Plch1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plch1
Entrez Gene ID	269437
Full Name	phospholipase C, eta 1
Synonyms	BC042549,PLC-eta-1,PLCeta1,Plcl3
General protein information	Preferred Names phospholipase C, eta 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 phosphoinositide phospholipase C-eta-1 phospholipase C-like protein 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 E1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006501535.3	XP_006501598.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
XM_006501536.3	XP_006501599.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
XM_006501541.3	XP_006501604.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X9
NM_001177732.1	NP_001171203.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 3
NM_001177733.2	NP_001171204.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 2
XM_011240131.3	XP_011238433.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_006501533.4	XP_006501596.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_006501536.4	XP_006501599.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
XM_030252632.1	XP_030108492.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
XM_017319595.2	XP_017175084.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
XM_017319598.2	XP_017175087.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X11
XM_017319599.2	XP_017175088.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X12
XM_006501537.3	XP_006501600.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
NM_183191.3	NP_899014.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 1
XM_006501530.3	XP_006501593.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_006501531.3	XP_006501594.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_006501538.4	XP_006501601.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
XM_006501541.4	XP_006501604.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X9
XM_017319593.1	XP_017175082.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_017319598.1	XP_017175087.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X11
XM_006501530.4	XP_006501593.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_006501531.4	XP_006501594.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_011240134.2	XP_011238436.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
XM_017319601.2	XP_017175090.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X14
XM_017319597.1	XP_017175086.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X10
XM_006501533.3	XP_006501596.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_006501538.3	XP_006501601.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
XM_017319596.1	XP_017175085.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X8
XM_017319600.1	XP_017175089.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X13
XM_017319601.1	XP_017175090.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X14
NM_001177733.1	NP_001171204.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 2
XM_017319593.2	XP_017175082.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_017319594.1	XP_017175083.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_006501535.4	XP_006501598.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
XM_011240133.2	XP_011238435.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
XM_017319596.2	XP_017175085.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X8
XM_030252633.1	XP_030108493.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X10
XM_017319600.2	XP_017175089.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X13
NM_001370984.1	NP_001357913.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 4
NM_183191.4	NP_899014.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 1
XM_011240131.2	XP_011238433.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_017319595.1	XP_017175084.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
XM_017319599.1	XP_017175088.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X12
NM_001177732.2	NP_001171203.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 3
NM_001370985.1	NP_001357914.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PLCH1	XP_001149022.1
	LOC707243	XP_002808381.1
Bos taurus (cattle)	PLCH1	XP_002685035.3
Rattus norvegicus (Norway rat)	Plch1	NP_001178636.1
Xenopus tropicalis (tropical clawed frog)	plch1	XP_002933304.2
Homo sapiens (human)	PLCH1	NP_001124432.1
Gallus gallus (chicken)	PLCH1	XP_004943620.1
Danio rerio (zebrafish)	LOC566479	XP_694841.5
Mus musculus (house mouse)	Plch1	NP_899014.2
Canis lupus familiaris (dog)	PLCH1	XP_005634651.1

Gene Ontology
Bibliography

Related articles in PubMed

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Phospholipase C? hydrolyzes perinuclear phosphatidylinositol 4-phosphate to regulate cardiac hypertrophy.
Zhang L, Malik S, Pang J, Wang H, Park KM, Yule DI, Blaxall BC, Smrcka AV
Cell153(1)216-27(2013 Mar)

Phospholipase C-?1 is activated by intracellular Ca(2+) mobilization and enhances GPCRs/PLC/Ca(2+) signaling.
Kim JK, Choi JW, Lim S, Kwon O, Seo JK, Ryu SH, Suh PG
Cellular signalling23(6)1022-9(2011 Jun)

Epac2-dependent mobilization of intracellular Ca?+ by glucagon-like peptide-1 receptor agonist exendin-4 is disrupted in ?-cells of phospholipase C-? knockout mice.
Dzhura I, Chepurny OG, Kelley GG, Leech CA, Roe MW, Dzhura E, Afshari P, Malik S, Rindler MJ, Xu X, Lu Y, Smrcka AV, Holz GG
The Journal of physiology588(Pt 24)4871-89(2010 Dec)

Molecular cloning and characterization of a novel phospholipase C, PLC-eta.
Hwang JI, Oh YS, Shin KJ, Kim H, Ryu SH, Suh PG
The Biochemical journal389(Pt 1)181-6(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study concludes that perinuclear PLCepsilon, scaffolded to mAKAP in cardiac myocytes, responds to hypertrophic stimuli to generate diacylglycerol (DAG) from PI4P in the Golgi apparatus, in close proximity to the nuclear envelope, to regulate activation of nuclear PKD and hypertrophic signaling pathways.
Title: Phospholipase C? hydrolyzes perinuclear phosphatidylinositol 4-phosphate to regulate cardiac hypertrophy.

PLC-eta1 is activated via intracellular calcium mobilization from the endoplasmic reticulum, and therefore amplifies G-protein coupled receptor mediated signaling
Title: Phospholipase C-?1 is activated by intracellular Ca(2+) mobilization and enhances GPCRs/PLC/Ca(2+) signaling.

Phospholipase C-eta was found to be expressed in mouse beta-cells, and knockout of that gene disrupts the action of exendin-4 to facilitate calcium-induced calcium release in the beta-cells of these mice.
Title: Epac2-dependent mobilization of intracellular Ca?+ by glucagon-like peptide-1 receptor agonist exendin-4 is disrupted in ?-cells of phospholipase C-? knockout mice.

The identification of a novel PLC isoform in the brains of human and mouse, named PLC-eta, which contains the conserved pleckstrin homology domain, X and Y domains for catalytic activity and the C2 domain.
Title: Molecular cloning and characterization of a novel phospholipase C, PLC-eta.

molecular cloning of PLC-eta1 and PLC-eta2.
Title: Identification of a novel class of mammalian phosphoinositol-specific phospholipase C enzymes.

The following Plch1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plch1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu80632	XM_017319601.2 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu80628	NM_001370985.1 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu31955	XM_006501541.4 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu80627	XM_017319596.2 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu80628	XM_017319597.1 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu80629	XM_017319598.2 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu80630	XM_017319599.2 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu80631	XM_017319600.2 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu88233	XM_030252633.1 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu15560	NM_001177732.2 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$587.30 $839.00
OMu15578	NM_001177733.2 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu31948	XM_006501533.4 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu31948	XM_017319593.2 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu31948	XM_011240131.3 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu31948	XM_006501530.4 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu31948	XM_006501531.4 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu31949	XM_006501535.4 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu31950	XM_006501536.4 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu31952	XM_006501538.4 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu80626	XM_017319595.2 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu88232	XM_030252632.1 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu15698	NM_183191.4 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu31951	NM_001370984.1 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu31948	XM_017319594.1 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu31951	XM_006501537.3 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu31954	XM_011240134.2 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu31954	XM_011240133.2 Latest version!	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu31950	XM_006501536.3	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu31955	XM_006501541.3	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu80629	XM_017319598.1	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu31948	XM_006501530.3	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu31948	XM_006501531.3	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu31948	XM_006501533.3	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu31952	XM_006501538.3	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu80632	XM_017319601.1	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu15578	NM_001177733.1	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu31948	XM_017319593.1	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu31949	XM_006501535.3	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu80627	XM_017319596.1	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu80631	XM_017319600.1	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu15698	NM_183191.3	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu31948	XM_011240131.2	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu80626	XM_017319595.1	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu80630	XM_017319599.1	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu15560	NM_001177732.1	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$587.30 $839.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plch1 ( XM_017319601.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175090.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OMu80632
Clone ID Related Accession (Same CDS sequence)	XM_017319601.2 , XM_017319601.1
Accession Version	XM_017319601.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319601.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGTAC AGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175090.1
CDS	404..3391
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLVQI

Target ORF information:

RefSeq Version	XM_017319601.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319601.2

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGTAC AGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319601.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175090.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X20, mRNA.

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CloneID	OMu80632
Clone ID Related Accession (Same CDS sequence)	XM_017319601.2 , XM_017319601.1
Accession Version	XM_017319601.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGTAC AGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175090.1
CDS	624..3611
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLVQI

Target ORF information:

RefSeq Version	XM_017319601.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319601.1

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGTAC AGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( NM_001370985.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001357914.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 5, mRNA.

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CloneID	OMu80628
Clone ID Related Accession (Same CDS sequence)	XM_017319597.1 , NM_001370985.1
Accession Version	NM_001370985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-19
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116732.10 and AC121285.7. On Jun 20, 2019 this sequence version replaced XM_017319597.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.237788.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGAA AGCTACGAAA 
TCACGTTCTA AATCCTATAG TACTGATGAT GAGGATGATA GCCTTCAGAA TCCTGGCAAG 
GAGGGAGGCC AGCTGTACAG GCTGGGCCGC CGAAGGAGAA CCATGAAACT CTGCAGAGAG 
CTCTCGGACT TGGTGGTGTA CACAAACTCC GTGGCAGCCC AGGATATTGT TGATGATGGA 
ACCACAGGGA ATGTGTTGTC CTTCAGCGAA ACAAGAGCCC ATCAAGTGGT TCAGCAGAAG 
TCAGAGCAGT TCATGATCTA CAACCAAAAA CAGCTCACAA GGATTTACCC CTCTGCCTAC 
CGCATCGATT CCAGTAACTT CAACCCACTG CCCTACTGGA ATGCAGGCTG CCAGCTCGTG 
GCTCTGAACT ACCAGTCTGA AGGGCGAATG ATGCAGATCA ATCGAGCAAA ATTCAAGGCG 
AATGGCAACT GTGGCTATAT TCTCAAGCCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCACTTCC TGCCAACCCC AAAAAACAGC TCATACTGAA AGTGATCAGT 
GGACAGCAAC TCCCCAAACC TCCTGACTCT ATGTTTGGAG ACCGAGGCGA GATCATTGAC 
CCTTTCGTTG AAGTTGAAAT TATTGGGCTG CCAGTAGACT GTTGCAAGGA CCAAACTCGT 
GTGGTGGATG ACAACGGATT TAACCCTGTG TGGGAAGAAA CCCTAACTTT TACAGTTCAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ATGATCCTAT TGGAAGAGAC 
TTTGTCGGAC AAAGGACTGT GACCTTCAGT AGCCTCGTGC CCGGCTACCG GCATGTCTAC 
CTGGAAGGAC TCACAGAAGC ATCTATTTTC GTTCACATAA CCATCAATGA GATCTTTGGA 
AAGTGGAGTC CTTTAATCCT CAACCCCAGT TATACCATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AGCTCCAAGG CCTGAAGGGG CTGTTCAATA AGAATCCCAG GCACGCTTCT 
TCAGAAAACA ACTCGCATTA TGTTCGGAAG CGATCGATTG GAGATAGGAT TCTGCGACGT 
ACAGCTAGTG CTCCAGCCAA AGGCAGGAAA AAGAGCAAAG TGGGCTTCCA AGAAATGGTT 
GAGATAAAAG ATTCTGTCTC TGAGGCTTCA AGAGATCAAG ATGGTGTCCT GAGGAGGACC 
ACCCGGAGTC TGCAAGTACG CCCTGTCTCT ATGCCTGTTG ACAAGAGCCT TCTGGGGGCT 
TTGTCGCTGC CCATATCTGA AGCAGCAAAA GACACGGATG GAAAAGAAAA CTGTCTGGAT 
CTGAATAGGA AGCAGCGCAA GCAGGAGACA AGGATGACAG AAGAAAGGGA GCCGCAACTA 
GAAAAGACCC ACATTTTTCA AATTTCAACA AAAAGTTATC CTCCTCCTCC AGTGCGCTCC 
TCCACAAAGA TGCCAACCAA GGGCCAACTG CCAGTGTATC AAACCCAGAA CAGTGTGGAG 
GACGAGGTGC AAAGAGTGAG AGGATCAAAC CAAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357914.1
CDS	295..3513
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLDLNRKQRKQETRMTEEREPQLEKTHIFQISTKSYPPPPVRSSTKMPTKGQLPVYQTQNSVEDEVQRVRGSNQI

Target ORF information:

RefSeq Version	NM_001370985.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370985.1

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGAA AGCTACGAAA 
TCACGTTCTA AATCCTATAG TACTGATGAT GAGGATGATA GCCTTCAGAA TCCTGGCAAG 
GAGGGAGGCC AGCTGTACAG GCTGGGCCGC CGAAGGAGAA CCATGAAACT CTGCAGAGAG 
CTCTCGGACT TGGTGGTGTA CACAAACTCC GTGGCAGCCC AGGATATTGT TGATGATGGA 
ACCACAGGGA ATGTGTTGTC CTTCAGCGAA ACAAGAGCCC ATCAAGTGGT TCAGCAGAAG 
TCAGAGCAGT TCATGATCTA CAACCAAAAA CAGCTCACAA GGATTTACCC CTCTGCCTAC 
CGCATCGATT CCAGTAACTT CAACCCACTG CCCTACTGGA ATGCAGGCTG CCAGCTCGTG 
GCTCTGAACT ACCAGTCTGA AGGGCGAATG ATGCAGATCA ATCGAGCAAA ATTCAAGGCG 
AATGGCAACT GTGGCTATAT TCTCAAGCCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCACTTCC TGCCAACCCC AAAAAACAGC TCATACTGAA AGTGATCAGT 
GGACAGCAAC TCCCCAAACC TCCTGACTCT ATGTTTGGAG ACCGAGGCGA GATCATTGAC 
CCTTTCGTTG AAGTTGAAAT TATTGGGCTG CCAGTAGACT GTTGCAAGGA CCAAACTCGT 
GTGGTGGATG ACAACGGATT TAACCCTGTG TGGGAAGAAA CCCTAACTTT TACAGTTCAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ATGATCCTAT TGGAAGAGAC 
TTTGTCGGAC AAAGGACTGT GACCTTCAGT AGCCTCGTGC CCGGCTACCG GCATGTCTAC 
CTGGAAGGAC TCACAGAAGC ATCTATTTTC GTTCACATAA CCATCAATGA GATCTTTGGA 
AAGTGGAGTC CTTTAATCCT CAACCCCAGT TATACCATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AGCTCCAAGG CCTGAAGGGG CTGTTCAATA AGAATCCCAG GCACGCTTCT 
TCAGAAAACA ACTCGCATTA TGTTCGGAAG CGATCGATTG GAGATAGGAT TCTGCGACGT 
ACAGCTAGTG CTCCAGCCAA AGGCAGGAAA AAGAGCAAAG TGGGCTTCCA AGAAATGGTT 
GAGATAAAAG ATTCTGTCTC TGAGGCTTCA AGAGATCAAG ATGGTGTCCT GAGGAGGACC 
ACCCGGAGTC TGCAAGTACG CCCTGTCTCT ATGCCTGTTG ACAAGAGCCT TCTGGGGGCT 
TTGTCGCTGC CCATATCTGA AGCAGCAAAA GACACGGATG GAAAAGAAAA CTGTCTGGAT 
CTGAATAGGA AGCAGCGCAA GCAGGAGACA AGGATGACAG AAGAAAGGGA GCCGCAACTA 
GAAAAGACCC ACATTTTTCA AATTTCAACA AAAAGTTATC CTCCTCCTCC AGTGCGCTCC 
TCCACAAAGA TGCCAACCAA GGGCCAACTG CCAGTGTATC AAACCCAGAA CAGTGTGGAG 
GACGAGGTGC AAAGAGTGAG AGGATCAAAC CAAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501541.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501604.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X15, mRNA.

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CloneID	OMu31955
Clone ID Related Accession (Same CDS sequence)	XM_006501541.3 , XM_006501541.4
Accession Version	XM_006501541.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501541.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGATC TGAATAGGAA GCAGCGCAAG 
GAGACAAGGA TGACAGAAGA AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT 
TCAACAAAAA GTTATCCTCC TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC 
CAACTGCCAG TGTATCAAAC CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA 
TCAAACCAAA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501604.1
CDS	404..3658
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLDLNRKQRKETRMTEEREPQLEKTHIFQISTKSYPPPPVRSSTKMPTKGQLPVYQTQNSVEDEVQRVRGSNQI

Target ORF information:

RefSeq Version	XM_006501541.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501541.4

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGATC TGAATAGGAA GCAGCGCAAG 
GAGACAAGGA TGACAGAAGA AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT 
TCAACAAAAA GTTATCCTCC TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC 
CAACTGCCAG TGTATCAAAC CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA 
TCAAACCAAA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319596.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175085.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu80627
Clone ID Related Accession (Same CDS sequence)	XM_017319596.1 , XM_017319596.2
Accession Version	XM_017319596.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGATC TGAATAGGAA GCAGCGCAAG 
CAGGAGACAA GGATGACAGA AGAAAGGGAG CCGCAACTAG AAAAGACCCA CATTTTTCAA 
ATTTCAACAA AAAGTTATCC TCCTCCTCCA GTGCGCTCCT CCACAAAGAT GCCAACCAAG 
GGCCAACTGC CAGTGTATCA AACCCAGAAC AGTGTGGAGG ACGAGGTGCA AAGAGTGAGA 
GGATCAAACC AAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175085.1
CDS	626..3883
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLDLNRKQRKQETRMTEEREPQLEKTHIFQISTKSYPPPPVRSSTKMPTKGQLPVYQTQNSVEDEVQRVRGSNQI

Target ORF information:

RefSeq Version	XM_017319596.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319596.1

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGATC TGAATAGGAA GCAGCGCAAG 
CAGGAGACAA GGATGACAGA AGAAAGGGAG CCGCAACTAG AAAAGACCCA CATTTTTCAA 
ATTTCAACAA AAAGTTATCC TCCTCCTCCA GTGCGCTCCT CCACAAAGAT GCCAACCAAG 
GGCCAACTGC CAGTGTATCA AACCCAGAAC AGTGTGGAGG ACGAGGTGCA AAGAGTGAGA 
GGATCAAACC AAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319596.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175085.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu80627
Clone ID Related Accession (Same CDS sequence)	XM_017319596.1 , XM_017319596.2
Accession Version	XM_017319596.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319596.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGATC TGAATAGGAA GCAGCGCAAG 
CAGGAGACAA GGATGACAGA AGAAAGGGAG CCGCAACTAG AAAAGACCCA CATTTTTCAA 
ATTTCAACAA AAAGTTATCC TCCTCCTCCA GTGCGCTCCT CCACAAAGAT GCCAACCAAG 
GGCCAACTGC CAGTGTATCA AACCCAGAAC AGTGTGGAGG ACGAGGTGCA AAGAGTGAGA 
GGATCAAACC AAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175085.1
CDS	404..3661
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLDLNRKQRKQETRMTEEREPQLEKTHIFQISTKSYPPPPVRSSTKMPTKGQLPVYQTQNSVEDEVQRVRGSNQI

Target ORF information:

RefSeq Version	XM_017319596.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319596.2

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGATC TGAATAGGAA GCAGCGCAAG 
CAGGAGACAA GGATGACAGA AGAAAGGGAG CCGCAACTAG AAAAGACCCA CATTTTTCAA 
ATTTCAACAA AAAGTTATCC TCCTCCTCCA GTGCGCTCCT CCACAAAGAT GCCAACCAAG 
GGCCAACTGC CAGTGTATCA AACCCAGAAC AGTGTGGAGG ACGAGGTGCA AAGAGTGAGA 
GGATCAAACC AAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319597.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175086.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu80628
Clone ID Related Accession (Same CDS sequence)	XM_017319597.1 , NM_001370985.1
Accession Version	XM_017319597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGAA AGCTACGAAA 
TCACGTTCTA AATCCTATAG TACTGATGAT GAGGATGATA GCCTTCAGAA TCCTGGCAAG 
GAGGGAGGCC AGCTGTACAG GCTGGGCCGC CGAAGGAGAA CCATGAAACT CTGCAGAGAG 
CTCTCGGACT TGGTGGTGTA CACAAACTCC GTGGCAGCCC AGGATATTGT TGATGATGGA 
ACCACAGGGA ATGTGTTGTC CTTCAGCGAA ACAAGAGCCC ATCAAGTGGT TCAGCAGAAG 
TCAGAGCAGT TCATGATCTA CAACCAAAAA CAGCTCACAA GGATTTACCC CTCTGCCTAC 
CGCATCGATT CCAGTAACTT CAACCCACTG CCCTACTGGA ATGCAGGCTG CCAGCTCGTG 
GCTCTGAACT ACCAGTCTGA AGGGCGAATG ATGCAGATCA ATCGAGCAAA ATTCAAGGCG 
AATGGCAACT GTGGCTATAT TCTCAAGCCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCACTTCC TGCCAACCCC AAAAAACAGC TCATACTGAA AGTGATCAGT 
GGACAGCAAC TCCCCAAACC TCCTGACTCT ATGTTTGGAG ACCGAGGCGA GATCATTGAC 
CCTTTCGTTG AAGTTGAAAT TATTGGGCTG CCAGTAGACT GTTGCAAGGA CCAAACTCGT 
GTGGTGGATG ACAACGGATT TAACCCTGTG TGGGAAGAAA CCCTAACTTT TACAGTTCAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ATGATCCTAT TGGAAGAGAC 
TTTGTCGGAC AAAGGACTGT GACCTTCAGT AGCCTCGTGC CCGGCTACCG GCATGTCTAC 
CTGGAAGGAC TCACAGAAGC ATCTATTTTC GTTCACATAA CCATCAATGA GATCTTTGGA 
AAGTGGAGTC CTTTAATCCT CAACCCCAGT TATACCATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AGCTCCAAGG CCTGAAGGGG CTGTTCAATA AGAATCCCAG GCACGCTTCT 
TCAGAAAACA ACTCGCATTA TGTTCGGAAG CGATCGATTG GAGATAGGAT TCTGCGACGT 
ACAGCTAGTG CTCCAGCCAA AGGCAGGAAA AAGAGCAAAG TGGGCTTCCA AGAAATGGTT 
GAGATAAAAG ATTCTGTCTC TGAGGCTTCA AGAGATCAAG ATGGTGTCCT GAGGAGGACC 
ACCCGGAGTC TGCAAGTACG CCCTGTCTCT ATGCCTGTTG ACAAGAGCCT TCTGGGGGCT 
TTGTCGCTGC CCATATCTGA AGCAGCAAAA GACACGGATG GAAAAGAAAA CTGTCTGGAT 
CTGAATAGGA AGCAGCGCAA GCAGGAGACA AGGATGACAG AAGAAAGGGA GCCGCAACTA 
GAAAAGACCC ACATTTTTCA AATTTCAACA AAAAGTTATC CTCCTCCTCC AGTGCGCTCC 
TCCACAAAGA TGCCAACCAA GGGCCAACTG CCAGTGTATC AAACCCAGAA CAGTGTGGAG 
GACGAGGTGC AAAGAGTGAG AGGATCAAAC CAAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175086.1
CDS	888..4106
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLDLNRKQRKQETRMTEEREPQLEKTHIFQISTKSYPPPPVRSSTKMPTKGQLPVYQTQNSVEDEVQRVRGSNQI

Target ORF information:

RefSeq Version	XM_017319597.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319597.1

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGAA AGCTACGAAA 
TCACGTTCTA AATCCTATAG TACTGATGAT GAGGATGATA GCCTTCAGAA TCCTGGCAAG 
GAGGGAGGCC AGCTGTACAG GCTGGGCCGC CGAAGGAGAA CCATGAAACT CTGCAGAGAG 
CTCTCGGACT TGGTGGTGTA CACAAACTCC GTGGCAGCCC AGGATATTGT TGATGATGGA 
ACCACAGGGA ATGTGTTGTC CTTCAGCGAA ACAAGAGCCC ATCAAGTGGT TCAGCAGAAG 
TCAGAGCAGT TCATGATCTA CAACCAAAAA CAGCTCACAA GGATTTACCC CTCTGCCTAC 
CGCATCGATT CCAGTAACTT CAACCCACTG CCCTACTGGA ATGCAGGCTG CCAGCTCGTG 
GCTCTGAACT ACCAGTCTGA AGGGCGAATG ATGCAGATCA ATCGAGCAAA ATTCAAGGCG 
AATGGCAACT GTGGCTATAT TCTCAAGCCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCACTTCC TGCCAACCCC AAAAAACAGC TCATACTGAA AGTGATCAGT 
GGACAGCAAC TCCCCAAACC TCCTGACTCT ATGTTTGGAG ACCGAGGCGA GATCATTGAC 
CCTTTCGTTG AAGTTGAAAT TATTGGGCTG CCAGTAGACT GTTGCAAGGA CCAAACTCGT 
GTGGTGGATG ACAACGGATT TAACCCTGTG TGGGAAGAAA CCCTAACTTT TACAGTTCAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ATGATCCTAT TGGAAGAGAC 
TTTGTCGGAC AAAGGACTGT GACCTTCAGT AGCCTCGTGC CCGGCTACCG GCATGTCTAC 
CTGGAAGGAC TCACAGAAGC ATCTATTTTC GTTCACATAA CCATCAATGA GATCTTTGGA 
AAGTGGAGTC CTTTAATCCT CAACCCCAGT TATACCATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AGCTCCAAGG CCTGAAGGGG CTGTTCAATA AGAATCCCAG GCACGCTTCT 
TCAGAAAACA ACTCGCATTA TGTTCGGAAG CGATCGATTG GAGATAGGAT TCTGCGACGT 
ACAGCTAGTG CTCCAGCCAA AGGCAGGAAA AAGAGCAAAG TGGGCTTCCA AGAAATGGTT 
GAGATAAAAG ATTCTGTCTC TGAGGCTTCA AGAGATCAAG ATGGTGTCCT GAGGAGGACC 
ACCCGGAGTC TGCAAGTACG CCCTGTCTCT ATGCCTGTTG ACAAGAGCCT TCTGGGGGCT 
TTGTCGCTGC CCATATCTGA AGCAGCAAAA GACACGGATG GAAAAGAAAA CTGTCTGGAT 
CTGAATAGGA AGCAGCGCAA GCAGGAGACA AGGATGACAG AAGAAAGGGA GCCGCAACTA 
GAAAAGACCC ACATTTTTCA AATTTCAACA AAAAGTTATC CTCCTCCTCC AGTGCGCTCC 
TCCACAAAGA TGCCAACCAA GGGCCAACTG CCAGTGTATC AAACCCAGAA CAGTGTGGAG 
GACGAGGTGC AAAGAGTGAG AGGATCAAAC CAAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319598.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175087.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OMu80629
Clone ID Related Accession (Same CDS sequence)	XM_017319598.2 , XM_017319598.1
Accession Version	XM_017319598.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319598.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGATC TGAATAGGAA GCAGCGCAAG 
CAGGAGACAA GGATGACAGA AGAAAGGGAG CCGCAACTAG AAAAGACCCA CATTTTTCAA 
ATTTCAACAA AAAGTTATCC TCCTCCTCCA GTGCGCTCCT CCACAAAGAT GCCAACCAAG 
GGCCAACTGC CAGTGTATCA AACCCAGAAC AGTGTGGAGG ACGAGGTGCA AAGAGTGAGA 
GGATCAAACC AAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175087.1
CDS	404..3601
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLDLNRKQRKQETRMTEEREPQLEKTHIFQISTKSYPPPPVRSSTKMPTKGQLPVYQTQNSVEDEVQRVRGSNQI

Target ORF information:

RefSeq Version	XM_017319598.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319598.2

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGATC TGAATAGGAA GCAGCGCAAG 
CAGGAGACAA GGATGACAGA AGAAAGGGAG CCGCAACTAG AAAAGACCCA CATTTTTCAA 
ATTTCAACAA AAAGTTATCC TCCTCCTCCA GTGCGCTCCT CCACAAAGAT GCCAACCAAG 
GGCCAACTGC CAGTGTATCA AACCCAGAAC AGTGTGGAGG ACGAGGTGCA AAGAGTGAGA 
GGATCAAACC AAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319598.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175087.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OMu80629
Clone ID Related Accession (Same CDS sequence)	XM_017319598.2 , XM_017319598.1
Accession Version	XM_017319598.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGATC TGAATAGGAA GCAGCGCAAG 
CAGGAGACAA GGATGACAGA AGAAAGGGAG CCGCAACTAG AAAAGACCCA CATTTTTCAA 
ATTTCAACAA AAAGTTATCC TCCTCCTCCA GTGCGCTCCT CCACAAAGAT GCCAACCAAG 
GGCCAACTGC CAGTGTATCA AACCCAGAAC AGTGTGGAGG ACGAGGTGCA AAGAGTGAGA 
GGATCAAACC AAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175087.1
CDS	624..3821
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLDLNRKQRKQETRMTEEREPQLEKTHIFQISTKSYPPPPVRSSTKMPTKGQLPVYQTQNSVEDEVQRVRGSNQI

Target ORF information:

RefSeq Version	XM_017319598.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319598.1

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGATC TGAATAGGAA GCAGCGCAAG 
CAGGAGACAA GGATGACAGA AGAAAGGGAG CCGCAACTAG AAAAGACCCA CATTTTTCAA 
ATTTCAACAA AAAGTTATCC TCCTCCTCCA GTGCGCTCCT CCACAAAGAT GCCAACCAAG 
GGCCAACTGC CAGTGTATCA AACCCAGAAC AGTGTGGAGG ACGAGGTGCA AAGAGTGAGA 
GGATCAAACC AAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319599.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175088.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OMu80630
Clone ID Related Accession (Same CDS sequence)	XM_017319599.2 , XM_017319599.1
Accession Version	XM_017319599.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319599.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGTAC AGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175088.1
CDS	404..3451
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLVQI

Target ORF information:

RefSeq Version	XM_017319599.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319599.2

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGTAC AGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319599.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175088.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OMu80630
Clone ID Related Accession (Same CDS sequence)	XM_017319599.2 , XM_017319599.1
Accession Version	XM_017319599.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGTAC AGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175088.1
CDS	625..3672
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLVQI

Target ORF information:

RefSeq Version	XM_017319599.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319599.1

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGTAC AGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319600.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175089.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OMu80631
Clone ID Related Accession (Same CDS sequence)	XM_017319600.1 , XM_017319600.2
Accession Version	XM_017319600.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGT GGAGTCCTTT AATCCTCAAC 
CCCAGTTATA CCATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGTACAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175089.1
CDS	626..3670
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLVQI

Target ORF information:

RefSeq Version	XM_017319600.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319600.1

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGT GGAGTCCTTT AATCCTCAAC 
CCCAGTTATA CCATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGTACAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319600.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175089.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X19, mRNA.

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CloneID	OMu80631
Clone ID Related Accession (Same CDS sequence)	XM_017319600.1 , XM_017319600.2
Accession Version	XM_017319600.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319600.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGT GGAGTCCTTT AATCCTCAAC 
CCCAGTTATA CCATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGTACAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175089.1
CDS	404..3448
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLVQI

Target ORF information:

RefSeq Version	XM_017319600.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319600.2

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGT GGAGTCCTTT AATCCTCAAC 
CCCAGTTATA CCATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGTACAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_030252633.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108493.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X16, mRNA.

