTTCTCTCC ACCACGGCGA GACCGGACTC AGAGGCTCAG AGAGGCCACC CATGGGCATG 
GGGAAGGGCT TCTCTGAGCC CAAGGGGAAA GGGCCAGGTC CCCAGAAGTC ACTGGCTGAA 
ACAGGCAAGC CCAGCGGTAT GAAAAGGTCA CCCTCTGCCA CCGTGCAGAG CTCTCTCCGC 
TCAGCTGCCC CCCCAGAAAA GTCTCTGAGT TACTCAGCCA GCTTTCCCGA GGCCCAGCCT 
GGAGTGCGAG AGGTCCCTGC AGCCAACAGC AGCCCCTCAT CTGCCAAGGC TACAGGGGGG 
ACCTCAGAGT TCCCAGCCCC CAGCAGCAGG GACCACAGGA AGCTTCAGTC TGGAGGAGAC 
GGCCGAAGCC AAATGATAAA GAGTGACTCT CTGCCCTCCT TCCGCCTCTC CACCTCTGCT 
CTGGAGTCAC ATTTCCAGGA TCCACAGGTG CCCATCGCAT CAGGCCACCG AGGCAGGGCA 
CTGTCAGTAA CTGCTGCCAC AGGAGAACCC AAAGGGAGAG AGCTCGCCCA GCCTCCCCCA 
GTCAGGAAAC AGAATGCGTG CAGAGAGGCG ACCAGAGCAC CCCCAGCCCC AAGCACAGAT 
CGCTCCCTCC CTCTTTCCTC AGAGAAAGAC TTCGTGGTTC GGCAGAGAAG GGGCAAGGAG 
ACCTTAAGGA GCAGTCCTCA CAAAAAGGCC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_006517701.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517764.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X7, mRNA.

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CloneID	OMu49077
Clone ID Related Accession (Same CDS sequence)	XM_006517701.3 , XM_006517701.4
Accession Version	XM_006517701.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517701.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGG AAGGGCAACC TCCGAAGAGC 
CATTTCAACT CAGCTCGACA TCGGCAGAGA CTAGTGGACC CAGCTGCTTC AAAAAAGGAG 
CTGAGCCTCC CCCGAAGAGG AAGTCTGGTA GATTCCCAGA AGTGGAATTG CTTGGTCAAA 
CGTTGCCGAA CAAGCAACCG GAAGAGTTTG ATAGGCAATG GCCAGTCTCC AGCACTGCCT 
CGACCACACT CACCTCTCTC TGCTCATGCA GGAAATAGCC CTCAAGACAG TCCAAGGAAT 
TTCTCCCCCA GTGCCTCAGC CCATTTCTCA TTTGCGAGGA GAAATGACAG AACGGATGGG 
CGCCGCTGGT CTCTGGCTTC TCTCCCTTCC TCAGGCTATG GGACAAATAC CCCAAGCTCC 
ACCGTCTCCT CATCCTGTTC TTCCCAGGAG AAGTTGCACC AGCTACCATA CCAGCCAACC 
CCAGATGAAT TACACTTCTT ATCCAAACAC TTCTGCACAA CAGAAAGTAT CGCCACTGAG 
AACCGGTGCA GAAACACACC CATGCGTCCA CGTTCCCGGA GTCTCAGCCC TGGACGGTCC 
CCCGCCTGCT GTGACCATGA AATAATTATG ATGAACCATG TCTACAAAGA AAGGTTCCCA 
AAGGCCACAG CTCAGATGGA AGAGCGTCTG AAGGAGATCA TCACCAGCTA CTCTCCAGAC 
CATGTTCTCC CCTTGGCAGA TGGGGTACTT AGTTTCACTC ACCATCAGAT CATTGAGCTG 
GCTCGGGACT GTTTGGATAA ATCTCACCAG GGTCTCATCA CGTCGAGATA CTTTTTTGAG 
TTGCAGCACA AACTGGACAA GTTGCTCCAG GAGGCTCACG ATCGCTCTGA AAGTGGAGAA 
CTGGCATTTA TCAAGCAACT AGTCCGAAAG ATCCTAATTG TCATTGCCCG CCCCGCTCGG 
TTATTGGAGT GTTTGGAATT TGATCCTGAA GAATTTTATT ACCTATTGGA AGCTGCAGAA 
GGCCATGCCA AAGAAGGCCA AGGAATCAAA ACTGACATCC CTAGGTATAT CATCAGCCAG 
CTGGGACTCA ATAAGGACCC TTTAGAAGAA ATGGCTCAGT TGGGGAATTA CGACAGTAGG 
ACAGCAGAGA CACCAGAGAT GGATGAGTCA GTGAGTAGCT CAAATACTTC CCTGAGACTT 
CGAAGGAAAC CCCGAGAGAG TGATTTTGAA ACAATTAAAT TGATCAGCAA TGGAGCCTAC 
GGGGCAGTCT ACTTTGTTCG GCACAAAGAG TCCCGCCAGA GGTTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTCATCCT TCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGAGACATC 
CTGACTTTCG CAGAGAACCC CTTTGTGGTC AGCATGTATT GCTCCTTTGA AACGAGGCGT 
CACTTATGCA TGGTCATGGA GTATGTAGAA GGGGGAGACT GTGCGACCCT AATGAAAAAC 
ATGGGACCTC TCCCAGTTGA TATGGCCAGA ATGTATTTCG CCGAGACCGT CTTGGCCTTG 
GAGTACCTGC ATAATTACGG AATCGTACAC AGGGACTTGA AGCCAGACAA CCTGTTGGTC 
ACCTCCATGG GGCACATAAA ACTGACTGAC TTCGGCTTGT CTAAGGTGGG ATTAATGAGC 
ATGACCACCA ATCTCTATGA AGGCCACATA GAGAAGGACG CTCGAGAGTT CTTAGATAAA 
CAGGTCTGTG GTACACCTGA GTACATTGCC CCCGAGGTGA TTCTGAGACA GGGCTACGGG 
AAACCCGTGG ACTGGTGGGC CATGGGCATT ATCCTCTATG AATTTCTGGT CGGATGTGTG 
CCTTTCTTTG GGGACACTCC AGAAGAGCTA TTTGGACAAG TCATCAGTGA TGAAATCAAC 
TGGCCTGAAA AGGACGAGGC CCCTCCTCCA GACGCTCAGG AGCTGATTAC CTTGCTCCTC 
AGGCAGAATC CGCTGGAGAG GCTGGGAACA GGTGGAGCCT ATGAAGTGAA GCAGCATCGT 
TTCTTCCGCT CCTTAGACTG GAACAGTTTG CTGAGACAGA AGGCGGAATT TATTCCCCAA 
CTGGAATCGG AGGATGACAC AAGTTATTTT GATACTCGGT CAGAGAAGTA TCATCACATG 
GAGACGGAGG AGGAGGACGA TACAAACGAT GAGGACTTCA CCGTGGAGAT AAGGCAGTTT 
TCTTCCTGTT CACACAGGTT TTCAAAAGTT TTCAGCAGTA TAGATCGCAT AACTCAAAAT 
TCAGGAGAAG ACAAAGATGA CTCTGAGGAC AAGACCAAAA GCACAACGTT GCCATCCACA 
GAGACACTCA GCTGGAGTTC CGAATACTCT GAAATGCAAC AGTTATCGAC CTCCAACTCT 
TCAGATACTG AAAGCAACAG GTGCAAACTC AGCTCTGGCT TGCTCCCCAA GCTGGCTATT 
TCGACAGATG GGGAACAAGA TGAGGCTGTC CCTTGCTCTG GAGACCCCAG AGAGGAGCCA 
GAGAAACCTG TCCCTCCCTC TGAGGAGTGT ACTCAGGAGG AGCCCGAGGT CACCACCCCA 
GCCAGCACCA TCAGCAGTTC CACACTGTCA GTTGGCAGTT TTTCAGAGCA CTTGGATCAG 
ATAAATGGGC GAAGCGAGTG TGTGGACAGT ACAGATAATT CCTCAAAGCC ATCCAGTGAA 
CCCACTTCTC ACGTGGCTCG ACAGCGCTTA GAAAGCACAG AGAAAAAGAA AATTTCTGGG 
AAAGTCACAA AGTCCCTCTC GGCCAGTGCT CTGTCCCTCA TGATCCCAGG AGATATGTTC 
GCTGTATCTC CATTGGGAAG CCCAATGTCC CCACACTCCC TGTCTTCAGA CCCTTCTTCT 
TCACGGGATT CCTCTCCCAG CCGAGACTCT TCTGCAGCAT CTGCCAGTCC GCATCAGCCC 
ATTGTCATCC ACAGCTCAGG CAAGAACTAT GGGTTCACCA TCCGTGCTAT CCGCGTGTAC 
GTGGGGGACA GTGACATCTA CACAGTGCAC CATATCGTCT GGAACGTAGA AGAAGGAAGT 
CCCGCATACC AGGCAGGACT GAAGGCCGGG GATCTGATCA CACACATCAA CGGAGAGCCG 
GTGCACGGCC TCGTCCACAC GGAAGTTATC GAGCTCCTGC TGAAGAGTGG GAATAAGGTG 
TCTATCACCA CTACTCCATT TGAAAACACA TCAATCAAAA CGGGACCAGC CAGGAGAAAC 
AGTTACAAGG GCCGGATGGT GAGACGAAGC AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA 
AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC AAGCAGCCTT CTCCTTTGCT CCACACCAGC 
CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG TCATCTGGAG AGAGCCTCCC GGGTTCCCCA 
ACTCACAGCT TGTCCCCGAG GTCTCCAACA CCCAGTTATC GTTCTACTCC CGATTTTCCG 
TCAGGTACAA ATTCCTCCCA GAGCAGCTCC CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA 
GGTTCAGGGC ACATCCGGCC CAGCACCCTC CATGGCCTGG CTCCCAAACT CAGCGGGCAG 
CGATACCGCT CTGGAAGACG GAAGTCGGCT GGCAGCATCC CTCTCTCCCC GCTGGCCAGG 
ACACCCTCTC CCACTCCACA GCCTACCTCT CCTCAGCGTT CACCATCCCC ACTGTTGGGA 
CACTCACTGG GCAATGCCAA GATCACTCAG GCCTTTCCTA GCAAGATGCA CTCTCCCCCC 
ACAATCGTCA GACACATCGT GAGGCCCAAG AGTGCAGAGC CGCCCCGCTC CCCACTGCTG 
AAACGGGTGC AGTCGGAGGA AAAGTTGTCA CCCTCCTATG GCAGTGACAA GAAGCTTCTG 
TGCTCCCGCA AGCATAGCCT AGAGGTGACA CAAGAGGAGG TACAGAGGGA GCAGTGTCAG 
CGGGAAGTGA CACTGCAGAG CCTGGAAGAG AATGTGTGTG ACGCTCCTTC CCTCAGTCGG 
GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA 
GAGTCTGTGG ATGACCTGGA CCGGGACAAG CTGAAAGCCA AGGTTGTCGT AAAGAAACCA 
GAAGAGAAAC ATGAATCGCA CCAGAAACCT CACAGCCTTG GTGGTGATTC GGAAAGCTAT 
GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA AAAGTGTACT CCAAGGGGTT GGAAAGGTCA 
GGCCATTTTG AAAACACATC AGCAGAGTTG CCTTCTGTGG GCAGCCTGCT GAAGGACACT 
CTTCACAAGC AGGCCAGCGT GAGGGCCAGC GAGGGGGTGA CCTCAGACGG GGCAGCTTGC 
AGCCTGACAC CAGGGGAGCA CAGCCAGTCT CTAGGTGACT TTAAGCGGGC CTCAGCTTCT 
GGCATTCTCC ATGATAGTGT GTGCCCCATC TCTGATAGGC CTGCTCCTGG AAAGGTTGAA 
TACTCGGAGA AGGCCTCTCA GGCCAAAGAG CTCCTTCGAA GTGAAAAACT AGACAGCAAG 
CTGGCCAATA TTGATTACCT CAGAAAGAAA ATGTCATTGG ATGACAAAGA TGACAGCCAC 
TGTGCCATCC TGAAACCCAA GATAACATCT AGCGCCCATG AATGTCTGCC AGGGAACCCC 
ATACGGCCCA TGGCAGGGCA ACAAGAGACC CCGCCAGCCT CTGAAAACCG GGCATTCATC 
AACAGTACCC ACACACCTCA GATGAGTGCA GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT 
GGCCGGGTAG AGAACGGAGG GGAGAAAGCA GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG 
AAGAGCCCCT CCGAGTATAA GCTAGAGGGC AGGTCAGTTT CATGTCTCAA GCCGATCGAG 
GGCACACTGG ACATTGCTCT CCTGTCTGGA CCTCACGCCT CCAAAACAGA GTTGCTTTCC 
CCAGAGCCTG CACAGAGTCC CAGCCCAGGC ATCAACGTGG GACCATGTGT GCCACTAGCT 
CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC TCCACCAGCC TGAGAGAGCC TTCCTCAGCC 
AACTTAAAAG TAAATAAATC TTATCTGCTG GAGCCTCGGT TCCTACCCCC GAGCCGGGCT 
CTCCAGGACT CTCTCGCAGC CTCTGGGCCA GAACCGAAGT CAAAGCCGGA AAGGAAGCTC 
ATTCATCCTT CTGCCAGGAG CCCAGCAACT GTCACAGAGA GCAATCTTCA GCAGAAAGAG 
GGTGGTCCCG CCACACACCA AGACCGCTCC ACTGACACCA GGAACCTCCC TGGCCCAGGG 
CAGACCCTAC ACAATGTGGA CCTACCCAGG CTGTGTACAC GTGCCCCACT CCCACCGGAA 
GGGACGCCCG CAAAGGAGAA GCCATGTCTG AAGGAACCCT CTGCCAAGGT GAAAAGCGAG 
TGGTCTGCCG TGAGGGATGA CGGACACAGA GATCCCTGTG CGAAGCTGTG CCCGGCAGAG 
ACTGGTAAAG CCAGCGACAG TTCCAAACCC CTGCCTTCCG GGGGGAGGAC CCAACCCGAT 
TTCTACAAGC AGACCCAGAC TTCGGAGAAA GCATGGGCGC ATGCAAAAAC AAACCACAAA 
GATAGCCAAG ATGAGGTGAA GTCCCTGGCC AGGGAGGACT CAGCTTCACT TTTATATGAA 
AAGGAGATAG GCCGGGCACG AAAAGGTCCT GAACCCAAAC CGGAAGTTCC TGCTACCCGG 
TGCCCTCCTC AGCCACCAGG AATTGAGGGT GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC 
TCTTTGCAGA AACAGGCTCC CAAAGAGCCA GACAGGAAGG AACAGACTTC GCAAAGGCCT 
GGAGGTAGTG GCCCTCAACA ACCCCCACCC ACCAAAGAGC TGTCTAACTC AGCATCCTGG 
CAGCACGGCA GTTCTCCGAG TCACACTTTA AAGAAGGAGC CCGGGACCAA AGCTGCCGCT 
GCAGAACCAA GCACCAGCCT TCATGACACT CCCCGATCTG CTACAGCCAC CACCACTGCC 
ATTGCCACCA CCACCACTAC CACCAGTGCC GGGCACAGTG ACTGCAGTAG CCATAAGGCC 
CGGCCTGGCC CTGACCCCAG CCCTTCAAAG TCTAAGCACC AAGACAGGTC CCTCTCCTCA 
CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC AAAGAGCCTG TCACTCAACC CCTGGGTGGT 
TCCATCAGAG AAGGCAAGGG TGGCAGCAAG GGTCCAGTGG ACACATTTTC TGCTGTCCTG 
ACCACCCAGG GCAAAGCAAG TGATGTGCTT GTGCAGGGAG AAGGTCGGGT CTCAATCATT 
GTCCACACTG AAGAGTGTCC TCTCGATGCC AAACTGAAAA ACACCAATGG AGGGTGTCCC 
CCAGAGATGC AGGCGAAGCA TCCACCCAGA CAAGGACATC TCAGTGAAGC AGCAGACCAG 
AAGCCACTCA TTGCTGGTGA GAAGCAAAGC CCGTCTCCAA AGCATCCCAA ACCATCCACT 
GTGAAAGATT ACCCCAGTCT GTGCAGACAG ACAGACAGAA GCCCAAGCCA TCAGGCTACC 
ACTGGGGACA GGAAGGCAGA AGGAAAGAAA TGCACAGACG CACTTTATGT CGCAGCCCCA 
GAGGGCTACA AGCCAGAGGC CAGCCCTTCT CTCCACCACG GCGAGACCGG ACTCAGAGGC 
TCAGAGAGGC CACCCATGGG CATGGGGAAG GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA 
GGTCCCCAGA AGTCACTGGC TGAAACAGGC AAGCCCAGCG GTATGAAAAG GTCACCCTCT 
GCCACCGTGC AGAGCTCTCT CCGCTCAGCT GCCCCCCCAG AAAAGTCTCT GAGTTACTCA 
GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC 
TCATCTGCCA AGGCTACAGG GGGGACCTCA GAGTTCCCAG CCCCCAGCAG CAGGGACCAC 
AGGAAGCTTC AGTCTGGAGG AGACGGCCGA AGCCAAATGA TAAAGAGTGA CTCTCTGCCC 
TCCTTCCGCC TCTCCACCTC TGCTCTGGAG TCACATTTCC AGGATCCACA GGTGCCCATC 
GCATCAGGCC ACCGAGGCAG GGCACTGTCA GTAACTGCTG CCACAGGAGA ACCCAAAGGG 
AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG AAACAGAATG CGTGCAGAGA GGCGACCAGA 
GCACCCCCAG CCCCAAGCAC AGATCGCTCC CTCCCTCTTT CCTCAGAGAA AGACTTCGTG 
GTTCGGCAGA GAAGGGGCAA GGAGACCTTA AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517764.1
CDS	777..8156
Translation	MDESSILRRRGLQEGQPPKSHFNSARHRQRLVDPAASKKELSLPRRGSLVDSQKWNCLVKRCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_006517701.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517701.3

ORF Insert Sequence:

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGG AAGGGCAACC TCCGAAGAGC 
CATTTCAACT CAGCTCGACA TCGGCAGAGA CTAGTGGACC CAGCTGCTTC AAAAAAGGAG 
CTGAGCCTCC CCCGAAGAGG AAGTCTGGTA GATTCCCAGA AGTGGAATTG CTTGGTCAAA 
CGTTGCCGAA CAAGCAACCG GAAGAGTTTG ATAGGCAATG GCCAGTCTCC AGCACTGCCT 
CGACCACACT CACCTCTCTC TGCTCATGCA GGAAATAGCC CTCAAGACAG TCCAAGGAAT 
TTCTCCCCCA GTGCCTCAGC CCATTTCTCA TTTGCGAGGA GAAATGACAG AACGGATGGG 
CGCCGCTGGT CTCTGGCTTC TCTCCCTTCC TCAGGCTATG GGACAAATAC CCCAAGCTCC 
ACCGTCTCCT CATCCTGTTC TTCCCAGGAG AAGTTGCACC AGCTACCATA CCAGCCAACC 
CCAGATGAAT TACACTTCTT ATCCAAACAC TTCTGCACAA CAGAAAGTAT CGCCACTGAG 
AACCGGTGCA GAAACACACC CATGCGTCCA CGTTCCCGGA GTCTCAGCCC TGGACGGTCC 
CCCGCCTGCT GTGACCATGA AATAATTATG ATGAACCATG TCTACAAAGA AAGGTTCCCA 
AAGGCCACAG CTCAGATGGA AGAGCGTCTG AAGGAGATCA TCACCAGCTA CTCTCCAGAC 
CATGTTCTCC CCTTGGCAGA TGGGGTACTT AGTTTCACTC ACCATCAGAT CATTGAGCTG 
GCTCGGGACT GTTTGGATAA ATCTCACCAG GGTCTCATCA CGTCGAGATA CTTTTTTGAG 
TTGCAGCACA AACTGGACAA GTTGCTCCAG GAGGCTCACG ATCGCTCTGA AAGTGGAGAA 
CTGGCATTTA TCAAGCAACT AGTCCGAAAG ATCCTAATTG TCATTGCCCG CCCCGCTCGG 
TTATTGGAGT GTTTGGAATT TGATCCTGAA GAATTTTATT ACCTATTGGA AGCTGCAGAA 
GGCCATGCCA AAGAAGGCCA AGGAATCAAA ACTGACATCC CTAGGTATAT CATCAGCCAG 
CTGGGACTCA ATAAGGACCC TTTAGAAGAA ATGGCTCAGT TGGGGAATTA CGACAGTAGG 
ACAGCAGAGA CACCAGAGAT GGATGAGTCA GTGAGTAGCT CAAATACTTC CCTGAGACTT 
CGAAGGAAAC CCCGAGAGAG TGATTTTGAA ACAATTAAAT TGATCAGCAA TGGAGCCTAC 
GGGGCAGTCT ACTTTGTTCG GCACAAAGAG TCCCGCCAGA GGTTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTCATCCT TCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGAGACATC 
CTGACTTTCG CAGAGAACCC CTTTGTGGTC AGCATGTATT GCTCCTTTGA AACGAGGCGT 
CACTTATGCA TGGTCATGGA GTATGTAGAA GGGGGAGACT GTGCGACCCT AATGAAAAAC 
ATGGGACCTC TCCCAGTTGA TATGGCCAGA ATGTATTTCG CCGAGACCGT CTTGGCCTTG 
GAGTACCTGC ATAATTACGG AATCGTACAC AGGGACTTGA AGCCAGACAA CCTGTTGGTC 
ACCTCCATGG GGCACATAAA ACTGACTGAC TTCGGCTTGT CTAAGGTGGG ATTAATGAGC 
ATGACCACCA ATCTCTATGA AGGCCACATA GAGAAGGACG CTCGAGAGTT CTTAGATAAA 
CAGGTCTGTG GTACACCTGA GTACATTGCC CCCGAGGTGA TTCTGAGACA GGGCTACGGG 
AAACCCGTGG ACTGGTGGGC CATGGGCATT ATCCTCTATG AATTTCTGGT CGGATGTGTG 
CCTTTCTTTG GGGACACTCC AGAAGAGCTA TTTGGACAAG TCATCAGTGA TGAAATCAAC 
TGGCCTGAAA AGGACGAGGC CCCTCCTCCA GACGCTCAGG AGCTGATTAC CTTGCTCCTC 
AGGCAGAATC CGCTGGAGAG GCTGGGAACA GGTGGAGCCT ATGAAGTGAA GCAGCATCGT 
TTCTTCCGCT CCTTAGACTG GAACAGTTTG CTGAGACAGA AGGCGGAATT TATTCCCCAA 
CTGGAATCGG AGGATGACAC AAGTTATTTT GATACTCGGT CAGAGAAGTA TCATCACATG 
GAGACGGAGG AGGAGGACGA TACAAACGAT GAGGACTTCA CCGTGGAGAT AAGGCAGTTT 
TCTTCCTGTT CACACAGGTT TTCAAAAGTT TTCAGCAGTA TAGATCGCAT AACTCAAAAT 
TCAGGAGAAG ACAAAGATGA CTCTGAGGAC AAGACCAAAA GCACAACGTT GCCATCCACA 
GAGACACTCA GCTGGAGTTC CGAATACTCT GAAATGCAAC AGTTATCGAC CTCCAACTCT 
TCAGATACTG AAAGCAACAG GTGCAAACTC AGCTCTGGCT TGCTCCCCAA GCTGGCTATT 
TCGACAGATG GGGAACAAGA TGAGGCTGTC CCTTGCTCTG GAGACCCCAG AGAGGAGCCA 
GAGAAACCTG TCCCTCCCTC TGAGGAGTGT ACTCAGGAGG AGCCCGAGGT CACCACCCCA 
GCCAGCACCA TCAGCAGTTC CACACTGTCA GTTGGCAGTT TTTCAGAGCA CTTGGATCAG 
ATAAATGGGC GAAGCGAGTG TGTGGACAGT ACAGATAATT CCTCAAAGCC ATCCAGTGAA 
CCCACTTCTC ACGTGGCTCG ACAGCGCTTA GAAAGCACAG AGAAAAAGAA AATTTCTGGG 
AAAGTCACAA AGTCCCTCTC GGCCAGTGCT CTGTCCCTCA TGATCCCAGG AGATATGTTC 
GCTGTATCTC CATTGGGAAG CCCAATGTCC CCACACTCCC TGTCTTCAGA CCCTTCTTCT 
TCACGGGATT CCTCTCCCAG CCGAGACTCT TCTGCAGCAT CTGCCAGTCC GCATCAGCCC 
ATTGTCATCC ACAGCTCAGG CAAGAACTAT GGGTTCACCA TCCGTGCTAT CCGCGTGTAC 
GTGGGGGACA GTGACATCTA CACAGTGCAC CATATCGTCT GGAACGTAGA AGAAGGAAGT 
CCCGCATACC AGGCAGGACT GAAGGCCGGG GATCTGATCA CACACATCAA CGGAGAGCCG 
GTGCACGGCC TCGTCCACAC GGAAGTTATC GAGCTCCTGC TGAAGAGTGG GAATAAGGTG 
TCTATCACCA CTACTCCATT TGAAAACACA TCAATCAAAA CGGGACCAGC CAGGAGAAAC 
AGTTACAAGG GCCGGATGGT GAGACGAAGC AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA 
AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC AAGCAGCCTT CTCCTTTGCT CCACACCAGC 
CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG TCATCTGGAG AGAGCCTCCC GGGTTCCCCA 
ACTCACAGCT TGTCCCCGAG GTCTCCAACA CCCAGTTATC GTTCTACTCC CGATTTTCCG 
TCAGGTACAA ATTCCTCCCA GAGCAGCTCC CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA 
GGTTCAGGGC ACATCCGGCC CAGCACCCTC CATGGCCTGG CTCCCAAACT CAGCGGGCAG 
CGATACCGCT CTGGAAGACG GAAGTCGGCT GGCAGCATCC CTCTCTCCCC GCTGGCCAGG 
ACACCCTCTC CCACTCCACA GCCTACCTCT CCTCAGCGTT CACCATCCCC ACTGTTGGGA 
CACTCACTGG GCAATGCCAA GATCACTCAG GCCTTTCCTA GCAAGATGCA CTCTCCCCCC 
ACAATCGTCA GACACATCGT GAGGCCCAAG AGTGCAGAGC CGCCCCGCTC CCCACTGCTG 
AAACGGGTGC AGTCGGAGGA AAAGTTGTCA CCCTCCTATG GCAGTGACAA GAAGCTTCTG 
TGCTCCCGCA AGCATAGCCT AGAGGTGACA CAAGAGGAGG TACAGAGGGA GCAGTGTCAG 
CGGGAAGTGA CACTGCAGAG CCTGGAAGAG AATGTGTGTG ACGCTCCTTC CCTCAGTCGG 
GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA 
GAGTCTGTGG ATGACCTGGA CCGGGACAAG CTGAAAGCCA AGGTTGTCGT AAAGAAACCA 
GAAGAGAAAC ATGAATCGCA CCAGAAACCT CACAGCCTTG GTGGTGATTC GGAAAGCTAT 
GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA AAAGTGTACT CCAAGGGGTT GGAAAGGTCA 
GGCCATTTTG AAAACACATC AGCAGAGTTG CCTTCTGTGG GCAGCCTGCT GAAGGACACT 
CTTCACAAGC AGGCCAGCGT GAGGGCCAGC GAGGGGGTGA CCTCAGACGG GGCAGCTTGC 
AGCCTGACAC CAGGGGAGCA CAGCCAGTCT CTAGGTGACT TTAAGCGGGC CTCAGCTTCT 
GGCATTCTCC ATGATAGTGT GTGCCCCATC TCTGATAGGC CTGCTCCTGG AAAGGTTGAA 
TACTCGGAGA AGGCCTCTCA GGCCAAAGAG CTCCTTCGAA GTGAAAAACT AGACAGCAAG 
CTGGCCAATA TTGATTACCT CAGAAAGAAA ATGTCATTGG ATGACAAAGA TGACAGCCAC 
TGTGCCATCC TGAAACCCAA GATAACATCT AGCGCCCATG AATGTCTGCC AGGGAACCCC 
ATACGGCCCA TGGCAGGGCA ACAAGAGACC CCGCCAGCCT CTGAAAACCG GGCATTCATC 
AACAGTACCC ACACACCTCA GATGAGTGCA GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT 
GGCCGGGTAG AGAACGGAGG GGAGAAAGCA GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG 
AAGAGCCCCT CCGAGTATAA GCTAGAGGGC AGGTCAGTTT CATGTCTCAA GCCGATCGAG 
GGCACACTGG ACATTGCTCT CCTGTCTGGA CCTCACGCCT CCAAAACAGA GTTGCTTTCC 
CCAGAGCCTG CACAGAGTCC CAGCCCAGGC ATCAACGTGG GACCATGTGT GCCACTAGCT 
CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC TCCACCAGCC TGAGAGAGCC TTCCTCAGCC 
AACTTAAAAG TAAATAAATC TTATCTGCTG GAGCCTCGGT TCCTACCCCC GAGCCGGGCT 
CTCCAGGACT CTCTCGCAGC CTCTGGGCCA GAACCGAAGT CAAAGCCGGA AAGGAAGCTC 
ATTCATCCTT CTGCCAGGAG CCCAGCAACT GTCACAGAGA GCAATCTTCA GCAGAAAGAG 
GGTGGTCCCG CCACACACCA AGACCGCTCC ACTGACACCA GGAACCTCCC TGGCCCAGGG 
CAGACCCTAC ACAATGTGGA CCTACCCAGG CTGTGTACAC GTGCCCCACT CCCACCGGAA 
GGGACGCCCG CAAAGGAGAA GCCATGTCTG AAGGAACCCT CTGCCAAGGT GAAAAGCGAG 
TGGTCTGCCG TGAGGGATGA CGGACACAGA GATCCCTGTG CGAAGCTGTG CCCGGCAGAG 
ACTGGTAAAG CCAGCGACAG TTCCAAACCC CTGCCTTCCG GGGGGAGGAC CCAACCCGAT 
TTCTACAAGC AGACCCAGAC TTCGGAGAAA GCATGGGCGC ATGCAAAAAC AAACCACAAA 
GATAGCCAAG ATGAGGTGAA GTCCCTGGCC AGGGAGGACT CAGCTTCACT TTTATATGAA 
AAGGAGATAG GCCGGGCACG AAAAGGTCCT GAACCCAAAC CGGAAGTTCC TGCTACCCGG 
TGCCCTCCTC AGCCACCAGG AATTGAGGGT GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC 
TCTTTGCAGA AACAGGCTCC CAAAGAGCCA GACAGGAAGG AACAGACTTC GCAAAGGCCT 
GGAGGTAGTG GCCCTCAACA ACCCCCACCC ACCAAAGAGC TGTCTAACTC AGCATCCTGG 
CAGCACGGCA GTTCTCCGAG TCACACTTTA AAGAAGGAGC CCGGGACCAA AGCTGCCGCT 
GCAGAACCAA GCACCAGCCT TCATGACACT CCCCGATCTG CTACAGCCAC CACCACTGCC 
ATTGCCACCA CCACCACTAC CACCAGTGCC GGGCACAGTG ACTGCAGTAG CCATAAGGCC 
CGGCCTGGCC CTGACCCCAG CCCTTCAAAG TCTAAGCACC AAGACAGGTC CCTCTCCTCA 
CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC AAAGAGCCTG TCACTCAACC CCTGGGTGGT 
TCCATCAGAG AAGGCAAGGG TGGCAGCAAG GGTCCAGTGG ACACATTTTC TGCTGTCCTG 
ACCACCCAGG GCAAAGCAAG TGATGTGCTT GTGCAGGGAG AAGGTCGGGT CTCAATCATT 
GTCCACACTG AAGAGTGTCC TCTCGATGCC AAACTGAAAA ACACCAATGG AGGGTGTCCC 
CCAGAGATGC AGGCGAAGCA TCCACCCAGA CAAGGACATC TCAGTGAAGC AGCAGACCAG 
AAGCCACTCA TTGCTGGTGA GAAGCAAAGC CCGTCTCCAA AGCATCCCAA ACCATCCACT 
GTGAAAGATT ACCCCAGTCT GTGCAGACAG ACAGACAGAA GCCCAAGCCA TCAGGCTACC 
ACTGGGGACA GGAAGGCAGA AGGAAAGAAA TGCACAGACG CACTTTATGT CGCAGCCCCA 
GAGGGCTACA AGCCAGAGGC CAGCCCTTCT CTCCACCACG GCGAGACCGG ACTCAGAGGC 
TCAGAGAGGC CACCCATGGG CATGGGGAAG GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA 
GGTCCCCAGA AGTCACTGGC TGAAACAGGC AAGCCCAGCG GTATGAAAAG GTCACCCTCT 
GCCACCGTGC AGAGCTCTCT CCGCTCAGCT GCCCCCCCAG AAAAGTCTCT GAGTTACTCA 
GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC 
TCATCTGCCA AGGCTACAGG GGGGACCTCA GAGTTCCCAG CCCCCAGCAG CAGGGACCAC 
AGGAAGCTTC AGTCTGGAGG AGACGGCCGA AGCCAAATGA TAAAGAGTGA CTCTCTGCCC 
TCCTTCCGCC TCTCCACCTC TGCTCTGGAG TCACATTTCC AGGATCCACA GGTGCCCATC 
GCATCAGGCC ACCGAGGCAG GGCACTGTCA GTAACTGCTG CCACAGGAGA ACCCAAAGGG 
AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG AAACAGAATG CGTGCAGAGA GGCGACCAGA 
GCACCCCCAG CCCCAAGCAC AGATCGCTCC CTCCCTCTTT CCTCAGAGAA AGACTTCGTG 
GTTCGGCAGA GAAGGGGCAA GGAGACCTTA AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_006517702.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517765.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X9, mRNA.

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CloneID	OMu49078
Clone ID Related Accession (Same CDS sequence)	XM_006517702.3 , XM_006517702.4
Accession Version	XM_006517702.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517702.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGA AGGAGCTGAG CCTCCCCCGA 
AGAGGAAGTC TGGTAGATTC CCAGAAGTGG AATTGCTTGG TCAAACGTTG CCGAACAAGC 
AACCGGAAGA GTTTGATAGG CAATGGCCAG TCTCCAGCAC TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GACAGTCCAA GGAATTTCTC CCCCAGTGCC 
TCAGCCCATT TCTCATTTGC GAGGAGAAAT GACAGAACGG ATGGGCGCCG CTGGTCTCTG 
GCTTCTCTCC CTTCCTCAGG CTATGGGACA AATACCCCAA GCTCCACCGT CTCCTCATCC 
TGTTCTTCCC AGGAGAAGTT GCACCAGCTA CCATACCAGC CAACCCCAGA TGAATTACAC 
TTCTTATCCA AACACTTCTG CACAACAGAA AGTATCGCCA CTGAGAACCG GTGCAGAAAC 
ACACCCATGC GTCCACGTTC CCGGAGTCTC AGCCCTGGAC GGTCCCCCGC CTGCTGTGAC 
CATGAAATAA TTATGATGAA CCATGTCTAC AAAGAAAGGT TCCCAAAGGC CACAGCTCAG 
ATGGAAGAGC GTCTGAAGGA GATCATCACC AGCTACTCTC CAGACCATGT TCTCCCCTTG 
GCAGATGGGG TACTTAGTTT CACTCACCAT CAGATCATTG AGCTGGCTCG GGACTGTTTG 
GATAAATCTC ACCAGGGTCT CATCACGTCG AGATACTTTT TTGAGTTGCA GCACAAACTG 
GACAAGTTGC TCCAGGAGGC TCACGATCGC TCTGAAAGTG GAGAACTGGC ATTTATCAAG 
CAACTAGTCC GAAAGATCCT AATTGTCATT GCCCGCCCCG CTCGGTTATT GGAGTGTTTG 
GAATTTGATC CTGAAGAATT TTATTACCTA TTGGAAGCTG CAGAAGGCCA TGCCAAAGAA 
GGCCAAGGAA TCAAAACTGA CATCCCTAGG TATATCATCA GCCAGCTGGG ACTCAATAAG 
GACCCTTTAG AAGAAATGGC TCAGTTGGGG AATTACGACA GTAGGACAGC AGAGACACCA 
GAGATGGATG AGTCAGTGAG TAGCTCAAAT ACTTCCCTGA GACTTCGAAG GAAACCCCGA 
GAGAGTGATT TTGAAACAAT TAAATTGATC AGCAATGGAG CCTACGGGGC AGTCTACTTT 
GTTCGGCACA AAGAGTCCCG CCAGAGGTTT GCCATGAAGA AGATCAACAA GCAGAACCTC 
ATCCTTCGGA ACCAGATCCA GCAGGCCTTC GTGGAGCGAG ACATCCTGAC TTTCGCAGAG 
AACCCCTTTG TGGTCAGCAT GTATTGCTCC TTTGAAACGA GGCGTCACTT ATGCATGGTC 
ATGGAGTATG TAGAAGGGGG AGACTGTGCG ACCCTAATGA AAAACATGGG ACCTCTCCCA 
GTTGATATGG CCAGAATGTA TTTCGCCGAG ACCGTCTTGG CCTTGGAGTA CCTGCATAAT 
TACGGAATCG TACACAGGGA CTTGAAGCCA GACAACCTGT TGGTCACCTC CATGGGGCAC 
ATAAAACTGA CTGACTTCGG CTTGTCTAAG GTGGGATTAA TGAGCATGAC CACCAATCTC 
TATGAAGGCC ACATAGAGAA GGACGCTCGA GAGTTCTTAG ATAAACAGGT CTGTGGTACA 
CCTGAGTACA TTGCCCCCGA GGTGATTCTG AGACAGGGCT ACGGGAAACC CGTGGACTGG 
TGGGCCATGG GCATTATCCT CTATGAATTT CTGGTCGGAT GTGTGCCTTT CTTTGGGGAC 
ACTCCAGAAG AGCTATTTGG ACAAGTCATC AGTGATGAAA TCAACTGGCC TGAAAAGGAC 
GAGGCCCCTC CTCCAGACGC TCAGGAGCTG ATTACCTTGC TCCTCAGGCA GAATCCGCTG 
GAGAGGCTGG GAACAGGTGG AGCCTATGAA GTGAAGCAGC ATCGTTTCTT CCGCTCCTTA 
GACTGGAACA GTTTGCTGAG ACAGAAGGCG GAATTTATTC CCCAACTGGA ATCGGAGGAT 
GACACAAGTT ATTTTGATAC TCGGTCAGAG AAGTATCATC ACATGGAGAC GGAGGAGGAG 
GACGATACAA ACGATGAGGA CTTCACCGTG GAGATAAGGC AGTTTTCTTC CTGTTCACAC 
AGGTTTTCAA AAGTTTTCAG CAGTATAGAT CGCATAACTC AAAATTCAGG AGAAGACAAA 
GATGACTCTG AGGACAAGAC CAAAAGCACA ACGTTGCCAT CCACAGAGAC ACTCAGCTGG 
AGTTCCGAAT ACTCTGAAAT GCAACAGTTA TCGACCTCCA ACTCTTCAGA TACTGAAAGC 
AACAGGTGCA AACTCAGCTC TGGCTTGCTC CCCAAGCTGG CTATTTCGAC AGATGGGGAA 
CAAGATGAGG CTGTCCCTTG CTCTGGAGAC CCCAGAGAGG AGCCAGAGAA ACCTGTCCCT 
CCCTCTGAGG AGTGTACTCA GGAGGAGCCC GAGGTCACCA CCCCAGCCAG CACCATCAGC 
AGTTCCACAC TGTCAGTTGG CAGTTTTTCA GAGCACTTGG ATCAGATAAA TGGGCGAAGC 
GAGTGTGTGG ACAGTACAGA TAATTCCTCA AAGCCATCCA GTGAACCCAC TTCTCACGTG 
GCTCGACAGC GCTTAGAAAG CACAGAGAAA AAGAAAATTT CTGGGAAAGT CACAAAGTCC 
CTCTCGGCCA GTGCTCTGTC CCTCATGATC CCAGGAGATA TGTTCGCTGT ATCTCCATTG 
GGAAGCCCAA TGTCCCCACA CTCCCTGTCT TCAGACCCTT CTTCTTCACG GGATTCCTCT 
CCCAGCCGAG ACTCTTCTGC AGCATCTGCC AGTCCGCATC AGCCCATTGT CATCCACAGC 
TCAGGCAAGA ACTATGGGTT CACCATCCGT GCTATCCGCG TGTACGTGGG GGACAGTGAC 
ATCTACACAG TGCACCATAT CGTCTGGAAC GTAGAAGAAG GAAGTCCCGC ATACCAGGCA 
GGACTGAAGG CCGGGGATCT GATCACACAC ATCAACGGAG AGCCGGTGCA CGGCCTCGTC 
CACACGGAAG TTATCGAGCT CCTGCTGAAG AGTGGGAATA AGGTGTCTAT CACCACTACT 
CCATTTGAAA ACACATCAAT CAAAACGGGA CCAGCCAGGA GAAACAGTTA CAAGGGCCGG 
ATGGTGAGAC GAAGCAAGAA GTCCAAGAAG AAGGAGAGTC TAGAAAGGAG GAGATCTCTC 
TTCAAGAAGC TGGCCAAGCA GCCTTCTCCT TTGCTCCACA CCAGCCGAAG TTTCTCCTGC 
TTAAACCGGT CCCTGTCATC TGGAGAGAGC CTCCCGGGTT CCCCAACTCA CAGCTTGTCC 
CCGAGGTCTC CAACACCCAG TTATCGTTCT ACTCCCGATT TTCCGTCAGG TACAAATTCC 
TCCCAGAGCA GCTCCCCAAG TTCAAGTGCC CCCAATTCTC CAGCAGGTTC AGGGCACATC 
CGGCCCAGCA CCCTCCATGG CCTGGCTCCC AAACTCAGCG GGCAGCGATA CCGCTCTGGA 
AGACGGAAGT CGGCTGGCAG CATCCCTCTC TCCCCGCTGG CCAGGACACC CTCTCCCACT 
CCACAGCCTA CCTCTCCTCA GCGTTCACCA TCCCCACTGT TGGGACACTC ACTGGGCAAT 
GCCAAGATCA CTCAGGCCTT TCCTAGCAAG ATGCACTCTC CCCCCACAAT CGTCAGACAC 
ATCGTGAGGC CCAAGAGTGC AGAGCCGCCC CGCTCCCCAC TGCTGAAACG GGTGCAGTCG 
GAGGAAAAGT TGTCACCCTC CTATGGCAGT GACAAGAAGC TTCTGTGCTC CCGCAAGCAT 
AGCCTAGAGG TGACACAAGA GGAGGTACAG AGGGAGCAGT GTCAGCGGGA AGTGACACTG 
CAGAGCCTGG AAGAGAATGT GTGTGACGCT CCTTCCCTCA GTCGGGCCAG GCCAGTGGAG 
CAAGGCTGTC TGAAACGCCC CGTGTCCCGG AAGGTGGGCA GGCAAGAGTC TGTGGATGAC 
CTGGACCGGG ACAAGCTGAA AGCCAAGGTT GTCGTAAAGA AACCAGAAGA GAAACATGAA 
TCGCACCAGA AACCTCACAG CCTTGGTGGT GATTCGGAAA GCTATGCTCT CTTCAGGCTA 
GAGGAGAGAG AGAAAAAAGT GTACTCCAAG GGGTTGGAAA GGTCAGGCCA TTTTGAAAAC 
ACATCAGCAG AGTTGCCTTC TGTGGGCAGC CTGCTGAAGG ACACTCTTCA CAAGCAGGCC 
AGCGTGAGGG CCAGCGAGGG GGTGACCTCA GACGGGGCAG CTTGCAGCCT GACACCAGGG 
GAGCACAGCC AGTCTCTAGG TGACTTTAAG CGGGCCTCAG CTTCTGGCAT TCTCCATGAT 
AGTGTGTGCC CCATCTCTGA TAGGCCTGCT CCTGGAAAGG TTGAATACTC GGAGAAGGCC 
TCTCAGGCCA AAGAGCTCCT TCGAAGTGAA AAACTAGACA GCAAGCTGGC CAATATTGAT 
TACCTCAGAA AGAAAATGTC ATTGGATGAC AAAGATGACA GCCACTGTGC CATCCTGAAA 
CCCAAGATAA CATCTAGCGC CCATGAATGT CTGCCAGGGA ACCCCATACG GCCCATGGCA 
GGGCAACAAG AGACCCCGCC AGCCTCTGAA AACCGGGCAT TCATCAACAG TACCCACACA 
CCTCAGATGA GTGCAGTTTC CTTTGTTCCT CTCAAAGCCT TAGCTGGCCG GGTAGAGAAC 
GGAGGGGAGA AAGCAGGCTT AGCTGCTCCC GAGTCCCCTG TCAGGAAGAG CCCCTCCGAG 
TATAAGCTAG AGGGCAGGTC AGTTTCATGT CTCAAGCCGA TCGAGGGCAC ACTGGACATT 
GCTCTCCTGT CTGGACCTCA CGCCTCCAAA ACAGAGTTGC TTTCCCCAGA GCCTGCACAG 
AGTCCCAGCC CAGGCATCAA CGTGGGACCA TGTGTGCCAC TAGCTCTTCC TGGGAGCAGT 
GGGAAAAAGG GAGACTCCAC CAGCCTGAGA GAGCCTTCCT CAGCCAACTT AAAAGTAAAT 
AAATCTTATC TGCTGGAGCC TCGGTTCCTA CCCCCGAGCC GGGCTCTCCA GGACTCTCTC 
GCAGCCTCTG GGCCAGAACC GAAGTCAAAG CCGGAAAGGA AGCTCATTCA TCCTTCTGCC 
AGGAGCCCAG CAACTGTCAC AGAGAGCAAT CTTCAGCAGA AAGAGGGTGG TCCCGCCACA 
CACCAAGACC GCTCCACTGA CACCAGGAAC CTCCCTGGCC CAGGGCAGAC CCTACACAAT 
GTGGACCTAC CCAGGCTGTG TACACGTGCC CCACTCCCAC CGGAAGGGAC GCCCGCAAAG 
GAGAAGCCAT GTCTGAAGGA ACCCTCTGCC AAGGTGAAAA GCGAGTGGTC TGCCGTGAGG 
GATGACGGAC ACAGAGATCC CTGTGCGAAG CTGTGCCCGG CAGAGACTGG TAAAGCCAGC 
GACAGTTCCA AACCCCTGCC TTCCGGGGGG AGGACCCAAC CCGATTTCTA CAAGCAGACC 
CAGACTTCGG AGAAAGCATG GGCGCATGCA AAAACAAACC ACAAAGATAG CCAAGATGAG 
GTGAAGTCCC TGGCCAGGGA GGACTCAGCT TCACTTTTAT ATGAAAAGGA GATAGGCCGG 
GCACGAAAAG GTCCTGAACC CAAACCGGAA GTTCCTGCTA CCCGGTGCCC TCCTCAGCCA 
CCAGGAATTG AGGGTGAGAA GCGAGAAAAG CTCTCCGCTG CCCCCTCTTT GCAGAAACAG 
GCTCCCAAAG AGCCAGACAG GAAGGAACAG ACTTCGCAAA GGCCTGGAGG TAGTGGCCCT 
CAACAACCCC CACCCACCAA AGAGCTGTCT AACTCAGCAT CCTGGCAGCA CGGCAGTTCT 
CCGAGTCACA CTTTAAAGAA GGAGCCCGGG ACCAAAGCTG CCGCTGCAGA ACCAAGCACC 
AGCCTTCATG ACACTCCCCG ATCTGCTACA GCCACCACCA CTGCCATTGC CACCACCACC 
ACTACCACCA GTGCCGGGCA CAGTGACTGC AGTAGCCATA AGGCCCGGCC TGGCCCTGAC 
CCCAGCCCTT CAAAGTCTAA GCACCAAGAC AGGTCCCTCT CCTCACAGAA GCTGAGTGCT 
GGCTCTGCAA AAGGCAAAGA GCCTGTCACT CAACCCCTGG GTGGTTCCAT CAGAGAAGGC 
AAGGGTGGCA GCAAGGGTCC AGTGGACACA TTTTCTGCTG TCCTGACCAC CCAGGGCAAA 
GCAAGTGATG TGCTTGTGCA GGGAGAAGGT CGGGTCTCAA TCATTGTCCA CACTGAAGAG 
TGTCCTCTCG ATGCCAAACT GAAAAACACC AATGGAGGGT GTCCCCCAGA GATGCAGGCG 
AAGCATCCAC CCAGACAAGG ACATCTCAGT GAAGCAGCAG ACCAGAAGCC ACTCATTGCT 
GGTGAGAAGC AAAGCCCGTC TCCAAAGCAT CCCAAACCAT CCACTGTGAA AGATTACCCC 
AGTCTGTGCA GACAGACAGA CAGAAGCCCA AGCCATCAGG CTACCACTGG GGACAGGAAG 
GCAGAAGGAA AGAAATGCAC AGACGCACTT TATGTCGCAG CCCCAGAGGG CTACAAGCCA 
GAGGCCAGCC CTTCTCTCCA CCACGGCGAG ACCGGACTCA GAGGCTCAGA GAGGCCACCC 
ATGGGCATGG GGAAGGGCTT CTCTGAGCCC AAGGGGAAAG GGCCAGGTCC CCAGAAGTCA 
CTGGCTGAAA CAGGCAAGCC CAGCGGTATG AAAAGGTCAC CCTCTGCCAC CGTGCAGAGC 
TCTCTCCGCT CAGCTGCCCC CCCAGAAAAG TCTCTGAGTT ACTCAGCCAG CTTTCCCGAG 
GCCCAGCCTG GAGTGCGAGA GGTCCCTGCA GCCAACAGCA GCCCCTCATC TGCCAAGGCT 
ACAGGGGGGA CCTCAGAGTT CCCAGCCCCC AGCAGCAGGG ACCACAGGAA GCTTCAGTCT 
GGAGGAGACG GCCGAAGCCA AATGATAAAG AGTGACTCTC TGCCCTCCTT CCGCCTCTCC 
ACCTCTGCTC TGGAGTCACA TTTCCAGGAT CCACAGGTGC CCATCGCATC AGGCCACCGA 
GGCAGGGCAC TGTCAGTAAC TGCTGCCACA GGAGAACCCA AAGGGAGAGA GCTCGCCCAG 
CCTCCCCCAG TCAGGAAACA GAATGCGTGC AGAGAGGCGA CCAGAGCACC CCCAGCCCCA 
AGCACAGATC GCTCCCTCCC TCTTTCCTCA GAGAAAGACT TCGTGGTTCG GCAGAGAAGG 
GGCAAGGAGA CCTTAAGGAG CAGTCCTCAC AAAAAGGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517765.1
CDS	777..8081
Translation	MDESSILRRRGLQKELSLPRRGSLVDSQKWNCLVKRCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_006517702.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517702.3

ORF Insert Sequence:

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGA AGGAGCTGAG CCTCCCCCGA 
AGAGGAAGTC TGGTAGATTC CCAGAAGTGG AATTGCTTGG TCAAACGTTG CCGAACAAGC 
AACCGGAAGA GTTTGATAGG CAATGGCCAG TCTCCAGCAC TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GACAGTCCAA GGAATTTCTC CCCCAGTGCC 
TCAGCCCATT TCTCATTTGC GAGGAGAAAT GACAGAACGG ATGGGCGCCG CTGGTCTCTG 
GCTTCTCTCC CTTCCTCAGG CTATGGGACA AATACCCCAA GCTCCACCGT CTCCTCATCC 
TGTTCTTCCC AGGAGAAGTT GCACCAGCTA CCATACCAGC CAACCCCAGA TGAATTACAC 
TTCTTATCCA AACACTTCTG CACAACAGAA AGTATCGCCA CTGAGAACCG GTGCAGAAAC 
ACACCCATGC GTCCACGTTC CCGGAGTCTC AGCCCTGGAC GGTCCCCCGC CTGCTGTGAC 
CATGAAATAA TTATGATGAA CCATGTCTAC AAAGAAAGGT TCCCAAAGGC CACAGCTCAG 
ATGGAAGAGC GTCTGAAGGA GATCATCACC AGCTACTCTC CAGACCATGT TCTCCCCTTG 
GCAGATGGGG TACTTAGTTT CACTCACCAT CAGATCATTG AGCTGGCTCG GGACTGTTTG 
GATAAATCTC ACCAGGGTCT CATCACGTCG AGATACTTTT TTGAGTTGCA GCACAAACTG 
GACAAGTTGC TCCAGGAGGC TCACGATCGC TCTGAAAGTG GAGAACTGGC ATTTATCAAG 
CAACTAGTCC GAAAGATCCT AATTGTCATT GCCCGCCCCG CTCGGTTATT GGAGTGTTTG 
GAATTTGATC CTGAAGAATT TTATTACCTA TTGGAAGCTG CAGAAGGCCA TGCCAAAGAA 
GGCCAAGGAA TCAAAACTGA CATCCCTAGG TATATCATCA GCCAGCTGGG ACTCAATAAG 
GACCCTTTAG AAGAAATGGC TCAGTTGGGG AATTACGACA GTAGGACAGC AGAGACACCA 
GAGATGGATG AGTCAGTGAG TAGCTCAAAT ACTTCCCTGA GACTTCGAAG GAAACCCCGA 
GAGAGTGATT TTGAAACAAT TAAATTGATC AGCAATGGAG CCTACGGGGC AGTCTACTTT 
GTTCGGCACA AAGAGTCCCG CCAGAGGTTT GCCATGAAGA AGATCAACAA GCAGAACCTC 
ATCCTTCGGA ACCAGATCCA GCAGGCCTTC GTGGAGCGAG ACATCCTGAC TTTCGCAGAG 
AACCCCTTTG TGGTCAGCAT GTATTGCTCC TTTGAAACGA GGCGTCACTT ATGCATGGTC 
ATGGAGTATG TAGAAGGGGG AGACTGTGCG ACCCTAATGA AAAACATGGG ACCTCTCCCA 
GTTGATATGG CCAGAATGTA TTTCGCCGAG ACCGTCTTGG CCTTGGAGTA CCTGCATAAT 
TACGGAATCG TACACAGGGA CTTGAAGCCA GACAACCTGT TGGTCACCTC CATGGGGCAC 
ATAAAACTGA CTGACTTCGG CTTGTCTAAG GTGGGATTAA TGAGCATGAC CACCAATCTC 
TATGAAGGCC ACATAGAGAA GGACGCTCGA GAGTTCTTAG ATAAACAGGT CTGTGGTACA 
CCTGAGTACA TTGCCCCCGA GGTGATTCTG AGACAGGGCT ACGGGAAACC CGTGGACTGG 
TGGGCCATGG GCATTATCCT CTATGAATTT CTGGTCGGAT GTGTGCCTTT CTTTGGGGAC 
ACTCCAGAAG AGCTATTTGG ACAAGTCATC AGTGATGAAA TCAACTGGCC TGAAAAGGAC 
GAGGCCCCTC CTCCAGACGC TCAGGAGCTG ATTACCTTGC TCCTCAGGCA GAATCCGCTG 
GAGAGGCTGG GAACAGGTGG AGCCTATGAA GTGAAGCAGC ATCGTTTCTT CCGCTCCTTA 
GACTGGAACA GTTTGCTGAG ACAGAAGGCG GAATTTATTC CCCAACTGGA ATCGGAGGAT 
GACACAAGTT ATTTTGATAC TCGGTCAGAG AAGTATCATC ACATGGAGAC GGAGGAGGAG 
GACGATACAA ACGATGAGGA CTTCACCGTG GAGATAAGGC AGTTTTCTTC CTGTTCACAC 
AGGTTTTCAA AAGTTTTCAG CAGTATAGAT CGCATAACTC AAAATTCAGG AGAAGACAAA 
GATGACTCTG AGGACAAGAC CAAAAGCACA ACGTTGCCAT CCACAGAGAC ACTCAGCTGG 
AGTTCCGAAT ACTCTGAAAT GCAACAGTTA TCGACCTCCA ACTCTTCAGA TACTGAAAGC 
AACAGGTGCA AACTCAGCTC TGGCTTGCTC CCCAAGCTGG CTATTTCGAC AGATGGGGAA 
CAAGATGAGG CTGTCCCTTG CTCTGGAGAC CCCAGAGAGG AGCCAGAGAA ACCTGTCCCT 
CCCTCTGAGG AGTGTACTCA GGAGGAGCCC GAGGTCACCA CCCCAGCCAG CACCATCAGC 
AGTTCCACAC TGTCAGTTGG CAGTTTTTCA GAGCACTTGG ATCAGATAAA TGGGCGAAGC 
GAGTGTGTGG ACAGTACAGA TAATTCCTCA AAGCCATCCA GTGAACCCAC TTCTCACGTG 
GCTCGACAGC GCTTAGAAAG CACAGAGAAA AAGAAAATTT CTGGGAAAGT CACAAAGTCC 
CTCTCGGCCA GTGCTCTGTC CCTCATGATC CCAGGAGATA TGTTCGCTGT ATCTCCATTG 
GGAAGCCCAA TGTCCCCACA CTCCCTGTCT TCAGACCCTT CTTCTTCACG GGATTCCTCT 
CCCAGCCGAG ACTCTTCTGC AGCATCTGCC AGTCCGCATC AGCCCATTGT CATCCACAGC 
TCAGGCAAGA ACTATGGGTT CACCATCCGT GCTATCCGCG TGTACGTGGG GGACAGTGAC 
ATCTACACAG TGCACCATAT CGTCTGGAAC GTAGAAGAAG GAAGTCCCGC ATACCAGGCA 
GGACTGAAGG CCGGGGATCT GATCACACAC ATCAACGGAG AGCCGGTGCA CGGCCTCGTC 
CACACGGAAG TTATCGAGCT CCTGCTGAAG AGTGGGAATA AGGTGTCTAT CACCACTACT 
CCATTTGAAA ACACATCAAT CAAAACGGGA CCAGCCAGGA GAAACAGTTA CAAGGGCCGG 
ATGGTGAGAC GAAGCAAGAA GTCCAAGAAG AAGGAGAGTC TAGAAAGGAG GAGATCTCTC 
TTCAAGAAGC TGGCCAAGCA GCCTTCTCCT TTGCTCCACA CCAGCCGAAG TTTCTCCTGC 
TTAAACCGGT CCCTGTCATC TGGAGAGAGC CTCCCGGGTT CCCCAACTCA CAGCTTGTCC 
CCGAGGTCTC CAACACCCAG TTATCGTTCT ACTCCCGATT TTCCGTCAGG TACAAATTCC 
TCCCAGAGCA GCTCCCCAAG TTCAAGTGCC CCCAATTCTC CAGCAGGTTC AGGGCACATC 
CGGCCCAGCA CCCTCCATGG CCTGGCTCCC AAACTCAGCG GGCAGCGATA CCGCTCTGGA 
AGACGGAAGT CGGCTGGCAG CATCCCTCTC TCCCCGCTGG CCAGGACACC CTCTCCCACT 
CCACAGCCTA CCTCTCCTCA GCGTTCACCA TCCCCACTGT TGGGACACTC ACTGGGCAAT 
GCCAAGATCA CTCAGGCCTT TCCTAGCAAG ATGCACTCTC CCCCCACAAT CGTCAGACAC 
ATCGTGAGGC CCAAGAGTGC AGAGCCGCCC CGCTCCCCAC TGCTGAAACG GGTGCAGTCG 
GAGGAAAAGT TGTCACCCTC CTATGGCAGT GACAAGAAGC TTCTGTGCTC CCGCAAGCAT 
AGCCTAGAGG TGACACAAGA GGAGGTACAG AGGGAGCAGT GTCAGCGGGA AGTGACACTG 
CAGAGCCTGG AAGAGAATGT GTGTGACGCT CCTTCCCTCA GTCGGGCCAG GCCAGTGGAG 
CAAGGCTGTC TGAAACGCCC CGTGTCCCGG AAGGTGGGCA GGCAAGAGTC TGTGGATGAC 
CTGGACCGGG ACAAGCTGAA AGCCAAGGTT GTCGTAAAGA AACCAGAAGA GAAACATGAA 
TCGCACCAGA AACCTCACAG CCTTGGTGGT GATTCGGAAA GCTATGCTCT CTTCAGGCTA 
GAGGAGAGAG AGAAAAAAGT GTACTCCAAG GGGTTGGAAA GGTCAGGCCA TTTTGAAAAC 
ACATCAGCAG AGTTGCCTTC TGTGGGCAGC CTGCTGAAGG ACACTCTTCA CAAGCAGGCC 
AGCGTGAGGG CCAGCGAGGG GGTGACCTCA GACGGGGCAG CTTGCAGCCT GACACCAGGG 
GAGCACAGCC AGTCTCTAGG TGACTTTAAG CGGGCCTCAG CTTCTGGCAT TCTCCATGAT 
AGTGTGTGCC CCATCTCTGA TAGGCCTGCT CCTGGAAAGG TTGAATACTC GGAGAAGGCC 
TCTCAGGCCA AAGAGCTCCT TCGAAGTGAA AAACTAGACA GCAAGCTGGC CAATATTGAT 
TACCTCAGAA AGAAAATGTC ATTGGATGAC AAAGATGACA GCCACTGTGC CATCCTGAAA 
CCCAAGATAA CATCTAGCGC CCATGAATGT CTGCCAGGGA ACCCCATACG GCCCATGGCA 
GGGCAACAAG AGACCCCGCC AGCCTCTGAA AACCGGGCAT TCATCAACAG TACCCACACA 
CCTCAGATGA GTGCAGTTTC CTTTGTTCCT CTCAAAGCCT TAGCTGGCCG GGTAGAGAAC 
GGAGGGGAGA AAGCAGGCTT AGCTGCTCCC GAGTCCCCTG TCAGGAAGAG CCCCTCCGAG 
TATAAGCTAG AGGGCAGGTC AGTTTCATGT CTCAAGCCGA TCGAGGGCAC ACTGGACATT 
GCTCTCCTGT CTGGACCTCA CGCCTCCAAA ACAGAGTTGC TTTCCCCAGA GCCTGCACAG 
AGTCCCAGCC CAGGCATCAA CGTGGGACCA TGTGTGCCAC TAGCTCTTCC TGGGAGCAGT 
GGGAAAAAGG GAGACTCCAC CAGCCTGAGA GAGCCTTCCT CAGCCAACTT AAAAGTAAAT 
AAATCTTATC TGCTGGAGCC TCGGTTCCTA CCCCCGAGCC GGGCTCTCCA GGACTCTCTC 
GCAGCCTCTG GGCCAGAACC GAAGTCAAAG CCGGAAAGGA AGCTCATTCA TCCTTCTGCC 
AGGAGCCCAG CAACTGTCAC AGAGAGCAAT CTTCAGCAGA AAGAGGGTGG TCCCGCCACA 
CACCAAGACC GCTCCACTGA CACCAGGAAC CTCCCTGGCC CAGGGCAGAC CCTACACAAT 
GTGGACCTAC CCAGGCTGTG TACACGTGCC CCACTCCCAC CGGAAGGGAC GCCCGCAAAG 
GAGAAGCCAT GTCTGAAGGA ACCCTCTGCC AAGGTGAAAA GCGAGTGGTC TGCCGTGAGG 
GATGACGGAC ACAGAGATCC CTGTGCGAAG CTGTGCCCGG CAGAGACTGG TAAAGCCAGC 
GACAGTTCCA AACCCCTGCC TTCCGGGGGG AGGACCCAAC CCGATTTCTA CAAGCAGACC 
CAGACTTCGG AGAAAGCATG GGCGCATGCA AAAACAAACC ACAAAGATAG CCAAGATGAG 
GTGAAGTCCC TGGCCAGGGA GGACTCAGCT TCACTTTTAT ATGAAAAGGA GATAGGCCGG 
GCACGAAAAG GTCCTGAACC CAAACCGGAA GTTCCTGCTA CCCGGTGCCC TCCTCAGCCA 
CCAGGAATTG AGGGTGAGAA GCGAGAAAAG CTCTCCGCTG CCCCCTCTTT GCAGAAACAG 
GCTCCCAAAG AGCCAGACAG GAAGGAACAG ACTTCGCAAA GGCCTGGAGG TAGTGGCCCT 
CAACAACCCC CACCCACCAA AGAGCTGTCT AACTCAGCAT CCTGGCAGCA CGGCAGTTCT 
CCGAGTCACA CTTTAAAGAA GGAGCCCGGG ACCAAAGCTG CCGCTGCAGA ACCAAGCACC 
AGCCTTCATG ACACTCCCCG ATCTGCTACA GCCACCACCA CTGCCATTGC CACCACCACC 
ACTACCACCA GTGCCGGGCA CAGTGACTGC AGTAGCCATA AGGCCCGGCC TGGCCCTGAC 
CCCAGCCCTT CAAAGTCTAA GCACCAAGAC AGGTCCCTCT CCTCACAGAA GCTGAGTGCT 
GGCTCTGCAA AAGGCAAAGA GCCTGTCACT CAACCCCTGG GTGGTTCCAT CAGAGAAGGC 
AAGGGTGGCA GCAAGGGTCC AGTGGACACA TTTTCTGCTG TCCTGACCAC CCAGGGCAAA 
GCAAGTGATG TGCTTGTGCA GGGAGAAGGT CGGGTCTCAA TCATTGTCCA CACTGAAGAG 
TGTCCTCTCG ATGCCAAACT GAAAAACACC AATGGAGGGT GTCCCCCAGA GATGCAGGCG 
AAGCATCCAC CCAGACAAGG ACATCTCAGT GAAGCAGCAG ACCAGAAGCC ACTCATTGCT 
GGTGAGAAGC AAAGCCCGTC TCCAAAGCAT CCCAAACCAT CCACTGTGAA AGATTACCCC 
AGTCTGTGCA GACAGACAGA CAGAAGCCCA AGCCATCAGG CTACCACTGG GGACAGGAAG 
GCAGAAGGAA AGAAATGCAC AGACGCACTT TATGTCGCAG CCCCAGAGGG CTACAAGCCA 
GAGGCCAGCC CTTCTCTCCA CCACGGCGAG ACCGGACTCA GAGGCTCAGA GAGGCCACCC 
ATGGGCATGG GGAAGGGCTT CTCTGAGCCC AAGGGGAAAG GGCCAGGTCC CCAGAAGTCA 
CTGGCTGAAA CAGGCAAGCC CAGCGGTATG AAAAGGTCAC CCTCTGCCAC CGTGCAGAGC 
TCTCTCCGCT CAGCTGCCCC CCCAGAAAAG TCTCTGAGTT ACTCAGCCAG CTTTCCCGAG 
GCCCAGCCTG GAGTGCGAGA GGTCCCTGCA GCCAACAGCA GCCCCTCATC TGCCAAGGCT 
ACAGGGGGGA CCTCAGAGTT CCCAGCCCCC AGCAGCAGGG ACCACAGGAA GCTTCAGTCT 
GGAGGAGACG GCCGAAGCCA AATGATAAAG AGTGACTCTC TGCCCTCCTT CCGCCTCTCC 
ACCTCTGCTC TGGAGTCACA TTTCCAGGAT CCACAGGTGC CCATCGCATC AGGCCACCGA 
GGCAGGGCAC TGTCAGTAAC TGCTGCCACA GGAGAACCCA AAGGGAGAGA GCTCGCCCAG 
CCTCCCCCAG TCAGGAAACA GAATGCGTGC AGAGAGGCGA CCAGAGCACC CCCAGCCCCA 
AGCACAGATC GCTCCCTCCC TCTTTCCTCA GAGAAAGACT TCGTGGTTCG GCAGAGAAGG 
GGCAAGGAGA CCTTAAGGAG CAGTCCTCAC AAAAAGGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_006517703.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517766.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X10, mRNA.

