Efcab5 cDNA ORF clone, Mus musculus(house mouse)

The following Efcab5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Efcab5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu46455 XM_017314600.2
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu77942 XM_017314599.2
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu85815 XM_030246084.1
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu85816 XM_030246085.1
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu46451 XM_030246082.1
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu46451 XM_030246081.1
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu77938 XM_017314592.2
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu77940 XM_017314595.2
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu85812 XM_030246079.1
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu85813 XM_030246080.1
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu85814 XM_030246083.1
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu14808 NM_176965.3
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $825.30
$1179.00
OMu46451 XM_006533522.1
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OMu46452 XM_017314596.1
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OMu46452 XM_017314597.1
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OMu77939 XM_017314593.1
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OMu77940 XM_017314594.1
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OMu77941 XM_017314598.1
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OMu77943 XM_017314601.1
Latest version!
Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
View Old Accession Versions >>
OMu77938 XM_017314592.1 Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OMu46455 XM_017314600.1 Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OMu77940 XM_017314595.1 Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OMu77942 XM_017314599.1 Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu46455
    Clone ID Related Accession (Same CDS sequence) XM_017314600.1 , XM_017314600.2
    Accession Version XM_017314600.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314600.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCTTTA TGATTTTTTT CAGAAGCCAG AGCTGCATCT TGTACATTTC TTCATACATC 
    ACAGACTTGA AAAGCGAAGT CTTTGAGGAA TTCCTTAAAC ACCTGTGCCA CAGCGCAGAA
    GAATTCCGGG AGATCATACT GACTGACATG CGGAGGCAGA TGTTTGCAGA ACTCTTCCTA
    CAGTGCGACA GTGGGAAGGT GATGGCCTTG GATCGGCAGA GGACACTGGC CCTGCTGGAA
    GCGTTCTATG ATCAATGCTC TTCCACCACT AGGAGTTTAC TCCGAAACCC ACGACAATGG
    CCCTTTGTTG AATTTGAAGA GATAGAGTTG ATTGAGTTCT GGGGAGACAT GGATATTAAG
    AAGCACATTT ATGAAGACTT TGATGCACTG CTCTTAAAGA TGAATATGTT AGTTGCTGAA
    AAACTTGCAG GCAAACTCGC AGAAAACAAA GATCTCCCAC AGCAGCAGAG AGATCAAGAA
    CTTTCATCTG ACTCAACAAC GGAGCCAGAA ACTGCCACAC AACTGACCTC ACAACAGAGA
    TCCCGCAGAG TATCACTTAC AGGACAAGGA CAGGGCAAAG GGCCTCGCAA AACATCTGCT
    TCAAAACAAG GAGCAAGCAG AGGGTCAGTG GCAGAGCAAG GGTCCCGAAG AAGCTCGGGA
    GTGAACCAAA CCCAGCAGAG AGGGTCGGTG GCAGAACAAG GATCCCGAAG AAGCTCAGCA
    GTAGAACAAA CCCAGCAGAG AGGGTCGGTG GCAGAGCAAG GGTCCCGAAG AAGCTCTACA
    GTAGAACAAA CTCGGCAGAG AGGGTCAGTG GCAGAGCAAG GGTCCCGAAG AAGCTCTACA
    GTAGAACAAA CCCAGCGGAG AGGGTCGGTG GCAGAGCAAG GGTCCCGAAG AAGCTCGGGA
    GTGAACCAAA CCCAGCAGAG AGGGTCGGTG GCAGAACAAG GATCCCGAAG AAGCTCGGCA
    GTAGAACAAA CTCAGCAGAG AGGGTCGGTG GCAGAGCAAG GGTCCCGAAG AAGTTCAGCC
    ATGGAACAAG AGCCACAAAC AGCACAAGAT CCAAACAGCG ATTCATTGCC AGAACAAGAA
    TCACACAGAG GGTCCATCAC AGAAGGATCA CACAGAGGGT CAATTTCAGA ACCTGGGCAA
    CGCAGAGCAT CAGTAACAGG ACAACGCAGA AAGTCGTCTG TAGATGACTC CGGTAGTGCA
    GGGTCACGCA GAGGATCAGG GACAGATCAA GGACAGCACA GAGGATCCGT AGGGCAGCGC
    AAAGGGTCAG GAGAACGCAA AATGTCAGCT TCAGAGTATG GACCACATCA AGAGTCCATA
    ACAGAAGAGC CCCTCACTGC GTCAGAACCA GGACCGCAAA TAGACACTAT TCAAGAACTG
    GATGAAGACT CAACTCCACA ATTAGAAGAC GATAGTGCCC TAAAAGAAAG TAAAACCTCG
    GAACTCACTA AAATAGAAAC TCAAGAAGAA AAGCCTTTGC TGCTCATCAA CGAAGAGCAA
    GTCCCCAGAG ATTCCAAACA GCCAGAAGTT CCCACATCCA GCAAAAAGGA ACGTCCATCA
    GGCAGCCCCA AAAAAGGGCG CCTTTCAGGA ACTTCAAAAA GGGATTCTCA GAAAGACAAA
    GCCTGTGAAC CAAAGCCGCA ACATGTAGAA GGAAAAAAAT GGTCAGGAGA ATTTTTGATC
    TGTGACTGGA AGATAAAGCA TGTCAAATCT GAAGAGGAGG AACAGGCCAA GTTAATCTGT
    GATGACACCA GATTCACAGA CCTACATGCG ACTATTAGGA ATTTTCAGAC TTACAAGGGG
    ATCAAAGGGA GGAGTGCCTT CAATGGAGTC TCCCTCGATT TGCTGCAGTT TGTACAGCTC
    CTGGAGACAT TTGTTGGTGA AGACACCTCC TTCAGTCTCA GCAAGGACCT CGCCTCCTTT
    TTTCAGAAGA ACTATTCTGA AACAAAGCAA GAGAAAATTA AAGCCTTAGA ACAGGCCAGA
    CAAAATTCCT CCCGCATCCG ACGGAGAATT CTCCTCCAAG CCATCTTTGA GAAGTGGGAC
    AATGATGGCT CAGGCTTTCT GGATCTGAAT GAAGTTGATG ATCTCCTGTA TACATATAAG
    GAGGGAATGG AAAGAGAATC TATGAAGAAA GCTAAACTGC ACATAAATTT TCCAGAGCCA
    AGTCCCGGTC ACGAAGTTAA ACTGTCTTCA AAGCAATTTC AGAGGTACAT AGAACTGGTC
    GTATCTGAAC TCAGGGGCAA TGAAGACGAA GTTCTGGAAA GTGTTGTGGA ATTCCTGATG
    GGTTCTTTAG AGAGGAGCCA CGTTGAGGGT CTAAGGAACT GTGCAAGGCG GAAATGGCTC
    CATCAGATTC AGTACGCTGC AGAGACAAGT GGGGTGTCCC TGGAGCCGGT GTACACTGAG
    ACCTTCAGGG TCCTCACCCA GGACGCTGAG GCTCACGGAA ATAAGAAGAT CAGTGCTCAC
    ATCTCCCTCC TAGAGGAAAA CGTGTTCCTG CCTGAGAGAG GGCACGTTCT CTTGAGGAAC
    GTGGCTTGTA CTCTAGATGA CGCTCCGTTC GTCCTGAACA AAGTGCTCTA CAGGGACATG
    AAGGGAATCA GCTTTACAGT AGTGGATGAA GGAAAGCCAA TCCATGTCCC CCAAGTTCAG
    CACCATGGAA ATATCTTCTT CTGGAACAGT TTCCGAAGTA AGAATGAGTA TAACGGGTCG
    TTCCTGGCTT TGCCTCTTCA GGATGCATAC ATGAGGATCT TTGGGGTCCT GGCAGTTGAC
    ACGCTTCGAG ATCCTCATGA AATAAACATC TTCTTACCTC ATGAGATCAA ATTCTATCAG
    GGGGTCGCCA ATGCCTTCAG CACAGCTTAC CACCATGTTC ACAGCAGGGA ACACGTGCTG
    CACTCCGTGA TGACTGGCAT CCGGTGGCTC TTCAGTGTCA CATCTGGCAT CACCACCATC
    ACTACATGCT TCATCGAACC CAGCTCAGAA CAGGAAGACT ATGTTTTGCG CAATATGATG
    GTTACAGATT GTCTCGGTCT AGCAGAAATC CACACAGATC CCCCGACCAT CACGAGGAAC
    GCATGCATCT TTAGGGACTT CCTCTTTAAA TGCACAGACA CTTCAGAAGT TATTTTGGCT
    TCTTCCGGTG GAGAAACCCA CATTGCCATT CCACTTCGCC AGAGAACCAA AGAGGCTATG
    GGCATCCTCG ATGTTAACAT TGGCCGGAGC AGGATGCTGC TGTACCAAGA GTATAAAGAT
    CTACAGAAAA TGGTGAAGAT GATCCAAAAC GTCTCCTATG AAATACTGGG CGAATTCTCT
    GGCGAAATCG AGAAAACTAT GGTTATAGAG ATGGAATCGG CAGGAGAGGT GAAGCGGGCT
    GGGATCCTTT TCTTCCGAAC CATGCTGCAG GAGCTGCAGG AATGCCTCTG CCTCCTCGAT
    TCCATGGACT TTGTGTCGCT GCTTCTCTAT GAGCACAAAT ATCATGTCGA CTCCATCCTT
    CAGGACATCA CTCTCCAGGA GGTGGAAGCC AATGTGGCCC TGGTGCACGA CGTCCTGAAA
    GGAGTTATTC TGTTCTCTCA GCGGGAGAAA GACTCTTTAA GCGACTTGGA AGAGTGGGAA
    AAATGGAAGT TTCATATTAA CAAGTACTTA GTGGAGGAGA TTTGTGTGCT TGATCCGACT
    GCTAGCAACG TGGAGGTTAA TGTAGAGCTC GTTACAAGCT ATATCCAAGC TCATTCTCGG
    ACTGAAGTCT GGAATTTCAG GAACATTGTC ATTGAACTCC TGTATCACTG GATAAACATC
    TGTCTGACTC TCATAGAGCT AAACATGCGA CAAGATGTTT CCATTATCCC GCCATTGCCC
    AAGAAGAGTG CCACCTCTAT TTATGCAATA TCCTCAGAAA GATCAATCCG AGAGAAACTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170089.1
    CDS4881..8783
    Translation

    Target ORF information:

    RefSeq Version XM_017314600.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314600.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCTTTA TGATTTTTTT CAGAAGCCAG AGCTGCATCT TGTACATTTC TTCATACATC 
    ACAGACTTGA AAAGCGAAGT CTTTGAGGAA TTCCTTAAAC ACCTGTGCCA CAGCGCAGAA
    GAATTCCGGG AGATCATACT GACTGACATG CGGAGGCAGA TGTTTGCAGA ACTCTTCCTA
    CAGTGCGACA GTGGGAAGGT GATGGCCTTG GATCGGCAGA GGACACTGGC CCTGCTGGAA
    GCGTTCTATG ATCAATGCTC TTCCACCACT AGGAGTTTAC TCCGAAACCC ACGACAATGG
    CCCTTTGTTG AATTTGAAGA GATAGAGTTG ATTGAGTTCT GGGGAGACAT GGATATTAAG
    AAGCACATTT ATGAAGACTT TGATGCACTG CTCTTAAAGA TGAATATGTT AGTTGCTGAA
    AAACTTGCAG GCAAACTCGC AGAAAACAAA GATCTCCCAC AGCAGCAGAG AGATCAAGAA
    CTTTCATCTG ACTCAACAAC GGAGCCAGAA ACTGCCACAC AACTGACCTC ACAACAGAGA
    TCCCGCAGAG TATCACTTAC AGGACAAGGA CAGGGCAAAG GGCCTCGCAA AACATCTGCT
    TCAAAACAAG GAGCAAGCAG AGGGTCAGTG GCAGAGCAAG GGTCCCGAAG AAGCTCGGGA
    GTGAACCAAA CCCAGCAGAG AGGGTCGGTG GCAGAACAAG GATCCCGAAG AAGCTCAGCA
    GTAGAACAAA CCCAGCAGAG AGGGTCGGTG GCAGAGCAAG GGTCCCGAAG AAGCTCTACA
    GTAGAACAAA CTCGGCAGAG AGGGTCAGTG GCAGAGCAAG GGTCCCGAAG AAGCTCTACA
    GTAGAACAAA CCCAGCGGAG AGGGTCGGTG GCAGAGCAAG GGTCCCGAAG AAGCTCGGGA
    GTGAACCAAA CCCAGCAGAG AGGGTCGGTG GCAGAACAAG GATCCCGAAG AAGCTCGGCA
    GTAGAACAAA CTCAGCAGAG AGGGTCGGTG GCAGAGCAAG GGTCCCGAAG AAGTTCAGCC
    ATGGAACAAG AGCCACAAAC AGCACAAGAT CCAAACAGCG ATTCATTGCC AGAACAAGAA
    TCACACAGAG GGTCCATCAC AGAAGGATCA CACAGAGGGT CAATTTCAGA ACCTGGGCAA
    CGCAGAGCAT CAGTAACAGG ACAACGCAGA AAGTCGTCTG TAGATGACTC CGGTAGTGCA
    GGGTCACGCA GAGGATCAGG GACAGATCAA GGACAGCACA GAGGATCCGT AGGGCAGCGC
    AAAGGGTCAG GAGAACGCAA AATGTCAGCT TCAGAGTATG GACCACATCA AGAGTCCATA
    ACAGAAGAGC CCCTCACTGC GTCAGAACCA GGACCGCAAA TAGACACTAT TCAAGAACTG
    GATGAAGACT CAACTCCACA ATTAGAAGAC GATAGTGCCC TAAAAGAAAG TAAAACCTCG
    GAACTCACTA AAATAGAAAC TCAAGAAGAA AAGCCTTTGC TGCTCATCAA CGAAGAGCAA
    GTCCCCAGAG ATTCCAAACA GCCAGAAGTT CCCACATCCA GCAAAAAGGA ACGTCCATCA
    GGCAGCCCCA AAAAAGGGCG CCTTTCAGGA ACTTCAAAAA GGGATTCTCA GAAAGACAAA
    GCCTGTGAAC CAAAGCCGCA ACATGTAGAA GGAAAAAAAT GGTCAGGAGA ATTTTTGATC
    TGTGACTGGA AGATAAAGCA TGTCAAATCT GAAGAGGAGG AACAGGCCAA GTTAATCTGT
    GATGACACCA GATTCACAGA CCTACATGCG ACTATTAGGA ATTTTCAGAC TTACAAGGGG
    ATCAAAGGGA GGAGTGCCTT CAATGGAGTC TCCCTCGATT TGCTGCAGTT TGTACAGCTC
    CTGGAGACAT TTGTTGGTGA AGACACCTCC TTCAGTCTCA GCAAGGACCT CGCCTCCTTT
    TTTCAGAAGA ACTATTCTGA AACAAAGCAA GAGAAAATTA AAGCCTTAGA ACAGGCCAGA
    CAAAATTCCT CCCGCATCCG ACGGAGAATT CTCCTCCAAG CCATCTTTGA GAAGTGGGAC
    AATGATGGCT CAGGCTTTCT GGATCTGAAT GAAGTTGATG ATCTCCTGTA TACATATAAG
    GAGGGAATGG AAAGAGAATC TATGAAGAAA GCTAAACTGC ACATAAATTT TCCAGAGCCA
    AGTCCCGGTC ACGAAGTTAA ACTGTCTTCA AAGCAATTTC AGAGGTACAT AGAACTGGTC
    GTATCTGAAC TCAGGGGCAA TGAAGACGAA GTTCTGGAAA GTGTTGTGGA ATTCCTGATG
    GGTTCTTTAG AGAGGAGCCA CGTTGAGGGT CTAAGGAACT GTGCAAGGCG GAAATGGCTC
    CATCAGATTC AGTACGCTGC AGAGACAAGT GGGGTGTCCC TGGAGCCGGT GTACACTGAG
    ACCTTCAGGG TCCTCACCCA GGACGCTGAG GCTCACGGAA ATAAGAAGAT CAGTGCTCAC
    ATCTCCCTCC TAGAGGAAAA CGTGTTCCTG CCTGAGAGAG GGCACGTTCT CTTGAGGAAC
    GTGGCTTGTA CTCTAGATGA CGCTCCGTTC GTCCTGAACA AAGTGCTCTA CAGGGACATG
    AAGGGAATCA GCTTTACAGT AGTGGATGAA GGAAAGCCAA TCCATGTCCC CCAAGTTCAG
    CACCATGGAA ATATCTTCTT CTGGAACAGT TTCCGAAGTA AGAATGAGTA TAACGGGTCG
    TTCCTGGCTT TGCCTCTTCA GGATGCATAC ATGAGGATCT TTGGGGTCCT GGCAGTTGAC
    ACGCTTCGAG ATCCTCATGA AATAAACATC TTCTTACCTC ATGAGATCAA ATTCTATCAG
    GGGGTCGCCA ATGCCTTCAG CACAGCTTAC CACCATGTTC ACAGCAGGGA ACACGTGCTG
    CACTCCGTGA TGACTGGCAT CCGGTGGCTC TTCAGTGTCA CATCTGGCAT CACCACCATC
    ACTACATGCT TCATCGAACC CAGCTCAGAA CAGGAAGACT ATGTTTTGCG CAATATGATG
    GTTACAGATT GTCTCGGTCT AGCAGAAATC CACACAGATC CCCCGACCAT CACGAGGAAC
    GCATGCATCT TTAGGGACTT CCTCTTTAAA TGCACAGACA CTTCAGAAGT TATTTTGGCT
    TCTTCCGGTG GAGAAACCCA CATTGCCATT CCACTTCGCC AGAGAACCAA AGAGGCTATG
    GGCATCCTCG ATGTTAACAT TGGCCGGAGC AGGATGCTGC TGTACCAAGA GTATAAAGAT
    CTACAGAAAA TGGTGAAGAT GATCCAAAAC GTCTCCTATG AAATACTGGG CGAATTCTCT
    GGCGAAATCG AGAAAACTAT GGTTATAGAG ATGGAATCGG CAGGAGAGGT GAAGCGGGCT
    GGGATCCTTT TCTTCCGAAC CATGCTGCAG GAGCTGCAGG AATGCCTCTG CCTCCTCGAT
    TCCATGGACT TTGTGTCGCT GCTTCTCTAT GAGCACAAAT ATCATGTCGA CTCCATCCTT
    CAGGACATCA CTCTCCAGGA GGTGGAAGCC AATGTGGCCC TGGTGCACGA CGTCCTGAAA
    GGAGTTATTC TGTTCTCTCA GCGGGAGAAA GACTCTTTAA GCGACTTGGA AGAGTGGGAA
    AAATGGAAGT TTCATATTAA CAAGTACTTA GTGGAGGAGA TTTGTGTGCT TGATCCGACT
    GCTAGCAACG TGGAGGTTAA TGTAGAGCTC GTTACAAGCT ATATCCAAGC TCATTCTCGG
    ACTGAAGTCT GGAATTTCAG GAACATTGTC ATTGAACTCC TGTATCACTG GATAAACATC
    TGTCTGACTC TCATAGAGCT AAACATGCGA CAAGATGTTT CCATTATCCC GCCATTGCCC
    AAGAAGAGTG CCACCTCTAT TTATGCAATA TCCTCAGAAA GATCAATCCG AGAGAAACTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77942
    Clone ID Related Accession (Same CDS sequence) XM_017314599.1 , XM_017314599.2
    Accession Version XM_017314599.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314599.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGCTCATTCT CGGACTGAAG
    TCTGGAATTT CAGGAACATT GTCATTGAAC TCCTGTATCA CTGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170088.1
    CDS433..4500
    Translation