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CloneID	OMu88233
Clone ID Related Accession (Same CDS sequence)	XM_030252633.1
Accession Version	XM_030252633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGT GGAGTCCTTT AATCCTCAAC 
CCCAGTTATA CCATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGATCTGA ATAGGAAGCA GCGCAAGCAG 
GAGACAAGGA TGACAGAAGA AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT 
TCAACAAAAA GTTATCCTCC TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC 
CAACTGCCAG TGTATCAAAC CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA 
TCAAACCAAA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108493.1
CDS	404..3658
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLDLNRKQRKQETRMTEEREPQLEKTHIFQISTKSYPPPPVRSSTKMPTKGQLPVYQTQNSVEDEVQRVRGSNQI

Target ORF information:

RefSeq Version	XM_030252633.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252633.1

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGT GGAGTCCTTT AATCCTCAAC 
CCCAGTTATA CCATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGATCTGA ATAGGAAGCA GCGCAAGCAG 
GAGACAAGGA TGACAGAAGA AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT 
TCAACAAAAA GTTATCCTCC TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC 
CAACTGCCAG TGTATCAAAC CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA 
TCAAACCAAA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( NM_001177732.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171203.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu15560
Clone ID Related Accession (Same CDS sequence)	NM_001177732.1 , NM_001177732.2
Accession Version	NM_001177732.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY691172.1 and BQ177171.1. Transcript Variant: This variant (3, also known as PLC-eta1a) differs in the 3' UTR and includes an alternate exon that causes a frameshift in the 3' coding region, compared to variant 1. This results in an isoform (3, also known as phospholipase C-eta1a) that has a shorter and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AY691172.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGCA GAAAGCTACG 
AAATCACGTT CTAAATCCTA TAGTACTGAT GATGAGGATG ATAGCCTTCA GAATCCTGGC 
AAGGAGGGAG GCCAGCTGTA CAGGCTGGGC CGCCGAAGGA GAACCATGAA ACTCTGCAGA 
GAGCTCTCGG ACTTGGTGGT GTACACAAAC TCCGTGGCAG CCCAGGATAT TGTTGATGAT 
GGAACCACAG GGAATGTGTT GTCCTTCAGC GAAACAAGAG CCCATCAAGT GGTTCAGCAG 
AAGTCAGAGC AGTTCATGAT CTACAACCAA AAACAGCTCA CAAGGATTTA CCCCTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCA CTGCCCTACT GGAATGCAGG CTGCCAGCTC 
GTGGCTCTGA ACTACCAGTC TGAAGGGCGA ATGATGCAGA TCAATCGAGC AAAATTCAAG 
GCGAATGGCA ACTGTGGCTA TATTCTCAAG CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTTCTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAAC AGCTCATACT GAAAGTGATC 
AGTGGACAGC AACTCCCCAA ACCTCCTGAC TCTATGTTTG GAGACCGAGG CGAGATCATT 
GACCCTTTCG TTGAAGTTGA AATTATTGGG CTGCCAGTAG ACTGTTGCAA GGACCAAACT 
CGTGTGGTGG ATGACAACGG ATTTAACCCT GTGTGGGAAG AAACCCTAAC TTTTACAGTT 
CACATGCCAG AAATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC TATTGGAAGA 
GACTTTGTCG GACAAAGGAC TGTGACCTTC AGTAGCCTCG TGCCCGGCTA CCGGCATGTC 
TACCTGGAAG GACTCACAGA AGCATCTATT TTCGTTCACA TAACCATCAA TGAGATCTTT 
GGAAAGTGGA GTCCTTTAAT CCTCAACCCC AGTTATACCA TATTGCACTT TCTAGGAGCT 
ACAAAGAACA GACAGCTCCA AGGCCTGAAG GGGCTGTTCA ATAAGAATCC CAGGCACGCT 
TCTTCAGAAA ACAACTCGCA TTATGTTCGG AAGCGATCGA TTGGAGATAG GATTCTGCGA 
CGTACAGCTA GTGCTCCAGC CAAAGGCAGG AAAAAGAGCA AAGTGGGCTT CCAAGAAATG 
GTTGAGATAA AAGATTCTGT CTCTGAGGCT TCAAGAGATC AAGATGGTGT CCTGAGGAGG 
ACCACCCGGA GTCTGCAAGT ACGCCCTGTC TCTATGCCTG TTGACAAGAG CCTTCTGGGG 
GCTTTGTCGC TGCCCATATC TGAAGCAGCA AAAGACACGG ATGGAAAAGA AAACTGTCTG 
GTACAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171203.1
CDS	1..3012
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLVQI

Target ORF information:

RefSeq Version	NM_001177732.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177732.1

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGCA GAAAGCTACG 
AAATCACGTT CTAAATCCTA TAGTACTGAT GATGAGGATG ATAGCCTTCA GAATCCTGGC 
AAGGAGGGAG GCCAGCTGTA CAGGCTGGGC CGCCGAAGGA GAACCATGAA ACTCTGCAGA 
GAGCTCTCGG ACTTGGTGGT GTACACAAAC TCCGTGGCAG CCCAGGATAT TGTTGATGAT 
GGAACCACAG GGAATGTGTT GTCCTTCAGC GAAACAAGAG CCCATCAAGT GGTTCAGCAG 
AAGTCAGAGC AGTTCATGAT CTACAACCAA AAACAGCTCA CAAGGATTTA CCCCTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCA CTGCCCTACT GGAATGCAGG CTGCCAGCTC 
GTGGCTCTGA ACTACCAGTC TGAAGGGCGA ATGATGCAGA TCAATCGAGC AAAATTCAAG 
GCGAATGGCA ACTGTGGCTA TATTCTCAAG CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTTCTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAAC AGCTCATACT GAAAGTGATC 
AGTGGACAGC AACTCCCCAA ACCTCCTGAC TCTATGTTTG GAGACCGAGG CGAGATCATT 
GACCCTTTCG TTGAAGTTGA AATTATTGGG CTGCCAGTAG ACTGTTGCAA GGACCAAACT 
CGTGTGGTGG ATGACAACGG ATTTAACCCT GTGTGGGAAG AAACCCTAAC TTTTACAGTT 
CACATGCCAG AAATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC TATTGGAAGA 
GACTTTGTCG GACAAAGGAC TGTGACCTTC AGTAGCCTCG TGCCCGGCTA CCGGCATGTC 
TACCTGGAAG GACTCACAGA AGCATCTATT TTCGTTCACA TAACCATCAA TGAGATCTTT 
GGAAAGTGGA GTCCTTTAAT CCTCAACCCC AGTTATACCA TATTGCACTT TCTAGGAGCT 
ACAAAGAACA GACAGCTCCA AGGCCTGAAG GGGCTGTTCA ATAAGAATCC CAGGCACGCT 
TCTTCAGAAA ACAACTCGCA TTATGTTCGG AAGCGATCGA TTGGAGATAG GATTCTGCGA 
CGTACAGCTA GTGCTCCAGC CAAAGGCAGG AAAAAGAGCA AAGTGGGCTT CCAAGAAATG 
GTTGAGATAA AAGATTCTGT CTCTGAGGCT TCAAGAGATC AAGATGGTGT CCTGAGGAGG 
ACCACCCGGA GTCTGCAAGT ACGCCCTGTC TCTATGCCTG TTGACAAGAG CCTTCTGGGG 
GCTTTGTCGC TGCCCATATC TGAAGCAGCA AAAGACACGG ATGGAAAAGA AAACTGTCTG 
GTACAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( NM_001177732.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171203.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu15560
Clone ID Related Accession (Same CDS sequence)	NM_001177732.1 , NM_001177732.2
Accession Version	NM_001177732.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-19
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116732.10 and AC121285.7. On Jun 20, 2019 this sequence version replaced NM_001177732.1. Transcript Variant: This variant (3, also known as PLC-eta1a) differs in the 3' UTR and includes an alternate exon that causes a frameshift in the 3' coding region, compared to variant 1. This results in an isoform (3, also known as phospholipase C-eta1a) that has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AY691172.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGCA GAAAGCTACG 
AAATCACGTT CTAAATCCTA TAGTACTGAT GATGAGGATG ATAGCCTTCA GAATCCTGGC 
AAGGAGGGAG GCCAGCTGTA CAGGCTGGGC CGCCGAAGGA GAACCATGAA ACTCTGCAGA 
GAGCTCTCGG ACTTGGTGGT GTACACAAAC TCCGTGGCAG CCCAGGATAT TGTTGATGAT 
GGAACCACAG GGAATGTGTT GTCCTTCAGC GAAACAAGAG CCCATCAAGT GGTTCAGCAG 
AAGTCAGAGC AGTTCATGAT CTACAACCAA AAACAGCTCA CAAGGATTTA CCCCTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCA CTGCCCTACT GGAATGCAGG CTGCCAGCTC 
GTGGCTCTGA ACTACCAGTC TGAAGGGCGA ATGATGCAGA TCAATCGAGC AAAATTCAAG 
GCGAATGGCA ACTGTGGCTA TATTCTCAAG CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTTCTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAAC AGCTCATACT GAAAGTGATC 
AGTGGACAGC AACTCCCCAA ACCTCCTGAC TCTATGTTTG GAGACCGAGG CGAGATCATT 
GACCCTTTCG TTGAAGTTGA AATTATTGGG CTGCCAGTAG ACTGTTGCAA GGACCAAACT 
CGTGTGGTGG ATGACAACGG ATTTAACCCT GTGTGGGAAG AAACCCTAAC TTTTACAGTT 
CACATGCCAG AAATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC TATTGGAAGA 
GACTTTGTCG GACAAAGGAC TGTGACCTTC AGTAGCCTCG TGCCCGGCTA CCGGCATGTC 
TACCTGGAAG GACTCACAGA AGCATCTATT TTCGTTCACA TAACCATCAA TGAGATCTTT 
GGAAAGTGGA GTCCTTTAAT CCTCAACCCC AGTTATACCA TATTGCACTT TCTAGGAGCT 
ACAAAGAACA GACAGCTCCA AGGCCTGAAG GGGCTGTTCA ATAAGAATCC CAGGCACGCT 
TCTTCAGAAA ACAACTCGCA TTATGTTCGG AAGCGATCGA TTGGAGATAG GATTCTGCGA 
CGTACAGCTA GTGCTCCAGC CAAAGGCAGG AAAAAGAGCA AAGTGGGCTT CCAAGAAATG 
GTTGAGATAA AAGATTCTGT CTCTGAGGCT TCAAGAGATC AAGATGGTGT CCTGAGGAGG 
ACCACCCGGA GTCTGCAAGT ACGCCCTGTC TCTATGCCTG TTGACAAGAG CCTTCTGGGG 
GCTTTGTCGC TGCCCATATC TGAAGCAGCA AAAGACACGG ATGGAAAAGA AAACTGTCTG 
GTACAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171203.1
CDS	295..3306
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLVQI

Target ORF information:

RefSeq Version	NM_001177732.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177732.2

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGCA GAAAGCTACG 
AAATCACGTT CTAAATCCTA TAGTACTGAT GATGAGGATG ATAGCCTTCA GAATCCTGGC 
AAGGAGGGAG GCCAGCTGTA CAGGCTGGGC CGCCGAAGGA GAACCATGAA ACTCTGCAGA 
GAGCTCTCGG ACTTGGTGGT GTACACAAAC TCCGTGGCAG CCCAGGATAT TGTTGATGAT 
GGAACCACAG GGAATGTGTT GTCCTTCAGC GAAACAAGAG CCCATCAAGT GGTTCAGCAG 
AAGTCAGAGC AGTTCATGAT CTACAACCAA AAACAGCTCA CAAGGATTTA CCCCTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCA CTGCCCTACT GGAATGCAGG CTGCCAGCTC 
GTGGCTCTGA ACTACCAGTC TGAAGGGCGA ATGATGCAGA TCAATCGAGC AAAATTCAAG 
GCGAATGGCA ACTGTGGCTA TATTCTCAAG CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTTCTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAAC AGCTCATACT GAAAGTGATC 
AGTGGACAGC AACTCCCCAA ACCTCCTGAC TCTATGTTTG GAGACCGAGG CGAGATCATT 
GACCCTTTCG TTGAAGTTGA AATTATTGGG CTGCCAGTAG ACTGTTGCAA GGACCAAACT 
CGTGTGGTGG ATGACAACGG ATTTAACCCT GTGTGGGAAG AAACCCTAAC TTTTACAGTT 
CACATGCCAG AAATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC TATTGGAAGA 
GACTTTGTCG GACAAAGGAC TGTGACCTTC AGTAGCCTCG TGCCCGGCTA CCGGCATGTC 
TACCTGGAAG GACTCACAGA AGCATCTATT TTCGTTCACA TAACCATCAA TGAGATCTTT 
GGAAAGTGGA GTCCTTTAAT CCTCAACCCC AGTTATACCA TATTGCACTT TCTAGGAGCT 
ACAAAGAACA GACAGCTCCA AGGCCTGAAG GGGCTGTTCA ATAAGAATCC CAGGCACGCT 
TCTTCAGAAA ACAACTCGCA TTATGTTCGG AAGCGATCGA TTGGAGATAG GATTCTGCGA 
CGTACAGCTA GTGCTCCAGC CAAAGGCAGG AAAAAGAGCA AAGTGGGCTT CCAAGAAATG 
GTTGAGATAA AAGATTCTGT CTCTGAGGCT TCAAGAGATC AAGATGGTGT CCTGAGGAGG 
ACCACCCGGA GTCTGCAAGT ACGCCCTGTC TCTATGCCTG TTGACAAGAG CCTTCTGGGG 
GCTTTGTCGC TGCCCATATC TGAAGCAGCA AAAGACACGG ATGGAAAAGA AAACTGTCTG 
GTACAGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( NM_001177733.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171204.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15578
Clone ID Related Accession (Same CDS sequence)	NM_001177733.2 , NM_001177733.1
Accession Version	NM_001177733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-19
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116732.10 and AC121285.7. On Jun 20, 2019 this sequence version replaced NM_001177733.1. Transcript Variant: This variant (2, also known as PLC-eta1b) includes an alternate exon that causes a frameshift in the 3' coding region, compared to variant 1. This results in an isoform (2, also known as phospholipase C-eta1b) that has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AY691173.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGCA GAAAGCTACG 
AAATCACGTT CTAAATCCTA TAGTACTGAT GATGAGGATG ATAGCCTTCA GAATCCTGGC 
AAGGAGGGAG GCCAGCTGTA CAGGCTGGGC CGCCGAAGGA GAACCATGAA ACTCTGCAGA 
GAGCTCTCGG ACTTGGTGGT GTACACAAAC TCCGTGGCAG CCCAGGATAT TGTTGATGAT 
GGAACCACAG GGAATGTGTT GTCCTTCAGC GAAACAAGAG CCCATCAAGT GGTTCAGCAG 
AAGTCAGAGC AGTTCATGAT CTACAACCAA AAACAGCTCA CAAGGATTTA CCCCTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCA CTGCCCTACT GGAATGCAGG CTGCCAGCTC 
GTGGCTCTGA ACTACCAGTC TGAAGGGCGA ATGATGCAGA TCAATCGAGC AAAATTCAAG 
GCGAATGGCA ACTGTGGCTA TATTCTCAAG CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTTCTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAAC AGCTCATACT GAAAGTGATC 
AGTGGACAGC AACTCCCCAA ACCTCCTGAC TCTATGTTTG GAGACCGAGG CGAGATCATT 
GACCCTTTCG TTGAAGTTGA AATTATTGGG CTGCCAGTAG ACTGTTGCAA GGACCAAACT 
CGTGTGGTGG ATGACAACGG ATTTAACCCT GTGTGGGAAG AAACCCTAAC TTTTACAGTT 
CACATGCCAG AAATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC TATTGGAAGA 
GACTTTGTCG GACAAAGGAC TGTGACCTTC AGTAGCCTCG TGCCCGGCTA CCGGCATGTC 
TACCTGGAAG GACTCACAGA AGCATCTATT TTCGTTCACA TAACCATCAA TGAGATCTTT 
GGAAAGTGGA GTCCTTTAAT CCTCAACCCC AGTTATACCA TATTGCACTT TCTAGGAGCT 
ACAAAGAACA GACAGCTCCA AGGCCTGAAG GGGCTGTTCA ATAAGAATCC CAGGCACGCT 
TCTTCAGAAA ACAACTCGCA TTATGTTCGG AAGCGATCGA TTGGAGATAG GATTCTGCGA 
CGTACAGCTA GTGCTCCAGC CAAAGGCAGG AAAAAGAGCA AAGTGGGCTT CCAAGAAATG 
GTTGAGATAA AAGATTCTGT CTCTGAGGCT TCAAGAGATC AAGATGGTGT CCTGAGGAGG 
ACCACCCGGA GTCTGCAAGT ACGCCCTGTC TCTATGCCTG TTGACAAGAG CCTTCTGGGG 
GCTTTGTCGC TGCCCATATC TGAAGCAGCA AAAGACACGG ATGGAAAAGA AAACTGTCTG 
GATCTGAATA GGAAGCAGCG CAAGCAGGAG ACAAGGATGA CAGAAGAAAG GGAGCCGCAA 
CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC TCCAGTGCGC 
TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA GAACAGTGTG 
GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171204.1
CDS	295..3516
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLDLNRKQRKQETRMTEEREPQLEKTHIFQISTKSYPPPPVRSSTKMPTKGQLPVYQTQNSVEDEVQRVRGSNQI

Target ORF information:

RefSeq Version	NM_001177733.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177733.2

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGCA GAAAGCTACG 
AAATCACGTT CTAAATCCTA TAGTACTGAT GATGAGGATG ATAGCCTTCA GAATCCTGGC 
AAGGAGGGAG GCCAGCTGTA CAGGCTGGGC CGCCGAAGGA GAACCATGAA ACTCTGCAGA 
GAGCTCTCGG ACTTGGTGGT GTACACAAAC TCCGTGGCAG CCCAGGATAT TGTTGATGAT 
GGAACCACAG GGAATGTGTT GTCCTTCAGC GAAACAAGAG CCCATCAAGT GGTTCAGCAG 
AAGTCAGAGC AGTTCATGAT CTACAACCAA AAACAGCTCA CAAGGATTTA CCCCTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCA CTGCCCTACT GGAATGCAGG CTGCCAGCTC 
GTGGCTCTGA ACTACCAGTC TGAAGGGCGA ATGATGCAGA TCAATCGAGC AAAATTCAAG 
GCGAATGGCA ACTGTGGCTA TATTCTCAAG CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTTCTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAAC AGCTCATACT GAAAGTGATC 
AGTGGACAGC AACTCCCCAA ACCTCCTGAC TCTATGTTTG GAGACCGAGG CGAGATCATT 
GACCCTTTCG TTGAAGTTGA AATTATTGGG CTGCCAGTAG ACTGTTGCAA GGACCAAACT 
CGTGTGGTGG ATGACAACGG ATTTAACCCT GTGTGGGAAG AAACCCTAAC TTTTACAGTT 
CACATGCCAG AAATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC TATTGGAAGA 
GACTTTGTCG GACAAAGGAC TGTGACCTTC AGTAGCCTCG TGCCCGGCTA CCGGCATGTC 
TACCTGGAAG GACTCACAGA AGCATCTATT TTCGTTCACA TAACCATCAA TGAGATCTTT 
GGAAAGTGGA GTCCTTTAAT CCTCAACCCC AGTTATACCA TATTGCACTT TCTAGGAGCT 
ACAAAGAACA GACAGCTCCA AGGCCTGAAG GGGCTGTTCA ATAAGAATCC CAGGCACGCT 
TCTTCAGAAA ACAACTCGCA TTATGTTCGG AAGCGATCGA TTGGAGATAG GATTCTGCGA 
CGTACAGCTA GTGCTCCAGC CAAAGGCAGG AAAAAGAGCA AAGTGGGCTT CCAAGAAATG 
GTTGAGATAA AAGATTCTGT CTCTGAGGCT TCAAGAGATC AAGATGGTGT CCTGAGGAGG 
ACCACCCGGA GTCTGCAAGT ACGCCCTGTC TCTATGCCTG TTGACAAGAG CCTTCTGGGG 
GCTTTGTCGC TGCCCATATC TGAAGCAGCA AAAGACACGG ATGGAAAAGA AAACTGTCTG 
GATCTGAATA GGAAGCAGCG CAAGCAGGAG ACAAGGATGA CAGAAGAAAG GGAGCCGCAA 
CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC TCCAGTGCGC 
TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA GAACAGTGTG 
GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( NM_001177733.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171204.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 2, mRNA.

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CloneID	OMu15578
Clone ID Related Accession (Same CDS sequence)	NM_001177733.2 , NM_001177733.1
Accession Version	NM_001177733.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY691173.1 and BQ177171.1. Transcript Variant: This variant (2, also known as PLC-eta1b) includes an alternate exon that causes a frameshift in the 3' coding region, compared to variant 1. This results in an isoform (2, also known as phospholipase C-eta1b) that has a shorter and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AY691173.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGCA GAAAGCTACG 
AAATCACGTT CTAAATCCTA TAGTACTGAT GATGAGGATG ATAGCCTTCA GAATCCTGGC 
AAGGAGGGAG GCCAGCTGTA CAGGCTGGGC CGCCGAAGGA GAACCATGAA ACTCTGCAGA 
GAGCTCTCGG ACTTGGTGGT GTACACAAAC TCCGTGGCAG CCCAGGATAT TGTTGATGAT 
GGAACCACAG GGAATGTGTT GTCCTTCAGC GAAACAAGAG CCCATCAAGT GGTTCAGCAG 
AAGTCAGAGC AGTTCATGAT CTACAACCAA AAACAGCTCA CAAGGATTTA CCCCTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCA CTGCCCTACT GGAATGCAGG CTGCCAGCTC 
GTGGCTCTGA ACTACCAGTC TGAAGGGCGA ATGATGCAGA TCAATCGAGC AAAATTCAAG 
GCGAATGGCA ACTGTGGCTA TATTCTCAAG CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTTCTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAAC AGCTCATACT GAAAGTGATC 
AGTGGACAGC AACTCCCCAA ACCTCCTGAC TCTATGTTTG GAGACCGAGG CGAGATCATT 
GACCCTTTCG TTGAAGTTGA AATTATTGGG CTGCCAGTAG ACTGTTGCAA GGACCAAACT 
CGTGTGGTGG ATGACAACGG ATTTAACCCT GTGTGGGAAG AAACCCTAAC TTTTACAGTT 
CACATGCCAG AAATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC TATTGGAAGA 
GACTTTGTCG GACAAAGGAC TGTGACCTTC AGTAGCCTCG TGCCCGGCTA CCGGCATGTC 
TACCTGGAAG GACTCACAGA AGCATCTATT TTCGTTCACA TAACCATCAA TGAGATCTTT 
GGAAAGTGGA GTCCTTTAAT CCTCAACCCC AGTTATACCA TATTGCACTT TCTAGGAGCT 
ACAAAGAACA GACAGCTCCA AGGCCTGAAG GGGCTGTTCA ATAAGAATCC CAGGCACGCT 
TCTTCAGAAA ACAACTCGCA TTATGTTCGG AAGCGATCGA TTGGAGATAG GATTCTGCGA 
CGTACAGCTA GTGCTCCAGC CAAAGGCAGG AAAAAGAGCA AAGTGGGCTT CCAAGAAATG 
GTTGAGATAA AAGATTCTGT CTCTGAGGCT TCAAGAGATC AAGATGGTGT CCTGAGGAGG 
ACCACCCGGA GTCTGCAAGT ACGCCCTGTC TCTATGCCTG TTGACAAGAG CCTTCTGGGG 
GCTTTGTCGC TGCCCATATC TGAAGCAGCA AAAGACACGG ATGGAAAAGA AAACTGTCTG 
GATCTGAATA GGAAGCAGCG CAAGCAGGAG ACAAGGATGA CAGAAGAAAG GGAGCCGCAA 
CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC TCCAGTGCGC 
TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA GAACAGTGTG 
GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171204.1
CDS	1..3222
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLDLNRKQRKQETRMTEEREPQLEKTHIFQISTKSYPPPPVRSSTKMPTKGQLPVYQTQNSVEDEVQRVRGSNQI

Target ORF information:

RefSeq Version	NM_001177733.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177733.1

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGCA GAAAGCTACG 
AAATCACGTT CTAAATCCTA TAGTACTGAT GATGAGGATG ATAGCCTTCA GAATCCTGGC 
AAGGAGGGAG GCCAGCTGTA CAGGCTGGGC CGCCGAAGGA GAACCATGAA ACTCTGCAGA 
GAGCTCTCGG ACTTGGTGGT GTACACAAAC TCCGTGGCAG CCCAGGATAT TGTTGATGAT 
GGAACCACAG GGAATGTGTT GTCCTTCAGC GAAACAAGAG CCCATCAAGT GGTTCAGCAG 
AAGTCAGAGC AGTTCATGAT CTACAACCAA AAACAGCTCA CAAGGATTTA CCCCTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCA CTGCCCTACT GGAATGCAGG CTGCCAGCTC 
GTGGCTCTGA ACTACCAGTC TGAAGGGCGA ATGATGCAGA TCAATCGAGC AAAATTCAAG 
GCGAATGGCA ACTGTGGCTA TATTCTCAAG CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTTCTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAAC AGCTCATACT GAAAGTGATC 
AGTGGACAGC AACTCCCCAA ACCTCCTGAC TCTATGTTTG GAGACCGAGG CGAGATCATT 
GACCCTTTCG TTGAAGTTGA AATTATTGGG CTGCCAGTAG ACTGTTGCAA GGACCAAACT 
CGTGTGGTGG ATGACAACGG ATTTAACCCT GTGTGGGAAG AAACCCTAAC TTTTACAGTT 
CACATGCCAG AAATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC TATTGGAAGA 
GACTTTGTCG GACAAAGGAC TGTGACCTTC AGTAGCCTCG TGCCCGGCTA CCGGCATGTC 
TACCTGGAAG GACTCACAGA AGCATCTATT TTCGTTCACA TAACCATCAA TGAGATCTTT 
GGAAAGTGGA GTCCTTTAAT CCTCAACCCC AGTTATACCA TATTGCACTT TCTAGGAGCT 
ACAAAGAACA GACAGCTCCA AGGCCTGAAG GGGCTGTTCA ATAAGAATCC CAGGCACGCT 
TCTTCAGAAA ACAACTCGCA TTATGTTCGG AAGCGATCGA TTGGAGATAG GATTCTGCGA 
CGTACAGCTA GTGCTCCAGC CAAAGGCAGG AAAAAGAGCA AAGTGGGCTT CCAAGAAATG 
GTTGAGATAA AAGATTCTGT CTCTGAGGCT TCAAGAGATC AAGATGGTGT CCTGAGGAGG 
ACCACCCGGA GTCTGCAAGT ACGCCCTGTC TCTATGCCTG TTGACAAGAG CCTTCTGGGG 
GCTTTGTCGC TGCCCATATC TGAAGCAGCA AAAGACACGG ATGGAAAAGA AAACTGTCTG 
GATCTGAATA GGAAGCAGCG CAAGCAGGAG ACAAGGATGA CAGAAGAAAG GGAGCCGCAA 
CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC TCCAGTGCGC 
TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA GAACAGTGTG 
GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501541.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501604.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X15, mRNA.

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CloneID	OMu31955
Clone ID Related Accession (Same CDS sequence)	XM_006501541.3 , XM_006501541.4
Accession Version	XM_006501541.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501541.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGATC TGAATAGGAA GCAGCGCAAG 
GAGACAAGGA TGACAGAAGA AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT 
TCAACAAAAA GTTATCCTCC TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC 
CAACTGCCAG TGTATCAAAC CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA 
TCAAACCAAA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501604.1
CDS	626..3880
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLDLNRKQRKETRMTEEREPQLEKTHIFQISTKSYPPPPVRSSTKMPTKGQLPVYQTQNSVEDEVQRVRGSNQI

Target ORF information:

RefSeq Version	XM_006501541.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501541.3

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGATC TGAATAGGAA GCAGCGCAAG 
GAGACAAGGA TGACAGAAGA AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT 
TCAACAAAAA GTTATCCTCC TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC 
CAACTGCCAG TGTATCAAAC CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA 
TCAAACCAAA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_011240131.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238433.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X2, mRNA.

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CloneID	OMu31948
Clone ID Related Accession (Same CDS sequence)	XM_011240131.3 , XM_006501533.4 , XM_006501530.3 , XM_006501531.3 , XM_017319593.1 , XM_006501530.4 , XM_006501531.4 , XM_006501533.3 , XM_017319593.2 , XM_017319594.1 , XM_011240131.2
Accession Version	XM_011240131.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240131.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238433.1
CDS	492..5576
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_011240131.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240131.3

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501533.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501596.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X6, mRNA.

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CloneID	OMu31948
Clone ID Related Accession (Same CDS sequence)	XM_011240131.3 , XM_006501533.4 , XM_006501530.3 , XM_006501531.3 , XM_017319593.1 , XM_006501530.4 , XM_006501531.4 , XM_006501533.3 , XM_017319593.2 , XM_017319594.1 , XM_011240131.2
Accession Version	XM_006501533.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501533.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501596.1
CDS	316..5400
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006501533.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501533.4

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501530.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501593.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X1, mRNA.

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CloneID	OMu31948
Clone ID Related Accession (Same CDS sequence)	XM_011240131.3 , XM_006501533.4 , XM_006501530.3 , XM_006501531.3 , XM_017319593.1 , XM_006501530.4 , XM_006501531.4 , XM_006501533.3 , XM_017319593.2 , XM_017319594.1 , XM_011240131.2
Accession Version	XM_006501530.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501530.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501593.1
CDS	402..5486
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006501530.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501530.4

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501531.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501594.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X4, mRNA.

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CloneID	OMu31948
Clone ID Related Accession (Same CDS sequence)	XM_011240131.3 , XM_006501533.4 , XM_006501530.3 , XM_006501531.3 , XM_017319593.1 , XM_006501530.4 , XM_006501531.4 , XM_006501533.3 , XM_017319593.2 , XM_017319594.1 , XM_011240131.2
Accession Version	XM_006501531.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501531.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501594.1
CDS	1736..6820
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006501531.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501531.4

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319593.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175082.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu31948
Clone ID Related Accession (Same CDS sequence)	XM_011240131.3 , XM_006501533.4 , XM_006501530.3 , XM_006501531.3 , XM_017319593.1 , XM_006501530.4 , XM_006501531.4 , XM_006501533.3 , XM_017319593.2 , XM_017319594.1 , XM_011240131.2
Accession Version	XM_017319593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175082.1
CDS	530..5614
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_017319593.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319593.2

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501535.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501598.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X7, mRNA.