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CloneID	OMu49079
Clone ID Related Accession (Same CDS sequence)	XM_006517703.4 , XM_006517703.3
Accession Version	XM_006517703.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATGT CTGACCCCAA TTTCTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGCACACAA AGTTGCCGAA CAAGCAACCG GAAGAGTTTG 
ATAGGCAATG GCCAGTCTCC AGCACTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGACAG TCCAAGGAAT TTCTCCCCCA GTGCCTCAGC CCATTTCTCA 
TTTGCGAGGA GAAATGACAG AACGGATGGG CGCCGCTGGT CTCTGGCTTC TCTCCCTTCC 
TCAGGCTATG GGACAAATAC CCCAAGCTCC ACCGTCTCCT CATCCTGTTC TTCCCAGGAG 
AAGTTGCACC AGCTACCATA CCAGCCAACC CCAGATGAAT TACACTTCTT ATCCAAACAC 
TTCTGCACAA CAGAAAGTAT CGCCACTGAG AACCGGTGCA GAAACACACC CATGCGTCCA 
CGTTCCCGGA GTCTCAGCCC TGGACGGTCC CCCGCCTGCT GTGACCATGA AATAATTATG 
ATGAACCATG TCTACAAAGA AAGGTTCCCA AAGGCCACAG CTCAGATGGA AGAGCGTCTG 
AAGGAGATCA TCACCAGCTA CTCTCCAGAC CATGTTCTCC CCTTGGCAGA TGGGGTACTT 
AGTTTCACTC ACCATCAGAT CATTGAGCTG GCTCGGGACT GTTTGGATAA ATCTCACCAG 
GGTCTCATCA CGTCGAGATA CTTTTTTGAG TTGCAGCACA AACTGGACAA GTTGCTCCAG 
GAGGCTCACG ATCGCTCTGA AAGTGGAGAA CTGGCATTTA TCAAGCAACT AGTCCGAAAG 
ATCCTAATTG TCATTGCCCG CCCCGCTCGG TTATTGGAGT GTTTGGAATT TGATCCTGAA 
GAATTTTATT ACCTATTGGA AGCTGCAGAA GGCCATGCCA AAGAAGGCCA AGGAATCAAA 
ACTGACATCC CTAGGTATAT CATCAGCCAG CTGGGACTCA ATAAGGACCC TTTAGAAGAA 
ATGGCTCAGT TGGGGAATTA CGACAGTAGG ACAGCAGAGA CACCAGAGAT GGATGAGTCA 
GTGAGTAGCT CAAATACTTC CCTGAGACTT CGAAGGAAAC CCCGAGAGAG TGATTTTGAA 
ACAATTAAAT TGATCAGCAA TGGAGCCTAC GGGGCAGTCT ACTTTGTTCG GCACAAAGAG 
TCCCGCCAGA GGTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTCATCCT TCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGAGACATC CTGACTTTCG CAGAGAACCC CTTTGTGGTC 
AGCATGTATT GCTCCTTTGA AACGAGGCGT CACTTATGCA TGGTCATGGA GTATGTAGAA 
GGGGGAGACT GTGCGACCCT AATGAAAAAC ATGGGACCTC TCCCAGTTGA TATGGCCAGA 
ATGTATTTCG CCGAGACCGT CTTGGCCTTG GAGTACCTGC ATAATTACGG AATCGTACAC 
AGGGACTTGA AGCCAGACAA CCTGTTGGTC ACCTCCATGG GGCACATAAA ACTGACTGAC 
TTCGGCTTGT CTAAGGTGGG ATTAATGAGC ATGACCACCA ATCTCTATGA AGGCCACATA 
GAGAAGGACG CTCGAGAGTT CTTAGATAAA CAGGTCTGTG GTACACCTGA GTACATTGCC 
CCCGAGGTGA TTCTGAGACA GGGCTACGGG AAACCCGTGG ACTGGTGGGC CATGGGCATT 
ATCCTCTATG AATTTCTGGT CGGATGTGTG CCTTTCTTTG GGGACACTCC AGAAGAGCTA 
TTTGGACAAG TCATCAGTGA TGAAATCAAC TGGCCTGAAA AGGACGAGGC CCCTCCTCCA 
GACGCTCAGG AGCTGATTAC CTTGCTCCTC AGGCAGAATC CGCTGGAGAG GCTGGGAACA 
GGTGGAGCCT ATGAAGTGAA GCAGCATCGT TTCTTCCGCT CCTTAGACTG GAACAGTTTG 
CTGAGACAGA AGGCGGAATT TATTCCCCAA CTGGAATCGG AGGATGACAC AAGTTATTTT 
GATACTCGGT CAGAGAAGTA TCATCACATG GAGACGGAGG AGGAGGACGA TACAAACGAT 
GAGGACTTCA CCGTGGAGAT AAGGCAGTTT TCTTCCTGTT CACACAGGTT TTCAAAAGTT 
TTCAGCAGTA TAGATCGCAT AACTCAAAAT TCAGGAGAAG ACAAAGATGA CTCTGAGGAC 
AAGACCAAAA GCACAACGTT GCCATCCACA GAGACACTCA GCTGGAGTTC CGAATACTCT 
GAAATGCAAC AGTTATCGAC CTCCAACTCT TCAGATACTG AAAGCAACAG GTGCAAACTC 
AGCTCTGGCT TGCTCCCCAA GCTGGCTATT TCGACAGATG GGGAACAAGA TGAGGCTGTC 
CCTTGCTCTG GAGACCCCAG AGAGGAGCCA GAGAAACCTG TCCCTCCCTC TGAGGAGTGT 
ACTCAGGAGG AGCCCGAGGT CACCACCCCA GCCAGCACCA TCAGCAGTTC CACACTGTCA 
GTTGGCAGTT TTTCAGAGCA CTTGGATCAG ATAAATGGGC GAAGCGAGTG TGTGGACAGT 
ACAGATAATT CCTCAAAGCC ATCCAGTGAA CCCACTTCTC ACGTGGCTCG ACAGCGCTTA 
GAAAGCACAG AGAAAAAGAA AATTTCTGGG AAAGTCACAA AGTCCCTCTC GGCCAGTGCT 
CTGTCCCTCA TGATCCCAGG AGATATGTTC GCTGTATCTC CATTGGGAAG CCCAATGTCC 
CCACACTCCC TGTCTTCAGA CCCTTCTTCT TCACGGGATT CCTCTCCCAG CCGAGACTCT 
TCTGCAGCAT CTGCCAGTCC GCATCAGCCC ATTGTCATCC ACAGCTCAGG CAAGAACTAT 
GGGTTCACCA TCCGTGCTAT CCGCGTGTAC GTGGGGGACA GTGACATCTA CACAGTGCAC 
CATATCGTCT GGAACGTAGA AGAAGGAAGT CCCGCATACC AGGCAGGACT GAAGGCCGGG 
GATCTGATCA CACACATCAA CGGAGAGCCG GTGCACGGCC TCGTCCACAC GGAAGTTATC 
GAGCTCCTGC TGAAGAGTGG GAATAAGGTG TCTATCACCA CTACTCCATT TGAAAACACA 
TCAATCAAAA CGGGACCAGC CAGGAGAAAC AGTTACAAGG GCCGGATGGT GAGACGAAGC 
AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC 
AAGCAGCCTT CTCCTTTGCT CCACACCAGC CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG 
TCATCTGGAG AGAGCCTCCC GGGTTCCCCA ACTCACAGCT TGTCCCCGAG GTCTCCAACA 
CCCAGTTATC GTTCTACTCC CGATTTTCCG TCAGGTACAA ATTCCTCCCA GAGCAGCTCC 
CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA GGTTCAGGGC ACATCCGGCC CAGCACCCTC 
CATGGCCTGG CTCCCAAACT CAGCGGGCAG CGATACCGCT CTGGAAGACG GAAGTCGGCT 
GGCAGCATCC CTCTCTCCCC GCTGGCCAGG ACACCCTCTC CCACTCCACA GCCTACCTCT 
CCTCAGCGTT CACCATCCCC ACTGTTGGGA CACTCACTGG GCAATGCCAA GATCACTCAG 
GCCTTTCCTA GCAAGATGCA CTCTCCCCCC ACAATCGTCA GACACATCGT GAGGCCCAAG 
AGTGCAGAGC CGCCCCGCTC CCCACTGCTG AAACGGGTGC AGTCGGAGGA AAAGTTGTCA 
CCCTCCTATG GCAGTGACAA GAAGCTTCTG TGCTCCCGCA AGCATAGCCT AGAGGTGACA 
CAAGAGGAGG TACAGAGGGA GCAGTGTCAG CGGGAAGTGA CACTGCAGAG CCTGGAAGAG 
AATGTGTGTG ACGCTCCTTC CCTCAGTCGG GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA 
CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA GAGTCTGTGG ATGACCTGGA CCGGGACAAG 
CTGAAAGCCA AGGTTGTCGT AAAGAAACCA GAAGAGAAAC ATGAATCGCA CCAGAAACCT 
CACAGCCTTG GTGGTGATTC GGAAAGCTAT GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA 
AAAGTGTACT CCAAGGGGTT GGAAAGGTCA GGCCATTTTG AAAACACATC AGCAGAGTTG 
CCTTCTGTGG GCAGCCTGCT GAAGGACACT CTTCACAAGC AGGCCAGCGT GAGGGCCAGC 
GAGGGGGTGA CCTCAGACGG GGCAGCTTGC AGCCTGACAC CAGGGGAGCA CAGCCAGTCT 
CTAGGTGACT TTAAGCGGGC CTCAGCTTCT GGCATTCTCC ATGATAGTGT GTGCCCCATC 
TCTGATAGGC CTGCTCCTGG AAAGGTTGAA TACTCGGAGA AGGCCTCTCA GGCCAAAGAG 
CTCCTTCGAA GTGAAAAACT AGACAGCAAG CTGGCCAATA TTGATTACCT CAGAAAGAAA 
ATGTCATTGG ATGACAAAGA TGACAGCCAC TGTGCCATCC TGAAACCCAA GATAACATCT 
AGCGCCCATG AATGTCTGCC AGGGAACCCC ATACGGCCCA TGGCAGGGCA ACAAGAGACC 
CCGCCAGCCT CTGAAAACCG GGCATTCATC AACAGTACCC ACACACCTCA GATGAGTGCA 
GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT GGCCGGGTAG AGAACGGAGG GGAGAAAGCA 
GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG AAGAGCCCCT CCGAGTATAA GCTAGAGGGC 
AGGTCAGTTT CATGTCTCAA GCCGATCGAG GGCACACTGG ACATTGCTCT CCTGTCTGGA 
CCTCACGCCT CCAAAACAGA GTTGCTTTCC CCAGAGCCTG CACAGAGTCC CAGCCCAGGC 
ATCAACGTGG GACCATGTGT GCCACTAGCT CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC 
TCCACCAGCC TGAGAGAGCC TTCCTCAGCC AACTTAAAAG TAAATAAATC TTATCTGCTG 
GAGCCTCGGT TCCTACCCCC GAGCCGGGCT CTCCAGGACT CTCTCGCAGC CTCTGGGCCA 
GAACCGAAGT CAAAGCCGGA AAGGAAGCTC ATTCATCCTT CTGCCAGGAG CCCAGCAACT 
GTCACAGAGA GCAATCTTCA GCAGAAAGAG GGTGGTCCCG CCACACACCA AGACCGCTCC 
ACTGACACCA GGAACCTCCC TGGCCCAGGG CAGACCCTAC ACAATGTGGA CCTACCCAGG 
CTGTGTACAC GTGCCCCACT CCCACCGGAA GGGACGCCCG CAAAGGAGAA GCCATGTCTG 
AAGGAACCCT CTGCCAAGGT GAAAAGCGAG TGGTCTGCCG TGAGGGATGA CGGACACAGA 
GATCCCTGTG CGAAGCTGTG CCCGGCAGAG ACTGGTAAAG CCAGCGACAG TTCCAAACCC 
CTGCCTTCCG GGGGGAGGAC CCAACCCGAT TTCTACAAGC AGACCCAGAC TTCGGAGAAA 
GCATGGGCGC ATGCAAAAAC AAACCACAAA GATAGCCAAG ATGAGGTGAA GTCCCTGGCC 
AGGGAGGACT CAGCTTCACT TTTATATGAA AAGGAGATAG GCCGGGCACG AAAAGGTCCT 
GAACCCAAAC CGGAAGTTCC TGCTACCCGG TGCCCTCCTC AGCCACCAGG AATTGAGGGT 
GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC TCTTTGCAGA AACAGGCTCC CAAAGAGCCA 
GACAGGAAGG AACAGACTTC GCAAAGGCCT GGAGGTAGTG GCCCTCAACA ACCCCCACCC 
ACCAAAGAGC TGTCTAACTC AGCATCCTGG CAGCACGGCA GTTCTCCGAG TCACACTTTA 
AAGAAGGAGC CCGGGACCAA AGCTGCCGCT GCAGAACCAA GCACCAGCCT TCATGACACT 
CCCCGATCTG CTACAGCCAC CACCACTGCC ATTGCCACCA CCACCACTAC CACCAGTGCC 
GGGCACAGTG ACTGCAGTAG CCATAAGGCC CGGCCTGGCC CTGACCCCAG CCCTTCAAAG 
TCTAAGCACC AAGACAGGTC CCTCTCCTCA CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC 
AAAGAGCCTG TCACTCAACC CCTGGGTGGT TCCATCAGAG AAGGCAAGGG TGGCAGCAAG 
GGTCCAGTGG ACACATTTTC TGCTGTCCTG ACCACCCAGG GCAAAGCAAG TGATGTGCTT 
GTGCAGGGAG AAGGTCGGGT CTCAATCATT GTCCACACTG AAGAGTGTCC TCTCGATGCC 
AAACTGAAAA ACACCAATGG AGGGTGTCCC CCAGAGATGC AGGCGAAGCA TCCACCCAGA 
CAAGGACATC TCAGTGAAGC AGCAGACCAG AAGCCACTCA TTGCTGGTGA GAAGCAAAGC 
CCGTCTCCAA AGCATCCCAA ACCATCCACT GTGAAAGATT ACCCCAGTCT GTGCAGACAG 
ACAGACAGAA GCCCAAGCCA TCAGGCTACC ACTGGGGACA GGAAGGCAGA AGGAAAGAAA 
TGCACAGACG CACTTTATGT CGCAGCCCCA GAGGGCTACA AGCCAGAGGC CAGCCCTTCT 
CTCCACCACG GCGAGACCGG ACTCAGAGGC TCAGAGAGGC CACCCATGGG CATGGGGAAG 
GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA GGTCCCCAGA AGTCACTGGC TGAAACAGGC 
AAGCCCAGCG GTATGAAAAG GTCACCCTCT GCCACCGTGC AGAGCTCTCT CCGCTCAGCT 
GCCCCCCCAG AAAAGTCTCT GAGTTACTCA GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG 
CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC TCATCTGCCA AGGCTACAGG GGGGACCTCA 
GAGTTCCCAG CCCCCAGCAG CAGGGACCAC AGGAAGCTTC AGTCTGGAGG AGACGGCCGA 
AGCCAAATGA TAAAGAGTGA CTCTCTGCCC TCCTTCCGCC TCTCCACCTC TGCTCTGGAG 
TCACATTTCC AGGATCCACA GGTGCCCATC GCATCAGGCC ACCGAGGCAG GGCACTGTCA 
GTAACTGCTG CCACAGGAGA ACCCAAAGGG AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG 
AAACAGAATG CGTGCAGAGA GGCGACCAGA GCACCCCCAG CCCCAAGCAC AGATCGCTCC 
CTCCCTCTTT CCTCAGAGAA AGACTTCGTG GTTCGGCAGA GAAGGGGCAA GGAGACCTTA 
AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517766.1
CDS	1234..8523
Translation	MDMSDPNFWTVLSNFTLPHLRSGNRLRRTQSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_006517703.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517703.3

ORF Insert Sequence:

ATGGATATGT CTGACCCCAA TTTCTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGCACACAA AGTTGCCGAA CAAGCAACCG GAAGAGTTTG 
ATAGGCAATG GCCAGTCTCC AGCACTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGACAG TCCAAGGAAT TTCTCCCCCA GTGCCTCAGC CCATTTCTCA 
TTTGCGAGGA GAAATGACAG AACGGATGGG CGCCGCTGGT CTCTGGCTTC TCTCCCTTCC 
TCAGGCTATG GGACAAATAC CCCAAGCTCC ACCGTCTCCT CATCCTGTTC TTCCCAGGAG 
AAGTTGCACC AGCTACCATA CCAGCCAACC CCAGATGAAT TACACTTCTT ATCCAAACAC 
TTCTGCACAA CAGAAAGTAT CGCCACTGAG AACCGGTGCA GAAACACACC CATGCGTCCA 
CGTTCCCGGA GTCTCAGCCC TGGACGGTCC CCCGCCTGCT GTGACCATGA AATAATTATG 
ATGAACCATG TCTACAAAGA AAGGTTCCCA AAGGCCACAG CTCAGATGGA AGAGCGTCTG 
AAGGAGATCA TCACCAGCTA CTCTCCAGAC CATGTTCTCC CCTTGGCAGA TGGGGTACTT 
AGTTTCACTC ACCATCAGAT CATTGAGCTG GCTCGGGACT GTTTGGATAA ATCTCACCAG 
GGTCTCATCA CGTCGAGATA CTTTTTTGAG TTGCAGCACA AACTGGACAA GTTGCTCCAG 
GAGGCTCACG ATCGCTCTGA AAGTGGAGAA CTGGCATTTA TCAAGCAACT AGTCCGAAAG 
ATCCTAATTG TCATTGCCCG CCCCGCTCGG TTATTGGAGT GTTTGGAATT TGATCCTGAA 
GAATTTTATT ACCTATTGGA AGCTGCAGAA GGCCATGCCA AAGAAGGCCA AGGAATCAAA 
ACTGACATCC CTAGGTATAT CATCAGCCAG CTGGGACTCA ATAAGGACCC TTTAGAAGAA 
ATGGCTCAGT TGGGGAATTA CGACAGTAGG ACAGCAGAGA CACCAGAGAT GGATGAGTCA 
GTGAGTAGCT CAAATACTTC CCTGAGACTT CGAAGGAAAC CCCGAGAGAG TGATTTTGAA 
ACAATTAAAT TGATCAGCAA TGGAGCCTAC GGGGCAGTCT ACTTTGTTCG GCACAAAGAG 
TCCCGCCAGA GGTTTGCCAT GAAGAAGATC AACAAGCAGA ACCTCATCCT TCGGAACCAG 
ATCCAGCAGG CCTTCGTGGA GCGAGACATC CTGACTTTCG CAGAGAACCC CTTTGTGGTC 
AGCATGTATT GCTCCTTTGA AACGAGGCGT CACTTATGCA TGGTCATGGA GTATGTAGAA 
GGGGGAGACT GTGCGACCCT AATGAAAAAC ATGGGACCTC TCCCAGTTGA TATGGCCAGA 
ATGTATTTCG CCGAGACCGT CTTGGCCTTG GAGTACCTGC ATAATTACGG AATCGTACAC 
AGGGACTTGA AGCCAGACAA CCTGTTGGTC ACCTCCATGG GGCACATAAA ACTGACTGAC 
TTCGGCTTGT CTAAGGTGGG ATTAATGAGC ATGACCACCA ATCTCTATGA AGGCCACATA 
GAGAAGGACG CTCGAGAGTT CTTAGATAAA CAGGTCTGTG GTACACCTGA GTACATTGCC 
CCCGAGGTGA TTCTGAGACA GGGCTACGGG AAACCCGTGG ACTGGTGGGC CATGGGCATT 
ATCCTCTATG AATTTCTGGT CGGATGTGTG CCTTTCTTTG GGGACACTCC AGAAGAGCTA 
TTTGGACAAG TCATCAGTGA TGAAATCAAC TGGCCTGAAA AGGACGAGGC CCCTCCTCCA 
GACGCTCAGG AGCTGATTAC CTTGCTCCTC AGGCAGAATC CGCTGGAGAG GCTGGGAACA 
GGTGGAGCCT ATGAAGTGAA GCAGCATCGT TTCTTCCGCT CCTTAGACTG GAACAGTTTG 
CTGAGACAGA AGGCGGAATT TATTCCCCAA CTGGAATCGG AGGATGACAC AAGTTATTTT 
GATACTCGGT CAGAGAAGTA TCATCACATG GAGACGGAGG AGGAGGACGA TACAAACGAT 
GAGGACTTCA CCGTGGAGAT AAGGCAGTTT TCTTCCTGTT CACACAGGTT TTCAAAAGTT 
TTCAGCAGTA TAGATCGCAT AACTCAAAAT TCAGGAGAAG ACAAAGATGA CTCTGAGGAC 
AAGACCAAAA GCACAACGTT GCCATCCACA GAGACACTCA GCTGGAGTTC CGAATACTCT 
GAAATGCAAC AGTTATCGAC CTCCAACTCT TCAGATACTG AAAGCAACAG GTGCAAACTC 
AGCTCTGGCT TGCTCCCCAA GCTGGCTATT TCGACAGATG GGGAACAAGA TGAGGCTGTC 
CCTTGCTCTG GAGACCCCAG AGAGGAGCCA GAGAAACCTG TCCCTCCCTC TGAGGAGTGT 
ACTCAGGAGG AGCCCGAGGT CACCACCCCA GCCAGCACCA TCAGCAGTTC CACACTGTCA 
GTTGGCAGTT TTTCAGAGCA CTTGGATCAG ATAAATGGGC GAAGCGAGTG TGTGGACAGT 
ACAGATAATT CCTCAAAGCC ATCCAGTGAA CCCACTTCTC ACGTGGCTCG ACAGCGCTTA 
GAAAGCACAG AGAAAAAGAA AATTTCTGGG AAAGTCACAA AGTCCCTCTC GGCCAGTGCT 
CTGTCCCTCA TGATCCCAGG AGATATGTTC GCTGTATCTC CATTGGGAAG CCCAATGTCC 
CCACACTCCC TGTCTTCAGA CCCTTCTTCT TCACGGGATT CCTCTCCCAG CCGAGACTCT 
TCTGCAGCAT CTGCCAGTCC GCATCAGCCC ATTGTCATCC ACAGCTCAGG CAAGAACTAT 
GGGTTCACCA TCCGTGCTAT CCGCGTGTAC GTGGGGGACA GTGACATCTA CACAGTGCAC 
CATATCGTCT GGAACGTAGA AGAAGGAAGT CCCGCATACC AGGCAGGACT GAAGGCCGGG 
GATCTGATCA CACACATCAA CGGAGAGCCG GTGCACGGCC TCGTCCACAC GGAAGTTATC 
GAGCTCCTGC TGAAGAGTGG GAATAAGGTG TCTATCACCA CTACTCCATT TGAAAACACA 
TCAATCAAAA CGGGACCAGC CAGGAGAAAC AGTTACAAGG GCCGGATGGT GAGACGAAGC 
AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC 
AAGCAGCCTT CTCCTTTGCT CCACACCAGC CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG 
TCATCTGGAG AGAGCCTCCC GGGTTCCCCA ACTCACAGCT TGTCCCCGAG GTCTCCAACA 
CCCAGTTATC GTTCTACTCC CGATTTTCCG TCAGGTACAA ATTCCTCCCA GAGCAGCTCC 
CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA GGTTCAGGGC ACATCCGGCC CAGCACCCTC 
CATGGCCTGG CTCCCAAACT CAGCGGGCAG CGATACCGCT CTGGAAGACG GAAGTCGGCT 
GGCAGCATCC CTCTCTCCCC GCTGGCCAGG ACACCCTCTC CCACTCCACA GCCTACCTCT 
CCTCAGCGTT CACCATCCCC ACTGTTGGGA CACTCACTGG GCAATGCCAA GATCACTCAG 
GCCTTTCCTA GCAAGATGCA CTCTCCCCCC ACAATCGTCA GACACATCGT GAGGCCCAAG 
AGTGCAGAGC CGCCCCGCTC CCCACTGCTG AAACGGGTGC AGTCGGAGGA AAAGTTGTCA 
CCCTCCTATG GCAGTGACAA GAAGCTTCTG TGCTCCCGCA AGCATAGCCT AGAGGTGACA 
CAAGAGGAGG TACAGAGGGA GCAGTGTCAG CGGGAAGTGA CACTGCAGAG CCTGGAAGAG 
AATGTGTGTG ACGCTCCTTC CCTCAGTCGG GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA 
CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA GAGTCTGTGG ATGACCTGGA CCGGGACAAG 
CTGAAAGCCA AGGTTGTCGT AAAGAAACCA GAAGAGAAAC ATGAATCGCA CCAGAAACCT 
CACAGCCTTG GTGGTGATTC GGAAAGCTAT GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA 
AAAGTGTACT CCAAGGGGTT GGAAAGGTCA GGCCATTTTG AAAACACATC AGCAGAGTTG 
CCTTCTGTGG GCAGCCTGCT GAAGGACACT CTTCACAAGC AGGCCAGCGT GAGGGCCAGC 
GAGGGGGTGA CCTCAGACGG GGCAGCTTGC AGCCTGACAC CAGGGGAGCA CAGCCAGTCT 
CTAGGTGACT TTAAGCGGGC CTCAGCTTCT GGCATTCTCC ATGATAGTGT GTGCCCCATC 
TCTGATAGGC CTGCTCCTGG AAAGGTTGAA TACTCGGAGA AGGCCTCTCA GGCCAAAGAG 
CTCCTTCGAA GTGAAAAACT AGACAGCAAG CTGGCCAATA TTGATTACCT CAGAAAGAAA 
ATGTCATTGG ATGACAAAGA TGACAGCCAC TGTGCCATCC TGAAACCCAA GATAACATCT 
AGCGCCCATG AATGTCTGCC AGGGAACCCC ATACGGCCCA TGGCAGGGCA ACAAGAGACC 
CCGCCAGCCT CTGAAAACCG GGCATTCATC AACAGTACCC ACACACCTCA GATGAGTGCA 
GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT GGCCGGGTAG AGAACGGAGG GGAGAAAGCA 
GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG AAGAGCCCCT CCGAGTATAA GCTAGAGGGC 
AGGTCAGTTT CATGTCTCAA GCCGATCGAG GGCACACTGG ACATTGCTCT CCTGTCTGGA 
CCTCACGCCT CCAAAACAGA GTTGCTTTCC CCAGAGCCTG CACAGAGTCC CAGCCCAGGC 
ATCAACGTGG GACCATGTGT GCCACTAGCT CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC 
TCCACCAGCC TGAGAGAGCC TTCCTCAGCC AACTTAAAAG TAAATAAATC TTATCTGCTG 
GAGCCTCGGT TCCTACCCCC GAGCCGGGCT CTCCAGGACT CTCTCGCAGC CTCTGGGCCA 
GAACCGAAGT CAAAGCCGGA AAGGAAGCTC ATTCATCCTT CTGCCAGGAG CCCAGCAACT 
GTCACAGAGA GCAATCTTCA GCAGAAAGAG GGTGGTCCCG CCACACACCA AGACCGCTCC 
ACTGACACCA GGAACCTCCC TGGCCCAGGG CAGACCCTAC ACAATGTGGA CCTACCCAGG 
CTGTGTACAC GTGCCCCACT CCCACCGGAA GGGACGCCCG CAAAGGAGAA GCCATGTCTG 
AAGGAACCCT CTGCCAAGGT GAAAAGCGAG TGGTCTGCCG TGAGGGATGA CGGACACAGA 
GATCCCTGTG CGAAGCTGTG CCCGGCAGAG ACTGGTAAAG CCAGCGACAG TTCCAAACCC 
CTGCCTTCCG GGGGGAGGAC CCAACCCGAT TTCTACAAGC AGACCCAGAC TTCGGAGAAA 
GCATGGGCGC ATGCAAAAAC AAACCACAAA GATAGCCAAG ATGAGGTGAA GTCCCTGGCC 
AGGGAGGACT CAGCTTCACT TTTATATGAA AAGGAGATAG GCCGGGCACG AAAAGGTCCT 
GAACCCAAAC CGGAAGTTCC TGCTACCCGG TGCCCTCCTC AGCCACCAGG AATTGAGGGT 
GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC TCTTTGCAGA AACAGGCTCC CAAAGAGCCA 
GACAGGAAGG AACAGACTTC GCAAAGGCCT GGAGGTAGTG GCCCTCAACA ACCCCCACCC 
ACCAAAGAGC TGTCTAACTC AGCATCCTGG CAGCACGGCA GTTCTCCGAG TCACACTTTA 
AAGAAGGAGC CCGGGACCAA AGCTGCCGCT GCAGAACCAA GCACCAGCCT TCATGACACT 
CCCCGATCTG CTACAGCCAC CACCACTGCC ATTGCCACCA CCACCACTAC CACCAGTGCC 
GGGCACAGTG ACTGCAGTAG CCATAAGGCC CGGCCTGGCC CTGACCCCAG CCCTTCAAAG 
TCTAAGCACC AAGACAGGTC CCTCTCCTCA CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC 
AAAGAGCCTG TCACTCAACC CCTGGGTGGT TCCATCAGAG AAGGCAAGGG TGGCAGCAAG 
GGTCCAGTGG ACACATTTTC TGCTGTCCTG ACCACCCAGG GCAAAGCAAG TGATGTGCTT 
GTGCAGGGAG AAGGTCGGGT CTCAATCATT GTCCACACTG AAGAGTGTCC TCTCGATGCC 
AAACTGAAAA ACACCAATGG AGGGTGTCCC CCAGAGATGC AGGCGAAGCA TCCACCCAGA 
CAAGGACATC TCAGTGAAGC AGCAGACCAG AAGCCACTCA TTGCTGGTGA GAAGCAAAGC 
CCGTCTCCAA AGCATCCCAA ACCATCCACT GTGAAAGATT ACCCCAGTCT GTGCAGACAG 
ACAGACAGAA GCCCAAGCCA TCAGGCTACC ACTGGGGACA GGAAGGCAGA AGGAAAGAAA 
TGCACAGACG CACTTTATGT CGCAGCCCCA GAGGGCTACA AGCCAGAGGC CAGCCCTTCT 
CTCCACCACG GCGAGACCGG ACTCAGAGGC TCAGAGAGGC CACCCATGGG CATGGGGAAG 
GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA GGTCCCCAGA AGTCACTGGC TGAAACAGGC 
AAGCCCAGCG GTATGAAAAG GTCACCCTCT GCCACCGTGC AGAGCTCTCT CCGCTCAGCT 
GCCCCCCCAG AAAAGTCTCT GAGTTACTCA GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG 
CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC TCATCTGCCA AGGCTACAGG GGGGACCTCA 
GAGTTCCCAG CCCCCAGCAG CAGGGACCAC AGGAAGCTTC AGTCTGGAGG AGACGGCCGA 
AGCCAAATGA TAAAGAGTGA CTCTCTGCCC TCCTTCCGCC TCTCCACCTC TGCTCTGGAG 
TCACATTTCC AGGATCCACA GGTGCCCATC GCATCAGGCC ACCGAGGCAG GGCACTGTCA 
GTAACTGCTG CCACAGGAGA ACCCAAAGGG AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG 
AAACAGAATG CGTGCAGAGA GGCGACCAGA GCACCCCCAG CCCCAAGCAC AGATCGCTCC 
CTCCCTCTTT CCTCAGAGAA AGACTTCGTG GTTCGGCAGA GAAGGGGCAA GGAGACCTTA 
AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_006517704.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517767.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X12, mRNA.

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CloneID	OMu49080
Clone ID Related Accession (Same CDS sequence)	XM_006517704.4 , XM_006517704.3
Accession Version	XM_006517704.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGA AGGAGCTGAG CCTCCCCCGA 
AGAGGAAGTC TTTGCCGAAC AAGCAACCGG AAGAGTTTGA TAGGCAATGG CCAGTCTCCA 
GCACTGCCTC GACCACACTC ACCTCTCTCT GCTCATGCAG GAAATAGCCC TCAAGACAGT 
CCAAGGAATT TCTCCCCCAG TGCCTCAGCC CATTTCTCAT TTGCGAGGAG AAATGACAGA 
ACGGATGGGC GCCGCTGGTC TCTGGCTTCT CTCCCTTCCT CAGGCTATGG GACAAATACC 
CCAAGCTCCA CCGTCTCCTC ATCCTGTTCT TCCCAGGAGA AGTTGCACCA GCTACCATAC 
CAGCCAACCC CAGATGAATT ACACTTCTTA TCCAAACACT TCTGCACAAC AGAAAGTATC 
GCCACTGAGA ACCGGTGCAG AAACACACCC ATGCGTCCAC GTTCCCGGAG TCTCAGCCCT 
GGACGGTCCC CCGCCTGCTG TGACCATGAA ATAATTATGA TGAACCATGT CTACAAAGAA 
AGGTTCCCAA AGGCCACAGC TCAGATGGAA GAGCGTCTGA AGGAGATCAT CACCAGCTAC 
TCTCCAGACC ATGTTCTCCC CTTGGCAGAT GGGGTACTTA GTTTCACTCA CCATCAGATC 
ATTGAGCTGG CTCGGGACTG TTTGGATAAA TCTCACCAGG GTCTCATCAC GTCGAGATAC 
TTTTTTGAGT TGCAGCACAA ACTGGACAAG TTGCTCCAGG AGGCTCACGA TCGCTCTGAA 
AGTGGAGAAC TGGCATTTAT CAAGCAACTA GTCCGAAAGA TCCTAATTGT CATTGCCCGC 
CCCGCTCGGT TATTGGAGTG TTTGGAATTT GATCCTGAAG AATTTTATTA CCTATTGGAA 
GCTGCAGAAG GCCATGCCAA AGAAGGCCAA GGAATCAAAA CTGACATCCC TAGGTATATC 
ATCAGCCAGC TGGGACTCAA TAAGGACCCT TTAGAAGAAA TGGCTCAGTT GGGGAATTAC 
GACAGTAGGA CAGCAGAGAC ACCAGAGATG GATGAGTCAG TGAGTAGCTC AAATACTTCC 
CTGAGACTTC GAAGGAAACC CCGAGAGAGT GATTTTGAAA CAATTAAATT GATCAGCAAT 
GGAGCCTACG GGGCAGTCTA CTTTGTTCGG CACAAAGAGT CCCGCCAGAG GTTTGCCATG 
AAGAAGATCA ACAAGCAGAA CCTCATCCTT CGGAACCAGA TCCAGCAGGC CTTCGTGGAG 
CGAGACATCC TGACTTTCGC AGAGAACCCC TTTGTGGTCA GCATGTATTG CTCCTTTGAA 
ACGAGGCGTC ACTTATGCAT GGTCATGGAG TATGTAGAAG GGGGAGACTG TGCGACCCTA 
ATGAAAAACA TGGGACCTCT CCCAGTTGAT ATGGCCAGAA TGTATTTCGC CGAGACCGTC 
TTGGCCTTGG AGTACCTGCA TAATTACGGA ATCGTACACA GGGACTTGAA GCCAGACAAC 
CTGTTGGTCA CCTCCATGGG GCACATAAAA CTGACTGACT TCGGCTTGTC TAAGGTGGGA 
TTAATGAGCA TGACCACCAA TCTCTATGAA GGCCACATAG AGAAGGACGC TCGAGAGTTC 
TTAGATAAAC AGGTCTGTGG TACACCTGAG TACATTGCCC CCGAGGTGAT TCTGAGACAG 
GGCTACGGGA AACCCGTGGA CTGGTGGGCC ATGGGCATTA TCCTCTATGA ATTTCTGGTC 
GGATGTGTGC CTTTCTTTGG GGACACTCCA GAAGAGCTAT TTGGACAAGT CATCAGTGAT 
GAAATCAACT GGCCTGAAAA GGACGAGGCC CCTCCTCCAG ACGCTCAGGA GCTGATTACC 
TTGCTCCTCA GGCAGAATCC GCTGGAGAGG CTGGGAACAG GTGGAGCCTA TGAAGTGAAG 
CAGCATCGTT TCTTCCGCTC CTTAGACTGG AACAGTTTGC TGAGACAGAA GGCGGAATTT 
ATTCCCCAAC TGGAATCGGA GGATGACACA AGTTATTTTG ATACTCGGTC AGAGAAGTAT 
CATCACATGG AGACGGAGGA GGAGGACGAT ACAAACGATG AGGACTTCAC CGTGGAGATA 
AGGCAGTTTT CTTCCTGTTC ACACAGGTTT TCAAAAGTTT TCAGCAGTAT AGATCGCATA 
ACTCAAAATT CAGGAGAAGA CAAAGATGAC TCTGAGGACA AGACCAAAAG CACAACGTTG 
CCATCCACAG AGACACTCAG CTGGAGTTCC GAATACTCTG AAATGCAACA GTTATCGACC 
TCCAACTCTT CAGATACTGA AAGCAACAGG TGCAAACTCA GCTCTGGCTT GCTCCCCAAG 
CTGGCTATTT CGACAGATGG GGAACAAGAT GAGGCTGTCC CTTGCTCTGG AGACCCCAGA 
GAGGAGCCAG AGAAACCTGT CCCTCCCTCT GAGGAGTGTA CTCAGGAGGA GCCCGAGGTC 
ACCACCCCAG CCAGCACCAT CAGCAGTTCC ACACTGTCAG TTGGCAGTTT TTCAGAGCAC 
TTGGATCAGA TAAATGGGCG AAGCGAGTGT GTGGACAGTA CAGATAATTC CTCAAAGCCA 
TCCAGTGAAC CCACTTCTCA CGTGGCTCGA CAGCGCTTAG AAAGCACAGA GAAAAAGAAA 
ATTTCTGGGA AAGTCACAAA GTCCCTCTCG GCCAGTGCTC TGTCCCTCAT GATCCCAGGA 
GATATGTTCG CTGTATCTCC ATTGGGAAGC CCAATGTCCC CACACTCCCT GTCTTCAGAC 
CCTTCTTCTT CACGGGATTC CTCTCCCAGC CGAGACTCTT CTGCAGCATC TGCCAGTCCG 
CATCAGCCCA TTGTCATCCA CAGCTCAGGC AAGAACTATG GGTTCACCAT CCGTGCTATC 
CGCGTGTACG TGGGGGACAG TGACATCTAC ACAGTGCACC ATATCGTCTG GAACGTAGAA 
GAAGGAAGTC CCGCATACCA GGCAGGACTG AAGGCCGGGG ATCTGATCAC ACACATCAAC 
GGAGAGCCGG TGCACGGCCT CGTCCACACG GAAGTTATCG AGCTCCTGCT GAAGAGTGGG 
AATAAGGTGT CTATCACCAC TACTCCATTT GAAAACACAT CAATCAAAAC GGGACCAGCC 
AGGAGAAACA GTTACAAGGG CCGGATGGTG AGACGAAGCA AGAAGTCCAA GAAGAAGGAG 
AGTCTAGAAA GGAGGAGATC TCTCTTCAAG AAGCTGGCCA AGCAGCCTTC TCCTTTGCTC 
CACACCAGCC GAAGTTTCTC CTGCTTAAAC CGGTCCCTGT CATCTGGAGA GAGCCTCCCG 
GGTTCCCCAA CTCACAGCTT GTCCCCGAGG TCTCCAACAC CCAGTTATCG TTCTACTCCC 
GATTTTCCGT CAGGTACAAA TTCCTCCCAG AGCAGCTCCC CAAGTTCAAG TGCCCCCAAT 
TCTCCAGCAG GTTCAGGGCA CATCCGGCCC AGCACCCTCC ATGGCCTGGC TCCCAAACTC 
AGCGGGCAGC GATACCGCTC TGGAAGACGG AAGTCGGCTG GCAGCATCCC TCTCTCCCCG 
CTGGCCAGGA CACCCTCTCC CACTCCACAG CCTACCTCTC CTCAGCGTTC ACCATCCCCA 
CTGTTGGGAC ACTCACTGGG CAATGCCAAG ATCACTCAGG CCTTTCCTAG CAAGATGCAC 
TCTCCCCCCA CAATCGTCAG ACACATCGTG AGGCCCAAGA GTGCAGAGCC GCCCCGCTCC 
CCACTGCTGA AACGGGTGCA GTCGGAGGAA AAGTTGTCAC CCTCCTATGG CAGTGACAAG 
AAGCTTCTGT GCTCCCGCAA GCATAGCCTA GAGGTGACAC AAGAGGAGGT ACAGAGGGAG 
CAGTGTCAGC GGGAAGTGAC ACTGCAGAGC CTGGAAGAGA ATGTGTGTGA CGCTCCTTCC 
CTCAGTCGGG CCAGGCCAGT GGAGCAAGGC TGTCTGAAAC GCCCCGTGTC CCGGAAGGTG 
GGCAGGCAAG AGTCTGTGGA TGACCTGGAC CGGGACAAGC TGAAAGCCAA GGTTGTCGTA 
AAGAAACCAG AAGAGAAACA TGAATCGCAC CAGAAACCTC ACAGCCTTGG TGGTGATTCG 
GAAAGCTATG CTCTCTTCAG GCTAGAGGAG AGAGAGAAAA AAGTGTACTC CAAGGGGTTG 
GAAAGGTCAG GCCATTTTGA AAACACATCA GCAGAGTTGC CTTCTGTGGG CAGCCTGCTG 
AAGGACACTC TTCACAAGCA GGCCAGCGTG AGGGCCAGCG AGGGGGTGAC CTCAGACGGG 
GCAGCTTGCA GCCTGACACC AGGGGAGCAC AGCCAGTCTC TAGGTGACTT TAAGCGGGCC 
TCAGCTTCTG GCATTCTCCA TGATAGTGTG TGCCCCATCT CTGATAGGCC TGCTCCTGGA 
AAGGTTGAAT ACTCGGAGAA GGCCTCTCAG GCCAAAGAGC TCCTTCGAAG TGAAAAACTA 
GACAGCAAGC TGGCCAATAT TGATTACCTC AGAAAGAAAA TGTCATTGGA TGACAAAGAT 
GACAGCCACT GTGCCATCCT GAAACCCAAG ATAACATCTA GCGCCCATGA ATGTCTGCCA 
GGGAACCCCA TACGGCCCAT GGCAGGGCAA CAAGAGACCC CGCCAGCCTC TGAAAACCGG 
GCATTCATCA ACAGTACCCA CACACCTCAG ATGAGTGCAG TTTCCTTTGT TCCTCTCAAA 
GCCTTAGCTG GCCGGGTAGA GAACGGAGGG GAGAAAGCAG GCTTAGCTGC TCCCGAGTCC 
CCTGTCAGGA AGAGCCCCTC CGAGTATAAG CTAGAGGGCA GGTCAGTTTC ATGTCTCAAG 
CCGATCGAGG GCACACTGGA CATTGCTCTC CTGTCTGGAC CTCACGCCTC CAAAACAGAG 
TTGCTTTCCC CAGAGCCTGC ACAGAGTCCC AGCCCAGGCA TCAACGTGGG ACCATGTGTG 
CCACTAGCTC TTCCTGGGAG CAGTGGGAAA AAGGGAGACT CCACCAGCCT GAGAGAGCCT 
TCCTCAGCCA ACTTAAAAGT AAATAAATCT TATCTGCTGG AGCCTCGGTT CCTACCCCCG 
AGCCGGGCTC TCCAGGACTC TCTCGCAGCC TCTGGGCCAG AACCGAAGTC AAAGCCGGAA 
AGGAAGCTCA TTCATCCTTC TGCCAGGAGC CCAGCAACTG TCACAGAGAG CAATCTTCAG 
CAGAAAGAGG GTGGTCCCGC CACACACCAA GACCGCTCCA CTGACACCAG GAACCTCCCT 
GGCCCAGGGC AGACCCTACA CAATGTGGAC CTACCCAGGC TGTGTACACG TGCCCCACTC 
CCACCGGAAG GGACGCCCGC AAAGGAGAAG CCATGTCTGA AGGAACCCTC TGCCAAGGTG 
AAAAGCGAGT GGTCTGCCGT GAGGGATGAC GGACACAGAG ATCCCTGTGC GAAGCTGTGC 
CCGGCAGAGA CTGGTAAAGC CAGCGACAGT TCCAAACCCC TGCCTTCCGG GGGGAGGACC 
CAACCCGATT TCTACAAGCA GACCCAGACT TCGGAGAAAG CATGGGCGCA TGCAAAAACA 
AACCACAAAG ATAGCCAAGA TGAGGTGAAG TCCCTGGCCA GGGAGGACTC AGCTTCACTT 
TTATATGAAA AGGAGATAGG CCGGGCACGA AAAGGTCCTG AACCCAAACC GGAAGTTCCT 
GCTACCCGGT GCCCTCCTCA GCCACCAGGA ATTGAGGGTG AGAAGCGAGA AAAGCTCTCC 
GCTGCCCCCT CTTTGCAGAA ACAGGCTCCC AAAGAGCCAG ACAGGAAGGA ACAGACTTCG 
CAAAGGCCTG GAGGTAGTGG CCCTCAACAA CCCCCACCCA CCAAAGAGCT GTCTAACTCA 
GCATCCTGGC AGCACGGCAG TTCTCCGAGT CACACTTTAA AGAAGGAGCC CGGGACCAAA 
GCTGCCGCTG CAGAACCAAG CACCAGCCTT CATGACACTC CCCGATCTGC TACAGCCACC 
ACCACTGCCA TTGCCACCAC CACCACTACC ACCAGTGCCG GGCACAGTGA CTGCAGTAGC 
CATAAGGCCC GGCCTGGCCC TGACCCCAGC CCTTCAAAGT CTAAGCACCA AGACAGGTCC 
CTCTCCTCAC AGAAGCTGAG TGCTGGCTCT GCAAAAGGCA AAGAGCCTGT CACTCAACCC 
CTGGGTGGTT CCATCAGAGA AGGCAAGGGT GGCAGCAAGG GTCCAGTGGA CACATTTTCT 
GCTGTCCTGA CCACCCAGGG CAAAGCAAGT GATGTGCTTG TGCAGGGAGA AGGTCGGGTC 
TCAATCATTG TCCACACTGA AGAGTGTCCT CTCGATGCCA AACTGAAAAA CACCAATGGA 
GGGTGTCCCC CAGAGATGCA GGCGAAGCAT CCACCCAGAC AAGGACATCT CAGTGAAGCA 
GCAGACCAGA AGCCACTCAT TGCTGGTGAG AAGCAAAGCC CGTCTCCAAA GCATCCCAAA 
CCATCCACTG TGAAAGATTA CCCCAGTCTG TGCAGACAGA CAGACAGAAG CCCAAGCCAT 
CAGGCTACCA CTGGGGACAG GAAGGCAGAA GGAAAGAAAT GCACAGACGC ACTTTATGTC 
GCAGCCCCAG AGGGCTACAA GCCAGAGGCC AGCCCTTCTC TCCACCACGG CGAGACCGGA 
CTCAGAGGCT CAGAGAGGCC ACCCATGGGC ATGGGGAAGG GCTTCTCTGA GCCCAAGGGG 
AAAGGGCCAG GTCCCCAGAA GTCACTGGCT GAAACAGGCA AGCCCAGCGG TATGAAAAGG 
TCACCCTCTG CCACCGTGCA GAGCTCTCTC CGCTCAGCTG CCCCCCCAGA AAAGTCTCTG 
AGTTACTCAG CCAGCTTTCC CGAGGCCCAG CCTGGAGTGC GAGAGGTCCC TGCAGCCAAC 
AGCAGCCCCT CATCTGCCAA GGCTACAGGG GGGACCTCAG AGTTCCCAGC CCCCAGCAGC 
AGGGACCACA GGAAGCTTCA GTCTGGAGGA GACGGCCGAA GCCAAATGAT AAAGAGTGAC 
TCTCTGCCCT CCTTCCGCCT CTCCACCTCT GCTCTGGAGT CACATTTCCA GGATCCACAG 
GTGCCCATCG CATCAGGCCA CCGAGGCAGG GCACTGTCAG TAACTGCTGC CACAGGAGAA 
CCCAAAGGGA GAGAGCTCGC CCAGCCTCCC CCAGTCAGGA AACAGAATGC GTGCAGAGAG 
GCGACCAGAG CACCCCCAGC CCCAAGCACA GATCGCTCCC TCCCTCTTTC CTCAGAGAAA 
GACTTCGTGG TTCGGCAGAG AAGGGGCAAG GAGACCTTAA GGAGCAGTCC TCACAAAAAG 
GCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517767.1
CDS	776..8044
Translation	MDESSILRRRGLQKELSLPRRGSLCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_006517704.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517704.3

ORF Insert Sequence:

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGA AGGAGCTGAG CCTCCCCCGA 
AGAGGAAGTC TTTGCCGAAC AAGCAACCGG AAGAGTTTGA TAGGCAATGG CCAGTCTCCA 
GCACTGCCTC GACCACACTC ACCTCTCTCT GCTCATGCAG GAAATAGCCC TCAAGACAGT 
CCAAGGAATT TCTCCCCCAG TGCCTCAGCC CATTTCTCAT TTGCGAGGAG AAATGACAGA 
ACGGATGGGC GCCGCTGGTC TCTGGCTTCT CTCCCTTCCT CAGGCTATGG GACAAATACC 
CCAAGCTCCA CCGTCTCCTC ATCCTGTTCT TCCCAGGAGA AGTTGCACCA GCTACCATAC 
CAGCCAACCC CAGATGAATT ACACTTCTTA TCCAAACACT TCTGCACAAC AGAAAGTATC 
GCCACTGAGA ACCGGTGCAG AAACACACCC ATGCGTCCAC GTTCCCGGAG TCTCAGCCCT 
GGACGGTCCC CCGCCTGCTG TGACCATGAA ATAATTATGA TGAACCATGT CTACAAAGAA 
AGGTTCCCAA AGGCCACAGC TCAGATGGAA GAGCGTCTGA AGGAGATCAT CACCAGCTAC 
TCTCCAGACC ATGTTCTCCC CTTGGCAGAT GGGGTACTTA GTTTCACTCA CCATCAGATC 
ATTGAGCTGG CTCGGGACTG TTTGGATAAA TCTCACCAGG GTCTCATCAC GTCGAGATAC 
TTTTTTGAGT TGCAGCACAA ACTGGACAAG TTGCTCCAGG AGGCTCACGA TCGCTCTGAA 
AGTGGAGAAC TGGCATTTAT CAAGCAACTA GTCCGAAAGA TCCTAATTGT CATTGCCCGC 
CCCGCTCGGT TATTGGAGTG TTTGGAATTT GATCCTGAAG AATTTTATTA CCTATTGGAA 
GCTGCAGAAG GCCATGCCAA AGAAGGCCAA GGAATCAAAA CTGACATCCC TAGGTATATC 
ATCAGCCAGC TGGGACTCAA TAAGGACCCT TTAGAAGAAA TGGCTCAGTT GGGGAATTAC 
GACAGTAGGA CAGCAGAGAC ACCAGAGATG GATGAGTCAG TGAGTAGCTC AAATACTTCC 
CTGAGACTTC GAAGGAAACC CCGAGAGAGT GATTTTGAAA CAATTAAATT GATCAGCAAT 
GGAGCCTACG GGGCAGTCTA CTTTGTTCGG CACAAAGAGT CCCGCCAGAG GTTTGCCATG 
AAGAAGATCA ACAAGCAGAA CCTCATCCTT CGGAACCAGA TCCAGCAGGC CTTCGTGGAG 
CGAGACATCC TGACTTTCGC AGAGAACCCC TTTGTGGTCA GCATGTATTG CTCCTTTGAA 
ACGAGGCGTC ACTTATGCAT GGTCATGGAG TATGTAGAAG GGGGAGACTG TGCGACCCTA 
ATGAAAAACA TGGGACCTCT CCCAGTTGAT ATGGCCAGAA TGTATTTCGC CGAGACCGTC 
TTGGCCTTGG AGTACCTGCA TAATTACGGA ATCGTACACA GGGACTTGAA GCCAGACAAC 
CTGTTGGTCA CCTCCATGGG GCACATAAAA CTGACTGACT TCGGCTTGTC TAAGGTGGGA 
TTAATGAGCA TGACCACCAA TCTCTATGAA GGCCACATAG AGAAGGACGC TCGAGAGTTC 
TTAGATAAAC AGGTCTGTGG TACACCTGAG TACATTGCCC CCGAGGTGAT TCTGAGACAG 
GGCTACGGGA AACCCGTGGA CTGGTGGGCC ATGGGCATTA TCCTCTATGA ATTTCTGGTC 
GGATGTGTGC CTTTCTTTGG GGACACTCCA GAAGAGCTAT TTGGACAAGT CATCAGTGAT 
GAAATCAACT GGCCTGAAAA GGACGAGGCC CCTCCTCCAG ACGCTCAGGA GCTGATTACC 
TTGCTCCTCA GGCAGAATCC GCTGGAGAGG CTGGGAACAG GTGGAGCCTA TGAAGTGAAG 
CAGCATCGTT TCTTCCGCTC CTTAGACTGG AACAGTTTGC TGAGACAGAA GGCGGAATTT 
ATTCCCCAAC TGGAATCGGA GGATGACACA AGTTATTTTG ATACTCGGTC AGAGAAGTAT 
CATCACATGG AGACGGAGGA GGAGGACGAT ACAAACGATG AGGACTTCAC CGTGGAGATA 
AGGCAGTTTT CTTCCTGTTC ACACAGGTTT TCAAAAGTTT TCAGCAGTAT AGATCGCATA 
ACTCAAAATT CAGGAGAAGA CAAAGATGAC TCTGAGGACA AGACCAAAAG CACAACGTTG 
CCATCCACAG AGACACTCAG CTGGAGTTCC GAATACTCTG AAATGCAACA GTTATCGACC 
TCCAACTCTT CAGATACTGA AAGCAACAGG TGCAAACTCA GCTCTGGCTT GCTCCCCAAG 
CTGGCTATTT CGACAGATGG GGAACAAGAT GAGGCTGTCC CTTGCTCTGG AGACCCCAGA 
GAGGAGCCAG AGAAACCTGT CCCTCCCTCT GAGGAGTGTA CTCAGGAGGA GCCCGAGGTC 
ACCACCCCAG CCAGCACCAT CAGCAGTTCC ACACTGTCAG TTGGCAGTTT TTCAGAGCAC 
TTGGATCAGA TAAATGGGCG AAGCGAGTGT GTGGACAGTA CAGATAATTC CTCAAAGCCA 
TCCAGTGAAC CCACTTCTCA CGTGGCTCGA CAGCGCTTAG AAAGCACAGA GAAAAAGAAA 
ATTTCTGGGA AAGTCACAAA GTCCCTCTCG GCCAGTGCTC TGTCCCTCAT GATCCCAGGA 
GATATGTTCG CTGTATCTCC ATTGGGAAGC CCAATGTCCC CACACTCCCT GTCTTCAGAC 
CCTTCTTCTT CACGGGATTC CTCTCCCAGC CGAGACTCTT CTGCAGCATC TGCCAGTCCG 
CATCAGCCCA TTGTCATCCA CAGCTCAGGC AAGAACTATG GGTTCACCAT CCGTGCTATC 
CGCGTGTACG TGGGGGACAG TGACATCTAC ACAGTGCACC ATATCGTCTG GAACGTAGAA 
GAAGGAAGTC CCGCATACCA GGCAGGACTG AAGGCCGGGG ATCTGATCAC ACACATCAAC 
GGAGAGCCGG TGCACGGCCT CGTCCACACG GAAGTTATCG AGCTCCTGCT GAAGAGTGGG 
AATAAGGTGT CTATCACCAC TACTCCATTT GAAAACACAT CAATCAAAAC GGGACCAGCC 
AGGAGAAACA GTTACAAGGG CCGGATGGTG AGACGAAGCA AGAAGTCCAA GAAGAAGGAG 
AGTCTAGAAA GGAGGAGATC TCTCTTCAAG AAGCTGGCCA AGCAGCCTTC TCCTTTGCTC 
CACACCAGCC GAAGTTTCTC CTGCTTAAAC CGGTCCCTGT CATCTGGAGA GAGCCTCCCG 
GGTTCCCCAA CTCACAGCTT GTCCCCGAGG TCTCCAACAC CCAGTTATCG TTCTACTCCC 
GATTTTCCGT CAGGTACAAA TTCCTCCCAG AGCAGCTCCC CAAGTTCAAG TGCCCCCAAT 
TCTCCAGCAG GTTCAGGGCA CATCCGGCCC AGCACCCTCC ATGGCCTGGC TCCCAAACTC 
AGCGGGCAGC GATACCGCTC TGGAAGACGG AAGTCGGCTG GCAGCATCCC TCTCTCCCCG 
CTGGCCAGGA CACCCTCTCC CACTCCACAG CCTACCTCTC CTCAGCGTTC ACCATCCCCA 
CTGTTGGGAC ACTCACTGGG CAATGCCAAG ATCACTCAGG CCTTTCCTAG CAAGATGCAC 
TCTCCCCCCA CAATCGTCAG ACACATCGTG AGGCCCAAGA GTGCAGAGCC GCCCCGCTCC 
CCACTGCTGA AACGGGTGCA GTCGGAGGAA AAGTTGTCAC CCTCCTATGG CAGTGACAAG 
AAGCTTCTGT GCTCCCGCAA GCATAGCCTA GAGGTGACAC AAGAGGAGGT ACAGAGGGAG 
CAGTGTCAGC GGGAAGTGAC ACTGCAGAGC CTGGAAGAGA ATGTGTGTGA CGCTCCTTCC 
CTCAGTCGGG CCAGGCCAGT GGAGCAAGGC TGTCTGAAAC GCCCCGTGTC CCGGAAGGTG 
GGCAGGCAAG AGTCTGTGGA TGACCTGGAC CGGGACAAGC TGAAAGCCAA GGTTGTCGTA 
AAGAAACCAG AAGAGAAACA TGAATCGCAC CAGAAACCTC ACAGCCTTGG TGGTGATTCG 
GAAAGCTATG CTCTCTTCAG GCTAGAGGAG AGAGAGAAAA AAGTGTACTC CAAGGGGTTG 
GAAAGGTCAG GCCATTTTGA AAACACATCA GCAGAGTTGC CTTCTGTGGG CAGCCTGCTG 
AAGGACACTC TTCACAAGCA GGCCAGCGTG AGGGCCAGCG AGGGGGTGAC CTCAGACGGG 
GCAGCTTGCA GCCTGACACC AGGGGAGCAC AGCCAGTCTC TAGGTGACTT TAAGCGGGCC 
TCAGCTTCTG GCATTCTCCA TGATAGTGTG TGCCCCATCT CTGATAGGCC TGCTCCTGGA 
AAGGTTGAAT ACTCGGAGAA GGCCTCTCAG GCCAAAGAGC TCCTTCGAAG TGAAAAACTA 
GACAGCAAGC TGGCCAATAT TGATTACCTC AGAAAGAAAA TGTCATTGGA TGACAAAGAT 
GACAGCCACT GTGCCATCCT GAAACCCAAG ATAACATCTA GCGCCCATGA ATGTCTGCCA 
GGGAACCCCA TACGGCCCAT GGCAGGGCAA CAAGAGACCC CGCCAGCCTC TGAAAACCGG 
GCATTCATCA ACAGTACCCA CACACCTCAG ATGAGTGCAG TTTCCTTTGT TCCTCTCAAA 
GCCTTAGCTG GCCGGGTAGA GAACGGAGGG GAGAAAGCAG GCTTAGCTGC TCCCGAGTCC 
CCTGTCAGGA AGAGCCCCTC CGAGTATAAG CTAGAGGGCA GGTCAGTTTC ATGTCTCAAG 
CCGATCGAGG GCACACTGGA CATTGCTCTC CTGTCTGGAC CTCACGCCTC CAAAACAGAG 
TTGCTTTCCC CAGAGCCTGC ACAGAGTCCC AGCCCAGGCA TCAACGTGGG ACCATGTGTG 
CCACTAGCTC TTCCTGGGAG CAGTGGGAAA AAGGGAGACT CCACCAGCCT GAGAGAGCCT 
TCCTCAGCCA ACTTAAAAGT AAATAAATCT TATCTGCTGG AGCCTCGGTT CCTACCCCCG 
AGCCGGGCTC TCCAGGACTC TCTCGCAGCC TCTGGGCCAG AACCGAAGTC AAAGCCGGAA 
AGGAAGCTCA TTCATCCTTC TGCCAGGAGC CCAGCAACTG TCACAGAGAG CAATCTTCAG 
CAGAAAGAGG GTGGTCCCGC CACACACCAA GACCGCTCCA CTGACACCAG GAACCTCCCT 
GGCCCAGGGC AGACCCTACA CAATGTGGAC CTACCCAGGC TGTGTACACG TGCCCCACTC 
CCACCGGAAG GGACGCCCGC AAAGGAGAAG CCATGTCTGA AGGAACCCTC TGCCAAGGTG 
AAAAGCGAGT GGTCTGCCGT GAGGGATGAC GGACACAGAG ATCCCTGTGC GAAGCTGTGC 
CCGGCAGAGA CTGGTAAAGC CAGCGACAGT TCCAAACCCC TGCCTTCCGG GGGGAGGACC 
CAACCCGATT TCTACAAGCA GACCCAGACT TCGGAGAAAG CATGGGCGCA TGCAAAAACA 
AACCACAAAG ATAGCCAAGA TGAGGTGAAG TCCCTGGCCA GGGAGGACTC AGCTTCACTT 
TTATATGAAA AGGAGATAGG CCGGGCACGA AAAGGTCCTG AACCCAAACC GGAAGTTCCT 
GCTACCCGGT GCCCTCCTCA GCCACCAGGA ATTGAGGGTG AGAAGCGAGA AAAGCTCTCC 
GCTGCCCCCT CTTTGCAGAA ACAGGCTCCC AAAGAGCCAG ACAGGAAGGA ACAGACTTCG 
CAAAGGCCTG GAGGTAGTGG CCCTCAACAA CCCCCACCCA CCAAAGAGCT GTCTAACTCA 
GCATCCTGGC AGCACGGCAG TTCTCCGAGT CACACTTTAA AGAAGGAGCC CGGGACCAAA 
GCTGCCGCTG CAGAACCAAG CACCAGCCTT CATGACACTC CCCGATCTGC TACAGCCACC 
ACCACTGCCA TTGCCACCAC CACCACTACC ACCAGTGCCG GGCACAGTGA CTGCAGTAGC 
CATAAGGCCC GGCCTGGCCC TGACCCCAGC CCTTCAAAGT CTAAGCACCA AGACAGGTCC 
CTCTCCTCAC AGAAGCTGAG TGCTGGCTCT GCAAAAGGCA AAGAGCCTGT CACTCAACCC 
CTGGGTGGTT CCATCAGAGA AGGCAAGGGT GGCAGCAAGG GTCCAGTGGA CACATTTTCT 
GCTGTCCTGA CCACCCAGGG CAAAGCAAGT GATGTGCTTG TGCAGGGAGA AGGTCGGGTC 
TCAATCATTG TCCACACTGA AGAGTGTCCT CTCGATGCCA AACTGAAAAA CACCAATGGA 
GGGTGTCCCC CAGAGATGCA GGCGAAGCAT CCACCCAGAC AAGGACATCT CAGTGAAGCA 
GCAGACCAGA AGCCACTCAT TGCTGGTGAG AAGCAAAGCC CGTCTCCAAA GCATCCCAAA 
CCATCCACTG TGAAAGATTA CCCCAGTCTG TGCAGACAGA CAGACAGAAG CCCAAGCCAT 
CAGGCTACCA CTGGGGACAG GAAGGCAGAA GGAAAGAAAT GCACAGACGC ACTTTATGTC 
GCAGCCCCAG AGGGCTACAA GCCAGAGGCC AGCCCTTCTC TCCACCACGG CGAGACCGGA 
CTCAGAGGCT CAGAGAGGCC ACCCATGGGC ATGGGGAAGG GCTTCTCTGA GCCCAAGGGG 
AAAGGGCCAG GTCCCCAGAA GTCACTGGCT GAAACAGGCA AGCCCAGCGG TATGAAAAGG 
TCACCCTCTG CCACCGTGCA GAGCTCTCTC CGCTCAGCTG CCCCCCCAGA AAAGTCTCTG 
AGTTACTCAG CCAGCTTTCC CGAGGCCCAG CCTGGAGTGC GAGAGGTCCC TGCAGCCAAC 
AGCAGCCCCT CATCTGCCAA GGCTACAGGG GGGACCTCAG AGTTCCCAGC CCCCAGCAGC 
AGGGACCACA GGAAGCTTCA GTCTGGAGGA GACGGCCGAA GCCAAATGAT AAAGAGTGAC 
TCTCTGCCCT CCTTCCGCCT CTCCACCTCT GCTCTGGAGT CACATTTCCA GGATCCACAG 
GTGCCCATCG CATCAGGCCA CCGAGGCAGG GCACTGTCAG TAACTGCTGC CACAGGAGAA 
CCCAAAGGGA GAGAGCTCGC CCAGCCTCCC CCAGTCAGGA AACAGAATGC GTGCAGAGAG 
GCGACCAGAG CACCCCCAGC CCCAAGCACA GATCGCTCCC TCCCTCTTTC CTCAGAGAAA 
GACTTCGTGG TTCGGCAGAG AAGGGGCAAG GAGACCTTAA GGAGCAGTCC TCACAAAAAG 
GCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_006517705.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517768.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X13, mRNA.

