Nfasc cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nfasc
Entrez Gene ID	269116
Full Name	neurofascin
Synonyms	AA387016,D430023G06Rik,NF,mKIAA0756
General protein information	Preferred Names neurofascin Names neurofascin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 E4\|1 57.42 cM
Summary	This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009].

mRNA and Protein(s)

mRNA	Protein	Name
XM_011248011.1	XP_011246313.1	neurofascin isoform X2
XM_006529639.3	XP_006529702.2	neurofascin isoform X6
XM_011248018.1	XP_011246320.1	neurofascin isoform X15
XM_006529651.2	XP_006529714.2	neurofascin isoform X16
XM_011248020.2	XP_011246322.1	neurofascin isoform X20
XM_011248024.2	XP_011246326.1	neurofascin isoform X19
XM_006529635.4	XP_006529698.2	neurofascin isoform X4
XM_011248016.1	XP_011246318.1	neurofascin isoform X11
XM_006529653.4	XP_006529716.2	neurofascin isoform X18
XM_011248021.1	XP_011246323.1	neurofascin isoform X20
XM_017320903.2	XP_017176392.1	neurofascin isoform X27
XM_030254572.1	XP_030110432.1	neurofascin isoform X31
XM_011248026.2	XP_011246328.1	neurofascin isoform X31
XM_017320909.2	XP_017176398.1	neurofascin isoform X32
XM_017320885.1	XP_017176374.1	neurofascin isoform X13
XM_017320887.1	XP_017176376.1	neurofascin isoform X14
XM_017320897.1	XP_017176386.1	neurofascin isoform X21
XM_017320903.1	XP_017176392.1	neurofascin isoform X24
XM_017320909.1	XP_017176398.1	neurofascin isoform X28
XM_006529634.3	XP_006529697.2	neurofascin isoform X1
XM_011248012.2	XP_011246314.1	neurofascin isoform X3
XM_006529646.3	XP_006529709.2	neurofascin isoform X14
XM_011248018.2	XP_011246320.1	neurofascin isoform X16
XM_006529651.3	XP_006529714.2	neurofascin isoform X17
XM_030254547.1	XP_030110407.1	neurofascin isoform X20
XM_030254549.1	XP_030110409.1	neurofascin isoform X21
XM_011248023.1	XP_011246325.1	neurofascin isoform X19
XM_030254565.1	XP_030110425.1	neurofascin isoform X29
XM_011248012.1	XP_011246314.1	neurofascin isoform X3
XM_006529644.2	XP_006529707.2	neurofascin isoform X10
XM_006529646.2	XP_006529709.2	neurofascin isoform X12
XM_017320907.1	XP_017176396.1	neurofascin isoform X27
XM_017320911.1	XP_017176400.1	neurofascin isoform X29
XM_030254517.1	XP_030110377.1	neurofascin isoform X5
XM_011248013.3	XP_011246315.1	neurofascin isoform X6
XM_011248017.2	XP_011246319.1	neurofascin isoform X13
XM_011248020.3	XP_011246322.1	neurofascin isoform X20
XM_030254557.1	XP_030110417.1	neurofascin isoform X23
XM_030254562.1	XP_030110422.1	neurofascin isoform X28
XM_030254571.1	XP_030110431.1	neurofascin isoform X31
NM_001160318.1	NP_001153790.1	neurofascin isoform 4 precursor
XM_006529634.2	XP_006529697.2	neurofascin isoform X1
XM_011248014.1	XP_011246316.1	neurofascin isoform X7
XM_017320894.1	XP_017176383.1	neurofascin isoform X17
XM_017320912.1	XP_017176401.1	neurofascin isoform X30
XM_011248011.2	XP_011246313.1	neurofascin isoform X2
XM_030254521.1	XP_030110381.1	neurofascin isoform X7
XM_006529639.4	XP_006529702.2	neurofascin isoform X8
XM_011248015.2	XP_011246317.1	neurofascin isoform X10
XM_006529644.3	XP_006529707.2	neurofascin isoform X12
XM_011248019.1	XP_011246321.1	neurofascin isoform X19
XM_017320900.1	XP_017176389.1	neurofascin isoform X24
XM_011248025.3	XP_011246327.1	neurofascin isoform X30
NM_182716.4	NP_874385.1	neurofascin isoform 1 precursor
XM_006529635.3	XP_006529698.2	neurofascin isoform X4
XM_011248013.2	XP_011246315.1	neurofascin isoform X5
XM_011248015.1	XP_011246317.1	neurofascin isoform X8
XM_011248017.1	XP_011246319.1	neurofascin isoform X11
XM_006529653.3	XP_006529716.2	neurofascin isoform X18
XM_011248025.2	XP_011246327.1	neurofascin isoform X25
XM_011248026.1	XP_011246328.1	neurofascin isoform X26
XM_011248014.2	XP_011246316.1	neurofascin isoform X9
XM_030254536.1	XP_030110396.1	neurofascin isoform X15
XM_011248024.3	XP_011246326.1	neurofascin isoform X19
XM_030254551.1	XP_030110411.1	neurofascin isoform X22
XM_017320902.1	XP_017176391.1	neurofascin isoform X25
XM_030254559.1	XP_030110419.1	neurofascin isoform X26
NM_001160317.1	NP_001153789.1	neurofascin isoform 3 precursor
NM_001160316.1	NP_001153788.1	neurofascin isoform 2 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nfasc	NP_874385.1
Xenopus tropicalis (tropical clawed frog)	nfasc	XP_004910755.1
Rattus norvegicus (Norway rat)	Nfasc	NP_001153786.1
Danio rerio (zebrafish)	nfasca	NP_001139239.1
	NFASC	XP_001096628.1
Canis lupus familiaris (dog)	NFASC	XP_005640834.1
Pan troglodytes (chimpanzee)	NFASC	XP_003308755.1
Gallus gallus (chicken)	NFASC	NP_001153794.1
Homo sapiens (human)	NFASC	NP_001005388.2
Bos taurus (cattle)	NFASC	XP_005216764.1

Gene Ontology
Bibliography

Related articles in PubMed

The paranodal cytoskeleton clusters Na
Amor V, Zhang C, Vainshtein A, Zhang A, Zollinger DR, Eshed-Eisenbach Y, Brophy PJ, Rasband MN, Peles E
eLife6(2017 Jan)

Postnatal Loss of Neuronal and Glial Neurofascins Differentially Affects Node of Ranvier Maintenance and Myelinated Axon Function.
Taylor AM, Saifetiarova J, Bhat MA
Frontiers in cellular neuroscience1111(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we identify QKI as the long-sought regulator of Neurofascin alternative splicing
Title: Quaking Regulates Neurofascin 155 Expression for Myelin and Axoglial Junction Maintenance.

Neurofascin 140 is essential for assembly of the node of Ranvier in embryogenesis.
Title: Neurofascin 140 is an embryonic neuronal neurofascin isoform that promotes the assembly of the node of Ranvier.

P0/NFasc Interaction critical for the integrity of paranodal/nodal structures, but not for the stability of myelin
Title: HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.

loss of Nfasc155 was sufficient to cause a robust delay in postnatal synapse elimination at the NMJ across all muscle groups examined.
Title: Loss of glial neurofascin155 delays developmental synapse elimination at the neuromuscular junction.

the apparent dependency on sulfatide for maintaining Nfasc155 and MAG associations is intriguing since the fatty acid composition of sulfatide is altered and paranodal ultrastructure is compromised in multiple sclerosis
Title: Nfasc155H and MAG are specifically susceptible to detergent extraction in the absence of the myelin sphingolipid sulfatide.

The following Nfasc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nfasc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu74388	XM_030254571.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu74388	XM_011248026.2 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu74388	XM_030254572.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu77146	XM_017320907.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu77147	XM_017320909.2 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu74366	XM_006529634.3 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74367	XM_011248011.2 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74368	XM_011248012.2 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74369	XM_006529635.4 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74370	XM_011248013.3 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74371	XM_006529639.4 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74372	XM_011248014.2 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74373	XM_011248015.2 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74375	XM_006529644.3 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu74376	XM_011248017.2 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu74377	XM_006529646.3 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu74379	XM_011248018.2 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu74380	XM_006529651.3 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu74381	XM_006529653.4 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu74382	XM_011248024.3 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74383	XM_030254547.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74383	XM_011248020.3 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74384	XM_030254557.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74385	XM_030254565.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu74387	XM_011248025.3 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu77139	XM_017320885.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu77140	XM_017320887.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu77141	XM_017320894.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu77142	XM_017320897.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu77143	XM_017320900.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu77144	XM_017320902.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu77145	XM_017320903.2 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu77148	XM_017320911.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu77149	XM_017320912.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu85168	XM_030254517.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu85169	XM_030254521.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu85170	XM_030254536.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu85171	XM_030254549.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu85172	XM_030254551.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu85173	XM_030254559.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu85174	XM_030254562.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu22790	NM_001160316.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$699.30 $999.00
OMu22909	NM_001160318.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu22927	NM_182716.4 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$727.30 $1039.00
OMu22945	NM_001160317.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu74374	XM_011248016.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74382	XM_011248023.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74382	XM_011248019.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74383	XM_011248021.1 Latest version!	Mus musculus neurofascin (Nfasc), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu77145	XM_017320903.1	Mus musculus neurofascin (Nfasc), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu77147	XM_017320909.1	Mus musculus neurofascin (Nfasc), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu74379	XM_011248018.1	Mus musculus neurofascin (Nfasc), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu74380	XM_006529651.2	Mus musculus neurofascin (Nfasc), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu74368	XM_011248012.1	Mus musculus neurofascin (Nfasc), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74377	XM_006529646.2	Mus musculus neurofascin (Nfasc), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu74383	XM_011248020.2	Mus musculus neurofascin (Nfasc), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74366	XM_006529634.2	Mus musculus neurofascin (Nfasc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74367	XM_011248011.1	Mus musculus neurofascin (Nfasc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74371	XM_006529639.3	Mus musculus neurofascin (Nfasc), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74375	XM_006529644.2	Mus musculus neurofascin (Nfasc), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu74369	XM_006529635.3	Mus musculus neurofascin (Nfasc), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74370	XM_011248013.2	Mus musculus neurofascin (Nfasc), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74373	XM_011248015.1	Mus musculus neurofascin (Nfasc), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74376	XM_011248017.1	Mus musculus neurofascin (Nfasc), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu74381	XM_006529653.3	Mus musculus neurofascin (Nfasc), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu74387	XM_011248025.2	Mus musculus neurofascin (Nfasc), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu74388	XM_011248026.1	Mus musculus neurofascin (Nfasc), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu74372	XM_011248014.1	Mus musculus neurofascin (Nfasc), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74382	XM_011248024.2	Mus musculus neurofascin (Nfasc), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nfasc ( XM_011248026.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246328.1 Mus musculus neurofascin (Nfasc), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OMu74388
Clone ID Related Accession (Same CDS sequence)	XM_030254572.1 , XM_011248026.2 , XM_030254571.1 , XM_011248026.1
Accession Version	XM_011248026.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG GAGAGCCTTT ACAATCGGCA CCACCCAATC CCAACCGTGA GGTAGCTGGA 
GACACTATCA TCTTCCGGGA TACTCAGATC AGCAGCAGGG CAGTGTACCA ATGTAATACA 
TCCAATGAAC ATGGCTACCT GCTGGCCAAT GCCTTCGTCA GCGTGTTAGA TGTACCCCCT 
CGGATGCTGT CTGCCCGCAA CCAGCTCATC AGGGTGATCC TTTATAACCG GACACGGCTG 
GACTGTCCGT TCTTTGGGTC TCCCATCCCA ACACTCCGAT GGTTTAAGAA TGGGCAAGGA 
AGCAACCTGG ATGGCGGTAA CTACCACGTC TACGAAAACG GCAGTCTAGA AATCAAGATG 
ATTCGCAAAG AGGACCAAGG CATCTACACC TGTGTGGCCA CCAACATCCT GGGCAAAGCC 
GAAAATCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA TCTACAGGAT GCCCGAGGAC 
CAGGTGGCCA AGAGGGGCAC CACGGTGCAG CTGGAGTGCC GCGTGAAACA TGACCCCTCC 
TTGAAGCTCA CAGTCTCCTG GCTGAAGGAC GATGAGCCAC TCTACATTGG AAACAGGATG 
AAGAAGGAAG ATGACTCCCT GACGATCTTC GGAGTGGCAG AGCGGGACCA GGGCAGTTAC 
ACGTGTATGG CCAGCACCGA GCTGGACCAG GACCTGGCAA AGGCCTACCT CACTGTTCTA 
GCTGATCAGG CCACTCCAAC TAACCGTTTG GCTGCCCTAC CCAAAGGGCG ACCAGACCGA 
CCCAGGGACC TGGAGCTCAC TGACCTGGCT GAGAGGAGTG TGAGGCTGAC CTGGATCCCA 
GGGGATGACA ACAACAGCCC TATCACAGAC TACGTCGTTC AGTTTGAAGA GGACCAGTTC 
CAACCAGGGG TGTGGCATGA CCACTCCAGG TTCCCAGGCA GCGTCAACTC AGCCGTCCTC 
CATCTGTCCC CATATGTCAA CTACCAATTC AGAGTCATCG CTGTCAACGA GGTTGGGAGC 
AGCCACCCCA GCCTTCCATC CGAGCGGTAC CGAACCAGTG GGGCACCCCC TGAATCTAAT 
CCCAGTGATG TGAAGGGCGA AGGGACAAGA AAGAACAATA TGGAGATCAC GTGGACGCCT 
ATGAATGCTA CCTCTGCCTT TGGCCCCAAC CTACGCTACA TTGTCAAGTG GCGACGGAGA 
GAGACCCGAG AGACTTGGAA CAATGTCACA GTGTGGGGCT CTCGCTACGT GGTGGGGCAG 
ACGCCTGTCT ACGTTCCCTA TGAGATCCGA GTCCAGGCTG AAAATGACTT TGGGAAAGGC 
CCCGAGCCTG ACACCATCAT TGGGTACTCC GGAGAAGATT ATCCCAGGGC TGCGCCCACT 
GAAGTTAAAA TCCGAGTCCT GAACAGCACA GCCATCAGCC TTCAGTGGAA CCGAGTCTAC 
TCTGACACGG TCCAGGGCCA GCTCAGAGAG TATCGAGCTT ACTACTGGAG GGAAAGCAGT 
TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG AGACAGCAGG CCAGCTTCCC TGGTGACCGT 
CCCCGGGGCG TGGTGGCCCG CCTGTTCCCC TACAGTAACT ACAAGCTGGA GATGGTGGTG 
GTCAATGGGA GAGGTGACGG GCCTCGAAGT GAAACCAAGG AATTCACCAC CCCAGAAGGA 
GTACCCAGTG CCCCCAGGCG GTTCAGAGTC CGACAGCCCA ACCTGGAGAC CATCAACCTG 
GAGTGGGACC ACCCAGAGCA CCCCAACGGA ATCCTGATTG GATACATCCT CAGATACGTG 
CCCTTTAATG GAACCAAACT GGGAAAGCAG ATGGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCTCTG TGCAGAGAGC AGACCCAGTG TCGCGTTACC GCTTCTCCCT CAGTGCCAGG 
ACACAGGTGG GCTCTGGAGA AGCAGCCACA GAGGAGTCCC CAGCACCTCC AAATGAAGCT 
ACTCCAACTG CAGCTCCTCC CACGTTGCCC CCGACTACTG TGGGTACCAC AGGCCTTGTG 
AGCAGTACTG ATGCTACTGC CCTTGCTGCC ACCAGTGAAG CCACAACAGT TCCCATCATT 
CCAACCGTCG TACCTACCAC CGTCGCCACC ACCATTGCCA CAACTACTAC AACCACTGCC 
GCCACCACCA CCACCACCAC TACGGAGAGC CCTCCCACTA CCACTGCTGG GACTAAGATT 
CACGAAACCG CCCCCGACGA GCAGTCCATT TGGAACGTCA CAGTGCTCCC CAACAGTAAA 
TGGGCCAACA TCACCTGGAA GCACAATTTC AGGCCTGGAA CTGACTTTGT GGTTGAGTAC 
ATCGACAGCA ACCATACGAA AAAAACTGTC CCTGTTAAGG CCCAGGCCCA GCCTATACAG 
CTGACAGACC TCTTTCCCGG GATGACGTAC ACGTTGCGGG TGTATTCCCG GGACAACGAG 
GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246328.1
CDS	392..3325
Translation	MVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248026.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248026.1

ORF Insert Sequence:

ATGGTGAATG GAGAGCCTTT ACAATCGGCA CCACCCAATC CCAACCGTGA GGTAGCTGGA 
GACACTATCA TCTTCCGGGA TACTCAGATC AGCAGCAGGG CAGTGTACCA ATGTAATACA 
TCCAATGAAC ATGGCTACCT GCTGGCCAAT GCCTTCGTCA GCGTGTTAGA TGTACCCCCT 
CGGATGCTGT CTGCCCGCAA CCAGCTCATC AGGGTGATCC TTTATAACCG GACACGGCTG 
GACTGTCCGT TCTTTGGGTC TCCCATCCCA ACACTCCGAT GGTTTAAGAA TGGGCAAGGA 
AGCAACCTGG ATGGCGGTAA CTACCACGTC TACGAAAACG GCAGTCTAGA AATCAAGATG 
ATTCGCAAAG AGGACCAAGG CATCTACACC TGTGTGGCCA CCAACATCCT GGGCAAAGCC 
GAAAATCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA TCTACAGGAT GCCCGAGGAC 
CAGGTGGCCA AGAGGGGCAC CACGGTGCAG CTGGAGTGCC GCGTGAAACA TGACCCCTCC 
TTGAAGCTCA CAGTCTCCTG GCTGAAGGAC GATGAGCCAC TCTACATTGG AAACAGGATG 
AAGAAGGAAG ATGACTCCCT GACGATCTTC GGAGTGGCAG AGCGGGACCA GGGCAGTTAC 
ACGTGTATGG CCAGCACCGA GCTGGACCAG GACCTGGCAA AGGCCTACCT CACTGTTCTA 
GCTGATCAGG CCACTCCAAC TAACCGTTTG GCTGCCCTAC CCAAAGGGCG ACCAGACCGA 
CCCAGGGACC TGGAGCTCAC TGACCTGGCT GAGAGGAGTG TGAGGCTGAC CTGGATCCCA 
GGGGATGACA ACAACAGCCC TATCACAGAC TACGTCGTTC AGTTTGAAGA GGACCAGTTC 
CAACCAGGGG TGTGGCATGA CCACTCCAGG TTCCCAGGCA GCGTCAACTC AGCCGTCCTC 
CATCTGTCCC CATATGTCAA CTACCAATTC AGAGTCATCG CTGTCAACGA GGTTGGGAGC 
AGCCACCCCA GCCTTCCATC CGAGCGGTAC CGAACCAGTG GGGCACCCCC TGAATCTAAT 
CCCAGTGATG TGAAGGGCGA AGGGACAAGA AAGAACAATA TGGAGATCAC GTGGACGCCT 
ATGAATGCTA CCTCTGCCTT TGGCCCCAAC CTACGCTACA TTGTCAAGTG GCGACGGAGA 
GAGACCCGAG AGACTTGGAA CAATGTCACA GTGTGGGGCT CTCGCTACGT GGTGGGGCAG 
ACGCCTGTCT ACGTTCCCTA TGAGATCCGA GTCCAGGCTG AAAATGACTT TGGGAAAGGC 
CCCGAGCCTG ACACCATCAT TGGGTACTCC GGAGAAGATT ATCCCAGGGC TGCGCCCACT 
GAAGTTAAAA TCCGAGTCCT GAACAGCACA GCCATCAGCC TTCAGTGGAA CCGAGTCTAC 
TCTGACACGG TCCAGGGCCA GCTCAGAGAG TATCGAGCTT ACTACTGGAG GGAAAGCAGT 
TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG AGACAGCAGG CCAGCTTCCC TGGTGACCGT 
CCCCGGGGCG TGGTGGCCCG CCTGTTCCCC TACAGTAACT ACAAGCTGGA GATGGTGGTG 
GTCAATGGGA GAGGTGACGG GCCTCGAAGT GAAACCAAGG AATTCACCAC CCCAGAAGGA 
GTACCCAGTG CCCCCAGGCG GTTCAGAGTC CGACAGCCCA ACCTGGAGAC CATCAACCTG 
GAGTGGGACC ACCCAGAGCA CCCCAACGGA ATCCTGATTG GATACATCCT CAGATACGTG 
CCCTTTAATG GAACCAAACT GGGAAAGCAG ATGGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCTCTG TGCAGAGAGC AGACCCAGTG TCGCGTTACC GCTTCTCCCT CAGTGCCAGG 
ACACAGGTGG GCTCTGGAGA AGCAGCCACA GAGGAGTCCC CAGCACCTCC AAATGAAGCT 
ACTCCAACTG CAGCTCCTCC CACGTTGCCC CCGACTACTG TGGGTACCAC AGGCCTTGTG 
AGCAGTACTG ATGCTACTGC CCTTGCTGCC ACCAGTGAAG CCACAACAGT TCCCATCATT 
CCAACCGTCG TACCTACCAC CGTCGCCACC ACCATTGCCA CAACTACTAC AACCACTGCC 
GCCACCACCA CCACCACCAC TACGGAGAGC CCTCCCACTA CCACTGCTGG GACTAAGATT 
CACGAAACCG CCCCCGACGA GCAGTCCATT TGGAACGTCA CAGTGCTCCC CAACAGTAAA 
TGGGCCAACA TCACCTGGAA GCACAATTTC AGGCCTGGAA CTGACTTTGT GGTTGAGTAC 
ATCGACAGCA ACCATACGAA AAAAACTGTC CCTGTTAAGG CCCAGGCCCA GCCTATACAG 
CTGACAGACC TCTTTCCCGG GATGACGTAC ACGTTGCGGG TGTATTCCCG GGACAACGAG 
GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_030254572.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110432.1 Mus musculus neurofascin (Nfasc), transcript variant X38, mRNA.

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CloneID	OMu74388
Clone ID Related Accession (Same CDS sequence)	XM_030254572.1 , XM_011248026.2 , XM_030254571.1 , XM_011248026.1
Accession Version	XM_030254572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG GAGAGCCTTT ACAATCGGCA CCACCCAATC CCAACCGTGA GGTAGCTGGA 
GACACTATCA TCTTCCGGGA TACTCAGATC AGCAGCAGGG CAGTGTACCA ATGTAATACA 
TCCAATGAAC ATGGCTACCT GCTGGCCAAT GCCTTCGTCA GCGTGTTAGA TGTACCCCCT 
CGGATGCTGT CTGCCCGCAA CCAGCTCATC AGGGTGATCC TTTATAACCG GACACGGCTG 
GACTGTCCGT TCTTTGGGTC TCCCATCCCA ACACTCCGAT GGTTTAAGAA TGGGCAAGGA 
AGCAACCTGG ATGGCGGTAA CTACCACGTC TACGAAAACG GCAGTCTAGA AATCAAGATG 
ATTCGCAAAG AGGACCAAGG CATCTACACC TGTGTGGCCA CCAACATCCT GGGCAAAGCC 
GAAAATCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA TCTACAGGAT GCCCGAGGAC 
CAGGTGGCCA AGAGGGGCAC CACGGTGCAG CTGGAGTGCC GCGTGAAACA TGACCCCTCC 
TTGAAGCTCA CAGTCTCCTG GCTGAAGGAC GATGAGCCAC TCTACATTGG AAACAGGATG 
AAGAAGGAAG ATGACTCCCT GACGATCTTC GGAGTGGCAG AGCGGGACCA GGGCAGTTAC 
ACGTGTATGG CCAGCACCGA GCTGGACCAG GACCTGGCAA AGGCCTACCT CACTGTTCTA 
GCTGATCAGG CCACTCCAAC TAACCGTTTG GCTGCCCTAC CCAAAGGGCG ACCAGACCGA 
CCCAGGGACC TGGAGCTCAC TGACCTGGCT GAGAGGAGTG TGAGGCTGAC CTGGATCCCA 
GGGGATGACA ACAACAGCCC TATCACAGAC TACGTCGTTC AGTTTGAAGA GGACCAGTTC 
CAACCAGGGG TGTGGCATGA CCACTCCAGG TTCCCAGGCA GCGTCAACTC AGCCGTCCTC 
CATCTGTCCC CATATGTCAA CTACCAATTC AGAGTCATCG CTGTCAACGA GGTTGGGAGC 
AGCCACCCCA GCCTTCCATC CGAGCGGTAC CGAACCAGTG GGGCACCCCC TGAATCTAAT 
CCCAGTGATG TGAAGGGCGA AGGGACAAGA AAGAACAATA TGGAGATCAC GTGGACGCCT 
ATGAATGCTA CCTCTGCCTT TGGCCCCAAC CTACGCTACA TTGTCAAGTG GCGACGGAGA 
GAGACCCGAG AGACTTGGAA CAATGTCACA GTGTGGGGCT CTCGCTACGT GGTGGGGCAG 
ACGCCTGTCT ACGTTCCCTA TGAGATCCGA GTCCAGGCTG AAAATGACTT TGGGAAAGGC 
CCCGAGCCTG ACACCATCAT TGGGTACTCC GGAGAAGATT ATCCCAGGGC TGCGCCCACT 
GAAGTTAAAA TCCGAGTCCT GAACAGCACA GCCATCAGCC TTCAGTGGAA CCGAGTCTAC 
TCTGACACGG TCCAGGGCCA GCTCAGAGAG TATCGAGCTT ACTACTGGAG GGAAAGCAGT 
TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG AGACAGCAGG CCAGCTTCCC TGGTGACCGT 
CCCCGGGGCG TGGTGGCCCG CCTGTTCCCC TACAGTAACT ACAAGCTGGA GATGGTGGTG 
GTCAATGGGA GAGGTGACGG GCCTCGAAGT GAAACCAAGG AATTCACCAC CCCAGAAGGA 
GTACCCAGTG CCCCCAGGCG GTTCAGAGTC CGACAGCCCA ACCTGGAGAC CATCAACCTG 
GAGTGGGACC ACCCAGAGCA CCCCAACGGA ATCCTGATTG GATACATCCT CAGATACGTG 
CCCTTTAATG GAACCAAACT GGGAAAGCAG ATGGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCTCTG TGCAGAGAGC AGACCCAGTG TCGCGTTACC GCTTCTCCCT CAGTGCCAGG 
ACACAGGTGG GCTCTGGAGA AGCAGCCACA GAGGAGTCCC CAGCACCTCC AAATGAAGCT 
ACTCCAACTG CAGCTCCTCC CACGTTGCCC CCGACTACTG TGGGTACCAC AGGCCTTGTG 
AGCAGTACTG ATGCTACTGC CCTTGCTGCC ACCAGTGAAG CCACAACAGT TCCCATCATT 
CCAACCGTCG TACCTACCAC CGTCGCCACC ACCATTGCCA CAACTACTAC AACCACTGCC 
GCCACCACCA CCACCACCAC TACGGAGAGC CCTCCCACTA CCACTGCTGG GACTAAGATT 
CACGAAACCG CCCCCGACGA GCAGTCCATT TGGAACGTCA CAGTGCTCCC CAACAGTAAA 
TGGGCCAACA TCACCTGGAA GCACAATTTC AGGCCTGGAA CTGACTTTGT GGTTGAGTAC 
ATCGACAGCA ACCATACGAA AAAAACTGTC CCTGTTAAGG CCCAGGCCCA GCCTATACAG 
CTGACAGACC TCTTTCCCGG GATGACGTAC ACGTTGCGGG TGTATTCCCG GGACAACGAG 
GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110432.1
CDS	1422..4355
Translation	MVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_030254572.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254572.1

ORF Insert Sequence:

ATGGTGAATG GAGAGCCTTT ACAATCGGCA CCACCCAATC CCAACCGTGA GGTAGCTGGA 
GACACTATCA TCTTCCGGGA TACTCAGATC AGCAGCAGGG CAGTGTACCA ATGTAATACA 
TCCAATGAAC ATGGCTACCT GCTGGCCAAT GCCTTCGTCA GCGTGTTAGA TGTACCCCCT 
CGGATGCTGT CTGCCCGCAA CCAGCTCATC AGGGTGATCC TTTATAACCG GACACGGCTG 
GACTGTCCGT TCTTTGGGTC TCCCATCCCA ACACTCCGAT GGTTTAAGAA TGGGCAAGGA 
AGCAACCTGG ATGGCGGTAA CTACCACGTC TACGAAAACG GCAGTCTAGA AATCAAGATG 
ATTCGCAAAG AGGACCAAGG CATCTACACC TGTGTGGCCA CCAACATCCT GGGCAAAGCC 
GAAAATCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA TCTACAGGAT GCCCGAGGAC 
CAGGTGGCCA AGAGGGGCAC CACGGTGCAG CTGGAGTGCC GCGTGAAACA TGACCCCTCC 
TTGAAGCTCA CAGTCTCCTG GCTGAAGGAC GATGAGCCAC TCTACATTGG AAACAGGATG 
AAGAAGGAAG ATGACTCCCT GACGATCTTC GGAGTGGCAG AGCGGGACCA GGGCAGTTAC 
ACGTGTATGG CCAGCACCGA GCTGGACCAG GACCTGGCAA AGGCCTACCT CACTGTTCTA 
GCTGATCAGG CCACTCCAAC TAACCGTTTG GCTGCCCTAC CCAAAGGGCG ACCAGACCGA 
CCCAGGGACC TGGAGCTCAC TGACCTGGCT GAGAGGAGTG TGAGGCTGAC CTGGATCCCA 
GGGGATGACA ACAACAGCCC TATCACAGAC TACGTCGTTC AGTTTGAAGA GGACCAGTTC 
CAACCAGGGG TGTGGCATGA CCACTCCAGG TTCCCAGGCA GCGTCAACTC AGCCGTCCTC 
CATCTGTCCC CATATGTCAA CTACCAATTC AGAGTCATCG CTGTCAACGA GGTTGGGAGC 
AGCCACCCCA GCCTTCCATC CGAGCGGTAC CGAACCAGTG GGGCACCCCC TGAATCTAAT 
CCCAGTGATG TGAAGGGCGA AGGGACAAGA AAGAACAATA TGGAGATCAC GTGGACGCCT 
ATGAATGCTA CCTCTGCCTT TGGCCCCAAC CTACGCTACA TTGTCAAGTG GCGACGGAGA 
GAGACCCGAG AGACTTGGAA CAATGTCACA GTGTGGGGCT CTCGCTACGT GGTGGGGCAG 
ACGCCTGTCT ACGTTCCCTA TGAGATCCGA GTCCAGGCTG AAAATGACTT TGGGAAAGGC 
CCCGAGCCTG ACACCATCAT TGGGTACTCC GGAGAAGATT ATCCCAGGGC TGCGCCCACT 
GAAGTTAAAA TCCGAGTCCT GAACAGCACA GCCATCAGCC TTCAGTGGAA CCGAGTCTAC 
TCTGACACGG TCCAGGGCCA GCTCAGAGAG TATCGAGCTT ACTACTGGAG GGAAAGCAGT 
TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG AGACAGCAGG CCAGCTTCCC TGGTGACCGT 
CCCCGGGGCG TGGTGGCCCG CCTGTTCCCC TACAGTAACT ACAAGCTGGA GATGGTGGTG 
GTCAATGGGA GAGGTGACGG GCCTCGAAGT GAAACCAAGG AATTCACCAC CCCAGAAGGA 
GTACCCAGTG CCCCCAGGCG GTTCAGAGTC CGACAGCCCA ACCTGGAGAC CATCAACCTG 
GAGTGGGACC ACCCAGAGCA CCCCAACGGA ATCCTGATTG GATACATCCT CAGATACGTG 
CCCTTTAATG GAACCAAACT GGGAAAGCAG ATGGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCTCTG TGCAGAGAGC AGACCCAGTG TCGCGTTACC GCTTCTCCCT CAGTGCCAGG 
ACACAGGTGG GCTCTGGAGA AGCAGCCACA GAGGAGTCCC CAGCACCTCC AAATGAAGCT 
ACTCCAACTG CAGCTCCTCC CACGTTGCCC CCGACTACTG TGGGTACCAC AGGCCTTGTG 
AGCAGTACTG ATGCTACTGC CCTTGCTGCC ACCAGTGAAG CCACAACAGT TCCCATCATT 
CCAACCGTCG TACCTACCAC CGTCGCCACC ACCATTGCCA CAACTACTAC AACCACTGCC 
GCCACCACCA CCACCACCAC TACGGAGAGC CCTCCCACTA CCACTGCTGG GACTAAGATT 
CACGAAACCG CCCCCGACGA GCAGTCCATT TGGAACGTCA CAGTGCTCCC CAACAGTAAA 
TGGGCCAACA TCACCTGGAA GCACAATTTC AGGCCTGGAA CTGACTTTGT GGTTGAGTAC 
ATCGACAGCA ACCATACGAA AAAAACTGTC CCTGTTAAGG CCCAGGCCCA GCCTATACAG 
CTGACAGACC TCTTTCCCGG GATGACGTAC ACGTTGCGGG TGTATTCCCG GGACAACGAG 
GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248026.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246328.1 Mus musculus neurofascin (Nfasc), transcript variant X39, mRNA.

Price & Availability↑

CloneID	OMu74388
Clone ID Related Accession (Same CDS sequence)	XM_030254572.1 , XM_011248026.2 , XM_030254571.1 , XM_011248026.1
Accession Version	XM_011248026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248026.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG GAGAGCCTTT ACAATCGGCA CCACCCAATC CCAACCGTGA GGTAGCTGGA 
GACACTATCA TCTTCCGGGA TACTCAGATC AGCAGCAGGG CAGTGTACCA ATGTAATACA 
TCCAATGAAC ATGGCTACCT GCTGGCCAAT GCCTTCGTCA GCGTGTTAGA TGTACCCCCT 
CGGATGCTGT CTGCCCGCAA CCAGCTCATC AGGGTGATCC TTTATAACCG GACACGGCTG 
GACTGTCCGT TCTTTGGGTC TCCCATCCCA ACACTCCGAT GGTTTAAGAA TGGGCAAGGA 
AGCAACCTGG ATGGCGGTAA CTACCACGTC TACGAAAACG GCAGTCTAGA AATCAAGATG 
ATTCGCAAAG AGGACCAAGG CATCTACACC TGTGTGGCCA CCAACATCCT GGGCAAAGCC 
GAAAATCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA TCTACAGGAT GCCCGAGGAC 
CAGGTGGCCA AGAGGGGCAC CACGGTGCAG CTGGAGTGCC GCGTGAAACA TGACCCCTCC 
TTGAAGCTCA CAGTCTCCTG GCTGAAGGAC GATGAGCCAC TCTACATTGG AAACAGGATG 
AAGAAGGAAG ATGACTCCCT GACGATCTTC GGAGTGGCAG AGCGGGACCA GGGCAGTTAC 
ACGTGTATGG CCAGCACCGA GCTGGACCAG GACCTGGCAA AGGCCTACCT CACTGTTCTA 
GCTGATCAGG CCACTCCAAC TAACCGTTTG GCTGCCCTAC CCAAAGGGCG ACCAGACCGA 
CCCAGGGACC TGGAGCTCAC TGACCTGGCT GAGAGGAGTG TGAGGCTGAC CTGGATCCCA 
GGGGATGACA ACAACAGCCC TATCACAGAC TACGTCGTTC AGTTTGAAGA GGACCAGTTC 
CAACCAGGGG TGTGGCATGA CCACTCCAGG TTCCCAGGCA GCGTCAACTC AGCCGTCCTC 
CATCTGTCCC CATATGTCAA CTACCAATTC AGAGTCATCG CTGTCAACGA GGTTGGGAGC 
AGCCACCCCA GCCTTCCATC CGAGCGGTAC CGAACCAGTG GGGCACCCCC TGAATCTAAT 
CCCAGTGATG TGAAGGGCGA AGGGACAAGA AAGAACAATA TGGAGATCAC GTGGACGCCT 
ATGAATGCTA CCTCTGCCTT TGGCCCCAAC CTACGCTACA TTGTCAAGTG GCGACGGAGA 
GAGACCCGAG AGACTTGGAA CAATGTCACA GTGTGGGGCT CTCGCTACGT GGTGGGGCAG 
ACGCCTGTCT ACGTTCCCTA TGAGATCCGA GTCCAGGCTG AAAATGACTT TGGGAAAGGC 
CCCGAGCCTG ACACCATCAT TGGGTACTCC GGAGAAGATT ATCCCAGGGC TGCGCCCACT 
GAAGTTAAAA TCCGAGTCCT GAACAGCACA GCCATCAGCC TTCAGTGGAA CCGAGTCTAC 
TCTGACACGG TCCAGGGCCA GCTCAGAGAG TATCGAGCTT ACTACTGGAG GGAAAGCAGT 
TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG AGACAGCAGG CCAGCTTCCC TGGTGACCGT 
CCCCGGGGCG TGGTGGCCCG CCTGTTCCCC TACAGTAACT ACAAGCTGGA GATGGTGGTG 
GTCAATGGGA GAGGTGACGG GCCTCGAAGT GAAACCAAGG AATTCACCAC CCCAGAAGGA 
GTACCCAGTG CCCCCAGGCG GTTCAGAGTC CGACAGCCCA ACCTGGAGAC CATCAACCTG 
GAGTGGGACC ACCCAGAGCA CCCCAACGGA ATCCTGATTG GATACATCCT CAGATACGTG 
CCCTTTAATG GAACCAAACT GGGAAAGCAG ATGGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCTCTG TGCAGAGAGC AGACCCAGTG TCGCGTTACC GCTTCTCCCT CAGTGCCAGG 
ACACAGGTGG GCTCTGGAGA AGCAGCCACA GAGGAGTCCC CAGCACCTCC AAATGAAGCT 
ACTCCAACTG CAGCTCCTCC CACGTTGCCC CCGACTACTG TGGGTACCAC AGGCCTTGTG 
AGCAGTACTG ATGCTACTGC CCTTGCTGCC ACCAGTGAAG CCACAACAGT TCCCATCATT 
CCAACCGTCG TACCTACCAC CGTCGCCACC ACCATTGCCA CAACTACTAC AACCACTGCC 
GCCACCACCA CCACCACCAC TACGGAGAGC CCTCCCACTA CCACTGCTGG GACTAAGATT 
CACGAAACCG CCCCCGACGA GCAGTCCATT TGGAACGTCA CAGTGCTCCC CAACAGTAAA 
TGGGCCAACA TCACCTGGAA GCACAATTTC AGGCCTGGAA CTGACTTTGT GGTTGAGTAC 
ATCGACAGCA ACCATACGAA AAAAACTGTC CCTGTTAAGG CCCAGGCCCA GCCTATACAG 
CTGACAGACC TCTTTCCCGG GATGACGTAC ACGTTGCGGG TGTATTCCCG GGACAACGAG 
GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246328.1
CDS	1995..4928
Translation	MVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248026.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248026.2

ORF Insert Sequence:

ATGGTGAATG GAGAGCCTTT ACAATCGGCA CCACCCAATC CCAACCGTGA GGTAGCTGGA 
GACACTATCA TCTTCCGGGA TACTCAGATC AGCAGCAGGG CAGTGTACCA ATGTAATACA 
TCCAATGAAC ATGGCTACCT GCTGGCCAAT GCCTTCGTCA GCGTGTTAGA TGTACCCCCT 
CGGATGCTGT CTGCCCGCAA CCAGCTCATC AGGGTGATCC TTTATAACCG GACACGGCTG 
GACTGTCCGT TCTTTGGGTC TCCCATCCCA ACACTCCGAT GGTTTAAGAA TGGGCAAGGA 
AGCAACCTGG ATGGCGGTAA CTACCACGTC TACGAAAACG GCAGTCTAGA AATCAAGATG 
ATTCGCAAAG AGGACCAAGG CATCTACACC TGTGTGGCCA CCAACATCCT GGGCAAAGCC 
GAAAATCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA TCTACAGGAT GCCCGAGGAC 
CAGGTGGCCA AGAGGGGCAC CACGGTGCAG CTGGAGTGCC GCGTGAAACA TGACCCCTCC 
TTGAAGCTCA CAGTCTCCTG GCTGAAGGAC GATGAGCCAC TCTACATTGG AAACAGGATG 
AAGAAGGAAG ATGACTCCCT GACGATCTTC GGAGTGGCAG AGCGGGACCA GGGCAGTTAC 
ACGTGTATGG CCAGCACCGA GCTGGACCAG GACCTGGCAA AGGCCTACCT CACTGTTCTA 
GCTGATCAGG CCACTCCAAC TAACCGTTTG GCTGCCCTAC CCAAAGGGCG ACCAGACCGA 
CCCAGGGACC TGGAGCTCAC TGACCTGGCT GAGAGGAGTG TGAGGCTGAC CTGGATCCCA 
GGGGATGACA ACAACAGCCC TATCACAGAC TACGTCGTTC AGTTTGAAGA GGACCAGTTC 
CAACCAGGGG TGTGGCATGA CCACTCCAGG TTCCCAGGCA GCGTCAACTC AGCCGTCCTC 
CATCTGTCCC CATATGTCAA CTACCAATTC AGAGTCATCG CTGTCAACGA GGTTGGGAGC 
AGCCACCCCA GCCTTCCATC CGAGCGGTAC CGAACCAGTG GGGCACCCCC TGAATCTAAT 
CCCAGTGATG TGAAGGGCGA AGGGACAAGA AAGAACAATA TGGAGATCAC GTGGACGCCT 
ATGAATGCTA CCTCTGCCTT TGGCCCCAAC CTACGCTACA TTGTCAAGTG GCGACGGAGA 
GAGACCCGAG AGACTTGGAA CAATGTCACA GTGTGGGGCT CTCGCTACGT GGTGGGGCAG 
ACGCCTGTCT ACGTTCCCTA TGAGATCCGA GTCCAGGCTG AAAATGACTT TGGGAAAGGC 
CCCGAGCCTG ACACCATCAT TGGGTACTCC GGAGAAGATT ATCCCAGGGC TGCGCCCACT 
GAAGTTAAAA TCCGAGTCCT GAACAGCACA GCCATCAGCC TTCAGTGGAA CCGAGTCTAC 
TCTGACACGG TCCAGGGCCA GCTCAGAGAG TATCGAGCTT ACTACTGGAG GGAAAGCAGT 
TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG AGACAGCAGG CCAGCTTCCC TGGTGACCGT 
CCCCGGGGCG TGGTGGCCCG CCTGTTCCCC TACAGTAACT ACAAGCTGGA GATGGTGGTG 
GTCAATGGGA GAGGTGACGG GCCTCGAAGT GAAACCAAGG AATTCACCAC CCCAGAAGGA 
GTACCCAGTG CCCCCAGGCG GTTCAGAGTC CGACAGCCCA ACCTGGAGAC CATCAACCTG 
GAGTGGGACC ACCCAGAGCA CCCCAACGGA ATCCTGATTG GATACATCCT CAGATACGTG 
CCCTTTAATG GAACCAAACT GGGAAAGCAG ATGGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCTCTG TGCAGAGAGC AGACCCAGTG TCGCGTTACC GCTTCTCCCT CAGTGCCAGG 
ACACAGGTGG GCTCTGGAGA AGCAGCCACA GAGGAGTCCC CAGCACCTCC AAATGAAGCT 
ACTCCAACTG CAGCTCCTCC CACGTTGCCC CCGACTACTG TGGGTACCAC AGGCCTTGTG 
AGCAGTACTG ATGCTACTGC CCTTGCTGCC ACCAGTGAAG CCACAACAGT TCCCATCATT 
CCAACCGTCG TACCTACCAC CGTCGCCACC ACCATTGCCA CAACTACTAC AACCACTGCC 
GCCACCACCA CCACCACCAC TACGGAGAGC CCTCCCACTA CCACTGCTGG GACTAAGATT 
CACGAAACCG CCCCCGACGA GCAGTCCATT TGGAACGTCA CAGTGCTCCC CAACAGTAAA 
TGGGCCAACA TCACCTGGAA GCACAATTTC AGGCCTGGAA CTGACTTTGT GGTTGAGTAC 
ATCGACAGCA ACCATACGAA AAAAACTGTC CCTGTTAAGG CCCAGGCCCA GCCTATACAG 
CTGACAGACC TCTTTCCCGG GATGACGTAC ACGTTGCGGG TGTATTCCCG GGACAACGAG 
GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_030254571.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110431.1 Mus musculus neurofascin (Nfasc), transcript variant X37, mRNA.