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CloneID	OMu31949
Clone ID Related Accession (Same CDS sequence)	XM_006501535.3 , XM_006501535.4
Accession Version	XM_006501535.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501535.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGT GGAGTCCTTT AATCCTCAAC 
CCCAGTTATA CCATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGCAGGAG ACAAGGATGA CAGAAGAAAG 
GGAGCCGCAA CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC 
TCCAGTGCGC TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA 
GAACAGTGTG GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GACAAATGAT 
TGCCAGGAAA ACCACAATCC CCCGAAATTC CTTTCTCCAA GGAAGCATTT GGCTCTGGAT 
CCTGCAACGA AGGGACTGCA AGAGAGACTG CATGGAATGA AAACCAATGA GAAGGAGCAT 
GCCGAGGGCT TCTTGGGAGA GAAAAGCATG CTGTCCGGAA GCGTTCTTTC TCAAAGTTCT 
CTGGAGGTAG AGAACCTGGA AGGCAGCAGA GCCAAGGGCA GAGCTGCAAC CTCCTTTTCT 
CTCTCGGACG TCTCCGCGCT CTGTTCTGAC ATCCCTGATT TACATTCAAC TGCCATTCTA 
CAGGACACTG AAATTTCCAA TCTCATTGAT GACGTGACTT TAACAAATGA GAATCAGTCG 
GGAAGTTCCA TCTCGGCACT GATTGGCCAG TTTGAAGAAA GCAACCACCC GGCTAACGTT 
ACTGTGGTTT CTCATCTCAG CACCAGTGGG GCATCAGGAA GTGCTCCTTT CCAAACACCC 
TTCAAGCATG GTCTTTCCCA GGGGAACCAG AAGGCCTCCT TCTTGTGCTC ATCTCCTGAG 
CTGAATAAGC TCTCTAGTGT TGAGACCACC AAACTGGCAA ATAACGCAGT TCCTTGTGGA 
GTGATTGGTT CTCCCATCTC TACACCAAAG CCAGGTGATG ACCCTTCAGA TAAGGCCAAG 
ACAAGAGTCA TTGAAGGCAA CCTGCCTGGG TTCCCTGATG CTTCTCCTGG TCAATTTCCC 
AAAAGCCCCA CCCATGGAGA GGACCATAGT CAAGTGATGA ACAGCCCTGC TCTCTCCACG 
GAGTTGGCCA TAGAAGATAT AATTGCAGAC CCTGCTCTCT CTATAAATTC TGCAGAAAGC 
AGTCTTGTGG AAATTGATGG AGAATCTGAA AACCTATCTC TAACAACCTG TGACTACAGG 
GAAGAAGCTC CAAGTCAACT TGTTTCTCCA CTAAAACTGC AGCAGAGCCA GGAGATGGTG 
GAACACATTC AGAGGGGCTT GAGGAATGGC TACTGTAAAG AGACTCTCCT CCCTTCTGAA 
ATATTCAACA ATATTCCAGG TGTCAAAAAT CACAGTATTT CTCATCTAAC CTATCAGGGT 
GCTGGCTTTG TGTATAACCA TTTCTCAAGT TCAGATGCAA AAACGAACCA AATCTGTGAG 
CCCCAGCAGC CTAGGGCTCC AGATATGCAT GCCCCTACAC CTACACCATC AACACATGCT 
CCTTTGGCTG CTTTGAAACT GCCAAGCCCA TGCAAATCCA AAAGTCTGGG GGACTTAACA 
TCAGAGGACA TTGCCTGCAA TTTTGAAAGC AAATATCAAT GTATTAGTAG GAGCTTTGTG 
ACAAACGGCA TTAGGGACAA GAGCGTGACT ATGAAGACAA AGTCATTAGA GCCTTTAGAC 
GCCCTGACTG AACAGCTCCG GAAGCTGGTG TCCTTTGACC AAGAAGACAG CTGTCAAGTG 
CTCTACTCAA AGCAGGATGT CAATCAGTGT CCCAGGGCAT TAGTCAGAAA GTTGTCGTCT 
AGAAGTCAGA GCAGAGTGCG CAACATTGCT AGCCGTGCCA AGGAGAAGCA GGAAGCTGGC 
AAGCAAAAAG CTATGGCCCA GAGCACCAGA GGAGGAGTGG TCCTTAGAAG TAAACCGCCC 
GCTCCTGCTC TGGCCGTGAA CCGTCACTCC ACGGGCTCAT ACATTGCAAG CTACCTGAGG 
AACATGAAAG CTGGTGGCCT AGAGGGTCGA GGCATCCCAG AGGGAGCGTG CACGGCCCTT 
CGCTATGGCT ACATGGACCA GTTTTGTTCA GATAATTCAG TTCTGCAGAC TGAGCCAAGC 
AGCGAAGATA AACCCGAAAT TTATTTTCTT TTGAGGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501598.1
CDS	402..5483
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006501535.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501535.4

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGT GGAGTCCTTT AATCCTCAAC 
CCCAGTTATA CCATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGCAGGAG ACAAGGATGA CAGAAGAAAG 
GGAGCCGCAA CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC 
TCCAGTGCGC TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA 
GAACAGTGTG GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GACAAATGAT 
TGCCAGGAAA ACCACAATCC CCCGAAATTC CTTTCTCCAA GGAAGCATTT GGCTCTGGAT 
CCTGCAACGA AGGGACTGCA AGAGAGACTG CATGGAATGA AAACCAATGA GAAGGAGCAT 
GCCGAGGGCT TCTTGGGAGA GAAAAGCATG CTGTCCGGAA GCGTTCTTTC TCAAAGTTCT 
CTGGAGGTAG AGAACCTGGA AGGCAGCAGA GCCAAGGGCA GAGCTGCAAC CTCCTTTTCT 
CTCTCGGACG TCTCCGCGCT CTGTTCTGAC ATCCCTGATT TACATTCAAC TGCCATTCTA 
CAGGACACTG AAATTTCCAA TCTCATTGAT GACGTGACTT TAACAAATGA GAATCAGTCG 
GGAAGTTCCA TCTCGGCACT GATTGGCCAG TTTGAAGAAA GCAACCACCC GGCTAACGTT 
ACTGTGGTTT CTCATCTCAG CACCAGTGGG GCATCAGGAA GTGCTCCTTT CCAAACACCC 
TTCAAGCATG GTCTTTCCCA GGGGAACCAG AAGGCCTCCT TCTTGTGCTC ATCTCCTGAG 
CTGAATAAGC TCTCTAGTGT TGAGACCACC AAACTGGCAA ATAACGCAGT TCCTTGTGGA 
GTGATTGGTT CTCCCATCTC TACACCAAAG CCAGGTGATG ACCCTTCAGA TAAGGCCAAG 
ACAAGAGTCA TTGAAGGCAA CCTGCCTGGG TTCCCTGATG CTTCTCCTGG TCAATTTCCC 
AAAAGCCCCA CCCATGGAGA GGACCATAGT CAAGTGATGA ACAGCCCTGC TCTCTCCACG 
GAGTTGGCCA TAGAAGATAT AATTGCAGAC CCTGCTCTCT CTATAAATTC TGCAGAAAGC 
AGTCTTGTGG AAATTGATGG AGAATCTGAA AACCTATCTC TAACAACCTG TGACTACAGG 
GAAGAAGCTC CAAGTCAACT TGTTTCTCCA CTAAAACTGC AGCAGAGCCA GGAGATGGTG 
GAACACATTC AGAGGGGCTT GAGGAATGGC TACTGTAAAG AGACTCTCCT CCCTTCTGAA 
ATATTCAACA ATATTCCAGG TGTCAAAAAT CACAGTATTT CTCATCTAAC CTATCAGGGT 
GCTGGCTTTG TGTATAACCA TTTCTCAAGT TCAGATGCAA AAACGAACCA AATCTGTGAG 
CCCCAGCAGC CTAGGGCTCC AGATATGCAT GCCCCTACAC CTACACCATC AACACATGCT 
CCTTTGGCTG CTTTGAAACT GCCAAGCCCA TGCAAATCCA AAAGTCTGGG GGACTTAACA 
TCAGAGGACA TTGCCTGCAA TTTTGAAAGC AAATATCAAT GTATTAGTAG GAGCTTTGTG 
ACAAACGGCA TTAGGGACAA GAGCGTGACT ATGAAGACAA AGTCATTAGA GCCTTTAGAC 
GCCCTGACTG AACAGCTCCG GAAGCTGGTG TCCTTTGACC AAGAAGACAG CTGTCAAGTG 
CTCTACTCAA AGCAGGATGT CAATCAGTGT CCCAGGGCAT TAGTCAGAAA GTTGTCGTCT 
AGAAGTCAGA GCAGAGTGCG CAACATTGCT AGCCGTGCCA AGGAGAAGCA GGAAGCTGGC 
AAGCAAAAAG CTATGGCCCA GAGCACCAGA GGAGGAGTGG TCCTTAGAAG TAAACCGCCC 
GCTCCTGCTC TGGCCGTGAA CCGTCACTCC ACGGGCTCAT ACATTGCAAG CTACCTGAGG 
AACATGAAAG CTGGTGGCCT AGAGGGTCGA GGCATCCCAG AGGGAGCGTG CACGGCCCTT 
CGCTATGGCT ACATGGACCA GTTTTGTTCA GATAATTCAG TTCTGCAGAC TGAGCCAAGC 
AGCGAAGATA AACCCGAAAT TTATTTTCTT TTGAGGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501536.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501599.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X8, mRNA.

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CloneID	OMu31950
Clone ID Related Accession (Same CDS sequence)	XM_006501536.3 , XM_006501536.4
Accession Version	XM_006501536.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501536.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGGAG ACAAGGATGA CAGAAGAAAG 
GGAGCCGCAA CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC 
TCCAGTGCGC TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA 
GAACAGTGTG GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GACAAATGAT 
TGCCAGGAAA ACCACAATCC CCCGAAATTC CTTTCTCCAA GGAAGCATTT GGCTCTGGAT 
CCTGCAACGA AGGGACTGCA AGAGAGACTG CATGGAATGA AAACCAATGA GAAGGAGCAT 
GCCGAGGGCT TCTTGGGAGA GAAAAGCATG CTGTCCGGAA GCGTTCTTTC TCAAAGTTCT 
CTGGAGGTAG AGAACCTGGA AGGCAGCAGA GCCAAGGGCA GAGCTGCAAC CTCCTTTTCT 
CTCTCGGACG TCTCCGCGCT CTGTTCTGAC ATCCCTGATT TACATTCAAC TGCCATTCTA 
CAGGACACTG AAATTTCCAA TCTCATTGAT GACGTGACTT TAACAAATGA GAATCAGTCG 
GGAAGTTCCA TCTCGGCACT GATTGGCCAG TTTGAAGAAA GCAACCACCC GGCTAACGTT 
ACTGTGGTTT CTCATCTCAG CACCAGTGGG GCATCAGGAA GTGCTCCTTT CCAAACACCC 
TTCAAGCATG GTCTTTCCCA GGGGAACCAG AAGGCCTCCT TCTTGTGCTC ATCTCCTGAG 
CTGAATAAGC TCTCTAGTGT TGAGACCACC AAACTGGCAA ATAACGCAGT TCCTTGTGGA 
GTGATTGGTT CTCCCATCTC TACACCAAAG CCAGGTGATG ACCCTTCAGA TAAGGCCAAG 
ACAAGAGTCA TTGAAGGCAA CCTGCCTGGG TTCCCTGATG CTTCTCCTGG TCAATTTCCC 
AAAAGCCCCA CCCATGGAGA GGACCATAGT CAAGTGATGA ACAGCCCTGC TCTCTCCACG 
GAGTTGGCCA TAGAAGATAT AATTGCAGAC CCTGCTCTCT CTATAAATTC TGCAGAAAGC 
AGTCTTGTGG AAATTGATGG AGAATCTGAA AACCTATCTC TAACAACCTG TGACTACAGG 
GAAGAAGCTC CAAGTCAACT TGTTTCTCCA CTAAAACTGC AGCAGAGCCA GGAGATGGTG 
GAACACATTC AGAGGGGCTT GAGGAATGGC TACTGTAAAG AGACTCTCCT CCCTTCTGAA 
ATATTCAACA ATATTCCAGG TGTCAAAAAT CACAGTATTT CTCATCTAAC CTATCAGGGT 
GCTGGCTTTG TGTATAACCA TTTCTCAAGT TCAGATGCAA AAACGAACCA AATCTGTGAG 
CCCCAGCAGC CTAGGGCTCC AGATATGCAT GCCCCTACAC CTACACCATC AACACATGCT 
CCTTTGGCTG CTTTGAAACT GCCAAGCCCA TGCAAATCCA AAAGTCTGGG GGACTTAACA 
TCAGAGGACA TTGCCTGCAA TTTTGAAAGC AAATATCAAT GTATTAGTAG GAGCTTTGTG 
ACAAACGGCA TTAGGGACAA GAGCGTGACT ATGAAGACAA AGTCATTAGA GCCTTTAGAC 
GCCCTGACTG AACAGCTCCG GAAGCTGGTG TCCTTTGACC AAGAAGACAG CTGTCAAGTG 
CTCTACTCAA AGCAGGATGT CAATCAGTGT CCCAGGGCAT TAGTCAGAAA GTTGTCGTCT 
AGAAGTCAGA GCAGAGTGCG CAACATTGCT AGCCGTGCCA AGGAGAAGCA GGAAGCTGGC 
AAGCAAAAAG CTATGGCCCA GAGCACCAGA GGAGGAGTGG TCCTTAGAAG TAAACCGCCC 
GCTCCTGCTC TGGCCGTGAA CCGTCACTCC ACGGGCTCAT ACATTGCAAG CTACCTGAGG 
AACATGAAAG CTGGTGGCCT AGAGGGTCGA GGCATCCCAG AGGGAGCGTG CACGGCCCTT 
CGCTATGGCT ACATGGACCA GTTTTGTTCA GATAATTCAG TTCTGCAGAC TGAGCCAAGC 
AGCGAAGATA AACCCGAAAT TTATTTTCTT TTGAGGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501599.1
CDS	402..5483
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006501536.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501536.4

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGGAG ACAAGGATGA CAGAAGAAAG 
GGAGCCGCAA CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC 
TCCAGTGCGC TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA 
GAACAGTGTG GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GACAAATGAT 
TGCCAGGAAA ACCACAATCC CCCGAAATTC CTTTCTCCAA GGAAGCATTT GGCTCTGGAT 
CCTGCAACGA AGGGACTGCA AGAGAGACTG CATGGAATGA AAACCAATGA GAAGGAGCAT 
GCCGAGGGCT TCTTGGGAGA GAAAAGCATG CTGTCCGGAA GCGTTCTTTC TCAAAGTTCT 
CTGGAGGTAG AGAACCTGGA AGGCAGCAGA GCCAAGGGCA GAGCTGCAAC CTCCTTTTCT 
CTCTCGGACG TCTCCGCGCT CTGTTCTGAC ATCCCTGATT TACATTCAAC TGCCATTCTA 
CAGGACACTG AAATTTCCAA TCTCATTGAT GACGTGACTT TAACAAATGA GAATCAGTCG 
GGAAGTTCCA TCTCGGCACT GATTGGCCAG TTTGAAGAAA GCAACCACCC GGCTAACGTT 
ACTGTGGTTT CTCATCTCAG CACCAGTGGG GCATCAGGAA GTGCTCCTTT CCAAACACCC 
TTCAAGCATG GTCTTTCCCA GGGGAACCAG AAGGCCTCCT TCTTGTGCTC ATCTCCTGAG 
CTGAATAAGC TCTCTAGTGT TGAGACCACC AAACTGGCAA ATAACGCAGT TCCTTGTGGA 
GTGATTGGTT CTCCCATCTC TACACCAAAG CCAGGTGATG ACCCTTCAGA TAAGGCCAAG 
ACAAGAGTCA TTGAAGGCAA CCTGCCTGGG TTCCCTGATG CTTCTCCTGG TCAATTTCCC 
AAAAGCCCCA CCCATGGAGA GGACCATAGT CAAGTGATGA ACAGCCCTGC TCTCTCCACG 
GAGTTGGCCA TAGAAGATAT AATTGCAGAC CCTGCTCTCT CTATAAATTC TGCAGAAAGC 
AGTCTTGTGG AAATTGATGG AGAATCTGAA AACCTATCTC TAACAACCTG TGACTACAGG 
GAAGAAGCTC CAAGTCAACT TGTTTCTCCA CTAAAACTGC AGCAGAGCCA GGAGATGGTG 
GAACACATTC AGAGGGGCTT GAGGAATGGC TACTGTAAAG AGACTCTCCT CCCTTCTGAA 
ATATTCAACA ATATTCCAGG TGTCAAAAAT CACAGTATTT CTCATCTAAC CTATCAGGGT 
GCTGGCTTTG TGTATAACCA TTTCTCAAGT TCAGATGCAA AAACGAACCA AATCTGTGAG 
CCCCAGCAGC CTAGGGCTCC AGATATGCAT GCCCCTACAC CTACACCATC AACACATGCT 
CCTTTGGCTG CTTTGAAACT GCCAAGCCCA TGCAAATCCA AAAGTCTGGG GGACTTAACA 
TCAGAGGACA TTGCCTGCAA TTTTGAAAGC AAATATCAAT GTATTAGTAG GAGCTTTGTG 
ACAAACGGCA TTAGGGACAA GAGCGTGACT ATGAAGACAA AGTCATTAGA GCCTTTAGAC 
GCCCTGACTG AACAGCTCCG GAAGCTGGTG TCCTTTGACC AAGAAGACAG CTGTCAAGTG 
CTCTACTCAA AGCAGGATGT CAATCAGTGT CCCAGGGCAT TAGTCAGAAA GTTGTCGTCT 
AGAAGTCAGA GCAGAGTGCG CAACATTGCT AGCCGTGCCA AGGAGAAGCA GGAAGCTGGC 
AAGCAAAAAG CTATGGCCCA GAGCACCAGA GGAGGAGTGG TCCTTAGAAG TAAACCGCCC 
GCTCCTGCTC TGGCCGTGAA CCGTCACTCC ACGGGCTCAT ACATTGCAAG CTACCTGAGG 
AACATGAAAG CTGGTGGCCT AGAGGGTCGA GGCATCCCAG AGGGAGCGTG CACGGCCCTT 
CGCTATGGCT ACATGGACCA GTTTTGTTCA GATAATTCAG TTCTGCAGAC TGAGCCAAGC 
AGCGAAGATA AACCCGAAAT TTATTTTCTT TTGAGGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501538.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501601.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X9, mRNA.

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CloneID	OMu31952
Clone ID Related Accession (Same CDS sequence)	XM_006501538.4 , XM_006501538.3
Accession Version	XM_006501538.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501538.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGCAGGAG ACAAGGATGA CAGAAGAAAG 
GGAGCCGCAA CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC 
TCCAGTGCGC TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA 
GAACAGTGTG GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GACAAATGAT 
TGCCAGGAAA ACCACAATCC CCCGAAATTC CTTTCTCCAA GGAAGCATTT GGCTCTGGAT 
CCTGCAACGA AGGGACTGCA AGAGAGACTG CATGGAATGA AAACCAATGA GAAGGAGCAT 
GCCGAGGGCT TCTTGGGAGA GAAAAGCATG CTGTCCGGAA GCGTTCTTTC TCAAAGTTCT 
CTGGAGGTAG AGAACCTGGA AGGCAGCAGA GCCAAGGGCA GAGCTGCAAC CTCCTTTTCT 
CTCTCGGACG TCTCCGCGCT CTGTTCTGAC ATCCCTGATT TACATTCAAC TGCCATTCTA 
CAGGACACTG AAATTTCCAA TCTCATTGAT GACGTGACTT TAACAAATGA GAATCAGTCG 
GGAAGTTCCA TCTCGGCACT GATTGGCCAG TTTGAAGAAA GCAACCACCC GGCTAACGTT 
ACTGTGGTTT CTCATCTCAG CACCAGTGGG GCATCAGGAA GTGCTCCTTT CCAAACACCC 
TTCAAGCATG GTCTTTCCCA GGGGAACCAG AAGGCCTCCT TCTTGTGCTC ATCTCCTGAG 
CTGAATAAGC TCTCTAGTGT TGAGACCACC AAACTGGCAA ATAACGCAGT TCCTTGTGGA 
GTGATTGGTT CTCCCATCTC TACACCAAAG CCAGGTGATG ACCCTTCAGA TAAGGCCAAG 
ACAAGAGTCA TTGAAGGCAA CCTGCCTGGG TTCCCTGATG CTTCTCCTGG TCAATTTCCC 
AAAAGCCCCA CCCATGGAGA GGACCATAGT CAAGTGATGA ACAGCCCTGC TCTCTCCACG 
GAGTTGGCCA TAGAAGATAT AATTGCAGAC CCTGCTCTCT CTATAAATTC TGCAGAAAGC 
AGTCTTGTGG AAATTGATGG AGAATCTGAA AACCTATCTC TAACAACCTG TGACTACAGG 
GAAGAAGCTC CAAGTCAACT TGTTTCTCCA CTAAAACTGC AGCAGAGCCA GGAGATGGTG 
GAACACATTC AGAGGGGCTT GAGGAATGGC TACTGTAAAG AGACTCTCCT CCCTTCTGAA 
ATATTCAACA ATATTCCAGG TGTCAAAAAT CACAGTATTT CTCATCTAAC CTATCAGGGT 
GCTGGCTTTG TGTATAACCA TTTCTCAAGT TCAGATGCAA AAACGAACCA AATCTGTGAG 
CCCCAGCAGC CTAGGGCTCC AGATATGCAT GCCCCTACAC CTACACCATC AACACATGCT 
CCTTTGGCTG CTTTGAAACT GCCAAGCCCA TGCAAATCCA AAAGTCTGGG GGACTTAACA 
TCAGAGGACA TTGCCTGCAA TTTTGAAAGC AAATATCAAT GTATTAGTAG GAGCTTTGTG 
ACAAACGGCA TTAGGGACAA GAGCGTGACT ATGAAGACAA AGTCATTAGA GCCTTTAGAC 
GCCCTGACTG AACAGCTCCG GAAGCTGGTG TCCTTTGACC AAGAAGACAG CTGTCAAGTG 
CTCTACTCAA AGCAGGATGT CAATCAGTGT CCCAGGGCAT TAGTCAGAAA GTTGTCGTCT 
AGAAGTCAGA GCAGAGTGCG CAACATTGCT AGCCGTGCCA AGGAGAAGCA GGAAGCTGGC 
AAGCAAAAAG CTATGGCCCA GAGCACCAGA GGAGGAGTGG TCCTTAGAAG TAAACCGCCC 
GCTCCTGCTC TGGCCGTGAA CCGTCACTCC ACGGGCTCAT ACATTGCAAG CTACCTGAGG 
AACATGAAAG CTGGTGGCCT AGAGGGTCGA GGCATCCCAG AGGGAGCGTG CACGGCCCTT 
CGCTATGGCT ACATGGACCA GTTTTGTTCA GATAATTCAG TTCTGCAGAC TGAGCCAAGC 
AGCGAAGATA AACCCGAAAT TTATTTTCTT TTGAGGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501601.1
CDS	402..5423
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006501538.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501538.4

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGCAGGAG ACAAGGATGA CAGAAGAAAG 
GGAGCCGCAA CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC 
TCCAGTGCGC TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA 
GAACAGTGTG GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GACAAATGAT 
TGCCAGGAAA ACCACAATCC CCCGAAATTC CTTTCTCCAA GGAAGCATTT GGCTCTGGAT 
CCTGCAACGA AGGGACTGCA AGAGAGACTG CATGGAATGA AAACCAATGA GAAGGAGCAT 
GCCGAGGGCT TCTTGGGAGA GAAAAGCATG CTGTCCGGAA GCGTTCTTTC TCAAAGTTCT 
CTGGAGGTAG AGAACCTGGA AGGCAGCAGA GCCAAGGGCA GAGCTGCAAC CTCCTTTTCT 
CTCTCGGACG TCTCCGCGCT CTGTTCTGAC ATCCCTGATT TACATTCAAC TGCCATTCTA 
CAGGACACTG AAATTTCCAA TCTCATTGAT GACGTGACTT TAACAAATGA GAATCAGTCG 
GGAAGTTCCA TCTCGGCACT GATTGGCCAG TTTGAAGAAA GCAACCACCC GGCTAACGTT 
ACTGTGGTTT CTCATCTCAG CACCAGTGGG GCATCAGGAA GTGCTCCTTT CCAAACACCC 
TTCAAGCATG GTCTTTCCCA GGGGAACCAG AAGGCCTCCT TCTTGTGCTC ATCTCCTGAG 
CTGAATAAGC TCTCTAGTGT TGAGACCACC AAACTGGCAA ATAACGCAGT TCCTTGTGGA 
GTGATTGGTT CTCCCATCTC TACACCAAAG CCAGGTGATG ACCCTTCAGA TAAGGCCAAG 
ACAAGAGTCA TTGAAGGCAA CCTGCCTGGG TTCCCTGATG CTTCTCCTGG TCAATTTCCC 
AAAAGCCCCA CCCATGGAGA GGACCATAGT CAAGTGATGA ACAGCCCTGC TCTCTCCACG 
GAGTTGGCCA TAGAAGATAT AATTGCAGAC CCTGCTCTCT CTATAAATTC TGCAGAAAGC 
AGTCTTGTGG AAATTGATGG AGAATCTGAA AACCTATCTC TAACAACCTG TGACTACAGG 
GAAGAAGCTC CAAGTCAACT TGTTTCTCCA CTAAAACTGC AGCAGAGCCA GGAGATGGTG 
GAACACATTC AGAGGGGCTT GAGGAATGGC TACTGTAAAG AGACTCTCCT CCCTTCTGAA 
ATATTCAACA ATATTCCAGG TGTCAAAAAT CACAGTATTT CTCATCTAAC CTATCAGGGT 
GCTGGCTTTG TGTATAACCA TTTCTCAAGT TCAGATGCAA AAACGAACCA AATCTGTGAG 
CCCCAGCAGC CTAGGGCTCC AGATATGCAT GCCCCTACAC CTACACCATC AACACATGCT 
CCTTTGGCTG CTTTGAAACT GCCAAGCCCA TGCAAATCCA AAAGTCTGGG GGACTTAACA 
TCAGAGGACA TTGCCTGCAA TTTTGAAAGC AAATATCAAT GTATTAGTAG GAGCTTTGTG 
ACAAACGGCA TTAGGGACAA GAGCGTGACT ATGAAGACAA AGTCATTAGA GCCTTTAGAC 
GCCCTGACTG AACAGCTCCG GAAGCTGGTG TCCTTTGACC AAGAAGACAG CTGTCAAGTG 
CTCTACTCAA AGCAGGATGT CAATCAGTGT CCCAGGGCAT TAGTCAGAAA GTTGTCGTCT 
AGAAGTCAGA GCAGAGTGCG CAACATTGCT AGCCGTGCCA AGGAGAAGCA GGAAGCTGGC 
AAGCAAAAAG CTATGGCCCA GAGCACCAGA GGAGGAGTGG TCCTTAGAAG TAAACCGCCC 
GCTCCTGCTC TGGCCGTGAA CCGTCACTCC ACGGGCTCAT ACATTGCAAG CTACCTGAGG 
AACATGAAAG CTGGTGGCCT AGAGGGTCGA GGCATCCCAG AGGGAGCGTG CACGGCCCTT 
CGCTATGGCT ACATGGACCA GTTTTGTTCA GATAATTCAG TTCTGCAGAC TGAGCCAAGC 
AGCGAAGATA AACCCGAAAT TTATTTTCTT TTGAGGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319595.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175084.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X11, mRNA.

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CloneID	OMu80626
Clone ID Related Accession (Same CDS sequence)	XM_017319595.2 , XM_017319595.1
Accession Version	XM_017319595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AACCTGGATG TAAAGGAAAG TGGAAAGAAG 
TCCCATGGGC GATCCCTGAT GGCCAACTTT GGGAAACACA AGCAGAAAGC TACGAAATCA 
CGTTCTAAAT CCTATAGTAC TGATGATGAG GATGATAGCC TTCAGAATCC TGGCAAGGAG 
GGAGGCCAGC TGTACAGGCT GGGCCGCCGA AGGAGAACCA TGAAACTCTG CAGAGAGCTC 
TCGGACTTGG TGGTGTACAC AAACTCCGTG GCAGCCCAGG ATATTGTTGA TGATGGAACC 
ACAGGGAATG TGTTGTCCTT CAGCGAAACA AGAGCCCATC AAGTGGTTCA GCAGAAGTCA 
GAGCAGTTCA TGATCTACAA CCAAAAACAG CTCACAAGGA TTTACCCCTC TGCCTACCGC 
ATCGATTCCA GTAACTTCAA CCCACTGCCC TACTGGAATG CAGGCTGCCA GCTCGTGGCT 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGATCAATC GAGCAAAATT CAAGGCGAAT 
GGCAACTGTG GCTATATTCT CAAGCCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGACC CACTTCCTGC CAACCCCAAA AAACAGCTCA TACTGAAAGT GATCAGTGGA 
CAGCAACTCC CCAAACCTCC TGACTCTATG TTTGGAGACC GAGGCGAGAT CATTGACCCT 
TTCGTTGAAG TTGAAATTAT TGGGCTGCCA GTAGACTGTT GCAAGGACCA AACTCGTGTG 
GTGGATGACA ACGGATTTAA CCCTGTGTGG GAAGAAACCC TAACTTTTAC AGTTCACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCTATTGG AAGAGACTTT 
GTCGGACAAA GGACTGTGAC CTTCAGTAGC CTCGTGCCCG GCTACCGGCA TGTCTACCTG 
GAAGGACTCA CAGAAGCATC TATTTTCGTT CACATAACCA TCAATGAGAT CTTTGGAAAG 
TGGAGTCCTT TAATCCTCAA CCCCAGTTAT ACCATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGCCT GAAGGGGCTG TTCAATAAGA ATCCCAGGCA CGCTTCTTCA 
GAAAACAACT CGCATTATGT TCGGAAGCGA TCGATTGGAG ATAGGATTCT GCGACGTACA 
GCTAGTGCTC CAGCCAAAGG CAGGAAAAAG AGCAAAGTGG GCTTCCAAGA AATGGTTGAG 
ATAAAAGATT CTGTCTCTGA GGCTTCAAGA GATCAAGATG GTGTCCTGAG GAGGACCACC 
CGGAGTCTGC AAGTACGCCC TGTCTCTATG CCTGTTGACA AGAGCCTTCT GGGGGCTTTG 
TCGCTGCCCA TATCTGAAGC AGCAAAAGAC ACGGATGGAA AAGAAAACTG TCTGGCAGGA 
GACAAGGATG ACAGAAGAAA GGGAGCCGCA ACTAGAAAAG ACCCACATTT TTCAAATTTC 
AACAAAAAGT TATCCTCCTC CTCCAGTGCG CTCCTCCACA AAGATGCCAA CCAAGGGCCA 
ACTGCCAGTG TATCAAACCC AGAACAGTGT GGAGGACGAG GTGCAAAGAG TGAGAGGATC 
AAACCAAATA TGACAAATGA TTGCCAGGAA AACCACAATC CCCCGAAATT CCTTTCTCCA 
AGGAAGCATT TGGCTCTGGA TCCTGCAACG AAGGGACTGC AAGAGAGACT GCATGGAATG 
AAAACCAATG AGAAGGAGCA TGCCGAGGGC TTCTTGGGAG AGAAAAGCAT GCTGTCCGGA 
AGCGTTCTTT CTCAAAGTTC TCTGGAGGTA GAGAACCTGG AAGGCAGCAG AGCCAAGGGC 
AGAGCTGCAA CCTCCTTTTC TCTCTCGGAC GTCTCCGCGC TCTGTTCTGA CATCCCTGAT 
TTACATTCAA CTGCCATTCT ACAGGACACT GAAATTTCCA ATCTCATTGA TGACGTGACT 
TTAACAAATG AGAATCAGTC GGGAAGTTCC ATCTCGGCAC TGATTGGCCA GTTTGAAGAA 
AGCAACCACC CGGCTAACGT TACTGTGGTT TCTCATCTCA GCACCAGTGG GGCATCAGGA 
AGTGCTCCTT TCCAAACACC CTTCAAGCAT GGTCTTTCCC AGGGGAACCA GAAGGCCTCC 
TTCTTGTGCT CATCTCCTGA GCTGAATAAG CTCTCTAGTG TTGAGACCAC CAAACTGGCA 
AATAACGCAG TTCCTTGTGG AGTGATTGGT TCTCCCATCT CTACACCAAA GCCAGGTGAT 
GACCCTTCAG ATAAGGCCAA GACAAGAGTC ATTGAAGGCA ACCTGCCTGG GTTCCCTGAT 
GCTTCTCCTG GTCAATTTCC CAAAAGCCCC ACCCATGGAG AGGACCATAG TCAAGTGATG 
AACAGCCCTG CTCTCTCCAC GGAGTTGGCC ATAGAAGATA TAATTGCAGA CCCTGCTCTC 
TCTATAAATT CTGCAGAAAG CAGTCTTGTG GAAATTGATG GAGAATCTGA AAACCTATCT 
CTAACAACCT GTGACTACAG GGAAGAAGCT CCAAGTCAAC TTGTTTCTCC ACTAAAACTG 
CAGCAGAGCC AGGAGATGGT GGAACACATT CAGAGGGGCT TGAGGAATGG CTACTGTAAA 
GAGACTCTCC TCCCTTCTGA AATATTCAAC AATATTCCAG GTGTCAAAAA TCACAGTATT 
TCTCATCTAA CCTATCAGGG TGCTGGCTTT GTGTATAACC ATTTCTCAAG TTCAGATGCA 
AAAACGAACC AAATCTGTGA GCCCCAGCAG CCTAGGGCTC CAGATATGCA TGCCCCTACA 
CCTACACCAT CAACACATGC TCCTTTGGCT GCTTTGAAAC TGCCAAGCCC ATGCAAATCC 
AAAAGTCTGG GGGACTTAAC ATCAGAGGAC ATTGCCTGCA ATTTTGAAAG CAAATATCAA 
TGTATTAGTA GGAGCTTTGT GACAAACGGC ATTAGGGACA AGAGCGTGAC TATGAAGACA 
AAGTCATTAG AGCCTTTAGA CGCCCTGACT GAACAGCTCC GGAAGCTGGT GTCCTTTGAC 
CAAGAAGACA GCTGTCAAGT GCTCTACTCA AAGCAGGATG TCAATCAGTG TCCCAGGGCA 
TTAGTCAGAA AGTTGTCGTC TAGAAGTCAG AGCAGAGTGC GCAACATTGC TAGCCGTGCC 
AAGGAGAAGC AGGAAGCTGG CAAGCAAAAA GCTATGGCCC AGAGCACCAG AGGAGGAGTG 
GTCCTTAGAA GTAAACCGCC CGCTCCTGCT CTGGCCGTGA ACCGTCACTC CACGGGCTCA 
TACATTGCAA GCTACCTGAG GAACATGAAA GCTGGTGGCC TAGAGGGTCG AGGCATCCCA 
GAGGGAGCGT GCACGGCCCT TCGCTATGGC TACATGGACC AGTTTTGTTC AGATAATTCA 
GTTCTGCAGA CTGAGCCAAG CAGCGAAGAT AAACCCGAAA TTTATTTTCT TTTGAGGCTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175084.1
CDS	402..5324
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_017319595.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319595.2