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CloneID	OMu49081
Clone ID Related Accession (Same CDS sequence)	XM_006517705.4 , XM_006517705.3
Accession Version	XM_006517705.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517705.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGA AGGAGCTGAG CCTCCCCCGA 
AGAGGAAGTC TTTGCCGAAC AAGCAACCGG AAGAGTTTGA TAGGCAATGG CCAGTCTCCA 
GCACTGCCTC GACCACACTC ACCTCTCTCT GCTCATGCAG GAAATAGCCC TCAAGACAGT 
CCAAGGAATT TCTCCCCCAG TGCCTCAGCC CATTTCTCAT TTGCGAGGAG AACGGATGGG 
CGCCGCTGGT CTCTGGCTTC TCTCCCTTCC TCAGGCTATG GGACAAATAC CCCAAGCTCC 
ACCGTCTCCT CATCCTGTTC TTCCCAGGAG AAGTTGCACC AGCTACCATA CCAGCCAACC 
CCAGATGAAT TACACTTCTT ATCCAAACAC TTCTGCACAA CAGAAAGTAT CGCCACTGAG 
AACCGGTGCA GAAACACACC CATGCGTCCA CGTTCCCGGA GTCTCAGCCC TGGACGGTCC 
CCCGCCTGCT GTGACCATGA AATAATTATG ATGAACCATG TCTACAAAGA AAGGTTCCCA 
AAGGCCACAG CTCAGATGGA AGAGCGTCTG AAGGAGATCA TCACCAGCTA CTCTCCAGAC 
CATGTTCTCC CCTTGGCAGA TGGGGTACTT AGTTTCACTC ACCATCAGAT CATTGAGCTG 
GCTCGGGACT GTTTGGATAA ATCTCACCAG GGTCTCATCA CGTCGAGATA CTTTTTTGAG 
TTGCAGCACA AACTGGACAA GTTGCTCCAG GAGGCTCACG ATCGCTCTGA AAGTGGAGAA 
CTGGCATTTA TCAAGCAACT AGTCCGAAAG ATCCTAATTG TCATTGCCCG CCCCGCTCGG 
TTATTGGAGT GTTTGGAATT TGATCCTGAA GAATTTTATT ACCTATTGGA AGCTGCAGAA 
GGCCATGCCA AAGAAGGCCA AGGAATCAAA ACTGACATCC CTAGGTATAT CATCAGCCAG 
CTGGGACTCA ATAAGGACCC TTTAGAAGAA ATGGCTCAGT TGGGGAATTA CGACAGTAGG 
ACAGCAGAGA CACCAGAGAT GGATGAGTCA GTGAGTAGCT CAAATACTTC CCTGAGACTT 
CGAAGGAAAC CCCGAGAGAG TGATTTTGAA ACAATTAAAT TGATCAGCAA TGGAGCCTAC 
GGGGCAGTCT ACTTTGTTCG GCACAAAGAG TCCCGCCAGA GGTTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTCATCCT TCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGAGACATC 
CTGACTTTCG CAGAGAACCC CTTTGTGGTC AGCATGTATT GCTCCTTTGA AACGAGGCGT 
CACTTATGCA TGGTCATGGA GTATGTAGAA GGGGGAGACT GTGCGACCCT AATGAAAAAC 
ATGGGACCTC TCCCAGTTGA TATGGCCAGA ATGTATTTCG CCGAGACCGT CTTGGCCTTG 
GAGTACCTGC ATAATTACGG AATCGTACAC AGGGACTTGA AGCCAGACAA CCTGTTGGTC 
ACCTCCATGG GGCACATAAA ACTGACTGAC TTCGGCTTGT CTAAGGTGGG ATTAATGAGC 
ATGACCACCA ATCTCTATGA AGGCCACATA GAGAAGGACG CTCGAGAGTT CTTAGATAAA 
CAGGTCTGTG GTACACCTGA GTACATTGCC CCCGAGGTGA TTCTGAGACA GGGCTACGGG 
AAACCCGTGG ACTGGTGGGC CATGGGCATT ATCCTCTATG AATTTCTGGT CGGATGTGTG 
CCTTTCTTTG GGGACACTCC AGAAGAGCTA TTTGGACAAG TCATCAGTGA TGAAATCAAC 
TGGCCTGAAA AGGACGAGGC CCCTCCTCCA GACGCTCAGG AGCTGATTAC CTTGCTCCTC 
AGGCAGAATC CGCTGGAGAG GCTGGGAACA GGTGGAGCCT ATGAAGTGAA GCAGCATCGT 
TTCTTCCGCT CCTTAGACTG GAACAGTTTG CTGAGACAGA AGGCGGAATT TATTCCCCAA 
CTGGAATCGG AGGATGACAC AAGTTATTTT GATACTCGGT CAGAGAAGTA TCATCACATG 
GAGACGGAGG AGGAGGACGA TACAAACGAT GAGGACTTCA CCGTGGAGAT AAGGCAGTTT 
TCTTCCTGTT CACACAGGTT TTCAAAAGTT TTCAGCAGTA TAGATCGCAT AACTCAAAAT 
TCAGGAGAAG ACAAAGATGA CTCTGAGGAC AAGACCAAAA GCACAACGTT GCCATCCACA 
GAGACACTCA GCTGGAGTTC CGAATACTCT GAAATGCAAC AGTTATCGAC CTCCAACTCT 
TCAGATACTG AAAGCAACAG GTGCAAACTC AGCTCTGGCT TGCTCCCCAA GCTGGCTATT 
TCGACAGATG GGGAACAAGA TGAGGCTGTC CCTTGCTCTG GAGACCCCAG AGAGGAGCCA 
GAGAAACCTG TCCCTCCCTC TGAGGAGTGT ACTCAGGAGG AGCCCGAGGT CACCACCCCA 
GCCAGCACCA TCAGCAGTTC CACACTGTCA GTTGGCAGTT TTTCAGAGCA CTTGGATCAG 
ATAAATGGGC GAAGCGAGTG TGTGGACAGT ACAGATAATT CCTCAAAGCC ATCCAGTGAA 
CCCACTTCTC ACGTGGCTCG ACAGCGCTTA GAAAGCACAG AGAAAAAGAA AATTTCTGGG 
AAAGTCACAA AGTCCCTCTC GGCCAGTGCT CTGTCCCTCA TGATCCCAGG AGATATGTTC 
GCTGTATCTC CATTGGGAAG CCCAATGTCC CCACACTCCC TGTCTTCAGA CCCTTCTTCT 
TCACGGGATT CCTCTCCCAG CCGAGACTCT TCTGCAGCAT CTGCCAGTCC GCATCAGCCC 
ATTGTCATCC ACAGCTCAGG CAAGAACTAT GGGTTCACCA TCCGTGCTAT CCGCGTGTAC 
GTGGGGGACA GTGACATCTA CACAGTGCAC CATATCGTCT GGAACGTAGA AGAAGGAAGT 
CCCGCATACC AGGCAGGACT GAAGGCCGGG GATCTGATCA CACACATCAA CGGAGAGCCG 
GTGCACGGCC TCGTCCACAC GGAAGTTATC GAGCTCCTGC TGAAGAGTGG GAATAAGGTG 
TCTATCACCA CTACTCCATT TGAAAACACA TCAATCAAAA CGGGACCAGC CAGGAGAAAC 
AGTTACAAGG GCCGGATGGT GAGACGAAGC AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA 
AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC AAGCAGCCTT CTCCTTTGCT CCACACCAGC 
CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG TCATCTGGAG AGAGCCTCCC GGGTTCCCCA 
ACTCACAGCT TGTCCCCGAG GTCTCCAACA CCCAGTTATC GTTCTACTCC CGATTTTCCG 
TCAGGTACAA ATTCCTCCCA GAGCAGCTCC CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA 
GGTTCAGGGC ACATCCGGCC CAGCACCCTC CATGGCCTGG CTCCCAAACT CAGCGGGCAG 
CGATACCGCT CTGGAAGACG GAAGTCGGCT GGCAGCATCC CTCTCTCCCC GCTGGCCAGG 
ACACCCTCTC CCACTCCACA GCCTACCTCT CCTCAGCGTT CACCATCCCC ACTGTTGGGA 
CACTCACTGG GCAATGCCAA GATCACTCAG GCCTTTCCTA GCAAGATGCA CTCTCCCCCC 
ACAATCGTCA GACACATCGT GAGGCCCAAG AGTGCAGAGC CGCCCCGCTC CCCACTGCTG 
AAACGGGTGC AGTCGGAGGA AAAGTTGTCA CCCTCCTATG GCAGTGACAA GAAGCTTCTG 
TGCTCCCGCA AGCATAGCCT AGAGGTGACA CAAGAGGAGG TACAGAGGGA GCAGTGTCAG 
CGGGAAGTGA CACTGCAGAG CCTGGAAGAG AATGTGTGTG ACGCTCCTTC CCTCAGTCGG 
GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA 
GAGTCTGTGG ATGACCTGGA CCGGGACAAG CTGAAAGCCA AGGTTGTCGT AAAGAAACCA 
GAAGAGAAAC ATGAATCGCA CCAGAAACCT CACAGCCTTG GTGGTGATTC GGAAAGCTAT 
GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA AAAGTGTACT CCAAGGGGTT GGAAAGGTCA 
GGCCATTTTG AAAACACATC AGCAGAGTTG CCTTCTGTGG GCAGCCTGCT GAAGGACACT 
CTTCACAAGC AGGCCAGCGT GAGGGCCAGC GAGGGGGTGA CCTCAGACGG GGCAGCTTGC 
AGCCTGACAC CAGGGGAGCA CAGCCAGTCT CTAGGTGACT TTAAGCGGGC CTCAGCTTCT 
GGCATTCTCC ATGATAGTGT GTGCCCCATC TCTGATAGGC CTGCTCCTGG AAAGGTTGAA 
TACTCGGAGA AGGCCTCTCA GGCCAAAGAG CTCCTTCGAA GTGAAAAACT AGACAGCAAG 
CTGGCCAATA TTGATTACCT CAGAAAGAAA ATGTCATTGG ATGACAAAGA TGACAGCCAC 
TGTGCCATCC TGAAACCCAA GATAACATCT AGCGCCCATG AATGTCTGCC AGGGAACCCC 
ATACGGCCCA TGGCAGGGCA ACAAGAGACC CCGCCAGCCT CTGAAAACCG GGCATTCATC 
AACAGTACCC ACACACCTCA GATGAGTGCA GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT 
GGCCGGGTAG AGAACGGAGG GGAGAAAGCA GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG 
AAGAGCCCCT CCGAGTATAA GCTAGAGGGC AGGTCAGTTT CATGTCTCAA GCCGATCGAG 
GGCACACTGG ACATTGCTCT CCTGTCTGGA CCTCACGCCT CCAAAACAGA GTTGCTTTCC 
CCAGAGCCTG CACAGAGTCC CAGCCCAGGC ATCAACGTGG GACCATGTGT GCCACTAGCT 
CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC TCCACCAGCC TGAGAGAGCC TTCCTCAGCC 
AACTTAAAAG TAAATAAATC TTATCTGCTG GAGCCTCGGT TCCTACCCCC GAGCCGGGCT 
CTCCAGGACT CTCTCGCAGC CTCTGGGCCA GAACCGAAGT CAAAGCCGGA AAGGAAGCTC 
ATTCATCCTT CTGCCAGGAG CCCAGCAACT GTCACAGAGA GCAATCTTCA GCAGAAAGAG 
GGTGGTCCCG CCACACACCA AGACCGCTCC ACTGACACCA GGAACCTCCC TGGCCCAGGG 
CAGACCCTAC ACAATGTGGA CCTACCCAGG CTGTGTACAC GTGCCCCACT CCCACCGGAA 
GGGACGCCCG CAAAGGAGAA GCCATGTCTG AAGGAACCCT CTGCCAAGGT GAAAAGCGAG 
TGGTCTGCCG TGAGGGATGA CGGACACAGA GATCCCTGTG CGAAGCTGTG CCCGGCAGAG 
ACTGGTAAAG CCAGCGACAG TTCCAAACCC CTGCCTTCCG GGGGGAGGAC CCAACCCGAT 
TTCTACAAGC AGACCCAGAC TTCGGAGAAA GCATGGGCGC ATGCAAAAAC AAACCACAAA 
GATAGCCAAG ATGAGGTGAA GTCCCTGGCC AGGGAGGACT CAGCTTCACT TTTATATGAA 
AAGGAGATAG GCCGGGCACG AAAAGGTCCT GAACCCAAAC CGGAAGTTCC TGCTACCCGG 
TGCCCTCCTC AGCCACCAGG AATTGAGGGT GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC 
TCTTTGCAGA AACAGGCTCC CAAAGAGCCA GACAGGAAGG AACAGACTTC GCAAAGGCCT 
GGAGGTAGTG GCCCTCAACA ACCCCCACCC ACCAAAGAGC TGTCTAACTC AGCATCCTGG 
CAGCACGGCA GTTCTCCGAG TCACACTTTA AAGAAGGAGC CCGGGACCAA AGCTGCCGCT 
GCAGAACCAA GCACCAGCCT TCATGACACT CCCCGATCTG CTACAGCCAC CACCACTGCC 
ATTGCCACCA CCACCACTAC CACCAGTGCC GGGCACAGTG ACTGCAGTAG CCATAAGGCC 
CGGCCTGGCC CTGACCCCAG CCCTTCAAAG TCTAAGCACC AAGACAGGTC CCTCTCCTCA 
CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC AAAGAGCCTG TCACTCAACC CCTGGGTGGT 
TCCATCAGAG AAGGCAAGGG TGGCAGCAAG GGTCCAGTGG ACACATTTTC TGCTGTCCTG 
ACCACCCAGG GCAAAGCAAG TGATGTGCTT GTGCAGGGAG AAGGTCGGGT CTCAATCATT 
GTCCACACTG AAGAGTGTCC TCTCGATGCC AAACTGAAAA ACACCAATGG AGGGTGTCCC 
CCAGAGATGC AGGCGAAGCA TCCACCCAGA CAAGGACATC TCAGTGAAGC AGCAGACCAG 
AAGCCACTCA TTGCTGGTGA GAAGCAAAGC CCGTCTCCAA AGCATCCCAA ACCATCCACT 
GTGAAAGATT ACCCCAGTCT GTGCAGACAG ACAGACAGAA GCCCAAGCCA TCAGGCTACC 
ACTGGGGACA GGAAGGCAGA AGGAAAGAAA TGCACAGACG CACTTTATGT CGCAGCCCCA 
GAGGGCTACA AGCCAGAGGC CAGCCCTTCT CTCCACCACG GCGAGACCGG ACTCAGAGGC 
TCAGAGAGGC CACCCATGGG CATGGGGAAG GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA 
GGTCCCCAGA AGTCACTGGC TGAAACAGGC AAGCCCAGCG GTATGAAAAG GTCACCCTCT 
GCCACCGTGC AGAGCTCTCT CCGCTCAGCT GCCCCCCCAG AAAAGTCTCT GAGTTACTCA 
GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC 
TCATCTGCCA AGGCTACAGG GGGGACCTCA GAGTTCCCAG CCCCCAGCAG CAGGGACCAC 
AGGAAGCTTC AGTCTGGAGG AGACGGCCGA AGCCAAATGA TAAAGAGTGA CTCTCTGCCC 
TCCTTCCGCC TCTCCACCTC TGCTCTGGAG TCACATTTCC AGGATCCACA GGTGCCCATC 
GCATCAGGCC ACCGAGGCAG GGCACTGTCA GTAACTGCTG CCACAGGAGA ACCCAAAGGG 
AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG AAACAGAATG CGTGCAGAGA GGCGACCAGA 
GCACCCCCAG CCCCAAGCAC AGATCGCTCC CTCCCTCTTT CCTCAGAGAA AGACTTCGTG 
GTTCGGCAGA GAAGGGGCAA GGAGACCTTA AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517768.1
CDS	776..8035
Translation	MDESSILRRRGLQKELSLPRRGSLCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_006517705.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517705.3

ORF Insert Sequence:

ATGGATGAGT CCAGCATTCT GAGACGGAGA GGGCTCCAGA AGGAGCTGAG CCTCCCCCGA 
AGAGGAAGTC TTTGCCGAAC AAGCAACCGG AAGAGTTTGA TAGGCAATGG CCAGTCTCCA 
GCACTGCCTC GACCACACTC ACCTCTCTCT GCTCATGCAG GAAATAGCCC TCAAGACAGT 
CCAAGGAATT TCTCCCCCAG TGCCTCAGCC CATTTCTCAT TTGCGAGGAG AACGGATGGG 
CGCCGCTGGT CTCTGGCTTC TCTCCCTTCC TCAGGCTATG GGACAAATAC CCCAAGCTCC 
ACCGTCTCCT CATCCTGTTC TTCCCAGGAG AAGTTGCACC AGCTACCATA CCAGCCAACC 
CCAGATGAAT TACACTTCTT ATCCAAACAC TTCTGCACAA CAGAAAGTAT CGCCACTGAG 
AACCGGTGCA GAAACACACC CATGCGTCCA CGTTCCCGGA GTCTCAGCCC TGGACGGTCC 
CCCGCCTGCT GTGACCATGA AATAATTATG ATGAACCATG TCTACAAAGA AAGGTTCCCA 
AAGGCCACAG CTCAGATGGA AGAGCGTCTG AAGGAGATCA TCACCAGCTA CTCTCCAGAC 
CATGTTCTCC CCTTGGCAGA TGGGGTACTT AGTTTCACTC ACCATCAGAT CATTGAGCTG 
GCTCGGGACT GTTTGGATAA ATCTCACCAG GGTCTCATCA CGTCGAGATA CTTTTTTGAG 
TTGCAGCACA AACTGGACAA GTTGCTCCAG GAGGCTCACG ATCGCTCTGA AAGTGGAGAA 
CTGGCATTTA TCAAGCAACT AGTCCGAAAG ATCCTAATTG TCATTGCCCG CCCCGCTCGG 
TTATTGGAGT GTTTGGAATT TGATCCTGAA GAATTTTATT ACCTATTGGA AGCTGCAGAA 
GGCCATGCCA AAGAAGGCCA AGGAATCAAA ACTGACATCC CTAGGTATAT CATCAGCCAG 
CTGGGACTCA ATAAGGACCC TTTAGAAGAA ATGGCTCAGT TGGGGAATTA CGACAGTAGG 
ACAGCAGAGA CACCAGAGAT GGATGAGTCA GTGAGTAGCT CAAATACTTC CCTGAGACTT 
CGAAGGAAAC CCCGAGAGAG TGATTTTGAA ACAATTAAAT TGATCAGCAA TGGAGCCTAC 
GGGGCAGTCT ACTTTGTTCG GCACAAAGAG TCCCGCCAGA GGTTTGCCAT GAAGAAGATC 
AACAAGCAGA ACCTCATCCT TCGGAACCAG ATCCAGCAGG CCTTCGTGGA GCGAGACATC 
CTGACTTTCG CAGAGAACCC CTTTGTGGTC AGCATGTATT GCTCCTTTGA AACGAGGCGT 
CACTTATGCA TGGTCATGGA GTATGTAGAA GGGGGAGACT GTGCGACCCT AATGAAAAAC 
ATGGGACCTC TCCCAGTTGA TATGGCCAGA ATGTATTTCG CCGAGACCGT CTTGGCCTTG 
GAGTACCTGC ATAATTACGG AATCGTACAC AGGGACTTGA AGCCAGACAA CCTGTTGGTC 
ACCTCCATGG GGCACATAAA ACTGACTGAC TTCGGCTTGT CTAAGGTGGG ATTAATGAGC 
ATGACCACCA ATCTCTATGA AGGCCACATA GAGAAGGACG CTCGAGAGTT CTTAGATAAA 
CAGGTCTGTG GTACACCTGA GTACATTGCC CCCGAGGTGA TTCTGAGACA GGGCTACGGG 
AAACCCGTGG ACTGGTGGGC CATGGGCATT ATCCTCTATG AATTTCTGGT CGGATGTGTG 
CCTTTCTTTG GGGACACTCC AGAAGAGCTA TTTGGACAAG TCATCAGTGA TGAAATCAAC 
TGGCCTGAAA AGGACGAGGC CCCTCCTCCA GACGCTCAGG AGCTGATTAC CTTGCTCCTC 
AGGCAGAATC CGCTGGAGAG GCTGGGAACA GGTGGAGCCT ATGAAGTGAA GCAGCATCGT 
TTCTTCCGCT CCTTAGACTG GAACAGTTTG CTGAGACAGA AGGCGGAATT TATTCCCCAA 
CTGGAATCGG AGGATGACAC AAGTTATTTT GATACTCGGT CAGAGAAGTA TCATCACATG 
GAGACGGAGG AGGAGGACGA TACAAACGAT GAGGACTTCA CCGTGGAGAT AAGGCAGTTT 
TCTTCCTGTT CACACAGGTT TTCAAAAGTT TTCAGCAGTA TAGATCGCAT AACTCAAAAT 
TCAGGAGAAG ACAAAGATGA CTCTGAGGAC AAGACCAAAA GCACAACGTT GCCATCCACA 
GAGACACTCA GCTGGAGTTC CGAATACTCT GAAATGCAAC AGTTATCGAC CTCCAACTCT 
TCAGATACTG AAAGCAACAG GTGCAAACTC AGCTCTGGCT TGCTCCCCAA GCTGGCTATT 
TCGACAGATG GGGAACAAGA TGAGGCTGTC CCTTGCTCTG GAGACCCCAG AGAGGAGCCA 
GAGAAACCTG TCCCTCCCTC TGAGGAGTGT ACTCAGGAGG AGCCCGAGGT CACCACCCCA 
GCCAGCACCA TCAGCAGTTC CACACTGTCA GTTGGCAGTT TTTCAGAGCA CTTGGATCAG 
ATAAATGGGC GAAGCGAGTG TGTGGACAGT ACAGATAATT CCTCAAAGCC ATCCAGTGAA 
CCCACTTCTC ACGTGGCTCG ACAGCGCTTA GAAAGCACAG AGAAAAAGAA AATTTCTGGG 
AAAGTCACAA AGTCCCTCTC GGCCAGTGCT CTGTCCCTCA TGATCCCAGG AGATATGTTC 
GCTGTATCTC CATTGGGAAG CCCAATGTCC CCACACTCCC TGTCTTCAGA CCCTTCTTCT 
TCACGGGATT CCTCTCCCAG CCGAGACTCT TCTGCAGCAT CTGCCAGTCC GCATCAGCCC 
ATTGTCATCC ACAGCTCAGG CAAGAACTAT GGGTTCACCA TCCGTGCTAT CCGCGTGTAC 
GTGGGGGACA GTGACATCTA CACAGTGCAC CATATCGTCT GGAACGTAGA AGAAGGAAGT 
CCCGCATACC AGGCAGGACT GAAGGCCGGG GATCTGATCA CACACATCAA CGGAGAGCCG 
GTGCACGGCC TCGTCCACAC GGAAGTTATC GAGCTCCTGC TGAAGAGTGG GAATAAGGTG 
TCTATCACCA CTACTCCATT TGAAAACACA TCAATCAAAA CGGGACCAGC CAGGAGAAAC 
AGTTACAAGG GCCGGATGGT GAGACGAAGC AAGAAGTCCA AGAAGAAGGA GAGTCTAGAA 
AGGAGGAGAT CTCTCTTCAA GAAGCTGGCC AAGCAGCCTT CTCCTTTGCT CCACACCAGC 
CGAAGTTTCT CCTGCTTAAA CCGGTCCCTG TCATCTGGAG AGAGCCTCCC GGGTTCCCCA 
ACTCACAGCT TGTCCCCGAG GTCTCCAACA CCCAGTTATC GTTCTACTCC CGATTTTCCG 
TCAGGTACAA ATTCCTCCCA GAGCAGCTCC CCAAGTTCAA GTGCCCCCAA TTCTCCAGCA 
GGTTCAGGGC ACATCCGGCC CAGCACCCTC CATGGCCTGG CTCCCAAACT CAGCGGGCAG 
CGATACCGCT CTGGAAGACG GAAGTCGGCT GGCAGCATCC CTCTCTCCCC GCTGGCCAGG 
ACACCCTCTC CCACTCCACA GCCTACCTCT CCTCAGCGTT CACCATCCCC ACTGTTGGGA 
CACTCACTGG GCAATGCCAA GATCACTCAG GCCTTTCCTA GCAAGATGCA CTCTCCCCCC 
ACAATCGTCA GACACATCGT GAGGCCCAAG AGTGCAGAGC CGCCCCGCTC CCCACTGCTG 
AAACGGGTGC AGTCGGAGGA AAAGTTGTCA CCCTCCTATG GCAGTGACAA GAAGCTTCTG 
TGCTCCCGCA AGCATAGCCT AGAGGTGACA CAAGAGGAGG TACAGAGGGA GCAGTGTCAG 
CGGGAAGTGA CACTGCAGAG CCTGGAAGAG AATGTGTGTG ACGCTCCTTC CCTCAGTCGG 
GCCAGGCCAG TGGAGCAAGG CTGTCTGAAA CGCCCCGTGT CCCGGAAGGT GGGCAGGCAA 
GAGTCTGTGG ATGACCTGGA CCGGGACAAG CTGAAAGCCA AGGTTGTCGT AAAGAAACCA 
GAAGAGAAAC ATGAATCGCA CCAGAAACCT CACAGCCTTG GTGGTGATTC GGAAAGCTAT 
GCTCTCTTCA GGCTAGAGGA GAGAGAGAAA AAAGTGTACT CCAAGGGGTT GGAAAGGTCA 
GGCCATTTTG AAAACACATC AGCAGAGTTG CCTTCTGTGG GCAGCCTGCT GAAGGACACT 
CTTCACAAGC AGGCCAGCGT GAGGGCCAGC GAGGGGGTGA CCTCAGACGG GGCAGCTTGC 
AGCCTGACAC CAGGGGAGCA CAGCCAGTCT CTAGGTGACT TTAAGCGGGC CTCAGCTTCT 
GGCATTCTCC ATGATAGTGT GTGCCCCATC TCTGATAGGC CTGCTCCTGG AAAGGTTGAA 
TACTCGGAGA AGGCCTCTCA GGCCAAAGAG CTCCTTCGAA GTGAAAAACT AGACAGCAAG 
CTGGCCAATA TTGATTACCT CAGAAAGAAA ATGTCATTGG ATGACAAAGA TGACAGCCAC 
TGTGCCATCC TGAAACCCAA GATAACATCT AGCGCCCATG AATGTCTGCC AGGGAACCCC 
ATACGGCCCA TGGCAGGGCA ACAAGAGACC CCGCCAGCCT CTGAAAACCG GGCATTCATC 
AACAGTACCC ACACACCTCA GATGAGTGCA GTTTCCTTTG TTCCTCTCAA AGCCTTAGCT 
GGCCGGGTAG AGAACGGAGG GGAGAAAGCA GGCTTAGCTG CTCCCGAGTC CCCTGTCAGG 
AAGAGCCCCT CCGAGTATAA GCTAGAGGGC AGGTCAGTTT CATGTCTCAA GCCGATCGAG 
GGCACACTGG ACATTGCTCT CCTGTCTGGA CCTCACGCCT CCAAAACAGA GTTGCTTTCC 
CCAGAGCCTG CACAGAGTCC CAGCCCAGGC ATCAACGTGG GACCATGTGT GCCACTAGCT 
CTTCCTGGGA GCAGTGGGAA AAAGGGAGAC TCCACCAGCC TGAGAGAGCC TTCCTCAGCC 
AACTTAAAAG TAAATAAATC TTATCTGCTG GAGCCTCGGT TCCTACCCCC GAGCCGGGCT 
CTCCAGGACT CTCTCGCAGC CTCTGGGCCA GAACCGAAGT CAAAGCCGGA AAGGAAGCTC 
ATTCATCCTT CTGCCAGGAG CCCAGCAACT GTCACAGAGA GCAATCTTCA GCAGAAAGAG 
GGTGGTCCCG CCACACACCA AGACCGCTCC ACTGACACCA GGAACCTCCC TGGCCCAGGG 
CAGACCCTAC ACAATGTGGA CCTACCCAGG CTGTGTACAC GTGCCCCACT CCCACCGGAA 
GGGACGCCCG CAAAGGAGAA GCCATGTCTG AAGGAACCCT CTGCCAAGGT GAAAAGCGAG 
TGGTCTGCCG TGAGGGATGA CGGACACAGA GATCCCTGTG CGAAGCTGTG CCCGGCAGAG 
ACTGGTAAAG CCAGCGACAG TTCCAAACCC CTGCCTTCCG GGGGGAGGAC CCAACCCGAT 
TTCTACAAGC AGACCCAGAC TTCGGAGAAA GCATGGGCGC ATGCAAAAAC AAACCACAAA 
GATAGCCAAG ATGAGGTGAA GTCCCTGGCC AGGGAGGACT CAGCTTCACT TTTATATGAA 
AAGGAGATAG GCCGGGCACG AAAAGGTCCT GAACCCAAAC CGGAAGTTCC TGCTACCCGG 
TGCCCTCCTC AGCCACCAGG AATTGAGGGT GAGAAGCGAG AAAAGCTCTC CGCTGCCCCC 
TCTTTGCAGA AACAGGCTCC CAAAGAGCCA GACAGGAAGG AACAGACTTC GCAAAGGCCT 
GGAGGTAGTG GCCCTCAACA ACCCCCACCC ACCAAAGAGC TGTCTAACTC AGCATCCTGG 
CAGCACGGCA GTTCTCCGAG TCACACTTTA AAGAAGGAGC CCGGGACCAA AGCTGCCGCT 
GCAGAACCAA GCACCAGCCT TCATGACACT CCCCGATCTG CTACAGCCAC CACCACTGCC 
ATTGCCACCA CCACCACTAC CACCAGTGCC GGGCACAGTG ACTGCAGTAG CCATAAGGCC 
CGGCCTGGCC CTGACCCCAG CCCTTCAAAG TCTAAGCACC AAGACAGGTC CCTCTCCTCA 
CAGAAGCTGA GTGCTGGCTC TGCAAAAGGC AAAGAGCCTG TCACTCAACC CCTGGGTGGT 
TCCATCAGAG AAGGCAAGGG TGGCAGCAAG GGTCCAGTGG ACACATTTTC TGCTGTCCTG 
ACCACCCAGG GCAAAGCAAG TGATGTGCTT GTGCAGGGAG AAGGTCGGGT CTCAATCATT 
GTCCACACTG AAGAGTGTCC TCTCGATGCC AAACTGAAAA ACACCAATGG AGGGTGTCCC 
CCAGAGATGC AGGCGAAGCA TCCACCCAGA CAAGGACATC TCAGTGAAGC AGCAGACCAG 
AAGCCACTCA TTGCTGGTGA GAAGCAAAGC CCGTCTCCAA AGCATCCCAA ACCATCCACT 
GTGAAAGATT ACCCCAGTCT GTGCAGACAG ACAGACAGAA GCCCAAGCCA TCAGGCTACC 
ACTGGGGACA GGAAGGCAGA AGGAAAGAAA TGCACAGACG CACTTTATGT CGCAGCCCCA 
GAGGGCTACA AGCCAGAGGC CAGCCCTTCT CTCCACCACG GCGAGACCGG ACTCAGAGGC 
TCAGAGAGGC CACCCATGGG CATGGGGAAG GGCTTCTCTG AGCCCAAGGG GAAAGGGCCA 
GGTCCCCAGA AGTCACTGGC TGAAACAGGC AAGCCCAGCG GTATGAAAAG GTCACCCTCT 
GCCACCGTGC AGAGCTCTCT CCGCTCAGCT GCCCCCCCAG AAAAGTCTCT GAGTTACTCA 
GCCAGCTTTC CCGAGGCCCA GCCTGGAGTG CGAGAGGTCC CTGCAGCCAA CAGCAGCCCC 
TCATCTGCCA AGGCTACAGG GGGGACCTCA GAGTTCCCAG CCCCCAGCAG CAGGGACCAC 
AGGAAGCTTC AGTCTGGAGG AGACGGCCGA AGCCAAATGA TAAAGAGTGA CTCTCTGCCC 
TCCTTCCGCC TCTCCACCTC TGCTCTGGAG TCACATTTCC AGGATCCACA GGTGCCCATC 
GCATCAGGCC ACCGAGGCAG GGCACTGTCA GTAACTGCTG CCACAGGAGA ACCCAAAGGG 
AGAGAGCTCG CCCAGCCTCC CCCAGTCAGG AAACAGAATG CGTGCAGAGA GGCGACCAGA 
GCACCCCCAG CCCCAAGCAC AGATCGCTCC CTCCCTCTTT CCTCAGAGAA AGACTTCGTG 
GTTCGGCAGA GAAGGGGCAA GGAGACCTTA AGGAGCAGTC CTCACAAAAA GGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_006517706.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517769.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X17, mRNA.

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CloneID	OMu49082
Clone ID Related Accession (Same CDS sequence)	XM_006517706.3
Accession Version	XM_006517706.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCCC AGCGGGAAAG ACTACAGATT CCGGGGCTGA CCTTGGATTG CCGAACAAGC 
AACCGGAAGA GTTTGATAGG CAATGGCCAG TCTCCAGCAC TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GACAGTCCAA GGAATTTCTC CCCCAGTGCC 
TCAGCCCATT TCTCATTTGC GAGGAGAAAT GACAGAACGG ATGGGCGCCG CTGGTCTCTG 
GCTTCTCTCC CTTCCTCAGG CTATGGGACA AATACCCCAA GCTCCACCGT CTCCTCATCC 
TGTTCTTCCC AGGAGAAGTT GCACCAGCTA CCATACCAGC CAACCCCAGA TGAATTACAC 
TTCTTATCCA AACACTTCTG CACAACAGAA AGTATCGCCA CTGAGAACCG GTGCAGAAAC 
ACACCCATGC GTCCACGTTC CCGGAGTCTC AGCCCTGGAC GGTCCCCCGC CTGCTGTGAC 
CATGAAATAA TTATGATGAA CCATGTCTAC AAAGAAAGGT TCCCAAAGGC CACAGCTCAG 
ATGGAAGAGC GTCTGAAGGA GATCATCACC AGCTACTCTC CAGACCATGT TCTCCCCTTG 
GCAGATGGGG TACTTAGTTT CACTCACCAT CAGATCATTG AGCTGGCTCG GGACTGTTTG 
GATAAATCTC ACCAGGGTCT CATCACGTCG AGATACTTTT TTGAGTTGCA GCACAAACTG 
GACAAGTTGC TCCAGGAGGC TCACGATCGC TCTGAAAGTG GAGAACTGGC ATTTATCAAG 
CAACTAGTCC GAAAGATCCT AATTGTCATT GCCCGCCCCG CTCGGTTATT GGAGTGTTTG 
GAATTTGATC CTGAAGAATT TTATTACCTA TTGGAAGCTG CAGAAGGCCA TGCCAAAGAA 
GGCCAAGGAA TCAAAACTGA CATCCCTAGG TATATCATCA GCCAGCTGGG ACTCAATAAG 
GACCCTTTAG AAGAAATGGC TCAGTTGGGG AATTACGACA GTAGGACAGC AGAGACACCA 
GAGATGGATG AGTCAGTGAG TAGCTCAAAT ACTTCCCTGA GACTTCGAAG GAAACCCCGA 
GAGAGTGATT TTGAAACAAT TAAATTGATC AGCAATGGAG CCTACGGGGC AGTCTACTTT 
GTTCGGCACA AAGAGTCCCG CCAGAGGTTT GCCATGAAGA AGATCAACAA GCAGAACCTC 
ATCCTTCGGA ACCAGATCCA GCAGGCCTTC GTGGAGCGAG ACATCCTGAC TTTCGCAGAG 
AACCCCTTTG TGGTCAGCAT GTATTGCTCC TTTGAAACGA GGCGTCACTT ATGCATGGTC 
ATGGAGTATG TAGAAGGGGG AGACTGTGCG ACCCTAATGA AAAACATGGG ACCTCTCCCA 
GTTGATATGG CCAGAATGTA TTTCGCCGAG ACCGTCTTGG CCTTGGAGTA CCTGCATAAT 
TACGGAATCG TACACAGGGA CTTGAAGCCA GACAACCTGT TGGTCACCTC CATGGGGCAC 
ATAAAACTGA CTGACTTCGG CTTGTCTAAG GTGGGATTAA TGAGCATGAC CACCAATCTC 
TATGAAGGCC ACATAGAGAA GGACGCTCGA GAGTTCTTAG ATAAACAGGT CTGTGGTACA 
CCTGAGTACA TTGCCCCCGA GGTGATTCTG AGACAGGGCT ACGGGAAACC CGTGGACTGG 
TGGGCCATGG GCATTATCCT CTATGAATTT CTGGTCGGAT GTGTGCCTTT CTTTGGGGAC 
ACTCCAGAAG AGCTATTTGG ACAAGTCATC AGTGATGAAA TCAACTGGCC TGAAAAGGAC 
GAGGCCCCTC CTCCAGACGC TCAGGAGCTG ATTACCTTGC TCCTCAGGCA GAATCCGCTG 
GAGAGGCTGG GAACAGGTGG AGCCTATGAA GTGAAGCAGC ATCGTTTCTT CCGCTCCTTA 
GACTGGAACA GTTTGCTGAG ACAGAAGGCG GAATTTATTC CCCAACTGGA ATCGGAGGAT 
GACACAAGTT ATTTTGATAC TCGGTCAGAG AAGTATCATC ACATGGAGAC GGAGGAGGAG 
GACGATACAA ACGATGAGGA CTTCACCGTG GAGATAAGGC AGTTTTCTTC CTGTTCACAC 
AGGTTTTCAA AAGTTTTCAG CAGTATAGAT CGCATAACTC AAAATTCAGG AGAAGACAAA 
GATGACTCTG AGGACAAGAC CAAAAGCACA ACGTTGCCAT CCACAGAGAC ACTCAGCTGG 
AGTTCCGAAT ACTCTGAAAT GCAACAGTTA TCGACCTCCA ACTCTTCAGA TACTGAAAGC 
AACAGGTGCA AACTCAGCTC TGGCTTGCTC CCCAAGCTGG CTATTTCGAC AGATGGGGAA 
CAAGATGAGG CTGTCCCTTG CTCTGGAGAC CCCAGAGAGG AGCCAGAGAA ACCTGTCCCT 
CCCTCTGAGG AGTGTACTCA GGAGGAGCCC GAGGTCACCA CCCCAGCCAG CACCATCAGC 
AGTTCCACAC TGTCAGTTGG CAGTTTTTCA GAGCACTTGG ATCAGATAAA TGGGCGAAGC 
GAGTGTGTGG ACAGTACAGA TAATTCCTCA AAGCCATCCA GTGAACCCAC TTCTCACGTG 
GCTCGACAGC GCTTAGAAAG CACAGAGAAA AAGAAAATTT CTGGGAAAGT CACAAAGTCC 
CTCTCGGCCA GTGCTCTGTC CCTCATGATC CCAGGAGATA TGTTCGCTGT ATCTCCATTG 
GGAAGCCCAA TGTCCCCACA CTCCCTGTCT TCAGACCCTT CTTCTTCACG GGATTCCTCT 
CCCAGCCGAG ACTCTTCTGC AGCATCTGCC AGTCCGCATC AGCCCATTGT CATCCACAGC 
TCAGGCAAGA ACTATGGGTT CACCATCCGT GCTATCCGCG TGTACGTGGG GGACAGTGAC 
ATCTACACAG TGCACCATAT CGTCTGGAAC GTAGAAGAAG GAAGTCCCGC ATACCAGGCA 
GGACTGAAGG CCGGGGATCT GATCACACAC ATCAACGGAG AGCCGGTGCA CGGCCTCGTC 
CACACGGAAG TTATCGAGCT CCTGCTGAAG AGTGGGAATA AGGTGTCTAT CACCACTACT 
CCATTTGAAA ACACATCAAT CAAAACGGGA CCAGCCAGGA GAAACAGTTA CAAGGGCCGG 
ATGGTGAGAC GAAGCAAGAA GTCCAAGAAG AAGGAGAGTC TAGAAAGGAG GAGATCTCTC 
TTCAAGAAGC TGGCCAAGCA GCCTTCTCCT TTGCTCCACA CCAGCCGAAG TTTCTCCTGC 
TTAAACCGGT CCCTGTCATC TGGAGAGAGC CTCCCGGGTT CCCCAACTCA CAGCTTGTCC 
CCGAGGTCTC CAACACCCAG TTATCGTTCT ACTCCCGATT TTCCGTCAGG TACAAATTCC 
TCCCAGAGCA GCTCCCCAAG TTCAAGTGCC CCCAATTCTC CAGCAGGTTC AGGGCACATC 
CGGCCCAGCA CCCTCCATGG CCTGGCTCCC AAACTCAGCG GGCAGCGATA CCGCTCTGGA 
AGACGGAAGT CGGCTGGCAG CATCCCTCTC TCCCCGCTGG CCAGGACACC CTCTCCCACT 
CCACAGCCTA CCTCTCCTCA GCGTTCACCA TCCCCACTGT TGGGACACTC ACTGGGCAAT 
GCCAAGATCA CTCAGGCCTT TCCTAGCAAG ATGCACTCTC CCCCCACAAT CGTCAGACAC 
ATCGTGAGGC CCAAGAGTGC AGAGCCGCCC CGCTCCCCAC TGCTGAAACG GGTGCAGTCG 
GAGGAAAAGT TGTCACCCTC CTATGGCAGT GACAAGAAGC TTCTGTGCTC CCGCAAGCAT 
AGCCTAGAGG TGACACAAGA GGAGGTACAG AGGGAGCAGT GTCAGCGGGA AGTGACACTG 
CAGAGCCTGG AAGAGAATGT GTGTGACGCT CCTTCCCTCA GTCGGGCCAG GCCAGTGGAG 
CAAGGCTGTC TGAAACGCCC CGTGTCCCGG AAGGTGGGCA GGCAAGAGTC TGTGGATGAC 
CTGGACCGGG ACAAGCTGAA AGCCAAGGTT GTCGTAAAGA AACCAGAAGA GAAACATGAA 
TCGCACCAGA AACCTCACAG CCTTGGTGGT GATTCGGAAA GCTATGCTCT CTTCAGGCTA 
GAGGAGAGAG AGAAAAAAGT GTACTCCAAG GGGTTGGAAA GGTCAGGCCA TTTTGAAAAC 
ACATCAGCAG AGTTGCCTTC TGTGGGCAGC CTGCTGAAGG ACACTCTTCA CAAGCAGGCC 
AGCGTGAGGG CCAGCGAGGG GGTGACCTCA GACGGGGCAG CTTGCAGCCT GACACCAGGG 
GAGCACAGCC AGTCTCTAGG TGACTTTAAG CGGGCCTCAG CTTCTGGCAT TCTCCATGAT 
AGTGTGTGCC CCATCTCTGA TAGGCCTGCT CCTGGAAAGG TTGAATACTC GGAGAAGGCC 
TCTCAGGCCA AAGAGCTCCT TCGAAGTGAA AAACTAGACA GCAAGCTGGC CAATATTGAT 
TACCTCAGAA AGAAAATGTC ATTGGATGAC AAAGATGACA GCCACTGTGC CATCCTGAAA 
CCCAAGATAA CATCTAGCGC CCATGAATGT CTGCCAGGGA ACCCCATACG GCCCATGGCA 
GGGCAACAAG AGACCCCGCC AGCCTCTGAA AACCGGGCAT TCATCAACAG TACCCACACA 
CCTCAGATGA GTGCAGTTTC CTTTGTTCCT CTCAAAGCCT TAGCTGGCCG GGTAGAGAAC 
GGAGGGGAGA AAGCAGGCTT AGCTGCTCCC GAGTCCCCTG TCAGGAAGAG CCCCTCCGAG 
TATAAGCTAG AGGGCAGGTC AGTTTCATGT CTCAAGCCGA TCGAGGGCAC ACTGGACATT 
GCTCTCCTGT CTGGACCTCA CGCCTCCAAA ACAGAGTTGC TTTCCCCAGA GCCTGCACAG 
AGTCCCAGCC CAGGCATCAA CGTGGGACCA TGTGTGCCAC TAGCTCTTCC TGGGAGCAGT 
GGGAAAAAGG GAGACTCCAC CAGCCTGAGA GAGCCTTCCT CAGCCAACTT AAAAGTAAAT 
AAATCTTATC TGCTGGAGCC TCGGTTCCTA CCCCCGAGCC GGGCTCTCCA GGACTCTCTC 
GCAGCCTCTG GGCCAGAACC GAAGTCAAAG CCGGAAAGGA AGCTCATTCA TCCTTCTGCC 
AGGAGCCCAG CAACTGTCAC AGAGAGCAAT CTTCAGCAGA AAGAGGGTGG TCCCGCCACA 
CACCAAGACC GCTCCACTGA CACCAGGAAC CTCCCTGGCC CAGGGCAGAC CCTACACAAT 
GTGGACCTAC CCAGGCTGTG TACACGTGCC CCACTCCCAC CGGAAGGGAC GCCCGCAAAG 
GAGAAGCCAT GTCTGAAGGA ACCCTCTGCC AAGGTGAAAA GCGAGTGGTC TGCCGTGAGG 
GATGACGGAC ACAGAGATCC CTGTGCGAAG CTGTGCCCGG CAGAGACTGG TAAAGCCAGC 
GACAGTTCCA AACCCCTGCC TTCCGGGGGG AGGACCCAAC CCGATTTCTA CAAGCAGACC 
CAGACTTCGG AGAAAGCATG GGCGCATGCA AAAACAAACC ACAAAGATAG CCAAGATGAG 
GTGAAGTCCC TGGCCAGGGA GGACTCAGCT TCACTTTTAT ATGAAAAGGA GATAGGCCGG 
GCACGAAAAG GTCCTGAACC CAAACCGGAA GTTCCTGCTA CCCGGTGCCC TCCTCAGCCA 
CCAGGAATTG AGGGTGAGAA GCGAGAAAAG CTCTCCGCTG CCCCCTCTTT GCAGAAACAG 
GCTCCCAAAG AGCCAGACAG GAAGGAACAG ACTTCGCAAA GGCCTGGAGG TAGTGGCCCT 
CAACAACCCC CACCCACCAA AGAGCTGTCT AACTCAGCAT CCTGGCAGCA CGGCAGTTCT 
CCGAGTCACA CTTTAAAGAA GGAGCCCGGG ACCAAAGCTG CCGCTGCAGA ACCAAGCACC 
AGCCTTCATG ACACTCCCCG ATCTGCTACA GCCACCACCA CTGCCATTGC CACCACCACC 
ACTACCACCA GTGCCGGGCA CAGTGACTGC AGTAGCCATA AGGCCCGGCC TGGCCCTGAC 
CCCAGCCCTT CAAAGTCTAA GCACCAAGAC AGGTCCCTCT CCTCACAGAA GCTGAGTGCT 
GGCTCTGCAA AAGGCAAAGA GCCTGTCACT CAACCCCTGG GTGGTTCCAT CAGAGAAGGC 
AAGGGTGGCA GCAAGGGTCC AGTGGACACA TTTTCTGCTG TCCTGACCAC CCAGGGCAAA 
GCAAGTGATG TGCTTGTGCA GGGAGAAGGT CGGGTCTCAA TCATTGTCCA CACTGAAGAG 
TGTCCTCTCG ATGCCAAACT GAAAAACACC AATGGAGGGT GTCCCCCAGA GATGCAGGCG 
AAGCATCCAC CCAGACAAGG ACATCTCAGT GAAGCAGCAG ACCAGAAGCC ACTCATTGCT 
GGTGAGAAGC AAAGCCCGTC TCCAAAGCAT CCCAAACCAT CCACTGTGAA AGATTACCCC 
AGTCTGTGCA GACAGACAGA CAGAAGCCCA AGCCATCAGG CTACCACTGG GGACAGGAAG 
GCAGAAGGAA AGAAATGCAC AGACGCACTT TATGTCGCAG CCCCAGAGGG CTACAAGCCA 
GAGGCCAGCC CTTCTCTCCA CCACGGCGAG ACCGGACTCA GAGGCTCAGA GAGGCCACCC 
ATGGGCATGG GGAAGGGCTT CTCTGAGCCC AAGGGGAAAG GGCCAGGTCC CCAGAAGTCA 
CTGGCTGAAA CAGGCAAGCC CAGCGGTATG AAAAGGTCAC CCTCTGCCAC CGTGCAGAGC 
TCTCTCCGCT CAGCTGCCCC CCCAGAAAAG TCTCTGAGTT ACTCAGCCAG CTTTCCCGAG 
GCCCAGCCTG GAGTGCGAGA GGTCCCTGCA GCCAACAGCA GCCCCTCATC TGCCAAGGCT 
ACAGGGGGGA CCTCAGAGTT CCCAGCCCCC AGCAGCAGGG ACCACAGGAA GCTTCAGTCT 
GGAGGAGACG GCCGAAGCCA AATGATAAAG AGTGACTCTC TGCCCTCCTT CCGCCTCTCC 
ACCTCTGCTC TGGAGTCACA TTTCCAGGAT CCACAGGTGC CCATCGCATC AGGCCACCGA 
GGCAGGGCAC TGTCAGTAAC TGCTGCCACA GGAGAACCCA AAGGGAGAGA GCTCGCCCAG 
CCTCCCCCAG TCAGGAAACA GAATGCGTGC AGAGAGGCGA CCAGAGCACC CCCAGCCCCA 
AGCACAGATC GCTCCCTCCC TCTTTCCTCA GAGAAAGACT TCGTGGTTCG GCAGAGAAGG 
GGCAAGGAGA CCTTAAGGAG CAGTCCTCAC AAAAAGGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517769.1
CDS	257..7501
Translation	MKAQRERLQIPGLTLDCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_006517706.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517706.3

ORF Insert Sequence:

ATGAAAGCCC AGCGGGAAAG ACTACAGATT CCGGGGCTGA CCTTGGATTG CCGAACAAGC 
AACCGGAAGA GTTTGATAGG CAATGGCCAG TCTCCAGCAC TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GACAGTCCAA GGAATTTCTC CCCCAGTGCC 
TCAGCCCATT TCTCATTTGC GAGGAGAAAT GACAGAACGG ATGGGCGCCG CTGGTCTCTG 
GCTTCTCTCC CTTCCTCAGG CTATGGGACA AATACCCCAA GCTCCACCGT CTCCTCATCC 
TGTTCTTCCC AGGAGAAGTT GCACCAGCTA CCATACCAGC CAACCCCAGA TGAATTACAC 
TTCTTATCCA AACACTTCTG CACAACAGAA AGTATCGCCA CTGAGAACCG GTGCAGAAAC 
ACACCCATGC GTCCACGTTC CCGGAGTCTC AGCCCTGGAC GGTCCCCCGC CTGCTGTGAC 
CATGAAATAA TTATGATGAA CCATGTCTAC AAAGAAAGGT TCCCAAAGGC CACAGCTCAG 
ATGGAAGAGC GTCTGAAGGA GATCATCACC AGCTACTCTC CAGACCATGT TCTCCCCTTG 
GCAGATGGGG TACTTAGTTT CACTCACCAT CAGATCATTG AGCTGGCTCG GGACTGTTTG 
GATAAATCTC ACCAGGGTCT CATCACGTCG AGATACTTTT TTGAGTTGCA GCACAAACTG 
GACAAGTTGC TCCAGGAGGC TCACGATCGC TCTGAAAGTG GAGAACTGGC ATTTATCAAG 
CAACTAGTCC GAAAGATCCT AATTGTCATT GCCCGCCCCG CTCGGTTATT GGAGTGTTTG 
GAATTTGATC CTGAAGAATT TTATTACCTA TTGGAAGCTG CAGAAGGCCA TGCCAAAGAA 
GGCCAAGGAA TCAAAACTGA CATCCCTAGG TATATCATCA GCCAGCTGGG ACTCAATAAG 
GACCCTTTAG AAGAAATGGC TCAGTTGGGG AATTACGACA GTAGGACAGC AGAGACACCA 
GAGATGGATG AGTCAGTGAG TAGCTCAAAT ACTTCCCTGA GACTTCGAAG GAAACCCCGA 
GAGAGTGATT TTGAAACAAT TAAATTGATC AGCAATGGAG CCTACGGGGC AGTCTACTTT 
GTTCGGCACA AAGAGTCCCG CCAGAGGTTT GCCATGAAGA AGATCAACAA GCAGAACCTC 
ATCCTTCGGA ACCAGATCCA GCAGGCCTTC GTGGAGCGAG ACATCCTGAC TTTCGCAGAG 
AACCCCTTTG TGGTCAGCAT GTATTGCTCC TTTGAAACGA GGCGTCACTT ATGCATGGTC 
ATGGAGTATG TAGAAGGGGG AGACTGTGCG ACCCTAATGA AAAACATGGG ACCTCTCCCA 
GTTGATATGG CCAGAATGTA TTTCGCCGAG ACCGTCTTGG CCTTGGAGTA CCTGCATAAT 
TACGGAATCG TACACAGGGA CTTGAAGCCA GACAACCTGT TGGTCACCTC CATGGGGCAC 
ATAAAACTGA CTGACTTCGG CTTGTCTAAG GTGGGATTAA TGAGCATGAC CACCAATCTC 
TATGAAGGCC ACATAGAGAA GGACGCTCGA GAGTTCTTAG ATAAACAGGT CTGTGGTACA 
CCTGAGTACA TTGCCCCCGA GGTGATTCTG AGACAGGGCT ACGGGAAACC CGTGGACTGG 
TGGGCCATGG GCATTATCCT CTATGAATTT CTGGTCGGAT GTGTGCCTTT CTTTGGGGAC 
ACTCCAGAAG AGCTATTTGG ACAAGTCATC AGTGATGAAA TCAACTGGCC TGAAAAGGAC 
GAGGCCCCTC CTCCAGACGC TCAGGAGCTG ATTACCTTGC TCCTCAGGCA GAATCCGCTG 
GAGAGGCTGG GAACAGGTGG AGCCTATGAA GTGAAGCAGC ATCGTTTCTT CCGCTCCTTA 
GACTGGAACA GTTTGCTGAG ACAGAAGGCG GAATTTATTC CCCAACTGGA ATCGGAGGAT 
GACACAAGTT ATTTTGATAC TCGGTCAGAG AAGTATCATC ACATGGAGAC GGAGGAGGAG 
GACGATACAA ACGATGAGGA CTTCACCGTG GAGATAAGGC AGTTTTCTTC CTGTTCACAC 
AGGTTTTCAA AAGTTTTCAG CAGTATAGAT CGCATAACTC AAAATTCAGG AGAAGACAAA 
GATGACTCTG AGGACAAGAC CAAAAGCACA ACGTTGCCAT CCACAGAGAC ACTCAGCTGG 
AGTTCCGAAT ACTCTGAAAT GCAACAGTTA TCGACCTCCA ACTCTTCAGA TACTGAAAGC 
AACAGGTGCA AACTCAGCTC TGGCTTGCTC CCCAAGCTGG CTATTTCGAC AGATGGGGAA 
CAAGATGAGG CTGTCCCTTG CTCTGGAGAC CCCAGAGAGG AGCCAGAGAA ACCTGTCCCT 
CCCTCTGAGG AGTGTACTCA GGAGGAGCCC GAGGTCACCA CCCCAGCCAG CACCATCAGC 
AGTTCCACAC TGTCAGTTGG CAGTTTTTCA GAGCACTTGG ATCAGATAAA TGGGCGAAGC 
GAGTGTGTGG ACAGTACAGA TAATTCCTCA AAGCCATCCA GTGAACCCAC TTCTCACGTG 
GCTCGACAGC GCTTAGAAAG CACAGAGAAA AAGAAAATTT CTGGGAAAGT CACAAAGTCC 
CTCTCGGCCA GTGCTCTGTC CCTCATGATC CCAGGAGATA TGTTCGCTGT ATCTCCATTG 
GGAAGCCCAA TGTCCCCACA CTCCCTGTCT TCAGACCCTT CTTCTTCACG GGATTCCTCT 
CCCAGCCGAG ACTCTTCTGC AGCATCTGCC AGTCCGCATC AGCCCATTGT CATCCACAGC 
TCAGGCAAGA ACTATGGGTT CACCATCCGT GCTATCCGCG TGTACGTGGG GGACAGTGAC 
ATCTACACAG TGCACCATAT CGTCTGGAAC GTAGAAGAAG GAAGTCCCGC ATACCAGGCA 
GGACTGAAGG CCGGGGATCT GATCACACAC ATCAACGGAG AGCCGGTGCA CGGCCTCGTC 
CACACGGAAG TTATCGAGCT CCTGCTGAAG AGTGGGAATA AGGTGTCTAT CACCACTACT 
CCATTTGAAA ACACATCAAT CAAAACGGGA CCAGCCAGGA GAAACAGTTA CAAGGGCCGG 
ATGGTGAGAC GAAGCAAGAA GTCCAAGAAG AAGGAGAGTC TAGAAAGGAG GAGATCTCTC 
TTCAAGAAGC TGGCCAAGCA GCCTTCTCCT TTGCTCCACA CCAGCCGAAG TTTCTCCTGC 
TTAAACCGGT CCCTGTCATC TGGAGAGAGC CTCCCGGGTT CCCCAACTCA CAGCTTGTCC 
CCGAGGTCTC CAACACCCAG TTATCGTTCT ACTCCCGATT TTCCGTCAGG TACAAATTCC 
TCCCAGAGCA GCTCCCCAAG TTCAAGTGCC CCCAATTCTC CAGCAGGTTC AGGGCACATC 
CGGCCCAGCA CCCTCCATGG CCTGGCTCCC AAACTCAGCG GGCAGCGATA CCGCTCTGGA 
AGACGGAAGT CGGCTGGCAG CATCCCTCTC TCCCCGCTGG CCAGGACACC CTCTCCCACT 
CCACAGCCTA CCTCTCCTCA GCGTTCACCA TCCCCACTGT TGGGACACTC ACTGGGCAAT 
GCCAAGATCA CTCAGGCCTT TCCTAGCAAG ATGCACTCTC CCCCCACAAT CGTCAGACAC 
ATCGTGAGGC CCAAGAGTGC AGAGCCGCCC CGCTCCCCAC TGCTGAAACG GGTGCAGTCG 
GAGGAAAAGT TGTCACCCTC CTATGGCAGT GACAAGAAGC TTCTGTGCTC CCGCAAGCAT 
AGCCTAGAGG TGACACAAGA GGAGGTACAG AGGGAGCAGT GTCAGCGGGA AGTGACACTG 
CAGAGCCTGG AAGAGAATGT GTGTGACGCT CCTTCCCTCA GTCGGGCCAG GCCAGTGGAG 
CAAGGCTGTC TGAAACGCCC CGTGTCCCGG AAGGTGGGCA GGCAAGAGTC TGTGGATGAC 
CTGGACCGGG ACAAGCTGAA AGCCAAGGTT GTCGTAAAGA AACCAGAAGA GAAACATGAA 
TCGCACCAGA AACCTCACAG CCTTGGTGGT GATTCGGAAA GCTATGCTCT CTTCAGGCTA 
GAGGAGAGAG AGAAAAAAGT GTACTCCAAG GGGTTGGAAA GGTCAGGCCA TTTTGAAAAC 
ACATCAGCAG AGTTGCCTTC TGTGGGCAGC CTGCTGAAGG ACACTCTTCA CAAGCAGGCC 
AGCGTGAGGG CCAGCGAGGG GGTGACCTCA GACGGGGCAG CTTGCAGCCT GACACCAGGG 
GAGCACAGCC AGTCTCTAGG TGACTTTAAG CGGGCCTCAG CTTCTGGCAT TCTCCATGAT 
AGTGTGTGCC CCATCTCTGA TAGGCCTGCT CCTGGAAAGG TTGAATACTC GGAGAAGGCC 
TCTCAGGCCA AAGAGCTCCT TCGAAGTGAA AAACTAGACA GCAAGCTGGC CAATATTGAT 
TACCTCAGAA AGAAAATGTC ATTGGATGAC AAAGATGACA GCCACTGTGC CATCCTGAAA 
CCCAAGATAA CATCTAGCGC CCATGAATGT CTGCCAGGGA ACCCCATACG GCCCATGGCA 
GGGCAACAAG AGACCCCGCC AGCCTCTGAA AACCGGGCAT TCATCAACAG TACCCACACA 
CCTCAGATGA GTGCAGTTTC CTTTGTTCCT CTCAAAGCCT TAGCTGGCCG GGTAGAGAAC 
GGAGGGGAGA AAGCAGGCTT AGCTGCTCCC GAGTCCCCTG TCAGGAAGAG CCCCTCCGAG 
TATAAGCTAG AGGGCAGGTC AGTTTCATGT CTCAAGCCGA TCGAGGGCAC ACTGGACATT 
GCTCTCCTGT CTGGACCTCA CGCCTCCAAA ACAGAGTTGC TTTCCCCAGA GCCTGCACAG 
AGTCCCAGCC CAGGCATCAA CGTGGGACCA TGTGTGCCAC TAGCTCTTCC TGGGAGCAGT 
GGGAAAAAGG GAGACTCCAC CAGCCTGAGA GAGCCTTCCT CAGCCAACTT AAAAGTAAAT 
AAATCTTATC TGCTGGAGCC TCGGTTCCTA CCCCCGAGCC GGGCTCTCCA GGACTCTCTC 
GCAGCCTCTG GGCCAGAACC GAAGTCAAAG CCGGAAAGGA AGCTCATTCA TCCTTCTGCC 
AGGAGCCCAG CAACTGTCAC AGAGAGCAAT CTTCAGCAGA AAGAGGGTGG TCCCGCCACA 
CACCAAGACC GCTCCACTGA CACCAGGAAC CTCCCTGGCC CAGGGCAGAC CCTACACAAT 
GTGGACCTAC CCAGGCTGTG TACACGTGCC CCACTCCCAC CGGAAGGGAC GCCCGCAAAG 
GAGAAGCCAT GTCTGAAGGA ACCCTCTGCC AAGGTGAAAA GCGAGTGGTC TGCCGTGAGG 
GATGACGGAC ACAGAGATCC CTGTGCGAAG CTGTGCCCGG CAGAGACTGG TAAAGCCAGC 
GACAGTTCCA AACCCCTGCC TTCCGGGGGG AGGACCCAAC CCGATTTCTA CAAGCAGACC 
CAGACTTCGG AGAAAGCATG GGCGCATGCA AAAACAAACC ACAAAGATAG CCAAGATGAG 
GTGAAGTCCC TGGCCAGGGA GGACTCAGCT TCACTTTTAT ATGAAAAGGA GATAGGCCGG 
GCACGAAAAG GTCCTGAACC CAAACCGGAA GTTCCTGCTA CCCGGTGCCC TCCTCAGCCA 
CCAGGAATTG AGGGTGAGAA GCGAGAAAAG CTCTCCGCTG CCCCCTCTTT GCAGAAACAG 
GCTCCCAAAG AGCCAGACAG GAAGGAACAG ACTTCGCAAA GGCCTGGAGG TAGTGGCCCT 
CAACAACCCC CACCCACCAA AGAGCTGTCT AACTCAGCAT CCTGGCAGCA CGGCAGTTCT 
CCGAGTCACA CTTTAAAGAA GGAGCCCGGG ACCAAAGCTG CCGCTGCAGA ACCAAGCACC 
AGCCTTCATG ACACTCCCCG ATCTGCTACA GCCACCACCA CTGCCATTGC CACCACCACC 
ACTACCACCA GTGCCGGGCA CAGTGACTGC AGTAGCCATA AGGCCCGGCC TGGCCCTGAC 
CCCAGCCCTT CAAAGTCTAA GCACCAAGAC AGGTCCCTCT CCTCACAGAA GCTGAGTGCT 
GGCTCTGCAA AAGGCAAAGA GCCTGTCACT CAACCCCTGG GTGGTTCCAT CAGAGAAGGC 
AAGGGTGGCA GCAAGGGTCC AGTGGACACA TTTTCTGCTG TCCTGACCAC CCAGGGCAAA 
GCAAGTGATG TGCTTGTGCA GGGAGAAGGT CGGGTCTCAA TCATTGTCCA CACTGAAGAG 
TGTCCTCTCG ATGCCAAACT GAAAAACACC AATGGAGGGT GTCCCCCAGA GATGCAGGCG 
AAGCATCCAC CCAGACAAGG ACATCTCAGT GAAGCAGCAG ACCAGAAGCC ACTCATTGCT 
GGTGAGAAGC AAAGCCCGTC TCCAAAGCAT CCCAAACCAT CCACTGTGAA AGATTACCCC 
AGTCTGTGCA GACAGACAGA CAGAAGCCCA AGCCATCAGG CTACCACTGG GGACAGGAAG 
GCAGAAGGAA AGAAATGCAC AGACGCACTT TATGTCGCAG CCCCAGAGGG CTACAAGCCA 
GAGGCCAGCC CTTCTCTCCA CCACGGCGAG ACCGGACTCA GAGGCTCAGA GAGGCCACCC 
ATGGGCATGG GGAAGGGCTT CTCTGAGCCC AAGGGGAAAG GGCCAGGTCC CCAGAAGTCA 
CTGGCTGAAA CAGGCAAGCC CAGCGGTATG AAAAGGTCAC CCTCTGCCAC CGTGCAGAGC 
TCTCTCCGCT CAGCTGCCCC CCCAGAAAAG TCTCTGAGTT ACTCAGCCAG CTTTCCCGAG 
GCCCAGCCTG GAGTGCGAGA GGTCCCTGCA GCCAACAGCA GCCCCTCATC TGCCAAGGCT 
ACAGGGGGGA CCTCAGAGTT CCCAGCCCCC AGCAGCAGGG ACCACAGGAA GCTTCAGTCT 
GGAGGAGACG GCCGAAGCCA AATGATAAAG AGTGACTCTC TGCCCTCCTT CCGCCTCTCC 
ACCTCTGCTC TGGAGTCACA TTTCCAGGAT CCACAGGTGC CCATCGCATC AGGCCACCGA 
GGCAGGGCAC TGTCAGTAAC TGCTGCCACA GGAGAACCCA AAGGGAGAGA GCTCGCCCAG 
CCTCCCCCAG TCAGGAAACA GAATGCGTGC AGAGAGGCGA CCAGAGCACC CCCAGCCCCA 
AGCACAGATC GCTCCCTCCC TCTTTCCTCA GAGAAAGACT TCGTGGTTCG GCAGAGAAGG 
GGCAAGGAGA CCTTAAGGAG CAGTCCTCAC AAAAAGGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_006517707.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517770.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X19, mRNA.

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CloneID	OMu49083
Clone ID Related Accession (Same CDS sequence)	XM_006517707.3
Accession Version	XM_006517707.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517707.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC ATGTATGGAC TTCGTGGGGA AGTCCCAGTT GCCGAACAAG CAACCGGAAG 
AGTTTGATAG GCAATGGCCA GTCTCCAGCA CTGCCTCGAC CACACTCACC TCTCTCTGCT 
CATGCAGGAA ATAGCCCTCA AGACAGTCCA AGGAATTTCT CCCCCAGTGC CTCAGCCCAT 
TTCTCATTTG CGAGGAGAAA TGACAGAACG GATGGGCGCC GCTGGTCTCT GGCTTCTCTC 
CCTTCCTCAG GCTATGGGAC AAATACCCCA AGCTCCACCG TCTCCTCATC CTGTTCTTCC 
CAGGAGAAGT TGCACCAGCT ACCATACCAG CCAACCCCAG ATGAATTACA CTTCTTATCC 
AAACACTTCT GCACAACAGA AAGTATCGCC ACTGAGAACC GGTGCAGAAA CACACCCATG 
CGTCCACGTT CCCGGAGTCT CAGCCCTGGA CGGTCCCCCG CCTGCTGTGA CCATGAAATA 
ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CCACAGCTCA GATGGAAGAG 
CGTCTGAAGG AGATCATCAC CAGCTACTCT CCAGACCATG TTCTCCCCTT GGCAGATGGG 
GTACTTAGTT TCACTCACCA TCAGATCATT GAGCTGGCTC GGGACTGTTT GGATAAATCT 
CACCAGGGTC TCATCACGTC GAGATACTTT TTTGAGTTGC AGCACAAACT GGACAAGTTG 
CTCCAGGAGG CTCACGATCG CTCTGAAAGT GGAGAACTGG CATTTATCAA GCAACTAGTC 
CGAAAGATCC TAATTGTCAT TGCCCGCCCC GCTCGGTTAT TGGAGTGTTT GGAATTTGAT 
CCTGAAGAAT TTTATTACCT ATTGGAAGCT GCAGAAGGCC ATGCCAAAGA AGGCCAAGGA 
ATCAAAACTG ACATCCCTAG GTATATCATC AGCCAGCTGG GACTCAATAA GGACCCTTTA 
GAAGAAATGG CTCAGTTGGG GAATTACGAC AGTAGGACAG CAGAGACACC AGAGATGGAT 
GAGTCAGTGA GTAGCTCAAA TACTTCCCTG AGACTTCGAA GGAAACCCCG AGAGAGTGAT 
TTTGAAACAA TTAAATTGAT CAGCAATGGA GCCTACGGGG CAGTCTACTT TGTTCGGCAC 
AAAGAGTCCC GCCAGAGGTT TGCCATGAAG AAGATCAACA AGCAGAACCT CATCCTTCGG 
AACCAGATCC AGCAGGCCTT CGTGGAGCGA GACATCCTGA CTTTCGCAGA GAACCCCTTT 
GTGGTCAGCA TGTATTGCTC CTTTGAAACG AGGCGTCACT TATGCATGGT CATGGAGTAT 
GTAGAAGGGG GAGACTGTGC GACCCTAATG AAAAACATGG GACCTCTCCC AGTTGATATG 
GCCAGAATGT ATTTCGCCGA GACCGTCTTG GCCTTGGAGT ACCTGCATAA TTACGGAATC 
GTACACAGGG ACTTGAAGCC AGACAACCTG TTGGTCACCT CCATGGGGCA CATAAAACTG 
ACTGACTTCG GCTTGTCTAA GGTGGGATTA ATGAGCATGA CCACCAATCT CTATGAAGGC 
CACATAGAGA AGGACGCTCG AGAGTTCTTA GATAAACAGG TCTGTGGTAC ACCTGAGTAC 
ATTGCCCCCG AGGTGATTCT GAGACAGGGC TACGGGAAAC CCGTGGACTG GTGGGCCATG 
GGCATTATCC TCTATGAATT TCTGGTCGGA TGTGTGCCTT TCTTTGGGGA CACTCCAGAA 
GAGCTATTTG GACAAGTCAT CAGTGATGAA ATCAACTGGC CTGAAAAGGA CGAGGCCCCT 
CCTCCAGACG CTCAGGAGCT GATTACCTTG CTCCTCAGGC AGAATCCGCT GGAGAGGCTG 
GGAACAGGTG GAGCCTATGA AGTGAAGCAG CATCGTTTCT TCCGCTCCTT AGACTGGAAC 
AGTTTGCTGA GACAGAAGGC GGAATTTATT CCCCAACTGG AATCGGAGGA TGACACAAGT 
TATTTTGATA CTCGGTCAGA GAAGTATCAT CACATGGAGA CGGAGGAGGA GGACGATACA 
AACGATGAGG ACTTCACCGT GGAGATAAGG CAGTTTTCTT CCTGTTCACA CAGGTTTTCA 
AAAGTTTTCA GCAGTATAGA TCGCATAACT CAAAATTCAG GAGAAGACAA AGATGACTCT 
GAGGACAAGA CCAAAAGCAC AACGTTGCCA TCCACAGAGA CACTCAGCTG GAGTTCCGAA 
TACTCTGAAA TGCAACAGTT ATCGACCTCC AACTCTTCAG ATACTGAAAG CAACAGGTGC 
AAACTCAGCT CTGGCTTGCT CCCCAAGCTG GCTATTTCGA CAGATGGGGA ACAAGATGAG 
GCTGTCCCTT GCTCTGGAGA CCCCAGAGAG GAGCCAGAGA AACCTGTCCC TCCCTCTGAG 
GAGTGTACTC AGGAGGAGCC CGAGGTCACC ACCCCAGCCA GCACCATCAG CAGTTCCACA 
CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGGCGAAG CGAGTGTGTG 
GACAGTACAG ATAATTCCTC AAAGCCATCC AGTGAACCCA CTTCTCACGT GGCTCGACAG 
CGCTTAGAAA GCACAGAGAA AAAGAAAATT TCTGGGAAAG TCACAAAGTC CCTCTCGGCC 
AGTGCTCTGT CCCTCATGAT CCCAGGAGAT ATGTTCGCTG TATCTCCATT GGGAAGCCCA 
ATGTCCCCAC ACTCCCTGTC TTCAGACCCT TCTTCTTCAC GGGATTCCTC TCCCAGCCGA 
GACTCTTCTG CAGCATCTGC CAGTCCGCAT CAGCCCATTG TCATCCACAG CTCAGGCAAG 
AACTATGGGT TCACCATCCG TGCTATCCGC GTGTACGTGG GGGACAGTGA CATCTACACA 
GTGCACCATA TCGTCTGGAA CGTAGAAGAA GGAAGTCCCG CATACCAGGC AGGACTGAAG 
GCCGGGGATC TGATCACACA CATCAACGGA GAGCCGGTGC ACGGCCTCGT CCACACGGAA 
GTTATCGAGC TCCTGCTGAA GAGTGGGAAT AAGGTGTCTA TCACCACTAC TCCATTTGAA 
AACACATCAA TCAAAACGGG ACCAGCCAGG AGAAACAGTT ACAAGGGCCG GATGGTGAGA 
CGAAGCAAGA AGTCCAAGAA GAAGGAGAGT CTAGAAAGGA GGAGATCTCT CTTCAAGAAG 
CTGGCCAAGC AGCCTTCTCC TTTGCTCCAC ACCAGCCGAA GTTTCTCCTG CTTAAACCGG 
TCCCTGTCAT CTGGAGAGAG CCTCCCGGGT TCCCCAACTC ACAGCTTGTC CCCGAGGTCT 
CCAACACCCA GTTATCGTTC TACTCCCGAT TTTCCGTCAG GTACAAATTC CTCCCAGAGC 
AGCTCCCCAA GTTCAAGTGC CCCCAATTCT CCAGCAGGTT CAGGGCACAT CCGGCCCAGC 
ACCCTCCATG GCCTGGCTCC CAAACTCAGC GGGCAGCGAT ACCGCTCTGG AAGACGGAAG 
TCGGCTGGCA GCATCCCTCT CTCCCCGCTG GCCAGGACAC CCTCTCCCAC TCCACAGCCT 
ACCTCTCCTC AGCGTTCACC ATCCCCACTG TTGGGACACT CACTGGGCAA TGCCAAGATC 
ACTCAGGCCT TTCCTAGCAA GATGCACTCT CCCCCCACAA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CAGAGCCGCC CCGCTCCCCA CTGCTGAAAC GGGTGCAGTC GGAGGAAAAG 
TTGTCACCCT CCTATGGCAG TGACAAGAAG CTTCTGTGCT CCCGCAAGCA TAGCCTAGAG 
GTGACACAAG AGGAGGTACA GAGGGAGCAG TGTCAGCGGG AAGTGACACT GCAGAGCCTG 
GAAGAGAATG TGTGTGACGC TCCTTCCCTC AGTCGGGCCA GGCCAGTGGA GCAAGGCTGT 
CTGAAACGCC CCGTGTCCCG GAAGGTGGGC AGGCAAGAGT CTGTGGATGA CCTGGACCGG 
GACAAGCTGA AAGCCAAGGT TGTCGTAAAG AAACCAGAAG AGAAACATGA ATCGCACCAG 
AAACCTCACA GCCTTGGTGG TGATTCGGAA AGCTATGCTC TCTTCAGGCT AGAGGAGAGA 
GAGAAAAAAG TGTACTCCAA GGGGTTGGAA AGGTCAGGCC ATTTTGAAAA CACATCAGCA 
GAGTTGCCTT CTGTGGGCAG CCTGCTGAAG GACACTCTTC ACAAGCAGGC CAGCGTGAGG 
GCCAGCGAGG GGGTGACCTC AGACGGGGCA GCTTGCAGCC TGACACCAGG GGAGCACAGC 
CAGTCTCTAG GTGACTTTAA GCGGGCCTCA GCTTCTGGCA TTCTCCATGA TAGTGTGTGC 
CCCATCTCTG ATAGGCCTGC TCCTGGAAAG GTTGAATACT CGGAGAAGGC CTCTCAGGCC 
AAAGAGCTCC TTCGAAGTGA AAAACTAGAC AGCAAGCTGG CCAATATTGA TTACCTCAGA 
AAGAAAATGT CATTGGATGA CAAAGATGAC AGCCACTGTG CCATCCTGAA ACCCAAGATA 
ACATCTAGCG CCCATGAATG TCTGCCAGGG AACCCCATAC GGCCCATGGC AGGGCAACAA 
GAGACCCCGC CAGCCTCTGA AAACCGGGCA TTCATCAACA GTACCCACAC ACCTCAGATG 
AGTGCAGTTT CCTTTGTTCC TCTCAAAGCC TTAGCTGGCC GGGTAGAGAA CGGAGGGGAG 
AAAGCAGGCT TAGCTGCTCC CGAGTCCCCT GTCAGGAAGA GCCCCTCCGA GTATAAGCTA 
GAGGGCAGGT CAGTTTCATG TCTCAAGCCG ATCGAGGGCA CACTGGACAT TGCTCTCCTG 
TCTGGACCTC ACGCCTCCAA AACAGAGTTG CTTTCCCCAG AGCCTGCACA GAGTCCCAGC 
CCAGGCATCA ACGTGGGACC ATGTGTGCCA CTAGCTCTTC CTGGGAGCAG TGGGAAAAAG 
GGAGACTCCA CCAGCCTGAG AGAGCCTTCC TCAGCCAACT TAAAAGTAAA TAAATCTTAT 
CTGCTGGAGC CTCGGTTCCT ACCCCCGAGC CGGGCTCTCC AGGACTCTCT CGCAGCCTCT 
GGGCCAGAAC CGAAGTCAAA GCCGGAAAGG AAGCTCATTC ATCCTTCTGC CAGGAGCCCA 
GCAACTGTCA CAGAGAGCAA TCTTCAGCAG AAAGAGGGTG GTCCCGCCAC ACACCAAGAC 
CGCTCCACTG ACACCAGGAA CCTCCCTGGC CCAGGGCAGA CCCTACACAA TGTGGACCTA 
CCCAGGCTGT GTACACGTGC CCCACTCCCA CCGGAAGGGA CGCCCGCAAA GGAGAAGCCA 
TGTCTGAAGG AACCCTCTGC CAAGGTGAAA AGCGAGTGGT CTGCCGTGAG GGATGACGGA 
CACAGAGATC CCTGTGCGAA GCTGTGCCCG GCAGAGACTG GTAAAGCCAG CGACAGTTCC 
AAACCCCTGC CTTCCGGGGG GAGGACCCAA CCCGATTTCT ACAAGCAGAC CCAGACTTCG 
GAGAAAGCAT GGGCGCATGC AAAAACAAAC CACAAAGATA GCCAAGATGA GGTGAAGTCC 
CTGGCCAGGG AGGACTCAGC TTCACTTTTA TATGAAAAGG AGATAGGCCG GGCACGAAAA 
GGTCCTGAAC CCAAACCGGA AGTTCCTGCT ACCCGGTGCC CTCCTCAGCC ACCAGGAATT 
GAGGGTGAGA AGCGAGAAAA GCTCTCCGCT GCCCCCTCTT TGCAGAAACA GGCTCCCAAA 
GAGCCAGACA GGAAGGAACA GACTTCGCAA AGGCCTGGAG GTAGTGGCCC TCAACAACCC 
CCACCCACCA AAGAGCTGTC TAACTCAGCA TCCTGGCAGC ACGGCAGTTC TCCGAGTCAC 
ACTTTAAAGA AGGAGCCCGG GACCAAAGCT GCCGCTGCAG AACCAAGCAC CAGCCTTCAT 
GACACTCCCC GATCTGCTAC AGCCACCACC ACTGCCATTG CCACCACCAC CACTACCACC 
AGTGCCGGGC ACAGTGACTG CAGTAGCCAT AAGGCCCGGC CTGGCCCTGA CCCCAGCCCT 
TCAAAGTCTA AGCACCAAGA CAGGTCCCTC TCCTCACAGA AGCTGAGTGC TGGCTCTGCA 
AAAGGCAAAG AGCCTGTCAC TCAACCCCTG GGTGGTTCCA TCAGAGAAGG CAAGGGTGGC 
AGCAAGGGTC CAGTGGACAC ATTTTCTGCT GTCCTGACCA CCCAGGGCAA AGCAAGTGAT 
GTGCTTGTGC AGGGAGAAGG TCGGGTCTCA ATCATTGTCC ACACTGAAGA GTGTCCTCTC 
GATGCCAAAC TGAAAAACAC CAATGGAGGG TGTCCCCCAG AGATGCAGGC GAAGCATCCA 
CCCAGACAAG GACATCTCAG TGAAGCAGCA GACCAGAAGC CACTCATTGC TGGTGAGAAG 
CAAAGCCCGT CTCCAAAGCA TCCCAAACCA TCCACTGTGA AAGATTACCC CAGTCTGTGC 
AGACAGACAG ACAGAAGCCC AAGCCATCAG GCTACCACTG GGGACAGGAA GGCAGAAGGA 
AAGAAATGCA CAGACGCACT TTATGTCGCA GCCCCAGAGG GCTACAAGCC AGAGGCCAGC 
CCTTCTCTCC ACCACGGCGA GACCGGACTC AGAGGCTCAG AGAGGCCACC CATGGGCATG 
GGGAAGGGCT TCTCTGAGCC CAAGGGGAAA GGGCCAGGTC CCCAGAAGTC ACTGGCTGAA 
ACAGGCAAGC CCAGCGGTAT GAAAAGGTCA CCCTCTGCCA CCGTGCAGAG CTCTCTCCGC 
TCAGCTGCCC CCCCAGAAAA GTCTCTGAGT TACTCAGCCA GCTTTCCCGA GGCCCAGCCT 
GGAGTGCGAG AGGTCCCTGC AGCCAACAGC AGCCCCTCAT CTGCCAAGGC TACAGGGGGG 
ACCTCAGAGT TCCCAGCCCC CAGCAGCAGG GACCACAGGA AGCTTCAGTC TGGAGGAGAC 
GGCCGAAGCC AAATGATAAA GAGTGACTCT CTGCCCTCCT TCCGCCTCTC CACCTCTGCT 
CTGGAGTCAC ATTTCCAGGA TCCACAGGTG CCCATCGCAT CAGGCCACCG AGGCAGGGCA 
CTGTCAGTAA CTGCTGCCAC AGGAGAACCC AAAGGGAGAG AGCTCGCCCA GCCTCCCCCA 
GTCAGGAAAC AGAATGCGTG CAGAGAGGCG ACCAGAGCAC CCCCAGCCCC AAGCACAGAT 
CGCTCCCTCC CTCTTTCCTC AGAGAAAGAC TTCGTGGTTC GGCAGAGAAG GGGCAAGGAG 
ACCTTAAGGA GCAGTCCTCA CAAAAAGGCC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517770.1
CDS	16..7251
Translation	MAQHVWTSWGSPSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_006517707.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517707.3

ORF Insert Sequence:

ATGGCCCAGC ATGTATGGAC TTCGTGGGGA AGTCCCAGTT GCCGAACAAG CAACCGGAAG 
AGTTTGATAG GCAATGGCCA GTCTCCAGCA CTGCCTCGAC CACACTCACC TCTCTCTGCT 
CATGCAGGAA ATAGCCCTCA AGACAGTCCA AGGAATTTCT CCCCCAGTGC CTCAGCCCAT 
TTCTCATTTG CGAGGAGAAA TGACAGAACG GATGGGCGCC GCTGGTCTCT GGCTTCTCTC 
CCTTCCTCAG GCTATGGGAC AAATACCCCA AGCTCCACCG TCTCCTCATC CTGTTCTTCC 
CAGGAGAAGT TGCACCAGCT ACCATACCAG CCAACCCCAG ATGAATTACA CTTCTTATCC 
AAACACTTCT GCACAACAGA AAGTATCGCC ACTGAGAACC GGTGCAGAAA CACACCCATG 
CGTCCACGTT CCCGGAGTCT CAGCCCTGGA CGGTCCCCCG CCTGCTGTGA CCATGAAATA 
ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CCACAGCTCA GATGGAAGAG 
CGTCTGAAGG AGATCATCAC CAGCTACTCT CCAGACCATG TTCTCCCCTT GGCAGATGGG 
GTACTTAGTT TCACTCACCA TCAGATCATT GAGCTGGCTC GGGACTGTTT GGATAAATCT 
CACCAGGGTC TCATCACGTC GAGATACTTT TTTGAGTTGC AGCACAAACT GGACAAGTTG 
CTCCAGGAGG CTCACGATCG CTCTGAAAGT GGAGAACTGG CATTTATCAA GCAACTAGTC 
CGAAAGATCC TAATTGTCAT TGCCCGCCCC GCTCGGTTAT TGGAGTGTTT GGAATTTGAT 
CCTGAAGAAT TTTATTACCT ATTGGAAGCT GCAGAAGGCC ATGCCAAAGA AGGCCAAGGA 
ATCAAAACTG ACATCCCTAG GTATATCATC AGCCAGCTGG GACTCAATAA GGACCCTTTA 
GAAGAAATGG CTCAGTTGGG GAATTACGAC AGTAGGACAG CAGAGACACC AGAGATGGAT 
GAGTCAGTGA GTAGCTCAAA TACTTCCCTG AGACTTCGAA GGAAACCCCG AGAGAGTGAT 
TTTGAAACAA TTAAATTGAT CAGCAATGGA GCCTACGGGG CAGTCTACTT TGTTCGGCAC 
AAAGAGTCCC GCCAGAGGTT TGCCATGAAG AAGATCAACA AGCAGAACCT CATCCTTCGG 
AACCAGATCC AGCAGGCCTT CGTGGAGCGA GACATCCTGA CTTTCGCAGA GAACCCCTTT 
GTGGTCAGCA TGTATTGCTC CTTTGAAACG AGGCGTCACT TATGCATGGT CATGGAGTAT 
GTAGAAGGGG GAGACTGTGC GACCCTAATG AAAAACATGG GACCTCTCCC AGTTGATATG 
GCCAGAATGT ATTTCGCCGA GACCGTCTTG GCCTTGGAGT ACCTGCATAA TTACGGAATC 
GTACACAGGG ACTTGAAGCC AGACAACCTG TTGGTCACCT CCATGGGGCA CATAAAACTG 
ACTGACTTCG GCTTGTCTAA GGTGGGATTA ATGAGCATGA CCACCAATCT CTATGAAGGC 
CACATAGAGA AGGACGCTCG AGAGTTCTTA GATAAACAGG TCTGTGGTAC ACCTGAGTAC 
ATTGCCCCCG AGGTGATTCT GAGACAGGGC TACGGGAAAC CCGTGGACTG GTGGGCCATG 
GGCATTATCC TCTATGAATT TCTGGTCGGA TGTGTGCCTT TCTTTGGGGA CACTCCAGAA 
GAGCTATTTG GACAAGTCAT CAGTGATGAA ATCAACTGGC CTGAAAAGGA CGAGGCCCCT 
CCTCCAGACG CTCAGGAGCT GATTACCTTG CTCCTCAGGC AGAATCCGCT GGAGAGGCTG 
GGAACAGGTG GAGCCTATGA AGTGAAGCAG CATCGTTTCT TCCGCTCCTT AGACTGGAAC 
AGTTTGCTGA GACAGAAGGC GGAATTTATT CCCCAACTGG AATCGGAGGA TGACACAAGT 
TATTTTGATA CTCGGTCAGA GAAGTATCAT CACATGGAGA CGGAGGAGGA GGACGATACA 
AACGATGAGG ACTTCACCGT GGAGATAAGG CAGTTTTCTT CCTGTTCACA CAGGTTTTCA 
AAAGTTTTCA GCAGTATAGA TCGCATAACT CAAAATTCAG GAGAAGACAA AGATGACTCT 
GAGGACAAGA CCAAAAGCAC AACGTTGCCA TCCACAGAGA CACTCAGCTG GAGTTCCGAA 
TACTCTGAAA TGCAACAGTT ATCGACCTCC AACTCTTCAG ATACTGAAAG CAACAGGTGC 
AAACTCAGCT CTGGCTTGCT CCCCAAGCTG GCTATTTCGA CAGATGGGGA ACAAGATGAG 
GCTGTCCCTT GCTCTGGAGA CCCCAGAGAG GAGCCAGAGA AACCTGTCCC TCCCTCTGAG 
GAGTGTACTC AGGAGGAGCC CGAGGTCACC ACCCCAGCCA GCACCATCAG CAGTTCCACA 
CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGGCGAAG CGAGTGTGTG 
GACAGTACAG ATAATTCCTC AAAGCCATCC AGTGAACCCA CTTCTCACGT GGCTCGACAG 
CGCTTAGAAA GCACAGAGAA AAAGAAAATT TCTGGGAAAG TCACAAAGTC CCTCTCGGCC 
AGTGCTCTGT CCCTCATGAT CCCAGGAGAT ATGTTCGCTG TATCTCCATT GGGAAGCCCA 
ATGTCCCCAC ACTCCCTGTC TTCAGACCCT TCTTCTTCAC GGGATTCCTC TCCCAGCCGA 
GACTCTTCTG CAGCATCTGC CAGTCCGCAT CAGCCCATTG TCATCCACAG CTCAGGCAAG 
AACTATGGGT TCACCATCCG TGCTATCCGC GTGTACGTGG GGGACAGTGA CATCTACACA 
GTGCACCATA TCGTCTGGAA CGTAGAAGAA GGAAGTCCCG CATACCAGGC AGGACTGAAG 
GCCGGGGATC TGATCACACA CATCAACGGA GAGCCGGTGC ACGGCCTCGT CCACACGGAA 
GTTATCGAGC TCCTGCTGAA GAGTGGGAAT AAGGTGTCTA TCACCACTAC TCCATTTGAA 
AACACATCAA TCAAAACGGG ACCAGCCAGG AGAAACAGTT ACAAGGGCCG GATGGTGAGA 
CGAAGCAAGA AGTCCAAGAA GAAGGAGAGT CTAGAAAGGA GGAGATCTCT CTTCAAGAAG 
CTGGCCAAGC AGCCTTCTCC TTTGCTCCAC ACCAGCCGAA GTTTCTCCTG CTTAAACCGG 
TCCCTGTCAT CTGGAGAGAG CCTCCCGGGT TCCCCAACTC ACAGCTTGTC CCCGAGGTCT 
CCAACACCCA GTTATCGTTC TACTCCCGAT TTTCCGTCAG GTACAAATTC CTCCCAGAGC 
AGCTCCCCAA GTTCAAGTGC CCCCAATTCT CCAGCAGGTT CAGGGCACAT CCGGCCCAGC 
ACCCTCCATG GCCTGGCTCC CAAACTCAGC GGGCAGCGAT ACCGCTCTGG AAGACGGAAG 
TCGGCTGGCA GCATCCCTCT CTCCCCGCTG GCCAGGACAC CCTCTCCCAC TCCACAGCCT 
ACCTCTCCTC AGCGTTCACC ATCCCCACTG TTGGGACACT CACTGGGCAA TGCCAAGATC 
ACTCAGGCCT TTCCTAGCAA GATGCACTCT CCCCCCACAA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CAGAGCCGCC CCGCTCCCCA CTGCTGAAAC GGGTGCAGTC GGAGGAAAAG 
TTGTCACCCT CCTATGGCAG TGACAAGAAG CTTCTGTGCT CCCGCAAGCA TAGCCTAGAG 
GTGACACAAG AGGAGGTACA GAGGGAGCAG TGTCAGCGGG AAGTGACACT GCAGAGCCTG 
GAAGAGAATG TGTGTGACGC TCCTTCCCTC AGTCGGGCCA GGCCAGTGGA GCAAGGCTGT 
CTGAAACGCC CCGTGTCCCG GAAGGTGGGC AGGCAAGAGT CTGTGGATGA CCTGGACCGG 
GACAAGCTGA AAGCCAAGGT TGTCGTAAAG AAACCAGAAG AGAAACATGA ATCGCACCAG 
AAACCTCACA GCCTTGGTGG TGATTCGGAA AGCTATGCTC TCTTCAGGCT AGAGGAGAGA 
GAGAAAAAAG TGTACTCCAA GGGGTTGGAA AGGTCAGGCC ATTTTGAAAA CACATCAGCA 
GAGTTGCCTT CTGTGGGCAG CCTGCTGAAG GACACTCTTC ACAAGCAGGC CAGCGTGAGG 
GCCAGCGAGG GGGTGACCTC AGACGGGGCA GCTTGCAGCC TGACACCAGG GGAGCACAGC 
CAGTCTCTAG GTGACTTTAA GCGGGCCTCA GCTTCTGGCA TTCTCCATGA TAGTGTGTGC 
CCCATCTCTG ATAGGCCTGC TCCTGGAAAG GTTGAATACT CGGAGAAGGC CTCTCAGGCC 
AAAGAGCTCC TTCGAAGTGA AAAACTAGAC AGCAAGCTGG CCAATATTGA TTACCTCAGA 
AAGAAAATGT CATTGGATGA CAAAGATGAC AGCCACTGTG CCATCCTGAA ACCCAAGATA 
ACATCTAGCG CCCATGAATG TCTGCCAGGG AACCCCATAC GGCCCATGGC AGGGCAACAA 
GAGACCCCGC CAGCCTCTGA AAACCGGGCA TTCATCAACA GTACCCACAC ACCTCAGATG 
AGTGCAGTTT CCTTTGTTCC TCTCAAAGCC TTAGCTGGCC GGGTAGAGAA CGGAGGGGAG 
AAAGCAGGCT TAGCTGCTCC CGAGTCCCCT GTCAGGAAGA GCCCCTCCGA GTATAAGCTA 
GAGGGCAGGT CAGTTTCATG TCTCAAGCCG ATCGAGGGCA CACTGGACAT TGCTCTCCTG 
TCTGGACCTC ACGCCTCCAA AACAGAGTTG CTTTCCCCAG AGCCTGCACA GAGTCCCAGC 
CCAGGCATCA ACGTGGGACC ATGTGTGCCA CTAGCTCTTC CTGGGAGCAG TGGGAAAAAG 
GGAGACTCCA CCAGCCTGAG AGAGCCTTCC TCAGCCAACT TAAAAGTAAA TAAATCTTAT 
CTGCTGGAGC CTCGGTTCCT ACCCCCGAGC CGGGCTCTCC AGGACTCTCT CGCAGCCTCT 
GGGCCAGAAC CGAAGTCAAA GCCGGAAAGG AAGCTCATTC ATCCTTCTGC CAGGAGCCCA 
GCAACTGTCA CAGAGAGCAA TCTTCAGCAG AAAGAGGGTG GTCCCGCCAC ACACCAAGAC 
CGCTCCACTG ACACCAGGAA CCTCCCTGGC CCAGGGCAGA CCCTACACAA TGTGGACCTA 
CCCAGGCTGT GTACACGTGC CCCACTCCCA CCGGAAGGGA CGCCCGCAAA GGAGAAGCCA 
TGTCTGAAGG AACCCTCTGC CAAGGTGAAA AGCGAGTGGT CTGCCGTGAG GGATGACGGA 
CACAGAGATC CCTGTGCGAA GCTGTGCCCG GCAGAGACTG GTAAAGCCAG CGACAGTTCC 
AAACCCCTGC CTTCCGGGGG GAGGACCCAA CCCGATTTCT ACAAGCAGAC CCAGACTTCG 
GAGAAAGCAT GGGCGCATGC AAAAACAAAC CACAAAGATA GCCAAGATGA GGTGAAGTCC 
CTGGCCAGGG AGGACTCAGC TTCACTTTTA TATGAAAAGG AGATAGGCCG GGCACGAAAA 
GGTCCTGAAC CCAAACCGGA AGTTCCTGCT ACCCGGTGCC CTCCTCAGCC ACCAGGAATT 
GAGGGTGAGA AGCGAGAAAA GCTCTCCGCT GCCCCCTCTT TGCAGAAACA GGCTCCCAAA 
GAGCCAGACA GGAAGGAACA GACTTCGCAA AGGCCTGGAG GTAGTGGCCC TCAACAACCC 
CCACCCACCA AAGAGCTGTC TAACTCAGCA TCCTGGCAGC ACGGCAGTTC TCCGAGTCAC 
ACTTTAAAGA AGGAGCCCGG GACCAAAGCT GCCGCTGCAG AACCAAGCAC CAGCCTTCAT 
GACACTCCCC GATCTGCTAC AGCCACCACC ACTGCCATTG CCACCACCAC CACTACCACC 
AGTGCCGGGC ACAGTGACTG CAGTAGCCAT AAGGCCCGGC CTGGCCCTGA CCCCAGCCCT 
TCAAAGTCTA AGCACCAAGA CAGGTCCCTC TCCTCACAGA AGCTGAGTGC TGGCTCTGCA 
AAAGGCAAAG AGCCTGTCAC TCAACCCCTG GGTGGTTCCA TCAGAGAAGG CAAGGGTGGC 
AGCAAGGGTC CAGTGGACAC ATTTTCTGCT GTCCTGACCA CCCAGGGCAA AGCAAGTGAT 
GTGCTTGTGC AGGGAGAAGG TCGGGTCTCA ATCATTGTCC ACACTGAAGA GTGTCCTCTC 
GATGCCAAAC TGAAAAACAC CAATGGAGGG TGTCCCCCAG AGATGCAGGC GAAGCATCCA 
CCCAGACAAG GACATCTCAG TGAAGCAGCA GACCAGAAGC CACTCATTGC TGGTGAGAAG 
CAAAGCCCGT CTCCAAAGCA TCCCAAACCA TCCACTGTGA AAGATTACCC CAGTCTGTGC 
AGACAGACAG ACAGAAGCCC AAGCCATCAG GCTACCACTG GGGACAGGAA GGCAGAAGGA 
AAGAAATGCA CAGACGCACT TTATGTCGCA GCCCCAGAGG GCTACAAGCC AGAGGCCAGC 
CCTTCTCTCC ACCACGGCGA GACCGGACTC AGAGGCTCAG AGAGGCCACC CATGGGCATG 
GGGAAGGGCT TCTCTGAGCC CAAGGGGAAA GGGCCAGGTC CCCAGAAGTC ACTGGCTGAA 
ACAGGCAAGC CCAGCGGTAT GAAAAGGTCA CCCTCTGCCA CCGTGCAGAG CTCTCTCCGC 
TCAGCTGCCC CCCCAGAAAA GTCTCTGAGT TACTCAGCCA GCTTTCCCGA GGCCCAGCCT 
GGAGTGCGAG AGGTCCCTGC AGCCAACAGC AGCCCCTCAT CTGCCAAGGC TACAGGGGGG 
ACCTCAGAGT TCCCAGCCCC CAGCAGCAGG GACCACAGGA AGCTTCAGTC TGGAGGAGAC 
GGCCGAAGCC AAATGATAAA GAGTGACTCT CTGCCCTCCT TCCGCCTCTC CACCTCTGCT 
CTGGAGTCAC ATTTCCAGGA TCCACAGGTG CCCATCGCAT CAGGCCACCG AGGCAGGGCA 
CTGTCAGTAA CTGCTGCCAC AGGAGAACCC AAAGGGAGAG AGCTCGCCCA GCCTCCCCCA 
GTCAGGAAAC AGAATGCGTG CAGAGAGGCG ACCAGAGCAC CCCCAGCCCC AAGCACAGAT 
CGCTCCCTCC CTCTTTCCTC AGAGAAAGAC TTCGTGGTTC GGCAGAGAAG GGGCAAGGAG 
ACCTTAAGGA GCAGTCCTCA CAAAAAGGCC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( NM_175171.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780380.2 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), mRNA.

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CloneID	OMu04956
Clone ID Related Accession (Same CDS sequence)	NM_175171.3
Accession Version	NM_175171.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124387.5, AC165290.2, AC167975.3, AC114827.4, AC112791.3 and AC239606.3. On or before Sep 10, 2009 this sequence version replaced XM_915307.3, XM_283179.6, NM_175171.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK090136.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGAGA AAGTTTCCGA GGCGCCTGAG CCCGTGCCCC GGGGCTGCAG CGGACACGGC 
GCCCGGACCC TAGTCTCTTC GGCGGCAGCC GTGTCCTCGG AGGGCGCTTC CTCAGCGGAG 
TCATCCTCTG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CCAGCCGCTT CTCCTGCAGG 
TCGCAGCCGC CGCGGCCGCC GGGCGGCGCC CTGGGAACCC GGCTACCCGC CGCGTGGGCT 
CCCGCGCGCG TGGCTCTGGA GCGTGGAGTC CCTACCCTGC CGCTGCCGCA CCCGGGAGGA 
GCGGTGCTGC CGGTGCCCCA GGTCAGCAGC GCATCCCAAG AGGAGCAGGA TGAAGAGCTT 
GACCACATAC TGTCTCCGCC ACCCATGCCG TTTCGGAAAT GCAGCAACCC AGATGTGGCC 
TGCGGCCTCG GAAAATCACT GAAGTACAAG AGACAGCTTA GTGAGGATGG GAAGCAGCTG 
CGGCGGGGGA GCCTGGGAGG AGCCCTCACA GGGAGGTACC TCCTTCCAAA CCCGGTAGCA 
GGACAGGCCT GGCCTGCTTC GGCGGAGACG TCCAACCTCG TGCGCATGCG CAGCCAGGCC 
CTTGGCCAAT CGGCTCCCTC GCTCACAGCC AGCTTGAAGG AGCTGAGCCT CCCCCGAAGA 
GGAAGTCTTT GCCGAACAAG CAACCGGAAG AGTTTGATAG GCAATGGCCA GTCTCCAGCA 
CTGCCTCGAC CACACTCACC TCTCTCTGCT CATGCAGGAA ATAGCCCTCA AGACAGTCCA 
AGGAATTTCT CCCCCAGTGC CTCAGCCCAT TTCTCATTTG CGAGGAGAAC GGATGGGCGC 
CGCTGGTCTC TGGCTTCTCT CCCTTCCTCA GGCTATGGGA CAAATACCCC AAGCTCCACC 
GTCTCCTCAT CCTGTTCTTC CCAGGAGAAG TTGCACCAGC TACCATACCA GCCAACCCCA 
GATGAATTAC ACTTCTTATC CAAACACTTC TGCACAACAG AAAGTATCGC CACTGAGAAC 
CGGTGCAGAA ACACACCCAT GCGTCCACGT TCCCGGAGTC TCAGCCCTGG ACGGTCCCCC 
GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG 
GCCACAGCTC AGATGGAAGA GCGTCTGAAG GAGATCATCA CCAGCTACTC TCCAGACCAT 
GTTCTCCCCT TGGCAGATGG GGTACTTAGT TTCACTCACC ATCAGATCAT TGAGCTGGCT 
CGGGACTGTT TGGATAAATC TCACCAGGGT CTCATCACGT CGAGATACTT TTTTGAGTTG 
CAGCACAAAC TGGACAAGTT GCTCCAGGAG GCTCACGATC GCTCTGAAAG TGGAGAACTG 
GCATTTATCA AGCAACTAGT CCGAAAGATC CTAATTGTCA TTGCCCGCCC CGCTCGGTTA 
TTGGAGTGTT TGGAATTTGA TCCTGAAGAA TTTTATTACC TATTGGAAGC TGCAGAAGGC 
CATGCCAAAG AAGGCCAAGG AATCAAAACT GACATCCCTA GGTATATCAT CAGCCAGCTG 
GGACTCAATA AGGACCCTTT AGAAGAAATG GCTCAGTTGG GGAATTACGA CAGTAGGACA 
GCAGAGACAC CAGAGATGGA TGAGTCAGTG AGTAGCTCAA ATACTTCCCT GAGACTTCGA 
AGGAAACCCC GAGAGAGTGA TTTTGAAACA ATTAAATTGA TCAGCAATGG AGCCTACGGG 
GCAGTCTACT TTGTTCGGCA CAAAGAGTCC CGCCAGAGGT TTGCCATGAA GAAGATCAAC 
AAGCAGAACC TCATCCTTCG GAACCAGATC CAGCAGGCCT TCGTGGAGCG AGACATCCTG 
ACTTTCGCAG AGAACCCCTT TGTGGTCAGC ATGTATTGCT CCTTTGAAAC GAGGCGTCAC 
TTATGCATGG TCATGGAGTA TGTAGAAGGG GGAGACTGTG CGACCCTAAT GAAAAACATG 
GGACCTCTCC CAGTTGATAT GGCCAGAATG TATTTCGCCG AGACCGTCTT GGCCTTGGAG 
TACCTGCATA ATTACGGAAT CGTACACAGG GACTTGAAGC CAGACAACCT GTTGGTCACC 
TCCATGGGGC ACATAAAACT GACTGACTTC GGCTTGTCTA AGGTGGGATT AATGAGCATG 
ACCACCAATC TCTATGAAGG CCACATAGAG AAGGACGCTC GAGAGTTCTT AGATAAACAG 
GTCTGTGGTA CACCTGAGTA CATTGCCCCC GAGGTGATTC TGAGACAGGG CTACGGGAAA 
CCCGTGGACT GGTGGGCCAT GGGCATTATC CTCTATGAAT TTCTGGTCGG ATGTGTGCCT 
TTCTTTGGGG ACACTCCAGA AGAGCTATTT GGACAAGTCA TCAGTGATGA AATCAACTGG 
CCTGAAAAGG ACGAGGCCCC TCCTCCAGAC GCTCAGGAGC TGATTACCTT GCTCCTCAGG 
CAGAATCCGC TGGAGAGGCT GGGAACAGGT GGAGCCTATG AAGTGAAGCA GCATCGTTTC 
TTCCGCTCCT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CGGAATTTAT TCCCCAACTG 
GAATCGGAGG ATGACACAAG TTATTTTGAT ACTCGGTCAG AGAAGTATCA TCACATGGAG 
ACGGAGGAGG AGGACGATAC AAACGATGAG GACTTCACCG TGGAGATAAG GCAGTTTTCT 
TCCTGTTCAC ACAGGTTTTC AAAAGTTTTC AGCAGTATAG ATCGCATAAC TCAAAATTCA 
GGAGAAGACA AAGATGACTC TGAGGACAAG ACCAAAAGCA CAACGTTGCC ATCCACAGAG 
ACACTCAGCT GGAGTTCCGA ATACTCTGAA ATGCAACAGT TATCGACCTC CAACTCTTCA 
GATACTGAAA GCAACAGGTG CAAACTCAGC TCTGGCTTGC TCCCCAAGCT GGCTATTTCG 
ACAGATGGGG AACAAGATGA GGCTGTCCCT TGCTCTGGAG ACCCCAGAGA GGAGCCAGAG 
AAACCTGTCC CTCCCTCTGA GGAGTGTACT CAGGAGGAGC CCGAGGTCAC CACCCCAGCC 
AGCACCATCA GCAGTTCCAC ACTGTCAGTT GGCAGTTTTT CAGAGCACTT GGATCAGATA 
AATGGGCGAA GCGAGTGTGT GGACAGTACA GATAATTCCT CAAAGCCATC CAGTGAACCC 
ACTTCTCACG TGGCTCGACA GCGCTTAGAA AGCACAGAGA AAAAGAAAAT TTCTGGGAAA 
GTCACAAAGT CCCTCTCGGC CAGTGCTCTG TCCCTCATGA TCCCAGGAGA TATGTTCGCT 
GTATCTCCAT TGGGAAGCCC AATGTCCCCA CACTCCCTGT CTTCAGACCC TTCTTCTTCA 
CGGGATTCCT CTCCCAGCCG AGACTCTTCT GCAGCATCTG CCAGTCCGCA TCAGCCCATT 
GTCATCCACA GCTCAGGCAA GAACTATGGG TTCACCATCC GTGCTATCCG CGTGTACGTG 
GGGGACAGTG ACATCTACAC AGTGCACCAT ATCGTCTGGA ACGTAGAAGA AGGAAGTCCC 
GCATACCAGG CAGGACTGAA GGCCGGGGAT CTGATCACAC ACATCAACGG AGAGCCGGTG 
CACGGCCTCG TCCACACGGA AGTTATCGAG CTCCTGCTGA AGAGTGGGAA TAAGGTGTCT 
ATCACCACTA CTCCATTTGA AAACACATCA ATCAAAACGG GACCAGCCAG GAGAAACAGT 
TACAAGGGCC GGATGGTGAG ACGAAGCAAG AAGTCCAAGA AGAAGGAGAG TCTAGAAAGG 
AGGAGATCTC TCTTCAAGAA GCTGGCCAAG CAGCCTTCTC CTTTGCTCCA CACCAGCCGA 
AGTTTCTCCT GCTTAAACCG GTCCCTGTCA TCTGGAGAGA GCCTCCCGGG TTCCCCAACT 
CACAGCTTGT CCCCGAGGTC TCCAACACCC AGTTATCGTT CTACTCCCGA TTTTCCGTCA 
GGTACAAATT CCTCCCAGAG CAGCTCCCCA AGTTCAAGTG CCCCCAATTC TCCAGCAGGT 
TCAGGGCACA TCCGGCCCAG CACCCTCCAT GGCCTGGCTC CCAAACTCAG CGGGCAGCGA 
TACCGCTCTG GAAGACGGAA GTCGGCTGGC AGCATCCCTC TCTCCCCGCT GGCCAGGACA 
CCCTCTCCCA CTCCACAGCC TACCTCTCCT CAGCGTTCAC CATCCCCACT GTTGGGACAC 
TCACTGGGCA ATGCCAAGAT CACTCAGGCC TTTCCTAGCA AGATGCACTC TCCCCCCACA 
ATCGTCAGAC ACATCGTGAG GCCCAAGAGT GCAGAGCCGC CCCGCTCCCC ACTGCTGAAA 
CGGGTGCAGT CGGAGGAAAA GTTGTCACCC TCCTATGGCA GTGACAAGAA GCTTCTGTGC 
TCCCGCAAGC ATAGCCTAGA GGTGACACAA GAGGAGGTAC AGAGGGAGCA GTGTCAGCGG 
GAAGTGACAC TGCAGAGCCT GGAAGAGAAT GTGTGTGACG CTCCTTCCCT CAGTCGGGCC 
AGGCCAGTGG AGCAAGGCTG TCTGAAACGC CCCGTGTCCC GGAAGGTGGG CAGGCAAGAG 
TCTGTGGATG ACCTGGACCG GGACAAGCTG AAAGCCAAGG TTGTCGTAAA GAAACCAGAA 
GAGAAACATG AATCGCACCA GAAACCTCAC AGCCTTGGTG GTGATTCGGA AAGCTATGCT 
CTCTTCAGGC TAGAGGAGAG AGAGAAAAAA GTGTACTCCA AGGGGTTGGA AAGGTCAGGC 
CATTTTGAAA ACACATCAGC AGAGTTGCCT TCTGTGGGCA GCCTGCTGAA GGACACTCTT 
CACAAGCAGG CCAGCGTGAG GGCCAGCGAG GGGGTGACCT CAGACGGGGC AGCTTGCAGC 
CTGACACCAG GGGAGCACAG CCAGTCTCTA GGTGACTTTA AGCGGGCCTC AGCTTCTGGC 
ATTCTCCATG ATAGTGTGTG CCCCATCTCT GATAGGCCTG CTCCTGGAAA GGTTGAATAC 
TCGGAGAAGG CCTCTCAGGC CAAAGAGCTC CTTCGAAGTG AAAAACTAGA CAGCAAGCTG 
GCCAATATTG ATTACCTCAG AAAGAAAATG TCATTGGATG ACAAAGATGA CAGCCACTGT 
GCCATCCTGA AACCCAAGAT AACATCTAGC GCCCATGAAT GTCTGCCAGG GAACCCCATA 
CGGCCCATGG CAGGGCAACA AGAGACCCCG CCAGCCTCTG AAAACCGGGC ATTCATCAAC 
AGTACCCACA CACCTCAGAT GAGTGCAGTT TCCTTTGTTC CTCTCAAAGC CTTAGCTGGC 
CGGGTAGAGA ACGGAGGGGA GAAAGCAGGC TTAGCTGCTC CCGAGTCCCC TGTCAGGAAG 
AGCCCCTCCG AGTATAAGCT AGAGGGCAGG TCAGTTTCAT GTCTCAAGCC GATCGAGGGC 
ACACTGGACA TTGCTCTCCT GTCTGGACCT CACGCCTCCA AAACAGAGTT GCTTTCCCCA 
GAGCCTGCAC AGAGTCCCAG CCCAGGCATC AACGTGGGAC CATGTGTGCC ACTAGCTCTT 
CCTGGGAGCA GTGGGAAAAA GGGAGACTCC ACCAGCCTGA GAGAGCCTTC CTCAGCCAAC 
TTAAAAGTAA ATAAATCTTA TCTGCTGGAG CCTCGGTTCC TACCCCCGAG CCGGGCTCTC 
CAGGACTCTC TCGCAGCCTC TGGGCCAGAA CCGAAGTCAA AGCCGGAAAG GAAGCTCATT 
CATCCTTCTG CCAGGAGCCC AGCAACTGTC ACAGAGAGCA ATCTTCAGCA GAAAGAGGGT 
GGTCCCGCCA CACACCAAGA CCGCTCCACT GACACCAGGA ACCTCCCTGG CCCAGGGCAG 
ACCCTACACA ATGTGGACCT ACCCAGGCTG TGTACACGTG CCCCACTCCC ACCGGAAGGG 
ACGCCCGCAA AGGAGAAGCC ATGTCTGAAG GAACCCTCTG CCAAGGTGAA AAGCGAGTGG 
TCTGCCGTGA GGGATGACGG ACACAGAGAT CCCTGTGCGA AGCTGTGCCC GGCAGAGACT 
GGTAAAGCCA GCGACAGTTC CAAACCCCTG CCTTCCGGGG GGAGGACCCA ACCCGATTTC 
TACAAGCAGA CCCAGACTTC GGAGAAAGCA TGGGCGCATG CAAAAACAAA CCACAAAGAT 
AGCCAAGATG AGGTGAAGTC CCTGGCCAGG GAGGACTCAG CTTCACTTTT ATATGAAAAG 
GAGATAGGCC GGGCACGAAA AGGTCCTGAA CCCAAACCGG AAGTTCCTGC TACCCGGTGC 
CCTCCTCAGC CACCAGGAAT TGAGGGTGAG AAGCGAGAAA AGCTCTCCGC TGCCCCCTCT 
TTGCAGAAAC AGGCTCCCAA AGAGCCAGAC AGGAAGGAAC AGACTTCGCA AAGGCCTGGA 
GGTAGTGGCC CTCAACAACC CCCACCCACC AAAGAGCTGT CTAACTCAGC ATCCTGGCAG 
CACGGCAGTT CTCCGAGTCA CACTTTAAAG AAGGAGCCCG GGACCAAAGC TGCCGCTGCA 
GAACCAAGCA CCAGCCTTCA TGACACTCCC CGATCTGCTA CAGCCACCAC CACTGCCATT 
GCCACCACCA CCACTACCAC CAGTGCCGGG CACAGTGACT GCAGTAGCCA TAAGGCCCGG 
CCTGGCCCTG ACCCCAGCCC TTCAAAGTCT AAGCACCAAG ACAGGTCCCT CTCCTCACAG 
AAGCTGAGTG CTGGCTCTGC AAAAGGCAAA GAGCCTGTCA CTCAACCCCT GGGTGGTTCC 
ATCAGAGAAG GCAAGGGTGG CAGCAAGGGT CCAGTGGACA CATTTTCTGC TGTCCTGACC 
ACCCAGGGCA AAGCAAGTGA TGTGCTTGTG CAGGGAGAAG GTCGGGTCTC AATCATTGTC 
CACACTGAAG AGTGTCCTCT CGATGCCAAA CTGAAAAACA CCAATGGAGG GTGTCCCCCA 
GAGATGCAGG CGAAGCATCC ACCCAGACAA GGACATCTCA GTGAAGCAGC AGACCAGAAG 
CCACTCATTG CTGGTGAGAA GCAAAGCCCG TCTCCAAAGC ATCCCAAACC ATCCACTGTG 
AAAGATTACC CCAGTCTGTG CAGACAGACA GACAGAAGCC CAAGCCATCA GGCTACCACT 
GGGGACAGGA AGGCAGAAGG AAAGAAATGC ACAGACGCAC TTTATGTCGC AGCCCCAGAG 
GGCTACAAGC CAGAGGCCAG CCCTTCTCTC CACCACGGCG AGACCGGACT CAGAGGCTCA 
GAGAGGCCAC CCATGGGCAT GGGGAAGGGC TTCTCTGAGC CCAAGGGGAA AGGGCCAGGT 
CCCCAGAAGT CACTGGCTGA AACAGGCAAG CCCAGCGGTA TGAAAAGGTC ACCCTCTGCC 
ACCGTGCAGA GCTCTCTCCG CTCAGCTGCC CCCCCAGAAA AGTCTCTGAG TTACTCAGCC 
AGCTTTCCCG AGGCCCAGCC TGGAGTGCGA GAGGTCCCTG CAGCCAACAG CAGCCCCTCA 
TCTGCCAAGG CTACAGGGGG GACCTCAGAG TTCCCAGCCC CCAGCAGCAG GGACCACAGG 
AAGCTTCAGT CTGGAGGAGA CGGCCGAAGC CAAATGATAA AGAGTGACTC TCTGCCCTCC 
TTCCGCCTCT CCACCTCTGC TCTGGAGTCA CATTTCCAGG ATCCACAGGT GCCCATCGCA 
TCAGGCCACC GAGGCAGGGC ACTGTCAGTA ACTGCTGCCA CAGGAGAACC CAAAGGGAGA 
GAGCTCGCCC AGCCTCCCCC AGTCAGGAAA CAGAATGCGT GCAGAGAGGC GACCAGAGCA 
CCCCCAGCCC CAAGCACAGA TCGCTCCCTC CCTCTTTCCT CAGAGAAAGA CTTCGTGGTT 
CGGCAGAGAA GGGGCAAGGA GACCTTAAGG AGCAGTCCTC ACAAAAAGGC CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780380.2
CDS	301..8157
Translation	MGEKVSEAPEPVPRGCSGHGARTLVSSAAAVSSEGASSAESSSGSETLSEEGEPSRFSCRSQPPRPPGGALGTRLPAAWAPARVALERGVPTLPLPHPGGAVLPVPQVSSASQEEQDEELDHILSPPPMPFRKCSNPDVACGLGKSLKYKRQLSEDGKQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLKELSLPRRGSLCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	NM_175171.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175171.3

