Polk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polk
Entrez Gene ID	27015
Full Name	polymerase (DNA directed), kappa
Synonyms	DINP,Dinb1
General protein information	Preferred Names polymerase (DNA directed), kappa Names DNA polymerase kappa DINB protein DNA damage-inducible protein b DNA-directed DNA polymerase kappa DinB homolog 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001347603.1	NP_001334532.1	DNA polymerase kappa isoform 3
XM_006517680.4	XP_006517743.1	DNA polymerase kappa isoform X2
NM_001347602.1	NP_001334531.1	DNA polymerase kappa isoform 2
NM_012048.3	NP_036178.1	DNA polymerase kappa isoform a
NM_001347604.1	NP_001334533.1	DNA polymerase kappa isoform 8
XM_006517680.3	XP_006517743.1	DNA polymerase kappa isoform X2
NM_001347606.1	NP_001334535.1	DNA polymerase kappa isoform 9
XM_030247285.1	XP_030103145.1	DNA polymerase kappa isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Polk	NP_612525.1
Xenopus tropicalis (tropical clawed frog)	polk	NP_001005705.1
Mus musculus (house mouse)	Polk	NP_036178.1
Caenorhabditis elegans (roundworm)	polk-1	NP_001122692.1
Schizosaccharomyces pombe (fission yeast)	kpa1	NP_587767.2
Homo sapiens (human)	POLK	NP_057302.1
Canis lupus familiaris (dog)	POLK	XP_536321.2
Gallus gallus (chicken)	POLK	NP_989514.1
	POLK	XP_001105220.1
Bos taurus (cattle)	POLK	NP_001095728.1
Magnaporthe oryzae (rice blast fungus)	MGG_07238	XP_003715457.1
Pan troglodytes (chimpanzee)	POLK	XP_001149179.1
Danio rerio (zebrafish)	polk	XP_691219.4
Neurospora crassa	NCU02457	XP_959512.1

Gene Ontology
Bibliography

Related articles in PubMed

Limited ability of DNA polymerase kappa to suppress benzo[a]pyrene-induced genotoxicity in vivo.
Masumura K, Toyoda-Hokaiwado N, Niimi N, Gr?z P, Wada NA, Takeiri A, Jishage KI, Mishima M, Nohmi T
Environmental and molecular mutagenesis58(9)644-653(2017 12)

Effects of the N terminus of mouse DNA polymerase ? on the bypass of a guanine-benzo[a]pyrenyl adduct.
Liu Y, Ma X, Guo C
Journal of biochemistry159(4)471-9(2016 Apr)

Interferon regulatory factor 8 (IRF8) interacts with the B cell lymphoma 6 (BCL6) corepressor BCOR.
Yoon J, Feng X, Kim YS, Shin DM, Hatzi K, Wang H, Morse HC 3rd
The Journal of biological chemistry289(49)34250-7(2014 Dec)

In vivo evidence that DNA polymerase kappa is responsible for error-free bypass across DNA cross-links induced by mitomycin C.
Takeiri A, Wada NA, Motoyama S, Matsuzaki K, Tateishi H, Matsumoto K, Niimi N, Sassa A, Gr?z P, Masumura K, Yamada M, Mishima M, Jishage KI, Nohmi T
DNA repair24113-121(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that Polk has a limited ability to suppress BP-induced genotoxicity in the colon and bone marrow and also that the roles of specialized DNA polymerases in mutagenesis and carcinogenesis should be examined not only by in vitro assays but also by in vivo mouse studies. We also report the spontaneous mutagenesis in inactivated Polk KI mice at young and old ages.
Title: Limited ability of DNA polymerase kappa to suppress benzo[a]pyrene-induced genotoxicity in vivo.

the extreme N-terminal part of Polkappa is required for the processivity and fidelity of Polkappa during translesion synthesis of 10S(+)-trans-anti-benzo[a]pyrene diol epoxide-N(2)-deoxyguanine adducts lesions.
Title: Effects of the N terminus of mouse DNA polymerase ? on the bypass of a guanine-benzo[a]pyrenyl adduct.

Polk plays a predominant role in suppressing point mutations by carrying out error-free translesion DNA synthesis and contributes to the prevention of DNA strand breaks.
Title: In vivo evidence that DNA polymerase kappa is responsible for error-free bypass across DNA cross-links induced by mitomycin C.

The structural gap physically accommodates the bulky aromatic adduct and the N-clasp is essential for the structural integrity and flexibility of Polkappa during translesion synthesis.
Title: Variants of mouse DNA polymerase ? reveal a mechanism of efficient and accurate translesion synthesis past a benzo[a]pyrene dG adduct.

Polkappa accumulates at laser-induced sites of DNA damage.
Title: Mouse DNA polymerase kappa has a functional role in the repair of DNA strand breaks.

The following Polk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu83386	NM_001347606.1 Latest version!	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu22614	NM_012048.3 Latest version!	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu49059	NM_001347602.1 Latest version!	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu83385	NM_001347604.1 Latest version!	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu83387	NM_001347603.1 Latest version!	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu49059	XM_030247285.1 Latest version!	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu49060	XM_006517680.4 Latest version!	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu49060	XM_006517680.3	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polk ( NM_001347606.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334535.1 Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OMu83386
Clone ID Related Accession (Same CDS sequence)	NM_001347606.1
Accession Version	NM_001347606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	DNA polymerase kappa isoform 9
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK138299.1, CN698108.1, DV656157.1, BG061549.2, AK014087.1, BG060790.2 and EH097080.1. Transcript Variant: This variant (9) contains lacks an alternate in-frame exon in both the central and 3' coding regions, compared to variant a, resulting in an isoform (9) that is shorter than isoform a. The 5' UTR of this variant is incomplete because there are no 5'-complete transcripts representing it and there is splicing ambiguity at the 5' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY769927.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164133 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATAACA CAAAGGAAAA GGACAACTTC AAAGACGACC TCCTGCTCCG CATGGGACTA 
AACGATAACA AAGCAGGCAT GGAAGGGTTG GATAAGGAGA AAATTAACAA AATTATCATG 
GAAGCCACAA AGGGGTCCAG ATTTTATGGA AATGAGCTCA AGAAGGAAAA GCAAGTCAAT 
CAACGGATTG AAAATATGAT GCAACAAAAA GCTCAAATTA CCAGCCAGCA ACTAAGGAAA 
GCTCAATTAC AGGTTGACAA ATTTGCAATG GAGTTAGAAC GGAACCGGAA TTTGAACAAT 
ACCATAGTTC ATGTTGACAT GGACGCTTTC TATGCAGCTG TGGAAATGAG GGACAACCCG 
GAACTGAAGG ATAAACCCAT TGCTGTAGGA TCCATGAGCA TGTTGGCTAC TTCGAATTAC 
CATGCAAGGA GGTTTGGTGT CCGTGCAGCC ATGCCAGGAT TTATTGCTAA GAGGCTCTGC 
CCACAACTTA TTATAGTGCC CCCAAACTTT GACAAATATA GAGCTGTGAG TAAGGAGGTT 
AAGGAGATAC TTGCTGAATA TGATCCCAAT TTTATGGCCA TGAGTCTGGA CGAAGCCTAC 
TTGAATATAA CACAGCACTT GCAGGAAAGG CAAGATTGGC CTGAGGACAA AAGAAGATAC 
TTCATCAAGA TGGGAAACTA CTTAAAAATC GGCATCGCCC CCAATACAAT GTTAGCAAAA 
GTGTGCAGTG ATAAGAATAA GCCAAACGGA CAGTACCAGA TCCTTCCCAG CAGGAGCGCG 
GTGATGGACT TCATCAAGGA CCTGCCTATT AGAAAGGTTT CTGGGATAGG AAAAGTTACA 
GAGAAAATGT TAATGGCTCT CGGGATTGTT ACTTGCACAG AACTCTACCA ACAGAGAGCG 
TTGCTGTCTC TCCTTTTCTC TGAAACCTCT TGGCATTATT TTCTTCACAT CGCGCTGGGT 
CTAGGTTCAA CAGACCTGGC AAGGGATGGA GAAAGGAAAA GCATGAGTGT TGAAAGGACA 
TTCAGTGAGA TAAGTAAGAC AGAGGAACAG TACAGCCTGT GCCAAGAACT GTGCGCTGAG 
CTCGCCCACG ACCTCCAGAA GGAAGGACTT AAGGGAAGAA CCGTCACCAT TAAGCTGAAG 
AACGTGAATT TTGAAGTAAA AACTCGTGCA TCTACCGTTC CGGCCGCCAT TTCTACTGCA 
GAGGAAATAT TTGCCATTGC CAAGGAGCTG CTAAGGACAG AAGTTAATGT GGGTTCTCCA 
CACCCCCTGC GGTTAAGACT GATGGACAGA CTTCAGAAGG CTTCAGGAAG GACCAAAAGG 
CCAGGAACGA AGACAAAGAG CTCAACTTTG AAGAAAACAA AGCCCCGAGA TCCCAGACAC 
ACCCTTGATG GATTTTTTAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334535.1
CDS	28..1431
Translation	MDNTKEKDNFKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDKFAMELERNRNLNNTIVHVDMDAFYAAVEMRDNPELKDKPIAVGSMSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILAEYDPNFMAMSLDEAYLNITQHLQERQDWPEDKRRYFIKMGNYLKIGIAPNTMLAKVCSDKNKPNGQYQILPSRSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMSVERTFSEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLRTEVNVGSPHPLRLRLMDRLQKASGRTKRPGTKTKSSTLKKTKPRDPRHTLDGFFK

Target ORF information:

RefSeq Version	NM_001347606.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347606.1

ORF Insert Sequence:

ATGGATAACA CAAAGGAAAA GGACAACTTC AAAGACGACC TCCTGCTCCG CATGGGACTA 
AACGATAACA AAGCAGGCAT GGAAGGGTTG GATAAGGAGA AAATTAACAA AATTATCATG 
GAAGCCACAA AGGGGTCCAG ATTTTATGGA AATGAGCTCA AGAAGGAAAA GCAAGTCAAT 
CAACGGATTG AAAATATGAT GCAACAAAAA GCTCAAATTA CCAGCCAGCA ACTAAGGAAA 
GCTCAATTAC AGGTTGACAA ATTTGCAATG GAGTTAGAAC GGAACCGGAA TTTGAACAAT 
ACCATAGTTC ATGTTGACAT GGACGCTTTC TATGCAGCTG TGGAAATGAG GGACAACCCG 
GAACTGAAGG ATAAACCCAT TGCTGTAGGA TCCATGAGCA TGTTGGCTAC TTCGAATTAC 
CATGCAAGGA GGTTTGGTGT CCGTGCAGCC ATGCCAGGAT TTATTGCTAA GAGGCTCTGC 
CCACAACTTA TTATAGTGCC CCCAAACTTT GACAAATATA GAGCTGTGAG TAAGGAGGTT 
AAGGAGATAC TTGCTGAATA TGATCCCAAT TTTATGGCCA TGAGTCTGGA CGAAGCCTAC 
TTGAATATAA CACAGCACTT GCAGGAAAGG CAAGATTGGC CTGAGGACAA AAGAAGATAC 
TTCATCAAGA TGGGAAACTA CTTAAAAATC GGCATCGCCC CCAATACAAT GTTAGCAAAA 
GTGTGCAGTG ATAAGAATAA GCCAAACGGA CAGTACCAGA TCCTTCCCAG CAGGAGCGCG 
GTGATGGACT TCATCAAGGA CCTGCCTATT AGAAAGGTTT CTGGGATAGG AAAAGTTACA 
GAGAAAATGT TAATGGCTCT CGGGATTGTT ACTTGCACAG AACTCTACCA ACAGAGAGCG 
TTGCTGTCTC TCCTTTTCTC TGAAACCTCT TGGCATTATT TTCTTCACAT CGCGCTGGGT 
CTAGGTTCAA CAGACCTGGC AAGGGATGGA GAAAGGAAAA GCATGAGTGT TGAAAGGACA 
TTCAGTGAGA TAAGTAAGAC AGAGGAACAG TACAGCCTGT GCCAAGAACT GTGCGCTGAG 
CTCGCCCACG ACCTCCAGAA GGAAGGACTT AAGGGAAGAA CCGTCACCAT TAAGCTGAAG 
AACGTGAATT TTGAAGTAAA AACTCGTGCA TCTACCGTTC CGGCCGCCAT TTCTACTGCA 
GAGGAAATAT TTGCCATTGC CAAGGAGCTG CTAAGGACAG AAGTTAATGT GGGTTCTCCA 
CACCCCCTGC GGTTAAGACT GATGGACAGA CTTCAGAAGG CTTCAGGAAG GACCAAAAGG 
CCAGGAACGA AGACAAAGAG CTCAACTTTG AAGAAAACAA AGCCCCGAGA TCCCAGACAC 
ACCCTTGATG GATTTTTTAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polk ( NM_012048.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036178.1 Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant a, mRNA.