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AACCTGGATG TAAAGGAAAG TGGAAAGAAG 
TCCCATGGGC GATCCCTGAT GGCCAACTTT GGGAAACACA AGCAGAAAGC TACGAAATCA 
CGTTCTAAAT CCTATAGTAC TGATGATGAG GATGATAGCC TTCAGAATCC TGGCAAGGAG 
GGAGGCCAGC TGTACAGGCT GGGCCGCCGA AGGAGAACCA TGAAACTCTG CAGAGAGCTC 
TCGGACTTGG TGGTGTACAC AAACTCCGTG GCAGCCCAGG ATATTGTTGA TGATGGAACC 
ACAGGGAATG TGTTGTCCTT CAGCGAAACA AGAGCCCATC AAGTGGTTCA GCAGAAGTCA 
GAGCAGTTCA TGATCTACAA CCAAAAACAG CTCACAAGGA TTTACCCCTC TGCCTACCGC 
ATCGATTCCA GTAACTTCAA CCCACTGCCC TACTGGAATG CAGGCTGCCA GCTCGTGGCT 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGATCAATC GAGCAAAATT CAAGGCGAAT 
GGCAACTGTG GCTATATTCT CAAGCCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGACC CACTTCCTGC CAACCCCAAA AAACAGCTCA TACTGAAAGT GATCAGTGGA 
CAGCAACTCC CCAAACCTCC TGACTCTATG TTTGGAGACC GAGGCGAGAT CATTGACCCT 
TTCGTTGAAG TTGAAATTAT TGGGCTGCCA GTAGACTGTT GCAAGGACCA AACTCGTGTG 
GTGGATGACA ACGGATTTAA CCCTGTGTGG GAAGAAACCC TAACTTTTAC AGTTCACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCTATTGG AAGAGACTTT 
GTCGGACAAA GGACTGTGAC CTTCAGTAGC CTCGTGCCCG GCTACCGGCA TGTCTACCTG 
GAAGGACTCA CAGAAGCATC TATTTTCGTT CACATAACCA TCAATGAGAT CTTTGGAAAG 
TGGAGTCCTT TAATCCTCAA CCCCAGTTAT ACCATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGCCT GAAGGGGCTG TTCAATAAGA ATCCCAGGCA CGCTTCTTCA 
GAAAACAACT CGCATTATGT TCGGAAGCGA TCGATTGGAG ATAGGATTCT GCGACGTACA 
GCTAGTGCTC CAGCCAAAGG CAGGAAAAAG AGCAAAGTGG GCTTCCAAGA AATGGTTGAG 
ATAAAAGATT CTGTCTCTGA GGCTTCAAGA GATCAAGATG GTGTCCTGAG GAGGACCACC 
CGGAGTCTGC AAGTACGCCC TGTCTCTATG CCTGTTGACA AGAGCCTTCT GGGGGCTTTG 
TCGCTGCCCA TATCTGAAGC AGCAAAAGAC ACGGATGGAA AAGAAAACTG TCTGGCAGGA 
GACAAGGATG ACAGAAGAAA GGGAGCCGCA ACTAGAAAAG ACCCACATTT TTCAAATTTC 
AACAAAAAGT TATCCTCCTC CTCCAGTGCG CTCCTCCACA AAGATGCCAA CCAAGGGCCA 
ACTGCCAGTG TATCAAACCC AGAACAGTGT GGAGGACGAG GTGCAAAGAG TGAGAGGATC 
AAACCAAATA TGACAAATGA TTGCCAGGAA AACCACAATC CCCCGAAATT CCTTTCTCCA 
AGGAAGCATT TGGCTCTGGA TCCTGCAACG AAGGGACTGC AAGAGAGACT GCATGGAATG 
AAAACCAATG AGAAGGAGCA TGCCGAGGGC TTCTTGGGAG AGAAAAGCAT GCTGTCCGGA 
AGCGTTCTTT CTCAAAGTTC TCTGGAGGTA GAGAACCTGG AAGGCAGCAG AGCCAAGGGC 
AGAGCTGCAA CCTCCTTTTC TCTCTCGGAC GTCTCCGCGC TCTGTTCTGA CATCCCTGAT 
TTACATTCAA CTGCCATTCT ACAGGACACT GAAATTTCCA ATCTCATTGA TGACGTGACT 
TTAACAAATG AGAATCAGTC GGGAAGTTCC ATCTCGGCAC TGATTGGCCA GTTTGAAGAA 
AGCAACCACC CGGCTAACGT TACTGTGGTT TCTCATCTCA GCACCAGTGG GGCATCAGGA 
AGTGCTCCTT TCCAAACACC CTTCAAGCAT GGTCTTTCCC AGGGGAACCA GAAGGCCTCC 
TTCTTGTGCT CATCTCCTGA GCTGAATAAG CTCTCTAGTG TTGAGACCAC CAAACTGGCA 
AATAACGCAG TTCCTTGTGG AGTGATTGGT TCTCCCATCT CTACACCAAA GCCAGGTGAT 
GACCCTTCAG ATAAGGCCAA GACAAGAGTC ATTGAAGGCA ACCTGCCTGG GTTCCCTGAT 
GCTTCTCCTG GTCAATTTCC CAAAAGCCCC ACCCATGGAG AGGACCATAG TCAAGTGATG 
AACAGCCCTG CTCTCTCCAC GGAGTTGGCC ATAGAAGATA TAATTGCAGA CCCTGCTCTC 
TCTATAAATT CTGCAGAAAG CAGTCTTGTG GAAATTGATG GAGAATCTGA AAACCTATCT 
CTAACAACCT GTGACTACAG GGAAGAAGCT CCAAGTCAAC TTGTTTCTCC ACTAAAACTG 
CAGCAGAGCC AGGAGATGGT GGAACACATT CAGAGGGGCT TGAGGAATGG CTACTGTAAA 
GAGACTCTCC TCCCTTCTGA AATATTCAAC AATATTCCAG GTGTCAAAAA TCACAGTATT 
TCTCATCTAA CCTATCAGGG TGCTGGCTTT GTGTATAACC ATTTCTCAAG TTCAGATGCA 
AAAACGAACC AAATCTGTGA GCCCCAGCAG CCTAGGGCTC CAGATATGCA TGCCCCTACA 
CCTACACCAT CAACACATGC TCCTTTGGCT GCTTTGAAAC TGCCAAGCCC ATGCAAATCC 
AAAAGTCTGG GGGACTTAAC ATCAGAGGAC ATTGCCTGCA ATTTTGAAAG CAAATATCAA 
TGTATTAGTA GGAGCTTTGT GACAAACGGC ATTAGGGACA AGAGCGTGAC TATGAAGACA 
AAGTCATTAG AGCCTTTAGA CGCCCTGACT GAACAGCTCC GGAAGCTGGT GTCCTTTGAC 
CAAGAAGACA GCTGTCAAGT GCTCTACTCA AAGCAGGATG TCAATCAGTG TCCCAGGGCA 
TTAGTCAGAA AGTTGTCGTC TAGAAGTCAG AGCAGAGTGC GCAACATTGC TAGCCGTGCC 
AAGGAGAAGC AGGAAGCTGG CAAGCAAAAA GCTATGGCCC AGAGCACCAG AGGAGGAGTG 
GTCCTTAGAA GTAAACCGCC CGCTCCTGCT CTGGCCGTGA ACCGTCACTC CACGGGCTCA 
TACATTGCAA GCTACCTGAG GAACATGAAA GCTGGTGGCC TAGAGGGTCG AGGCATCCCA 
GAGGGAGCGT GCACGGCCCT TCGCTATGGC TACATGGACC AGTTTTGTTC AGATAATTCA 
GTTCTGCAGA CTGAGCCAAG CAGCGAAGAT AAACCCGAAA TTTATTTTCT TTTGAGGCTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319595.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175084.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu80626
Clone ID Related Accession (Same CDS sequence)	XM_017319595.2 , XM_017319595.1
Accession Version	XM_017319595.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AACCTGGATG TAAAGGAAAG TGGAAAGAAG 
TCCCATGGGC GATCCCTGAT GGCCAACTTT GGGAAACACA AGCAGAAAGC TACGAAATCA 
CGTTCTAAAT CCTATAGTAC TGATGATGAG GATGATAGCC TTCAGAATCC TGGCAAGGAG 
GGAGGCCAGC TGTACAGGCT GGGCCGCCGA AGGAGAACCA TGAAACTCTG CAGAGAGCTC 
TCGGACTTGG TGGTGTACAC AAACTCCGTG GCAGCCCAGG ATATTGTTGA TGATGGAACC 
ACAGGGAATG TGTTGTCCTT CAGCGAAACA AGAGCCCATC AAGTGGTTCA GCAGAAGTCA 
GAGCAGTTCA TGATCTACAA CCAAAAACAG CTCACAAGGA TTTACCCCTC TGCCTACCGC 
ATCGATTCCA GTAACTTCAA CCCACTGCCC TACTGGAATG CAGGCTGCCA GCTCGTGGCT 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGATCAATC GAGCAAAATT CAAGGCGAAT 
GGCAACTGTG GCTATATTCT CAAGCCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGACC CACTTCCTGC CAACCCCAAA AAACAGCTCA TACTGAAAGT GATCAGTGGA 
CAGCAACTCC CCAAACCTCC TGACTCTATG TTTGGAGACC GAGGCGAGAT CATTGACCCT 
TTCGTTGAAG TTGAAATTAT TGGGCTGCCA GTAGACTGTT GCAAGGACCA AACTCGTGTG 
GTGGATGACA ACGGATTTAA CCCTGTGTGG GAAGAAACCC TAACTTTTAC AGTTCACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCTATTGG AAGAGACTTT 
GTCGGACAAA GGACTGTGAC CTTCAGTAGC CTCGTGCCCG GCTACCGGCA TGTCTACCTG 
GAAGGACTCA CAGAAGCATC TATTTTCGTT CACATAACCA TCAATGAGAT CTTTGGAAAG 
TGGAGTCCTT TAATCCTCAA CCCCAGTTAT ACCATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGCCT GAAGGGGCTG TTCAATAAGA ATCCCAGGCA CGCTTCTTCA 
GAAAACAACT CGCATTATGT TCGGAAGCGA TCGATTGGAG ATAGGATTCT GCGACGTACA 
GCTAGTGCTC CAGCCAAAGG CAGGAAAAAG AGCAAAGTGG GCTTCCAAGA AATGGTTGAG 
ATAAAAGATT CTGTCTCTGA GGCTTCAAGA GATCAAGATG GTGTCCTGAG GAGGACCACC 
CGGAGTCTGC AAGTACGCCC TGTCTCTATG CCTGTTGACA AGAGCCTTCT GGGGGCTTTG 
TCGCTGCCCA TATCTGAAGC AGCAAAAGAC ACGGATGGAA AAGAAAACTG TCTGGCAGGA 
GACAAGGATG ACAGAAGAAA GGGAGCCGCA ACTAGAAAAG ACCCACATTT TTCAAATTTC 
AACAAAAAGT TATCCTCCTC CTCCAGTGCG CTCCTCCACA AAGATGCCAA CCAAGGGCCA 
ACTGCCAGTG TATCAAACCC AGAACAGTGT GGAGGACGAG GTGCAAAGAG TGAGAGGATC 
AAACCAAATA TGACAAATGA TTGCCAGGAA AACCACAATC CCCCGAAATT CCTTTCTCCA 
AGGAAGCATT TGGCTCTGGA TCCTGCAACG AAGGGACTGC AAGAGAGACT GCATGGAATG 
AAAACCAATG AGAAGGAGCA TGCCGAGGGC TTCTTGGGAG AGAAAAGCAT GCTGTCCGGA 
AGCGTTCTTT CTCAAAGTTC TCTGGAGGTA GAGAACCTGG AAGGCAGCAG AGCCAAGGGC 
AGAGCTGCAA CCTCCTTTTC TCTCTCGGAC GTCTCCGCGC TCTGTTCTGA CATCCCTGAT 
TTACATTCAA CTGCCATTCT ACAGGACACT GAAATTTCCA ATCTCATTGA TGACGTGACT 
TTAACAAATG AGAATCAGTC GGGAAGTTCC ATCTCGGCAC TGATTGGCCA GTTTGAAGAA 
AGCAACCACC CGGCTAACGT TACTGTGGTT TCTCATCTCA GCACCAGTGG GGCATCAGGA 
AGTGCTCCTT TCCAAACACC CTTCAAGCAT GGTCTTTCCC AGGGGAACCA GAAGGCCTCC 
TTCTTGTGCT CATCTCCTGA GCTGAATAAG CTCTCTAGTG TTGAGACCAC CAAACTGGCA 
AATAACGCAG TTCCTTGTGG AGTGATTGGT TCTCCCATCT CTACACCAAA GCCAGGTGAT 
GACCCTTCAG ATAAGGCCAA GACAAGAGTC ATTGAAGGCA ACCTGCCTGG GTTCCCTGAT 
GCTTCTCCTG GTCAATTTCC CAAAAGCCCC ACCCATGGAG AGGACCATAG TCAAGTGATG 
AACAGCCCTG CTCTCTCCAC GGAGTTGGCC ATAGAAGATA TAATTGCAGA CCCTGCTCTC 
TCTATAAATT CTGCAGAAAG CAGTCTTGTG GAAATTGATG GAGAATCTGA AAACCTATCT 
CTAACAACCT GTGACTACAG GGAAGAAGCT CCAAGTCAAC TTGTTTCTCC ACTAAAACTG 
CAGCAGAGCC AGGAGATGGT GGAACACATT CAGAGGGGCT TGAGGAATGG CTACTGTAAA 
GAGACTCTCC TCCCTTCTGA AATATTCAAC AATATTCCAG GTGTCAAAAA TCACAGTATT 
TCTCATCTAA CCTATCAGGG TGCTGGCTTT GTGTATAACC ATTTCTCAAG TTCAGATGCA 
AAAACGAACC AAATCTGTGA GCCCCAGCAG CCTAGGGCTC CAGATATGCA TGCCCCTACA 
CCTACACCAT CAACACATGC TCCTTTGGCT GCTTTGAAAC TGCCAAGCCC ATGCAAATCC 
AAAAGTCTGG GGGACTTAAC ATCAGAGGAC ATTGCCTGCA ATTTTGAAAG CAAATATCAA 
TGTATTAGTA GGAGCTTTGT GACAAACGGC ATTAGGGACA AGAGCGTGAC TATGAAGACA 
AAGTCATTAG AGCCTTTAGA CGCCCTGACT GAACAGCTCC GGAAGCTGGT GTCCTTTGAC 
CAAGAAGACA GCTGTCAAGT GCTCTACTCA AAGCAGGATG TCAATCAGTG TCCCAGGGCA 
TTAGTCAGAA AGTTGTCGTC TAGAAGTCAG AGCAGAGTGC GCAACATTGC TAGCCGTGCC 
AAGGAGAAGC AGGAAGCTGG CAAGCAAAAA GCTATGGCCC AGAGCACCAG AGGAGGAGTG 
GTCCTTAGAA GTAAACCGCC CGCTCCTGCT CTGGCCGTGA ACCGTCACTC CACGGGCTCA 
TACATTGCAA GCTACCTGAG GAACATGAAA GCTGGTGGCC TAGAGGGTCG AGGCATCCCA 
GAGGGAGCGT GCACGGCCCT TCGCTATGGC TACATGGACC AGTTTTGTTC AGATAATTCA 
GTTCTGCAGA CTGAGCCAAG CAGCGAAGAT AAACCCGAAA TTTATTTTCT TTTGAGGCTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175084.1
CDS	587..5509
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_017319595.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319595.1

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AACCTGGATG TAAAGGAAAG TGGAAAGAAG 
TCCCATGGGC GATCCCTGAT GGCCAACTTT GGGAAACACA AGCAGAAAGC TACGAAATCA 
CGTTCTAAAT CCTATAGTAC TGATGATGAG GATGATAGCC TTCAGAATCC TGGCAAGGAG 
GGAGGCCAGC TGTACAGGCT GGGCCGCCGA AGGAGAACCA TGAAACTCTG CAGAGAGCTC 
TCGGACTTGG TGGTGTACAC AAACTCCGTG GCAGCCCAGG ATATTGTTGA TGATGGAACC 
ACAGGGAATG TGTTGTCCTT CAGCGAAACA AGAGCCCATC AAGTGGTTCA GCAGAAGTCA 
GAGCAGTTCA TGATCTACAA CCAAAAACAG CTCACAAGGA TTTACCCCTC TGCCTACCGC 
ATCGATTCCA GTAACTTCAA CCCACTGCCC TACTGGAATG CAGGCTGCCA GCTCGTGGCT 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGATCAATC GAGCAAAATT CAAGGCGAAT 
GGCAACTGTG GCTATATTCT CAAGCCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGACC CACTTCCTGC CAACCCCAAA AAACAGCTCA TACTGAAAGT GATCAGTGGA 
CAGCAACTCC CCAAACCTCC TGACTCTATG TTTGGAGACC GAGGCGAGAT CATTGACCCT 
TTCGTTGAAG TTGAAATTAT TGGGCTGCCA GTAGACTGTT GCAAGGACCA AACTCGTGTG 
GTGGATGACA ACGGATTTAA CCCTGTGTGG GAAGAAACCC TAACTTTTAC AGTTCACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCTATTGG AAGAGACTTT 
GTCGGACAAA GGACTGTGAC CTTCAGTAGC CTCGTGCCCG GCTACCGGCA TGTCTACCTG 
GAAGGACTCA CAGAAGCATC TATTTTCGTT CACATAACCA TCAATGAGAT CTTTGGAAAG 
TGGAGTCCTT TAATCCTCAA CCCCAGTTAT ACCATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGCCT GAAGGGGCTG TTCAATAAGA ATCCCAGGCA CGCTTCTTCA 
GAAAACAACT CGCATTATGT TCGGAAGCGA TCGATTGGAG ATAGGATTCT GCGACGTACA 
GCTAGTGCTC CAGCCAAAGG CAGGAAAAAG AGCAAAGTGG GCTTCCAAGA AATGGTTGAG 
ATAAAAGATT CTGTCTCTGA GGCTTCAAGA GATCAAGATG GTGTCCTGAG GAGGACCACC 
CGGAGTCTGC AAGTACGCCC TGTCTCTATG CCTGTTGACA AGAGCCTTCT GGGGGCTTTG 
TCGCTGCCCA TATCTGAAGC AGCAAAAGAC ACGGATGGAA AAGAAAACTG TCTGGCAGGA 
GACAAGGATG ACAGAAGAAA GGGAGCCGCA ACTAGAAAAG ACCCACATTT TTCAAATTTC 
AACAAAAAGT TATCCTCCTC CTCCAGTGCG CTCCTCCACA AAGATGCCAA CCAAGGGCCA 
ACTGCCAGTG TATCAAACCC AGAACAGTGT GGAGGACGAG GTGCAAAGAG TGAGAGGATC 
AAACCAAATA TGACAAATGA TTGCCAGGAA AACCACAATC CCCCGAAATT CCTTTCTCCA 
AGGAAGCATT TGGCTCTGGA TCCTGCAACG AAGGGACTGC AAGAGAGACT GCATGGAATG 
AAAACCAATG AGAAGGAGCA TGCCGAGGGC TTCTTGGGAG AGAAAAGCAT GCTGTCCGGA 
AGCGTTCTTT CTCAAAGTTC TCTGGAGGTA GAGAACCTGG AAGGCAGCAG AGCCAAGGGC 
AGAGCTGCAA CCTCCTTTTC TCTCTCGGAC GTCTCCGCGC TCTGTTCTGA CATCCCTGAT 
TTACATTCAA CTGCCATTCT ACAGGACACT GAAATTTCCA ATCTCATTGA TGACGTGACT 
TTAACAAATG AGAATCAGTC GGGAAGTTCC ATCTCGGCAC TGATTGGCCA GTTTGAAGAA 
AGCAACCACC CGGCTAACGT TACTGTGGTT TCTCATCTCA GCACCAGTGG GGCATCAGGA 
AGTGCTCCTT TCCAAACACC CTTCAAGCAT GGTCTTTCCC AGGGGAACCA GAAGGCCTCC 
TTCTTGTGCT CATCTCCTGA GCTGAATAAG CTCTCTAGTG TTGAGACCAC CAAACTGGCA 
AATAACGCAG TTCCTTGTGG AGTGATTGGT TCTCCCATCT CTACACCAAA GCCAGGTGAT 
GACCCTTCAG ATAAGGCCAA GACAAGAGTC ATTGAAGGCA ACCTGCCTGG GTTCCCTGAT 
GCTTCTCCTG GTCAATTTCC CAAAAGCCCC ACCCATGGAG AGGACCATAG TCAAGTGATG 
AACAGCCCTG CTCTCTCCAC GGAGTTGGCC ATAGAAGATA TAATTGCAGA CCCTGCTCTC 
TCTATAAATT CTGCAGAAAG CAGTCTTGTG GAAATTGATG GAGAATCTGA AAACCTATCT 
CTAACAACCT GTGACTACAG GGAAGAAGCT CCAAGTCAAC TTGTTTCTCC ACTAAAACTG 
CAGCAGAGCC AGGAGATGGT GGAACACATT CAGAGGGGCT TGAGGAATGG CTACTGTAAA 
GAGACTCTCC TCCCTTCTGA AATATTCAAC AATATTCCAG GTGTCAAAAA TCACAGTATT 
TCTCATCTAA CCTATCAGGG TGCTGGCTTT GTGTATAACC ATTTCTCAAG TTCAGATGCA 
AAAACGAACC AAATCTGTGA GCCCCAGCAG CCTAGGGCTC CAGATATGCA TGCCCCTACA 
CCTACACCAT CAACACATGC TCCTTTGGCT GCTTTGAAAC TGCCAAGCCC ATGCAAATCC 
AAAAGTCTGG GGGACTTAAC ATCAGAGGAC ATTGCCTGCA ATTTTGAAAG CAAATATCAA 
TGTATTAGTA GGAGCTTTGT GACAAACGGC ATTAGGGACA AGAGCGTGAC TATGAAGACA 
AAGTCATTAG AGCCTTTAGA CGCCCTGACT GAACAGCTCC GGAAGCTGGT GTCCTTTGAC 
CAAGAAGACA GCTGTCAAGT GCTCTACTCA AAGCAGGATG TCAATCAGTG TCCCAGGGCA 
TTAGTCAGAA AGTTGTCGTC TAGAAGTCAG AGCAGAGTGC GCAACATTGC TAGCCGTGCC 
AAGGAGAAGC AGGAAGCTGG CAAGCAAAAA GCTATGGCCC AGAGCACCAG AGGAGGAGTG 
GTCCTTAGAA GTAAACCGCC CGCTCCTGCT CTGGCCGTGA ACCGTCACTC CACGGGCTCA 
TACATTGCAA GCTACCTGAG GAACATGAAA GCTGGTGGCC TAGAGGGTCG AGGCATCCCA 
GAGGGAGCGT GCACGGCCCT TCGCTATGGC TACATGGACC AGTTTTGTTC AGATAATTCA 
GTTCTGCAGA CTGAGCCAAG CAGCGAAGAT AAACCCGAAA TTTATTTTCT TTTGAGGCTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_030252632.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108492.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu88232
Clone ID Related Accession (Same CDS sequence)	XM_030252632.1
Accession Version	XM_030252632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGGAGACA AGGATGACAG AAGAAAGGGA 
GCCGCAACTA GAAAAGACCC ACATTTTTCA AATTTCAACA AAAAGTTATC CTCCTCCTCC 
AGTGCGCTCC TCCACAAAGA TGCCAACCAA GGGCCAACTG CCAGTGTATC AAACCCAGAA 
CAGTGTGGAG GACGAGGTGC AAAGAGTGAG AGGATCAAAC CAAATATGAC AAATGATTGC 
CAGGAAAACC ACAATCCCCC GAAATTCCTT TCTCCAAGGA AGCATTTGGC TCTGGATCCT 
GCAACGAAGG GACTGCAAGA GAGACTGCAT GGAATGAAAA CCAATGAGAA GGAGCATGCC 
GAGGGCTTCT TGGGAGAGAA AAGCATGCTG TCCGGAAGCG TTCTTTCTCA AAGTTCTCTG 
GAGGTAGAGA ACCTGGAAGG CAGCAGAGCC AAGGGCAGAG CTGCAACCTC CTTTTCTCTC 
TCGGACGTCT CCGCGCTCTG TTCTGACATC CCTGATTTAC ATTCAACTGC CATTCTACAG 
GACACTGAAA TTTCCAATCT CATTGATGAC GTGACTTTAA CAAATGAGAA TCAGTCGGGA 
AGTTCCATCT CGGCACTGAT TGGCCAGTTT GAAGAAAGCA ACCACCCGGC TAACGTTACT 
GTGGTTTCTC ATCTCAGCAC CAGTGGGGCA TCAGGAAGTG CTCCTTTCCA AACACCCTTC 
AAGCATGGTC TTTCCCAGGG GAACCAGAAG GCCTCCTTCT TGTGCTCATC TCCTGAGCTG 
AATAAGCTCT CTAGTGTTGA GACCACCAAA CTGGCAAATA ACGCAGTTCC TTGTGGAGTG 
ATTGGTTCTC CCATCTCTAC ACCAAAGCCA GGTGATGACC CTTCAGATAA GGCCAAGACA 
AGAGTCATTG AAGGCAACCT GCCTGGGTTC CCTGATGCTT CTCCTGGTCA ATTTCCCAAA 
AGCCCCACCC ATGGAGAGGA CCATAGTCAA GTGATGAACA GCCCTGCTCT CTCCACGGAG 
TTGGCCATAG AAGATATAAT TGCAGACCCT GCTCTCTCTA TAAATTCTGC AGAAAGCAGT 
CTTGTGGAAA TTGATGGAGA ATCTGAAAAC CTATCTCTAA CAACCTGTGA CTACAGGGAA 
GAAGCTCCAA GTCAACTTGT TTCTCCACTA AAACTGCAGC AGAGCCAGGA GATGGTGGAA 
CACATTCAGA GGGGCTTGAG GAATGGCTAC TGTAAAGAGA CTCTCCTCCC TTCTGAAATA 
TTCAACAATA TTCCAGGTGT CAAAAATCAC AGTATTTCTC ATCTAACCTA TCAGGGTGCT 
GGCTTTGTGT ATAACCATTT CTCAAGTTCA GATGCAAAAA CGAACCAAAT CTGTGAGCCC 
CAGCAGCCTA GGGCTCCAGA TATGCATGCC CCTACACCTA CACCATCAAC ACATGCTCCT 
TTGGCTGCTT TGAAACTGCC AAGCCCATGC AAATCCAAAA GTCTGGGGGA CTTAACATCA 
GAGGACATTG CCTGCAATTT TGAAAGCAAA TATCAATGTA TTAGTAGGAG CTTTGTGACA 
AACGGCATTA GGGACAAGAG CGTGACTATG AAGACAAAGT CATTAGAGCC TTTAGACGCC 
CTGACTGAAC AGCTCCGGAA GCTGGTGTCC TTTGACCAAG AAGACAGCTG TCAAGTGCTC 
TACTCAAAGC AGGATGTCAA TCAGTGTCCC AGGGCATTAG TCAGAAAGTT GTCGTCTAGA 
AGTCAGAGCA GAGTGCGCAA CATTGCTAGC CGTGCCAAGG AGAAGCAGGA AGCTGGCAAG 
CAAAAAGCTA TGGCCCAGAG CACCAGAGGA GGAGTGGTCC TTAGAAGTAA ACCGCCCGCT 
CCTGCTCTGG CCGTGAACCG TCACTCCACG GGCTCATACA TTGCAAGCTA CCTGAGGAAC 
ATGAAAGCTG GTGGCCTAGA GGGTCGAGGC ATCCCAGAGG GAGCGTGCAC GGCCCTTCGC 
TATGGCTACA TGGACCAGTT TTGTTCAGAT AATTCAGTTC TGCAGACTGA GCCAAGCAGC 
GAAGATAAAC CCGAAATTTA TTTTCTTTTG AGGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108492.1
CDS	402..5420
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_030252632.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252632.1

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGGAGACA AGGATGACAG AAGAAAGGGA 
GCCGCAACTA GAAAAGACCC ACATTTTTCA AATTTCAACA AAAAGTTATC CTCCTCCTCC 
AGTGCGCTCC TCCACAAAGA TGCCAACCAA GGGCCAACTG CCAGTGTATC AAACCCAGAA 
CAGTGTGGAG GACGAGGTGC AAAGAGTGAG AGGATCAAAC CAAATATGAC AAATGATTGC 
CAGGAAAACC ACAATCCCCC GAAATTCCTT TCTCCAAGGA AGCATTTGGC TCTGGATCCT 
GCAACGAAGG GACTGCAAGA GAGACTGCAT GGAATGAAAA CCAATGAGAA GGAGCATGCC 
GAGGGCTTCT TGGGAGAGAA AAGCATGCTG TCCGGAAGCG TTCTTTCTCA AAGTTCTCTG 
GAGGTAGAGA ACCTGGAAGG CAGCAGAGCC AAGGGCAGAG CTGCAACCTC CTTTTCTCTC 
TCGGACGTCT CCGCGCTCTG TTCTGACATC CCTGATTTAC ATTCAACTGC CATTCTACAG 
GACACTGAAA TTTCCAATCT CATTGATGAC GTGACTTTAA CAAATGAGAA TCAGTCGGGA 
AGTTCCATCT CGGCACTGAT TGGCCAGTTT GAAGAAAGCA ACCACCCGGC TAACGTTACT 
GTGGTTTCTC ATCTCAGCAC CAGTGGGGCA TCAGGAAGTG CTCCTTTCCA AACACCCTTC 
AAGCATGGTC TTTCCCAGGG GAACCAGAAG GCCTCCTTCT TGTGCTCATC TCCTGAGCTG 
AATAAGCTCT CTAGTGTTGA GACCACCAAA CTGGCAAATA ACGCAGTTCC TTGTGGAGTG 
ATTGGTTCTC CCATCTCTAC ACCAAAGCCA GGTGATGACC CTTCAGATAA GGCCAAGACA 
AGAGTCATTG AAGGCAACCT GCCTGGGTTC CCTGATGCTT CTCCTGGTCA ATTTCCCAAA 
AGCCCCACCC ATGGAGAGGA CCATAGTCAA GTGATGAACA GCCCTGCTCT CTCCACGGAG 
TTGGCCATAG AAGATATAAT TGCAGACCCT GCTCTCTCTA TAAATTCTGC AGAAAGCAGT 
CTTGTGGAAA TTGATGGAGA ATCTGAAAAC CTATCTCTAA CAACCTGTGA CTACAGGGAA 
GAAGCTCCAA GTCAACTTGT TTCTCCACTA AAACTGCAGC AGAGCCAGGA GATGGTGGAA 
CACATTCAGA GGGGCTTGAG GAATGGCTAC TGTAAAGAGA CTCTCCTCCC TTCTGAAATA 
TTCAACAATA TTCCAGGTGT CAAAAATCAC AGTATTTCTC ATCTAACCTA TCAGGGTGCT 
GGCTTTGTGT ATAACCATTT CTCAAGTTCA GATGCAAAAA CGAACCAAAT CTGTGAGCCC 
CAGCAGCCTA GGGCTCCAGA TATGCATGCC CCTACACCTA CACCATCAAC ACATGCTCCT 
TTGGCTGCTT TGAAACTGCC AAGCCCATGC AAATCCAAAA GTCTGGGGGA CTTAACATCA 
GAGGACATTG CCTGCAATTT TGAAAGCAAA TATCAATGTA TTAGTAGGAG CTTTGTGACA 
AACGGCATTA GGGACAAGAG CGTGACTATG AAGACAAAGT CATTAGAGCC TTTAGACGCC 
CTGACTGAAC AGCTCCGGAA GCTGGTGTCC TTTGACCAAG AAGACAGCTG TCAAGTGCTC 
TACTCAAAGC AGGATGTCAA TCAGTGTCCC AGGGCATTAG TCAGAAAGTT GTCGTCTAGA 
AGTCAGAGCA GAGTGCGCAA CATTGCTAGC CGTGCCAAGG AGAAGCAGGA AGCTGGCAAG 
CAAAAAGCTA TGGCCCAGAG CACCAGAGGA GGAGTGGTCC TTAGAAGTAA ACCGCCCGCT 
CCTGCTCTGG CCGTGAACCG TCACTCCACG GGCTCATACA TTGCAAGCTA CCTGAGGAAC 
ATGAAAGCTG GTGGCCTAGA GGGTCGAGGC ATCCCAGAGG GAGCGTGCAC GGCCCTTCGC 
TATGGCTACA TGGACCAGTT TTGTTCAGAT AATTCAGTTC TGCAGACTGA GCCAAGCAGC 
GAAGATAAAC CCGAAATTTA TTTTCTTTTG AGGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( NM_183191.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_899014.2 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 1, mRNA.