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCCGA GGCGCCTGAG CCCGTGCCCC GGGGCTGCAG CGGACACGGC 
GCCCGGACCC TAGTCTCTTC GGCGGCAGCC GTGTCCTCGG AGGGCGCTTC CTCAGCGGAG 
TCATCCTCTG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CCAGCCGCTT CTCCTGCAGG 
TCGCAGCCGC CGCGGCCGCC GGGCGGCGCC CTGGGAACCC GGCTACCCGC CGCGTGGGCT 
CCCGCGCGCG TGGCTCTGGA GCGTGGAGTC CCTACCCTGC CGCTGCCGCA CCCGGGAGGA 
GCGGTGCTGC CGGTGCCCCA GGTCAGCAGC GCATCCCAAG AGGAGCAGGA TGAAGAGCTT 
GACCACATAC TGTCTCCGCC ACCCATGCCG TTTCGGAAAT GCAGCAACCC AGATGTGGCC 
TGCGGCCTCG GAAAATCACT GAAGTACAAG AGACAGCTTA GTGAGGATGG GAAGCAGCTG 
CGGCGGGGGA GCCTGGGAGG AGCCCTCACA GGGAGGTACC TCCTTCCAAA CCCGGTAGCA 
GGACAGGCCT GGCCTGCTTC GGCGGAGACG TCCAACCTCG TGCGCATGCG CAGCCAGGCC 
CTTGGCCAAT CGGCTCCCTC GCTCACAGCC AGCTTGAAGG AGCTGAGCCT CCCCCGAAGA 
GGAAGTCTTT GCCGAACAAG CAACCGGAAG AGTTTGATAG GCAATGGCCA GTCTCCAGCA 
CTGCCTCGAC CACACTCACC TCTCTCTGCT CATGCAGGAA ATAGCCCTCA AGACAGTCCA 
AGGAATTTCT CCCCCAGTGC CTCAGCCCAT TTCTCATTTG CGAGGAGAAC GGATGGGCGC 
CGCTGGTCTC TGGCTTCTCT CCCTTCCTCA GGCTATGGGA CAAATACCCC AAGCTCCACC 
GTCTCCTCAT CCTGTTCTTC CCAGGAGAAG TTGCACCAGC TACCATACCA GCCAACCCCA 
GATGAATTAC ACTTCTTATC CAAACACTTC TGCACAACAG AAAGTATCGC CACTGAGAAC 
CGGTGCAGAA ACACACCCAT GCGTCCACGT TCCCGGAGTC TCAGCCCTGG ACGGTCCCCC 
GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG 
GCCACAGCTC AGATGGAAGA GCGTCTGAAG GAGATCATCA CCAGCTACTC TCCAGACCAT 
GTTCTCCCCT TGGCAGATGG GGTACTTAGT TTCACTCACC ATCAGATCAT TGAGCTGGCT 
CGGGACTGTT TGGATAAATC TCACCAGGGT CTCATCACGT CGAGATACTT TTTTGAGTTG 
CAGCACAAAC TGGACAAGTT GCTCCAGGAG GCTCACGATC GCTCTGAAAG TGGAGAACTG 
GCATTTATCA AGCAACTAGT CCGAAAGATC CTAATTGTCA TTGCCCGCCC CGCTCGGTTA 
TTGGAGTGTT TGGAATTTGA TCCTGAAGAA TTTTATTACC TATTGGAAGC TGCAGAAGGC 
CATGCCAAAG AAGGCCAAGG AATCAAAACT GACATCCCTA GGTATATCAT CAGCCAGCTG 
GGACTCAATA AGGACCCTTT AGAAGAAATG GCTCAGTTGG GGAATTACGA CAGTAGGACA 
GCAGAGACAC CAGAGATGGA TGAGTCAGTG AGTAGCTCAA ATACTTCCCT GAGACTTCGA 
AGGAAACCCC GAGAGAGTGA TTTTGAAACA ATTAAATTGA TCAGCAATGG AGCCTACGGG 
GCAGTCTACT TTGTTCGGCA CAAAGAGTCC CGCCAGAGGT TTGCCATGAA GAAGATCAAC 
AAGCAGAACC TCATCCTTCG GAACCAGATC CAGCAGGCCT TCGTGGAGCG AGACATCCTG 
ACTTTCGCAG AGAACCCCTT TGTGGTCAGC ATGTATTGCT CCTTTGAAAC GAGGCGTCAC 
TTATGCATGG TCATGGAGTA TGTAGAAGGG GGAGACTGTG CGACCCTAAT GAAAAACATG 
GGACCTCTCC CAGTTGATAT GGCCAGAATG TATTTCGCCG AGACCGTCTT GGCCTTGGAG 
TACCTGCATA ATTACGGAAT CGTACACAGG GACTTGAAGC CAGACAACCT GTTGGTCACC 
TCCATGGGGC ACATAAAACT GACTGACTTC GGCTTGTCTA AGGTGGGATT AATGAGCATG 
ACCACCAATC TCTATGAAGG CCACATAGAG AAGGACGCTC GAGAGTTCTT AGATAAACAG 
GTCTGTGGTA CACCTGAGTA CATTGCCCCC GAGGTGATTC TGAGACAGGG CTACGGGAAA 
CCCGTGGACT GGTGGGCCAT GGGCATTATC CTCTATGAAT TTCTGGTCGG ATGTGTGCCT 
TTCTTTGGGG ACACTCCAGA AGAGCTATTT GGACAAGTCA TCAGTGATGA AATCAACTGG 
CCTGAAAAGG ACGAGGCCCC TCCTCCAGAC GCTCAGGAGC TGATTACCTT GCTCCTCAGG 
CAGAATCCGC TGGAGAGGCT GGGAACAGGT GGAGCCTATG AAGTGAAGCA GCATCGTTTC 
TTCCGCTCCT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CGGAATTTAT TCCCCAACTG 
GAATCGGAGG ATGACACAAG TTATTTTGAT ACTCGGTCAG AGAAGTATCA TCACATGGAG 
ACGGAGGAGG AGGACGATAC AAACGATGAG GACTTCACCG TGGAGATAAG GCAGTTTTCT 
TCCTGTTCAC ACAGGTTTTC AAAAGTTTTC AGCAGTATAG ATCGCATAAC TCAAAATTCA 
GGAGAAGACA AAGATGACTC TGAGGACAAG ACCAAAAGCA CAACGTTGCC ATCCACAGAG 
ACACTCAGCT GGAGTTCCGA ATACTCTGAA ATGCAACAGT TATCGACCTC CAACTCTTCA 
GATACTGAAA GCAACAGGTG CAAACTCAGC TCTGGCTTGC TCCCCAAGCT GGCTATTTCG 
ACAGATGGGG AACAAGATGA GGCTGTCCCT TGCTCTGGAG ACCCCAGAGA GGAGCCAGAG 
AAACCTGTCC CTCCCTCTGA GGAGTGTACT CAGGAGGAGC CCGAGGTCAC CACCCCAGCC 
AGCACCATCA GCAGTTCCAC ACTGTCAGTT GGCAGTTTTT CAGAGCACTT GGATCAGATA 
AATGGGCGAA GCGAGTGTGT GGACAGTACA GATAATTCCT CAAAGCCATC CAGTGAACCC 
ACTTCTCACG TGGCTCGACA GCGCTTAGAA AGCACAGAGA AAAAGAAAAT TTCTGGGAAA 
GTCACAAAGT CCCTCTCGGC CAGTGCTCTG TCCCTCATGA TCCCAGGAGA TATGTTCGCT 
GTATCTCCAT TGGGAAGCCC AATGTCCCCA CACTCCCTGT CTTCAGACCC TTCTTCTTCA 
CGGGATTCCT CTCCCAGCCG AGACTCTTCT GCAGCATCTG CCAGTCCGCA TCAGCCCATT 
GTCATCCACA GCTCAGGCAA GAACTATGGG TTCACCATCC GTGCTATCCG CGTGTACGTG 
GGGGACAGTG ACATCTACAC AGTGCACCAT ATCGTCTGGA ACGTAGAAGA AGGAAGTCCC 
GCATACCAGG CAGGACTGAA GGCCGGGGAT CTGATCACAC ACATCAACGG AGAGCCGGTG 
CACGGCCTCG TCCACACGGA AGTTATCGAG CTCCTGCTGA AGAGTGGGAA TAAGGTGTCT 
ATCACCACTA CTCCATTTGA AAACACATCA ATCAAAACGG GACCAGCCAG GAGAAACAGT 
TACAAGGGCC GGATGGTGAG ACGAAGCAAG AAGTCCAAGA AGAAGGAGAG TCTAGAAAGG 
AGGAGATCTC TCTTCAAGAA GCTGGCCAAG CAGCCTTCTC CTTTGCTCCA CACCAGCCGA 
AGTTTCTCCT GCTTAAACCG GTCCCTGTCA TCTGGAGAGA GCCTCCCGGG TTCCCCAACT 
CACAGCTTGT CCCCGAGGTC TCCAACACCC AGTTATCGTT CTACTCCCGA TTTTCCGTCA 
GGTACAAATT CCTCCCAGAG CAGCTCCCCA AGTTCAAGTG CCCCCAATTC TCCAGCAGGT 
TCAGGGCACA TCCGGCCCAG CACCCTCCAT GGCCTGGCTC CCAAACTCAG CGGGCAGCGA 
TACCGCTCTG GAAGACGGAA GTCGGCTGGC AGCATCCCTC TCTCCCCGCT GGCCAGGACA 
CCCTCTCCCA CTCCACAGCC TACCTCTCCT CAGCGTTCAC CATCCCCACT GTTGGGACAC 
TCACTGGGCA ATGCCAAGAT CACTCAGGCC TTTCCTAGCA AGATGCACTC TCCCCCCACA 
ATCGTCAGAC ACATCGTGAG GCCCAAGAGT GCAGAGCCGC CCCGCTCCCC ACTGCTGAAA 
CGGGTGCAGT CGGAGGAAAA GTTGTCACCC TCCTATGGCA GTGACAAGAA GCTTCTGTGC 
TCCCGCAAGC ATAGCCTAGA GGTGACACAA GAGGAGGTAC AGAGGGAGCA GTGTCAGCGG 
GAAGTGACAC TGCAGAGCCT GGAAGAGAAT GTGTGTGACG CTCCTTCCCT CAGTCGGGCC 
AGGCCAGTGG AGCAAGGCTG TCTGAAACGC CCCGTGTCCC GGAAGGTGGG CAGGCAAGAG 
TCTGTGGATG ACCTGGACCG GGACAAGCTG AAAGCCAAGG TTGTCGTAAA GAAACCAGAA 
GAGAAACATG AATCGCACCA GAAACCTCAC AGCCTTGGTG GTGATTCGGA AAGCTATGCT 
CTCTTCAGGC TAGAGGAGAG AGAGAAAAAA GTGTACTCCA AGGGGTTGGA AAGGTCAGGC 
CATTTTGAAA ACACATCAGC AGAGTTGCCT TCTGTGGGCA GCCTGCTGAA GGACACTCTT 
CACAAGCAGG CCAGCGTGAG GGCCAGCGAG GGGGTGACCT CAGACGGGGC AGCTTGCAGC 
CTGACACCAG GGGAGCACAG CCAGTCTCTA GGTGACTTTA AGCGGGCCTC AGCTTCTGGC 
ATTCTCCATG ATAGTGTGTG CCCCATCTCT GATAGGCCTG CTCCTGGAAA GGTTGAATAC 
TCGGAGAAGG CCTCTCAGGC CAAAGAGCTC CTTCGAAGTG AAAAACTAGA CAGCAAGCTG 
GCCAATATTG ATTACCTCAG AAAGAAAATG TCATTGGATG ACAAAGATGA CAGCCACTGT 
GCCATCCTGA AACCCAAGAT AACATCTAGC GCCCATGAAT GTCTGCCAGG GAACCCCATA 
CGGCCCATGG CAGGGCAACA AGAGACCCCG CCAGCCTCTG AAAACCGGGC ATTCATCAAC 
AGTACCCACA CACCTCAGAT GAGTGCAGTT TCCTTTGTTC CTCTCAAAGC CTTAGCTGGC 
CGGGTAGAGA ACGGAGGGGA GAAAGCAGGC TTAGCTGCTC CCGAGTCCCC TGTCAGGAAG 
AGCCCCTCCG AGTATAAGCT AGAGGGCAGG TCAGTTTCAT GTCTCAAGCC GATCGAGGGC 
ACACTGGACA TTGCTCTCCT GTCTGGACCT CACGCCTCCA AAACAGAGTT GCTTTCCCCA 
GAGCCTGCAC AGAGTCCCAG CCCAGGCATC AACGTGGGAC CATGTGTGCC ACTAGCTCTT 
CCTGGGAGCA GTGGGAAAAA GGGAGACTCC ACCAGCCTGA GAGAGCCTTC CTCAGCCAAC 
TTAAAAGTAA ATAAATCTTA TCTGCTGGAG CCTCGGTTCC TACCCCCGAG CCGGGCTCTC 
CAGGACTCTC TCGCAGCCTC TGGGCCAGAA CCGAAGTCAA AGCCGGAAAG GAAGCTCATT 
CATCCTTCTG CCAGGAGCCC AGCAACTGTC ACAGAGAGCA ATCTTCAGCA GAAAGAGGGT 
GGTCCCGCCA CACACCAAGA CCGCTCCACT GACACCAGGA ACCTCCCTGG CCCAGGGCAG 
ACCCTACACA ATGTGGACCT ACCCAGGCTG TGTACACGTG CCCCACTCCC ACCGGAAGGG 
ACGCCCGCAA AGGAGAAGCC ATGTCTGAAG GAACCCTCTG CCAAGGTGAA AAGCGAGTGG 
TCTGCCGTGA GGGATGACGG ACACAGAGAT CCCTGTGCGA AGCTGTGCCC GGCAGAGACT 
GGTAAAGCCA GCGACAGTTC CAAACCCCTG CCTTCCGGGG GGAGGACCCA ACCCGATTTC 
TACAAGCAGA CCCAGACTTC GGAGAAAGCA TGGGCGCATG CAAAAACAAA CCACAAAGAT 
AGCCAAGATG AGGTGAAGTC CCTGGCCAGG GAGGACTCAG CTTCACTTTT ATATGAAAAG 
GAGATAGGCC GGGCACGAAA AGGTCCTGAA CCCAAACCGG AAGTTCCTGC TACCCGGTGC 
CCTCCTCAGC CACCAGGAAT TGAGGGTGAG AAGCGAGAAA AGCTCTCCGC TGCCCCCTCT 
TTGCAGAAAC AGGCTCCCAA AGAGCCAGAC AGGAAGGAAC AGACTTCGCA AAGGCCTGGA 
GGTAGTGGCC CTCAACAACC CCCACCCACC AAAGAGCTGT CTAACTCAGC ATCCTGGCAG 
CACGGCAGTT CTCCGAGTCA CACTTTAAAG AAGGAGCCCG GGACCAAAGC TGCCGCTGCA 
GAACCAAGCA CCAGCCTTCA TGACACTCCC CGATCTGCTA CAGCCACCAC CACTGCCATT 
GCCACCACCA CCACTACCAC CAGTGCCGGG CACAGTGACT GCAGTAGCCA TAAGGCCCGG 
CCTGGCCCTG ACCCCAGCCC TTCAAAGTCT AAGCACCAAG ACAGGTCCCT CTCCTCACAG 
AAGCTGAGTG CTGGCTCTGC AAAAGGCAAA GAGCCTGTCA CTCAACCCCT GGGTGGTTCC 
ATCAGAGAAG GCAAGGGTGG CAGCAAGGGT CCAGTGGACA CATTTTCTGC TGTCCTGACC 
ACCCAGGGCA AAGCAAGTGA TGTGCTTGTG CAGGGAGAAG GTCGGGTCTC AATCATTGTC 
CACACTGAAG AGTGTCCTCT CGATGCCAAA CTGAAAAACA CCAATGGAGG GTGTCCCCCA 
GAGATGCAGG CGAAGCATCC ACCCAGACAA GGACATCTCA GTGAAGCAGC AGACCAGAAG 
CCACTCATTG CTGGTGAGAA GCAAAGCCCG TCTCCAAAGC ATCCCAAACC ATCCACTGTG 
AAAGATTACC CCAGTCTGTG CAGACAGACA GACAGAAGCC CAAGCCATCA GGCTACCACT 
GGGGACAGGA AGGCAGAAGG AAAGAAATGC ACAGACGCAC TTTATGTCGC AGCCCCAGAG 
GGCTACAAGC CAGAGGCCAG CCCTTCTCTC CACCACGGCG AGACCGGACT CAGAGGCTCA 
GAGAGGCCAC CCATGGGCAT GGGGAAGGGC TTCTCTGAGC CCAAGGGGAA AGGGCCAGGT 
CCCCAGAAGT CACTGGCTGA AACAGGCAAG CCCAGCGGTA TGAAAAGGTC ACCCTCTGCC 
ACCGTGCAGA GCTCTCTCCG CTCAGCTGCC CCCCCAGAAA AGTCTCTGAG TTACTCAGCC 
AGCTTTCCCG AGGCCCAGCC TGGAGTGCGA GAGGTCCCTG CAGCCAACAG CAGCCCCTCA 
TCTGCCAAGG CTACAGGGGG GACCTCAGAG TTCCCAGCCC CCAGCAGCAG GGACCACAGG 
AAGCTTCAGT CTGGAGGAGA CGGCCGAAGC CAAATGATAA AGAGTGACTC TCTGCCCTCC 
TTCCGCCTCT CCACCTCTGC TCTGGAGTCA CATTTCCAGG ATCCACAGGT GCCCATCGCA 
TCAGGCCACC GAGGCAGGGC ACTGTCAGTA ACTGCTGCCA CAGGAGAACC CAAAGGGAGA 
GAGCTCGCCC AGCCTCCCCC AGTCAGGAAA CAGAATGCGT GCAGAGAGGC GACCAGAGCA 
CCCCCAGCCC CAAGCACAGA TCGCTCCCTC CCTCTTTCCT CAGAGAAAGA CTTCGTGGTT 
CGGCAGAGAA GGGGCAAGGA GACCTTAAGG AGCAGTCCTC ACAAAAAGGC CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_017315527.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171016.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X1, mRNA.

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CloneID	OMu49069
Clone ID Related Accession (Same CDS sequence)	XM_017315527.1
Accession Version	XM_017315527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AAGTTTCCGA GGCGCCTGAG CCCGTGCCCC GGGGCTGCAG CGGACACGGC 
GCCCGGACCC TAGTCTCTTC GGCGGCAGCC GTGTCCTCGG AGGGCGCTTC CTCAGCGGAG 
TCATCCTCTG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CCAGCCGCTT CTCCTGCAGG 
TCGCAGCCGC CGCGGCCGCC GGGCGGCGCC CTGGGAACCC GGCTACCCGC CGCGTGGGCT 
CCCGCGCGCG TGGCTCTGGA GCGTGGAGTC CCTACCCTGC CGCTGCCGCA CCCGGGAGGA 
GCGGTGCTGC CGGTGCCCCA GGTCAGCAGC GCATCCCAAG AGGAGCAGGA TGAAGAGCTT 
GACCACATAC TGTCTCCGCC ACCCATGCCG TTTCGGAAAT GCAGCAACCC AGATGTGGCC 
TGCGGCCTCG GAAAATCACT GAAGTACAAG AGACAGCTTA GTGAGGATGG GAAGCAGCTG 
CGGCGGGGGA GCCTGGGAGG AGCCCTCACA GGGAGGTACC TCCTTCCAAA CCCGGTAGCA 
GGACAGGCCT GGCCTGCTTC GGCGGAGACG TCCAACCTCG TGCGCATGCG CAGCCAGGCC 
CTTGGCCAAT CGGCTCCCTC GCTCACAGCC AGCTTGGAAG GGCAACCTCC GAAGAGCCAT 
TTCAACTCAG CTCGACATCG GCAGAGACTA GTGGACCCAG CTGCTTCAAA AAAGGAGCTG 
AGCCTCCCCC GAAGAGGAAG TCTGGTAGAT TCCCAGAAGT GGAATTGCTT GGTCAAACGT 
TGCCGAACAA GCAACCGGAA GAGTTTGATA GGCAATGGCC AGTCTCCAGC ACTGCCTCGA 
CCACACTCAC CTCTCTCTGC TCATGCAGGA AATAGCCCTC AAGACAGTCC AAGGAATTTC 
TCCCCCAGTG CCTCAGCCCA TTTCTCATTT GCGAGGAGAA ATGACAGAAC GGATGGGCGC 
CGCTGGTCTC TGGCTTCTCT CCCTTCCTCA GGCTATGGGA CAAATACCCC AAGCTCCACC 
GTCTCCTCAT CCTGTTCTTC CCAGGAGAAG TTGCACCAGC TACCATACCA GCCAACCCCA 
GATGAATTAC ACTTCTTATC CAAACACTTC TGCACAACAG AAAGTATCGC CACTGAGAAC 
CGGTGCAGAA ACACACCCAT GCGTCCACGT TCCCGGAGTC TCAGCCCTGG ACGGTCCCCC 
GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG 
GCCACAGCTC AGATGGAAGA GCGTCTGAAG GAGATCATCA CCAGCTACTC TCCAGACCAT 
GTTCTCCCCT TGGCAGATGG GGTACTTAGT TTCACTCACC ATCAGATCAT TGAGCTGGCT 
CGGGACTGTT TGGATAAATC TCACCAGGGT CTCATCACGT CGAGATACTT TTTTGAGTTG 
CAGCACAAAC TGGACAAGTT GCTCCAGGAG GCTCACGATC GCTCTGAAAG TGGAGAACTG 
GCATTTATCA AGCAACTAGT CCGAAAGATC CTAATTGTCA TTGCCCGCCC CGCTCGGTTA 
TTGGAGTGTT TGGAATTTGA TCCTGAAGAA TTTTATTACC TATTGGAAGC TGCAGAAGGC 
CATGCCAAAG AAGGCCAAGG AATCAAAACT GACATCCCTA GGTATATCAT CAGCCAGCTG 
GGACTCAATA AGGACCCTTT AGAAGAAATG GCTCAGTTGG GGAATTACGA CAGTAGGACA 
GCAGAGACAC CAGAGATGGA TGAGTCAGTG AGTAGCTCAA ATACTTCCCT GAGACTTCGA 
AGGAAACCCC GAGAGAGTGA TTTTGAAACA ATTAAATTGA TCAGCAATGG AGCCTACGGG 
GCAGTCTACT TTGTTCGGCA CAAAGAGTCC CGCCAGAGGT TTGCCATGAA GAAGATCAAC 
AAGCAGAACC TCATCCTTCG GAACCAGATC CAGCAGGCCT TCGTGGAGCG AGACATCCTG 
ACTTTCGCAG AGAACCCCTT TGTGGTCAGC ATGTATTGCT CCTTTGAAAC GAGGCGTCAC 
TTATGCATGG TCATGGAGTA TGTAGAAGGG GGAGACTGTG CGACCCTAAT GAAAAACATG 
GGACCTCTCC CAGTTGATAT GGCCAGAATG TATTTCGCCG AGACCGTCTT GGCCTTGGAG 
TACCTGCATA ATTACGGAAT CGTACACAGG GACTTGAAGC CAGACAACCT GTTGGTCACC 
TCCATGGGGC ACATAAAACT GACTGACTTC GGCTTGTCTA AGGTGGGATT AATGAGCATG 
ACCACCAATC TCTATGAAGG CCACATAGAG AAGGACGCTC GAGAGTTCTT AGATAAACAG 
GTCTGTGGTA CACCTGAGTA CATTGCCCCC GAGGTGATTC TGAGACAGGG CTACGGGAAA 
CCCGTGGACT GGTGGGCCAT GGGCATTATC CTCTATGAAT TTCTGGTCGG ATGTGTGCCT 
TTCTTTGGGG ACACTCCAGA AGAGCTATTT GGACAAGTCA TCAGTGATGA AATCAACTGG 
CCTGAAAAGG ACGAGGCCCC TCCTCCAGAC GCTCAGGAGC TGATTACCTT GCTCCTCAGG 
CAGAATCCGC TGGAGAGGCT GGGAACAGGT GGAGCCTATG AAGTGAAGCA GCATCGTTTC 
TTCCGCTCCT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CGGAATTTAT TCCCCAACTG 
GAATCGGAGG ATGACACAAG TTATTTTGAT ACTCGGTCAG AGAAGTATCA TCACATGGAG 
ACGGAGGAGG AGGACGATAC AAACGATGAG GACTTCACCG TGGAGATAAG GCAGTTTTCT 
TCCTGTTCAC ACAGGTTTTC AAAAGTTTTC AGCAGTATAG ATCGCATAAC TCAAAATTCA 
GGAGAAGACA AAGATGACTC TGAGGACAAG ACCAAAAGCA CAACGTTGCC ATCCACAGAG 
ACACTCAGCT GGAGTTCCGA ATACTCTGAA ATGCAACAGT TATCGACCTC CAACTCTTCA 
GATACTGAAA GCAACAGGTG CAAACTCAGC TCTGGCTTGC TCCCCAAGCT GGCTATTTCG 
ACAGATGGGG AACAAGATGA GGCTGTCCCT TGCTCTGGAG ACCCCAGAGA GGAGCCAGAG 
AAACCTGTCC CTCCCTCTGA GGAGTGTACT CAGGAGGAGC CCGAGGTCAC CACCCCAGCC 
AGCACCATCA GCAGTTCCAC ACTGTCAGTT GGCAGTTTTT CAGAGCACTT GGATCAGATA 
AATGGGCGAA GCGAGTGTGT GGACAGTACA GATAATTCCT CAAAGCCATC CAGTGAACCC 
ACTTCTCACG TGGCTCGACA GCGCTTAGAA AGCACAGAGA AAAAGAAAAT TTCTGGGAAA 
GTCACAAAGT CCCTCTCGGC CAGTGCTCTG TCCCTCATGA TCCCAGGAGA TATGTTCGCT 
GTATCTCCAT TGGGAAGCCC AATGTCCCCA CACTCCCTGT CTTCAGACCC TTCTTCTTCA 
CGGGATTCCT CTCCCAGCCG AGACTCTTCT GCAGCATCTG CCAGTCCGCA TCAGCCCATT 
GTCATCCACA GCTCAGGCAA GAACTATGGG TTCACCATCC GTGCTATCCG CGTGTACGTG 
GGGGACAGTG ACATCTACAC AGTGCACCAT ATCGTCTGGA ACGTAGAAGA AGGAAGTCCC 
GCATACCAGG CAGGACTGAA GGCCGGGGAT CTGATCACAC ACATCAACGG AGAGCCGGTG 
CACGGCCTCG TCCACACGGA AGTTATCGAG CTCCTGCTGA AGAGTGGGAA TAAGGTGTCT 
ATCACCACTA CTCCATTTGA AAACACATCA ATCAAAACGG GACCAGCCAG GAGAAACAGT 
TACAAGGGCC GGATGGTGAG ACGAAGCAAG AAGTCCAAGA AGAAGGAGAG TCTAGAAAGG 
AGGAGATCTC TCTTCAAGAA GCTGGCCAAG CAGCCTTCTC CTTTGCTCCA CACCAGCCGA 
AGTTTCTCCT GCTTAAACCG GTCCCTGTCA TCTGGAGAGA GCCTCCCGGG TTCCCCAACT 
CACAGCTTGT CCCCGAGGTC TCCAACACCC AGTTATCGTT CTACTCCCGA TTTTCCGTCA 
GGTACAAATT CCTCCCAGAG CAGCTCCCCA AGTTCAAGTG CCCCCAATTC TCCAGCAGGT 
TCAGGGCACA TCCGGCCCAG CACCCTCCAT GGCCTGGCTC CCAAACTCAG CGGGCAGCGA 
TACCGCTCTG GAAGACGGAA GTCGGCTGGC AGCATCCCTC TCTCCCCGCT GGCCAGGACA 
CCCTCTCCCA CTCCACAGCC TACCTCTCCT CAGCGTTCAC CATCCCCACT GTTGGGACAC 
TCACTGGGCA ATGCCAAGAT CACTCAGGCC TTTCCTAGCA AGATGCACTC TCCCCCCACA 
ATCGTCAGAC ACATCGTGAG GCCCAAGAGT GCAGAGCCGC CCCGCTCCCC ACTGCTGAAA 
CGGGTGCAGT CGGAGGAAAA GTTGTCACCC TCCTATGGCA GTGACAAGAA GCTTCTGTGC 
TCCCGCAAGC ATAGCCTAGA GGTGACACAA GAGGAGGTAC AGAGGGAGCA GTGTCAGCGG 
GAAGTGACAC TGCAGAGCCT GGAAGAGAAT GTGTGTGACG CTCCTTCCCT CAGTCGGGCC 
AGGCCAGTGG AGCAAGGCTG TCTGAAACGC CCCGTGTCCC GGAAGGTGGG CAGGCAAGAG 
TCTGTGGATG ACCTGGACCG GGACAAGCTG AAAGCCAAGG TTGTCGTAAA GAAACCAGAA 
GAGAAACATG AATCGCACCA GAAACCTCAC AGCCTTGGTG GTGATTCGGA AAGCTATGCT 
CTCTTCAGGC TAGAGGAGAG AGAGAAAAAA GTGTACTCCA AGGGGTTGGA AAGGTCAGGC 
CATTTTGAAA ACACATCAGC AGAGTTGCCT TCTGTGGGCA GCCTGCTGAA GGACACTCTT 
CACAAGCAGG CCAGCGTGAG GGCCAGCGAG GGGGTGACCT CAGACGGGGC AGCTTGCAGC 
CTGACACCAG GGGAGCACAG CCAGTCTCTA GGTGACTTTA AGCGGGCCTC AGCTTCTGGC 
ATTCTCCATG ATAGTGTGTG CCCCATCTCT GATAGGCCTG CTCCTGGAAA GGTTGAATAC 
TCGGAGAAGG CCTCTCAGGC CAAAGAGCTC CTTCGAAGTG AAAAACTAGA CAGCAAGCTG 
GCCAATATTG ATTACCTCAG AAAGAAAATG TCATTGGATG ACAAAGATGA CAGCCACTGT 
GCCATCCTGA AACCCAAGAT AACATCTAGC GCCCATGAAT GTCTGCCAGG GAACCCCATA 
CGGCCCATGG CAGGGCAACA AGAGACCCCG CCAGCCTCTG AAAACCGGGC ATTCATCAAC 
AGTACCCACA CACCTCAGAT GAGTGCAGTT TCCTTTGTTC CTCTCAAAGC CTTAGCTGGC 
CGGGTAGAGA ACGGAGGGGA GAAAGCAGGC TTAGCTGCTC CCGAGTCCCC TGTCAGGAAG 
AGCCCCTCCG AGTATAAGCT AGAGGGCAGG TCAGTTTCAT GTCTCAAGCC GATCGAGGGC 
ACACTGGACA TTGCTCTCCT GTCTGGACCT CACGCCTCCA AAACAGAGTT GCTTTCCCCA 
GAGCCTGCAC AGAGTCCCAG CCCAGGCATC AACGTGGGAC CATGTGTGCC ACTAGCTCTT 
CCTGGGAGCA GTGGGAAAAA GGGAGACTCC ACCAGCCTGA GAGAGCCTTC CTCAGCCAAC 
TTAAAAGTAA ATAAATCTTA TCTGCTGGAG CCTCGGTTCC TACCCCCGAG CCGGGCTCTC 
CAGGACTCTC TCGCAGCCTC TGGGCCAGAA CCGAAGTCAA AGCCGGAAAG GAAGCTCATT 
CATCCTTCTG CCAGGAGCCC AGCAACTGTC ACAGAGAGCA ATCTTCAGCA GAAAGAGGGT 
GGTCCCGCCA CACACCAAGA CCGCTCCACT GACACCAGGA ACCTCCCTGG CCCAGGGCAG 
ACCCTACACA ATGTGGACCT ACCCAGGCTG TGTACACGTG CCCCACTCCC ACCGGAAGGG 
ACGCCCGCAA AGGAGAAGCC ATGTCTGAAG GAACCCTCTG CCAAGGTGAA AAGCGAGTGG 
TCTGCCGTGA GGGATGACGG ACACAGAGAT CCCTGTGCGA AGCTGTGCCC GGCAGAGACT 
GGTAAAGCCA GCGACAGTTC CAAACCCCTG CCTTCCGGGG GGAGGACCCA ACCCGATTTC 
TACAAGCAGA CCCAGACTTC GGAGAAAGCA TGGGCGCATG CAAAAACAAA CCACAAAGAT 
AGCCAAGATG AGGTGAAGTC CCTGGCCAGG GAGGACTCAG CTTCACTTTT ATATGAAAAG 
GAGATAGGCC GGGCACGAAA AGGTCCTGAA CCCAAACCGG AAGTTCCTGC TACCCGGTGC 
CCTCCTCAGC CACCAGGAAT TGAGGGTGAG AAGCGAGAAA AGCTCTCCGC TGCCCCCTCT 
TTGCAGAAAC AGGCTCCCAA AGAGCCAGAC AGGAAGGAAC AGACTTCGCA AAGGCCTGGA 
GGTAGTGGCC CTCAACAACC CCCACCCACC AAAGAGCTGT CTAACTCAGC ATCCTGGCAG 
CACGGCAGTT CTCCGAGTCA CACTTTAAAG AAGGAGCCCG GGACCAAAGC TGCCGCTGCA 
GAACCAAGCA CCAGCCTTCA TGACACTCCC CGATCTGCTA CAGCCACCAC CACTGCCATT 
GCCACCACCA CCACTACCAC CAGTGCCGGG CACAGTGACT GCAGTAGCCA TAAGGCCCGG 
CCTGGCCCTG ACCCCAGCCC TTCAAAGTCT AAGCACCAAG ACAGGTCCCT CTCCTCACAG 
AAGCTGAGTG CTGGCTCTGC AAAAGGCAAA GAGCCTGTCA CTCAACCCCT GGGTGGTTCC 
ATCAGAGAAG GCAAGGGTGG CAGCAAGGGT CCAGTGGACA CATTTTCTGC TGTCCTGACC 
ACCCAGGGCA AAGCAAGTGA TGTGCTTGTG CAGGGAGAAG GTCGGGTCTC AATCATTGTC 
CACACTGAAG AGTGTCCTCT CGATGCCAAA CTGAAAAACA CCAATGGAGG GTGTCCCCCA 
GAGATGCAGG CGAAGCATCC ACCCAGACAA GGACATCTCA GTGAAGCAGC AGACCAGAAG 
CCACTCATTG CTGGTGAGAA GCAAAGCCCG TCTCCAAAGC ATCCCAAACC ATCCACTGTG 
AAAGATTACC CCAGTCTGTG CAGACAGACA GACAGAAGCC CAAGCCATCA GGCTACCACT 
GGGGACAGGA AGGCAGAAGG AAAGAAATGC ACAGACGCAC TTTATGTCGC AGCCCCAGAG 
GGCTACAAGC CAGAGGCCAG CCCTTCTCTC CACCACGGCG AGACCGGACT CAGAGGCTCA 
GAGAGGCCAC CCATGGGCAT GGGGAAGGGC TTCTCTGAGC CCAAGGGGAA AGGGCCAGGT 
CCCCAGAAGT CACTGGCTGA AACAGGCAAG CCCAGCGGTA TGAAAAGGTC ACCCTCTGCC 
ACCGTGCAGA GCTCTCTCCG CTCAGCTGCC CCCCCAGAAA AGTCTCTGAG TTACTCAGCC 
AGCTTTCCCG AGGCCCAGCC TGGAGTGCGA GAGGTCCCTG CAGCCAACAG CAGCCCCTCA 
TCTGCCAAGG CTACAGGGGG GACCTCAGAG TTCCCAGCCC CCAGCAGCAG GGACCACAGG 
AAGCTTCAGT CTGGAGGAGA CGGCCGAAGC CAAATGATAA AGAGTGACTC TCTGCCCTCC 
TTCCGCCTCT CCACCTCTGC TCTGGAGTCA CATTTCCAGG ATCCACAGGT GCCCATCGCA 
TCAGGCCACC GAGGCAGGGC ACTGTCAGTA ACTGCTGCCA CAGGAGAACC CAAAGGGAGA 
GAGCTCGCCC AGCCTCCCCC AGTCAGGAAA CAGAATGCGT GCAGAGAGGC GACCAGAGCA 
CCCCCAGCCC CAAGCACAGA TCGCTCCCTC CCTCTTTCCT CAGAGAAAGA CTTCGTGGTT 
CGGCAGAGAA GGGGCAAGGA GACCTTAAGG AGCAGTCCTC ACAAAAAGGC CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171016.1
CDS	908..8884
Translation	MGEKVSEAPEPVPRGCSGHGARTLVSSAAAVSSEGASSAESSSGSETLSEEGEPSRFSCRSQPPRPPGGALGTRLPAAWAPARVALERGVPTLPLPHPGGAVLPVPQVSSASQEEQDEELDHILSPPPMPFRKCSNPDVACGLGKSLKYKRQLSEDGKQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLEGQPPKSHFNSARHRQRLVDPAASKKELSLPRRGSLVDSQKWNCLVKRCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_017315527.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315527.1

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCCGA GGCGCCTGAG CCCGTGCCCC GGGGCTGCAG CGGACACGGC 
GCCCGGACCC TAGTCTCTTC GGCGGCAGCC GTGTCCTCGG AGGGCGCTTC CTCAGCGGAG 
TCATCCTCTG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CCAGCCGCTT CTCCTGCAGG 
TCGCAGCCGC CGCGGCCGCC GGGCGGCGCC CTGGGAACCC GGCTACCCGC CGCGTGGGCT 
CCCGCGCGCG TGGCTCTGGA GCGTGGAGTC CCTACCCTGC CGCTGCCGCA CCCGGGAGGA 
GCGGTGCTGC CGGTGCCCCA GGTCAGCAGC GCATCCCAAG AGGAGCAGGA TGAAGAGCTT 
GACCACATAC TGTCTCCGCC ACCCATGCCG TTTCGGAAAT GCAGCAACCC AGATGTGGCC 
TGCGGCCTCG GAAAATCACT GAAGTACAAG AGACAGCTTA GTGAGGATGG GAAGCAGCTG 
CGGCGGGGGA GCCTGGGAGG AGCCCTCACA GGGAGGTACC TCCTTCCAAA CCCGGTAGCA 
GGACAGGCCT GGCCTGCTTC GGCGGAGACG TCCAACCTCG TGCGCATGCG CAGCCAGGCC 
CTTGGCCAAT CGGCTCCCTC GCTCACAGCC AGCTTGGAAG GGCAACCTCC GAAGAGCCAT 
TTCAACTCAG CTCGACATCG GCAGAGACTA GTGGACCCAG CTGCTTCAAA AAAGGAGCTG 
AGCCTCCCCC GAAGAGGAAG TCTGGTAGAT TCCCAGAAGT GGAATTGCTT GGTCAAACGT 
TGCCGAACAA GCAACCGGAA GAGTTTGATA GGCAATGGCC AGTCTCCAGC ACTGCCTCGA 
CCACACTCAC CTCTCTCTGC TCATGCAGGA AATAGCCCTC AAGACAGTCC AAGGAATTTC 
TCCCCCAGTG CCTCAGCCCA TTTCTCATTT GCGAGGAGAA ATGACAGAAC GGATGGGCGC 
CGCTGGTCTC TGGCTTCTCT CCCTTCCTCA GGCTATGGGA CAAATACCCC AAGCTCCACC 
GTCTCCTCAT CCTGTTCTTC CCAGGAGAAG TTGCACCAGC TACCATACCA GCCAACCCCA 
GATGAATTAC ACTTCTTATC CAAACACTTC TGCACAACAG AAAGTATCGC CACTGAGAAC 
CGGTGCAGAA ACACACCCAT GCGTCCACGT TCCCGGAGTC TCAGCCCTGG ACGGTCCCCC 
GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG 
GCCACAGCTC AGATGGAAGA GCGTCTGAAG GAGATCATCA CCAGCTACTC TCCAGACCAT 
GTTCTCCCCT TGGCAGATGG GGTACTTAGT TTCACTCACC ATCAGATCAT TGAGCTGGCT 
CGGGACTGTT TGGATAAATC TCACCAGGGT CTCATCACGT CGAGATACTT TTTTGAGTTG 
CAGCACAAAC TGGACAAGTT GCTCCAGGAG GCTCACGATC GCTCTGAAAG TGGAGAACTG 
GCATTTATCA AGCAACTAGT CCGAAAGATC CTAATTGTCA TTGCCCGCCC CGCTCGGTTA 
TTGGAGTGTT TGGAATTTGA TCCTGAAGAA TTTTATTACC TATTGGAAGC TGCAGAAGGC 
CATGCCAAAG AAGGCCAAGG AATCAAAACT GACATCCCTA GGTATATCAT CAGCCAGCTG 
GGACTCAATA AGGACCCTTT AGAAGAAATG GCTCAGTTGG GGAATTACGA CAGTAGGACA 
GCAGAGACAC CAGAGATGGA TGAGTCAGTG AGTAGCTCAA ATACTTCCCT GAGACTTCGA 
AGGAAACCCC GAGAGAGTGA TTTTGAAACA ATTAAATTGA TCAGCAATGG AGCCTACGGG 
GCAGTCTACT TTGTTCGGCA CAAAGAGTCC CGCCAGAGGT TTGCCATGAA GAAGATCAAC 
AAGCAGAACC TCATCCTTCG GAACCAGATC CAGCAGGCCT TCGTGGAGCG AGACATCCTG 
ACTTTCGCAG AGAACCCCTT TGTGGTCAGC ATGTATTGCT CCTTTGAAAC GAGGCGTCAC 
TTATGCATGG TCATGGAGTA TGTAGAAGGG GGAGACTGTG CGACCCTAAT GAAAAACATG 
GGACCTCTCC CAGTTGATAT GGCCAGAATG TATTTCGCCG AGACCGTCTT GGCCTTGGAG 
TACCTGCATA ATTACGGAAT CGTACACAGG GACTTGAAGC CAGACAACCT GTTGGTCACC 
TCCATGGGGC ACATAAAACT GACTGACTTC GGCTTGTCTA AGGTGGGATT AATGAGCATG 
ACCACCAATC TCTATGAAGG CCACATAGAG AAGGACGCTC GAGAGTTCTT AGATAAACAG 
GTCTGTGGTA CACCTGAGTA CATTGCCCCC GAGGTGATTC TGAGACAGGG CTACGGGAAA 
CCCGTGGACT GGTGGGCCAT GGGCATTATC CTCTATGAAT TTCTGGTCGG ATGTGTGCCT 
TTCTTTGGGG ACACTCCAGA AGAGCTATTT GGACAAGTCA TCAGTGATGA AATCAACTGG 
CCTGAAAAGG ACGAGGCCCC TCCTCCAGAC GCTCAGGAGC TGATTACCTT GCTCCTCAGG 
CAGAATCCGC TGGAGAGGCT GGGAACAGGT GGAGCCTATG AAGTGAAGCA GCATCGTTTC 
TTCCGCTCCT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CGGAATTTAT TCCCCAACTG 
GAATCGGAGG ATGACACAAG TTATTTTGAT ACTCGGTCAG AGAAGTATCA TCACATGGAG 
ACGGAGGAGG AGGACGATAC AAACGATGAG GACTTCACCG TGGAGATAAG GCAGTTTTCT 
TCCTGTTCAC ACAGGTTTTC AAAAGTTTTC AGCAGTATAG ATCGCATAAC TCAAAATTCA 
GGAGAAGACA AAGATGACTC TGAGGACAAG ACCAAAAGCA CAACGTTGCC ATCCACAGAG 
ACACTCAGCT GGAGTTCCGA ATACTCTGAA ATGCAACAGT TATCGACCTC CAACTCTTCA 
GATACTGAAA GCAACAGGTG CAAACTCAGC TCTGGCTTGC TCCCCAAGCT GGCTATTTCG 
ACAGATGGGG AACAAGATGA GGCTGTCCCT TGCTCTGGAG ACCCCAGAGA GGAGCCAGAG 
AAACCTGTCC CTCCCTCTGA GGAGTGTACT CAGGAGGAGC CCGAGGTCAC CACCCCAGCC 
AGCACCATCA GCAGTTCCAC ACTGTCAGTT GGCAGTTTTT CAGAGCACTT GGATCAGATA 
AATGGGCGAA GCGAGTGTGT GGACAGTACA GATAATTCCT CAAAGCCATC CAGTGAACCC 
ACTTCTCACG TGGCTCGACA GCGCTTAGAA AGCACAGAGA AAAAGAAAAT TTCTGGGAAA 
GTCACAAAGT CCCTCTCGGC CAGTGCTCTG TCCCTCATGA TCCCAGGAGA TATGTTCGCT 
GTATCTCCAT TGGGAAGCCC AATGTCCCCA CACTCCCTGT CTTCAGACCC TTCTTCTTCA 
CGGGATTCCT CTCCCAGCCG AGACTCTTCT GCAGCATCTG CCAGTCCGCA TCAGCCCATT 
GTCATCCACA GCTCAGGCAA GAACTATGGG TTCACCATCC GTGCTATCCG CGTGTACGTG 
GGGGACAGTG ACATCTACAC AGTGCACCAT ATCGTCTGGA ACGTAGAAGA AGGAAGTCCC 
GCATACCAGG CAGGACTGAA GGCCGGGGAT CTGATCACAC ACATCAACGG AGAGCCGGTG 
CACGGCCTCG TCCACACGGA AGTTATCGAG CTCCTGCTGA AGAGTGGGAA TAAGGTGTCT 
ATCACCACTA CTCCATTTGA AAACACATCA ATCAAAACGG GACCAGCCAG GAGAAACAGT 
TACAAGGGCC GGATGGTGAG ACGAAGCAAG AAGTCCAAGA AGAAGGAGAG TCTAGAAAGG 
AGGAGATCTC TCTTCAAGAA GCTGGCCAAG CAGCCTTCTC CTTTGCTCCA CACCAGCCGA 
AGTTTCTCCT GCTTAAACCG GTCCCTGTCA TCTGGAGAGA GCCTCCCGGG TTCCCCAACT 
CACAGCTTGT CCCCGAGGTC TCCAACACCC AGTTATCGTT CTACTCCCGA TTTTCCGTCA 
GGTACAAATT CCTCCCAGAG CAGCTCCCCA AGTTCAAGTG CCCCCAATTC TCCAGCAGGT 
TCAGGGCACA TCCGGCCCAG CACCCTCCAT GGCCTGGCTC CCAAACTCAG CGGGCAGCGA 
TACCGCTCTG GAAGACGGAA GTCGGCTGGC AGCATCCCTC TCTCCCCGCT GGCCAGGACA 
CCCTCTCCCA CTCCACAGCC TACCTCTCCT CAGCGTTCAC CATCCCCACT GTTGGGACAC 
TCACTGGGCA ATGCCAAGAT CACTCAGGCC TTTCCTAGCA AGATGCACTC TCCCCCCACA 
ATCGTCAGAC ACATCGTGAG GCCCAAGAGT GCAGAGCCGC CCCGCTCCCC ACTGCTGAAA 
CGGGTGCAGT CGGAGGAAAA GTTGTCACCC TCCTATGGCA GTGACAAGAA GCTTCTGTGC 
TCCCGCAAGC ATAGCCTAGA GGTGACACAA GAGGAGGTAC AGAGGGAGCA GTGTCAGCGG 
GAAGTGACAC TGCAGAGCCT GGAAGAGAAT GTGTGTGACG CTCCTTCCCT CAGTCGGGCC 
AGGCCAGTGG AGCAAGGCTG TCTGAAACGC CCCGTGTCCC GGAAGGTGGG CAGGCAAGAG 
TCTGTGGATG ACCTGGACCG GGACAAGCTG AAAGCCAAGG TTGTCGTAAA GAAACCAGAA 
GAGAAACATG AATCGCACCA GAAACCTCAC AGCCTTGGTG GTGATTCGGA AAGCTATGCT 
CTCTTCAGGC TAGAGGAGAG AGAGAAAAAA GTGTACTCCA AGGGGTTGGA AAGGTCAGGC 
CATTTTGAAA ACACATCAGC AGAGTTGCCT TCTGTGGGCA GCCTGCTGAA GGACACTCTT 
CACAAGCAGG CCAGCGTGAG GGCCAGCGAG GGGGTGACCT CAGACGGGGC AGCTTGCAGC 
CTGACACCAG GGGAGCACAG CCAGTCTCTA GGTGACTTTA AGCGGGCCTC AGCTTCTGGC 
ATTCTCCATG ATAGTGTGTG CCCCATCTCT GATAGGCCTG CTCCTGGAAA GGTTGAATAC 
TCGGAGAAGG CCTCTCAGGC CAAAGAGCTC CTTCGAAGTG AAAAACTAGA CAGCAAGCTG 
GCCAATATTG ATTACCTCAG AAAGAAAATG TCATTGGATG ACAAAGATGA CAGCCACTGT 
GCCATCCTGA AACCCAAGAT AACATCTAGC GCCCATGAAT GTCTGCCAGG GAACCCCATA 
CGGCCCATGG CAGGGCAACA AGAGACCCCG CCAGCCTCTG AAAACCGGGC ATTCATCAAC 
AGTACCCACA CACCTCAGAT GAGTGCAGTT TCCTTTGTTC CTCTCAAAGC CTTAGCTGGC 
CGGGTAGAGA ACGGAGGGGA GAAAGCAGGC TTAGCTGCTC CCGAGTCCCC TGTCAGGAAG 
AGCCCCTCCG AGTATAAGCT AGAGGGCAGG TCAGTTTCAT GTCTCAAGCC GATCGAGGGC 
ACACTGGACA TTGCTCTCCT GTCTGGACCT CACGCCTCCA AAACAGAGTT GCTTTCCCCA 
GAGCCTGCAC AGAGTCCCAG CCCAGGCATC AACGTGGGAC CATGTGTGCC ACTAGCTCTT 
CCTGGGAGCA GTGGGAAAAA GGGAGACTCC ACCAGCCTGA GAGAGCCTTC CTCAGCCAAC 
TTAAAAGTAA ATAAATCTTA TCTGCTGGAG CCTCGGTTCC TACCCCCGAG CCGGGCTCTC 
CAGGACTCTC TCGCAGCCTC TGGGCCAGAA CCGAAGTCAA AGCCGGAAAG GAAGCTCATT 
CATCCTTCTG CCAGGAGCCC AGCAACTGTC ACAGAGAGCA ATCTTCAGCA GAAAGAGGGT 
GGTCCCGCCA CACACCAAGA CCGCTCCACT GACACCAGGA ACCTCCCTGG CCCAGGGCAG 
ACCCTACACA ATGTGGACCT ACCCAGGCTG TGTACACGTG CCCCACTCCC ACCGGAAGGG 
ACGCCCGCAA AGGAGAAGCC ATGTCTGAAG GAACCCTCTG CCAAGGTGAA AAGCGAGTGG 
TCTGCCGTGA GGGATGACGG ACACAGAGAT CCCTGTGCGA AGCTGTGCCC GGCAGAGACT 
GGTAAAGCCA GCGACAGTTC CAAACCCCTG CCTTCCGGGG GGAGGACCCA ACCCGATTTC 
TACAAGCAGA CCCAGACTTC GGAGAAAGCA TGGGCGCATG CAAAAACAAA CCACAAAGAT 
AGCCAAGATG AGGTGAAGTC CCTGGCCAGG GAGGACTCAG CTTCACTTTT ATATGAAAAG 
GAGATAGGCC GGGCACGAAA AGGTCCTGAA CCCAAACCGG AAGTTCCTGC TACCCGGTGC 
CCTCCTCAGC CACCAGGAAT TGAGGGTGAG AAGCGAGAAA AGCTCTCCGC TGCCCCCTCT 
TTGCAGAAAC AGGCTCCCAA AGAGCCAGAC AGGAAGGAAC AGACTTCGCA AAGGCCTGGA 
GGTAGTGGCC CTCAACAACC CCCACCCACC AAAGAGCTGT CTAACTCAGC ATCCTGGCAG 
CACGGCAGTT CTCCGAGTCA CACTTTAAAG AAGGAGCCCG GGACCAAAGC TGCCGCTGCA 
GAACCAAGCA CCAGCCTTCA TGACACTCCC CGATCTGCTA CAGCCACCAC CACTGCCATT 
GCCACCACCA CCACTACCAC CAGTGCCGGG CACAGTGACT GCAGTAGCCA TAAGGCCCGG 
CCTGGCCCTG ACCCCAGCCC TTCAAAGTCT AAGCACCAAG ACAGGTCCCT CTCCTCACAG 
AAGCTGAGTG CTGGCTCTGC AAAAGGCAAA GAGCCTGTCA CTCAACCCCT GGGTGGTTCC 
ATCAGAGAAG GCAAGGGTGG CAGCAAGGGT CCAGTGGACA CATTTTCTGC TGTCCTGACC 
ACCCAGGGCA AAGCAAGTGA TGTGCTTGTG CAGGGAGAAG GTCGGGTCTC AATCATTGTC 
CACACTGAAG AGTGTCCTCT CGATGCCAAA CTGAAAAACA CCAATGGAGG GTGTCCCCCA 
GAGATGCAGG CGAAGCATCC ACCCAGACAA GGACATCTCA GTGAAGCAGC AGACCAGAAG 
CCACTCATTG CTGGTGAGAA GCAAAGCCCG TCTCCAAAGC ATCCCAAACC ATCCACTGTG 
AAAGATTACC CCAGTCTGTG CAGACAGACA GACAGAAGCC CAAGCCATCA GGCTACCACT 
GGGGACAGGA AGGCAGAAGG AAAGAAATGC ACAGACGCAC TTTATGTCGC AGCCCCAGAG 
GGCTACAAGC CAGAGGCCAG CCCTTCTCTC CACCACGGCG AGACCGGACT CAGAGGCTCA 
GAGAGGCCAC CCATGGGCAT GGGGAAGGGC TTCTCTGAGC CCAAGGGGAA AGGGCCAGGT 
CCCCAGAAGT CACTGGCTGA AACAGGCAAG CCCAGCGGTA TGAAAAGGTC ACCCTCTGCC 
ACCGTGCAGA GCTCTCTCCG CTCAGCTGCC CCCCCAGAAA AGTCTCTGAG TTACTCAGCC 
AGCTTTCCCG AGGCCCAGCC TGGAGTGCGA GAGGTCCCTG CAGCCAACAG CAGCCCCTCA 
TCTGCCAAGG CTACAGGGGG GACCTCAGAG TTCCCAGCCC CCAGCAGCAG GGACCACAGG 
AAGCTTCAGT CTGGAGGAGA CGGCCGAAGC CAAATGATAA AGAGTGACTC TCTGCCCTCC 
TTCCGCCTCT CCACCTCTGC TCTGGAGTCA CATTTCCAGG ATCCACAGGT GCCCATCGCA 
TCAGGCCACC GAGGCAGGGC ACTGTCAGTA ACTGCTGCCA CAGGAGAACC CAAAGGGAGA 
GAGCTCGCCC AGCCTCCCCC AGTCAGGAAA CAGAATGCGT GCAGAGAGGC GACCAGAGCA 
CCCCCAGCCC CAAGCACAGA TCGCTCCCTC CCTCTTTCCT CAGAGAAAGA CTTCGTGGTT 
CGGCAGAGAA GGGGCAAGGA GACCTTAAGG AGCAGTCCTC ACAAAAAGGC CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_017315528.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171017.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X2, mRNA.

