Mmp20 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mmp20
Entrez Gene ID	30800
Full Name	matrix metallopeptidase 20 (enamelysin)
General protein information	Preferred Names matrix metallopeptidase 20 (enamelysin) Names matrix metalloproteinase-20 enamel metalloproteinase matrix metalloproteinase 20 (enamelysin)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A1
Summary	This gene encodes a member of the matrix metalloproteinase family of zinc-dependent endopeptidases capable of degrading extracellular matrix proteins. This gene is expressed specifically in the ameloblasts and odontoblasts, and the encoded protein is an inactive zymogen that requires proteolytic removal of a N-terminal propeptide to become enzymatically active. Mice lacking the encoded protein display an amelogenesis imperfecta phenotype. [provided by RefSeq, Oct 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013903.2	NP_038931.1	matrix metalloproteinase-20 preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	MMP20	NP_004762.2
Pan troglodytes (chimpanzee)	MMP20	XP_001153208.1
Bos taurus (cattle)	MMP20	NP_776816.1
Danio rerio (zebrafish)	LOC100329766	XP_002664668.3
	MMP20	XP_001097602.1
Canis lupus familiaris (dog)	MMP20	XP_854639.1
Rattus norvegicus (Norway rat)	Mmp20	NP_001100270.1
Mus musculus (house mouse)	Mmp20	NP_038931.1
Xenopus tropicalis (tropical clawed frog)	mmp20	XP_002934967.2

Gene Ontology
Bibliography

Related articles in PubMed

A cytoplasmic role of Wnt/?-catenin transcriptional cofactors Bcl9, Bcl9l, and Pygopus in tooth enamel formation.
Cant? C, Pagella P, Shajiei TD, Zimmerli D, Valenta T, Hausmann G, Basler K, Mitsiadis TA
Science signaling10(465)(2017 Feb)

Murine matrix metalloproteinase-20 overexpression stimulates cell invasion into the enamel layer via enhanced Wnt signaling.
Shin M, Suzuki M, Guan X, Smith CE, Bartlett JD
Scientific reports629492(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MMP-20 action guides the growth morphology of the forming hydroxyapatite crystals and enhances their crystallinity.
Title: Matrix metalloproteinase-20 mediates dental enamel biomineralization by preventing protein occlusion inside apatite crystals.

Matrix metalloproteinase-20 over-expression is detrimental to enamel development
Title: Matrix metalloproteinase-20 over-expression is detrimental to enamel development: a Mus musculus model.

enamel of M180Tg/AmelxKO mice was thinner than WT, it had similar mechanical properties and decussating enamel prisms, which were abolished by the loss of MMP20 in the M180Tg/DKO mice.
Title: M180 amelogenin processed by MMP20 is sufficient for decussating murine enamel.

MMP20 cleaves extracellular domains of cadherin adherens junction proteins, both E- and N-cadherin transcripts are expressed at significantly higher levels in Mmp20 null mice; in Mmp20 ablated mice N-cadherin expression persists during maturation stage
Title: MMP20 modulates cadherin expression in ameloblasts as enamel develops.

required for ameloblast cell movement necessary to form the decussating enamel rod patterns, for the prevention of ectopic mineral formation, and to maintain a functional dentin-enamel junction
Title: Matrix metalloproteinase 20 promotes a smooth enamel surface, a strong dentino-enamel junction, and a decussating enamel rod pattern.

The following Mmp20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mmp20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15953	NM_013903.2 Latest version!	Mus musculus matrix metallopeptidase 20 (enamelysin) (Mmp20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$286.30 $409.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mmp20 ( NM_013903.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038931.1 Mus musculus matrix metallopeptidase 20 (enamelysin) (Mmp20), mRNA.