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CloneID	OMu15698
Clone ID Related Accession (Same CDS sequence)	NM_183191.3 , NM_183191.4
Accession Version	NM_183191.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY691174.1 and BQ177171.1. On Apr 25, 2010 this sequence version replaced NM_183191.2. Transcript Variant: This variant (1, also known as PLC-eta1c) encodes the longest isoform (1, also known as phospholipase C-eta1c). CCDS Note: The coding region has been updated to represent an alternate splice pattern that is more supported by the available conservation, transcript and protein data. The update changes the protein length from 1640 aa to 1682 aa. ##Evidence-Data-START## Transcript exon combination :: AY691174.1, SRR1660817.306304.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGCA GAAAGCTACG 
AAATCACGTT CTAAATCCTA TAGTACTGAT GATGAGGATG ATAGCCTTCA GAATCCTGGC 
AAGGAGGGAG GCCAGCTGTA CAGGCTGGGC CGCCGAAGGA GAACCATGAA ACTCTGCAGA 
GAGCTCTCGG ACTTGGTGGT GTACACAAAC TCCGTGGCAG CCCAGGATAT TGTTGATGAT 
GGAACCACAG GGAATGTGTT GTCCTTCAGC GAAACAAGAG CCCATCAAGT GGTTCAGCAG 
AAGTCAGAGC AGTTCATGAT CTACAACCAA AAACAGCTCA CAAGGATTTA CCCCTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCA CTGCCCTACT GGAATGCAGG CTGCCAGCTC 
GTGGCTCTGA ACTACCAGTC TGAAGGGCGA ATGATGCAGA TCAATCGAGC AAAATTCAAG 
GCGAATGGCA ACTGTGGCTA TATTCTCAAG CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTTCTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAAC AGCTCATACT GAAAGTGATC 
AGTGGACAGC AACTCCCCAA ACCTCCTGAC TCTATGTTTG GAGACCGAGG CGAGATCATT 
GACCCTTTCG TTGAAGTTGA AATTATTGGG CTGCCAGTAG ACTGTTGCAA GGACCAAACT 
CGTGTGGTGG ATGACAACGG ATTTAACCCT GTGTGGGAAG AAACCCTAAC TTTTACAGTT 
CACATGCCAG AAATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC TATTGGAAGA 
GACTTTGTCG GACAAAGGAC TGTGACCTTC AGTAGCCTCG TGCCCGGCTA CCGGCATGTC 
TACCTGGAAG GACTCACAGA AGCATCTATT TTCGTTCACA TAACCATCAA TGAGATCTTT 
GGAAAGTGGA GTCCTTTAAT CCTCAACCCC AGTTATACCA TATTGCACTT TCTAGGAGCT 
ACAAAGAACA GACAGCTCCA AGGCCTGAAG GGGCTGTTCA ATAAGAATCC CAGGCACGCT 
TCTTCAGAAA ACAACTCGCA TTATGTTCGG AAGCGATCGA TTGGAGATAG GATTCTGCGA 
CGTACAGCTA GTGCTCCAGC CAAAGGCAGG AAAAAGAGCA AAGTGGGCTT CCAAGAAATG 
GTTGAGATAA AAGATTCTGT CTCTGAGGCT TCAAGAGATC AAGATGGTGT CCTGAGGAGG 
ACCACCCGGA GTCTGCAAGT ACGCCCTGTC TCTATGCCTG TTGACAAGAG CCTTCTGGGG 
GCTTTGTCGC TGCCCATATC TGAAGCAGCA AAAGACACGG ATGGAAAAGA AAACTGTCTG 
GCAGGAGACA AGGATGACAG AAGAAAGGGA GCCGCAACTA GAAAAGACCC ACATTTTTCA 
AATTTCAACA AAAAGTTATC CTCCTCCTCC AGTGCGCTCC TCCACAAAGA TGCCAACCAA 
GGGCCAACTG CCAGTGTATC AAACCCAGAA CAGTGTGGAG GACGAGGTGC AAAGAGTGAG 
AGGATCAAAC CAAATATGAC AAATGATTGC CAGGAAAACC ACAATCCCCC GAAATTCCTT 
TCTCCAAGGA AGCATTTGGC TCTGGATCCT GCAACGAAGG GACTGCAAGA GAGACTGCAT 
GGAATGAAAA CCAATGAGAA GGAGCATGCC GAGGGCTTCT TGGGAGAGAA AAGCATGCTG 
TCCGGAAGCG TTCTTTCTCA AAGTTCTCTG GAGGTAGAGA ACCTGGAAGG CAGCAGAGCC 
AAGGGCAGAG CTGCAACCTC CTTTTCTCTC TCGGACGTCT CCGCGCTCTG TTCTGACATC 
CCTGATTTAC ATTCAACTGC CATTCTACAG GACACTGAAA TTTCCAATCT CATTGATGAC 
GTGACTTTAA CAAATGAGAA TCAGTCGGGA AGTTCCATCT CGGCACTGAT TGGCCAGTTT 
GAAGAAAGCA ACCACCCGGC TAACGTTACT GTGGTTTCTC ATCTCAGCAC CAGTGGGGCA 
TCAGGAAGTG CTCCTTTCCA AACACCCTTC AAGCATGGTC TTTCCCAGGG GAACCAGAAG 
GCCTCCTTCT TGTGCTCATC TCCTGAGCTG AATAAGCTCT CTAGTGTTGA GACCACCAAA 
CTGGCAAATA ACGCAGTTCC TTGTGGAGTG ATTGGTTCTC CCATCTCTAC ACCAAAGCCA 
GGTGATGACC CTTCAGATAA GGCCAAGACA AGAGTCATTG AAGGCAACCT GCCTGGGTTC 
CCTGATGCTT CTCCTGGTCA ATTTCCCAAA AGCCCCACCC ATGGAGAGGA CCATAGTCAA 
GTGATGAACA GCCCTGCTCT CTCCACGGAG TTGGCCATAG AAGATATAAT TGCAGACCCT 
GCTCTCTCTA TAAATTCTGC AGAAAGCAGT CTTGTGGAAA TTGATGGAGA ATCTGAAAAC 
CTATCTCTAA CAACCTGTGA CTACAGGGAA GAAGCTCCAA GTCAACTTGT TTCTCCACTA 
AAACTGCAGC AGAGCCAGGA GATGGTGGAA CACATTCAGA GGGGCTTGAG GAATGGCTAC 
TGTAAAGAGA CTCTCCTCCC TTCTGAAATA TTCAACAATA TTCCAGGTGT CAAAAATCAC 
AGTATTTCTC ATCTAACCTA TCAGGGTGCT GGCTTTGTGT ATAACCATTT CTCAAGTTCA 
GATGCAAAAA CGAACCAAAT CTGTGAGCCC CAGCAGCCTA GGGCTCCAGA TATGCATGCC 
CCTACACCTA CACCATCAAC ACATGCTCCT TTGGCTGCTT TGAAACTGCC AAGCCCATGC 
AAATCCAAAA GTCTGGGGGA CTTAACATCA GAGGACATTG CCTGCAATTT TGAAAGCAAA 
TATCAATGTA TTAGTAGGAG CTTTGTGACA AACGGCATTA GGGACAAGAG CGTGACTATG 
AAGACAAAGT CATTAGAGCC TTTAGACGCC CTGACTGAAC AGCTCCGGAA GCTGGTGTCC 
TTTGACCAAG AAGACAGCTG TCAAGTGCTC TACTCAAAGC AGGATGTCAA TCAGTGTCCC 
AGGGCATTAG TCAGAAAGTT GTCGTCTAGA AGTCAGAGCA GAGTGCGCAA CATTGCTAGC 
CGTGCCAAGG AGAAGCAGGA AGCTGGCAAG CAAAAAGCTA TGGCCCAGAG CACCAGAGGA 
GGAGTGGTCC TTAGAAGTAA ACCGCCCGCT CCTGCTCTGG CCGTGAACCG TCACTCCACG 
GGCTCATACA TTGCAAGCTA CCTGAGGAAC ATGAAAGCTG GTGGCCTAGA GGGTCGAGGC 
ATCCCAGAGG GAGCGTGCAC GGCCCTTCGC TATGGCTACA TGGACCAGTT TTGTTCAGAT 
AATTCAGTTC TGCAGACTGA GCCAAGCAGC GAAGATAAAC CCGAAATTTA TTTTCTTTTG 
AGGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899014.2
CDS	1..5049
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	NM_183191.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183191.3

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGCA GAAAGCTACG 
AAATCACGTT CTAAATCCTA TAGTACTGAT GATGAGGATG ATAGCCTTCA GAATCCTGGC 
AAGGAGGGAG GCCAGCTGTA CAGGCTGGGC CGCCGAAGGA GAACCATGAA ACTCTGCAGA 
GAGCTCTCGG ACTTGGTGGT GTACACAAAC TCCGTGGCAG CCCAGGATAT TGTTGATGAT 
GGAACCACAG GGAATGTGTT GTCCTTCAGC GAAACAAGAG CCCATCAAGT GGTTCAGCAG 
AAGTCAGAGC AGTTCATGAT CTACAACCAA AAACAGCTCA CAAGGATTTA CCCCTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCA CTGCCCTACT GGAATGCAGG CTGCCAGCTC 
GTGGCTCTGA ACTACCAGTC TGAAGGGCGA ATGATGCAGA TCAATCGAGC AAAATTCAAG 
GCGAATGGCA ACTGTGGCTA TATTCTCAAG CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTTCTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAAC AGCTCATACT GAAAGTGATC 
AGTGGACAGC AACTCCCCAA ACCTCCTGAC TCTATGTTTG GAGACCGAGG CGAGATCATT 
GACCCTTTCG TTGAAGTTGA AATTATTGGG CTGCCAGTAG ACTGTTGCAA GGACCAAACT 
CGTGTGGTGG ATGACAACGG ATTTAACCCT GTGTGGGAAG AAACCCTAAC TTTTACAGTT 
CACATGCCAG AAATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC TATTGGAAGA 
GACTTTGTCG GACAAAGGAC TGTGACCTTC AGTAGCCTCG TGCCCGGCTA CCGGCATGTC 
TACCTGGAAG GACTCACAGA AGCATCTATT TTCGTTCACA TAACCATCAA TGAGATCTTT 
GGAAAGTGGA GTCCTTTAAT CCTCAACCCC AGTTATACCA TATTGCACTT TCTAGGAGCT 
ACAAAGAACA GACAGCTCCA AGGCCTGAAG GGGCTGTTCA ATAAGAATCC CAGGCACGCT 
TCTTCAGAAA ACAACTCGCA TTATGTTCGG AAGCGATCGA TTGGAGATAG GATTCTGCGA 
CGTACAGCTA GTGCTCCAGC CAAAGGCAGG AAAAAGAGCA AAGTGGGCTT CCAAGAAATG 
GTTGAGATAA AAGATTCTGT CTCTGAGGCT TCAAGAGATC AAGATGGTGT CCTGAGGAGG 
ACCACCCGGA GTCTGCAAGT ACGCCCTGTC TCTATGCCTG TTGACAAGAG CCTTCTGGGG 
GCTTTGTCGC TGCCCATATC TGAAGCAGCA AAAGACACGG ATGGAAAAGA AAACTGTCTG 
GCAGGAGACA AGGATGACAG AAGAAAGGGA GCCGCAACTA GAAAAGACCC ACATTTTTCA 
AATTTCAACA AAAAGTTATC CTCCTCCTCC AGTGCGCTCC TCCACAAAGA TGCCAACCAA 
GGGCCAACTG CCAGTGTATC AAACCCAGAA CAGTGTGGAG GACGAGGTGC AAAGAGTGAG 
AGGATCAAAC CAAATATGAC AAATGATTGC CAGGAAAACC ACAATCCCCC GAAATTCCTT 
TCTCCAAGGA AGCATTTGGC TCTGGATCCT GCAACGAAGG GACTGCAAGA GAGACTGCAT 
GGAATGAAAA CCAATGAGAA GGAGCATGCC GAGGGCTTCT TGGGAGAGAA AAGCATGCTG 
TCCGGAAGCG TTCTTTCTCA AAGTTCTCTG GAGGTAGAGA ACCTGGAAGG CAGCAGAGCC 
AAGGGCAGAG CTGCAACCTC CTTTTCTCTC TCGGACGTCT CCGCGCTCTG TTCTGACATC 
CCTGATTTAC ATTCAACTGC CATTCTACAG GACACTGAAA TTTCCAATCT CATTGATGAC 
GTGACTTTAA CAAATGAGAA TCAGTCGGGA AGTTCCATCT CGGCACTGAT TGGCCAGTTT 
GAAGAAAGCA ACCACCCGGC TAACGTTACT GTGGTTTCTC ATCTCAGCAC CAGTGGGGCA 
TCAGGAAGTG CTCCTTTCCA AACACCCTTC AAGCATGGTC TTTCCCAGGG GAACCAGAAG 
GCCTCCTTCT TGTGCTCATC TCCTGAGCTG AATAAGCTCT CTAGTGTTGA GACCACCAAA 
CTGGCAAATA ACGCAGTTCC TTGTGGAGTG ATTGGTTCTC CCATCTCTAC ACCAAAGCCA 
GGTGATGACC CTTCAGATAA GGCCAAGACA AGAGTCATTG AAGGCAACCT GCCTGGGTTC 
CCTGATGCTT CTCCTGGTCA ATTTCCCAAA AGCCCCACCC ATGGAGAGGA CCATAGTCAA 
GTGATGAACA GCCCTGCTCT CTCCACGGAG TTGGCCATAG AAGATATAAT TGCAGACCCT 
GCTCTCTCTA TAAATTCTGC AGAAAGCAGT CTTGTGGAAA TTGATGGAGA ATCTGAAAAC 
CTATCTCTAA CAACCTGTGA CTACAGGGAA GAAGCTCCAA GTCAACTTGT TTCTCCACTA 
AAACTGCAGC AGAGCCAGGA GATGGTGGAA CACATTCAGA GGGGCTTGAG GAATGGCTAC 
TGTAAAGAGA CTCTCCTCCC TTCTGAAATA TTCAACAATA TTCCAGGTGT CAAAAATCAC 
AGTATTTCTC ATCTAACCTA TCAGGGTGCT GGCTTTGTGT ATAACCATTT CTCAAGTTCA 
GATGCAAAAA CGAACCAAAT CTGTGAGCCC CAGCAGCCTA GGGCTCCAGA TATGCATGCC 
CCTACACCTA CACCATCAAC ACATGCTCCT TTGGCTGCTT TGAAACTGCC AAGCCCATGC 
AAATCCAAAA GTCTGGGGGA CTTAACATCA GAGGACATTG CCTGCAATTT TGAAAGCAAA 
TATCAATGTA TTAGTAGGAG CTTTGTGACA AACGGCATTA GGGACAAGAG CGTGACTATG 
AAGACAAAGT CATTAGAGCC TTTAGACGCC CTGACTGAAC AGCTCCGGAA GCTGGTGTCC 
TTTGACCAAG AAGACAGCTG TCAAGTGCTC TACTCAAAGC AGGATGTCAA TCAGTGTCCC 
AGGGCATTAG TCAGAAAGTT GTCGTCTAGA AGTCAGAGCA GAGTGCGCAA CATTGCTAGC 
CGTGCCAAGG AGAAGCAGGA AGCTGGCAAG CAAAAAGCTA TGGCCCAGAG CACCAGAGGA 
GGAGTGGTCC TTAGAAGTAA ACCGCCCGCT CCTGCTCTGG CCGTGAACCG TCACTCCACG 
GGCTCATACA TTGCAAGCTA CCTGAGGAAC ATGAAAGCTG GTGGCCTAGA GGGTCGAGGC 
ATCCCAGAGG GAGCGTGCAC GGCCCTTCGC TATGGCTACA TGGACCAGTT TTGTTCAGAT 
AATTCAGTTC TGCAGACTGA GCCAAGCAGC GAAGATAAAC CCGAAATTTA TTTTCTTTTG 
AGGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( NM_183191.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_899014.2 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 1, mRNA.

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CloneID	OMu15698
Clone ID Related Accession (Same CDS sequence)	NM_183191.3 , NM_183191.4
Accession Version	NM_183191.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-19
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116732.10 and AC121285.7. On Jun 20, 2019 this sequence version replaced NM_183191.3. Transcript Variant: This variant (1, also known as PLC-eta1c) encodes the longest isoform (1, also known as phospholipase C-eta1c). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: The coding region has been updated to represent an alternate splice pattern that is more supported by the available conservation, transcript and protein data. The update changes the protein length from 1640 aa to 1682 aa. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.306304.1, AY691174.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGCA GAAAGCTACG 
AAATCACGTT CTAAATCCTA TAGTACTGAT GATGAGGATG ATAGCCTTCA GAATCCTGGC 
AAGGAGGGAG GCCAGCTGTA CAGGCTGGGC CGCCGAAGGA GAACCATGAA ACTCTGCAGA 
GAGCTCTCGG ACTTGGTGGT GTACACAAAC TCCGTGGCAG CCCAGGATAT TGTTGATGAT 
GGAACCACAG GGAATGTGTT GTCCTTCAGC GAAACAAGAG CCCATCAAGT GGTTCAGCAG 
AAGTCAGAGC AGTTCATGAT CTACAACCAA AAACAGCTCA CAAGGATTTA CCCCTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCA CTGCCCTACT GGAATGCAGG CTGCCAGCTC 
GTGGCTCTGA ACTACCAGTC TGAAGGGCGA ATGATGCAGA TCAATCGAGC AAAATTCAAG 
GCGAATGGCA ACTGTGGCTA TATTCTCAAG CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTTCTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAAC AGCTCATACT GAAAGTGATC 
AGTGGACAGC AACTCCCCAA ACCTCCTGAC TCTATGTTTG GAGACCGAGG CGAGATCATT 
GACCCTTTCG TTGAAGTTGA AATTATTGGG CTGCCAGTAG ACTGTTGCAA GGACCAAACT 
CGTGTGGTGG ATGACAACGG ATTTAACCCT GTGTGGGAAG AAACCCTAAC TTTTACAGTT 
CACATGCCAG AAATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC TATTGGAAGA 
GACTTTGTCG GACAAAGGAC TGTGACCTTC AGTAGCCTCG TGCCCGGCTA CCGGCATGTC 
TACCTGGAAG GACTCACAGA AGCATCTATT TTCGTTCACA TAACCATCAA TGAGATCTTT 
GGAAAGTGGA GTCCTTTAAT CCTCAACCCC AGTTATACCA TATTGCACTT TCTAGGAGCT 
ACAAAGAACA GACAGCTCCA AGGCCTGAAG GGGCTGTTCA ATAAGAATCC CAGGCACGCT 
TCTTCAGAAA ACAACTCGCA TTATGTTCGG AAGCGATCGA TTGGAGATAG GATTCTGCGA 
CGTACAGCTA GTGCTCCAGC CAAAGGCAGG AAAAAGAGCA AAGTGGGCTT CCAAGAAATG 
GTTGAGATAA AAGATTCTGT CTCTGAGGCT TCAAGAGATC AAGATGGTGT CCTGAGGAGG 
ACCACCCGGA GTCTGCAAGT ACGCCCTGTC TCTATGCCTG TTGACAAGAG CCTTCTGGGG 
GCTTTGTCGC TGCCCATATC TGAAGCAGCA AAAGACACGG ATGGAAAAGA AAACTGTCTG 
GCAGGAGACA AGGATGACAG AAGAAAGGGA GCCGCAACTA GAAAAGACCC ACATTTTTCA 
AATTTCAACA AAAAGTTATC CTCCTCCTCC AGTGCGCTCC TCCACAAAGA TGCCAACCAA 
GGGCCAACTG CCAGTGTATC AAACCCAGAA CAGTGTGGAG GACGAGGTGC AAAGAGTGAG 
AGGATCAAAC CAAATATGAC AAATGATTGC CAGGAAAACC ACAATCCCCC GAAATTCCTT 
TCTCCAAGGA AGCATTTGGC TCTGGATCCT GCAACGAAGG GACTGCAAGA GAGACTGCAT 
GGAATGAAAA CCAATGAGAA GGAGCATGCC GAGGGCTTCT TGGGAGAGAA AAGCATGCTG 
TCCGGAAGCG TTCTTTCTCA AAGTTCTCTG GAGGTAGAGA ACCTGGAAGG CAGCAGAGCC 
AAGGGCAGAG CTGCAACCTC CTTTTCTCTC TCGGACGTCT CCGCGCTCTG TTCTGACATC 
CCTGATTTAC ATTCAACTGC CATTCTACAG GACACTGAAA TTTCCAATCT CATTGATGAC 
GTGACTTTAA CAAATGAGAA TCAGTCGGGA AGTTCCATCT CGGCACTGAT TGGCCAGTTT 
GAAGAAAGCA ACCACCCGGC TAACGTTACT GTGGTTTCTC ATCTCAGCAC CAGTGGGGCA 
TCAGGAAGTG CTCCTTTCCA AACACCCTTC AAGCATGGTC TTTCCCAGGG GAACCAGAAG 
GCCTCCTTCT TGTGCTCATC TCCTGAGCTG AATAAGCTCT CTAGTGTTGA GACCACCAAA 
CTGGCAAATA ACGCAGTTCC TTGTGGAGTG ATTGGTTCTC CCATCTCTAC ACCAAAGCCA 
GGTGATGACC CTTCAGATAA GGCCAAGACA AGAGTCATTG AAGGCAACCT GCCTGGGTTC 
CCTGATGCTT CTCCTGGTCA ATTTCCCAAA AGCCCCACCC ATGGAGAGGA CCATAGTCAA 
GTGATGAACA GCCCTGCTCT CTCCACGGAG TTGGCCATAG AAGATATAAT TGCAGACCCT 
GCTCTCTCTA TAAATTCTGC AGAAAGCAGT CTTGTGGAAA TTGATGGAGA ATCTGAAAAC 
CTATCTCTAA CAACCTGTGA CTACAGGGAA GAAGCTCCAA GTCAACTTGT TTCTCCACTA 
AAACTGCAGC AGAGCCAGGA GATGGTGGAA CACATTCAGA GGGGCTTGAG GAATGGCTAC 
TGTAAAGAGA CTCTCCTCCC TTCTGAAATA TTCAACAATA TTCCAGGTGT CAAAAATCAC 
AGTATTTCTC ATCTAACCTA TCAGGGTGCT GGCTTTGTGT ATAACCATTT CTCAAGTTCA 
GATGCAAAAA CGAACCAAAT CTGTGAGCCC CAGCAGCCTA GGGCTCCAGA TATGCATGCC 
CCTACACCTA CACCATCAAC ACATGCTCCT TTGGCTGCTT TGAAACTGCC AAGCCCATGC 
AAATCCAAAA GTCTGGGGGA CTTAACATCA GAGGACATTG CCTGCAATTT TGAAAGCAAA 
TATCAATGTA TTAGTAGGAG CTTTGTGACA AACGGCATTA GGGACAAGAG CGTGACTATG 
AAGACAAAGT CATTAGAGCC TTTAGACGCC CTGACTGAAC AGCTCCGGAA GCTGGTGTCC 
TTTGACCAAG AAGACAGCTG TCAAGTGCTC TACTCAAAGC AGGATGTCAA TCAGTGTCCC 
AGGGCATTAG TCAGAAAGTT GTCGTCTAGA AGTCAGAGCA GAGTGCGCAA CATTGCTAGC 
CGTGCCAAGG AGAAGCAGGA AGCTGGCAAG CAAAAAGCTA TGGCCCAGAG CACCAGAGGA 
GGAGTGGTCC TTAGAAGTAA ACCGCCCGCT CCTGCTCTGG CCGTGAACCG TCACTCCACG 
GGCTCATACA TTGCAAGCTA CCTGAGGAAC ATGAAAGCTG GTGGCCTAGA GGGTCGAGGC 
ATCCCAGAGG GAGCGTGCAC GGCCCTTCGC TATGGCTACA TGGACCAGTT TTGTTCAGAT 
AATTCAGTTC TGCAGACTGA GCCAAGCAGC GAAGATAAAC CCGAAATTTA TTTTCTTTTG 
AGGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899014.2
CDS	295..5343
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	NM_183191.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183191.4