    Target ORF information:

    RefSeq Version XM_017314599.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314599.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGCTCATTCT CGGACTGAAG
    TCTGGAATTT CAGGAACATT GTCATTGAAC TCCTGTATCA CTGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85815
    Clone ID Related Accession (Same CDS sequence) XM_030246084.1
    Accession Version XM_030246084.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGCTCATTCT CGGACTGAAG
    TCTGGAATTT CAGGAACATT GTCATTGAAC TCCTGTATCA CTGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101944.1
    CDS433..4620
    Translation

    Target ORF information:

    RefSeq Version XM_030246084.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246084.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGCTCATTCT CGGACTGAAG
    TCTGGAATTT CAGGAACATT GTCATTGAAC TCCTGTATCA CTGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85816
    Clone ID Related Accession (Same CDS sequence) XM_030246085.1
    Accession Version XM_030246085.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3288bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG GACGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101945.1
    CDS444..3731
    Translation

    Target ORF information:

    RefSeq Version XM_030246085.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246085.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG GACGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu46451
    Clone ID Related Accession (Same CDS sequence) XM_030246082.1 , XM_030246081.1 , XM_006533522.1
    Accession Version XM_030246082.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT 
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101942.1
    CDS4067..8758
    Translation

    Target ORF information:

    RefSeq Version XM_030246082.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246082.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT 
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu46451
    Clone ID Related Accession (Same CDS sequence) XM_030246082.1 , XM_030246081.1 , XM_006533522.1
    Accession Version XM_030246081.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT 
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101941.1
    CDS4163..8854
    Translation

    Target ORF information:

    RefSeq Version XM_030246081.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246081.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT 
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77938
    Clone ID Related Accession (Same CDS sequence) XM_017314592.2 , XM_017314592.1
    Accession Version XM_017314592.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170081.1
    CDS428..5359
    Translation

    Target ORF information:

    RefSeq Version XM_017314592.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314592.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77940
    Clone ID Related Accession (Same CDS sequence) XM_017314594.1 , XM_017314595.1 , XM_017314595.2
    Accession Version XM_017314595.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTTAAAA AGCCTGCAAA AGTGATTCTT GGTATAGACG AAAGTGATCT GAGGTTCAAA 
    GTACAGCTGC CGTGGAAGAA AAATCTTAAT TTGAGGATAG AGGAAAGAGC CCAGGCCAAG
    CAACAGAAAA TATTAGAGAA CGATAAACTG AAGAAGGAAC AGGAAAAGAA GGTTACAAAA
    AAGCTCCCTA GGGATGACTT GGCTAAAGAG TGGTTCAATC CTGAAAGCAT GACACTGAAT
    ACCCGGGCGT ACCTGCTGGA CAAGCTTCTA CCCACCTTGG TTCCCGGAGT GGAGCAAATG
    CTGATGCAGG TGGAGAAGAA GAAGCTTTTG AGCCAAGCTG ACATCCCAAC CAAGTTTGAT
    CCAATTAATT ATTTGGGGGA ATATTTAATG AGACACAACA CTCATTATAT CAAAGACCCA
    GGAGTGTCTG GCTACCAGAG GGTGATGAAA GAGATCACTG AAGAACTGAA GACTCATGTC
    CCTGATACGA TCAACAACAG AATATCCAAG ATGAAGGAGA AAGTGAAAGA GAAGAGAGAA
    CAAAGAGAGT ACATAAGCAC AGTCAAAGTC AAGGTGGCCG GCATGAGAAA GCAGGCTCTG
    GAAGAGCAGT TCAATGAATG GGTTCTCAAC CCCAAAGGGA TGATTCCAAT AGTCGTGATT
    CAGAATGTCC TTTATGATTT TTTTCAGAAG CCAGAGCTGC ATCTTGAATC ACGTTGCAAA
    GATCTGGTTA TTATGAAATC GGTGGATCTA GGATTGAACC AACAGCAGTT TGTACAATAC
    ATTTCTTCAT ACATCACAGA CTTGAAAAGC GAAGTCTTTG AGGAATTCCT TAAACACCTG
    TGCCACAGCG CAGAAGAATT CCGGGAGATC ATACTGACTG ACATGCGGAG GCAGATGTTT
    GCAGAACTCT TCCTACAGTG CGACAGTGGG AAGGTGATGG CCTTGGATCG GCAGAGGACA
    CTGGCCCTGC TGGAAGCGTT CTATGATCAA TGCTCTTCCA CCACTAGGAG TTTACTCCGA
    AACCCACGAC AATGGCCCTT TGTTGAATTT GAAGAGATAG AGTTGATTGA GTTCTGGGGA
    GACATGGATA TTAAGAAGCA CATTTATGAA GACTTTGATG CACTGCTCTT AAAGATGAAT
    ATGTTAGTTG CTGAAAAACT TGCAGGCAAA CTCGCAGAAA ACAAAGATCT CCCACAGCAG
    CAGAGAGATC AAGAACTTTC ATCTGACTCA ACAACGGAGC CAGAAACTGC CACACAACTG
    ACCTCACAAC AGAGATCCCG CAGAGTATCA CTTACAGGAC AAGGACAGGG CAAAGGGCCT
    CGCAAAACAT CTGCTTCAAA ACAAGGAGCA AGCAGAGGGT CAGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CGGGAGTGAA CCAAACCCAG CAGAGAGGGT CGGTGGCAGA ACAAGGATCC
    CGAAGAAGCT CAGCAGTAGA ACAAACCCAG CAGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CTACAGTAGA ACAAACTCGG CAGAGAGGGT CAGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CTACAGTAGA ACAAACCCAG CGGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CGGGAGTGAA CCAAACCCAG CAGAGAGGGT CGGTGGCAGA ACAAGGATCC
    CGAAGAAGCT CGGCAGTAGA ACAAACTCAG CAGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGTT CAGCCATGGA ACAAGAGCCA CAAACAGCAC AAGATCCAAA CAGCGATTCA
    TTGCCAGAAC AAGAATCACA CAGAGGGTCC ATCACAGAAG GATCACACAG AGGGTCAATT
    TCAGAACCTG GGCAACGCAG AGCATCAGTA ACAGGACAAC GCAGAAAGTC GTCTGTAGAT
    GACTCCGGTA GTGCAGGGTC ACGCAGAGGA TCAGGGACAG ATCAAGGACA GCACAGAGGA
    TCCGTAGGGC AGCGCAAAGG GTCAGGAGAA CGCAAAATGT CAGCTTCAGA GTATGGACCA
    CATCAAGAGT CCATAACAGA AGAGCCCCTC ACTGCGTCAG AACCAGGACC GCAAATAGAC
    ACTATTCAAG AACTGGATGA AGACTCAACT CCACAATTAG AAGACGATAG TGCCCTAAAA
    GAAAGTAAAA CCTCGGAACT CACTAAAATA GAAACTCAAG AAGAAAAGCC TTTGCTGCTC
    ATCAACGAAG AGCAAGTCCC CAGAGATTCC AAACAGCCAG AAGTTCCCAC ATCCAGCAAA
    AAGGAACGTC CATCAGGCAG CCCCAAAAAA GGGCGCCTTT CAGGAACTTC AAAAAGGGAT
    TCTCAGAAAG ACAAAGCCTG TGAACCAAAG CCGCAACATG TAGAAGGAAA AAAATGGTCA
    GGAGAATTTT TGATCTGTGA CTGGAAGATA AAGCATGTCA AATCTGAAGA GGAGGAACAG
    GCCAAGTTAA TCTGTGATGA CACCAGATTC ACAGACCTAC ATGCGACTAT TAGGAATTTT
    CAGACTTACA AGGGGATCAA AGGGAGGAGT GCCTTCAATG GAGTCTCCCT CGATTTGCTG
    CAGTTTGTAC AGCTCCTGGA GACATTTGTT GGTGAAGACA CCTCCTTCAG TCTCAGCAAG
    GACCTCGCCT CCTTTTTTCA GAAGAACTAT TCTGAAACAA AGCAAGAGAA AATTAAAGCC
    TTAGAACAGG CCAGACAAAA TTCCTCCCGC ATCCGACGGA GAATTCTCCT CCAAGCCATC
    TTTGAGAAGT GGGACAATGA TGGCTCAGGC TTTCTGGATC TGAATGAAGT TGATGATCTC
    CTGTATACAT ATAAGGAGGG AATGGAAAGA GAATCTATGA AGAAAGCTAA ACTGCACATA
    AATTTTCCAG AGCCAAGTCC CGGTCACGAA GTTAAACTGT CTTCAAAGCA ATTTCAGAGG
    TACATAGAAC TGGTCGTATC TGAACTCAGG GGCAATGAAG ACGAAGTTCT GGAAAGTGTT
    GTGGAATTCC TGATGGGTTC TTTAGAGAGG AGCCACGTTG AGGGTCTAAG GAACTGTGCA
    AGGCGGAAAT GGCTCCATCA GATTCAGTAC GCTGCAGAGA CAAGTGGGGT GTCCCTGGAG
    CCGGTGTACA CTGAGACCTT CAGGGTCCTC ACCCAGGACG CTGAGGCTCA CGGAAATAAG
    AAGATCAGTG CTCACATCTC CCTCCTAGAG GAAAACGTGT TCCTGCCTGA GAGAGGGCAC
    GTTCTCTTGA GGAACGTGGC TTGTACTCTA GATGACGCTC CGTTCGTCCT GAACAAAGTG
    CTCTACAGGG ACATGAAGGG AATCAGCTTT ACAGTAGTGG ATGAAGGAAA GCCAATCCAT
    GTCCCCCAAG TTCAGCACCA TGGAAATATC TTCTTCTGGA ACAGTTTCCG AAGTAAGAAT
    GAGTATAACG GGTCGTTCCT GGCTTTGCCT CTTCAGGATG CATACATGAG GATCTTTGGG
    GTCCTGGCAG TTGACACGCT TCGAGATCCT CATGAAATAA ACATCTTCTT ACCTCATGAG
    ATCAAATTCT ATCAGGGGGT CGCCAATGCC TTCAGCACAG CTTACCACCA TGTTCACAGC
    AGGGAACACG TGCTGCACTC CGTGATGACT GGCATCCGGT GGCTCTTCAG TGTCACATCT
    GGCATCACCA CCATCACTAC ATGCTTCATC GAACCCAGCT CAGAACAGGA AGACTATGTT
    TTGCGCAATA TGATGGTTAC AGATTGTCTC GGTCTAGCAG AAATCCACAC AGATCCCCCG
    ACCATCACGA GGAACGCATG CATCTTTAGG GACTTCCTCT TTAAATGCAC AGACACTTCA
    GAAGTTATTT TGGCTTCTTC CGGTGGAGAA ACCCACATTG CCATTCCACT TCGCCAGAGA
    ACCAAAGAGG CTATGGGCAT CCTCGATGTT AACATTGGCC GGAGCAGGAT GCTGCTGTAC
    CAAGAGTATA AAGATCTACA GAAAATGGTG AAGATGATCC AAAACGTCTC CTATGAAATA
    CTGGGCGAAT TCTCTGGCGA AATCGAGAAA ACTATGGTTA TAGAGATGGA ATCGGCAGGA
    GAGGTGAAGC GGGCTGGGAT CCTTTTCTTC CGAACCATGC TGCAGGAGCT GCAGGAATGC
    CTCTGCCTCC TCGATTCCAT GGACTTTGTG TCGCTGCTTC TCTATGAGCA CAAATATCAT
    GTCGACTCCA TCCTTCAGGA CATCACTCTC CAGGAGGTGG AAGCCAATGT GGCCCTGGTG
    CACGACGTCC TGAAAGGAGT TATTCTGTTC TCTCAGCGGG AGAAAGACTC TTTAAGCGAC
    TTGGAAGAGT GGGAAAAATG GAAGTTTCAT ATTAACAAGT ACTTAGTGGA GGAGATTTGT
    GTGCTTGATC CGACTGCTAG CAACGTGGAG GTTAATGTAG AGCTCGTTAC AAGCTATATC
    CAAGCTCATT CTCGGACTGA AGTCTGGAAT TTCAGGAACA TTGTCATTGA ACTCCTGTAT
    CACTGGATAA ACATCTGTCT GACTCTCATA GAGCTAAACA TGCGACAAGA TGTTTCCATT
    ATCCCGCCAT TGCCCAAGAA GAGTGCCACC TCTATTTATG CAATATCCTC AGAAAGATCA
    ATCCGAGAGA AACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170084.1
    CDS1095..5732
    Translation

    Target ORF information:

    RefSeq Version XM_017314595.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314595.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTTAAAA AGCCTGCAAA AGTGATTCTT GGTATAGACG AAAGTGATCT GAGGTTCAAA 
    GTACAGCTGC CGTGGAAGAA AAATCTTAAT TTGAGGATAG AGGAAAGAGC CCAGGCCAAG
    CAACAGAAAA TATTAGAGAA CGATAAACTG AAGAAGGAAC AGGAAAAGAA GGTTACAAAA
    AAGCTCCCTA GGGATGACTT GGCTAAAGAG TGGTTCAATC CTGAAAGCAT GACACTGAAT
    ACCCGGGCGT ACCTGCTGGA CAAGCTTCTA CCCACCTTGG TTCCCGGAGT GGAGCAAATG
    CTGATGCAGG TGGAGAAGAA GAAGCTTTTG AGCCAAGCTG ACATCCCAAC CAAGTTTGAT
    CCAATTAATT ATTTGGGGGA ATATTTAATG AGACACAACA CTCATTATAT CAAAGACCCA
    GGAGTGTCTG GCTACCAGAG GGTGATGAAA GAGATCACTG AAGAACTGAA GACTCATGTC
    CCTGATACGA TCAACAACAG AATATCCAAG ATGAAGGAGA AAGTGAAAGA GAAGAGAGAA
    CAAAGAGAGT ACATAAGCAC AGTCAAAGTC AAGGTGGCCG GCATGAGAAA GCAGGCTCTG
    GAAGAGCAGT TCAATGAATG GGTTCTCAAC CCCAAAGGGA TGATTCCAAT AGTCGTGATT
    CAGAATGTCC TTTATGATTT TTTTCAGAAG CCAGAGCTGC ATCTTGAATC ACGTTGCAAA
    GATCTGGTTA TTATGAAATC GGTGGATCTA GGATTGAACC AACAGCAGTT TGTACAATAC
    ATTTCTTCAT ACATCACAGA CTTGAAAAGC GAAGTCTTTG AGGAATTCCT TAAACACCTG
    TGCCACAGCG CAGAAGAATT CCGGGAGATC ATACTGACTG ACATGCGGAG GCAGATGTTT
    GCAGAACTCT TCCTACAGTG CGACAGTGGG AAGGTGATGG CCTTGGATCG GCAGAGGACA
    CTGGCCCTGC TGGAAGCGTT CTATGATCAA TGCTCTTCCA CCACTAGGAG TTTACTCCGA
    AACCCACGAC AATGGCCCTT TGTTGAATTT GAAGAGATAG AGTTGATTGA GTTCTGGGGA
    GACATGGATA TTAAGAAGCA CATTTATGAA GACTTTGATG CACTGCTCTT AAAGATGAAT
    ATGTTAGTTG CTGAAAAACT TGCAGGCAAA CTCGCAGAAA ACAAAGATCT CCCACAGCAG
    CAGAGAGATC AAGAACTTTC ATCTGACTCA ACAACGGAGC CAGAAACTGC CACACAACTG
    ACCTCACAAC AGAGATCCCG CAGAGTATCA CTTACAGGAC AAGGACAGGG CAAAGGGCCT
    CGCAAAACAT CTGCTTCAAA ACAAGGAGCA AGCAGAGGGT CAGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CGGGAGTGAA CCAAACCCAG CAGAGAGGGT CGGTGGCAGA ACAAGGATCC
    CGAAGAAGCT CAGCAGTAGA ACAAACCCAG CAGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CTACAGTAGA ACAAACTCGG CAGAGAGGGT CAGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CTACAGTAGA ACAAACCCAG CGGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CGGGAGTGAA CCAAACCCAG CAGAGAGGGT CGGTGGCAGA ACAAGGATCC
    CGAAGAAGCT CGGCAGTAGA ACAAACTCAG CAGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGTT CAGCCATGGA ACAAGAGCCA CAAACAGCAC AAGATCCAAA CAGCGATTCA
    TTGCCAGAAC AAGAATCACA CAGAGGGTCC ATCACAGAAG GATCACACAG AGGGTCAATT
    TCAGAACCTG GGCAACGCAG AGCATCAGTA ACAGGACAAC GCAGAAAGTC GTCTGTAGAT
    GACTCCGGTA GTGCAGGGTC ACGCAGAGGA TCAGGGACAG ATCAAGGACA GCACAGAGGA
    TCCGTAGGGC AGCGCAAAGG GTCAGGAGAA CGCAAAATGT CAGCTTCAGA GTATGGACCA
    CATCAAGAGT CCATAACAGA AGAGCCCCTC ACTGCGTCAG AACCAGGACC GCAAATAGAC
    ACTATTCAAG AACTGGATGA AGACTCAACT CCACAATTAG AAGACGATAG TGCCCTAAAA
    GAAAGTAAAA CCTCGGAACT CACTAAAATA GAAACTCAAG AAGAAAAGCC TTTGCTGCTC
    ATCAACGAAG AGCAAGTCCC CAGAGATTCC AAACAGCCAG AAGTTCCCAC ATCCAGCAAA
    AAGGAACGTC CATCAGGCAG CCCCAAAAAA GGGCGCCTTT CAGGAACTTC AAAAAGGGAT
    TCTCAGAAAG ACAAAGCCTG TGAACCAAAG CCGCAACATG TAGAAGGAAA AAAATGGTCA
    GGAGAATTTT TGATCTGTGA CTGGAAGATA AAGCATGTCA AATCTGAAGA GGAGGAACAG
    GCCAAGTTAA TCTGTGATGA CACCAGATTC ACAGACCTAC ATGCGACTAT TAGGAATTTT
    CAGACTTACA AGGGGATCAA AGGGAGGAGT GCCTTCAATG GAGTCTCCCT CGATTTGCTG
    CAGTTTGTAC AGCTCCTGGA GACATTTGTT GGTGAAGACA CCTCCTTCAG TCTCAGCAAG
    GACCTCGCCT CCTTTTTTCA GAAGAACTAT TCTGAAACAA AGCAAGAGAA AATTAAAGCC
    TTAGAACAGG CCAGACAAAA TTCCTCCCGC ATCCGACGGA GAATTCTCCT CCAAGCCATC
    TTTGAGAAGT GGGACAATGA TGGCTCAGGC TTTCTGGATC TGAATGAAGT TGATGATCTC
    CTGTATACAT ATAAGGAGGG AATGGAAAGA GAATCTATGA AGAAAGCTAA ACTGCACATA
    AATTTTCCAG AGCCAAGTCC CGGTCACGAA GTTAAACTGT CTTCAAAGCA ATTTCAGAGG
    TACATAGAAC TGGTCGTATC TGAACTCAGG GGCAATGAAG ACGAAGTTCT GGAAAGTGTT
    GTGGAATTCC TGATGGGTTC TTTAGAGAGG AGCCACGTTG AGGGTCTAAG GAACTGTGCA
    AGGCGGAAAT GGCTCCATCA GATTCAGTAC GCTGCAGAGA CAAGTGGGGT GTCCCTGGAG
    CCGGTGTACA CTGAGACCTT CAGGGTCCTC ACCCAGGACG CTGAGGCTCA CGGAAATAAG
    AAGATCAGTG CTCACATCTC CCTCCTAGAG GAAAACGTGT TCCTGCCTGA GAGAGGGCAC
    GTTCTCTTGA GGAACGTGGC TTGTACTCTA GATGACGCTC CGTTCGTCCT GAACAAAGTG
    CTCTACAGGG ACATGAAGGG AATCAGCTTT ACAGTAGTGG ATGAAGGAAA GCCAATCCAT
    GTCCCCCAAG TTCAGCACCA TGGAAATATC TTCTTCTGGA ACAGTTTCCG AAGTAAGAAT
    GAGTATAACG GGTCGTTCCT GGCTTTGCCT CTTCAGGATG CATACATGAG GATCTTTGGG
    GTCCTGGCAG TTGACACGCT TCGAGATCCT CATGAAATAA ACATCTTCTT ACCTCATGAG
    ATCAAATTCT ATCAGGGGGT CGCCAATGCC TTCAGCACAG CTTACCACCA TGTTCACAGC
    AGGGAACACG TGCTGCACTC CGTGATGACT GGCATCCGGT GGCTCTTCAG TGTCACATCT
    GGCATCACCA CCATCACTAC ATGCTTCATC GAACCCAGCT CAGAACAGGA AGACTATGTT
    TTGCGCAATA TGATGGTTAC AGATTGTCTC GGTCTAGCAG AAATCCACAC AGATCCCCCG
    ACCATCACGA GGAACGCATG CATCTTTAGG GACTTCCTCT TTAAATGCAC AGACACTTCA
    GAAGTTATTT TGGCTTCTTC CGGTGGAGAA ACCCACATTG CCATTCCACT TCGCCAGAGA
    ACCAAAGAGG CTATGGGCAT CCTCGATGTT AACATTGGCC GGAGCAGGAT GCTGCTGTAC
    CAAGAGTATA AAGATCTACA GAAAATGGTG AAGATGATCC AAAACGTCTC CTATGAAATA
    CTGGGCGAAT TCTCTGGCGA AATCGAGAAA ACTATGGTTA TAGAGATGGA ATCGGCAGGA
    GAGGTGAAGC GGGCTGGGAT CCTTTTCTTC CGAACCATGC TGCAGGAGCT GCAGGAATGC
    CTCTGCCTCC TCGATTCCAT GGACTTTGTG TCGCTGCTTC TCTATGAGCA CAAATATCAT
    GTCGACTCCA TCCTTCAGGA CATCACTCTC CAGGAGGTGG AAGCCAATGT GGCCCTGGTG
    CACGACGTCC TGAAAGGAGT TATTCTGTTC TCTCAGCGGG AGAAAGACTC TTTAAGCGAC
    TTGGAAGAGT GGGAAAAATG GAAGTTTCAT ATTAACAAGT ACTTAGTGGA GGAGATTTGT
    GTGCTTGATC CGACTGCTAG CAACGTGGAG GTTAATGTAG AGCTCGTTAC AAGCTATATC
    CAAGCTCATT CTCGGACTGA AGTCTGGAAT TTCAGGAACA TTGTCATTGA ACTCCTGTAT
    CACTGGATAA ACATCTGTCT GACTCTCATA GAGCTAAACA TGCGACAAGA TGTTTCCATT
    ATCCCGCCAT TGCCCAAGAA GAGTGCCACC TCTATTTATG CAATATCCTC AGAAAGATCA
    ATCCGAGAGA AACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85812
    Clone ID Related Accession (Same CDS sequence) XM_030246079.1
    Accession Version XM_030246079.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101939.1
    CDS429..5480
    Translation