Price & Availability↑

CloneID	OMu74388
Clone ID Related Accession (Same CDS sequence)	XM_030254572.1 , XM_011248026.2 , XM_030254571.1 , XM_011248026.1
Accession Version	XM_030254571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATG GAGAGCCTTT ACAATCGGCA CCACCCAATC CCAACCGTGA GGTAGCTGGA 
GACACTATCA TCTTCCGGGA TACTCAGATC AGCAGCAGGG CAGTGTACCA ATGTAATACA 
TCCAATGAAC ATGGCTACCT GCTGGCCAAT GCCTTCGTCA GCGTGTTAGA TGTACCCCCT 
CGGATGCTGT CTGCCCGCAA CCAGCTCATC AGGGTGATCC TTTATAACCG GACACGGCTG 
GACTGTCCGT TCTTTGGGTC TCCCATCCCA ACACTCCGAT GGTTTAAGAA TGGGCAAGGA 
AGCAACCTGG ATGGCGGTAA CTACCACGTC TACGAAAACG GCAGTCTAGA AATCAAGATG 
ATTCGCAAAG AGGACCAAGG CATCTACACC TGTGTGGCCA CCAACATCCT GGGCAAAGCC 
GAAAATCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA TCTACAGGAT GCCCGAGGAC 
CAGGTGGCCA AGAGGGGCAC CACGGTGCAG CTGGAGTGCC GCGTGAAACA TGACCCCTCC 
TTGAAGCTCA CAGTCTCCTG GCTGAAGGAC GATGAGCCAC TCTACATTGG AAACAGGATG 
AAGAAGGAAG ATGACTCCCT GACGATCTTC GGAGTGGCAG AGCGGGACCA GGGCAGTTAC 
ACGTGTATGG CCAGCACCGA GCTGGACCAG GACCTGGCAA AGGCCTACCT CACTGTTCTA 
GCTGATCAGG CCACTCCAAC TAACCGTTTG GCTGCCCTAC CCAAAGGGCG ACCAGACCGA 
CCCAGGGACC TGGAGCTCAC TGACCTGGCT GAGAGGAGTG TGAGGCTGAC CTGGATCCCA 
GGGGATGACA ACAACAGCCC TATCACAGAC TACGTCGTTC AGTTTGAAGA GGACCAGTTC 
CAACCAGGGG TGTGGCATGA CCACTCCAGG TTCCCAGGCA GCGTCAACTC AGCCGTCCTC 
CATCTGTCCC CATATGTCAA CTACCAATTC AGAGTCATCG CTGTCAACGA GGTTGGGAGC 
AGCCACCCCA GCCTTCCATC CGAGCGGTAC CGAACCAGTG GGGCACCCCC TGAATCTAAT 
CCCAGTGATG TGAAGGGCGA AGGGACAAGA AAGAACAATA TGGAGATCAC GTGGACGCCT 
ATGAATGCTA CCTCTGCCTT TGGCCCCAAC CTACGCTACA TTGTCAAGTG GCGACGGAGA 
GAGACCCGAG AGACTTGGAA CAATGTCACA GTGTGGGGCT CTCGCTACGT GGTGGGGCAG 
ACGCCTGTCT ACGTTCCCTA TGAGATCCGA GTCCAGGCTG AAAATGACTT TGGGAAAGGC 
CCCGAGCCTG ACACCATCAT TGGGTACTCC GGAGAAGATT ATCCCAGGGC TGCGCCCACT 
GAAGTTAAAA TCCGAGTCCT GAACAGCACA GCCATCAGCC TTCAGTGGAA CCGAGTCTAC 
TCTGACACGG TCCAGGGCCA GCTCAGAGAG TATCGAGCTT ACTACTGGAG GGAAAGCAGT 
TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG AGACAGCAGG CCAGCTTCCC TGGTGACCGT 
CCCCGGGGCG TGGTGGCCCG CCTGTTCCCC TACAGTAACT ACAAGCTGGA GATGGTGGTG 
GTCAATGGGA GAGGTGACGG GCCTCGAAGT GAAACCAAGG AATTCACCAC CCCAGAAGGA 
GTACCCAGTG CCCCCAGGCG GTTCAGAGTC CGACAGCCCA ACCTGGAGAC CATCAACCTG 
GAGTGGGACC ACCCAGAGCA CCCCAACGGA ATCCTGATTG GATACATCCT CAGATACGTG 
CCCTTTAATG GAACCAAACT GGGAAAGCAG ATGGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCTCTG TGCAGAGAGC AGACCCAGTG TCGCGTTACC GCTTCTCCCT CAGTGCCAGG 
ACACAGGTGG GCTCTGGAGA AGCAGCCACA GAGGAGTCCC CAGCACCTCC AAATGAAGCT 
ACTCCAACTG CAGCTCCTCC CACGTTGCCC CCGACTACTG TGGGTACCAC AGGCCTTGTG 
AGCAGTACTG ATGCTACTGC CCTTGCTGCC ACCAGTGAAG CCACAACAGT TCCCATCATT 
CCAACCGTCG TACCTACCAC CGTCGCCACC ACCATTGCCA CAACTACTAC AACCACTGCC 
GCCACCACCA CCACCACCAC TACGGAGAGC CCTCCCACTA CCACTGCTGG GACTAAGATT 
CACGAAACCG CCCCCGACGA GCAGTCCATT TGGAACGTCA CAGTGCTCCC CAACAGTAAA 
TGGGCCAACA TCACCTGGAA GCACAATTTC AGGCCTGGAA CTGACTTTGT GGTTGAGTAC 
ATCGACAGCA ACCATACGAA AAAAACTGTC CCTGTTAAGG CCCAGGCCCA GCCTATACAG 
CTGACAGACC TCTTTCCCGG GATGACGTAC ACGTTGCGGG TGTATTCCCG GGACAACGAG 
GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110431.1
CDS	1475..4408
Translation	MVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_030254571.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254571.1

ORF Insert Sequence:

ATGGTGAATG GAGAGCCTTT ACAATCGGCA CCACCCAATC CCAACCGTGA GGTAGCTGGA 
GACACTATCA TCTTCCGGGA TACTCAGATC AGCAGCAGGG CAGTGTACCA ATGTAATACA 
TCCAATGAAC ATGGCTACCT GCTGGCCAAT GCCTTCGTCA GCGTGTTAGA TGTACCCCCT 
CGGATGCTGT CTGCCCGCAA CCAGCTCATC AGGGTGATCC TTTATAACCG GACACGGCTG 
GACTGTCCGT TCTTTGGGTC TCCCATCCCA ACACTCCGAT GGTTTAAGAA TGGGCAAGGA 
AGCAACCTGG ATGGCGGTAA CTACCACGTC TACGAAAACG GCAGTCTAGA AATCAAGATG 
ATTCGCAAAG AGGACCAAGG CATCTACACC TGTGTGGCCA CCAACATCCT GGGCAAAGCC 
GAAAATCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA TCTACAGGAT GCCCGAGGAC 
CAGGTGGCCA AGAGGGGCAC CACGGTGCAG CTGGAGTGCC GCGTGAAACA TGACCCCTCC 
TTGAAGCTCA CAGTCTCCTG GCTGAAGGAC GATGAGCCAC TCTACATTGG AAACAGGATG 
AAGAAGGAAG ATGACTCCCT GACGATCTTC GGAGTGGCAG AGCGGGACCA GGGCAGTTAC 
ACGTGTATGG CCAGCACCGA GCTGGACCAG GACCTGGCAA AGGCCTACCT CACTGTTCTA 
GCTGATCAGG CCACTCCAAC TAACCGTTTG GCTGCCCTAC CCAAAGGGCG ACCAGACCGA 
CCCAGGGACC TGGAGCTCAC TGACCTGGCT GAGAGGAGTG TGAGGCTGAC CTGGATCCCA 
GGGGATGACA ACAACAGCCC TATCACAGAC TACGTCGTTC AGTTTGAAGA GGACCAGTTC 
CAACCAGGGG TGTGGCATGA CCACTCCAGG TTCCCAGGCA GCGTCAACTC AGCCGTCCTC 
CATCTGTCCC CATATGTCAA CTACCAATTC AGAGTCATCG CTGTCAACGA GGTTGGGAGC 
AGCCACCCCA GCCTTCCATC CGAGCGGTAC CGAACCAGTG GGGCACCCCC TGAATCTAAT 
CCCAGTGATG TGAAGGGCGA AGGGACAAGA AAGAACAATA TGGAGATCAC GTGGACGCCT 
ATGAATGCTA CCTCTGCCTT TGGCCCCAAC CTACGCTACA TTGTCAAGTG GCGACGGAGA 
GAGACCCGAG AGACTTGGAA CAATGTCACA GTGTGGGGCT CTCGCTACGT GGTGGGGCAG 
ACGCCTGTCT ACGTTCCCTA TGAGATCCGA GTCCAGGCTG AAAATGACTT TGGGAAAGGC 
CCCGAGCCTG ACACCATCAT TGGGTACTCC GGAGAAGATT ATCCCAGGGC TGCGCCCACT 
GAAGTTAAAA TCCGAGTCCT GAACAGCACA GCCATCAGCC TTCAGTGGAA CCGAGTCTAC 
TCTGACACGG TCCAGGGCCA GCTCAGAGAG TATCGAGCTT ACTACTGGAG GGAAAGCAGT 
TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG AGACAGCAGG CCAGCTTCCC TGGTGACCGT 
CCCCGGGGCG TGGTGGCCCG CCTGTTCCCC TACAGTAACT ACAAGCTGGA GATGGTGGTG 
GTCAATGGGA GAGGTGACGG GCCTCGAAGT GAAACCAAGG AATTCACCAC CCCAGAAGGA 
GTACCCAGTG CCCCCAGGCG GTTCAGAGTC CGACAGCCCA ACCTGGAGAC CATCAACCTG 
GAGTGGGACC ACCCAGAGCA CCCCAACGGA ATCCTGATTG GATACATCCT CAGATACGTG 
CCCTTTAATG GAACCAAACT GGGAAAGCAG ATGGTGGAAA ACTTCTCTCC CAATCAGACC 
AAGTTCTCTG TGCAGAGAGC AGACCCAGTG TCGCGTTACC GCTTCTCCCT CAGTGCCAGG 
ACACAGGTGG GCTCTGGAGA AGCAGCCACA GAGGAGTCCC CAGCACCTCC AAATGAAGCT 
ACTCCAACTG CAGCTCCTCC CACGTTGCCC CCGACTACTG TGGGTACCAC AGGCCTTGTG 
AGCAGTACTG ATGCTACTGC CCTTGCTGCC ACCAGTGAAG CCACAACAGT TCCCATCATT 
CCAACCGTCG TACCTACCAC CGTCGCCACC ACCATTGCCA CAACTACTAC AACCACTGCC 
GCCACCACCA CCACCACCAC TACGGAGAGC CCTCCCACTA CCACTGCTGG GACTAAGATT 
CACGAAACCG CCCCCGACGA GCAGTCCATT TGGAACGTCA CAGTGCTCCC CAACAGTAAA 
TGGGCCAACA TCACCTGGAA GCACAATTTC AGGCCTGGAA CTGACTTTGT GGTTGAGTAC 
ATCGACAGCA ACCATACGAA AAAAACTGTC CCTGTTAAGG CCCAGGCCCA GCCTATACAG 
CTGACAGACC TCTTTCCCGG GATGACGTAC ACGTTGCGGG TGTATTCCCG GGACAACGAG 
GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017320907.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176396.1 Mus musculus neurofascin (Nfasc), transcript variant X34, mRNA.

Price & Availability↑

CloneID	OMu77146
Clone ID Related Accession (Same CDS sequence)	XM_017320907.1
Accession Version	XM_017320907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCACC CGAGGAGTTG CAGAAAGAAC GCCCAGCTTC 
ATGTATCCCC AGGGCACATC GAGCAGTCAG ATGGTTCTCC GTGGCATGGA CCTGCTGCTG 
GAATGCATTG CCTCTGGCGT CCCAACACCA GACATTGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCATCTA ACAAGGCCAA GTTCGAGAAC TTTAATAAGG CTCTGCGCAT CACCAATGTC 
TCTGAAGAGG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCT CCATACTGGC TGGATGAGCC CAAGAACCTG 
ATCCTGGCTC CTGGGGAAGA TGGGAGGCTG GTATGCCGAG CCAATGGGAA CCCGAAGCCG 
ACCGTGCAGT GGATGGTGAA TGGAGAGCCT TTACAATCGG CACCACCCAA TCCCAACCGT 
GAGGTAGCTG GAGACACTAT CATCTTCCGG GATACTCAGA TCAGCAGCAG GGCAGTGTAC 
CAATGTAATA CATCCAATGA ACATGGCTAC CTGCTGGCCA ATGCCTTCGT CAGCGTGTTA 
GATGTACCCC CTCGGATGCT GTCTGCCCGC AACCAGCTCA TCAGGGTGAT CCTTTATAAC 
CGGACACGGC TGGACTGTCC GTTCTTTGGG TCTCCCATCC CAACACTCCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGCGGT AACTACCACG TCTACGAAAA CGGCAGTCTA 
GAAATCAAGA TGATTCGCAA AGAGGACCAA GGCATCTACA CCTGTGTGGC CACCAACATC 
CTGGGCAAAG CCGAAAATCA AGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACAGG 
ATGCCCGAGG ACCAGGTGGC CAAGAGGGGC ACCACGGTGC AGCTGGAGTG CCGCGTGAAA 
CATGACCCCT CCTTGAAGCT CACAGTCTCC TGGCTGAAGG ACGATGAGCC ACTCTACATT 
GGAAACAGGA TGAAGAAGGA AGATGACTCC CTGACGATCT TCGGAGTGGC AGAGCGGGAC 
CAGGGCAGTT ACACGTGTAT GGCCAGCACC GAGCTGGACC AGGACCTGGC AAAGGCCTAC 
CTCACTGTTC TAGCTGATCA GGCCACTCCA ACTAACCGTT TGGCTGCCCT ACCCAAAGGG 
CGACCAGACC GACCCAGGGA CCTGGAGCTC ACTGACCTGG CTGAGAGGAG TGTGAGGCTG 
ACCTGGATCC CAGGGGATGA CAACAACAGC CCTATCACAG ACTACGTCGT TCAGTTTGAA 
GAGGACCAGT TCCAACCAGG GGTGTGGCAT GACCACTCCA GGTTCCCAGG CAGCGTCAAC 
TCAGCCGTCC TCCATCTGTC CCCATATGTC AACTACCAAT TCAGAGTCAT CGCTGTCAAC 
GAGGTTGGGA GCAGCCACCC CAGCCTTCCA TCCGAGCGGT ACCGAACCAG TGGGGCACCC 
CCTGAATCTA ATCCCAGTGA TGTGAAGGGC GAAGGGACAA GAAAGAACAA TATGGAGATC 
ACGTGGACGC CTATGAATGC TACCTCTGCC TTTGGCCCCA ACCTACGCTA CATTGTCAAG 
TGGCGACGGA GAGAGACCCG AGAGACTTGG AACAATGTCA CAGTGTGGGG CTCTCGCTAC 
GTGGTGGGGC AGACGCCTGT CTACGTTCCC TATGAGATCC GAGTCCAGGC TGAAAATGAC 
TTTGGGAAAG GCCCCGAGCC TGACACCATC ATTGGGTACT CCGGAGAAGA TTATCCCAGG 
GCTGCGCCCA CTGAAGTTAA AATCCGAGTC CTGAACAGCA CAGCCATCAG CCTTCAGTGG 
AACCGAGTCT ACTCTGACAC GGTCCAGGGC CAGCTCAGAG AGTATCGAGT GAGAAGGCTA 
AGCACCCAGC CCCACGTAAC CACCTTCTTG CTAACCAGGG AAAGGGGTAG CAGCCGTGGT 
GTGTGTGTGT GTGGGGGGGG GGGTGTCTCC AGGGACCCTC GGAGTACTTG TGGGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176396.1
CDS	212..2911
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRVRRLSTQPHVTTFLLTRERGSSRGVCVCGGGGVSRDPRSTCGI

Target ORF information:

RefSeq Version	XM_017320907.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320907.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCACC CGAGGAGTTG CAGAAAGAAC GCCCAGCTTC 
ATGTATCCCC AGGGCACATC GAGCAGTCAG ATGGTTCTCC GTGGCATGGA CCTGCTGCTG 
GAATGCATTG CCTCTGGCGT CCCAACACCA GACATTGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCATCTA ACAAGGCCAA GTTCGAGAAC TTTAATAAGG CTCTGCGCAT CACCAATGTC 
TCTGAAGAGG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCT CCATACTGGC TGGATGAGCC CAAGAACCTG 
ATCCTGGCTC CTGGGGAAGA TGGGAGGCTG GTATGCCGAG CCAATGGGAA CCCGAAGCCG 
ACCGTGCAGT GGATGGTGAA TGGAGAGCCT TTACAATCGG CACCACCCAA TCCCAACCGT 
GAGGTAGCTG GAGACACTAT CATCTTCCGG GATACTCAGA TCAGCAGCAG GGCAGTGTAC 
CAATGTAATA CATCCAATGA ACATGGCTAC CTGCTGGCCA ATGCCTTCGT CAGCGTGTTA 
GATGTACCCC CTCGGATGCT GTCTGCCCGC AACCAGCTCA TCAGGGTGAT CCTTTATAAC 
CGGACACGGC TGGACTGTCC GTTCTTTGGG TCTCCCATCC CAACACTCCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGCGGT AACTACCACG TCTACGAAAA CGGCAGTCTA 
GAAATCAAGA TGATTCGCAA AGAGGACCAA GGCATCTACA CCTGTGTGGC CACCAACATC 
CTGGGCAAAG CCGAAAATCA AGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACAGG 
ATGCCCGAGG ACCAGGTGGC CAAGAGGGGC ACCACGGTGC AGCTGGAGTG CCGCGTGAAA 
CATGACCCCT CCTTGAAGCT CACAGTCTCC TGGCTGAAGG ACGATGAGCC ACTCTACATT 
GGAAACAGGA TGAAGAAGGA AGATGACTCC CTGACGATCT TCGGAGTGGC AGAGCGGGAC 
CAGGGCAGTT ACACGTGTAT GGCCAGCACC GAGCTGGACC AGGACCTGGC AAAGGCCTAC 
CTCACTGTTC TAGCTGATCA GGCCACTCCA ACTAACCGTT TGGCTGCCCT ACCCAAAGGG 
CGACCAGACC GACCCAGGGA CCTGGAGCTC ACTGACCTGG CTGAGAGGAG TGTGAGGCTG 
ACCTGGATCC CAGGGGATGA CAACAACAGC CCTATCACAG ACTACGTCGT TCAGTTTGAA 
GAGGACCAGT TCCAACCAGG GGTGTGGCAT GACCACTCCA GGTTCCCAGG CAGCGTCAAC 
TCAGCCGTCC TCCATCTGTC CCCATATGTC AACTACCAAT TCAGAGTCAT CGCTGTCAAC 
GAGGTTGGGA GCAGCCACCC CAGCCTTCCA TCCGAGCGGT ACCGAACCAG TGGGGCACCC 
CCTGAATCTA ATCCCAGTGA TGTGAAGGGC GAAGGGACAA GAAAGAACAA TATGGAGATC 
ACGTGGACGC CTATGAATGC TACCTCTGCC TTTGGCCCCA ACCTACGCTA CATTGTCAAG 
TGGCGACGGA GAGAGACCCG AGAGACTTGG AACAATGTCA CAGTGTGGGG CTCTCGCTAC 
GTGGTGGGGC AGACGCCTGT CTACGTTCCC TATGAGATCC GAGTCCAGGC TGAAAATGAC 
TTTGGGAAAG GCCCCGAGCC TGACACCATC ATTGGGTACT CCGGAGAAGA TTATCCCAGG 
GCTGCGCCCA CTGAAGTTAA AATCCGAGTC CTGAACAGCA CAGCCATCAG CCTTCAGTGG 
AACCGAGTCT ACTCTGACAC GGTCCAGGGC CAGCTCAGAG AGTATCGAGT GAGAAGGCTA 
AGCACCCAGC CCCACGTAAC CACCTTCTTG CTAACCAGGG AAAGGGGTAG CAGCCGTGGT 
GTGTGTGTGT GTGGGGGGGG GGGTGTCTCC AGGGACCCTC GGAGTACTTG TGGGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017320909.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176398.1 Mus musculus neurofascin (Nfasc), transcript variant X40, mRNA.

Price & Availability↑

CloneID	OMu77147
Clone ID Related Accession (Same CDS sequence)	XM_017320909.2 , XM_017320909.1
Accession Version	XM_017320909.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCA CCTTCATACC ACCAAGTTGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176398.1
CDS	53..2266
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLAPSYHQVD

Target ORF information:

RefSeq Version	XM_017320909.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320909.2

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCA CCTTCATACC ACCAAGTTGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017320909.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176398.1 Mus musculus neurofascin (Nfasc), transcript variant X35, mRNA.

Price & Availability↑

CloneID	OMu77147
Clone ID Related Accession (Same CDS sequence)	XM_017320909.2 , XM_017320909.1
Accession Version	XM_017320909.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCA CCTTCATACC ACCAAGTTGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176398.1
CDS	56..2269
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLAPSYHQVD

Target ORF information:

RefSeq Version	XM_017320909.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320909.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCA CCTTCATACC ACCAAGTTGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006529634.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529697.2 Mus musculus neurofascin (Nfasc), transcript variant X1, mRNA.

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CloneID	OMu74366
Clone ID Related Accession (Same CDS sequence)	XM_006529634.3 , XM_006529634.2
Accession Version	XM_006529634.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529634.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC GTTGCCCCCG 
ACTACTGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC 
AGTGAAGCCA CAACAGTTCC CATCATTCCA ACCGTCGTAC CTACCACCGT CGCCACCACC 
ATTGCCACAA CTACTACAAC CACTGCCGCC ACCACCACCA CCACCACTAC GGAGAGCCCT 
CCCACTACCA CTGCTGGGAC TAAGATTCAC GAAACCGCCC CCGACGAGCA GTCCATTTGG 
AACGTCACAG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCAGG 
CCTGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AGGCCCAGCC TATACAGCTG ACAGACCTCT TTCCCGGGAT GACGTACACG 
TTGCGGGTGT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC 
AGTACAGCTT ACACCAATAA CCAGGCAGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC 
ATGTGTGCCA TTGCCCTTCT GGTGCTGATC CTTCTCATCG TCTGCTTCAT CAAGAGGAGT 
CGAGGTGGCA AGTACCCAGT GCGGGAAAAG AAGGATGTCC CCTTGGGTCC TGAAGACCCC 
AAAGAAGAAG ATGGCTCATT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACATCTC TGGATGGCAC CATCAAGCAG CAGGAGAGCG ATGACAGCCT GGTGGACTAT 
GGCGAAGGCG GCGAGGGCCA GTTCAATGAA GATGGCTCCT TTATTGGCCA GTACACTGTC 
AAAAAGGACA AGGAGGAAAC GGAGGGCAAT GAGAGCTCAG AGGCCACATC ACCAGTCAAT 
GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529697.2
CDS	53..4453
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006529634.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529634.3

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC GTTGCCCCCG 
ACTACTGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC 
AGTGAAGCCA CAACAGTTCC CATCATTCCA ACCGTCGTAC CTACCACCGT CGCCACCACC 
ATTGCCACAA CTACTACAAC CACTGCCGCC ACCACCACCA CCACCACTAC GGAGAGCCCT 
CCCACTACCA CTGCTGGGAC TAAGATTCAC GAAACCGCCC CCGACGAGCA GTCCATTTGG 
AACGTCACAG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCAGG 
CCTGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AGGCCCAGCC TATACAGCTG ACAGACCTCT TTCCCGGGAT GACGTACACG 
TTGCGGGTGT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC 
AGTACAGCTT ACACCAATAA CCAGGCAGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC 
ATGTGTGCCA TTGCCCTTCT GGTGCTGATC CTTCTCATCG TCTGCTTCAT CAAGAGGAGT 
CGAGGTGGCA AGTACCCAGT GCGGGAAAAG AAGGATGTCC CCTTGGGTCC TGAAGACCCC 
AAAGAAGAAG ATGGCTCATT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACATCTC TGGATGGCAC CATCAAGCAG CAGGAGAGCG ATGACAGCCT GGTGGACTAT 
GGCGAAGGCG GCGAGGGCCA GTTCAATGAA GATGGCTCCT TTATTGGCCA GTACACTGTC 
AAAAAGGACA AGGAGGAAAC GGAGGGCAAT GAGAGCTCAG AGGCCACATC ACCAGTCAAT 
GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248011.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246313.1 Mus musculus neurofascin (Nfasc), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu74367
Clone ID Related Accession (Same CDS sequence)	XM_011248011.1 , XM_011248011.2
Accession Version	XM_011248011.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG CTGATCAGGC CACTCCAACT AACCGTTTGG CTGCCCTACC CAAAGGGCGA 
CCAGACCGAC CCAGGGACCT GGAGCTCACT GACCTGGCTG AGAGGAGTGT GAGGCTGACC 
TGGATCCCAG GGGATGACAA CAACAGCCCT ATCACAGACT ACGTCGTTCA GTTTGAAGAG 
GACCAGTTCC AACCAGGGGT GTGGCATGAC CACTCCAGGT TCCCAGGCAG CGTCAACTCA 
GCCGTCCTCC ATCTGTCCCC ATATGTCAAC TACCAATTCA GAGTCATCGC TGTCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTTCCATCC GAGCGGTACC GAACCAGTGG GGCACCCCCT 
GAATCTAATC CCAGTGATGT GAAGGGCGAA GGGACAAGAA AGAACAATAT GGAGATCACG 
TGGACGCCTA TGAATGCTAC CTCTGCCTTT GGCCCCAACC TACGCTACAT TGTCAAGTGG 
CGACGGAGAG AGACCCGAGA GACTTGGAAC AATGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CGCCTGTCTA CGTTCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTT 
GGGAAAGGCC CCGAGCCTGA CACCATCATT GGGTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAAT CCGAGTCCTG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGAGTCTACT CTGACACGGT CCAGGGCCAG CTCAGAGAGT ATCGAGCTTA CTACTGGAGG 
GAAAGCAGTT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAGGC CAGCTTCCCT 
GGTGACCGTC CCCGGGGCGT GGTGGCCCGC CTGTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTGGTGG TCAATGGGAG AGGTGACGGG CCTCGAAGTG AAACCAAGGA ATTCACCACC 
CCAGAAGGAG TACCCAGTGC CCCCAGGCGG TTCAGAGTCC GACAGCCCAA CCTGGAGACC 
ATCAACCTGG AGTGGGACCA CCCAGAGCAC CCCAACGGAA TCCTGATTGG ATACATCCTC 
AGATACGTGC CCTTTAATGG AACCAAACTG GGAAAGCAGA TGGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCTCTGT GCAGAGAGCA GACCCAGTGT CGCGTTACCG CTTCTCCCTC 
AGTGCCAGGA CACAGGTGGG CTCTGGAGAA GCAGCCACAG AGGAGTCCCC AGCACCTCCA 
AATGAAGCTA CTCCAACTGC AGCTCCTCCC ACGTTGCCCC CGACTACTGT GGGTACCACA 
GGCCTTGTGA GCAGTACTGA TGCTACTGCC CTTGCTGCCA CCAGTGAAGC CACAACAGTT 
CCCATCATTC CAACCGTCGT ACCTACCACC GTCGCCACCA CCATTGCCAC AACTACTACA 
ACCACTGCCG CCACCACCAC CACCACCACT ACGGAGAGCC CTCCCACTAC CACTGCTGGG 
ACTAAGATTC ACGAAACCGC CCCCGACGAG CAGTCCATTT GGAACGTCAC AGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCA GGCCTGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCTATACAGC TGACAGACCT CTTTCCCGGG ATGACGTACA CGTTGCGGGT GTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT 
AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT 
CTGGTGCTGA TCCTTCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAGTACCCA 
GTGCGGGAAA AGAAGGATGT CCCCTTGGGT CCTGAAGACC CCAAAGAAGA AGATGGCTCA 
TTTGACTACA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGATGGC 
ACCATCAAGC AGCAGGAGAG CGATGACAGC CTGGTGGACT ATGGCGAAGG CGGCGAGGGC 
CAGTTCAATG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAAAAAGGA CAAGGAGGAA 
ACGGAGGGCA ATGAGAGCTC AGAGGCCACA TCACCAGTCA ATGCCATCTA TTCCCTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246313.1
CDS	51..4433
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248011.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248011.2

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG CTGATCAGGC CACTCCAACT AACCGTTTGG CTGCCCTACC CAAAGGGCGA 
CCAGACCGAC CCAGGGACCT GGAGCTCACT GACCTGGCTG AGAGGAGTGT GAGGCTGACC 
TGGATCCCAG GGGATGACAA CAACAGCCCT ATCACAGACT ACGTCGTTCA GTTTGAAGAG 
GACCAGTTCC AACCAGGGGT GTGGCATGAC CACTCCAGGT TCCCAGGCAG CGTCAACTCA 
GCCGTCCTCC ATCTGTCCCC ATATGTCAAC TACCAATTCA GAGTCATCGC TGTCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTTCCATCC GAGCGGTACC GAACCAGTGG GGCACCCCCT 
GAATCTAATC CCAGTGATGT GAAGGGCGAA GGGACAAGAA AGAACAATAT GGAGATCACG 
TGGACGCCTA TGAATGCTAC CTCTGCCTTT GGCCCCAACC TACGCTACAT TGTCAAGTGG 
CGACGGAGAG AGACCCGAGA GACTTGGAAC AATGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CGCCTGTCTA CGTTCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTT 
GGGAAAGGCC CCGAGCCTGA CACCATCATT GGGTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAAT CCGAGTCCTG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGAGTCTACT CTGACACGGT CCAGGGCCAG CTCAGAGAGT ATCGAGCTTA CTACTGGAGG 
GAAAGCAGTT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAGGC CAGCTTCCCT 
GGTGACCGTC CCCGGGGCGT GGTGGCCCGC CTGTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTGGTGG TCAATGGGAG AGGTGACGGG CCTCGAAGTG AAACCAAGGA ATTCACCACC 
CCAGAAGGAG TACCCAGTGC CCCCAGGCGG TTCAGAGTCC GACAGCCCAA CCTGGAGACC 
ATCAACCTGG AGTGGGACCA CCCAGAGCAC CCCAACGGAA TCCTGATTGG ATACATCCTC 
AGATACGTGC CCTTTAATGG AACCAAACTG GGAAAGCAGA TGGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCTCTGT GCAGAGAGCA GACCCAGTGT CGCGTTACCG CTTCTCCCTC 
AGTGCCAGGA CACAGGTGGG CTCTGGAGAA GCAGCCACAG AGGAGTCCCC AGCACCTCCA 
AATGAAGCTA CTCCAACTGC AGCTCCTCCC ACGTTGCCCC CGACTACTGT GGGTACCACA 
GGCCTTGTGA GCAGTACTGA TGCTACTGCC CTTGCTGCCA CCAGTGAAGC CACAACAGTT 
CCCATCATTC CAACCGTCGT ACCTACCACC GTCGCCACCA CCATTGCCAC AACTACTACA 
ACCACTGCCG CCACCACCAC CACCACCACT ACGGAGAGCC CTCCCACTAC CACTGCTGGG 
ACTAAGATTC ACGAAACCGC CCCCGACGAG CAGTCCATTT GGAACGTCAC AGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCA GGCCTGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCTATACAGC TGACAGACCT CTTTCCCGGG ATGACGTACA CGTTGCGGGT GTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT 
AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT 
CTGGTGCTGA TCCTTCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAGTACCCA 
GTGCGGGAAA AGAAGGATGT CCCCTTGGGT CCTGAAGACC CCAAAGAAGA AGATGGCTCA 
TTTGACTACA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGATGGC 
ACCATCAAGC AGCAGGAGAG CGATGACAGC CTGGTGGACT ATGGCGAAGG CGGCGAGGGC 
CAGTTCAATG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAAAAAGGA CAAGGAGGAA 
ACGGAGGGCA ATGAGAGCTC AGAGGCCACA TCACCAGTCA ATGCCATCTA TTCCCTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248012.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246314.1 Mus musculus neurofascin (Nfasc), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu74368
Clone ID Related Accession (Same CDS sequence)	XM_011248012.2 , XM_011248012.1
Accession Version	XM_011248012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGGG CGACCAGACC GACCCAGGGA CCTGGAGCTC 
ACTGACCTGG CTGAGAGGAG TGTGAGGCTG ACCTGGATCC CAGGGGATGA CAACAACAGC 
CCTATCACAG ACTACGTCGT TCAGTTTGAA GAGGACCAGT TCCAACCAGG GGTGTGGCAT 
GACCACTCCA GGTTCCCAGG CAGCGTCAAC TCAGCCGTCC TCCATCTGTC CCCATATGTC 
AACTACCAAT TCAGAGTCAT CGCTGTCAAC GAGGTTGGGA GCAGCCACCC CAGCCTTCCA 
TCCGAGCGGT ACCGAACCAG TGGGGCACCC CCTGAATCTA ATCCCAGTGA TGTGAAGGGC 
GAAGGGACAA GAAAGAACAA TATGGAGATC ACGTGGACGC CTATGAATGC TACCTCTGCC 
TTTGGCCCCA ACCTACGCTA CATTGTCAAG TGGCGACGGA GAGAGACCCG AGAGACTTGG 
AACAATGTCA CAGTGTGGGG CTCTCGCTAC GTGGTGGGGC AGACGCCTGT CTACGTTCCC 
TATGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAAG GCCCCGAGCC TGACACCATC 
ATTGGGTACT CCGGAGAAGA TTATCCCAGG GCTGCGCCCA CTGAAGTTAA AATCCGAGTC 
CTGAACAGCA CAGCCATCAG CCTTCAGTGG AACCGAGTCT ACTCTGACAC GGTCCAGGGC 
CAGCTCAGAG AGTATCGAGC TTACTACTGG AGGGAAAGCA GTTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGACAGCA GGCCAGCTTC CCTGGTGACC GTCCCCGGGG CGTGGTGGCC 
CGCCTGTTCC CCTACAGTAA CTACAAGCTG GAGATGGTGG TGGTCAATGG GAGAGGTGAC 
GGGCCTCGAA GTGAAACCAA GGAATTCACC ACCCCAGAAG GAGTACCCAG TGCCCCCAGG 
CGGTTCAGAG TCCGACAGCC CAACCTGGAG ACCATCAACC TGGAGTGGGA CCACCCAGAG 
CACCCCAACG GAATCCTGAT TGGATACATC CTCAGATACG TGCCCTTTAA TGGAACCAAA 
CTGGGAAAGC AGATGGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCTC TGTGCAGAGA 
GCAGACCCAG TGTCGCGTTA CCGCTTCTCC CTCAGTGCCA GGACACAGGT GGGCTCTGGA 
GAAGCAGCCA CAGAGGAGTC CCCAGCACCT CCAAATGAAG CTACTCCAAC TGCAGCTCCT 
CCCACGTTGC CCCCGACTAC TGTGGGTACC ACAGGCCTTG TGAGCAGTAC TGATGCTACT 
GCCCTTGCTG CCACCAGTGA AGCCACAACA GTTCCCATCA TTCCAACCGT CGTACCTACC 
ACCGTCGCCA CCACCATTGC CACAACTACT ACAACCACTG CCGCCACCAC CACCACCACC 
ACTACGGAGA GCCCTCCCAC TACCACTGCT GGGACTAAGA TTCACGAAAC CGCCCCCGAC 
GAGCAGTCCA TTTGGAACGT CACAGTGCTC CCCAACAGTA AATGGGCCAA CATCACCTGG 
AAGCACAATT TCAGGCCTGG AACTGACTTT GTGGTTGAGT ACATCGACAG CAACCATACG 
AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCTATAC AGCTGACAGA CCTCTTTCCC 
GGGATGACGT ACACGTTGCG GGTGTATTCC CGGGACAACG AGGGCATCAG CAGTACCGTC 
ATCACCTTTA TGACCAGTAC AGCTTACACC AATAACCAGG CAGACATCGC CACCCAGGGC 
TGGTTCATCG GGCTCATGTG TGCCATTGCC CTTCTGGTGC TGATCCTTCT CATCGTCTGC 
TTCATCAAGA GGAGTCGAGG TGGCAAGTAC CCAGTGCGGG AAAAGAAGGA TGTCCCCTTG 
GGTCCTGAAG ACCCCAAAGA AGAAGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC ATCTCTGGAT GGCACCATCA AGCAGCAGGA GAGCGATGAC 
AGCCTGGTGG ACTATGGCGA AGGCGGCGAG GGCCAGTTCA ATGAAGATGG CTCCTTTATT 
GGCCAGTACA CTGTCAAAAA GGACAAGGAG GAAACGGAGG GCAATGAGAG CTCAGAGGCC 
ACATCACCAG TCAATGCCAT CTATTCCCTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246314.1
CDS	53..4408
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248012.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248012.2

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGGG CGACCAGACC GACCCAGGGA CCTGGAGCTC 
ACTGACCTGG CTGAGAGGAG TGTGAGGCTG ACCTGGATCC CAGGGGATGA CAACAACAGC 
CCTATCACAG ACTACGTCGT TCAGTTTGAA GAGGACCAGT TCCAACCAGG GGTGTGGCAT 
GACCACTCCA GGTTCCCAGG CAGCGTCAAC TCAGCCGTCC TCCATCTGTC CCCATATGTC 
AACTACCAAT TCAGAGTCAT CGCTGTCAAC GAGGTTGGGA GCAGCCACCC CAGCCTTCCA 
TCCGAGCGGT ACCGAACCAG TGGGGCACCC CCTGAATCTA ATCCCAGTGA TGTGAAGGGC 
GAAGGGACAA GAAAGAACAA TATGGAGATC ACGTGGACGC CTATGAATGC TACCTCTGCC 
TTTGGCCCCA ACCTACGCTA CATTGTCAAG TGGCGACGGA GAGAGACCCG AGAGACTTGG 
AACAATGTCA CAGTGTGGGG CTCTCGCTAC GTGGTGGGGC AGACGCCTGT CTACGTTCCC 
TATGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAAG GCCCCGAGCC TGACACCATC 
ATTGGGTACT CCGGAGAAGA TTATCCCAGG GCTGCGCCCA CTGAAGTTAA AATCCGAGTC 
CTGAACAGCA CAGCCATCAG CCTTCAGTGG AACCGAGTCT ACTCTGACAC GGTCCAGGGC 
CAGCTCAGAG AGTATCGAGC TTACTACTGG AGGGAAAGCA GTTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGACAGCA GGCCAGCTTC CCTGGTGACC GTCCCCGGGG CGTGGTGGCC 
CGCCTGTTCC CCTACAGTAA CTACAAGCTG GAGATGGTGG TGGTCAATGG GAGAGGTGAC 
GGGCCTCGAA GTGAAACCAA GGAATTCACC ACCCCAGAAG GAGTACCCAG TGCCCCCAGG 
CGGTTCAGAG TCCGACAGCC CAACCTGGAG ACCATCAACC TGGAGTGGGA CCACCCAGAG 
CACCCCAACG GAATCCTGAT TGGATACATC CTCAGATACG TGCCCTTTAA TGGAACCAAA 
CTGGGAAAGC AGATGGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCTC TGTGCAGAGA 
GCAGACCCAG TGTCGCGTTA CCGCTTCTCC CTCAGTGCCA GGACACAGGT GGGCTCTGGA 
GAAGCAGCCA CAGAGGAGTC CCCAGCACCT CCAAATGAAG CTACTCCAAC TGCAGCTCCT 
CCCACGTTGC CCCCGACTAC TGTGGGTACC ACAGGCCTTG TGAGCAGTAC TGATGCTACT 
GCCCTTGCTG CCACCAGTGA AGCCACAACA GTTCCCATCA TTCCAACCGT CGTACCTACC 
ACCGTCGCCA CCACCATTGC CACAACTACT ACAACCACTG CCGCCACCAC CACCACCACC 
ACTACGGAGA GCCCTCCCAC TACCACTGCT GGGACTAAGA TTCACGAAAC CGCCCCCGAC 
GAGCAGTCCA TTTGGAACGT CACAGTGCTC CCCAACAGTA AATGGGCCAA CATCACCTGG 
AAGCACAATT TCAGGCCTGG AACTGACTTT GTGGTTGAGT ACATCGACAG CAACCATACG 
AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCTATAC AGCTGACAGA CCTCTTTCCC 
GGGATGACGT ACACGTTGCG GGTGTATTCC CGGGACAACG AGGGCATCAG CAGTACCGTC 
ATCACCTTTA TGACCAGTAC AGCTTACACC AATAACCAGG CAGACATCGC CACCCAGGGC 
TGGTTCATCG GGCTCATGTG TGCCATTGCC CTTCTGGTGC TGATCCTTCT CATCGTCTGC 
TTCATCAAGA GGAGTCGAGG TGGCAAGTAC CCAGTGCGGG AAAAGAAGGA TGTCCCCTTG 
GGTCCTGAAG ACCCCAAAGA AGAAGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC ATCTCTGGAT GGCACCATCA AGCAGCAGGA GAGCGATGAC 
AGCCTGGTGG ACTATGGCGA AGGCGGCGAG GGCCAGTTCA ATGAAGATGG CTCCTTTATT 
GGCCAGTACA CTGTCAAAAA GGACAAGGAG GAAACGGAGG GCAATGAGAG CTCAGAGGCC 
ACATCACCAG TCAATGCCAT CTATTCCCTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006529635.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529698.2 Mus musculus neurofascin (Nfasc), transcript variant X4, mRNA.

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CloneID	OMu74369
Clone ID Related Accession (Same CDS sequence)	XM_006529635.4 , XM_006529635.3
Accession Version	XM_006529635.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529635.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CACCCGAGGA 
GTTGCAGAAA GAACGCCCAG CTTCATGTAT CCCCAGGGCA CATCGAGCAG TCAGATGGTT 
CTCCGTGGCA TGGACCTGCT GCTGGAATGC ATTGCCTCTG GCGTCCCAAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTAACAAGG CCAAGTTCGA GAACTTTAAT 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCTCCATAC 
TGGCTGGATG AGCCCAAGAA CCTGATCCTG GCTCCTGGGG AAGATGGGAG GCTGGTATGC 
CGAGCCAATG GGAACCCGAA GCCGACCGTG CAGTGGATGG TGAATGGAGA GCCTTTACAA 
TCGGCACCAC CCAATCCCAA CCGTGAGGTA GCTGGAGACA CTATCATCTT CCGGGATACT 
CAGATCAGCA GCAGGGCAGT GTACCAATGT AATACATCCA ATGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT GTTAGATGTA CCCCCTCGGA TGCTGTCTGC CCGCAACCAG 
CTCATCAGGG TGATCCTTTA TAACCGGACA CGGCTGGACT GTCCGTTCTT TGGGTCTCCC 
ATCCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG CGGTAACTAC 
CACGTCTACG AAAACGGCAG TCTAGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGC AAAGCCGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCC GAGGACCAGG TGGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAACATGAC CCCTCCTTGA AGCTCACAGT CTCCTGGCTG 
AAGGACGATG AGCCACTCTA CATTGGAAAC AGGATGAAGA AGGAAGATGA CTCCCTGACG 
ATCTTCGGAG TGGCAGAGCG GGACCAGGGC AGTTACACGT GTATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTACCCAA AGGGCGACCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCTGAGA GGAGTGTGAG GCTGACCTGG ATCCCAGGGG ATGACAACAA CAGCCCTATC 
ACAGACTACG TCGTTCAGTT TGAAGAGGAC CAGTTCCAAC CAGGGGTGTG GCATGACCAC 
TCCAGGTTCC CAGGCAGCGT CAACTCAGCC GTCCTCCATC TGTCCCCATA TGTCAACTAC 
CAATTCAGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CCAGTGGGGC ACCCCCTGAA TCTAATCCCA GTGATGTGAA GGGCGAAGGG 
ACAAGAAAGA ACAATATGGA GATCACGTGG ACGCCTATGA ATGCTACCTC TGCCTTTGGC 
CCCAACCTAC GCTACATTGT CAAGTGGCGA CGGAGAGAGA CCCGAGAGAC TTGGAACAAT 
GTCACAGTGT GGGGCTCTCG CTACGTGGTG GGGCAGACGC CTGTCTACGT TCCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCCG AGCCTGACAC CATCATTGGG 
TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGA GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGT GACCGTCCCC GGGGCGTGGT GGCCCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTGGTGGTCA ATGGGAGAGG TGACGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCA GAAGGAGTAC CCAGTGCCCC CAGGCGGTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAGT GGGACCACCC AGAGCACCCC 
AACGGAATCC TGATTGGATA CATCCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCTGTGCA GAGAGCAGAC 
CCAGTGTCGC GTTACCGCTT CTCCCTCAGT GCCAGGACAC AGGTGGGCTC TGGAGAAGCA 
GCCACAGAGG AGTCCCCAGC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACG 
TTGCCCCCGA CTACTGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT 
GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATTCCAA CCGTCGTACC TACCACCGTC 
GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCA CCACCACCAC CACCACTACG 
GAGAGCCCTC CCACTACCAC TGCTGGGACT AAGATTCACG AAACCGCCCC CGACGAGCAG 
TCCATTTGGA ACGTCACAGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC 
AATTTCAGGC CTGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA 
ACTGTCCCTG TTAAGGCCCA GGCCCAGCCT ATACAGCTGA CAGACCTCTT TCCCGGGATG 
ACGTACACGT TGCGGGTGTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC 
TTTATGACCA GTACAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TTCTCATCGT CTGCTTCATC 
AAGAGGAGTC GAGGTGGCAA GTACCCAGTG CGGGAAAAGA AGGATGTCCC CTTGGGTCCT 
GAAGACCCCA AAGAAGAAGA TGGCTCATTT GACTACAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGATGGCACC ATCAAGCAGC AGGAGAGCGA TGACAGCCTG 
GTGGACTATG GCGAAGGCGG CGAGGGCCAG TTCAATGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA AAAAGGACAA GGAGGAAACG GAGGGCAATG AGAGCTCAGA GGCCACATCA 
CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529698.2
CDS	53..4402
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006529635.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529635.4

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CACCCGAGGA 
GTTGCAGAAA GAACGCCCAG CTTCATGTAT CCCCAGGGCA CATCGAGCAG TCAGATGGTT 
CTCCGTGGCA TGGACCTGCT GCTGGAATGC ATTGCCTCTG GCGTCCCAAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTAACAAGG CCAAGTTCGA GAACTTTAAT 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCTCCATAC 
TGGCTGGATG AGCCCAAGAA CCTGATCCTG GCTCCTGGGG AAGATGGGAG GCTGGTATGC 
CGAGCCAATG GGAACCCGAA GCCGACCGTG CAGTGGATGG TGAATGGAGA GCCTTTACAA 
TCGGCACCAC CCAATCCCAA CCGTGAGGTA GCTGGAGACA CTATCATCTT CCGGGATACT 
CAGATCAGCA GCAGGGCAGT GTACCAATGT AATACATCCA ATGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT GTTAGATGTA CCCCCTCGGA TGCTGTCTGC CCGCAACCAG 
CTCATCAGGG TGATCCTTTA TAACCGGACA CGGCTGGACT GTCCGTTCTT TGGGTCTCCC 
ATCCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG CGGTAACTAC 
CACGTCTACG AAAACGGCAG TCTAGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGC AAAGCCGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCC GAGGACCAGG TGGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAACATGAC CCCTCCTTGA AGCTCACAGT CTCCTGGCTG 
AAGGACGATG AGCCACTCTA CATTGGAAAC AGGATGAAGA AGGAAGATGA CTCCCTGACG 
ATCTTCGGAG TGGCAGAGCG GGACCAGGGC AGTTACACGT GTATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTACCCAA AGGGCGACCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCTGAGA GGAGTGTGAG GCTGACCTGG ATCCCAGGGG ATGACAACAA CAGCCCTATC 
ACAGACTACG TCGTTCAGTT TGAAGAGGAC CAGTTCCAAC CAGGGGTGTG GCATGACCAC 
TCCAGGTTCC CAGGCAGCGT CAACTCAGCC GTCCTCCATC TGTCCCCATA TGTCAACTAC 
CAATTCAGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CCAGTGGGGC ACCCCCTGAA TCTAATCCCA GTGATGTGAA GGGCGAAGGG 
ACAAGAAAGA ACAATATGGA GATCACGTGG ACGCCTATGA ATGCTACCTC TGCCTTTGGC 
CCCAACCTAC GCTACATTGT CAAGTGGCGA CGGAGAGAGA CCCGAGAGAC TTGGAACAAT 
GTCACAGTGT GGGGCTCTCG CTACGTGGTG GGGCAGACGC CTGTCTACGT TCCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCCG AGCCTGACAC CATCATTGGG 
TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGA GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGT GACCGTCCCC GGGGCGTGGT GGCCCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTGGTGGTCA ATGGGAGAGG TGACGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCA GAAGGAGTAC CCAGTGCCCC CAGGCGGTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAGT GGGACCACCC AGAGCACCCC 
AACGGAATCC TGATTGGATA CATCCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCTGTGCA GAGAGCAGAC 
CCAGTGTCGC GTTACCGCTT CTCCCTCAGT GCCAGGACAC AGGTGGGCTC TGGAGAAGCA 
GCCACAGAGG AGTCCCCAGC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACG 
TTGCCCCCGA CTACTGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT 
GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATTCCAA CCGTCGTACC TACCACCGTC 
GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCA CCACCACCAC CACCACTACG 
GAGAGCCCTC CCACTACCAC TGCTGGGACT AAGATTCACG AAACCGCCCC CGACGAGCAG 
TCCATTTGGA ACGTCACAGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC 
AATTTCAGGC CTGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA 
ACTGTCCCTG TTAAGGCCCA GGCCCAGCCT ATACAGCTGA CAGACCTCTT TCCCGGGATG 
ACGTACACGT TGCGGGTGTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC 
TTTATGACCA GTACAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TTCTCATCGT CTGCTTCATC 
AAGAGGAGTC GAGGTGGCAA GTACCCAGTG CGGGAAAAGA AGGATGTCCC CTTGGGTCCT 
GAAGACCCCA AAGAAGAAGA TGGCTCATTT GACTACAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGATGGCACC ATCAAGCAGC AGGAGAGCGA TGACAGCCTG 
GTGGACTATG GCGAAGGCGG CGAGGGCCAG TTCAATGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA AAAAGGACAA GGAGGAAACG GAGGGCAATG AGAGCTCAGA GGCCACATCA 
CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248013.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246315.1 Mus musculus neurofascin (Nfasc), transcript variant X6, mRNA.