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CloneID	OMu49070
Clone ID Related Accession (Same CDS sequence)	XM_017315528.1
Accession Version	XM_017315528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AAGTTTCCGA GGCGCCTGAG CCCGTGCCCC GGGGCTGCAG CGGACACGGC 
GCCCGGACCC TAGTCTCTTC GGCGGCAGCC GTGTCCTCGG AGGGCGCTTC CTCAGCGGAG 
TCATCCTCTG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CCAGCCGCTT CTCCTGCAGG 
TCGCAGCCGC CGCGGCCGCC GGGCGGCGCC CTGGGAACCC GGCTACCCGC CGCGTGGGCT 
CCCGCGCGCG TGGCTCTGGA GCGTGGAGTC CCTACCCTGC CGCTGCCGCA CCCGGGAGGA 
GCGGTGCTGC CGGTGCCCCA GGTCAGCAGC GCATCCCAAG AGGAGCAGGA TGAAGAGCTT 
GACCACATAC TGTCTCCGCC ACCCATGCCG TTTCGGAAAT GCAGCAACCC AGATGTGGCC 
TGCGGCCTCG GAAAATCACT GAAGTACAAG AGACAGCTTA GTGAGGATGG GAAGCAGCTG 
CGGCGGGGGA GCCTGGGAGG AGCCCTCACA GGGAGGTACC TCCTTCCAAA CCCGGTAGCA 
GGACAGGCCT GGCCTGCTTC GGCGGAGACG TCCAACCTCG TGCGCATGCG CAGCCAGGCC 
CTTGGCCAAT CGGCTCCCTC GCTCACAGCC AGCTTGGAAG GGCAACCTCC GAAGAGCCAT 
TTCAACTCAG CTCGACATCG GCAGAGACTA GTGGACCCAG CTGCTTCAAA AAAGGAGCTG 
AGCCTCCCCC GAAGAGGAAG TCTGGTAGAT TCCCAGAAGT GGAATTGCTT GGTCAAACGT 
TGCCGAACAA GCAACCGGAA GAGTTTGATA GGCAATGGCC AGTCTCCAGC ACTGCCTCGA 
CCACACTCAC CTCTCTCTGC TCATGCAGGA AATAGCCCTC AAGACAGTCC AAGGAATTTC 
TCCCCCAGTG CCTCAGCCCA TTTCTCATTT GCGAGGAGAA CGGATGGGCG CCGCTGGTCT 
CTGGCTTCTC TCCCTTCCTC AGGCTATGGG ACAAATACCC CAAGCTCCAC CGTCTCCTCA 
TCCTGTTCTT CCCAGGAGAA GTTGCACCAG CTACCATACC AGCCAACCCC AGATGAATTA 
CACTTCTTAT CCAAACACTT CTGCACAACA GAAAGTATCG CCACTGAGAA CCGGTGCAGA 
AACACACCCA TGCGTCCACG TTCCCGGAGT CTCAGCCCTG GACGGTCCCC CGCCTGCTGT 
GACCATGAAA TAATTATGAT GAACCATGTC TACAAAGAAA GGTTCCCAAA GGCCACAGCT 
CAGATGGAAG AGCGTCTGAA GGAGATCATC ACCAGCTACT CTCCAGACCA TGTTCTCCCC 
TTGGCAGATG GGGTACTTAG TTTCACTCAC CATCAGATCA TTGAGCTGGC TCGGGACTGT 
TTGGATAAAT CTCACCAGGG TCTCATCACG TCGAGATACT TTTTTGAGTT GCAGCACAAA 
CTGGACAAGT TGCTCCAGGA GGCTCACGAT CGCTCTGAAA GTGGAGAACT GGCATTTATC 
AAGCAACTAG TCCGAAAGAT CCTAATTGTC ATTGCCCGCC CCGCTCGGTT ATTGGAGTGT 
TTGGAATTTG ATCCTGAAGA ATTTTATTAC CTATTGGAAG CTGCAGAAGG CCATGCCAAA 
GAAGGCCAAG GAATCAAAAC TGACATCCCT AGGTATATCA TCAGCCAGCT GGGACTCAAT 
AAGGACCCTT TAGAAGAAAT GGCTCAGTTG GGGAATTACG ACAGTAGGAC AGCAGAGACA 
CCAGAGATGG ATGAGTCAGT GAGTAGCTCA AATACTTCCC TGAGACTTCG AAGGAAACCC 
CGAGAGAGTG ATTTTGAAAC AATTAAATTG ATCAGCAATG GAGCCTACGG GGCAGTCTAC 
TTTGTTCGGC ACAAAGAGTC CCGCCAGAGG TTTGCCATGA AGAAGATCAA CAAGCAGAAC 
CTCATCCTTC GGAACCAGAT CCAGCAGGCC TTCGTGGAGC GAGACATCCT GACTTTCGCA 
GAGAACCCCT TTGTGGTCAG CATGTATTGC TCCTTTGAAA CGAGGCGTCA CTTATGCATG 
GTCATGGAGT ATGTAGAAGG GGGAGACTGT GCGACCCTAA TGAAAAACAT GGGACCTCTC 
CCAGTTGATA TGGCCAGAAT GTATTTCGCC GAGACCGTCT TGGCCTTGGA GTACCTGCAT 
AATTACGGAA TCGTACACAG GGACTTGAAG CCAGACAACC TGTTGGTCAC CTCCATGGGG 
CACATAAAAC TGACTGACTT CGGCTTGTCT AAGGTGGGAT TAATGAGCAT GACCACCAAT 
CTCTATGAAG GCCACATAGA GAAGGACGCT CGAGAGTTCT TAGATAAACA GGTCTGTGGT 
ACACCTGAGT ACATTGCCCC CGAGGTGATT CTGAGACAGG GCTACGGGAA ACCCGTGGAC 
TGGTGGGCCA TGGGCATTAT CCTCTATGAA TTTCTGGTCG GATGTGTGCC TTTCTTTGGG 
GACACTCCAG AAGAGCTATT TGGACAAGTC ATCAGTGATG AAATCAACTG GCCTGAAAAG 
GACGAGGCCC CTCCTCCAGA CGCTCAGGAG CTGATTACCT TGCTCCTCAG GCAGAATCCG 
CTGGAGAGGC TGGGAACAGG TGGAGCCTAT GAAGTGAAGC AGCATCGTTT CTTCCGCTCC 
TTAGACTGGA ACAGTTTGCT GAGACAGAAG GCGGAATTTA TTCCCCAACT GGAATCGGAG 
GATGACACAA GTTATTTTGA TACTCGGTCA GAGAAGTATC ATCACATGGA GACGGAGGAG 
GAGGACGATA CAAACGATGA GGACTTCACC GTGGAGATAA GGCAGTTTTC TTCCTGTTCA 
CACAGGTTTT CAAAAGTTTT CAGCAGTATA GATCGCATAA CTCAAAATTC AGGAGAAGAC 
AAAGATGACT CTGAGGACAA GACCAAAAGC ACAACGTTGC CATCCACAGA GACACTCAGC 
TGGAGTTCCG AATACTCTGA AATGCAACAG TTATCGACCT CCAACTCTTC AGATACTGAA 
AGCAACAGGT GCAAACTCAG CTCTGGCTTG CTCCCCAAGC TGGCTATTTC GACAGATGGG 
GAACAAGATG AGGCTGTCCC TTGCTCTGGA GACCCCAGAG AGGAGCCAGA GAAACCTGTC 
CCTCCCTCTG AGGAGTGTAC TCAGGAGGAG CCCGAGGTCA CCACCCCAGC CAGCACCATC 
AGCAGTTCCA CACTGTCAGT TGGCAGTTTT TCAGAGCACT TGGATCAGAT AAATGGGCGA 
AGCGAGTGTG TGGACAGTAC AGATAATTCC TCAAAGCCAT CCAGTGAACC CACTTCTCAC 
GTGGCTCGAC AGCGCTTAGA AAGCACAGAG AAAAAGAAAA TTTCTGGGAA AGTCACAAAG 
TCCCTCTCGG CCAGTGCTCT GTCCCTCATG ATCCCAGGAG ATATGTTCGC TGTATCTCCA 
TTGGGAAGCC CAATGTCCCC ACACTCCCTG TCTTCAGACC CTTCTTCTTC ACGGGATTCC 
TCTCCCAGCC GAGACTCTTC TGCAGCATCT GCCAGTCCGC ATCAGCCCAT TGTCATCCAC 
AGCTCAGGCA AGAACTATGG GTTCACCATC CGTGCTATCC GCGTGTACGT GGGGGACAGT 
GACATCTACA CAGTGCACCA TATCGTCTGG AACGTAGAAG AAGGAAGTCC CGCATACCAG 
GCAGGACTGA AGGCCGGGGA TCTGATCACA CACATCAACG GAGAGCCGGT GCACGGCCTC 
GTCCACACGG AAGTTATCGA GCTCCTGCTG AAGAGTGGGA ATAAGGTGTC TATCACCACT 
ACTCCATTTG AAAACACATC AATCAAAACG GGACCAGCCA GGAGAAACAG TTACAAGGGC 
CGGATGGTGA GACGAAGCAA GAAGTCCAAG AAGAAGGAGA GTCTAGAAAG GAGGAGATCT 
CTCTTCAAGA AGCTGGCCAA GCAGCCTTCT CCTTTGCTCC ACACCAGCCG AAGTTTCTCC 
TGCTTAAACC GGTCCCTGTC ATCTGGAGAG AGCCTCCCGG GTTCCCCAAC TCACAGCTTG 
TCCCCGAGGT CTCCAACACC CAGTTATCGT TCTACTCCCG ATTTTCCGTC AGGTACAAAT 
TCCTCCCAGA GCAGCTCCCC AAGTTCAAGT GCCCCCAATT CTCCAGCAGG TTCAGGGCAC 
ATCCGGCCCA GCACCCTCCA TGGCCTGGCT CCCAAACTCA GCGGGCAGCG ATACCGCTCT 
GGAAGACGGA AGTCGGCTGG CAGCATCCCT CTCTCCCCGC TGGCCAGGAC ACCCTCTCCC 
ACTCCACAGC CTACCTCTCC TCAGCGTTCA CCATCCCCAC TGTTGGGACA CTCACTGGGC 
AATGCCAAGA TCACTCAGGC CTTTCCTAGC AAGATGCACT CTCCCCCCAC AATCGTCAGA 
CACATCGTGA GGCCCAAGAG TGCAGAGCCG CCCCGCTCCC CACTGCTGAA ACGGGTGCAG 
TCGGAGGAAA AGTTGTCACC CTCCTATGGC AGTGACAAGA AGCTTCTGTG CTCCCGCAAG 
CATAGCCTAG AGGTGACACA AGAGGAGGTA CAGAGGGAGC AGTGTCAGCG GGAAGTGACA 
CTGCAGAGCC TGGAAGAGAA TGTGTGTGAC GCTCCTTCCC TCAGTCGGGC CAGGCCAGTG 
GAGCAAGGCT GTCTGAAACG CCCCGTGTCC CGGAAGGTGG GCAGGCAAGA GTCTGTGGAT 
GACCTGGACC GGGACAAGCT GAAAGCCAAG GTTGTCGTAA AGAAACCAGA AGAGAAACAT 
GAATCGCACC AGAAACCTCA CAGCCTTGGT GGTGATTCGG AAAGCTATGC TCTCTTCAGG 
CTAGAGGAGA GAGAGAAAAA AGTGTACTCC AAGGGGTTGG AAAGGTCAGG CCATTTTGAA 
AACACATCAG CAGAGTTGCC TTCTGTGGGC AGCCTGCTGA AGGACACTCT TCACAAGCAG 
GCCAGCGTGA GGGCCAGCGA GGGGGTGACC TCAGACGGGG CAGCTTGCAG CCTGACACCA 
GGGGAGCACA GCCAGTCTCT AGGTGACTTT AAGCGGGCCT CAGCTTCTGG CATTCTCCAT 
GATAGTGTGT GCCCCATCTC TGATAGGCCT GCTCCTGGAA AGGTTGAATA CTCGGAGAAG 
GCCTCTCAGG CCAAAGAGCT CCTTCGAAGT GAAAAACTAG ACAGCAAGCT GGCCAATATT 
GATTACCTCA GAAAGAAAAT GTCATTGGAT GACAAAGATG ACAGCCACTG TGCCATCCTG 
AAACCCAAGA TAACATCTAG CGCCCATGAA TGTCTGCCAG GGAACCCCAT ACGGCCCATG 
GCAGGGCAAC AAGAGACCCC GCCAGCCTCT GAAAACCGGG CATTCATCAA CAGTACCCAC 
ACACCTCAGA TGAGTGCAGT TTCCTTTGTT CCTCTCAAAG CCTTAGCTGG CCGGGTAGAG 
AACGGAGGGG AGAAAGCAGG CTTAGCTGCT CCCGAGTCCC CTGTCAGGAA GAGCCCCTCC 
GAGTATAAGC TAGAGGGCAG GTCAGTTTCA TGTCTCAAGC CGATCGAGGG CACACTGGAC 
ATTGCTCTCC TGTCTGGACC TCACGCCTCC AAAACAGAGT TGCTTTCCCC AGAGCCTGCA 
CAGAGTCCCA GCCCAGGCAT CAACGTGGGA CCATGTGTGC CACTAGCTCT TCCTGGGAGC 
AGTGGGAAAA AGGGAGACTC CACCAGCCTG AGAGAGCCTT CCTCAGCCAA CTTAAAAGTA 
AATAAATCTT ATCTGCTGGA GCCTCGGTTC CTACCCCCGA GCCGGGCTCT CCAGGACTCT 
CTCGCAGCCT CTGGGCCAGA ACCGAAGTCA AAGCCGGAAA GGAAGCTCAT TCATCCTTCT 
GCCAGGAGCC CAGCAACTGT CACAGAGAGC AATCTTCAGC AGAAAGAGGG TGGTCCCGCC 
ACACACCAAG ACCGCTCCAC TGACACCAGG AACCTCCCTG GCCCAGGGCA GACCCTACAC 
AATGTGGACC TACCCAGGCT GTGTACACGT GCCCCACTCC CACCGGAAGG GACGCCCGCA 
AAGGAGAAGC CATGTCTGAA GGAACCCTCT GCCAAGGTGA AAAGCGAGTG GTCTGCCGTG 
AGGGATGACG GACACAGAGA TCCCTGTGCG AAGCTGTGCC CGGCAGAGAC TGGTAAAGCC 
AGCGACAGTT CCAAACCCCT GCCTTCCGGG GGGAGGACCC AACCCGATTT CTACAAGCAG 
ACCCAGACTT CGGAGAAAGC ATGGGCGCAT GCAAAAACAA ACCACAAAGA TAGCCAAGAT 
GAGGTGAAGT CCCTGGCCAG GGAGGACTCA GCTTCACTTT TATATGAAAA GGAGATAGGC 
CGGGCACGAA AAGGTCCTGA ACCCAAACCG GAAGTTCCTG CTACCCGGTG CCCTCCTCAG 
CCACCAGGAA TTGAGGGTGA GAAGCGAGAA AAGCTCTCCG CTGCCCCCTC TTTGCAGAAA 
CAGGCTCCCA AAGAGCCAGA CAGGAAGGAA CAGACTTCGC AAAGGCCTGG AGGTAGTGGC 
CCTCAACAAC CCCCACCCAC CAAAGAGCTG TCTAACTCAG CATCCTGGCA GCACGGCAGT 
TCTCCGAGTC ACACTTTAAA GAAGGAGCCC GGGACCAAAG CTGCCGCTGC AGAACCAAGC 
ACCAGCCTTC ATGACACTCC CCGATCTGCT ACAGCCACCA CCACTGCCAT TGCCACCACC 
ACCACTACCA CCAGTGCCGG GCACAGTGAC TGCAGTAGCC ATAAGGCCCG GCCTGGCCCT 
GACCCCAGCC CTTCAAAGTC TAAGCACCAA GACAGGTCCC TCTCCTCACA GAAGCTGAGT 
GCTGGCTCTG CAAAAGGCAA AGAGCCTGTC ACTCAACCCC TGGGTGGTTC CATCAGAGAA 
GGCAAGGGTG GCAGCAAGGG TCCAGTGGAC ACATTTTCTG CTGTCCTGAC CACCCAGGGC 
AAAGCAAGTG ATGTGCTTGT GCAGGGAGAA GGTCGGGTCT CAATCATTGT CCACACTGAA 
GAGTGTCCTC TCGATGCCAA ACTGAAAAAC ACCAATGGAG GGTGTCCCCC AGAGATGCAG 
GCGAAGCATC CACCCAGACA AGGACATCTC AGTGAAGCAG CAGACCAGAA GCCACTCATT 
GCTGGTGAGA AGCAAAGCCC GTCTCCAAAG CATCCCAAAC CATCCACTGT GAAAGATTAC 
CCCAGTCTGT GCAGACAGAC AGACAGAAGC CCAAGCCATC AGGCTACCAC TGGGGACAGG 
AAGGCAGAAG GAAAGAAATG CACAGACGCA CTTTATGTCG CAGCCCCAGA GGGCTACAAG 
CCAGAGGCCA GCCCTTCTCT CCACCACGGC GAGACCGGAC TCAGAGGCTC AGAGAGGCCA 
CCCATGGGCA TGGGGAAGGG CTTCTCTGAG CCCAAGGGGA AAGGGCCAGG TCCCCAGAAG 
TCACTGGCTG AAACAGGCAA GCCCAGCGGT ATGAAAAGGT CACCCTCTGC CACCGTGCAG 
AGCTCTCTCC GCTCAGCTGC CCCCCCAGAA AAGTCTCTGA GTTACTCAGC CAGCTTTCCC 
GAGGCCCAGC CTGGAGTGCG AGAGGTCCCT GCAGCCAACA GCAGCCCCTC ATCTGCCAAG 
GCTACAGGGG GGACCTCAGA GTTCCCAGCC CCCAGCAGCA GGGACCACAG GAAGCTTCAG 
TCTGGAGGAG ACGGCCGAAG CCAAATGATA AAGAGTGACT CTCTGCCCTC CTTCCGCCTC 
TCCACCTCTG CTCTGGAGTC ACATTTCCAG GATCCACAGG TGCCCATCGC ATCAGGCCAC 
CGAGGCAGGG CACTGTCAGT AACTGCTGCC ACAGGAGAAC CCAAAGGGAG AGAGCTCGCC 
CAGCCTCCCC CAGTCAGGAA ACAGAATGCG TGCAGAGAGG CGACCAGAGC ACCCCCAGCC 
CCAAGCACAG ATCGCTCCCT CCCTCTTTCC TCAGAGAAAG ACTTCGTGGT TCGGCAGAGA 
AGGGGCAAGG AGACCTTAAG GAGCAGTCCT CACAAAAAGG CCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171017.1
CDS	908..8875
Translation	MGEKVSEAPEPVPRGCSGHGARTLVSSAAAVSSEGASSAESSSGSETLSEEGEPSRFSCRSQPPRPPGGALGTRLPAAWAPARVALERGVPTLPLPHPGGAVLPVPQVSSASQEEQDEELDHILSPPPMPFRKCSNPDVACGLGKSLKYKRQLSEDGKQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLEGQPPKSHFNSARHRQRLVDPAASKKELSLPRRGSLVDSQKWNCLVKRCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_017315528.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315528.1

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCCGA GGCGCCTGAG CCCGTGCCCC GGGGCTGCAG CGGACACGGC 
GCCCGGACCC TAGTCTCTTC GGCGGCAGCC GTGTCCTCGG AGGGCGCTTC CTCAGCGGAG 
TCATCCTCTG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CCAGCCGCTT CTCCTGCAGG 
TCGCAGCCGC CGCGGCCGCC GGGCGGCGCC CTGGGAACCC GGCTACCCGC CGCGTGGGCT 
CCCGCGCGCG TGGCTCTGGA GCGTGGAGTC CCTACCCTGC CGCTGCCGCA CCCGGGAGGA 
GCGGTGCTGC CGGTGCCCCA GGTCAGCAGC GCATCCCAAG AGGAGCAGGA TGAAGAGCTT 
GACCACATAC TGTCTCCGCC ACCCATGCCG TTTCGGAAAT GCAGCAACCC AGATGTGGCC 
TGCGGCCTCG GAAAATCACT GAAGTACAAG AGACAGCTTA GTGAGGATGG GAAGCAGCTG 
CGGCGGGGGA GCCTGGGAGG AGCCCTCACA GGGAGGTACC TCCTTCCAAA CCCGGTAGCA 
GGACAGGCCT GGCCTGCTTC GGCGGAGACG TCCAACCTCG TGCGCATGCG CAGCCAGGCC 
CTTGGCCAAT CGGCTCCCTC GCTCACAGCC AGCTTGGAAG GGCAACCTCC GAAGAGCCAT 
TTCAACTCAG CTCGACATCG GCAGAGACTA GTGGACCCAG CTGCTTCAAA AAAGGAGCTG 
AGCCTCCCCC GAAGAGGAAG TCTGGTAGAT TCCCAGAAGT GGAATTGCTT GGTCAAACGT 
TGCCGAACAA GCAACCGGAA GAGTTTGATA GGCAATGGCC AGTCTCCAGC ACTGCCTCGA 
CCACACTCAC CTCTCTCTGC TCATGCAGGA AATAGCCCTC AAGACAGTCC AAGGAATTTC 
TCCCCCAGTG CCTCAGCCCA TTTCTCATTT GCGAGGAGAA CGGATGGGCG CCGCTGGTCT 
CTGGCTTCTC TCCCTTCCTC AGGCTATGGG ACAAATACCC CAAGCTCCAC CGTCTCCTCA 
TCCTGTTCTT CCCAGGAGAA GTTGCACCAG CTACCATACC AGCCAACCCC AGATGAATTA 
CACTTCTTAT CCAAACACTT CTGCACAACA GAAAGTATCG CCACTGAGAA CCGGTGCAGA 
AACACACCCA TGCGTCCACG TTCCCGGAGT CTCAGCCCTG GACGGTCCCC CGCCTGCTGT 
GACCATGAAA TAATTATGAT GAACCATGTC TACAAAGAAA GGTTCCCAAA GGCCACAGCT 
CAGATGGAAG AGCGTCTGAA GGAGATCATC ACCAGCTACT CTCCAGACCA TGTTCTCCCC 
TTGGCAGATG GGGTACTTAG TTTCACTCAC CATCAGATCA TTGAGCTGGC TCGGGACTGT 
TTGGATAAAT CTCACCAGGG TCTCATCACG TCGAGATACT TTTTTGAGTT GCAGCACAAA 
CTGGACAAGT TGCTCCAGGA GGCTCACGAT CGCTCTGAAA GTGGAGAACT GGCATTTATC 
AAGCAACTAG TCCGAAAGAT CCTAATTGTC ATTGCCCGCC CCGCTCGGTT ATTGGAGTGT 
TTGGAATTTG ATCCTGAAGA ATTTTATTAC CTATTGGAAG CTGCAGAAGG CCATGCCAAA 
GAAGGCCAAG GAATCAAAAC TGACATCCCT AGGTATATCA TCAGCCAGCT GGGACTCAAT 
AAGGACCCTT TAGAAGAAAT GGCTCAGTTG GGGAATTACG ACAGTAGGAC AGCAGAGACA 
CCAGAGATGG ATGAGTCAGT GAGTAGCTCA AATACTTCCC TGAGACTTCG AAGGAAACCC 
CGAGAGAGTG ATTTTGAAAC AATTAAATTG ATCAGCAATG GAGCCTACGG GGCAGTCTAC 
TTTGTTCGGC ACAAAGAGTC CCGCCAGAGG TTTGCCATGA AGAAGATCAA CAAGCAGAAC 
CTCATCCTTC GGAACCAGAT CCAGCAGGCC TTCGTGGAGC GAGACATCCT GACTTTCGCA 
GAGAACCCCT TTGTGGTCAG CATGTATTGC TCCTTTGAAA CGAGGCGTCA CTTATGCATG 
GTCATGGAGT ATGTAGAAGG GGGAGACTGT GCGACCCTAA TGAAAAACAT GGGACCTCTC 
CCAGTTGATA TGGCCAGAAT GTATTTCGCC GAGACCGTCT TGGCCTTGGA GTACCTGCAT 
AATTACGGAA TCGTACACAG GGACTTGAAG CCAGACAACC TGTTGGTCAC CTCCATGGGG 
CACATAAAAC TGACTGACTT CGGCTTGTCT AAGGTGGGAT TAATGAGCAT GACCACCAAT 
CTCTATGAAG GCCACATAGA GAAGGACGCT CGAGAGTTCT TAGATAAACA GGTCTGTGGT 
ACACCTGAGT ACATTGCCCC CGAGGTGATT CTGAGACAGG GCTACGGGAA ACCCGTGGAC 
TGGTGGGCCA TGGGCATTAT CCTCTATGAA TTTCTGGTCG GATGTGTGCC TTTCTTTGGG 
GACACTCCAG AAGAGCTATT TGGACAAGTC ATCAGTGATG AAATCAACTG GCCTGAAAAG 
GACGAGGCCC CTCCTCCAGA CGCTCAGGAG CTGATTACCT TGCTCCTCAG GCAGAATCCG 
CTGGAGAGGC TGGGAACAGG TGGAGCCTAT GAAGTGAAGC AGCATCGTTT CTTCCGCTCC 
TTAGACTGGA ACAGTTTGCT GAGACAGAAG GCGGAATTTA TTCCCCAACT GGAATCGGAG 
GATGACACAA GTTATTTTGA TACTCGGTCA GAGAAGTATC ATCACATGGA GACGGAGGAG 
GAGGACGATA CAAACGATGA GGACTTCACC GTGGAGATAA GGCAGTTTTC TTCCTGTTCA 
CACAGGTTTT CAAAAGTTTT CAGCAGTATA GATCGCATAA CTCAAAATTC AGGAGAAGAC 
AAAGATGACT CTGAGGACAA GACCAAAAGC ACAACGTTGC CATCCACAGA GACACTCAGC 
TGGAGTTCCG AATACTCTGA AATGCAACAG TTATCGACCT CCAACTCTTC AGATACTGAA 
AGCAACAGGT GCAAACTCAG CTCTGGCTTG CTCCCCAAGC TGGCTATTTC GACAGATGGG 
GAACAAGATG AGGCTGTCCC TTGCTCTGGA GACCCCAGAG AGGAGCCAGA GAAACCTGTC 
CCTCCCTCTG AGGAGTGTAC TCAGGAGGAG CCCGAGGTCA CCACCCCAGC CAGCACCATC 
AGCAGTTCCA CACTGTCAGT TGGCAGTTTT TCAGAGCACT TGGATCAGAT AAATGGGCGA 
AGCGAGTGTG TGGACAGTAC AGATAATTCC TCAAAGCCAT CCAGTGAACC CACTTCTCAC 
GTGGCTCGAC AGCGCTTAGA AAGCACAGAG AAAAAGAAAA TTTCTGGGAA AGTCACAAAG 
TCCCTCTCGG CCAGTGCTCT GTCCCTCATG ATCCCAGGAG ATATGTTCGC TGTATCTCCA 
TTGGGAAGCC CAATGTCCCC ACACTCCCTG TCTTCAGACC CTTCTTCTTC ACGGGATTCC 
TCTCCCAGCC GAGACTCTTC TGCAGCATCT GCCAGTCCGC ATCAGCCCAT TGTCATCCAC 
AGCTCAGGCA AGAACTATGG GTTCACCATC CGTGCTATCC GCGTGTACGT GGGGGACAGT 
GACATCTACA CAGTGCACCA TATCGTCTGG AACGTAGAAG AAGGAAGTCC CGCATACCAG 
GCAGGACTGA AGGCCGGGGA TCTGATCACA CACATCAACG GAGAGCCGGT GCACGGCCTC 
GTCCACACGG AAGTTATCGA GCTCCTGCTG AAGAGTGGGA ATAAGGTGTC TATCACCACT 
ACTCCATTTG AAAACACATC AATCAAAACG GGACCAGCCA GGAGAAACAG TTACAAGGGC 
CGGATGGTGA GACGAAGCAA GAAGTCCAAG AAGAAGGAGA GTCTAGAAAG GAGGAGATCT 
CTCTTCAAGA AGCTGGCCAA GCAGCCTTCT CCTTTGCTCC ACACCAGCCG AAGTTTCTCC 
TGCTTAAACC GGTCCCTGTC ATCTGGAGAG AGCCTCCCGG GTTCCCCAAC TCACAGCTTG 
TCCCCGAGGT CTCCAACACC CAGTTATCGT TCTACTCCCG ATTTTCCGTC AGGTACAAAT 
TCCTCCCAGA GCAGCTCCCC AAGTTCAAGT GCCCCCAATT CTCCAGCAGG TTCAGGGCAC 
ATCCGGCCCA GCACCCTCCA TGGCCTGGCT CCCAAACTCA GCGGGCAGCG ATACCGCTCT 
GGAAGACGGA AGTCGGCTGG CAGCATCCCT CTCTCCCCGC TGGCCAGGAC ACCCTCTCCC 
ACTCCACAGC CTACCTCTCC TCAGCGTTCA CCATCCCCAC TGTTGGGACA CTCACTGGGC 
AATGCCAAGA TCACTCAGGC CTTTCCTAGC AAGATGCACT CTCCCCCCAC AATCGTCAGA 
CACATCGTGA GGCCCAAGAG TGCAGAGCCG CCCCGCTCCC CACTGCTGAA ACGGGTGCAG 
TCGGAGGAAA AGTTGTCACC CTCCTATGGC AGTGACAAGA AGCTTCTGTG CTCCCGCAAG 
CATAGCCTAG AGGTGACACA AGAGGAGGTA CAGAGGGAGC AGTGTCAGCG GGAAGTGACA 
CTGCAGAGCC TGGAAGAGAA TGTGTGTGAC GCTCCTTCCC TCAGTCGGGC CAGGCCAGTG 
GAGCAAGGCT GTCTGAAACG CCCCGTGTCC CGGAAGGTGG GCAGGCAAGA GTCTGTGGAT 
GACCTGGACC GGGACAAGCT GAAAGCCAAG GTTGTCGTAA AGAAACCAGA AGAGAAACAT 
GAATCGCACC AGAAACCTCA CAGCCTTGGT GGTGATTCGG AAAGCTATGC TCTCTTCAGG 
CTAGAGGAGA GAGAGAAAAA AGTGTACTCC AAGGGGTTGG AAAGGTCAGG CCATTTTGAA 
AACACATCAG CAGAGTTGCC TTCTGTGGGC AGCCTGCTGA AGGACACTCT TCACAAGCAG 
GCCAGCGTGA GGGCCAGCGA GGGGGTGACC TCAGACGGGG CAGCTTGCAG CCTGACACCA 
GGGGAGCACA GCCAGTCTCT AGGTGACTTT AAGCGGGCCT CAGCTTCTGG CATTCTCCAT 
GATAGTGTGT GCCCCATCTC TGATAGGCCT GCTCCTGGAA AGGTTGAATA CTCGGAGAAG 
GCCTCTCAGG CCAAAGAGCT CCTTCGAAGT GAAAAACTAG ACAGCAAGCT GGCCAATATT 
GATTACCTCA GAAAGAAAAT GTCATTGGAT GACAAAGATG ACAGCCACTG TGCCATCCTG 
AAACCCAAGA TAACATCTAG CGCCCATGAA TGTCTGCCAG GGAACCCCAT ACGGCCCATG 
GCAGGGCAAC AAGAGACCCC GCCAGCCTCT GAAAACCGGG CATTCATCAA CAGTACCCAC 
ACACCTCAGA TGAGTGCAGT TTCCTTTGTT CCTCTCAAAG CCTTAGCTGG CCGGGTAGAG 
AACGGAGGGG AGAAAGCAGG CTTAGCTGCT CCCGAGTCCC CTGTCAGGAA GAGCCCCTCC 
GAGTATAAGC TAGAGGGCAG GTCAGTTTCA TGTCTCAAGC CGATCGAGGG CACACTGGAC 
ATTGCTCTCC TGTCTGGACC TCACGCCTCC AAAACAGAGT TGCTTTCCCC AGAGCCTGCA 
CAGAGTCCCA GCCCAGGCAT CAACGTGGGA CCATGTGTGC CACTAGCTCT TCCTGGGAGC 
AGTGGGAAAA AGGGAGACTC CACCAGCCTG AGAGAGCCTT CCTCAGCCAA CTTAAAAGTA 
AATAAATCTT ATCTGCTGGA GCCTCGGTTC CTACCCCCGA GCCGGGCTCT CCAGGACTCT 
CTCGCAGCCT CTGGGCCAGA ACCGAAGTCA AAGCCGGAAA GGAAGCTCAT TCATCCTTCT 
GCCAGGAGCC CAGCAACTGT CACAGAGAGC AATCTTCAGC AGAAAGAGGG TGGTCCCGCC 
ACACACCAAG ACCGCTCCAC TGACACCAGG AACCTCCCTG GCCCAGGGCA GACCCTACAC 
AATGTGGACC TACCCAGGCT GTGTACACGT GCCCCACTCC CACCGGAAGG GACGCCCGCA 
AAGGAGAAGC CATGTCTGAA GGAACCCTCT GCCAAGGTGA AAAGCGAGTG GTCTGCCGTG 
AGGGATGACG GACACAGAGA TCCCTGTGCG AAGCTGTGCC CGGCAGAGAC TGGTAAAGCC 
AGCGACAGTT CCAAACCCCT GCCTTCCGGG GGGAGGACCC AACCCGATTT CTACAAGCAG 
ACCCAGACTT CGGAGAAAGC ATGGGCGCAT GCAAAAACAA ACCACAAAGA TAGCCAAGAT 
GAGGTGAAGT CCCTGGCCAG GGAGGACTCA GCTTCACTTT TATATGAAAA GGAGATAGGC 
CGGGCACGAA AAGGTCCTGA ACCCAAACCG GAAGTTCCTG CTACCCGGTG CCCTCCTCAG 
CCACCAGGAA TTGAGGGTGA GAAGCGAGAA AAGCTCTCCG CTGCCCCCTC TTTGCAGAAA 
CAGGCTCCCA AAGAGCCAGA CAGGAAGGAA CAGACTTCGC AAAGGCCTGG AGGTAGTGGC 
CCTCAACAAC CCCCACCCAC CAAAGAGCTG TCTAACTCAG CATCCTGGCA GCACGGCAGT 
TCTCCGAGTC ACACTTTAAA GAAGGAGCCC GGGACCAAAG CTGCCGCTGC AGAACCAAGC 
ACCAGCCTTC ATGACACTCC CCGATCTGCT ACAGCCACCA CCACTGCCAT TGCCACCACC 
ACCACTACCA CCAGTGCCGG GCACAGTGAC TGCAGTAGCC ATAAGGCCCG GCCTGGCCCT 
GACCCCAGCC CTTCAAAGTC TAAGCACCAA GACAGGTCCC TCTCCTCACA GAAGCTGAGT 
GCTGGCTCTG CAAAAGGCAA AGAGCCTGTC ACTCAACCCC TGGGTGGTTC CATCAGAGAA 
GGCAAGGGTG GCAGCAAGGG TCCAGTGGAC ACATTTTCTG CTGTCCTGAC CACCCAGGGC 
AAAGCAAGTG ATGTGCTTGT GCAGGGAGAA GGTCGGGTCT CAATCATTGT CCACACTGAA 
GAGTGTCCTC TCGATGCCAA ACTGAAAAAC ACCAATGGAG GGTGTCCCCC AGAGATGCAG 
GCGAAGCATC CACCCAGACA AGGACATCTC AGTGAAGCAG CAGACCAGAA GCCACTCATT 
GCTGGTGAGA AGCAAAGCCC GTCTCCAAAG CATCCCAAAC CATCCACTGT GAAAGATTAC 
CCCAGTCTGT GCAGACAGAC AGACAGAAGC CCAAGCCATC AGGCTACCAC TGGGGACAGG 
AAGGCAGAAG GAAAGAAATG CACAGACGCA CTTTATGTCG CAGCCCCAGA GGGCTACAAG 
CCAGAGGCCA GCCCTTCTCT CCACCACGGC GAGACCGGAC TCAGAGGCTC AGAGAGGCCA 
CCCATGGGCA TGGGGAAGGG CTTCTCTGAG CCCAAGGGGA AAGGGCCAGG TCCCCAGAAG 
TCACTGGCTG AAACAGGCAA GCCCAGCGGT ATGAAAAGGT CACCCTCTGC CACCGTGCAG 
AGCTCTCTCC GCTCAGCTGC CCCCCCAGAA AAGTCTCTGA GTTACTCAGC CAGCTTTCCC 
GAGGCCCAGC CTGGAGTGCG AGAGGTCCCT GCAGCCAACA GCAGCCCCTC ATCTGCCAAG 
GCTACAGGGG GGACCTCAGA GTTCCCAGCC CCCAGCAGCA GGGACCACAG GAAGCTTCAG 
TCTGGAGGAG ACGGCCGAAG CCAAATGATA AAGAGTGACT CTCTGCCCTC CTTCCGCCTC 
TCCACCTCTG CTCTGGAGTC ACATTTCCAG GATCCACAGG TGCCCATCGC ATCAGGCCAC 
CGAGGCAGGG CACTGTCAGT AACTGCTGCC ACAGGAGAAC CCAAAGGGAG AGAGCTCGCC 
CAGCCTCCCC CAGTCAGGAA ACAGAATGCG TGCAGAGAGG CGACCAGAGC ACCCCCAGCC 
CCAAGCACAG ATCGCTCCCT CCCTCTTTCC TCAGAGAAAG ACTTCGTGGT TCGGCAGAGA 
AGGGGCAAGG AGACCTTAAG GAGCAGTCCT CACAAAAAGG CCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_017315529.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171018.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X3, mRNA.

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CloneID	OMu49071
Clone ID Related Accession (Same CDS sequence)	XM_017315529.1
Accession Version	XM_017315529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AAGTTTCCGA GGCGCCTGAG CCCGTGCCCC GGGGCTGCAG CGGACACGGC 
GCCCGGACCC TAGTCTCTTC GGCGGCAGCC GTGTCCTCGG AGGGCGCTTC CTCAGCGGAG 
TCATCCTCTG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CCAGCCGCTT CTCCTGCAGG 
TCGCAGCCGC CGCGGCCGCC GGGCGGCGCC CTGGGAACCC GGCTACCCGC CGCGTGGGCT 
CCCGCGCGCG TGGCTCTGGA GCGTGGAGTC CCTACCCTGC CGCTGCCGCA CCCGGGAGGA 
GCGGTGCTGC CGGTGCCCCA GGTCAGCAGC GCATCCCAAG AGGAGCAGGA TGAAGAGCTT 
GACCACATAC TGTCTCCGCC ACCCATGCCG TTTCGGAAAT GCAGCAACCC AGATGTGGCC 
TGCGGCCTCG GAAAATCACT GAAGTACAAG AGACAGCTTA GTGAGGATGG GAAGCAGCTG 
CGGCGGGGGA GCCTGGGAGG AGCCCTCACA GGGAGGTACC TCCTTCCAAA CCCGGTAGCA 
GGACAGGCCT GGCCTGCTTC GGCGGAGACG TCCAACCTCG TGCGCATGCG CAGCCAGGCC 
CTTGGCCAAT CGGCTCCCTC GCTCACAGCC AGCTTGGAAG GGCAACCTCC GAAGAGCCAT 
TTCAACTCAG CTCGACATCG GCAGAGACTA GTGGACCCAG CTGCTTCAAA AAAGGAGCTG 
AGCCTCCCCC GAAGAGGAAG TCTTTGCCGA ACAAGCAACC GGAAGAGTTT GATAGGCAAT 
GGCCAGTCTC CAGCACTGCC TCGACCACAC TCACCTCTCT CTGCTCATGC AGGAAATAGC 
CCTCAAGACA GTCCAAGGAA TTTCTCCCCC AGTGCCTCAG CCCATTTCTC ATTTGCGAGG 
AGAAATGACA GAACGGATGG GCGCCGCTGG TCTCTGGCTT CTCTCCCTTC CTCAGGCTAT 
GGGACAAATA CCCCAAGCTC CACCGTCTCC TCATCCTGTT CTTCCCAGGA GAAGTTGCAC 
CAGCTACCAT ACCAGCCAAC CCCAGATGAA TTACACTTCT TATCCAAACA CTTCTGCACA 
ACAGAAAGTA TCGCCACTGA GAACCGGTGC AGAAACACAC CCATGCGTCC ACGTTCCCGG 
AGTCTCAGCC CTGGACGGTC CCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCCACA GCTCAGATGG AAGAGCGTCT GAAGGAGATC 
ATCACCAGCT ACTCTCCAGA CCATGTTCTC CCCTTGGCAG ATGGGGTACT TAGTTTCACT 
CACCATCAGA TCATTGAGCT GGCTCGGGAC TGTTTGGATA AATCTCACCA GGGTCTCATC 
ACGTCGAGAT ACTTTTTTGA GTTGCAGCAC AAACTGGACA AGTTGCTCCA GGAGGCTCAC 
GATCGCTCTG AAAGTGGAGA ACTGGCATTT ATCAAGCAAC TAGTCCGAAA GATCCTAATT 
GTCATTGCCC GCCCCGCTCG GTTATTGGAG TGTTTGGAAT TTGATCCTGA AGAATTTTAT 
TACCTATTGG AAGCTGCAGA AGGCCATGCC AAAGAAGGCC AAGGAATCAA AACTGACATC 
CCTAGGTATA TCATCAGCCA GCTGGGACTC AATAAGGACC CTTTAGAAGA AATGGCTCAG 
TTGGGGAATT ACGACAGTAG GACAGCAGAG ACACCAGAGA TGGATGAGTC AGTGAGTAGC 
TCAAATACTT CCCTGAGACT TCGAAGGAAA CCCCGAGAGA GTGATTTTGA AACAATTAAA 
TTGATCAGCA ATGGAGCCTA CGGGGCAGTC TACTTTGTTC GGCACAAAGA GTCCCGCCAG 
AGGTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTCATCC TTCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGAGACAT CCTGACTTTC GCAGAGAACC CCTTTGTGGT CAGCATGTAT 
TGCTCCTTTG AAACGAGGCG TCACTTATGC ATGGTCATGG AGTATGTAGA AGGGGGAGAC 
TGTGCGACCC TAATGAAAAA CATGGGACCT CTCCCAGTTG ATATGGCCAG AATGTATTTC 
GCCGAGACCG TCTTGGCCTT GGAGTACCTG CATAATTACG GAATCGTACA CAGGGACTTG 
AAGCCAGACA ACCTGTTGGT CACCTCCATG GGGCACATAA AACTGACTGA CTTCGGCTTG 
TCTAAGGTGG GATTAATGAG CATGACCACC AATCTCTATG AAGGCCACAT AGAGAAGGAC 
GCTCGAGAGT TCTTAGATAA ACAGGTCTGT GGTACACCTG AGTACATTGC CCCCGAGGTG 
ATTCTGAGAC AGGGCTACGG GAAACCCGTG GACTGGTGGG CCATGGGCAT TATCCTCTAT 
GAATTTCTGG TCGGATGTGT GCCTTTCTTT GGGGACACTC CAGAAGAGCT ATTTGGACAA 
GTCATCAGTG ATGAAATCAA CTGGCCTGAA AAGGACGAGG CCCCTCCTCC AGACGCTCAG 
GAGCTGATTA CCTTGCTCCT CAGGCAGAAT CCGCTGGAGA GGCTGGGAAC AGGTGGAGCC 
TATGAAGTGA AGCAGCATCG TTTCTTCCGC TCCTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCGGAAT TTATTCCCCA ACTGGAATCG GAGGATGACA CAAGTTATTT TGATACTCGG 
TCAGAGAAGT ATCATCACAT GGAGACGGAG GAGGAGGACG ATACAAACGA TGAGGACTTC 
ACCGTGGAGA TAAGGCAGTT TTCTTCCTGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGCA TAACTCAAAA TTCAGGAGAA GACAAAGATG ACTCTGAGGA CAAGACCAAA 
AGCACAACGT TGCCATCCAC AGAGACACTC AGCTGGAGTT CCGAATACTC TGAAATGCAA 
CAGTTATCGA CCTCCAACTC TTCAGATACT GAAAGCAACA GGTGCAAACT CAGCTCTGGC 
TTGCTCCCCA AGCTGGCTAT TTCGACAGAT GGGGAACAAG ATGAGGCTGT CCCTTGCTCT 
GGAGACCCCA GAGAGGAGCC AGAGAAACCT GTCCCTCCCT CTGAGGAGTG TACTCAGGAG 
GAGCCCGAGG TCACCACCCC AGCCAGCACC ATCAGCAGTT CCACACTGTC AGTTGGCAGT 
TTTTCAGAGC ACTTGGATCA GATAAATGGG CGAAGCGAGT GTGTGGACAG TACAGATAAT 
TCCTCAAAGC CATCCAGTGA ACCCACTTCT CACGTGGCTC GACAGCGCTT AGAAAGCACA 
GAGAAAAAGA AAATTTCTGG GAAAGTCACA AAGTCCCTCT CGGCCAGTGC TCTGTCCCTC 
ATGATCCCAG GAGATATGTT CGCTGTATCT CCATTGGGAA GCCCAATGTC CCCACACTCC 
CTGTCTTCAG ACCCTTCTTC TTCACGGGAT TCCTCTCCCA GCCGAGACTC TTCTGCAGCA 
TCTGCCAGTC CGCATCAGCC CATTGTCATC CACAGCTCAG GCAAGAACTA TGGGTTCACC 
ATCCGTGCTA TCCGCGTGTA CGTGGGGGAC AGTGACATCT ACACAGTGCA CCATATCGTC 
TGGAACGTAG AAGAAGGAAG TCCCGCATAC CAGGCAGGAC TGAAGGCCGG GGATCTGATC 
ACACACATCA ACGGAGAGCC GGTGCACGGC CTCGTCCACA CGGAAGTTAT CGAGCTCCTG 
CTGAAGAGTG GGAATAAGGT GTCTATCACC ACTACTCCAT TTGAAAACAC ATCAATCAAA 
ACGGGACCAG CCAGGAGAAA CAGTTACAAG GGCCGGATGG TGAGACGAAG CAAGAAGTCC 
AAGAAGAAGG AGAGTCTAGA AAGGAGGAGA TCTCTCTTCA AGAAGCTGGC CAAGCAGCCT 
TCTCCTTTGC TCCACACCAG CCGAAGTTTC TCCTGCTTAA ACCGGTCCCT GTCATCTGGA 
GAGAGCCTCC CGGGTTCCCC AACTCACAGC TTGTCCCCGA GGTCTCCAAC ACCCAGTTAT 
CGTTCTACTC CCGATTTTCC GTCAGGTACA AATTCCTCCC AGAGCAGCTC CCCAAGTTCA 
AGTGCCCCCA ATTCTCCAGC AGGTTCAGGG CACATCCGGC CCAGCACCCT CCATGGCCTG 
GCTCCCAAAC TCAGCGGGCA GCGATACCGC TCTGGAAGAC GGAAGTCGGC TGGCAGCATC 
CCTCTCTCCC CGCTGGCCAG GACACCCTCT CCCACTCCAC AGCCTACCTC TCCTCAGCGT 
TCACCATCCC CACTGTTGGG ACACTCACTG GGCAATGCCA AGATCACTCA GGCCTTTCCT 
AGCAAGATGC ACTCTCCCCC CACAATCGTC AGACACATCG TGAGGCCCAA GAGTGCAGAG 
CCGCCCCGCT CCCCACTGCT GAAACGGGTG CAGTCGGAGG AAAAGTTGTC ACCCTCCTAT 
GGCAGTGACA AGAAGCTTCT GTGCTCCCGC AAGCATAGCC TAGAGGTGAC ACAAGAGGAG 
GTACAGAGGG AGCAGTGTCA GCGGGAAGTG ACACTGCAGA GCCTGGAAGA GAATGTGTGT 
GACGCTCCTT CCCTCAGTCG GGCCAGGCCA GTGGAGCAAG GCTGTCTGAA ACGCCCCGTG 
TCCCGGAAGG TGGGCAGGCA AGAGTCTGTG GATGACCTGG ACCGGGACAA GCTGAAAGCC 
AAGGTTGTCG TAAAGAAACC AGAAGAGAAA CATGAATCGC ACCAGAAACC TCACAGCCTT 
GGTGGTGATT CGGAAAGCTA TGCTCTCTTC AGGCTAGAGG AGAGAGAGAA AAAAGTGTAC 
TCCAAGGGGT TGGAAAGGTC AGGCCATTTT GAAAACACAT CAGCAGAGTT GCCTTCTGTG 
GGCAGCCTGC TGAAGGACAC TCTTCACAAG CAGGCCAGCG TGAGGGCCAG CGAGGGGGTG 
ACCTCAGACG GGGCAGCTTG CAGCCTGACA CCAGGGGAGC ACAGCCAGTC TCTAGGTGAC 
TTTAAGCGGG CCTCAGCTTC TGGCATTCTC CATGATAGTG TGTGCCCCAT CTCTGATAGG 
CCTGCTCCTG GAAAGGTTGA ATACTCGGAG AAGGCCTCTC AGGCCAAAGA GCTCCTTCGA 
AGTGAAAAAC TAGACAGCAA GCTGGCCAAT ATTGATTACC TCAGAAAGAA AATGTCATTG 
GATGACAAAG ATGACAGCCA CTGTGCCATC CTGAAACCCA AGATAACATC TAGCGCCCAT 
GAATGTCTGC CAGGGAACCC CATACGGCCC ATGGCAGGGC AACAAGAGAC CCCGCCAGCC 
TCTGAAAACC GGGCATTCAT CAACAGTACC CACACACCTC AGATGAGTGC AGTTTCCTTT 
GTTCCTCTCA AAGCCTTAGC TGGCCGGGTA GAGAACGGAG GGGAGAAAGC AGGCTTAGCT 
GCTCCCGAGT CCCCTGTCAG GAAGAGCCCC TCCGAGTATA AGCTAGAGGG CAGGTCAGTT 
TCATGTCTCA AGCCGATCGA GGGCACACTG GACATTGCTC TCCTGTCTGG ACCTCACGCC 
TCCAAAACAG AGTTGCTTTC CCCAGAGCCT GCACAGAGTC CCAGCCCAGG CATCAACGTG 
GGACCATGTG TGCCACTAGC TCTTCCTGGG AGCAGTGGGA AAAAGGGAGA CTCCACCAGC 
CTGAGAGAGC CTTCCTCAGC CAACTTAAAA GTAAATAAAT CTTATCTGCT GGAGCCTCGG 
TTCCTACCCC CGAGCCGGGC TCTCCAGGAC TCTCTCGCAG CCTCTGGGCC AGAACCGAAG 
TCAAAGCCGG AAAGGAAGCT CATTCATCCT TCTGCCAGGA GCCCAGCAAC TGTCACAGAG 
AGCAATCTTC AGCAGAAAGA GGGTGGTCCC GCCACACACC AAGACCGCTC CACTGACACC 
AGGAACCTCC CTGGCCCAGG GCAGACCCTA CACAATGTGG ACCTACCCAG GCTGTGTACA 
CGTGCCCCAC TCCCACCGGA AGGGACGCCC GCAAAGGAGA AGCCATGTCT GAAGGAACCC 
TCTGCCAAGG TGAAAAGCGA GTGGTCTGCC GTGAGGGATG ACGGACACAG AGATCCCTGT 
GCGAAGCTGT GCCCGGCAGA GACTGGTAAA GCCAGCGACA GTTCCAAACC CCTGCCTTCC 
GGGGGGAGGA CCCAACCCGA TTTCTACAAG CAGACCCAGA CTTCGGAGAA AGCATGGGCG 
CATGCAAAAA CAAACCACAA AGATAGCCAA GATGAGGTGA AGTCCCTGGC CAGGGAGGAC 
TCAGCTTCAC TTTTATATGA AAAGGAGATA GGCCGGGCAC GAAAAGGTCC TGAACCCAAA 
CCGGAAGTTC CTGCTACCCG GTGCCCTCCT CAGCCACCAG GAATTGAGGG TGAGAAGCGA 
GAAAAGCTCT CCGCTGCCCC CTCTTTGCAG AAACAGGCTC CCAAAGAGCC AGACAGGAAG 
GAACAGACTT CGCAAAGGCC TGGAGGTAGT GGCCCTCAAC AACCCCCACC CACCAAAGAG 
CTGTCTAACT CAGCATCCTG GCAGCACGGC AGTTCTCCGA GTCACACTTT AAAGAAGGAG 
CCCGGGACCA AAGCTGCCGC TGCAGAACCA AGCACCAGCC TTCATGACAC TCCCCGATCT 
GCTACAGCCA CCACCACTGC CATTGCCACC ACCACCACTA CCACCAGTGC CGGGCACAGT 
GACTGCAGTA GCCATAAGGC CCGGCCTGGC CCTGACCCCA GCCCTTCAAA GTCTAAGCAC 
CAAGACAGGT CCCTCTCCTC ACAGAAGCTG AGTGCTGGCT CTGCAAAAGG CAAAGAGCCT 
GTCACTCAAC CCCTGGGTGG TTCCATCAGA GAAGGCAAGG GTGGCAGCAA GGGTCCAGTG 
GACACATTTT CTGCTGTCCT GACCACCCAG GGCAAAGCAA GTGATGTGCT TGTGCAGGGA 
GAAGGTCGGG TCTCAATCAT TGTCCACACT GAAGAGTGTC CTCTCGATGC CAAACTGAAA 
AACACCAATG GAGGGTGTCC CCCAGAGATG CAGGCGAAGC ATCCACCCAG ACAAGGACAT 
CTCAGTGAAG CAGCAGACCA GAAGCCACTC ATTGCTGGTG AGAAGCAAAG CCCGTCTCCA 
AAGCATCCCA AACCATCCAC TGTGAAAGAT TACCCCAGTC TGTGCAGACA GACAGACAGA 
AGCCCAAGCC ATCAGGCTAC CACTGGGGAC AGGAAGGCAG AAGGAAAGAA ATGCACAGAC 
GCACTTTATG TCGCAGCCCC AGAGGGCTAC AAGCCAGAGG CCAGCCCTTC TCTCCACCAC 
GGCGAGACCG GACTCAGAGG CTCAGAGAGG CCACCCATGG GCATGGGGAA GGGCTTCTCT 
GAGCCCAAGG GGAAAGGGCC AGGTCCCCAG AAGTCACTGG CTGAAACAGG CAAGCCCAGC 
GGTATGAAAA GGTCACCCTC TGCCACCGTG CAGAGCTCTC TCCGCTCAGC TGCCCCCCCA 
GAAAAGTCTC TGAGTTACTC AGCCAGCTTT CCCGAGGCCC AGCCTGGAGT GCGAGAGGTC 
CCTGCAGCCA ACAGCAGCCC CTCATCTGCC AAGGCTACAG GGGGGACCTC AGAGTTCCCA 
GCCCCCAGCA GCAGGGACCA CAGGAAGCTT CAGTCTGGAG GAGACGGCCG AAGCCAAATG 
ATAAAGAGTG ACTCTCTGCC CTCCTTCCGC CTCTCCACCT CTGCTCTGGA GTCACATTTC 
CAGGATCCAC AGGTGCCCAT CGCATCAGGC CACCGAGGCA GGGCACTGTC AGTAACTGCT 
GCCACAGGAG AACCCAAAGG GAGAGAGCTC GCCCAGCCTC CCCCAGTCAG GAAACAGAAT 
GCGTGCAGAG AGGCGACCAG AGCACCCCCA GCCCCAAGCA CAGATCGCTC CCTCCCTCTT 
TCCTCAGAGA AAGACTTCGT GGTTCGGCAG AGAAGGGGCA AGGAGACCTT AAGGAGCAGT 
CCTCACAAAA AGGCCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171018.1
CDS	908..8848
Translation	MGEKVSEAPEPVPRGCSGHGARTLVSSAAAVSSEGASSAESSSGSETLSEEGEPSRFSCRSQPPRPPGGALGTRLPAAWAPARVALERGVPTLPLPHPGGAVLPVPQVSSASQEEQDEELDHILSPPPMPFRKCSNPDVACGLGKSLKYKRQLSEDGKQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLEGQPPKSHFNSARHRQRLVDPAASKKELSLPRRGSLCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_017315529.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315529.1