Price & Availability↑

CloneID	OMu22614
Clone ID Related Accession (Same CDS sequence)	NM_012048.3
Accession Version	NM_012048.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	DNA polymerase kappa isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK042272.1, AK138299.1, CN698108.1, DV656157.1, BG061549.2, BY707241.1, AK014087.1, BG060790.2 and EH097080.1. On Dec 2, 2016 this sequence version replaced NM_012048.2. Transcript Variant: This variant (a) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF163571.1, AB027563.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164133 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATAACA CAAAGGAAAA GGACAACTTC AAAGACGACC TCCTGCTCCG CATGGGACTA 
AACGATAACA AAGCAGGCAT GGAAGGGTTG GATAAGGAGA AAATTAACAA AATTATCATG 
GAAGCCACAA AGGGGTCCAG ATTTTATGGA AATGAGCTCA AGAAGGAAAA GCAAGTCAAT 
CAACGGATTG AAAATATGAT GCAACAAAAA GCTCAAATTA CCAGCCAGCA ACTAAGGAAA 
GCTCAATTAC AGGTTGACAA ATTTGCAATG GAGTTAGAAC GGAACCGGAA TTTGAACAAT 
ACCATAGTTC ATGTTGACAT GGACGCTTTC TATGCAGCTG TGGAAATGAG GGACAACCCG 
GAACTGAAGG ATAAACCCAT TGCTGTAGGA TCCATGAGCA TGTTGGCTAC TTCGAATTAC 
CATGCAAGGA GGTTTGGTGT CCGTGCAGCC ATGCCAGGAT TTATTGCTAA GAGGCTCTGC 
CCACAACTTA TTATAGTGCC CCCAAACTTT GACAAATATA GAGCTGTGAG TAAGGAGGTT 
AAGGAGATAC TTGCTGAATA TGATCCCAAT TTTATGGCCA TGAGTCTGGA CGAAGCCTAC 
TTGAATATAA CACAGCACTT GCAGGAAAGG CAAGATTGGC CTGAGGACAA AAGAAGATAC 
TTCATCAAGA TGGGAAACTA CTTAAAAATC GACACACCCA GACAGGAAGC TAACGAGCTG 
ACTGAGTATG AGCGGTCCAT CTCCCCGCTG CTTTTTGAAG ATAGTCCTCC TGATTTGCAA 
CCCCAAGGAA GTCCTTTCCA ACTGAACTCT GAAGAACAAA ACAATCCTCA AATAGCCCAA 
AATTCAGTTG TTTTTGGAAC ATCAGCTGAG GAAGTGGTAA AGGAAATTCG CTTCAGAATT 
GAACAAAAAA CAACGCTGAC AGCCAGCGCA GGCATCGCCC CCAATACAAT GTTAGCAAAA 
GTGTGCAGTG ATAAGAATAA GCCAAACGGA CAGTACCAGA TCCTTCCCAG CAGGAGCGCG 
GTGATGGACT TCATCAAGGA CCTGCCTATT AGAAAGGTTT CTGGGATAGG AAAAGTTACA 
GAGAAAATGT TAATGGCTCT CGGGATTGTT ACTTGCACAG AACTCTACCA ACAGAGAGCG 
TTGCTGTCTC TCCTTTTCTC TGAAACCTCT TGGCATTATT TTCTTCACAT CGCGCTGGGT 
CTAGGTTCAA CAGACCTGGC AAGGGATGGA GAAAGGAAAA GCATGAGTGT TGAAAGGACA 
TTCAGTGAGA TAAGTAAGAC AGAGGAACAG TACAGCCTGT GCCAAGAACT GTGCGCTGAG 
CTCGCCCACG ACCTCCAGAA GGAAGGACTT AAGGGAAGAA CCGTCACCAT TAAGCTGAAG 
AACGTGAATT TTGAAGTAAA AACTCGTGCA TCTACCGTTC CGGCCGCCAT TTCTACTGCA 
GAGGAAATAT TTGCCATTGC CAAGGAGCTG CTAAGGACAG AAGTTAATGT GGGTTCTCCA 
CACCCCCTGC GGTTAAGACT GATGGGTGTC CGAATGTCTA CTTTTTCCAG TGAAGATGAC 
AGGAAACACC AACAAAGGAG CATCATTGGT TTCTTACAAG CTGGAAACCA AGCTTTGTCA 
TCTACTGGGG ATAGTCTAGA CAAAACTGAC AAAACTGAGC TTGCAAAGCC CTTAGAAATG 
TCTCATAAGA AGAGTTTCTT TGATAAAAAG CGATCAGAAA GAATCTCCAA CTGTCAAGAC 
ACATCCAGAT GTAAAACTGC GGGTCAGCAA GCTTTACAGA TCTTGGAACC ATCCCAAGCA 
TTAAAGAAGC TGAGCGAGAG TTTTGAAACA TCAGAGAATT CAAATGACTG TCAGACATTT 
ATATGTCCAG TTTGCTTTAG GGAGCAAGAA GGTGTCAGTC TGGAAGCCTT TAATGAACAT 
GTAGATGAGT GTCTTGATGG ACCGTCAACC AGTGAGAACT CAAAAATATC CTGTTACTCA 
CATGCTTCCT CTGCAGACAT TGGTCAGAAG GAAGATGTAC ACCCCTCTAT TCCACTGTGT 
GAGAAACGGG GGCATGAAAA TGGAGAGATC ACTTTAGTAG ATGGTGTAGA TCTAACAGGG 
ACGGAAGACA GATCATTGAA AGCAGCAAGG ATGGACACTC TAGAGAATAA TCGCAGCAAA 
GAGGAATGTC CTGATATTCC AGACAAGTCT TGTCCTATAT CACTGGAAAA TGAAACCATC 
AGTACATTAA GTAGGCAAGA CTCTGTCCAG CCTTGTACAG ATGAGGTAGT AACAGGACGA 
GCTCTAGTGT GTCCTGTTTG TAACCTAGAA CAAGAGACTT CTGATCTTAC CCTCTTCAAC 
ATACATGTGG ATATTTGCTT AAATAAAGGT ATTATCCAAG AACTGAGAAA TAGTGAAGGT 
AATTCAGTTA AACAACCCAA AGAAAGCTCG AGAAGTACTG ACAGACTTCA GAAGGCTTCA 
GGAAGGACCA AAAGGCCAGG AACGAAGACA AAGAGCTCAA CTTTGAAGAA AACAAAGCCC 
CGAGATCCCA GACACACCCT TGATGGATTT TTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036178.1
CDS	281..2839
Translation	MDNTKEKDNFKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDKFAMELERNRNLNNTIVHVDMDAFYAAVEMRDNPELKDKPIAVGSMSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILAEYDPNFMAMSLDEAYLNITQHLQERQDWPEDKRRYFIKMGNYLKIDTPRQEANELTEYERSISPLLFEDSPPDLQPQGSPFQLNSEEQNNPQIAQNSVVFGTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSRSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMSVERTFSEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLRTEVNVGSPHPLRLRLMGVRMSTFSSEDDRKHQQRSIIGFLQAGNQALSSTGDSLDKTDKTELAKPLEMSHKKSFFDKKRSERISNCQDTSRCKTAGQQALQILEPSQALKKLSESFETSENSNDCQTFICPVCFREQEGVSLEAFNEHVDECLDGPSTSENSKISCYSHASSADIGQKEDVHPSIPLCEKRGHENGEITLVDGVDLTGTEDRSLKAARMDTLENNRSKEECPDIPDKSCPISLENETISTLSRQDSVQPCTDEVVTGRALVCPVCNLEQETSDLTLFNIHVDICLNKGIIQELRNSEGNSVKQPKESSRSTDRLQKASGRTKRPGTKTKSSTLKKTKPRDPRHTLDGFFK

Target ORF information:

RefSeq Version	NM_012048.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012048.3

ORF Insert Sequence:

ATGGATAACA CAAAGGAAAA GGACAACTTC AAAGACGACC TCCTGCTCCG CATGGGACTA 
AACGATAACA AAGCAGGCAT GGAAGGGTTG GATAAGGAGA AAATTAACAA AATTATCATG 
GAAGCCACAA AGGGGTCCAG ATTTTATGGA AATGAGCTCA AGAAGGAAAA GCAAGTCAAT 
CAACGGATTG AAAATATGAT GCAACAAAAA GCTCAAATTA CCAGCCAGCA ACTAAGGAAA 
GCTCAATTAC AGGTTGACAA ATTTGCAATG GAGTTAGAAC GGAACCGGAA TTTGAACAAT 
ACCATAGTTC ATGTTGACAT GGACGCTTTC TATGCAGCTG TGGAAATGAG GGACAACCCG 
GAACTGAAGG ATAAACCCAT TGCTGTAGGA TCCATGAGCA TGTTGGCTAC TTCGAATTAC 
CATGCAAGGA GGTTTGGTGT CCGTGCAGCC ATGCCAGGAT TTATTGCTAA GAGGCTCTGC 
CCACAACTTA TTATAGTGCC CCCAAACTTT GACAAATATA GAGCTGTGAG TAAGGAGGTT 
AAGGAGATAC TTGCTGAATA TGATCCCAAT TTTATGGCCA TGAGTCTGGA CGAAGCCTAC 
TTGAATATAA CACAGCACTT GCAGGAAAGG CAAGATTGGC CTGAGGACAA AAGAAGATAC 
TTCATCAAGA TGGGAAACTA CTTAAAAATC GACACACCCA GACAGGAAGC TAACGAGCTG 
ACTGAGTATG AGCGGTCCAT CTCCCCGCTG CTTTTTGAAG ATAGTCCTCC TGATTTGCAA 
CCCCAAGGAA GTCCTTTCCA ACTGAACTCT GAAGAACAAA ACAATCCTCA AATAGCCCAA 
AATTCAGTTG TTTTTGGAAC ATCAGCTGAG GAAGTGGTAA AGGAAATTCG CTTCAGAATT 
GAACAAAAAA CAACGCTGAC AGCCAGCGCA GGCATCGCCC CCAATACAAT GTTAGCAAAA 
GTGTGCAGTG ATAAGAATAA GCCAAACGGA CAGTACCAGA TCCTTCCCAG CAGGAGCGCG 
GTGATGGACT TCATCAAGGA CCTGCCTATT AGAAAGGTTT CTGGGATAGG AAAAGTTACA 
GAGAAAATGT TAATGGCTCT CGGGATTGTT ACTTGCACAG AACTCTACCA ACAGAGAGCG 
TTGCTGTCTC TCCTTTTCTC TGAAACCTCT TGGCATTATT TTCTTCACAT CGCGCTGGGT 
CTAGGTTCAA CAGACCTGGC AAGGGATGGA GAAAGGAAAA GCATGAGTGT TGAAAGGACA 
TTCAGTGAGA TAAGTAAGAC AGAGGAACAG TACAGCCTGT GCCAAGAACT GTGCGCTGAG 
CTCGCCCACG ACCTCCAGAA GGAAGGACTT AAGGGAAGAA CCGTCACCAT TAAGCTGAAG 
AACGTGAATT TTGAAGTAAA AACTCGTGCA TCTACCGTTC CGGCCGCCAT TTCTACTGCA 
GAGGAAATAT TTGCCATTGC CAAGGAGCTG CTAAGGACAG AAGTTAATGT GGGTTCTCCA 
CACCCCCTGC GGTTAAGACT GATGGGTGTC CGAATGTCTA CTTTTTCCAG TGAAGATGAC 
AGGAAACACC AACAAAGGAG CATCATTGGT TTCTTACAAG CTGGAAACCA AGCTTTGTCA 
TCTACTGGGG ATAGTCTAGA CAAAACTGAC AAAACTGAGC TTGCAAAGCC CTTAGAAATG 
TCTCATAAGA AGAGTTTCTT TGATAAAAAG CGATCAGAAA GAATCTCCAA CTGTCAAGAC 
ACATCCAGAT GTAAAACTGC GGGTCAGCAA GCTTTACAGA TCTTGGAACC ATCCCAAGCA 
TTAAAGAAGC TGAGCGAGAG TTTTGAAACA TCAGAGAATT CAAATGACTG TCAGACATTT 
ATATGTCCAG TTTGCTTTAG GGAGCAAGAA GGTGTCAGTC TGGAAGCCTT TAATGAACAT 
GTAGATGAGT GTCTTGATGG ACCGTCAACC AGTGAGAACT CAAAAATATC CTGTTACTCA 
CATGCTTCCT CTGCAGACAT TGGTCAGAAG GAAGATGTAC ACCCCTCTAT TCCACTGTGT 
GAGAAACGGG GGCATGAAAA TGGAGAGATC ACTTTAGTAG ATGGTGTAGA TCTAACAGGG 
ACGGAAGACA GATCATTGAA AGCAGCAAGG ATGGACACTC TAGAGAATAA TCGCAGCAAA 
GAGGAATGTC CTGATATTCC AGACAAGTCT TGTCCTATAT CACTGGAAAA TGAAACCATC 
AGTACATTAA GTAGGCAAGA CTCTGTCCAG CCTTGTACAG ATGAGGTAGT AACAGGACGA 
GCTCTAGTGT GTCCTGTTTG TAACCTAGAA CAAGAGACTT CTGATCTTAC CCTCTTCAAC 
ATACATGTGG ATATTTGCTT AAATAAAGGT ATTATCCAAG AACTGAGAAA TAGTGAAGGT 
AATTCAGTTA AACAACCCAA AGAAAGCTCG AGAAGTACTG ACAGACTTCA GAAGGCTTCA 
GGAAGGACCA AAAGGCCAGG AACGAAGACA AAGAGCTCAA CTTTGAAGAA AACAAAGCCC 
CGAGATCCCA GACACACCCT TGATGGATTT TTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polk ( NM_001347602.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334531.1 Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant 2, mRNA.