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CloneID	OMu74370
Clone ID Related Accession (Same CDS sequence)	XM_011248013.3 , XM_011248013.2
Accession Version	XM_011248013.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248013.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCAGA AAGAACGCCC 
AGCTTCATGT ATCCCCAGGG CACATCGAGC AGTCAGATGG TTCTCCGTGG CATGGACCTG 
CTGCTGGAAT GCATTGCCTC TGGCGTCCCA ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTAACAA GGCCAAGTTC GAGAACTTTA ATAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCAT ACTGGCTGGA TGAGCCCAAG 
AACCTGATCC TGGCTCCTGG GGAAGATGGG AGGCTGGTAT GCCGAGCCAA TGGGAACCCG 
AAGCCGACCG TGCAGTGGAT GGTGAATGGA GAGCCTTTAC AATCGGCACC ACCCAATCCC 
AACCGTGAGG TAGCTGGAGA CACTATCATC TTCCGGGATA CTCAGATCAG CAGCAGGGCA 
GTGTACCAAT GTAATACATC CAATGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTGTTAGATG TACCCCCTCG GATGCTGTCT GCCCGCAACC AGCTCATCAG GGTGATCCTT 
TATAACCGGA CACGGCTGGA CTGTCCGTTC TTTGGGTCTC CCATCCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGCGGTAACT ACCACGTCTA CGAAAACGGC 
AGTCTAGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GCAAAGCCGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CCGAGGACCA GGTGGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAACATG ACCCCTCCTT GAAGCTCACA GTCTCCTGGC TGAAGGACGA TGAGCCACTC 
TACATTGGAA ACAGGATGAA GAAGGAAGAT GACTCCCTGA CGATCTTCGG AGTGGCAGAG 
CGGGACCAGG GCAGTTACAC GTGTATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTACCC 
AAAGGGCGAC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG 
AGGCTGACCT GGATCCCAGG GGATGACAAC AACAGCCCTA TCACAGACTA CGTCGTTCAG 
TTTGAAGAGG ACCAGTTCCA ACCAGGGGTG TGGCATGACC ACTCCAGGTT CCCAGGCAGC 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCA TATGTCAACT ACCAATTCAG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACCAGTGGG 
GCACCCCCTG AATCTAATCC CAGTGATGTG AAGGGCGAAG GGACAAGAAA GAACAATATG 
GAGATCACGT GGACGCCTAT GAATGCTACC TCTGCCTTTG GCCCCAACCT ACGCTACATT 
GTCAAGTGGC GACGGAGAGA GACCCGAGAG ACTTGGAACA ATGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC GCCTGTCTAC GTTCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC CGAGCCTGAC ACCATCATTG GGTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GAGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GTGACCGTCC CCGGGGCGTG GTGGCCCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTGGTGGT CAATGGGAGA GGTGACGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGGT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA GTGGGACCAC CCAGAGCACC CCAACGGAAT CCTGATTGGA 
TACATCCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCTGTG CAGAGAGCAG ACCCAGTGTC GCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC ACAGGTGGGC TCTGGAGAAG CAGCCACAGA GGAGTCCCCA 
GCACCTCCAA ATGAAGCTAC TCCAACTGCA GCTCCTCCCA CGTTGCCCCC GACTACTGTG 
GGTACCACAG GCCTTGTGAG CAGTACTGAT GCTACTGCCC TTGCTGCCAC CAGTGAAGCC 
ACAACAGTTC CCATCATTCC AACCGTCGTA CCTACCACCG TCGCCACCAC CATTGCCACA 
ACTACTACAA CCACTGCCGC CACCACCACC ACCACCACTA CGGAGAGCCC TCCCACTACC 
ACTGCTGGGA CTAAGATTCA CGAAACCGCC CCCGACGAGC AGTCCATTTG GAACGTCACA 
GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCAG GCCTGGAACT 
GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC TGTTAAGGCC 
CAGGCCCAGC CTATACAGCT GACAGACCTC TTTCCCGGGA TGACGTACAC GTTGCGGGTG 
TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT 
TACACCAATA ACCAGGCAGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGTGCC 
ATTGCCCTTC TGGTGCTGAT CCTTCTCATC GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC 
AAGTACCCAG TGCGGGAAAA GAAGGATGTC CCCTTGGGTC CTGAAGACCC CAAAGAAGAA 
GATGGCTCAT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCT 
CTGGATGGCA CCATCAAGCA GCAGGAGAGC GATGACAGCC TGGTGGACTA TGGCGAAGGC 
GGCGAGGGCC AGTTCAATGA AGATGGCTCC TTTATTGGCC AGTACACTGT CAAAAAGGAC 
AAGGAGGAAA CGGAGGGCAA TGAGAGCTCA GAGGCCACAT CACCAGTCAA TGCCATCTAT 
TCCCTTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246315.1
CDS	51..4382
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248013.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248013.3

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCAGA AAGAACGCCC 
AGCTTCATGT ATCCCCAGGG CACATCGAGC AGTCAGATGG TTCTCCGTGG CATGGACCTG 
CTGCTGGAAT GCATTGCCTC TGGCGTCCCA ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTAACAA GGCCAAGTTC GAGAACTTTA ATAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCAT ACTGGCTGGA TGAGCCCAAG 
AACCTGATCC TGGCTCCTGG GGAAGATGGG AGGCTGGTAT GCCGAGCCAA TGGGAACCCG 
AAGCCGACCG TGCAGTGGAT GGTGAATGGA GAGCCTTTAC AATCGGCACC ACCCAATCCC 
AACCGTGAGG TAGCTGGAGA CACTATCATC TTCCGGGATA CTCAGATCAG CAGCAGGGCA 
GTGTACCAAT GTAATACATC CAATGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTGTTAGATG TACCCCCTCG GATGCTGTCT GCCCGCAACC AGCTCATCAG GGTGATCCTT 
TATAACCGGA CACGGCTGGA CTGTCCGTTC TTTGGGTCTC CCATCCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGCGGTAACT ACCACGTCTA CGAAAACGGC 
AGTCTAGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GCAAAGCCGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CCGAGGACCA GGTGGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAACATG ACCCCTCCTT GAAGCTCACA GTCTCCTGGC TGAAGGACGA TGAGCCACTC 
TACATTGGAA ACAGGATGAA GAAGGAAGAT GACTCCCTGA CGATCTTCGG AGTGGCAGAG 
CGGGACCAGG GCAGTTACAC GTGTATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTACCC 
AAAGGGCGAC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG 
AGGCTGACCT GGATCCCAGG GGATGACAAC AACAGCCCTA TCACAGACTA CGTCGTTCAG 
TTTGAAGAGG ACCAGTTCCA ACCAGGGGTG TGGCATGACC ACTCCAGGTT CCCAGGCAGC 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCA TATGTCAACT ACCAATTCAG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACCAGTGGG 
GCACCCCCTG AATCTAATCC CAGTGATGTG AAGGGCGAAG GGACAAGAAA GAACAATATG 
GAGATCACGT GGACGCCTAT GAATGCTACC TCTGCCTTTG GCCCCAACCT ACGCTACATT 
GTCAAGTGGC GACGGAGAGA GACCCGAGAG ACTTGGAACA ATGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC GCCTGTCTAC GTTCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC CGAGCCTGAC ACCATCATTG GGTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GAGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GTGACCGTCC CCGGGGCGTG GTGGCCCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTGGTGGT CAATGGGAGA GGTGACGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGGT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA GTGGGACCAC CCAGAGCACC CCAACGGAAT CCTGATTGGA 
TACATCCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCTGTG CAGAGAGCAG ACCCAGTGTC GCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC ACAGGTGGGC TCTGGAGAAG CAGCCACAGA GGAGTCCCCA 
GCACCTCCAA ATGAAGCTAC TCCAACTGCA GCTCCTCCCA CGTTGCCCCC GACTACTGTG 
GGTACCACAG GCCTTGTGAG CAGTACTGAT GCTACTGCCC TTGCTGCCAC CAGTGAAGCC 
ACAACAGTTC CCATCATTCC AACCGTCGTA CCTACCACCG TCGCCACCAC CATTGCCACA 
ACTACTACAA CCACTGCCGC CACCACCACC ACCACCACTA CGGAGAGCCC TCCCACTACC 
ACTGCTGGGA CTAAGATTCA CGAAACCGCC CCCGACGAGC AGTCCATTTG GAACGTCACA 
GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCAG GCCTGGAACT 
GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC TGTTAAGGCC 
CAGGCCCAGC CTATACAGCT GACAGACCTC TTTCCCGGGA TGACGTACAC GTTGCGGGTG 
TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT 
TACACCAATA ACCAGGCAGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGTGCC 
ATTGCCCTTC TGGTGCTGAT CCTTCTCATC GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC 
AAGTACCCAG TGCGGGAAAA GAAGGATGTC CCCTTGGGTC CTGAAGACCC CAAAGAAGAA 
GATGGCTCAT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCT 
CTGGATGGCA CCATCAAGCA GCAGGAGAGC GATGACAGCC TGGTGGACTA TGGCGAAGGC 
GGCGAGGGCC AGTTCAATGA AGATGGCTCC TTTATTGGCC AGTACACTGT CAAAAAGGAC 
AAGGAGGAAA CGGAGGGCAA TGAGAGCTCA GAGGCCACAT CACCAGTCAA TGCCATCTAT 
TCCCTTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006529639.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529702.2 Mus musculus neurofascin (Nfasc), transcript variant X9, mRNA.

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CloneID	OMu74371
Clone ID Related Accession (Same CDS sequence)	XM_006529639.3 , XM_006529639.4
Accession Version	XM_006529639.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529639.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CCCCCGACGA GCAGTCCATT 
TGGAACGTCA CAGTGCTCCC CAACAGTAAA TGGGCCAACA TCACCTGGAA GCACAATTTC 
AGGCCTGGAA CTGACTTTGT GGTTGAGTAC ATCGACAGCA ACCATACGAA AAAAACTGTC 
CCTGTTAAGG CCCAGGCCCA GCCTATACAG CTGACAGACC TCTTTCCCGG GATGACGTAC 
ACGTTGCGGG TGTATTCCCG GGACAACGAG GGCATCAGCA GTACCGTCAT CACCTTTATG 
ACCAGTACAG CTTACACCAA TAACCAGGCA GACATCGCCA CCCAGGGCTG GTTCATCGGG 
CTCATGTGTG CCATTGCCCT TCTGGTGCTG ATCCTTCTCA TCGTCTGCTT CATCAAGAGG 
AGTCGAGGTG GCAAGTACCC AGTGCGGGAA AAGAAGGATG TCCCCTTGGG TCCTGAAGAC 
CCCAAAGAAG AAGATGGCTC ATTTGACTAC AGTGATGAGG ACAACAAGCC CCTGCAGGGC 
AGCCAGACAT CTCTGGATGG CACCATCAAG CAGCAGGAGA GCGATGACAG CCTGGTGGAC 
TATGGCGAAG GCGGCGAGGG CCAGTTCAAT GAAGATGGCT CCTTTATTGG CCAGTACACT 
GTCAAAAAGG ACAAGGAGGA AACGGAGGGC AATGAGAGCT CAGAGGCCAC ATCACCAGTC 
AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529702.2
CDS	53..4216
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006529639.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529639.4

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CCCCCGACGA GCAGTCCATT 
TGGAACGTCA CAGTGCTCCC CAACAGTAAA TGGGCCAACA TCACCTGGAA GCACAATTTC 
AGGCCTGGAA CTGACTTTGT GGTTGAGTAC ATCGACAGCA ACCATACGAA AAAAACTGTC 
CCTGTTAAGG CCCAGGCCCA GCCTATACAG CTGACAGACC TCTTTCCCGG GATGACGTAC 
ACGTTGCGGG TGTATTCCCG GGACAACGAG GGCATCAGCA GTACCGTCAT CACCTTTATG 
ACCAGTACAG CTTACACCAA TAACCAGGCA GACATCGCCA CCCAGGGCTG GTTCATCGGG 
CTCATGTGTG CCATTGCCCT TCTGGTGCTG ATCCTTCTCA TCGTCTGCTT CATCAAGAGG 
AGTCGAGGTG GCAAGTACCC AGTGCGGGAA AAGAAGGATG TCCCCTTGGG TCCTGAAGAC 
CCCAAAGAAG AAGATGGCTC ATTTGACTAC AGTGATGAGG ACAACAAGCC CCTGCAGGGC 
AGCCAGACAT CTCTGGATGG CACCATCAAG CAGCAGGAGA GCGATGACAG CCTGGTGGAC 
TATGGCGAAG GCGGCGAGGG CCAGTTCAAT GAAGATGGCT CCTTTATTGG CCAGTACACT 
GTCAAAAAGG ACAAGGAGGA AACGGAGGGC AATGAGAGCT CAGAGGCCAC ATCACCAGTC 
AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248014.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246316.1 Mus musculus neurofascin (Nfasc), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu74372
Clone ID Related Accession (Same CDS sequence)	XM_011248014.1 , XM_011248014.2
Accession Version	XM_011248014.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC GTTGCCCCCG 
ACTACTGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC 
AGTGAAGCCA CAACAGTTCC CATCATTCCA ACCGTCGTAC CTACCACCGT CGCCACCACC 
ATTGCCACAA CTACTACAAC CACTGCCGCC ACCACCACCA CCACCACTAC GGAGAGCCCT 
CCCACTACCA CTGCTGGGAC TAAGATTCAC GAAACCGCTT ACACCAATAA CCAGGCAGAC 
ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGTGCCA TTGCCCTTCT GGTGCTGATC 
CTTCTCATCG TCTGCTTCAT CAAGAGGAGT CGAGGTGGCA AGTACCCAGT GCGGGAAAAG 
AAGGATGTCC CCTTGGGTCC TGAAGACCCC AAAGAAGAAG ATGGCTCATT TGACTACAGT 
GATGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCTC TGGATGGCAC CATCAAGCAG 
CAGGAGAGCG ATGACAGCCT GGTGGACTAT GGCGAAGGCG GCGAGGGCCA GTTCAATGAA 
GATGGCTCCT TTATTGGCCA GTACACTGTC AAAAAGGACA AGGAGGAAAC GGAGGGCAAT 
GAGAGCTCAG AGGCCACATC ACCAGTCAAT GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246316.1
CDS	53..4183
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248014.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248014.2

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC GTTGCCCCCG 
ACTACTGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC 
AGTGAAGCCA CAACAGTTCC CATCATTCCA ACCGTCGTAC CTACCACCGT CGCCACCACC 
ATTGCCACAA CTACTACAAC CACTGCCGCC ACCACCACCA CCACCACTAC GGAGAGCCCT 
CCCACTACCA CTGCTGGGAC TAAGATTCAC GAAACCGCTT ACACCAATAA CCAGGCAGAC 
ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGTGCCA TTGCCCTTCT GGTGCTGATC 
CTTCTCATCG TCTGCTTCAT CAAGAGGAGT CGAGGTGGCA AGTACCCAGT GCGGGAAAAG 
AAGGATGTCC CCTTGGGTCC TGAAGACCCC AAAGAAGAAG ATGGCTCATT TGACTACAGT 
GATGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCTC TGGATGGCAC CATCAAGCAG 
CAGGAGAGCG ATGACAGCCT GGTGGACTAT GGCGAAGGCG GCGAGGGCCA GTTCAATGAA 
GATGGCTCCT TTATTGGCCA GTACACTGTC AAAAAGGACA AGGAGGAAAC GGAGGGCAAT 
GAGAGCTCAG AGGCCACATC ACCAGTCAAT GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248015.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246317.1 Mus musculus neurofascin (Nfasc), transcript variant X11, mRNA.

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CloneID	OMu74373
Clone ID Related Accession (Same CDS sequence)	XM_011248015.2 , XM_011248015.1
Accession Version	XM_011248015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTTAC CCAGTGCCCC CAGGCGGTTC AGAGTCCGAC AGCCCAACCT GGAGACCATC 
AACCTGGAGT GGGACCACCC AGAGCACCCC AACGGAATCC TGATTGGATA CATCCTCAGA 
TACGTGCCCT TTAATGGAAC CAAACTGGGA AAGCAGATGG TGGAAAACTT CTCTCCCAAT 
CAGACCAAGT TCTCTGTGCA GAGAGCAGAC CCAGTGTCGC GTTACCGCTT CTCCCTCAGT 
GCCAGGACAC AGGTGGGCTC TGGAGAAGCA GCCACAGAGG AGTCCCCAGC ACCTCCAAAT 
GAAGCTACTC CAACTGCAGC TCCTCCCACG TTGCCCCCGA CTACTGTGGG TACCACAGGC 
CTTGTGAGCA GTACTGATGC TACTGCCCTT GCTGCCACCA GTGAAGCCAC AACAGTTCCC 
ATCATTCCAA CCGTCGTACC TACCACCGTC GCCACCACCA TTGCCACAAC TACTACAACC 
ACTGCCGCCA CCACCACCAC CACCACTACG GAGAGCCCTC CCACTACCAC TGCTGGGACT 
AAGATTCACG AAACCGCCCC CGACGAGCAG TCCATTTGGA ACGTCACAGT GCTCCCCAAC 
AGTAAATGGG CCAACATCAC CTGGAAGCAC AATTTCAGGC CTGGAACTGA CTTTGTGGTT 
GAGTACATCG ACAGCAACCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCT 
ATACAGCTGA CAGACCTCTT TCCCGGGATG ACGTACACGT TGCGGGTGTA TTCCCGGGAC 
AACGAGGGCA TCAGCAGTAC CGTCATCACC TTTATGACCA GTACAGCTTA CACCAATAAC 
CAGGCAGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG 
GTGCTGATCC TTCTCATCGT CTGCTTCATC AAGAGGAGTC GAGGTGGCAA GTACCCAGTG 
CGGGAAAAGA AGGATGTCCC CTTGGGTCCT GAAGACCCCA AAGAAGAAGA TGGCTCATTT 
GACTACAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGATGGCACC 
ATCAAGCAGC AGGAGAGCGA TGACAGCCTG GTGGACTATG GCGAAGGCGG CGAGGGCCAG 
TTCAATGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA AAAAGGACAA GGAGGAAACG 
GAGGGCAATG AGAGCTCAGA GGCCACATCA CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246317.1
CDS	53..4132
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248015.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248015.2

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTTAC CCAGTGCCCC CAGGCGGTTC AGAGTCCGAC AGCCCAACCT GGAGACCATC 
AACCTGGAGT GGGACCACCC AGAGCACCCC AACGGAATCC TGATTGGATA CATCCTCAGA 
TACGTGCCCT TTAATGGAAC CAAACTGGGA AAGCAGATGG TGGAAAACTT CTCTCCCAAT 
CAGACCAAGT TCTCTGTGCA GAGAGCAGAC CCAGTGTCGC GTTACCGCTT CTCCCTCAGT 
GCCAGGACAC AGGTGGGCTC TGGAGAAGCA GCCACAGAGG AGTCCCCAGC ACCTCCAAAT 
GAAGCTACTC CAACTGCAGC TCCTCCCACG TTGCCCCCGA CTACTGTGGG TACCACAGGC 
CTTGTGAGCA GTACTGATGC TACTGCCCTT GCTGCCACCA GTGAAGCCAC AACAGTTCCC 
ATCATTCCAA CCGTCGTACC TACCACCGTC GCCACCACCA TTGCCACAAC TACTACAACC 
ACTGCCGCCA CCACCACCAC CACCACTACG GAGAGCCCTC CCACTACCAC TGCTGGGACT 
AAGATTCACG AAACCGCCCC CGACGAGCAG TCCATTTGGA ACGTCACAGT GCTCCCCAAC 
AGTAAATGGG CCAACATCAC CTGGAAGCAC AATTTCAGGC CTGGAACTGA CTTTGTGGTT 
GAGTACATCG ACAGCAACCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCT 
ATACAGCTGA CAGACCTCTT TCCCGGGATG ACGTACACGT TGCGGGTGTA TTCCCGGGAC 
AACGAGGGCA TCAGCAGTAC CGTCATCACC TTTATGACCA GTACAGCTTA CACCAATAAC 
CAGGCAGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG 
GTGCTGATCC TTCTCATCGT CTGCTTCATC AAGAGGAGTC GAGGTGGCAA GTACCCAGTG 
CGGGAAAAGA AGGATGTCCC CTTGGGTCCT GAAGACCCCA AAGAAGAAGA TGGCTCATTT 
GACTACAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGATGGCACC 
ATCAAGCAGC AGGAGAGCGA TGACAGCCTG GTGGACTATG GCGAAGGCGG CGAGGGCCAG 
TTCAATGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA AAAAGGACAA GGAGGAAACG 
GAGGGCAATG AGAGCTCAGA GGCCACATCA CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006529644.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529707.2 Mus musculus neurofascin (Nfasc), transcript variant X13, mRNA.

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CloneID	OMu74375
Clone ID Related Accession (Same CDS sequence)	XM_006529644.2 , XM_006529644.3
Accession Version	XM_006529644.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529644.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529707.2
CDS	53..3946
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006529644.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529644.3

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248017.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246319.1 Mus musculus neurofascin (Nfasc), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu74376
Clone ID Related Accession (Same CDS sequence)	XM_011248017.2 , XM_011248017.1
Accession Version	XM_011248017.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTTAC ACCAATAACC AGGCAGACAT CGCCACCCAG 
GGCTGGTTCA TCGGGCTCAT GTGTGCCATT GCCCTTCTGG TGCTGATCCT TCTCATCGTC 
TGCTTCATCA AGAGGAGTCG AGGTGGCAAG TACCCAGTGC GGGAAAAGAA GGATGTCCCC 
TTGGGTCCTG AAGACCCCAA AGAAGAAGAT GGCTCATTTG ACTACAGTGA TGAGGACAAC 
AAGCCCCTGC AGGGCAGCCA GACATCTCTG GATGGCACCA TCAAGCAGCA GGAGAGCGAT 
GACAGCCTGG TGGACTATGG CGAAGGCGGC GAGGGCCAGT TCAATGAAGA TGGCTCCTTT 
ATTGGCCAGT ACACTGTCAA AAAGGACAAG GAGGAAACGG AGGGCAATGA GAGCTCAGAG 
GCCACATCAC CAGTCAATGC CATCTATTCC CTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246319.1
CDS	53..3931
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248017.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248017.2

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTTAC ACCAATAACC AGGCAGACAT CGCCACCCAG 
GGCTGGTTCA TCGGGCTCAT GTGTGCCATT GCCCTTCTGG TGCTGATCCT TCTCATCGTC 
TGCTTCATCA AGAGGAGTCG AGGTGGCAAG TACCCAGTGC GGGAAAAGAA GGATGTCCCC 
TTGGGTCCTG AAGACCCCAA AGAAGAAGAT GGCTCATTTG ACTACAGTGA TGAGGACAAC 
AAGCCCCTGC AGGGCAGCCA GACATCTCTG GATGGCACCA TCAAGCAGCA GGAGAGCGAT 
GACAGCCTGG TGGACTATGG CGAAGGCGGC GAGGGCCAGT TCAATGAAGA TGGCTCCTTT 
ATTGGCCAGT ACACTGTCAA AAAGGACAAG GAGGAAACGG AGGGCAATGA GAGCTCAGAG 
GCCACATCAC CAGTCAATGC CATCTATTCC CTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006529646.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529709.2 Mus musculus neurofascin (Nfasc), transcript variant X15, mRNA.

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CloneID	OMu74377
Clone ID Related Accession (Same CDS sequence)	XM_006529646.3 , XM_006529646.2
Accession Version	XM_006529646.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529646.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG CTGATCAGGC CACTCCAACT AACCGTTTGG CTGCCCTACC CAAAGGGCGA 
CCAGACCGAC CCAGGGACCT GGAGCTCACT GACCTGGCTG AGAGGAGTGT GAGGCTGACC 
TGGATCCCAG GGGATGACAA CAACAGCCCT ATCACAGACT ACGTCGTTCA GTTTGAAGAG 
GACCAGTTCC AACCAGGGGT GTGGCATGAC CACTCCAGGT TCCCAGGCAG CGTCAACTCA 
GCCGTCCTCC ATCTGTCCCC ATATGTCAAC TACCAATTCA GAGTCATCGC TGTCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTTCCATCC GAGCGGTACC GAACCAGTGG GGCACCCCCT 
GAATCTAATC CCAGTGATGT GAAGGGCGAA GGGACAAGAA AGAACAATAT GGAGATCACG 
TGGACGCCTA TGAATGCTAC CTCTGCCTTT GGCCCCAACC TACGCTACAT TGTCAAGTGG 
CGACGGAGAG AGACCCGAGA GACTTGGAAC AATGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CGCCTGTCTA CGTTCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTT 
GGGAAAGGCC CCGAGCCTGA CACCATCATT GGGTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAAT CCGAGTCCTG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGAGTCTACT CTGACACGGT CCAGGGCCAG CTCAGAGAGT ATCGAGCTTA CTACTGGAGG 
GAAAGCAGTT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAGGC CAGCTTCCCT 
GGTGACCGTC CCCGGGGCGT GGTGGCCCGC CTGTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTGGTGG TCAATGGGAG AGGTGACGGG CCTCGAAGTG AAACCAAGGA ATTCACCACC 
CCAGAAGGAG TACCCAGTGC CCCCAGGCGG TTCAGAGTCC GACAGCCCAA CCTGGAGACC 
ATCAACCTGG AGTGGGACCA CCCAGAGCAC CCCAACGGAA TCCTGATTGG ATACATCCTC 
AGATACGTGC CCTTTAATGG AACCAAACTG GGAAAGCAGA TGGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCTCTGT GCAGAGAGCA GACCCAGTGT CGCGTTACCG CTTCTCCCTC 
AGTGCCAGGA CACAGGTGGG CTCTGGAGAA GCAGCCACAG AGGAGTCCCC AGCACCTCCA 
AATGAAGCTA CTCCAACTGC AGCTTACACC AATAACCAGG CAGACATCGC CACCCAGGGC 
TGGTTCATCG GGCTCATGTG TGCCATTGCC CTTCTGGTGC TGATCCTTCT CATCGTCTGC 
TTCATCAAGA GGAGTCGAGG TGGCAAGTAC CCAGTGCGGG AAAAGAAGGA TGTCCCCTTG 
GGTCCTGAAG ACCCCAAAGA AGAAGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC ATCTCTGGAT GGCACCATCA AGCAGCAGGA GAGCGATGAC 
AGCCTGGTGG ACTATGGCGA AGGCGGCGAG GGCCAGTTCA ATGAAGATGG CTCCTTTATT 
GGCCAGTACA CTGTCAAAAA GGACAAGGAG GAAACGGAGG GCAATGAGAG CTCAGAGGCC 
ACATCACCAG TCAATGCCAT CTATTCCCTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529709.2
CDS	51..3926
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006529646.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529646.3

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG CTGATCAGGC CACTCCAACT AACCGTTTGG CTGCCCTACC CAAAGGGCGA 
CCAGACCGAC CCAGGGACCT GGAGCTCACT GACCTGGCTG AGAGGAGTGT GAGGCTGACC 
TGGATCCCAG GGGATGACAA CAACAGCCCT ATCACAGACT ACGTCGTTCA GTTTGAAGAG 
GACCAGTTCC AACCAGGGGT GTGGCATGAC CACTCCAGGT TCCCAGGCAG CGTCAACTCA 
GCCGTCCTCC ATCTGTCCCC ATATGTCAAC TACCAATTCA GAGTCATCGC TGTCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTTCCATCC GAGCGGTACC GAACCAGTGG GGCACCCCCT 
GAATCTAATC CCAGTGATGT GAAGGGCGAA GGGACAAGAA AGAACAATAT GGAGATCACG 
TGGACGCCTA TGAATGCTAC CTCTGCCTTT GGCCCCAACC TACGCTACAT TGTCAAGTGG 
CGACGGAGAG AGACCCGAGA GACTTGGAAC AATGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CGCCTGTCTA CGTTCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTT 
GGGAAAGGCC CCGAGCCTGA CACCATCATT GGGTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAAT CCGAGTCCTG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGAGTCTACT CTGACACGGT CCAGGGCCAG CTCAGAGAGT ATCGAGCTTA CTACTGGAGG 
GAAAGCAGTT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAGGC CAGCTTCCCT 
GGTGACCGTC CCCGGGGCGT GGTGGCCCGC CTGTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTGGTGG TCAATGGGAG AGGTGACGGG CCTCGAAGTG AAACCAAGGA ATTCACCACC 
CCAGAAGGAG TACCCAGTGC CCCCAGGCGG TTCAGAGTCC GACAGCCCAA CCTGGAGACC 
ATCAACCTGG AGTGGGACCA CCCAGAGCAC CCCAACGGAA TCCTGATTGG ATACATCCTC 
AGATACGTGC CCTTTAATGG AACCAAACTG GGAAAGCAGA TGGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCTCTGT GCAGAGAGCA GACCCAGTGT CGCGTTACCG CTTCTCCCTC 
AGTGCCAGGA CACAGGTGGG CTCTGGAGAA GCAGCCACAG AGGAGTCCCC AGCACCTCCA 
AATGAAGCTA CTCCAACTGC AGCTTACACC AATAACCAGG CAGACATCGC CACCCAGGGC 
TGGTTCATCG GGCTCATGTG TGCCATTGCC CTTCTGGTGC TGATCCTTCT CATCGTCTGC 
TTCATCAAGA GGAGTCGAGG TGGCAAGTAC CCAGTGCGGG AAAAGAAGGA TGTCCCCTTG 
GGTCCTGAAG ACCCCAAAGA AGAAGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC ATCTCTGGAT GGCACCATCA AGCAGCAGGA GAGCGATGAC 
AGCCTGGTGG ACTATGGCGA AGGCGGCGAG GGCCAGTTCA ATGAAGATGG CTCCTTTATT 
GGCCAGTACA CTGTCAAAAA GGACAAGGAG GAAACGGAGG GCAATGAGAG CTCAGAGGCC 
ACATCACCAG TCAATGCCAT CTATTCCCTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248018.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246320.1 Mus musculus neurofascin (Nfasc), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OMu74379
Clone ID Related Accession (Same CDS sequence)	XM_011248018.1 , XM_011248018.2
Accession Version	XM_011248018.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248018.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG GGCGACCAGA CCGACCCAGG GACCTGGAGC TCACTGACCT GGCTGAGAGG 
AGTGTGAGGC TGACCTGGAT CCCAGGGGAT GACAACAACA GCCCTATCAC AGACTACGTC 
GTTCAGTTTG AAGAGGACCA GTTCCAACCA GGGGTGTGGC ATGACCACTC CAGGTTCCCA 
GGCAGCGTCA ACTCAGCCGT CCTCCATCTG TCCCCATATG TCAACTACCA ATTCAGAGTC 
ATCGCTGTCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTTC CATCCGAGCG GTACCGAACC 
AGTGGGGCAC CCCCTGAATC TAATCCCAGT GATGTGAAGG GCGAAGGGAC AAGAAAGAAC 
AATATGGAGA TCACGTGGAC GCCTATGAAT GCTACCTCTG CCTTTGGCCC CAACCTACGC 
TACATTGTCA AGTGGCGACG GAGAGAGACC CGAGAGACTT GGAACAATGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACGCCT GTCTACGTTC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTTGGGAA AGGCCCCGAG CCTGACACCA TCATTGGGTA CTCCGGAGAA 
GATTATCCCA GGGCTGCGCC CACTGAAGTT AAAATCCGAG TCCTGAACAG CACAGCCATC 
AGCCTTCAGT GGAACCGAGT CTACTCTGAC ACGGTCCAGG GCCAGCTCAG AGAGTATCGA 
GCTTACTACT GGAGGGAAAG CAGTTTGCTG AAGAACCTGT GGGTGTCTCA GAAGAGACAG 
CAGGCCAGCT TCCCTGGTGA CCGTCCCCGG GGCGTGGTGG CCCGCCTGTT CCCCTACAGT 
AACTACAAGC TGGAGATGGT GGTGGTCAAT GGGAGAGGTG ACGGGCCTCG AAGTGAAACC 
AAGGAATTCA CCACCCCAGA AGGAGTACCC AGTGCCCCCA GGCGGTTCAG AGTCCGACAG 
CCCAACCTGG AGACCATCAA CCTGGAGTGG GACCACCCAG AGCACCCCAA CGGAATCCTG 
ATTGGATACA TCCTCAGATA CGTGCCCTTT AATGGAACCA AACTGGGAAA GCAGATGGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC TCTGTGCAGA GAGCAGACCC AGTGTCGCGT 
TACCGCTTCT CCCTCAGTGC CAGGACACAG GTGGGCTCTG GAGAAGCAGC CACAGAGGAG 
TCCCCAGCAC CTCCAAATGA AGCTACTCCA ACTGCAGCTT ACACCAATAA CCAGGCAGAC 
ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGTGCCA TTGCCCTTCT GGTGCTGATC 
CTTCTCATCG TCTGCTTCAT CAAGAGGAGT CGAGGTGGCA AGTACCCAGT GCGGGAAAAG 
AAGGATGTCC CCTTGGGTCC TGAAGACCCC AAAGAAGAAG ATGGCTCATT TGACTACAGT 
GATGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCTC TGGATGGCAC CATCAAGCAG 
CAGGAGAGCG ATGACAGCCT GGTGGACTAT GGCGAAGGCG GCGAGGGCCA GTTCAATGAA 
GATGGCTCCT TTATTGGCCA GTACACTGTC AAAAAGGACA AGGAGGAAAC GGAGGGCAAT 
GAGAGCTCAG AGGCCACATC ACCAGTCAAT GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246320.1
CDS	51..3881
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248018.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248018.2

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG GGCGACCAGA CCGACCCAGG GACCTGGAGC TCACTGACCT GGCTGAGAGG 
AGTGTGAGGC TGACCTGGAT CCCAGGGGAT GACAACAACA GCCCTATCAC AGACTACGTC 
GTTCAGTTTG AAGAGGACCA GTTCCAACCA GGGGTGTGGC ATGACCACTC CAGGTTCCCA 
GGCAGCGTCA ACTCAGCCGT CCTCCATCTG TCCCCATATG TCAACTACCA ATTCAGAGTC 
ATCGCTGTCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTTC CATCCGAGCG GTACCGAACC 
AGTGGGGCAC CCCCTGAATC TAATCCCAGT GATGTGAAGG GCGAAGGGAC AAGAAAGAAC 
AATATGGAGA TCACGTGGAC GCCTATGAAT GCTACCTCTG CCTTTGGCCC CAACCTACGC 
TACATTGTCA AGTGGCGACG GAGAGAGACC CGAGAGACTT GGAACAATGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACGCCT GTCTACGTTC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTTGGGAA AGGCCCCGAG CCTGACACCA TCATTGGGTA CTCCGGAGAA 
GATTATCCCA GGGCTGCGCC CACTGAAGTT AAAATCCGAG TCCTGAACAG CACAGCCATC 
AGCCTTCAGT GGAACCGAGT CTACTCTGAC ACGGTCCAGG GCCAGCTCAG AGAGTATCGA 
GCTTACTACT GGAGGGAAAG CAGTTTGCTG AAGAACCTGT GGGTGTCTCA GAAGAGACAG 
CAGGCCAGCT TCCCTGGTGA CCGTCCCCGG GGCGTGGTGG CCCGCCTGTT CCCCTACAGT 
AACTACAAGC TGGAGATGGT GGTGGTCAAT GGGAGAGGTG ACGGGCCTCG AAGTGAAACC 
AAGGAATTCA CCACCCCAGA AGGAGTACCC AGTGCCCCCA GGCGGTTCAG AGTCCGACAG 
CCCAACCTGG AGACCATCAA CCTGGAGTGG GACCACCCAG AGCACCCCAA CGGAATCCTG 
ATTGGATACA TCCTCAGATA CGTGCCCTTT AATGGAACCA AACTGGGAAA GCAGATGGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC TCTGTGCAGA GAGCAGACCC AGTGTCGCGT 
TACCGCTTCT CCCTCAGTGC CAGGACACAG GTGGGCTCTG GAGAAGCAGC CACAGAGGAG 
TCCCCAGCAC CTCCAAATGA AGCTACTCCA ACTGCAGCTT ACACCAATAA CCAGGCAGAC 
ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGTGCCA TTGCCCTTCT GGTGCTGATC 
CTTCTCATCG TCTGCTTCAT CAAGAGGAGT CGAGGTGGCA AGTACCCAGT GCGGGAAAAG 
AAGGATGTCC CCTTGGGTCC TGAAGACCCC AAAGAAGAAG ATGGCTCATT TGACTACAGT 
GATGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCTC TGGATGGCAC CATCAAGCAG 
CAGGAGAGCG ATGACAGCCT GGTGGACTAT GGCGAAGGCG GCGAGGGCCA GTTCAATGAA 
GATGGCTCCT TTATTGGCCA GTACACTGTC AAAAAGGACA AGGAGGAAAC GGAGGGCAAT 
GAGAGCTCAG AGGCCACATC ACCAGTCAAT GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006529651.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529714.2 Mus musculus neurofascin (Nfasc), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OMu74380
Clone ID Related Accession (Same CDS sequence)	XM_006529651.2 , XM_006529651.3
Accession Version	XM_006529651.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529651.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG GGCGACCAGA CCGACCCAGG GACCTGGAGC TCACTGACCT GGCTGAGAGG 
AGTGTGAGGC TGACCTGGAT CCCAGGGGAT GACAACAACA GCCCTATCAC AGACTACGTC 
GTTCAGTTTG AAGAGGACCA GTTCCAACCA GGGGTGTGGC ATGACCACTC CAGGTTCCCA 
GGCAGCGTCA ACTCAGCCGT CCTCCATCTG TCCCCATATG TCAACTACCA ATTCAGAGTC 
ATCGCTGTCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTTC CATCCGAGCG GTACCGAACC 
AGTGGGGCAC CCCCTGAATC TAATCCCAGT GATGTGAAGG GCGAAGGGAC AAGAAAGAAC 
AATATGGAGA TCACGTGGAC GCCTATGAAT GCTACCTCTG CCTTTGGCCC CAACCTACGC 
TACATTGTCA AGTGGCGACG GAGAGAGACC CGAGAGACTT GGAACAATGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACGCCT GTCTACGTTC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTTGGGAA AGGCCCCGAG CCTGACACCA TCATTGGGTA CTCCGGAGAA 
GATTATCCCA GGGCTGCGCC CACTGAAGTT AAAATCCGAG TCCTGAACAG CACAGCCATC 
AGCCTTCAGT GGAACCGAGT CTACTCTGAC ACGGTCCAGG GCCAGCTCAG AGAGTATCGA 
GCTTACTACT GGAGGGAAAG CAGTTTGCTG AAGAACCTGT GGGTGTCTCA GAAGAGACAG 
CAGGCCAGCT TCCCTGGTGA CCGTCCCCGG GGCGTGGTGG CCCGCCTGTT CCCCTACAGT 
AACTACAAGC TGGAGATGGT GGTGGTCAAT GGGAGAGGTG ACGGGCCTCG AAGTGAAACC 
AAGGAATTCA CCACCCCAGA AGGAGTACCC AGTGCCCCCA GGCGGTTCAG AGTCCGACAG 
CCCAACCTGG AGACCATCAA CCTGGAGTGG GACCACCCAG AGCACCCCAA CGGAATCCTG 
ATTGGATACA TCCTCAGATA CGTGCCCTTT AATGGAACCA AACTGGGAAA GCAGATGGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC TCTGTGCAGA GAGCAGACCC AGTGTCGCGT 
TACCGCTTCT CCCTCAGTGC CAGGACACAG GTGGGCTCTG GAGAAGCAGC CACAGAGGAG 
TCCCCAGCAC CTCCAAATGA AGCTTACACC AATAACCAGG CAGACATCGC CACCCAGGGC 
TGGTTCATCG GGCTCATGTG TGCCATTGCC CTTCTGGTGC TGATCCTTCT CATCGTCTGC 
TTCATCAAGA GGAGTCGAGG TGGCAAGTAC CCAGTGCGGG AAAAGAAGGA TGTCCCCTTG 
GGTCCTGAAG ACCCCAAAGA AGAAGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC ATCTCTGGAT GGCACCATCA AGCAGCAGGA GAGCGATGAC 
AGCCTGGTGG ACTATGGCGA AGGCGGCGAG GGCCAGTTCA ATGAAGATGG CTCCTTTATT 
GGCCAGTACA CTGTCAAAAA GGACAAGGAG GAAACGGAGG GCAATGAGAG CTCAGAGGCC 
ACATCACCAG TCAATGCCAT CTATTCCCTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529714.2
CDS	51..3866
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006529651.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529651.3

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG GGCGACCAGA CCGACCCAGG GACCTGGAGC TCACTGACCT GGCTGAGAGG 
AGTGTGAGGC TGACCTGGAT CCCAGGGGAT GACAACAACA GCCCTATCAC AGACTACGTC 
GTTCAGTTTG AAGAGGACCA GTTCCAACCA GGGGTGTGGC ATGACCACTC CAGGTTCCCA 
GGCAGCGTCA ACTCAGCCGT CCTCCATCTG TCCCCATATG TCAACTACCA ATTCAGAGTC 
ATCGCTGTCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTTC CATCCGAGCG GTACCGAACC 
AGTGGGGCAC CCCCTGAATC TAATCCCAGT GATGTGAAGG GCGAAGGGAC AAGAAAGAAC 
AATATGGAGA TCACGTGGAC GCCTATGAAT GCTACCTCTG CCTTTGGCCC CAACCTACGC 
TACATTGTCA AGTGGCGACG GAGAGAGACC CGAGAGACTT GGAACAATGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACGCCT GTCTACGTTC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTTGGGAA AGGCCCCGAG CCTGACACCA TCATTGGGTA CTCCGGAGAA 
GATTATCCCA GGGCTGCGCC CACTGAAGTT AAAATCCGAG TCCTGAACAG CACAGCCATC 
AGCCTTCAGT GGAACCGAGT CTACTCTGAC ACGGTCCAGG GCCAGCTCAG AGAGTATCGA 
GCTTACTACT GGAGGGAAAG CAGTTTGCTG AAGAACCTGT GGGTGTCTCA GAAGAGACAG 
CAGGCCAGCT TCCCTGGTGA CCGTCCCCGG GGCGTGGTGG CCCGCCTGTT CCCCTACAGT 
AACTACAAGC TGGAGATGGT GGTGGTCAAT GGGAGAGGTG ACGGGCCTCG AAGTGAAACC 
AAGGAATTCA CCACCCCAGA AGGAGTACCC AGTGCCCCCA GGCGGTTCAG AGTCCGACAG 
CCCAACCTGG AGACCATCAA CCTGGAGTGG GACCACCCAG AGCACCCCAA CGGAATCCTG 
ATTGGATACA TCCTCAGATA CGTGCCCTTT AATGGAACCA AACTGGGAAA GCAGATGGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC TCTGTGCAGA GAGCAGACCC AGTGTCGCGT 
TACCGCTTCT CCCTCAGTGC CAGGACACAG GTGGGCTCTG GAGAAGCAGC CACAGAGGAG 
TCCCCAGCAC CTCCAAATGA AGCTTACACC AATAACCAGG CAGACATCGC CACCCAGGGC 
TGGTTCATCG GGCTCATGTG TGCCATTGCC CTTCTGGTGC TGATCCTTCT CATCGTCTGC 
TTCATCAAGA GGAGTCGAGG TGGCAAGTAC CCAGTGCGGG AAAAGAAGGA TGTCCCCTTG 
GGTCCTGAAG ACCCCAAAGA AGAAGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC ATCTCTGGAT GGCACCATCA AGCAGCAGGA GAGCGATGAC 
AGCCTGGTGG ACTATGGCGA AGGCGGCGAG GGCCAGTTCA ATGAAGATGG CTCCTTTATT 
GGCCAGTACA CTGTCAAAAA GGACAAGGAG GAAACGGAGG GCAATGAGAG CTCAGAGGCC 
ACATCACCAG TCAATGCCAT CTATTCCCTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006529653.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529716.2 Mus musculus neurofascin (Nfasc), transcript variant X20, mRNA.