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCCGA GGCGCCTGAG CCCGTGCCCC GGGGCTGCAG CGGACACGGC 
GCCCGGACCC TAGTCTCTTC GGCGGCAGCC GTGTCCTCGG AGGGCGCTTC CTCAGCGGAG 
TCATCCTCTG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CCAGCCGCTT CTCCTGCAGG 
TCGCAGCCGC CGCGGCCGCC GGGCGGCGCC CTGGGAACCC GGCTACCCGC CGCGTGGGCT 
CCCGCGCGCG TGGCTCTGGA GCGTGGAGTC CCTACCCTGC CGCTGCCGCA CCCGGGAGGA 
GCGGTGCTGC CGGTGCCCCA GGTCAGCAGC GCATCCCAAG AGGAGCAGGA TGAAGAGCTT 
GACCACATAC TGTCTCCGCC ACCCATGCCG TTTCGGAAAT GCAGCAACCC AGATGTGGCC 
TGCGGCCTCG GAAAATCACT GAAGTACAAG AGACAGCTTA GTGAGGATGG GAAGCAGCTG 
CGGCGGGGGA GCCTGGGAGG AGCCCTCACA GGGAGGTACC TCCTTCCAAA CCCGGTAGCA 
GGACAGGCCT GGCCTGCTTC GGCGGAGACG TCCAACCTCG TGCGCATGCG CAGCCAGGCC 
CTTGGCCAAT CGGCTCCCTC GCTCACAGCC AGCTTGGAAG GGCAACCTCC GAAGAGCCAT 
TTCAACTCAG CTCGACATCG GCAGAGACTA GTGGACCCAG CTGCTTCAAA AAAGGAGCTG 
AGCCTCCCCC GAAGAGGAAG TCTTTGCCGA ACAAGCAACC GGAAGAGTTT GATAGGCAAT 
GGCCAGTCTC CAGCACTGCC TCGACCACAC TCACCTCTCT CTGCTCATGC AGGAAATAGC 
CCTCAAGACA GTCCAAGGAA TTTCTCCCCC AGTGCCTCAG CCCATTTCTC ATTTGCGAGG 
AGAAATGACA GAACGGATGG GCGCCGCTGG TCTCTGGCTT CTCTCCCTTC CTCAGGCTAT 
GGGACAAATA CCCCAAGCTC CACCGTCTCC TCATCCTGTT CTTCCCAGGA GAAGTTGCAC 
CAGCTACCAT ACCAGCCAAC CCCAGATGAA TTACACTTCT TATCCAAACA CTTCTGCACA 
ACAGAAAGTA TCGCCACTGA GAACCGGTGC AGAAACACAC CCATGCGTCC ACGTTCCCGG 
AGTCTCAGCC CTGGACGGTC CCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCCACA GCTCAGATGG AAGAGCGTCT GAAGGAGATC 
ATCACCAGCT ACTCTCCAGA CCATGTTCTC CCCTTGGCAG ATGGGGTACT TAGTTTCACT 
CACCATCAGA TCATTGAGCT GGCTCGGGAC TGTTTGGATA AATCTCACCA GGGTCTCATC 
ACGTCGAGAT ACTTTTTTGA GTTGCAGCAC AAACTGGACA AGTTGCTCCA GGAGGCTCAC 
GATCGCTCTG AAAGTGGAGA ACTGGCATTT ATCAAGCAAC TAGTCCGAAA GATCCTAATT 
GTCATTGCCC GCCCCGCTCG GTTATTGGAG TGTTTGGAAT TTGATCCTGA AGAATTTTAT 
TACCTATTGG AAGCTGCAGA AGGCCATGCC AAAGAAGGCC AAGGAATCAA AACTGACATC 
CCTAGGTATA TCATCAGCCA GCTGGGACTC AATAAGGACC CTTTAGAAGA AATGGCTCAG 
TTGGGGAATT ACGACAGTAG GACAGCAGAG ACACCAGAGA TGGATGAGTC AGTGAGTAGC 
TCAAATACTT CCCTGAGACT TCGAAGGAAA CCCCGAGAGA GTGATTTTGA AACAATTAAA 
TTGATCAGCA ATGGAGCCTA CGGGGCAGTC TACTTTGTTC GGCACAAAGA GTCCCGCCAG 
AGGTTTGCCA TGAAGAAGAT CAACAAGCAG AACCTCATCC TTCGGAACCA GATCCAGCAG 
GCCTTCGTGG AGCGAGACAT CCTGACTTTC GCAGAGAACC CCTTTGTGGT CAGCATGTAT 
TGCTCCTTTG AAACGAGGCG TCACTTATGC ATGGTCATGG AGTATGTAGA AGGGGGAGAC 
TGTGCGACCC TAATGAAAAA CATGGGACCT CTCCCAGTTG ATATGGCCAG AATGTATTTC 
GCCGAGACCG TCTTGGCCTT GGAGTACCTG CATAATTACG GAATCGTACA CAGGGACTTG 
AAGCCAGACA ACCTGTTGGT CACCTCCATG GGGCACATAA AACTGACTGA CTTCGGCTTG 
TCTAAGGTGG GATTAATGAG CATGACCACC AATCTCTATG AAGGCCACAT AGAGAAGGAC 
GCTCGAGAGT TCTTAGATAA ACAGGTCTGT GGTACACCTG AGTACATTGC CCCCGAGGTG 
ATTCTGAGAC AGGGCTACGG GAAACCCGTG GACTGGTGGG CCATGGGCAT TATCCTCTAT 
GAATTTCTGG TCGGATGTGT GCCTTTCTTT GGGGACACTC CAGAAGAGCT ATTTGGACAA 
GTCATCAGTG ATGAAATCAA CTGGCCTGAA AAGGACGAGG CCCCTCCTCC AGACGCTCAG 
GAGCTGATTA CCTTGCTCCT CAGGCAGAAT CCGCTGGAGA GGCTGGGAAC AGGTGGAGCC 
TATGAAGTGA AGCAGCATCG TTTCTTCCGC TCCTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCGGAAT TTATTCCCCA ACTGGAATCG GAGGATGACA CAAGTTATTT TGATACTCGG 
TCAGAGAAGT ATCATCACAT GGAGACGGAG GAGGAGGACG ATACAAACGA TGAGGACTTC 
ACCGTGGAGA TAAGGCAGTT TTCTTCCTGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGCA TAACTCAAAA TTCAGGAGAA GACAAAGATG ACTCTGAGGA CAAGACCAAA 
AGCACAACGT TGCCATCCAC AGAGACACTC AGCTGGAGTT CCGAATACTC TGAAATGCAA 
CAGTTATCGA CCTCCAACTC TTCAGATACT GAAAGCAACA GGTGCAAACT CAGCTCTGGC 
TTGCTCCCCA AGCTGGCTAT TTCGACAGAT GGGGAACAAG ATGAGGCTGT CCCTTGCTCT 
GGAGACCCCA GAGAGGAGCC AGAGAAACCT GTCCCTCCCT CTGAGGAGTG TACTCAGGAG 
GAGCCCGAGG TCACCACCCC AGCCAGCACC ATCAGCAGTT CCACACTGTC AGTTGGCAGT 
TTTTCAGAGC ACTTGGATCA GATAAATGGG CGAAGCGAGT GTGTGGACAG TACAGATAAT 
TCCTCAAAGC CATCCAGTGA ACCCACTTCT CACGTGGCTC GACAGCGCTT AGAAAGCACA 
GAGAAAAAGA AAATTTCTGG GAAAGTCACA AAGTCCCTCT CGGCCAGTGC TCTGTCCCTC 
ATGATCCCAG GAGATATGTT CGCTGTATCT CCATTGGGAA GCCCAATGTC CCCACACTCC 
CTGTCTTCAG ACCCTTCTTC TTCACGGGAT TCCTCTCCCA GCCGAGACTC TTCTGCAGCA 
TCTGCCAGTC CGCATCAGCC CATTGTCATC CACAGCTCAG GCAAGAACTA TGGGTTCACC 
ATCCGTGCTA TCCGCGTGTA CGTGGGGGAC AGTGACATCT ACACAGTGCA CCATATCGTC 
TGGAACGTAG AAGAAGGAAG TCCCGCATAC CAGGCAGGAC TGAAGGCCGG GGATCTGATC 
ACACACATCA ACGGAGAGCC GGTGCACGGC CTCGTCCACA CGGAAGTTAT CGAGCTCCTG 
CTGAAGAGTG GGAATAAGGT GTCTATCACC ACTACTCCAT TTGAAAACAC ATCAATCAAA 
ACGGGACCAG CCAGGAGAAA CAGTTACAAG GGCCGGATGG TGAGACGAAG CAAGAAGTCC 
AAGAAGAAGG AGAGTCTAGA AAGGAGGAGA TCTCTCTTCA AGAAGCTGGC CAAGCAGCCT 
TCTCCTTTGC TCCACACCAG CCGAAGTTTC TCCTGCTTAA ACCGGTCCCT GTCATCTGGA 
GAGAGCCTCC CGGGTTCCCC AACTCACAGC TTGTCCCCGA GGTCTCCAAC ACCCAGTTAT 
CGTTCTACTC CCGATTTTCC GTCAGGTACA AATTCCTCCC AGAGCAGCTC CCCAAGTTCA 
AGTGCCCCCA ATTCTCCAGC AGGTTCAGGG CACATCCGGC CCAGCACCCT CCATGGCCTG 
GCTCCCAAAC TCAGCGGGCA GCGATACCGC TCTGGAAGAC GGAAGTCGGC TGGCAGCATC 
CCTCTCTCCC CGCTGGCCAG GACACCCTCT CCCACTCCAC AGCCTACCTC TCCTCAGCGT 
TCACCATCCC CACTGTTGGG ACACTCACTG GGCAATGCCA AGATCACTCA GGCCTTTCCT 
AGCAAGATGC ACTCTCCCCC CACAATCGTC AGACACATCG TGAGGCCCAA GAGTGCAGAG 
CCGCCCCGCT CCCCACTGCT GAAACGGGTG CAGTCGGAGG AAAAGTTGTC ACCCTCCTAT 
GGCAGTGACA AGAAGCTTCT GTGCTCCCGC AAGCATAGCC TAGAGGTGAC ACAAGAGGAG 
GTACAGAGGG AGCAGTGTCA GCGGGAAGTG ACACTGCAGA GCCTGGAAGA GAATGTGTGT 
GACGCTCCTT CCCTCAGTCG GGCCAGGCCA GTGGAGCAAG GCTGTCTGAA ACGCCCCGTG 
TCCCGGAAGG TGGGCAGGCA AGAGTCTGTG GATGACCTGG ACCGGGACAA GCTGAAAGCC 
AAGGTTGTCG TAAAGAAACC AGAAGAGAAA CATGAATCGC ACCAGAAACC TCACAGCCTT 
GGTGGTGATT CGGAAAGCTA TGCTCTCTTC AGGCTAGAGG AGAGAGAGAA AAAAGTGTAC 
TCCAAGGGGT TGGAAAGGTC AGGCCATTTT GAAAACACAT CAGCAGAGTT GCCTTCTGTG 
GGCAGCCTGC TGAAGGACAC TCTTCACAAG CAGGCCAGCG TGAGGGCCAG CGAGGGGGTG 
ACCTCAGACG GGGCAGCTTG CAGCCTGACA CCAGGGGAGC ACAGCCAGTC TCTAGGTGAC 
TTTAAGCGGG CCTCAGCTTC TGGCATTCTC CATGATAGTG TGTGCCCCAT CTCTGATAGG 
CCTGCTCCTG GAAAGGTTGA ATACTCGGAG AAGGCCTCTC AGGCCAAAGA GCTCCTTCGA 
AGTGAAAAAC TAGACAGCAA GCTGGCCAAT ATTGATTACC TCAGAAAGAA AATGTCATTG 
GATGACAAAG ATGACAGCCA CTGTGCCATC CTGAAACCCA AGATAACATC TAGCGCCCAT 
GAATGTCTGC CAGGGAACCC CATACGGCCC ATGGCAGGGC AACAAGAGAC CCCGCCAGCC 
TCTGAAAACC GGGCATTCAT CAACAGTACC CACACACCTC AGATGAGTGC AGTTTCCTTT 
GTTCCTCTCA AAGCCTTAGC TGGCCGGGTA GAGAACGGAG GGGAGAAAGC AGGCTTAGCT 
GCTCCCGAGT CCCCTGTCAG GAAGAGCCCC TCCGAGTATA AGCTAGAGGG CAGGTCAGTT 
TCATGTCTCA AGCCGATCGA GGGCACACTG GACATTGCTC TCCTGTCTGG ACCTCACGCC 
TCCAAAACAG AGTTGCTTTC CCCAGAGCCT GCACAGAGTC CCAGCCCAGG CATCAACGTG 
GGACCATGTG TGCCACTAGC TCTTCCTGGG AGCAGTGGGA AAAAGGGAGA CTCCACCAGC 
CTGAGAGAGC CTTCCTCAGC CAACTTAAAA GTAAATAAAT CTTATCTGCT GGAGCCTCGG 
TTCCTACCCC CGAGCCGGGC TCTCCAGGAC TCTCTCGCAG CCTCTGGGCC AGAACCGAAG 
TCAAAGCCGG AAAGGAAGCT CATTCATCCT TCTGCCAGGA GCCCAGCAAC TGTCACAGAG 
AGCAATCTTC AGCAGAAAGA GGGTGGTCCC GCCACACACC AAGACCGCTC CACTGACACC 
AGGAACCTCC CTGGCCCAGG GCAGACCCTA CACAATGTGG ACCTACCCAG GCTGTGTACA 
CGTGCCCCAC TCCCACCGGA AGGGACGCCC GCAAAGGAGA AGCCATGTCT GAAGGAACCC 
TCTGCCAAGG TGAAAAGCGA GTGGTCTGCC GTGAGGGATG ACGGACACAG AGATCCCTGT 
GCGAAGCTGT GCCCGGCAGA GACTGGTAAA GCCAGCGACA GTTCCAAACC CCTGCCTTCC 
GGGGGGAGGA CCCAACCCGA TTTCTACAAG CAGACCCAGA CTTCGGAGAA AGCATGGGCG 
CATGCAAAAA CAAACCACAA AGATAGCCAA GATGAGGTGA AGTCCCTGGC CAGGGAGGAC 
TCAGCTTCAC TTTTATATGA AAAGGAGATA GGCCGGGCAC GAAAAGGTCC TGAACCCAAA 
CCGGAAGTTC CTGCTACCCG GTGCCCTCCT CAGCCACCAG GAATTGAGGG TGAGAAGCGA 
GAAAAGCTCT CCGCTGCCCC CTCTTTGCAG AAACAGGCTC CCAAAGAGCC AGACAGGAAG 
GAACAGACTT CGCAAAGGCC TGGAGGTAGT GGCCCTCAAC AACCCCCACC CACCAAAGAG 
CTGTCTAACT CAGCATCCTG GCAGCACGGC AGTTCTCCGA GTCACACTTT AAAGAAGGAG 
CCCGGGACCA AAGCTGCCGC TGCAGAACCA AGCACCAGCC TTCATGACAC TCCCCGATCT 
GCTACAGCCA CCACCACTGC CATTGCCACC ACCACCACTA CCACCAGTGC CGGGCACAGT 
GACTGCAGTA GCCATAAGGC CCGGCCTGGC CCTGACCCCA GCCCTTCAAA GTCTAAGCAC 
CAAGACAGGT CCCTCTCCTC ACAGAAGCTG AGTGCTGGCT CTGCAAAAGG CAAAGAGCCT 
GTCACTCAAC CCCTGGGTGG TTCCATCAGA GAAGGCAAGG GTGGCAGCAA GGGTCCAGTG 
GACACATTTT CTGCTGTCCT GACCACCCAG GGCAAAGCAA GTGATGTGCT TGTGCAGGGA 
GAAGGTCGGG TCTCAATCAT TGTCCACACT GAAGAGTGTC CTCTCGATGC CAAACTGAAA 
AACACCAATG GAGGGTGTCC CCCAGAGATG CAGGCGAAGC ATCCACCCAG ACAAGGACAT 
CTCAGTGAAG CAGCAGACCA GAAGCCACTC ATTGCTGGTG AGAAGCAAAG CCCGTCTCCA 
AAGCATCCCA AACCATCCAC TGTGAAAGAT TACCCCAGTC TGTGCAGACA GACAGACAGA 
AGCCCAAGCC ATCAGGCTAC CACTGGGGAC AGGAAGGCAG AAGGAAAGAA ATGCACAGAC 
GCACTTTATG TCGCAGCCCC AGAGGGCTAC AAGCCAGAGG CCAGCCCTTC TCTCCACCAC 
GGCGAGACCG GACTCAGAGG CTCAGAGAGG CCACCCATGG GCATGGGGAA GGGCTTCTCT 
GAGCCCAAGG GGAAAGGGCC AGGTCCCCAG AAGTCACTGG CTGAAACAGG CAAGCCCAGC 
GGTATGAAAA GGTCACCCTC TGCCACCGTG CAGAGCTCTC TCCGCTCAGC TGCCCCCCCA 
GAAAAGTCTC TGAGTTACTC AGCCAGCTTT CCCGAGGCCC AGCCTGGAGT GCGAGAGGTC 
CCTGCAGCCA ACAGCAGCCC CTCATCTGCC AAGGCTACAG GGGGGACCTC AGAGTTCCCA 
GCCCCCAGCA GCAGGGACCA CAGGAAGCTT CAGTCTGGAG GAGACGGCCG AAGCCAAATG 
ATAAAGAGTG ACTCTCTGCC CTCCTTCCGC CTCTCCACCT CTGCTCTGGA GTCACATTTC 
CAGGATCCAC AGGTGCCCAT CGCATCAGGC CACCGAGGCA GGGCACTGTC AGTAACTGCT 
GCCACAGGAG AACCCAAAGG GAGAGAGCTC GCCCAGCCTC CCCCAGTCAG GAAACAGAAT 
GCGTGCAGAG AGGCGACCAG AGCACCCCCA GCCCCAAGCA CAGATCGCTC CCTCCCTCTT 
TCCTCAGAGA AAGACTTCGT GGTTCGGCAG AGAAGGGGCA AGGAGACCTT AAGGAGCAGT 
CCTCACAAAA AGGCCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_017315531.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171020.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X5, mRNA.

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CloneID	OMu49073
Clone ID Related Accession (Same CDS sequence)	XM_017315531.1
Accession Version	XM_017315531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AAGTTTCCGA GGCGCCTGAG CCCGTGCCCC GGGGCTGCAG CGGACACGGC 
GCCCGGACCC TAGTCTCTTC GGCGGCAGCC GTGTCCTCGG AGGGCGCTTC CTCAGCGGAG 
TCATCCTCTG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CCAGCCGCTT CTCCTGCAGG 
TCGCAGCCGC CGCGGCCGCC GGGCGGCGCC CTGGGAACCC GGCTACCCGC CGCGTGGGCT 
CCCGCGCGCG TGGCTCTGGA GCGTGGAGTC CCTACCCTGC CGCTGCCGCA CCCGGGAGGA 
GCGGTGCTGC CGGTGCCCCA GGTCAGCAGC GCATCCCAAG AGGAGCAGGA TGAAGAGCTT 
GACCACATAC TGTCTCCGCC ACCCATGCCG TTTCGGAAAT GCAGCAACCC AGATGTGGCC 
TGCGGCCTCG GAAAATCACT GAAGTACAAG AGACAGCTTA GTGAGGATGG GAAGCAGCTG 
CGGCGGGGGA GCCTGGGAGG AGCCCTCACA GGGAGGTACC TCCTTCCAAA CCCGGTAGCA 
GGACAGGCCT GGCCTGCTTC GGCGGAGACG TCCAACCTCG TGCGCATGCG CAGCCAGGCC 
CTTGGCCAAT CGGCTCCCTC GCTCACAGCC AGCTTGAAGG AGCTGAGCCT CCCCCGAAGA 
GGAAGTCTTT GCCGAACAAG CAACCGGAAG AGTTTGATAG GCAATGGCCA GTCTCCAGCA 
CTGCCTCGAC CACACTCACC TCTCTCTGCT CATGCAGGAA ATAGCCCTCA AGACAGTCCA 
AGGAATTTCT CCCCCAGTGC CTCAGCCCAT TTCTCATTTG CGAGGAGAAA TGACAGAACG 
GATGGGCGCC GCTGGTCTCT GGCTTCTCTC CCTTCCTCAG GCTATGGGAC AAATACCCCA 
AGCTCCACCG TCTCCTCATC CTGTTCTTCC CAGGAGAAGT TGCACCAGCT ACCATACCAG 
CCAACCCCAG ATGAATTACA CTTCTTATCC AAACACTTCT GCACAACAGA AAGTATCGCC 
ACTGAGAACC GGTGCAGAAA CACACCCATG CGTCCACGTT CCCGGAGTCT CAGCCCTGGA 
CGGTCCCCCG CCTGCTGTGA CCATGAAATA ATTATGATGA ACCATGTCTA CAAAGAAAGG 
TTCCCAAAGG CCACAGCTCA GATGGAAGAG CGTCTGAAGG AGATCATCAC CAGCTACTCT 
CCAGACCATG TTCTCCCCTT GGCAGATGGG GTACTTAGTT TCACTCACCA TCAGATCATT 
GAGCTGGCTC GGGACTGTTT GGATAAATCT CACCAGGGTC TCATCACGTC GAGATACTTT 
TTTGAGTTGC AGCACAAACT GGACAAGTTG CTCCAGGAGG CTCACGATCG CTCTGAAAGT 
GGAGAACTGG CATTTATCAA GCAACTAGTC CGAAAGATCC TAATTGTCAT TGCCCGCCCC 
GCTCGGTTAT TGGAGTGTTT GGAATTTGAT CCTGAAGAAT TTTATTACCT ATTGGAAGCT 
GCAGAAGGCC ATGCCAAAGA AGGCCAAGGA ATCAAAACTG ACATCCCTAG GTATATCATC 
AGCCAGCTGG GACTCAATAA GGACCCTTTA GAAGAAATGG CTCAGTTGGG GAATTACGAC 
AGTAGGACAG CAGAGACACC AGAGATGGAT GAGTCAGTGA GTAGCTCAAA TACTTCCCTG 
AGACTTCGAA GGAAACCCCG AGAGAGTGAT TTTGAAACAA TTAAATTGAT CAGCAATGGA 
GCCTACGGGG CAGTCTACTT TGTTCGGCAC AAAGAGTCCC GCCAGAGGTT TGCCATGAAG 
AAGATCAACA AGCAGAACCT CATCCTTCGG AACCAGATCC AGCAGGCCTT CGTGGAGCGA 
GACATCCTGA CTTTCGCAGA GAACCCCTTT GTGGTCAGCA TGTATTGCTC CTTTGAAACG 
AGGCGTCACT TATGCATGGT CATGGAGTAT GTAGAAGGGG GAGACTGTGC GACCCTAATG 
AAAAACATGG GACCTCTCCC AGTTGATATG GCCAGAATGT ATTTCGCCGA GACCGTCTTG 
GCCTTGGAGT ACCTGCATAA TTACGGAATC GTACACAGGG ACTTGAAGCC AGACAACCTG 
TTGGTCACCT CCATGGGGCA CATAAAACTG ACTGACTTCG GCTTGTCTAA GGTGGGATTA 
ATGAGCATGA CCACCAATCT CTATGAAGGC CACATAGAGA AGGACGCTCG AGAGTTCTTA 
GATAAACAGG TCTGTGGTAC ACCTGAGTAC ATTGCCCCCG AGGTGATTCT GAGACAGGGC 
TACGGGAAAC CCGTGGACTG GTGGGCCATG GGCATTATCC TCTATGAATT TCTGGTCGGA 
TGTGTGCCTT TCTTTGGGGA CACTCCAGAA GAGCTATTTG GACAAGTCAT CAGTGATGAA 
ATCAACTGGC CTGAAAAGGA CGAGGCCCCT CCTCCAGACG CTCAGGAGCT GATTACCTTG 
CTCCTCAGGC AGAATCCGCT GGAGAGGCTG GGAACAGGTG GAGCCTATGA AGTGAAGCAG 
CATCGTTTCT TCCGCTCCTT AGACTGGAAC AGTTTGCTGA GACAGAAGGC GGAATTTATT 
CCCCAACTGG AATCGGAGGA TGACACAAGT TATTTTGATA CTCGGTCAGA GAAGTATCAT 
CACATGGAGA CGGAGGAGGA GGACGATACA AACGATGAGG ACTTCACCGT GGAGATAAGG 
CAGTTTTCTT CCTGTTCACA CAGGTTTTCA AAAGTTTTCA GCAGTATAGA TCGCATAACT 
CAAAATTCAG GAGAAGACAA AGATGACTCT GAGGACAAGA CCAAAAGCAC AACGTTGCCA 
TCCACAGAGA CACTCAGCTG GAGTTCCGAA TACTCTGAAA TGCAACAGTT ATCGACCTCC 
AACTCTTCAG ATACTGAAAG CAACAGGTGC AAACTCAGCT CTGGCTTGCT CCCCAAGCTG 
GCTATTTCGA CAGATGGGGA ACAAGATGAG GCTGTCCCTT GCTCTGGAGA CCCCAGAGAG 
GAGCCAGAGA AACCTGTCCC TCCCTCTGAG GAGTGTACTC AGGAGGAGCC CGAGGTCACC 
ACCCCAGCCA GCACCATCAG CAGTTCCACA CTGTCAGTTG GCAGTTTTTC AGAGCACTTG 
GATCAGATAA ATGGGCGAAG CGAGTGTGTG GACAGTACAG ATAATTCCTC AAAGCCATCC 
AGTGAACCCA CTTCTCACGT GGCTCGACAG CGCTTAGAAA GCACAGAGAA AAAGAAAATT 
TCTGGGAAAG TCACAAAGTC CCTCTCGGCC AGTGCTCTGT CCCTCATGAT CCCAGGAGAT 
ATGTTCGCTG TATCTCCATT GGGAAGCCCA ATGTCCCCAC ACTCCCTGTC TTCAGACCCT 
TCTTCTTCAC GGGATTCCTC TCCCAGCCGA GACTCTTCTG CAGCATCTGC CAGTCCGCAT 
CAGCCCATTG TCATCCACAG CTCAGGCAAG AACTATGGGT TCACCATCCG TGCTATCCGC 
GTGTACGTGG GGGACAGTGA CATCTACACA GTGCACCATA TCGTCTGGAA CGTAGAAGAA 
GGAAGTCCCG CATACCAGGC AGGACTGAAG GCCGGGGATC TGATCACACA CATCAACGGA 
GAGCCGGTGC ACGGCCTCGT CCACACGGAA GTTATCGAGC TCCTGCTGAA GAGTGGGAAT 
AAGGTGTCTA TCACCACTAC TCCATTTGAA AACACATCAA TCAAAACGGG ACCAGCCAGG 
AGAAACAGTT ACAAGGGCCG GATGGTGAGA CGAAGCAAGA AGTCCAAGAA GAAGGAGAGT 
CTAGAAAGGA GGAGATCTCT CTTCAAGAAG CTGGCCAAGC AGCCTTCTCC TTTGCTCCAC 
ACCAGCCGAA GTTTCTCCTG CTTAAACCGG TCCCTGTCAT CTGGAGAGAG CCTCCCGGGT 
TCCCCAACTC ACAGCTTGTC CCCGAGGTCT CCAACACCCA GTTATCGTTC TACTCCCGAT 
TTTCCGTCAG GTACAAATTC CTCCCAGAGC AGCTCCCCAA GTTCAAGTGC CCCCAATTCT 
CCAGCAGGTT CAGGGCACAT CCGGCCCAGC ACCCTCCATG GCCTGGCTCC CAAACTCAGC 
GGGCAGCGAT ACCGCTCTGG AAGACGGAAG TCGGCTGGCA GCATCCCTCT CTCCCCGCTG 
GCCAGGACAC CCTCTCCCAC TCCACAGCCT ACCTCTCCTC AGCGTTCACC ATCCCCACTG 
TTGGGACACT CACTGGGCAA TGCCAAGATC ACTCAGGCCT TTCCTAGCAA GATGCACTCT 
CCCCCCACAA TCGTCAGACA CATCGTGAGG CCCAAGAGTG CAGAGCCGCC CCGCTCCCCA 
CTGCTGAAAC GGGTGCAGTC GGAGGAAAAG TTGTCACCCT CCTATGGCAG TGACAAGAAG 
CTTCTGTGCT CCCGCAAGCA TAGCCTAGAG GTGACACAAG AGGAGGTACA GAGGGAGCAG 
TGTCAGCGGG AAGTGACACT GCAGAGCCTG GAAGAGAATG TGTGTGACGC TCCTTCCCTC 
AGTCGGGCCA GGCCAGTGGA GCAAGGCTGT CTGAAACGCC CCGTGTCCCG GAAGGTGGGC 
AGGCAAGAGT CTGTGGATGA CCTGGACCGG GACAAGCTGA AAGCCAAGGT TGTCGTAAAG 
AAACCAGAAG AGAAACATGA ATCGCACCAG AAACCTCACA GCCTTGGTGG TGATTCGGAA 
AGCTATGCTC TCTTCAGGCT AGAGGAGAGA GAGAAAAAAG TGTACTCCAA GGGGTTGGAA 
AGGTCAGGCC ATTTTGAAAA CACATCAGCA GAGTTGCCTT CTGTGGGCAG CCTGCTGAAG 
GACACTCTTC ACAAGCAGGC CAGCGTGAGG GCCAGCGAGG GGGTGACCTC AGACGGGGCA 
GCTTGCAGCC TGACACCAGG GGAGCACAGC CAGTCTCTAG GTGACTTTAA GCGGGCCTCA 
GCTTCTGGCA TTCTCCATGA TAGTGTGTGC CCCATCTCTG ATAGGCCTGC TCCTGGAAAG 
GTTGAATACT CGGAGAAGGC CTCTCAGGCC AAAGAGCTCC TTCGAAGTGA AAAACTAGAC 
AGCAAGCTGG CCAATATTGA TTACCTCAGA AAGAAAATGT CATTGGATGA CAAAGATGAC 
AGCCACTGTG CCATCCTGAA ACCCAAGATA ACATCTAGCG CCCATGAATG TCTGCCAGGG 
AACCCCATAC GGCCCATGGC AGGGCAACAA GAGACCCCGC CAGCCTCTGA AAACCGGGCA 
TTCATCAACA GTACCCACAC ACCTCAGATG AGTGCAGTTT CCTTTGTTCC TCTCAAAGCC 
TTAGCTGGCC GGGTAGAGAA CGGAGGGGAG AAAGCAGGCT TAGCTGCTCC CGAGTCCCCT 
GTCAGGAAGA GCCCCTCCGA GTATAAGCTA GAGGGCAGGT CAGTTTCATG TCTCAAGCCG 
ATCGAGGGCA CACTGGACAT TGCTCTCCTG TCTGGACCTC ACGCCTCCAA AACAGAGTTG 
CTTTCCCCAG AGCCTGCACA GAGTCCCAGC CCAGGCATCA ACGTGGGACC ATGTGTGCCA 
CTAGCTCTTC CTGGGAGCAG TGGGAAAAAG GGAGACTCCA CCAGCCTGAG AGAGCCTTCC 
TCAGCCAACT TAAAAGTAAA TAAATCTTAT CTGCTGGAGC CTCGGTTCCT ACCCCCGAGC 
CGGGCTCTCC AGGACTCTCT CGCAGCCTCT GGGCCAGAAC CGAAGTCAAA GCCGGAAAGG 
AAGCTCATTC ATCCTTCTGC CAGGAGCCCA GCAACTGTCA CAGAGAGCAA TCTTCAGCAG 
AAAGAGGGTG GTCCCGCCAC ACACCAAGAC CGCTCCACTG ACACCAGGAA CCTCCCTGGC 
CCAGGGCAGA CCCTACACAA TGTGGACCTA CCCAGGCTGT GTACACGTGC CCCACTCCCA 
CCGGAAGGGA CGCCCGCAAA GGAGAAGCCA TGTCTGAAGG AACCCTCTGC CAAGGTGAAA 
AGCGAGTGGT CTGCCGTGAG GGATGACGGA CACAGAGATC CCTGTGCGAA GCTGTGCCCG 
GCAGAGACTG GTAAAGCCAG CGACAGTTCC AAACCCCTGC CTTCCGGGGG GAGGACCCAA 
CCCGATTTCT ACAAGCAGAC CCAGACTTCG GAGAAAGCAT GGGCGCATGC AAAAACAAAC 
CACAAAGATA GCCAAGATGA GGTGAAGTCC CTGGCCAGGG AGGACTCAGC TTCACTTTTA 
TATGAAAAGG AGATAGGCCG GGCACGAAAA GGTCCTGAAC CCAAACCGGA AGTTCCTGCT 
ACCCGGTGCC CTCCTCAGCC ACCAGGAATT GAGGGTGAGA AGCGAGAAAA GCTCTCCGCT 
GCCCCCTCTT TGCAGAAACA GGCTCCCAAA GAGCCAGACA GGAAGGAACA GACTTCGCAA 
AGGCCTGGAG GTAGTGGCCC TCAACAACCC CCACCCACCA AAGAGCTGTC TAACTCAGCA 
TCCTGGCAGC ACGGCAGTTC TCCGAGTCAC ACTTTAAAGA AGGAGCCCGG GACCAAAGCT 
GCCGCTGCAG AACCAAGCAC CAGCCTTCAT GACACTCCCC GATCTGCTAC AGCCACCACC 
ACTGCCATTG CCACCACCAC CACTACCACC AGTGCCGGGC ACAGTGACTG CAGTAGCCAT 
AAGGCCCGGC CTGGCCCTGA CCCCAGCCCT TCAAAGTCTA AGCACCAAGA CAGGTCCCTC 
TCCTCACAGA AGCTGAGTGC TGGCTCTGCA AAAGGCAAAG AGCCTGTCAC TCAACCCCTG 
GGTGGTTCCA TCAGAGAAGG CAAGGGTGGC AGCAAGGGTC CAGTGGACAC ATTTTCTGCT 
GTCCTGACCA CCCAGGGCAA AGCAAGTGAT GTGCTTGTGC AGGGAGAAGG TCGGGTCTCA 
ATCATTGTCC ACACTGAAGA GTGTCCTCTC GATGCCAAAC TGAAAAACAC CAATGGAGGG 
TGTCCCCCAG AGATGCAGGC GAAGCATCCA CCCAGACAAG GACATCTCAG TGAAGCAGCA 
GACCAGAAGC CACTCATTGC TGGTGAGAAG CAAAGCCCGT CTCCAAAGCA TCCCAAACCA 
TCCACTGTGA AAGATTACCC CAGTCTGTGC AGACAGACAG ACAGAAGCCC AAGCCATCAG 
GCTACCACTG GGGACAGGAA GGCAGAAGGA AAGAAATGCA CAGACGCACT TTATGTCGCA 
GCCCCAGAGG GCTACAAGCC AGAGGCCAGC CCTTCTCTCC ACCACGGCGA GACCGGACTC 
AGAGGCTCAG AGAGGCCACC CATGGGCATG GGGAAGGGCT TCTCTGAGCC CAAGGGGAAA 
GGGCCAGGTC CCCAGAAGTC ACTGGCTGAA ACAGGCAAGC CCAGCGGTAT GAAAAGGTCA 
CCCTCTGCCA CCGTGCAGAG CTCTCTCCGC TCAGCTGCCC CCCCAGAAAA GTCTCTGAGT 
TACTCAGCCA GCTTTCCCGA GGCCCAGCCT GGAGTGCGAG AGGTCCCTGC AGCCAACAGC 
AGCCCCTCAT CTGCCAAGGC TACAGGGGGG ACCTCAGAGT TCCCAGCCCC CAGCAGCAGG 
GACCACAGGA AGCTTCAGTC TGGAGGAGAC GGCCGAAGCC AAATGATAAA GAGTGACTCT 
CTGCCCTCCT TCCGCCTCTC CACCTCTGCT CTGGAGTCAC ATTTCCAGGA TCCACAGGTG 
CCCATCGCAT CAGGCCACCG AGGCAGGGCA CTGTCAGTAA CTGCTGCCAC AGGAGAACCC 
AAAGGGAGAG AGCTCGCCCA GCCTCCCCCA GTCAGGAAAC AGAATGCGTG CAGAGAGGCG 
ACCAGAGCAC CCCCAGCCCC AAGCACAGAT CGCTCCCTCC CTCTTTCCTC AGAGAAAGAC 
TTCGTGGTTC GGCAGAGAAG GGGCAAGGAG ACCTTAAGGA GCAGTCCTCA CAAAAAGGCC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171020.1
CDS	908..8773
Translation	MGEKVSEAPEPVPRGCSGHGARTLVSSAAAVSSEGASSAESSSGSETLSEEGEPSRFSCRSQPPRPPGGALGTRLPAAWAPARVALERGVPTLPLPHPGGAVLPVPQVSSASQEEQDEELDHILSPPPMPFRKCSNPDVACGLGKSLKYKRQLSEDGKQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLKELSLPRRGSLCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_017315531.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315531.1

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCCGA GGCGCCTGAG CCCGTGCCCC GGGGCTGCAG CGGACACGGC 
GCCCGGACCC TAGTCTCTTC GGCGGCAGCC GTGTCCTCGG AGGGCGCTTC CTCAGCGGAG 
TCATCCTCTG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CCAGCCGCTT CTCCTGCAGG 
TCGCAGCCGC CGCGGCCGCC GGGCGGCGCC CTGGGAACCC GGCTACCCGC CGCGTGGGCT 
CCCGCGCGCG TGGCTCTGGA GCGTGGAGTC CCTACCCTGC CGCTGCCGCA CCCGGGAGGA 
GCGGTGCTGC CGGTGCCCCA GGTCAGCAGC GCATCCCAAG AGGAGCAGGA TGAAGAGCTT 
GACCACATAC TGTCTCCGCC ACCCATGCCG TTTCGGAAAT GCAGCAACCC AGATGTGGCC 
TGCGGCCTCG GAAAATCACT GAAGTACAAG AGACAGCTTA GTGAGGATGG GAAGCAGCTG 
CGGCGGGGGA GCCTGGGAGG AGCCCTCACA GGGAGGTACC TCCTTCCAAA CCCGGTAGCA 
GGACAGGCCT GGCCTGCTTC GGCGGAGACG TCCAACCTCG TGCGCATGCG CAGCCAGGCC 
CTTGGCCAAT CGGCTCCCTC GCTCACAGCC AGCTTGAAGG AGCTGAGCCT CCCCCGAAGA 
GGAAGTCTTT GCCGAACAAG CAACCGGAAG AGTTTGATAG GCAATGGCCA GTCTCCAGCA 
CTGCCTCGAC CACACTCACC TCTCTCTGCT CATGCAGGAA ATAGCCCTCA AGACAGTCCA 
AGGAATTTCT CCCCCAGTGC CTCAGCCCAT TTCTCATTTG CGAGGAGAAA TGACAGAACG 
GATGGGCGCC GCTGGTCTCT GGCTTCTCTC CCTTCCTCAG GCTATGGGAC AAATACCCCA 
AGCTCCACCG TCTCCTCATC CTGTTCTTCC CAGGAGAAGT TGCACCAGCT ACCATACCAG 
CCAACCCCAG ATGAATTACA CTTCTTATCC AAACACTTCT GCACAACAGA AAGTATCGCC 
ACTGAGAACC GGTGCAGAAA CACACCCATG CGTCCACGTT CCCGGAGTCT CAGCCCTGGA 
CGGTCCCCCG CCTGCTGTGA CCATGAAATA ATTATGATGA ACCATGTCTA CAAAGAAAGG 
TTCCCAAAGG CCACAGCTCA GATGGAAGAG CGTCTGAAGG AGATCATCAC CAGCTACTCT 
CCAGACCATG TTCTCCCCTT GGCAGATGGG GTACTTAGTT TCACTCACCA TCAGATCATT 
GAGCTGGCTC GGGACTGTTT GGATAAATCT CACCAGGGTC TCATCACGTC GAGATACTTT 
TTTGAGTTGC AGCACAAACT GGACAAGTTG CTCCAGGAGG CTCACGATCG CTCTGAAAGT 
GGAGAACTGG CATTTATCAA GCAACTAGTC CGAAAGATCC TAATTGTCAT TGCCCGCCCC 
GCTCGGTTAT TGGAGTGTTT GGAATTTGAT CCTGAAGAAT TTTATTACCT ATTGGAAGCT 
GCAGAAGGCC ATGCCAAAGA AGGCCAAGGA ATCAAAACTG ACATCCCTAG GTATATCATC 
AGCCAGCTGG GACTCAATAA GGACCCTTTA GAAGAAATGG CTCAGTTGGG GAATTACGAC 
AGTAGGACAG CAGAGACACC AGAGATGGAT GAGTCAGTGA GTAGCTCAAA TACTTCCCTG 
AGACTTCGAA GGAAACCCCG AGAGAGTGAT TTTGAAACAA TTAAATTGAT CAGCAATGGA 
GCCTACGGGG CAGTCTACTT TGTTCGGCAC AAAGAGTCCC GCCAGAGGTT TGCCATGAAG 
AAGATCAACA AGCAGAACCT CATCCTTCGG AACCAGATCC AGCAGGCCTT CGTGGAGCGA 
GACATCCTGA CTTTCGCAGA GAACCCCTTT GTGGTCAGCA TGTATTGCTC CTTTGAAACG 
AGGCGTCACT TATGCATGGT CATGGAGTAT GTAGAAGGGG GAGACTGTGC GACCCTAATG 
AAAAACATGG GACCTCTCCC AGTTGATATG GCCAGAATGT ATTTCGCCGA GACCGTCTTG 
GCCTTGGAGT ACCTGCATAA TTACGGAATC GTACACAGGG ACTTGAAGCC AGACAACCTG 
TTGGTCACCT CCATGGGGCA CATAAAACTG ACTGACTTCG GCTTGTCTAA GGTGGGATTA 
ATGAGCATGA CCACCAATCT CTATGAAGGC CACATAGAGA AGGACGCTCG AGAGTTCTTA 
GATAAACAGG TCTGTGGTAC ACCTGAGTAC ATTGCCCCCG AGGTGATTCT GAGACAGGGC 
TACGGGAAAC CCGTGGACTG GTGGGCCATG GGCATTATCC TCTATGAATT TCTGGTCGGA 
TGTGTGCCTT TCTTTGGGGA CACTCCAGAA GAGCTATTTG GACAAGTCAT CAGTGATGAA 
ATCAACTGGC CTGAAAAGGA CGAGGCCCCT CCTCCAGACG CTCAGGAGCT GATTACCTTG 
CTCCTCAGGC AGAATCCGCT GGAGAGGCTG GGAACAGGTG GAGCCTATGA AGTGAAGCAG 
CATCGTTTCT TCCGCTCCTT AGACTGGAAC AGTTTGCTGA GACAGAAGGC GGAATTTATT 
CCCCAACTGG AATCGGAGGA TGACACAAGT TATTTTGATA CTCGGTCAGA GAAGTATCAT 
CACATGGAGA CGGAGGAGGA GGACGATACA AACGATGAGG ACTTCACCGT GGAGATAAGG 
CAGTTTTCTT CCTGTTCACA CAGGTTTTCA AAAGTTTTCA GCAGTATAGA TCGCATAACT 
CAAAATTCAG GAGAAGACAA AGATGACTCT GAGGACAAGA CCAAAAGCAC AACGTTGCCA 
TCCACAGAGA CACTCAGCTG GAGTTCCGAA TACTCTGAAA TGCAACAGTT ATCGACCTCC 
AACTCTTCAG ATACTGAAAG CAACAGGTGC AAACTCAGCT CTGGCTTGCT CCCCAAGCTG 
GCTATTTCGA CAGATGGGGA ACAAGATGAG GCTGTCCCTT GCTCTGGAGA CCCCAGAGAG 
GAGCCAGAGA AACCTGTCCC TCCCTCTGAG GAGTGTACTC AGGAGGAGCC CGAGGTCACC 
ACCCCAGCCA GCACCATCAG CAGTTCCACA CTGTCAGTTG GCAGTTTTTC AGAGCACTTG 
GATCAGATAA ATGGGCGAAG CGAGTGTGTG GACAGTACAG ATAATTCCTC AAAGCCATCC 
AGTGAACCCA CTTCTCACGT GGCTCGACAG CGCTTAGAAA GCACAGAGAA AAAGAAAATT 
TCTGGGAAAG TCACAAAGTC CCTCTCGGCC AGTGCTCTGT CCCTCATGAT CCCAGGAGAT 
ATGTTCGCTG TATCTCCATT GGGAAGCCCA ATGTCCCCAC ACTCCCTGTC TTCAGACCCT 
TCTTCTTCAC GGGATTCCTC TCCCAGCCGA GACTCTTCTG CAGCATCTGC CAGTCCGCAT 
CAGCCCATTG TCATCCACAG CTCAGGCAAG AACTATGGGT TCACCATCCG TGCTATCCGC 
GTGTACGTGG GGGACAGTGA CATCTACACA GTGCACCATA TCGTCTGGAA CGTAGAAGAA 
GGAAGTCCCG CATACCAGGC AGGACTGAAG GCCGGGGATC TGATCACACA CATCAACGGA 
GAGCCGGTGC ACGGCCTCGT CCACACGGAA GTTATCGAGC TCCTGCTGAA GAGTGGGAAT 
AAGGTGTCTA TCACCACTAC TCCATTTGAA AACACATCAA TCAAAACGGG ACCAGCCAGG 
AGAAACAGTT ACAAGGGCCG GATGGTGAGA CGAAGCAAGA AGTCCAAGAA GAAGGAGAGT 
CTAGAAAGGA GGAGATCTCT CTTCAAGAAG CTGGCCAAGC AGCCTTCTCC TTTGCTCCAC 
ACCAGCCGAA GTTTCTCCTG CTTAAACCGG TCCCTGTCAT CTGGAGAGAG CCTCCCGGGT 
TCCCCAACTC ACAGCTTGTC CCCGAGGTCT CCAACACCCA GTTATCGTTC TACTCCCGAT 
TTTCCGTCAG GTACAAATTC CTCCCAGAGC AGCTCCCCAA GTTCAAGTGC CCCCAATTCT 
CCAGCAGGTT CAGGGCACAT CCGGCCCAGC ACCCTCCATG GCCTGGCTCC CAAACTCAGC 
GGGCAGCGAT ACCGCTCTGG AAGACGGAAG TCGGCTGGCA GCATCCCTCT CTCCCCGCTG 
GCCAGGACAC CCTCTCCCAC TCCACAGCCT ACCTCTCCTC AGCGTTCACC ATCCCCACTG 
TTGGGACACT CACTGGGCAA TGCCAAGATC ACTCAGGCCT TTCCTAGCAA GATGCACTCT 
CCCCCCACAA TCGTCAGACA CATCGTGAGG CCCAAGAGTG CAGAGCCGCC CCGCTCCCCA 
CTGCTGAAAC GGGTGCAGTC GGAGGAAAAG TTGTCACCCT CCTATGGCAG TGACAAGAAG 
CTTCTGTGCT CCCGCAAGCA TAGCCTAGAG GTGACACAAG AGGAGGTACA GAGGGAGCAG 
TGTCAGCGGG AAGTGACACT GCAGAGCCTG GAAGAGAATG TGTGTGACGC TCCTTCCCTC 
AGTCGGGCCA GGCCAGTGGA GCAAGGCTGT CTGAAACGCC CCGTGTCCCG GAAGGTGGGC 
AGGCAAGAGT CTGTGGATGA CCTGGACCGG GACAAGCTGA AAGCCAAGGT TGTCGTAAAG 
AAACCAGAAG AGAAACATGA ATCGCACCAG AAACCTCACA GCCTTGGTGG TGATTCGGAA 
AGCTATGCTC TCTTCAGGCT AGAGGAGAGA GAGAAAAAAG TGTACTCCAA GGGGTTGGAA 
AGGTCAGGCC ATTTTGAAAA CACATCAGCA GAGTTGCCTT CTGTGGGCAG CCTGCTGAAG 
GACACTCTTC ACAAGCAGGC CAGCGTGAGG GCCAGCGAGG GGGTGACCTC AGACGGGGCA 
GCTTGCAGCC TGACACCAGG GGAGCACAGC CAGTCTCTAG GTGACTTTAA GCGGGCCTCA 
GCTTCTGGCA TTCTCCATGA TAGTGTGTGC CCCATCTCTG ATAGGCCTGC TCCTGGAAAG 
GTTGAATACT CGGAGAAGGC CTCTCAGGCC AAAGAGCTCC TTCGAAGTGA AAAACTAGAC 
AGCAAGCTGG CCAATATTGA TTACCTCAGA AAGAAAATGT CATTGGATGA CAAAGATGAC 
AGCCACTGTG CCATCCTGAA ACCCAAGATA ACATCTAGCG CCCATGAATG TCTGCCAGGG 
AACCCCATAC GGCCCATGGC AGGGCAACAA GAGACCCCGC CAGCCTCTGA AAACCGGGCA 
TTCATCAACA GTACCCACAC ACCTCAGATG AGTGCAGTTT CCTTTGTTCC TCTCAAAGCC 
TTAGCTGGCC GGGTAGAGAA CGGAGGGGAG AAAGCAGGCT TAGCTGCTCC CGAGTCCCCT 
GTCAGGAAGA GCCCCTCCGA GTATAAGCTA GAGGGCAGGT CAGTTTCATG TCTCAAGCCG 
ATCGAGGGCA CACTGGACAT TGCTCTCCTG TCTGGACCTC ACGCCTCCAA AACAGAGTTG 
CTTTCCCCAG AGCCTGCACA GAGTCCCAGC CCAGGCATCA ACGTGGGACC ATGTGTGCCA 
CTAGCTCTTC CTGGGAGCAG TGGGAAAAAG GGAGACTCCA CCAGCCTGAG AGAGCCTTCC 
TCAGCCAACT TAAAAGTAAA TAAATCTTAT CTGCTGGAGC CTCGGTTCCT ACCCCCGAGC 
CGGGCTCTCC AGGACTCTCT CGCAGCCTCT GGGCCAGAAC CGAAGTCAAA GCCGGAAAGG 
AAGCTCATTC ATCCTTCTGC CAGGAGCCCA GCAACTGTCA CAGAGAGCAA TCTTCAGCAG 
AAAGAGGGTG GTCCCGCCAC ACACCAAGAC CGCTCCACTG ACACCAGGAA CCTCCCTGGC 
CCAGGGCAGA CCCTACACAA TGTGGACCTA CCCAGGCTGT GTACACGTGC CCCACTCCCA 
CCGGAAGGGA CGCCCGCAAA GGAGAAGCCA TGTCTGAAGG AACCCTCTGC CAAGGTGAAA 
AGCGAGTGGT CTGCCGTGAG GGATGACGGA CACAGAGATC CCTGTGCGAA GCTGTGCCCG 
GCAGAGACTG GTAAAGCCAG CGACAGTTCC AAACCCCTGC CTTCCGGGGG GAGGACCCAA 
CCCGATTTCT ACAAGCAGAC CCAGACTTCG GAGAAAGCAT GGGCGCATGC AAAAACAAAC 
CACAAAGATA GCCAAGATGA GGTGAAGTCC CTGGCCAGGG AGGACTCAGC TTCACTTTTA 
TATGAAAAGG AGATAGGCCG GGCACGAAAA GGTCCTGAAC CCAAACCGGA AGTTCCTGCT 
ACCCGGTGCC CTCCTCAGCC ACCAGGAATT GAGGGTGAGA AGCGAGAAAA GCTCTCCGCT 
GCCCCCTCTT TGCAGAAACA GGCTCCCAAA GAGCCAGACA GGAAGGAACA GACTTCGCAA 
AGGCCTGGAG GTAGTGGCCC TCAACAACCC CCACCCACCA AAGAGCTGTC TAACTCAGCA 
TCCTGGCAGC ACGGCAGTTC TCCGAGTCAC ACTTTAAAGA AGGAGCCCGG GACCAAAGCT 
GCCGCTGCAG AACCAAGCAC CAGCCTTCAT GACACTCCCC GATCTGCTAC AGCCACCACC 
ACTGCCATTG CCACCACCAC CACTACCACC AGTGCCGGGC ACAGTGACTG CAGTAGCCAT 
AAGGCCCGGC CTGGCCCTGA CCCCAGCCCT TCAAAGTCTA AGCACCAAGA CAGGTCCCTC 
TCCTCACAGA AGCTGAGTGC TGGCTCTGCA AAAGGCAAAG AGCCTGTCAC TCAACCCCTG 
GGTGGTTCCA TCAGAGAAGG CAAGGGTGGC AGCAAGGGTC CAGTGGACAC ATTTTCTGCT 
GTCCTGACCA CCCAGGGCAA AGCAAGTGAT GTGCTTGTGC AGGGAGAAGG TCGGGTCTCA 
ATCATTGTCC ACACTGAAGA GTGTCCTCTC GATGCCAAAC TGAAAAACAC CAATGGAGGG 
TGTCCCCCAG AGATGCAGGC GAAGCATCCA CCCAGACAAG GACATCTCAG TGAAGCAGCA 
GACCAGAAGC CACTCATTGC TGGTGAGAAG CAAAGCCCGT CTCCAAAGCA TCCCAAACCA 
TCCACTGTGA AAGATTACCC CAGTCTGTGC AGACAGACAG ACAGAAGCCC AAGCCATCAG 
GCTACCACTG GGGACAGGAA GGCAGAAGGA AAGAAATGCA CAGACGCACT TTATGTCGCA 
GCCCCAGAGG GCTACAAGCC AGAGGCCAGC CCTTCTCTCC ACCACGGCGA GACCGGACTC 
AGAGGCTCAG AGAGGCCACC CATGGGCATG GGGAAGGGCT TCTCTGAGCC CAAGGGGAAA 
GGGCCAGGTC CCCAGAAGTC ACTGGCTGAA ACAGGCAAGC CCAGCGGTAT GAAAAGGTCA 
CCCTCTGCCA CCGTGCAGAG CTCTCTCCGC TCAGCTGCCC CCCCAGAAAA GTCTCTGAGT 
TACTCAGCCA GCTTTCCCGA GGCCCAGCCT GGAGTGCGAG AGGTCCCTGC AGCCAACAGC 
AGCCCCTCAT CTGCCAAGGC TACAGGGGGG ACCTCAGAGT TCCCAGCCCC CAGCAGCAGG 
GACCACAGGA AGCTTCAGTC TGGAGGAGAC GGCCGAAGCC AAATGATAAA GAGTGACTCT 
CTGCCCTCCT TCCGCCTCTC CACCTCTGCT CTGGAGTCAC ATTTCCAGGA TCCACAGGTG 
CCCATCGCAT CAGGCCACCG AGGCAGGGCA CTGTCAGTAA CTGCTGCCAC AGGAGAACCC 
AAAGGGAGAG AGCTCGCCCA GCCTCCCCCA GTCAGGAAAC AGAATGCGTG CAGAGAGGCG 
ACCAGAGCAC CCCCAGCCCC AAGCACAGAT CGCTCCCTCC CTCTTTCCTC AGAGAAAGAC 
TTCGTGGTTC GGCAGAGAAG GGGCAAGGAG ACCTTAAGGA GCAGTCCTCA CAAAAAGGCC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_017315532.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171021.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X6, mRNA.

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CloneID	OMu49074
Clone ID Related Accession (Same CDS sequence)	XM_017315532.1
Accession Version	XM_017315532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AAGTTTCCGA GGCGCCTGAG CCCGTGCCCC GGGGCTGCAG CGGACACGGC 
GCCCGGACCC TAGTCTCTTC GGCGGCAGCC GTGTCCTCGG AGGGCGCTTC CTCAGCGGAG 
TCATCCTCTG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CCAGCCGCTT CTCCTGCAGG 
TCGCAGCCGC CGCGGCCGCC GGGCGGCGCC CTGGGAACCC GGCTACCCGC CGCGTGGGCT 
CCCGCGCGCG TGGCTCTGGA GCGTGGAGTC CCTACCCTGC CGCTGCCGCA CCCGGGAGGA 
GCGGTGCTGC CGGTGCCCCA GGTCAGCAGC GCATCCCAAG AGGAGCAGGA TGAAGAGCTT 
GACCACATAC TGTCTCCGCC ACCCATGCCG TTTCGGAAAT GCAGCAACCC AGATGTGGCC 
TGCGGCCTCG GAAAATCACT GAAGTACAAG AGACAGCTTA GTGAGGATGG GAAGCAGCTG 
CGGCGGGGGA GCCTGGGAGG AGCCCTCACA GGGAGGTACC TCCTTCCAAA CCCGGTAGCA 
GGACAGGCCT GGCCTGCTTC GGCGGAGACG TCCAACCTCG TGCGCATGCG CAGCCAGGCC 
CTTGGCCAAT CGGCTCCCTC GCTCACAGCC AGCTTGGAAG GGCAACCTCC GAAGAGCCAT 
TTCAACTCAG CTCGACATCG GCAGAGACTA GTGGACCCAG CTGCTTCAAA AAAGGAGCTG 
AGCCTCCCCC GAAGAGGAAG TCTGGTAGAT TCCCAGAAGT GGAATTGCTT GGTCAAACGT 
TGCCGAACAA GCAACCGGAA GAGTTTGATA GGCAATGGCC AGTCTCCAGC ACTGCCTCGA 
CCACACTCAC CTCTCTCTGC TCATGCAGGA AATAGCCCTC AAGACAGTCC AAGGAATTTC 
TCCCCCAGTG CCTCAGCCCA TTTCTCATTT GCGAGGAGAA ATGACAGAAC GGATGGGCGC 
CGCTGGTCTC TGGCTTCTCT CCCTTCCTCA GGCTATGGGA CAAATACCCC AAGCTCCACC 
GTCTCCTCAT CCTGTTCTTC CCAGGAGAAG TTGCACCAGC TACCATACCA GCCAACCCCA 
GATGAATTAC ACTTCTTATC CAAACACTTC TGCACAACAG AAAGTATCGC CACTGAGAAC 
CGGTGCAGAA ACACACCCAT GCGTCCACGT TCCCGGAGTC TCAGCCCTGG ACGGTCCCCC 
GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG 
GCCACAGCTC AGATGGAAGA GCGTCTGAAG GAGATCATCA CCAGCTACTC TCCAGACCAT 
GTTCTCCCCT TGGCAGATGG GGTACTTAGT TTCACTCACC ATCAGATCAT TGAGCTGGCT 
CGGGACTGTT TGGATAAATC TCACCAGGGT CTCATCACGT CGAGATACTT TTTTGAGTTG 
CAGCACAAAC TGGACAAGTT GCTCCAGGAG GCTCACGATC GCTCTGAAAG TGGAGAACTG 
GCATTTATCA AGCAACTAGT CCGAAAGATC CTAATTGTCA TTGCCCGCCC CGCTCGGTTA 
TTGGAGTGTT TGGAATTTGA TCCTGAAGAA TTTTATTACC TATTGGAAGC TGCAGAAGGC 
CATGCCAAAG AAGGCCAAGG AATCAAAACT GACATCCCTA GGTATATCAT CAGCCAGCTG 
GGACTCAATA AGGACCCTTT AGAAGAAATG GCTCAGTTGG GGAATTACGA CAGTAGGACA 
GCAGAGACAC CAGAGATGGA TGAGTCAGTG AGTAGCTCAA ATACTTCCCT GAGACTTCGA 
AGGAAACCCC GAGAGAGTGA TTTTGAAACA ATTAAATTGA TCAGCAATGG AGCCTACGGG 
GCAGTCTACT TTGTTCGGCA CAAAGAGTCC CGCCAGAGGT TTGCCATGAA GAAGATCAAC 
AAGCAGAACC TCATCCTTCG GAACCAGATC CAGCAGGCCT TCGTGGAGCG AGACATCCTG 
ACTTTCGCAG AGAACCCCTT TGTGGTCAGC ATGTATTGCT CCTTTGAAAC GAGGCGTCAC 
TTATGCATGG TCATGGAGTA TGTAGAAGGG GGAGACTGTG CGACCCTAAT GAAAAACATG 
GGACCTCTCC CAGTTGATAT GGCCAGAATG TATTTCGCCG AGACCGTCTT GGCCTTGGAG 
TACCTGCATA ATTACGGAAT CGTACACAGG GACTTGAAGC CAGACAACCT GTTGGTCACC 
TCCATGGGGC ACATAAAACT GACTGACTTC GGCTTGTCTA AGGTGGGATT AATGAGCATG 
ACCACCAATC TCTATGAAGG CCACATAGAG AAGGACGCTC GAGAGTTCTT AGATAAACAG 
GTCTGTGGTA CACCTGAGTA CATTGCCCCC GAGGTGATTC TGAGACAGGG CTACGGGAAA 
CCCGTGGACT GGTGGGCCAT GGGCATTATC CTCTATGAAT TTCTGGTCGG ATGTGTGCCT 
TTCTTTGGGG ACACTCCAGA AGAGCTATTT GGACAAGTCA TCAGTGATGA AATCAACTGG 
CCTGAAAAGG ACGAGGCCCC TCCTCCAGAC GCTCAGGAGC TGATTACCTT GCTCCTCAGG 
CAGAATCCGC TGGAGAGGCT GGGAACAGGT GGAGCCTATG AAGTGAAGCA GCATCGTTTC 
TTCCGCTCCT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CGGAATTTAT TCCCCAACTG 
GAATCGGAGG ATGACACAAG TTATTTTGAT ACTCGGTCAG AGAAGTATCA TCACATGGAG 
ACGGAGGAGG AGGACGATAC AAACGATGAG GACTTCACCG TGGAGATAAG GCAGTTTTCT 
TCCTGTTCAC ACAGGTTTTC AAAAGTTTTC AGCAGTATAG ATCGCATAAC TCAAAATTCA 
GGAGAAGACA AAGATGACTC TGAGGACAAG ACCAAAAGCA CAACGTTGCC ATCCACAGAG 
ACACTCAGCT GGAGTTCCGA ATACTCTGAA ATGCAACAGT TATCGACCTC CAACTCTTCA 
GATACTGAAA GCAACAGGTG CAAACTCAGC TCTGGCTTGC TCCCCAAGCT GGCTATTTCG 
ACAGATGGGG AACAAGATGA GGCTGTCCCT TGCTCTGGAG ACCCCAGAGA GGAGCCAGAG 
AAACCTGTCC CTCCCTCTGA GGAGTGTACT CAGGAGGAGC CCGAGGTCAC CACCCCAGCC 
AGCACCATCA GCAGTTCCAC ACTGTCAGAT ATGTTCGCTG TATCTCCATT GGGAAGCCCA 
ATGTCCCCAC ACTCCCTGTC TTCAGACCCT TCTTCTTCAC GGGATTCCTC TCCCAGCCGA 
GACTCTTCTG CAGCATCTGC CAGTCCGCAT CAGCCCATTG TCATCCACAG CTCAGGCAAG 
AACTATGGGT TCACCATCCG TGCTATCCGC GTGTACGTGG GGGACAGTGA CATCTACACA 
GTGCACCATA TCGTCTGGAA CGTAGAAGAA GGAAGTCCCG CATACCAGGC AGGACTGAAG 
GCCGGGGATC TGATCACACA CATCAACGGA GAGCCGGTGC ACGGCCTCGT CCACACGGAA 
GTTATCGAGC TCCTGCTGAA GAGTGGGAAT AAGGTGTCTA TCACCACTAC TCCATTTGAA 
AACACATCAA TCAAAACGGG ACCAGCCAGG AGAAACAGTT ACAAGGGCCG GATGGTGAGA 
CGAAGCAAGA AGTCCAAGAA GAAGGAGAGT CTAGAAAGGA GGAGATCTCT CTTCAAGAAG 
CTGGCCAAGC AGCCTTCTCC TTTGCTCCAC ACCAGCCGAA GTTTCTCCTG CTTAAACCGG 
TCCCTGTCAT CTGGAGAGAG CCTCCCGGGT TCCCCAACTC ACAGCTTGTC CCCGAGGTCT 
CCAACACCCA GTTATCGTTC TACTCCCGAT TTTCCGTCAG GTACAAATTC CTCCCAGAGC 
AGCTCCCCAA GTTCAAGTGC CCCCAATTCT CCAGCAGGTT CAGGGCACAT CCGGCCCAGC 
ACCCTCCATG GCCTGGCTCC CAAACTCAGC GGGCAGCGAT ACCGCTCTGG AAGACGGAAG 
TCGGCTGGCA GCATCCCTCT CTCCCCGCTG GCCAGGACAC CCTCTCCCAC TCCACAGCCT 
ACCTCTCCTC AGCGTTCACC ATCCCCACTG TTGGGACACT CACTGGGCAA TGCCAAGATC 
ACTCAGGCCT TTCCTAGCAA GATGCACTCT CCCCCCACAA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CAGAGCCGCC CCGCTCCCCA CTGCTGAAAC GGGTGCAGTC GGAGGAAAAG 
TTGTCACCCT CCTATGGCAG TGACAAGAAG CTTCTGTGCT CCCGCAAGCA TAGCCTAGAG 
GTGACACAAG AGGAGGTACA GAGGGAGCAG TGTCAGCGGG AAGTGACACT GCAGAGCCTG 
GAAGAGAATG TGTGTGACGC TCCTTCCCTC AGTCGGGCCA GGCCAGTGGA GCAAGGCTGT 
CTGAAACGCC CCGTGTCCCG GAAGGTGGGC AGGCAAGAGT CTGTGGATGA CCTGGACCGG 
GACAAGCTGA AAGCCAAGGT TGTCGTAAAG AAACCAGAAG AGAAACATGA ATCGCACCAG 
AAACCTCACA GCCTTGGTGG TGATTCGGAA AGCTATGCTC TCTTCAGGCT AGAGGAGAGA 
GAGAAAAAAG TGTACTCCAA GGGGTTGGAA AGGTCAGGCC ATTTTGAAAA CACATCAGCA 
GAGTTGCCTT CTGTGGGCAG CCTGCTGAAG GACACTCTTC ACAAGCAGGC CAGCGTGAGG 
GCCAGCGAGG GGGTGACCTC AGACGGGGCA GCTTGCAGCC TGACACCAGG GGAGCACAGC 
CAGTCTCTAG GTGACTTTAA GCGGGCCTCA GCTTCTGGCA TTCTCCATGA TAGTGTGTGC 
CCCATCTCTG ATAGGCCTGC TCCTGGAAAG GTTGAATACT CGGAGAAGGC CTCTCAGGCC 
AAAGAGCTCC TTCGAAGTGA AAAACTAGAC AGCAAGCTGG CCAATATTGA TTACCTCAGA 
AAGAAAATGT CATTGGATGA CAAAGATGAC AGCCACTGTG CCATCCTGAA ACCCAAGATA 
ACATCTAGCG CCCATGAATG TCTGCCAGGG AACCCCATAC GGCCCATGGC AGGGCAACAA 
GAGACCCCGC CAGCCTCTGA AAACCGGGCA TTCATCAACA GTACCCACAC ACCTCAGATG 
AGTGCAGTTT CCTTTGTTCC TCTCAAAGCC TTAGCTGGCC GGGTAGAGAA CGGAGGGGAG 
AAAGCAGGCT TAGCTGCTCC CGAGTCCCCT GTCAGGAAGA GCCCCTCCGA GTATAAGCTA 
GAGGGCAGGT CAGTTTCATG TCTCAAGCCG ATCGAGGGCA CACTGGACAT TGCTCTCCTG 
TCTGGACCTC ACGCCTCCAA AACAGAGTTG CTTTCCCCAG AGCCTGCACA GAGTCCCAGC 
CCAGGCATCA ACGTGGGACC ATGTGTGCCA CTAGCTCTTC CTGGGAGCAG TGGGAAAAAG 
GGAGACTCCA CCAGCCTGAG AGAGCCTTCC TCAGCCAACT TAAAAGTAAA TAAATCTTAT 
CTGCTGGAGC CTCGGTTCCT ACCCCCGAGC CGGGCTCTCC AGGACTCTCT CGCAGCCTCT 
GGGCCAGAAC CGAAGTCAAA GCCGGAAAGG AAGCTCATTC ATCCTTCTGC CAGGAGCCCA 
GCAACTGTCA CAGAGAGCAA TCTTCAGCAG AAAGAGGGTG GTCCCGCCAC ACACCAAGAC 
CGCTCCACTG ACACCAGGAA CCTCCCTGGC CCAGGGCAGA CCCTACACAA TGTGGACCTA 
CCCAGGCTGT GTACACGTGC CCCACTCCCA CCGGAAGGGA CGCCCGCAAA GGAGAAGCCA 
TGTCTGAAGG AACCCTCTGC CAAGGTGAAA AGCGAGTGGT CTGCCGTGAG GGATGACGGA 
CACAGAGATC CCTGTGCGAA GCTGTGCCCG GCAGAGACTG GTAAAGCCAG CGACAGTTCC 
AAACCCCTGC CTTCCGGGGG GAGGACCCAA CCCGATTTCT ACAAGCAGAC CCAGACTTCG 
GAGAAAGCAT GGGCGCATGC AAAAACAAAC CACAAAGATA GCCAAGATGA GGTGAAGTCC 
CTGGCCAGGG AGGACTCAGC TTCACTTTTA TATGAAAAGG AGATAGGCCG GGCACGAAAA 
GGTCCTGAAC CCAAACCGGA AGTTCCTGCT ACCCGGTGCC CTCCTCAGCC ACCAGGAATT 
GAGGGTGAGA AGCGAGAAAA GCTCTCCGCT GCCCCCTCTT TGCAGAAACA GGCTCCCAAA 
GAGCCAGACA GGAAGGAACA GACTTCGCAA AGGCCTGGAG GTAGTGGCCC TCAACAACCC 
CCACCCACCA AAGAGCTGTC TAACTCAGCA TCCTGGCAGC ACGGCAGTTC TCCGAGTCAC 
ACTTTAAAGA AGGAGCCCGG GACCAAAGCT GCCGCTGCAG AACCAAGCAC CAGCCTTCAT 
GACACTCCCC GATCTGCTAC AGCCACCACC ACTGCCATTG CCACCACCAC CACTACCACC 
AGTGCCGGGC ACAGTGACTG CAGTAGCCAT AAGGCCCGGC CTGGCCCTGA CCCCAGCCCT 
TCAAAGTCTA AGCACCAAGA CAGGTCCCTC TCCTCACAGA AGCTGAGTGC TGGCTCTGCA 
AAAGGCAAAG AGCCTGTCAC TCAACCCCTG GGTGGTTCCA TCAGAGAAGG CAAGGGTGGC 
AGCAAGGGTC CAGTGGACAC ATTTTCTGCT GTCCTGACCA CCCAGGGCAA AGCAAGTGAT 
GTGCTTGTGC AGGGAGAAGG TCGGGTCTCA ATCATTGTCC ACACTGAAGA GTGTCCTCTC 
GATGCCAAAC TGAAAAACAC CAATGGAGGG TGTCCCCCAG AGATGCAGGC GAAGCATCCA 
CCCAGACAAG GACATCTCAG TGAAGCAGCA GACCAGAAGC CACTCATTGC TGGTGAGAAG 
CAAAGCCCGT CTCCAAAGCA TCCCAAACCA TCCACTGTGA AAGATTACCC CAGTCTGTGC 
AGACAGACAG ACAGAAGCCC AAGCCATCAG GCTACCACTG GGGACAGGAA GGCAGAAGGA 
AAGAAATGCA CAGACGCACT TTATGTCGCA GCCCCAGAGG GCTACAAGCC AGAGGCCAGC 
CCTTCTCTCC ACCACGGCGA GACCGGACTC AGAGGCTCAG AGAGGCCACC CATGGGCATG 
GGGAAGGGCT TCTCTGAGCC CAAGGGGAAA GGGCCAGGTC CCCAGAAGTC ACTGGCTGAA 
ACAGGCAAGC CCAGCGGTAT GAAAAGGTCA CCCTCTGCCA CCGTGCAGAG CTCTCTCCGC 
TCAGCTGCCC CCCCAGAAAA GTCTCTGAGT TACTCAGCCA GCTTTCCCGA GGCCCAGCCT 
GGAGTGCGAG AGGTCCCTGC AGCCAACAGC AGCCCCTCAT CTGCCAAGGC TACAGGGGGG 
ACCTCAGAGT TCCCAGCCCC CAGCAGCAGG GACCACAGGA AGCTTCAGTC TGGAGGAGAC 
GGCCGAAGCC AAATGATAAA GAGTGACTCT CTGCCCTCCT TCCGCCTCTC CACCTCTGCT 
CTGGAGTCAC ATTTCCAGGA TCCACAGGTG CCCATCGCAT CAGGCCACCG AGGCAGGGCA 
CTGTCAGTAA CTGCTGCCAC AGGAGAACCC AAAGGGAGAG AGCTCGCCCA GCCTCCCCCA 
GTCAGGAAAC AGAATGCGTG CAGAGAGGCG ACCAGAGCAC CCCCAGCCCC AAGCACAGAT 
CGCTCCCTCC CTCTTTCCTC AGAGAAAGAC TTCGTGGTTC GGCAGAGAAG GGGCAAGGAG 
ACCTTAAGGA GCAGTCCTCA CAAAAAGGCC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171021.1
CDS	908..8683
Translation	MGEKVSEAPEPVPRGCSGHGARTLVSSAAAVSSEGASSAESSSGSETLSEEGEPSRFSCRSQPPRPPGGALGTRLPAAWAPARVALERGVPTLPLPHPGGAVLPVPQVSSASQEEQDEELDHILSPPPMPFRKCSNPDVACGLGKSLKYKRQLSEDGKQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLEGQPPKSHFNSARHRQRLVDPAASKKELSLPRRGSLVDSQKWNCLVKRCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_017315532.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315532.1

