Ddx11 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx11
Entrez Gene ID	320209
Full Name	DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11
Synonyms	4732462I11Rik,CHL1,CHLR1,KRG2,essa15a
General protein information	Preferred Names DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 Names ATP-dependent DNA helicase DDX11 probable ATP-dependent DNA helicase DDX11 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae) probable ATP-dependent RNA helicase DDX11
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E1.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006524410.4	XP_006524473.1	ATP-dependent DNA helicase DDX11 isoform X2
NM_001003919.1	NP_001003919.1	probable ATP-dependent RNA helicase DDX11
NM_001003919.2	NP_001003919.1	ATP-dependent DNA helicase DDX11 isoform 1
XM_006524408.3	XP_006524471.1	probable ATP-dependent DNA helicase DDX11 isoform X1
XM_006524411.3	XP_006524474.1	probable ATP-dependent DNA helicase DDX11 isoform X3
XM_006524409.3	XP_006524472.1	ATP-dependent DNA helicase DDX11 isoform X1
NM_001348292.1	NP_001335221.1	ATP-dependent DNA helicase DDX11 isoform 2
XM_006524410.3	XP_006524473.1	probable ATP-dependent DNA helicase DDX11 isoform X2

Pathways from BioSystems

Homologies

	LOC717040	XP_001114510.2
Mus musculus (house mouse)	Ddx11	NP_001003919.1
Drosophila melanogaster (fruit fly)	CG11403	NP_569898.1
Caenorhabditis elegans (roundworm)	chl-1	NP_499295.2
Kluyveromyces lactis	KLLA0F27181g	XP_456289.1
Canis lupus familiaris (dog)	DDX11	XP_005637382.1
Bos taurus (cattle)	LOC506293	XP_005207335.1
Saccharomyces cerevisiae (baker's yeast)	CHL1	NP_015317.1
Magnaporthe oryzae (rice blast fungus)	MGG_13542	XP_003716432.1
Schizosaccharomyces pombe (fission yeast)	chl1	NP_594977.1
Arabidopsis thaliana (thale cress)	AT1G79890	NP_178107.1
Pan troglodytes (chimpanzee)	DDX11	XP_003313865.1
Rattus norvegicus (Norway rat)	Ddx11	XP_006245677.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001537	XP_321585.4
Eremothecium gossypii	AGOS_AGL010W	NP_986656.1
Xenopus tropicalis (tropical clawed frog)	ddx11	NP_001164296.1
Homo sapiens (human)	DDX11	NP_085911.2
Gallus gallus (chicken)	DDX11	XP_416375.4
Danio rerio (zebrafish)	ddx11	NP_001007320.1
Neurospora crassa	NCU11409	XP_001728521.1

Gene Ontology
Bibliography

Related articles in PubMed

The ENU-induced cetus mutation reveals an essential role of the DNA helicase DDX11 for mesoderm development during early mouse embryogenesis.
Cota CD, Garc?a-Garc?a MJ
Developmental dynamics : an official publication of the American Association of Anatomists241(8)1249-59(2012 Aug)

Mammalian ChlR1 has a role in heterochromatin organization.
Inoue A, Hyle J, Lechner MS, Lahti JM
Experimental cell research317(17)2522-35(2011 Oct)

Loss of ChlR1 helicase in mouse causes lethality due to the accumulation of aneuploid cells generated by cohesion defects and placental malformation.
Inoue A, Li T, Roby SK, Valentine MB, Inoue M, Boyd K, Kidd VJ, Lahti JM
Cell cycle (Georgetown, Tex.)6(13)1646-54(2007 Jul)

Analysis of mouse embryonic patterning and morphogenesis by forward genetics.
Garc?a-Garc?a MJ, Eggenschwiler JT, Caspary T, Alcorn HL, Wyler MR, Huangfu D, Rakeman AS, Lee JD, Feinberg EH, Timmer JR, Anderson KV
Proceedings of the National Academy of Sciences of the United States of America102(17)5913-9(2005 Apr)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ChlR1 is essential for embryonic development and the prevention of aneuploidy in mammals.
Title: Loss of ChlR1 helicase in mouse causes lethality due to the accumulation of aneuploid cells generated by cohesion defects and placental malformation.

The following Ddx11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu53689	XM_006524411.3 Latest version!	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu53687	NM_001348292.1 Latest version!	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu53687	XM_006524409.3 Latest version!	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu53687	XM_006524408.3 Latest version!	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu10542	NM_001003919.2 Latest version!	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$517.30 $739.00
OMu53688	XM_006524410.4 Latest version!	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu10542	NM_001003919.1	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$517.30 $739.00
OMu53688	XM_006524410.3	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx11 ( XM_006524410.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524473.1 Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu53688
Clone ID Related Accession (Same CDS sequence)	XM_006524410.4 , XM_006524410.3
Accession Version	XM_006524410.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent DNA helicase DDX11 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524410.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGG TGAGGAGGAG GAAGCGAGAG 
GAGCGACTGA AGGAGGTCTG CCAGGATGGG AGGCTCAGGT TTGCAGCCAA GCGCACGAAA 
CATGAGGAAG AGGAGACTGA GGCTCTCCTG CGCCTCAGCA GGGAGATGCT GGATGCAGGG 
ACAGGACCAG AGCAGCTGGA GCAGCTGGAG TGTGGGGAGG AGCACCTGGT TCTGGCAGAA 
TATGAGAGTG ATGAGGAGAG AAGAGGCAGC AGGGTGGACG AGGCTGAGGA TGACTTGGAG 
GAAGAACATA TAACCAAGAT TTATTACTGC AGTCGGACAC ACTCACAGCT GGCCCAGTTT 
GTGCGGGAAG TACTGAAGAG CCCTTTTGGC AAGGAGACCC GGCTAGTCTC ACTTGGCTCT 
AGGCAGACCC TTTGTGTGAA TGAAGATGTG AAAAACCTGG GTTCTGTGCA ACTTATGAAT 
GACCGCTGTG TGGACATGCA AAGAAGCAAA CGCGAAAAGA ACGGGACTGG GGAAGACAAG 
CCAAAGAGAA AGAGACAGAA GATCCAGACC TCTTGCCCCT TCTACAACCA CGAGCAGATG 
GAGCTTCTCC GGGATGAGAT TCTGCTGGAA GTGAAGGACA TGGAACAACT TGTGGCCCTT 
GGGAAGGAGG CCCGGGCCTG CCCCTATTAC GGAAGCCGCT TTGCCATCCC TGCAGCCCAG 
CTAGTGGTGC TACCCTACCC AATGTTGCTG CATGCAGCTA CCCGACAGGC TGCGGGAATC 
CGGCTACAAG GTCAGGTGGT CATTATTGAC GAGGCTCACA ACCTGATCGA CACCATCACC 
AACATCCACA GCACAGAGGT CAATGGTTCC CAGCTCTGCC AGGCCCATTC CCAGTTGCTC 
CAGTACATGG AAAGATACAG GAAGCGTTTA AAGGCCAAGA ACTTGATGTA CATCAAGCAG 
ATCCTGTACT TGCTGGAGAA GTTTGTGGCT GTTTTGGGAG GTAATGTTAA GCAAAATCCT 
ACTACACAGA GCCTGTCTCA AACAGGGTCT GAGCTGAAGA GCATCAACGA CTTTCTCTTT 
CAGAGCCAGG TGGACAACAT CAACCTCTTC AAGGTGCAGC GGTACTTGGA GAAGAGCATG 
CTCAGCAGGA AGCTCTTTGG CTTCACAGAA TGTTTCGGAG TGGTTCTTCC ATCCCTCTCA 
GACTCTCAGG AAAACCGAGG TCTGGCTGGC TTCCAGCAGT TCCTGAAGAG CCTGCAGTCA 
GGGCCTACTG AGGACTCCCC GGAGGAGGGC CAGGCTGTAG CCCTGCGGCC TGCCTCTCCA 
CTGATGCACA TCGAGGCCTT CCTGGCAGCT CTCACCACAG CCAATCAGGA TGGCAGGGTT 
ATTGTGAACC GCCAAGGCAG TGTGGGTCAG AGCAGCCTTA AATTCTTACT CTTGAACCCA 
GCTGTGCACT TTGCCCAGGT GGTGAAGGAA TGCCGAGCAG TGGTCATTGC AGGTGGCACC 
ATGCAGCCGA CTTCTCTTAC ATTCACTACA AGAGCACACA ACCCCAGGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524473.1
CDS	192..2105
Translation	MADENQEIGGIHFPFPFPPYPIQKDFMAELYKVLEGGKIGIFESPTGTGKSLSLICGALSWLRDFEKKKLQAEALLLAPGSGPPSSEKNSLLTSSSCQEPTDTPRPAGEPDWVTEFVQKKEERDLVERLREEQVRRRKREERLKEVCQDGRLRFAAKRTKHEEEETEALLRLSREMLDAGTGPEQLEQLECGEEHLVLAEYESDEERRGSRVDEAEDDLEEEHITKIYYCSRTHSQLAQFVREVLKSPFGKETRLVSLGSRQTLCVNEDVKNLGSVQLMNDRCVDMQRSKREKNGTGEDKPKRKRQKIQTSCPFYNHEQMELLRDEILLEVKDMEQLVALGKEARACPYYGSRFAIPAAQLVVLPYPMLLHAATRQAAGIRLQGQVVIIDEAHNLIDTITNIHSTEVNGSQLCQAHSQLLQYMERYRKRLKAKNLMYIKQILYLLEKFVAVLGGNVKQNPTTQSLSQTGSELKSINDFLFQSQVDNINLFKVQRYLEKSMLSRKLFGFTECFGVVLPSLSDSQENRGLAGFQQFLKSLQSGPTEDSPEEGQAVALRPASPLMHIEAFLAALTTANQDGRVIVNRQGSVGQSSLKFLLLNPAVHFAQVVKECRAVVIAGGTMQPTSLTFTTRAHNPRL

Target ORF information:

RefSeq Version	XM_006524410.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524410.3

ORF Insert Sequence:

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGG TGAGGAGGAG GAAGCGAGAG 
GAGCGACTGA AGGAGGTCTG CCAGGATGGG AGGCTCAGGT TTGCAGCCAA GCGCACGAAA 
CATGAGGAAG AGGAGACTGA GGCTCTCCTG CGCCTCAGCA GGGAGATGCT GGATGCAGGG 
ACAGGACCAG AGCAGCTGGA GCAGCTGGAG TGTGGGGAGG AGCACCTGGT TCTGGCAGAA 
TATGAGAGTG ATGAGGAGAG AAGAGGCAGC AGGGTGGACG AGGCTGAGGA TGACTTGGAG 
GAAGAACATA TAACCAAGAT TTATTACTGC AGTCGGACAC ACTCACAGCT GGCCCAGTTT 
GTGCGGGAAG TACTGAAGAG CCCTTTTGGC AAGGAGACCC GGCTAGTCTC ACTTGGCTCT 
AGGCAGACCC TTTGTGTGAA TGAAGATGTG AAAAACCTGG GTTCTGTGCA ACTTATGAAT 
GACCGCTGTG TGGACATGCA AAGAAGCAAA CGCGAAAAGA ACGGGACTGG GGAAGACAAG 
CCAAAGAGAA AGAGACAGAA GATCCAGACC TCTTGCCCCT TCTACAACCA CGAGCAGATG 
GAGCTTCTCC GGGATGAGAT TCTGCTGGAA GTGAAGGACA TGGAACAACT TGTGGCCCTT 
GGGAAGGAGG CCCGGGCCTG CCCCTATTAC GGAAGCCGCT TTGCCATCCC TGCAGCCCAG 
CTAGTGGTGC TACCCTACCC AATGTTGCTG CATGCAGCTA CCCGACAGGC TGCGGGAATC 
CGGCTACAAG GTCAGGTGGT CATTATTGAC GAGGCTCACA ACCTGATCGA CACCATCACC 
AACATCCACA GCACAGAGGT CAATGGTTCC CAGCTCTGCC AGGCCCATTC CCAGTTGCTC 
CAGTACATGG AAAGATACAG GAAGCGTTTA AAGGCCAAGA ACTTGATGTA CATCAAGCAG 
ATCCTGTACT TGCTGGAGAA GTTTGTGGCT GTTTTGGGAG GTAATGTTAA GCAAAATCCT 
ACTACACAGA GCCTGTCTCA AACAGGGTCT GAGCTGAAGA GCATCAACGA CTTTCTCTTT 
CAGAGCCAGG TGGACAACAT CAACCTCTTC AAGGTGCAGC GGTACTTGGA GAAGAGCATG 
CTCAGCAGGA AGCTCTTTGG CTTCACAGAA TGTTTCGGAG TGGTTCTTCC ATCCCTCTCA 
GACTCTCAGG AAAACCGAGG TCTGGCTGGC TTCCAGCAGT TCCTGAAGAG CCTGCAGTCA 
GGGCCTACTG AGGACTCCCC GGAGGAGGGC CAGGCTGTAG CCCTGCGGCC TGCCTCTCCA 
CTGATGCACA TCGAGGCCTT CCTGGCAGCT CTCACCACAG CCAATCAGGA TGGCAGGGTT 
ATTGTGAACC GCCAAGGCAG TGTGGGTCAG AGCAGCCTTA AATTCTTACT CTTGAACCCA 
GCTGTGCACT TTGCCCAGGT GGTGAAGGAA TGCCGAGCAG TGGTCATTGC AGGTGGCACC 
ATGCAGCCGA CTTCTCTTAC ATTCACTACA AGAGCACACA ACCCCAGGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx11 ( XM_006524411.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524474.1 Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu53689
Clone ID Related Accession (Same CDS sequence)	XM_006524411.3
Accession Version	XM_006524411.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent DNA helicase DDX11 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524411.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGG TGAGGAGGAG GAAGCGAGAG 
GAGCGACTGA AGGAGGTCTG CCAGGATGGG AGGCTCAGGT TTGCAGCCAA GCGCACGAAA 
CATGAGGAAG AGGAGACTGA GGCTCTCCTG CGCCTCAGCA GGGAGATGCT GGATGCAGGG 
ACAGGACCAG AGCAGCTGGA GCAGCTGGAG TGTGGGGAGG AGCACCTGGT TCTGGCAGAA 
TATGAGAGTG ATGAGGAGAG AAGAGGCAGC AGGGTGGACG AGGCTGAGGA TGACTTGGAG 
GAAGAACATA TAACCAAGAT TTATTACTGC AGTCGGACAC ACTCACAGCT GGCCCAGTTT 
GTGCGGGAAG TACTGAAGAG CCCTTTTGGC AAGGAGACCC GGCTAGTCTC ACTTGGCTCT 
AGGCAGACCC TTTGTGTGAA TGAAGATGTG AAAAACCTGG GTTCTGTGCA ACTTATGAAT 
GACCGCTGTG TGGACATGCA AAGAAGCAAA CGCGAAAAGA ACGGGACTGG GGAAGACAAG 
CCAAAGAGAA AGAGACAGAA GATCCAGACC TCTTGCCCCT TCTACAACCA CGAGCAGATG 
GAGCTTCTCC GGGATGAGAT TCTGCTGGAA GTGAAGGACA TGGAACAACT TGTGGCCCTT 
GGGAAGGAGG CCCGGGCCTG CCCCTATTAC GGAAGCCGCT TTGCCATCCC TGCAGCCCAG 
CTAGTGGTGC TACCCTACCC AATGTTGCTG CATGCAGCTA CCCGACAGGC TGCGGGAATC 
CGGCTACAAG GTCAGGTGGT CATTATTGAC GAGGCTCACA ACCTGATCGA CACCATCACC 
AACATCCACA GCACAGAGGT CAATGGTTCC CAGATGCCCA TCACCTTGAT GGCACCCGTG 
TGTCCTGGGC TGGCATTTCC TAGATCTCTA CAGAGATCCT CTGAATCCTA TCCTGGGCTG 
CCTTCAGGCT GTTCTGTTTT CTCTCCAGCT CTGCCAGGCC CATTCCCAGT TGCTCCAGTA 
CATGGAAAGA TACAGGAAGC GTTTAAAGGC CAAGAACTTG ATGTACATCA AGCAGATCCT 
GTACTTGCTG GAGAAGTTTG TGGCTGTTTT GGGAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524474.1
CDS	193..1671
Translation	MADENQEIGGIHFPFPFPPYPIQKDFMAELYKVLEGGKIGIFESPTGTGKSLSLICGALSWLRDFEKKKLQAEALLLAPGSGPPSSEKNSLLTSSSCQEPTDTPRPAGEPDWVTEFVQKKEERDLVERLREEQVRRRKREERLKEVCQDGRLRFAAKRTKHEEEETEALLRLSREMLDAGTGPEQLEQLECGEEHLVLAEYESDEERRGSRVDEAEDDLEEEHITKIYYCSRTHSQLAQFVREVLKSPFGKETRLVSLGSRQTLCVNEDVKNLGSVQLMNDRCVDMQRSKREKNGTGEDKPKRKRQKIQTSCPFYNHEQMELLRDEILLEVKDMEQLVALGKEARACPYYGSRFAIPAAQLVVLPYPMLLHAATRQAAGIRLQGQVVIIDEAHNLIDTITNIHSTEVNGSQMPITLMAPVCPGLAFPRSLQRSSESYPGLPSGCSVFSPALPGPFPVAPVHGKIQEAFKGQELDVHQADPVLAGEVCGCFGR

Target ORF information:

RefSeq Version	XM_006524411.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524411.3

ORF Insert Sequence:

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGG TGAGGAGGAG GAAGCGAGAG 
GAGCGACTGA AGGAGGTCTG CCAGGATGGG AGGCTCAGGT TTGCAGCCAA GCGCACGAAA 
CATGAGGAAG AGGAGACTGA GGCTCTCCTG CGCCTCAGCA GGGAGATGCT GGATGCAGGG 
ACAGGACCAG AGCAGCTGGA GCAGCTGGAG TGTGGGGAGG AGCACCTGGT TCTGGCAGAA 
TATGAGAGTG ATGAGGAGAG AAGAGGCAGC AGGGTGGACG AGGCTGAGGA TGACTTGGAG 
GAAGAACATA TAACCAAGAT TTATTACTGC AGTCGGACAC ACTCACAGCT GGCCCAGTTT 
GTGCGGGAAG TACTGAAGAG CCCTTTTGGC AAGGAGACCC GGCTAGTCTC ACTTGGCTCT 
AGGCAGACCC TTTGTGTGAA TGAAGATGTG AAAAACCTGG GTTCTGTGCA ACTTATGAAT 
GACCGCTGTG TGGACATGCA AAGAAGCAAA CGCGAAAAGA ACGGGACTGG GGAAGACAAG 
CCAAAGAGAA AGAGACAGAA GATCCAGACC TCTTGCCCCT TCTACAACCA CGAGCAGATG 
GAGCTTCTCC GGGATGAGAT TCTGCTGGAA GTGAAGGACA TGGAACAACT TGTGGCCCTT 
GGGAAGGAGG CCCGGGCCTG CCCCTATTAC GGAAGCCGCT TTGCCATCCC TGCAGCCCAG 
CTAGTGGTGC TACCCTACCC AATGTTGCTG CATGCAGCTA CCCGACAGGC TGCGGGAATC 
CGGCTACAAG GTCAGGTGGT CATTATTGAC GAGGCTCACA ACCTGATCGA CACCATCACC 
AACATCCACA GCACAGAGGT CAATGGTTCC CAGATGCCCA TCACCTTGAT GGCACCCGTG 
TGTCCTGGGC TGGCATTTCC TAGATCTCTA CAGAGATCCT CTGAATCCTA TCCTGGGCTG 
CCTTCAGGCT GTTCTGTTTT CTCTCCAGCT CTGCCAGGCC CATTCCCAGT TGCTCCAGTA 
CATGGAAAGA TACAGGAAGC GTTTAAAGGC CAAGAACTTG ATGTACATCA AGCAGATCCT 
GTACTTGCTG GAGAAGTTTG TGGCTGTTTT GGGAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx11 ( NM_001348292.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001335221.1 Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu53687
Clone ID Related Accession (Same CDS sequence)	XM_006524408.3 , XM_006524409.3 , NM_001348292.1
Accession Version	NM_001348292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	ATP-dependent DNA helicase DDX11 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC079656.1, AK152868.1 and CT025671.10. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGG TGAGGAGGAG GAAGCGAGAG 
GAGCGACTGA AGGAGGTCTG CCAGGATGGG AGGCTCAGGT TTGCAGCCAA GCGCACGAAA 
CATGAGGAAG AGGAGACTGA GGCTCTCCTG CGCCTCAGCA GGGAGATGCT GGATGCAGGG 
ACAGGACCAG AGCAGCTGGA GCAGCTGGAG TGTGGGGAGG AGCACCTGGT TCTGGCAGAA 
TATGAGAGTG ATGAGGAGAG AAGAGGCAGC AGGGTGGACG AGGCTGAGGA TGACTTGGAG 
GAAGAACATA TAACCAAGAT TTATTACTGC AGTCGGACAC ACTCACAGCT GGCCCAGTTT 
GTGCGGGAAG TACTGAAGAG CCCTTTTGGC AAGGAGACCC GGCTAGTCTC ACTTGGCTCT 
AGGCAGACCC TTTGTGTGAA TGAAGATGTG AAAAACCTGG GTTCTGTGCA ACTTATGAAT 
GACCGCTGTG TGGACATGCA AAGAAGCAAA CGCGAAAAGA ACGGGACTGG GGAAGACAAG 
CCAAAGAGAA AGAGACAGAA GATCCAGACC TCTTGCCCCT TCTACAACCA CGAGCAGATG 
GAGCTTCTCC GGGATGAGAT TCTGCTGGAA GTGAAGGACA TGGAACAACT TGTGGCCCTT 
GGGAAGGAGG CCCGGGCCTG CCCCTATTAC GGAAGCCGCT TTGCCATCCC TGCAGCCCAG 
CTAGTGGTGC TACCCTACCC AATGTTGCTG CATGCAGCTA CCCGACAGGC TGCGGGAATC 
CGGCTACAAG GTCAGGTGGT CATTATTGAC GAGGCTCACA ACCTGATCGA CACCATCACC 
AACATCCACA GCACAGAGGT CAATGGTTCC CAGCTCTGCC AGGCCCATTC CCAGTTGCTC 
CAGTACATGG AAAGATACAG GAAGCGTTTA AAGGCCAAGA ACTTGATGTA CATCAAGCAG 
ATCCTGTACT TGCTGGAGAA GTTTGTGGCT GTTTTGGGAG GTAATGTTAA GCAAAATCCT 
ACTACACAGA GCCTGTCTCA AACAGGGTCT GAGCTGAAGA GCATCAACGA CTTTCTCTTT 
CAGAGCCAGG TGGACAACAT CAACCTCTTC AAGGTGCAGC GGTACTTGGA GAAGAGCATG 
CTCAGCAGGA AGCTCTTTGG CTTCACAGAA TGTTTCGGAG TGGTTCTTCC ATCCCTCTCA 
GACTCTCAGG AAAACCGAGG TCTGGCTGGC TTCCAGCAGT TCCTGAAGAG CCTGCAGTCA 
GGGCCTACTG AGGACTCCCC GGAGGAGGGC CAGGCTGTAG CCCTGCGGCC TGCCTCTCCA 
CTGATGCACA TCGAGGCCTT CCTGGCAGCT CTCACCACAG CCAATCAGGA TGGCAGGGTT 
ATTGTGAACC GCCAAGGCAG TGTGGGTCAG AGCAGCCTTA AATTCTTACT CTTGAACCCA 
GCTGTGCACT TTGCCCAGGT GGTGAAGGAA TGCCGAGCAG TGGTCATTGC AGGTGGCACC 
ATGCAGCCGA TGTCTGACTT TCGGGAGCAG CTGCTGGCAT GTTCTGGAGT AGAAGCGGGG 
CGAGTGGTGG AGTTTTCCTG TGGTCACGTG ATCCCTCCAG ACAACATCTT GCCCCTTATT 
ATATGCAGTG GGCCCTCTAA CCAGCAGCTG GAATTCACCT ACCAGAGAAG AGAGCTGCCT 
CAGATGGTGG AGGAGACAGG CCGCATTCTC TGTAACTTGT GCAATGTGGT CCCTGGAGGT 
GTGGTATGCT TTCTCCCTTC TTATGAGTAC CTGCGCCAGG TACATGCTCA CTGGGACAAG 
ACTGGCCTGC TGACACGTTT GTCTGTCAGG AAAAAGATAT TTCAAGAGCC CAAAAGAGCG 
AGCCAGGTGG AGCAGGTGCT GATGGCGTAT TCCAAGTGCA TTATGAGCTG CAGTCACTCA 
GAAGGCCATC TGACAGGGGC CTTGCTCCTC TCTGTGGTTG GAGGGAAGAT GAGTGAAGGG 
ATCAATTTCT CTGATGACCT GGGCCGGTGT GTGGTGATGG TGGGGATGCC ATATCCCAAC 
ATTAAGTCTC CAGAGCTGCA AGAGAAGATG GCCTACTTGA ATCAGACACT TCCTAGGACA 
CAAGGTCAGC CCCTGCCAGG GACGGTATTG ATAGAGAATC TGTGTATGAA GGCTATAAAC 
CAATCCATAG GCAGGGCCAT TAGGCACCAG AGGGACTTTG CCAGCATTGT GCTCCTGGAT 
CACCGGTATG CGCGTCCTTC TATTCTGGCG AAGCTGCCAG CCTGGATACG AGACCGAGTG 
GAGGTCAAAG CTACCTTTGG GCCTGCATTT GCTGCTGTGC GGAAGTTTCA TCGAGAGAAG 
TCACACCCAA GTCTGGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335221.1
CDS	178..2898
Translation	MADENQEIGGIHFPFPFPPYPIQKDFMAELYKVLEGGKIGIFESPTGTGKSLSLICGALSWLRDFEKKKLQAEALLLAPGSGPPSSEKNSLLTSSSCQEPTDTPRPAGEPDWVTEFVQKKEERDLVERLREEQVRRRKREERLKEVCQDGRLRFAAKRTKHEEEETEALLRLSREMLDAGTGPEQLEQLECGEEHLVLAEYESDEERRGSRVDEAEDDLEEEHITKIYYCSRTHSQLAQFVREVLKSPFGKETRLVSLGSRQTLCVNEDVKNLGSVQLMNDRCVDMQRSKREKNGTGEDKPKRKRQKIQTSCPFYNHEQMELLRDEILLEVKDMEQLVALGKEARACPYYGSRFAIPAAQLVVLPYPMLLHAATRQAAGIRLQGQVVIIDEAHNLIDTITNIHSTEVNGSQLCQAHSQLLQYMERYRKRLKAKNLMYIKQILYLLEKFVAVLGGNVKQNPTTQSLSQTGSELKSINDFLFQSQVDNINLFKVQRYLEKSMLSRKLFGFTECFGVVLPSLSDSQENRGLAGFQQFLKSLQSGPTEDSPEEGQAVALRPASPLMHIEAFLAALTTANQDGRVIVNRQGSVGQSSLKFLLLNPAVHFAQVVKECRAVVIAGGTMQPMSDFREQLLACSGVEAGRVVEFSCGHVIPPDNILPLIICSGPSNQQLEFTYQRRELPQMVEETGRILCNLCNVVPGGVVCFLPSYEYLRQVHAHWDKTGLLTRLSVRKKIFQEPKRASQVEQVLMAYSKCIMSCSHSEGHLTGALLLSVVGGKMSEGINFSDDLGRCVVMVGMPYPNIKSPELQEKMAYLNQTLPRTQGQPLPGTVLIENLCMKAINQSIGRAIRHQRDFASIVLLDHRYARPSILAKLPAWIRDRVEVKATFGPAFAAVRKFHREKSHPSLV