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CloneID	OMu74381
Clone ID Related Accession (Same CDS sequence)	XM_006529653.4 , XM_006529653.3
Accession Version	XM_006529653.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529653.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCAGA AAGAACGCCC 
AGCTTCATGT ATCCCCAGGG CACATCGAGC AGTCAGATGG TTCTCCGTGG CATGGACCTG 
CTGCTGGAAT GCATTGCCTC TGGCGTCCCA ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTAACAA GGCCAAGTTC GAGAACTTTA ATAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCAT ACTGGCTGGA TGAGCCCAAG 
AACCTGATCC TGGCTCCTGG GGAAGATGGG AGGCTGGTAT GCCGAGCCAA TGGGAACCCG 
AAGCCGACCG TGCAGTGGAT GGTGAATGGA GAGCCTTTAC AATCGGCACC ACCCAATCCC 
AACCGTGAGG TAGCTGGAGA CACTATCATC TTCCGGGATA CTCAGATCAG CAGCAGGGCA 
GTGTACCAAT GTAATACATC CAATGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTGTTAGATG TACCCCCTCG GATGCTGTCT GCCCGCAACC AGCTCATCAG GGTGATCCTT 
TATAACCGGA CACGGCTGGA CTGTCCGTTC TTTGGGTCTC CCATCCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGCGGTAACT ACCACGTCTA CGAAAACGGC 
AGTCTAGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GCAAAGCCGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CCGAGGACCA GGTGGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAACATG ACCCCTCCTT GAAGCTCACA GTCTCCTGGC TGAAGGACGA TGAGCCACTC 
TACATTGGAA ACAGGATGAA GAAGGAAGAT GACTCCCTGA CGATCTTCGG AGTGGCAGAG 
CGGGACCAGG GCAGTTACAC GTGTATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGG GCGACCAGAC CGACCCAGGG ACCTGGAGCT CACTGACCTG 
GCTGAGAGGA GTGTGAGGCT GACCTGGATC CCAGGGGATG ACAACAACAG CCCTATCACA 
GACTACGTCG TTCAGTTTGA AGAGGACCAG TTCCAACCAG GGGTGTGGCA TGACCACTCC 
AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC CTCCATCTGT CCCCATATGT CAACTACCAA 
TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG AGCAGCCACC CCAGCCTTCC ATCCGAGCGG 
TACCGAACCA GTGGGGCACC CCCTGAATCT AATCCCAGTG ATGTGAAGGG CGAAGGGACA 
AGAAAGAACA ATATGGAGAT CACGTGGACG CCTATGAATG CTACCTCTGC CTTTGGCCCC 
AACCTACGCT ACATTGTCAA GTGGCGACGG AGAGAGACCC GAGAGACTTG GAACAATGTC 
ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG CAGACGCCTG TCTACGTTCC CTATGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAA GGCCCCGAGC CTGACACCAT CATTGGGTAC 
TCCGGAGAAG ATTATCCCAG GGCTGCGCCC ACTGAAGTTA AAATCCGAGT CCTGAACAGC 
ACAGCCATCA GCCTTCAGTG GAACCGAGTC TACTCTGACA CGGTCCAGGG CCAGCTCAGA 
GAGTATCGAG CTTACTACTG GAGGGAAAGC AGTTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGACAGC AGGCCAGCTT CCCTGGTGAC CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTG GTGGTCAATG GGAGAGGTGA CGGGCCTCGA 
AGTGAAACCA AGGAATTCAC CACCCCAGAA GGAGTACCCA GTGCCCCCAG GCGGTTCAGA 
GTCCGACAGC CCAACCTGGA GACCATCAAC CTGGAGTGGG ACCACCCAGA GCACCCCAAC 
GGAATCCTGA TTGGATACAT CCTCAGATAC GTGCCCTTTA ATGGAACCAA ACTGGGAAAG 
CAGATGGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCT CTGTGCAGAG AGCAGACCCA 
GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC AGGACACAGG TGGGCTCTGG AGAAGCAGCC 
ACAGAGGAGT CCCCAGCACC TCCAAATGAA GCTACTCCAA CTGCAGCTTA CACCAATAAC 
CAGGCAGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG 
GTGCTGATCC TTCTCATCGT CTGCTTCATC AAGAGGAGTC GAGGTGGCAA GTACCCAGTG 
CGGGAAAAGA AGGATGTCCC CTTGGGTCCT GAAGACCCCA AAGAAGAAGA TGGCTCATTT 
GACTACAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGATGGCACC 
ATCAAGCAGC AGGAGAGCGA TGACAGCCTG GTGGACTATG GCGAAGGCGG CGAGGGCCAG 
TTCAATGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA AAAAGGACAA GGAGGAAACG 
GAGGGCAATG AGAGCTCAGA GGCCACATCA CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529716.2
CDS	51..3830
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006529653.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529653.4

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCAGA AAGAACGCCC 
AGCTTCATGT ATCCCCAGGG CACATCGAGC AGTCAGATGG TTCTCCGTGG CATGGACCTG 
CTGCTGGAAT GCATTGCCTC TGGCGTCCCA ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTAACAA GGCCAAGTTC GAGAACTTTA ATAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCAT ACTGGCTGGA TGAGCCCAAG 
AACCTGATCC TGGCTCCTGG GGAAGATGGG AGGCTGGTAT GCCGAGCCAA TGGGAACCCG 
AAGCCGACCG TGCAGTGGAT GGTGAATGGA GAGCCTTTAC AATCGGCACC ACCCAATCCC 
AACCGTGAGG TAGCTGGAGA CACTATCATC TTCCGGGATA CTCAGATCAG CAGCAGGGCA 
GTGTACCAAT GTAATACATC CAATGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTGTTAGATG TACCCCCTCG GATGCTGTCT GCCCGCAACC AGCTCATCAG GGTGATCCTT 
TATAACCGGA CACGGCTGGA CTGTCCGTTC TTTGGGTCTC CCATCCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGCGGTAACT ACCACGTCTA CGAAAACGGC 
AGTCTAGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GCAAAGCCGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CCGAGGACCA GGTGGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAACATG ACCCCTCCTT GAAGCTCACA GTCTCCTGGC TGAAGGACGA TGAGCCACTC 
TACATTGGAA ACAGGATGAA GAAGGAAGAT GACTCCCTGA CGATCTTCGG AGTGGCAGAG 
CGGGACCAGG GCAGTTACAC GTGTATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGG GCGACCAGAC CGACCCAGGG ACCTGGAGCT CACTGACCTG 
GCTGAGAGGA GTGTGAGGCT GACCTGGATC CCAGGGGATG ACAACAACAG CCCTATCACA 
GACTACGTCG TTCAGTTTGA AGAGGACCAG TTCCAACCAG GGGTGTGGCA TGACCACTCC 
AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC CTCCATCTGT CCCCATATGT CAACTACCAA 
TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG AGCAGCCACC CCAGCCTTCC ATCCGAGCGG 
TACCGAACCA GTGGGGCACC CCCTGAATCT AATCCCAGTG ATGTGAAGGG CGAAGGGACA 
AGAAAGAACA ATATGGAGAT CACGTGGACG CCTATGAATG CTACCTCTGC CTTTGGCCCC 
AACCTACGCT ACATTGTCAA GTGGCGACGG AGAGAGACCC GAGAGACTTG GAACAATGTC 
ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG CAGACGCCTG TCTACGTTCC CTATGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAA GGCCCCGAGC CTGACACCAT CATTGGGTAC 
TCCGGAGAAG ATTATCCCAG GGCTGCGCCC ACTGAAGTTA AAATCCGAGT CCTGAACAGC 
ACAGCCATCA GCCTTCAGTG GAACCGAGTC TACTCTGACA CGGTCCAGGG CCAGCTCAGA 
GAGTATCGAG CTTACTACTG GAGGGAAAGC AGTTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGACAGC AGGCCAGCTT CCCTGGTGAC CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTG GTGGTCAATG GGAGAGGTGA CGGGCCTCGA 
AGTGAAACCA AGGAATTCAC CACCCCAGAA GGAGTACCCA GTGCCCCCAG GCGGTTCAGA 
GTCCGACAGC CCAACCTGGA GACCATCAAC CTGGAGTGGG ACCACCCAGA GCACCCCAAC 
GGAATCCTGA TTGGATACAT CCTCAGATAC GTGCCCTTTA ATGGAACCAA ACTGGGAAAG 
CAGATGGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCT CTGTGCAGAG AGCAGACCCA 
GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC AGGACACAGG TGGGCTCTGG AGAAGCAGCC 
ACAGAGGAGT CCCCAGCACC TCCAAATGAA GCTACTCCAA CTGCAGCTTA CACCAATAAC 
CAGGCAGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG 
GTGCTGATCC TTCTCATCGT CTGCTTCATC AAGAGGAGTC GAGGTGGCAA GTACCCAGTG 
CGGGAAAAGA AGGATGTCCC CTTGGGTCCT GAAGACCCCA AAGAAGAAGA TGGCTCATTT 
GACTACAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGATGGCACC 
ATCAAGCAGC AGGAGAGCGA TGACAGCCTG GTGGACTATG GCGAAGGCGG CGAGGGCCAG 
TTCAATGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA AAAAGGACAA GGAGGAAACG 
GAGGGCAATG AGAGCTCAGA GGCCACATCA CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248024.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246326.1 Mus musculus neurofascin (Nfasc), transcript variant X22, mRNA.

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CloneID	OMu74382
Clone ID Related Accession (Same CDS sequence)	XM_011248024.2 , XM_011248023.1 , XM_011248019.1 , XM_011248024.3
Accession Version	XM_011248024.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248024.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACTATCA CCAAGCAGTC CGTGAAGGAC CACATCGTGG ACCCTCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG CAACCCCGCC CCCAGTTTTC ACTGGACTCG CAACAGCAGA 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGATCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAATACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TTGGCACTGC ACTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTGACACTA 
CAGTGCAACC CCCCACCCGG CCTCCCGTCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ACGCGCGCTT TCACTTCACC 
CACACCATTC AGCAGAAGAA CCCCTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC AGAAAGAACG 
CCCAGCTTCA TGTATCCCCA GGGCACATCG AGCAGTCAGA TGGTTCTCCG TGGCATGGAC 
CTGCTGCTGG AATGCATTGC CTCTGGCGTC CCAACACCAG ACATTGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTAA CAAGGCCAAG TTCGAGAACT TTAATAAGGC TCTGCGCATC 
ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CATACTGGCT GGATGAGCCC 
AAGAACCTGA TCCTGGCTCC TGGGGAAGAT GGGAGGCTGG TATGCCGAGC CAATGGGAAC 
CCGAAGCCGA CCGTGCAGTG GATGGTGAAT GGAGAGCCTT TACAATCGGC ACCACCCAAT 
CCCAACCGTG AGGTAGCTGG AGACACTATC ATCTTCCGGG ATACTCAGAT CAGCAGCAGG 
GCAGTGTACC AATGTAATAC ATCCAATGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC 
AGCGTGTTAG ATGTACCCCC TCGGATGCTG TCTGCCCGCA ACCAGCTCAT CAGGGTGATC 
CTTTATAACC GGACACGGCT GGACTGTCCG TTCTTTGGGT CTCCCATCCC AACACTCCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGCGGTA ACTACCACGT CTACGAAAAC 
GGCAGTCTAG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC 
ACCAACATCC TGGGCAAAGC CGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACAGGA TGCCCGAGGA CCAGGTGGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC 
CGCGTGAAAC ATGACCCCTC CTTGAAGCTC ACAGTCTCCT GGCTGAAGGA CGATGAGCCA 
CTCTACATTG GAAACAGGAT GAAGAAGGAA GATGACTCCC TGACGATCTT CGGAGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTATG GCCAGCACCG AGCTGGACCA GGACCTGGCA 
AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTA 
CCCAAAGGGC GACCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT 
GTGAGGCTGA CCTGGATCCC AGGGGATGAC AACAACAGCC CTATCACAGA CTACGTCGTT 
CAGTTTGAAG AGGACCAGTT CCAACCAGGG GTGTGGCATG ACCACTCCAG GTTCCCAGGC 
AGCGTCAACT CAGCCGTCCT CCATCTGTCC CCATATGTCA ACTACCAATT CAGAGTCATC 
GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACCAGT 
GGGGCACCCC CTGAATCTAA TCCCAGTGAT GTGAAGGGCG AAGGGACAAG AAAGAACAAT 
ATGGAGATCA CGTGGACGCC TATGAATGCT ACCTCTGCCT TTGGCCCCAA CCTACGCTAC 
ATTGTCAAGT GGCGACGGAG AGAGACCCGA GAGACTTGGA ACAATGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACGCCTGTC TACGTTCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TTGGGAAAGG CCCCGAGCCT GACACCATCA TTGGGTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGAGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT 
TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG 
GCCAGCTTCC CTGGTGACCG TCCCCGGGGC GTGGTGGCCC GCCTGTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTGGT GGTCAATGGG AGAGGTGACG GGCCTCGAAG TGAAACCAAG 
GAATTCACCA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GGTTCAGAGT CCGACAGCCC 
AACCTGGAGA CCATCAACCT GGAGTGGGAC CACCCAGAGC ACCCCAACGG AATCCTGATT 
GGATACATCC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCTCT GTGCAGAGAG CAGACCCAGT GTCGCGTTAC 
CGCTTCTCCC TCAGTGCCAG GACACAGGTG GGCTCTGGAG AAGCAGCCAC AGAGGAGTCC 
CCAGCACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACGTTGCC CCCGACTACT 
GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA 
GCCACAACAG TTCCCATCAT TCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC 
ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CTACGGAGAG CCCTCCCACT 
ACCACTGCTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC 
ACAGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA 
ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG 
GCCCAGGCCC AGCCTATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG 
GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA 
GCTTACACCA ATAACCAGGC AGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT 
GCCATTGCCC TTCTGGTGCT GATCCTTCTC ATCGTCTGCT TCATCAAGAG GAGTCGAGGT 
GGCAAGTACC CAGTGCGGGA AAAGAAGGAT GTCCCCTTGG GTCCTGAAGA CCCCAAAGAA 
GAAGATGGCT CATTTGACTA CAGTGATGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA 
TCTCTGGATG GCACCATCAA GCAGCAGGAG AGCGATGACA GCCTGGTGGA CTATGGCGAA 
GGCGGCGAGG GCCAGTTCAA TGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAAAAAG 
GACAAGGAGG AAACGGAGGG CAATGAGAGC TCAGAGGCCA CATCACCAGT CAATGCCATC 
TATTCCCTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246326.1
CDS	161..4255
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248024.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248024.3

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACTATCA CCAAGCAGTC CGTGAAGGAC CACATCGTGG ACCCTCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG CAACCCCGCC CCCAGTTTTC ACTGGACTCG CAACAGCAGA 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGATCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAATACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TTGGCACTGC ACTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTGACACTA 
CAGTGCAACC CCCCACCCGG CCTCCCGTCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ACGCGCGCTT TCACTTCACC 
CACACCATTC AGCAGAAGAA CCCCTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC AGAAAGAACG 
CCCAGCTTCA TGTATCCCCA GGGCACATCG AGCAGTCAGA TGGTTCTCCG TGGCATGGAC 
CTGCTGCTGG AATGCATTGC CTCTGGCGTC CCAACACCAG ACATTGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTAA CAAGGCCAAG TTCGAGAACT TTAATAAGGC TCTGCGCATC 
ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CATACTGGCT GGATGAGCCC 
AAGAACCTGA TCCTGGCTCC TGGGGAAGAT GGGAGGCTGG TATGCCGAGC CAATGGGAAC 
CCGAAGCCGA CCGTGCAGTG GATGGTGAAT GGAGAGCCTT TACAATCGGC ACCACCCAAT 
CCCAACCGTG AGGTAGCTGG AGACACTATC ATCTTCCGGG ATACTCAGAT CAGCAGCAGG 
GCAGTGTACC AATGTAATAC ATCCAATGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC 
AGCGTGTTAG ATGTACCCCC TCGGATGCTG TCTGCCCGCA ACCAGCTCAT CAGGGTGATC 
CTTTATAACC GGACACGGCT GGACTGTCCG TTCTTTGGGT CTCCCATCCC AACACTCCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGCGGTA ACTACCACGT CTACGAAAAC 
GGCAGTCTAG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC 
ACCAACATCC TGGGCAAAGC CGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACAGGA TGCCCGAGGA CCAGGTGGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC 
CGCGTGAAAC ATGACCCCTC CTTGAAGCTC ACAGTCTCCT GGCTGAAGGA CGATGAGCCA 
CTCTACATTG GAAACAGGAT GAAGAAGGAA GATGACTCCC TGACGATCTT CGGAGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTATG GCCAGCACCG AGCTGGACCA GGACCTGGCA 
AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTA 
CCCAAAGGGC GACCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT 
GTGAGGCTGA CCTGGATCCC AGGGGATGAC AACAACAGCC CTATCACAGA CTACGTCGTT 
CAGTTTGAAG AGGACCAGTT CCAACCAGGG GTGTGGCATG ACCACTCCAG GTTCCCAGGC 
AGCGTCAACT CAGCCGTCCT CCATCTGTCC CCATATGTCA ACTACCAATT CAGAGTCATC 
GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACCAGT 
GGGGCACCCC CTGAATCTAA TCCCAGTGAT GTGAAGGGCG AAGGGACAAG AAAGAACAAT 
ATGGAGATCA CGTGGACGCC TATGAATGCT ACCTCTGCCT TTGGCCCCAA CCTACGCTAC 
ATTGTCAAGT GGCGACGGAG AGAGACCCGA GAGACTTGGA ACAATGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACGCCTGTC TACGTTCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TTGGGAAAGG CCCCGAGCCT GACACCATCA TTGGGTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGAGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT 
TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG 
GCCAGCTTCC CTGGTGACCG TCCCCGGGGC GTGGTGGCCC GCCTGTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTGGT GGTCAATGGG AGAGGTGACG GGCCTCGAAG TGAAACCAAG 
GAATTCACCA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GGTTCAGAGT CCGACAGCCC 
AACCTGGAGA CCATCAACCT GGAGTGGGAC CACCCAGAGC ACCCCAACGG AATCCTGATT 
GGATACATCC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCTCT GTGCAGAGAG CAGACCCAGT GTCGCGTTAC 
CGCTTCTCCC TCAGTGCCAG GACACAGGTG GGCTCTGGAG AAGCAGCCAC AGAGGAGTCC 
CCAGCACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACGTTGCC CCCGACTACT 
GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA 
GCCACAACAG TTCCCATCAT TCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC 
ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CTACGGAGAG CCCTCCCACT 
ACCACTGCTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC 
ACAGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA 
ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG 
GCCCAGGCCC AGCCTATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG 
GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA 
GCTTACACCA ATAACCAGGC AGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT 
GCCATTGCCC TTCTGGTGCT GATCCTTCTC ATCGTCTGCT TCATCAAGAG GAGTCGAGGT 
GGCAAGTACC CAGTGCGGGA AAAGAAGGAT GTCCCCTTGG GTCCTGAAGA CCCCAAAGAA 
GAAGATGGCT CATTTGACTA CAGTGATGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA 
TCTCTGGATG GCACCATCAA GCAGCAGGAG AGCGATGACA GCCTGGTGGA CTATGGCGAA 
GGCGGCGAGG GCCAGTTCAA TGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAAAAAG 
GACAAGGAGG AAACGGAGGG CAATGAGAGC TCAGAGGCCA CATCACCAGT CAATGCCATC 
TATTCCCTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_030254547.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110407.1 Mus musculus neurofascin (Nfasc), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OMu74383
Clone ID Related Accession (Same CDS sequence)	XM_011248020.2 , XM_011248021.1 , XM_030254547.1 , XM_011248020.3
Accession Version	XM_030254547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TACCCAAAGG GCGACCAGAC 
CGACCCAGGG ACCTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC 
CCAGGGGATG ACAACAACAG CCCTATCACA GACTACGTCG TTCAGTTTGA AGAGGACCAG 
TTCCAACCAG GGGTGTGGCA TGACCACTCC AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC 
CTCCATCTGT CCCCATATGT CAACTACCAA TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTTCC ATCCGAGCGG TACCGAACCA GTGGGGCACC CCCTGAATCT 
AATCCCAGTG ATGTGAAGGG CGAAGGGACA AGAAAGAACA ATATGGAGAT CACGTGGACG 
CCTATGAATG CTACCTCTGC CTTTGGCCCC AACCTACGCT ACATTGTCAA GTGGCGACGG 
AGAGAGACCC GAGAGACTTG GAACAATGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACGCCTG TCTACGTTCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTTGGGAAA 
GGCCCCGAGC CTGACACCAT CATTGGGTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGAGTC 
TACTCTGACA CGGTCCAGGG CCAGCTCAGA GAGTATCGAG CTTACTACTG GAGGGAAAGC 
AGTTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AGGCCAGCTT CCCTGGTGAC 
CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC CCCTACAGTA ACTACAAGCT GGAGATGGTG 
GTGGTCAATG GGAGAGGTGA CGGGCCTCGA AGTGAAACCA AGGAATTCAC CACCCCAGAA 
GGAGTACCCA GTGCCCCCAG GCGGTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC 
CTGGAGTGGG ACCACCCAGA GCACCCCAAC GGAATCCTGA TTGGATACAT CCTCAGATAC 
GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCT CTGTGCAGAG AGCAGACCCA GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC 
AGGACACAGG TGGGCTCTGG AGAAGCAGCC ACAGAGGAGT CCCCAGCACC TCCAAATGAA 
GCTACTCCAA CTGCAGCTCC TCCCACGTTG CCCCCGACTA CTGTGGGTAC CACAGGCCTT 
GTGAGCAGTA CTGATGCTAC TGCCCTTGCT GCCACCAGTG AAGCCACAAC AGTTCCCATC 
ATTCCAACCG TCGTACCTAC CACCGTCGCC ACCACCATTG CCACAACTAC TACAACCACT 
GCCGCCACCA CCACCACCAC CACTACGGAG AGCCCTCCCA CTACCACTGC TGGGACTAAG 
ATTCACGAAA CCGCCCCCGA CGAGCAGTCC ATTTGGAACG TCACAGTGCT CCCCAACAGT 
AAATGGGCCA ACATCACCTG GAAGCACAAT TTCAGGCCTG GAACTGACTT TGTGGTTGAG 
TACATCGACA GCAACCATAC GAAAAAAACT GTCCCTGTTA AGGCCCAGGC CCAGCCTATA 
CAGCTGACAG ACCTCTTTCC CGGGATGACG TACACGTTGC GGGTGTATTC CCGGGACAAC 
GAGGGCATCA GCAGTACCGT CATCACCTTT ATGACCAGTA CAGCTTACAC CAATAACCAG 
GCAGACATCG CCACCCAGGG CTGGTTCATC GGGCTCATGT GTGCCATTGC CCTTCTGGTG 
CTGATCCTTC TCATCGTCTG CTTCATCAAG AGGAGTCGAG GTGGCAAGTA CCCAGTGCGG 
GAAAAGAAGG ATGTCCCCTT GGGTCCTGAA GACCCCAAAG AAGAAGATGG CTCATTTGAC 
TACAGTGATG AGGACAACAA GCCCCTGCAG GGCAGCCAGA CATCTCTGGA TGGCACCATC 
AAGCAGCAGG AGAGCGATGA CAGCCTGGTG GACTATGGCG AAGGCGGCGA GGGCCAGTTC 
AATGAAGATG GCTCCTTTAT TGGCCAGTAC ACTGTCAAAA AGGACAAGGA GGAAACGGAG 
GGCAATGAGA GCTCAGAGGC CACATCACCA GTCAATGCCA TCTATTCCCT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110407.1
CDS	312..4388
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_030254547.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254547.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TACCCAAAGG GCGACCAGAC 
CGACCCAGGG ACCTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC 
CCAGGGGATG ACAACAACAG CCCTATCACA GACTACGTCG TTCAGTTTGA AGAGGACCAG 
TTCCAACCAG GGGTGTGGCA TGACCACTCC AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC 
CTCCATCTGT CCCCATATGT CAACTACCAA TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTTCC ATCCGAGCGG TACCGAACCA GTGGGGCACC CCCTGAATCT 
AATCCCAGTG ATGTGAAGGG CGAAGGGACA AGAAAGAACA ATATGGAGAT CACGTGGACG 
CCTATGAATG CTACCTCTGC CTTTGGCCCC AACCTACGCT ACATTGTCAA GTGGCGACGG 
AGAGAGACCC GAGAGACTTG GAACAATGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACGCCTG TCTACGTTCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTTGGGAAA 
GGCCCCGAGC CTGACACCAT CATTGGGTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGAGTC 
TACTCTGACA CGGTCCAGGG CCAGCTCAGA GAGTATCGAG CTTACTACTG GAGGGAAAGC 
AGTTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AGGCCAGCTT CCCTGGTGAC 
CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC CCCTACAGTA ACTACAAGCT GGAGATGGTG 
GTGGTCAATG GGAGAGGTGA CGGGCCTCGA AGTGAAACCA AGGAATTCAC CACCCCAGAA 
GGAGTACCCA GTGCCCCCAG GCGGTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC 
CTGGAGTGGG ACCACCCAGA GCACCCCAAC GGAATCCTGA TTGGATACAT CCTCAGATAC 
GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCT CTGTGCAGAG AGCAGACCCA GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC 
AGGACACAGG TGGGCTCTGG AGAAGCAGCC ACAGAGGAGT CCCCAGCACC TCCAAATGAA 
GCTACTCCAA CTGCAGCTCC TCCCACGTTG CCCCCGACTA CTGTGGGTAC CACAGGCCTT 
GTGAGCAGTA CTGATGCTAC TGCCCTTGCT GCCACCAGTG AAGCCACAAC AGTTCCCATC 
ATTCCAACCG TCGTACCTAC CACCGTCGCC ACCACCATTG CCACAACTAC TACAACCACT 
GCCGCCACCA CCACCACCAC CACTACGGAG AGCCCTCCCA CTACCACTGC TGGGACTAAG 
ATTCACGAAA CCGCCCCCGA CGAGCAGTCC ATTTGGAACG TCACAGTGCT CCCCAACAGT 
AAATGGGCCA ACATCACCTG GAAGCACAAT TTCAGGCCTG GAACTGACTT TGTGGTTGAG 
TACATCGACA GCAACCATAC GAAAAAAACT GTCCCTGTTA AGGCCCAGGC CCAGCCTATA 
CAGCTGACAG ACCTCTTTCC CGGGATGACG TACACGTTGC GGGTGTATTC CCGGGACAAC 
GAGGGCATCA GCAGTACCGT CATCACCTTT ATGACCAGTA CAGCTTACAC CAATAACCAG 
GCAGACATCG CCACCCAGGG CTGGTTCATC GGGCTCATGT GTGCCATTGC CCTTCTGGTG 
CTGATCCTTC TCATCGTCTG CTTCATCAAG AGGAGTCGAG GTGGCAAGTA CCCAGTGCGG 
GAAAAGAAGG ATGTCCCCTT GGGTCCTGAA GACCCCAAAG AAGAAGATGG CTCATTTGAC 
TACAGTGATG AGGACAACAA GCCCCTGCAG GGCAGCCAGA CATCTCTGGA TGGCACCATC 
AAGCAGCAGG AGAGCGATGA CAGCCTGGTG GACTATGGCG AAGGCGGCGA GGGCCAGTTC 
AATGAAGATG GCTCCTTTAT TGGCCAGTAC ACTGTCAAAA AGGACAAGGA GGAAACGGAG 
GGCAATGAGA GCTCAGAGGC CACATCACCA GTCAATGCCA TCTATTCCCT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248020.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246322.1 Mus musculus neurofascin (Nfasc), transcript variant X23, mRNA.

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CloneID	OMu74383
Clone ID Related Accession (Same CDS sequence)	XM_011248020.2 , XM_011248021.1 , XM_030254547.1 , XM_011248020.3
Accession Version	XM_011248020.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248020.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TACCCAAAGG GCGACCAGAC 
CGACCCAGGG ACCTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC 
CCAGGGGATG ACAACAACAG CCCTATCACA GACTACGTCG TTCAGTTTGA AGAGGACCAG 
TTCCAACCAG GGGTGTGGCA TGACCACTCC AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC 
CTCCATCTGT CCCCATATGT CAACTACCAA TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTTCC ATCCGAGCGG TACCGAACCA GTGGGGCACC CCCTGAATCT 
AATCCCAGTG ATGTGAAGGG CGAAGGGACA AGAAAGAACA ATATGGAGAT CACGTGGACG 
CCTATGAATG CTACCTCTGC CTTTGGCCCC AACCTACGCT ACATTGTCAA GTGGCGACGG 
AGAGAGACCC GAGAGACTTG GAACAATGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACGCCTG TCTACGTTCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTTGGGAAA 
GGCCCCGAGC CTGACACCAT CATTGGGTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGAGTC 
TACTCTGACA CGGTCCAGGG CCAGCTCAGA GAGTATCGAG CTTACTACTG GAGGGAAAGC 
AGTTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AGGCCAGCTT CCCTGGTGAC 
CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC CCCTACAGTA ACTACAAGCT GGAGATGGTG 
GTGGTCAATG GGAGAGGTGA CGGGCCTCGA AGTGAAACCA AGGAATTCAC CACCCCAGAA 
GGAGTACCCA GTGCCCCCAG GCGGTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC 
CTGGAGTGGG ACCACCCAGA GCACCCCAAC GGAATCCTGA TTGGATACAT CCTCAGATAC 
GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCT CTGTGCAGAG AGCAGACCCA GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC 
AGGACACAGG TGGGCTCTGG AGAAGCAGCC ACAGAGGAGT CCCCAGCACC TCCAAATGAA 
GCTACTCCAA CTGCAGCTCC TCCCACGTTG CCCCCGACTA CTGTGGGTAC CACAGGCCTT 
GTGAGCAGTA CTGATGCTAC TGCCCTTGCT GCCACCAGTG AAGCCACAAC AGTTCCCATC 
ATTCCAACCG TCGTACCTAC CACCGTCGCC ACCACCATTG CCACAACTAC TACAACCACT 
GCCGCCACCA CCACCACCAC CACTACGGAG AGCCCTCCCA CTACCACTGC TGGGACTAAG 
ATTCACGAAA CCGCCCCCGA CGAGCAGTCC ATTTGGAACG TCACAGTGCT CCCCAACAGT 
AAATGGGCCA ACATCACCTG GAAGCACAAT TTCAGGCCTG GAACTGACTT TGTGGTTGAG 
TACATCGACA GCAACCATAC GAAAAAAACT GTCCCTGTTA AGGCCCAGGC CCAGCCTATA 
CAGCTGACAG ACCTCTTTCC CGGGATGACG TACACGTTGC GGGTGTATTC CCGGGACAAC 
GAGGGCATCA GCAGTACCGT CATCACCTTT ATGACCAGTA CAGCTTACAC CAATAACCAG 
GCAGACATCG CCACCCAGGG CTGGTTCATC GGGCTCATGT GTGCCATTGC CCTTCTGGTG 
CTGATCCTTC TCATCGTCTG CTTCATCAAG AGGAGTCGAG GTGGCAAGTA CCCAGTGCGG 
GAAAAGAAGG ATGTCCCCTT GGGTCCTGAA GACCCCAAAG AAGAAGATGG CTCATTTGAC 
TACAGTGATG AGGACAACAA GCCCCTGCAG GGCAGCCAGA CATCTCTGGA TGGCACCATC 
AAGCAGCAGG AGAGCGATGA CAGCCTGGTG GACTATGGCG AAGGCGGCGA GGGCCAGTTC 
AATGAAGATG GCTCCTTTAT TGGCCAGTAC ACTGTCAAAA AGGACAAGGA GGAAACGGAG 
GGCAATGAGA GCTCAGAGGC CACATCACCA GTCAATGCCA TCTATTCCCT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246322.1
CDS	535..4611
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248020.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248020.3

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TACCCAAAGG GCGACCAGAC 
CGACCCAGGG ACCTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC 
CCAGGGGATG ACAACAACAG CCCTATCACA GACTACGTCG TTCAGTTTGA AGAGGACCAG 
TTCCAACCAG GGGTGTGGCA TGACCACTCC AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC 
CTCCATCTGT CCCCATATGT CAACTACCAA TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTTCC ATCCGAGCGG TACCGAACCA GTGGGGCACC CCCTGAATCT 
AATCCCAGTG ATGTGAAGGG CGAAGGGACA AGAAAGAACA ATATGGAGAT CACGTGGACG 
CCTATGAATG CTACCTCTGC CTTTGGCCCC AACCTACGCT ACATTGTCAA GTGGCGACGG 
AGAGAGACCC GAGAGACTTG GAACAATGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACGCCTG TCTACGTTCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTTGGGAAA 
GGCCCCGAGC CTGACACCAT CATTGGGTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGAGTC 
TACTCTGACA CGGTCCAGGG CCAGCTCAGA GAGTATCGAG CTTACTACTG GAGGGAAAGC 
AGTTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AGGCCAGCTT CCCTGGTGAC 
CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC CCCTACAGTA ACTACAAGCT GGAGATGGTG 
GTGGTCAATG GGAGAGGTGA CGGGCCTCGA AGTGAAACCA AGGAATTCAC CACCCCAGAA 
GGAGTACCCA GTGCCCCCAG GCGGTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC 
CTGGAGTGGG ACCACCCAGA GCACCCCAAC GGAATCCTGA TTGGATACAT CCTCAGATAC 
GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCT CTGTGCAGAG AGCAGACCCA GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC 
AGGACACAGG TGGGCTCTGG AGAAGCAGCC ACAGAGGAGT CCCCAGCACC TCCAAATGAA 
GCTACTCCAA CTGCAGCTCC TCCCACGTTG CCCCCGACTA CTGTGGGTAC CACAGGCCTT 
GTGAGCAGTA CTGATGCTAC TGCCCTTGCT GCCACCAGTG AAGCCACAAC AGTTCCCATC 
ATTCCAACCG TCGTACCTAC CACCGTCGCC ACCACCATTG CCACAACTAC TACAACCACT 
GCCGCCACCA CCACCACCAC CACTACGGAG AGCCCTCCCA CTACCACTGC TGGGACTAAG 
ATTCACGAAA CCGCCCCCGA CGAGCAGTCC ATTTGGAACG TCACAGTGCT CCCCAACAGT 
AAATGGGCCA ACATCACCTG GAAGCACAAT TTCAGGCCTG GAACTGACTT TGTGGTTGAG 
TACATCGACA GCAACCATAC GAAAAAAACT GTCCCTGTTA AGGCCCAGGC CCAGCCTATA 
CAGCTGACAG ACCTCTTTCC CGGGATGACG TACACGTTGC GGGTGTATTC CCGGGACAAC 
GAGGGCATCA GCAGTACCGT CATCACCTTT ATGACCAGTA CAGCTTACAC CAATAACCAG 
GCAGACATCG CCACCCAGGG CTGGTTCATC GGGCTCATGT GTGCCATTGC CCTTCTGGTG 
CTGATCCTTC TCATCGTCTG CTTCATCAAG AGGAGTCGAG GTGGCAAGTA CCCAGTGCGG 
GAAAAGAAGG ATGTCCCCTT GGGTCCTGAA GACCCCAAAG AAGAAGATGG CTCATTTGAC 
TACAGTGATG AGGACAACAA GCCCCTGCAG GGCAGCCAGA CATCTCTGGA TGGCACCATC 
AAGCAGCAGG AGAGCGATGA CAGCCTGGTG GACTATGGCG AAGGCGGCGA GGGCCAGTTC 
AATGAAGATG GCTCCTTTAT TGGCCAGTAC ACTGTCAAAA AGGACAAGGA GGAAACGGAG 
GGCAATGAGA GCTCAGAGGC CACATCACCA GTCAATGCCA TCTATTCCCT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_030254557.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110417.1 Mus musculus neurofascin (Nfasc), transcript variant X28, mRNA.

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CloneID	OMu74384
Clone ID Related Accession (Same CDS sequence)	XM_030254557.1
Accession Version	XM_030254557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCACC CGAGGAGTTG CAGAAAGAAC GCCCAGCTTC 
ATGTATCCCC AGGGCACATC GAGCAGTCAG ATGGTTCTCC GTGGCATGGA CCTGCTGCTG 
GAATGCATTG CCTCTGGCGT CCCAACACCA GACATTGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCATCTA ACAAGGCCAA GTTCGAGAAC TTTAATAAGG CTCTGCGCAT CACCAATGTC 
TCTGAAGAGG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCT CCATACTGGC TGGATGAGCC CAAGAACCTG 
ATCCTGGCTC CTGGGGAAGA TGGGAGGCTG GTATGCCGAG CCAATGGGAA CCCGAAGCCG 
ACCGTGCAGT GGATGGTGAA TGGAGAGCCT TTACAATCGG CACCACCCAA TCCCAACCGT 
GAGGTAGCTG GAGACACTAT CATCTTCCGG GATACTCAGA TCAGCAGCAG GGCAGTGTAC 
CAATGTAATA CATCCAATGA ACATGGCTAC CTGCTGGCCA ATGCCTTCGT CAGCGTGTTA 
GATGTACCCC CTCGGATGCT GTCTGCCCGC AACCAGCTCA TCAGGGTGAT CCTTTATAAC 
CGGACACGGC TGGACTGTCC GTTCTTTGGG TCTCCCATCC CAACACTCCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGCGGT AACTACCACG TCTACGAAAA CGGCAGTCTA 
GAAATCAAGA TGATTCGCAA AGAGGACCAA GGCATCTACA CCTGTGTGGC CACCAACATC 
CTGGGCAAAG CCGAAAATCA AGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACAGG 
ATGCCCGAGG ACCAGGTGGC CAAGAGGGGC ACCACGGTGC AGCTGGAGTG CCGCGTGAAA 
CATGACCCCT CCTTGAAGCT CACAGTCTCC TGGCTGAAGG ACGATGAGCC ACTCTACATT 
GGAAACAGGA TGAAGAAGGA AGATGACTCC CTGACGATCT TCGGAGTGGC AGAGCGGGAC 
CAGGGCAGTT ACACGTGTAT GGCCAGCACC GAGCTGGACC AGGACCTGGC AAAGGCCTAC 
CTCACTGTTC TAGCTGATCA GGCCACTCCA ACTAACCGTT TGGCTGCCCT ACCCAAAGGG 
CGACCAGACC GACCCAGGGA CCTGGAGCTC ACTGACCTGG CTGAGAGGAG TGTGAGGCTG 
ACCTGGATCC CAGGGGATGA CAACAACAGC CCTATCACAG ACTACGTCGT TCAGTTTGAA 
GAGGACCAGT TCCAACCAGG GGTGTGGCAT GACCACTCCA GGTTCCCAGG CAGCGTCAAC 
TCAGCCGTCC TCCATCTGTC CCCATATGTC AACTACCAAT TCAGAGTCAT CGCTGTCAAC 
GAGGTTGGGA GCAGCCACCC CAGCCTTCCA TCCGAGCGGT ACCGAACCAG TGGGGCACCC 
CCTGAATCTA ATCCCAGTGA TGTGAAGGGC GAAGGGACAA GAAAGAACAA TATGGAGATC 
ACGTGGACGC CTATGAATGC TACCTCTGCC TTTGGCCCCA ACCTACGCTA CATTGTCAAG 
TGGCGACGGA GAGAGACCCG AGAGACTTGG AACAATGTCA CAGTGTGGGG CTCTCGCTAC 
GTGGTGGGGC AGACGCCTGT CTACGTTCCC TATGAGATCC GAGTCCAGGC TGAAAATGAC 
TTTGGGAAAG GCCCCGAGCC TGACACCATC ATTGGGTACT CCGGAGAAGA TTATCCCAGG 
GCTGCGCCCA CTGAAGTTAA AATCCGAGTC CTGAACAGCA CAGCCATCAG CCTTCAGTGG 
AACCGAGTCT ACTCTGACAC GGTCCAGGGC CAGCTCAGAG AGTATCGAGC TTACTACTGG 
AGGGAAAGCA GTTTGCTGAA GAACCTGTGG GTGTCTCAGA AGAGACAGCA GGCCAGCTTC 
CCTGGTGACC GTCCCCGGGG CGTGGTGGCC CGCCTGTTCC CCTACAGTAA CTACAAGCTG 
GAGATGGTGG TGGTCAATGG GAGAGGTGAC GGGCCTCGAA GTGAAACCAA GGAATTCACC 
ACCCCAGAAG GAGTACCCAG TGCCCCCAGG CGGTTCAGAG TCCGACAGCC CAACCTGGAG 
ACCATCAACC TGGAGTGGGA CCACCCAGAG CACCCCAACG GAATCCTGAT TGGATACATC 
CTCAGATACG TGCCCTTTAA TGGAACCAAA CTGGGAAAGC AGATGGTGGA AAACTTCTCT 
CCCAATCAGA CCAAGTTCTC TGTGCAGAGA GCAGACCCAG TGTCGCGTTA CCGCTTCTCC 
CTCAGTGCCA GGACACAGGT GGGCTCTGGA GAAGCAGCCA CAGAGGAGTC CCCAGCACCT 
CCAAATGAAG CTACTCCAAC TGCAGCTCCT CCCACGTTGC CCCCGACTAC TGTGGGTACC 
ACAGGCCTTG TGAGCAGTAC TGATGCTACT GCCCTTGCTG CCACCAGTGA AGCCACAACA 
GTTCCCATCA TTCCAACCGT CGTACCTACC ACCGTCGCCA CCACCATTGC CACAACTACT 
ACAACCACTG CCGCCACCAC CACCACCACC ACTACGGAGA GCCCTCCCAC TACCACTGCT 
GGGACTAAGA TTCACGAAAC CGCCCCCGAC GAGCAGTCCA TTTGGAACGT CACAGTGCTC 
CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCAGGCCTGG AACTGACTTT 
GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAACTG TCCCTGTTAA GGCCCAGGCC 
CAGCCTATAC AGCTGACAGA CCTCTTTCCC GGGATGACGT ACACGTTGCG GGTGTATTCC 
CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC 
AATAACCAGG CAGACATCGC CACCCAGGGC TGGTTCATCG GGCTCATGTG TGCCATTGCC 
CTTCTGGTGC TGATCCTTCT CATCGTCTGC TTCATCAAGA GGAGTCGAGG TGGCAAGTAC 
CCAGTGCGGG AAAAGAAGGA TGTCCCCTTG GGTCCTGAAG ACCCCAAAGA AGAAGATGGC 
TCATTTGACT ACAGTGATGA GGACAACAAG CCCCTGCAGG GCAGCCAGAC ATCTCTGGAT 
GGCACCATCA AGCAGCAGGA GAGCGATGAC AGCCTGGTGG ACTATGGCGA AGGCGGCGAG 
GGCCAGTTCA ATGAAGATGG CTCCTTTATT GGCCAGTACA CTGTCAAAAA GGACAAGGAG 
GAAACGGAGG GCAATGAGAG CTCAGAGGCC ACATCACCAG TCAATGCCAT CTATTCCCTT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110417.1
CDS	196..4221
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_030254557.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254557.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCACC CGAGGAGTTG CAGAAAGAAC GCCCAGCTTC 
ATGTATCCCC AGGGCACATC GAGCAGTCAG ATGGTTCTCC GTGGCATGGA CCTGCTGCTG 
GAATGCATTG CCTCTGGCGT CCCAACACCA GACATTGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCATCTA ACAAGGCCAA GTTCGAGAAC TTTAATAAGG CTCTGCGCAT CACCAATGTC 
TCTGAAGAGG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCT CCATACTGGC TGGATGAGCC CAAGAACCTG 
ATCCTGGCTC CTGGGGAAGA TGGGAGGCTG GTATGCCGAG CCAATGGGAA CCCGAAGCCG 
ACCGTGCAGT GGATGGTGAA TGGAGAGCCT TTACAATCGG CACCACCCAA TCCCAACCGT 
GAGGTAGCTG GAGACACTAT CATCTTCCGG GATACTCAGA TCAGCAGCAG GGCAGTGTAC 
CAATGTAATA CATCCAATGA ACATGGCTAC CTGCTGGCCA ATGCCTTCGT CAGCGTGTTA 
GATGTACCCC CTCGGATGCT GTCTGCCCGC AACCAGCTCA TCAGGGTGAT CCTTTATAAC 
CGGACACGGC TGGACTGTCC GTTCTTTGGG TCTCCCATCC CAACACTCCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGCGGT AACTACCACG TCTACGAAAA CGGCAGTCTA 
GAAATCAAGA TGATTCGCAA AGAGGACCAA GGCATCTACA CCTGTGTGGC CACCAACATC 
CTGGGCAAAG CCGAAAATCA AGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACAGG 
ATGCCCGAGG ACCAGGTGGC CAAGAGGGGC ACCACGGTGC AGCTGGAGTG CCGCGTGAAA 
CATGACCCCT CCTTGAAGCT CACAGTCTCC TGGCTGAAGG ACGATGAGCC ACTCTACATT 
GGAAACAGGA TGAAGAAGGA AGATGACTCC CTGACGATCT TCGGAGTGGC AGAGCGGGAC 
CAGGGCAGTT ACACGTGTAT GGCCAGCACC GAGCTGGACC AGGACCTGGC AAAGGCCTAC 
CTCACTGTTC TAGCTGATCA GGCCACTCCA ACTAACCGTT TGGCTGCCCT ACCCAAAGGG 
CGACCAGACC GACCCAGGGA CCTGGAGCTC ACTGACCTGG CTGAGAGGAG TGTGAGGCTG 
ACCTGGATCC CAGGGGATGA CAACAACAGC CCTATCACAG ACTACGTCGT TCAGTTTGAA 
GAGGACCAGT TCCAACCAGG GGTGTGGCAT GACCACTCCA GGTTCCCAGG CAGCGTCAAC 
TCAGCCGTCC TCCATCTGTC CCCATATGTC AACTACCAAT TCAGAGTCAT CGCTGTCAAC 
GAGGTTGGGA GCAGCCACCC CAGCCTTCCA TCCGAGCGGT ACCGAACCAG TGGGGCACCC 
CCTGAATCTA ATCCCAGTGA TGTGAAGGGC GAAGGGACAA GAAAGAACAA TATGGAGATC 
ACGTGGACGC CTATGAATGC TACCTCTGCC TTTGGCCCCA ACCTACGCTA CATTGTCAAG 
TGGCGACGGA GAGAGACCCG AGAGACTTGG AACAATGTCA CAGTGTGGGG CTCTCGCTAC 
GTGGTGGGGC AGACGCCTGT CTACGTTCCC TATGAGATCC GAGTCCAGGC TGAAAATGAC 
TTTGGGAAAG GCCCCGAGCC TGACACCATC ATTGGGTACT CCGGAGAAGA TTATCCCAGG 
GCTGCGCCCA CTGAAGTTAA AATCCGAGTC CTGAACAGCA CAGCCATCAG CCTTCAGTGG 
AACCGAGTCT ACTCTGACAC GGTCCAGGGC CAGCTCAGAG AGTATCGAGC TTACTACTGG 
AGGGAAAGCA GTTTGCTGAA GAACCTGTGG GTGTCTCAGA AGAGACAGCA GGCCAGCTTC 
CCTGGTGACC GTCCCCGGGG CGTGGTGGCC CGCCTGTTCC CCTACAGTAA CTACAAGCTG 
GAGATGGTGG TGGTCAATGG GAGAGGTGAC GGGCCTCGAA GTGAAACCAA GGAATTCACC 
ACCCCAGAAG GAGTACCCAG TGCCCCCAGG CGGTTCAGAG TCCGACAGCC CAACCTGGAG 
ACCATCAACC TGGAGTGGGA CCACCCAGAG CACCCCAACG GAATCCTGAT TGGATACATC 
CTCAGATACG TGCCCTTTAA TGGAACCAAA CTGGGAAAGC AGATGGTGGA AAACTTCTCT 
CCCAATCAGA CCAAGTTCTC TGTGCAGAGA GCAGACCCAG TGTCGCGTTA CCGCTTCTCC 
CTCAGTGCCA GGACACAGGT GGGCTCTGGA GAAGCAGCCA CAGAGGAGTC CCCAGCACCT 
CCAAATGAAG CTACTCCAAC TGCAGCTCCT CCCACGTTGC CCCCGACTAC TGTGGGTACC 
ACAGGCCTTG TGAGCAGTAC TGATGCTACT GCCCTTGCTG CCACCAGTGA AGCCACAACA 
GTTCCCATCA TTCCAACCGT CGTACCTACC ACCGTCGCCA CCACCATTGC CACAACTACT 
ACAACCACTG CCGCCACCAC CACCACCACC ACTACGGAGA GCCCTCCCAC TACCACTGCT 
GGGACTAAGA TTCACGAAAC CGCCCCCGAC GAGCAGTCCA TTTGGAACGT CACAGTGCTC 
CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCAGGCCTGG AACTGACTTT 
GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAACTG TCCCTGTTAA GGCCCAGGCC 
CAGCCTATAC AGCTGACAGA CCTCTTTCCC GGGATGACGT ACACGTTGCG GGTGTATTCC 
CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC 
AATAACCAGG CAGACATCGC CACCCAGGGC TGGTTCATCG GGCTCATGTG TGCCATTGCC 
CTTCTGGTGC TGATCCTTCT CATCGTCTGC TTCATCAAGA GGAGTCGAGG TGGCAAGTAC 
CCAGTGCGGG AAAAGAAGGA TGTCCCCTTG GGTCCTGAAG ACCCCAAAGA AGAAGATGGC 
TCATTTGACT ACAGTGATGA GGACAACAAG CCCCTGCAGG GCAGCCAGAC ATCTCTGGAT 
GGCACCATCA AGCAGCAGGA GAGCGATGAC AGCCTGGTGG ACTATGGCGA AGGCGGCGAG 
GGCCAGTTCA ATGAAGATGG CTCCTTTATT GGCCAGTACA CTGTCAAAAA GGACAAGGAG 
GAAACGGAGG GCAATGAGAG CTCAGAGGCC ACATCACCAG TCAATGCCAT CTATTCCCTT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_030254565.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110425.1 Mus musculus neurofascin (Nfasc), transcript variant X35, mRNA.