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCCGA GGCGCCTGAG CCCGTGCCCC GGGGCTGCAG CGGACACGGC 
GCCCGGACCC TAGTCTCTTC GGCGGCAGCC GTGTCCTCGG AGGGCGCTTC CTCAGCGGAG 
TCATCCTCTG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CCAGCCGCTT CTCCTGCAGG 
TCGCAGCCGC CGCGGCCGCC GGGCGGCGCC CTGGGAACCC GGCTACCCGC CGCGTGGGCT 
CCCGCGCGCG TGGCTCTGGA GCGTGGAGTC CCTACCCTGC CGCTGCCGCA CCCGGGAGGA 
GCGGTGCTGC CGGTGCCCCA GGTCAGCAGC GCATCCCAAG AGGAGCAGGA TGAAGAGCTT 
GACCACATAC TGTCTCCGCC ACCCATGCCG TTTCGGAAAT GCAGCAACCC AGATGTGGCC 
TGCGGCCTCG GAAAATCACT GAAGTACAAG AGACAGCTTA GTGAGGATGG GAAGCAGCTG 
CGGCGGGGGA GCCTGGGAGG AGCCCTCACA GGGAGGTACC TCCTTCCAAA CCCGGTAGCA 
GGACAGGCCT GGCCTGCTTC GGCGGAGACG TCCAACCTCG TGCGCATGCG CAGCCAGGCC 
CTTGGCCAAT CGGCTCCCTC GCTCACAGCC AGCTTGGAAG GGCAACCTCC GAAGAGCCAT 
TTCAACTCAG CTCGACATCG GCAGAGACTA GTGGACCCAG CTGCTTCAAA AAAGGAGCTG 
AGCCTCCCCC GAAGAGGAAG TCTGGTAGAT TCCCAGAAGT GGAATTGCTT GGTCAAACGT 
TGCCGAACAA GCAACCGGAA GAGTTTGATA GGCAATGGCC AGTCTCCAGC ACTGCCTCGA 
CCACACTCAC CTCTCTCTGC TCATGCAGGA AATAGCCCTC AAGACAGTCC AAGGAATTTC 
TCCCCCAGTG CCTCAGCCCA TTTCTCATTT GCGAGGAGAA ATGACAGAAC GGATGGGCGC 
CGCTGGTCTC TGGCTTCTCT CCCTTCCTCA GGCTATGGGA CAAATACCCC AAGCTCCACC 
GTCTCCTCAT CCTGTTCTTC CCAGGAGAAG TTGCACCAGC TACCATACCA GCCAACCCCA 
GATGAATTAC ACTTCTTATC CAAACACTTC TGCACAACAG AAAGTATCGC CACTGAGAAC 
CGGTGCAGAA ACACACCCAT GCGTCCACGT TCCCGGAGTC TCAGCCCTGG ACGGTCCCCC 
GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG 
GCCACAGCTC AGATGGAAGA GCGTCTGAAG GAGATCATCA CCAGCTACTC TCCAGACCAT 
GTTCTCCCCT TGGCAGATGG GGTACTTAGT TTCACTCACC ATCAGATCAT TGAGCTGGCT 
CGGGACTGTT TGGATAAATC TCACCAGGGT CTCATCACGT CGAGATACTT TTTTGAGTTG 
CAGCACAAAC TGGACAAGTT GCTCCAGGAG GCTCACGATC GCTCTGAAAG TGGAGAACTG 
GCATTTATCA AGCAACTAGT CCGAAAGATC CTAATTGTCA TTGCCCGCCC CGCTCGGTTA 
TTGGAGTGTT TGGAATTTGA TCCTGAAGAA TTTTATTACC TATTGGAAGC TGCAGAAGGC 
CATGCCAAAG AAGGCCAAGG AATCAAAACT GACATCCCTA GGTATATCAT CAGCCAGCTG 
GGACTCAATA AGGACCCTTT AGAAGAAATG GCTCAGTTGG GGAATTACGA CAGTAGGACA 
GCAGAGACAC CAGAGATGGA TGAGTCAGTG AGTAGCTCAA ATACTTCCCT GAGACTTCGA 
AGGAAACCCC GAGAGAGTGA TTTTGAAACA ATTAAATTGA TCAGCAATGG AGCCTACGGG 
GCAGTCTACT TTGTTCGGCA CAAAGAGTCC CGCCAGAGGT TTGCCATGAA GAAGATCAAC 
AAGCAGAACC TCATCCTTCG GAACCAGATC CAGCAGGCCT TCGTGGAGCG AGACATCCTG 
ACTTTCGCAG AGAACCCCTT TGTGGTCAGC ATGTATTGCT CCTTTGAAAC GAGGCGTCAC 
TTATGCATGG TCATGGAGTA TGTAGAAGGG GGAGACTGTG CGACCCTAAT GAAAAACATG 
GGACCTCTCC CAGTTGATAT GGCCAGAATG TATTTCGCCG AGACCGTCTT GGCCTTGGAG 
TACCTGCATA ATTACGGAAT CGTACACAGG GACTTGAAGC CAGACAACCT GTTGGTCACC 
TCCATGGGGC ACATAAAACT GACTGACTTC GGCTTGTCTA AGGTGGGATT AATGAGCATG 
ACCACCAATC TCTATGAAGG CCACATAGAG AAGGACGCTC GAGAGTTCTT AGATAAACAG 
GTCTGTGGTA CACCTGAGTA CATTGCCCCC GAGGTGATTC TGAGACAGGG CTACGGGAAA 
CCCGTGGACT GGTGGGCCAT GGGCATTATC CTCTATGAAT TTCTGGTCGG ATGTGTGCCT 
TTCTTTGGGG ACACTCCAGA AGAGCTATTT GGACAAGTCA TCAGTGATGA AATCAACTGG 
CCTGAAAAGG ACGAGGCCCC TCCTCCAGAC GCTCAGGAGC TGATTACCTT GCTCCTCAGG 
CAGAATCCGC TGGAGAGGCT GGGAACAGGT GGAGCCTATG AAGTGAAGCA GCATCGTTTC 
TTCCGCTCCT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CGGAATTTAT TCCCCAACTG 
GAATCGGAGG ATGACACAAG TTATTTTGAT ACTCGGTCAG AGAAGTATCA TCACATGGAG 
ACGGAGGAGG AGGACGATAC AAACGATGAG GACTTCACCG TGGAGATAAG GCAGTTTTCT 
TCCTGTTCAC ACAGGTTTTC AAAAGTTTTC AGCAGTATAG ATCGCATAAC TCAAAATTCA 
GGAGAAGACA AAGATGACTC TGAGGACAAG ACCAAAAGCA CAACGTTGCC ATCCACAGAG 
ACACTCAGCT GGAGTTCCGA ATACTCTGAA ATGCAACAGT TATCGACCTC CAACTCTTCA 
GATACTGAAA GCAACAGGTG CAAACTCAGC TCTGGCTTGC TCCCCAAGCT GGCTATTTCG 
ACAGATGGGG AACAAGATGA GGCTGTCCCT TGCTCTGGAG ACCCCAGAGA GGAGCCAGAG 
AAACCTGTCC CTCCCTCTGA GGAGTGTACT CAGGAGGAGC CCGAGGTCAC CACCCCAGCC 
AGCACCATCA GCAGTTCCAC ACTGTCAGAT ATGTTCGCTG TATCTCCATT GGGAAGCCCA 
ATGTCCCCAC ACTCCCTGTC TTCAGACCCT TCTTCTTCAC GGGATTCCTC TCCCAGCCGA 
GACTCTTCTG CAGCATCTGC CAGTCCGCAT CAGCCCATTG TCATCCACAG CTCAGGCAAG 
AACTATGGGT TCACCATCCG TGCTATCCGC GTGTACGTGG GGGACAGTGA CATCTACACA 
GTGCACCATA TCGTCTGGAA CGTAGAAGAA GGAAGTCCCG CATACCAGGC AGGACTGAAG 
GCCGGGGATC TGATCACACA CATCAACGGA GAGCCGGTGC ACGGCCTCGT CCACACGGAA 
GTTATCGAGC TCCTGCTGAA GAGTGGGAAT AAGGTGTCTA TCACCACTAC TCCATTTGAA 
AACACATCAA TCAAAACGGG ACCAGCCAGG AGAAACAGTT ACAAGGGCCG GATGGTGAGA 
CGAAGCAAGA AGTCCAAGAA GAAGGAGAGT CTAGAAAGGA GGAGATCTCT CTTCAAGAAG 
CTGGCCAAGC AGCCTTCTCC TTTGCTCCAC ACCAGCCGAA GTTTCTCCTG CTTAAACCGG 
TCCCTGTCAT CTGGAGAGAG CCTCCCGGGT TCCCCAACTC ACAGCTTGTC CCCGAGGTCT 
CCAACACCCA GTTATCGTTC TACTCCCGAT TTTCCGTCAG GTACAAATTC CTCCCAGAGC 
AGCTCCCCAA GTTCAAGTGC CCCCAATTCT CCAGCAGGTT CAGGGCACAT CCGGCCCAGC 
ACCCTCCATG GCCTGGCTCC CAAACTCAGC GGGCAGCGAT ACCGCTCTGG AAGACGGAAG 
TCGGCTGGCA GCATCCCTCT CTCCCCGCTG GCCAGGACAC CCTCTCCCAC TCCACAGCCT 
ACCTCTCCTC AGCGTTCACC ATCCCCACTG TTGGGACACT CACTGGGCAA TGCCAAGATC 
ACTCAGGCCT TTCCTAGCAA GATGCACTCT CCCCCCACAA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CAGAGCCGCC CCGCTCCCCA CTGCTGAAAC GGGTGCAGTC GGAGGAAAAG 
TTGTCACCCT CCTATGGCAG TGACAAGAAG CTTCTGTGCT CCCGCAAGCA TAGCCTAGAG 
GTGACACAAG AGGAGGTACA GAGGGAGCAG TGTCAGCGGG AAGTGACACT GCAGAGCCTG 
GAAGAGAATG TGTGTGACGC TCCTTCCCTC AGTCGGGCCA GGCCAGTGGA GCAAGGCTGT 
CTGAAACGCC CCGTGTCCCG GAAGGTGGGC AGGCAAGAGT CTGTGGATGA CCTGGACCGG 
GACAAGCTGA AAGCCAAGGT TGTCGTAAAG AAACCAGAAG AGAAACATGA ATCGCACCAG 
AAACCTCACA GCCTTGGTGG TGATTCGGAA AGCTATGCTC TCTTCAGGCT AGAGGAGAGA 
GAGAAAAAAG TGTACTCCAA GGGGTTGGAA AGGTCAGGCC ATTTTGAAAA CACATCAGCA 
GAGTTGCCTT CTGTGGGCAG CCTGCTGAAG GACACTCTTC ACAAGCAGGC CAGCGTGAGG 
GCCAGCGAGG GGGTGACCTC AGACGGGGCA GCTTGCAGCC TGACACCAGG GGAGCACAGC 
CAGTCTCTAG GTGACTTTAA GCGGGCCTCA GCTTCTGGCA TTCTCCATGA TAGTGTGTGC 
CCCATCTCTG ATAGGCCTGC TCCTGGAAAG GTTGAATACT CGGAGAAGGC CTCTCAGGCC 
AAAGAGCTCC TTCGAAGTGA AAAACTAGAC AGCAAGCTGG CCAATATTGA TTACCTCAGA 
AAGAAAATGT CATTGGATGA CAAAGATGAC AGCCACTGTG CCATCCTGAA ACCCAAGATA 
ACATCTAGCG CCCATGAATG TCTGCCAGGG AACCCCATAC GGCCCATGGC AGGGCAACAA 
GAGACCCCGC CAGCCTCTGA AAACCGGGCA TTCATCAACA GTACCCACAC ACCTCAGATG 
AGTGCAGTTT CCTTTGTTCC TCTCAAAGCC TTAGCTGGCC GGGTAGAGAA CGGAGGGGAG 
AAAGCAGGCT TAGCTGCTCC CGAGTCCCCT GTCAGGAAGA GCCCCTCCGA GTATAAGCTA 
GAGGGCAGGT CAGTTTCATG TCTCAAGCCG ATCGAGGGCA CACTGGACAT TGCTCTCCTG 
TCTGGACCTC ACGCCTCCAA AACAGAGTTG CTTTCCCCAG AGCCTGCACA GAGTCCCAGC 
CCAGGCATCA ACGTGGGACC ATGTGTGCCA CTAGCTCTTC CTGGGAGCAG TGGGAAAAAG 
GGAGACTCCA CCAGCCTGAG AGAGCCTTCC TCAGCCAACT TAAAAGTAAA TAAATCTTAT 
CTGCTGGAGC CTCGGTTCCT ACCCCCGAGC CGGGCTCTCC AGGACTCTCT CGCAGCCTCT 
GGGCCAGAAC CGAAGTCAAA GCCGGAAAGG AAGCTCATTC ATCCTTCTGC CAGGAGCCCA 
GCAACTGTCA CAGAGAGCAA TCTTCAGCAG AAAGAGGGTG GTCCCGCCAC ACACCAAGAC 
CGCTCCACTG ACACCAGGAA CCTCCCTGGC CCAGGGCAGA CCCTACACAA TGTGGACCTA 
CCCAGGCTGT GTACACGTGC CCCACTCCCA CCGGAAGGGA CGCCCGCAAA GGAGAAGCCA 
TGTCTGAAGG AACCCTCTGC CAAGGTGAAA AGCGAGTGGT CTGCCGTGAG GGATGACGGA 
CACAGAGATC CCTGTGCGAA GCTGTGCCCG GCAGAGACTG GTAAAGCCAG CGACAGTTCC 
AAACCCCTGC CTTCCGGGGG GAGGACCCAA CCCGATTTCT ACAAGCAGAC CCAGACTTCG 
GAGAAAGCAT GGGCGCATGC AAAAACAAAC CACAAAGATA GCCAAGATGA GGTGAAGTCC 
CTGGCCAGGG AGGACTCAGC TTCACTTTTA TATGAAAAGG AGATAGGCCG GGCACGAAAA 
GGTCCTGAAC CCAAACCGGA AGTTCCTGCT ACCCGGTGCC CTCCTCAGCC ACCAGGAATT 
GAGGGTGAGA AGCGAGAAAA GCTCTCCGCT GCCCCCTCTT TGCAGAAACA GGCTCCCAAA 
GAGCCAGACA GGAAGGAACA GACTTCGCAA AGGCCTGGAG GTAGTGGCCC TCAACAACCC 
CCACCCACCA AAGAGCTGTC TAACTCAGCA TCCTGGCAGC ACGGCAGTTC TCCGAGTCAC 
ACTTTAAAGA AGGAGCCCGG GACCAAAGCT GCCGCTGCAG AACCAAGCAC CAGCCTTCAT 
GACACTCCCC GATCTGCTAC AGCCACCACC ACTGCCATTG CCACCACCAC CACTACCACC 
AGTGCCGGGC ACAGTGACTG CAGTAGCCAT AAGGCCCGGC CTGGCCCTGA CCCCAGCCCT 
TCAAAGTCTA AGCACCAAGA CAGGTCCCTC TCCTCACAGA AGCTGAGTGC TGGCTCTGCA 
AAAGGCAAAG AGCCTGTCAC TCAACCCCTG GGTGGTTCCA TCAGAGAAGG CAAGGGTGGC 
AGCAAGGGTC CAGTGGACAC ATTTTCTGCT GTCCTGACCA CCCAGGGCAA AGCAAGTGAT 
GTGCTTGTGC AGGGAGAAGG TCGGGTCTCA ATCATTGTCC ACACTGAAGA GTGTCCTCTC 
GATGCCAAAC TGAAAAACAC CAATGGAGGG TGTCCCCCAG AGATGCAGGC GAAGCATCCA 
CCCAGACAAG GACATCTCAG TGAAGCAGCA GACCAGAAGC CACTCATTGC TGGTGAGAAG 
CAAAGCCCGT CTCCAAAGCA TCCCAAACCA TCCACTGTGA AAGATTACCC CAGTCTGTGC 
AGACAGACAG ACAGAAGCCC AAGCCATCAG GCTACCACTG GGGACAGGAA GGCAGAAGGA 
AAGAAATGCA CAGACGCACT TTATGTCGCA GCCCCAGAGG GCTACAAGCC AGAGGCCAGC 
CCTTCTCTCC ACCACGGCGA GACCGGACTC AGAGGCTCAG AGAGGCCACC CATGGGCATG 
GGGAAGGGCT TCTCTGAGCC CAAGGGGAAA GGGCCAGGTC CCCAGAAGTC ACTGGCTGAA 
ACAGGCAAGC CCAGCGGTAT GAAAAGGTCA CCCTCTGCCA CCGTGCAGAG CTCTCTCCGC 
TCAGCTGCCC CCCCAGAAAA GTCTCTGAGT TACTCAGCCA GCTTTCCCGA GGCCCAGCCT 
GGAGTGCGAG AGGTCCCTGC AGCCAACAGC AGCCCCTCAT CTGCCAAGGC TACAGGGGGG 
ACCTCAGAGT TCCCAGCCCC CAGCAGCAGG GACCACAGGA AGCTTCAGTC TGGAGGAGAC 
GGCCGAAGCC AAATGATAAA GAGTGACTCT CTGCCCTCCT TCCGCCTCTC CACCTCTGCT 
CTGGAGTCAC ATTTCCAGGA TCCACAGGTG CCCATCGCAT CAGGCCACCG AGGCAGGGCA 
CTGTCAGTAA CTGCTGCCAC AGGAGAACCC AAAGGGAGAG AGCTCGCCCA GCCTCCCCCA 
GTCAGGAAAC AGAATGCGTG CAGAGAGGCG ACCAGAGCAC CCCCAGCCCC AAGCACAGAT 
CGCTCCCTCC CTCTTTCCTC AGAGAAAGAC TTCGTGGTTC GGCAGAGAAG GGGCAAGGAG 
ACCTTAAGGA GCAGTCCTCA CAAAAAGGCC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mast4 ( XM_017315530.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171019.1 Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X4, mRNA.

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CloneID	OMu78478
Clone ID Related Accession (Same CDS sequence)	XM_017315530.1
Accession Version	XM_017315530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AAGTTTCCGA GGCGCCTGAG CCCGTGCCCC GGGGCTGCAG CGGACACGGC 
GCCCGGACCC TAGTCTCTTC GGCGGCAGCC GTGTCCTCGG AGGGCGCTTC CTCAGCGGAG 
TCATCCTCTG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CCAGCCGCTT CTCCTGCAGG 
TCGCAGCCGC CGCGGCCGCC GGGCGGCGCC CTGGGAACCC GGCTACCCGC CGCGTGGGCT 
CCCGCGCGCG TGGCTCTGGA GCGTGGAGTC CCTACCCTGC CGCTGCCGCA CCCGGGAGGA 
GCGGTGCTGC CGGTGCCCCA GGTCAGCAGC GCATCCCAAG AGGAGCAGGA TGAAGAGCTT 
GACCACATAC TGTCTCCGCC ACCCATGCCG TTTCGGAAAT GCAGCAACCC AGATGTGGCC 
TGCGGCCTCG GAAAATCACT GAAGTACAAG AGACAGCTTA GTGAGGATGG GAAGCAGCTG 
CGGCGGGGGA GCCTGGGAGG AGCCCTCACA GGGAGGTACC TCCTTCCAAA CCCGGTAGCA 
GGACAGGCCT GGCCTGCTTC GGCGGAGACG TCCAACCTCG TGCGCATGCG CAGCCAGGCC 
CTTGGCCAAT CGGCTCCCTC GCTCACAGCC AGCTTGAAGG AGCTGAGCCT CCCCCGAAGA 
GGAAGTCTGG TAGATTCCCA GAAGTGGAAT TGCTTGGTCA AACGTTGCCG AACAAGCAAC 
CGGAAGAGTT TGATAGGCAA TGGCCAGTCT CCAGCACTGC CTCGACCACA CTCACCTCTC 
TCTGCTCATG CAGGAAATAG CCCTCAAGAC AGTCCAAGGA ATTTCTCCCC CAGTGCCTCA 
GCCCATTTCT CATTTGCGAG GAGAAATGAC AGAACGGATG GGCGCCGCTG GTCTCTGGCT 
TCTCTCCCTT CCTCAGGCTA TGGGACAAAT ACCCCAAGCT CCACCGTCTC CTCATCCTGT 
TCTTCCCAGG AGAAGTTGCA CCAGCTACCA TACCAGCCAA CCCCAGATGA ATTACACTTC 
TTATCCAAAC ACTTCTGCAC AACAGAAAGT ATCGCCACTG AGAACCGGTG CAGAAACACA 
CCCATGCGTC CACGTTCCCG GAGTCTCAGC CCTGGACGGT CCCCCGCCTG CTGTGACCAT 
GAAATAATTA TGATGAACCA TGTCTACAAA GAAAGGTTCC CAAAGGCCAC AGCTCAGATG 
GAAGAGCGTC TGAAGGAGAT CATCACCAGC TACTCTCCAG ACCATGTTCT CCCCTTGGCA 
GATGGGGTAC TTAGTTTCAC TCACCATCAG ATCATTGAGC TGGCTCGGGA CTGTTTGGAT 
AAATCTCACC AGGGTCTCAT CACGTCGAGA TACTTTTTTG AGTTGCAGCA CAAACTGGAC 
AAGTTGCTCC AGGAGGCTCA CGATCGCTCT GAAAGTGGAG AACTGGCATT TATCAAGCAA 
CTAGTCCGAA AGATCCTAAT TGTCATTGCC CGCCCCGCTC GGTTATTGGA GTGTTTGGAA 
TTTGATCCTG AAGAATTTTA TTACCTATTG GAAGCTGCAG AAGGCCATGC CAAAGAAGGC 
CAAGGAATCA AAACTGACAT CCCTAGGTAT ATCATCAGCC AGCTGGGACT CAATAAGGAC 
CCTTTAGAAG AAATGGCTCA GTTGGGGAAT TACGACAGTA GGACAGCAGA GACACCAGAG 
ATGGATGAGT CAGTGAGTAG CTCAAATACT TCCCTGAGAC TTCGAAGGAA ACCCCGAGAG 
AGTGATTTTG AAACAATTAA ATTGATCAGC AATGGAGCCT ACGGGGCAGT CTACTTTGTT 
CGGCACAAAG AGTCCCGCCA GAGGTTTGCC ATGAAGAAGA TCAACAAGCA GAACCTCATC 
CTTCGGAACC AGATCCAGCA GGCCTTCGTG GAGCGAGACA TCCTGACTTT CGCAGAGAAC 
CCCTTTGTGG TCAGCATGTA TTGCTCCTTT GAAACGAGGC GTCACTTATG CATGGTCATG 
GAGTATGTAG AAGGGGGAGA CTGTGCGACC CTAATGAAAA ACATGGGACC TCTCCCAGTT 
GATATGGCCA GAATGTATTT CGCCGAGACC GTCTTGGCCT TGGAGTACCT GCATAATTAC 
GGAATCGTAC ACAGGGACTT GAAGCCAGAC AACCTGTTGG TCACCTCCAT GGGGCACATA 
AAACTGACTG ACTTCGGCTT GTCTAAGGTG GGATTAATGA GCATGACCAC CAATCTCTAT 
GAAGGCCACA TAGAGAAGGA CGCTCGAGAG TTCTTAGATA AACAGGTCTG TGGTACACCT 
GAGTACATTG CCCCCGAGGT GATTCTGAGA CAGGGCTACG GGAAACCCGT GGACTGGTGG 
GCCATGGGCA TTATCCTCTA TGAATTTCTG GTCGGATGTG TGCCTTTCTT TGGGGACACT 
CCAGAAGAGC TATTTGGACA AGTCATCAGT GATGAAATCA ACTGGCCTGA AAAGGACGAG 
GCCCCTCCTC CAGACGCTCA GGAGCTGATT ACCTTGCTCC TCAGGCAGAA TCCGCTGGAG 
AGGCTGGGAA CAGGTGGAGC CTATGAAGTG AAGCAGCATC GTTTCTTCCG CTCCTTAGAC 
TGGAACAGTT TGCTGAGACA GAAGGCGGAA TTTATTCCCC AACTGGAATC GGAGGATGAC 
ACAAGTTATT TTGATACTCG GTCAGAGAAG TATCATCACA TGGAGACGGA GGAGGAGGAC 
GATACAAACG ATGAGGACTT CACCGTGGAG ATAAGGCAGT TTTCTTCCTG TTCACACAGG 
TTTTCAAAAG TTTTCAGCAG TATAGATCGC ATAACTCAAA ATTCAGGAGA AGACAAAGAT 
GACTCTGAGG ACAAGACCAA AAGCACAACG TTGCCATCCA CAGAGACACT CAGCTGGAGT 
TCCGAATACT CTGAAATGCA ACAGTTATCG ACCTCCAACT CTTCAGATAC TGAAAGCAAC 
AGGTGCAAAC TCAGCTCTGG CTTGCTCCCC AAGCTGGCTA TTTCGACAGA TGGGGAACAA 
GATGAGGCTG TCCCTTGCTC TGGAGACCCC AGAGAGGAGC CAGAGAAACC TGTCCCTCCC 
TCTGAGGAGT GTACTCAGGA GGAGCCCGAG GTCACCACCC CAGCCAGCAC CATCAGCAGT 
TCCACACTGT CAGTTGGCAG TTTTTCAGAG CACTTGGATC AGATAAATGG GCGAAGCGAG 
TGTGTGGACA GTACAGATAA TTCCTCAAAG CCATCCAGTG AACCCACTTC TCACGTGGCT 
CGACAGCGCT TAGAAAGCAC AGAGAAAAAG AAAATTTCTG GGAAAGTCAC AAAGTCCCTC 
TCGGCCAGTG CTCTGTCCCT CATGATCCCA GGAGATATGT TCGCTGTATC TCCATTGGGA 
AGCCCAATGT CCCCACACTC CCTGTCTTCA GACCCTTCTT CTTCACGGGA TTCCTCTCCC 
AGCCGAGACT CTTCTGCAGC ATCTGCCAGT CCGCATCAGC CCATTGTCAT CCACAGCTCA 
GGCAAGAACT ATGGGTTCAC CATCCGTGCT ATCCGCGTGT ACGTGGGGGA CAGTGACATC 
TACACAGTGC ACCATATCGT CTGGAACGTA GAAGAAGGAA GTCCCGCATA CCAGGCAGGA 
CTGAAGGCCG GGGATCTGAT CACACACATC AACGGAGAGC CGGTGCACGG CCTCGTCCAC 
ACGGAAGTTA TCGAGCTCCT GCTGAAGAGT GGGAATAAGG TGTCTATCAC CACTACTCCA 
TTTGAAAACA CATCAATCAA AACGGGACCA GCCAGGAGAA ACAGTTACAA GGGCCGGATG 
GTGAGACGAA GCAAGAAGTC CAAGAAGAAG GAGAGTCTAG AAAGGAGGAG ATCTCTCTTC 
AAGAAGCTGG CCAAGCAGCC TTCTCCTTTG CTCCACACCA GCCGAAGTTT CTCCTGCTTA 
AACCGGTCCC TGTCATCTGG AGAGAGCCTC CCGGGTTCCC CAACTCACAG CTTGTCCCCG 
AGGTCTCCAA CACCCAGTTA TCGTTCTACT CCCGATTTTC CGTCAGGTAC AAATTCCTCC 
CAGAGCAGCT CCCCAAGTTC AAGTGCCCCC AATTCTCCAG CAGGTTCAGG GCACATCCGG 
CCCAGCACCC TCCATGGCCT GGCTCCCAAA CTCAGCGGGC AGCGATACCG CTCTGGAAGA 
CGGAAGTCGG CTGGCAGCAT CCCTCTCTCC CCGCTGGCCA GGACACCCTC TCCCACTCCA 
CAGCCTACCT CTCCTCAGCG TTCACCATCC CCACTGTTGG GACACTCACT GGGCAATGCC 
AAGATCACTC AGGCCTTTCC TAGCAAGATG CACTCTCCCC CCACAATCGT CAGACACATC 
GTGAGGCCCA AGAGTGCAGA GCCGCCCCGC TCCCCACTGC TGAAACGGGT GCAGTCGGAG 
GAAAAGTTGT CACCCTCCTA TGGCAGTGAC AAGAAGCTTC TGTGCTCCCG CAAGCATAGC 
CTAGAGGTGA CACAAGAGGA GGTACAGAGG GAGCAGTGTC AGCGGGAAGT GACACTGCAG 
AGCCTGGAAG AGAATGTGTG TGACGCTCCT TCCCTCAGTC GGGCCAGGCC AGTGGAGCAA 
GGCTGTCTGA AACGCCCCGT GTCCCGGAAG GTGGGCAGGC AAGAGTCTGT GGATGACCTG 
GACCGGGACA AGCTGAAAGC CAAGGTTGTC GTAAAGAAAC CAGAAGAGAA ACATGAATCG 
CACCAGAAAC CTCACAGCCT TGGTGGTGAT TCGGAAAGCT ATGCTCTCTT CAGGCTAGAG 
GAGAGAGAGA AAAAAGTGTA CTCCAAGGGG TTGGAAAGGT CAGGCCATTT TGAAAACACA 
TCAGCAGAGT TGCCTTCTGT GGGCAGCCTG CTGAAGGACA CTCTTCACAA GCAGGCCAGC 
GTGAGGGCCA GCGAGGGGGT GACCTCAGAC GGGGCAGCTT GCAGCCTGAC ACCAGGGGAG 
CACAGCCAGT CTCTAGGTGA CTTTAAGCGG GCCTCAGCTT CTGGCATTCT CCATGATAGT 
GTGTGCCCCA TCTCTGATAG GCCTGCTCCT GGAAAGGTTG AATACTCGGA GAAGGCCTCT 
CAGGCCAAAG AGCTCCTTCG AAGTGAAAAA CTAGACAGCA AGCTGGCCAA TATTGATTAC 
CTCAGAAAGA AAATGTCATT GGATGACAAA GATGACAGCC ACTGTGCCAT CCTGAAACCC 
AAGATAACAT CTAGCGCCCA TGAATGTCTG CCAGGGAACC CCATACGGCC CATGGCAGGG 
CAACAAGAGA CCCCGCCAGC CTCTGAAAAC CGGGCATTCA TCAACAGTAC CCACACACCT 
CAGATGAGTG CAGTTTCCTT TGTTCCTCTC AAAGCCTTAG CTGGCCGGGT AGAGAACGGA 
GGGGAGAAAG CAGGCTTAGC TGCTCCCGAG TCCCCTGTCA GGAAGAGCCC CTCCGAGTAT 
AAGCTAGAGG GCAGGTCAGT TTCATGTCTC AAGCCGATCG AGGGCACACT GGACATTGCT 
CTCCTGTCTG GACCTCACGC CTCCAAAACA GAGTTGCTTT CCCCAGAGCC TGCACAGAGT 
CCCAGCCCAG GCATCAACGT GGGACCATGT GTGCCACTAG CTCTTCCTGG GAGCAGTGGG 
AAAAAGGGAG ACTCCACCAG CCTGAGAGAG CCTTCCTCAG CCAACTTAAA AGTAAATAAA 
TCTTATCTGC TGGAGCCTCG GTTCCTACCC CCGAGCCGGG CTCTCCAGGA CTCTCTCGCA 
GCCTCTGGGC CAGAACCGAA GTCAAAGCCG GAAAGGAAGC TCATTCATCC TTCTGCCAGG 
AGCCCAGCAA CTGTCACAGA GAGCAATCTT CAGCAGAAAG AGGGTGGTCC CGCCACACAC 
CAAGACCGCT CCACTGACAC CAGGAACCTC CCTGGCCCAG GGCAGACCCT ACACAATGTG 
GACCTACCCA GGCTGTGTAC ACGTGCCCCA CTCCCACCGG AAGGGACGCC CGCAAAGGAG 
AAGCCATGTC TGAAGGAACC CTCTGCCAAG GTGAAAAGCG AGTGGTCTGC CGTGAGGGAT 
GACGGACACA GAGATCCCTG TGCGAAGCTG TGCCCGGCAG AGACTGGTAA AGCCAGCGAC 
AGTTCCAAAC CCCTGCCTTC CGGGGGGAGG ACCCAACCCG ATTTCTACAA GCAGACCCAG 
ACTTCGGAGA AAGCATGGGC GCATGCAAAA ACAAACCACA AAGATAGCCA AGATGAGGTG 
AAGTCCCTGG CCAGGGAGGA CTCAGCTTCA CTTTTATATG AAAAGGAGAT AGGCCGGGCA 
CGAAAAGGTC CTGAACCCAA ACCGGAAGTT CCTGCTACCC GGTGCCCTCC TCAGCCACCA 
GGAATTGAGG GTGAGAAGCG AGAAAAGCTC TCCGCTGCCC CCTCTTTGCA GAAACAGGCT 
CCCAAAGAGC CAGACAGGAA GGAACAGACT TCGCAAAGGC CTGGAGGTAG TGGCCCTCAA 
CAACCCCCAC CCACCAAAGA GCTGTCTAAC TCAGCATCCT GGCAGCACGG CAGTTCTCCG 
AGTCACACTT TAAAGAAGGA GCCCGGGACC AAAGCTGCCG CTGCAGAACC AAGCACCAGC 
CTTCATGACA CTCCCCGATC TGCTACAGCC ACCACCACTG CCATTGCCAC CACCACCACT 
ACCACCAGTG CCGGGCACAG TGACTGCAGT AGCCATAAGG CCCGGCCTGG CCCTGACCCC 
AGCCCTTCAA AGTCTAAGCA CCAAGACAGG TCCCTCTCCT CACAGAAGCT GAGTGCTGGC 
TCTGCAAAAG GCAAAGAGCC TGTCACTCAA CCCCTGGGTG GTTCCATCAG AGAAGGCAAG 
GGTGGCAGCA AGGGTCCAGT GGACACATTT TCTGCTGTCC TGACCACCCA GGGCAAAGCA 
AGTGATGTGC TTGTGCAGGG AGAAGGTCGG GTCTCAATCA TTGTCCACAC TGAAGAGTGT 
CCTCTCGATG CCAAACTGAA AAACACCAAT GGAGGGTGTC CCCCAGAGAT GCAGGCGAAG 
CATCCACCCA GACAAGGACA TCTCAGTGAA GCAGCAGACC AGAAGCCACT CATTGCTGGT 
GAGAAGCAAA GCCCGTCTCC AAAGCATCCC AAACCATCCA CTGTGAAAGA TTACCCCAGT 
CTGTGCAGAC AGACAGACAG AAGCCCAAGC CATCAGGCTA CCACTGGGGA CAGGAAGGCA 
GAAGGAAAGA AATGCACAGA CGCACTTTAT GTCGCAGCCC CAGAGGGCTA CAAGCCAGAG 
GCCAGCCCTT CTCTCCACCA CGGCGAGACC GGACTCAGAG GCTCAGAGAG GCCACCCATG 
GGCATGGGGA AGGGCTTCTC TGAGCCCAAG GGGAAAGGGC CAGGTCCCCA GAAGTCACTG 
GCTGAAACAG GCAAGCCCAG CGGTATGAAA AGGTCACCCT CTGCCACCGT GCAGAGCTCT 
CTCCGCTCAG CTGCCCCCCC AGAAAAGTCT CTGAGTTACT CAGCCAGCTT TCCCGAGGCC 
CAGCCTGGAG TGCGAGAGGT CCCTGCAGCC AACAGCAGCC CCTCATCTGC CAAGGCTACA 
GGGGGGACCT CAGAGTTCCC AGCCCCCAGC AGCAGGGACC ACAGGAAGCT TCAGTCTGGA 
GGAGACGGCC GAAGCCAAAT GATAAAGAGT GACTCTCTGC CCTCCTTCCG CCTCTCCACC 
TCTGCTCTGG AGTCACATTT CCAGGATCCA CAGGTGCCCA TCGCATCAGG CCACCGAGGC 
AGGGCACTGT CAGTAACTGC TGCCACAGGA GAACCCAAAG GGAGAGAGCT CGCCCAGCCT 
CCCCCAGTCA GGAAACAGAA TGCGTGCAGA GAGGCGACCA GAGCACCCCC AGCCCCAAGC 
ACAGATCGCT CCCTCCCTCT TTCCTCAGAG AAAGACTTCG TGGTTCGGCA GAGAAGGGGC 
AAGGAGACCT TAAGGAGCAG TCCTCACAAA AAGGCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171019.1
CDS	908..8809
Translation	MGEKVSEAPEPVPRGCSGHGARTLVSSAAAVSSEGASSAESSSGSETLSEEGEPSRFSCRSQPPRPPGGALGTRLPAAWAPARVALERGVPTLPLPHPGGAVLPVPQVSSASQEEQDEELDHILSPPPMPFRKCSNPDVACGLGKSLKYKRQLSEDGKQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLKELSLPRRGSLVDSQKWNCLVKRCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFFELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAQLGNYDSRTAETPEMDESVSSSNTSLRLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFTVEIRQFSSCSHRFSKVFSSIDRITQNSGEDKDDSEDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRCKLSSGLLPKLAISTDGEQDEAVPCSGDPREEPEKPVPPSEECTQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPTSHVARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKGRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLSGQRYRSGRRKSAGSIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNAKITQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKLLCSRKHSLEVTQEEVQREQCQREVTLQSLEENVCDAPSLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKPEEKHESHQKPHSLGGDSESYALFRLEEREKKVYSKGLERSGHFENTSAELPSVGSLLKDTLHKQASVRASEGVTSDGAACSLTPGEHSQSLGDFKRASASGILHDSVCPISDRPAPGKVEYSEKASQAKELLRSEKLDSKLANIDYLRKKMSLDDKDDSHCAILKPKITSSAHECLPGNPIRPMAGQQETPPASENRAFINSTHTPQMSAVSFVPLKALAGRVENGGEKAGLAAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPHASKTELLSPEPAQSPSPGINVGPCVPLALPGSSGKKGDSTSLREPSSANLKVNKSYLLEPRFLPPSRALQDSLAASGPEPKSKPERKLIHPSARSPATVTESNLQQKEGGPATHQDRSTDTRNLPGPGQTLHNVDLPRLCTRAPLPPEGTPAKEKPCLKEPSAKVKSEWSAVRDDGHRDPCAKLCPAETGKASDSSKPLPSGGRTQPDFYKQTQTSEKAWAHAKTNHKDSQDEVKSLAREDSASLLYEKEIGRARKGPEPKPEVPATRCPPQPPGIEGEKREKLSAAPSLQKQAPKEPDRKEQTSQRPGGSGPQQPPPTKELSNSASWQHGSSPSHTLKKEPGTKAAAAEPSTSLHDTPRSATATTTAIATTTTTTSAGHSDCSSHKARPGPDPSPSKSKHQDRSLSSQKLSAGSAKGKEPVTQPLGGSIREGKGGSKGPVDTFSAVLTTQGKASDVLVQGEGRVSIIVHTEECPLDAKLKNTNGGCPPEMQAKHPPRQGHLSEAADQKPLIAGEKQSPSPKHPKPSTVKDYPSLCRQTDRSPSHQATTGDRKAEGKKCTDALYVAAPEGYKPEASPSLHHGETGLRGSERPPMGMGKGFSEPKGKGPGPQKSLAETGKPSGMKRSPSATVQSSLRSAAPPEKSLSYSASFPEAQPGVREVPAANSSPSSAKATGGTSEFPAPSSRDHRKLQSGGDGRSQMIKSDSLPSFRLSTSALESHFQDPQVPIASGHRGRALSVTAATGEPKGRELAQPPPVRKQNACREATRAPPAPSTDRSLPLSSEKDFVVRQRRGKETLRSSPHKKAS

Target ORF information:

RefSeq Version	XM_017315530.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase family member 4 (Mast4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315530.1

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCCGA GGCGCCTGAG CCCGTGCCCC GGGGCTGCAG CGGACACGGC 
GCCCGGACCC TAGTCTCTTC GGCGGCAGCC GTGTCCTCGG AGGGCGCTTC CTCAGCGGAG 
TCATCCTCTG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CCAGCCGCTT CTCCTGCAGG 
TCGCAGCCGC CGCGGCCGCC GGGCGGCGCC CTGGGAACCC GGCTACCCGC CGCGTGGGCT 
CCCGCGCGCG TGGCTCTGGA GCGTGGAGTC CCTACCCTGC CGCTGCCGCA CCCGGGAGGA 
GCGGTGCTGC CGGTGCCCCA GGTCAGCAGC GCATCCCAAG AGGAGCAGGA TGAAGAGCTT 
GACCACATAC TGTCTCCGCC ACCCATGCCG TTTCGGAAAT GCAGCAACCC AGATGTGGCC 
TGCGGCCTCG GAAAATCACT GAAGTACAAG AGACAGCTTA GTGAGGATGG GAAGCAGCTG 
CGGCGGGGGA GCCTGGGAGG AGCCCTCACA GGGAGGTACC TCCTTCCAAA CCCGGTAGCA 
GGACAGGCCT GGCCTGCTTC GGCGGAGACG TCCAACCTCG TGCGCATGCG CAGCCAGGCC 
CTTGGCCAAT CGGCTCCCTC GCTCACAGCC AGCTTGAAGG AGCTGAGCCT CCCCCGAAGA 
GGAAGTCTGG TAGATTCCCA GAAGTGGAAT TGCTTGGTCA AACGTTGCCG AACAAGCAAC 
CGGAAGAGTT TGATAGGCAA TGGCCAGTCT CCAGCACTGC CTCGACCACA CTCACCTCTC 
TCTGCTCATG CAGGAAATAG CCCTCAAGAC AGTCCAAGGA ATTTCTCCCC CAGTGCCTCA 
GCCCATTTCT CATTTGCGAG GAGAAATGAC AGAACGGATG GGCGCCGCTG GTCTCTGGCT 
TCTCTCCCTT CCTCAGGCTA TGGGACAAAT ACCCCAAGCT CCACCGTCTC CTCATCCTGT 
TCTTCCCAGG AGAAGTTGCA CCAGCTACCA TACCAGCCAA CCCCAGATGA ATTACACTTC 
TTATCCAAAC ACTTCTGCAC AACAGAAAGT ATCGCCACTG AGAACCGGTG CAGAAACACA 
CCCATGCGTC CACGTTCCCG GAGTCTCAGC CCTGGACGGT CCCCCGCCTG CTGTGACCAT 
GAAATAATTA TGATGAACCA TGTCTACAAA GAAAGGTTCC CAAAGGCCAC AGCTCAGATG 
GAAGAGCGTC TGAAGGAGAT CATCACCAGC TACTCTCCAG ACCATGTTCT CCCCTTGGCA 
GATGGGGTAC TTAGTTTCAC TCACCATCAG ATCATTGAGC TGGCTCGGGA CTGTTTGGAT 
AAATCTCACC AGGGTCTCAT CACGTCGAGA TACTTTTTTG AGTTGCAGCA CAAACTGGAC 
AAGTTGCTCC AGGAGGCTCA CGATCGCTCT GAAAGTGGAG AACTGGCATT TATCAAGCAA 
CTAGTCCGAA AGATCCTAAT TGTCATTGCC CGCCCCGCTC GGTTATTGGA GTGTTTGGAA 
TTTGATCCTG AAGAATTTTA TTACCTATTG GAAGCTGCAG AAGGCCATGC CAAAGAAGGC 
CAAGGAATCA AAACTGACAT CCCTAGGTAT ATCATCAGCC AGCTGGGACT CAATAAGGAC 
CCTTTAGAAG AAATGGCTCA GTTGGGGAAT TACGACAGTA GGACAGCAGA GACACCAGAG 
ATGGATGAGT CAGTGAGTAG CTCAAATACT TCCCTGAGAC TTCGAAGGAA ACCCCGAGAG 
AGTGATTTTG AAACAATTAA ATTGATCAGC AATGGAGCCT ACGGGGCAGT CTACTTTGTT 
CGGCACAAAG AGTCCCGCCA GAGGTTTGCC ATGAAGAAGA TCAACAAGCA GAACCTCATC 
CTTCGGAACC AGATCCAGCA GGCCTTCGTG GAGCGAGACA TCCTGACTTT CGCAGAGAAC 
CCCTTTGTGG TCAGCATGTA TTGCTCCTTT GAAACGAGGC GTCACTTATG CATGGTCATG 
GAGTATGTAG AAGGGGGAGA CTGTGCGACC CTAATGAAAA ACATGGGACC TCTCCCAGTT 
GATATGGCCA GAATGTATTT CGCCGAGACC GTCTTGGCCT TGGAGTACCT GCATAATTAC 
GGAATCGTAC ACAGGGACTT GAAGCCAGAC AACCTGTTGG TCACCTCCAT GGGGCACATA 
AAACTGACTG ACTTCGGCTT GTCTAAGGTG GGATTAATGA GCATGACCAC CAATCTCTAT 
GAAGGCCACA TAGAGAAGGA CGCTCGAGAG TTCTTAGATA AACAGGTCTG TGGTACACCT 
GAGTACATTG CCCCCGAGGT GATTCTGAGA CAGGGCTACG GGAAACCCGT GGACTGGTGG 
GCCATGGGCA TTATCCTCTA TGAATTTCTG GTCGGATGTG TGCCTTTCTT TGGGGACACT 
CCAGAAGAGC TATTTGGACA AGTCATCAGT GATGAAATCA ACTGGCCTGA AAAGGACGAG 
GCCCCTCCTC CAGACGCTCA GGAGCTGATT ACCTTGCTCC TCAGGCAGAA TCCGCTGGAG 
AGGCTGGGAA CAGGTGGAGC CTATGAAGTG AAGCAGCATC GTTTCTTCCG CTCCTTAGAC 
TGGAACAGTT TGCTGAGACA GAAGGCGGAA TTTATTCCCC AACTGGAATC GGAGGATGAC 
ACAAGTTATT TTGATACTCG GTCAGAGAAG TATCATCACA TGGAGACGGA GGAGGAGGAC 
GATACAAACG ATGAGGACTT CACCGTGGAG ATAAGGCAGT TTTCTTCCTG TTCACACAGG 
TTTTCAAAAG TTTTCAGCAG TATAGATCGC ATAACTCAAA ATTCAGGAGA AGACAAAGAT 
GACTCTGAGG ACAAGACCAA AAGCACAACG TTGCCATCCA CAGAGACACT CAGCTGGAGT 
TCCGAATACT CTGAAATGCA ACAGTTATCG ACCTCCAACT CTTCAGATAC TGAAAGCAAC 
AGGTGCAAAC TCAGCTCTGG CTTGCTCCCC AAGCTGGCTA TTTCGACAGA TGGGGAACAA 
GATGAGGCTG TCCCTTGCTC TGGAGACCCC AGAGAGGAGC CAGAGAAACC TGTCCCTCCC 
TCTGAGGAGT GTACTCAGGA GGAGCCCGAG GTCACCACCC CAGCCAGCAC CATCAGCAGT 
TCCACACTGT CAGTTGGCAG TTTTTCAGAG CACTTGGATC AGATAAATGG GCGAAGCGAG 
TGTGTGGACA GTACAGATAA TTCCTCAAAG CCATCCAGTG AACCCACTTC TCACGTGGCT 
CGACAGCGCT TAGAAAGCAC AGAGAAAAAG AAAATTTCTG GGAAAGTCAC AAAGTCCCTC 
TCGGCCAGTG CTCTGTCCCT CATGATCCCA GGAGATATGT TCGCTGTATC TCCATTGGGA 
AGCCCAATGT CCCCACACTC CCTGTCTTCA GACCCTTCTT CTTCACGGGA TTCCTCTCCC 
AGCCGAGACT CTTCTGCAGC ATCTGCCAGT CCGCATCAGC CCATTGTCAT CCACAGCTCA 
GGCAAGAACT ATGGGTTCAC CATCCGTGCT ATCCGCGTGT ACGTGGGGGA CAGTGACATC 
TACACAGTGC ACCATATCGT CTGGAACGTA GAAGAAGGAA GTCCCGCATA CCAGGCAGGA 
CTGAAGGCCG GGGATCTGAT CACACACATC AACGGAGAGC CGGTGCACGG CCTCGTCCAC 
ACGGAAGTTA TCGAGCTCCT GCTGAAGAGT GGGAATAAGG TGTCTATCAC CACTACTCCA 
TTTGAAAACA CATCAATCAA AACGGGACCA GCCAGGAGAA ACAGTTACAA GGGCCGGATG 
GTGAGACGAA GCAAGAAGTC CAAGAAGAAG GAGAGTCTAG AAAGGAGGAG ATCTCTCTTC 
AAGAAGCTGG CCAAGCAGCC TTCTCCTTTG CTCCACACCA GCCGAAGTTT CTCCTGCTTA 
AACCGGTCCC TGTCATCTGG AGAGAGCCTC CCGGGTTCCC CAACTCACAG CTTGTCCCCG 
AGGTCTCCAA CACCCAGTTA TCGTTCTACT CCCGATTTTC CGTCAGGTAC AAATTCCTCC 
CAGAGCAGCT CCCCAAGTTC AAGTGCCCCC AATTCTCCAG CAGGTTCAGG GCACATCCGG 
CCCAGCACCC TCCATGGCCT GGCTCCCAAA CTCAGCGGGC AGCGATACCG CTCTGGAAGA 
CGGAAGTCGG CTGGCAGCAT CCCTCTCTCC CCGCTGGCCA GGACACCCTC TCCCACTCCA 
CAGCCTACCT CTCCTCAGCG TTCACCATCC CCACTGTTGG GACACTCACT GGGCAATGCC 
AAGATCACTC AGGCCTTTCC TAGCAAGATG CACTCTCCCC CCACAATCGT CAGACACATC 
GTGAGGCCCA AGAGTGCAGA GCCGCCCCGC TCCCCACTGC TGAAACGGGT GCAGTCGGAG 
GAAAAGTTGT CACCCTCCTA TGGCAGTGAC AAGAAGCTTC TGTGCTCCCG CAAGCATAGC 
CTAGAGGTGA CACAAGAGGA GGTACAGAGG GAGCAGTGTC AGCGGGAAGT GACACTGCAG 
AGCCTGGAAG AGAATGTGTG TGACGCTCCT TCCCTCAGTC GGGCCAGGCC AGTGGAGCAA 
GGCTGTCTGA AACGCCCCGT GTCCCGGAAG GTGGGCAGGC AAGAGTCTGT GGATGACCTG 
GACCGGGACA AGCTGAAAGC CAAGGTTGTC GTAAAGAAAC CAGAAGAGAA ACATGAATCG 
CACCAGAAAC CTCACAGCCT TGGTGGTGAT TCGGAAAGCT ATGCTCTCTT CAGGCTAGAG 
GAGAGAGAGA AAAAAGTGTA CTCCAAGGGG TTGGAAAGGT CAGGCCATTT TGAAAACACA 
TCAGCAGAGT TGCCTTCTGT GGGCAGCCTG CTGAAGGACA CTCTTCACAA GCAGGCCAGC 
GTGAGGGCCA GCGAGGGGGT GACCTCAGAC GGGGCAGCTT GCAGCCTGAC ACCAGGGGAG 
CACAGCCAGT CTCTAGGTGA CTTTAAGCGG GCCTCAGCTT CTGGCATTCT CCATGATAGT 
GTGTGCCCCA TCTCTGATAG GCCTGCTCCT GGAAAGGTTG AATACTCGGA GAAGGCCTCT 
CAGGCCAAAG AGCTCCTTCG AAGTGAAAAA CTAGACAGCA AGCTGGCCAA TATTGATTAC 
CTCAGAAAGA AAATGTCATT GGATGACAAA GATGACAGCC ACTGTGCCAT CCTGAAACCC 
AAGATAACAT CTAGCGCCCA TGAATGTCTG CCAGGGAACC CCATACGGCC CATGGCAGGG 
CAACAAGAGA CCCCGCCAGC CTCTGAAAAC CGGGCATTCA TCAACAGTAC CCACACACCT 
CAGATGAGTG CAGTTTCCTT TGTTCCTCTC AAAGCCTTAG CTGGCCGGGT AGAGAACGGA 
GGGGAGAAAG CAGGCTTAGC TGCTCCCGAG TCCCCTGTCA GGAAGAGCCC CTCCGAGTAT 
AAGCTAGAGG GCAGGTCAGT TTCATGTCTC AAGCCGATCG AGGGCACACT GGACATTGCT 
CTCCTGTCTG GACCTCACGC CTCCAAAACA GAGTTGCTTT CCCCAGAGCC TGCACAGAGT 
CCCAGCCCAG GCATCAACGT GGGACCATGT GTGCCACTAG CTCTTCCTGG GAGCAGTGGG 
AAAAAGGGAG ACTCCACCAG CCTGAGAGAG CCTTCCTCAG CCAACTTAAA AGTAAATAAA 
TCTTATCTGC TGGAGCCTCG GTTCCTACCC CCGAGCCGGG CTCTCCAGGA CTCTCTCGCA 
GCCTCTGGGC CAGAACCGAA GTCAAAGCCG GAAAGGAAGC TCATTCATCC TTCTGCCAGG 
AGCCCAGCAA CTGTCACAGA GAGCAATCTT CAGCAGAAAG AGGGTGGTCC CGCCACACAC 
CAAGACCGCT CCACTGACAC CAGGAACCTC CCTGGCCCAG GGCAGACCCT ACACAATGTG 
GACCTACCCA GGCTGTGTAC ACGTGCCCCA CTCCCACCGG AAGGGACGCC CGCAAAGGAG 
AAGCCATGTC TGAAGGAACC CTCTGCCAAG GTGAAAAGCG AGTGGTCTGC CGTGAGGGAT 
GACGGACACA GAGATCCCTG TGCGAAGCTG TGCCCGGCAG AGACTGGTAA AGCCAGCGAC 
AGTTCCAAAC CCCTGCCTTC CGGGGGGAGG ACCCAACCCG ATTTCTACAA GCAGACCCAG 
ACTTCGGAGA AAGCATGGGC GCATGCAAAA ACAAACCACA AAGATAGCCA AGATGAGGTG 
AAGTCCCTGG CCAGGGAGGA CTCAGCTTCA CTTTTATATG AAAAGGAGAT AGGCCGGGCA 
CGAAAAGGTC CTGAACCCAA ACCGGAAGTT CCTGCTACCC GGTGCCCTCC TCAGCCACCA 
GGAATTGAGG GTGAGAAGCG AGAAAAGCTC TCCGCTGCCC CCTCTTTGCA GAAACAGGCT 
CCCAAAGAGC CAGACAGGAA GGAACAGACT TCGCAAAGGC CTGGAGGTAG TGGCCCTCAA 
CAACCCCCAC CCACCAAAGA GCTGTCTAAC TCAGCATCCT GGCAGCACGG CAGTTCTCCG 
AGTCACACTT TAAAGAAGGA GCCCGGGACC AAAGCTGCCG CTGCAGAACC AAGCACCAGC 
CTTCATGACA CTCCCCGATC TGCTACAGCC ACCACCACTG CCATTGCCAC CACCACCACT 
ACCACCAGTG CCGGGCACAG TGACTGCAGT AGCCATAAGG CCCGGCCTGG CCCTGACCCC 
AGCCCTTCAA AGTCTAAGCA CCAAGACAGG TCCCTCTCCT CACAGAAGCT GAGTGCTGGC 
TCTGCAAAAG GCAAAGAGCC TGTCACTCAA CCCCTGGGTG GTTCCATCAG AGAAGGCAAG 
GGTGGCAGCA AGGGTCCAGT GGACACATTT TCTGCTGTCC TGACCACCCA GGGCAAAGCA 
AGTGATGTGC TTGTGCAGGG AGAAGGTCGG GTCTCAATCA TTGTCCACAC TGAAGAGTGT 
CCTCTCGATG CCAAACTGAA AAACACCAAT GGAGGGTGTC CCCCAGAGAT GCAGGCGAAG 
CATCCACCCA GACAAGGACA TCTCAGTGAA GCAGCAGACC AGAAGCCACT CATTGCTGGT 
GAGAAGCAAA GCCCGTCTCC AAAGCATCCC AAACCATCCA CTGTGAAAGA TTACCCCAGT 
CTGTGCAGAC AGACAGACAG AAGCCCAAGC CATCAGGCTA CCACTGGGGA CAGGAAGGCA 
GAAGGAAAGA AATGCACAGA CGCACTTTAT GTCGCAGCCC CAGAGGGCTA CAAGCCAGAG 
GCCAGCCCTT CTCTCCACCA CGGCGAGACC GGACTCAGAG GCTCAGAGAG GCCACCCATG 
GGCATGGGGA AGGGCTTCTC TGAGCCCAAG GGGAAAGGGC CAGGTCCCCA GAAGTCACTG 
GCTGAAACAG GCAAGCCCAG CGGTATGAAA AGGTCACCCT CTGCCACCGT GCAGAGCTCT 
CTCCGCTCAG CTGCCCCCCC AGAAAAGTCT CTGAGTTACT CAGCCAGCTT TCCCGAGGCC 
CAGCCTGGAG TGCGAGAGGT CCCTGCAGCC AACAGCAGCC CCTCATCTGC CAAGGCTACA 
GGGGGGACCT CAGAGTTCCC AGCCCCCAGC AGCAGGGACC ACAGGAAGCT TCAGTCTGGA 
GGAGACGGCC GAAGCCAAAT GATAAAGAGT GACTCTCTGC CCTCCTTCCG CCTCTCCACC 
TCTGCTCTGG AGTCACATTT CCAGGATCCA CAGGTGCCCA TCGCATCAGG CCACCGAGGC 
AGGGCACTGT CAGTAACTGC TGCCACAGGA GAACCCAAAG GGAGAGAGCT CGCCCAGCCT 
CCCCCAGTCA GGAAACAGAA TGCGTGCAGA GAGGCGACCA GAGCACCCCC AGCCCCAAGC 
ACAGATCGCT CCCTCCCTCT TTCCTCAGAG AAAGACTTCG TGGTTCGGCA GAGAAGGGGC 
AAGGAGACCT TAAGGAGCAG TCCTCACAAA AAGGCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of Presenilin 1 and 2 interacting proteins in mouse cerebral cortex during development.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience38138-46(2014 Nov)
Kumar A,Thakur MK

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Nagase T,Ohara O,Koga H

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Cloning of the genes encoding two murine and human cochlear unconventional type I myosins.
Genomics40(2)332-41(1997 Mar)
Crozet F,el Amraoui A,Blanchard S,Lenoir M,Ripoll C,Vago P,Hamel C,Fizames C,Levi-Acobas F,Dep?tris D,Mattei MG,Weil D,Pujol R,Petit C

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Mast4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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