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CloneID	OMu49059
Clone ID Related Accession (Same CDS sequence)	NM_001347602.1 , XM_030247285.1
Accession Version	NM_001347602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	DNA polymerase kappa isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK138299.1, AC122828.3, CN698108.1, DV656157.1, BG061549.2, BY707241.1, AK014087.1, BG060790.2 and EH097080.1. On Dec 2, 2016 this sequence version replaced XM_006517679.3. Transcript Variant: This variant (2) contains lacks an alternate exon but contains a different in-frame exon in the central coding region, compared to variant a, resulting in an isoform (2) that is shorter than isoform a. The 5' UTR of this variant is incomplete because there are no 5'-complete transcripts representing it and there is splicing ambiguity at the 5' end. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY769920.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164133 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATAACA CAAAGGAAAA GGACAACTTC AAAGACGACC TCCTGCTCCG CATGGGACTA 
AACGATAACA AAGCAGGCAT GGAAGGGTTG GATAAGGAGA AAATTAACAA AATTATCATG 
GAAGCCACAA AGGGGTCCAG ATTTTATGGA AATGAGCTCA AGAAGGAAAA GCAAGTCAAT 
CAACGGATTG AAAATATGAT GCAACAAAAA GCTCAAATTA CCAGCCAGCA ACTAAGGAAA 
GCTCAATTAC AGGTTGACAA ATTTGCAATG GAGTTAGAAC GGAACCGGAA TTTGAACAAT 
ACCATAGTTC ATGTTGACAT GGACGCTTTC TATGCAGCTG TGGAAATGAG GGACAACCCG 
GAACTGAAGG ATAAACCCAT TGCTGTAGGA TCCATGAGCA TGTTGGCTAC TTCGAATTAC 
CATGCAAGGA GGTTTGGTGT CCGTGCAGCC ATGCCAGGAT TTATTGCTAA GAGGCTCTGC 
CCACAACTTA TTATAGTGCC CCCAAACTTT GACAAATATA GAGCTGTGAG TAAGGAGGTT 
AAGGAGATAC TTGCTGAATA TGATCCCAAT TTTATGGCCA TGAGTCTGGA CGAAGCCTAC 
TTGAATATAA CACAGCACTT GCAGGAAAGG CAAGATTGGC CTGAGGACAA AAGAAGATAC 
TTCATCAAGA TGGGAAACTA CTTAAAAATC GCGATGATGA CATTGGATTT GGCTGCTAGT 
AACTTGAACC TACTGAGGCC AGCTAAACCT CCAGGCATCG CCCCCAATAC AATGTTAGCA 
AAAGTGTGCA GTGATAAGAA TAAGCCAAAC GGACAGTACC AGATCCTTCC CAGCAGGAGC 
GCGGTGATGG ACTTCATCAA GGACCTGCCT ATTAGAAAGG TTTCTGGGAT AGGAAAAGTT 
ACAGAGAAAA TGTTAATGGC TCTCGGGATT GTTACTTGCA CAGAACTCTA CCAACAGAGA 
GCGTTGCTGT CTCTCCTTTT CTCTGAAACC TCTTGGCATT ATTTTCTTCA CATCGCGCTG 
GGTCTAGGTT CAACAGACCT GGCAAGGGAT GGAGAAAGGA AAAGCATGAG TGTTGAAAGG 
ACATTCAGTG AGATAAGTAA GACAGAGGAA CAGTACAGCC TGTGCCAAGA ACTGTGCGCT 
GAGCTCGCCC ACGACCTCCA GAAGGAAGGA CTTAAGGGAA GAACCGTCAC CATTAAGCTG 
AAGAACGTGA ATTTTGAAGT AAAAACTCGT GCATCTACCG TTCCGGCCGC CATTTCTACT 
GCAGAGGAAA TATTTGCCAT TGCCAAGGAG CTGCTAAGGA CAGAAGTTAA TGTGGGTTCT 
CCACACCCCC TGCGGTTAAG ACTGATGGGT GTCCGAATGT CTACTTTTTC CAGTGAAGAT 
GACAGGAAAC ACCAACAAAG GAGCATCATT GGTTTCTTAC AAGCTGGAAA CCAAGCTTTG 
TCATCTACTG GGGATAGTCT AGACAAAACT GACAAAACTG AGCTTGCAAA GCCCTTAGAA 
ATGTCTCATA AGAAGAGTTT CTTTGATAAA AAGCGATCAG AAAGAATCTC CAACTGTCAA 
GACACATCCA GATGTAAAAC TGCGGGTCAG CAAGCTTTAC AGATCTTGGA ACCATCCCAA 
GCATTAAAGA AGCTGAGCGA GAGTTTTGAA ACATCAGAGA ATTCAAATGA CTGTCAGACA 
TTTATATGTC CAGTTTGCTT TAGGGAGCAA GAAGGTGTCA GTCTGGAAGC CTTTAATGAA 
CATGTAGATG AGTGTCTTGA TGGACCGTCA ACCAGTGAGA ACTCAAAAAT ATCCTGTTAC 
TCACATGCTT CCTCTGCAGA CATTGGTCAG AAGGAAGATG TACACCCCTC TATTCCACTG 
TGTGAGAAAC GGGGGCATGA AAATGGAGAG ATCACTTTAG TAGATGGTGT AGATCTAACA 
GGGACGGAAG ACAGATCATT GAAAGCAGCA AGGATGGACA CTCTAGAGAA TAATCGCAGC 
AAAGAGGAAT GTCCTGATAT TCCAGACAAG TCTTGTCCTA TATCACTGGA AAATGAAACC 
ATCAGTACAT TAAGTAGGCA AGACTCTGTC CAGCCTTGTA CAGATGAGGT AGTAACAGGA 
CGAGCTCTAG TGTGTCCTGT TTGTAACCTA GAACAAGAGA CTTCTGATCT TACCCTCTTC 
AACATACATG TGGATATTTG CTTAAATAAA GGTATTATCC AAGAACTGAG AAATAGTGAA 
GGTAATTCAG TTAAACAACC CAAAGAAAGC TCGAGAAGTA CTGACAGACT TCAGAAGGCT 
TCAGGAAGGA CCAAAAGGCC AGGAACGAAG ACAAAGAGCT CAACTTTGAA GAAAACAAAG 
CCCCGAGATC CCAGACACAC CCTTGATGGA TTTTTTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334531.1
CDS	28..2409
Translation	MDNTKEKDNFKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDKFAMELERNRNLNNTIVHVDMDAFYAAVEMRDNPELKDKPIAVGSMSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILAEYDPNFMAMSLDEAYLNITQHLQERQDWPEDKRRYFIKMGNYLKIAMMTLDLAASNLNLLRPAKPPGIAPNTMLAKVCSDKNKPNGQYQILPSRSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMSVERTFSEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLRTEVNVGSPHPLRLRLMGVRMSTFSSEDDRKHQQRSIIGFLQAGNQALSSTGDSLDKTDKTELAKPLEMSHKKSFFDKKRSERISNCQDTSRCKTAGQQALQILEPSQALKKLSESFETSENSNDCQTFICPVCFREQEGVSLEAFNEHVDECLDGPSTSENSKISCYSHASSADIGQKEDVHPSIPLCEKRGHENGEITLVDGVDLTGTEDRSLKAARMDTLENNRSKEECPDIPDKSCPISLENETISTLSRQDSVQPCTDEVVTGRALVCPVCNLEQETSDLTLFNIHVDICLNKGIIQELRNSEGNSVKQPKESSRSTDRLQKASGRTKRPGTKTKSSTLKKTKPRDPRHTLDGFFK

Target ORF information:

RefSeq Version	NM_001347602.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347602.1

ORF Insert Sequence:

ATGGATAACA CAAAGGAAAA GGACAACTTC AAAGACGACC TCCTGCTCCG CATGGGACTA 
AACGATAACA AAGCAGGCAT GGAAGGGTTG GATAAGGAGA AAATTAACAA AATTATCATG 
GAAGCCACAA AGGGGTCCAG ATTTTATGGA AATGAGCTCA AGAAGGAAAA GCAAGTCAAT 
CAACGGATTG AAAATATGAT GCAACAAAAA GCTCAAATTA CCAGCCAGCA ACTAAGGAAA 
GCTCAATTAC AGGTTGACAA ATTTGCAATG GAGTTAGAAC GGAACCGGAA TTTGAACAAT 
ACCATAGTTC ATGTTGACAT GGACGCTTTC TATGCAGCTG TGGAAATGAG GGACAACCCG 
GAACTGAAGG ATAAACCCAT TGCTGTAGGA TCCATGAGCA TGTTGGCTAC TTCGAATTAC 
CATGCAAGGA GGTTTGGTGT CCGTGCAGCC ATGCCAGGAT TTATTGCTAA GAGGCTCTGC 
CCACAACTTA TTATAGTGCC CCCAAACTTT GACAAATATA GAGCTGTGAG TAAGGAGGTT 
AAGGAGATAC TTGCTGAATA TGATCCCAAT TTTATGGCCA TGAGTCTGGA CGAAGCCTAC 
TTGAATATAA CACAGCACTT GCAGGAAAGG CAAGATTGGC CTGAGGACAA AAGAAGATAC 
TTCATCAAGA TGGGAAACTA CTTAAAAATC GCGATGATGA CATTGGATTT GGCTGCTAGT 
AACTTGAACC TACTGAGGCC AGCTAAACCT CCAGGCATCG CCCCCAATAC AATGTTAGCA 
AAAGTGTGCA GTGATAAGAA TAAGCCAAAC GGACAGTACC AGATCCTTCC CAGCAGGAGC 
GCGGTGATGG ACTTCATCAA GGACCTGCCT ATTAGAAAGG TTTCTGGGAT AGGAAAAGTT 
ACAGAGAAAA TGTTAATGGC TCTCGGGATT GTTACTTGCA CAGAACTCTA CCAACAGAGA 
GCGTTGCTGT CTCTCCTTTT CTCTGAAACC TCTTGGCATT ATTTTCTTCA CATCGCGCTG 
GGTCTAGGTT CAACAGACCT GGCAAGGGAT GGAGAAAGGA AAAGCATGAG TGTTGAAAGG 
ACATTCAGTG AGATAAGTAA GACAGAGGAA CAGTACAGCC TGTGCCAAGA ACTGTGCGCT 
GAGCTCGCCC ACGACCTCCA GAAGGAAGGA CTTAAGGGAA GAACCGTCAC CATTAAGCTG 
AAGAACGTGA ATTTTGAAGT AAAAACTCGT GCATCTACCG TTCCGGCCGC CATTTCTACT 
GCAGAGGAAA TATTTGCCAT TGCCAAGGAG CTGCTAAGGA CAGAAGTTAA TGTGGGTTCT 
CCACACCCCC TGCGGTTAAG ACTGATGGGT GTCCGAATGT CTACTTTTTC CAGTGAAGAT 
GACAGGAAAC ACCAACAAAG GAGCATCATT GGTTTCTTAC AAGCTGGAAA CCAAGCTTTG 
TCATCTACTG GGGATAGTCT AGACAAAACT GACAAAACTG AGCTTGCAAA GCCCTTAGAA 
ATGTCTCATA AGAAGAGTTT CTTTGATAAA AAGCGATCAG AAAGAATCTC CAACTGTCAA 
GACACATCCA GATGTAAAAC TGCGGGTCAG CAAGCTTTAC AGATCTTGGA ACCATCCCAA 
GCATTAAAGA AGCTGAGCGA GAGTTTTGAA ACATCAGAGA ATTCAAATGA CTGTCAGACA 
TTTATATGTC CAGTTTGCTT TAGGGAGCAA GAAGGTGTCA GTCTGGAAGC CTTTAATGAA 
CATGTAGATG AGTGTCTTGA TGGACCGTCA ACCAGTGAGA ACTCAAAAAT ATCCTGTTAC 
TCACATGCTT CCTCTGCAGA CATTGGTCAG AAGGAAGATG TACACCCCTC TATTCCACTG 
TGTGAGAAAC GGGGGCATGA AAATGGAGAG ATCACTTTAG TAGATGGTGT AGATCTAACA 
GGGACGGAAG ACAGATCATT GAAAGCAGCA AGGATGGACA CTCTAGAGAA TAATCGCAGC 
AAAGAGGAAT GTCCTGATAT TCCAGACAAG TCTTGTCCTA TATCACTGGA AAATGAAACC 
ATCAGTACAT TAAGTAGGCA AGACTCTGTC CAGCCTTGTA CAGATGAGGT AGTAACAGGA 
CGAGCTCTAG TGTGTCCTGT TTGTAACCTA GAACAAGAGA CTTCTGATCT TACCCTCTTC 
AACATACATG TGGATATTTG CTTAAATAAA GGTATTATCC AAGAACTGAG AAATAGTGAA 
GGTAATTCAG TTAAACAACC CAAAGAAAGC TCGAGAAGTA CTGACAGACT TCAGAAGGCT 
TCAGGAAGGA CCAAAAGGCC AGGAACGAAG ACAAAGAGCT CAACTTTGAA GAAAACAAAG 
CCCCGAGATC CCAGACACAC CCTTGATGGA TTTTTTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polk ( XM_006517680.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517743.1 Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu49060
Clone ID Related Accession (Same CDS sequence)	XM_006517680.4 , XM_006517680.3
Accession Version	XM_006517680.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA polymerase kappa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517680.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAC AAAAAGCTCA AATTACCAGC CAGCAACTAA GGAAAGCTCA ATTACAGGTT 
GACAAATTTG CAATGGAGTT AGAACGGAAC CGGAATTTGA ACAATACCAT AGTTCATGTT 
GACATGGACG CTTTCTATGC AGCTGTGGAA ATGAGGGACA ACCCGGAACT GAAGGATAAA 
CCCATTGCTG TAGGATCCAT GAGCATGTTG GCTACTTCGA ATTACCATGC AAGGAGGTTT 
GGTGTCCGTG CAGCCATGCC AGGATTTATT GCTAAGAGGC TCTGCCCACA ACTTATTATA 
GTGCCCCCAA ACTTTGACAA ATATAGAGCT GTGAGTAAGG AGGTTAAGGA GATACTTGCT 
GAATATGATC CCAATTTTAT GGCCATGAGT CTGGACGAAG CCTACTTGAA TATAACACAG 
CACTTGCAGG AAAGGCAAGA TTGGCCTGAG GACAAAAGAA GATACTTCAT CAAGATGGGA 
AACTACTTAA AAATCGACAC ACCCAGACAG GAAGCTAACG AGCTGACTGA GTATGAGCGG 
TCCATCTCCC CGCTGCTTTT TGAAGATAGT CCTCCTGATT TGCAACCCCA AGGAAGTCCT 
TTCCAACTGA ACTCTGAAGA ACAAAACAAT CCTCAAATAG CCCAAAATTC AGTTGTTTTT 
GGAACATCAG CTGAGGAAGT GGTAAAGGAA ATTCGCTTCA GAATTGAACA AAAAACAACG 
CTGACAGCCA GCGCAGGCAT CGCCCCCAAT ACAATGTTAG CAAAAGTGTG CAGTGATAAG 
AATAAGCCAA ACGGACAGTA CCAGATCCTT CCCAGCAGGA GCGCGGTGAT GGACTTCATC 
AAGGACCTGC CTATTAGAAA GGTTTCTGGG ATAGGAAAAG TTACAGAGAA AATGTTAATG 
GCTCTCGGGA TTGTTACTTG CACAGAACTC TACCAACAGA GAGCGTTGCT GTCTCTCCTT 
TTCTCTGAAA CCTCTTGGCA TTATTTTCTT CACATCGCGC TGGGTCTAGG TTCAACAGAC 
CTGGCAAGGG ATGGAGAAAG GAAAAGCATG AGTGTTGAAA GGACATTCAG TGAGATAAGT 
AAGACAGAGG AACAGTACAG CCTGTGCCAA GAACTGTGCG CTGAGCTCGC CCACGACCTC 
CAGAAGGAAG GACTTAAGGG AAGAACCGTC ACCATTAAGC TGAAGAACGT GAATTTTGAA 
GTAAAAACTC GTGCATCTAC CGTTCCGGCC GCCATTTCTA CTGCAGAGGA AATATTTGCC 
ATTGCCAAGG AGCTGCTAAG GACAGAAGTT AATGTGGGTT CTCCACACCC CCTGCGGTTA 
AGACTGATGG GTGTCCGAAT GTCTACTTTT TCCAGTGAAG ATGACAGGAA ACACCAACAA 
AGGAGCATCA TTGGTTTCTT ACAAGCTGGA AACCAAGCTT TGTCATCTAC TGGGGATAGT 
CTAGACAAAA CTGACAAAAC TGAGCTTGCA AAGCCCTTAG AAATGTCTCA TAAGAAGAGT 
TTCTTTGATA AAAAGCGATC AGAAAGAATC TCCAACTGTC AAGACACATC CAGATGTAAA 
ACTGCGGGTC AGCAAGCTTT ACAGATCTTG GAACCATCCC AAGCATTAAA GAAGCTGAGC 
GAGAGTTTTG AAACATCAGA GAATTCAAAT GACTGTCAGA CATTTATATG TCCAGTTTGC 
TTTAGGGAGC AAGAAGGTGT CAGTCTGGAA GCCTTTAATG AACATGTAGA TGAGTGTCTT 
GATGGACCGT CAACCAGTGA GAACTCAAAA ATATCCTGTT ACTCACATGC TTCCTCTGCA 
GACATTGGTC AGAAGGAAGA TGTACACCCC TCTATTCCAC TGTGTGAGAA ACGGGGGCAT 
GAAAATGGAG AGATCACTTT AGTAGATGGT GTAGATCTAA CAGGGACGGA AGACAGATCA 
TTGAAAGCAG CAAGGATGGA CACTCTAGAG AATAATCGCA GCAAAGAGGA ATGTCCTGAT 
ATTCCAGACA AGTCTTGTCC TATATCACTG GAAAATGAAA CCATCAGTAC ATTAAGTAGG 
CAAGACTCTG TCCAGCCTTG TACAGATGAG GTAGTAACAG GACGAGCTCT AGTGTGTCCT 
GTTTGTAACC TAGAACAAGA GACTTCTGAT CTTACCCTCT TCAACATACA TGTGGATATT 
TGCTTAAATA AAGGTATTAT CCAAGAACTG AGAAATAGTG AAGGTAATTC AGTTAAACAA 
CCCAAAGAAA GCTCGAGAAG TACTGACAGA CTTCAGAAGG CTTCAGGAAG GACCAAAAGG 
CCAGGAACGA AGACAAAGAG CTCAACTTTG AAGAAAACAA AGCCCCGAGA TCCCAGACAC 
ACCCTTGATG GATTTTTTAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517743.1
CDS	381..2744
Translation	MMQQKAQITSQQLRKAQLQVDKFAMELERNRNLNNTIVHVDMDAFYAAVEMRDNPELKDKPIAVGSMSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILAEYDPNFMAMSLDEAYLNITQHLQERQDWPEDKRRYFIKMGNYLKIDTPRQEANELTEYERSISPLLFEDSPPDLQPQGSPFQLNSEEQNNPQIAQNSVVFGTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSRSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMSVERTFSEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLRTEVNVGSPHPLRLRLMGVRMSTFSSEDDRKHQQRSIIGFLQAGNQALSSTGDSLDKTDKTELAKPLEMSHKKSFFDKKRSERISNCQDTSRCKTAGQQALQILEPSQALKKLSESFETSENSNDCQTFICPVCFREQEGVSLEAFNEHVDECLDGPSTSENSKISCYSHASSADIGQKEDVHPSIPLCEKRGHENGEITLVDGVDLTGTEDRSLKAARMDTLENNRSKEECPDIPDKSCPISLENETISTLSRQDSVQPCTDEVVTGRALVCPVCNLEQETSDLTLFNIHVDICLNKGIIQELRNSEGNSVKQPKESSRSTDRLQKASGRTKRPGTKTKSSTLKKTKPRDPRHTLDGFFK

Target ORF information:

RefSeq Version	XM_006517680.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517680.3

ORF Insert Sequence:

ATGATGCAAC AAAAAGCTCA AATTACCAGC CAGCAACTAA GGAAAGCTCA ATTACAGGTT 
GACAAATTTG CAATGGAGTT AGAACGGAAC CGGAATTTGA ACAATACCAT AGTTCATGTT 
GACATGGACG CTTTCTATGC AGCTGTGGAA ATGAGGGACA ACCCGGAACT GAAGGATAAA 
CCCATTGCTG TAGGATCCAT GAGCATGTTG GCTACTTCGA ATTACCATGC AAGGAGGTTT 
GGTGTCCGTG CAGCCATGCC AGGATTTATT GCTAAGAGGC TCTGCCCACA ACTTATTATA 
GTGCCCCCAA ACTTTGACAA ATATAGAGCT GTGAGTAAGG AGGTTAAGGA GATACTTGCT 
GAATATGATC CCAATTTTAT GGCCATGAGT CTGGACGAAG CCTACTTGAA TATAACACAG 
CACTTGCAGG AAAGGCAAGA TTGGCCTGAG GACAAAAGAA GATACTTCAT CAAGATGGGA 
AACTACTTAA AAATCGACAC ACCCAGACAG GAAGCTAACG AGCTGACTGA GTATGAGCGG 
TCCATCTCCC CGCTGCTTTT TGAAGATAGT CCTCCTGATT TGCAACCCCA AGGAAGTCCT 
TTCCAACTGA ACTCTGAAGA ACAAAACAAT CCTCAAATAG CCCAAAATTC AGTTGTTTTT 
GGAACATCAG CTGAGGAAGT GGTAAAGGAA ATTCGCTTCA GAATTGAACA AAAAACAACG 
CTGACAGCCA GCGCAGGCAT CGCCCCCAAT ACAATGTTAG CAAAAGTGTG CAGTGATAAG 
AATAAGCCAA ACGGACAGTA CCAGATCCTT CCCAGCAGGA GCGCGGTGAT GGACTTCATC 
AAGGACCTGC CTATTAGAAA GGTTTCTGGG ATAGGAAAAG TTACAGAGAA AATGTTAATG 
GCTCTCGGGA TTGTTACTTG CACAGAACTC TACCAACAGA GAGCGTTGCT GTCTCTCCTT 
TTCTCTGAAA CCTCTTGGCA TTATTTTCTT CACATCGCGC TGGGTCTAGG TTCAACAGAC 
CTGGCAAGGG ATGGAGAAAG GAAAAGCATG AGTGTTGAAA GGACATTCAG TGAGATAAGT 
AAGACAGAGG AACAGTACAG CCTGTGCCAA GAACTGTGCG CTGAGCTCGC CCACGACCTC 
CAGAAGGAAG GACTTAAGGG AAGAACCGTC ACCATTAAGC TGAAGAACGT GAATTTTGAA 
GTAAAAACTC GTGCATCTAC CGTTCCGGCC GCCATTTCTA CTGCAGAGGA AATATTTGCC 
ATTGCCAAGG AGCTGCTAAG GACAGAAGTT AATGTGGGTT CTCCACACCC CCTGCGGTTA 
AGACTGATGG GTGTCCGAAT GTCTACTTTT TCCAGTGAAG ATGACAGGAA ACACCAACAA 
AGGAGCATCA TTGGTTTCTT ACAAGCTGGA AACCAAGCTT TGTCATCTAC TGGGGATAGT 
CTAGACAAAA CTGACAAAAC TGAGCTTGCA AAGCCCTTAG AAATGTCTCA TAAGAAGAGT 
TTCTTTGATA AAAAGCGATC AGAAAGAATC TCCAACTGTC AAGACACATC CAGATGTAAA 
ACTGCGGGTC AGCAAGCTTT ACAGATCTTG GAACCATCCC AAGCATTAAA GAAGCTGAGC 
GAGAGTTTTG AAACATCAGA GAATTCAAAT GACTGTCAGA CATTTATATG TCCAGTTTGC 
TTTAGGGAGC AAGAAGGTGT CAGTCTGGAA GCCTTTAATG AACATGTAGA TGAGTGTCTT 
GATGGACCGT CAACCAGTGA GAACTCAAAA ATATCCTGTT ACTCACATGC TTCCTCTGCA 
GACATTGGTC AGAAGGAAGA TGTACACCCC TCTATTCCAC TGTGTGAGAA ACGGGGGCAT 
GAAAATGGAG AGATCACTTT AGTAGATGGT GTAGATCTAA CAGGGACGGA AGACAGATCA 
TTGAAAGCAG CAAGGATGGA CACTCTAGAG AATAATCGCA GCAAAGAGGA ATGTCCTGAT 
ATTCCAGACA AGTCTTGTCC TATATCACTG GAAAATGAAA CCATCAGTAC ATTAAGTAGG 
CAAGACTCTG TCCAGCCTTG TACAGATGAG GTAGTAACAG GACGAGCTCT AGTGTGTCCT 
GTTTGTAACC TAGAACAAGA GACTTCTGAT CTTACCCTCT TCAACATACA TGTGGATATT 
TGCTTAAATA AAGGTATTAT CCAAGAACTG AGAAATAGTG AAGGTAATTC AGTTAAACAA 
CCCAAAGAAA GCTCGAGAAG TACTGACAGA CTTCAGAAGG CTTCAGGAAG GACCAAAAGG 
CCAGGAACGA AGACAAAGAG CTCAACTTTG AAGAAAACAA AGCCCCGAGA TCCCAGACAC 
ACCCTTGATG GATTTTTTAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polk ( NM_001347604.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334533.1 Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OMu83385
Clone ID Related Accession (Same CDS sequence)	NM_001347604.1
Accession Version	NM_001347604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	DNA polymerase kappa isoform 8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK138299.1, CN698108.1, DV656157.1, BG061549.2, AK014087.1, BG060790.2 and EH097080.1. Transcript Variant: This variant (8) contains lacks an alternate in-frame exon in the 3' coding region, compared to variant a, resulting in an isoform (8) that is shorter than isoform a. The 5' UTR of this variant is incomplete because there are no 5'-complete transcripts representing it and there is splicing ambiguity at the 5' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY769926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATAACA CAAAGGAAAA GGACAACTTC AAAGACGACC TCCTGCTCCG CATGGGACTA 
AACGATAACA AAGCAGGCAT GGAAGGGTTG GATAAGGAGA AAATTAACAA AATTATCATG 
GAAGCCACAA AGGGGTCCAG ATTTTATGGA AATGAGCTCA AGAAGGAAAA GCAAGTCAAT 
CAACGGATTG AAAATATGAT GCAACAAAAA GCTCAAATTA CCAGCCAGCA ACTAAGGAAA 
GCTCAATTAC AGGTTGACAA ATTTGCAATG GAGTTAGAAC GGAACCGGAA TTTGAACAAT 
ACCATAGTTC ATGTTGACAT GGACGCTTTC TATGCAGCTG TGGAAATGAG GGACAACCCG 
GAACTGAAGG ATAAACCCAT TGCTGTAGGA TCCATGAGCA TGTTGGCTAC TTCGAATTAC 
CATGCAAGGA GGTTTGGTGT CCGTGCAGCC ATGCCAGGAT TTATTGCTAA GAGGCTCTGC 
CCACAACTTA TTATAGTGCC CCCAAACTTT GACAAATATA GAGCTGTGAG TAAGGAGGTT 
AAGGAGATAC TTGCTGAATA TGATCCCAAT TTTATGGCCA TGAGTCTGGA CGAAGCCTAC 
TTGAATATAA CACAGCACTT GCAGGAAAGG CAAGATTGGC CTGAGGACAA AAGAAGATAC 
TTCATCAAGA TGGGAAACTA CTTAAAAATC GACACACCCA GACAGGAAGC TAACGAGCTG 
ACTGAGTATG AGCGGTCCAT CTCCCCGCTG CTTTTTGAAG ATAGTCCTCC TGATTTGCAA 
CCCCAAGGAA GTCCTTTCCA ACTGAACTCT GAAGAACAAA ACAATCCTCA AATAGCCCAA 
AATTCAGTTG TTTTTGGAAC ATCAGCTGAG GAAGTGGTAA AGGAAATTCG CTTCAGAATT 
GAACAAAAAA CAACGCTGAC AGCCAGCGCA GGCATCGCCC CCAATACAAT GTTAGCAAAA 
GTGTGCAGTG ATAAGAATAA GCCAAACGGA CAGTACCAGA TCCTTCCCAG CAGGAGCGCG 
GTGATGGACT TCATCAAGGA CCTGCCTATT AGAAAGGTTT CTGGGATAGG AAAAGTTACA 
GAGAAAATGT TAATGGCTCT CGGGATTGTT ACTTGCACAG AACTCTACCA ACAGAGAGCG 
TTGCTGTCTC TCCTTTTCTC TGAAACCTCT TGGCATTATT TTCTTCACAT CGCGCTGGGT 
CTAGGTTCAA CAGACCTGGC AAGGGATGGA GAAAGGAAAA GCATGAGTGT TGAAAGGACA 
TTCAGTGAGA TAAGTAAGAC AGAGGAACAG TACAGCCTGT GCCAAGAACT GTGCGCTGAG 
CTCGCCCACG ACCTCCAGAA GGAAGGACTT AAGGGAAGAA CCGTCACCAT TAAGCTGAAG 
AACGTGAATT TTGAAGTAAA AACTCGTGCA TCTACCGTTC CGGCCGCCAT TTCTACTGCA 
GAGGAAATAT TTGCCATTGC CAAGGAGCTG CTAAGGACAG AAGTTAATGT GGGTTCTCCA 
CACCCCCTGC GGTTAAGACT GATGGACAGA CTTCAGAAGG CTTCAGGAAG GACCAAAAGG 
CCAGGAACGA AGACAAAGAG CTCAACTTTG AAGAAAACAA AGCCCCGAGA TCCCAGACAC 
ACCCTTGATG GATTTTTTAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334533.1
CDS	28..1671
Translation	MDNTKEKDNFKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDKFAMELERNRNLNNTIVHVDMDAFYAAVEMRDNPELKDKPIAVGSMSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILAEYDPNFMAMSLDEAYLNITQHLQERQDWPEDKRRYFIKMGNYLKIDTPRQEANELTEYERSISPLLFEDSPPDLQPQGSPFQLNSEEQNNPQIAQNSVVFGTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSRSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMSVERTFSEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLRTEVNVGSPHPLRLRLMDRLQKASGRTKRPGTKTKSSTLKKTKPRDPRHTLDGFFK

Target ORF information:

RefSeq Version	NM_001347604.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347604.1

ORF Insert Sequence:

ATGGATAACA CAAAGGAAAA GGACAACTTC AAAGACGACC TCCTGCTCCG CATGGGACTA 
AACGATAACA AAGCAGGCAT GGAAGGGTTG GATAAGGAGA AAATTAACAA AATTATCATG 
GAAGCCACAA AGGGGTCCAG ATTTTATGGA AATGAGCTCA AGAAGGAAAA GCAAGTCAAT 
CAACGGATTG AAAATATGAT GCAACAAAAA GCTCAAATTA CCAGCCAGCA ACTAAGGAAA 
GCTCAATTAC AGGTTGACAA ATTTGCAATG GAGTTAGAAC GGAACCGGAA TTTGAACAAT 
ACCATAGTTC ATGTTGACAT GGACGCTTTC TATGCAGCTG TGGAAATGAG GGACAACCCG 
GAACTGAAGG ATAAACCCAT TGCTGTAGGA TCCATGAGCA TGTTGGCTAC TTCGAATTAC 
CATGCAAGGA GGTTTGGTGT CCGTGCAGCC ATGCCAGGAT TTATTGCTAA GAGGCTCTGC 
CCACAACTTA TTATAGTGCC CCCAAACTTT GACAAATATA GAGCTGTGAG TAAGGAGGTT 
AAGGAGATAC TTGCTGAATA TGATCCCAAT TTTATGGCCA TGAGTCTGGA CGAAGCCTAC 
TTGAATATAA CACAGCACTT GCAGGAAAGG CAAGATTGGC CTGAGGACAA AAGAAGATAC 
TTCATCAAGA TGGGAAACTA CTTAAAAATC GACACACCCA GACAGGAAGC TAACGAGCTG 
ACTGAGTATG AGCGGTCCAT CTCCCCGCTG CTTTTTGAAG ATAGTCCTCC TGATTTGCAA 
CCCCAAGGAA GTCCTTTCCA ACTGAACTCT GAAGAACAAA ACAATCCTCA AATAGCCCAA 
AATTCAGTTG TTTTTGGAAC ATCAGCTGAG GAAGTGGTAA AGGAAATTCG CTTCAGAATT 
GAACAAAAAA CAACGCTGAC AGCCAGCGCA GGCATCGCCC CCAATACAAT GTTAGCAAAA 
GTGTGCAGTG ATAAGAATAA GCCAAACGGA CAGTACCAGA TCCTTCCCAG CAGGAGCGCG 
GTGATGGACT TCATCAAGGA CCTGCCTATT AGAAAGGTTT CTGGGATAGG AAAAGTTACA 
GAGAAAATGT TAATGGCTCT CGGGATTGTT ACTTGCACAG AACTCTACCA ACAGAGAGCG 
TTGCTGTCTC TCCTTTTCTC TGAAACCTCT TGGCATTATT TTCTTCACAT CGCGCTGGGT 
CTAGGTTCAA CAGACCTGGC AAGGGATGGA GAAAGGAAAA GCATGAGTGT TGAAAGGACA 
TTCAGTGAGA TAAGTAAGAC AGAGGAACAG TACAGCCTGT GCCAAGAACT GTGCGCTGAG 
CTCGCCCACG ACCTCCAGAA GGAAGGACTT AAGGGAAGAA CCGTCACCAT TAAGCTGAAG 
AACGTGAATT TTGAAGTAAA AACTCGTGCA TCTACCGTTC CGGCCGCCAT TTCTACTGCA 
GAGGAAATAT TTGCCATTGC CAAGGAGCTG CTAAGGACAG AAGTTAATGT GGGTTCTCCA 
CACCCCCTGC GGTTAAGACT GATGGACAGA CTTCAGAAGG CTTCAGGAAG GACCAAAAGG 
CCAGGAACGA AGACAAAGAG CTCAACTTTG AAGAAAACAA AGCCCCGAGA TCCCAGACAC 
ACCCTTGATG GATTTTTTAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polk ( NM_001347603.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334532.1 Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant 3, mRNA.