Price & Availability↑

CloneID	OMu74385
Clone ID Related Accession (Same CDS sequence)	XM_030254565.1
Accession Version	XM_030254565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTTTT GGTTCCTATC ATTAGTCTCT CCTCATCACT TCCCCACCCC TTTCCAGGTG 
CTCTGTCCTG AGTCTCTATC TCCTCCCCGA CAGAGCTTGT ATTCCCAGCT CAGAAGCTGG 
GGCCTGCTGC TGCAGCCAGT GGTGAGAGCC CTTGGCTGGG GACTGGGAAC TGCACCCTTG 
CCACAAGACA GCTATGATTG GTTCCCTGCC TGGGAGTGGC TCTCTGGTTT GGAGGTATAT 
TTGCAGGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110425.1
CDS	53..3124
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDFWFLSLVSPHHFPTPFQVLCPESLSPPRQSLYSQLRSWGLLLQPVVRALGWGLGTAPLPQDSYDWFPAWEWLSGLEVYLQD

Target ORF information:

RefSeq Version	XM_030254565.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254565.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTTTT GGTTCCTATC ATTAGTCTCT CCTCATCACT TCCCCACCCC TTTCCAGGTG 
CTCTGTCCTG AGTCTCTATC TCCTCCCCGA CAGAGCTTGT ATTCCCAGCT CAGAAGCTGG 
GGCCTGCTGC TGCAGCCAGT GGTGAGAGCC CTTGGCTGGG GACTGGGAAC TGCACCCTTG 
CCACAAGACA GCTATGATTG GTTCCCTGCC TGGGAGTGGC TCTCTGGTTT GGAGGTATAT 
TTGCAGGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248025.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246327.1 Mus musculus neurofascin (Nfasc), transcript variant X36, mRNA.

Price & Availability↑

CloneID	OMu74387
Clone ID Related Accession (Same CDS sequence)	XM_011248025.3 , XM_011248025.2
Accession Version	XM_011248025.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248025.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAG TTAGACATAA AAGAGAGATA TCACAACAGA TTTCAATGAA GTCGAGAGAA 
TATGTAGGAC ATAGTTTAGT AAAACATGAT TTGGAAAGCC TAGAAGAAAC AGCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC GTTGCCCCCG 
ACTACTGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC 
AGTGAAGCCA CAACAGTTCC CATCATTCCA ACCGTCGTAC CTACCACCGT CGCCACCACC 
ATTGCCACAA CTACTACAAC CACTGCCGCC ACCACCACCA CCACCACTAC GGAGAGCCCT 
CCCACTACCA CTGCTGGGAC TAAGATTCAC GAAACCGCCC CCGACGAGCA GTCCATTTGG 
AACGTCACAG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCAGG 
CCTGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AGGCCCAGCC TATACAGCTG ACAGACCTCT TTCCCGGGAT GACGTACACG 
TTGCGGGTGT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC 
AGTACAGCTT ACACCAATAA CCAGGCAGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC 
ATGTGTGCCA TTGCCCTTCT GGTGCTGATC CTTCTCATCG TCTGCTTCAT CAAGAGGAGT 
CGAGGTGGCA AGTACCCAGT GCGGGAAAAG AAGGATGTCC CCTTGGGTCC TGAAGACCCC 
AAAGAAGAAG ATGGCTCATT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACATCTC TGGATGGCAC CATCAAGCAG CAGGAGAGCG ATGACAGCCT GGTGGACTAT 
GGCGAAGGCG GCGAGGGCCA GTTCAATGAA GATGGCTCCT TTATTGGCCA GTACACTGTC 
AAAAAGGACA AGGAGGAAAC GGAGGGCAAT GAGAGCTCAG AGGCCACATC ACCAGTCAAT 
GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246327.1
CDS	5456..8476
Translation	MNKVRHKREISQQISMKSREYVGHSLVKHDLESLEETAAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248025.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248025.3

ORF Insert Sequence:

ATGAATAAAG TTAGACATAA AAGAGAGATA TCACAACAGA TTTCAATGAA GTCGAGAGAA 
TATGTAGGAC ATAGTTTAGT AAAACATGAT TTGGAAAGCC TAGAAGAAAC AGCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC GTTGCCCCCG 
ACTACTGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC 
AGTGAAGCCA CAACAGTTCC CATCATTCCA ACCGTCGTAC CTACCACCGT CGCCACCACC 
ATTGCCACAA CTACTACAAC CACTGCCGCC ACCACCACCA CCACCACTAC GGAGAGCCCT 
CCCACTACCA CTGCTGGGAC TAAGATTCAC GAAACCGCCC CCGACGAGCA GTCCATTTGG 
AACGTCACAG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCAGG 
CCTGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AGGCCCAGCC TATACAGCTG ACAGACCTCT TTCCCGGGAT GACGTACACG 
TTGCGGGTGT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC 
AGTACAGCTT ACACCAATAA CCAGGCAGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC 
ATGTGTGCCA TTGCCCTTCT GGTGCTGATC CTTCTCATCG TCTGCTTCAT CAAGAGGAGT 
CGAGGTGGCA AGTACCCAGT GCGGGAAAAG AAGGATGTCC CCTTGGGTCC TGAAGACCCC 
AAAGAAGAAG ATGGCTCATT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACATCTC TGGATGGCAC CATCAAGCAG CAGGAGAGCG ATGACAGCCT GGTGGACTAT 
GGCGAAGGCG GCGAGGGCCA GTTCAATGAA GATGGCTCCT TTATTGGCCA GTACACTGTC 
AAAAAGGACA AGGAGGAAAC GGAGGGCAAT GAGAGCTCAG AGGCCACATC ACCAGTCAAT 
GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017320885.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176374.1 Mus musculus neurofascin (Nfasc), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OMu77139
Clone ID Related Accession (Same CDS sequence)	XM_017320885.1
Accession Version	XM_017320885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGGG CGACCAGACC GACCCAGGGA CCTGGAGCTC 
ACTGACCTGG CTGAGAGGAG TGTGAGGCTG ACCTGGATCC CAGGGGATGA CAACAACAGC 
CCTATCACAG ACTACGTCGT TCAGTTTGAA GAGGACCAGT TCCAACCAGG GGTGTGGCAT 
GACCACTCCA GGTTCCCAGG CAGCGTCAAC TCAGCCGTCC TCCATCTGTC CCCATATGTC 
AACTACCAAT TCAGAGTCAT CGCTGTCAAC GAGGTTGGGA GCAGCCACCC CAGCCTTCCA 
TCCGAGCGGT ACCGAACCAG TGGGGCACCC CCTGAATCTA ATCCCAGTGA TGTGAAGGGC 
GAAGGGACAA GAAAGAACAA TATGGAGATC ACGTGGACGC CTATGAATGC TACCTCTGCC 
TTTGGCCCCA ACCTACGCTA CATTGTCAAG TGGCGACGGA GAGAGACCCG AGAGACTTGG 
AACAATGTCA CAGTGTGGGG CTCTCGCTAC GTGGTGGGGC AGACGCCTGT CTACGTTCCC 
TATGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAAG GCCCCGAGCC TGACACCATC 
ATTGGGTACT CCGGAGAAGA TTATCCCAGG GCTGCGCCCA CTGAAGTTAA AATCCGAGTC 
CTGAACAGCA CAGCCATCAG CCTTCAGTGG AACCGAGTCT ACTCTGACAC GGTCCAGGGC 
CAGCTCAGAG AGTATCGAGC TTACTACTGG AGGGAAAGCA GTTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGACAGCA GGCCAGCTTC CCTGGTGACC GTCCCCGGGG CGTGGTGGCC 
CGCCTGTTCC CCTACAGTAA CTACAAGCTG GAGATGGTGG TGGTCAATGG GAGAGGTGAC 
GGGCCTCGAA GTGAAACCAA GGAATTCACC ACCCCAGAAG GAGTACCCAG TGCCCCCAGG 
CGGTTCAGAG TCCGACAGCC CAACCTGGAG ACCATCAACC TGGAGTGGGA CCACCCAGAG 
CACCCCAACG GAATCCTGAT TGGATACATC CTCAGATACG TGCCCTTTAA TGGAACCAAA 
CTGGGAAAGC AGATGGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCTC TGTGCAGAGA 
GCAGACCCAG TGTCGCGTTA CCGCTTCTCC CTCAGTGCCA GGACACAGGT GGGCTCTGGA 
GAAGCAGCCA CAGAGGAGTC CCCAGCACCT CCAAATGAAG CTACTCCAAC TGCAGCTTAC 
ACCAATAACC AGGCAGACAT CGCCACCCAG GGCTGGTTCA TCGGGCTCAT GTGTGCCATT 
GCCCTTCTGG TGCTGATCCT TCTCATCGTC TGCTTCATCA AGAGGAGTCG AGGTGGCAAG 
TACCCAGTGC GGGAAAAGAA GGATGTCCCC TTGGGTCCTG AAGACCCCAA AGAAGAAGAT 
GGCTCATTTG ACTACAGTGA TGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACATCTCTG 
GATGGCACCA TCAAGCAGCA GGAGAGCGAT GACAGCCTGG TGGACTATGG CGAAGGCGGC 
GAGGGCCAGT TCAATGAAGA TGGCTCCTTT ATTGGCCAGT ACACTGTCAA AAAGGACAAG 
GAGGAAACGG AGGGCAATGA GAGCTCAGAG GCCACATCAC CAGTCAATGC CATCTATTCC 
CTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176374.1
CDS	55..3903
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017320885.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320885.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGGG CGACCAGACC GACCCAGGGA CCTGGAGCTC 
ACTGACCTGG CTGAGAGGAG TGTGAGGCTG ACCTGGATCC CAGGGGATGA CAACAACAGC 
CCTATCACAG ACTACGTCGT TCAGTTTGAA GAGGACCAGT TCCAACCAGG GGTGTGGCAT 
GACCACTCCA GGTTCCCAGG CAGCGTCAAC TCAGCCGTCC TCCATCTGTC CCCATATGTC 
AACTACCAAT TCAGAGTCAT CGCTGTCAAC GAGGTTGGGA GCAGCCACCC CAGCCTTCCA 
TCCGAGCGGT ACCGAACCAG TGGGGCACCC CCTGAATCTA ATCCCAGTGA TGTGAAGGGC 
GAAGGGACAA GAAAGAACAA TATGGAGATC ACGTGGACGC CTATGAATGC TACCTCTGCC 
TTTGGCCCCA ACCTACGCTA CATTGTCAAG TGGCGACGGA GAGAGACCCG AGAGACTTGG 
AACAATGTCA CAGTGTGGGG CTCTCGCTAC GTGGTGGGGC AGACGCCTGT CTACGTTCCC 
TATGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAAG GCCCCGAGCC TGACACCATC 
ATTGGGTACT CCGGAGAAGA TTATCCCAGG GCTGCGCCCA CTGAAGTTAA AATCCGAGTC 
CTGAACAGCA CAGCCATCAG CCTTCAGTGG AACCGAGTCT ACTCTGACAC GGTCCAGGGC 
CAGCTCAGAG AGTATCGAGC TTACTACTGG AGGGAAAGCA GTTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGACAGCA GGCCAGCTTC CCTGGTGACC GTCCCCGGGG CGTGGTGGCC 
CGCCTGTTCC CCTACAGTAA CTACAAGCTG GAGATGGTGG TGGTCAATGG GAGAGGTGAC 
GGGCCTCGAA GTGAAACCAA GGAATTCACC ACCCCAGAAG GAGTACCCAG TGCCCCCAGG 
CGGTTCAGAG TCCGACAGCC CAACCTGGAG ACCATCAACC TGGAGTGGGA CCACCCAGAG 
CACCCCAACG GAATCCTGAT TGGATACATC CTCAGATACG TGCCCTTTAA TGGAACCAAA 
CTGGGAAAGC AGATGGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCTC TGTGCAGAGA 
GCAGACCCAG TGTCGCGTTA CCGCTTCTCC CTCAGTGCCA GGACACAGGT GGGCTCTGGA 
GAAGCAGCCA CAGAGGAGTC CCCAGCACCT CCAAATGAAG CTACTCCAAC TGCAGCTTAC 
ACCAATAACC AGGCAGACAT CGCCACCCAG GGCTGGTTCA TCGGGCTCAT GTGTGCCATT 
GCCCTTCTGG TGCTGATCCT TCTCATCGTC TGCTTCATCA AGAGGAGTCG AGGTGGCAAG 
TACCCAGTGC GGGAAAAGAA GGATGTCCCC TTGGGTCCTG AAGACCCCAA AGAAGAAGAT 
GGCTCATTTG ACTACAGTGA TGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACATCTCTG 
GATGGCACCA TCAAGCAGCA GGAGAGCGAT GACAGCCTGG TGGACTATGG CGAAGGCGGC 
GAGGGCCAGT TCAATGAAGA TGGCTCCTTT ATTGGCCAGT ACACTGTCAA AAAGGACAAG 
GAGGAAACGG AGGGCAATGA GAGCTCAGAG GCCACATCAC CAGTCAATGC CATCTATTCC 
CTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017320887.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176376.1 Mus musculus neurofascin (Nfasc), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu77140
Clone ID Related Accession (Same CDS sequence)	XM_017320887.1
Accession Version	XM_017320887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTTAC CCAGTGCCCC CAGGCGGTTC AGAGTCCGAC AGCCCAACCT GGAGACCATC 
AACCTGGAGT GGGACCACCC AGAGCACCCC AACGGAATCC TGATTGGATA CATCCTCAGA 
TACGTGCCCT TTAATGGAAC CAAACTGGGA AAGCAGATGG TGGAAAACTT CTCTCCCAAT 
CAGACCAAGT TCTCTGTGCA GAGAGCAGAC CCAGTGTCGC GTTACCGCTT CTCCCTCAGT 
GCCAGGACAC AGGTGGGCTC TGGAGAAGCA GCCACAGAGG AGTCCCCAGC ACCTCCAAAT 
GAAGCTACTC CAACTGCAGC CCCCGACGAG CAGTCCATTT GGAACGTCAC AGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCA GGCCTGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCTATACAGC TGACAGACCT CTTTCCCGGG ATGACGTACA CGTTGCGGGT GTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT 
AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT 
CTGGTGCTGA TCCTTCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAGTACCCA 
GTGCGGGAAA AGAAGGATGT CCCCTTGGGT CCTGAAGACC CCAAAGAAGA AGATGGCTCA 
TTTGACTACA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGATGGC 
ACCATCAAGC AGCAGGAGAG CGATGACAGC CTGGTGGACT ATGGCGAAGG CGGCGAGGGC 
CAGTTCAATG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAAAAAGGA CAAGGAGGAA 
ACGGAGGGCA ATGAGAGCTC AGAGGCCACA TCACCAGTCA ATGCCATCTA TTCCCTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176376.1
CDS	54..3896
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017320887.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320887.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTTAC CCAGTGCCCC CAGGCGGTTC AGAGTCCGAC AGCCCAACCT GGAGACCATC 
AACCTGGAGT GGGACCACCC AGAGCACCCC AACGGAATCC TGATTGGATA CATCCTCAGA 
TACGTGCCCT TTAATGGAAC CAAACTGGGA AAGCAGATGG TGGAAAACTT CTCTCCCAAT 
CAGACCAAGT TCTCTGTGCA GAGAGCAGAC CCAGTGTCGC GTTACCGCTT CTCCCTCAGT 
GCCAGGACAC AGGTGGGCTC TGGAGAAGCA GCCACAGAGG AGTCCCCAGC ACCTCCAAAT 
GAAGCTACTC CAACTGCAGC CCCCGACGAG CAGTCCATTT GGAACGTCAC AGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCA GGCCTGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCTATACAGC TGACAGACCT CTTTCCCGGG ATGACGTACA CGTTGCGGGT GTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT 
AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT 
CTGGTGCTGA TCCTTCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAGTACCCA 
GTGCGGGAAA AGAAGGATGT CCCCTTGGGT CCTGAAGACC CCAAAGAAGA AGATGGCTCA 
TTTGACTACA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGATGGC 
ACCATCAAGC AGCAGGAGAG CGATGACAGC CTGGTGGACT ATGGCGAAGG CGGCGAGGGC 
CAGTTCAATG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAAAAAGGA CAAGGAGGAA 
ACGGAGGGCA ATGAGAGCTC AGAGGCCACA TCACCAGTCA ATGCCATCTA TTCCCTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017320894.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176383.1 Mus musculus neurofascin (Nfasc), transcript variant X19, mRNA.

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CloneID	OMu77141
Clone ID Related Accession (Same CDS sequence)	XM_017320894.1
Accession Version	XM_017320894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTTAC CCAGTGCCCC CAGGCGGTTC AGAGTCCGAC AGCCCAACCT GGAGACCATC 
AACCTGGAGT GGGACCACCC AGAGCACCCC AACGGAATCC TGATTGGATA CATCCTCAGA 
TACGTGCCCT TTAATGGAAC CAAACTGGGA AAGCAGATGG TGGAAAACTT CTCTCCCAAT 
CAGACCAAGT TCTCTGTGCA GAGAGCAGAC CCAGTGTCGC GTTACCGCTT CTCCCTCAGT 
GCCAGGACAC AGGTGGGCTC TGGAGAAGCA GCCACAGAGG AGTCCCCAGC ACCTCCAAAT 
GAAGCTACTC CAACTGCAGC TCCTCCCACG TTGCCCCCGA CTACTGTGGG TACCACAGGC 
CTTGTGAGCA GTACTGATGC TACTGCCCTT GCTGCCACCA GTGAAGCCAC AACAGTTCCC 
ATCATTCCAA CCGTCGTACC TACCACCGTC GCCACCACCA TTGCCACAAC TACTACAACC 
ACTGCCGCCA CCACCACCAC CACCACTACG GAGAGCCCTC CCACTACCAC TGCTGGGACT 
AAGATTCACG AAACCGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TTCTCATCGT CTGCTTCATC 
AAGAGGAGTC GAGGTGGCAA GTACCCAGTG CGGGAAAAGA AGGATGTCCC CTTGGGTCCT 
GAAGACCCCA AAGAAGAAGA TGGCTCATTT GACTACAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGATGGCACC ATCAAGCAGC AGGAGAGCGA TGACAGCCTG 
GTGGACTATG GCGAAGGCGG CGAGGGCCAG TTCAATGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA AAAAGGACAA GGAGGAAACG GAGGGCAATG AGAGCTCAGA GGCCACATCA 
CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176383.1
CDS	53..3862
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017320894.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320894.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTTAC CCAGTGCCCC CAGGCGGTTC AGAGTCCGAC AGCCCAACCT GGAGACCATC 
AACCTGGAGT GGGACCACCC AGAGCACCCC AACGGAATCC TGATTGGATA CATCCTCAGA 
TACGTGCCCT TTAATGGAAC CAAACTGGGA AAGCAGATGG TGGAAAACTT CTCTCCCAAT 
CAGACCAAGT TCTCTGTGCA GAGAGCAGAC CCAGTGTCGC GTTACCGCTT CTCCCTCAGT 
GCCAGGACAC AGGTGGGCTC TGGAGAAGCA GCCACAGAGG AGTCCCCAGC ACCTCCAAAT 
GAAGCTACTC CAACTGCAGC TCCTCCCACG TTGCCCCCGA CTACTGTGGG TACCACAGGC 
CTTGTGAGCA GTACTGATGC TACTGCCCTT GCTGCCACCA GTGAAGCCAC AACAGTTCCC 
ATCATTCCAA CCGTCGTACC TACCACCGTC GCCACCACCA TTGCCACAAC TACTACAACC 
ACTGCCGCCA CCACCACCAC CACCACTACG GAGAGCCCTC CCACTACCAC TGCTGGGACT 
AAGATTCACG AAACCGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TTCTCATCGT CTGCTTCATC 
AAGAGGAGTC GAGGTGGCAA GTACCCAGTG CGGGAAAAGA AGGATGTCCC CTTGGGTCCT 
GAAGACCCCA AAGAAGAAGA TGGCTCATTT GACTACAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGATGGCACC ATCAAGCAGC AGGAGAGCGA TGACAGCCTG 
GTGGACTATG GCGAAGGCGG CGAGGGCCAG TTCAATGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA AAAAGGACAA GGAGGAAACG GAGGGCAATG AGAGCTCAGA GGCCACATCA 
CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017320897.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176386.1 Mus musculus neurofascin (Nfasc), transcript variant X24, mRNA.

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CloneID	OMu77142
Clone ID Related Accession (Same CDS sequence)	XM_017320897.1
Accession Version	XM_017320897.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTTAC CCAGTGCCCC CAGGCGGTTC AGAGTCCGAC AGCCCAACCT GGAGACCATC 
AACCTGGAGT GGGACCACCC AGAGCACCCC AACGGAATCC TGATTGGATA CATCCTCAGA 
TACGTGCCCT TTAATGGAAC CAAACTGGGA AAGCAGATGG TGGAAAACTT CTCTCCCAAT 
CAGACCAAGT TCTCTGTGCA GAGAGCAGAC CCAGTGTCGC GTTACCGCTT CTCCCTCAGT 
GCCAGGACAC AGGTGGGCTC TGGAGAAGCA GCCACAGAGG AGTCCCCAGC ACCTCCAAAT 
GAAGCTACTC CAACTGCAGC TTACACCAAT AACCAGGCAG ACATCGCCAC CCAGGGCTGG 
TTCATCGGGC TCATGTGTGC CATTGCCCTT CTGGTGCTGA TCCTTCTCAT CGTCTGCTTC 
ATCAAGAGGA GTCGAGGTGG CAAGTACCCA GTGCGGGAAA AGAAGGATGT CCCCTTGGGT 
CCTGAAGACC CCAAAGAAGA AGATGGCTCA TTTGACTACA GTGATGAGGA CAACAAGCCC 
CTGCAGGGCA GCCAGACATC TCTGGATGGC ACCATCAAGC AGCAGGAGAG CGATGACAGC 
CTGGTGGACT ATGGCGAAGG CGGCGAGGGC CAGTTCAATG AAGATGGCTC CTTTATTGGC 
CAGTACACTG TCAAAAAGGA CAAGGAGGAA ACGGAGGGCA ATGAGAGCTC AGAGGCCACA 
TCACCAGTCA ATGCCATCTA TTCCCTTGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176386.1
CDS	53..3625
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017320897.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320897.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTTAC CCAGTGCCCC CAGGCGGTTC AGAGTCCGAC AGCCCAACCT GGAGACCATC 
AACCTGGAGT GGGACCACCC AGAGCACCCC AACGGAATCC TGATTGGATA CATCCTCAGA 
TACGTGCCCT TTAATGGAAC CAAACTGGGA AAGCAGATGG TGGAAAACTT CTCTCCCAAT 
CAGACCAAGT TCTCTGTGCA GAGAGCAGAC CCAGTGTCGC GTTACCGCTT CTCCCTCAGT 
GCCAGGACAC AGGTGGGCTC TGGAGAAGCA GCCACAGAGG AGTCCCCAGC ACCTCCAAAT 
GAAGCTACTC CAACTGCAGC TTACACCAAT AACCAGGCAG ACATCGCCAC CCAGGGCTGG 
TTCATCGGGC TCATGTGTGC CATTGCCCTT CTGGTGCTGA TCCTTCTCAT CGTCTGCTTC 
ATCAAGAGGA GTCGAGGTGG CAAGTACCCA GTGCGGGAAA AGAAGGATGT CCCCTTGGGT 
CCTGAAGACC CCAAAGAAGA AGATGGCTCA TTTGACTACA GTGATGAGGA CAACAAGCCC 
CTGCAGGGCA GCCAGACATC TCTGGATGGC ACCATCAAGC AGCAGGAGAG CGATGACAGC 
CTGGTGGACT ATGGCGAAGG CGGCGAGGGC CAGTTCAATG AAGATGGCTC CTTTATTGGC 
CAGTACACTG TCAAAAAGGA CAAGGAGGAA ACGGAGGGCA ATGAGAGCTC AGAGGCCACA 
TCACCAGTCA ATGCCATCTA TTCCCTTGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017320900.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176389.1 Mus musculus neurofascin (Nfasc), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OMu77143
Clone ID Related Accession (Same CDS sequence)	XM_017320900.1
Accession Version	XM_017320900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTTAC CCAGTGCCCC CAGGCGGTTC AGAGTCCGAC AGCCCAACCT GGAGACCATC 
AACCTGGAGT GGGACCACCC AGAGCACCCC AACGGAATCC TGATTGGATA CATCCTCAGA 
TACGTGCCCT TTAATGGAAC CAAACTGGGA AAGCAGATGG TGGAAAACTT CTCTCCCAAT 
CAGACCAAGT TCTCTGTGCA GAGAGCAGAC CCAGTGTCGC GTTACCGCTT CTCCCTCAGT 
GCCAGGACAC AGGTGGGCTC TGGAGAAGCA GCCACAGAGG AGTCCCCAGC ACCTCCAAAT 
GAAGCTTACA CCAATAACCA GGCAGACATC GCCACCCAGG GCTGGTTCAT CGGGCTCATG 
TGTGCCATTG CCCTTCTGGT GCTGATCCTT CTCATCGTCT GCTTCATCAA GAGGAGTCGA 
GGTGGCAAGT ACCCAGTGCG GGAAAAGAAG GATGTCCCCT TGGGTCCTGA AGACCCCAAA 
GAAGAAGATG GCTCATTTGA CTACAGTGAT GAGGACAACA AGCCCCTGCA GGGCAGCCAG 
ACATCTCTGG ATGGCACCAT CAAGCAGCAG GAGAGCGATG ACAGCCTGGT GGACTATGGC 
GAAGGCGGCG AGGGCCAGTT CAATGAAGAT GGCTCCTTTA TTGGCCAGTA CACTGTCAAA 
AAGGACAAGG AGGAAACGGA GGGCAATGAG AGCTCAGAGG CCACATCACC AGTCAATGCC 
ATCTATTCCC TTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176389.1
CDS	53..3610
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017320900.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320900.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTTAC CCAGTGCCCC CAGGCGGTTC AGAGTCCGAC AGCCCAACCT GGAGACCATC 
AACCTGGAGT GGGACCACCC AGAGCACCCC AACGGAATCC TGATTGGATA CATCCTCAGA 
TACGTGCCCT TTAATGGAAC CAAACTGGGA AAGCAGATGG TGGAAAACTT CTCTCCCAAT 
CAGACCAAGT TCTCTGTGCA GAGAGCAGAC CCAGTGTCGC GTTACCGCTT CTCCCTCAGT 
GCCAGGACAC AGGTGGGCTC TGGAGAAGCA GCCACAGAGG AGTCCCCAGC ACCTCCAAAT 
GAAGCTTACA CCAATAACCA GGCAGACATC GCCACCCAGG GCTGGTTCAT CGGGCTCATG 
TGTGCCATTG CCCTTCTGGT GCTGATCCTT CTCATCGTCT GCTTCATCAA GAGGAGTCGA 
GGTGGCAAGT ACCCAGTGCG GGAAAAGAAG GATGTCCCCT TGGGTCCTGA AGACCCCAAA 
GAAGAAGATG GCTCATTTGA CTACAGTGAT GAGGACAACA AGCCCCTGCA GGGCAGCCAG 
ACATCTCTGG ATGGCACCAT CAAGCAGCAG GAGAGCGATG ACAGCCTGGT GGACTATGGC 
GAAGGCGGCG AGGGCCAGTT CAATGAAGAT GGCTCCTTTA TTGGCCAGTA CACTGTCAAA 
AAGGACAAGG AGGAAACGGA GGGCAATGAG AGCTCAGAGG CCACATCACC AGTCAATGCC 
ATCTATTCCC TTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017320902.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176391.1 Mus musculus neurofascin (Nfasc), transcript variant X30, mRNA.

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CloneID	OMu77144
Clone ID Related Accession (Same CDS sequence)	XM_017320902.1
Accession Version	XM_017320902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGGG CGACCAGACC GACCCAGGGA CCTGGAGCTC 
ACTGACCTGG CTGAGAGGAG TGTGAGGCTG ACCTGGATCC CAGGGGATGA CAACAACAGC 
CCTATCACAG ACTACGTCGT TCAGTTTGAA GAGGACCAGT TCCAACCAGG GGTGTGGCAT 
GACCACTCCA GGTTCCCAGG CAGCGTCAAC TCAGCCGTCC TCCATCTGTC CCCATATGTC 
AACTACCAAT TCAGAGTCAT CGCTGTCAAC GAGGTTGGGA GCAGCCACCC CAGCCTTCCA 
TCCGAGCGGT ACCGAACCAG TGGGGCACCC CCTGAATCTA ATCCCAGTGA TGTGAAGGGC 
GAAGGGACAA GAAAGAACAA TATGGAGATC ACGTGGACGC CTATGAATGC TACCTCTGCC 
TTTGGCCCCA ACCTACGCTA CATTGTCAAG TGGCGACGGA GAGAGACCCG AGAGACTTGG 
AACAATGTCA CAGTGTGGGG CTCTCGCTAC GTGGTGGGGC AGACGCCTGT CTACGTTCCC 
TATGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAAG GCCCCGAGCC TGACACCATC 
ATTGGGTACT CCGGAGAAGA TTTACCCAGT GCCCCCAGGC GGTTCAGAGT CCGACAGCCC 
AACCTGGAGA CCATCAACCT GGAGTGGGAC CACCCAGAGC ACCCCAACGG AATCCTGATT 
GGATACATCC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCTCT GTGCAGAGAG CAGACCCAGT GTCGCGTTAC 
CGCTTCTCCC TCAGTGCCAG GACACAGGTG GGCTCTGGAG AAGCAGCCAC AGAGGAGTCC 
CCAGCACCTC CAAATGAAGC TTACACCAAT AACCAGGCAG ACATCGCCAC CCAGGGCTGG 
TTCATCGGGC TCATGTGTGC CATTGCCCTT CTGGTGCTGA TCCTTCTCAT CGTCTGCTTC 
ATCAAGAGGA GTCGAGGTGG CAAGTACCCA GTGCGGGAAA AGAAGGATGT CCCCTTGGGT 
CCTGAAGACC CCAAAGAAGA AGATGGCTCA TTTGACTACA GTGATGAGGA CAACAAGCCC 
CTGCAGGGCA GCCAGACATC TCTGGATGGC ACCATCAAGC AGCAGGAGAG CGATGACAGC 
CTGGTGGACT ATGGCGAAGG CGGCGAGGGC CAGTTCAATG AAGATGGCTC CTTTATTGGC 
CAGTACACTG TCAAAAAGGA CAAGGAGGAA ACGGAGGGCA ATGAGAGCTC AGAGGCCACA 
TCACCAGTCA ATGCCATCTA TTCCCTTGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176391.1
CDS	53..3565
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017320902.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320902.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGGG CGACCAGACC GACCCAGGGA CCTGGAGCTC 
ACTGACCTGG CTGAGAGGAG TGTGAGGCTG ACCTGGATCC CAGGGGATGA CAACAACAGC 
CCTATCACAG ACTACGTCGT TCAGTTTGAA GAGGACCAGT TCCAACCAGG GGTGTGGCAT 
GACCACTCCA GGTTCCCAGG CAGCGTCAAC TCAGCCGTCC TCCATCTGTC CCCATATGTC 
AACTACCAAT TCAGAGTCAT CGCTGTCAAC GAGGTTGGGA GCAGCCACCC CAGCCTTCCA 
TCCGAGCGGT ACCGAACCAG TGGGGCACCC CCTGAATCTA ATCCCAGTGA TGTGAAGGGC 
GAAGGGACAA GAAAGAACAA TATGGAGATC ACGTGGACGC CTATGAATGC TACCTCTGCC 
TTTGGCCCCA ACCTACGCTA CATTGTCAAG TGGCGACGGA GAGAGACCCG AGAGACTTGG 
AACAATGTCA CAGTGTGGGG CTCTCGCTAC GTGGTGGGGC AGACGCCTGT CTACGTTCCC 
TATGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAAG GCCCCGAGCC TGACACCATC 
ATTGGGTACT CCGGAGAAGA TTTACCCAGT GCCCCCAGGC GGTTCAGAGT CCGACAGCCC 
AACCTGGAGA CCATCAACCT GGAGTGGGAC CACCCAGAGC ACCCCAACGG AATCCTGATT 
GGATACATCC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCTCT GTGCAGAGAG CAGACCCAGT GTCGCGTTAC 
CGCTTCTCCC TCAGTGCCAG GACACAGGTG GGCTCTGGAG AAGCAGCCAC AGAGGAGTCC 
CCAGCACCTC CAAATGAAGC TTACACCAAT AACCAGGCAG ACATCGCCAC CCAGGGCTGG 
TTCATCGGGC TCATGTGTGC CATTGCCCTT CTGGTGCTGA TCCTTCTCAT CGTCTGCTTC 
ATCAAGAGGA GTCGAGGTGG CAAGTACCCA GTGCGGGAAA AGAAGGATGT CCCCTTGGGT 
CCTGAAGACC CCAAAGAAGA AGATGGCTCA TTTGACTACA GTGATGAGGA CAACAAGCCC 
CTGCAGGGCA GCCAGACATC TCTGGATGGC ACCATCAAGC AGCAGGAGAG CGATGACAGC 
CTGGTGGACT ATGGCGAAGG CGGCGAGGGC CAGTTCAATG AAGATGGCTC CTTTATTGGC 
CAGTACACTG TCAAAAAGGA CAAGGAGGAA ACGGAGGGCA ATGAGAGCTC AGAGGCCACA 
TCACCAGTCA ATGCCATCTA TTCCCTTGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017320903.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176392.1 Mus musculus neurofascin (Nfasc), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OMu77145
Clone ID Related Accession (Same CDS sequence)	XM_017320903.2 , XM_017320903.1
Accession Version	XM_017320903.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320903.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGCA CAGAGTTGAC ATCCTCTGGA CATGGGTCAG AAGATACCTC CCCTCTGATT 
GGCAGAGAAA GGCACCTGGG TTTGATTCCA TCCAGAAAGC AGGTGTTTTA TAGTGTGTAC 
CTGGAGCCAC ACTGTCTCCA ATCAAATCCA GTCTCCAGGA CATGCCACCA GGTGACACTA 
GCCCGAGGAG TTGCAGAAAG AACGCCCAGC TTCATGTATC CCCAGGGCAC ATCGAGCAGT 
CAGATGGTTC TCCGTGGCAT GGACCTGCTG CTGGAATGCA TTGCCTCTGG CGTCCCAACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTCCCAT CTAACAAGGC CAAGTTCGAG 
AACTTTAATA AGGCTCTGCG CATCACCAAT GTCTCTGAAG AGGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGCATC CGGCACACGA TCTCGGTGAG AGTAAAGGCT 
GCTCCATACT GGCTGGATGA GCCCAAGAAC CTGATCCTGG CTCCTGGGGA AGATGGGAGG 
CTGGTATGCC GAGCCAATGG GAACCCGAAG CCGACCGTGC AGTGGATGGT GAATGGAGAG 
CCTTTACAAT CGGCACCACC CAATCCCAAC CGTGAGGTAG CTGGAGACAC TATCATCTTC 
CGGGATACTC AGATCAGCAG CAGGGCAGTG TACCAATGTA ATACATCCAA TGAACATGGC 
TACCTGCTGG CCAATGCCTT CGTCAGCGTG TTAGATGTAC CCCCTCGGAT GCTGTCTGCC 
CGCAACCAGC TCATCAGGGT GATCCTTTAT AACCGGACAC GGCTGGACTG TCCGTTCTTT 
GGGTCTCCCA TCCCAACACT CCGATGGTTT AAGAATGGGC AAGGAAGCAA CCTGGATGGC 
GGTAACTACC ACGTCTACGA AAACGGCAGT CTAGAAATCA AGATGATTCG CAAAGAGGAC 
CAAGGCATCT ACACCTGTGT GGCCACCAAC ATCCTGGGCA AAGCCGAAAA TCAAGTCCGC 
CTGGAGGTCA AAGACCCCAC CAGGATCTAC AGGATGCCCG AGGACCAGGT GGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAACATGACC CCTCCTTGAA GCTCACAGTC 
TCCTGGCTGA AGGACGATGA GCCACTCTAC ATTGGAAACA GGATGAAGAA GGAAGATGAC 
TCCCTGACGA TCTTCGGAGT GGCAGAGCGG GACCAGGGCA GTTACACGTG TATGGCCAGC 
ACCGAGCTGG ACCAGGACCT GGCAAAGGCC TACCTCACTG TTCTAGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTACCCAAA GGGCGACCAG ACCGACCCAG GGACCTGGAG 
CTCACTGACC TGGCTGAGAG GAGTGTGAGG CTGACCTGGA TCCCAGGGGA TGACAACAAC 
AGCCCTATCA CAGACTACGT CGTTCAGTTT GAAGAGGACC AGTTCCAACC AGGGGTGTGG 
CATGACCACT CCAGGTTCCC AGGCAGCGTC AACTCAGCCG TCCTCCATCT GTCCCCATAT 
GTCAACTACC AATTCAGAGT CATCGCTGTC AACGAGGTTG GGAGCAGCCA CCCCAGCCTT 
CCATCCGAGC GGTACCGAAC CAGTGGGGCA CCCCCTGAAT CTAATCCCAG TGATGTGAAG 
GGCGAAGGGA CAAGAAAGAA CAATATGGAG ATCACGTGGA CGCCTATGAA TGCTACCTCT 
GCCTTTGGCC CCAACCTACG CTACATTGTC AAGTGGCGAC GGAGAGAGAC CCGAGAGACT 
TGGAACAATG TCACAGTGTG GGGCTCTCGC TACGTGGTGG GGCAGACGCC TGTCTACGTT 
CCCTATGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AAGGCCCCGA GCCTGACACC 
ATCATTGGGT ACTCCGGAGA AGATTATCCC AGGGCTGCGC CCACTGAAGT TAAAATCCGA 
GTCCTGAACA GCACAGCCAT CAGCCTTCAG TGGAACCGAG TCTACTCTGA CACGGTCCAG 
GGCCAGCTCA GAGAGTATCG AGCTTACTAC TGGAGGGAAA GCAGTTTGCT GAAGAACCTG 
TGGGTGTCTC AGAAGAGACA GCAGGCCAGC TTCCCTGGTG ACCGTCCCCG GGGCGTGGTG 
GCCCGCCTGT TCCCCTACAG TAACTACAAG CTGGAGATGG TGGTGGTCAA TGGGAGAGGT 
GACGGGCCTC GAAGTGAAAC CAAGGAATTC ACCACCCCAG AAGGAGTACC CAGTGCCCCC 
AGGCGGTTCA GAGTCCGACA GCCCAACCTG GAGACCATCA ACCTGGAGTG GGACCACCCA 
GAGCACCCCA ACGGAATCCT GATTGGATAC ATCCTCAGAT ACGTGCCCTT TAATGGAACC 
AAACTGGGAA AGCAGATGGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CTCTGTGCAG 
AGAGCAGACC CAGTGTCGCG TTACCGCTTC TCCCTCAGTG CCAGGACACA GGTGGGCTCT 
GGAGAAGCAG CCACAGAGGA GTCCCCAGCA CCTCCAAATG AAGCTACTCC AACTGCAGCT 
CCTCCCACGT TGCCCCCGAC TACTGTGGGT ACCACAGGCC TTGTGAGCAG TACTGATGCT 
ACTGCCCTTG CTGCCACCAG TGAAGCCACA ACAGTTCCCA TCATTCCAAC CGTCGTACCT 
ACCACCGTCG CCACCACCAT TGCCACAACT ACTACAACCA CTGCCGCCAC CACCACCACC 
ACCACTACGG AGAGCCCTCC CACTACCACT GCTGGGACTA AGATTCACGA AACCGCCCCC 
GACGAGCAGT CCATTTGGAA CGTCACAGTG CTCCCCAACA GTAAATGGGC CAACATCACC 
TGGAAGCACA ATTTCAGGCC TGGAACTGAC TTTGTGGTTG AGTACATCGA CAGCAACCAT 
ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG GCCCAGCCTA TACAGCTGAC AGACCTCTTT 
CCCGGGATGA CGTACACGTT GCGGGTGTAT TCCCGGGACA ACGAGGGCAT CAGCAGTACC 
GTCATCACCT TTATGACCAG TACAGCTTAC ACCAATAACC AGGCAGACAT CGCCACCCAG 
GGCTGGTTCA TCGGGCTCAT GTGTGCCATT GCCCTTCTGG TGCTGATCCT TCTCATCGTC 
TGCTTCATCA AGAGGAGTCG AGGTGGCAAG TACCCAGTGC GGGAAAAGAA GGATGTCCCC 
TTGGGTCCTG AAGACCCCAA AGAAGAAGAT GGCTCATTTG ACTACAGTGA TGAGGACAAC 
AAGCCCCTGC AGGGCAGCCA GACATCTCTG GATGGCACCA TCAAGCAGCA GGAGAGCGAT 
GACAGCCTGG TGGACTATGG CGAAGGCGGC GAGGGCCAGT TCAATGAAGA TGGCTCCTTT 
ATTGGCCAGT ACACTGTCAA AAAGGACAAG GAGGAAACGG AGGGCAATGA GAGCTCAGAG 
GCCACATCAC CAGTCAATGC CATCTATTCC CTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176392.1
CDS	1423..4941
Translation	MICTELTSSGHGSEDTSPLIGRERHLGLIPSRKQVFYSVYLEPHCLQSNPVSRTCHQVTLARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017320903.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320903.2

ORF Insert Sequence:

ATGATCTGCA CAGAGTTGAC ATCCTCTGGA CATGGGTCAG AAGATACCTC CCCTCTGATT 
GGCAGAGAAA GGCACCTGGG TTTGATTCCA TCCAGAAAGC AGGTGTTTTA TAGTGTGTAC 
CTGGAGCCAC ACTGTCTCCA ATCAAATCCA GTCTCCAGGA CATGCCACCA GGTGACACTA 
GCCCGAGGAG TTGCAGAAAG AACGCCCAGC TTCATGTATC CCCAGGGCAC ATCGAGCAGT 
CAGATGGTTC TCCGTGGCAT GGACCTGCTG CTGGAATGCA TTGCCTCTGG CGTCCCAACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTCCCAT CTAACAAGGC CAAGTTCGAG 
AACTTTAATA AGGCTCTGCG CATCACCAAT GTCTCTGAAG AGGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGCATC CGGCACACGA TCTCGGTGAG AGTAAAGGCT 
GCTCCATACT GGCTGGATGA GCCCAAGAAC CTGATCCTGG CTCCTGGGGA AGATGGGAGG 
CTGGTATGCC GAGCCAATGG GAACCCGAAG CCGACCGTGC AGTGGATGGT GAATGGAGAG 
CCTTTACAAT CGGCACCACC CAATCCCAAC CGTGAGGTAG CTGGAGACAC TATCATCTTC 
CGGGATACTC AGATCAGCAG CAGGGCAGTG TACCAATGTA ATACATCCAA TGAACATGGC 
TACCTGCTGG CCAATGCCTT CGTCAGCGTG TTAGATGTAC CCCCTCGGAT GCTGTCTGCC 
CGCAACCAGC TCATCAGGGT GATCCTTTAT AACCGGACAC GGCTGGACTG TCCGTTCTTT 
GGGTCTCCCA TCCCAACACT CCGATGGTTT AAGAATGGGC AAGGAAGCAA CCTGGATGGC 
GGTAACTACC ACGTCTACGA AAACGGCAGT CTAGAAATCA AGATGATTCG CAAAGAGGAC 
CAAGGCATCT ACACCTGTGT GGCCACCAAC ATCCTGGGCA AAGCCGAAAA TCAAGTCCGC 
CTGGAGGTCA AAGACCCCAC CAGGATCTAC AGGATGCCCG AGGACCAGGT GGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAACATGACC CCTCCTTGAA GCTCACAGTC 
TCCTGGCTGA AGGACGATGA GCCACTCTAC ATTGGAAACA GGATGAAGAA GGAAGATGAC 
TCCCTGACGA TCTTCGGAGT GGCAGAGCGG GACCAGGGCA GTTACACGTG TATGGCCAGC 
ACCGAGCTGG ACCAGGACCT GGCAAAGGCC TACCTCACTG TTCTAGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTACCCAAA GGGCGACCAG ACCGACCCAG GGACCTGGAG 
CTCACTGACC TGGCTGAGAG GAGTGTGAGG CTGACCTGGA TCCCAGGGGA TGACAACAAC 
AGCCCTATCA CAGACTACGT CGTTCAGTTT GAAGAGGACC AGTTCCAACC AGGGGTGTGG 
CATGACCACT CCAGGTTCCC AGGCAGCGTC AACTCAGCCG TCCTCCATCT GTCCCCATAT 
GTCAACTACC AATTCAGAGT CATCGCTGTC AACGAGGTTG GGAGCAGCCA CCCCAGCCTT 
CCATCCGAGC GGTACCGAAC CAGTGGGGCA CCCCCTGAAT CTAATCCCAG TGATGTGAAG 
GGCGAAGGGA CAAGAAAGAA CAATATGGAG ATCACGTGGA CGCCTATGAA TGCTACCTCT 
GCCTTTGGCC CCAACCTACG CTACATTGTC AAGTGGCGAC GGAGAGAGAC CCGAGAGACT 
TGGAACAATG TCACAGTGTG GGGCTCTCGC TACGTGGTGG GGCAGACGCC TGTCTACGTT 
CCCTATGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AAGGCCCCGA GCCTGACACC 
ATCATTGGGT ACTCCGGAGA AGATTATCCC AGGGCTGCGC CCACTGAAGT TAAAATCCGA 
GTCCTGAACA GCACAGCCAT CAGCCTTCAG TGGAACCGAG TCTACTCTGA CACGGTCCAG 
GGCCAGCTCA GAGAGTATCG AGCTTACTAC TGGAGGGAAA GCAGTTTGCT GAAGAACCTG 
TGGGTGTCTC AGAAGAGACA GCAGGCCAGC TTCCCTGGTG ACCGTCCCCG GGGCGTGGTG 
GCCCGCCTGT TCCCCTACAG TAACTACAAG CTGGAGATGG TGGTGGTCAA TGGGAGAGGT 
GACGGGCCTC GAAGTGAAAC CAAGGAATTC ACCACCCCAG AAGGAGTACC CAGTGCCCCC 
AGGCGGTTCA GAGTCCGACA GCCCAACCTG GAGACCATCA ACCTGGAGTG GGACCACCCA 
GAGCACCCCA ACGGAATCCT GATTGGATAC ATCCTCAGAT ACGTGCCCTT TAATGGAACC 
AAACTGGGAA AGCAGATGGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CTCTGTGCAG 
AGAGCAGACC CAGTGTCGCG TTACCGCTTC TCCCTCAGTG CCAGGACACA GGTGGGCTCT 
GGAGAAGCAG CCACAGAGGA GTCCCCAGCA CCTCCAAATG AAGCTACTCC AACTGCAGCT 
CCTCCCACGT TGCCCCCGAC TACTGTGGGT ACCACAGGCC TTGTGAGCAG TACTGATGCT 
ACTGCCCTTG CTGCCACCAG TGAAGCCACA ACAGTTCCCA TCATTCCAAC CGTCGTACCT 
ACCACCGTCG CCACCACCAT TGCCACAACT ACTACAACCA CTGCCGCCAC CACCACCACC 
ACCACTACGG AGAGCCCTCC CACTACCACT GCTGGGACTA AGATTCACGA AACCGCCCCC 
GACGAGCAGT CCATTTGGAA CGTCACAGTG CTCCCCAACA GTAAATGGGC CAACATCACC 
TGGAAGCACA ATTTCAGGCC TGGAACTGAC TTTGTGGTTG AGTACATCGA CAGCAACCAT 
ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG GCCCAGCCTA TACAGCTGAC AGACCTCTTT 
CCCGGGATGA CGTACACGTT GCGGGTGTAT TCCCGGGACA ACGAGGGCAT CAGCAGTACC 
GTCATCACCT TTATGACCAG TACAGCTTAC ACCAATAACC AGGCAGACAT CGCCACCCAG 
GGCTGGTTCA TCGGGCTCAT GTGTGCCATT GCCCTTCTGG TGCTGATCCT TCTCATCGTC 
TGCTTCATCA AGAGGAGTCG AGGTGGCAAG TACCCAGTGC GGGAAAAGAA GGATGTCCCC 
TTGGGTCCTG AAGACCCCAA AGAAGAAGAT GGCTCATTTG ACTACAGTGA TGAGGACAAC 
AAGCCCCTGC AGGGCAGCCA GACATCTCTG GATGGCACCA TCAAGCAGCA GGAGAGCGAT 
GACAGCCTGG TGGACTATGG CGAAGGCGGC GAGGGCCAGT TCAATGAAGA TGGCTCCTTT 
ATTGGCCAGT ACACTGTCAA AAAGGACAAG GAGGAAACGG AGGGCAATGA GAGCTCAGAG 
GCCACATCAC CAGTCAATGC CATCTATTCC CTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017320903.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176392.1 Mus musculus neurofascin (Nfasc), transcript variant X30, mRNA.