Target ORF information:

RefSeq Version	NM_001348292.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348292.1

ORF Insert Sequence:

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGG TGAGGAGGAG GAAGCGAGAG 
GAGCGACTGA AGGAGGTCTG CCAGGATGGG AGGCTCAGGT TTGCAGCCAA GCGCACGAAA 
CATGAGGAAG AGGAGACTGA GGCTCTCCTG CGCCTCAGCA GGGAGATGCT GGATGCAGGG 
ACAGGACCAG AGCAGCTGGA GCAGCTGGAG TGTGGGGAGG AGCACCTGGT TCTGGCAGAA 
TATGAGAGTG ATGAGGAGAG AAGAGGCAGC AGGGTGGACG AGGCTGAGGA TGACTTGGAG 
GAAGAACATA TAACCAAGAT TTATTACTGC AGTCGGACAC ACTCACAGCT GGCCCAGTTT 
GTGCGGGAAG TACTGAAGAG CCCTTTTGGC AAGGAGACCC GGCTAGTCTC ACTTGGCTCT 
AGGCAGACCC TTTGTGTGAA TGAAGATGTG AAAAACCTGG GTTCTGTGCA ACTTATGAAT 
GACCGCTGTG TGGACATGCA AAGAAGCAAA CGCGAAAAGA ACGGGACTGG GGAAGACAAG 
CCAAAGAGAA AGAGACAGAA GATCCAGACC TCTTGCCCCT TCTACAACCA CGAGCAGATG 
GAGCTTCTCC GGGATGAGAT TCTGCTGGAA GTGAAGGACA TGGAACAACT TGTGGCCCTT 
GGGAAGGAGG CCCGGGCCTG CCCCTATTAC GGAAGCCGCT TTGCCATCCC TGCAGCCCAG 
CTAGTGGTGC TACCCTACCC AATGTTGCTG CATGCAGCTA CCCGACAGGC TGCGGGAATC 
CGGCTACAAG GTCAGGTGGT CATTATTGAC GAGGCTCACA ACCTGATCGA CACCATCACC 
AACATCCACA GCACAGAGGT CAATGGTTCC CAGCTCTGCC AGGCCCATTC CCAGTTGCTC 
CAGTACATGG AAAGATACAG GAAGCGTTTA AAGGCCAAGA ACTTGATGTA CATCAAGCAG 
ATCCTGTACT TGCTGGAGAA GTTTGTGGCT GTTTTGGGAG GTAATGTTAA GCAAAATCCT 
ACTACACAGA GCCTGTCTCA AACAGGGTCT GAGCTGAAGA GCATCAACGA CTTTCTCTTT 
CAGAGCCAGG TGGACAACAT CAACCTCTTC AAGGTGCAGC GGTACTTGGA GAAGAGCATG 
CTCAGCAGGA AGCTCTTTGG CTTCACAGAA TGTTTCGGAG TGGTTCTTCC ATCCCTCTCA 
GACTCTCAGG AAAACCGAGG TCTGGCTGGC TTCCAGCAGT TCCTGAAGAG CCTGCAGTCA 
GGGCCTACTG AGGACTCCCC GGAGGAGGGC CAGGCTGTAG CCCTGCGGCC TGCCTCTCCA 
CTGATGCACA TCGAGGCCTT CCTGGCAGCT CTCACCACAG CCAATCAGGA TGGCAGGGTT 
ATTGTGAACC GCCAAGGCAG TGTGGGTCAG AGCAGCCTTA AATTCTTACT CTTGAACCCA 
GCTGTGCACT TTGCCCAGGT GGTGAAGGAA TGCCGAGCAG TGGTCATTGC AGGTGGCACC 
ATGCAGCCGA TGTCTGACTT TCGGGAGCAG CTGCTGGCAT GTTCTGGAGT AGAAGCGGGG 
CGAGTGGTGG AGTTTTCCTG TGGTCACGTG ATCCCTCCAG ACAACATCTT GCCCCTTATT 
ATATGCAGTG GGCCCTCTAA CCAGCAGCTG GAATTCACCT ACCAGAGAAG AGAGCTGCCT 
CAGATGGTGG AGGAGACAGG CCGCATTCTC TGTAACTTGT GCAATGTGGT CCCTGGAGGT 
GTGGTATGCT TTCTCCCTTC TTATGAGTAC CTGCGCCAGG TACATGCTCA CTGGGACAAG 
ACTGGCCTGC TGACACGTTT GTCTGTCAGG AAAAAGATAT TTCAAGAGCC CAAAAGAGCG 
AGCCAGGTGG AGCAGGTGCT GATGGCGTAT TCCAAGTGCA TTATGAGCTG CAGTCACTCA 
GAAGGCCATC TGACAGGGGC CTTGCTCCTC TCTGTGGTTG GAGGGAAGAT GAGTGAAGGG 
ATCAATTTCT CTGATGACCT GGGCCGGTGT GTGGTGATGG TGGGGATGCC ATATCCCAAC 
ATTAAGTCTC CAGAGCTGCA AGAGAAGATG GCCTACTTGA ATCAGACACT TCCTAGGACA 
CAAGGTCAGC CCCTGCCAGG GACGGTATTG ATAGAGAATC TGTGTATGAA GGCTATAAAC 
CAATCCATAG GCAGGGCCAT TAGGCACCAG AGGGACTTTG CCAGCATTGT GCTCCTGGAT 
CACCGGTATG CGCGTCCTTC TATTCTGGCG AAGCTGCCAG CCTGGATACG AGACCGAGTG 
GAGGTCAAAG CTACCTTTGG GCCTGCATTT GCTGCTGTGC GGAAGTTTCA TCGAGAGAAG 
TCACACCCAA GTCTGGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx11 ( XM_006524408.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524471.1 Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X1, mRNA.