Price & Availability↑

CloneID	OMu15953
Clone ID Related Accession (Same CDS sequence)	NM_013903.2
Accession Version	NM_013903.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	matrix metalloproteinase-20 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL603630.11. On Nov 17, 2006 this sequence version replaced NM_013903.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF155933.1, BC152335.1 [ECO:0000332] ##Evidence-Data-END##

ATGAAGGTGC TACCTGCCTC TGGCCTTGCT GTCCTTGTCA CAGCTTTAAA GTTTGCCACT 
GCAGACCCCA ACCTACTGGC AGCCACCCCT AGGACCTTCA GGAGCAACTA CCACCTTGCA 
CAGGCCTATC TTGACAAGTA TTATACAAAG AAAGGAGGCC CCCAGGCTGG CGAGATGGTG 
GCAAGAGAAA GCAACCCCAT GATCAGGAGG ATTAAGGAGC TACAAATATT CTTTGGCCTC 
AAAGTCACTG GGAAGTTGGA CCAGAATACA ATGAATGTGA TCAAGAAGCC TCGCTGTGGA 
GTTCCTGATG TGGCTAACTA CCGCCTCTTC CCAGGTGAAC CCAAATGGAA AAAAAATATT 
TTGACATACA GAATCTCTAA GTACACACCC TCCATGAGTC CCACTGAGGT GGACAAGGCA 
ATACAGATGG CCCTGCATGC GTGGAGTACA GCTGTCCCTC TGAACTTCGT TAGAATAAAC 
TCTGGGGAAG CAGATATCAT GATATCTTTT GAAACTGGAG ATCATGGGGA TTCCTACCCA 
TTTGATGGGC CTCGAGGGAC TCTGGCCCAT GCATTTGCTC CTGGAGAAGG CCTGGGAGGA 
GACACACATT TCGACAATGC TGAGAAGTGG ACTATGGGAA CGAATGGGTT CAATTTATTC 
ACCGTTGCTG CTCATGAATT TGGCCATGCT CTGGGCCTGG GCCATTCCAC AGATCCATCA 
GCACTGATGT ACCCAACTTA TAAGTACCAG AACCCCTATA GATTTCACCT CCCCAAGGAT 
GATGTGAAAG GGATCCAAGC ATTATACGGA CCCCGGAAAA TATTCCCAGG AAAGCCCACC 
ATGCCCCATA TCCCACCTCA CAAGCCATCT ATCCCCGACC TCTGTGACTC CAGCTCGTCC 
TTTGATGCAG TGACAATGTT GGGAAAGGAG CTTCTGTTCT TCAAGGATAG GATTTTCTGG 
AGAAGGCAGG TGCACTTGCC AACAGGGATT CGGCCCAGCA CCATCACCAG CTCCTTTCCC 
CAGCTAATGT CCAATGTGGA CGCGGCTTAC GAAGTGGCTG AACGAGGCAT TGCTTTCTTC 
TTCAAAGGTC CTCACTACTG GGTCACTCGA GGATTCCATA TGCAAGGTCC TCCACGGACA 
ATTTATGACT TCGGGTTCCC AAGGCATGTG CAACGAATAG ATGCTGCTGT CTACCTCAAG 
GAGCCTCAGA AGACCCTTTT CTTTGTGGGA GAGGAATACT ACAGCTATGA TGAAAGAAAA 
AAGAAAATGG AAAAAGACTA CCCAAAAAAC ACTGAAGAAG AGTTTTCAGG AGTGAGTGGC 
CATATAGATG CTGCTGTGGA ACTGAATGGC TACATTTACT TCTTTTCAGG ACGGAAAACA 
TTCAAGTATG ATACAGAGAA GGAAGACGTG GTTAGTGTGG TAAAATCCAG TTCCTGGATT 
GGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038931.1
CDS	23..1471
Translation	MKVLPASGLAVLVTALKFATADPNLLAATPRTFRSNYHLAQAYLDKYYTKKGGPQAGEMVARESNPMIRRIKELQIFFGLKVTGKLDQNTMNVIKKPRCGVPDVANYRLFPGEPKWKKNILTYRISKYTPSMSPTEVDKAIQMALHAWSTAVPLNFVRINSGEADIMISFETGDHGDSYPFDGPRGTLAHAFAPGEGLGGDTHFDNAEKWTMGTNGFNLFTVAAHEFGHALGLGHSTDPSALMYPTYKYQNPYRFHLPKDDVKGIQALYGPRKIFPGKPTMPHIPPHKPSIPDLCDSSSSFDAVTMLGKELLFFKDRIFWRRQVHLPTGIRPSTITSSFPQLMSNVDAAYEVAERGIAFFFKGPHYWVTRGFHMQGPPRTIYDFGFPRHVQRIDAAVYLKEPQKTLFFVGEEYYSYDERKKKMEKDYPKNTEEEFSGVSGHIDAAVELNGYIYFFSGRKTFKYDTEKEDVVSVVKSSSWIGC