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CloneID	OMu77145
Clone ID Related Accession (Same CDS sequence)	XM_017320903.2 , XM_017320903.1
Accession Version	XM_017320903.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGCA CAGAGTTGAC ATCCTCTGGA CATGGGTCAG AAGATACCTC CCCTCTGATT 
GGCAGAGAAA GGCACCTGGG TTTGATTCCA TCCAGAAAGC AGGTGTTTTA TAGTGTGTAC 
CTGGAGCCAC ACTGTCTCCA ATCAAATCCA GTCTCCAGGA CATGCCACCA GGTGACACTA 
GCCCGAGGAG TTGCAGAAAG AACGCCCAGC TTCATGTATC CCCAGGGCAC ATCGAGCAGT 
CAGATGGTTC TCCGTGGCAT GGACCTGCTG CTGGAATGCA TTGCCTCTGG CGTCCCAACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTCCCAT CTAACAAGGC CAAGTTCGAG 
AACTTTAATA AGGCTCTGCG CATCACCAAT GTCTCTGAAG AGGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGCATC CGGCACACGA TCTCGGTGAG AGTAAAGGCT 
GCTCCATACT GGCTGGATGA GCCCAAGAAC CTGATCCTGG CTCCTGGGGA AGATGGGAGG 
CTGGTATGCC GAGCCAATGG GAACCCGAAG CCGACCGTGC AGTGGATGGT GAATGGAGAG 
CCTTTACAAT CGGCACCACC CAATCCCAAC CGTGAGGTAG CTGGAGACAC TATCATCTTC 
CGGGATACTC AGATCAGCAG CAGGGCAGTG TACCAATGTA ATACATCCAA TGAACATGGC 
TACCTGCTGG CCAATGCCTT CGTCAGCGTG TTAGATGTAC CCCCTCGGAT GCTGTCTGCC 
CGCAACCAGC TCATCAGGGT GATCCTTTAT AACCGGACAC GGCTGGACTG TCCGTTCTTT 
GGGTCTCCCA TCCCAACACT CCGATGGTTT AAGAATGGGC AAGGAAGCAA CCTGGATGGC 
GGTAACTACC ACGTCTACGA AAACGGCAGT CTAGAAATCA AGATGATTCG CAAAGAGGAC 
CAAGGCATCT ACACCTGTGT GGCCACCAAC ATCCTGGGCA AAGCCGAAAA TCAAGTCCGC 
CTGGAGGTCA AAGACCCCAC CAGGATCTAC AGGATGCCCG AGGACCAGGT GGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAACATGACC CCTCCTTGAA GCTCACAGTC 
TCCTGGCTGA AGGACGATGA GCCACTCTAC ATTGGAAACA GGATGAAGAA GGAAGATGAC 
TCCCTGACGA TCTTCGGAGT GGCAGAGCGG GACCAGGGCA GTTACACGTG TATGGCCAGC 
ACCGAGCTGG ACCAGGACCT GGCAAAGGCC TACCTCACTG TTCTAGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTACCCAAA GGGCGACCAG ACCGACCCAG GGACCTGGAG 
CTCACTGACC TGGCTGAGAG GAGTGTGAGG CTGACCTGGA TCCCAGGGGA TGACAACAAC 
AGCCCTATCA CAGACTACGT CGTTCAGTTT GAAGAGGACC AGTTCCAACC AGGGGTGTGG 
CATGACCACT CCAGGTTCCC AGGCAGCGTC AACTCAGCCG TCCTCCATCT GTCCCCATAT 
GTCAACTACC AATTCAGAGT CATCGCTGTC AACGAGGTTG GGAGCAGCCA CCCCAGCCTT 
CCATCCGAGC GGTACCGAAC CAGTGGGGCA CCCCCTGAAT CTAATCCCAG TGATGTGAAG 
GGCGAAGGGA CAAGAAAGAA CAATATGGAG ATCACGTGGA CGCCTATGAA TGCTACCTCT 
GCCTTTGGCC CCAACCTACG CTACATTGTC AAGTGGCGAC GGAGAGAGAC CCGAGAGACT 
TGGAACAATG TCACAGTGTG GGGCTCTCGC TACGTGGTGG GGCAGACGCC TGTCTACGTT 
CCCTATGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AAGGCCCCGA GCCTGACACC 
ATCATTGGGT ACTCCGGAGA AGATTATCCC AGGGCTGCGC CCACTGAAGT TAAAATCCGA 
GTCCTGAACA GCACAGCCAT CAGCCTTCAG TGGAACCGAG TCTACTCTGA CACGGTCCAG 
GGCCAGCTCA GAGAGTATCG AGCTTACTAC TGGAGGGAAA GCAGTTTGCT GAAGAACCTG 
TGGGTGTCTC AGAAGAGACA GCAGGCCAGC TTCCCTGGTG ACCGTCCCCG GGGCGTGGTG 
GCCCGCCTGT TCCCCTACAG TAACTACAAG CTGGAGATGG TGGTGGTCAA TGGGAGAGGT 
GACGGGCCTC GAAGTGAAAC CAAGGAATTC ACCACCCCAG AAGGAGTACC CAGTGCCCCC 
AGGCGGTTCA GAGTCCGACA GCCCAACCTG GAGACCATCA ACCTGGAGTG GGACCACCCA 
GAGCACCCCA ACGGAATCCT GATTGGATAC ATCCTCAGAT ACGTGCCCTT TAATGGAACC 
AAACTGGGAA AGCAGATGGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CTCTGTGCAG 
AGAGCAGACC CAGTGTCGCG TTACCGCTTC TCCCTCAGTG CCAGGACACA GGTGGGCTCT 
GGAGAAGCAG CCACAGAGGA GTCCCCAGCA CCTCCAAATG AAGCTACTCC AACTGCAGCT 
CCTCCCACGT TGCCCCCGAC TACTGTGGGT ACCACAGGCC TTGTGAGCAG TACTGATGCT 
ACTGCCCTTG CTGCCACCAG TGAAGCCACA ACAGTTCCCA TCATTCCAAC CGTCGTACCT 
ACCACCGTCG CCACCACCAT TGCCACAACT ACTACAACCA CTGCCGCCAC CACCACCACC 
ACCACTACGG AGAGCCCTCC CACTACCACT GCTGGGACTA AGATTCACGA AACCGCCCCC 
GACGAGCAGT CCATTTGGAA CGTCACAGTG CTCCCCAACA GTAAATGGGC CAACATCACC 
TGGAAGCACA ATTTCAGGCC TGGAACTGAC TTTGTGGTTG AGTACATCGA CAGCAACCAT 
ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG GCCCAGCCTA TACAGCTGAC AGACCTCTTT 
CCCGGGATGA CGTACACGTT GCGGGTGTAT TCCCGGGACA ACGAGGGCAT CAGCAGTACC 
GTCATCACCT TTATGACCAG TACAGCTTAC ACCAATAACC AGGCAGACAT CGCCACCCAG 
GGCTGGTTCA TCGGGCTCAT GTGTGCCATT GCCCTTCTGG TGCTGATCCT TCTCATCGTC 
TGCTTCATCA AGAGGAGTCG AGGTGGCAAG TACCCAGTGC GGGAAAAGAA GGATGTCCCC 
TTGGGTCCTG AAGACCCCAA AGAAGAAGAT GGCTCATTTG ACTACAGTGA TGAGGACAAC 
AAGCCCCTGC AGGGCAGCCA GACATCTCTG GATGGCACCA TCAAGCAGCA GGAGAGCGAT 
GACAGCCTGG TGGACTATGG CGAAGGCGGC GAGGGCCAGT TCAATGAAGA TGGCTCCTTT 
ATTGGCCAGT ACACTGTCAA AAAGGACAAG GAGGAAACGG AGGGCAATGA GAGCTCAGAG 
GCCACATCAC CAGTCAATGC CATCTATTCC CTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176392.1
CDS	1387..4905
Translation	MICTELTSSGHGSEDTSPLIGRERHLGLIPSRKQVFYSVYLEPHCLQSNPVSRTCHQVTLARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017320903.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320903.1

ORF Insert Sequence:

ATGATCTGCA CAGAGTTGAC ATCCTCTGGA CATGGGTCAG AAGATACCTC CCCTCTGATT 
GGCAGAGAAA GGCACCTGGG TTTGATTCCA TCCAGAAAGC AGGTGTTTTA TAGTGTGTAC 
CTGGAGCCAC ACTGTCTCCA ATCAAATCCA GTCTCCAGGA CATGCCACCA GGTGACACTA 
GCCCGAGGAG TTGCAGAAAG AACGCCCAGC TTCATGTATC CCCAGGGCAC ATCGAGCAGT 
CAGATGGTTC TCCGTGGCAT GGACCTGCTG CTGGAATGCA TTGCCTCTGG CGTCCCAACA 
CCAGACATTG CATGGTACAA GAAAGGTGGG GACCTCCCAT CTAACAAGGC CAAGTTCGAG 
AACTTTAATA AGGCTCTGCG CATCACCAAT GTCTCTGAAG AGGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGCATC CGGCACACGA TCTCGGTGAG AGTAAAGGCT 
GCTCCATACT GGCTGGATGA GCCCAAGAAC CTGATCCTGG CTCCTGGGGA AGATGGGAGG 
CTGGTATGCC GAGCCAATGG GAACCCGAAG CCGACCGTGC AGTGGATGGT GAATGGAGAG 
CCTTTACAAT CGGCACCACC CAATCCCAAC CGTGAGGTAG CTGGAGACAC TATCATCTTC 
CGGGATACTC AGATCAGCAG CAGGGCAGTG TACCAATGTA ATACATCCAA TGAACATGGC 
TACCTGCTGG CCAATGCCTT CGTCAGCGTG TTAGATGTAC CCCCTCGGAT GCTGTCTGCC 
CGCAACCAGC TCATCAGGGT GATCCTTTAT AACCGGACAC GGCTGGACTG TCCGTTCTTT 
GGGTCTCCCA TCCCAACACT CCGATGGTTT AAGAATGGGC AAGGAAGCAA CCTGGATGGC 
GGTAACTACC ACGTCTACGA AAACGGCAGT CTAGAAATCA AGATGATTCG CAAAGAGGAC 
CAAGGCATCT ACACCTGTGT GGCCACCAAC ATCCTGGGCA AAGCCGAAAA TCAAGTCCGC 
CTGGAGGTCA AAGACCCCAC CAGGATCTAC AGGATGCCCG AGGACCAGGT GGCCAAGAGG 
GGCACCACGG TGCAGCTGGA GTGCCGCGTG AAACATGACC CCTCCTTGAA GCTCACAGTC 
TCCTGGCTGA AGGACGATGA GCCACTCTAC ATTGGAAACA GGATGAAGAA GGAAGATGAC 
TCCCTGACGA TCTTCGGAGT GGCAGAGCGG GACCAGGGCA GTTACACGTG TATGGCCAGC 
ACCGAGCTGG ACCAGGACCT GGCAAAGGCC TACCTCACTG TTCTAGCTGA TCAGGCCACT 
CCAACTAACC GTTTGGCTGC CCTACCCAAA GGGCGACCAG ACCGACCCAG GGACCTGGAG 
CTCACTGACC TGGCTGAGAG GAGTGTGAGG CTGACCTGGA TCCCAGGGGA TGACAACAAC 
AGCCCTATCA CAGACTACGT CGTTCAGTTT GAAGAGGACC AGTTCCAACC AGGGGTGTGG 
CATGACCACT CCAGGTTCCC AGGCAGCGTC AACTCAGCCG TCCTCCATCT GTCCCCATAT 
GTCAACTACC AATTCAGAGT CATCGCTGTC AACGAGGTTG GGAGCAGCCA CCCCAGCCTT 
CCATCCGAGC GGTACCGAAC CAGTGGGGCA CCCCCTGAAT CTAATCCCAG TGATGTGAAG 
GGCGAAGGGA CAAGAAAGAA CAATATGGAG ATCACGTGGA CGCCTATGAA TGCTACCTCT 
GCCTTTGGCC CCAACCTACG CTACATTGTC AAGTGGCGAC GGAGAGAGAC CCGAGAGACT 
TGGAACAATG TCACAGTGTG GGGCTCTCGC TACGTGGTGG GGCAGACGCC TGTCTACGTT 
CCCTATGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AAGGCCCCGA GCCTGACACC 
ATCATTGGGT ACTCCGGAGA AGATTATCCC AGGGCTGCGC CCACTGAAGT TAAAATCCGA 
GTCCTGAACA GCACAGCCAT CAGCCTTCAG TGGAACCGAG TCTACTCTGA CACGGTCCAG 
GGCCAGCTCA GAGAGTATCG AGCTTACTAC TGGAGGGAAA GCAGTTTGCT GAAGAACCTG 
TGGGTGTCTC AGAAGAGACA GCAGGCCAGC TTCCCTGGTG ACCGTCCCCG GGGCGTGGTG 
GCCCGCCTGT TCCCCTACAG TAACTACAAG CTGGAGATGG TGGTGGTCAA TGGGAGAGGT 
GACGGGCCTC GAAGTGAAAC CAAGGAATTC ACCACCCCAG AAGGAGTACC CAGTGCCCCC 
AGGCGGTTCA GAGTCCGACA GCCCAACCTG GAGACCATCA ACCTGGAGTG GGACCACCCA 
GAGCACCCCA ACGGAATCCT GATTGGATAC ATCCTCAGAT ACGTGCCCTT TAATGGAACC 
AAACTGGGAA AGCAGATGGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CTCTGTGCAG 
AGAGCAGACC CAGTGTCGCG TTACCGCTTC TCCCTCAGTG CCAGGACACA GGTGGGCTCT 
GGAGAAGCAG CCACAGAGGA GTCCCCAGCA CCTCCAAATG AAGCTACTCC AACTGCAGCT 
CCTCCCACGT TGCCCCCGAC TACTGTGGGT ACCACAGGCC TTGTGAGCAG TACTGATGCT 
ACTGCCCTTG CTGCCACCAG TGAAGCCACA ACAGTTCCCA TCATTCCAAC CGTCGTACCT 
ACCACCGTCG CCACCACCAT TGCCACAACT ACTACAACCA CTGCCGCCAC CACCACCACC 
ACCACTACGG AGAGCCCTCC CACTACCACT GCTGGGACTA AGATTCACGA AACCGCCCCC 
GACGAGCAGT CCATTTGGAA CGTCACAGTG CTCCCCAACA GTAAATGGGC CAACATCACC 
TGGAAGCACA ATTTCAGGCC TGGAACTGAC TTTGTGGTTG AGTACATCGA CAGCAACCAT 
ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG GCCCAGCCTA TACAGCTGAC AGACCTCTTT 
CCCGGGATGA CGTACACGTT GCGGGTGTAT TCCCGGGACA ACGAGGGCAT CAGCAGTACC 
GTCATCACCT TTATGACCAG TACAGCTTAC ACCAATAACC AGGCAGACAT CGCCACCCAG 
GGCTGGTTCA TCGGGCTCAT GTGTGCCATT GCCCTTCTGG TGCTGATCCT TCTCATCGTC 
TGCTTCATCA AGAGGAGTCG AGGTGGCAAG TACCCAGTGC GGGAAAAGAA GGATGTCCCC 
TTGGGTCCTG AAGACCCCAA AGAAGAAGAT GGCTCATTTG ACTACAGTGA TGAGGACAAC 
AAGCCCCTGC AGGGCAGCCA GACATCTCTG GATGGCACCA TCAAGCAGCA GGAGAGCGAT 
GACAGCCTGG TGGACTATGG CGAAGGCGGC GAGGGCCAGT TCAATGAAGA TGGCTCCTTT 
ATTGGCCAGT ACACTGTCAA AAAGGACAAG GAGGAAACGG AGGGCAATGA GAGCTCAGAG 
GCCACATCAC CAGTCAATGC CATCTATTCC CTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017320911.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176400.1 Mus musculus neurofascin (Nfasc), transcript variant X36, mRNA.

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CloneID	OMu77148
Clone ID Related Accession (Same CDS sequence)	XM_017320911.1
Accession Version	XM_017320911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CACCCGAGGA 
GTTGCAGAAA GAACGCCCAG CTTCATGTAT CCCCAGGGCA CATCGAGCAG TCAGATGGTT 
CTCCGTGGCA TGGACCTGCT GCTGGAATGC ATTGCCTCTG GCGTCCCAAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTAACAAGG CCAAGTTCGA GAACTTTAAT 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCTCCATAC 
TGGCTGGATG AGCCCAAGAA CCTGATCCTG GCTCCTGGGG AAGATGGGAG GCTGGTATGC 
CGAGCCAATG GGAACCCGAA GCCGACCGTG CAGTGGATGG TGAATGGAGA GCCTTTACAA 
TCGGCACCAC CCAATCCCAA CCGTGAGGTA GCTGGAGACA CTATCATCTT CCGGGATACT 
CAGATCAGCA GCAGGGCAGT GTACCAATGT AATACATCCA ATGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT GTTAGATGTA CCCCCTCGGA TGCTGTCTGC CCGCAACCAG 
CTCATCAGGG TGATCCTTTA TAACCGGACA CGGCTGGACT GTCCGTTCTT TGGGTCTCCC 
ATCCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG CGGTAACTAC 
CACGTCTACG AAAACGGCAG TCTAGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGC AAAGCCGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCC GAGGACCAGG TGGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAACATGAC CCCTCCTTGA AGCTCACAGT CTCCTGGCTG 
AAGGACGATG AGCCACTCTA CATTGGAAAC AGGATGAAGA AGGAAGATGA CTCCCTGACG 
ATCTTCGGAG TGGCAGAGCG GGACCAGGGC AGTTACACGT GTATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTACCCAA AGGGCGACCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCTGAGA GGAGTGTGAG GCTGACCTGG ATCCCAGGGG ATGACAACAA CAGCCCTATC 
ACAGACTACG TCGTTCAGTT TGAAGAGGAC CAGTTCCAAC CAGGGGTGTG GCATGACCAC 
TCCAGGTTCC CAGGCAGCGT CAACTCAGCC GTCCTCCATC TGTCCCCATA TGTCAACTAC 
CAATTCAGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CCAGTGGGGC ACCCCCTGAA TCTAATCCCA GTGATGTGAA GGGCGAAGGG 
ACAAGAAAGA ACAATATGGA GATCACGTGG ACGCCTATGA ATGCTACCTC TGCCTTTGGC 
CCCAACCTAC GCTACATTGT CAAGTGGCGA CGGAGAGAGA CCCGAGAGAC TTGGAACAAT 
GTCACAGTGT GGGGCTCTCG CTACGTGGTG GGGCAGACGC CTGTCTACGT TCCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCCG AGCCTGACAC CATCATTGGG 
TACTCCGGAG AAGATTTACC CAGTGCCCCC AGGCGGTTCA GAGTCCGACA GCCCAACCTG 
GAGACCATCA ACCTGGAGTG GGACCACCCA GAGCACCCCA ACGGAATCCT GATTGGATAC 
ATCCTCAGAT ACGTGCCCTT TAATGGAACC AAACTGGGAA AGCAGATGGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CTCTGTGCAG AGAGCAGACC CAGTGTCGCG TTACCGCTTC 
TCCCTCAGTG CCAGGACACA GGTGGGCTCT GGAGAAGCAG CCACAGAGGA GTCCCCAGCA 
CCTCCAAATG AAGCTACTCC AACTGCAGCT TACACCAATA ACCAGGCAGA CATCGCCACC 
CAGGGCTGGT TCATCGGGCT CATGTGTGCC ATTGCCCTTC TGGTGCTGAT CCTTCTCATC 
GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC AAGTACCCAG TGCGGGAAAA GAAGGATGTC 
CCCTTGGGTC CTGAAGACCC CAAAGAAGAA GATGGCTCAT TTGACTACAG TGATGAGGAC 
AACAAGCCCC TGCAGGGCAG CCAGACATCT CTGGATGGCA CCATCAAGCA GCAGGAGAGC 
GATGACAGCC TGGTGGACTA TGGCGAAGGC GGCGAGGGCC AGTTCAATGA AGATGGCTCC 
TTTATTGGCC AGTACACTGT CAAAAAGGAC AAGGAGGAAA CGGAGGGCAA TGAGAGCTCA 
GAGGCCACAT CACCAGTCAA TGCCATCTAT TCCCTTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176400.1
CDS	52..3573
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017320911.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320911.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CACCCGAGGA 
GTTGCAGAAA GAACGCCCAG CTTCATGTAT CCCCAGGGCA CATCGAGCAG TCAGATGGTT 
CTCCGTGGCA TGGACCTGCT GCTGGAATGC ATTGCCTCTG GCGTCCCAAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTAACAAGG CCAAGTTCGA GAACTTTAAT 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCTCCATAC 
TGGCTGGATG AGCCCAAGAA CCTGATCCTG GCTCCTGGGG AAGATGGGAG GCTGGTATGC 
CGAGCCAATG GGAACCCGAA GCCGACCGTG CAGTGGATGG TGAATGGAGA GCCTTTACAA 
TCGGCACCAC CCAATCCCAA CCGTGAGGTA GCTGGAGACA CTATCATCTT CCGGGATACT 
CAGATCAGCA GCAGGGCAGT GTACCAATGT AATACATCCA ATGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT GTTAGATGTA CCCCCTCGGA TGCTGTCTGC CCGCAACCAG 
CTCATCAGGG TGATCCTTTA TAACCGGACA CGGCTGGACT GTCCGTTCTT TGGGTCTCCC 
ATCCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG CGGTAACTAC 
CACGTCTACG AAAACGGCAG TCTAGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGC AAAGCCGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCC GAGGACCAGG TGGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAACATGAC CCCTCCTTGA AGCTCACAGT CTCCTGGCTG 
AAGGACGATG AGCCACTCTA CATTGGAAAC AGGATGAAGA AGGAAGATGA CTCCCTGACG 
ATCTTCGGAG TGGCAGAGCG GGACCAGGGC AGTTACACGT GTATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTACCCAA AGGGCGACCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCTGAGA GGAGTGTGAG GCTGACCTGG ATCCCAGGGG ATGACAACAA CAGCCCTATC 
ACAGACTACG TCGTTCAGTT TGAAGAGGAC CAGTTCCAAC CAGGGGTGTG GCATGACCAC 
TCCAGGTTCC CAGGCAGCGT CAACTCAGCC GTCCTCCATC TGTCCCCATA TGTCAACTAC 
CAATTCAGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CCAGTGGGGC ACCCCCTGAA TCTAATCCCA GTGATGTGAA GGGCGAAGGG 
ACAAGAAAGA ACAATATGGA GATCACGTGG ACGCCTATGA ATGCTACCTC TGCCTTTGGC 
CCCAACCTAC GCTACATTGT CAAGTGGCGA CGGAGAGAGA CCCGAGAGAC TTGGAACAAT 
GTCACAGTGT GGGGCTCTCG CTACGTGGTG GGGCAGACGC CTGTCTACGT TCCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCCG AGCCTGACAC CATCATTGGG 
TACTCCGGAG AAGATTTACC CAGTGCCCCC AGGCGGTTCA GAGTCCGACA GCCCAACCTG 
GAGACCATCA ACCTGGAGTG GGACCACCCA GAGCACCCCA ACGGAATCCT GATTGGATAC 
ATCCTCAGAT ACGTGCCCTT TAATGGAACC AAACTGGGAA AGCAGATGGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CTCTGTGCAG AGAGCAGACC CAGTGTCGCG TTACCGCTTC 
TCCCTCAGTG CCAGGACACA GGTGGGCTCT GGAGAAGCAG CCACAGAGGA GTCCCCAGCA 
CCTCCAAATG AAGCTACTCC AACTGCAGCT TACACCAATA ACCAGGCAGA CATCGCCACC 
CAGGGCTGGT TCATCGGGCT CATGTGTGCC ATTGCCCTTC TGGTGCTGAT CCTTCTCATC 
GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC AAGTACCCAG TGCGGGAAAA GAAGGATGTC 
CCCTTGGGTC CTGAAGACCC CAAAGAAGAA GATGGCTCAT TTGACTACAG TGATGAGGAC 
AACAAGCCCC TGCAGGGCAG CCAGACATCT CTGGATGGCA CCATCAAGCA GCAGGAGAGC 
GATGACAGCC TGGTGGACTA TGGCGAAGGC GGCGAGGGCC AGTTCAATGA AGATGGCTCC 
TTTATTGGCC AGTACACTGT CAAAAAGGAC AAGGAGGAAA CGGAGGGCAA TGAGAGCTCA 
GAGGCCACAT CACCAGTCAA TGCCATCTAT TCCCTTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017320912.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176401.1 Mus musculus neurofascin (Nfasc), transcript variant X37, mRNA.

Price & Availability↑

CloneID	OMu77149
Clone ID Related Accession (Same CDS sequence)	XM_017320912.1
Accession Version	XM_017320912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CACCCGAGGA 
GTTGCAGAAA GAACGCCCAG CTTCATGTAT CCCCAGGGCA CATCGAGCAG TCAGATGGTT 
CTCCGTGGCA TGGACCTGCT GCTGGAATGC ATTGCCTCTG GCGTCCCAAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTAACAAGG CCAAGTTCGA GAACTTTAAT 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCTCCATAC 
TGGCTGGATG AGCCCAAGAA CCTGATCCTG GCTCCTGGGG AAGATGGGAG GCTGGTATGC 
CGAGCCAATG GGAACCCGAA GCCGACCGTG CAGTGGATGG TGAATGGAGA GCCTTTACAA 
TCGGCACCAC CCAATCCCAA CCGTGAGGTA GCTGGAGACA CTATCATCTT CCGGGATACT 
CAGATCAGCA GCAGGGCAGT GTACCAATGT AATACATCCA ATGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT GTTAGATGTA CCCCCTCGGA TGCTGTCTGC CCGCAACCAG 
CTCATCAGGG TGATCCTTTA TAACCGGACA CGGCTGGACT GTCCGTTCTT TGGGTCTCCC 
ATCCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG CGGTAACTAC 
CACGTCTACG AAAACGGCAG TCTAGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGC AAAGCCGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCC GAGGACCAGG TGGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAACATGAC CCCTCCTTGA AGCTCACAGT CTCCTGGCTG 
AAGGACGATG AGCCACTCTA CATTGGAAAC AGGATGAAGA AGGAAGATGA CTCCCTGACG 
ATCTTCGGAG TGGCAGAGCG GGACCAGGGC AGTTACACGT GTATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTACCCAA AGGGCGACCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCTGAGA GGAGTGTGAG GCTGACCTGG ATCCCAGGGG ATGACAACAA CAGCCCTATC 
ACAGACTACG TCGTTCAGTT TGAAGAGGAC CAGTTCCAAC CAGGGGTGTG GCATGACCAC 
TCCAGGTTCC CAGGCAGCGT CAACTCAGCC GTCCTCCATC TGTCCCCATA TGTCAACTAC 
CAATTCAGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CCAGTGGGGC ACCCCCTGAA TCTAATCCCA GTGATGTGAA GGGCGAAGGG 
ACAAGAAAGA ACAATATGGA GATCACGTGG ACGCCTATGA ATGCTACCTC TGCCTTTGGC 
CCCAACCTAC GCTACATTGT CAAGTGGCGA CGGAGAGAGA CCCGAGAGAC TTGGAACAAT 
GTCACAGTGT GGGGCTCTCG CTACGTGGTG GGGCAGACGC CTGTCTACGT TCCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCCG AGCCTGACAC CATCATTGGG 
TACTCCGGAG AAGATTTACC CAGTGCCCCC AGGCGGTTCA GAGTCCGACA GCCCAACCTG 
GAGACCATCA ACCTGGAGTG GGACCACCCA GAGCACCCCA ACGGAATCCT GATTGGATAC 
ATCCTCAGAT ACGTGCCCTT TAATGGAACC AAACTGGGAA AGCAGATGGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CTCTGTGCAG AGAGCAGACC CAGTGTCGCG TTACCGCTTC 
TCCCTCAGTG CCAGGACACA GGTGGGCTCT GGAGAAGCAG CCACAGAGGA GTCCCCAGCA 
CCTCCAAATG AAGCTTACAC CAATAACCAG GCAGACATCG CCACCCAGGG CTGGTTCATC 
GGGCTCATGT GTGCCATTGC CCTTCTGGTG CTGATCCTTC TCATCGTCTG CTTCATCAAG 
AGGAGTCGAG GTGGCAAGTA CCCAGTGCGG GAAAAGAAGG ATGTCCCCTT GGGTCCTGAA 
GACCCCAAAG AAGAAGATGG CTCATTTGAC TACAGTGATG AGGACAACAA GCCCCTGCAG 
GGCAGCCAGA CATCTCTGGA TGGCACCATC AAGCAGCAGG AGAGCGATGA CAGCCTGGTG 
GACTATGGCG AAGGCGGCGA GGGCCAGTTC AATGAAGATG GCTCCTTTAT TGGCCAGTAC 
ACTGTCAAAA AGGACAAGGA GGAAACGGAG GGCAATGAGA GCTCAGAGGC CACATCACCA 
GTCAATGCCA TCTATTCCCT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176401.1
CDS	52..3558
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017320912.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320912.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CACCCGAGGA 
GTTGCAGAAA GAACGCCCAG CTTCATGTAT CCCCAGGGCA CATCGAGCAG TCAGATGGTT 
CTCCGTGGCA TGGACCTGCT GCTGGAATGC ATTGCCTCTG GCGTCCCAAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTAACAAGG CCAAGTTCGA GAACTTTAAT 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCTCCATAC 
TGGCTGGATG AGCCCAAGAA CCTGATCCTG GCTCCTGGGG AAGATGGGAG GCTGGTATGC 
CGAGCCAATG GGAACCCGAA GCCGACCGTG CAGTGGATGG TGAATGGAGA GCCTTTACAA 
TCGGCACCAC CCAATCCCAA CCGTGAGGTA GCTGGAGACA CTATCATCTT CCGGGATACT 
CAGATCAGCA GCAGGGCAGT GTACCAATGT AATACATCCA ATGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT GTTAGATGTA CCCCCTCGGA TGCTGTCTGC CCGCAACCAG 
CTCATCAGGG TGATCCTTTA TAACCGGACA CGGCTGGACT GTCCGTTCTT TGGGTCTCCC 
ATCCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG CGGTAACTAC 
CACGTCTACG AAAACGGCAG TCTAGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGC AAAGCCGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCC GAGGACCAGG TGGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAACATGAC CCCTCCTTGA AGCTCACAGT CTCCTGGCTG 
AAGGACGATG AGCCACTCTA CATTGGAAAC AGGATGAAGA AGGAAGATGA CTCCCTGACG 
ATCTTCGGAG TGGCAGAGCG GGACCAGGGC AGTTACACGT GTATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTACCCAA AGGGCGACCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCTGAGA GGAGTGTGAG GCTGACCTGG ATCCCAGGGG ATGACAACAA CAGCCCTATC 
ACAGACTACG TCGTTCAGTT TGAAGAGGAC CAGTTCCAAC CAGGGGTGTG GCATGACCAC 
TCCAGGTTCC CAGGCAGCGT CAACTCAGCC GTCCTCCATC TGTCCCCATA TGTCAACTAC 
CAATTCAGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CCAGTGGGGC ACCCCCTGAA TCTAATCCCA GTGATGTGAA GGGCGAAGGG 
ACAAGAAAGA ACAATATGGA GATCACGTGG ACGCCTATGA ATGCTACCTC TGCCTTTGGC 
CCCAACCTAC GCTACATTGT CAAGTGGCGA CGGAGAGAGA CCCGAGAGAC TTGGAACAAT 
GTCACAGTGT GGGGCTCTCG CTACGTGGTG GGGCAGACGC CTGTCTACGT TCCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCCG AGCCTGACAC CATCATTGGG 
TACTCCGGAG AAGATTTACC CAGTGCCCCC AGGCGGTTCA GAGTCCGACA GCCCAACCTG 
GAGACCATCA ACCTGGAGTG GGACCACCCA GAGCACCCCA ACGGAATCCT GATTGGATAC 
ATCCTCAGAT ACGTGCCCTT TAATGGAACC AAACTGGGAA AGCAGATGGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CTCTGTGCAG AGAGCAGACC CAGTGTCGCG TTACCGCTTC 
TCCCTCAGTG CCAGGACACA GGTGGGCTCT GGAGAAGCAG CCACAGAGGA GTCCCCAGCA 
CCTCCAAATG AAGCTTACAC CAATAACCAG GCAGACATCG CCACCCAGGG CTGGTTCATC 
GGGCTCATGT GTGCCATTGC CCTTCTGGTG CTGATCCTTC TCATCGTCTG CTTCATCAAG 
AGGAGTCGAG GTGGCAAGTA CCCAGTGCGG GAAAAGAAGG ATGTCCCCTT GGGTCCTGAA 
GACCCCAAAG AAGAAGATGG CTCATTTGAC TACAGTGATG AGGACAACAA GCCCCTGCAG 
GGCAGCCAGA CATCTCTGGA TGGCACCATC AAGCAGCAGG AGAGCGATGA CAGCCTGGTG 
GACTATGGCG AAGGCGGCGA GGGCCAGTTC AATGAAGATG GCTCCTTTAT TGGCCAGTAC 
ACTGTCAAAA AGGACAAGGA GGAAACGGAG GGCAATGAGA GCTCAGAGGC CACATCACCA 
GTCAATGCCA TCTATTCCCT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_030254517.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110377.1 Mus musculus neurofascin (Nfasc), transcript variant X5, mRNA.