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CloneID	OMu53687
Clone ID Related Accession (Same CDS sequence)	XM_006524408.3 , XM_006524409.3 , NM_001348292.1
Accession Version	XM_006524408.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent DNA helicase DDX11 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524408.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGG TGAGGAGGAG GAAGCGAGAG 
GAGCGACTGA AGGAGGTCTG CCAGGATGGG AGGCTCAGGT TTGCAGCCAA GCGCACGAAA 
CATGAGGAAG AGGAGACTGA GGCTCTCCTG CGCCTCAGCA GGGAGATGCT GGATGCAGGG 
ACAGGACCAG AGCAGCTGGA GCAGCTGGAG TGTGGGGAGG AGCACCTGGT TCTGGCAGAA 
TATGAGAGTG ATGAGGAGAG AAGAGGCAGC AGGGTGGACG AGGCTGAGGA TGACTTGGAG 
GAAGAACATA TAACCAAGAT TTATTACTGC AGTCGGACAC ACTCACAGCT GGCCCAGTTT 
GTGCGGGAAG TACTGAAGAG CCCTTTTGGC AAGGAGACCC GGCTAGTCTC ACTTGGCTCT 
AGGCAGACCC TTTGTGTGAA TGAAGATGTG AAAAACCTGG GTTCTGTGCA ACTTATGAAT 
GACCGCTGTG TGGACATGCA AAGAAGCAAA CGCGAAAAGA ACGGGACTGG GGAAGACAAG 
CCAAAGAGAA AGAGACAGAA GATCCAGACC TCTTGCCCCT TCTACAACCA CGAGCAGATG 
GAGCTTCTCC GGGATGAGAT TCTGCTGGAA GTGAAGGACA TGGAACAACT TGTGGCCCTT 
GGGAAGGAGG CCCGGGCCTG CCCCTATTAC GGAAGCCGCT TTGCCATCCC TGCAGCCCAG 
CTAGTGGTGC TACCCTACCC AATGTTGCTG CATGCAGCTA CCCGACAGGC TGCGGGAATC 
CGGCTACAAG GTCAGGTGGT CATTATTGAC GAGGCTCACA ACCTGATCGA CACCATCACC 
AACATCCACA GCACAGAGGT CAATGGTTCC CAGCTCTGCC AGGCCCATTC CCAGTTGCTC 
CAGTACATGG AAAGATACAG GAAGCGTTTA AAGGCCAAGA ACTTGATGTA CATCAAGCAG 
ATCCTGTACT TGCTGGAGAA GTTTGTGGCT GTTTTGGGAG GTAATGTTAA GCAAAATCCT 
ACTACACAGA GCCTGTCTCA AACAGGGTCT GAGCTGAAGA GCATCAACGA CTTTCTCTTT 
CAGAGCCAGG TGGACAACAT CAACCTCTTC AAGGTGCAGC GGTACTTGGA GAAGAGCATG 
CTCAGCAGGA AGCTCTTTGG CTTCACAGAA TGTTTCGGAG TGGTTCTTCC ATCCCTCTCA 
GACTCTCAGG AAAACCGAGG TCTGGCTGGC TTCCAGCAGT TCCTGAAGAG CCTGCAGTCA 
GGGCCTACTG AGGACTCCCC GGAGGAGGGC CAGGCTGTAG CCCTGCGGCC TGCCTCTCCA 
CTGATGCACA TCGAGGCCTT CCTGGCAGCT CTCACCACAG CCAATCAGGA TGGCAGGGTT 
ATTGTGAACC GCCAAGGCAG TGTGGGTCAG AGCAGCCTTA AATTCTTACT CTTGAACCCA 
GCTGTGCACT TTGCCCAGGT GGTGAAGGAA TGCCGAGCAG TGGTCATTGC AGGTGGCACC 
ATGCAGCCGA TGTCTGACTT TCGGGAGCAG CTGCTGGCAT GTTCTGGAGT AGAAGCGGGG 
CGAGTGGTGG AGTTTTCCTG TGGTCACGTG ATCCCTCCAG ACAACATCTT GCCCCTTATT 
ATATGCAGTG GGCCCTCTAA CCAGCAGCTG GAATTCACCT ACCAGAGAAG AGAGCTGCCT 
CAGATGGTGG AGGAGACAGG CCGCATTCTC TGTAACTTGT GCAATGTGGT CCCTGGAGGT 
GTGGTATGCT TTCTCCCTTC TTATGAGTAC CTGCGCCAGG TACATGCTCA CTGGGACAAG 
ACTGGCCTGC TGACACGTTT GTCTGTCAGG AAAAAGATAT TTCAAGAGCC CAAAAGAGCG 
AGCCAGGTGG AGCAGGTGCT GATGGCGTAT TCCAAGTGCA TTATGAGCTG CAGTCACTCA 
GAAGGCCATC TGACAGGGGC CTTGCTCCTC TCTGTGGTTG GAGGGAAGAT GAGTGAAGGG 
ATCAATTTCT CTGATGACCT GGGCCGGTGT GTGGTGATGG TGGGGATGCC ATATCCCAAC 
ATTAAGTCTC CAGAGCTGCA AGAGAAGATG GCCTACTTGA ATCAGACACT TCCTAGGACA 
CAAGGTCAGC CCCTGCCAGG GACGGTATTG ATAGAGAATC TGTGTATGAA GGCTATAAAC 
CAATCCATAG GCAGGGCCAT TAGGCACCAG AGGGACTTTG CCAGCATTGT GCTCCTGGAT 
CACCGGTATG CGCGTCCTTC TATTCTGGCG AAGCTGCCAG CCTGGATACG AGACCGAGTG 
GAGGTCAAAG CTACCTTTGG GCCTGCATTT GCTGCTGTGC GGAAGTTTCA TCGAGAGAAG 
TCACACCCAA GTCTGGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524471.1
CDS	189..2909
Translation	MADENQEIGGIHFPFPFPPYPIQKDFMAELYKVLEGGKIGIFESPTGTGKSLSLICGALSWLRDFEKKKLQAEALLLAPGSGPPSSEKNSLLTSSSCQEPTDTPRPAGEPDWVTEFVQKKEERDLVERLREEQVRRRKREERLKEVCQDGRLRFAAKRTKHEEEETEALLRLSREMLDAGTGPEQLEQLECGEEHLVLAEYESDEERRGSRVDEAEDDLEEEHITKIYYCSRTHSQLAQFVREVLKSPFGKETRLVSLGSRQTLCVNEDVKNLGSVQLMNDRCVDMQRSKREKNGTGEDKPKRKRQKIQTSCPFYNHEQMELLRDEILLEVKDMEQLVALGKEARACPYYGSRFAIPAAQLVVLPYPMLLHAATRQAAGIRLQGQVVIIDEAHNLIDTITNIHSTEVNGSQLCQAHSQLLQYMERYRKRLKAKNLMYIKQILYLLEKFVAVLGGNVKQNPTTQSLSQTGSELKSINDFLFQSQVDNINLFKVQRYLEKSMLSRKLFGFTECFGVVLPSLSDSQENRGLAGFQQFLKSLQSGPTEDSPEEGQAVALRPASPLMHIEAFLAALTTANQDGRVIVNRQGSVGQSSLKFLLLNPAVHFAQVVKECRAVVIAGGTMQPMSDFREQLLACSGVEAGRVVEFSCGHVIPPDNILPLIICSGPSNQQLEFTYQRRELPQMVEETGRILCNLCNVVPGGVVCFLPSYEYLRQVHAHWDKTGLLTRLSVRKKIFQEPKRASQVEQVLMAYSKCIMSCSHSEGHLTGALLLSVVGGKMSEGINFSDDLGRCVVMVGMPYPNIKSPELQEKMAYLNQTLPRTQGQPLPGTVLIENLCMKAINQSIGRAIRHQRDFASIVLLDHRYARPSILAKLPAWIRDRVEVKATFGPAFAAVRKFHREKSHPSLV

Target ORF information:

RefSeq Version	XM_006524408.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524408.3

ORF Insert Sequence:

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGG TGAGGAGGAG GAAGCGAGAG 
GAGCGACTGA AGGAGGTCTG CCAGGATGGG AGGCTCAGGT TTGCAGCCAA GCGCACGAAA 
CATGAGGAAG AGGAGACTGA GGCTCTCCTG CGCCTCAGCA GGGAGATGCT GGATGCAGGG 
ACAGGACCAG AGCAGCTGGA GCAGCTGGAG TGTGGGGAGG AGCACCTGGT TCTGGCAGAA 
TATGAGAGTG ATGAGGAGAG AAGAGGCAGC AGGGTGGACG AGGCTGAGGA TGACTTGGAG 
GAAGAACATA TAACCAAGAT TTATTACTGC AGTCGGACAC ACTCACAGCT GGCCCAGTTT 
GTGCGGGAAG TACTGAAGAG CCCTTTTGGC AAGGAGACCC GGCTAGTCTC ACTTGGCTCT 
AGGCAGACCC TTTGTGTGAA TGAAGATGTG AAAAACCTGG GTTCTGTGCA ACTTATGAAT 
GACCGCTGTG TGGACATGCA AAGAAGCAAA CGCGAAAAGA ACGGGACTGG GGAAGACAAG 
CCAAAGAGAA AGAGACAGAA GATCCAGACC TCTTGCCCCT TCTACAACCA CGAGCAGATG 
GAGCTTCTCC GGGATGAGAT TCTGCTGGAA GTGAAGGACA TGGAACAACT TGTGGCCCTT 
GGGAAGGAGG CCCGGGCCTG CCCCTATTAC GGAAGCCGCT TTGCCATCCC TGCAGCCCAG 
CTAGTGGTGC TACCCTACCC AATGTTGCTG CATGCAGCTA CCCGACAGGC TGCGGGAATC 
CGGCTACAAG GTCAGGTGGT CATTATTGAC GAGGCTCACA ACCTGATCGA CACCATCACC 
AACATCCACA GCACAGAGGT CAATGGTTCC CAGCTCTGCC AGGCCCATTC CCAGTTGCTC 
CAGTACATGG AAAGATACAG GAAGCGTTTA AAGGCCAAGA ACTTGATGTA CATCAAGCAG 
ATCCTGTACT TGCTGGAGAA GTTTGTGGCT GTTTTGGGAG GTAATGTTAA GCAAAATCCT 
ACTACACAGA GCCTGTCTCA AACAGGGTCT GAGCTGAAGA GCATCAACGA CTTTCTCTTT 
CAGAGCCAGG TGGACAACAT CAACCTCTTC AAGGTGCAGC GGTACTTGGA GAAGAGCATG 
CTCAGCAGGA AGCTCTTTGG CTTCACAGAA TGTTTCGGAG TGGTTCTTCC ATCCCTCTCA 
GACTCTCAGG AAAACCGAGG TCTGGCTGGC TTCCAGCAGT TCCTGAAGAG CCTGCAGTCA 
GGGCCTACTG AGGACTCCCC GGAGGAGGGC CAGGCTGTAG CCCTGCGGCC TGCCTCTCCA 
CTGATGCACA TCGAGGCCTT CCTGGCAGCT CTCACCACAG CCAATCAGGA TGGCAGGGTT 
ATTGTGAACC GCCAAGGCAG TGTGGGTCAG AGCAGCCTTA AATTCTTACT CTTGAACCCA 
GCTGTGCACT TTGCCCAGGT GGTGAAGGAA TGCCGAGCAG TGGTCATTGC AGGTGGCACC 
ATGCAGCCGA TGTCTGACTT TCGGGAGCAG CTGCTGGCAT GTTCTGGAGT AGAAGCGGGG 
CGAGTGGTGG AGTTTTCCTG TGGTCACGTG ATCCCTCCAG ACAACATCTT GCCCCTTATT 
ATATGCAGTG GGCCCTCTAA CCAGCAGCTG GAATTCACCT ACCAGAGAAG AGAGCTGCCT 
CAGATGGTGG AGGAGACAGG CCGCATTCTC TGTAACTTGT GCAATGTGGT CCCTGGAGGT 
GTGGTATGCT TTCTCCCTTC TTATGAGTAC CTGCGCCAGG TACATGCTCA CTGGGACAAG 
ACTGGCCTGC TGACACGTTT GTCTGTCAGG AAAAAGATAT TTCAAGAGCC CAAAAGAGCG 
AGCCAGGTGG AGCAGGTGCT GATGGCGTAT TCCAAGTGCA TTATGAGCTG CAGTCACTCA 
GAAGGCCATC TGACAGGGGC CTTGCTCCTC TCTGTGGTTG GAGGGAAGAT GAGTGAAGGG 
ATCAATTTCT CTGATGACCT GGGCCGGTGT GTGGTGATGG TGGGGATGCC ATATCCCAAC 
ATTAAGTCTC CAGAGCTGCA AGAGAAGATG GCCTACTTGA ATCAGACACT TCCTAGGACA 
CAAGGTCAGC CCCTGCCAGG GACGGTATTG ATAGAGAATC TGTGTATGAA GGCTATAAAC 
CAATCCATAG GCAGGGCCAT TAGGCACCAG AGGGACTTTG CCAGCATTGT GCTCCTGGAT 
CACCGGTATG CGCGTCCTTC TATTCTGGCG AAGCTGCCAG CCTGGATACG AGACCGAGTG 
GAGGTCAAAG CTACCTTTGG GCCTGCATTT GCTGCTGTGC GGAAGTTTCA TCGAGAGAAG 
TCACACCCAA GTCTGGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx11 ( XM_006524409.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524472.1 Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53687
Clone ID Related Accession (Same CDS sequence)	XM_006524408.3 , XM_006524409.3 , NM_001348292.1
Accession Version	XM_006524409.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent DNA helicase DDX11 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524409.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGG TGAGGAGGAG GAAGCGAGAG 
GAGCGACTGA AGGAGGTCTG CCAGGATGGG AGGCTCAGGT TTGCAGCCAA GCGCACGAAA 
CATGAGGAAG AGGAGACTGA GGCTCTCCTG CGCCTCAGCA GGGAGATGCT GGATGCAGGG 
ACAGGACCAG AGCAGCTGGA GCAGCTGGAG TGTGGGGAGG AGCACCTGGT TCTGGCAGAA 
TATGAGAGTG ATGAGGAGAG AAGAGGCAGC AGGGTGGACG AGGCTGAGGA TGACTTGGAG 
GAAGAACATA TAACCAAGAT TTATTACTGC AGTCGGACAC ACTCACAGCT GGCCCAGTTT 
GTGCGGGAAG TACTGAAGAG CCCTTTTGGC AAGGAGACCC GGCTAGTCTC ACTTGGCTCT 
AGGCAGACCC TTTGTGTGAA TGAAGATGTG AAAAACCTGG GTTCTGTGCA ACTTATGAAT 
GACCGCTGTG TGGACATGCA AAGAAGCAAA CGCGAAAAGA ACGGGACTGG GGAAGACAAG 
CCAAAGAGAA AGAGACAGAA GATCCAGACC TCTTGCCCCT TCTACAACCA CGAGCAGATG 
GAGCTTCTCC GGGATGAGAT TCTGCTGGAA GTGAAGGACA TGGAACAACT TGTGGCCCTT 
GGGAAGGAGG CCCGGGCCTG CCCCTATTAC GGAAGCCGCT TTGCCATCCC TGCAGCCCAG 
CTAGTGGTGC TACCCTACCC AATGTTGCTG CATGCAGCTA CCCGACAGGC TGCGGGAATC 
CGGCTACAAG GTCAGGTGGT CATTATTGAC GAGGCTCACA ACCTGATCGA CACCATCACC 
AACATCCACA GCACAGAGGT CAATGGTTCC CAGCTCTGCC AGGCCCATTC CCAGTTGCTC 
CAGTACATGG AAAGATACAG GAAGCGTTTA AAGGCCAAGA ACTTGATGTA CATCAAGCAG 
ATCCTGTACT TGCTGGAGAA GTTTGTGGCT GTTTTGGGAG GTAATGTTAA GCAAAATCCT 
ACTACACAGA GCCTGTCTCA AACAGGGTCT GAGCTGAAGA GCATCAACGA CTTTCTCTTT 
CAGAGCCAGG TGGACAACAT CAACCTCTTC AAGGTGCAGC GGTACTTGGA GAAGAGCATG 
CTCAGCAGGA AGCTCTTTGG CTTCACAGAA TGTTTCGGAG TGGTTCTTCC ATCCCTCTCA 
GACTCTCAGG AAAACCGAGG TCTGGCTGGC TTCCAGCAGT TCCTGAAGAG CCTGCAGTCA 
GGGCCTACTG AGGACTCCCC GGAGGAGGGC CAGGCTGTAG CCCTGCGGCC TGCCTCTCCA 
CTGATGCACA TCGAGGCCTT CCTGGCAGCT CTCACCACAG CCAATCAGGA TGGCAGGGTT 
ATTGTGAACC GCCAAGGCAG TGTGGGTCAG AGCAGCCTTA AATTCTTACT CTTGAACCCA 
GCTGTGCACT TTGCCCAGGT GGTGAAGGAA TGCCGAGCAG TGGTCATTGC AGGTGGCACC 
ATGCAGCCGA TGTCTGACTT TCGGGAGCAG CTGCTGGCAT GTTCTGGAGT AGAAGCGGGG 
CGAGTGGTGG AGTTTTCCTG TGGTCACGTG ATCCCTCCAG ACAACATCTT GCCCCTTATT 
ATATGCAGTG GGCCCTCTAA CCAGCAGCTG GAATTCACCT ACCAGAGAAG AGAGCTGCCT 
CAGATGGTGG AGGAGACAGG CCGCATTCTC TGTAACTTGT GCAATGTGGT CCCTGGAGGT 
GTGGTATGCT TTCTCCCTTC TTATGAGTAC CTGCGCCAGG TACATGCTCA CTGGGACAAG 
ACTGGCCTGC TGACACGTTT GTCTGTCAGG AAAAAGATAT TTCAAGAGCC CAAAAGAGCG 
AGCCAGGTGG AGCAGGTGCT GATGGCGTAT TCCAAGTGCA TTATGAGCTG CAGTCACTCA 
GAAGGCCATC TGACAGGGGC CTTGCTCCTC TCTGTGGTTG GAGGGAAGAT GAGTGAAGGG 
ATCAATTTCT CTGATGACCT GGGCCGGTGT GTGGTGATGG TGGGGATGCC ATATCCCAAC 
ATTAAGTCTC CAGAGCTGCA AGAGAAGATG GCCTACTTGA ATCAGACACT TCCTAGGACA 
CAAGGTCAGC CCCTGCCAGG GACGGTATTG ATAGAGAATC TGTGTATGAA GGCTATAAAC 
CAATCCATAG GCAGGGCCAT TAGGCACCAG AGGGACTTTG CCAGCATTGT GCTCCTGGAT 
CACCGGTATG CGCGTCCTTC TATTCTGGCG AAGCTGCCAG CCTGGATACG AGACCGAGTG 
GAGGTCAAAG CTACCTTTGG GCCTGCATTT GCTGCTGTGC GGAAGTTTCA TCGAGAGAAG 
TCACACCCAA GTCTGGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524472.1
CDS	122..2842
Translation	MADENQEIGGIHFPFPFPPYPIQKDFMAELYKVLEGGKIGIFESPTGTGKSLSLICGALSWLRDFEKKKLQAEALLLAPGSGPPSSEKNSLLTSSSCQEPTDTPRPAGEPDWVTEFVQKKEERDLVERLREEQVRRRKREERLKEVCQDGRLRFAAKRTKHEEEETEALLRLSREMLDAGTGPEQLEQLECGEEHLVLAEYESDEERRGSRVDEAEDDLEEEHITKIYYCSRTHSQLAQFVREVLKSPFGKETRLVSLGSRQTLCVNEDVKNLGSVQLMNDRCVDMQRSKREKNGTGEDKPKRKRQKIQTSCPFYNHEQMELLRDEILLEVKDMEQLVALGKEARACPYYGSRFAIPAAQLVVLPYPMLLHAATRQAAGIRLQGQVVIIDEAHNLIDTITNIHSTEVNGSQLCQAHSQLLQYMERYRKRLKAKNLMYIKQILYLLEKFVAVLGGNVKQNPTTQSLSQTGSELKSINDFLFQSQVDNINLFKVQRYLEKSMLSRKLFGFTECFGVVLPSLSDSQENRGLAGFQQFLKSLQSGPTEDSPEEGQAVALRPASPLMHIEAFLAALTTANQDGRVIVNRQGSVGQSSLKFLLLNPAVHFAQVVKECRAVVIAGGTMQPMSDFREQLLACSGVEAGRVVEFSCGHVIPPDNILPLIICSGPSNQQLEFTYQRRELPQMVEETGRILCNLCNVVPGGVVCFLPSYEYLRQVHAHWDKTGLLTRLSVRKKIFQEPKRASQVEQVLMAYSKCIMSCSHSEGHLTGALLLSVVGGKMSEGINFSDDLGRCVVMVGMPYPNIKSPELQEKMAYLNQTLPRTQGQPLPGTVLIENLCMKAINQSIGRAIRHQRDFASIVLLDHRYARPSILAKLPAWIRDRVEVKATFGPAFAAVRKFHREKSHPSLV