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CloneID	OMu83387
Clone ID Related Accession (Same CDS sequence)	NM_001347603.1
Accession Version	NM_001347603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	DNA polymerase kappa isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK138299.1, CN698108.1, DV656157.1, BG061549.2, BY707241.1, AK014087.1, BG060790.2 and EH097080.1. Transcript Variant: This variant (3) contains lacks an alternate in-frame exon in the central coding region, compared to variant a, resulting in an isoform (3) that is shorter than isoform a. The 5' UTR of this variant is incomplete because there are no 5'-complete transcripts representing it and there is splicing ambiguity at the 5' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY769921.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164133 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATAACA CAAAGGAAAA GGACAACTTC AAAGACGACC TCCTGCTCCG CATGGGACTA 
AACGATAACA AAGCAGGCAT GGAAGGGTTG GATAAGGAGA AAATTAACAA AATTATCATG 
GAAGCCACAA AGGGGTCCAG ATTTTATGGA AATGAGCTCA AGAAGGAAAA GCAAGTCAAT 
CAACGGATTG AAAATATGAT GCAACAAAAA GCTCAAATTA CCAGCCAGCA ACTAAGGAAA 
GCTCAATTAC AGGTTGACAA ATTTGCAATG GAGTTAGAAC GGAACCGGAA TTTGAACAAT 
ACCATAGTTC ATGTTGACAT GGACGCTTTC TATGCAGCTG TGGAAATGAG GGACAACCCG 
GAACTGAAGG ATAAACCCAT TGCTGTAGGA TCCATGAGCA TGTTGGCTAC TTCGAATTAC 
CATGCAAGGA GGTTTGGTGT CCGTGCAGCC ATGCCAGGAT TTATTGCTAA GAGGCTCTGC 
CCACAACTTA TTATAGTGCC CCCAAACTTT GACAAATATA GAGCTGTGAG TAAGGAGGTT 
AAGGAGATAC TTGCTGAATA TGATCCCAAT TTTATGGCCA TGAGTCTGGA CGAAGCCTAC 
TTGAATATAA CACAGCACTT GCAGGAAAGG CAAGATTGGC CTGAGGACAA AAGAAGATAC 
TTCATCAAGA TGGGAAACTA CTTAAAAATC GGCATCGCCC CCAATACAAT GTTAGCAAAA 
GTGTGCAGTG ATAAGAATAA GCCAAACGGA CAGTACCAGA TCCTTCCCAG CAGGAGCGCG 
GTGATGGACT TCATCAAGGA CCTGCCTATT AGAAAGGTTT CTGGGATAGG AAAAGTTACA 
GAGAAAATGT TAATGGCTCT CGGGATTGTT ACTTGCACAG AACTCTACCA ACAGAGAGCG 
TTGCTGTCTC TCCTTTTCTC TGAAACCTCT TGGCATTATT TTCTTCACAT CGCGCTGGGT 
CTAGGTTCAA CAGACCTGGC AAGGGATGGA GAAAGGAAAA GCATGAGTGT TGAAAGGACA 
TTCAGTGAGA TAAGTAAGAC AGAGGAACAG TACAGCCTGT GCCAAGAACT GTGCGCTGAG 
CTCGCCCACG ACCTCCAGAA GGAAGGACTT AAGGGAAGAA CCGTCACCAT TAAGCTGAAG 
AACGTGAATT TTGAAGTAAA AACTCGTGCA TCTACCGTTC CGGCCGCCAT TTCTACTGCA 
GAGGAAATAT TTGCCATTGC CAAGGAGCTG CTAAGGACAG AAGTTAATGT GGGTTCTCCA 
CACCCCCTGC GGTTAAGACT GATGGGTGTC CGAATGTCTA CTTTTTCCAG TGAAGATGAC 
AGGAAACACC AACAAAGGAG CATCATTGGT TTCTTACAAG CTGGAAACCA AGCTTTGTCA 
TCTACTGGGG ATAGTCTAGA CAAAACTGAC AAAACTGAGC TTGCAAAGCC CTTAGAAATG 
TCTCATAAGA AGAGTTTCTT TGATAAAAAG CGATCAGAAA GAATCTCCAA CTGTCAAGAC 
ACATCCAGAT GTAAAACTGC GGGTCAGCAA GCTTTACAGA TCTTGGAACC ATCCCAAGCA 
TTAAAGAAGC TGAGCGAGAG TTTTGAAACA TCAGAGAATT CAAATGACTG TCAGACATTT 
ATATGTCCAG TTTGCTTTAG GGAGCAAGAA GGTGTCAGTC TGGAAGCCTT TAATGAACAT 
GTAGATGAGT GTCTTGATGG ACCGTCAACC AGTGAGAACT CAAAAATATC CTGTTACTCA 
CATGCTTCCT CTGCAGACAT TGGTCAGAAG GAAGATGTAC ACCCCTCTAT TCCACTGTGT 
GAGAAACGGG GGCATGAAAA TGGAGAGATC ACTTTAGTAG ATGGTGTAGA TCTAACAGGG 
ACGGAAGACA GATCATTGAA AGCAGCAAGG ATGGACACTC TAGAGAATAA TCGCAGCAAA 
GAGGAATGTC CTGATATTCC AGACAAGTCT TGTCCTATAT CACTGGAAAA TGAAACCATC 
AGTACATTAA GTAGGCAAGA CTCTGTCCAG CCTTGTACAG ATGAGGTAGT AACAGGACGA 
GCTCTAGTGT GTCCTGTTTG TAACCTAGAA CAAGAGACTT CTGATCTTAC CCTCTTCAAC 
ATACATGTGG ATATTTGCTT AAATAAAGGT ATTATCCAAG AACTGAGAAA TAGTGAAGGT 
AATTCAGTTA AACAACCCAA AGAAAGCTCG AGAAGTACTG ACAGACTTCA GAAGGCTTCA 
GGAAGGACCA AAAGGCCAGG AACGAAGACA AAGAGCTCAA CTTTGAAGAA AACAAAGCCC 
CGAGATCCCA GACACACCCT TGATGGATTT TTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334532.1
CDS	28..2346
Translation	MDNTKEKDNFKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDKFAMELERNRNLNNTIVHVDMDAFYAAVEMRDNPELKDKPIAVGSMSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILAEYDPNFMAMSLDEAYLNITQHLQERQDWPEDKRRYFIKMGNYLKIGIAPNTMLAKVCSDKNKPNGQYQILPSRSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMSVERTFSEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLRTEVNVGSPHPLRLRLMGVRMSTFSSEDDRKHQQRSIIGFLQAGNQALSSTGDSLDKTDKTELAKPLEMSHKKSFFDKKRSERISNCQDTSRCKTAGQQALQILEPSQALKKLSESFETSENSNDCQTFICPVCFREQEGVSLEAFNEHVDECLDGPSTSENSKISCYSHASSADIGQKEDVHPSIPLCEKRGHENGEITLVDGVDLTGTEDRSLKAARMDTLENNRSKEECPDIPDKSCPISLENETISTLSRQDSVQPCTDEVVTGRALVCPVCNLEQETSDLTLFNIHVDICLNKGIIQELRNSEGNSVKQPKESSRSTDRLQKASGRTKRPGTKTKSSTLKKTKPRDPRHTLDGFFK

Target ORF information:

RefSeq Version	NM_001347603.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347603.1

ORF Insert Sequence:

ATGGATAACA CAAAGGAAAA GGACAACTTC AAAGACGACC TCCTGCTCCG CATGGGACTA 
AACGATAACA AAGCAGGCAT GGAAGGGTTG GATAAGGAGA AAATTAACAA AATTATCATG 
GAAGCCACAA AGGGGTCCAG ATTTTATGGA AATGAGCTCA AGAAGGAAAA GCAAGTCAAT 
CAACGGATTG AAAATATGAT GCAACAAAAA GCTCAAATTA CCAGCCAGCA ACTAAGGAAA 
GCTCAATTAC AGGTTGACAA ATTTGCAATG GAGTTAGAAC GGAACCGGAA TTTGAACAAT 
ACCATAGTTC ATGTTGACAT GGACGCTTTC TATGCAGCTG TGGAAATGAG GGACAACCCG 
GAACTGAAGG ATAAACCCAT TGCTGTAGGA TCCATGAGCA TGTTGGCTAC TTCGAATTAC 
CATGCAAGGA GGTTTGGTGT CCGTGCAGCC ATGCCAGGAT TTATTGCTAA GAGGCTCTGC 
CCACAACTTA TTATAGTGCC CCCAAACTTT GACAAATATA GAGCTGTGAG TAAGGAGGTT 
AAGGAGATAC TTGCTGAATA TGATCCCAAT TTTATGGCCA TGAGTCTGGA CGAAGCCTAC 
TTGAATATAA CACAGCACTT GCAGGAAAGG CAAGATTGGC CTGAGGACAA AAGAAGATAC 
TTCATCAAGA TGGGAAACTA CTTAAAAATC GGCATCGCCC CCAATACAAT GTTAGCAAAA 
GTGTGCAGTG ATAAGAATAA GCCAAACGGA CAGTACCAGA TCCTTCCCAG CAGGAGCGCG 
GTGATGGACT TCATCAAGGA CCTGCCTATT AGAAAGGTTT CTGGGATAGG AAAAGTTACA 
GAGAAAATGT TAATGGCTCT CGGGATTGTT ACTTGCACAG AACTCTACCA ACAGAGAGCG 
TTGCTGTCTC TCCTTTTCTC TGAAACCTCT TGGCATTATT TTCTTCACAT CGCGCTGGGT 
CTAGGTTCAA CAGACCTGGC AAGGGATGGA GAAAGGAAAA GCATGAGTGT TGAAAGGACA 
TTCAGTGAGA TAAGTAAGAC AGAGGAACAG TACAGCCTGT GCCAAGAACT GTGCGCTGAG 
CTCGCCCACG ACCTCCAGAA GGAAGGACTT AAGGGAAGAA CCGTCACCAT TAAGCTGAAG 
AACGTGAATT TTGAAGTAAA AACTCGTGCA TCTACCGTTC CGGCCGCCAT TTCTACTGCA 
GAGGAAATAT TTGCCATTGC CAAGGAGCTG CTAAGGACAG AAGTTAATGT GGGTTCTCCA 
CACCCCCTGC GGTTAAGACT GATGGGTGTC CGAATGTCTA CTTTTTCCAG TGAAGATGAC 
AGGAAACACC AACAAAGGAG CATCATTGGT TTCTTACAAG CTGGAAACCA AGCTTTGTCA 
TCTACTGGGG ATAGTCTAGA CAAAACTGAC AAAACTGAGC TTGCAAAGCC CTTAGAAATG 
TCTCATAAGA AGAGTTTCTT TGATAAAAAG CGATCAGAAA GAATCTCCAA CTGTCAAGAC 
ACATCCAGAT GTAAAACTGC GGGTCAGCAA GCTTTACAGA TCTTGGAACC ATCCCAAGCA 
TTAAAGAAGC TGAGCGAGAG TTTTGAAACA TCAGAGAATT CAAATGACTG TCAGACATTT 
ATATGTCCAG TTTGCTTTAG GGAGCAAGAA GGTGTCAGTC TGGAAGCCTT TAATGAACAT 
GTAGATGAGT GTCTTGATGG ACCGTCAACC AGTGAGAACT CAAAAATATC CTGTTACTCA 
CATGCTTCCT CTGCAGACAT TGGTCAGAAG GAAGATGTAC ACCCCTCTAT TCCACTGTGT 
GAGAAACGGG GGCATGAAAA TGGAGAGATC ACTTTAGTAG ATGGTGTAGA TCTAACAGGG 
ACGGAAGACA GATCATTGAA AGCAGCAAGG ATGGACACTC TAGAGAATAA TCGCAGCAAA 
GAGGAATGTC CTGATATTCC AGACAAGTCT TGTCCTATAT CACTGGAAAA TGAAACCATC 
AGTACATTAA GTAGGCAAGA CTCTGTCCAG CCTTGTACAG ATGAGGTAGT AACAGGACGA 
GCTCTAGTGT GTCCTGTTTG TAACCTAGAA CAAGAGACTT CTGATCTTAC CCTCTTCAAC 
ATACATGTGG ATATTTGCTT AAATAAAGGT ATTATCCAAG AACTGAGAAA TAGTGAAGGT 
AATTCAGTTA AACAACCCAA AGAAAGCTCG AGAAGTACTG ACAGACTTCA GAAGGCTTCA 
GGAAGGACCA AAAGGCCAGG AACGAAGACA AAGAGCTCAA CTTTGAAGAA AACAAAGCCC 
CGAGATCCCA GACACACCCT TGATGGATTT TTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polk ( XM_030247285.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103145.1 Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu49059
Clone ID Related Accession (Same CDS sequence)	NM_001347602.1 , XM_030247285.1
Accession Version	XM_030247285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA polymerase kappa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAACA CAAAGGAAAA GGACAACTTC AAAGACGACC TCCTGCTCCG CATGGGACTA 
AACGATAACA AAGCAGGCAT GGAAGGGTTG GATAAGGAGA AAATTAACAA AATTATCATG 
GAAGCCACAA AGGGGTCCAG ATTTTATGGA AATGAGCTCA AGAAGGAAAA GCAAGTCAAT 
CAACGGATTG AAAATATGAT GCAACAAAAA GCTCAAATTA CCAGCCAGCA ACTAAGGAAA 
GCTCAATTAC AGGTTGACAA ATTTGCAATG GAGTTAGAAC GGAACCGGAA TTTGAACAAT 
ACCATAGTTC ATGTTGACAT GGACGCTTTC TATGCAGCTG TGGAAATGAG GGACAACCCG 
GAACTGAAGG ATAAACCCAT TGCTGTAGGA TCCATGAGCA TGTTGGCTAC TTCGAATTAC 
CATGCAAGGA GGTTTGGTGT CCGTGCAGCC ATGCCAGGAT TTATTGCTAA GAGGCTCTGC 
CCACAACTTA TTATAGTGCC CCCAAACTTT GACAAATATA GAGCTGTGAG TAAGGAGGTT 
AAGGAGATAC TTGCTGAATA TGATCCCAAT TTTATGGCCA TGAGTCTGGA CGAAGCCTAC 
TTGAATATAA CACAGCACTT GCAGGAAAGG CAAGATTGGC CTGAGGACAA AAGAAGATAC 
TTCATCAAGA TGGGAAACTA CTTAAAAATC GCGATGATGA CATTGGATTT GGCTGCTAGT 
AACTTGAACC TACTGAGGCC AGCTAAACCT CCAGGCATCG CCCCCAATAC AATGTTAGCA 
AAAGTGTGCA GTGATAAGAA TAAGCCAAAC GGACAGTACC AGATCCTTCC CAGCAGGAGC 
GCGGTGATGG ACTTCATCAA GGACCTGCCT ATTAGAAAGG TTTCTGGGAT AGGAAAAGTT 
ACAGAGAAAA TGTTAATGGC TCTCGGGATT GTTACTTGCA CAGAACTCTA CCAACAGAGA 
GCGTTGCTGT CTCTCCTTTT CTCTGAAACC TCTTGGCATT ATTTTCTTCA CATCGCGCTG 
GGTCTAGGTT CAACAGACCT GGCAAGGGAT GGAGAAAGGA AAAGCATGAG TGTTGAAAGG 
ACATTCAGTG AGATAAGTAA GACAGAGGAA CAGTACAGCC TGTGCCAAGA ACTGTGCGCT 
GAGCTCGCCC ACGACCTCCA GAAGGAAGGA CTTAAGGGAA GAACCGTCAC CATTAAGCTG 
AAGAACGTGA ATTTTGAAGT AAAAACTCGT GCATCTACCG TTCCGGCCGC CATTTCTACT 
GCAGAGGAAA TATTTGCCAT TGCCAAGGAG CTGCTAAGGA CAGAAGTTAA TGTGGGTTCT 
CCACACCCCC TGCGGTTAAG ACTGATGGGT GTCCGAATGT CTACTTTTTC CAGTGAAGAT 
GACAGGAAAC ACCAACAAAG GAGCATCATT GGTTTCTTAC AAGCTGGAAA CCAAGCTTTG 
TCATCTACTG GGGATAGTCT AGACAAAACT GACAAAACTG AGCTTGCAAA GCCCTTAGAA 
ATGTCTCATA AGAAGAGTTT CTTTGATAAA AAGCGATCAG AAAGAATCTC CAACTGTCAA 
GACACATCCA GATGTAAAAC TGCGGGTCAG CAAGCTTTAC AGATCTTGGA ACCATCCCAA 
GCATTAAAGA AGCTGAGCGA GAGTTTTGAA ACATCAGAGA ATTCAAATGA CTGTCAGACA 
TTTATATGTC CAGTTTGCTT TAGGGAGCAA GAAGGTGTCA GTCTGGAAGC CTTTAATGAA 
CATGTAGATG AGTGTCTTGA TGGACCGTCA ACCAGTGAGA ACTCAAAAAT ATCCTGTTAC 
TCACATGCTT CCTCTGCAGA CATTGGTCAG AAGGAAGATG TACACCCCTC TATTCCACTG 
TGTGAGAAAC GGGGGCATGA AAATGGAGAG ATCACTTTAG TAGATGGTGT AGATCTAACA 
GGGACGGAAG ACAGATCATT GAAAGCAGCA AGGATGGACA CTCTAGAGAA TAATCGCAGC 
AAAGAGGAAT GTCCTGATAT TCCAGACAAG TCTTGTCCTA TATCACTGGA AAATGAAACC 
ATCAGTACAT TAAGTAGGCA AGACTCTGTC CAGCCTTGTA CAGATGAGGT AGTAACAGGA 
CGAGCTCTAG TGTGTCCTGT TTGTAACCTA GAACAAGAGA CTTCTGATCT TACCCTCTTC 
AACATACATG TGGATATTTG CTTAAATAAA GGTATTATCC AAGAACTGAG AAATAGTGAA 
GGTAATTCAG TTAAACAACC CAAAGAAAGC TCGAGAAGTA CTGACAGACT TCAGAAGGCT 
TCAGGAAGGA CCAAAAGGCC AGGAACGAAG ACAAAGAGCT CAACTTTGAA GAAAACAAAG 
CCCCGAGATC CCAGACACAC CCTTGATGGA TTTTTTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103145.1
CDS	214..2595
Translation	MDNTKEKDNFKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDKFAMELERNRNLNNTIVHVDMDAFYAAVEMRDNPELKDKPIAVGSMSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILAEYDPNFMAMSLDEAYLNITQHLQERQDWPEDKRRYFIKMGNYLKIAMMTLDLAASNLNLLRPAKPPGIAPNTMLAKVCSDKNKPNGQYQILPSRSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMSVERTFSEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLRTEVNVGSPHPLRLRLMGVRMSTFSSEDDRKHQQRSIIGFLQAGNQALSSTGDSLDKTDKTELAKPLEMSHKKSFFDKKRSERISNCQDTSRCKTAGQQALQILEPSQALKKLSESFETSENSNDCQTFICPVCFREQEGVSLEAFNEHVDECLDGPSTSENSKISCYSHASSADIGQKEDVHPSIPLCEKRGHENGEITLVDGVDLTGTEDRSLKAARMDTLENNRSKEECPDIPDKSCPISLENETISTLSRQDSVQPCTDEVVTGRALVCPVCNLEQETSDLTLFNIHVDICLNKGIIQELRNSEGNSVKQPKESSRSTDRLQKASGRTKRPGTKTKSSTLKKTKPRDPRHTLDGFFK