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTAATGCAGT CCGGGACACA GATGATCAAA CTGAAGCGTG GCACCAAAGG GCTCGTTCGC 
CTCTTTTACC TGGATGAGCA CCGGACCCGC CTCCGATGGC GACCCTCTCG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATCTAC AAAGTCACCG AGGGCAGGCA GTCTGAAATA 
TTCCACAGAC AGGCTGAGGG GAACTTTGAC CCTAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA CCTCATTACC TCCAACCCAG AGGAGGCACG CACCTGGATC 
ACGGGCCTCA AGTATCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAGAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA CGGTGATGGC 
TTATTGAATA TTGAAGAGAT TCACCAGCTG ATGCATAAAC TAAATGTCAA TCTGCCCCGC 
AGAAAAGTCA GGCAAATGTT TCAGGAAGCA GATACAGATG AGAATCAGGG AACCTTGACA 
TTTGAAGAGT TCTGCGTTTT TTACAAAATG ATGTCATTGA GACGAGACCT CTATTTGCTG 
CTCTTGAGCT ACAGTGACAA GAAAGACCAC CTGACTGTGG AGGAGCTGGC TCAGTTTTTG 
AAAGTGGAAC AAAAGATGAG TAATGTGACA CTGGACTATT GTCTTGACAT CATAATGAAG 
TTTGAAGTTT CTGAAGAAAA CAAAGTGAAA AACGTCCTTG GTATAGAAGG CTTCACGAAC 
TTCATGCGTA GCCCTGCCTG TGACGTATTT AACCCGTTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTGTGCAA CTACTACATT GCTTCATCTC ACAACACATA CCTGACTGGG 
GATCAGCTCC TTTCTCAATC TAAAGTGGAT ATGTATGCAC GGGTGCTACA AGAGGGTTGT 
AGATGCGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTGGT CCACCATGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCA 
TTCGTGAAGA ATGAGTTCCC GGTTATCCTG TCCATTGAGA ACCACTGCAG CATTCAGCAG 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGT ATATTGCAGG ACAAACTGGA CCTGTCTTCT 
GTAGACACCG GAGAGTGCAG GCAGCTTCCA AGCCCTCAGA GTCTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC GTATCACCTT GGGGATGATG CAGAGGAAGG AGAAGTGTCC 
GATGAGGACA GTGCAGATGA GATTGAAGAT GAGTGCAAGT TCAAGCTGCA TTACAGTAAC 
GGGACCACTG AGCACCAGGT AGAATCTTTC ATACGGAAAA AGCTGGAGTC ACTGTTGAAG 
GAGTCTCAGA TTCGAGACAA AGAAGATCCA GACAGTTTCA CAGTGAGGGC GTTACTCAAG 
GCTACACATG AAGGCTTAAA TGCACACCTG AAGCAGAACC TGGATGTAAA GGAAAGTGGA 
AAGAAGTCCC ATGGGCGATC CCTGATGGCC AACTTTGGGA AACACAAGCA GAAAGCTACG 
AAATCACGTT CTAAATCCTA TAGTACTGAT GATGAGGATG ATAGCCTTCA GAATCCTGGC 
AAGGAGGGAG GCCAGCTGTA CAGGCTGGGC CGCCGAAGGA GAACCATGAA ACTCTGCAGA 
GAGCTCTCGG ACTTGGTGGT GTACACAAAC TCCGTGGCAG CCCAGGATAT TGTTGATGAT 
GGAACCACAG GGAATGTGTT GTCCTTCAGC GAAACAAGAG CCCATCAAGT GGTTCAGCAG 
AAGTCAGAGC AGTTCATGAT CTACAACCAA AAACAGCTCA CAAGGATTTA CCCCTCTGCC 
TACCGCATCG ATTCCAGTAA CTTCAACCCA CTGCCCTACT GGAATGCAGG CTGCCAGCTC 
GTGGCTCTGA ACTACCAGTC TGAAGGGCGA ATGATGCAGA TCAATCGAGC AAAATTCAAG 
GCGAATGGCA ACTGTGGCTA TATTCTCAAG CCCCAGCAAA TGTGCAAAGG TACTTTCAAC 
CCTTTCTCTG GTGACCCACT TCCTGCCAAC CCCAAAAAAC AGCTCATACT GAAAGTGATC 
AGTGGACAGC AACTCCCCAA ACCTCCTGAC TCTATGTTTG GAGACCGAGG CGAGATCATT 
GACCCTTTCG TTGAAGTTGA AATTATTGGG CTGCCAGTAG ACTGTTGCAA GGACCAAACT 
CGTGTGGTGG ATGACAACGG ATTTAACCCT GTGTGGGAAG AAACCCTAAC TTTTACAGTT 
CACATGCCAG AAATAGCTTT GGTTCGGTTC CTTGTGTGGG ATCATGATCC TATTGGAAGA 
GACTTTGTCG GACAAAGGAC TGTGACCTTC AGTAGCCTCG TGCCCGGCTA CCGGCATGTC 
TACCTGGAAG GACTCACAGA AGCATCTATT TTCGTTCACA TAACCATCAA TGAGATCTTT 
GGAAAGTGGA GTCCTTTAAT CCTCAACCCC AGTTATACCA TATTGCACTT TCTAGGAGCT 
ACAAAGAACA GACAGCTCCA AGGCCTGAAG GGGCTGTTCA ATAAGAATCC CAGGCACGCT 
TCTTCAGAAA ACAACTCGCA TTATGTTCGG AAGCGATCGA TTGGAGATAG GATTCTGCGA 
CGTACAGCTA GTGCTCCAGC CAAAGGCAGG AAAAAGAGCA AAGTGGGCTT CCAAGAAATG 
GTTGAGATAA AAGATTCTGT CTCTGAGGCT TCAAGAGATC AAGATGGTGT CCTGAGGAGG 
ACCACCCGGA GTCTGCAAGT ACGCCCTGTC TCTATGCCTG TTGACAAGAG CCTTCTGGGG 
GCTTTGTCGC TGCCCATATC TGAAGCAGCA AAAGACACGG ATGGAAAAGA AAACTGTCTG 
GCAGGAGACA AGGATGACAG AAGAAAGGGA GCCGCAACTA GAAAAGACCC ACATTTTTCA 
AATTTCAACA AAAAGTTATC CTCCTCCTCC AGTGCGCTCC TCCACAAAGA TGCCAACCAA 
GGGCCAACTG CCAGTGTATC AAACCCAGAA CAGTGTGGAG GACGAGGTGC AAAGAGTGAG 
AGGATCAAAC CAAATATGAC AAATGATTGC CAGGAAAACC ACAATCCCCC GAAATTCCTT 
TCTCCAAGGA AGCATTTGGC TCTGGATCCT GCAACGAAGG GACTGCAAGA GAGACTGCAT 
GGAATGAAAA CCAATGAGAA GGAGCATGCC GAGGGCTTCT TGGGAGAGAA AAGCATGCTG 
TCCGGAAGCG TTCTTTCTCA AAGTTCTCTG GAGGTAGAGA ACCTGGAAGG CAGCAGAGCC 
AAGGGCAGAG CTGCAACCTC CTTTTCTCTC TCGGACGTCT CCGCGCTCTG TTCTGACATC 
CCTGATTTAC ATTCAACTGC CATTCTACAG GACACTGAAA TTTCCAATCT CATTGATGAC 
GTGACTTTAA CAAATGAGAA TCAGTCGGGA AGTTCCATCT CGGCACTGAT TGGCCAGTTT 
GAAGAAAGCA ACCACCCGGC TAACGTTACT GTGGTTTCTC ATCTCAGCAC CAGTGGGGCA 
TCAGGAAGTG CTCCTTTCCA AACACCCTTC AAGCATGGTC TTTCCCAGGG GAACCAGAAG 
GCCTCCTTCT TGTGCTCATC TCCTGAGCTG AATAAGCTCT CTAGTGTTGA GACCACCAAA 
CTGGCAAATA ACGCAGTTCC TTGTGGAGTG ATTGGTTCTC CCATCTCTAC ACCAAAGCCA 
GGTGATGACC CTTCAGATAA GGCCAAGACA AGAGTCATTG AAGGCAACCT GCCTGGGTTC 
CCTGATGCTT CTCCTGGTCA ATTTCCCAAA AGCCCCACCC ATGGAGAGGA CCATAGTCAA 
GTGATGAACA GCCCTGCTCT CTCCACGGAG TTGGCCATAG AAGATATAAT TGCAGACCCT 
GCTCTCTCTA TAAATTCTGC AGAAAGCAGT CTTGTGGAAA TTGATGGAGA ATCTGAAAAC 
CTATCTCTAA CAACCTGTGA CTACAGGGAA GAAGCTCCAA GTCAACTTGT TTCTCCACTA 
AAACTGCAGC AGAGCCAGGA GATGGTGGAA CACATTCAGA GGGGCTTGAG GAATGGCTAC 
TGTAAAGAGA CTCTCCTCCC TTCTGAAATA TTCAACAATA TTCCAGGTGT CAAAAATCAC 
AGTATTTCTC ATCTAACCTA TCAGGGTGCT GGCTTTGTGT ATAACCATTT CTCAAGTTCA 
GATGCAAAAA CGAACCAAAT CTGTGAGCCC CAGCAGCCTA GGGCTCCAGA TATGCATGCC 
CCTACACCTA CACCATCAAC ACATGCTCCT TTGGCTGCTT TGAAACTGCC AAGCCCATGC 
AAATCCAAAA GTCTGGGGGA CTTAACATCA GAGGACATTG CCTGCAATTT TGAAAGCAAA 
TATCAATGTA TTAGTAGGAG CTTTGTGACA AACGGCATTA GGGACAAGAG CGTGACTATG 
AAGACAAAGT CATTAGAGCC TTTAGACGCC CTGACTGAAC AGCTCCGGAA GCTGGTGTCC 
TTTGACCAAG AAGACAGCTG TCAAGTGCTC TACTCAAAGC AGGATGTCAA TCAGTGTCCC 
AGGGCATTAG TCAGAAAGTT GTCGTCTAGA AGTCAGAGCA GAGTGCGCAA CATTGCTAGC 
CGTGCCAAGG AGAAGCAGGA AGCTGGCAAG CAAAAAGCTA TGGCCCAGAG CACCAGAGGA 
GGAGTGGTCC TTAGAAGTAA ACCGCCCGCT CCTGCTCTGG CCGTGAACCG TCACTCCACG 
GGCTCATACA TTGCAAGCTA CCTGAGGAAC ATGAAAGCTG GTGGCCTAGA GGGTCGAGGC 
ATCCCAGAGG GAGCGTGCAC GGCCCTTCGC TATGGCTACA TGGACCAGTT TTGTTCAGAT 
AATTCAGTTC TGCAGACTGA GCCAAGCAGC GAAGATAAAC CCGAAATTTA TTTTCTTTTG 
AGGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( NM_001370984.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001357913.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 4, mRNA.

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CloneID	OMu31951
Clone ID Related Accession (Same CDS sequence)	XM_006501537.3 , NM_001370984.1
Accession Version	NM_001370984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-19
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116732.10 and AC121285.7. On Jun 20, 2019 this sequence version replaced XM_006501537.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5314783.21104.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357913.1
CDS	363..5387
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	NM_001370984.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370984.1

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501530.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501593.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X1, mRNA.

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CloneID	OMu31948
Clone ID Related Accession (Same CDS sequence)	XM_011240131.3 , XM_006501533.4 , XM_006501530.3 , XM_006501531.3 , XM_017319593.1 , XM_006501530.4 , XM_006501531.4 , XM_006501533.3 , XM_017319593.2 , XM_017319594.1 , XM_011240131.2
Accession Version	XM_006501530.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501530.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501593.1
CDS	586..5670
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006501530.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501530.3

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501531.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501594.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X4, mRNA.

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CloneID	OMu31948
Clone ID Related Accession (Same CDS sequence)	XM_011240131.3 , XM_006501533.4 , XM_006501530.3 , XM_006501531.3 , XM_017319593.1 , XM_006501530.4 , XM_006501531.4 , XM_006501533.3 , XM_017319593.2 , XM_017319594.1 , XM_011240131.2
Accession Version	XM_006501531.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501531.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501594.1
CDS	1740..6824
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006501531.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501531.3

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319593.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175082.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X3, mRNA.

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CloneID	OMu31948
Clone ID Related Accession (Same CDS sequence)	XM_011240131.3 , XM_006501533.4 , XM_006501530.3 , XM_006501531.3 , XM_017319593.1 , XM_006501530.4 , XM_006501531.4 , XM_006501533.3 , XM_017319593.2 , XM_017319594.1 , XM_011240131.2
Accession Version	XM_017319593.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175082.1
CDS	714..5798
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_017319593.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319593.1

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501533.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501596.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X6, mRNA.

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CloneID	OMu31948
Clone ID Related Accession (Same CDS sequence)	XM_011240131.3 , XM_006501533.4 , XM_006501530.3 , XM_006501531.3 , XM_017319593.1 , XM_006501530.4 , XM_006501531.4 , XM_006501533.3 , XM_017319593.2 , XM_017319594.1 , XM_011240131.2
Accession Version	XM_006501533.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501533.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501596.1
CDS	500..5584
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006501533.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501533.3

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_017319594.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175083.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu31948
Clone ID Related Accession (Same CDS sequence)	XM_011240131.3 , XM_006501533.4 , XM_006501530.3 , XM_006501531.3 , XM_017319593.1 , XM_006501530.4 , XM_006501531.4 , XM_006501533.3 , XM_017319593.2 , XM_017319594.1 , XM_011240131.2
Accession Version	XM_017319594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175083.1
CDS	146..5230
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_017319594.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319594.1

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_011240131.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238433.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X2, mRNA.

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CloneID	OMu31948
Clone ID Related Accession (Same CDS sequence)	XM_011240131.3 , XM_006501533.4 , XM_006501530.3 , XM_006501531.3 , XM_017319593.1 , XM_006501530.4 , XM_006501531.4 , XM_006501533.3 , XM_017319593.2 , XM_017319594.1 , XM_011240131.2
Accession Version	XM_011240131.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240131.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238433.1
CDS	676..5760
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_011240131.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240131.2

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501535.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501598.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X7, mRNA.

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CloneID	OMu31949
Clone ID Related Accession (Same CDS sequence)	XM_006501535.3 , XM_006501535.4
Accession Version	XM_006501535.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501535.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGT GGAGTCCTTT AATCCTCAAC 
CCCAGTTATA CCATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGCAGGAG ACAAGGATGA CAGAAGAAAG 
GGAGCCGCAA CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC 
TCCAGTGCGC TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA 
GAACAGTGTG GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GACAAATGAT 
TGCCAGGAAA ACCACAATCC CCCGAAATTC CTTTCTCCAA GGAAGCATTT GGCTCTGGAT 
CCTGCAACGA AGGGACTGCA AGAGAGACTG CATGGAATGA AAACCAATGA GAAGGAGCAT 
GCCGAGGGCT TCTTGGGAGA GAAAAGCATG CTGTCCGGAA GCGTTCTTTC TCAAAGTTCT 
CTGGAGGTAG AGAACCTGGA AGGCAGCAGA GCCAAGGGCA GAGCTGCAAC CTCCTTTTCT 
CTCTCGGACG TCTCCGCGCT CTGTTCTGAC ATCCCTGATT TACATTCAAC TGCCATTCTA 
CAGGACACTG AAATTTCCAA TCTCATTGAT GACGTGACTT TAACAAATGA GAATCAGTCG 
GGAAGTTCCA TCTCGGCACT GATTGGCCAG TTTGAAGAAA GCAACCACCC GGCTAACGTT 
ACTGTGGTTT CTCATCTCAG CACCAGTGGG GCATCAGGAA GTGCTCCTTT CCAAACACCC 
TTCAAGCATG GTCTTTCCCA GGGGAACCAG AAGGCCTCCT TCTTGTGCTC ATCTCCTGAG 
CTGAATAAGC TCTCTAGTGT TGAGACCACC AAACTGGCAA ATAACGCAGT TCCTTGTGGA 
GTGATTGGTT CTCCCATCTC TACACCAAAG CCAGGTGATG ACCCTTCAGA TAAGGCCAAG 
ACAAGAGTCA TTGAAGGCAA CCTGCCTGGG TTCCCTGATG CTTCTCCTGG TCAATTTCCC 
AAAAGCCCCA CCCATGGAGA GGACCATAGT CAAGTGATGA ACAGCCCTGC TCTCTCCACG 
GAGTTGGCCA TAGAAGATAT AATTGCAGAC CCTGCTCTCT CTATAAATTC TGCAGAAAGC 
AGTCTTGTGG AAATTGATGG AGAATCTGAA AACCTATCTC TAACAACCTG TGACTACAGG 
GAAGAAGCTC CAAGTCAACT TGTTTCTCCA CTAAAACTGC AGCAGAGCCA GGAGATGGTG 
GAACACATTC AGAGGGGCTT GAGGAATGGC TACTGTAAAG AGACTCTCCT CCCTTCTGAA 
ATATTCAACA ATATTCCAGG TGTCAAAAAT CACAGTATTT CTCATCTAAC CTATCAGGGT 
GCTGGCTTTG TGTATAACCA TTTCTCAAGT TCAGATGCAA AAACGAACCA AATCTGTGAG 
CCCCAGCAGC CTAGGGCTCC AGATATGCAT GCCCCTACAC CTACACCATC AACACATGCT 
CCTTTGGCTG CTTTGAAACT GCCAAGCCCA TGCAAATCCA AAAGTCTGGG GGACTTAACA 
TCAGAGGACA TTGCCTGCAA TTTTGAAAGC AAATATCAAT GTATTAGTAG GAGCTTTGTG 
ACAAACGGCA TTAGGGACAA GAGCGTGACT ATGAAGACAA AGTCATTAGA GCCTTTAGAC 
GCCCTGACTG AACAGCTCCG GAAGCTGGTG TCCTTTGACC AAGAAGACAG CTGTCAAGTG 
CTCTACTCAA AGCAGGATGT CAATCAGTGT CCCAGGGCAT TAGTCAGAAA GTTGTCGTCT 
AGAAGTCAGA GCAGAGTGCG CAACATTGCT AGCCGTGCCA AGGAGAAGCA GGAAGCTGGC 
AAGCAAAAAG CTATGGCCCA GAGCACCAGA GGAGGAGTGG TCCTTAGAAG TAAACCGCCC 
GCTCCTGCTC TGGCCGTGAA CCGTCACTCC ACGGGCTCAT ACATTGCAAG CTACCTGAGG 
AACATGAAAG CTGGTGGCCT AGAGGGTCGA GGCATCCCAG AGGGAGCGTG CACGGCCCTT 
CGCTATGGCT ACATGGACCA GTTTTGTTCA GATAATTCAG TTCTGCAGAC TGAGCCAAGC 
AGCGAAGATA AACCCGAAAT TTATTTTCTT TTGAGGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501598.1
CDS	586..5667
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006501535.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501535.3

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGT GGAGTCCTTT AATCCTCAAC 
CCCAGTTATA CCATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGCAGGAG ACAAGGATGA CAGAAGAAAG 
GGAGCCGCAA CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC 
TCCAGTGCGC TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA 
GAACAGTGTG GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GACAAATGAT 
TGCCAGGAAA ACCACAATCC CCCGAAATTC CTTTCTCCAA GGAAGCATTT GGCTCTGGAT 
CCTGCAACGA AGGGACTGCA AGAGAGACTG CATGGAATGA AAACCAATGA GAAGGAGCAT 
GCCGAGGGCT TCTTGGGAGA GAAAAGCATG CTGTCCGGAA GCGTTCTTTC TCAAAGTTCT 
CTGGAGGTAG AGAACCTGGA AGGCAGCAGA GCCAAGGGCA GAGCTGCAAC CTCCTTTTCT 
CTCTCGGACG TCTCCGCGCT CTGTTCTGAC ATCCCTGATT TACATTCAAC TGCCATTCTA 
CAGGACACTG AAATTTCCAA TCTCATTGAT GACGTGACTT TAACAAATGA GAATCAGTCG 
GGAAGTTCCA TCTCGGCACT GATTGGCCAG TTTGAAGAAA GCAACCACCC GGCTAACGTT 
ACTGTGGTTT CTCATCTCAG CACCAGTGGG GCATCAGGAA GTGCTCCTTT CCAAACACCC 
TTCAAGCATG GTCTTTCCCA GGGGAACCAG AAGGCCTCCT TCTTGTGCTC ATCTCCTGAG 
CTGAATAAGC TCTCTAGTGT TGAGACCACC AAACTGGCAA ATAACGCAGT TCCTTGTGGA 
GTGATTGGTT CTCCCATCTC TACACCAAAG CCAGGTGATG ACCCTTCAGA TAAGGCCAAG 
ACAAGAGTCA TTGAAGGCAA CCTGCCTGGG TTCCCTGATG CTTCTCCTGG TCAATTTCCC 
AAAAGCCCCA CCCATGGAGA GGACCATAGT CAAGTGATGA ACAGCCCTGC TCTCTCCACG 
GAGTTGGCCA TAGAAGATAT AATTGCAGAC CCTGCTCTCT CTATAAATTC TGCAGAAAGC 
AGTCTTGTGG AAATTGATGG AGAATCTGAA AACCTATCTC TAACAACCTG TGACTACAGG 
GAAGAAGCTC CAAGTCAACT TGTTTCTCCA CTAAAACTGC AGCAGAGCCA GGAGATGGTG 
GAACACATTC AGAGGGGCTT GAGGAATGGC TACTGTAAAG AGACTCTCCT CCCTTCTGAA 
ATATTCAACA ATATTCCAGG TGTCAAAAAT CACAGTATTT CTCATCTAAC CTATCAGGGT 
GCTGGCTTTG TGTATAACCA TTTCTCAAGT TCAGATGCAA AAACGAACCA AATCTGTGAG 
CCCCAGCAGC CTAGGGCTCC AGATATGCAT GCCCCTACAC CTACACCATC AACACATGCT 
CCTTTGGCTG CTTTGAAACT GCCAAGCCCA TGCAAATCCA AAAGTCTGGG GGACTTAACA 
TCAGAGGACA TTGCCTGCAA TTTTGAAAGC AAATATCAAT GTATTAGTAG GAGCTTTGTG 
ACAAACGGCA TTAGGGACAA GAGCGTGACT ATGAAGACAA AGTCATTAGA GCCTTTAGAC 
GCCCTGACTG AACAGCTCCG GAAGCTGGTG TCCTTTGACC AAGAAGACAG CTGTCAAGTG 
CTCTACTCAA AGCAGGATGT CAATCAGTGT CCCAGGGCAT TAGTCAGAAA GTTGTCGTCT 
AGAAGTCAGA GCAGAGTGCG CAACATTGCT AGCCGTGCCA AGGAGAAGCA GGAAGCTGGC 
AAGCAAAAAG CTATGGCCCA GAGCACCAGA GGAGGAGTGG TCCTTAGAAG TAAACCGCCC 
GCTCCTGCTC TGGCCGTGAA CCGTCACTCC ACGGGCTCAT ACATTGCAAG CTACCTGAGG 
AACATGAAAG CTGGTGGCCT AGAGGGTCGA GGCATCCCAG AGGGAGCGTG CACGGCCCTT 
CGCTATGGCT ACATGGACCA GTTTTGTTCA GATAATTCAG TTCTGCAGAC TGAGCCAAGC 
AGCGAAGATA AACCCGAAAT TTATTTTCTT TTGAGGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501536.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501599.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X8, mRNA.

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CloneID	OMu31950
Clone ID Related Accession (Same CDS sequence)	XM_006501536.3 , XM_006501536.4
Accession Version	XM_006501536.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501536.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGGAG ACAAGGATGA CAGAAGAAAG 
GGAGCCGCAA CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC 
TCCAGTGCGC TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA 
GAACAGTGTG GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GACAAATGAT 
TGCCAGGAAA ACCACAATCC CCCGAAATTC CTTTCTCCAA GGAAGCATTT GGCTCTGGAT 
CCTGCAACGA AGGGACTGCA AGAGAGACTG CATGGAATGA AAACCAATGA GAAGGAGCAT 
GCCGAGGGCT TCTTGGGAGA GAAAAGCATG CTGTCCGGAA GCGTTCTTTC TCAAAGTTCT 
CTGGAGGTAG AGAACCTGGA AGGCAGCAGA GCCAAGGGCA GAGCTGCAAC CTCCTTTTCT 
CTCTCGGACG TCTCCGCGCT CTGTTCTGAC ATCCCTGATT TACATTCAAC TGCCATTCTA 
CAGGACACTG AAATTTCCAA TCTCATTGAT GACGTGACTT TAACAAATGA GAATCAGTCG 
GGAAGTTCCA TCTCGGCACT GATTGGCCAG TTTGAAGAAA GCAACCACCC GGCTAACGTT 
ACTGTGGTTT CTCATCTCAG CACCAGTGGG GCATCAGGAA GTGCTCCTTT CCAAACACCC 
TTCAAGCATG GTCTTTCCCA GGGGAACCAG AAGGCCTCCT TCTTGTGCTC ATCTCCTGAG 
CTGAATAAGC TCTCTAGTGT TGAGACCACC AAACTGGCAA ATAACGCAGT TCCTTGTGGA 
GTGATTGGTT CTCCCATCTC TACACCAAAG CCAGGTGATG ACCCTTCAGA TAAGGCCAAG 
ACAAGAGTCA TTGAAGGCAA CCTGCCTGGG TTCCCTGATG CTTCTCCTGG TCAATTTCCC 
AAAAGCCCCA CCCATGGAGA GGACCATAGT CAAGTGATGA ACAGCCCTGC TCTCTCCACG 
GAGTTGGCCA TAGAAGATAT AATTGCAGAC CCTGCTCTCT CTATAAATTC TGCAGAAAGC 
AGTCTTGTGG AAATTGATGG AGAATCTGAA AACCTATCTC TAACAACCTG TGACTACAGG 
GAAGAAGCTC CAAGTCAACT TGTTTCTCCA CTAAAACTGC AGCAGAGCCA GGAGATGGTG 
GAACACATTC AGAGGGGCTT GAGGAATGGC TACTGTAAAG AGACTCTCCT CCCTTCTGAA 
ATATTCAACA ATATTCCAGG TGTCAAAAAT CACAGTATTT CTCATCTAAC CTATCAGGGT 
GCTGGCTTTG TGTATAACCA TTTCTCAAGT TCAGATGCAA AAACGAACCA AATCTGTGAG 
CCCCAGCAGC CTAGGGCTCC AGATATGCAT GCCCCTACAC CTACACCATC AACACATGCT 
CCTTTGGCTG CTTTGAAACT GCCAAGCCCA TGCAAATCCA AAAGTCTGGG GGACTTAACA 
TCAGAGGACA TTGCCTGCAA TTTTGAAAGC AAATATCAAT GTATTAGTAG GAGCTTTGTG 
ACAAACGGCA TTAGGGACAA GAGCGTGACT ATGAAGACAA AGTCATTAGA GCCTTTAGAC 
GCCCTGACTG AACAGCTCCG GAAGCTGGTG TCCTTTGACC AAGAAGACAG CTGTCAAGTG 
CTCTACTCAA AGCAGGATGT CAATCAGTGT CCCAGGGCAT TAGTCAGAAA GTTGTCGTCT 
AGAAGTCAGA GCAGAGTGCG CAACATTGCT AGCCGTGCCA AGGAGAAGCA GGAAGCTGGC 
AAGCAAAAAG CTATGGCCCA GAGCACCAGA GGAGGAGTGG TCCTTAGAAG TAAACCGCCC 
GCTCCTGCTC TGGCCGTGAA CCGTCACTCC ACGGGCTCAT ACATTGCAAG CTACCTGAGG 
AACATGAAAG CTGGTGGCCT AGAGGGTCGA GGCATCCCAG AGGGAGCGTG CACGGCCCTT 
CGCTATGGCT ACATGGACCA GTTTTGTTCA GATAATTCAG TTCTGCAGAC TGAGCCAAGC 
AGCGAAGATA AACCCGAAAT TTATTTTCTT TTGAGGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501599.1
CDS	589..5670
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006501536.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501536.3

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGTGGAGTCC TTTAATCCTC 
AACCCCAGTT ATACCATATT GCACTTTCTA GGAGCTACAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGGAG ACAAGGATGA CAGAAGAAAG 
GGAGCCGCAA CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC 
TCCAGTGCGC TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA 
GAACAGTGTG GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GACAAATGAT 
TGCCAGGAAA ACCACAATCC CCCGAAATTC CTTTCTCCAA GGAAGCATTT GGCTCTGGAT 
CCTGCAACGA AGGGACTGCA AGAGAGACTG CATGGAATGA AAACCAATGA GAAGGAGCAT 
GCCGAGGGCT TCTTGGGAGA GAAAAGCATG CTGTCCGGAA GCGTTCTTTC TCAAAGTTCT 
CTGGAGGTAG AGAACCTGGA AGGCAGCAGA GCCAAGGGCA GAGCTGCAAC CTCCTTTTCT 
CTCTCGGACG TCTCCGCGCT CTGTTCTGAC ATCCCTGATT TACATTCAAC TGCCATTCTA 
CAGGACACTG AAATTTCCAA TCTCATTGAT GACGTGACTT TAACAAATGA GAATCAGTCG 
GGAAGTTCCA TCTCGGCACT GATTGGCCAG TTTGAAGAAA GCAACCACCC GGCTAACGTT 
ACTGTGGTTT CTCATCTCAG CACCAGTGGG GCATCAGGAA GTGCTCCTTT CCAAACACCC 
TTCAAGCATG GTCTTTCCCA GGGGAACCAG AAGGCCTCCT TCTTGTGCTC ATCTCCTGAG 
CTGAATAAGC TCTCTAGTGT TGAGACCACC AAACTGGCAA ATAACGCAGT TCCTTGTGGA 
GTGATTGGTT CTCCCATCTC TACACCAAAG CCAGGTGATG ACCCTTCAGA TAAGGCCAAG 
ACAAGAGTCA TTGAAGGCAA CCTGCCTGGG TTCCCTGATG CTTCTCCTGG TCAATTTCCC 
AAAAGCCCCA CCCATGGAGA GGACCATAGT CAAGTGATGA ACAGCCCTGC TCTCTCCACG 
GAGTTGGCCA TAGAAGATAT AATTGCAGAC CCTGCTCTCT CTATAAATTC TGCAGAAAGC 
AGTCTTGTGG AAATTGATGG AGAATCTGAA AACCTATCTC TAACAACCTG TGACTACAGG 
GAAGAAGCTC CAAGTCAACT TGTTTCTCCA CTAAAACTGC AGCAGAGCCA GGAGATGGTG 
GAACACATTC AGAGGGGCTT GAGGAATGGC TACTGTAAAG AGACTCTCCT CCCTTCTGAA 
ATATTCAACA ATATTCCAGG TGTCAAAAAT CACAGTATTT CTCATCTAAC CTATCAGGGT 
GCTGGCTTTG TGTATAACCA TTTCTCAAGT TCAGATGCAA AAACGAACCA AATCTGTGAG 
CCCCAGCAGC CTAGGGCTCC AGATATGCAT GCCCCTACAC CTACACCATC AACACATGCT 
CCTTTGGCTG CTTTGAAACT GCCAAGCCCA TGCAAATCCA AAAGTCTGGG GGACTTAACA 
TCAGAGGACA TTGCCTGCAA TTTTGAAAGC AAATATCAAT GTATTAGTAG GAGCTTTGTG 
ACAAACGGCA TTAGGGACAA GAGCGTGACT ATGAAGACAA AGTCATTAGA GCCTTTAGAC 
GCCCTGACTG AACAGCTCCG GAAGCTGGTG TCCTTTGACC AAGAAGACAG CTGTCAAGTG 
CTCTACTCAA AGCAGGATGT CAATCAGTGT CCCAGGGCAT TAGTCAGAAA GTTGTCGTCT 
AGAAGTCAGA GCAGAGTGCG CAACATTGCT AGCCGTGCCA AGGAGAAGCA GGAAGCTGGC 
AAGCAAAAAG CTATGGCCCA GAGCACCAGA GGAGGAGTGG TCCTTAGAAG TAAACCGCCC 
GCTCCTGCTC TGGCCGTGAA CCGTCACTCC ACGGGCTCAT ACATTGCAAG CTACCTGAGG 
AACATGAAAG CTGGTGGCCT AGAGGGTCGA GGCATCCCAG AGGGAGCGTG CACGGCCCTT 
CGCTATGGCT ACATGGACCA GTTTTGTTCA GATAATTCAG TTCTGCAGAC TGAGCCAAGC 
AGCGAAGATA AACCCGAAAT TTATTTTCTT TTGAGGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501537.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501600.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X9, mRNA.