    Target ORF information:

    RefSeq Version XM_030246079.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246079.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85813
    Clone ID Related Accession (Same CDS sequence) XM_030246080.1
    Accession Version XM_030246080.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4812bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101940.1
    CDS428..5239
    Translation

    Target ORF information:

    RefSeq Version XM_030246080.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246080.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85814
    Clone ID Related Accession (Same CDS sequence) XM_030246083.1
    Accession Version XM_030246083.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGCTC ATTCTCGGAC TGAAGTCTGG
    AATTTCAGGA ACATTGTCAT TGAACTCCTG TATCACTGGA TAAACATCTG TCTGACTCTC
    ATAGAGCTAA ACATGCGACA AGATGTTTCC ATTATCCCGC CATTGCCCAA GAAGAGTGCC
    ACCTCTATTT ATGCAATATC CTCAGAAAGA TCAATCCGAG AGAAACTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101943.1
    CDS430..5100
    Translation

    Target ORF information:

    RefSeq Version XM_030246083.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246083.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGCTC ATTCTCGGAC TGAAGTCTGG
    AATTTCAGGA ACATTGTCAT TGAACTCCTG TATCACTGGA TAAACATCTG TCTGACTCTC
    ATAGAGCTAA ACATGCGACA AGATGTTTCC ATTATCCCGC CATTGCCCAA GAAGAGTGCC
    ACCTCTATTT ATGCAATATC CTCAGAAAGA TCAATCCGAG AGAAACTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu14808
    Clone ID Related Accession (Same CDS sequence) NM_176965.3
    Accession Version NM_176965.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-09
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DT929470.1, AK029790.1 and AK163359.1. On Nov 16, 2006 this sequence version replaced NM_176965.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCTGATGC AGGTGGAGAA GAAGAAGCTT TTGAGCCAAG CTGACATCCC AACCAAGTTT 
    GATCCAATTA ATTATTTGGG GGAATATTTA ATGAGACACA ACACTCATTA TATCAAAGAC
    CCAGGAGTGT CTGGCTACCA GAGGGTGATG AAAGAGATCA CTGAAGAACT GAAGACTCAT
    GTCCCTGATA CGATCAACAA CAGAATATCC AAGATGAAGG AGAAAGTGAA AGAGAAGAGA
    GAACAAAGAG AGTACATAAG CACAGTCAAA GTCAAGGTGG CCGGCATGAG AAAGCAGGCT
    CTGGAAGAGC AGTTCAATGA ATGGGTTCTC AACCCCAAAG GGATGATTCC AATAGTCGTG
    TACATTTCTT CATACATCAC AGACTTGAAA AGCGAAGTCT TTGAGGAATT CCTTAAACAC
    CTGTGCCACA GCGCAGAAGA ATTCCGGGAG ATCATACTGA CTGACATGCG GAGGCAGATG
    TTTGCAGAAC TCTTCCTACA GTGCGACAGT GGGAAGGTGA TGGCCTTGGA TCGGCAGAGG
    ACACTGGCCC TGCTGGAAGC GTTCTATGAT CAATGCTCTT CCACCACTAG GAGTTTACTC
    CGAAACCCAC GACAATGGCC CTTTGTTGAA TTTGAAGAGA TAGAGTTGAT TGAGTTCTGG
    GGAGACATGG ATATTAAGAA GCACATTTAT GAAGACTTTG ATGCACTGCT CTTAAAGATG
    AATATGTTAG TTGCTGAAAA ACTTGCAGGC AAACTCGCAG AAAACAAAGA TCTCCCACAG
    CAGCAGAGAG ATCAAGAACT TTCATCTGAC TCAACAACGG AGCCAGAAAC TGCCACACAA
    CTGACCTCAC AACAGAGATC CCGCAGAGTA TCACTTACAG GACAAGGACA GGGCAAAGGG
    CCTCGCAAAA CATCTGCTTC AAAACAAGGA GCAAGCAGAG GGTCAGTGGC AGAGCAAGGG
    TCCCGAAGAA GCTCGGGAGT GAACCAAACC CAGCAGAGAG GGTCGGTGGC AGAACAAGGA
    TCCCGAAGAA GCTCAGCAGT AGAACAAACC CAGCAGAGAG GGTCGGTGGC AGAGCAAGGG
    TCCCGAAGAA GCTCTACAGT AGAACAAACT CGGCAGAGAG GGTCAGTGGC AGAGCAAGGG
    TCCCGAAGAA GCTCTACAGT AGAACAAACC CAGCGGAGAG GGTCGGTGGC AGAGCAAGGG
    TCCCGAAGAA GCTCGGGAGT GAACCAAACC CAGCAGAGAG GGTCGGTGGC AGAACAAGGA
    TCCCGAAGAA GCTCGGCAGT AGAACAAACT CAGCAGAGAG GGTCGGTGGC AGAGCAAGGG
    TCCCGAAGAA GTTCAGCCAT GGAACAAGAG CCACAAACAG CACAAGATCC AAACAGCGAT
    TCATTGCCAG AACAAGAATC ACACAGAGGG TCCATCACAG AAGGATCACA CAGAGGGTCA
    ATTTCAGAAC CTGGGCAACG CAGAGCATCA GTAACAGGAC AACGCAGAAA GTCGTCTGTA
    GATGACTCCG GTAGTGCAGG GTCACGCAGA GGATCAGGGA CAGATCAAGG ACAGCACAGA
    GGATCCGTAG GGCAGCGCAA AGGGTCAGGA GAACGCAAAA TGTCAGCTTC AGAGTATGGA
    CCACATCAAG AGTCCATAAC AGAAGAGCCC CTCACTGCGT CAGAACCAGG ACCGCAAATA
    GACACTATTC AAGAACTGGA TGAAGACTCA ACTCCACAAT TAGAAGACGA TAGTGCCCTA
    AAAGAAAGTA AAACCTCGGA ACTCACTAAA ATAGAAACTC AAGAAGAAAA GCCTTTGCTG
    CTCATCAACG AAGAGCAAGT CCCCAGAGAT TCCAAACAGC CAGAAGTTCC CACATCCAGC
    AAAAAGGAAC GTCCATCAGG CAGCCCCAAA AAAGGGCGCC TTTCAGGAAC TTCAAAAAGG
    GATTCTCAGA AAGACAAAGC CTGTGAACCA AAGCCGCAAC ATGTAGAAGG AAAAAAATGG
    TCAGGAGAAT TTTTGATCTG TGACTGGAAG ATAAAGCATG TCAAATCTGA AGAGGAGGAA
    CAGGCCAAGT TAATCTGTGA TGACACCAGA TTCACAGACC TACATGCGAC TATTAGGAAT
    TTTCAGACTT ACAAGGGGAT CAAAGGGAGG AGTGCCTTCA ATGGAGTCTC CCTCGATTTG
    CTGCAGTTTG TACAGCTCCT GGAGACATTT GTTGGTGAAG ACACCTCCTT CAGTCTCAGC
    AAGGACCTCG CCTCCTTTTT TCAGAAGAAC TATTCTGAAA CAAAGCAAGA GAAAATTAAA
    GCCTTAGAAC AGGCCAGACA AAATTCCTCC CGCATCCGAC GGAGAATTCT CCTCCAAGCC
    ATCTTTGAGA AGTGGGACAA TGATGGCTCA GGCTTTCTGG ATCTGAATGA AGTTGATGAT
    CTCCTGTATA CATATAAGGA GGGAATGGAA AGAGAATCTA TGAAGAAAGC TAAACTGCAC
    ATAAATTTTC CAGAGCCAAG TCCCGGTCAC GAAGTTAAAC TGTCTTCAAA GCAATTTCAG
    AGGTACATAG AACTGGTCGT ATCTGAACTC AGGGGCAATG AAGACGAAGT TCTGGAAAGT
    GTTGTGGAAT TCCTGATGGG TTCTTTAGAG AGGAGCCACG TTGAGGGTCT AAGGAACTGT
    GCAAGGCGGA AATGGCTCCA TCAGATTCAG TACGCTGCAG AGACAAGTGG GGTGTCCCTG
    GAGCCGGTGT ACACTGAGAC CTTCAGGGTC CTCACCCAGG ACGCTGAGGC TCACGGAAAT
    AAGAAGATCA GTGCTCACAT CTCCCTCCTA GAGGAAAACG TGTTCCTGCC TGAGAGAGGG
    CACGTTCTCT TGAGGAACGT GGCTTGTACT CTAGATGACG CTCCGTTCGT CCTGAACAAA
    GTGCTCTACA GGGACATGAA GGGAATCAGC TTTACAGTAG TGGATGAAGG AAAGCCAATC
    CATGTCCCCC AAGTTCAGCA CCATGGAAAT ATCTTCTTCT GGAACAGTTT CCGAAGTAAG
    AATGAGTATA ACGGGTCGTT CCTGGCTTTG CCTCTTCAGG ATGCATACAT GAGGATCTTT
    GGGGTCCTGG CAGTTGACAC GCTTCGAGAT CCTCATGAAA TAAACATCTT CTTACCTCAT
    GAGATCAAAT TCTATCAGGG GGTCGCCAAT GCCTTCAGCA CAGCTTACCA CCATGTTCAC
    AGCAGGGAAC ACGTGCTGCA CTCCGTGATG ACTGGCATCC GGTGGCTCTT CAGTGTCACA
    TCTGGCATCA CCACCATCAC TACATGCTTC ATCGAACCCA GCTCAGAACA GGAAGACTAT
    GTTTTGCGCA ATATGATGGT TACAGATTGT CTCGGTCTAG CAGAAATCCA CACAGATCCC
    CCGACCATCA CGAGGAACGC ATGCATCTTT AGGGACTTCC TCTTTAAATG CACAGACACT
    TCAGAAGTTA TTTTGGCTTC TTCCGGTGGA GAAACCCACA TTGCCATTCC ACTTCGCCAG
    AGAACCAAAG AGGCTATGGG CATCCTCGAT GTTAACATTG GCCGGAGCAG GATGCTGCTG
    TACCAAGAGT ATAAAGATCT ACAGAAAATG GTGAAGATGA TCCAAAACGT CTCCTATGAA
    ATACTGGGCG AATTCTCTGG CGAAATCGAG AAAACTATGG TTATAGAGAT GGAATCGGCA
    GGAGAGGTGA AGCGGGCTGG GATCCTTTTC TTCCGAACCA TGCTGCAGGA GCTGCAGGAA
    TGCCTCTGCC TCCTCGATTC CATGGACTTT GTGTCGCTGC TTCTCTATGA GCACAAATAT
    CATGTCGACT CCATCCTTCA GGACATCACT CTCCAGGAGG TGGAAGCCAA TGTGGCCCTG
    GTGCACGACG TCCTGAAAGG AGTTATTCTG TTCTCTCAGC GGGAGAAAGA CTCTTTAAGC
    GACTTGGAAG AGTGGGAAAA ATGGAAGTTT CATATTAACA AGTACTTAGT GGAGGAGATT
    TGTGTGCTTG ATCCGACTGC TAGCAACGTG GAGGTTAATG TAGAGCTCGT TACAAGCTAT
    ATCCAAGCTC ATTCTCGGAC TGAAGTCTGG AATTTCAGGA ACATTGTCAT TGAACTCCTG
    TATCACTGGA TAAACATCTG TCTGACTCTC ATAGAGCTAA ACATGCGACA AGATGTTTCC
    ATTATCCCGC CATTGCCCAA GAAGAGTGCC ACCTCTATTT ATGCAATATC CTCAGAAAGA
    TCAATCCGAG AGAAACTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_795939.2
    CDS51..4271
    Translation

    Target ORF information:

    RefSeq Version NM_176965.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176965.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCTGATGC AGGTGGAGAA GAAGAAGCTT TTGAGCCAAG CTGACATCCC AACCAAGTTT 
    GATCCAATTA ATTATTTGGG GGAATATTTA ATGAGACACA ACACTCATTA TATCAAAGAC
    CCAGGAGTGT CTGGCTACCA GAGGGTGATG AAAGAGATCA CTGAAGAACT GAAGACTCAT
    GTCCCTGATA CGATCAACAA CAGAATATCC AAGATGAAGG AGAAAGTGAA AGAGAAGAGA
    GAACAAAGAG AGTACATAAG CACAGTCAAA GTCAAGGTGG CCGGCATGAG AAAGCAGGCT
    CTGGAAGAGC AGTTCAATGA ATGGGTTCTC AACCCCAAAG GGATGATTCC AATAGTCGTG
    TACATTTCTT CATACATCAC AGACTTGAAA AGCGAAGTCT TTGAGGAATT CCTTAAACAC
    CTGTGCCACA GCGCAGAAGA ATTCCGGGAG ATCATACTGA CTGACATGCG GAGGCAGATG
    TTTGCAGAAC TCTTCCTACA GTGCGACAGT GGGAAGGTGA TGGCCTTGGA TCGGCAGAGG
    ACACTGGCCC TGCTGGAAGC GTTCTATGAT CAATGCTCTT CCACCACTAG GAGTTTACTC
    CGAAACCCAC GACAATGGCC CTTTGTTGAA TTTGAAGAGA TAGAGTTGAT TGAGTTCTGG
    GGAGACATGG ATATTAAGAA GCACATTTAT GAAGACTTTG ATGCACTGCT CTTAAAGATG
    AATATGTTAG TTGCTGAAAA ACTTGCAGGC AAACTCGCAG AAAACAAAGA TCTCCCACAG
    CAGCAGAGAG ATCAAGAACT TTCATCTGAC TCAACAACGG AGCCAGAAAC TGCCACACAA
    CTGACCTCAC AACAGAGATC CCGCAGAGTA TCACTTACAG GACAAGGACA GGGCAAAGGG
    CCTCGCAAAA CATCTGCTTC AAAACAAGGA GCAAGCAGAG GGTCAGTGGC AGAGCAAGGG
    TCCCGAAGAA GCTCGGGAGT GAACCAAACC CAGCAGAGAG GGTCGGTGGC AGAACAAGGA
    TCCCGAAGAA GCTCAGCAGT AGAACAAACC CAGCAGAGAG GGTCGGTGGC AGAGCAAGGG
    TCCCGAAGAA GCTCTACAGT AGAACAAACT CGGCAGAGAG GGTCAGTGGC AGAGCAAGGG
    TCCCGAAGAA GCTCTACAGT AGAACAAACC CAGCGGAGAG GGTCGGTGGC AGAGCAAGGG
    TCCCGAAGAA GCTCGGGAGT GAACCAAACC CAGCAGAGAG GGTCGGTGGC AGAACAAGGA
    TCCCGAAGAA GCTCGGCAGT AGAACAAACT CAGCAGAGAG GGTCGGTGGC AGAGCAAGGG
    TCCCGAAGAA GTTCAGCCAT GGAACAAGAG CCACAAACAG CACAAGATCC AAACAGCGAT
    TCATTGCCAG AACAAGAATC ACACAGAGGG TCCATCACAG AAGGATCACA CAGAGGGTCA
    ATTTCAGAAC CTGGGCAACG CAGAGCATCA GTAACAGGAC AACGCAGAAA GTCGTCTGTA
    GATGACTCCG GTAGTGCAGG GTCACGCAGA GGATCAGGGA CAGATCAAGG ACAGCACAGA
    GGATCCGTAG GGCAGCGCAA AGGGTCAGGA GAACGCAAAA TGTCAGCTTC AGAGTATGGA
    CCACATCAAG AGTCCATAAC AGAAGAGCCC CTCACTGCGT CAGAACCAGG ACCGCAAATA
    GACACTATTC AAGAACTGGA TGAAGACTCA ACTCCACAAT TAGAAGACGA TAGTGCCCTA
    AAAGAAAGTA AAACCTCGGA ACTCACTAAA ATAGAAACTC AAGAAGAAAA GCCTTTGCTG
    CTCATCAACG AAGAGCAAGT CCCCAGAGAT TCCAAACAGC CAGAAGTTCC CACATCCAGC
    AAAAAGGAAC GTCCATCAGG CAGCCCCAAA AAAGGGCGCC TTTCAGGAAC TTCAAAAAGG
    GATTCTCAGA AAGACAAAGC CTGTGAACCA AAGCCGCAAC ATGTAGAAGG AAAAAAATGG
    TCAGGAGAAT TTTTGATCTG TGACTGGAAG ATAAAGCATG TCAAATCTGA AGAGGAGGAA
    CAGGCCAAGT TAATCTGTGA TGACACCAGA TTCACAGACC TACATGCGAC TATTAGGAAT
    TTTCAGACTT ACAAGGGGAT CAAAGGGAGG AGTGCCTTCA ATGGAGTCTC CCTCGATTTG
    CTGCAGTTTG TACAGCTCCT GGAGACATTT GTTGGTGAAG ACACCTCCTT CAGTCTCAGC
    AAGGACCTCG CCTCCTTTTT TCAGAAGAAC TATTCTGAAA CAAAGCAAGA GAAAATTAAA
    GCCTTAGAAC AGGCCAGACA AAATTCCTCC CGCATCCGAC GGAGAATTCT CCTCCAAGCC
    ATCTTTGAGA AGTGGGACAA TGATGGCTCA GGCTTTCTGG ATCTGAATGA AGTTGATGAT
    CTCCTGTATA CATATAAGGA GGGAATGGAA AGAGAATCTA TGAAGAAAGC TAAACTGCAC
    ATAAATTTTC CAGAGCCAAG TCCCGGTCAC GAAGTTAAAC TGTCTTCAAA GCAATTTCAG
    AGGTACATAG AACTGGTCGT ATCTGAACTC AGGGGCAATG AAGACGAAGT TCTGGAAAGT
    GTTGTGGAAT TCCTGATGGG TTCTTTAGAG AGGAGCCACG TTGAGGGTCT AAGGAACTGT
    GCAAGGCGGA AATGGCTCCA TCAGATTCAG TACGCTGCAG AGACAAGTGG GGTGTCCCTG
    GAGCCGGTGT ACACTGAGAC CTTCAGGGTC CTCACCCAGG ACGCTGAGGC TCACGGAAAT
    AAGAAGATCA GTGCTCACAT CTCCCTCCTA GAGGAAAACG TGTTCCTGCC TGAGAGAGGG
    CACGTTCTCT TGAGGAACGT GGCTTGTACT CTAGATGACG CTCCGTTCGT CCTGAACAAA
    GTGCTCTACA GGGACATGAA GGGAATCAGC TTTACAGTAG TGGATGAAGG AAAGCCAATC
    CATGTCCCCC AAGTTCAGCA CCATGGAAAT ATCTTCTTCT GGAACAGTTT CCGAAGTAAG
    AATGAGTATA ACGGGTCGTT CCTGGCTTTG CCTCTTCAGG ATGCATACAT GAGGATCTTT
    GGGGTCCTGG CAGTTGACAC GCTTCGAGAT CCTCATGAAA TAAACATCTT CTTACCTCAT
    GAGATCAAAT TCTATCAGGG GGTCGCCAAT GCCTTCAGCA CAGCTTACCA CCATGTTCAC
    AGCAGGGAAC ACGTGCTGCA CTCCGTGATG ACTGGCATCC GGTGGCTCTT CAGTGTCACA
    TCTGGCATCA CCACCATCAC TACATGCTTC ATCGAACCCA GCTCAGAACA GGAAGACTAT
    GTTTTGCGCA ATATGATGGT TACAGATTGT CTCGGTCTAG CAGAAATCCA CACAGATCCC
    CCGACCATCA CGAGGAACGC ATGCATCTTT AGGGACTTCC TCTTTAAATG CACAGACACT
    TCAGAAGTTA TTTTGGCTTC TTCCGGTGGA GAAACCCACA TTGCCATTCC ACTTCGCCAG
    AGAACCAAAG AGGCTATGGG CATCCTCGAT GTTAACATTG GCCGGAGCAG GATGCTGCTG
    TACCAAGAGT ATAAAGATCT ACAGAAAATG GTGAAGATGA TCCAAAACGT CTCCTATGAA
    ATACTGGGCG AATTCTCTGG CGAAATCGAG AAAACTATGG TTATAGAGAT GGAATCGGCA
    GGAGAGGTGA AGCGGGCTGG GATCCTTTTC TTCCGAACCA TGCTGCAGGA GCTGCAGGAA
    TGCCTCTGCC TCCTCGATTC CATGGACTTT GTGTCGCTGC TTCTCTATGA GCACAAATAT
    CATGTCGACT CCATCCTTCA GGACATCACT CTCCAGGAGG TGGAAGCCAA TGTGGCCCTG
    GTGCACGACG TCCTGAAAGG AGTTATTCTG TTCTCTCAGC GGGAGAAAGA CTCTTTAAGC
    GACTTGGAAG AGTGGGAAAA ATGGAAGTTT CATATTAACA AGTACTTAGT GGAGGAGATT
    TGTGTGCTTG ATCCGACTGC TAGCAACGTG GAGGTTAATG TAGAGCTCGT TACAAGCTAT
    ATCCAAGCTC ATTCTCGGAC TGAAGTCTGG AATTTCAGGA ACATTGTCAT TGAACTCCTG
    TATCACTGGA TAAACATCTG TCTGACTCTC ATAGAGCTAA ACATGCGACA AGATGTTTCC
    ATTATCCCGC CATTGCCCAA GAAGAGTGCC ACCTCTATTT ATGCAATATC CTCAGAAAGA
    TCAATCCGAG AGAAACTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu46451
    Clone ID Related Accession (Same CDS sequence) XM_030246082.1 , XM_030246081.1 , XM_006533522.1
    Accession Version XM_006533522.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT 
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006533585.1
    CDS109..4800
    Translation

    Target ORF information:

    RefSeq Version XM_006533522.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006533522.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT 
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GATTCAGAAT
    GTCCTTTATG ATTTTTTTCA GAAGCCAGAG CTGCATCTTG AATCACGTTG CAAAGATCTG
    GTTATTATGA AATCGGTGGA TCTAGGATTG AACCAACAGC AGTTTGTACA ATACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu46452
    Clone ID Related Accession (Same CDS sequence) XM_017314596.1 , XM_017314597.1
    Accession Version XM_017314596.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4572bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT 
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170085.1
    CDS4037..8608
    Translation

    Target ORF information:

    RefSeq Version XM_017314596.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314596.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT 
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu46452
    Clone ID Related Accession (Same CDS sequence) XM_017314596.1 , XM_017314597.1
    Accession Version XM_017314597.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4572bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT 
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170086.1
    CDS3941..8512
    Translation

    Target ORF information:

    RefSeq Version XM_017314597.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314597.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT 
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu46455
    Clone ID Related Accession (Same CDS sequence) XM_017314600.1 , XM_017314600.2
    Accession Version XM_017314600.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCTTTA TGATTTTTTT CAGAAGCCAG AGCTGCATCT TGTACATTTC TTCATACATC 
    ACAGACTTGA AAAGCGAAGT CTTTGAGGAA TTCCTTAAAC ACCTGTGCCA CAGCGCAGAA
    GAATTCCGGG AGATCATACT GACTGACATG CGGAGGCAGA TGTTTGCAGA ACTCTTCCTA
    CAGTGCGACA GTGGGAAGGT GATGGCCTTG GATCGGCAGA GGACACTGGC CCTGCTGGAA
    GCGTTCTATG ATCAATGCTC TTCCACCACT AGGAGTTTAC TCCGAAACCC ACGACAATGG
    CCCTTTGTTG AATTTGAAGA GATAGAGTTG ATTGAGTTCT GGGGAGACAT GGATATTAAG
    AAGCACATTT ATGAAGACTT TGATGCACTG CTCTTAAAGA TGAATATGTT AGTTGCTGAA
    AAACTTGCAG GCAAACTCGC AGAAAACAAA GATCTCCCAC AGCAGCAGAG AGATCAAGAA
    CTTTCATCTG ACTCAACAAC GGAGCCAGAA ACTGCCACAC AACTGACCTC ACAACAGAGA
    TCCCGCAGAG TATCACTTAC AGGACAAGGA CAGGGCAAAG GGCCTCGCAA AACATCTGCT
    TCAAAACAAG GAGCAAGCAG AGGGTCAGTG GCAGAGCAAG GGTCCCGAAG AAGCTCGGGA
    GTGAACCAAA CCCAGCAGAG AGGGTCGGTG GCAGAACAAG GATCCCGAAG AAGCTCAGCA
    GTAGAACAAA CCCAGCAGAG AGGGTCGGTG GCAGAGCAAG GGTCCCGAAG AAGCTCTACA
    GTAGAACAAA CTCGGCAGAG AGGGTCAGTG GCAGAGCAAG GGTCCCGAAG AAGCTCTACA
    GTAGAACAAA CCCAGCGGAG AGGGTCGGTG GCAGAGCAAG GGTCCCGAAG AAGCTCGGGA
    GTGAACCAAA CCCAGCAGAG AGGGTCGGTG GCAGAACAAG GATCCCGAAG AAGCTCGGCA
    GTAGAACAAA CTCAGCAGAG AGGGTCGGTG GCAGAGCAAG GGTCCCGAAG AAGTTCAGCC
    ATGGAACAAG AGCCACAAAC AGCACAAGAT CCAAACAGCG ATTCATTGCC AGAACAAGAA
    TCACACAGAG GGTCCATCAC AGAAGGATCA CACAGAGGGT CAATTTCAGA ACCTGGGCAA
    CGCAGAGCAT CAGTAACAGG ACAACGCAGA AAGTCGTCTG TAGATGACTC CGGTAGTGCA
    GGGTCACGCA GAGGATCAGG GACAGATCAA GGACAGCACA GAGGATCCGT AGGGCAGCGC
    AAAGGGTCAG GAGAACGCAA AATGTCAGCT TCAGAGTATG GACCACATCA AGAGTCCATA
    ACAGAAGAGC CCCTCACTGC GTCAGAACCA GGACCGCAAA TAGACACTAT TCAAGAACTG
    GATGAAGACT CAACTCCACA ATTAGAAGAC GATAGTGCCC TAAAAGAAAG TAAAACCTCG
    GAACTCACTA AAATAGAAAC TCAAGAAGAA AAGCCTTTGC TGCTCATCAA CGAAGAGCAA
    GTCCCCAGAG ATTCCAAACA GCCAGAAGTT CCCACATCCA GCAAAAAGGA ACGTCCATCA
    GGCAGCCCCA AAAAAGGGCG CCTTTCAGGA ACTTCAAAAA GGGATTCTCA GAAAGACAAA
    GCCTGTGAAC CAAAGCCGCA ACATGTAGAA GGAAAAAAAT GGTCAGGAGA ATTTTTGATC
    TGTGACTGGA AGATAAAGCA TGTCAAATCT GAAGAGGAGG AACAGGCCAA GTTAATCTGT
    GATGACACCA GATTCACAGA CCTACATGCG ACTATTAGGA ATTTTCAGAC TTACAAGGGG
    ATCAAAGGGA GGAGTGCCTT CAATGGAGTC TCCCTCGATT TGCTGCAGTT TGTACAGCTC
    CTGGAGACAT TTGTTGGTGA AGACACCTCC TTCAGTCTCA GCAAGGACCT CGCCTCCTTT
    TTTCAGAAGA ACTATTCTGA AACAAAGCAA GAGAAAATTA AAGCCTTAGA ACAGGCCAGA
    CAAAATTCCT CCCGCATCCG ACGGAGAATT CTCCTCCAAG CCATCTTTGA GAAGTGGGAC
    AATGATGGCT CAGGCTTTCT GGATCTGAAT GAAGTTGATG ATCTCCTGTA TACATATAAG
    GAGGGAATGG AAAGAGAATC TATGAAGAAA GCTAAACTGC ACATAAATTT TCCAGAGCCA
    AGTCCCGGTC ACGAAGTTAA ACTGTCTTCA AAGCAATTTC AGAGGTACAT AGAACTGGTC
    GTATCTGAAC TCAGGGGCAA TGAAGACGAA GTTCTGGAAA GTGTTGTGGA ATTCCTGATG
    GGTTCTTTAG AGAGGAGCCA CGTTGAGGGT CTAAGGAACT GTGCAAGGCG GAAATGGCTC
    CATCAGATTC AGTACGCTGC AGAGACAAGT GGGGTGTCCC TGGAGCCGGT GTACACTGAG
    ACCTTCAGGG TCCTCACCCA GGACGCTGAG GCTCACGGAA ATAAGAAGAT CAGTGCTCAC
    ATCTCCCTCC TAGAGGAAAA CGTGTTCCTG CCTGAGAGAG GGCACGTTCT CTTGAGGAAC
    GTGGCTTGTA CTCTAGATGA CGCTCCGTTC GTCCTGAACA AAGTGCTCTA CAGGGACATG
    AAGGGAATCA GCTTTACAGT AGTGGATGAA GGAAAGCCAA TCCATGTCCC CCAAGTTCAG
    CACCATGGAA ATATCTTCTT CTGGAACAGT TTCCGAAGTA AGAATGAGTA TAACGGGTCG
    TTCCTGGCTT TGCCTCTTCA GGATGCATAC ATGAGGATCT TTGGGGTCCT GGCAGTTGAC
    ACGCTTCGAG ATCCTCATGA AATAAACATC TTCTTACCTC ATGAGATCAA ATTCTATCAG
    GGGGTCGCCA ATGCCTTCAG CACAGCTTAC CACCATGTTC ACAGCAGGGA ACACGTGCTG
    CACTCCGTGA TGACTGGCAT CCGGTGGCTC TTCAGTGTCA CATCTGGCAT CACCACCATC
    ACTACATGCT TCATCGAACC CAGCTCAGAA CAGGAAGACT ATGTTTTGCG CAATATGATG
    GTTACAGATT GTCTCGGTCT AGCAGAAATC CACACAGATC CCCCGACCAT CACGAGGAAC
    GCATGCATCT TTAGGGACTT CCTCTTTAAA TGCACAGACA CTTCAGAAGT TATTTTGGCT
    TCTTCCGGTG GAGAAACCCA CATTGCCATT CCACTTCGCC AGAGAACCAA AGAGGCTATG
    GGCATCCTCG ATGTTAACAT TGGCCGGAGC AGGATGCTGC TGTACCAAGA GTATAAAGAT
    CTACAGAAAA TGGTGAAGAT GATCCAAAAC GTCTCCTATG AAATACTGGG CGAATTCTCT
    GGCGAAATCG AGAAAACTAT GGTTATAGAG ATGGAATCGG CAGGAGAGGT GAAGCGGGCT
    GGGATCCTTT TCTTCCGAAC CATGCTGCAG GAGCTGCAGG AATGCCTCTG CCTCCTCGAT
    TCCATGGACT TTGTGTCGCT GCTTCTCTAT GAGCACAAAT ATCATGTCGA CTCCATCCTT
    CAGGACATCA CTCTCCAGGA GGTGGAAGCC AATGTGGCCC TGGTGCACGA CGTCCTGAAA
    GGAGTTATTC TGTTCTCTCA GCGGGAGAAA GACTCTTTAA GCGACTTGGA AGAGTGGGAA
    AAATGGAAGT TTCATATTAA CAAGTACTTA GTGGAGGAGA TTTGTGTGCT TGATCCGACT
    GCTAGCAACG TGGAGGTTAA TGTAGAGCTC GTTACAAGCT ATATCCAAGC TCATTCTCGG
    ACTGAAGTCT GGAATTTCAG GAACATTGTC ATTGAACTCC TGTATCACTG GATAAACATC
    TGTCTGACTC TCATAGAGCT AAACATGCGA CAAGATGTTT CCATTATCCC GCCATTGCCC
    AAGAAGAGTG CCACCTCTAT TTATGCAATA TCCTCAGAAA GATCAATCCG AGAGAAACTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170089.1
    CDS4755..8657
    Translation

    Target ORF information:

    RefSeq Version XM_017314600.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314600.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCTTTA TGATTTTTTT CAGAAGCCAG AGCTGCATCT TGTACATTTC TTCATACATC 
    ACAGACTTGA AAAGCGAAGT CTTTGAGGAA TTCCTTAAAC ACCTGTGCCA CAGCGCAGAA
    GAATTCCGGG AGATCATACT GACTGACATG CGGAGGCAGA TGTTTGCAGA ACTCTTCCTA
    CAGTGCGACA GTGGGAAGGT GATGGCCTTG GATCGGCAGA GGACACTGGC CCTGCTGGAA
    GCGTTCTATG ATCAATGCTC TTCCACCACT AGGAGTTTAC TCCGAAACCC ACGACAATGG
    CCCTTTGTTG AATTTGAAGA GATAGAGTTG ATTGAGTTCT GGGGAGACAT GGATATTAAG
    AAGCACATTT ATGAAGACTT TGATGCACTG CTCTTAAAGA TGAATATGTT AGTTGCTGAA
    AAACTTGCAG GCAAACTCGC AGAAAACAAA GATCTCCCAC AGCAGCAGAG AGATCAAGAA
    CTTTCATCTG ACTCAACAAC GGAGCCAGAA ACTGCCACAC AACTGACCTC ACAACAGAGA
    TCCCGCAGAG TATCACTTAC AGGACAAGGA CAGGGCAAAG GGCCTCGCAA AACATCTGCT
    TCAAAACAAG GAGCAAGCAG AGGGTCAGTG GCAGAGCAAG GGTCCCGAAG AAGCTCGGGA
    GTGAACCAAA CCCAGCAGAG AGGGTCGGTG GCAGAACAAG GATCCCGAAG AAGCTCAGCA
    GTAGAACAAA CCCAGCAGAG AGGGTCGGTG GCAGAGCAAG GGTCCCGAAG AAGCTCTACA
    GTAGAACAAA CTCGGCAGAG AGGGTCAGTG GCAGAGCAAG GGTCCCGAAG AAGCTCTACA
    GTAGAACAAA CCCAGCGGAG AGGGTCGGTG GCAGAGCAAG GGTCCCGAAG AAGCTCGGGA
    GTGAACCAAA CCCAGCAGAG AGGGTCGGTG GCAGAACAAG GATCCCGAAG AAGCTCGGCA
    GTAGAACAAA CTCAGCAGAG AGGGTCGGTG GCAGAGCAAG GGTCCCGAAG AAGTTCAGCC
    ATGGAACAAG AGCCACAAAC AGCACAAGAT CCAAACAGCG ATTCATTGCC AGAACAAGAA
    TCACACAGAG GGTCCATCAC AGAAGGATCA CACAGAGGGT CAATTTCAGA ACCTGGGCAA
    CGCAGAGCAT CAGTAACAGG ACAACGCAGA AAGTCGTCTG TAGATGACTC CGGTAGTGCA
    GGGTCACGCA GAGGATCAGG GACAGATCAA GGACAGCACA GAGGATCCGT AGGGCAGCGC
    AAAGGGTCAG GAGAACGCAA AATGTCAGCT TCAGAGTATG GACCACATCA AGAGTCCATA
    ACAGAAGAGC CCCTCACTGC GTCAGAACCA GGACCGCAAA TAGACACTAT TCAAGAACTG
    GATGAAGACT CAACTCCACA ATTAGAAGAC GATAGTGCCC TAAAAGAAAG TAAAACCTCG
    GAACTCACTA AAATAGAAAC TCAAGAAGAA AAGCCTTTGC TGCTCATCAA CGAAGAGCAA
    GTCCCCAGAG ATTCCAAACA GCCAGAAGTT CCCACATCCA GCAAAAAGGA ACGTCCATCA
    GGCAGCCCCA AAAAAGGGCG CCTTTCAGGA ACTTCAAAAA GGGATTCTCA GAAAGACAAA
    GCCTGTGAAC CAAAGCCGCA ACATGTAGAA GGAAAAAAAT GGTCAGGAGA ATTTTTGATC
    TGTGACTGGA AGATAAAGCA TGTCAAATCT GAAGAGGAGG AACAGGCCAA GTTAATCTGT
    GATGACACCA GATTCACAGA CCTACATGCG ACTATTAGGA ATTTTCAGAC TTACAAGGGG
    ATCAAAGGGA GGAGTGCCTT CAATGGAGTC TCCCTCGATT TGCTGCAGTT TGTACAGCTC
    CTGGAGACAT TTGTTGGTGA AGACACCTCC TTCAGTCTCA GCAAGGACCT CGCCTCCTTT
    TTTCAGAAGA ACTATTCTGA AACAAAGCAA GAGAAAATTA AAGCCTTAGA ACAGGCCAGA
    CAAAATTCCT CCCGCATCCG ACGGAGAATT CTCCTCCAAG CCATCTTTGA GAAGTGGGAC
    AATGATGGCT CAGGCTTTCT GGATCTGAAT GAAGTTGATG ATCTCCTGTA TACATATAAG
    GAGGGAATGG AAAGAGAATC TATGAAGAAA GCTAAACTGC ACATAAATTT TCCAGAGCCA
    AGTCCCGGTC ACGAAGTTAA ACTGTCTTCA AAGCAATTTC AGAGGTACAT AGAACTGGTC
    GTATCTGAAC TCAGGGGCAA TGAAGACGAA GTTCTGGAAA GTGTTGTGGA ATTCCTGATG
    GGTTCTTTAG AGAGGAGCCA CGTTGAGGGT CTAAGGAACT GTGCAAGGCG GAAATGGCTC
    CATCAGATTC AGTACGCTGC AGAGACAAGT GGGGTGTCCC TGGAGCCGGT GTACACTGAG
    ACCTTCAGGG TCCTCACCCA GGACGCTGAG GCTCACGGAA ATAAGAAGAT CAGTGCTCAC
    ATCTCCCTCC TAGAGGAAAA CGTGTTCCTG CCTGAGAGAG GGCACGTTCT CTTGAGGAAC
    GTGGCTTGTA CTCTAGATGA CGCTCCGTTC GTCCTGAACA AAGTGCTCTA CAGGGACATG
    AAGGGAATCA GCTTTACAGT AGTGGATGAA GGAAAGCCAA TCCATGTCCC CCAAGTTCAG
    CACCATGGAA ATATCTTCTT CTGGAACAGT TTCCGAAGTA AGAATGAGTA TAACGGGTCG
    TTCCTGGCTT TGCCTCTTCA GGATGCATAC ATGAGGATCT TTGGGGTCCT GGCAGTTGAC
    ACGCTTCGAG ATCCTCATGA AATAAACATC TTCTTACCTC ATGAGATCAA ATTCTATCAG
    GGGGTCGCCA ATGCCTTCAG CACAGCTTAC CACCATGTTC ACAGCAGGGA ACACGTGCTG
    CACTCCGTGA TGACTGGCAT CCGGTGGCTC TTCAGTGTCA CATCTGGCAT CACCACCATC
    ACTACATGCT TCATCGAACC CAGCTCAGAA CAGGAAGACT ATGTTTTGCG CAATATGATG
    GTTACAGATT GTCTCGGTCT AGCAGAAATC CACACAGATC CCCCGACCAT CACGAGGAAC
    GCATGCATCT TTAGGGACTT CCTCTTTAAA TGCACAGACA CTTCAGAAGT TATTTTGGCT
    TCTTCCGGTG GAGAAACCCA CATTGCCATT CCACTTCGCC AGAGAACCAA AGAGGCTATG
    GGCATCCTCG ATGTTAACAT TGGCCGGAGC AGGATGCTGC TGTACCAAGA GTATAAAGAT
    CTACAGAAAA TGGTGAAGAT GATCCAAAAC GTCTCCTATG AAATACTGGG CGAATTCTCT
    GGCGAAATCG AGAAAACTAT GGTTATAGAG ATGGAATCGG CAGGAGAGGT GAAGCGGGCT
    GGGATCCTTT TCTTCCGAAC CATGCTGCAG GAGCTGCAGG AATGCCTCTG CCTCCTCGAT
    TCCATGGACT TTGTGTCGCT GCTTCTCTAT GAGCACAAAT ATCATGTCGA CTCCATCCTT
    CAGGACATCA CTCTCCAGGA GGTGGAAGCC AATGTGGCCC TGGTGCACGA CGTCCTGAAA
    GGAGTTATTC TGTTCTCTCA GCGGGAGAAA GACTCTTTAA GCGACTTGGA AGAGTGGGAA
    AAATGGAAGT TTCATATTAA CAAGTACTTA GTGGAGGAGA TTTGTGTGCT TGATCCGACT
    GCTAGCAACG TGGAGGTTAA TGTAGAGCTC GTTACAAGCT ATATCCAAGC TCATTCTCGG
    ACTGAAGTCT GGAATTTCAG GAACATTGTC ATTGAACTCC TGTATCACTG GATAAACATC
    TGTCTGACTC TCATAGAGCT AAACATGCGA CAAGATGTTT CCATTATCCC GCCATTGCCC
    AAGAAGAGTG CCACCTCTAT TTATGCAATA TCCTCAGAAA GATCAATCCG AGAGAAACTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77938
    Clone ID Related Accession (Same CDS sequence) XM_017314592.2 , XM_017314592.1
    Accession Version XM_017314592.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170081.1
    CDS441..5372
    Translation

    Target ORF information:

    RefSeq Version XM_017314592.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314592.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77939
    Clone ID Related Accession (Same CDS sequence) XM_017314593.1
    Accession Version XM_017314593.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170082.1
    CDS441..5132
    Translation

    Target ORF information:

    RefSeq Version XM_017314593.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314593.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGAGA TGGAATCGGC AGGAGAGGTG
    AAGCGGGCTG GGATCCTTTT CTTCCGAACC ATGCTGCAGG AGCTGCAGGA ATGCCTCTGC
    CTCCTCGATT CCATGGACTT TGTGTCGCTG CTTCTCTATG AGCACAAATA TCATGTCGAC
    TCCATCCTTC AGGACATCAC TCTCCAGGAG GTGGAAGCCA ATGTGGCCCT GGTGCACGAC
    GTCCTGAAAG GAGTTATTCT GTTCTCTCAG CGGGAGAAAG ACTCTTTAAG CGACTTGGAA
    GAGTGGGAAA AATGGAAGTT TCATATTAAC AAGTACTTAG TGGAGGAGAT TTGTGTGCTT
    GATCCGACTG CTAGCAACGT GGAGGTTAAT GTAGAGCTCG TTACAAGCTA TATCCAAGCT
    CATTCTCGGA CTGAAGTCTG GAATTTCAGG AACATTGTCA TTGAACTCCT GTATCACTGG
    ATAAACATCT GTCTGACTCT CATAGAGCTA AACATGCGAC AAGATGTTTC CATTATCCCG
    CCATTGCCCA AGAAGAGTGC CACCTCTATT TATGCAATAT CCTCAGAAAG ATCAATCCGA
    GAGAAACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77940
    Clone ID Related Accession (Same CDS sequence) XM_017314594.1 , XM_017314595.1 , XM_017314595.2
    Accession Version XM_017314594.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTTAAAA AGCCTGCAAA AGTGATTCTT GGTATAGACG AAAGTGATCT GAGGTTCAAA 
    GTACAGCTGC CGTGGAAGAA AAATCTTAAT TTGAGGATAG AGGAAAGAGC CCAGGCCAAG
    CAACAGAAAA TATTAGAGAA CGATAAACTG AAGAAGGAAC AGGAAAAGAA GGTTACAAAA
    AAGCTCCCTA GGGATGACTT GGCTAAAGAG TGGTTCAATC CTGAAAGCAT GACACTGAAT
    ACCCGGGCGT ACCTGCTGGA CAAGCTTCTA CCCACCTTGG TTCCCGGAGT GGAGCAAATG
    CTGATGCAGG TGGAGAAGAA GAAGCTTTTG AGCCAAGCTG ACATCCCAAC CAAGTTTGAT
    CCAATTAATT ATTTGGGGGA ATATTTAATG AGACACAACA CTCATTATAT CAAAGACCCA
    GGAGTGTCTG GCTACCAGAG GGTGATGAAA GAGATCACTG AAGAACTGAA GACTCATGTC
    CCTGATACGA TCAACAACAG AATATCCAAG ATGAAGGAGA AAGTGAAAGA GAAGAGAGAA
    CAAAGAGAGT ACATAAGCAC AGTCAAAGTC AAGGTGGCCG GCATGAGAAA GCAGGCTCTG
    GAAGAGCAGT TCAATGAATG GGTTCTCAAC CCCAAAGGGA TGATTCCAAT AGTCGTGATT
    CAGAATGTCC TTTATGATTT TTTTCAGAAG CCAGAGCTGC ATCTTGAATC ACGTTGCAAA
    GATCTGGTTA TTATGAAATC GGTGGATCTA GGATTGAACC AACAGCAGTT TGTACAATAC
    ATTTCTTCAT ACATCACAGA CTTGAAAAGC GAAGTCTTTG AGGAATTCCT TAAACACCTG
    TGCCACAGCG CAGAAGAATT CCGGGAGATC ATACTGACTG ACATGCGGAG GCAGATGTTT
    GCAGAACTCT TCCTACAGTG CGACAGTGGG AAGGTGATGG CCTTGGATCG GCAGAGGACA
    CTGGCCCTGC TGGAAGCGTT CTATGATCAA TGCTCTTCCA CCACTAGGAG TTTACTCCGA
    AACCCACGAC AATGGCCCTT TGTTGAATTT GAAGAGATAG AGTTGATTGA GTTCTGGGGA
    GACATGGATA TTAAGAAGCA CATTTATGAA GACTTTGATG CACTGCTCTT AAAGATGAAT
    ATGTTAGTTG CTGAAAAACT TGCAGGCAAA CTCGCAGAAA ACAAAGATCT CCCACAGCAG
    CAGAGAGATC AAGAACTTTC ATCTGACTCA ACAACGGAGC CAGAAACTGC CACACAACTG
    ACCTCACAAC AGAGATCCCG CAGAGTATCA CTTACAGGAC AAGGACAGGG CAAAGGGCCT
    CGCAAAACAT CTGCTTCAAA ACAAGGAGCA AGCAGAGGGT CAGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CGGGAGTGAA CCAAACCCAG CAGAGAGGGT CGGTGGCAGA ACAAGGATCC
    CGAAGAAGCT CAGCAGTAGA ACAAACCCAG CAGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CTACAGTAGA ACAAACTCGG CAGAGAGGGT CAGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CTACAGTAGA ACAAACCCAG CGGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CGGGAGTGAA CCAAACCCAG CAGAGAGGGT CGGTGGCAGA ACAAGGATCC
    CGAAGAAGCT CGGCAGTAGA ACAAACTCAG CAGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGTT CAGCCATGGA ACAAGAGCCA CAAACAGCAC AAGATCCAAA CAGCGATTCA
    TTGCCAGAAC AAGAATCACA CAGAGGGTCC ATCACAGAAG GATCACACAG AGGGTCAATT
    TCAGAACCTG GGCAACGCAG AGCATCAGTA ACAGGACAAC GCAGAAAGTC GTCTGTAGAT
    GACTCCGGTA GTGCAGGGTC ACGCAGAGGA TCAGGGACAG ATCAAGGACA GCACAGAGGA
    TCCGTAGGGC AGCGCAAAGG GTCAGGAGAA CGCAAAATGT CAGCTTCAGA GTATGGACCA
    CATCAAGAGT CCATAACAGA AGAGCCCCTC ACTGCGTCAG AACCAGGACC GCAAATAGAC
    ACTATTCAAG AACTGGATGA AGACTCAACT CCACAATTAG AAGACGATAG TGCCCTAAAA
    GAAAGTAAAA CCTCGGAACT CACTAAAATA GAAACTCAAG AAGAAAAGCC TTTGCTGCTC
    ATCAACGAAG AGCAAGTCCC CAGAGATTCC AAACAGCCAG AAGTTCCCAC ATCCAGCAAA
    AAGGAACGTC CATCAGGCAG CCCCAAAAAA GGGCGCCTTT CAGGAACTTC AAAAAGGGAT
    TCTCAGAAAG ACAAAGCCTG TGAACCAAAG CCGCAACATG TAGAAGGAAA AAAATGGTCA
    GGAGAATTTT TGATCTGTGA CTGGAAGATA AAGCATGTCA AATCTGAAGA GGAGGAACAG
    GCCAAGTTAA TCTGTGATGA CACCAGATTC ACAGACCTAC ATGCGACTAT TAGGAATTTT
    CAGACTTACA AGGGGATCAA AGGGAGGAGT GCCTTCAATG GAGTCTCCCT CGATTTGCTG
    CAGTTTGTAC AGCTCCTGGA GACATTTGTT GGTGAAGACA CCTCCTTCAG TCTCAGCAAG
    GACCTCGCCT CCTTTTTTCA GAAGAACTAT TCTGAAACAA AGCAAGAGAA AATTAAAGCC
    TTAGAACAGG CCAGACAAAA TTCCTCCCGC ATCCGACGGA GAATTCTCCT CCAAGCCATC
    TTTGAGAAGT GGGACAATGA TGGCTCAGGC TTTCTGGATC TGAATGAAGT TGATGATCTC
    CTGTATACAT ATAAGGAGGG AATGGAAAGA GAATCTATGA AGAAAGCTAA ACTGCACATA
    AATTTTCCAG AGCCAAGTCC CGGTCACGAA GTTAAACTGT CTTCAAAGCA ATTTCAGAGG
    TACATAGAAC TGGTCGTATC TGAACTCAGG GGCAATGAAG ACGAAGTTCT GGAAAGTGTT
    GTGGAATTCC TGATGGGTTC TTTAGAGAGG AGCCACGTTG AGGGTCTAAG GAACTGTGCA
    AGGCGGAAAT GGCTCCATCA GATTCAGTAC GCTGCAGAGA CAAGTGGGGT GTCCCTGGAG
    CCGGTGTACA CTGAGACCTT CAGGGTCCTC ACCCAGGACG CTGAGGCTCA CGGAAATAAG
    AAGATCAGTG CTCACATCTC CCTCCTAGAG GAAAACGTGT TCCTGCCTGA GAGAGGGCAC
    GTTCTCTTGA GGAACGTGGC TTGTACTCTA GATGACGCTC CGTTCGTCCT GAACAAAGTG
    CTCTACAGGG ACATGAAGGG AATCAGCTTT ACAGTAGTGG ATGAAGGAAA GCCAATCCAT
    GTCCCCCAAG TTCAGCACCA TGGAAATATC TTCTTCTGGA ACAGTTTCCG AAGTAAGAAT
    GAGTATAACG GGTCGTTCCT GGCTTTGCCT CTTCAGGATG CATACATGAG GATCTTTGGG
    GTCCTGGCAG TTGACACGCT TCGAGATCCT CATGAAATAA ACATCTTCTT ACCTCATGAG
    ATCAAATTCT ATCAGGGGGT CGCCAATGCC TTCAGCACAG CTTACCACCA TGTTCACAGC
    AGGGAACACG TGCTGCACTC CGTGATGACT GGCATCCGGT GGCTCTTCAG TGTCACATCT
    GGCATCACCA CCATCACTAC ATGCTTCATC GAACCCAGCT CAGAACAGGA AGACTATGTT
    TTGCGCAATA TGATGGTTAC AGATTGTCTC GGTCTAGCAG AAATCCACAC AGATCCCCCG
    ACCATCACGA GGAACGCATG CATCTTTAGG GACTTCCTCT TTAAATGCAC AGACACTTCA
    GAAGTTATTT TGGCTTCTTC CGGTGGAGAA ACCCACATTG CCATTCCACT TCGCCAGAGA
    ACCAAAGAGG CTATGGGCAT CCTCGATGTT AACATTGGCC GGAGCAGGAT GCTGCTGTAC
    CAAGAGTATA AAGATCTACA GAAAATGGTG AAGATGATCC AAAACGTCTC CTATGAAATA
    CTGGGCGAAT TCTCTGGCGA AATCGAGAAA ACTATGGTTA TAGAGATGGA ATCGGCAGGA
    GAGGTGAAGC GGGCTGGGAT CCTTTTCTTC CGAACCATGC TGCAGGAGCT GCAGGAATGC
    CTCTGCCTCC TCGATTCCAT GGACTTTGTG TCGCTGCTTC TCTATGAGCA CAAATATCAT
    GTCGACTCCA TCCTTCAGGA CATCACTCTC CAGGAGGTGG AAGCCAATGT GGCCCTGGTG
    CACGACGTCC TGAAAGGAGT TATTCTGTTC TCTCAGCGGG AGAAAGACTC TTTAAGCGAC
    TTGGAAGAGT GGGAAAAATG GAAGTTTCAT ATTAACAAGT ACTTAGTGGA GGAGATTTGT
    GTGCTTGATC CGACTGCTAG CAACGTGGAG GTTAATGTAG AGCTCGTTAC AAGCTATATC
    CAAGCTCATT CTCGGACTGA AGTCTGGAAT TTCAGGAACA TTGTCATTGA ACTCCTGTAT
    CACTGGATAA ACATCTGTCT GACTCTCATA GAGCTAAACA TGCGACAAGA TGTTTCCATT
    ATCCCGCCAT TGCCCAAGAA GAGTGCCACC TCTATTTATG CAATATCCTC AGAAAGATCA
    ATCCGAGAGA AACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170083.1
    CDS966..5603
    Translation

    Target ORF information:

    RefSeq Version XM_017314594.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314594.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTTAAAA AGCCTGCAAA AGTGATTCTT GGTATAGACG AAAGTGATCT GAGGTTCAAA 
    GTACAGCTGC CGTGGAAGAA AAATCTTAAT TTGAGGATAG AGGAAAGAGC CCAGGCCAAG
    CAACAGAAAA TATTAGAGAA CGATAAACTG AAGAAGGAAC AGGAAAAGAA GGTTACAAAA
    AAGCTCCCTA GGGATGACTT GGCTAAAGAG TGGTTCAATC CTGAAAGCAT GACACTGAAT
    ACCCGGGCGT ACCTGCTGGA CAAGCTTCTA CCCACCTTGG TTCCCGGAGT GGAGCAAATG
    CTGATGCAGG TGGAGAAGAA GAAGCTTTTG AGCCAAGCTG ACATCCCAAC CAAGTTTGAT
    CCAATTAATT ATTTGGGGGA ATATTTAATG AGACACAACA CTCATTATAT CAAAGACCCA
    GGAGTGTCTG GCTACCAGAG GGTGATGAAA GAGATCACTG AAGAACTGAA GACTCATGTC
    CCTGATACGA TCAACAACAG AATATCCAAG ATGAAGGAGA AAGTGAAAGA GAAGAGAGAA
    CAAAGAGAGT ACATAAGCAC AGTCAAAGTC AAGGTGGCCG GCATGAGAAA GCAGGCTCTG
    GAAGAGCAGT TCAATGAATG GGTTCTCAAC CCCAAAGGGA TGATTCCAAT AGTCGTGATT
    CAGAATGTCC TTTATGATTT TTTTCAGAAG CCAGAGCTGC ATCTTGAATC ACGTTGCAAA
    GATCTGGTTA TTATGAAATC GGTGGATCTA GGATTGAACC AACAGCAGTT TGTACAATAC
    ATTTCTTCAT ACATCACAGA CTTGAAAAGC GAAGTCTTTG AGGAATTCCT TAAACACCTG
    TGCCACAGCG CAGAAGAATT CCGGGAGATC ATACTGACTG ACATGCGGAG GCAGATGTTT
    GCAGAACTCT TCCTACAGTG CGACAGTGGG AAGGTGATGG CCTTGGATCG GCAGAGGACA
    CTGGCCCTGC TGGAAGCGTT CTATGATCAA TGCTCTTCCA CCACTAGGAG TTTACTCCGA
    AACCCACGAC AATGGCCCTT TGTTGAATTT GAAGAGATAG AGTTGATTGA GTTCTGGGGA
    GACATGGATA TTAAGAAGCA CATTTATGAA GACTTTGATG CACTGCTCTT AAAGATGAAT
    ATGTTAGTTG CTGAAAAACT TGCAGGCAAA CTCGCAGAAA ACAAAGATCT CCCACAGCAG
    CAGAGAGATC AAGAACTTTC ATCTGACTCA ACAACGGAGC CAGAAACTGC CACACAACTG
    ACCTCACAAC AGAGATCCCG CAGAGTATCA CTTACAGGAC AAGGACAGGG CAAAGGGCCT
    CGCAAAACAT CTGCTTCAAA ACAAGGAGCA AGCAGAGGGT CAGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CGGGAGTGAA CCAAACCCAG CAGAGAGGGT CGGTGGCAGA ACAAGGATCC
    CGAAGAAGCT CAGCAGTAGA ACAAACCCAG CAGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CTACAGTAGA ACAAACTCGG CAGAGAGGGT CAGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CTACAGTAGA ACAAACCCAG CGGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CGGGAGTGAA CCAAACCCAG CAGAGAGGGT CGGTGGCAGA ACAAGGATCC
    CGAAGAAGCT CGGCAGTAGA ACAAACTCAG CAGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGTT CAGCCATGGA ACAAGAGCCA CAAACAGCAC AAGATCCAAA CAGCGATTCA
    TTGCCAGAAC AAGAATCACA CAGAGGGTCC ATCACAGAAG GATCACACAG AGGGTCAATT
    TCAGAACCTG GGCAACGCAG AGCATCAGTA ACAGGACAAC GCAGAAAGTC GTCTGTAGAT
    GACTCCGGTA GTGCAGGGTC ACGCAGAGGA TCAGGGACAG ATCAAGGACA GCACAGAGGA
    TCCGTAGGGC AGCGCAAAGG GTCAGGAGAA CGCAAAATGT CAGCTTCAGA GTATGGACCA
    CATCAAGAGT CCATAACAGA AGAGCCCCTC ACTGCGTCAG AACCAGGACC GCAAATAGAC
    ACTATTCAAG AACTGGATGA AGACTCAACT CCACAATTAG AAGACGATAG TGCCCTAAAA
    GAAAGTAAAA CCTCGGAACT CACTAAAATA GAAACTCAAG AAGAAAAGCC TTTGCTGCTC
    ATCAACGAAG AGCAAGTCCC CAGAGATTCC AAACAGCCAG AAGTTCCCAC ATCCAGCAAA
    AAGGAACGTC CATCAGGCAG CCCCAAAAAA GGGCGCCTTT CAGGAACTTC AAAAAGGGAT
    TCTCAGAAAG ACAAAGCCTG TGAACCAAAG CCGCAACATG TAGAAGGAAA AAAATGGTCA
    GGAGAATTTT TGATCTGTGA CTGGAAGATA AAGCATGTCA AATCTGAAGA GGAGGAACAG
    GCCAAGTTAA TCTGTGATGA CACCAGATTC ACAGACCTAC ATGCGACTAT TAGGAATTTT
    CAGACTTACA AGGGGATCAA AGGGAGGAGT GCCTTCAATG GAGTCTCCCT CGATTTGCTG
    CAGTTTGTAC AGCTCCTGGA GACATTTGTT GGTGAAGACA CCTCCTTCAG TCTCAGCAAG
    GACCTCGCCT CCTTTTTTCA GAAGAACTAT TCTGAAACAA AGCAAGAGAA AATTAAAGCC
    TTAGAACAGG CCAGACAAAA TTCCTCCCGC ATCCGACGGA GAATTCTCCT CCAAGCCATC
    TTTGAGAAGT GGGACAATGA TGGCTCAGGC TTTCTGGATC TGAATGAAGT TGATGATCTC
    CTGTATACAT ATAAGGAGGG AATGGAAAGA GAATCTATGA AGAAAGCTAA ACTGCACATA
    AATTTTCCAG AGCCAAGTCC CGGTCACGAA GTTAAACTGT CTTCAAAGCA ATTTCAGAGG
    TACATAGAAC TGGTCGTATC TGAACTCAGG GGCAATGAAG ACGAAGTTCT GGAAAGTGTT
    GTGGAATTCC TGATGGGTTC TTTAGAGAGG AGCCACGTTG AGGGTCTAAG GAACTGTGCA
    AGGCGGAAAT GGCTCCATCA GATTCAGTAC GCTGCAGAGA CAAGTGGGGT GTCCCTGGAG
    CCGGTGTACA CTGAGACCTT CAGGGTCCTC ACCCAGGACG CTGAGGCTCA CGGAAATAAG
    AAGATCAGTG CTCACATCTC CCTCCTAGAG GAAAACGTGT TCCTGCCTGA GAGAGGGCAC
    GTTCTCTTGA GGAACGTGGC TTGTACTCTA GATGACGCTC CGTTCGTCCT GAACAAAGTG
    CTCTACAGGG ACATGAAGGG AATCAGCTTT ACAGTAGTGG ATGAAGGAAA GCCAATCCAT
    GTCCCCCAAG TTCAGCACCA TGGAAATATC TTCTTCTGGA ACAGTTTCCG AAGTAAGAAT
    GAGTATAACG GGTCGTTCCT GGCTTTGCCT CTTCAGGATG CATACATGAG GATCTTTGGG
    GTCCTGGCAG TTGACACGCT TCGAGATCCT CATGAAATAA ACATCTTCTT ACCTCATGAG
    ATCAAATTCT ATCAGGGGGT CGCCAATGCC TTCAGCACAG CTTACCACCA TGTTCACAGC
    AGGGAACACG TGCTGCACTC CGTGATGACT GGCATCCGGT GGCTCTTCAG TGTCACATCT
    GGCATCACCA CCATCACTAC ATGCTTCATC GAACCCAGCT CAGAACAGGA AGACTATGTT
    TTGCGCAATA TGATGGTTAC AGATTGTCTC GGTCTAGCAG AAATCCACAC AGATCCCCCG
    ACCATCACGA GGAACGCATG CATCTTTAGG GACTTCCTCT TTAAATGCAC AGACACTTCA
    GAAGTTATTT TGGCTTCTTC CGGTGGAGAA ACCCACATTG CCATTCCACT TCGCCAGAGA
    ACCAAAGAGG CTATGGGCAT CCTCGATGTT AACATTGGCC GGAGCAGGAT GCTGCTGTAC
    CAAGAGTATA AAGATCTACA GAAAATGGTG AAGATGATCC AAAACGTCTC CTATGAAATA
    CTGGGCGAAT TCTCTGGCGA AATCGAGAAA ACTATGGTTA TAGAGATGGA ATCGGCAGGA
    GAGGTGAAGC GGGCTGGGAT CCTTTTCTTC CGAACCATGC TGCAGGAGCT GCAGGAATGC
    CTCTGCCTCC TCGATTCCAT GGACTTTGTG TCGCTGCTTC TCTATGAGCA CAAATATCAT
    GTCGACTCCA TCCTTCAGGA CATCACTCTC CAGGAGGTGG AAGCCAATGT GGCCCTGGTG
    CACGACGTCC TGAAAGGAGT TATTCTGTTC TCTCAGCGGG AGAAAGACTC TTTAAGCGAC
    TTGGAAGAGT GGGAAAAATG GAAGTTTCAT ATTAACAAGT ACTTAGTGGA GGAGATTTGT
    GTGCTTGATC CGACTGCTAG CAACGTGGAG GTTAATGTAG AGCTCGTTAC AAGCTATATC
    CAAGCTCATT CTCGGACTGA AGTCTGGAAT TTCAGGAACA TTGTCATTGA ACTCCTGTAT
    CACTGGATAA ACATCTGTCT GACTCTCATA GAGCTAAACA TGCGACAAGA TGTTTCCATT
    ATCCCGCCAT TGCCCAAGAA GAGTGCCACC TCTATTTATG CAATATCCTC AGAAAGATCA
    ATCCGAGAGA AACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77940
    Clone ID Related Accession (Same CDS sequence) XM_017314594.1 , XM_017314595.1 , XM_017314595.2
    Accession Version XM_017314595.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTTAAAA AGCCTGCAAA AGTGATTCTT GGTATAGACG AAAGTGATCT GAGGTTCAAA 
    GTACAGCTGC CGTGGAAGAA AAATCTTAAT TTGAGGATAG AGGAAAGAGC CCAGGCCAAG
    CAACAGAAAA TATTAGAGAA CGATAAACTG AAGAAGGAAC AGGAAAAGAA GGTTACAAAA
    AAGCTCCCTA GGGATGACTT GGCTAAAGAG TGGTTCAATC CTGAAAGCAT GACACTGAAT
    ACCCGGGCGT ACCTGCTGGA CAAGCTTCTA CCCACCTTGG TTCCCGGAGT GGAGCAAATG
    CTGATGCAGG TGGAGAAGAA GAAGCTTTTG AGCCAAGCTG ACATCCCAAC CAAGTTTGAT
    CCAATTAATT ATTTGGGGGA ATATTTAATG AGACACAACA CTCATTATAT CAAAGACCCA
    GGAGTGTCTG GCTACCAGAG GGTGATGAAA GAGATCACTG AAGAACTGAA GACTCATGTC
    CCTGATACGA TCAACAACAG AATATCCAAG ATGAAGGAGA AAGTGAAAGA GAAGAGAGAA
    CAAAGAGAGT ACATAAGCAC AGTCAAAGTC AAGGTGGCCG GCATGAGAAA GCAGGCTCTG
    GAAGAGCAGT TCAATGAATG GGTTCTCAAC CCCAAAGGGA TGATTCCAAT AGTCGTGATT
    CAGAATGTCC TTTATGATTT TTTTCAGAAG CCAGAGCTGC ATCTTGAATC ACGTTGCAAA
    GATCTGGTTA TTATGAAATC GGTGGATCTA GGATTGAACC AACAGCAGTT TGTACAATAC
    ATTTCTTCAT ACATCACAGA CTTGAAAAGC GAAGTCTTTG AGGAATTCCT TAAACACCTG
    TGCCACAGCG CAGAAGAATT CCGGGAGATC ATACTGACTG ACATGCGGAG GCAGATGTTT
    GCAGAACTCT TCCTACAGTG CGACAGTGGG AAGGTGATGG CCTTGGATCG GCAGAGGACA
    CTGGCCCTGC TGGAAGCGTT CTATGATCAA TGCTCTTCCA CCACTAGGAG TTTACTCCGA
    AACCCACGAC AATGGCCCTT TGTTGAATTT GAAGAGATAG AGTTGATTGA GTTCTGGGGA
    GACATGGATA TTAAGAAGCA CATTTATGAA GACTTTGATG CACTGCTCTT AAAGATGAAT
    ATGTTAGTTG CTGAAAAACT TGCAGGCAAA CTCGCAGAAA ACAAAGATCT CCCACAGCAG
    CAGAGAGATC AAGAACTTTC ATCTGACTCA ACAACGGAGC CAGAAACTGC CACACAACTG
    ACCTCACAAC AGAGATCCCG CAGAGTATCA CTTACAGGAC AAGGACAGGG CAAAGGGCCT
    CGCAAAACAT CTGCTTCAAA ACAAGGAGCA AGCAGAGGGT CAGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CGGGAGTGAA CCAAACCCAG CAGAGAGGGT CGGTGGCAGA ACAAGGATCC
    CGAAGAAGCT CAGCAGTAGA ACAAACCCAG CAGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CTACAGTAGA ACAAACTCGG CAGAGAGGGT CAGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CTACAGTAGA ACAAACCCAG CGGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CGGGAGTGAA CCAAACCCAG CAGAGAGGGT CGGTGGCAGA ACAAGGATCC
    CGAAGAAGCT CGGCAGTAGA ACAAACTCAG CAGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGTT CAGCCATGGA ACAAGAGCCA CAAACAGCAC AAGATCCAAA CAGCGATTCA
    TTGCCAGAAC AAGAATCACA CAGAGGGTCC ATCACAGAAG GATCACACAG AGGGTCAATT
    TCAGAACCTG GGCAACGCAG AGCATCAGTA ACAGGACAAC GCAGAAAGTC GTCTGTAGAT
    GACTCCGGTA GTGCAGGGTC ACGCAGAGGA TCAGGGACAG ATCAAGGACA GCACAGAGGA
    TCCGTAGGGC AGCGCAAAGG GTCAGGAGAA CGCAAAATGT CAGCTTCAGA GTATGGACCA
    CATCAAGAGT CCATAACAGA AGAGCCCCTC ACTGCGTCAG AACCAGGACC GCAAATAGAC
    ACTATTCAAG AACTGGATGA AGACTCAACT CCACAATTAG AAGACGATAG TGCCCTAAAA
    GAAAGTAAAA CCTCGGAACT CACTAAAATA GAAACTCAAG AAGAAAAGCC TTTGCTGCTC
    ATCAACGAAG AGCAAGTCCC CAGAGATTCC AAACAGCCAG AAGTTCCCAC ATCCAGCAAA
    AAGGAACGTC CATCAGGCAG CCCCAAAAAA GGGCGCCTTT CAGGAACTTC AAAAAGGGAT
    TCTCAGAAAG ACAAAGCCTG TGAACCAAAG CCGCAACATG TAGAAGGAAA AAAATGGTCA
    GGAGAATTTT TGATCTGTGA CTGGAAGATA AAGCATGTCA AATCTGAAGA GGAGGAACAG
    GCCAAGTTAA TCTGTGATGA CACCAGATTC ACAGACCTAC ATGCGACTAT TAGGAATTTT
    CAGACTTACA AGGGGATCAA AGGGAGGAGT GCCTTCAATG GAGTCTCCCT CGATTTGCTG
    CAGTTTGTAC AGCTCCTGGA GACATTTGTT GGTGAAGACA CCTCCTTCAG TCTCAGCAAG
    GACCTCGCCT CCTTTTTTCA GAAGAACTAT TCTGAAACAA AGCAAGAGAA AATTAAAGCC
    TTAGAACAGG CCAGACAAAA TTCCTCCCGC ATCCGACGGA GAATTCTCCT CCAAGCCATC
    TTTGAGAAGT GGGACAATGA TGGCTCAGGC TTTCTGGATC TGAATGAAGT TGATGATCTC
    CTGTATACAT ATAAGGAGGG AATGGAAAGA GAATCTATGA AGAAAGCTAA ACTGCACATA
    AATTTTCCAG AGCCAAGTCC CGGTCACGAA GTTAAACTGT CTTCAAAGCA ATTTCAGAGG
    TACATAGAAC TGGTCGTATC TGAACTCAGG GGCAATGAAG ACGAAGTTCT GGAAAGTGTT
    GTGGAATTCC TGATGGGTTC TTTAGAGAGG AGCCACGTTG AGGGTCTAAG GAACTGTGCA
    AGGCGGAAAT GGCTCCATCA GATTCAGTAC GCTGCAGAGA CAAGTGGGGT GTCCCTGGAG
    CCGGTGTACA CTGAGACCTT CAGGGTCCTC ACCCAGGACG CTGAGGCTCA CGGAAATAAG
    AAGATCAGTG CTCACATCTC CCTCCTAGAG GAAAACGTGT TCCTGCCTGA GAGAGGGCAC
    GTTCTCTTGA GGAACGTGGC TTGTACTCTA GATGACGCTC CGTTCGTCCT GAACAAAGTG
    CTCTACAGGG ACATGAAGGG AATCAGCTTT ACAGTAGTGG ATGAAGGAAA GCCAATCCAT
    GTCCCCCAAG TTCAGCACCA TGGAAATATC TTCTTCTGGA ACAGTTTCCG AAGTAAGAAT
    GAGTATAACG GGTCGTTCCT GGCTTTGCCT CTTCAGGATG CATACATGAG GATCTTTGGG
    GTCCTGGCAG TTGACACGCT TCGAGATCCT CATGAAATAA ACATCTTCTT ACCTCATGAG
    ATCAAATTCT ATCAGGGGGT CGCCAATGCC TTCAGCACAG CTTACCACCA TGTTCACAGC
    AGGGAACACG TGCTGCACTC CGTGATGACT GGCATCCGGT GGCTCTTCAG TGTCACATCT
    GGCATCACCA CCATCACTAC ATGCTTCATC GAACCCAGCT CAGAACAGGA AGACTATGTT
    TTGCGCAATA TGATGGTTAC AGATTGTCTC GGTCTAGCAG AAATCCACAC AGATCCCCCG
    ACCATCACGA GGAACGCATG CATCTTTAGG GACTTCCTCT TTAAATGCAC AGACACTTCA
    GAAGTTATTT TGGCTTCTTC CGGTGGAGAA ACCCACATTG CCATTCCACT TCGCCAGAGA
    ACCAAAGAGG CTATGGGCAT CCTCGATGTT AACATTGGCC GGAGCAGGAT GCTGCTGTAC
    CAAGAGTATA AAGATCTACA GAAAATGGTG AAGATGATCC AAAACGTCTC CTATGAAATA
    CTGGGCGAAT TCTCTGGCGA AATCGAGAAA ACTATGGTTA TAGAGATGGA ATCGGCAGGA
    GAGGTGAAGC GGGCTGGGAT CCTTTTCTTC CGAACCATGC TGCAGGAGCT GCAGGAATGC
    CTCTGCCTCC TCGATTCCAT GGACTTTGTG TCGCTGCTTC TCTATGAGCA CAAATATCAT
    GTCGACTCCA TCCTTCAGGA CATCACTCTC CAGGAGGTGG AAGCCAATGT GGCCCTGGTG
    CACGACGTCC TGAAAGGAGT TATTCTGTTC TCTCAGCGGG AGAAAGACTC TTTAAGCGAC
    TTGGAAGAGT GGGAAAAATG GAAGTTTCAT ATTAACAAGT ACTTAGTGGA GGAGATTTGT
    GTGCTTGATC CGACTGCTAG CAACGTGGAG GTTAATGTAG AGCTCGTTAC AAGCTATATC
    CAAGCTCATT CTCGGACTGA AGTCTGGAAT TTCAGGAACA TTGTCATTGA ACTCCTGTAT
    CACTGGATAA ACATCTGTCT GACTCTCATA GAGCTAAACA TGCGACAAGA TGTTTCCATT
    ATCCCGCCAT TGCCCAAGAA GAGTGCCACC TCTATTTATG CAATATCCTC AGAAAGATCA
    ATCCGAGAGA AACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170084.1
    CDS1107..5744
    Translation

    Target ORF information:

    RefSeq Version XM_017314595.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314595.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTTAAAA AGCCTGCAAA AGTGATTCTT GGTATAGACG AAAGTGATCT GAGGTTCAAA 
    GTACAGCTGC CGTGGAAGAA AAATCTTAAT TTGAGGATAG AGGAAAGAGC CCAGGCCAAG
    CAACAGAAAA TATTAGAGAA CGATAAACTG AAGAAGGAAC AGGAAAAGAA GGTTACAAAA
    AAGCTCCCTA GGGATGACTT GGCTAAAGAG TGGTTCAATC CTGAAAGCAT GACACTGAAT
    ACCCGGGCGT ACCTGCTGGA CAAGCTTCTA CCCACCTTGG TTCCCGGAGT GGAGCAAATG
    CTGATGCAGG TGGAGAAGAA GAAGCTTTTG AGCCAAGCTG ACATCCCAAC CAAGTTTGAT
    CCAATTAATT ATTTGGGGGA ATATTTAATG AGACACAACA CTCATTATAT CAAAGACCCA
    GGAGTGTCTG GCTACCAGAG GGTGATGAAA GAGATCACTG AAGAACTGAA GACTCATGTC
    CCTGATACGA TCAACAACAG AATATCCAAG ATGAAGGAGA AAGTGAAAGA GAAGAGAGAA
    CAAAGAGAGT ACATAAGCAC AGTCAAAGTC AAGGTGGCCG GCATGAGAAA GCAGGCTCTG
    GAAGAGCAGT TCAATGAATG GGTTCTCAAC CCCAAAGGGA TGATTCCAAT AGTCGTGATT
    CAGAATGTCC TTTATGATTT TTTTCAGAAG CCAGAGCTGC ATCTTGAATC ACGTTGCAAA
    GATCTGGTTA TTATGAAATC GGTGGATCTA GGATTGAACC AACAGCAGTT TGTACAATAC
    ATTTCTTCAT ACATCACAGA CTTGAAAAGC GAAGTCTTTG AGGAATTCCT TAAACACCTG
    TGCCACAGCG CAGAAGAATT CCGGGAGATC ATACTGACTG ACATGCGGAG GCAGATGTTT
    GCAGAACTCT TCCTACAGTG CGACAGTGGG AAGGTGATGG CCTTGGATCG GCAGAGGACA
    CTGGCCCTGC TGGAAGCGTT CTATGATCAA TGCTCTTCCA CCACTAGGAG TTTACTCCGA
    AACCCACGAC AATGGCCCTT TGTTGAATTT GAAGAGATAG AGTTGATTGA GTTCTGGGGA
    GACATGGATA TTAAGAAGCA CATTTATGAA GACTTTGATG CACTGCTCTT AAAGATGAAT
    ATGTTAGTTG CTGAAAAACT TGCAGGCAAA CTCGCAGAAA ACAAAGATCT CCCACAGCAG
    CAGAGAGATC AAGAACTTTC ATCTGACTCA ACAACGGAGC CAGAAACTGC CACACAACTG
    ACCTCACAAC AGAGATCCCG CAGAGTATCA CTTACAGGAC AAGGACAGGG CAAAGGGCCT
    CGCAAAACAT CTGCTTCAAA ACAAGGAGCA AGCAGAGGGT CAGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CGGGAGTGAA CCAAACCCAG CAGAGAGGGT CGGTGGCAGA ACAAGGATCC
    CGAAGAAGCT CAGCAGTAGA ACAAACCCAG CAGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CTACAGTAGA ACAAACTCGG CAGAGAGGGT CAGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CTACAGTAGA ACAAACCCAG CGGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGCT CGGGAGTGAA CCAAACCCAG CAGAGAGGGT CGGTGGCAGA ACAAGGATCC
    CGAAGAAGCT CGGCAGTAGA ACAAACTCAG CAGAGAGGGT CGGTGGCAGA GCAAGGGTCC
    CGAAGAAGTT CAGCCATGGA ACAAGAGCCA CAAACAGCAC AAGATCCAAA CAGCGATTCA
    TTGCCAGAAC AAGAATCACA CAGAGGGTCC ATCACAGAAG GATCACACAG AGGGTCAATT
    TCAGAACCTG GGCAACGCAG AGCATCAGTA ACAGGACAAC GCAGAAAGTC GTCTGTAGAT
    GACTCCGGTA GTGCAGGGTC ACGCAGAGGA TCAGGGACAG ATCAAGGACA GCACAGAGGA
    TCCGTAGGGC AGCGCAAAGG GTCAGGAGAA CGCAAAATGT CAGCTTCAGA GTATGGACCA
    CATCAAGAGT CCATAACAGA AGAGCCCCTC ACTGCGTCAG AACCAGGACC GCAAATAGAC
    ACTATTCAAG AACTGGATGA AGACTCAACT CCACAATTAG AAGACGATAG TGCCCTAAAA
    GAAAGTAAAA CCTCGGAACT CACTAAAATA GAAACTCAAG AAGAAAAGCC TTTGCTGCTC
    ATCAACGAAG AGCAAGTCCC CAGAGATTCC AAACAGCCAG AAGTTCCCAC ATCCAGCAAA
    AAGGAACGTC CATCAGGCAG CCCCAAAAAA GGGCGCCTTT CAGGAACTTC AAAAAGGGAT
    TCTCAGAAAG ACAAAGCCTG TGAACCAAAG CCGCAACATG TAGAAGGAAA AAAATGGTCA
    GGAGAATTTT TGATCTGTGA CTGGAAGATA AAGCATGTCA AATCTGAAGA GGAGGAACAG
    GCCAAGTTAA TCTGTGATGA CACCAGATTC ACAGACCTAC ATGCGACTAT TAGGAATTTT
    CAGACTTACA AGGGGATCAA AGGGAGGAGT GCCTTCAATG GAGTCTCCCT CGATTTGCTG
    CAGTTTGTAC AGCTCCTGGA GACATTTGTT GGTGAAGACA CCTCCTTCAG TCTCAGCAAG
    GACCTCGCCT CCTTTTTTCA GAAGAACTAT TCTGAAACAA AGCAAGAGAA AATTAAAGCC
    TTAGAACAGG CCAGACAAAA TTCCTCCCGC ATCCGACGGA GAATTCTCCT CCAAGCCATC
    TTTGAGAAGT GGGACAATGA TGGCTCAGGC TTTCTGGATC TGAATGAAGT TGATGATCTC
    CTGTATACAT ATAAGGAGGG AATGGAAAGA GAATCTATGA AGAAAGCTAA ACTGCACATA
    AATTTTCCAG AGCCAAGTCC CGGTCACGAA GTTAAACTGT CTTCAAAGCA ATTTCAGAGG
    TACATAGAAC TGGTCGTATC TGAACTCAGG GGCAATGAAG ACGAAGTTCT GGAAAGTGTT
    GTGGAATTCC TGATGGGTTC TTTAGAGAGG AGCCACGTTG AGGGTCTAAG GAACTGTGCA
    AGGCGGAAAT GGCTCCATCA GATTCAGTAC GCTGCAGAGA CAAGTGGGGT GTCCCTGGAG
    CCGGTGTACA CTGAGACCTT CAGGGTCCTC ACCCAGGACG CTGAGGCTCA CGGAAATAAG
    AAGATCAGTG CTCACATCTC CCTCCTAGAG GAAAACGTGT TCCTGCCTGA GAGAGGGCAC
    GTTCTCTTGA GGAACGTGGC TTGTACTCTA GATGACGCTC CGTTCGTCCT GAACAAAGTG
    CTCTACAGGG ACATGAAGGG AATCAGCTTT ACAGTAGTGG ATGAAGGAAA GCCAATCCAT
    GTCCCCCAAG TTCAGCACCA TGGAAATATC TTCTTCTGGA ACAGTTTCCG AAGTAAGAAT
    GAGTATAACG GGTCGTTCCT GGCTTTGCCT CTTCAGGATG CATACATGAG GATCTTTGGG
    GTCCTGGCAG TTGACACGCT TCGAGATCCT CATGAAATAA ACATCTTCTT ACCTCATGAG
    ATCAAATTCT ATCAGGGGGT CGCCAATGCC TTCAGCACAG CTTACCACCA TGTTCACAGC
    AGGGAACACG TGCTGCACTC CGTGATGACT GGCATCCGGT GGCTCTTCAG TGTCACATCT
    GGCATCACCA CCATCACTAC ATGCTTCATC GAACCCAGCT CAGAACAGGA AGACTATGTT
    TTGCGCAATA TGATGGTTAC AGATTGTCTC GGTCTAGCAG AAATCCACAC AGATCCCCCG
    ACCATCACGA GGAACGCATG CATCTTTAGG GACTTCCTCT TTAAATGCAC AGACACTTCA
    GAAGTTATTT TGGCTTCTTC CGGTGGAGAA ACCCACATTG CCATTCCACT TCGCCAGAGA
    ACCAAAGAGG CTATGGGCAT CCTCGATGTT AACATTGGCC GGAGCAGGAT GCTGCTGTAC
    CAAGAGTATA AAGATCTACA GAAAATGGTG AAGATGATCC AAAACGTCTC CTATGAAATA
    CTGGGCGAAT TCTCTGGCGA AATCGAGAAA ACTATGGTTA TAGAGATGGA ATCGGCAGGA
    GAGGTGAAGC GGGCTGGGAT CCTTTTCTTC CGAACCATGC TGCAGGAGCT GCAGGAATGC
    CTCTGCCTCC TCGATTCCAT GGACTTTGTG TCGCTGCTTC TCTATGAGCA CAAATATCAT
    GTCGACTCCA TCCTTCAGGA CATCACTCTC CAGGAGGTGG AAGCCAATGT GGCCCTGGTG
    CACGACGTCC TGAAAGGAGT TATTCTGTTC TCTCAGCGGG AGAAAGACTC TTTAAGCGAC
    TTGGAAGAGT GGGAAAAATG GAAGTTTCAT ATTAACAAGT ACTTAGTGGA GGAGATTTGT
    GTGCTTGATC CGACTGCTAG CAACGTGGAG GTTAATGTAG AGCTCGTTAC AAGCTATATC
    CAAGCTCATT CTCGGACTGA AGTCTGGAAT TTCAGGAACA TTGTCATTGA ACTCCTGTAT
    CACTGGATAA ACATCTGTCT GACTCTCATA GAGCTAAACA TGCGACAAGA TGTTTCCATT
    ATCCCGCCAT TGCCCAAGAA GAGTGCCACC TCTATTTATG CAATATCCTC AGAAAGATCA
    ATCCGAGAGA AACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77941
    Clone ID Related Accession (Same CDS sequence) XM_017314598.1
    Accession Version XM_017314598.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGCTC ATTCTCGGAC TGAAGTCTGG
    AATTTCAGGA ACATTGTCAT TGAACTCCTG TATCACTGGA TAAACATCTG TCTGACTCTC
    ATAGAGCTAA ACATGCGACA AGATGTTTCC ATTATCCCGC CATTGCCCAA GAAGAGTGCC
    ACCTCTATTT ATGCAATATC CTCAGAAAGA TCAATCCGAG AGAAACTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170087.1
    CDS442..4992
    Translation

    Target ORF information:

    RefSeq Version XM_017314598.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314598.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGGAAGACTA TGTTTTGCGC
    AATATGATGG TTACAGATTG TCTCGGTCTA GCAGAAATCC ACACAGATCC CCCGACCATC
    ACGAGGAACG CATGCATCTT TAGGGACTTC CTCTTTAAAT GCACAGACAC TTCAGAAGTT
    ATTTTGGCTT CTTCCGGTGG AGAAACCCAC ATTGCCATTC CACTTCGCCA GAGAACCAAA
    GAGGCTATGG GCATCCTCGA TGTTAACATT GGCCGGAGCA GGATGCTGCT GTACCAAGAG
    TATAAAGATC TACAGAAAAT GGTGAAGATG ATCCAAAACG TCTCCTATGA AATACTGGGC
    GAATTCTCTG GCGAAATCGA GAAAACTATG GTTATAGCTC ATTCTCGGAC TGAAGTCTGG
    AATTTCAGGA ACATTGTCAT TGAACTCCTG TATCACTGGA TAAACATCTG TCTGACTCTC
    ATAGAGCTAA ACATGCGACA AGATGTTTCC ATTATCCCGC CATTGCCCAA GAAGAGTGCC
    ACCTCTATTT ATGCAATATC CTCAGAAAGA TCAATCCGAG AGAAACTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77942
    Clone ID Related Accession (Same CDS sequence) XM_017314599.1 , XM_017314599.2
    Accession Version XM_017314599.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGCTCATTCT CGGACTGAAG
    TCTGGAATTT CAGGAACATT GTCATTGAAC TCCTGTATCA CTGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170088.1
    CDS445..4512
    Translation

    Target ORF information:

    RefSeq Version XM_017314599.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314599.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTGAAT CAACATCTCA AGATCAGTCC AAGCCTGACC AGGACAATGG AAAAGAAGAC 
    AGGGAACAGC AGCAGGACTC CTCAAAGGAC AGAAAGGACG AAAAGAATGA AAAGGACTTA
    AAGGAAACAA AGGAGGAAAA GGACAAAAAG GAAGAAAAGA ACAAAAAGGA CAAAAAGAAG
    GAAAAGGACA AACAGGACAA AAAGGAGGAA GAGGACAAAA AGGAGGAAGA GGACAAAAAG
    GAGGAAGAGG ACACAAAGGA GGAAGAGGAC AAAGAGGACG AAAAGGAGCC ATCAACAAAT
    CCACCAAGTG ATGTTGATTC AGAAACTAGC AACCAAGTGA CAGAAAAGGC AACGGTGGAT
    ATGAAATTCC AAGCTGGTTC AACAAAGGAC TCCCCTGCAG TTTCCAGGAA TGTTATGCTT
    AAAAAGCCTG CAAAAGTGAT TCTTGGTATA GACGAAAGTG ATCTGAGGTT CAAAGTACAG
    CTGCCGTGGA AGAAAAATCT TAATTTGAGG ATAGAGGAAA GAGCCCAGGC CAAGCAACAG
    AAAATATTAG AGAACGATAA ACTGAAGAAG GAACAGGAAA AGAAGGTTAC AAAAAAGCTC
    CCTAGGGATG ACTTGGCTAA AGAGTGGTTC AATCCTGAAA GCATGACACT GAATACCCGG
    GCGTACCTGC TGGACAAGCT TCTACCCACC TTGGTTCCCG GAGTGGAGCA AATGCTGATG
    CAGGTGGAGA AGAAGAAGCT TTTGAGCCAA GCTGACATCC CAACCAAGTT TGATCCAATT
    AATTATTTGG GGGAATATTT AATGAGACAC AACACTCATT ATATCAAAGA CCCAGGAGTG
    TCTGGCTACC AGAGGGTGAT GAAAGAGATC ACTGAAGAAC TGAAGACTCA TGTCCCTGAT
    ACGATCAACA ACAGAATATC CAAGATGAAG GAGAAAGTGA AAGAGAAGAG AGAACAAAGA
    GAGTACATAA GCACAGTCAA AGTCAAGGTG GCCGGCATGA GAAAGCAGGC TCTGGAAGAG
    CAGTTCAATG AATGGGTTCT CAACCCCAAA GGGATGATTC CAATAGTCGT GTACATTTCT
    TCATACATCA CAGACTTGAA AAGCGAAGTC TTTGAGGAAT TCCTTAAACA CCTGTGCCAC
    AGCGCAGAAG AATTCCGGGA GATCATACTG ACTGACATGC GGAGGCAGAT GTTTGCAGAA
    CTCTTCCTAC AGTGCGACAG TGGGAAGGTG ATGGCCTTGG ATCGGCAGAG GACACTGGCC
    CTGCTGGAAG CGTTCTATGA TCAATGCTCT TCCACCACTA GGAGTTTACT CCGAAACCCA
    CGACAATGGC CCTTTGTTGA ATTTGAAGAG ATAGAGTTGA TTGAGTTCTG GGGAGACATG
    GATATTAAGA AGCACATTTA TGAAGACTTT GATGCACTGC TCTTAAAGAT GAATATGTTA
    GTTGCTGAAA AACTTGCAGG CAAACTCGCA GAAAACAAAG ATCTCCCACA GCAGCAGAGA
    GATCAAGAAC TTTCATCTGA CTCAACAACG GAGCCAGAAA CTGCCACACA ACTGACCTCA
    CAACAGAGAT CCCGCAGAGT ATCACTTACA GGACAAGGAC AGGGCAAAGG GCCTCGCAAA
    ACATCTGCTT CAAAACAAGG AGCAAGCAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCAGCAG TAGAACAAAC CCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC TCGGCAGAGA GGGTCAGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCTACAG TAGAACAAAC CCAGCGGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGCTCGGGAG TGAACCAAAC CCAGCAGAGA GGGTCGGTGG CAGAACAAGG ATCCCGAAGA
    AGCTCGGCAG TAGAACAAAC TCAGCAGAGA GGGTCGGTGG CAGAGCAAGG GTCCCGAAGA
    AGTTCAGCCA TGGAACAAGA GCCACAAACA GCACAAGATC CAAACAGCGA TTCATTGCCA
    GAACAAGAAT CACACAGAGG GTCCATCACA GAAGGATCAC ACAGAGGGTC AATTTCAGAA
    CCTGGGCAAC GCAGAGCATC AGTAACAGGA CAACGCAGAA AGTCGTCTGT AGATGACTCC
    GGTAGTGCAG GGTCACGCAG AGGATCAGGG ACAGATCAAG GACAGCACAG AGGATCCGTA
    GGGCAGCGCA AAGGGTCAGG AGAACGCAAA ATGTCAGCTT CAGAGTATGG ACCACATCAA
    GAGTCCATAA CAGAAGAGCC CCTCACTGCG TCAGAACCAG GACCGCAAAT AGACACTATT
    CAAGAACTGG ATGAAGACTC AACTCCACAA TTAGAAGACG ATAGTGCCCT AAAAGAAAGT
    AAAACCTCGG AACTCACTAA AATAGAAACT CAAGAAGAAA AGCCTTTGCT GCTCATCAAC
    GAAGAGCAAG TCCCCAGAGA TTCCAAACAG CCAGAAGTTC CCACATCCAG CAAAAAGGAA
    CGTCCATCAG GCAGCCCCAA AAAAGGGCGC CTTTCAGGAA CTTCAAAAAG GGATTCTCAG
    AAAGACAAAG CCTGTGAACC AAAGCCGCAA CATGTAGAAG GAAAAAAATG GTCAGGAGAA
    TTTTTGATCT GTGACTGGAA GATAAAGCAT GTCAAATCTG AAGAGGAGGA ACAGGCCAAG
    TTAATCTGTG ATGACACCAG ATTCACAGAC CTACATGCGA CTATTAGGAA TTTTCAGACT
    TACAAGGGGA TCAAAGGGAG GAGTGCCTTC AATGGAGTCT CCCTCGATTT GCTGCAGTTT
    GTACAGCTCC TGGAGACATT TGTTGGTGAA GACACCTCCT TCAGTCTCAG CAAGGACCTC
    GCCTCCTTTT TTCAGAAGAA CTATTCTGAA ACAAAGCAAG AGAAAATTAA AGCCTTAGAA
    CAGGCCAGAC AAAATTCCTC CCGCATCCGA CGGAGAATTC TCCTCCAAGC CATCTTTGAG
    AAGTGGGACA ATGATGGCTC AGGCTTTCTG GATCTGAATG AAGTTGATGA TCTCCTGTAT
    ACATATAAGG AGGGAATGGA AAGAGAATCT ATGAAGAAAG CTAAACTGCA CATAAATTTT
    CCAGAGCCAA GTCCCGGTCA CGAAGTTAAA CTGTCTTCAA AGCAATTTCA GAGGTACATA
    GAACTGGTCG TATCTGAACT CAGGGGCAAT GAAGACGAAG TTCTGGAAAG TGTTGTGGAA
    TTCCTGATGG GTTCTTTAGA GAGGAGCCAC GTTGAGGGTC TAAGGAACTG TGCAAGGCGG
    AAATGGCTCC ATCAGATTCA GTACGCTGCA GAGACAAGTG GGGTGTCCCT GGAGCCGGTG
    TACACTGAGA CCTTCAGGGT CCTCACCCAG GACGCTGAGG CTCACGGAAA TAAGAAGATC
    AGTGCTCACA TCTCCCTCCT AGAGGAAAAC GTGTTCCTGC CTGAGAGAGG GCACGTTCTC
    TTGAGGAACG TGGCTTGTAC TCTAGATGAC GCTCCGTTCG TCCTGAACAA AGTGCTCTAC
    AGGGACATGA AGGGAATCAG CTTTACAGTA GTGGATGAAG GAAAGCCAAT CCATGTCCCC
    CAAGTTCAGC ACCATGGAAA TATCTTCTTC TGGAACAGTT TCCGAAGTAA GAATGAGTAT
    AACGGGTCGT TCCTGGCTTT GCCTCTTCAG GATGCATACA TGAGGATCTT TGGGGTCCTG
    GCAGTTGACA CGCTTCGAGA TCCTCATGAA ATAAACATCT TCTTACCTCA TGAGATCAAA
    TTCTATCAGG GGGTCGCCAA TGCCTTCAGC ACAGCTTACC ACCATGTTCA CAGCAGGGAA
    CACGTGCTGC ACTCCGTGAT GACTGGCATC CGGTGGCTCT TCAGTGTCAC ATCTGGCATC
    ACCACCATCA CTACATGCTT CATCGAACCC AGCTCAGAAC AGCTCATTCT CGGACTGAAG
    TCTGGAATTT CAGGAACATT GTCATTGAAC TCCTGTATCA CTGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77943
    Clone ID Related Accession (Same CDS sequence) XM_017314601.1
    Accession Version XM_017314601.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3879bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product EF-hand calcium-binding domain-containing protein 5 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTCTGTC ACAACAAATA CATTTCTTCA TACATCACAG ACTTGAAAAG CGAAGTCTTT 
    GAGGAATTCC TTAAACACCT GTGCCACAGC GCAGAAGAAT TCCGGGAGAT CATACTGACT
    GACATGCGGA GGCAGATGTT TGCAGAACTC TTCCTACAGT GCGACAGTGG GAAGGTGATG
    GCCTTGGATC GGCAGAGGAC ACTGGCCCTG CTGGAAGCGT TCTATGATCA ATGCTCTTCC
    ACCACTAGGA GTTTACTCCG AAACCCACGA CAATGGCCCT TTGTTGAATT TGAAGAGATA
    GAGTTGATTG AGTTCTGGGG AGACATGGAT ATTAAGAAGC ACATTTATGA AGACTTTGAT
    GCACTGCTCT TAAAGATGAA TATGTTAGTT GCTGAAAAAC TTGCAGGCAA ACTCGCAGAA
    AACAAAGATC TCCCACAGCA GCAGAGAGAT CAAGAACTTT CATCTGACTC AACAACGGAG
    CCAGAAACTG CCACACAACT GACCTCACAA CAGAGATCCC GCAGAGTATC ACTTACAGGA
    CAAGGACAGG GCAAAGGGCC TCGCAAAACA TCTGCTTCAA AACAAGGAGC AAGCAGAGGG
    TCAGTGGCAG AGCAAGGGTC CCGAAGAAGC TCGGGAGTGA ACCAAACCCA GCAGAGAGGG
    TCGGTGGCAG AACAAGGATC CCGAAGAAGC TCAGCAGTAG AACAAACCCA GCAGAGAGGG
    TCGGTGGCAG AGCAAGGGTC CCGAAGAAGC TCTACAGTAG AACAAACTCG GCAGAGAGGG
    TCAGTGGCAG AGCAAGGGTC CCGAAGAAGC TCTACAGTAG AACAAACCCA GCGGAGAGGG
    TCGGTGGCAG AGCAAGGGTC CCGAAGAAGC TCGGGAGTGA ACCAAACCCA GCAGAGAGGG
    TCGGTGGCAG AACAAGGATC CCGAAGAAGC TCGGCAGTAG AACAAACTCA GCAGAGAGGG
    TCGGTGGCAG AGCAAGGGTC CCGAAGAAGT TCAGCCATGG AACAAGAGCC ACAAACAGCA
    CAAGATCCAA ACAGCGATTC ATTGCCAGAA CAAGAATCAC ACAGAGGGTC CATCACAGAA
    GGATCACACA GAGGGTCAAT TTCAGAACCT GGGCAACGCA GAGCATCAGT AACAGGACAA
    CGCAGAAAGT CGTCTGTAGA TGACTCCGGT AGTGCAGGGT CACGCAGAGG ATCAGGGACA
    GATCAAGGAC AGCACAGAGG ATCCGTAGGG CAGCGCAAAG GGTCAGGAGA ACGCAAAATG
    TCAGCTTCAG AGTATGGACC ACATCAAGAG TCCATAACAG AAGAGCCCCT CACTGCGTCA
    GAACCAGGAC CGCAAATAGA CACTATTCAA GAACTGGATG AAGACTCAAC TCCACAATTA
    GAAGACGATA GTGCCCTAAA AGAAAGTAAA ACCTCGGAAC TCACTAAAAT AGAAACTCAA
    GAAGAAAAGC CTTTGCTGCT CATCAACGAA GAGCAAGTCC CCAGAGATTC CAAACAGCCA
    GAAGTTCCCA CATCCAGCAA AAAGGAACGT CCATCAGGCA GCCCCAAAAA AGGGCGCCTT
    TCAGGAACTT CAAAAAGGGA TTCTCAGAAA GACAAAGCCT GTGAACCAAA GCCGCAACAT
    GTAGAAGGAA AAAAATGGTC AGGAGAATTT TTGATCTGTG ACTGGAAGAT AAAGCATGTC
    AAATCTGAAG AGGAGGAACA GGCCAAGTTA ATCTGTGATG ACACCAGATT CACAGACCTA
    CATGCGACTA TTAGGAATTT TCAGACTTAC AAGGGGATCA AAGGGAGGAG TGCCTTCAAT
    GGAGTCTCCC TCGATTTGCT GCAGTTTGTA CAGCTCCTGG AGACATTTGT TGGTGAAGAC
    ACCTCCTTCA GTCTCAGCAA GGACCTCGCC TCCTTTTTTC AGAAGAACTA TTCTGAAACA
    AAGCAAGAGA AAATTAAAGC CTTAGAACAG GCCAGACAAA ATTCCTCCCG CATCCGACGG
    AGAATTCTCC TCCAAGCCAT CTTTGAGAAG TGGGACAATG ATGGCTCAGG CTTTCTGGAT
    CTGAATGAAG TTGATGATCT CCTGTATACA TATAAGGAGG GAATGGAAAG AGAATCTATG
    AAGAAAGCTA AACTGCACAT AAATTTTCCA GAGCCAAGTC CCGGTCACGA AGTTAAACTG
    TCTTCAAAGC AATTTCAGAG GTACATAGAA CTGGTCGTAT CTGAACTCAG GGGCAATGAA
    GACGAAGTTC TGGAAAGTGT TGTGGAATTC CTGATGGGTT CTTTAGAGAG GAGCCACGTT
    GAGGGTCTAA GGAACTGTGC AAGGCGGAAA TGGCTCCATC AGATTCAGTA CGCTGCAGAG
    ACAAGTGGGG TGTCCCTGGA GCCGGTGTAC ACTGAGACCT TCAGGGTCCT CACCCAGGAC
    GCTGAGGCTC ACGGAAATAA GAAGATCAGT GCTCACATCT CCCTCCTAGA GGAAAACGTG
    TTCCTGCCTG AGAGAGGGCA CGTTCTCTTG AGGAACGTGG CTTGTACTCT AGATGACGCT
    CCGTTCGTCC TGAACAAAGT GCTCTACAGG GACATGAAGG GAATCAGCTT TACAGTAGTG
    GATGAAGGAA AGCCAATCCA TGTCCCCCAA GTTCAGCACC ATGGAAATAT CTTCTTCTGG
    AACAGTTTCC GAAGTAAGAA TGAGTATAAC GGGTCGTTCC TGGCTTTGCC TCTTCAGGAT
    GCATACATGA GGATCTTTGG GGTCCTGGCA GTTGACACGC TTCGAGATCC TCATGAAATA
    AACATCTTCT TACCTCATGA GATCAAATTC TATCAGGGGG TCGCCAATGC CTTCAGCACA
    GCTTACCACC ATGTTCACAG CAGGGAACAC GTGCTGCACT CCGTGATGAC TGGCATCCGG
    TGGCTCTTCA GTGTCACATC TGGCATCACC ACCATCACTA CATGCTTCAT CGAACCCAGC
    TCAGAACAGG AAGACTATGT TTTGCGCAAT ATGATGGTTA CAGATTGTCT CGGTCTAGCA
    GAAATCCACA CAGATCCCCC GACCATCACG AGGAACGCAT GCATCTTTAG GGACTTCCTC
    TTTAAATGCA CAGACACTTC AGAAGTTATT TTGGCTTCTT CCGGTGGAGA AACCCACATT
    GCCATTCCAC TTCGCCAGAG AACCAAAGAG GCTATGGGCA TCCTCGATGT TAACATTGGC
    CGGAGCAGGA TGCTGCTGTA CCAAGAGTAT AAAGATCTAC AGAAAATGGT GAAGATGATC
    CAAAACGTCT CCTATGAAAT ACTGGGCGAA TTCTCTGGCG AAATCGAGAA AACTATGGTT
    ATAGAGATGG AATCGGCAGG AGAGGTGAAG CGGGCTGGGA TCCTTTTCTT CCGAACCATG
    CTGCAGGAGC TGCAGGAATG CCTCTGCCTC CTCGATTCCA TGGACTTTGT GTCGCTGCTT
    CTCTATGAGC ACAAATATCA TGTCGACTCC ATCCTTCAGG ACATCACTCT CCAGGAGGTG
    GAAGCCAATG TGGCCCTGGT GCACGACGTC CTGAAAGGAG TTATTCTGTT CTCTCAGCGG
    GAGAAAGACT CTTTAAGCGA CTTGGAAGAG TGGGAAAAAT GGAAGTTTCA TATTAACAAG
    TACTTAGTGG AGGAGATTTG TGTGCTTGAT CCGACTGCTA GCAACGTGGA GGTTAATGTA
    GAGCTCGTTA CAAGCTATAT CCAAGCTCAT TCTCGGACTG AAGTCTGGAA TTTCAGGAAC
    ATTGTCATTG AACTCCTGTA TCACTGGATA AACATCTGTC TGACTCTCAT AGAGCTAAAC
    ATGCGACAAG ATGTTTCCAT TATCCCGCCA TTGCCCAAGA AGAGTGCCAC CTCTATTTAT
    GCAATATCCT CAGAAAGATC AATCCGAGAG AAACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170090.1
    CDS31..3909
    Translation

    Target ORF information:

    RefSeq Version XM_017314601.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus EF-hand calcium binding domain 5 (Efcab5), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314601.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTCTGTC ACAACAAATA CATTTCTTCA TACATCACAG ACTTGAAAAG CGAAGTCTTT 
    GAGGAATTCC TTAAACACCT GTGCCACAGC GCAGAAGAAT TCCGGGAGAT CATACTGACT
    GACATGCGGA GGCAGATGTT TGCAGAACTC TTCCTACAGT GCGACAGTGG GAAGGTGATG
    GCCTTGGATC GGCAGAGGAC ACTGGCCCTG CTGGAAGCGT TCTATGATCA ATGCTCTTCC
    ACCACTAGGA GTTTACTCCG AAACCCACGA CAATGGCCCT TTGTTGAATT TGAAGAGATA
    GAGTTGATTG AGTTCTGGGG AGACATGGAT ATTAAGAAGC ACATTTATGA AGACTTTGAT
    GCACTGCTCT TAAAGATGAA TATGTTAGTT GCTGAAAAAC TTGCAGGCAA ACTCGCAGAA
    AACAAAGATC TCCCACAGCA GCAGAGAGAT CAAGAACTTT CATCTGACTC AACAACGGAG
    CCAGAAACTG CCACACAACT GACCTCACAA CAGAGATCCC GCAGAGTATC ACTTACAGGA
    CAAGGACAGG GCAAAGGGCC TCGCAAAACA TCTGCTTCAA AACAAGGAGC AAGCAGAGGG
    TCAGTGGCAG AGCAAGGGTC CCGAAGAAGC TCGGGAGTGA ACCAAACCCA GCAGAGAGGG
    TCGGTGGCAG AACAAGGATC CCGAAGAAGC TCAGCAGTAG AACAAACCCA GCAGAGAGGG
    TCGGTGGCAG AGCAAGGGTC CCGAAGAAGC TCTACAGTAG AACAAACTCG GCAGAGAGGG
    TCAGTGGCAG AGCAAGGGTC CCGAAGAAGC TCTACAGTAG AACAAACCCA GCGGAGAGGG
    TCGGTGGCAG AGCAAGGGTC CCGAAGAAGC TCGGGAGTGA ACCAAACCCA GCAGAGAGGG
    TCGGTGGCAG AACAAGGATC CCGAAGAAGC TCGGCAGTAG AACAAACTCA GCAGAGAGGG
    TCGGTGGCAG AGCAAGGGTC CCGAAGAAGT TCAGCCATGG AACAAGAGCC ACAAACAGCA
    CAAGATCCAA ACAGCGATTC ATTGCCAGAA CAAGAATCAC ACAGAGGGTC CATCACAGAA
    GGATCACACA GAGGGTCAAT TTCAGAACCT GGGCAACGCA GAGCATCAGT AACAGGACAA
    CGCAGAAAGT CGTCTGTAGA TGACTCCGGT AGTGCAGGGT CACGCAGAGG ATCAGGGACA
    GATCAAGGAC AGCACAGAGG ATCCGTAGGG CAGCGCAAAG GGTCAGGAGA ACGCAAAATG
    TCAGCTTCAG AGTATGGACC ACATCAAGAG TCCATAACAG AAGAGCCCCT CACTGCGTCA
    GAACCAGGAC CGCAAATAGA CACTATTCAA GAACTGGATG AAGACTCAAC TCCACAATTA
    GAAGACGATA GTGCCCTAAA AGAAAGTAAA ACCTCGGAAC TCACTAAAAT AGAAACTCAA
    GAAGAAAAGC CTTTGCTGCT CATCAACGAA GAGCAAGTCC CCAGAGATTC CAAACAGCCA
    GAAGTTCCCA CATCCAGCAA AAAGGAACGT CCATCAGGCA GCCCCAAAAA AGGGCGCCTT
    TCAGGAACTT CAAAAAGGGA TTCTCAGAAA GACAAAGCCT GTGAACCAAA GCCGCAACAT
    GTAGAAGGAA AAAAATGGTC AGGAGAATTT TTGATCTGTG ACTGGAAGAT AAAGCATGTC
    AAATCTGAAG AGGAGGAACA GGCCAAGTTA ATCTGTGATG ACACCAGATT CACAGACCTA
    CATGCGACTA TTAGGAATTT TCAGACTTAC AAGGGGATCA AAGGGAGGAG TGCCTTCAAT
    GGAGTCTCCC TCGATTTGCT GCAGTTTGTA CAGCTCCTGG AGACATTTGT TGGTGAAGAC
    ACCTCCTTCA GTCTCAGCAA GGACCTCGCC TCCTTTTTTC AGAAGAACTA TTCTGAAACA
    AAGCAAGAGA AAATTAAAGC CTTAGAACAG GCCAGACAAA ATTCCTCCCG CATCCGACGG
    AGAATTCTCC TCCAAGCCAT CTTTGAGAAG TGGGACAATG ATGGCTCAGG CTTTCTGGAT
    CTGAATGAAG TTGATGATCT CCTGTATACA TATAAGGAGG GAATGGAAAG AGAATCTATG
    AAGAAAGCTA AACTGCACAT AAATTTTCCA GAGCCAAGTC CCGGTCACGA AGTTAAACTG
    TCTTCAAAGC AATTTCAGAG GTACATAGAA CTGGTCGTAT CTGAACTCAG GGGCAATGAA
    GACGAAGTTC TGGAAAGTGT TGTGGAATTC CTGATGGGTT CTTTAGAGAG GAGCCACGTT
    GAGGGTCTAA GGAACTGTGC AAGGCGGAAA TGGCTCCATC AGATTCAGTA CGCTGCAGAG
    ACAAGTGGGG TGTCCCTGGA GCCGGTGTAC ACTGAGACCT TCAGGGTCCT CACCCAGGAC
    GCTGAGGCTC ACGGAAATAA GAAGATCAGT GCTCACATCT CCCTCCTAGA GGAAAACGTG
    TTCCTGCCTG AGAGAGGGCA CGTTCTCTTG AGGAACGTGG CTTGTACTCT AGATGACGCT
    CCGTTCGTCC TGAACAAAGT GCTCTACAGG GACATGAAGG GAATCAGCTT TACAGTAGTG
    GATGAAGGAA AGCCAATCCA TGTCCCCCAA GTTCAGCACC ATGGAAATAT CTTCTTCTGG
    AACAGTTTCC GAAGTAAGAA TGAGTATAAC GGGTCGTTCC TGGCTTTGCC TCTTCAGGAT
    GCATACATGA GGATCTTTGG GGTCCTGGCA GTTGACACGC TTCGAGATCC TCATGAAATA
    AACATCTTCT TACCTCATGA GATCAAATTC TATCAGGGGG TCGCCAATGC CTTCAGCACA
    GCTTACCACC ATGTTCACAG CAGGGAACAC GTGCTGCACT CCGTGATGAC TGGCATCCGG
    TGGCTCTTCA GTGTCACATC TGGCATCACC ACCATCACTA CATGCTTCAT CGAACCCAGC
    TCAGAACAGG AAGACTATGT TTTGCGCAAT ATGATGGTTA CAGATTGTCT CGGTCTAGCA
    GAAATCCACA CAGATCCCCC GACCATCACG AGGAACGCAT GCATCTTTAG GGACTTCCTC
    TTTAAATGCA CAGACACTTC AGAAGTTATT TTGGCTTCTT CCGGTGGAGA AACCCACATT
    GCCATTCCAC TTCGCCAGAG AACCAAAGAG GCTATGGGCA TCCTCGATGT TAACATTGGC
    CGGAGCAGGA TGCTGCTGTA CCAAGAGTAT AAAGATCTAC AGAAAATGGT GAAGATGATC
    CAAAACGTCT CCTATGAAAT ACTGGGCGAA TTCTCTGGCG AAATCGAGAA AACTATGGTT
    ATAGAGATGG AATCGGCAGG AGAGGTGAAG CGGGCTGGGA TCCTTTTCTT CCGAACCATG
    CTGCAGGAGC TGCAGGAATG CCTCTGCCTC CTCGATTCCA TGGACTTTGT GTCGCTGCTT
    CTCTATGAGC ACAAATATCA TGTCGACTCC ATCCTTCAGG ACATCACTCT CCAGGAGGTG
    GAAGCCAATG TGGCCCTGGT GCACGACGTC CTGAAAGGAG TTATTCTGTT CTCTCAGCGG
    GAGAAAGACT CTTTAAGCGA CTTGGAAGAG TGGGAAAAAT GGAAGTTTCA TATTAACAAG
    TACTTAGTGG AGGAGATTTG TGTGCTTGAT CCGACTGCTA GCAACGTGGA GGTTAATGTA
    GAGCTCGTTA CAAGCTATAT CCAAGCTCAT TCTCGGACTG AAGTCTGGAA TTTCAGGAAC
    ATTGTCATTG AACTCCTGTA TCACTGGATA AACATCTGTC TGACTCTCAT AGAGCTAAAC
    ATGCGACAAG ATGTTTCCAT TATCCCGCCA TTGCCCAAGA AGAGTGCCAC CTCTATTTAT
    GCAATATCCT CAGAAAGATC AATCCGAGAG AAACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.