Target ORF information:

RefSeq Version	NM_013903.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus matrix metallopeptidase 20 (enamelysin) (Mmp20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013903.2

ORF Insert Sequence:

ATGAAGGTGC TACCTGCCTC TGGCCTTGCT GTCCTTGTCA CAGCTTTAAA GTTTGCCACT 
GCAGACCCCA ACCTACTGGC AGCCACCCCT AGGACCTTCA GGAGCAACTA CCACCTTGCA 
CAGGCCTATC TTGACAAGTA TTATACAAAG AAAGGAGGCC CCCAGGCTGG CGAGATGGTG 
GCAAGAGAAA GCAACCCCAT GATCAGGAGG ATTAAGGAGC TACAAATATT CTTTGGCCTC 
AAAGTCACTG GGAAGTTGGA CCAGAATACA ATGAATGTGA TCAAGAAGCC TCGCTGTGGA 
GTTCCTGATG TGGCTAACTA CCGCCTCTTC CCAGGTGAAC CCAAATGGAA AAAAAATATT 
TTGACATACA GAATCTCTAA GTACACACCC TCCATGAGTC CCACTGAGGT GGACAAGGCA 
ATACAGATGG CCCTGCATGC GTGGAGTACA GCTGTCCCTC TGAACTTCGT TAGAATAAAC 
TCTGGGGAAG CAGATATCAT GATATCTTTT GAAACTGGAG ATCATGGGGA TTCCTACCCA 
TTTGATGGGC CTCGAGGGAC TCTGGCCCAT GCATTTGCTC CTGGAGAAGG CCTGGGAGGA 
GACACACATT TCGACAATGC TGAGAAGTGG ACTATGGGAA CGAATGGGTT CAATTTATTC 
ACCGTTGCTG CTCATGAATT TGGCCATGCT CTGGGCCTGG GCCATTCCAC AGATCCATCA 
GCACTGATGT ACCCAACTTA TAAGTACCAG AACCCCTATA GATTTCACCT CCCCAAGGAT 
GATGTGAAAG GGATCCAAGC ATTATACGGA CCCCGGAAAA TATTCCCAGG AAAGCCCACC 
ATGCCCCATA TCCCACCTCA CAAGCCATCT ATCCCCGACC TCTGTGACTC CAGCTCGTCC 
TTTGATGCAG TGACAATGTT GGGAAAGGAG CTTCTGTTCT TCAAGGATAG GATTTTCTGG 
AGAAGGCAGG TGCACTTGCC AACAGGGATT CGGCCCAGCA CCATCACCAG CTCCTTTCCC 
CAGCTAATGT CCAATGTGGA CGCGGCTTAC GAAGTGGCTG AACGAGGCAT TGCTTTCTTC 
TTCAAAGGTC CTCACTACTG GGTCACTCGA GGATTCCATA TGCAAGGTCC TCCACGGACA 
ATTTATGACT TCGGGTTCCC AAGGCATGTG CAACGAATAG ATGCTGCTGT CTACCTCAAG 
GAGCCTCAGA AGACCCTTTT CTTTGTGGGA GAGGAATACT ACAGCTATGA TGAAAGAAAA 
AAGAAAATGG AAAAAGACTA CCCAAAAAAC ACTGAAGAAG AGTTTTCAGG AGTGAGTGGC 
CATATAGATG CTGCTGTGGA ACTGAATGGC TACATTTACT TCTTTTCAGG ACGGAAAACA 
TTCAAGTATG ATACAGAGAA GGAAGACGTG GTTAGTGTGG TAAAATCCAG TTCCTGGATT 
GGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A cytoplasmic role of Wnt/?-catenin transcriptional cofactors Bcl9, Bcl9l, and Pygopus in tooth enamel formation.
Science signaling10(465)(2017 Feb)
Cant? C,Pagella P,Shajiei TD,Zimmerli D,Valenta T,Hausmann G,Basler K,Mitsiadis TA

Murine matrix metalloproteinase-20 overexpression stimulates cell invasion into the enamel layer via enhanced Wnt signaling.
Scientific reports629492(2016 Jul)
Shin M,Suzuki M,Guan X,Smith CE,Bartlett JD

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Mmp20 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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