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CloneID	OMu31951
Clone ID Related Accession (Same CDS sequence)	XM_006501537.3 , NM_001370984.1
Accession Version	XM_006501537.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501537.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501600.1
CDS	582..5606
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006501537.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501537.3

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGCAGAAA GCTACGAAAT CACGTTCTAA ATCCTATAGT 
ACTGATGATG AGGATGATAG CCTTCAGAAT CCTGGCAAGG AGGGAGGCCA GCTGTACAGG 
CTGGGCCGCC GAAGGAGAAC CATGAAACTC TGCAGAGAGC TCTCGGACTT GGTGGTGTAC 
ACAAACTCCG TGGCAGCCCA GGATATTGTT GATGATGGAA CCACAGGGAA TGTGTTGTCC 
TTCAGCGAAA CAAGAGCCCA TCAAGTGGTT CAGCAGAAGT CAGAGCAGTT CATGATCTAC 
AACCAAAAAC AGCTCACAAG GATTTACCCC TCTGCCTACC GCATCGATTC CAGTAACTTC 
AACCCACTGC CCTACTGGAA TGCAGGCTGC CAGCTCGTGG CTCTGAACTA CCAGTCTGAA 
GGGCGAATGA TGCAGATCAA TCGAGCAAAA TTCAAGGCGA ATGGCAACTG TGGCTATATT 
CTCAAGCCCC AGCAAATGTG CAAAGGTACT TTCAACCCTT TCTCTGGTGA CCCACTTCCT 
GCCAACCCCA AAAAACAGCT CATACTGAAA GTGATCAGTG GACAGCAACT CCCCAAACCT 
CCTGACTCTA TGTTTGGAGA CCGAGGCGAG ATCATTGACC CTTTCGTTGA AGTTGAAATT 
ATTGGGCTGC CAGTAGACTG TTGCAAGGAC CAAACTCGTG TGGTGGATGA CAACGGATTT 
AACCCTGTGT GGGAAGAAAC CCTAACTTTT ACAGTTCACA TGCCAGAAAT AGCTTTGGTT 
CGGTTCCTTG TGTGGGATCA TGATCCTATT GGAAGAGACT TTGTCGGACA AAGGACTGTG 
ACCTTCAGTA GCCTCGTGCC CGGCTACCGG CATGTCTACC TGGAAGGACT CACAGAAGCA 
TCTATTTTCG TTCACATAAC CATCAATGAG ATCTTTGGAA AGAACAGACA GCTCCAAGGC 
CTGAAGGGGC TGTTCAATAA GAATCCCAGG CACGCTTCTT CAGAAAACAA CTCGCATTAT 
GTTCGGAAGC GATCGATTGG AGATAGGATT CTGCGACGTA CAGCTAGTGC TCCAGCCAAA 
GGCAGGAAAA AGAGCAAAGT GGGCTTCCAA GAAATGGTTG AGATAAAAGA TTCTGTCTCT 
GAGGCTTCAA GAGATCAAGA TGGTGTCCTG AGGAGGACCA CCCGGAGTCT GCAAGTACGC 
CCTGTCTCTA TGCCTGTTGA CAAGAGCCTT CTGGGGGCTT TGTCGCTGCC CATATCTGAA 
GCAGCAAAAG ACACGGATGG AAAAGAAAAC TGTCTGGCAG GAGACAAGGA TGACAGAAGA 
AAGGGAGCCG CAACTAGAAA AGACCCACAT TTTTCAAATT TCAACAAAAA GTTATCCTCC 
TCCTCCAGTG CGCTCCTCCA CAAAGATGCC AACCAAGGGC CAACTGCCAG TGTATCAAAC 
CCAGAACAGT GTGGAGGACG AGGTGCAAAG AGTGAGAGGA TCAAACCAAA TATGACAAAT 
GATTGCCAGG AAAACCACAA TCCCCCGAAA TTCCTTTCTC CAAGGAAGCA TTTGGCTCTG 
GATCCTGCAA CGAAGGGACT GCAAGAGAGA CTGCATGGAA TGAAAACCAA TGAGAAGGAG 
CATGCCGAGG GCTTCTTGGG AGAGAAAAGC ATGCTGTCCG GAAGCGTTCT TTCTCAAAGT 
TCTCTGGAGG TAGAGAACCT GGAAGGCAGC AGAGCCAAGG GCAGAGCTGC AACCTCCTTT 
TCTCTCTCGG ACGTCTCCGC GCTCTGTTCT GACATCCCTG ATTTACATTC AACTGCCATT 
CTACAGGACA CTGAAATTTC CAATCTCATT GATGACGTGA CTTTAACAAA TGAGAATCAG 
TCGGGAAGTT CCATCTCGGC ACTGATTGGC CAGTTTGAAG AAAGCAACCA CCCGGCTAAC 
GTTACTGTGG TTTCTCATCT CAGCACCAGT GGGGCATCAG GAAGTGCTCC TTTCCAAACA 
CCCTTCAAGC ATGGTCTTTC CCAGGGGAAC CAGAAGGCCT CCTTCTTGTG CTCATCTCCT 
GAGCTGAATA AGCTCTCTAG TGTTGAGACC ACCAAACTGG CAAATAACGC AGTTCCTTGT 
GGAGTGATTG GTTCTCCCAT CTCTACACCA AAGCCAGGTG ATGACCCTTC AGATAAGGCC 
AAGACAAGAG TCATTGAAGG CAACCTGCCT GGGTTCCCTG ATGCTTCTCC TGGTCAATTT 
CCCAAAAGCC CCACCCATGG AGAGGACCAT AGTCAAGTGA TGAACAGCCC TGCTCTCTCC 
ACGGAGTTGG CCATAGAAGA TATAATTGCA GACCCTGCTC TCTCTATAAA TTCTGCAGAA 
AGCAGTCTTG TGGAAATTGA TGGAGAATCT GAAAACCTAT CTCTAACAAC CTGTGACTAC 
AGGGAAGAAG CTCCAAGTCA ACTTGTTTCT CCACTAAAAC TGCAGCAGAG CCAGGAGATG 
GTGGAACACA TTCAGAGGGG CTTGAGGAAT GGCTACTGTA AAGAGACTCT CCTCCCTTCT 
GAAATATTCA ACAATATTCC AGGTGTCAAA AATCACAGTA TTTCTCATCT AACCTATCAG 
GGTGCTGGCT TTGTGTATAA CCATTTCTCA AGTTCAGATG CAAAAACGAA CCAAATCTGT 
GAGCCCCAGC AGCCTAGGGC TCCAGATATG CATGCCCCTA CACCTACACC ATCAACACAT 
GCTCCTTTGG CTGCTTTGAA ACTGCCAAGC CCATGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAA AGCAAATATC AATGTATTAG TAGGAGCTTT 
GTGACAAACG GCATTAGGGA CAAGAGCGTG ACTATGAAGA CAAAGTCATT AGAGCCTTTA 
GACGCCCTGA CTGAACAGCT CCGGAAGCTG GTGTCCTTTG ACCAAGAAGA CAGCTGTCAA 
GTGCTCTACT CAAAGCAGGA TGTCAATCAG TGTCCCAGGG CATTAGTCAG AAAGTTGTCG 
TCTAGAAGTC AGAGCAGAGT GCGCAACATT GCTAGCCGTG CCAAGGAGAA GCAGGAAGCT 
GGCAAGCAAA AAGCTATGGC CCAGAGCACC AGAGGAGGAG TGGTCCTTAG AAGTAAACCG 
CCCGCTCCTG CTCTGGCCGT GAACCGTCAC TCCACGGGCT CATACATTGC AAGCTACCTG 
AGGAACATGA AAGCTGGTGG CCTAGAGGGT CGAGGCATCC CAGAGGGAGC GTGCACGGCC 
CTTCGCTATG GCTACATGGA CCAGTTTTGT TCAGATAATT CAGTTCTGCA GACTGAGCCA 
AGCAGCGAAG ATAAACCCGA AATTTATTTT CTTTTGAGGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_006501538.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501601.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu31952
Clone ID Related Accession (Same CDS sequence)	XM_006501538.4 , XM_006501538.3
Accession Version	XM_006501538.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501538.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGCAGGAG ACAAGGATGA CAGAAGAAAG 
GGAGCCGCAA CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC 
TCCAGTGCGC TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA 
GAACAGTGTG GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GACAAATGAT 
TGCCAGGAAA ACCACAATCC CCCGAAATTC CTTTCTCCAA GGAAGCATTT GGCTCTGGAT 
CCTGCAACGA AGGGACTGCA AGAGAGACTG CATGGAATGA AAACCAATGA GAAGGAGCAT 
GCCGAGGGCT TCTTGGGAGA GAAAAGCATG CTGTCCGGAA GCGTTCTTTC TCAAAGTTCT 
CTGGAGGTAG AGAACCTGGA AGGCAGCAGA GCCAAGGGCA GAGCTGCAAC CTCCTTTTCT 
CTCTCGGACG TCTCCGCGCT CTGTTCTGAC ATCCCTGATT TACATTCAAC TGCCATTCTA 
CAGGACACTG AAATTTCCAA TCTCATTGAT GACGTGACTT TAACAAATGA GAATCAGTCG 
GGAAGTTCCA TCTCGGCACT GATTGGCCAG TTTGAAGAAA GCAACCACCC GGCTAACGTT 
ACTGTGGTTT CTCATCTCAG CACCAGTGGG GCATCAGGAA GTGCTCCTTT CCAAACACCC 
TTCAAGCATG GTCTTTCCCA GGGGAACCAG AAGGCCTCCT TCTTGTGCTC ATCTCCTGAG 
CTGAATAAGC TCTCTAGTGT TGAGACCACC AAACTGGCAA ATAACGCAGT TCCTTGTGGA 
GTGATTGGTT CTCCCATCTC TACACCAAAG CCAGGTGATG ACCCTTCAGA TAAGGCCAAG 
ACAAGAGTCA TTGAAGGCAA CCTGCCTGGG TTCCCTGATG CTTCTCCTGG TCAATTTCCC 
AAAAGCCCCA CCCATGGAGA GGACCATAGT CAAGTGATGA ACAGCCCTGC TCTCTCCACG 
GAGTTGGCCA TAGAAGATAT AATTGCAGAC CCTGCTCTCT CTATAAATTC TGCAGAAAGC 
AGTCTTGTGG AAATTGATGG AGAATCTGAA AACCTATCTC TAACAACCTG TGACTACAGG 
GAAGAAGCTC CAAGTCAACT TGTTTCTCCA CTAAAACTGC AGCAGAGCCA GGAGATGGTG 
GAACACATTC AGAGGGGCTT GAGGAATGGC TACTGTAAAG AGACTCTCCT CCCTTCTGAA 
ATATTCAACA ATATTCCAGG TGTCAAAAAT CACAGTATTT CTCATCTAAC CTATCAGGGT 
GCTGGCTTTG TGTATAACCA TTTCTCAAGT TCAGATGCAA AAACGAACCA AATCTGTGAG 
CCCCAGCAGC CTAGGGCTCC AGATATGCAT GCCCCTACAC CTACACCATC AACACATGCT 
CCTTTGGCTG CTTTGAAACT GCCAAGCCCA TGCAAATCCA AAAGTCTGGG GGACTTAACA 
TCAGAGGACA TTGCCTGCAA TTTTGAAAGC AAATATCAAT GTATTAGTAG GAGCTTTGTG 
ACAAACGGCA TTAGGGACAA GAGCGTGACT ATGAAGACAA AGTCATTAGA GCCTTTAGAC 
GCCCTGACTG AACAGCTCCG GAAGCTGGTG TCCTTTGACC AAGAAGACAG CTGTCAAGTG 
CTCTACTCAA AGCAGGATGT CAATCAGTGT CCCAGGGCAT TAGTCAGAAA GTTGTCGTCT 
AGAAGTCAGA GCAGAGTGCG CAACATTGCT AGCCGTGCCA AGGAGAAGCA GGAAGCTGGC 
AAGCAAAAAG CTATGGCCCA GAGCACCAGA GGAGGAGTGG TCCTTAGAAG TAAACCGCCC 
GCTCCTGCTC TGGCCGTGAA CCGTCACTCC ACGGGCTCAT ACATTGCAAG CTACCTGAGG 
AACATGAAAG CTGGTGGCCT AGAGGGTCGA GGCATCCCAG AGGGAGCGTG CACGGCCCTT 
CGCTATGGCT ACATGGACCA GTTTTGTTCA GATAATTCAG TTCTGCAGAC TGAGCCAAGC 
AGCGAAGATA AACCCGAAAT TTATTTTCTT TTGAGGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501601.1
CDS	582..5603
Translation	MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006501538.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501538.3

ORF Insert Sequence:

ATGAACTACT GGAACGGTGA GAAGAGGAAC TGTGTGCAGT ACCGCAGACA TTTTCTGGTG 
GACAACCGCG TGTTTCATGT TGAAAGATGC ATGAGTGTAA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AGCGTGGCAC CAAAGGGCTC GTTCGCCTCT TTTACCTGGA TGAGCACCGG 
ACCCGCCTCC GATGGCGACC CTCTCGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATCTACAAAG TCACCGAGGG CAGGCAGTCT GAAATATTCC ACAGACAGGC TGAGGGGAAC 
TTTGACCCTA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGACCTC 
ATTACCTCCA ACCCAGAGGA GGCACGCACC TGGATCACGG GCCTCAAGTA TCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAG AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAACGGT GATGGCTTAT TGAATATTGA AGAGATTCAC 
CAGCTGATGC ATAAACTAAA TGTCAATCTG CCCCGCAGAA AAGTCAGGCA AATGTTTCAG 
GAAGCAGATA CAGATGAGAA TCAGGGAACC TTGACATTTG AAGAGTTCTG CGTTTTTTAC 
AAAATGATGT CATTGAGACG AGACCTCTAT TTGCTGCTCT TGAGCTACAG TGACAAGAAA 
GACCACCTGA CTGTGGAGGA GCTGGCTCAG TTTTTGAAAG TGGAACAAAA GATGAGTAAT 
GTGACACTGG ACTATTGTCT TGACATCATA ATGAAGTTTG AAGTTTCTGA AGAAAACAAA 
GTGAAAAACG TCCTTGGTAT AGAAGGCTTC ACGAACTTCA TGCGTAGCCC TGCCTGTGAC 
GTATTTAACC CGTTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT GTGCAACTAC 
TACATTGCTT CATCTCACAA CACATACCTG ACTGGGGATC AGCTCCTTTC TCAATCTAAA 
GTGGATATGT ATGCACGGGT GCTACAAGAG GGTTGTAGAT GCGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTGGTCCAC CATGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCATTCG TGAAGAATGA GTTCCCGGTT 
ATCCTGTCCA TTGAGAACCA CTGCAGCATT CAGCAGCAGA GGAAGATTGC TCAGTACCTG 
AAAGGTATAT TGCAGGACAA ACTGGACCTG TCTTCTGTAG ACACCGGAGA GTGCAGGCAG 
CTTCCAAGCC CTCAGAGTCT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCGTAT 
CACCTTGGGG ATGATGCAGA GGAAGGAGAA GTGTCCGATG AGGACAGTGC AGATGAGATT 
GAAGATGAGT GCAAGTTCAA GCTGCATTAC AGTAACGGGA CCACTGAGCA CCAGGTAGAA 
TCTTTCATAC GGAAAAAGCT GGAGTCACTG TTGAAGGAGT CTCAGATTCG AGACAAAGAA 
GATCCAGACA GTTTCACAGT GAGGGCGTTA CTCAAGGCTA CACATGAAGG CTTAAATGCA 
CACCTGAAGC AGAACCTGGA TGTAAAGGAA AGTGGAAAGA AGTCCCATGG GCGATCCCTG 
ATGGCCAACT TTGGGAAACA CAAGAAAGCT ACGAAATCAC GTTCTAAATC CTATAGTACT 
GATGATGAGG ATGATAGCCT TCAGAATCCT GGCAAGGAGG GAGGCCAGCT GTACAGGCTG 
GGCCGCCGAA GGAGAACCAT GAAACTCTGC AGAGAGCTCT CGGACTTGGT GGTGTACACA 
AACTCCGTGG CAGCCCAGGA TATTGTTGAT GATGGAACCA CAGGGAATGT GTTGTCCTTC 
AGCGAAACAA GAGCCCATCA AGTGGTTCAG CAGAAGTCAG AGCAGTTCAT GATCTACAAC 
CAAAAACAGC TCACAAGGAT TTACCCCTCT GCCTACCGCA TCGATTCCAG TAACTTCAAC 
CCACTGCCCT ACTGGAATGC AGGCTGCCAG CTCGTGGCTC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGATCAATCG AGCAAAATTC AAGGCGAATG GCAACTGTGG CTATATTCTC 
AAGCCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC ACTTCCTGCC 
AACCCCAAAA AACAGCTCAT ACTGAAAGTG ATCAGTGGAC AGCAACTCCC CAAACCTCCT 
GACTCTATGT TTGGAGACCG AGGCGAGATC ATTGACCCTT TCGTTGAAGT TGAAATTATT 
GGGCTGCCAG TAGACTGTTG CAAGGACCAA ACTCGTGTGG TGGATGACAA CGGATTTAAC 
CCTGTGTGGG AAGAAACCCT AACTTTTACA GTTCACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCTATTGGA AGAGACTTTG TCGGACAAAG GACTGTGACC 
TTCAGTAGCC TCGTGCCCGG CTACCGGCAT GTCTACCTGG AAGGACTCAC AGAAGCATCT 
ATTTTCGTTC ACATAACCAT CAATGAGATC TTTGGAAAGA ACAGACAGCT CCAAGGCCTG 
AAGGGGCTGT TCAATAAGAA TCCCAGGCAC GCTTCTTCAG AAAACAACTC GCATTATGTT 
CGGAAGCGAT CGATTGGAGA TAGGATTCTG CGACGTACAG CTAGTGCTCC AGCCAAAGGC 
AGGAAAAAGA GCAAAGTGGG CTTCCAAGAA ATGGTTGAGA TAAAAGATTC TGTCTCTGAG 
GCTTCAAGAG ATCAAGATGG TGTCCTGAGG AGGACCACCC GGAGTCTGCA AGTACGCCCT 
GTCTCTATGC CTGTTGACAA GAGCCTTCTG GGGGCTTTGT CGCTGCCCAT ATCTGAAGCA 
GCAAAAGACA CGGATGGAAA AGAAAACTGT CTGGCAGGAG ACAAGGATGA CAGAAGAAAG 
GGAGCCGCAA CTAGAAAAGA CCCACATTTT TCAAATTTCA ACAAAAAGTT ATCCTCCTCC 
TCCAGTGCGC TCCTCCACAA AGATGCCAAC CAAGGGCCAA CTGCCAGTGT ATCAAACCCA 
GAACAGTGTG GAGGACGAGG TGCAAAGAGT GAGAGGATCA AACCAAATAT GACAAATGAT 
TGCCAGGAAA ACCACAATCC CCCGAAATTC CTTTCTCCAA GGAAGCATTT GGCTCTGGAT 
CCTGCAACGA AGGGACTGCA AGAGAGACTG CATGGAATGA AAACCAATGA GAAGGAGCAT 
GCCGAGGGCT TCTTGGGAGA GAAAAGCATG CTGTCCGGAA GCGTTCTTTC TCAAAGTTCT 
CTGGAGGTAG AGAACCTGGA AGGCAGCAGA GCCAAGGGCA GAGCTGCAAC CTCCTTTTCT 
CTCTCGGACG TCTCCGCGCT CTGTTCTGAC ATCCCTGATT TACATTCAAC TGCCATTCTA 
CAGGACACTG AAATTTCCAA TCTCATTGAT GACGTGACTT TAACAAATGA GAATCAGTCG 
GGAAGTTCCA TCTCGGCACT GATTGGCCAG TTTGAAGAAA GCAACCACCC GGCTAACGTT 
ACTGTGGTTT CTCATCTCAG CACCAGTGGG GCATCAGGAA GTGCTCCTTT CCAAACACCC 
TTCAAGCATG GTCTTTCCCA GGGGAACCAG AAGGCCTCCT TCTTGTGCTC ATCTCCTGAG 
CTGAATAAGC TCTCTAGTGT TGAGACCACC AAACTGGCAA ATAACGCAGT TCCTTGTGGA 
GTGATTGGTT CTCCCATCTC TACACCAAAG CCAGGTGATG ACCCTTCAGA TAAGGCCAAG 
ACAAGAGTCA TTGAAGGCAA CCTGCCTGGG TTCCCTGATG CTTCTCCTGG TCAATTTCCC 
AAAAGCCCCA CCCATGGAGA GGACCATAGT CAAGTGATGA ACAGCCCTGC TCTCTCCACG 
GAGTTGGCCA TAGAAGATAT AATTGCAGAC CCTGCTCTCT CTATAAATTC TGCAGAAAGC 
AGTCTTGTGG AAATTGATGG AGAATCTGAA AACCTATCTC TAACAACCTG TGACTACAGG 
GAAGAAGCTC CAAGTCAACT TGTTTCTCCA CTAAAACTGC AGCAGAGCCA GGAGATGGTG 
GAACACATTC AGAGGGGCTT GAGGAATGGC TACTGTAAAG AGACTCTCCT CCCTTCTGAA 
ATATTCAACA ATATTCCAGG TGTCAAAAAT CACAGTATTT CTCATCTAAC CTATCAGGGT 
GCTGGCTTTG TGTATAACCA TTTCTCAAGT TCAGATGCAA AAACGAACCA AATCTGTGAG 
CCCCAGCAGC CTAGGGCTCC AGATATGCAT GCCCCTACAC CTACACCATC AACACATGCT 
CCTTTGGCTG CTTTGAAACT GCCAAGCCCA TGCAAATCCA AAAGTCTGGG GGACTTAACA 
TCAGAGGACA TTGCCTGCAA TTTTGAAAGC AAATATCAAT GTATTAGTAG GAGCTTTGTG 
ACAAACGGCA TTAGGGACAA GAGCGTGACT ATGAAGACAA AGTCATTAGA GCCTTTAGAC 
GCCCTGACTG AACAGCTCCG GAAGCTGGTG TCCTTTGACC AAGAAGACAG CTGTCAAGTG 
CTCTACTCAA AGCAGGATGT CAATCAGTGT CCCAGGGCAT TAGTCAGAAA GTTGTCGTCT 
AGAAGTCAGA GCAGAGTGCG CAACATTGCT AGCCGTGCCA AGGAGAAGCA GGAAGCTGGC 
AAGCAAAAAG CTATGGCCCA GAGCACCAGA GGAGGAGTGG TCCTTAGAAG TAAACCGCCC 
GCTCCTGCTC TGGCCGTGAA CCGTCACTCC ACGGGCTCAT ACATTGCAAG CTACCTGAGG 
AACATGAAAG CTGGTGGCCT AGAGGGTCGA GGCATCCCAG AGGGAGCGTG CACGGCCCTT 
CGCTATGGCT ACATGGACCA GTTTTGTTCA GATAATTCAG TTCTGCAGAC TGAGCCAAGC 
AGCGAAGATA AACCCGAAAT TTATTTTCTT TTGAGGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_011240134.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238436.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu31954
Clone ID Related Accession (Same CDS sequence)	XM_011240134.2 , XM_011240133.2
Accession Version	XM_011240134.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240134.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAA AACTGGAAGC AGATACAGAT GAGAATCAGG GAACCTTGAC ATTTGAAGAG 
TTCTGCGTTT TTTACAAAAT GATGTCATTG AGACGAGACC TCTATTTGCT GCTCTTGAGC 
TACAGTGACA AGAAAGACCA CCTGACTGTG GAGGAGCTGG CTCAGTTTTT GAAAGTGGAA 
CAAAAGATGA GTAATGTGAC ACTGGACTAT TGTCTTGACA TCATAATGAA GTTTGAAGTT 
TCTGAAGAAA ACAAAGTGAA AAACGTCCTT GGTATAGAAG GCTTCACGAA CTTCATGCGT 
AGCCCTGCCT GTGACGTATT TAACCCGTTG CACCATGAAG TGTACCAAGA CATGGATCAG 
CCCCTGTGCA ACTACTACAT TGCTTCATCT CACAACACAT ACCTGACTGG GGATCAGCTC 
CTTTCTCAAT CTAAAGTGGA TATGTATGCA CGGGTGCTAC AAGAGGGTTG TAGATGCGTG 
GAAGTTGACT GTTGGGATGG CCCAGATGGA GAGCCAGTGG TCCACCATGG TTATACTCTC 
ACTTCAAAAA TTCTCTTCAG AGATGTTGTG GAGACCATCA ACAAGCATGC ATTCGTGAAG 
AATGAGTTCC CGGTTATCCT GTCCATTGAG AACCACTGCA GCATTCAGCA GCAGAGGAAG 
ATTGCTCAGT ACCTGAAAGG TATATTGCAG GACAAACTGG ACCTGTCTTC TGTAGACACC 
GGAGAGTGCA GGCAGCTTCC AAGCCCTCAG AGTCTGAAAG GCAAAATCCT AGTGAAGGGC 
AAGAAGTTGC CGTATCACCT TGGGGATGAT GCAGAGGAAG GAGAAGTGTC CGATGAGGAC 
AGTGCAGATG AGATTGAAGA TGAGTGCAAG TTCAAGCTGC ATTACAGTAA CGGGACCACT 
GAGCACCAGG TAGAATCTTT CATACGGAAA AAGCTGGAGT CACTGTTGAA GGAGTCTCAG 
ATTCGAGACA AAGAAGATCC AGACAGTTTC ACAGTGAGGG CGTTACTCAA GGCTACACAT 
GAAGGCTTAA ATGCACACCT GAAGCAGAAC CTGGATGTAA AGGAAAGTGG AAAGAAGTCC 
CATGGGCGAT CCCTGATGGC CAACTTTGGG AAACACAAGC AGAAAGCTAC GAAATCACGT 
TCTAAATCCT ATAGTACTGA TGATGAGGAT GATAGCCTTC AGAATCCTGG CAAGGAGGGA 
GGCCAGCTGT ACAGGCTGGG CCGCCGAAGG AGAACCATGA AACTCTGCAG AGAGCTCTCG 
GACTTGGTGG TGTACACAAA CTCCGTGGCA GCCCAGGATA TTGTTGATGA TGGAACCACA 
GGGAATGTGT TGTCCTTCAG CGAAACAAGA GCCCATCAAG TGGTTCAGCA GAAGTCAGAG 
CAGTTCATGA TCTACAACCA AAAACAGCTC ACAAGGATTT ACCCCTCTGC CTACCGCATC 
GATTCCAGTA ACTTCAACCC ACTGCCCTAC TGGAATGCAG GCTGCCAGCT CGTGGCTCTG 
AACTACCAGT CTGAAGGGCG AATGATGCAG ATCAATCGAG CAAAATTCAA GGCGAATGGC 
AACTGTGGCT ATATTCTCAA GCCCCAGCAA ATGTGCAAAG GTACTTTCAA CCCTTTCTCT 
GGTGACCCAC TTCCTGCCAA CCCCAAAAAA CAGCTCATAC TGAAAGTGAT CAGTGGACAG 
CAACTCCCCA AACCTCCTGA CTCTATGTTT GGAGACCGAG GCGAGATCAT TGACCCTTTC 
GTTGAAGTTG AAATTATTGG GCTGCCAGTA GACTGTTGCA AGGACCAAAC TCGTGTGGTG 
GATGACAACG GATTTAACCC TGTGTGGGAA GAAACCCTAA CTTTTACAGT TCACATGCCA 
GAAATAGCTT TGGTTCGGTT CCTTGTGTGG GATCATGATC CTATTGGAAG AGACTTTGTC 
GGACAAAGGA CTGTGACCTT CAGTAGCCTC GTGCCCGGCT ACCGGCATGT CTACCTGGAA 
GGACTCACAG AAGCATCTAT TTTCGTTCAC ATAACCATCA ATGAGATCTT TGGAAAGTGG 
AGTCCTTTAA TCCTCAACCC CAGTTATACC ATATTGCACT TTCTAGGAGC TACAAAGAAC 
AGACAGCTCC AAGGCCTGAA GGGGCTGTTC AATAAGAATC CCAGGCACGC TTCTTCAGAA 
AACAACTCGC ATTATGTTCG GAAGCGATCG ATTGGAGATA GGATTCTGCG ACGTACAGCT 
AGTGCTCCAG CCAAAGGCAG GAAAAAGAGC AAAGTGGGCT TCCAAGAAAT GGTTGAGATA 
AAAGATTCTG TCTCTGAGGC TTCAAGAGAT CAAGATGGTG TCCTGAGGAG GACCACCCGG 
AGTCTGCAAG TACGCCCTGT CTCTATGCCT GTTGACAAGA GCCTTCTGGG GGCTTTGTCG 
CTGCCCATAT CTGAAGCAGC AAAAGACACG GATGGAAAAG AAAACTGTCT GGCAGGAGAC 
AAGGATGACA GAAGAAAGGG AGCCGCAACT AGAAAAGACC CACATTTTTC AAATTTCAAC 
AAAAAGTTAT CCTCCTCCTC CAGTGCGCTC CTCCACAAAG ATGCCAACCA AGGGCCAACT 
GCCAGTGTAT CAAACCCAGA ACAGTGTGGA GGACGAGGTG CAAAGAGTGA GAGGATCAAA 
CCAAATATGA CAAATGATTG CCAGGAAAAC CACAATCCCC CGAAATTCCT TTCTCCAAGG 
AAGCATTTGG CTCTGGATCC TGCAACGAAG GGACTGCAAG AGAGACTGCA TGGAATGAAA 
ACCAATGAGA AGGAGCATGC CGAGGGCTTC TTGGGAGAGA AAAGCATGCT GTCCGGAAGC 
GTTCTTTCTC AAAGTTCTCT GGAGGTAGAG AACCTGGAAG GCAGCAGAGC CAAGGGCAGA 
GCTGCAACCT CCTTTTCTCT CTCGGACGTC TCCGCGCTCT GTTCTGACAT CCCTGATTTA 
CATTCAACTG CCATTCTACA GGACACTGAA ATTTCCAATC TCATTGATGA CGTGACTTTA 
ACAAATGAGA ATCAGTCGGG AAGTTCCATC TCGGCACTGA TTGGCCAGTT TGAAGAAAGC 
AACCACCCGG CTAACGTTAC TGTGGTTTCT CATCTCAGCA CCAGTGGGGC ATCAGGAAGT 
GCTCCTTTCC AAACACCCTT CAAGCATGGT CTTTCCCAGG GGAACCAGAA GGCCTCCTTC 
TTGTGCTCAT CTCCTGAGCT GAATAAGCTC TCTAGTGTTG AGACCACCAA ACTGGCAAAT 
AACGCAGTTC CTTGTGGAGT GATTGGTTCT CCCATCTCTA CACCAAAGCC AGGTGATGAC 
CCTTCAGATA AGGCCAAGAC AAGAGTCATT GAAGGCAACC TGCCTGGGTT CCCTGATGCT 
TCTCCTGGTC AATTTCCCAA AAGCCCCACC CATGGAGAGG ACCATAGTCA AGTGATGAAC 
AGCCCTGCTC TCTCCACGGA GTTGGCCATA GAAGATATAA TTGCAGACCC TGCTCTCTCT 
ATAAATTCTG CAGAAAGCAG TCTTGTGGAA ATTGATGGAG AATCTGAAAA CCTATCTCTA 
ACAACCTGTG ACTACAGGGA AGAAGCTCCA AGTCAACTTG TTTCTCCACT AAAACTGCAG 
CAGAGCCAGG AGATGGTGGA ACACATTCAG AGGGGCTTGA GGAATGGCTA CTGTAAAGAG 
ACTCTCCTCC CTTCTGAAAT ATTCAACAAT ATTCCAGGTG TCAAAAATCA CAGTATTTCT 
CATCTAACCT ATCAGGGTGC TGGCTTTGTG TATAACCATT TCTCAAGTTC AGATGCAAAA 
ACGAACCAAA TCTGTGAGCC CCAGCAGCCT AGGGCTCCAG ATATGCATGC CCCTACACCT 
ACACCATCAA CACATGCTCC TTTGGCTGCT TTGAAACTGC CAAGCCCATG CAAATCCAAA 
AGTCTGGGGG ACTTAACATC AGAGGACATT GCCTGCAATT TTGAAAGCAA ATATCAATGT 
ATTAGTAGGA GCTTTGTGAC AAACGGCATT AGGGACAAGA GCGTGACTAT GAAGACAAAG 
TCATTAGAGC CTTTAGACGC CCTGACTGAA CAGCTCCGGA AGCTGGTGTC CTTTGACCAA 
GAAGACAGCT GTCAAGTGCT CTACTCAAAG CAGGATGTCA ATCAGTGTCC CAGGGCATTA 
GTCAGAAAGT TGTCGTCTAG AAGTCAGAGC AGAGTGCGCA ACATTGCTAG CCGTGCCAAG 
GAGAAGCAGG AAGCTGGCAA GCAAAAAGCT ATGGCCCAGA GCACCAGAGG AGGAGTGGTC 
CTTAGAAGTA AACCGCCCGC TCCTGCTCTG GCCGTGAACC GTCACTCCAC GGGCTCATAC 
ATTGCAAGCT ACCTGAGGAA CATGAAAGCT GGTGGCCTAG AGGGTCGAGG CATCCCAGAG 
GGAGCGTGCA CGGCCCTTCG CTATGGCTAC ATGGACCAGT TTTGTTCAGA TAATTCAGTT 
CTGCAGACTG AGCCAAGCAG CGAAGATAAA CCCGAAATTT ATTTTCTTTT GAGGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238436.1
CDS	1214..5713
Translation	MKAKLEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_011240134.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240134.2

ORF Insert Sequence:

ATGAAGGCAA AACTGGAAGC AGATACAGAT GAGAATCAGG GAACCTTGAC ATTTGAAGAG 
TTCTGCGTTT TTTACAAAAT GATGTCATTG AGACGAGACC TCTATTTGCT GCTCTTGAGC 
TACAGTGACA AGAAAGACCA CCTGACTGTG GAGGAGCTGG CTCAGTTTTT GAAAGTGGAA 
CAAAAGATGA GTAATGTGAC ACTGGACTAT TGTCTTGACA TCATAATGAA GTTTGAAGTT 
TCTGAAGAAA ACAAAGTGAA AAACGTCCTT GGTATAGAAG GCTTCACGAA CTTCATGCGT 
AGCCCTGCCT GTGACGTATT TAACCCGTTG CACCATGAAG TGTACCAAGA CATGGATCAG 
CCCCTGTGCA ACTACTACAT TGCTTCATCT CACAACACAT ACCTGACTGG GGATCAGCTC 
CTTTCTCAAT CTAAAGTGGA TATGTATGCA CGGGTGCTAC AAGAGGGTTG TAGATGCGTG 
GAAGTTGACT GTTGGGATGG CCCAGATGGA GAGCCAGTGG TCCACCATGG TTATACTCTC 
ACTTCAAAAA TTCTCTTCAG AGATGTTGTG GAGACCATCA ACAAGCATGC ATTCGTGAAG 
AATGAGTTCC CGGTTATCCT GTCCATTGAG AACCACTGCA GCATTCAGCA GCAGAGGAAG 
ATTGCTCAGT ACCTGAAAGG TATATTGCAG GACAAACTGG ACCTGTCTTC TGTAGACACC 
GGAGAGTGCA GGCAGCTTCC AAGCCCTCAG AGTCTGAAAG GCAAAATCCT AGTGAAGGGC 
AAGAAGTTGC CGTATCACCT TGGGGATGAT GCAGAGGAAG GAGAAGTGTC CGATGAGGAC 
AGTGCAGATG AGATTGAAGA TGAGTGCAAG TTCAAGCTGC ATTACAGTAA CGGGACCACT 
GAGCACCAGG TAGAATCTTT CATACGGAAA AAGCTGGAGT CACTGTTGAA GGAGTCTCAG 
ATTCGAGACA AAGAAGATCC AGACAGTTTC ACAGTGAGGG CGTTACTCAA GGCTACACAT 
GAAGGCTTAA ATGCACACCT GAAGCAGAAC CTGGATGTAA AGGAAAGTGG AAAGAAGTCC 
CATGGGCGAT CCCTGATGGC CAACTTTGGG AAACACAAGC AGAAAGCTAC GAAATCACGT 
TCTAAATCCT ATAGTACTGA TGATGAGGAT GATAGCCTTC AGAATCCTGG CAAGGAGGGA 
GGCCAGCTGT ACAGGCTGGG CCGCCGAAGG AGAACCATGA AACTCTGCAG AGAGCTCTCG 
GACTTGGTGG TGTACACAAA CTCCGTGGCA GCCCAGGATA TTGTTGATGA TGGAACCACA 
GGGAATGTGT TGTCCTTCAG CGAAACAAGA GCCCATCAAG TGGTTCAGCA GAAGTCAGAG 
CAGTTCATGA TCTACAACCA AAAACAGCTC ACAAGGATTT ACCCCTCTGC CTACCGCATC 
GATTCCAGTA ACTTCAACCC ACTGCCCTAC TGGAATGCAG GCTGCCAGCT CGTGGCTCTG 
AACTACCAGT CTGAAGGGCG AATGATGCAG ATCAATCGAG CAAAATTCAA GGCGAATGGC 
AACTGTGGCT ATATTCTCAA GCCCCAGCAA ATGTGCAAAG GTACTTTCAA CCCTTTCTCT 
GGTGACCCAC TTCCTGCCAA CCCCAAAAAA CAGCTCATAC TGAAAGTGAT CAGTGGACAG 
CAACTCCCCA AACCTCCTGA CTCTATGTTT GGAGACCGAG GCGAGATCAT TGACCCTTTC 
GTTGAAGTTG AAATTATTGG GCTGCCAGTA GACTGTTGCA AGGACCAAAC TCGTGTGGTG 
GATGACAACG GATTTAACCC TGTGTGGGAA GAAACCCTAA CTTTTACAGT TCACATGCCA 
GAAATAGCTT TGGTTCGGTT CCTTGTGTGG GATCATGATC CTATTGGAAG AGACTTTGTC 
GGACAAAGGA CTGTGACCTT CAGTAGCCTC GTGCCCGGCT ACCGGCATGT CTACCTGGAA 
GGACTCACAG AAGCATCTAT TTTCGTTCAC ATAACCATCA ATGAGATCTT TGGAAAGTGG 
AGTCCTTTAA TCCTCAACCC CAGTTATACC ATATTGCACT TTCTAGGAGC TACAAAGAAC 
AGACAGCTCC AAGGCCTGAA GGGGCTGTTC AATAAGAATC CCAGGCACGC TTCTTCAGAA 
AACAACTCGC ATTATGTTCG GAAGCGATCG ATTGGAGATA GGATTCTGCG ACGTACAGCT 
AGTGCTCCAG CCAAAGGCAG GAAAAAGAGC AAAGTGGGCT TCCAAGAAAT GGTTGAGATA 
AAAGATTCTG TCTCTGAGGC TTCAAGAGAT CAAGATGGTG TCCTGAGGAG GACCACCCGG 
AGTCTGCAAG TACGCCCTGT CTCTATGCCT GTTGACAAGA GCCTTCTGGG GGCTTTGTCG 
CTGCCCATAT CTGAAGCAGC AAAAGACACG GATGGAAAAG AAAACTGTCT GGCAGGAGAC 
AAGGATGACA GAAGAAAGGG AGCCGCAACT AGAAAAGACC CACATTTTTC AAATTTCAAC 
AAAAAGTTAT CCTCCTCCTC CAGTGCGCTC CTCCACAAAG ATGCCAACCA AGGGCCAACT 
GCCAGTGTAT CAAACCCAGA ACAGTGTGGA GGACGAGGTG CAAAGAGTGA GAGGATCAAA 
CCAAATATGA CAAATGATTG CCAGGAAAAC CACAATCCCC CGAAATTCCT TTCTCCAAGG 
AAGCATTTGG CTCTGGATCC TGCAACGAAG GGACTGCAAG AGAGACTGCA TGGAATGAAA 
ACCAATGAGA AGGAGCATGC CGAGGGCTTC TTGGGAGAGA AAAGCATGCT GTCCGGAAGC 
GTTCTTTCTC AAAGTTCTCT GGAGGTAGAG AACCTGGAAG GCAGCAGAGC CAAGGGCAGA 
GCTGCAACCT CCTTTTCTCT CTCGGACGTC TCCGCGCTCT GTTCTGACAT CCCTGATTTA 
CATTCAACTG CCATTCTACA GGACACTGAA ATTTCCAATC TCATTGATGA CGTGACTTTA 
ACAAATGAGA ATCAGTCGGG AAGTTCCATC TCGGCACTGA TTGGCCAGTT TGAAGAAAGC 
AACCACCCGG CTAACGTTAC TGTGGTTTCT CATCTCAGCA CCAGTGGGGC ATCAGGAAGT 
GCTCCTTTCC AAACACCCTT CAAGCATGGT CTTTCCCAGG GGAACCAGAA GGCCTCCTTC 
TTGTGCTCAT CTCCTGAGCT GAATAAGCTC TCTAGTGTTG AGACCACCAA ACTGGCAAAT 
AACGCAGTTC CTTGTGGAGT GATTGGTTCT CCCATCTCTA CACCAAAGCC AGGTGATGAC 
CCTTCAGATA AGGCCAAGAC AAGAGTCATT GAAGGCAACC TGCCTGGGTT CCCTGATGCT 
TCTCCTGGTC AATTTCCCAA AAGCCCCACC CATGGAGAGG ACCATAGTCA AGTGATGAAC 
AGCCCTGCTC TCTCCACGGA GTTGGCCATA GAAGATATAA TTGCAGACCC TGCTCTCTCT 
ATAAATTCTG CAGAAAGCAG TCTTGTGGAA ATTGATGGAG AATCTGAAAA CCTATCTCTA 
ACAACCTGTG ACTACAGGGA AGAAGCTCCA AGTCAACTTG TTTCTCCACT AAAACTGCAG 
CAGAGCCAGG AGATGGTGGA ACACATTCAG AGGGGCTTGA GGAATGGCTA CTGTAAAGAG 
ACTCTCCTCC CTTCTGAAAT ATTCAACAAT ATTCCAGGTG TCAAAAATCA CAGTATTTCT 
CATCTAACCT ATCAGGGTGC TGGCTTTGTG TATAACCATT TCTCAAGTTC AGATGCAAAA 
ACGAACCAAA TCTGTGAGCC CCAGCAGCCT AGGGCTCCAG ATATGCATGC CCCTACACCT 
ACACCATCAA CACATGCTCC TTTGGCTGCT TTGAAACTGC CAAGCCCATG CAAATCCAAA 
AGTCTGGGGG ACTTAACATC AGAGGACATT GCCTGCAATT TTGAAAGCAA ATATCAATGT 
ATTAGTAGGA GCTTTGTGAC AAACGGCATT AGGGACAAGA GCGTGACTAT GAAGACAAAG 
TCATTAGAGC CTTTAGACGC CCTGACTGAA CAGCTCCGGA AGCTGGTGTC CTTTGACCAA 
GAAGACAGCT GTCAAGTGCT CTACTCAAAG CAGGATGTCA ATCAGTGTCC CAGGGCATTA 
GTCAGAAAGT TGTCGTCTAG AAGTCAGAGC AGAGTGCGCA ACATTGCTAG CCGTGCCAAG 
GAGAAGCAGG AAGCTGGCAA GCAAAAAGCT ATGGCCCAGA GCACCAGAGG AGGAGTGGTC 
CTTAGAAGTA AACCGCCCGC TCCTGCTCTG GCCGTGAACC GTCACTCCAC GGGCTCATAC 
ATTGCAAGCT ACCTGAGGAA CATGAAAGCT GGTGGCCTAG AGGGTCGAGG CATCCCAGAG 
GGAGCGTGCA CGGCCCTTCG CTATGGCTAC ATGGACCAGT TTTGTTCAGA TAATTCAGTT 
CTGCAGACTG AGCCAAGCAG CGAAGATAAA CCCGAAATTT ATTTTCTTTT GAGGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch1 ( XM_011240133.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238435.1 Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X12, mRNA.

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CloneID	OMu31954
Clone ID Related Accession (Same CDS sequence)	XM_011240134.2 , XM_011240133.2
Accession Version	XM_011240133.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240133.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAA AACTGGAAGC AGATACAGAT GAGAATCAGG GAACCTTGAC ATTTGAAGAG 
TTCTGCGTTT TTTACAAAAT GATGTCATTG AGACGAGACC TCTATTTGCT GCTCTTGAGC 
TACAGTGACA AGAAAGACCA CCTGACTGTG GAGGAGCTGG CTCAGTTTTT GAAAGTGGAA 
CAAAAGATGA GTAATGTGAC ACTGGACTAT TGTCTTGACA TCATAATGAA GTTTGAAGTT 
TCTGAAGAAA ACAAAGTGAA AAACGTCCTT GGTATAGAAG GCTTCACGAA CTTCATGCGT 
AGCCCTGCCT GTGACGTATT TAACCCGTTG CACCATGAAG TGTACCAAGA CATGGATCAG 
CCCCTGTGCA ACTACTACAT TGCTTCATCT CACAACACAT ACCTGACTGG GGATCAGCTC 
CTTTCTCAAT CTAAAGTGGA TATGTATGCA CGGGTGCTAC AAGAGGGTTG TAGATGCGTG 
GAAGTTGACT GTTGGGATGG CCCAGATGGA GAGCCAGTGG TCCACCATGG TTATACTCTC 
ACTTCAAAAA TTCTCTTCAG AGATGTTGTG GAGACCATCA ACAAGCATGC ATTCGTGAAG 
AATGAGTTCC CGGTTATCCT GTCCATTGAG AACCACTGCA GCATTCAGCA GCAGAGGAAG 
ATTGCTCAGT ACCTGAAAGG TATATTGCAG GACAAACTGG ACCTGTCTTC TGTAGACACC 
GGAGAGTGCA GGCAGCTTCC AAGCCCTCAG AGTCTGAAAG GCAAAATCCT AGTGAAGGGC 
AAGAAGTTGC CGTATCACCT TGGGGATGAT GCAGAGGAAG GAGAAGTGTC CGATGAGGAC 
AGTGCAGATG AGATTGAAGA TGAGTGCAAG TTCAAGCTGC ATTACAGTAA CGGGACCACT 
GAGCACCAGG TAGAATCTTT CATACGGAAA AAGCTGGAGT CACTGTTGAA GGAGTCTCAG 
ATTCGAGACA AAGAAGATCC AGACAGTTTC ACAGTGAGGG CGTTACTCAA GGCTACACAT 
GAAGGCTTAA ATGCACACCT GAAGCAGAAC CTGGATGTAA AGGAAAGTGG AAAGAAGTCC 
CATGGGCGAT CCCTGATGGC CAACTTTGGG AAACACAAGC AGAAAGCTAC GAAATCACGT 
TCTAAATCCT ATAGTACTGA TGATGAGGAT GATAGCCTTC AGAATCCTGG CAAGGAGGGA 
GGCCAGCTGT ACAGGCTGGG CCGCCGAAGG AGAACCATGA AACTCTGCAG AGAGCTCTCG 
GACTTGGTGG TGTACACAAA CTCCGTGGCA GCCCAGGATA TTGTTGATGA TGGAACCACA 
GGGAATGTGT TGTCCTTCAG CGAAACAAGA GCCCATCAAG TGGTTCAGCA GAAGTCAGAG 
CAGTTCATGA TCTACAACCA AAAACAGCTC ACAAGGATTT ACCCCTCTGC CTACCGCATC 
GATTCCAGTA ACTTCAACCC ACTGCCCTAC TGGAATGCAG GCTGCCAGCT CGTGGCTCTG 
AACTACCAGT CTGAAGGGCG AATGATGCAG ATCAATCGAG CAAAATTCAA GGCGAATGGC 
AACTGTGGCT ATATTCTCAA GCCCCAGCAA ATGTGCAAAG GTACTTTCAA CCCTTTCTCT 
GGTGACCCAC TTCCTGCCAA CCCCAAAAAA CAGCTCATAC TGAAAGTGAT CAGTGGACAG 
CAACTCCCCA AACCTCCTGA CTCTATGTTT GGAGACCGAG GCGAGATCAT TGACCCTTTC 
GTTGAAGTTG AAATTATTGG GCTGCCAGTA GACTGTTGCA AGGACCAAAC TCGTGTGGTG 
GATGACAACG GATTTAACCC TGTGTGGGAA GAAACCCTAA CTTTTACAGT TCACATGCCA 
GAAATAGCTT TGGTTCGGTT CCTTGTGTGG GATCATGATC CTATTGGAAG AGACTTTGTC 
GGACAAAGGA CTGTGACCTT CAGTAGCCTC GTGCCCGGCT ACCGGCATGT CTACCTGGAA 
GGACTCACAG AAGCATCTAT TTTCGTTCAC ATAACCATCA ATGAGATCTT TGGAAAGTGG 
AGTCCTTTAA TCCTCAACCC CAGTTATACC ATATTGCACT TTCTAGGAGC TACAAAGAAC 
AGACAGCTCC AAGGCCTGAA GGGGCTGTTC AATAAGAATC CCAGGCACGC TTCTTCAGAA 
AACAACTCGC ATTATGTTCG GAAGCGATCG ATTGGAGATA GGATTCTGCG ACGTACAGCT 
AGTGCTCCAG CCAAAGGCAG GAAAAAGAGC AAAGTGGGCT TCCAAGAAAT GGTTGAGATA 
AAAGATTCTG TCTCTGAGGC TTCAAGAGAT CAAGATGGTG TCCTGAGGAG GACCACCCGG 
AGTCTGCAAG TACGCCCTGT CTCTATGCCT GTTGACAAGA GCCTTCTGGG GGCTTTGTCG 
CTGCCCATAT CTGAAGCAGC AAAAGACACG GATGGAAAAG AAAACTGTCT GGCAGGAGAC 
AAGGATGACA GAAGAAAGGG AGCCGCAACT AGAAAAGACC CACATTTTTC AAATTTCAAC 
AAAAAGTTAT CCTCCTCCTC CAGTGCGCTC CTCCACAAAG ATGCCAACCA AGGGCCAACT 
GCCAGTGTAT CAAACCCAGA ACAGTGTGGA GGACGAGGTG CAAAGAGTGA GAGGATCAAA 
CCAAATATGA CAAATGATTG CCAGGAAAAC CACAATCCCC CGAAATTCCT TTCTCCAAGG 
AAGCATTTGG CTCTGGATCC TGCAACGAAG GGACTGCAAG AGAGACTGCA TGGAATGAAA 
ACCAATGAGA AGGAGCATGC CGAGGGCTTC TTGGGAGAGA AAAGCATGCT GTCCGGAAGC 
GTTCTTTCTC AAAGTTCTCT GGAGGTAGAG AACCTGGAAG GCAGCAGAGC CAAGGGCAGA 
GCTGCAACCT CCTTTTCTCT CTCGGACGTC TCCGCGCTCT GTTCTGACAT CCCTGATTTA 
CATTCAACTG CCATTCTACA GGACACTGAA ATTTCCAATC TCATTGATGA CGTGACTTTA 
ACAAATGAGA ATCAGTCGGG AAGTTCCATC TCGGCACTGA TTGGCCAGTT TGAAGAAAGC 
AACCACCCGG CTAACGTTAC TGTGGTTTCT CATCTCAGCA CCAGTGGGGC ATCAGGAAGT 
GCTCCTTTCC AAACACCCTT CAAGCATGGT CTTTCCCAGG GGAACCAGAA GGCCTCCTTC 
TTGTGCTCAT CTCCTGAGCT GAATAAGCTC TCTAGTGTTG AGACCACCAA ACTGGCAAAT 
AACGCAGTTC CTTGTGGAGT GATTGGTTCT CCCATCTCTA CACCAAAGCC AGGTGATGAC 
CCTTCAGATA AGGCCAAGAC AAGAGTCATT GAAGGCAACC TGCCTGGGTT CCCTGATGCT 
TCTCCTGGTC AATTTCCCAA AAGCCCCACC CATGGAGAGG ACCATAGTCA AGTGATGAAC 
AGCCCTGCTC TCTCCACGGA GTTGGCCATA GAAGATATAA TTGCAGACCC TGCTCTCTCT 
ATAAATTCTG CAGAAAGCAG TCTTGTGGAA ATTGATGGAG AATCTGAAAA CCTATCTCTA 
ACAACCTGTG ACTACAGGGA AGAAGCTCCA AGTCAACTTG TTTCTCCACT AAAACTGCAG 
CAGAGCCAGG AGATGGTGGA ACACATTCAG AGGGGCTTGA GGAATGGCTA CTGTAAAGAG 
ACTCTCCTCC CTTCTGAAAT ATTCAACAAT ATTCCAGGTG TCAAAAATCA CAGTATTTCT 
CATCTAACCT ATCAGGGTGC TGGCTTTGTG TATAACCATT TCTCAAGTTC AGATGCAAAA 
ACGAACCAAA TCTGTGAGCC CCAGCAGCCT AGGGCTCCAG ATATGCATGC CCCTACACCT 
ACACCATCAA CACATGCTCC TTTGGCTGCT TTGAAACTGC CAAGCCCATG CAAATCCAAA 
AGTCTGGGGG ACTTAACATC AGAGGACATT GCCTGCAATT TTGAAAGCAA ATATCAATGT 
ATTAGTAGGA GCTTTGTGAC AAACGGCATT AGGGACAAGA GCGTGACTAT GAAGACAAAG 
TCATTAGAGC CTTTAGACGC CCTGACTGAA CAGCTCCGGA AGCTGGTGTC CTTTGACCAA 
GAAGACAGCT GTCAAGTGCT CTACTCAAAG CAGGATGTCA ATCAGTGTCC CAGGGCATTA 
GTCAGAAAGT TGTCGTCTAG AAGTCAGAGC AGAGTGCGCA ACATTGCTAG CCGTGCCAAG 
GAGAAGCAGG AAGCTGGCAA GCAAAAAGCT ATGGCCCAGA GCACCAGAGG AGGAGTGGTC 
CTTAGAAGTA AACCGCCCGC TCCTGCTCTG GCCGTGAACC GTCACTCCAC GGGCTCATAC 
ATTGCAAGCT ACCTGAGGAA CATGAAAGCT GGTGGCCTAG AGGGTCGAGG CATCCCAGAG 
GGAGCGTGCA CGGCCCTTCG CTATGGCTAC ATGGACCAGT TTTGTTCAGA TAATTCAGTT 
CTGCAGACTG AGCCAAGCAG CGAAGATAAA CCCGAAATTT ATTTTCTTTT GAGGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238435.1
CDS	1226..5725
Translation	MKAKLEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLGALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_011240133.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 1 (Plch1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240133.2

ORF Insert Sequence:

ATGAAGGCAA AACTGGAAGC AGATACAGAT GAGAATCAGG GAACCTTGAC ATTTGAAGAG 
TTCTGCGTTT TTTACAAAAT GATGTCATTG AGACGAGACC TCTATTTGCT GCTCTTGAGC 
TACAGTGACA AGAAAGACCA CCTGACTGTG GAGGAGCTGG CTCAGTTTTT GAAAGTGGAA 
CAAAAGATGA GTAATGTGAC ACTGGACTAT TGTCTTGACA TCATAATGAA GTTTGAAGTT 
TCTGAAGAAA ACAAAGTGAA AAACGTCCTT GGTATAGAAG GCTTCACGAA CTTCATGCGT 
AGCCCTGCCT GTGACGTATT TAACCCGTTG CACCATGAAG TGTACCAAGA CATGGATCAG 
CCCCTGTGCA ACTACTACAT TGCTTCATCT CACAACACAT ACCTGACTGG GGATCAGCTC 
CTTTCTCAAT CTAAAGTGGA TATGTATGCA CGGGTGCTAC AAGAGGGTTG TAGATGCGTG 
GAAGTTGACT GTTGGGATGG CCCAGATGGA GAGCCAGTGG TCCACCATGG TTATACTCTC 
ACTTCAAAAA TTCTCTTCAG AGATGTTGTG GAGACCATCA ACAAGCATGC ATTCGTGAAG 
AATGAGTTCC CGGTTATCCT GTCCATTGAG AACCACTGCA GCATTCAGCA GCAGAGGAAG 
ATTGCTCAGT ACCTGAAAGG TATATTGCAG GACAAACTGG ACCTGTCTTC TGTAGACACC 
GGAGAGTGCA GGCAGCTTCC AAGCCCTCAG AGTCTGAAAG GCAAAATCCT AGTGAAGGGC 
AAGAAGTTGC CGTATCACCT TGGGGATGAT GCAGAGGAAG GAGAAGTGTC CGATGAGGAC 
AGTGCAGATG AGATTGAAGA TGAGTGCAAG TTCAAGCTGC ATTACAGTAA CGGGACCACT 
GAGCACCAGG TAGAATCTTT CATACGGAAA AAGCTGGAGT CACTGTTGAA GGAGTCTCAG 
ATTCGAGACA AAGAAGATCC AGACAGTTTC ACAGTGAGGG CGTTACTCAA GGCTACACAT 
GAAGGCTTAA ATGCACACCT GAAGCAGAAC CTGGATGTAA AGGAAAGTGG AAAGAAGTCC 
CATGGGCGAT CCCTGATGGC CAACTTTGGG AAACACAAGC AGAAAGCTAC GAAATCACGT 
TCTAAATCCT ATAGTACTGA TGATGAGGAT GATAGCCTTC AGAATCCTGG CAAGGAGGGA 
GGCCAGCTGT ACAGGCTGGG CCGCCGAAGG AGAACCATGA AACTCTGCAG AGAGCTCTCG 
GACTTGGTGG TGTACACAAA CTCCGTGGCA GCCCAGGATA TTGTTGATGA TGGAACCACA 
GGGAATGTGT TGTCCTTCAG CGAAACAAGA GCCCATCAAG TGGTTCAGCA GAAGTCAGAG 
CAGTTCATGA TCTACAACCA AAAACAGCTC ACAAGGATTT ACCCCTCTGC CTACCGCATC 
GATTCCAGTA ACTTCAACCC ACTGCCCTAC TGGAATGCAG GCTGCCAGCT CGTGGCTCTG 
AACTACCAGT CTGAAGGGCG AATGATGCAG ATCAATCGAG CAAAATTCAA GGCGAATGGC 
AACTGTGGCT ATATTCTCAA GCCCCAGCAA ATGTGCAAAG GTACTTTCAA CCCTTTCTCT 
GGTGACCCAC TTCCTGCCAA CCCCAAAAAA CAGCTCATAC TGAAAGTGAT CAGTGGACAG 
CAACTCCCCA AACCTCCTGA CTCTATGTTT GGAGACCGAG GCGAGATCAT TGACCCTTTC 
GTTGAAGTTG AAATTATTGG GCTGCCAGTA GACTGTTGCA AGGACCAAAC TCGTGTGGTG 
GATGACAACG GATTTAACCC TGTGTGGGAA GAAACCCTAA CTTTTACAGT TCACATGCCA 
GAAATAGCTT TGGTTCGGTT CCTTGTGTGG GATCATGATC CTATTGGAAG AGACTTTGTC 
GGACAAAGGA CTGTGACCTT CAGTAGCCTC GTGCCCGGCT ACCGGCATGT CTACCTGGAA 
GGACTCACAG AAGCATCTAT TTTCGTTCAC ATAACCATCA ATGAGATCTT TGGAAAGTGG 
AGTCCTTTAA TCCTCAACCC CAGTTATACC ATATTGCACT TTCTAGGAGC TACAAAGAAC 
AGACAGCTCC AAGGCCTGAA GGGGCTGTTC AATAAGAATC CCAGGCACGC TTCTTCAGAA 
AACAACTCGC ATTATGTTCG GAAGCGATCG ATTGGAGATA GGATTCTGCG ACGTACAGCT 
AGTGCTCCAG CCAAAGGCAG GAAAAAGAGC AAAGTGGGCT TCCAAGAAAT GGTTGAGATA 
AAAGATTCTG TCTCTGAGGC TTCAAGAGAT CAAGATGGTG TCCTGAGGAG GACCACCCGG 
AGTCTGCAAG TACGCCCTGT CTCTATGCCT GTTGACAAGA GCCTTCTGGG GGCTTTGTCG 
CTGCCCATAT CTGAAGCAGC AAAAGACACG GATGGAAAAG AAAACTGTCT GGCAGGAGAC 
AAGGATGACA GAAGAAAGGG AGCCGCAACT AGAAAAGACC CACATTTTTC AAATTTCAAC 
AAAAAGTTAT CCTCCTCCTC CAGTGCGCTC CTCCACAAAG ATGCCAACCA AGGGCCAACT 
GCCAGTGTAT CAAACCCAGA ACAGTGTGGA GGACGAGGTG CAAAGAGTGA GAGGATCAAA 
CCAAATATGA CAAATGATTG CCAGGAAAAC CACAATCCCC CGAAATTCCT TTCTCCAAGG 
AAGCATTTGG CTCTGGATCC TGCAACGAAG GGACTGCAAG AGAGACTGCA TGGAATGAAA 
ACCAATGAGA AGGAGCATGC CGAGGGCTTC TTGGGAGAGA AAAGCATGCT GTCCGGAAGC 
GTTCTTTCTC AAAGTTCTCT GGAGGTAGAG AACCTGGAAG GCAGCAGAGC CAAGGGCAGA 
GCTGCAACCT CCTTTTCTCT CTCGGACGTC TCCGCGCTCT GTTCTGACAT CCCTGATTTA 
CATTCAACTG CCATTCTACA GGACACTGAA ATTTCCAATC TCATTGATGA CGTGACTTTA 
ACAAATGAGA ATCAGTCGGG AAGTTCCATC TCGGCACTGA TTGGCCAGTT TGAAGAAAGC 
AACCACCCGG CTAACGTTAC TGTGGTTTCT CATCTCAGCA CCAGTGGGGC ATCAGGAAGT 
GCTCCTTTCC AAACACCCTT CAAGCATGGT CTTTCCCAGG GGAACCAGAA GGCCTCCTTC 
TTGTGCTCAT CTCCTGAGCT GAATAAGCTC TCTAGTGTTG AGACCACCAA ACTGGCAAAT 
AACGCAGTTC CTTGTGGAGT GATTGGTTCT CCCATCTCTA CACCAAAGCC AGGTGATGAC 
CCTTCAGATA AGGCCAAGAC AAGAGTCATT GAAGGCAACC TGCCTGGGTT CCCTGATGCT 
TCTCCTGGTC AATTTCCCAA AAGCCCCACC CATGGAGAGG ACCATAGTCA AGTGATGAAC 
AGCCCTGCTC TCTCCACGGA GTTGGCCATA GAAGATATAA TTGCAGACCC TGCTCTCTCT 
ATAAATTCTG CAGAAAGCAG TCTTGTGGAA ATTGATGGAG AATCTGAAAA CCTATCTCTA 
ACAACCTGTG ACTACAGGGA AGAAGCTCCA AGTCAACTTG TTTCTCCACT AAAACTGCAG 
CAGAGCCAGG AGATGGTGGA ACACATTCAG AGGGGCTTGA GGAATGGCTA CTGTAAAGAG 
ACTCTCCTCC CTTCTGAAAT ATTCAACAAT ATTCCAGGTG TCAAAAATCA CAGTATTTCT 
CATCTAACCT ATCAGGGTGC TGGCTTTGTG TATAACCATT TCTCAAGTTC AGATGCAAAA 
ACGAACCAAA TCTGTGAGCC CCAGCAGCCT AGGGCTCCAG ATATGCATGC CCCTACACCT 
ACACCATCAA CACATGCTCC TTTGGCTGCT TTGAAACTGC CAAGCCCATG CAAATCCAAA 
AGTCTGGGGG ACTTAACATC AGAGGACATT GCCTGCAATT TTGAAAGCAA ATATCAATGT 
ATTAGTAGGA GCTTTGTGAC AAACGGCATT AGGGACAAGA GCGTGACTAT GAAGACAAAG 
TCATTAGAGC CTTTAGACGC CCTGACTGAA CAGCTCCGGA AGCTGGTGTC CTTTGACCAA 
GAAGACAGCT GTCAAGTGCT CTACTCAAAG CAGGATGTCA ATCAGTGTCC CAGGGCATTA 
GTCAGAAAGT TGTCGTCTAG AAGTCAGAGC AGAGTGCGCA ACATTGCTAG CCGTGCCAAG 
GAGAAGCAGG AAGCTGGCAA GCAAAAAGCT ATGGCCCAGA GCACCAGAGG AGGAGTGGTC 
CTTAGAAGTA AACCGCCCGC TCCTGCTCTG GCCGTGAACC GTCACTCCAC GGGCTCATAC 
ATTGCAAGCT ACCTGAGGAA CATGAAAGCT GGTGGCCTAG AGGGTCGAGG CATCCCAGAG 
GGAGCGTGCA CGGCCCTTCG CTATGGCTAC ATGGACCAGT TTTGTTCAGA TAATTCAGTT 
CTGCAGACTG AGCCAAGCAG CGAAGATAAA CCCGAAATTT ATTTTCTTTT GAGGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Phospholipase C? hydrolyzes perinuclear phosphatidylinositol 4-phosphate to regulate cardiac hypertrophy.
Cell153(1)216-27(2013 Mar)
Zhang L,Malik S,Pang J,Wang H,Park KM,Yule DI,Blaxall BC,Smrcka AV

Phospholipase C-?1 is activated by intracellular Ca(2+) mobilization and enhances GPCRs/PLC/Ca(2+) signaling.
Cellular signalling23(6)1022-9(2011 Jun)
Kim JK,Choi JW,Lim S,Kwon O,Seo JK,Ryu SH,Suh PG

Epac2-dependent mobilization of intracellular Ca?+ by glucagon-like peptide-1 receptor agonist exendin-4 is disrupted in ?-cells of phospholipase C-? knockout mice.
The Journal of physiology588(Pt 24)4871-89(2010 Dec)
Dzhura I,Chepurny OG,Kelley GG,Leech CA,Roe MW,Dzhura E,Afshari P,Malik S,Rindler MJ,Xu X,Lu Y,Smrcka AV,Holz GG

Molecular cloning and characterization of a novel phospholipase C, PLC-eta.
The Biochemical journal389(Pt 1)181-6(2005 Jul)
Hwang JI,Oh YS,Shin KJ,Kim H,Ryu SH,Suh PG

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Plch1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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