Target ORF information:

RefSeq Version	XM_006524409.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524409.3

ORF Insert Sequence:

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGG TGAGGAGGAG GAAGCGAGAG 
GAGCGACTGA AGGAGGTCTG CCAGGATGGG AGGCTCAGGT TTGCAGCCAA GCGCACGAAA 
CATGAGGAAG AGGAGACTGA GGCTCTCCTG CGCCTCAGCA GGGAGATGCT GGATGCAGGG 
ACAGGACCAG AGCAGCTGGA GCAGCTGGAG TGTGGGGAGG AGCACCTGGT TCTGGCAGAA 
TATGAGAGTG ATGAGGAGAG AAGAGGCAGC AGGGTGGACG AGGCTGAGGA TGACTTGGAG 
GAAGAACATA TAACCAAGAT TTATTACTGC AGTCGGACAC ACTCACAGCT GGCCCAGTTT 
GTGCGGGAAG TACTGAAGAG CCCTTTTGGC AAGGAGACCC GGCTAGTCTC ACTTGGCTCT 
AGGCAGACCC TTTGTGTGAA TGAAGATGTG AAAAACCTGG GTTCTGTGCA ACTTATGAAT 
GACCGCTGTG TGGACATGCA AAGAAGCAAA CGCGAAAAGA ACGGGACTGG GGAAGACAAG 
CCAAAGAGAA AGAGACAGAA GATCCAGACC TCTTGCCCCT TCTACAACCA CGAGCAGATG 
GAGCTTCTCC GGGATGAGAT TCTGCTGGAA GTGAAGGACA TGGAACAACT TGTGGCCCTT 
GGGAAGGAGG CCCGGGCCTG CCCCTATTAC GGAAGCCGCT TTGCCATCCC TGCAGCCCAG 
CTAGTGGTGC TACCCTACCC AATGTTGCTG CATGCAGCTA CCCGACAGGC TGCGGGAATC 
CGGCTACAAG GTCAGGTGGT CATTATTGAC GAGGCTCACA ACCTGATCGA CACCATCACC 
AACATCCACA GCACAGAGGT CAATGGTTCC CAGCTCTGCC AGGCCCATTC CCAGTTGCTC 
CAGTACATGG AAAGATACAG GAAGCGTTTA AAGGCCAAGA ACTTGATGTA CATCAAGCAG 
ATCCTGTACT TGCTGGAGAA GTTTGTGGCT GTTTTGGGAG GTAATGTTAA GCAAAATCCT 
ACTACACAGA GCCTGTCTCA AACAGGGTCT GAGCTGAAGA GCATCAACGA CTTTCTCTTT 
CAGAGCCAGG TGGACAACAT CAACCTCTTC AAGGTGCAGC GGTACTTGGA GAAGAGCATG 
CTCAGCAGGA AGCTCTTTGG CTTCACAGAA TGTTTCGGAG TGGTTCTTCC ATCCCTCTCA 
GACTCTCAGG AAAACCGAGG TCTGGCTGGC TTCCAGCAGT TCCTGAAGAG CCTGCAGTCA 
GGGCCTACTG AGGACTCCCC GGAGGAGGGC CAGGCTGTAG CCCTGCGGCC TGCCTCTCCA 
CTGATGCACA TCGAGGCCTT CCTGGCAGCT CTCACCACAG CCAATCAGGA TGGCAGGGTT 
ATTGTGAACC GCCAAGGCAG TGTGGGTCAG AGCAGCCTTA AATTCTTACT CTTGAACCCA 
GCTGTGCACT TTGCCCAGGT GGTGAAGGAA TGCCGAGCAG TGGTCATTGC AGGTGGCACC 
ATGCAGCCGA TGTCTGACTT TCGGGAGCAG CTGCTGGCAT GTTCTGGAGT AGAAGCGGGG 
CGAGTGGTGG AGTTTTCCTG TGGTCACGTG ATCCCTCCAG ACAACATCTT GCCCCTTATT 
ATATGCAGTG GGCCCTCTAA CCAGCAGCTG GAATTCACCT ACCAGAGAAG AGAGCTGCCT 
CAGATGGTGG AGGAGACAGG CCGCATTCTC TGTAACTTGT GCAATGTGGT CCCTGGAGGT 
GTGGTATGCT TTCTCCCTTC TTATGAGTAC CTGCGCCAGG TACATGCTCA CTGGGACAAG 
ACTGGCCTGC TGACACGTTT GTCTGTCAGG AAAAAGATAT TTCAAGAGCC CAAAAGAGCG 
AGCCAGGTGG AGCAGGTGCT GATGGCGTAT TCCAAGTGCA TTATGAGCTG CAGTCACTCA 
GAAGGCCATC TGACAGGGGC CTTGCTCCTC TCTGTGGTTG GAGGGAAGAT GAGTGAAGGG 
ATCAATTTCT CTGATGACCT GGGCCGGTGT GTGGTGATGG TGGGGATGCC ATATCCCAAC 
ATTAAGTCTC CAGAGCTGCA AGAGAAGATG GCCTACTTGA ATCAGACACT TCCTAGGACA 
CAAGGTCAGC CCCTGCCAGG GACGGTATTG ATAGAGAATC TGTGTATGAA GGCTATAAAC 
CAATCCATAG GCAGGGCCAT TAGGCACCAG AGGGACTTTG CCAGCATTGT GCTCCTGGAT 
CACCGGTATG CGCGTCCTTC TATTCTGGCG AAGCTGCCAG CCTGGATACG AGACCGAGTG 
GAGGTCAAAG CTACCTTTGG GCCTGCATTT GCTGCTGTGC GGAAGTTTCA TCGAGAGAAG 
TCACACCCAA GTCTGGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx11 ( NM_001003919.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001003919.1 Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), mRNA.