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CloneID	OMu85168
Clone ID Related Accession (Same CDS sequence)	XM_030254517.1
Accession Version	XM_030254517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG GGCGACCAGA CCGACCCAGG GACCTGGAGC TCACTGACCT GGCTGAGAGG 
AGTGTGAGGC TGACCTGGAT CCCAGGGGAT GACAACAACA GCCCTATCAC AGACTACGTC 
GTTCAGTTTG AAGAGGACCA GTTCCAACCA GGGGTGTGGC ATGACCACTC CAGGTTCCCA 
GGCAGCGTCA ACTCAGCCGT CCTCCATCTG TCCCCATATG TCAACTACCA ATTCAGAGTC 
ATCGCTGTCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTTC CATCCGAGCG GTACCGAACC 
AGTGGGGCAC CCCCTGAATC TAATCCCAGT GATGTGAAGG GCGAAGGGAC AAGAAAGAAC 
AATATGGAGA TCACGTGGAC GCCTATGAAT GCTACCTCTG CCTTTGGCCC CAACCTACGC 
TACATTGTCA AGTGGCGACG GAGAGAGACC CGAGAGACTT GGAACAATGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACGCCT GTCTACGTTC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTTGGGAA AGGCCCCGAG CCTGACACCA TCATTGGGTA CTCCGGAGAA 
GATTATCCCA GGGCTGCGCC CACTGAAGTT AAAATCCGAG TCCTGAACAG CACAGCCATC 
AGCCTTCAGT GGAACCGAGT CTACTCTGAC ACGGTCCAGG GCCAGCTCAG AGAGTATCGA 
GCTTACTACT GGAGGGAAAG CAGTTTGCTG AAGAACCTGT GGGTGTCTCA GAAGAGACAG 
CAGGCCAGCT TCCCTGGTGA CCGTCCCCGG GGCGTGGTGG CCCGCCTGTT CCCCTACAGT 
AACTACAAGC TGGAGATGGT GGTGGTCAAT GGGAGAGGTG ACGGGCCTCG AAGTGAAACC 
AAGGAATTCA CCACCCCAGA AGGAGTACCC AGTGCCCCCA GGCGGTTCAG AGTCCGACAG 
CCCAACCTGG AGACCATCAA CCTGGAGTGG GACCACCCAG AGCACCCCAA CGGAATCCTG 
ATTGGATACA TCCTCAGATA CGTGCCCTTT AATGGAACCA AACTGGGAAA GCAGATGGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC TCTGTGCAGA GAGCAGACCC AGTGTCGCGT 
TACCGCTTCT CCCTCAGTGC CAGGACACAG GTGGGCTCTG GAGAAGCAGC CACAGAGGAG 
TCCCCAGCAC CTCCAAATGA AGCTACTCCA ACTGCAGCTC CTCCCACGTT GCCCCCGACT 
ACTGTGGGTA CCACAGGCCT TGTGAGCAGT ACTGATGCTA CTGCCCTTGC TGCCACCAGT 
GAAGCCACAA CAGTTCCCAT CATTCCAACC GTCGTACCTA CCACCGTCGC CACCACCATT 
GCCACAACTA CTACAACCAC TGCCGCCACC ACCACCACCA CCACTACGGA GAGCCCTCCC 
ACTACCACTG CTGGGACTAA GATTCACGAA ACCGCCCCCG ACGAGCAGTC CATTTGGAAC 
GTCACAGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAAGCACAA TTTCAGGCCT 
GGAACTGACT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCTGTT 
AAGGCCCAGG CCCAGCCTAT ACAGCTGACA GACCTCTTTC CCGGGATGAC GTACACGTTG 
CGGGTGTATT CCCGGGACAA CGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT 
ACAGCTTACA CCAATAACCA GGCAGACATC GCCACCCAGG GCTGGTTCAT CGGGCTCATG 
TGTGCCATTG CCCTTCTGGT GCTGATCCTT CTCATCGTCT GCTTCATCAA GAGGAGTCGA 
GGTGGCAAGT ACCCAGTGCG GGAAAAGAAG GATGTCCCCT TGGGTCCTGA AGACCCCAAA 
GAAGAAGATG GCTCATTTGA CTACAGTGAT GAGGACAACA AGCCCCTGCA GGGCAGCCAG 
ACATCTCTGG ATGGCACCAT CAAGCAGCAG GAGAGCGATG ACAGCCTGGT GGACTATGGC 
GAAGGCGGCG AGGGCCAGTT CAATGAAGAT GGCTCCTTTA TTGGCCAGTA CACTGTCAAA 
AAGGACAAGG AGGAAACGGA GGGCAATGAG AGCTCAGAGG CCACATCACC AGTCAATGCC 
ATCTATTCCC TTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110377.1
CDS	51..4388
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_030254517.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254517.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG GGCGACCAGA CCGACCCAGG GACCTGGAGC TCACTGACCT GGCTGAGAGG 
AGTGTGAGGC TGACCTGGAT CCCAGGGGAT GACAACAACA GCCCTATCAC AGACTACGTC 
GTTCAGTTTG AAGAGGACCA GTTCCAACCA GGGGTGTGGC ATGACCACTC CAGGTTCCCA 
GGCAGCGTCA ACTCAGCCGT CCTCCATCTG TCCCCATATG TCAACTACCA ATTCAGAGTC 
ATCGCTGTCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTTC CATCCGAGCG GTACCGAACC 
AGTGGGGCAC CCCCTGAATC TAATCCCAGT GATGTGAAGG GCGAAGGGAC AAGAAAGAAC 
AATATGGAGA TCACGTGGAC GCCTATGAAT GCTACCTCTG CCTTTGGCCC CAACCTACGC 
TACATTGTCA AGTGGCGACG GAGAGAGACC CGAGAGACTT GGAACAATGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACGCCT GTCTACGTTC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTTGGGAA AGGCCCCGAG CCTGACACCA TCATTGGGTA CTCCGGAGAA 
GATTATCCCA GGGCTGCGCC CACTGAAGTT AAAATCCGAG TCCTGAACAG CACAGCCATC 
AGCCTTCAGT GGAACCGAGT CTACTCTGAC ACGGTCCAGG GCCAGCTCAG AGAGTATCGA 
GCTTACTACT GGAGGGAAAG CAGTTTGCTG AAGAACCTGT GGGTGTCTCA GAAGAGACAG 
CAGGCCAGCT TCCCTGGTGA CCGTCCCCGG GGCGTGGTGG CCCGCCTGTT CCCCTACAGT 
AACTACAAGC TGGAGATGGT GGTGGTCAAT GGGAGAGGTG ACGGGCCTCG AAGTGAAACC 
AAGGAATTCA CCACCCCAGA AGGAGTACCC AGTGCCCCCA GGCGGTTCAG AGTCCGACAG 
CCCAACCTGG AGACCATCAA CCTGGAGTGG GACCACCCAG AGCACCCCAA CGGAATCCTG 
ATTGGATACA TCCTCAGATA CGTGCCCTTT AATGGAACCA AACTGGGAAA GCAGATGGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC TCTGTGCAGA GAGCAGACCC AGTGTCGCGT 
TACCGCTTCT CCCTCAGTGC CAGGACACAG GTGGGCTCTG GAGAAGCAGC CACAGAGGAG 
TCCCCAGCAC CTCCAAATGA AGCTACTCCA ACTGCAGCTC CTCCCACGTT GCCCCCGACT 
ACTGTGGGTA CCACAGGCCT TGTGAGCAGT ACTGATGCTA CTGCCCTTGC TGCCACCAGT 
GAAGCCACAA CAGTTCCCAT CATTCCAACC GTCGTACCTA CCACCGTCGC CACCACCATT 
GCCACAACTA CTACAACCAC TGCCGCCACC ACCACCACCA CCACTACGGA GAGCCCTCCC 
ACTACCACTG CTGGGACTAA GATTCACGAA ACCGCCCCCG ACGAGCAGTC CATTTGGAAC 
GTCACAGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAAGCACAA TTTCAGGCCT 
GGAACTGACT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCTGTT 
AAGGCCCAGG CCCAGCCTAT ACAGCTGACA GACCTCTTTC CCGGGATGAC GTACACGTTG 
CGGGTGTATT CCCGGGACAA CGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT 
ACAGCTTACA CCAATAACCA GGCAGACATC GCCACCCAGG GCTGGTTCAT CGGGCTCATG 
TGTGCCATTG CCCTTCTGGT GCTGATCCTT CTCATCGTCT GCTTCATCAA GAGGAGTCGA 
GGTGGCAAGT ACCCAGTGCG GGAAAAGAAG GATGTCCCCT TGGGTCCTGA AGACCCCAAA 
GAAGAAGATG GCTCATTTGA CTACAGTGAT GAGGACAACA AGCCCCTGCA GGGCAGCCAG 
ACATCTCTGG ATGGCACCAT CAAGCAGCAG GAGAGCGATG ACAGCCTGGT GGACTATGGC 
GAAGGCGGCG AGGGCCAGTT CAATGAAGAT GGCTCCTTTA TTGGCCAGTA CACTGTCAAA 
AAGGACAAGG AGGAAACGGA GGGCAATGAG AGCTCAGAGG CCACATCACC AGTCAATGCC 
ATCTATTCCC TTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_030254521.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110381.1 Mus musculus neurofascin (Nfasc), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu85169
Clone ID Related Accession (Same CDS sequence)	XM_030254521.1
Accession Version	XM_030254521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCAGA AAGAACGCCC 
AGCTTCATGT ATCCCCAGGG CACATCGAGC AGTCAGATGG TTCTCCGTGG CATGGACCTG 
CTGCTGGAAT GCATTGCCTC TGGCGTCCCA ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTAACAA GGCCAAGTTC GAGAACTTTA ATAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCAT ACTGGCTGGA TGAGCCCAAG 
AACCTGATCC TGGCTCCTGG GGAAGATGGG AGGCTGGTAT GCCGAGCCAA TGGGAACCCG 
AAGCCGACCG TGCAGTGGAT GGTGAATGGA GAGCCTTTAC AATCGGCACC ACCCAATCCC 
AACCGTGAGG TAGCTGGAGA CACTATCATC TTCCGGGATA CTCAGATCAG CAGCAGGGCA 
GTGTACCAAT GTAATACATC CAATGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTGTTAGATG TACCCCCTCG GATGCTGTCT GCCCGCAACC AGCTCATCAG GGTGATCCTT 
TATAACCGGA CACGGCTGGA CTGTCCGTTC TTTGGGTCTC CCATCCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGCGGTAACT ACCACGTCTA CGAAAACGGC 
AGTCTAGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GCAAAGCCGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CCGAGGACCA GGTGGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAACATG ACCCCTCCTT GAAGCTCACA GTCTCCTGGC TGAAGGACGA TGAGCCACTC 
TACATTGGAA ACAGGATGAA GAAGGAAGAT GACTCCCTGA CGATCTTCGG AGTGGCAGAG 
CGGGACCAGG GCAGTTACAC GTGTATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGG GCGACCAGAC CGACCCAGGG ACCTGGAGCT CACTGACCTG 
GCTGAGAGGA GTGTGAGGCT GACCTGGATC CCAGGGGATG ACAACAACAG CCCTATCACA 
GACTACGTCG TTCAGTTTGA AGAGGACCAG TTCCAACCAG GGGTGTGGCA TGACCACTCC 
AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC CTCCATCTGT CCCCATATGT CAACTACCAA 
TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG AGCAGCCACC CCAGCCTTCC ATCCGAGCGG 
TACCGAACCA GTGGGGCACC CCCTGAATCT AATCCCAGTG ATGTGAAGGG CGAAGGGACA 
AGAAAGAACA ATATGGAGAT CACGTGGACG CCTATGAATG CTACCTCTGC CTTTGGCCCC 
AACCTACGCT ACATTGTCAA GTGGCGACGG AGAGAGACCC GAGAGACTTG GAACAATGTC 
ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG CAGACGCCTG TCTACGTTCC CTATGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAA GGCCCCGAGC CTGACACCAT CATTGGGTAC 
TCCGGAGAAG ATTATCCCAG GGCTGCGCCC ACTGAAGTTA AAATCCGAGT CCTGAACAGC 
ACAGCCATCA GCCTTCAGTG GAACCGAGTC TACTCTGACA CGGTCCAGGG CCAGCTCAGA 
GAGTATCGAG CTTACTACTG GAGGGAAAGC AGTTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGACAGC AGGCCAGCTT CCCTGGTGAC CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTG GTGGTCAATG GGAGAGGTGA CGGGCCTCGA 
AGTGAAACCA AGGAATTCAC CACCCCAGAA GGAGTACCCA GTGCCCCCAG GCGGTTCAGA 
GTCCGACAGC CCAACCTGGA GACCATCAAC CTGGAGTGGG ACCACCCAGA GCACCCCAAC 
GGAATCCTGA TTGGATACAT CCTCAGATAC GTGCCCTTTA ATGGAACCAA ACTGGGAAAG 
CAGATGGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCT CTGTGCAGAG AGCAGACCCA 
GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC AGGACACAGG TGGGCTCTGG AGAAGCAGCC 
ACAGAGGAGT CCCCAGCACC TCCAAATGAA GCTACTCCAA CTGCAGCTCC TCCCACGTTG 
CCCCCGACTA CTGTGGGTAC CACAGGCCTT GTGAGCAGTA CTGATGCTAC TGCCCTTGCT 
GCCACCAGTG AAGCCACAAC AGTTCCCATC ATTCCAACCG TCGTACCTAC CACCGTCGCC 
ACCACCATTG CCACAACTAC TACAACCACT GCCGCCACCA CCACCACCAC CACTACGGAG 
AGCCCTCCCA CTACCACTGC TGGGACTAAG ATTCACGAAA CCGCCCCCGA CGAGCAGTCC 
ATTTGGAACG TCACAGTGCT CCCCAACAGT AAATGGGCCA ACATCACCTG GAAGCACAAT 
TTCAGGCCTG GAACTGACTT TGTGGTTGAG TACATCGACA GCAACCATAC GAAAAAAACT 
GTCCCTGTTA AGGCCCAGGC CCAGCCTATA CAGCTGACAG ACCTCTTTCC CGGGATGACG 
TACACGTTGC GGGTGTATTC CCGGGACAAC GAGGGCATCA GCAGTACCGT CATCACCTTT 
ATGACCAGTA CAGCTTACAC CAATAACCAG GCAGACATCG CCACCCAGGG CTGGTTCATC 
GGGCTCATGT GTGCCATTGC CCTTCTGGTG CTGATCCTTC TCATCGTCTG CTTCATCAAG 
AGGAGTCGAG GTGGCAAGTA CCCAGTGCGG GAAAAGAAGG ATGTCCCCTT GGGTCCTGAA 
GACCCCAAAG AAGAAGATGG CTCATTTGAC TACAGTGATG AGGACAACAA GCCCCTGCAG 
GGCAGCCAGA CATCTCTGGA TGGCACCATC AAGCAGCAGG AGAGCGATGA CAGCCTGGTG 
GACTATGGCG AAGGCGGCGA GGGCCAGTTC AATGAAGATG GCTCCTTTAT TGGCCAGTAC 
ACTGTCAAAA AGGACAAGGA GGAAACGGAG GGCAATGAGA GCTCAGAGGC CACATCACCA 
GTCAATGCCA TCTATTCCCT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110381.1
CDS	51..4337
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_030254521.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254521.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCAGA AAGAACGCCC 
AGCTTCATGT ATCCCCAGGG CACATCGAGC AGTCAGATGG TTCTCCGTGG CATGGACCTG 
CTGCTGGAAT GCATTGCCTC TGGCGTCCCA ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTAACAA GGCCAAGTTC GAGAACTTTA ATAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCAT ACTGGCTGGA TGAGCCCAAG 
AACCTGATCC TGGCTCCTGG GGAAGATGGG AGGCTGGTAT GCCGAGCCAA TGGGAACCCG 
AAGCCGACCG TGCAGTGGAT GGTGAATGGA GAGCCTTTAC AATCGGCACC ACCCAATCCC 
AACCGTGAGG TAGCTGGAGA CACTATCATC TTCCGGGATA CTCAGATCAG CAGCAGGGCA 
GTGTACCAAT GTAATACATC CAATGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTGTTAGATG TACCCCCTCG GATGCTGTCT GCCCGCAACC AGCTCATCAG GGTGATCCTT 
TATAACCGGA CACGGCTGGA CTGTCCGTTC TTTGGGTCTC CCATCCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGCGGTAACT ACCACGTCTA CGAAAACGGC 
AGTCTAGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GCAAAGCCGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CCGAGGACCA GGTGGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAACATG ACCCCTCCTT GAAGCTCACA GTCTCCTGGC TGAAGGACGA TGAGCCACTC 
TACATTGGAA ACAGGATGAA GAAGGAAGAT GACTCCCTGA CGATCTTCGG AGTGGCAGAG 
CGGGACCAGG GCAGTTACAC GTGTATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGG GCGACCAGAC CGACCCAGGG ACCTGGAGCT CACTGACCTG 
GCTGAGAGGA GTGTGAGGCT GACCTGGATC CCAGGGGATG ACAACAACAG CCCTATCACA 
GACTACGTCG TTCAGTTTGA AGAGGACCAG TTCCAACCAG GGGTGTGGCA TGACCACTCC 
AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC CTCCATCTGT CCCCATATGT CAACTACCAA 
TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG AGCAGCCACC CCAGCCTTCC ATCCGAGCGG 
TACCGAACCA GTGGGGCACC CCCTGAATCT AATCCCAGTG ATGTGAAGGG CGAAGGGACA 
AGAAAGAACA ATATGGAGAT CACGTGGACG CCTATGAATG CTACCTCTGC CTTTGGCCCC 
AACCTACGCT ACATTGTCAA GTGGCGACGG AGAGAGACCC GAGAGACTTG GAACAATGTC 
ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG CAGACGCCTG TCTACGTTCC CTATGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAA GGCCCCGAGC CTGACACCAT CATTGGGTAC 
TCCGGAGAAG ATTATCCCAG GGCTGCGCCC ACTGAAGTTA AAATCCGAGT CCTGAACAGC 
ACAGCCATCA GCCTTCAGTG GAACCGAGTC TACTCTGACA CGGTCCAGGG CCAGCTCAGA 
GAGTATCGAG CTTACTACTG GAGGGAAAGC AGTTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGACAGC AGGCCAGCTT CCCTGGTGAC CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTG GTGGTCAATG GGAGAGGTGA CGGGCCTCGA 
AGTGAAACCA AGGAATTCAC CACCCCAGAA GGAGTACCCA GTGCCCCCAG GCGGTTCAGA 
GTCCGACAGC CCAACCTGGA GACCATCAAC CTGGAGTGGG ACCACCCAGA GCACCCCAAC 
GGAATCCTGA TTGGATACAT CCTCAGATAC GTGCCCTTTA ATGGAACCAA ACTGGGAAAG 
CAGATGGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCT CTGTGCAGAG AGCAGACCCA 
GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC AGGACACAGG TGGGCTCTGG AGAAGCAGCC 
ACAGAGGAGT CCCCAGCACC TCCAAATGAA GCTACTCCAA CTGCAGCTCC TCCCACGTTG 
CCCCCGACTA CTGTGGGTAC CACAGGCCTT GTGAGCAGTA CTGATGCTAC TGCCCTTGCT 
GCCACCAGTG AAGCCACAAC AGTTCCCATC ATTCCAACCG TCGTACCTAC CACCGTCGCC 
ACCACCATTG CCACAACTAC TACAACCACT GCCGCCACCA CCACCACCAC CACTACGGAG 
AGCCCTCCCA CTACCACTGC TGGGACTAAG ATTCACGAAA CCGCCCCCGA CGAGCAGTCC 
ATTTGGAACG TCACAGTGCT CCCCAACAGT AAATGGGCCA ACATCACCTG GAAGCACAAT 
TTCAGGCCTG GAACTGACTT TGTGGTTGAG TACATCGACA GCAACCATAC GAAAAAAACT 
GTCCCTGTTA AGGCCCAGGC CCAGCCTATA CAGCTGACAG ACCTCTTTCC CGGGATGACG 
TACACGTTGC GGGTGTATTC CCGGGACAAC GAGGGCATCA GCAGTACCGT CATCACCTTT 
ATGACCAGTA CAGCTTACAC CAATAACCAG GCAGACATCG CCACCCAGGG CTGGTTCATC 
GGGCTCATGT GTGCCATTGC CCTTCTGGTG CTGATCCTTC TCATCGTCTG CTTCATCAAG 
AGGAGTCGAG GTGGCAAGTA CCCAGTGCGG GAAAAGAAGG ATGTCCCCTT GGGTCCTGAA 
GACCCCAAAG AAGAAGATGG CTCATTTGAC TACAGTGATG AGGACAACAA GCCCCTGCAG 
GGCAGCCAGA CATCTCTGGA TGGCACCATC AAGCAGCAGG AGAGCGATGA CAGCCTGGTG 
GACTATGGCG AAGGCGGCGA GGGCCAGTTC AATGAAGATG GCTCCTTTAT TGGCCAGTAC 
ACTGTCAAAA AGGACAAGGA GGAAACGGAG GGCAATGAGA GCTCAGAGGC CACATCACCA 
GTCAATGCCA TCTATTCCCT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_030254536.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110396.1 Mus musculus neurofascin (Nfasc), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OMu85170
Clone ID Related Accession (Same CDS sequence)	XM_030254536.1
Accession Version	XM_030254536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGGG CGACCAGACC GACCCAGGGA CCTGGAGCTC 
ACTGACCTGG CTGAGAGGAG TGTGAGGCTG ACCTGGATCC CAGGGGATGA CAACAACAGC 
CCTATCACAG ACTACGTCGT TCAGTTTGAA GAGGACCAGT TCCAACCAGG GGTGTGGCAT 
GACCACTCCA GGTTCCCAGG CAGCGTCAAC TCAGCCGTCC TCCATCTGTC CCCATATGTC 
AACTACCAAT TCAGAGTCAT CGCTGTCAAC GAGGTTGGGA GCAGCCACCC CAGCCTTCCA 
TCCGAGCGGT ACCGAACCAG TGGGGCACCC CCTGAATCTA ATCCCAGTGA TGTGAAGGGC 
GAAGGGACAA GAAAGAACAA TATGGAGATC ACGTGGACGC CTATGAATGC TACCTCTGCC 
TTTGGCCCCA ACCTACGCTA CATTGTCAAG TGGCGACGGA GAGAGACCCG AGAGACTTGG 
AACAATGTCA CAGTGTGGGG CTCTCGCTAC GTGGTGGGGC AGACGCCTGT CTACGTTCCC 
TATGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAAG GCCCCGAGCC TGACACCATC 
ATTGGGTACT CCGGAGAAGA TTATCCCAGG GCTGCGCCCA CTGAAGTTAA AATCCGAGTC 
CTGAACAGCA CAGCCATCAG CCTTCAGTGG AACCGAGTCT ACTCTGACAC GGTCCAGGGC 
CAGCTCAGAG AGTATCGAGC TTACTACTGG AGGGAAAGCA GTTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGACAGCA GGCCAGCTTC CCTGGTGACC GTCCCCGGGG CGTGGTGGCC 
CGCCTGTTCC CCTACAGTAA CTACAAGCTG GAGATGGTGG TGGTCAATGG GAGAGGTGAC 
GGGCCTCGAA GTGAAACCAA GGAATTCACC ACCCCAGAAG GAGTACCCAG TGCCCCCAGG 
CGGTTCAGAG TCCGACAGCC CAACCTGGAG ACCATCAACC TGGAGTGGGA CCACCCAGAG 
CACCCCAACG GAATCCTGAT TGGATACATC CTCAGATACG TGCCCTTTAA TGGAACCAAA 
CTGGGAAAGC AGATGGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCTC TGTGCAGAGA 
GCAGACCCAG TGTCGCGTTA CCGCTTCTCC CTCAGTGCCA GGACACAGGT GGGCTCTGGA 
GAAGCAGCCA CAGAGGAGTC CCCAGCACCT CCAAATGAAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110396.1
CDS	53..3886
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_030254536.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254536.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGGG CGACCAGACC GACCCAGGGA CCTGGAGCTC 
ACTGACCTGG CTGAGAGGAG TGTGAGGCTG ACCTGGATCC CAGGGGATGA CAACAACAGC 
CCTATCACAG ACTACGTCGT TCAGTTTGAA GAGGACCAGT TCCAACCAGG GGTGTGGCAT 
GACCACTCCA GGTTCCCAGG CAGCGTCAAC TCAGCCGTCC TCCATCTGTC CCCATATGTC 
AACTACCAAT TCAGAGTCAT CGCTGTCAAC GAGGTTGGGA GCAGCCACCC CAGCCTTCCA 
TCCGAGCGGT ACCGAACCAG TGGGGCACCC CCTGAATCTA ATCCCAGTGA TGTGAAGGGC 
GAAGGGACAA GAAAGAACAA TATGGAGATC ACGTGGACGC CTATGAATGC TACCTCTGCC 
TTTGGCCCCA ACCTACGCTA CATTGTCAAG TGGCGACGGA GAGAGACCCG AGAGACTTGG 
AACAATGTCA CAGTGTGGGG CTCTCGCTAC GTGGTGGGGC AGACGCCTGT CTACGTTCCC 
TATGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAAG GCCCCGAGCC TGACACCATC 
ATTGGGTACT CCGGAGAAGA TTATCCCAGG GCTGCGCCCA CTGAAGTTAA AATCCGAGTC 
CTGAACAGCA CAGCCATCAG CCTTCAGTGG AACCGAGTCT ACTCTGACAC GGTCCAGGGC 
CAGCTCAGAG AGTATCGAGC TTACTACTGG AGGGAAAGCA GTTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGACAGCA GGCCAGCTTC CCTGGTGACC GTCCCCGGGG CGTGGTGGCC 
CGCCTGTTCC CCTACAGTAA CTACAAGCTG GAGATGGTGG TGGTCAATGG GAGAGGTGAC 
GGGCCTCGAA GTGAAACCAA GGAATTCACC ACCCCAGAAG GAGTACCCAG TGCCCCCAGG 
CGGTTCAGAG TCCGACAGCC CAACCTGGAG ACCATCAACC TGGAGTGGGA CCACCCAGAG 
CACCCCAACG GAATCCTGAT TGGATACATC CTCAGATACG TGCCCTTTAA TGGAACCAAA 
CTGGGAAAGC AGATGGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCTC TGTGCAGAGA 
GCAGACCCAG TGTCGCGTTA CCGCTTCTCC CTCAGTGCCA GGACACAGGT GGGCTCTGGA 
GAAGCAGCCA CAGAGGAGTC CCCAGCACCT CCAAATGAAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_030254549.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110409.1 Mus musculus neurofascin (Nfasc), transcript variant X26, mRNA.

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CloneID	OMu85171
Clone ID Related Accession (Same CDS sequence)	XM_030254549.1
Accession Version	XM_030254549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACTATCA CCAAGCAGTC CGTGAAGGAC CACATCGTGG ACCCTCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG CAACCCCGCC CCCAGTTTTC ACTGGACTCG CAACAGCAGA 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGATCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAATACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TTGGCACTGC ACTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTGACACTA 
CAGTGCAACC CCCCACCCGG CCTCCCGTCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ACGCGCGCTT TCACTTCACC 
CACACCATTC AGCAGAAGAA CCCCTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACGC CCAGCTTCAT GTATCCCCAG GGCACATCGA GCAGTCAGAT GGTTCTCCGT 
GGCATGGACC TGCTGCTGGA ATGCATTGCC TCTGGCGTCC CAACACCAGA CATTGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTAAC AAGGCCAAGT TCGAGAACTT TAATAAGGCT 
CTGCGCATCA CCAATGTCTC TGAAGAGGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC ATACTGGCTG 
GATGAGCCCA AGAACCTGAT CCTGGCTCCT GGGGAAGATG GGAGGCTGGT ATGCCGAGCC 
AATGGGAACC CGAAGCCGAC CGTGCAGTGG ATGGTGAATG GAGAGCCTTT ACAATCGGCA 
CCACCCAATC CCAACCGTGA GGTAGCTGGA GACACTATCA TCTTCCGGGA TACTCAGATC 
AGCAGCAGGG CAGTGTACCA ATGTAATACA TCCAATGAAC ATGGCTACCT GCTGGCCAAT 
GCCTTCGTCA GCGTGTTAGA TGTACCCCCT CGGATGCTGT CTGCCCGCAA CCAGCTCATC 
AGGGTGATCC TTTATAACCG GACACGGCTG GACTGTCCGT TCTTTGGGTC TCCCATCCCA 
ACACTCCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGCGGTAA CTACCACGTC 
TACGAAAACG GCAGTCTAGA AATCAAGATG ATTCGCAAAG AGGACCAAGG CATCTACACC 
TGTGTGGCCA CCAACATCCT GGGCAAAGCC GAAAATCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACAGGAT GCCCGAGGAC CAGGTGGCCA AGAGGGGCAC CACGGTGCAG 
CTGGAGTGCC GCGTGAAACA TGACCCCTCC TTGAAGCTCA CAGTCTCCTG GCTGAAGGAC 
GATGAGCCAC TCTACATTGG AAACAGGATG AAGAAGGAAG ATGACTCCCT GACGATCTTC 
GGAGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTATGG CCAGCACCGA GCTGGACCAG 
GACCTGGCAA AGGCCTACCT CACTGTTCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTAC CCAAAGGGCG ACCAGACCGA CCCAGGGACC TGGAGCTCAC TGACCTGGCT 
GAGAGGAGTG TGAGGCTGAC CTGGATCCCA GGGGATGACA ACAACAGCCC TATCACAGAC 
TACGTCGTTC AGTTTGAAGA GGACCAGTTC CAACCAGGGG TGTGGCATGA CCACTCCAGG 
TTCCCAGGCA GCGTCAACTC AGCCGTCCTC CATCTGTCCC CATATGTCAA CTACCAATTC 
AGAGTCATCG CTGTCAACGA GGTTGGGAGC AGCCACCCCA GCCTTCCATC CGAGCGGTAC 
CGAACCAGTG GGGCACCCCC TGAATCTAAT CCCAGTGATG TGAAGGGCGA AGGGACAAGA 
AAGAACAATA TGGAGATCAC GTGGACGCCT ATGAATGCTA CCTCTGCCTT TGGCCCCAAC 
CTACGCTACA TTGTCAAGTG GCGACGGAGA GAGACCCGAG AGACTTGGAA CAATGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACGCCTGTCT ACGTTCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT TGGGAAAGGC CCCGAGCCTG ACACCATCAT TGGGTACTCC 
GGAGAAGATT ATCCCAGGGC TGCGCCCACT GAAGTTAAAA TCCGAGTCCT GAACAGCACA 
GCCATCAGCC TTCAGTGGAA CCGAGTCTAC TCTGACACGG TCCAGGGCCA GCTCAGAGAG 
TATCGAGCTT ACTACTGGAG GGAAAGCAGT TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG 
AGACAGCAGG CCAGCTTCCC TGGTGACCGT CCCCGGGGCG TGGTGGCCCG CCTGTTCCCC 
TACAGTAACT ACAAGCTGGA GATGGTGGTG GTCAATGGGA GAGGTGACGG GCCTCGAAGT 
GAAACCAAGG AATTCACCAC CCCAGAAGGA GTACCCAGTG CCCCCAGGCG GTTCAGAGTC 
CGACAGCCCA ACCTGGAGAC CATCAACCTG GAGTGGGACC ACCCAGAGCA CCCCAACGGA 
ATCCTGATTG GATACATCCT CAGATACGTG CCCTTTAATG GAACCAAACT GGGAAAGCAG 
ATGGTGGAAA ACTTCTCTCC CAATCAGACC AAGTTCTCTG TGCAGAGAGC AGACCCAGTG 
TCGCGTTACC GCTTCTCCCT CAGTGCCAGG ACACAGGTGG GCTCTGGAGA AGCAGCCACA 
GAGGAGTCCC CAGCACCTCC AAATGAAGCT ACTCCAACTG CAGCTCCTCC CACGTTGCCC 
CCGACTACTG TGGGTACCAC AGGCCTTGTG AGCAGTACTG ATGCTACTGC CCTTGCTGCC 
ACCAGTGAAG CCACAACAGT TCCCATCATT CCAACCGTCG TACCTACCAC CGTCGCCACC 
ACCATTGCCA CAACTACTAC AACCACTGCC GCCACCACCA CCACCACCAC TACGGAGAGC 
CCTCCCACTA CCACTGCTGG GACTAAGATT CACGAAACCG CCCCCGACGA GCAGTCCATT 
TGGAACGTCA CAGTGCTCCC CAACAGTAAA TGGGCCAACA TCACCTGGAA GCACAATTTC 
AGGCCTGGAA CTGACTTTGT GGTTGAGTAC ATCGACAGCA ACCATACGAA AAAAACTGTC 
CCTGTTAAGG CCCAGGCCCA GCCTATACAG CTGACAGACC TCTTTCCCGG GATGACGTAC 
ACGTTGCGGG TGTATTCCCG GGACAACGAG GGCATCAGCA GTACCGTCAT CACCTTTATG 
ACCAGTACAG CTTACACCAA TAACCAGGCA GACATCGCCA CCCAGGGCTG GTTCATCGGG 
CTCATGTGTG CCATTGCCCT TCTGGTGCTG ATCCTTCTCA TCGTCTGCTT CATCAAGAGG 
AGTCGAGGTG GCAAGTACCC AGTGCGGGAA AAGAAGGATG TCCCCTTGGG TCCTGAAGAC 
CCCAAAGAAG AAGATGGCTC ATTTGACTAC AGTGATGAGG ACAACAAGCC CCTGCAGGGC 
AGCCAGACAT CTCTGGATGG CACCATCAAG CAGCAGGAGA GCGATGACAG CCTGGTGGAC 
TATGGCGAAG GCGGCGAGGG CCAGTTCAAT GAAGATGGCT CCTTTATTGG CCAGTACACT 
GTCAAAAAGG ACAAGGAGGA AACGGAGGGC AATGAGAGCT CAGAGGCCAC ATCACCAGTC 
AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110409.1
CDS	175..4218
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_030254549.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254549.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACTATCA CCAAGCAGTC CGTGAAGGAC CACATCGTGG ACCCTCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG CAACCCCGCC CCCAGTTTTC ACTGGACTCG CAACAGCAGA 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGATCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAATACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TTGGCACTGC ACTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTGACACTA 
CAGTGCAACC CCCCACCCGG CCTCCCGTCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ACGCGCGCTT TCACTTCACC 
CACACCATTC AGCAGAAGAA CCCCTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACGC CCAGCTTCAT GTATCCCCAG GGCACATCGA GCAGTCAGAT GGTTCTCCGT 
GGCATGGACC TGCTGCTGGA ATGCATTGCC TCTGGCGTCC CAACACCAGA CATTGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTAAC AAGGCCAAGT TCGAGAACTT TAATAAGGCT 
CTGCGCATCA CCAATGTCTC TGAAGAGGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC ATACTGGCTG 
GATGAGCCCA AGAACCTGAT CCTGGCTCCT GGGGAAGATG GGAGGCTGGT ATGCCGAGCC 
AATGGGAACC CGAAGCCGAC CGTGCAGTGG ATGGTGAATG GAGAGCCTTT ACAATCGGCA 
CCACCCAATC CCAACCGTGA GGTAGCTGGA GACACTATCA TCTTCCGGGA TACTCAGATC 
AGCAGCAGGG CAGTGTACCA ATGTAATACA TCCAATGAAC ATGGCTACCT GCTGGCCAAT 
GCCTTCGTCA GCGTGTTAGA TGTACCCCCT CGGATGCTGT CTGCCCGCAA CCAGCTCATC 
AGGGTGATCC TTTATAACCG GACACGGCTG GACTGTCCGT TCTTTGGGTC TCCCATCCCA 
ACACTCCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGCGGTAA CTACCACGTC 
TACGAAAACG GCAGTCTAGA AATCAAGATG ATTCGCAAAG AGGACCAAGG CATCTACACC 
TGTGTGGCCA CCAACATCCT GGGCAAAGCC GAAAATCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACAGGAT GCCCGAGGAC CAGGTGGCCA AGAGGGGCAC CACGGTGCAG 
CTGGAGTGCC GCGTGAAACA TGACCCCTCC TTGAAGCTCA CAGTCTCCTG GCTGAAGGAC 
GATGAGCCAC TCTACATTGG AAACAGGATG AAGAAGGAAG ATGACTCCCT GACGATCTTC 
GGAGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTATGG CCAGCACCGA GCTGGACCAG 
GACCTGGCAA AGGCCTACCT CACTGTTCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTAC CCAAAGGGCG ACCAGACCGA CCCAGGGACC TGGAGCTCAC TGACCTGGCT 
GAGAGGAGTG TGAGGCTGAC CTGGATCCCA GGGGATGACA ACAACAGCCC TATCACAGAC 
TACGTCGTTC AGTTTGAAGA GGACCAGTTC CAACCAGGGG TGTGGCATGA CCACTCCAGG 
TTCCCAGGCA GCGTCAACTC AGCCGTCCTC CATCTGTCCC CATATGTCAA CTACCAATTC 
AGAGTCATCG CTGTCAACGA GGTTGGGAGC AGCCACCCCA GCCTTCCATC CGAGCGGTAC 
CGAACCAGTG GGGCACCCCC TGAATCTAAT CCCAGTGATG TGAAGGGCGA AGGGACAAGA 
AAGAACAATA TGGAGATCAC GTGGACGCCT ATGAATGCTA CCTCTGCCTT TGGCCCCAAC 
CTACGCTACA TTGTCAAGTG GCGACGGAGA GAGACCCGAG AGACTTGGAA CAATGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACGCCTGTCT ACGTTCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT TGGGAAAGGC CCCGAGCCTG ACACCATCAT TGGGTACTCC 
GGAGAAGATT ATCCCAGGGC TGCGCCCACT GAAGTTAAAA TCCGAGTCCT GAACAGCACA 
GCCATCAGCC TTCAGTGGAA CCGAGTCTAC TCTGACACGG TCCAGGGCCA GCTCAGAGAG 
TATCGAGCTT ACTACTGGAG GGAAAGCAGT TTGCTGAAGA ACCTGTGGGT GTCTCAGAAG 
AGACAGCAGG CCAGCTTCCC TGGTGACCGT CCCCGGGGCG TGGTGGCCCG CCTGTTCCCC 
TACAGTAACT ACAAGCTGGA GATGGTGGTG GTCAATGGGA GAGGTGACGG GCCTCGAAGT 
GAAACCAAGG AATTCACCAC CCCAGAAGGA GTACCCAGTG CCCCCAGGCG GTTCAGAGTC 
CGACAGCCCA ACCTGGAGAC CATCAACCTG GAGTGGGACC ACCCAGAGCA CCCCAACGGA 
ATCCTGATTG GATACATCCT CAGATACGTG CCCTTTAATG GAACCAAACT GGGAAAGCAG 
ATGGTGGAAA ACTTCTCTCC CAATCAGACC AAGTTCTCTG TGCAGAGAGC AGACCCAGTG 
TCGCGTTACC GCTTCTCCCT CAGTGCCAGG ACACAGGTGG GCTCTGGAGA AGCAGCCACA 
GAGGAGTCCC CAGCACCTCC AAATGAAGCT ACTCCAACTG CAGCTCCTCC CACGTTGCCC 
CCGACTACTG TGGGTACCAC AGGCCTTGTG AGCAGTACTG ATGCTACTGC CCTTGCTGCC 
ACCAGTGAAG CCACAACAGT TCCCATCATT CCAACCGTCG TACCTACCAC CGTCGCCACC 
ACCATTGCCA CAACTACTAC AACCACTGCC GCCACCACCA CCACCACCAC TACGGAGAGC 
CCTCCCACTA CCACTGCTGG GACTAAGATT CACGAAACCG CCCCCGACGA GCAGTCCATT 
TGGAACGTCA CAGTGCTCCC CAACAGTAAA TGGGCCAACA TCACCTGGAA GCACAATTTC 
AGGCCTGGAA CTGACTTTGT GGTTGAGTAC ATCGACAGCA ACCATACGAA AAAAACTGTC 
CCTGTTAAGG CCCAGGCCCA GCCTATACAG CTGACAGACC TCTTTCCCGG GATGACGTAC 
ACGTTGCGGG TGTATTCCCG GGACAACGAG GGCATCAGCA GTACCGTCAT CACCTTTATG 
ACCAGTACAG CTTACACCAA TAACCAGGCA GACATCGCCA CCCAGGGCTG GTTCATCGGG 
CTCATGTGTG CCATTGCCCT TCTGGTGCTG ATCCTTCTCA TCGTCTGCTT CATCAAGAGG 
AGTCGAGGTG GCAAGTACCC AGTGCGGGAA AAGAAGGATG TCCCCTTGGG TCCTGAAGAC 
CCCAAAGAAG AAGATGGCTC ATTTGACTAC AGTGATGAGG ACAACAAGCC CCTGCAGGGC 
AGCCAGACAT CTCTGGATGG CACCATCAAG CAGCAGGAGA GCGATGACAG CCTGGTGGAC 
TATGGCGAAG GCGGCGAGGG CCAGTTCAAT GAAGATGGCT CCTTTATTGG CCAGTACACT 
GTCAAAAAGG ACAAGGAGGA AACGGAGGGC AATGAGAGCT CAGAGGCCAC ATCACCAGTC 
AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_030254551.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110411.1 Mus musculus neurofascin (Nfasc), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OMu85172
Clone ID Related Accession (Same CDS sequence)	XM_030254551.1
Accession Version	XM_030254551.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGGGCGAC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG 
AGGCTGACCT GGATCCCAGG GGATGACAAC AACAGCCCTA TCACAGACTA CGTCGTTCAG 
TTTGAAGAGG ACCAGTTCCA ACCAGGGGTG TGGCATGACC ACTCCAGGTT CCCAGGCAGC 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCA TATGTCAACT ACCAATTCAG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACCAGTGGG 
GCACCCCCTG AATCTAATCC CAGTGATGTG AAGGGCGAAG GGACAAGAAA GAACAATATG 
GAGATCACGT GGACGCCTAT GAATGCTACC TCTGCCTTTG GCCCCAACCT ACGCTACATT 
GTCAAGTGGC GACGGAGAGA GACCCGAGAG ACTTGGAACA ATGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC GCCTGTCTAC GTTCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC CGAGCCTGAC ACCATCATTG GGTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GAGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GTGACCGTCC CCGGGGCGTG GTGGCCCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTGGTGGT CAATGGGAGA GGTGACGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGGT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA GTGGGACCAC CCAGAGCACC CCAACGGAAT CCTGATTGGA 
TACATCCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCTGTG CAGAGAGCAG ACCCAGTGTC GCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC ACAGGTGGGC TCTGGAGAAG CAGCCACAGA GGAGTCCCCA 
GCACCTCCAA ATGAAGCTAC TCCAACTGCA GCTCCTCCCA CGTTGCCCCC GACTACTGTG 
GGTACCACAG GCCTTGTGAG CAGTACTGAT GCTACTGCCC TTGCTGCCAC CAGTGAAGCC 
ACAACAGTTC CCATCATTCC AACCGTCGTA CCTACCACCG TCGCCACCAC CATTGCCACA 
ACTACTACAA CCACTGCCGC CACCACCACC ACCACCACTA CGGAGAGCCC TCCCACTACC 
ACTGCTGGGA CTAAGATTCA CGAAACCGCC CCCGACGAGC AGTCCATTTG GAACGTCACA 
GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCAG GCCTGGAACT 
GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC TGTTAAGGCC 
CAGGCCCAGC CTATACAGCT GACAGACCTC TTTCCCGGGA TGACGTACAC GTTGCGGGTG 
TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT 
TACACCAATA ACCAGGCAGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGTGCC 
ATTGCCCTTC TGGTGCTGAT CCTTCTCATC GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC 
AAGTACCCAG TGCGGGAAAA GAAGGATGTC CCCTTGGGTC CTGAAGACCC CAAAGAAGAA 
GATGGCTCAT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCT 
CTGGATGGCA CCATCAAGCA GCAGGAGAGC GATGACAGCC TGGTGGACTA TGGCGAAGGC 
GGCGAGGGCC AGTTCAATGA AGATGGCTCC TTTATTGGCC AGTACACTGT CAAAAAGGAC 
AAGGAGGAAA CGGAGGGCAA TGAGAGCTCA GAGGCCACAT CACCAGTCAA TGCCATCTAT 
TCCCTTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110411.1
CDS	425..4456
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_030254551.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254551.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGGGCGAC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG 
AGGCTGACCT GGATCCCAGG GGATGACAAC AACAGCCCTA TCACAGACTA CGTCGTTCAG 
TTTGAAGAGG ACCAGTTCCA ACCAGGGGTG TGGCATGACC ACTCCAGGTT CCCAGGCAGC 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCA TATGTCAACT ACCAATTCAG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACCAGTGGG 
GCACCCCCTG AATCTAATCC CAGTGATGTG AAGGGCGAAG GGACAAGAAA GAACAATATG 
GAGATCACGT GGACGCCTAT GAATGCTACC TCTGCCTTTG GCCCCAACCT ACGCTACATT 
GTCAAGTGGC GACGGAGAGA GACCCGAGAG ACTTGGAACA ATGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC GCCTGTCTAC GTTCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC CGAGCCTGAC ACCATCATTG GGTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GAGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GTGACCGTCC CCGGGGCGTG GTGGCCCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTGGTGGT CAATGGGAGA GGTGACGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGGT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA GTGGGACCAC CCAGAGCACC CCAACGGAAT CCTGATTGGA 
TACATCCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCTGTG CAGAGAGCAG ACCCAGTGTC GCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC ACAGGTGGGC TCTGGAGAAG CAGCCACAGA GGAGTCCCCA 
GCACCTCCAA ATGAAGCTAC TCCAACTGCA GCTCCTCCCA CGTTGCCCCC GACTACTGTG 
GGTACCACAG GCCTTGTGAG CAGTACTGAT GCTACTGCCC TTGCTGCCAC CAGTGAAGCC 
ACAACAGTTC CCATCATTCC AACCGTCGTA CCTACCACCG TCGCCACCAC CATTGCCACA 
ACTACTACAA CCACTGCCGC CACCACCACC ACCACCACTA CGGAGAGCCC TCCCACTACC 
ACTGCTGGGA CTAAGATTCA CGAAACCGCC CCCGACGAGC AGTCCATTTG GAACGTCACA 
GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCAG GCCTGGAACT 
GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC TGTTAAGGCC 
CAGGCCCAGC CTATACAGCT GACAGACCTC TTTCCCGGGA TGACGTACAC GTTGCGGGTG 
TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT 
TACACCAATA ACCAGGCAGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGTGCC 
ATTGCCCTTC TGGTGCTGAT CCTTCTCATC GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC 
AAGTACCCAG TGCGGGAAAA GAAGGATGTC CCCTTGGGTC CTGAAGACCC CAAAGAAGAA 
GATGGCTCAT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCT 
CTGGATGGCA CCATCAAGCA GCAGGAGAGC GATGACAGCC TGGTGGACTA TGGCGAAGGC 
GGCGAGGGCC AGTTCAATGA AGATGGCTCC TTTATTGGCC AGTACACTGT CAAAAAGGAC 
AAGGAGGAAA CGGAGGGCAA TGAGAGCTCA GAGGCCACAT CACCAGTCAA TGCCATCTAT 
TCCCTTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_030254559.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110419.1 Mus musculus neurofascin (Nfasc), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OMu85173
Clone ID Related Accession (Same CDS sequence)	XM_030254559.1
Accession Version	XM_030254559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG GGCGACCAGA CCGACCCAGG GACCTGGAGC TCACTGACCT GGCTGAGAGG 
AGTGTGAGGC TGACCTGGAT CCCAGGGGAT GACAACAACA GCCCTATCAC AGACTACGTC 
GTTCAGTTTG AAGAGGACCA GTTCCAACCA GGGGTGTGGC ATGACCACTC CAGGTTCCCA 
GGCAGCGTCA ACTCAGCCGT CCTCCATCTG TCCCCATATG TCAACTACCA ATTCAGAGTC 
ATCGCTGTCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTTC CATCCGAGCG GTACCGAACC 
AGTGGGGCAC CCCCTGAATC TAATCCCAGT GATGTGAAGG GCGAAGGGAC AAGAAAGAAC 
AATATGGAGA TCACGTGGAC GCCTATGAAT GCTACCTCTG CCTTTGGCCC CAACCTACGC 
TACATTGTCA AGTGGCGACG GAGAGAGACC CGAGAGACTT GGAACAATGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACGCCT GTCTACGTTC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTTGGGAA AGGCCCCGAG CCTGACACCA TCATTGGGTA CTCCGGAGAA 
GATTTACCCA GTGCCCCCAG GCGGTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC 
CTGGAGTGGG ACCACCCAGA GCACCCCAAC GGAATCCTGA TTGGATACAT CCTCAGATAC 
GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCT CTGTGCAGAG AGCAGACCCA GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC 
AGGACACAGG TGGGCTCTGG AGAAGCAGCC ACAGAGGAGT CCCCAGCACC TCCAAATGAA 
GCTTACACCA ATAACCAGGC AGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT 
GCCATTGCCC TTCTGGTGCT GATCCTTCTC ATCGTCTGCT TCATCAAGAG GAGTCGAGGT 
GGCAAGTACC CAGTGCGGGA AAAGAAGGAT GTCCCCTTGG GTCCTGAAGA CCCCAAAGAA 
GAAGATGGCT CATTTGACTA CAGTGATGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA 
TCTCTGGATG GCACCATCAA GCAGCAGGAG AGCGATGACA GCCTGGTGGA CTATGGCGAA 
GGCGGCGAGG GCCAGTTCAA TGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAAAAAG 
GACAAGGAGG AAACGGAGGG CAATGAGAGC TCAGAGGCCA CATCACCAGT CAATGCCATC 
TATTCCCTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110419.1
CDS	50..3544
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_030254559.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254559.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG GGCGACCAGA CCGACCCAGG GACCTGGAGC TCACTGACCT GGCTGAGAGG 
AGTGTGAGGC TGACCTGGAT CCCAGGGGAT GACAACAACA GCCCTATCAC AGACTACGTC 
GTTCAGTTTG AAGAGGACCA GTTCCAACCA GGGGTGTGGC ATGACCACTC CAGGTTCCCA 
GGCAGCGTCA ACTCAGCCGT CCTCCATCTG TCCCCATATG TCAACTACCA ATTCAGAGTC 
ATCGCTGTCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTTC CATCCGAGCG GTACCGAACC 
AGTGGGGCAC CCCCTGAATC TAATCCCAGT GATGTGAAGG GCGAAGGGAC AAGAAAGAAC 
AATATGGAGA TCACGTGGAC GCCTATGAAT GCTACCTCTG CCTTTGGCCC CAACCTACGC 
TACATTGTCA AGTGGCGACG GAGAGAGACC CGAGAGACTT GGAACAATGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACGCCT GTCTACGTTC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTTGGGAA AGGCCCCGAG CCTGACACCA TCATTGGGTA CTCCGGAGAA 
GATTTACCCA GTGCCCCCAG GCGGTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC 
CTGGAGTGGG ACCACCCAGA GCACCCCAAC GGAATCCTGA TTGGATACAT CCTCAGATAC 
GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCT CTGTGCAGAG AGCAGACCCA GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC 
AGGACACAGG TGGGCTCTGG AGAAGCAGCC ACAGAGGAGT CCCCAGCACC TCCAAATGAA 
GCTTACACCA ATAACCAGGC AGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT 
GCCATTGCCC TTCTGGTGCT GATCCTTCTC ATCGTCTGCT TCATCAAGAG GAGTCGAGGT 
GGCAAGTACC CAGTGCGGGA AAAGAAGGAT GTCCCCTTGG GTCCTGAAGA CCCCAAAGAA 
GAAGATGGCT CATTTGACTA CAGTGATGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA 
TCTCTGGATG GCACCATCAA GCAGCAGGAG AGCGATGACA GCCTGGTGGA CTATGGCGAA 
GGCGGCGAGG GCCAGTTCAA TGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAAAAAG 
GACAAGGAGG AAACGGAGGG CAATGAGAGC TCAGAGGCCA CATCACCAGT CAATGCCATC 
TATTCCCTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_030254562.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110422.1 Mus musculus neurofascin (Nfasc), transcript variant X34, mRNA.