Target ORF information:

RefSeq Version	XM_030247285.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247285.1

ORF Insert Sequence:

ATGGATAACA CAAAGGAAAA GGACAACTTC AAAGACGACC TCCTGCTCCG CATGGGACTA 
AACGATAACA AAGCAGGCAT GGAAGGGTTG GATAAGGAGA AAATTAACAA AATTATCATG 
GAAGCCACAA AGGGGTCCAG ATTTTATGGA AATGAGCTCA AGAAGGAAAA GCAAGTCAAT 
CAACGGATTG AAAATATGAT GCAACAAAAA GCTCAAATTA CCAGCCAGCA ACTAAGGAAA 
GCTCAATTAC AGGTTGACAA ATTTGCAATG GAGTTAGAAC GGAACCGGAA TTTGAACAAT 
ACCATAGTTC ATGTTGACAT GGACGCTTTC TATGCAGCTG TGGAAATGAG GGACAACCCG 
GAACTGAAGG ATAAACCCAT TGCTGTAGGA TCCATGAGCA TGTTGGCTAC TTCGAATTAC 
CATGCAAGGA GGTTTGGTGT CCGTGCAGCC ATGCCAGGAT TTATTGCTAA GAGGCTCTGC 
CCACAACTTA TTATAGTGCC CCCAAACTTT GACAAATATA GAGCTGTGAG TAAGGAGGTT 
AAGGAGATAC TTGCTGAATA TGATCCCAAT TTTATGGCCA TGAGTCTGGA CGAAGCCTAC 
TTGAATATAA CACAGCACTT GCAGGAAAGG CAAGATTGGC CTGAGGACAA AAGAAGATAC 
TTCATCAAGA TGGGAAACTA CTTAAAAATC GCGATGATGA CATTGGATTT GGCTGCTAGT 
AACTTGAACC TACTGAGGCC AGCTAAACCT CCAGGCATCG CCCCCAATAC AATGTTAGCA 
AAAGTGTGCA GTGATAAGAA TAAGCCAAAC GGACAGTACC AGATCCTTCC CAGCAGGAGC 
GCGGTGATGG ACTTCATCAA GGACCTGCCT ATTAGAAAGG TTTCTGGGAT AGGAAAAGTT 
ACAGAGAAAA TGTTAATGGC TCTCGGGATT GTTACTTGCA CAGAACTCTA CCAACAGAGA 
GCGTTGCTGT CTCTCCTTTT CTCTGAAACC TCTTGGCATT ATTTTCTTCA CATCGCGCTG 
GGTCTAGGTT CAACAGACCT GGCAAGGGAT GGAGAAAGGA AAAGCATGAG TGTTGAAAGG 
ACATTCAGTG AGATAAGTAA GACAGAGGAA CAGTACAGCC TGTGCCAAGA ACTGTGCGCT 
GAGCTCGCCC ACGACCTCCA GAAGGAAGGA CTTAAGGGAA GAACCGTCAC CATTAAGCTG 
AAGAACGTGA ATTTTGAAGT AAAAACTCGT GCATCTACCG TTCCGGCCGC CATTTCTACT 
GCAGAGGAAA TATTTGCCAT TGCCAAGGAG CTGCTAAGGA CAGAAGTTAA TGTGGGTTCT 
CCACACCCCC TGCGGTTAAG ACTGATGGGT GTCCGAATGT CTACTTTTTC CAGTGAAGAT 
GACAGGAAAC ACCAACAAAG GAGCATCATT GGTTTCTTAC AAGCTGGAAA CCAAGCTTTG 
TCATCTACTG GGGATAGTCT AGACAAAACT GACAAAACTG AGCTTGCAAA GCCCTTAGAA 
ATGTCTCATA AGAAGAGTTT CTTTGATAAA AAGCGATCAG AAAGAATCTC CAACTGTCAA 
GACACATCCA GATGTAAAAC TGCGGGTCAG CAAGCTTTAC AGATCTTGGA ACCATCCCAA 
GCATTAAAGA AGCTGAGCGA GAGTTTTGAA ACATCAGAGA ATTCAAATGA CTGTCAGACA 
TTTATATGTC CAGTTTGCTT TAGGGAGCAA GAAGGTGTCA GTCTGGAAGC CTTTAATGAA 
CATGTAGATG AGTGTCTTGA TGGACCGTCA ACCAGTGAGA ACTCAAAAAT ATCCTGTTAC 
TCACATGCTT CCTCTGCAGA CATTGGTCAG AAGGAAGATG TACACCCCTC TATTCCACTG 
TGTGAGAAAC GGGGGCATGA AAATGGAGAG ATCACTTTAG TAGATGGTGT AGATCTAACA 
GGGACGGAAG ACAGATCATT GAAAGCAGCA AGGATGGACA CTCTAGAGAA TAATCGCAGC 
AAAGAGGAAT GTCCTGATAT TCCAGACAAG TCTTGTCCTA TATCACTGGA AAATGAAACC 
ATCAGTACAT TAAGTAGGCA AGACTCTGTC CAGCCTTGTA CAGATGAGGT AGTAACAGGA 
CGAGCTCTAG TGTGTCCTGT TTGTAACCTA GAACAAGAGA CTTCTGATCT TACCCTCTTC 
AACATACATG TGGATATTTG CTTAAATAAA GGTATTATCC AAGAACTGAG AAATAGTGAA 
GGTAATTCAG TTAAACAACC CAAAGAAAGC TCGAGAAGTA CTGACAGACT TCAGAAGGCT 
TCAGGAAGGA CCAAAAGGCC AGGAACGAAG ACAAAGAGCT CAACTTTGAA GAAAACAAAG 
CCCCGAGATC CCAGACACAC CCTTGATGGA TTTTTTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polk ( XM_006517680.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517743.1 Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant X2, mRNA.