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CloneID	OMu10542
Clone ID Related Accession (Same CDS sequence)	NM_001003919.1 , NM_001003919.2
Accession Version	NM_001003919.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	probable ATP-dependent RNA helicase DDX11
Comment

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGA AACATGAGGA AGAGGAGACT 
GAGGCTCTCC TGCGCCTCAG CAGGGAGATG CTGGATGCAG GGACAGGACC AGAGCAGCTG 
GAGCAGCTGG AGTGTGGGGA GGAGCACCTG GTTCTGGCAG AATATGAGAG TGATGAGGAG 
AGAAGAGGCA GCAGGGTGGA CGAGGCTGAG GATGACTTGG AGGAAGAACA TATAACCAAG 
ATTTATTACT GCAGTCGGAC ACACTCACAG CTGGCCCAGT TTGTGCGGGA AGTACTGAAG 
AGCCCTTTTG GCAAGGAGAC CCGGCTAGTC TCACTTGGCT CTAGGCAGAC CCTTTGTGTG 
AATGAAGATG TGAAAAACCT GGGTTCTGTG CAACTTATGA ATGACCGCTG TGTGGACATG 
CAAAGAAGCA AACGCGAAAA GAACGGGACT GGGGAAGACA AGCCAAAGAG AAAGAGACAG 
AAGATCCAGA CCTCTTGCCC CTTCTACAAC CACGAGCAGA TGGAGCTTCT CCGGGATGAG 
ATTCTGCTGG AAGTGAAGGA CATGGAACAA CTTGTGGCCC TTGGGAAGGA GGCCCGGGCC 
TGCCCCTATT ACGGAAGCCG CTTTGCCATC CCTGCAGCCC AGCTAGTGGT GCTACCCTAC 
CCAATGTTGC TGCATGCAGC TACCCGACAG GCTGCGGGAA TCCGGCTACA AGGTCAGGTG 
GTCATTATTG ACGAGGCTCA CAACCTGATC GACACCATCA CCAACATCCA CAGCACAGAG 
GTCAATGGTT CCCAGCTCTG CCAGGCCCAT TCCCAGTTGC TCCAGTACAT GGAAAGATAC 
AGGAAGCGTT TAAAGGCCAA GAACTTGATG TACATCAAGC AGATCCTGTA CTTGCTGGAG 
AAGTTTGTGG CTGTTTTGGG AGGTAATGTT AAGCAAAATC CTACTACACA GAGCCTGTCT 
CAAACAGGGT CTGAGCTGAA GAGCATCAAC GACTTTCTCT TTCAGAGCCA GGTGGACAAC 
ATCAACCTCT TCAAGGTGCA GCGGTACTTG GAGAAGAGCA TGCTCAGCAG GAAGCTCTTT 
GGCTTCACAG AATGTTTCGG AGTGGTTCTT CCATCCCTCT CAGACTCTCA GGAAAACCGA 
GGTCTGGCTG GCTTCCAGCA GTTCCTGAAG AGCCTGCAGT CAGGGCCTAC TGAGGACTCC 
CCGGAGGAGG GCCAGGCTGT AGCCCTGCGG CCTGCCTCTC CACTGATGCA CATCGAGGCC 
TTCCTGGCAG CTCTCACCAC AGCCAATCAG GATGGCAGGG TTATTGTGAA CCGCCAAGGC 
AGTGTGGGTC AGAGCAGCCT TAAATTCTTA CTCTTGAACC CAGCTGTGCA CTTTGCCCAG 
GTGGTGAAGG AATGCCGAGC AGTGGTCATT GCAGGTGGCA CCATGCAGCC GATGTCTGAC 
TTTCGGGAGC AGCTGCTGGC ATGTTCTGGA GTAGAAGCGG GGCGAGTGGT GGAGTTTTCC 
TGTGGTCACG TGATCCCTCC AGACAACATC TTGCCCCTTA TTATATGCAG TGGGCCCTCT 
AACCAGCAGC TGGAATTCAC CTACCAGAGA AGAGAGCTGC CTCAGATGGT GGAGGAGACA 
GGCCGCATTC TCTGTAACTT GTGCAATGTG GTCCCTGGAG GTGTGGTATG CTTTCTCCCT 
TCTTATGAGT ACCTGCGCCA GGTACATGCT CACTGGGACA AGACTGGCCT GCTGACACGT 
TTGTCTGTCA GGAAAAAGAT ATTTCAAGAG CCCAAAAGAG CGAGCCAGGT GGAGCAGGTG 
CTGATGGCGT ATTCCAAGTG CATTATGAGC TGCAGTCACT CAGAAGGCCA TCTGACAGGG 
GCCTTGCTCC TCTCTGTGGT TGGAGGGAAG ATGAGTGAAG GGATCAATTT CTCTGATGAC 
CTGGGCCGGT GTGTGGTGAT GGTGGGGATG CCATATCCCA ACATTAAGTC TCCAGAGCTG 
CAAGAGAAGA TGGCCTACTT GAATCAGACA CTTCCTAGGA CACAAGGTCA GCCCCTGCCA 
GGGACGGTAT TGATAGAGAA TCTGTGTATG AAGGCTATAA ACCAATCCAT AGGCAGGGCC 
ATTAGGCACC AGAGGGACTT TGCCAGCATT GTGCTCCTGG ATCACCGGTA TGCGCGTCCT 
TCTATTCTGG CGAAGCTGCC AGCCTGGATA CGAGACCGAG TGGAGGTCAA AGCTACCTTT 
GGGCCTGCAT TTGCTGCTGT GCGGAAGTTT CATCGAGAGA AGTCACACCC AAGTCTGGTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_001003919.1

Translation

MADENQEIGGIHFPFPFPPYPIQKDFMAELYKVLEGGKIGIFESPTGTGKSLSLICGALSWLRDFEKKKLQAEALLLAPGSGPPSSEKNSLLTSSSCQEPTDTPRPAGEPDWVTEFVQKKEERDLVERLREEQKHEEEETEALLRLSREMLDAGTGPEQLEQLECGEEHLVLAEYESDEERRGSRVDEAEDDLEEEHITKIYYCSRTHSQLAQFVREVLKSPFGKETRLVSLGSRQTLCVNEDVKNLGSVQLMNDRCVDMQRSKREKNGTGEDKPKRKRQKIQTSCPFYNHEQMELLRDEILLEVKDMEQLVALGKEARACPYYGSRFAIPAAQLVVLPYPMLLHAATRQAAGIRLQGQVVIIDEAHNLIDTITNIHSTEVNGSQLCQAHSQLLQYMERYRKRLKAKNLMYIKQILYLLEKFVAVLGGNVKQNPTTQSLSQTGSELKSINDFLFQSQVDNINLFKVQRYLEKSMLSRKLFGFTECFGVVLPSLSDSQENRGLAGFQQFLKSLQSGPTEDSPEEGQAVALRPASPLMHIEAFLAALTTANQDGRVIVNRQGSVGQSSLKFLLLNPAVHFAQVVKECRAVVIAGGTMQPMSDFREQLLACSGVEAGRVVEFSCGHVIPPDNILPLIICSGPSNQQLEFTYQRRELPQMVEETGRILCNLCNVVPGGVVCFLPSYEYLRQVHAHWDKTGLLTRLSVRKKIFQEPKRASQVEQVLMAYSKCIMSCSHSEGHLTGALLLSVVGGKMSEGINFSDDLGRCVVMVGMPYPNIKSPELQEKMAYLNQTLPRTQGQPLPGTVLIENLCMKAINQSIGRAIRHQRDFASIVLLDHRYARPSILAKLPAWIRDRVEVKATFGPAFAAVRKFHREKSHPSLV

Target ORF information:

RefSeq Version	NM_001003919.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003919.1

ORF Insert Sequence:

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGA AACATGAGGA AGAGGAGACT 
GAGGCTCTCC TGCGCCTCAG CAGGGAGATG CTGGATGCAG GGACAGGACC AGAGCAGCTG 
GAGCAGCTGG AGTGTGGGGA GGAGCACCTG GTTCTGGCAG AATATGAGAG TGATGAGGAG 
AGAAGAGGCA GCAGGGTGGA CGAGGCTGAG GATGACTTGG AGGAAGAACA TATAACCAAG 
ATTTATTACT GCAGTCGGAC ACACTCACAG CTGGCCCAGT TTGTGCGGGA AGTACTGAAG 
AGCCCTTTTG GCAAGGAGAC CCGGCTAGTC TCACTTGGCT CTAGGCAGAC CCTTTGTGTG 
AATGAAGATG TGAAAAACCT GGGTTCTGTG CAACTTATGA ATGACCGCTG TGTGGACATG 
CAAAGAAGCA AACGCGAAAA GAACGGGACT GGGGAAGACA AGCCAAAGAG AAAGAGACAG 
AAGATCCAGA CCTCTTGCCC CTTCTACAAC CACGAGCAGA TGGAGCTTCT CCGGGATGAG 
ATTCTGCTGG AAGTGAAGGA CATGGAACAA CTTGTGGCCC TTGGGAAGGA GGCCCGGGCC 
TGCCCCTATT ACGGAAGCCG CTTTGCCATC CCTGCAGCCC AGCTAGTGGT GCTACCCTAC 
CCAATGTTGC TGCATGCAGC TACCCGACAG GCTGCGGGAA TCCGGCTACA AGGTCAGGTG 
GTCATTATTG ACGAGGCTCA CAACCTGATC GACACCATCA CCAACATCCA CAGCACAGAG 
GTCAATGGTT CCCAGCTCTG CCAGGCCCAT TCCCAGTTGC TCCAGTACAT GGAAAGATAC 
AGGAAGCGTT TAAAGGCCAA GAACTTGATG TACATCAAGC AGATCCTGTA CTTGCTGGAG 
AAGTTTGTGG CTGTTTTGGG AGGTAATGTT AAGCAAAATC CTACTACACA GAGCCTGTCT 
CAAACAGGGT CTGAGCTGAA GAGCATCAAC GACTTTCTCT TTCAGAGCCA GGTGGACAAC 
ATCAACCTCT TCAAGGTGCA GCGGTACTTG GAGAAGAGCA TGCTCAGCAG GAAGCTCTTT 
GGCTTCACAG AATGTTTCGG AGTGGTTCTT CCATCCCTCT CAGACTCTCA GGAAAACCGA 
GGTCTGGCTG GCTTCCAGCA GTTCCTGAAG AGCCTGCAGT CAGGGCCTAC TGAGGACTCC 
CCGGAGGAGG GCCAGGCTGT AGCCCTGCGG CCTGCCTCTC CACTGATGCA CATCGAGGCC 
TTCCTGGCAG CTCTCACCAC AGCCAATCAG GATGGCAGGG TTATTGTGAA CCGCCAAGGC 
AGTGTGGGTC AGAGCAGCCT TAAATTCTTA CTCTTGAACC CAGCTGTGCA CTTTGCCCAG 
GTGGTGAAGG AATGCCGAGC AGTGGTCATT GCAGGTGGCA CCATGCAGCC GATGTCTGAC 
TTTCGGGAGC AGCTGCTGGC ATGTTCTGGA GTAGAAGCGG GGCGAGTGGT GGAGTTTTCC 
TGTGGTCACG TGATCCCTCC AGACAACATC TTGCCCCTTA TTATATGCAG TGGGCCCTCT 
AACCAGCAGC TGGAATTCAC CTACCAGAGA AGAGAGCTGC CTCAGATGGT GGAGGAGACA 
GGCCGCATTC TCTGTAACTT GTGCAATGTG GTCCCTGGAG GTGTGGTATG CTTTCTCCCT 
TCTTATGAGT ACCTGCGCCA GGTACATGCT CACTGGGACA AGACTGGCCT GCTGACACGT 
TTGTCTGTCA GGAAAAAGAT ATTTCAAGAG CCCAAAAGAG CGAGCCAGGT GGAGCAGGTG 
CTGATGGCGT ATTCCAAGTG CATTATGAGC TGCAGTCACT CAGAAGGCCA TCTGACAGGG 
GCCTTGCTCC TCTCTGTGGT TGGAGGGAAG ATGAGTGAAG GGATCAATTT CTCTGATGAC 
CTGGGCCGGT GTGTGGTGAT GGTGGGGATG CCATATCCCA ACATTAAGTC TCCAGAGCTG 
CAAGAGAAGA TGGCCTACTT GAATCAGACA CTTCCTAGGA CACAAGGTCA GCCCCTGCCA 
GGGACGGTAT TGATAGAGAA TCTGTGTATG AAGGCTATAA ACCAATCCAT AGGCAGGGCC 
ATTAGGCACC AGAGGGACTT TGCCAGCATT GTGCTCCTGG ATCACCGGTA TGCGCGTCCT 
TCTATTCTGG CGAAGCTGCC AGCCTGGATA CGAGACCGAG TGGAGGTCAA AGCTACCTTT 
GGGCCTGCAT TTGCTGCTGT GCGGAAGTTT CATCGAGAGA AGTCACACCC AAGTCTGGTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx11 ( NM_001003919.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001003919.1 Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant 1, mRNA.

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CloneID	OMu10542
Clone ID Related Accession (Same CDS sequence)	NM_001003919.1 , NM_001003919.2
Accession Version	NM_001003919.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	ATP-dependent DNA helicase DDX11 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC079656.1 and CT025671.10. On Feb 8, 2017 this sequence version replaced NM_001003919.1. Transcript Variant: This variant (1) uses an alternate in-frame splice site in the 5' coding region compared to variant 2. The encoded isoform (1) is shorter than isoform 2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGA AACATGAGGA AGAGGAGACT 
GAGGCTCTCC TGCGCCTCAG CAGGGAGATG CTGGATGCAG GGACAGGACC AGAGCAGCTG 
GAGCAGCTGG AGTGTGGGGA GGAGCACCTG GTTCTGGCAG AATATGAGAG TGATGAGGAG 
AGAAGAGGCA GCAGGGTGGA CGAGGCTGAG GATGACTTGG AGGAAGAACA TATAACCAAG 
ATTTATTACT GCAGTCGGAC ACACTCACAG CTGGCCCAGT TTGTGCGGGA AGTACTGAAG 
AGCCCTTTTG GCAAGGAGAC CCGGCTAGTC TCACTTGGCT CTAGGCAGAC CCTTTGTGTG 
AATGAAGATG TGAAAAACCT GGGTTCTGTG CAACTTATGA ATGACCGCTG TGTGGACATG 
CAAAGAAGCA AACGCGAAAA GAACGGGACT GGGGAAGACA AGCCAAAGAG AAAGAGACAG 
AAGATCCAGA CCTCTTGCCC CTTCTACAAC CACGAGCAGA TGGAGCTTCT CCGGGATGAG 
ATTCTGCTGG AAGTGAAGGA CATGGAACAA CTTGTGGCCC TTGGGAAGGA GGCCCGGGCC 
TGCCCCTATT ACGGAAGCCG CTTTGCCATC CCTGCAGCCC AGCTAGTGGT GCTACCCTAC 
CCAATGTTGC TGCATGCAGC TACCCGACAG GCTGCGGGAA TCCGGCTACA AGGTCAGGTG 
GTCATTATTG ACGAGGCTCA CAACCTGATC GACACCATCA CCAACATCCA CAGCACAGAG 
GTCAATGGTT CCCAGCTCTG CCAGGCCCAT TCCCAGTTGC TCCAGTACAT GGAAAGATAC 
AGGAAGCGTT TAAAGGCCAA GAACTTGATG TACATCAAGC AGATCCTGTA CTTGCTGGAG 
AAGTTTGTGG CTGTTTTGGG AGGTAATGTT AAGCAAAATC CTACTACACA GAGCCTGTCT 
CAAACAGGGT CTGAGCTGAA GAGCATCAAC GACTTTCTCT TTCAGAGCCA GGTGGACAAC 
ATCAACCTCT TCAAGGTGCA GCGGTACTTG GAGAAGAGCA TGCTCAGCAG GAAGCTCTTT 
GGCTTCACAG AATGTTTCGG AGTGGTTCTT CCATCCCTCT CAGACTCTCA GGAAAACCGA 
GGTCTGGCTG GCTTCCAGCA GTTCCTGAAG AGCCTGCAGT CAGGGCCTAC TGAGGACTCC 
CCGGAGGAGG GCCAGGCTGT AGCCCTGCGG CCTGCCTCTC CACTGATGCA CATCGAGGCC 
TTCCTGGCAG CTCTCACCAC AGCCAATCAG GATGGCAGGG TTATTGTGAA CCGCCAAGGC 
AGTGTGGGTC AGAGCAGCCT TAAATTCTTA CTCTTGAACC CAGCTGTGCA CTTTGCCCAG 
GTGGTGAAGG AATGCCGAGC AGTGGTCATT GCAGGTGGCA CCATGCAGCC GATGTCTGAC 
TTTCGGGAGC AGCTGCTGGC ATGTTCTGGA GTAGAAGCGG GGCGAGTGGT GGAGTTTTCC 
TGTGGTCACG TGATCCCTCC AGACAACATC TTGCCCCTTA TTATATGCAG TGGGCCCTCT 
AACCAGCAGC TGGAATTCAC CTACCAGAGA AGAGAGCTGC CTCAGATGGT GGAGGAGACA 
GGCCGCATTC TCTGTAACTT GTGCAATGTG GTCCCTGGAG GTGTGGTATG CTTTCTCCCT 
TCTTATGAGT ACCTGCGCCA GGTACATGCT CACTGGGACA AGACTGGCCT GCTGACACGT 
TTGTCTGTCA GGAAAAAGAT ATTTCAAGAG CCCAAAAGAG CGAGCCAGGT GGAGCAGGTG 
CTGATGGCGT ATTCCAAGTG CATTATGAGC TGCAGTCACT CAGAAGGCCA TCTGACAGGG 
GCCTTGCTCC TCTCTGTGGT TGGAGGGAAG ATGAGTGAAG GGATCAATTT CTCTGATGAC 
CTGGGCCGGT GTGTGGTGAT GGTGGGGATG CCATATCCCA ACATTAAGTC TCCAGAGCTG 
CAAGAGAAGA TGGCCTACTT GAATCAGACA CTTCCTAGGA CACAAGGTCA GCCCCTGCCA 
GGGACGGTAT TGATAGAGAA TCTGTGTATG AAGGCTATAA ACCAATCCAT AGGCAGGGCC 
ATTAGGCACC AGAGGGACTT TGCCAGCATT GTGCTCCTGG ATCACCGGTA TGCGCGTCCT 
TCTATTCTGG CGAAGCTGCC AGCCTGGATA CGAGACCGAG TGGAGGTCAA AGCTACCTTT 
GGGCCTGCAT TTGCTGCTGT GCGGAAGTTT CATCGAGAGA AGTCACACCC AAGTCTGGTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003919.1
CDS	178..2820
Translation	MADENQEIGGIHFPFPFPPYPIQKDFMAELYKVLEGGKIGIFESPTGTGKSLSLICGALSWLRDFEKKKLQAEALLLAPGSGPPSSEKNSLLTSSSCQEPTDTPRPAGEPDWVTEFVQKKEERDLVERLREEQKHEEEETEALLRLSREMLDAGTGPEQLEQLECGEEHLVLAEYESDEERRGSRVDEAEDDLEEEHITKIYYCSRTHSQLAQFVREVLKSPFGKETRLVSLGSRQTLCVNEDVKNLGSVQLMNDRCVDMQRSKREKNGTGEDKPKRKRQKIQTSCPFYNHEQMELLRDEILLEVKDMEQLVALGKEARACPYYGSRFAIPAAQLVVLPYPMLLHAATRQAAGIRLQGQVVIIDEAHNLIDTITNIHSTEVNGSQLCQAHSQLLQYMERYRKRLKAKNLMYIKQILYLLEKFVAVLGGNVKQNPTTQSLSQTGSELKSINDFLFQSQVDNINLFKVQRYLEKSMLSRKLFGFTECFGVVLPSLSDSQENRGLAGFQQFLKSLQSGPTEDSPEEGQAVALRPASPLMHIEAFLAALTTANQDGRVIVNRQGSVGQSSLKFLLLNPAVHFAQVVKECRAVVIAGGTMQPMSDFREQLLACSGVEAGRVVEFSCGHVIPPDNILPLIICSGPSNQQLEFTYQRRELPQMVEETGRILCNLCNVVPGGVVCFLPSYEYLRQVHAHWDKTGLLTRLSVRKKIFQEPKRASQVEQVLMAYSKCIMSCSHSEGHLTGALLLSVVGGKMSEGINFSDDLGRCVVMVGMPYPNIKSPELQEKMAYLNQTLPRTQGQPLPGTVLIENLCMKAINQSIGRAIRHQRDFASIVLLDHRYARPSILAKLPAWIRDRVEVKATFGPAFAAVRKFHREKSHPSLV

Target ORF information:

RefSeq Version	NM_001003919.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003919.2

ORF Insert Sequence:

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGA AACATGAGGA AGAGGAGACT 
GAGGCTCTCC TGCGCCTCAG CAGGGAGATG CTGGATGCAG GGACAGGACC AGAGCAGCTG 
GAGCAGCTGG AGTGTGGGGA GGAGCACCTG GTTCTGGCAG AATATGAGAG TGATGAGGAG 
AGAAGAGGCA GCAGGGTGGA CGAGGCTGAG GATGACTTGG AGGAAGAACA TATAACCAAG 
ATTTATTACT GCAGTCGGAC ACACTCACAG CTGGCCCAGT TTGTGCGGGA AGTACTGAAG 
AGCCCTTTTG GCAAGGAGAC CCGGCTAGTC TCACTTGGCT CTAGGCAGAC CCTTTGTGTG 
AATGAAGATG TGAAAAACCT GGGTTCTGTG CAACTTATGA ATGACCGCTG TGTGGACATG 
CAAAGAAGCA AACGCGAAAA GAACGGGACT GGGGAAGACA AGCCAAAGAG AAAGAGACAG 
AAGATCCAGA CCTCTTGCCC CTTCTACAAC CACGAGCAGA TGGAGCTTCT CCGGGATGAG 
ATTCTGCTGG AAGTGAAGGA CATGGAACAA CTTGTGGCCC TTGGGAAGGA GGCCCGGGCC 
TGCCCCTATT ACGGAAGCCG CTTTGCCATC CCTGCAGCCC AGCTAGTGGT GCTACCCTAC 
CCAATGTTGC TGCATGCAGC TACCCGACAG GCTGCGGGAA TCCGGCTACA AGGTCAGGTG 
GTCATTATTG ACGAGGCTCA CAACCTGATC GACACCATCA CCAACATCCA CAGCACAGAG 
GTCAATGGTT CCCAGCTCTG CCAGGCCCAT TCCCAGTTGC TCCAGTACAT GGAAAGATAC 
AGGAAGCGTT TAAAGGCCAA GAACTTGATG TACATCAAGC AGATCCTGTA CTTGCTGGAG 
AAGTTTGTGG CTGTTTTGGG AGGTAATGTT AAGCAAAATC CTACTACACA GAGCCTGTCT 
CAAACAGGGT CTGAGCTGAA GAGCATCAAC GACTTTCTCT TTCAGAGCCA GGTGGACAAC 
ATCAACCTCT TCAAGGTGCA GCGGTACTTG GAGAAGAGCA TGCTCAGCAG GAAGCTCTTT 
GGCTTCACAG AATGTTTCGG AGTGGTTCTT CCATCCCTCT CAGACTCTCA GGAAAACCGA 
GGTCTGGCTG GCTTCCAGCA GTTCCTGAAG AGCCTGCAGT CAGGGCCTAC TGAGGACTCC 
CCGGAGGAGG GCCAGGCTGT AGCCCTGCGG CCTGCCTCTC CACTGATGCA CATCGAGGCC 
TTCCTGGCAG CTCTCACCAC AGCCAATCAG GATGGCAGGG TTATTGTGAA CCGCCAAGGC 
AGTGTGGGTC AGAGCAGCCT TAAATTCTTA CTCTTGAACC CAGCTGTGCA CTTTGCCCAG 
GTGGTGAAGG AATGCCGAGC AGTGGTCATT GCAGGTGGCA CCATGCAGCC GATGTCTGAC 
TTTCGGGAGC AGCTGCTGGC ATGTTCTGGA GTAGAAGCGG GGCGAGTGGT GGAGTTTTCC 
TGTGGTCACG TGATCCCTCC AGACAACATC TTGCCCCTTA TTATATGCAG TGGGCCCTCT 
AACCAGCAGC TGGAATTCAC CTACCAGAGA AGAGAGCTGC CTCAGATGGT GGAGGAGACA 
GGCCGCATTC TCTGTAACTT GTGCAATGTG GTCCCTGGAG GTGTGGTATG CTTTCTCCCT 
TCTTATGAGT ACCTGCGCCA GGTACATGCT CACTGGGACA AGACTGGCCT GCTGACACGT 
TTGTCTGTCA GGAAAAAGAT ATTTCAAGAG CCCAAAAGAG CGAGCCAGGT GGAGCAGGTG 
CTGATGGCGT ATTCCAAGTG CATTATGAGC TGCAGTCACT CAGAAGGCCA TCTGACAGGG 
GCCTTGCTCC TCTCTGTGGT TGGAGGGAAG ATGAGTGAAG GGATCAATTT CTCTGATGAC 
CTGGGCCGGT GTGTGGTGAT GGTGGGGATG CCATATCCCA ACATTAAGTC TCCAGAGCTG 
CAAGAGAAGA TGGCCTACTT GAATCAGACA CTTCCTAGGA CACAAGGTCA GCCCCTGCCA 
GGGACGGTAT TGATAGAGAA TCTGTGTATG AAGGCTATAA ACCAATCCAT AGGCAGGGCC 
ATTAGGCACC AGAGGGACTT TGCCAGCATT GTGCTCCTGG ATCACCGGTA TGCGCGTCCT 
TCTATTCTGG CGAAGCTGCC AGCCTGGATA CGAGACCGAG TGGAGGTCAA AGCTACCTTT 
GGGCCTGCAT TTGCTGCTGT GCGGAAGTTT CATCGAGAGA AGTCACACCC AAGTCTGGTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx11 ( XM_006524410.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524473.1 Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X3, mRNA.