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CloneID	OMu49060
Clone ID Related Accession (Same CDS sequence)	XM_006517680.4 , XM_006517680.3
Accession Version	XM_006517680.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA polymerase kappa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517680.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAC AAAAAGCTCA AATTACCAGC CAGCAACTAA GGAAAGCTCA ATTACAGGTT 
GACAAATTTG CAATGGAGTT AGAACGGAAC CGGAATTTGA ACAATACCAT AGTTCATGTT 
GACATGGACG CTTTCTATGC AGCTGTGGAA ATGAGGGACA ACCCGGAACT GAAGGATAAA 
CCCATTGCTG TAGGATCCAT GAGCATGTTG GCTACTTCGA ATTACCATGC AAGGAGGTTT 
GGTGTCCGTG CAGCCATGCC AGGATTTATT GCTAAGAGGC TCTGCCCACA ACTTATTATA 
GTGCCCCCAA ACTTTGACAA ATATAGAGCT GTGAGTAAGG AGGTTAAGGA GATACTTGCT 
GAATATGATC CCAATTTTAT GGCCATGAGT CTGGACGAAG CCTACTTGAA TATAACACAG 
CACTTGCAGG AAAGGCAAGA TTGGCCTGAG GACAAAAGAA GATACTTCAT CAAGATGGGA 
AACTACTTAA AAATCGACAC ACCCAGACAG GAAGCTAACG AGCTGACTGA GTATGAGCGG 
TCCATCTCCC CGCTGCTTTT TGAAGATAGT CCTCCTGATT TGCAACCCCA AGGAAGTCCT 
TTCCAACTGA ACTCTGAAGA ACAAAACAAT CCTCAAATAG CCCAAAATTC AGTTGTTTTT 
GGAACATCAG CTGAGGAAGT GGTAAAGGAA ATTCGCTTCA GAATTGAACA AAAAACAACG 
CTGACAGCCA GCGCAGGCAT CGCCCCCAAT ACAATGTTAG CAAAAGTGTG CAGTGATAAG 
AATAAGCCAA ACGGACAGTA CCAGATCCTT CCCAGCAGGA GCGCGGTGAT GGACTTCATC 
AAGGACCTGC CTATTAGAAA GGTTTCTGGG ATAGGAAAAG TTACAGAGAA AATGTTAATG 
GCTCTCGGGA TTGTTACTTG CACAGAACTC TACCAACAGA GAGCGTTGCT GTCTCTCCTT 
TTCTCTGAAA CCTCTTGGCA TTATTTTCTT CACATCGCGC TGGGTCTAGG TTCAACAGAC 
CTGGCAAGGG ATGGAGAAAG GAAAAGCATG AGTGTTGAAA GGACATTCAG TGAGATAAGT 
AAGACAGAGG AACAGTACAG CCTGTGCCAA GAACTGTGCG CTGAGCTCGC CCACGACCTC 
CAGAAGGAAG GACTTAAGGG AAGAACCGTC ACCATTAAGC TGAAGAACGT GAATTTTGAA 
GTAAAAACTC GTGCATCTAC CGTTCCGGCC GCCATTTCTA CTGCAGAGGA AATATTTGCC 
ATTGCCAAGG AGCTGCTAAG GACAGAAGTT AATGTGGGTT CTCCACACCC CCTGCGGTTA 
AGACTGATGG GTGTCCGAAT GTCTACTTTT TCCAGTGAAG ATGACAGGAA ACACCAACAA 
AGGAGCATCA TTGGTTTCTT ACAAGCTGGA AACCAAGCTT TGTCATCTAC TGGGGATAGT 
CTAGACAAAA CTGACAAAAC TGAGCTTGCA AAGCCCTTAG AAATGTCTCA TAAGAAGAGT 
TTCTTTGATA AAAAGCGATC AGAAAGAATC TCCAACTGTC AAGACACATC CAGATGTAAA 
ACTGCGGGTC AGCAAGCTTT ACAGATCTTG GAACCATCCC AAGCATTAAA GAAGCTGAGC 
GAGAGTTTTG AAACATCAGA GAATTCAAAT GACTGTCAGA CATTTATATG TCCAGTTTGC 
TTTAGGGAGC AAGAAGGTGT CAGTCTGGAA GCCTTTAATG AACATGTAGA TGAGTGTCTT 
GATGGACCGT CAACCAGTGA GAACTCAAAA ATATCCTGTT ACTCACATGC TTCCTCTGCA 
GACATTGGTC AGAAGGAAGA TGTACACCCC TCTATTCCAC TGTGTGAGAA ACGGGGGCAT 
GAAAATGGAG AGATCACTTT AGTAGATGGT GTAGATCTAA CAGGGACGGA AGACAGATCA 
TTGAAAGCAG CAAGGATGGA CACTCTAGAG AATAATCGCA GCAAAGAGGA ATGTCCTGAT 
ATTCCAGACA AGTCTTGTCC TATATCACTG GAAAATGAAA CCATCAGTAC ATTAAGTAGG 
CAAGACTCTG TCCAGCCTTG TACAGATGAG GTAGTAACAG GACGAGCTCT AGTGTGTCCT 
GTTTGTAACC TAGAACAAGA GACTTCTGAT CTTACCCTCT TCAACATACA TGTGGATATT 
TGCTTAAATA AAGGTATTAT CCAAGAACTG AGAAATAGTG AAGGTAATTC AGTTAAACAA 
CCCAAAGAAA GCTCGAGAAG TACTGACAGA CTTCAGAAGG CTTCAGGAAG GACCAAAAGG 
CCAGGAACGA AGACAAAGAG CTCAACTTTG AAGAAAACAA AGCCCCGAGA TCCCAGACAC 
ACCCTTGATG GATTTTTTAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517743.1
CDS	148..2511
Translation	MMQQKAQITSQQLRKAQLQVDKFAMELERNRNLNNTIVHVDMDAFYAAVEMRDNPELKDKPIAVGSMSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILAEYDPNFMAMSLDEAYLNITQHLQERQDWPEDKRRYFIKMGNYLKIDTPRQEANELTEYERSISPLLFEDSPPDLQPQGSPFQLNSEEQNNPQIAQNSVVFGTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSRSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMSVERTFSEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLRTEVNVGSPHPLRLRLMGVRMSTFSSEDDRKHQQRSIIGFLQAGNQALSSTGDSLDKTDKTELAKPLEMSHKKSFFDKKRSERISNCQDTSRCKTAGQQALQILEPSQALKKLSESFETSENSNDCQTFICPVCFREQEGVSLEAFNEHVDECLDGPSTSENSKISCYSHASSADIGQKEDVHPSIPLCEKRGHENGEITLVDGVDLTGTEDRSLKAARMDTLENNRSKEECPDIPDKSCPISLENETISTLSRQDSVQPCTDEVVTGRALVCPVCNLEQETSDLTLFNIHVDICLNKGIIQELRNSEGNSVKQPKESSRSTDRLQKASGRTKRPGTKTKSSTLKKTKPRDPRHTLDGFFK

Target ORF information:

RefSeq Version	XM_006517680.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517680.4

ORF Insert Sequence:

ATGATGCAAC AAAAAGCTCA AATTACCAGC CAGCAACTAA GGAAAGCTCA ATTACAGGTT 
GACAAATTTG CAATGGAGTT AGAACGGAAC CGGAATTTGA ACAATACCAT AGTTCATGTT 
GACATGGACG CTTTCTATGC AGCTGTGGAA ATGAGGGACA ACCCGGAACT GAAGGATAAA 
CCCATTGCTG TAGGATCCAT GAGCATGTTG GCTACTTCGA ATTACCATGC AAGGAGGTTT 
GGTGTCCGTG CAGCCATGCC AGGATTTATT GCTAAGAGGC TCTGCCCACA ACTTATTATA 
GTGCCCCCAA ACTTTGACAA ATATAGAGCT GTGAGTAAGG AGGTTAAGGA GATACTTGCT 
GAATATGATC CCAATTTTAT GGCCATGAGT CTGGACGAAG CCTACTTGAA TATAACACAG 
CACTTGCAGG AAAGGCAAGA TTGGCCTGAG GACAAAAGAA GATACTTCAT CAAGATGGGA 
AACTACTTAA AAATCGACAC ACCCAGACAG GAAGCTAACG AGCTGACTGA GTATGAGCGG 
TCCATCTCCC CGCTGCTTTT TGAAGATAGT CCTCCTGATT TGCAACCCCA AGGAAGTCCT 
TTCCAACTGA ACTCTGAAGA ACAAAACAAT CCTCAAATAG CCCAAAATTC AGTTGTTTTT 
GGAACATCAG CTGAGGAAGT GGTAAAGGAA ATTCGCTTCA GAATTGAACA AAAAACAACG 
CTGACAGCCA GCGCAGGCAT CGCCCCCAAT ACAATGTTAG CAAAAGTGTG CAGTGATAAG 
AATAAGCCAA ACGGACAGTA CCAGATCCTT CCCAGCAGGA GCGCGGTGAT GGACTTCATC 
AAGGACCTGC CTATTAGAAA GGTTTCTGGG ATAGGAAAAG TTACAGAGAA AATGTTAATG 
GCTCTCGGGA TTGTTACTTG CACAGAACTC TACCAACAGA GAGCGTTGCT GTCTCTCCTT 
TTCTCTGAAA CCTCTTGGCA TTATTTTCTT CACATCGCGC TGGGTCTAGG TTCAACAGAC 
CTGGCAAGGG ATGGAGAAAG GAAAAGCATG AGTGTTGAAA GGACATTCAG TGAGATAAGT 
AAGACAGAGG AACAGTACAG CCTGTGCCAA GAACTGTGCG CTGAGCTCGC CCACGACCTC 
CAGAAGGAAG GACTTAAGGG AAGAACCGTC ACCATTAAGC TGAAGAACGT GAATTTTGAA 
GTAAAAACTC GTGCATCTAC CGTTCCGGCC GCCATTTCTA CTGCAGAGGA AATATTTGCC 
ATTGCCAAGG AGCTGCTAAG GACAGAAGTT AATGTGGGTT CTCCACACCC CCTGCGGTTA 
AGACTGATGG GTGTCCGAAT GTCTACTTTT TCCAGTGAAG ATGACAGGAA ACACCAACAA 
AGGAGCATCA TTGGTTTCTT ACAAGCTGGA AACCAAGCTT TGTCATCTAC TGGGGATAGT 
CTAGACAAAA CTGACAAAAC TGAGCTTGCA AAGCCCTTAG AAATGTCTCA TAAGAAGAGT 
TTCTTTGATA AAAAGCGATC AGAAAGAATC TCCAACTGTC AAGACACATC CAGATGTAAA 
ACTGCGGGTC AGCAAGCTTT ACAGATCTTG GAACCATCCC AAGCATTAAA GAAGCTGAGC 
GAGAGTTTTG AAACATCAGA GAATTCAAAT GACTGTCAGA CATTTATATG TCCAGTTTGC 
TTTAGGGAGC AAGAAGGTGT CAGTCTGGAA GCCTTTAATG AACATGTAGA TGAGTGTCTT 
GATGGACCGT CAACCAGTGA GAACTCAAAA ATATCCTGTT ACTCACATGC TTCCTCTGCA 
GACATTGGTC AGAAGGAAGA TGTACACCCC TCTATTCCAC TGTGTGAGAA ACGGGGGCAT 
GAAAATGGAG AGATCACTTT AGTAGATGGT GTAGATCTAA CAGGGACGGA AGACAGATCA 
TTGAAAGCAG CAAGGATGGA CACTCTAGAG AATAATCGCA GCAAAGAGGA ATGTCCTGAT 
ATTCCAGACA AGTCTTGTCC TATATCACTG GAAAATGAAA CCATCAGTAC ATTAAGTAGG 
CAAGACTCTG TCCAGCCTTG TACAGATGAG GTAGTAACAG GACGAGCTCT AGTGTGTCCT 
GTTTGTAACC TAGAACAAGA GACTTCTGAT CTTACCCTCT TCAACATACA TGTGGATATT 
TGCTTAAATA AAGGTATTAT CCAAGAACTG AGAAATAGTG AAGGTAATTC AGTTAAACAA 
CCCAAAGAAA GCTCGAGAAG TACTGACAGA CTTCAGAAGG CTTCAGGAAG GACCAAAAGG 
CCAGGAACGA AGACAAAGAG CTCAACTTTG AAGAAAACAA AGCCCCGAGA TCCCAGACAC 
ACCCTTGATG GATTTTTTAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Limited ability of DNA polymerase kappa to suppress benzo[a]pyrene-induced genotoxicity in vivo.
Environmental and molecular mutagenesis58(9)644-653(2017 12)
Masumura K,Toyoda-Hokaiwado N,Niimi N,Gr?z P,Wada NA,Takeiri A,Jishage KI,Mishima M,Nohmi T

Effects of the N terminus of mouse DNA polymerase ? on the bypass of a guanine-benzo[a]pyrenyl adduct.
Journal of biochemistry159(4)471-9(2016 Apr)
Liu Y,Ma X,Guo C

Interferon regulatory factor 8 (IRF8) interacts with the B cell lymphoma 6 (BCL6) corepressor BCOR.
The Journal of biological chemistry289(49)34250-7(2014 Dec)
Yoon J,Feng X,Kim YS,Shin DM,Hatzi K,Wang H,Morse HC 3rd

In vivo evidence that DNA polymerase kappa is responsible for error-free bypass across DNA cross-links induced by mitomycin C.
DNA repair24113-121(2014 Dec)
Takeiri A,Wada NA,Motoyama S,Matsuzaki K,Tateishi H,Matsumoto K,Niimi N,Sassa A,Gr?z P,Masumura K,Yamada M,Mishima M,Jishage KI,Nohmi T

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Polk cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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