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CloneID	OMu53688
Clone ID Related Accession (Same CDS sequence)	XM_006524410.4 , XM_006524410.3
Accession Version	XM_006524410.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent DNA helicase DDX11 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524410.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGG TGAGGAGGAG GAAGCGAGAG 
GAGCGACTGA AGGAGGTCTG CCAGGATGGG AGGCTCAGGT TTGCAGCCAA GCGCACGAAA 
CATGAGGAAG AGGAGACTGA GGCTCTCCTG CGCCTCAGCA GGGAGATGCT GGATGCAGGG 
ACAGGACCAG AGCAGCTGGA GCAGCTGGAG TGTGGGGAGG AGCACCTGGT TCTGGCAGAA 
TATGAGAGTG ATGAGGAGAG AAGAGGCAGC AGGGTGGACG AGGCTGAGGA TGACTTGGAG 
GAAGAACATA TAACCAAGAT TTATTACTGC AGTCGGACAC ACTCACAGCT GGCCCAGTTT 
GTGCGGGAAG TACTGAAGAG CCCTTTTGGC AAGGAGACCC GGCTAGTCTC ACTTGGCTCT 
AGGCAGACCC TTTGTGTGAA TGAAGATGTG AAAAACCTGG GTTCTGTGCA ACTTATGAAT 
GACCGCTGTG TGGACATGCA AAGAAGCAAA CGCGAAAAGA ACGGGACTGG GGAAGACAAG 
CCAAAGAGAA AGAGACAGAA GATCCAGACC TCTTGCCCCT TCTACAACCA CGAGCAGATG 
GAGCTTCTCC GGGATGAGAT TCTGCTGGAA GTGAAGGACA TGGAACAACT TGTGGCCCTT 
GGGAAGGAGG CCCGGGCCTG CCCCTATTAC GGAAGCCGCT TTGCCATCCC TGCAGCCCAG 
CTAGTGGTGC TACCCTACCC AATGTTGCTG CATGCAGCTA CCCGACAGGC TGCGGGAATC 
CGGCTACAAG GTCAGGTGGT CATTATTGAC GAGGCTCACA ACCTGATCGA CACCATCACC 
AACATCCACA GCACAGAGGT CAATGGTTCC CAGCTCTGCC AGGCCCATTC CCAGTTGCTC 
CAGTACATGG AAAGATACAG GAAGCGTTTA AAGGCCAAGA ACTTGATGTA CATCAAGCAG 
ATCCTGTACT TGCTGGAGAA GTTTGTGGCT GTTTTGGGAG GTAATGTTAA GCAAAATCCT 
ACTACACAGA GCCTGTCTCA AACAGGGTCT GAGCTGAAGA GCATCAACGA CTTTCTCTTT 
CAGAGCCAGG TGGACAACAT CAACCTCTTC AAGGTGCAGC GGTACTTGGA GAAGAGCATG 
CTCAGCAGGA AGCTCTTTGG CTTCACAGAA TGTTTCGGAG TGGTTCTTCC ATCCCTCTCA 
GACTCTCAGG AAAACCGAGG TCTGGCTGGC TTCCAGCAGT TCCTGAAGAG CCTGCAGTCA 
GGGCCTACTG AGGACTCCCC GGAGGAGGGC CAGGCTGTAG CCCTGCGGCC TGCCTCTCCA 
CTGATGCACA TCGAGGCCTT CCTGGCAGCT CTCACCACAG CCAATCAGGA TGGCAGGGTT 
ATTGTGAACC GCCAAGGCAG TGTGGGTCAG AGCAGCCTTA AATTCTTACT CTTGAACCCA 
GCTGTGCACT TTGCCCAGGT GGTGAAGGAA TGCCGAGCAG TGGTCATTGC AGGTGGCACC 
ATGCAGCCGA CTTCTCTTAC ATTCACTACA AGAGCACACA ACCCCAGGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524473.1
CDS	185..2098
Translation	MADENQEIGGIHFPFPFPPYPIQKDFMAELYKVLEGGKIGIFESPTGTGKSLSLICGALSWLRDFEKKKLQAEALLLAPGSGPPSSEKNSLLTSSSCQEPTDTPRPAGEPDWVTEFVQKKEERDLVERLREEQVRRRKREERLKEVCQDGRLRFAAKRTKHEEEETEALLRLSREMLDAGTGPEQLEQLECGEEHLVLAEYESDEERRGSRVDEAEDDLEEEHITKIYYCSRTHSQLAQFVREVLKSPFGKETRLVSLGSRQTLCVNEDVKNLGSVQLMNDRCVDMQRSKREKNGTGEDKPKRKRQKIQTSCPFYNHEQMELLRDEILLEVKDMEQLVALGKEARACPYYGSRFAIPAAQLVVLPYPMLLHAATRQAAGIRLQGQVVIIDEAHNLIDTITNIHSTEVNGSQLCQAHSQLLQYMERYRKRLKAKNLMYIKQILYLLEKFVAVLGGNVKQNPTTQSLSQTGSELKSINDFLFQSQVDNINLFKVQRYLEKSMLSRKLFGFTECFGVVLPSLSDSQENRGLAGFQQFLKSLQSGPTEDSPEEGQAVALRPASPLMHIEAFLAALTTANQDGRVIVNRQGSVGQSSLKFLLLNPAVHFAQVVKECRAVVIAGGTMQPTSLTFTTRAHNPRL

Target ORF information:

RefSeq Version	XM_006524410.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (Ddx11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524410.4

ORF Insert Sequence:

ATGGCTGACG AAAACCAGGA GATTGGTGGC ATCCATTTTC CTTTTCCCTT CCCGCCCTAT 
CCTATCCAGA AAGACTTCAT GGCAGAGCTC TACAAGGTTT TGGAAGGTGG CAAGATTGGG 
ATATTTGAAA GTCCAACTGG CACGGGAAAG TCCTTAAGTC TGATTTGTGG GGCCCTTTCC 
TGGCTGCGTG ACTTTGAAAA GAAGAAATTG CAAGCAGAGG CTCTTCTCCT TGCACCTGGA 
TCCGGCCCTC CAAGTAGTGA GAAGAACTCG CTCCTGACTT CCTCATCTTG CCAAGAACCT 
ACGGACACCC CGAGGCCTGC TGGAGAGCCT GACTGGGTCA CCGAATTTGT GCAGAAGAAA 
GAAGAAAGGG ACCTGGTGGA GAGGCTAAGG GAGGAGCAGG TGAGGAGGAG GAAGCGAGAG 
GAGCGACTGA AGGAGGTCTG CCAGGATGGG AGGCTCAGGT TTGCAGCCAA GCGCACGAAA 
CATGAGGAAG AGGAGACTGA GGCTCTCCTG CGCCTCAGCA GGGAGATGCT GGATGCAGGG 
ACAGGACCAG AGCAGCTGGA GCAGCTGGAG TGTGGGGAGG AGCACCTGGT TCTGGCAGAA 
TATGAGAGTG ATGAGGAGAG AAGAGGCAGC AGGGTGGACG AGGCTGAGGA TGACTTGGAG 
GAAGAACATA TAACCAAGAT TTATTACTGC AGTCGGACAC ACTCACAGCT GGCCCAGTTT 
GTGCGGGAAG TACTGAAGAG CCCTTTTGGC AAGGAGACCC GGCTAGTCTC ACTTGGCTCT 
AGGCAGACCC TTTGTGTGAA TGAAGATGTG AAAAACCTGG GTTCTGTGCA ACTTATGAAT 
GACCGCTGTG TGGACATGCA AAGAAGCAAA CGCGAAAAGA ACGGGACTGG GGAAGACAAG 
CCAAAGAGAA AGAGACAGAA GATCCAGACC TCTTGCCCCT TCTACAACCA CGAGCAGATG 
GAGCTTCTCC GGGATGAGAT TCTGCTGGAA GTGAAGGACA TGGAACAACT TGTGGCCCTT 
GGGAAGGAGG CCCGGGCCTG CCCCTATTAC GGAAGCCGCT TTGCCATCCC TGCAGCCCAG 
CTAGTGGTGC TACCCTACCC AATGTTGCTG CATGCAGCTA CCCGACAGGC TGCGGGAATC 
CGGCTACAAG GTCAGGTGGT CATTATTGAC GAGGCTCACA ACCTGATCGA CACCATCACC 
AACATCCACA GCACAGAGGT CAATGGTTCC CAGCTCTGCC AGGCCCATTC CCAGTTGCTC 
CAGTACATGG AAAGATACAG GAAGCGTTTA AAGGCCAAGA ACTTGATGTA CATCAAGCAG 
ATCCTGTACT TGCTGGAGAA GTTTGTGGCT GTTTTGGGAG GTAATGTTAA GCAAAATCCT 
ACTACACAGA GCCTGTCTCA AACAGGGTCT GAGCTGAAGA GCATCAACGA CTTTCTCTTT 
CAGAGCCAGG TGGACAACAT CAACCTCTTC AAGGTGCAGC GGTACTTGGA GAAGAGCATG 
CTCAGCAGGA AGCTCTTTGG CTTCACAGAA TGTTTCGGAG TGGTTCTTCC ATCCCTCTCA 
GACTCTCAGG AAAACCGAGG TCTGGCTGGC TTCCAGCAGT TCCTGAAGAG CCTGCAGTCA 
GGGCCTACTG AGGACTCCCC GGAGGAGGGC CAGGCTGTAG CCCTGCGGCC TGCCTCTCCA 
CTGATGCACA TCGAGGCCTT CCTGGCAGCT CTCACCACAG CCAATCAGGA TGGCAGGGTT 
ATTGTGAACC GCCAAGGCAG TGTGGGTCAG AGCAGCCTTA AATTCTTACT CTTGAACCCA 
GCTGTGCACT TTGCCCAGGT GGTGAAGGAA TGCCGAGCAG TGGTCATTGC AGGTGGCACC 
ATGCAGCCGA CTTCTCTTAC ATTCACTACA AGAGCACACA ACCCCAGGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The ENU-induced cetus mutation reveals an essential role of the DNA helicase DDX11 for mesoderm development during early mouse embryogenesis.
Developmental dynamics : an official publication of the American Association of Anatomists241(8)1249-59(2012 Aug)
Cota CD,Garc?a-Garc?a MJ

Mammalian ChlR1 has a role in heterochromatin organization.
Experimental cell research317(17)2522-35(2011 Oct)
Inoue A,Hyle J,Lechner MS,Lahti JM

Loss of ChlR1 helicase in mouse causes lethality due to the accumulation of aneuploid cells generated by cohesion defects and placental malformation.
Cell cycle (Georgetown, Tex.)6(13)1646-54(2007 Jul)
Inoue A,Li T,Roby SK,Valentine MB,Inoue M,Boyd K,Kidd VJ,Lahti JM

Analysis of mouse embryonic patterning and morphogenesis by forward genetics.
Proceedings of the National Academy of Sciences of the United States of America102(17)5913-9(2005 Apr)
Garc?a-Garc?a MJ,Eggenschwiler JT,Caspary T,Alcorn HL,Wyler MR,Huangfu D,Rakeman AS,Lee JD,Feinberg EH,Timmer JR,Anderson KV

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Ddx11 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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