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CloneID	OMu85174
Clone ID Related Accession (Same CDS sequence)	XM_030254562.1
Accession Version	XM_030254562.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGGGCGAC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG 
AGGCTGACCT GGATCCCAGG GGATGACAAC AACAGCCCTA TCACAGACTA CGTCGTTCAG 
TTTGAAGAGG ACCAGTTCCA ACCAGGGGTG TGGCATGACC ACTCCAGGTT CCCAGGCAGC 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCA TATGTCAACT ACCAATTCAG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACCAGTGGG 
GCACCCCCTG AATCTAATCC CAGTGATGTG AAGGGCGAAG GGACAAGAAA GAACAATATG 
GAGATCACGT GGACGCCTAT GAATGCTACC TCTGCCTTTG GCCCCAACCT ACGCTACATT 
GTCAAGTGGC GACGGAGAGA GACCCGAGAG ACTTGGAACA ATGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC GCCTGTCTAC GTTCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC CGAGCCTGAC ACCATCATTG GGTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GAGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GTGACCGTCC CCGGGGCGTG GTGGCCCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTGGTGGT CAATGGGAGA GGTGACGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGGT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA GTGGGACCAC CCAGAGCACC CCAACGGAAT CCTGATTGGA 
TACATCCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCTGTG CAGAGAGCAG ACCCAGTGTC GCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC ACAGGTGGGC TCTGGAGAAG CAGCCACAGA GGAGTCCCCA 
GCACCTCCAA ATGAAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TTCTCATCGT CTGCTTCATC 
AAGAGGAGTC GAGGTGGCAA GTACCCAGTG CGGGAAAAGA AGGATGTCCC CTTGGGTCCT 
GAAGACCCCA AAGAAGAAGA TGGCTCATTT GACTACAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGATGGCACC ATCAAGCAGC AGGAGAGCGA TGACAGCCTG 
GTGGACTATG GCGAAGGCGG CGAGGGCCAG TTCAATGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA AAAAGGACAA GGAGGAAACG GAGGGCAATG AGAGCTCAGA GGCCACATCA 
CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110422.1
CDS	425..3934
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_030254562.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254562.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGGGCGAC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG 
AGGCTGACCT GGATCCCAGG GGATGACAAC AACAGCCCTA TCACAGACTA CGTCGTTCAG 
TTTGAAGAGG ACCAGTTCCA ACCAGGGGTG TGGCATGACC ACTCCAGGTT CCCAGGCAGC 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCA TATGTCAACT ACCAATTCAG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACCAGTGGG 
GCACCCCCTG AATCTAATCC CAGTGATGTG AAGGGCGAAG GGACAAGAAA GAACAATATG 
GAGATCACGT GGACGCCTAT GAATGCTACC TCTGCCTTTG GCCCCAACCT ACGCTACATT 
GTCAAGTGGC GACGGAGAGA GACCCGAGAG ACTTGGAACA ATGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC GCCTGTCTAC GTTCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC CGAGCCTGAC ACCATCATTG GGTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GAGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GTGACCGTCC CCGGGGCGTG GTGGCCCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTGGTGGT CAATGGGAGA GGTGACGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGGT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA GTGGGACCAC CCAGAGCACC CCAACGGAAT CCTGATTGGA 
TACATCCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCTGTG CAGAGAGCAG ACCCAGTGTC GCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC ACAGGTGGGC TCTGGAGAAG CAGCCACAGA GGAGTCCCCA 
GCACCTCCAA ATGAAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TTCTCATCGT CTGCTTCATC 
AAGAGGAGTC GAGGTGGCAA GTACCCAGTG CGGGAAAAGA AGGATGTCCC CTTGGGTCCT 
GAAGACCCCA AAGAAGAAGA TGGCTCATTT GACTACAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGATGGCACC ATCAAGCAGC AGGAGAGCGA TGACAGCCTG 
GTGGACTATG GCGAAGGCGG CGAGGGCCAG TTCAATGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA AAAAGGACAA GGAGGAAACG GAGGGCAATG AGAGCTCAGA GGCCACATCA 
CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( NM_001160316.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153788.1 Mus musculus neurofascin (Nfasc), transcript variant 2, mRNA.

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CloneID	OMu22790
Clone ID Related Accession (Same CDS sequence)	NM_001160316.1
Accession Version	NM_001160316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Mus musculus(house mouse)
Product	neurofascin isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC116691.12, AC159974.7 and AI847023.1. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The promoter and 5' UTR for this variant have not been determined. The resulting isoform (2) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences near the N-terminus, compared to isoform 1. Isoform 2 is also known as NF155, as described by Davis et al. (PMID: 8947556). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TACCCAAAGG GCGACCAGAC 
CGACCCAGGG ACCTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC 
CCAGGGGATG ACAACAACAG CCCTATCACA GACTACGTCG TTCAGTTTGA AGAGGACCAG 
TTCCAACCAG GGGTGTGGCA TGACCACTCC AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC 
CTCCATCTGT CCCCATATGT CAACTACCAA TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTTCC ATCCGAGCGG TACCGAACCA GTGGGGCACC CCCTGAATCT 
AATCCCAGTG ATGTGAAGGG CGAAGGGACA AGAAAGAACA ATATGGAGAT CACGTGGACG 
CCTATGAATG CTACCTCTGC CTTTGGCCCC AACCTACGCT ACATTGTCAA GTGGCGACGG 
AGAGAGACCC GAGAGACTTG GAACAATGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACGCCTG TCTACGTTCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTTGGGAAA 
GGCCCCGAGC CTGACACCAT CATTGGGTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGAGTC 
TACTCTGACA CGGTCCAGGG CCAGCTCAGA GAGTATCGAG CTTACTACTG GAGGGAAAGC 
AGTTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AGGCCAGCTT CCCTGGTGAC 
CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC CCCTACAGTA ACTACAAGCT GGAGATGGTG 
GTGGTCAATG GGAGAGGTGA CGGGCCTCGA AGTGAAACCA AGGAATTCAC CACCCCAGAA 
GGAGTACCCA GTGCCCCCAG GCGGTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC 
CTGGAGTGGG ACCACCCAGA GCACCCCAAC GGAATCCTGA TTGGATACAT CCTCAGATAC 
GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCT CTGTGCAGAG AGCAGACCCA GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC 
AGGACACAGG TGGGCTCTGG AGAAGCAGCC ACAGAGGAGT CCCCAGCACC TCCAAATGAA 
GCTACTCCAA CTGCAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TTCTCATCGT CTGCTTCATC 
AAGAGGAGTC GAGGTGGCAA GTACCCAGTG CGGGAAAAGA AGGATGTCCC CTTGGGTCCT 
GAAGACCCCA AAGAAGAAGA TGGCTCATTT GACTACAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGATGGCACC ATCAAGCAGC AGGAGAGCGA TGACAGCCTG 
GTGGACTATG GCGAAGGCGG CGAGGGCCAG TTCAATGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA AAAAGGACAA GGAGGAAACG GAGGGCAATG AGAGCTCAGA GGCCACATCA 
CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153788.1
CDS	91..3660
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001160316.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160316.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TACCCAAAGG GCGACCAGAC 
CGACCCAGGG ACCTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC 
CCAGGGGATG ACAACAACAG CCCTATCACA GACTACGTCG TTCAGTTTGA AGAGGACCAG 
TTCCAACCAG GGGTGTGGCA TGACCACTCC AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC 
CTCCATCTGT CCCCATATGT CAACTACCAA TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTTCC ATCCGAGCGG TACCGAACCA GTGGGGCACC CCCTGAATCT 
AATCCCAGTG ATGTGAAGGG CGAAGGGACA AGAAAGAACA ATATGGAGAT CACGTGGACG 
CCTATGAATG CTACCTCTGC CTTTGGCCCC AACCTACGCT ACATTGTCAA GTGGCGACGG 
AGAGAGACCC GAGAGACTTG GAACAATGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACGCCTG TCTACGTTCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTTGGGAAA 
GGCCCCGAGC CTGACACCAT CATTGGGTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGAGTC 
TACTCTGACA CGGTCCAGGG CCAGCTCAGA GAGTATCGAG CTTACTACTG GAGGGAAAGC 
AGTTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AGGCCAGCTT CCCTGGTGAC 
CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC CCCTACAGTA ACTACAAGCT GGAGATGGTG 
GTGGTCAATG GGAGAGGTGA CGGGCCTCGA AGTGAAACCA AGGAATTCAC CACCCCAGAA 
GGAGTACCCA GTGCCCCCAG GCGGTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC 
CTGGAGTGGG ACCACCCAGA GCACCCCAAC GGAATCCTGA TTGGATACAT CCTCAGATAC 
GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCT CTGTGCAGAG AGCAGACCCA GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC 
AGGACACAGG TGGGCTCTGG AGAAGCAGCC ACAGAGGAGT CCCCAGCACC TCCAAATGAA 
GCTACTCCAA CTGCAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TTCTCATCGT CTGCTTCATC 
AAGAGGAGTC GAGGTGGCAA GTACCCAGTG CGGGAAAAGA AGGATGTCCC CTTGGGTCCT 
GAAGACCCCA AAGAAGAAGA TGGCTCATTT GACTACAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGATGGCACC ATCAAGCAGC AGGAGAGCGA TGACAGCCTG 
GTGGACTATG GCGAAGGCGG CGAGGGCCAG TTCAATGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA AAAAGGACAA GGAGGAAACG GAGGGCAATG AGAGCTCAGA GGCCACATCA 
CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( NM_001160318.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153790.1 Mus musculus neurofascin (Nfasc), transcript variant 4, mRNA.

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CloneID	OMu22909
Clone ID Related Accession (Same CDS sequence)	NM_001160318.1
Accession Version	NM_001160318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Mus musculus(house mouse)
Product	neurofascin isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK129207.1, BC062092.1, AC159974.7 and AI847023.1. Transcript Variant: This variant (4) represents use of an alternate promoter and 5' UTR, and has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (4) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and lacks two segments in the N-terminal and central regions, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK129207.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCACC CGAGGAGTTG CAGAAAGAAC GCCCAGCTTC 
ATGTATCCCC AGGGCACATC GAGCAGTCAG ATGGTTCTCC GTGGCATGGA CCTGCTGCTG 
GAATGCATTG CCTCTGGCGT CCCAACACCA GACATTGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCATCTA ACAAGGCCAA GTTCGAGAAC TTTAATAAGG CTCTGCGCAT CACCAATGTC 
TCTGAAGAGG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCT CCATACTGGC TGGATGAGCC CAAGAACCTG 
ATCCTGGCTC CTGGGGAAGA TGGGAGGCTG GTATGCCGAG CCAATGGGAA CCCGAAGCCG 
ACCGTGCAGT GGATGGTGAA TGGAGAGCCT TTACAATCGG CACCACCCAA TCCCAACCGT 
GAGGTAGCTG GAGACACTAT CATCTTCCGG GATACTCAGA TCAGCAGCAG GGCAGTGTAC 
CAATGTAATA CATCCAATGA ACATGGCTAC CTGCTGGCCA ATGCCTTCGT CAGCGTGTTA 
GATGTACCCC CTCGGATGCT GTCTGCCCGC AACCAGCTCA TCAGGGTGAT CCTTTATAAC 
CGGACACGGC TGGACTGTCC GTTCTTTGGG TCTCCCATCC CAACACTCCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGCGGT AACTACCACG TCTACGAAAA CGGCAGTCTA 
GAAATCAAGA TGATTCGCAA AGAGGACCAA GGCATCTACA CCTGTGTGGC CACCAACATC 
CTGGGCAAAG CCGAAAATCA AGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACAGG 
ATGCCCGAGG ACCAGGTGGC CAAGAGGGGC ACCACGGTGC AGCTGGAGTG CCGCGTGAAA 
CATGACCCCT CCTTGAAGCT CACAGTCTCC TGGCTGAAGG ACGATGAGCC ACTCTACATT 
GGAAACAGGA TGAAGAAGGA AGATGACTCC CTGACGATCT TCGGAGTGGC AGAGCGGGAC 
CAGGGCAGTT ACACGTGTAT GGCCAGCACC GAGCTGGACC AGGACCTGGC AAAGGCCTAC 
CTCACTGTTC TAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153790.1
CDS	428..3901
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001160318.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160318.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCACC CGAGGAGTTG CAGAAAGAAC GCCCAGCTTC 
ATGTATCCCC AGGGCACATC GAGCAGTCAG ATGGTTCTCC GTGGCATGGA CCTGCTGCTG 
GAATGCATTG CCTCTGGCGT CCCAACACCA GACATTGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCATCTA ACAAGGCCAA GTTCGAGAAC TTTAATAAGG CTCTGCGCAT CACCAATGTC 
TCTGAAGAGG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCT CCATACTGGC TGGATGAGCC CAAGAACCTG 
ATCCTGGCTC CTGGGGAAGA TGGGAGGCTG GTATGCCGAG CCAATGGGAA CCCGAAGCCG 
ACCGTGCAGT GGATGGTGAA TGGAGAGCCT TTACAATCGG CACCACCCAA TCCCAACCGT 
GAGGTAGCTG GAGACACTAT CATCTTCCGG GATACTCAGA TCAGCAGCAG GGCAGTGTAC 
CAATGTAATA CATCCAATGA ACATGGCTAC CTGCTGGCCA ATGCCTTCGT CAGCGTGTTA 
GATGTACCCC CTCGGATGCT GTCTGCCCGC AACCAGCTCA TCAGGGTGAT CCTTTATAAC 
CGGACACGGC TGGACTGTCC GTTCTTTGGG TCTCCCATCC CAACACTCCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGCGGT AACTACCACG TCTACGAAAA CGGCAGTCTA 
GAAATCAAGA TGATTCGCAA AGAGGACCAA GGCATCTACA CCTGTGTGGC CACCAACATC 
CTGGGCAAAG CCGAAAATCA AGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACAGG 
ATGCCCGAGG ACCAGGTGGC CAAGAGGGGC ACCACGGTGC AGCTGGAGTG CCGCGTGAAA 
CATGACCCCT CCTTGAAGCT CACAGTCTCC TGGCTGAAGG ACGATGAGCC ACTCTACATT 
GGAAACAGGA TGAAGAAGGA AGATGACTCC CTGACGATCT TCGGAGTGGC AGAGCGGGAC 
CAGGGCAGTT ACACGTGTAT GGCCAGCACC GAGCTGGACC AGGACCTGGC AAAGGCCTAC 
CTCACTGTTC TAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( NM_182716.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_874385.1 Mus musculus neurofascin (Nfasc), transcript variant 1, mRNA.

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CloneID	OMu22927
Clone ID Related Accession (Same CDS sequence)	NM_182716.4
Accession Version	NM_182716.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	neurofascin isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124095.7, AC116691.12, AC159974.7 and AI847023.1. On May 21, 2009 this sequence version replaced NM_182716.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This isoform lacks the third fibronectin type 3 (FNIII) repeat and includes the mucin-like domain. Isoform 1 is also known as NF186, as described by Davis et al. (PMID: 8947556). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ543322.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACTATCA CCAAGCAGTC CGTGAAGGAC CACATCGTGG ACCCTCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG CAACCCCGCC CCCAGTTTTC ACTGGACTCG CAACAGCAGA 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGATCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAATACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TTGGCACTGC ACTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTGACACTA 
CAGTGCAACC CCCCACCCGG CCTCCCGTCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ACGCGCGCTT TCACTTCACC 
CACACCATTC AGCAGAAGAA CCCCTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACGC CCAGCTTCAT GTATCCCCAG GGCACATCGA GCAGTCAGAT GGTTCTCCGT 
GGCATGGACC TGCTGCTGGA ATGCATTGCC TCTGGCGTCC CAACACCAGA CATTGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTAAC AAGGCCAAGT TCGAGAACTT TAATAAGGCT 
CTGCGCATCA CCAATGTCTC TGAAGAGGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC ATACTGGCTG 
GATGAGCCCA AGAACCTGAT CCTGGCTCCT GGGGAAGATG GGAGGCTGGT ATGCCGAGCC 
AATGGGAACC CGAAGCCGAC CGTGCAGTGG ATGGTGAATG GAGAGCCTTT ACAATCGGCA 
CCACCCAATC CCAACCGTGA GGTAGCTGGA GACACTATCA TCTTCCGGGA TACTCAGATC 
AGCAGCAGGG CAGTGTACCA ATGTAATACA TCCAATGAAC ATGGCTACCT GCTGGCCAAT 
GCCTTCGTCA GCGTGTTAGA TGTACCCCCT CGGATGCTGT CTGCCCGCAA CCAGCTCATC 
AGGGTGATCC TTTATAACCG GACACGGCTG GACTGTCCGT TCTTTGGGTC TCCCATCCCA 
ACACTCCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGCGGTAA CTACCACGTC 
TACGAAAACG GCAGTCTAGA AATCAAGATG ATTCGCAAAG AGGACCAAGG CATCTACACC 
TGTGTGGCCA CCAACATCCT GGGCAAAGCC GAAAATCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACAGGAT GCCCGAGGAC CAGGTGGCCA AGAGGGGCAC CACGGTGCAG 
CTGGAGTGCC GCGTGAAACA TGACCCCTCC TTGAAGCTCA CAGTCTCCTG GCTGAAGGAC 
GATGAGCCAC TCTACATTGG AAACAGGATG AAGAAGGAAG ATGACTCCCT GACGATCTTC 
GGAGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTATGG CCAGCACCGA GCTGGACCAG 
GACCTGGCAA AGGCCTACCT CACTGTTCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTAC CCAAAGGGCG ACCAGACCGA CCCAGGGACC TGGAGCTCAC TGACCTGGCT 
GAGAGGAGTG TGAGGCTGAC CTGGATCCCA GGGGATGACA ACAACAGCCC TATCACAGAC 
TACGTCGTTC AGTTTGAAGA GGACCAGTTC CAACCAGGGG TGTGGCATGA CCACTCCAGG 
TTCCCAGGCA GCGTCAACTC AGCCGTCCTC CATCTGTCCC CATATGTCAA CTACCAATTC 
AGAGTCATCG CTGTCAACGA GGTTGGGAGC AGCCACCCCA GCCTTCCATC CGAGCGGTAC 
CGAACCAGTG GGGCACCCCC TGAATCTAAT CCCAGTGATG TGAAGGGCGA AGGGACAAGA 
AAGAACAATA TGGAGATCAC GTGGACGCCT ATGAATGCTA CCTCTGCCTT TGGCCCCAAC 
CTACGCTACA TTGTCAAGTG GCGACGGAGA GAGACCCGAG AGACTTGGAA CAATGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACGCCTGTCT ACGTTCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT TGGGAAAGGC CCCGAGCCTG ACACCATCAT TGGGTACTCC 
GGAGAAGATT TACCCAGTGC CCCCAGGCGG TTCAGAGTCC GACAGCCCAA CCTGGAGACC 
ATCAACCTGG AGTGGGACCA CCCAGAGCAC CCCAACGGAA TCCTGATTGG ATACATCCTC 
AGATACGTGC CCTTTAATGG AACCAAACTG GGAAAGCAGA TGGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCTCTGT GCAGAGAGCA GACCCAGTGT CGCGTTACCG CTTCTCCCTC 
AGTGCCAGGA CACAGGTGGG CTCTGGAGAA GCAGCCACAG AGGAGTCCCC AGCACCTCCA 
AATGAAGCTA CTCCAACTGC AGCTCCTCCC ACGTTGCCCC CGACTACTGT GGGTACCACA 
GGCCTTGTGA GCAGTACTGA TGCTACTGCC CTTGCTGCCA CCAGTGAAGC CACAACAGTT 
CCCATCATTC CAACCGTCGT ACCTACCACC GTCGCCACCA CCATTGCCAC AACTACTACA 
ACCACTGCCG CCACCACCAC CACCACCACT ACGGAGAGCC CTCCCACTAC CACTGCTGGG 
ACTAAGATTC ACGAAACCGC CCCCGACGAG CAGTCCATTT GGAACGTCAC AGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCA GGCCTGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCTATACAGC TGACAGACCT CTTTCCCGGG ATGACGTACA CGTTGCGGGT GTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT 
AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT 
CTGGTGCTGA TCCTTCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAGTACCCA 
GTGCGGGAAA AGAAGGATGT CCCCTTGGGT CCTGAAGACC CCAAAGAAGA AGATGGCTCA 
TTTGACTACA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGATGGC 
ACCATCAAGC AGCAGGAGAG CGATGACAGC CTGGTGGACT ATGGCGAAGG CGGCGAGGGC 
CAGTTCAATG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAAAAAGGA CAAGGAGGAA 
ACGGAGGGCA ATGAGAGCTC AGAGGCCACA TCACCAGTCA ATGCCATCTA TTCCCTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_874385.1
CDS	327..4049
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_182716.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182716.4

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACTATCA CCAAGCAGTC CGTGAAGGAC CACATCGTGG ACCCTCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG CAACCCCGCC CCCAGTTTTC ACTGGACTCG CAACAGCAGA 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGATCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAATACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TTGGCACTGC ACTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTGACACTA 
CAGTGCAACC CCCCACCCGG CCTCCCGTCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ACGCGCGCTT TCACTTCACC 
CACACCATTC AGCAGAAGAA CCCCTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA 
GAAAGAACGC CCAGCTTCAT GTATCCCCAG GGCACATCGA GCAGTCAGAT GGTTCTCCGT 
GGCATGGACC TGCTGCTGGA ATGCATTGCC TCTGGCGTCC CAACACCAGA CATTGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTAAC AAGGCCAAGT TCGAGAACTT TAATAAGGCT 
CTGCGCATCA CCAATGTCTC TGAAGAGGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC ATACTGGCTG 
GATGAGCCCA AGAACCTGAT CCTGGCTCCT GGGGAAGATG GGAGGCTGGT ATGCCGAGCC 
AATGGGAACC CGAAGCCGAC CGTGCAGTGG ATGGTGAATG GAGAGCCTTT ACAATCGGCA 
CCACCCAATC CCAACCGTGA GGTAGCTGGA GACACTATCA TCTTCCGGGA TACTCAGATC 
AGCAGCAGGG CAGTGTACCA ATGTAATACA TCCAATGAAC ATGGCTACCT GCTGGCCAAT 
GCCTTCGTCA GCGTGTTAGA TGTACCCCCT CGGATGCTGT CTGCCCGCAA CCAGCTCATC 
AGGGTGATCC TTTATAACCG GACACGGCTG GACTGTCCGT TCTTTGGGTC TCCCATCCCA 
ACACTCCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGCGGTAA CTACCACGTC 
TACGAAAACG GCAGTCTAGA AATCAAGATG ATTCGCAAAG AGGACCAAGG CATCTACACC 
TGTGTGGCCA CCAACATCCT GGGCAAAGCC GAAAATCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACAGGAT GCCCGAGGAC CAGGTGGCCA AGAGGGGCAC CACGGTGCAG 
CTGGAGTGCC GCGTGAAACA TGACCCCTCC TTGAAGCTCA CAGTCTCCTG GCTGAAGGAC 
GATGAGCCAC TCTACATTGG AAACAGGATG AAGAAGGAAG ATGACTCCCT GACGATCTTC 
GGAGTGGCAG AGCGGGACCA GGGCAGTTAC ACGTGTATGG CCAGCACCGA GCTGGACCAG 
GACCTGGCAA AGGCCTACCT CACTGTTCTA GCTGATCAGG CCACTCCAAC TAACCGTTTG 
GCTGCCCTAC CCAAAGGGCG ACCAGACCGA CCCAGGGACC TGGAGCTCAC TGACCTGGCT 
GAGAGGAGTG TGAGGCTGAC CTGGATCCCA GGGGATGACA ACAACAGCCC TATCACAGAC 
TACGTCGTTC AGTTTGAAGA GGACCAGTTC CAACCAGGGG TGTGGCATGA CCACTCCAGG 
TTCCCAGGCA GCGTCAACTC AGCCGTCCTC CATCTGTCCC CATATGTCAA CTACCAATTC 
AGAGTCATCG CTGTCAACGA GGTTGGGAGC AGCCACCCCA GCCTTCCATC CGAGCGGTAC 
CGAACCAGTG GGGCACCCCC TGAATCTAAT CCCAGTGATG TGAAGGGCGA AGGGACAAGA 
AAGAACAATA TGGAGATCAC GTGGACGCCT ATGAATGCTA CCTCTGCCTT TGGCCCCAAC 
CTACGCTACA TTGTCAAGTG GCGACGGAGA GAGACCCGAG AGACTTGGAA CAATGTCACA 
GTGTGGGGCT CTCGCTACGT GGTGGGGCAG ACGCCTGTCT ACGTTCCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT TGGGAAAGGC CCCGAGCCTG ACACCATCAT TGGGTACTCC 
GGAGAAGATT TACCCAGTGC CCCCAGGCGG TTCAGAGTCC GACAGCCCAA CCTGGAGACC 
ATCAACCTGG AGTGGGACCA CCCAGAGCAC CCCAACGGAA TCCTGATTGG ATACATCCTC 
AGATACGTGC CCTTTAATGG AACCAAACTG GGAAAGCAGA TGGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCTCTGT GCAGAGAGCA GACCCAGTGT CGCGTTACCG CTTCTCCCTC 
AGTGCCAGGA CACAGGTGGG CTCTGGAGAA GCAGCCACAG AGGAGTCCCC AGCACCTCCA 
AATGAAGCTA CTCCAACTGC AGCTCCTCCC ACGTTGCCCC CGACTACTGT GGGTACCACA 
GGCCTTGTGA GCAGTACTGA TGCTACTGCC CTTGCTGCCA CCAGTGAAGC CACAACAGTT 
CCCATCATTC CAACCGTCGT ACCTACCACC GTCGCCACCA CCATTGCCAC AACTACTACA 
ACCACTGCCG CCACCACCAC CACCACCACT ACGGAGAGCC CTCCCACTAC CACTGCTGGG 
ACTAAGATTC ACGAAACCGC CCCCGACGAG CAGTCCATTT GGAACGTCAC AGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCA GGCCTGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCTATACAGC TGACAGACCT CTTTCCCGGG ATGACGTACA CGTTGCGGGT GTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT 
AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT 
CTGGTGCTGA TCCTTCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAGTACCCA 
GTGCGGGAAA AGAAGGATGT CCCCTTGGGT CCTGAAGACC CCAAAGAAGA AGATGGCTCA 
TTTGACTACA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGATGGC 
ACCATCAAGC AGCAGGAGAG CGATGACAGC CTGGTGGACT ATGGCGAAGG CGGCGAGGGC 
CAGTTCAATG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAAAAAGGA CAAGGAGGAA 
ACGGAGGGCA ATGAGAGCTC AGAGGCCACA TCACCAGTCA ATGCCATCTA TTCCCTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( NM_001160317.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153789.1 Mus musculus neurofascin (Nfasc), transcript variant 3, mRNA.

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CloneID	OMu22945
Clone ID Related Accession (Same CDS sequence)	NM_001160317.1
Accession Version	NM_001160317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Mus musculus(house mouse)
Product	neurofascin isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB659942.1, AK138636.1, AK129207.1, BC062092.1, AC159974.7 and AI847023.1. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences in the N-terminal and central regions, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGGGCGAC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG 
AGGCTGACCT GGATCCCAGG GGATGACAAC AACAGCCCTA TCACAGACTA CGTCGTTCAG 
TTTGAAGAGG ACCAGTTCCA ACCAGGGGTG TGGCATGACC ACTCCAGGTT CCCAGGCAGC 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCA TATGTCAACT ACCAATTCAG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACCAGTGGG 
GCACCCCCTG AATCTAATCC CAGTGATGTG AAGGGCGAAG GGACAAGAAA GAACAATATG 
GAGATCACGT GGACGCCTAT GAATGCTACC TCTGCCTTTG GCCCCAACCT ACGCTACATT 
GTCAAGTGGC GACGGAGAGA GACCCGAGAG ACTTGGAACA ATGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC GCCTGTCTAC GTTCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC CGAGCCTGAC ACCATCATTG GGTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GAGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GTGACCGTCC CCGGGGCGTG GTGGCCCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTGGTGGT CAATGGGAGA GGTGACGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGGT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA GTGGGACCAC CCAGAGCACC CCAACGGAAT CCTGATTGGA 
TACATCCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCTGTG CAGAGAGCAG ACCCAGTGTC GCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC ACAGGTGGGC TCTGGAGAAG CAGCCACAGA GGAGTCCCCA 
GCACCTCCAA ATGAAGCTAC TCCAACTGCA GCTTACACCA ATAACCAGGC AGACATCGCC 
ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTTCTC 
ATCGTCTGCT TCATCAAGAG GAGTCGAGGT GGCAAGTACC CAGTGCGGGA AAAGAAGGAT 
GTCCCCTTGG GTCCTGAAGA CCCCAAAGAA GAAGATGGCT CATTTGACTA CAGTGATGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGATG GCACCATCAA GCAGCAGGAG 
AGCGATGACA GCCTGGTGGA CTATGGCGAA GGCGGCGAGG GCCAGTTCAA TGAAGATGGC 
TCCTTTATTG GCCAGTACAC TGTCAAAAAG GACAAGGAGG AAACGGAGGG CAATGAGAGC 
TCAGAGGCCA CATCACCAGT CAATGCCATC TATTCCCTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153789.1
CDS	327..3851
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001160317.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160317.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGGGCGAC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG 
AGGCTGACCT GGATCCCAGG GGATGACAAC AACAGCCCTA TCACAGACTA CGTCGTTCAG 
TTTGAAGAGG ACCAGTTCCA ACCAGGGGTG TGGCATGACC ACTCCAGGTT CCCAGGCAGC 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCA TATGTCAACT ACCAATTCAG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACCAGTGGG 
GCACCCCCTG AATCTAATCC CAGTGATGTG AAGGGCGAAG GGACAAGAAA GAACAATATG 
GAGATCACGT GGACGCCTAT GAATGCTACC TCTGCCTTTG GCCCCAACCT ACGCTACATT 
GTCAAGTGGC GACGGAGAGA GACCCGAGAG ACTTGGAACA ATGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC GCCTGTCTAC GTTCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC CGAGCCTGAC ACCATCATTG GGTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GAGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GTGACCGTCC CCGGGGCGTG GTGGCCCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTGGTGGT CAATGGGAGA GGTGACGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGGT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA GTGGGACCAC CCAGAGCACC CCAACGGAAT CCTGATTGGA 
TACATCCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCTGTG CAGAGAGCAG ACCCAGTGTC GCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC ACAGGTGGGC TCTGGAGAAG CAGCCACAGA GGAGTCCCCA 
GCACCTCCAA ATGAAGCTAC TCCAACTGCA GCTTACACCA ATAACCAGGC AGACATCGCC 
ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTTCTC 
ATCGTCTGCT TCATCAAGAG GAGTCGAGGT GGCAAGTACC CAGTGCGGGA AAAGAAGGAT 
GTCCCCTTGG GTCCTGAAGA CCCCAAAGAA GAAGATGGCT CATTTGACTA CAGTGATGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGATG GCACCATCAA GCAGCAGGAG 
AGCGATGACA GCCTGGTGGA CTATGGCGAA GGCGGCGAGG GCCAGTTCAA TGAAGATGGC 
TCCTTTATTG GCCAGTACAC TGTCAAAAAG GACAAGGAGG AAACGGAGGG CAATGAGAGC 
TCAGAGGCCA CATCACCAGT CAATGCCATC TATTCCCTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006529644.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529707.2 Mus musculus neurofascin (Nfasc), transcript variant X11, mRNA.

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CloneID	OMu74375
Clone ID Related Accession (Same CDS sequence)	XM_006529644.2 , XM_006529644.3
Accession Version	XM_006529644.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006529644.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529707.2
CDS	55..3948
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006529644.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529644.2

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTTACACCAA TAACCAGGCA 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTTCTCA TCGTCTGCTT CATCAAGAGG AGTCGAGGTG GCAAGTACCC AGTGCGGGAA 
AAGAAGGATG TCCCCTTGGG TCCTGAAGAC CCCAAAGAAG AAGATGGCTC ATTTGACTAC 
AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGATGG CACCATCAAG 
CAGCAGGAGA GCGATGACAG CCTGGTGGAC TATGGCGAAG GCGGCGAGGG CCAGTTCAAT 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAAAAAGG ACAAGGAGGA AACGGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCACCAGTC AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248017.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246319.1 Mus musculus neurofascin (Nfasc), transcript variant X12, mRNA.

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CloneID	OMu74376
Clone ID Related Accession (Same CDS sequence)	XM_011248017.2 , XM_011248017.1
Accession Version	XM_011248017.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTTAC ACCAATAACC AGGCAGACAT CGCCACCCAG 
GGCTGGTTCA TCGGGCTCAT GTGTGCCATT GCCCTTCTGG TGCTGATCCT TCTCATCGTC 
TGCTTCATCA AGAGGAGTCG AGGTGGCAAG TACCCAGTGC GGGAAAAGAA GGATGTCCCC 
TTGGGTCCTG AAGACCCCAA AGAAGAAGAT GGCTCATTTG ACTACAGTGA TGAGGACAAC 
AAGCCCCTGC AGGGCAGCCA GACATCTCTG GATGGCACCA TCAAGCAGCA GGAGAGCGAT 
GACAGCCTGG TGGACTATGG CGAAGGCGGC GAGGGCCAGT TCAATGAAGA TGGCTCCTTT 
ATTGGCCAGT ACACTGTCAA AAAGGACAAG GAGGAAACGG AGGGCAATGA GAGCTCAGAG 
GCCACATCAC CAGTCAATGC CATCTATTCC CTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246319.1
CDS	55..3933
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248017.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248017.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTTAC ACCAATAACC AGGCAGACAT CGCCACCCAG 
GGCTGGTTCA TCGGGCTCAT GTGTGCCATT GCCCTTCTGG TGCTGATCCT TCTCATCGTC 
TGCTTCATCA AGAGGAGTCG AGGTGGCAAG TACCCAGTGC GGGAAAAGAA GGATGTCCCC 
TTGGGTCCTG AAGACCCCAA AGAAGAAGAT GGCTCATTTG ACTACAGTGA TGAGGACAAC 
AAGCCCCTGC AGGGCAGCCA GACATCTCTG GATGGCACCA TCAAGCAGCA GGAGAGCGAT 
GACAGCCTGG TGGACTATGG CGAAGGCGGC GAGGGCCAGT TCAATGAAGA TGGCTCCTTT 
ATTGGCCAGT ACACTGTCAA AAAGGACAAG GAGGAAACGG AGGGCAATGA GAGCTCAGAG 
GCCACATCAC CAGTCAATGC CATCTATTCC CTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006529646.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529709.2 Mus musculus neurofascin (Nfasc), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu74377
Clone ID Related Accession (Same CDS sequence)	XM_006529646.3 , XM_006529646.2
Accession Version	XM_006529646.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006529646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG CTGATCAGGC CACTCCAACT AACCGTTTGG CTGCCCTACC CAAAGGGCGA 
CCAGACCGAC CCAGGGACCT GGAGCTCACT GACCTGGCTG AGAGGAGTGT GAGGCTGACC 
TGGATCCCAG GGGATGACAA CAACAGCCCT ATCACAGACT ACGTCGTTCA GTTTGAAGAG 
GACCAGTTCC AACCAGGGGT GTGGCATGAC CACTCCAGGT TCCCAGGCAG CGTCAACTCA 
GCCGTCCTCC ATCTGTCCCC ATATGTCAAC TACCAATTCA GAGTCATCGC TGTCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTTCCATCC GAGCGGTACC GAACCAGTGG GGCACCCCCT 
GAATCTAATC CCAGTGATGT GAAGGGCGAA GGGACAAGAA AGAACAATAT GGAGATCACG 
TGGACGCCTA TGAATGCTAC CTCTGCCTTT GGCCCCAACC TACGCTACAT TGTCAAGTGG 
CGACGGAGAG AGACCCGAGA GACTTGGAAC AATGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CGCCTGTCTA CGTTCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTT 
GGGAAAGGCC CCGAGCCTGA CACCATCATT GGGTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAAT CCGAGTCCTG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGAGTCTACT CTGACACGGT CCAGGGCCAG CTCAGAGAGT ATCGAGCTTA CTACTGGAGG 
GAAAGCAGTT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAGGC CAGCTTCCCT 
GGTGACCGTC CCCGGGGCGT GGTGGCCCGC CTGTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTGGTGG TCAATGGGAG AGGTGACGGG CCTCGAAGTG AAACCAAGGA ATTCACCACC 
CCAGAAGGAG TACCCAGTGC CCCCAGGCGG TTCAGAGTCC GACAGCCCAA CCTGGAGACC 
ATCAACCTGG AGTGGGACCA CCCAGAGCAC CCCAACGGAA TCCTGATTGG ATACATCCTC 
AGATACGTGC CCTTTAATGG AACCAAACTG GGAAAGCAGA TGGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCTCTGT GCAGAGAGCA GACCCAGTGT CGCGTTACCG CTTCTCCCTC 
AGTGCCAGGA CACAGGTGGG CTCTGGAGAA GCAGCCACAG AGGAGTCCCC AGCACCTCCA 
AATGAAGCTA CTCCAACTGC AGCTTACACC AATAACCAGG CAGACATCGC CACCCAGGGC 
TGGTTCATCG GGCTCATGTG TGCCATTGCC CTTCTGGTGC TGATCCTTCT CATCGTCTGC 
TTCATCAAGA GGAGTCGAGG TGGCAAGTAC CCAGTGCGGG AAAAGAAGGA TGTCCCCTTG 
GGTCCTGAAG ACCCCAAAGA AGAAGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC ATCTCTGGAT GGCACCATCA AGCAGCAGGA GAGCGATGAC 
AGCCTGGTGG ACTATGGCGA AGGCGGCGAG GGCCAGTTCA ATGAAGATGG CTCCTTTATT 
GGCCAGTACA CTGTCAAAAA GGACAAGGAG GAAACGGAGG GCAATGAGAG CTCAGAGGCC 
ACATCACCAG TCAATGCCAT CTATTCCCTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529709.2
CDS	55..3930
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006529646.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529646.2

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG CTGATCAGGC CACTCCAACT AACCGTTTGG CTGCCCTACC CAAAGGGCGA 
CCAGACCGAC CCAGGGACCT GGAGCTCACT GACCTGGCTG AGAGGAGTGT GAGGCTGACC 
TGGATCCCAG GGGATGACAA CAACAGCCCT ATCACAGACT ACGTCGTTCA GTTTGAAGAG 
GACCAGTTCC AACCAGGGGT GTGGCATGAC CACTCCAGGT TCCCAGGCAG CGTCAACTCA 
GCCGTCCTCC ATCTGTCCCC ATATGTCAAC TACCAATTCA GAGTCATCGC TGTCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTTCCATCC GAGCGGTACC GAACCAGTGG GGCACCCCCT 
GAATCTAATC CCAGTGATGT GAAGGGCGAA GGGACAAGAA AGAACAATAT GGAGATCACG 
TGGACGCCTA TGAATGCTAC CTCTGCCTTT GGCCCCAACC TACGCTACAT TGTCAAGTGG 
CGACGGAGAG AGACCCGAGA GACTTGGAAC AATGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CGCCTGTCTA CGTTCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTT 
GGGAAAGGCC CCGAGCCTGA CACCATCATT GGGTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAAT CCGAGTCCTG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGAGTCTACT CTGACACGGT CCAGGGCCAG CTCAGAGAGT ATCGAGCTTA CTACTGGAGG 
GAAAGCAGTT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAGGC CAGCTTCCCT 
GGTGACCGTC CCCGGGGCGT GGTGGCCCGC CTGTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTGGTGG TCAATGGGAG AGGTGACGGG CCTCGAAGTG AAACCAAGGA ATTCACCACC 
CCAGAAGGAG TACCCAGTGC CCCCAGGCGG TTCAGAGTCC GACAGCCCAA CCTGGAGACC 
ATCAACCTGG AGTGGGACCA CCCAGAGCAC CCCAACGGAA TCCTGATTGG ATACATCCTC 
AGATACGTGC CCTTTAATGG AACCAAACTG GGAAAGCAGA TGGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCTCTGT GCAGAGAGCA GACCCAGTGT CGCGTTACCG CTTCTCCCTC 
AGTGCCAGGA CACAGGTGGG CTCTGGAGAA GCAGCCACAG AGGAGTCCCC AGCACCTCCA 
AATGAAGCTA CTCCAACTGC AGCTTACACC AATAACCAGG CAGACATCGC CACCCAGGGC 
TGGTTCATCG GGCTCATGTG TGCCATTGCC CTTCTGGTGC TGATCCTTCT CATCGTCTGC 
TTCATCAAGA GGAGTCGAGG TGGCAAGTAC CCAGTGCGGG AAAAGAAGGA TGTCCCCTTG 
GGTCCTGAAG ACCCCAAAGA AGAAGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC ATCTCTGGAT GGCACCATCA AGCAGCAGGA GAGCGATGAC 
AGCCTGGTGG ACTATGGCGA AGGCGGCGAG GGCCAGTTCA ATGAAGATGG CTCCTTTATT 
GGCCAGTACA CTGTCAAAAA GGACAAGGAG GAAACGGAGG GCAATGAGAG CTCAGAGGCC 
ACATCACCAG TCAATGCCAT CTATTCCCTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248018.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246320.1 Mus musculus neurofascin (Nfasc), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OMu74379
Clone ID Related Accession (Same CDS sequence)	XM_011248018.1 , XM_011248018.2
Accession Version	XM_011248018.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG GGCGACCAGA CCGACCCAGG GACCTGGAGC TCACTGACCT GGCTGAGAGG 
AGTGTGAGGC TGACCTGGAT CCCAGGGGAT GACAACAACA GCCCTATCAC AGACTACGTC 
GTTCAGTTTG AAGAGGACCA GTTCCAACCA GGGGTGTGGC ATGACCACTC CAGGTTCCCA 
GGCAGCGTCA ACTCAGCCGT CCTCCATCTG TCCCCATATG TCAACTACCA ATTCAGAGTC 
ATCGCTGTCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTTC CATCCGAGCG GTACCGAACC 
AGTGGGGCAC CCCCTGAATC TAATCCCAGT GATGTGAAGG GCGAAGGGAC AAGAAAGAAC 
AATATGGAGA TCACGTGGAC GCCTATGAAT GCTACCTCTG CCTTTGGCCC CAACCTACGC 
TACATTGTCA AGTGGCGACG GAGAGAGACC CGAGAGACTT GGAACAATGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACGCCT GTCTACGTTC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTTGGGAA AGGCCCCGAG CCTGACACCA TCATTGGGTA CTCCGGAGAA 
GATTATCCCA GGGCTGCGCC CACTGAAGTT AAAATCCGAG TCCTGAACAG CACAGCCATC 
AGCCTTCAGT GGAACCGAGT CTACTCTGAC ACGGTCCAGG GCCAGCTCAG AGAGTATCGA 
GCTTACTACT GGAGGGAAAG CAGTTTGCTG AAGAACCTGT GGGTGTCTCA GAAGAGACAG 
CAGGCCAGCT TCCCTGGTGA CCGTCCCCGG GGCGTGGTGG CCCGCCTGTT CCCCTACAGT 
AACTACAAGC TGGAGATGGT GGTGGTCAAT GGGAGAGGTG ACGGGCCTCG AAGTGAAACC 
AAGGAATTCA CCACCCCAGA AGGAGTACCC AGTGCCCCCA GGCGGTTCAG AGTCCGACAG 
CCCAACCTGG AGACCATCAA CCTGGAGTGG GACCACCCAG AGCACCCCAA CGGAATCCTG 
ATTGGATACA TCCTCAGATA CGTGCCCTTT AATGGAACCA AACTGGGAAA GCAGATGGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC TCTGTGCAGA GAGCAGACCC AGTGTCGCGT 
TACCGCTTCT CCCTCAGTGC CAGGACACAG GTGGGCTCTG GAGAAGCAGC CACAGAGGAG 
TCCCCAGCAC CTCCAAATGA AGCTACTCCA ACTGCAGCTT ACACCAATAA CCAGGCAGAC 
ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGTGCCA TTGCCCTTCT GGTGCTGATC 
CTTCTCATCG TCTGCTTCAT CAAGAGGAGT CGAGGTGGCA AGTACCCAGT GCGGGAAAAG 
AAGGATGTCC CCTTGGGTCC TGAAGACCCC AAAGAAGAAG ATGGCTCATT TGACTACAGT 
GATGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCTC TGGATGGCAC CATCAAGCAG 
CAGGAGAGCG ATGACAGCCT GGTGGACTAT GGCGAAGGCG GCGAGGGCCA GTTCAATGAA 
GATGGCTCCT TTATTGGCCA GTACACTGTC AAAAAGGACA AGGAGGAAAC GGAGGGCAAT 
GAGAGCTCAG AGGCCACATC ACCAGTCAAT GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246320.1
CDS	55..3885
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248018.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248018.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG GGCGACCAGA CCGACCCAGG GACCTGGAGC TCACTGACCT GGCTGAGAGG 
AGTGTGAGGC TGACCTGGAT CCCAGGGGAT GACAACAACA GCCCTATCAC AGACTACGTC 
GTTCAGTTTG AAGAGGACCA GTTCCAACCA GGGGTGTGGC ATGACCACTC CAGGTTCCCA 
GGCAGCGTCA ACTCAGCCGT CCTCCATCTG TCCCCATATG TCAACTACCA ATTCAGAGTC 
ATCGCTGTCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTTC CATCCGAGCG GTACCGAACC 
AGTGGGGCAC CCCCTGAATC TAATCCCAGT GATGTGAAGG GCGAAGGGAC AAGAAAGAAC 
AATATGGAGA TCACGTGGAC GCCTATGAAT GCTACCTCTG CCTTTGGCCC CAACCTACGC 
TACATTGTCA AGTGGCGACG GAGAGAGACC CGAGAGACTT GGAACAATGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACGCCT GTCTACGTTC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTTGGGAA AGGCCCCGAG CCTGACACCA TCATTGGGTA CTCCGGAGAA 
GATTATCCCA GGGCTGCGCC CACTGAAGTT AAAATCCGAG TCCTGAACAG CACAGCCATC 
AGCCTTCAGT GGAACCGAGT CTACTCTGAC ACGGTCCAGG GCCAGCTCAG AGAGTATCGA 
GCTTACTACT GGAGGGAAAG CAGTTTGCTG AAGAACCTGT GGGTGTCTCA GAAGAGACAG 
CAGGCCAGCT TCCCTGGTGA CCGTCCCCGG GGCGTGGTGG CCCGCCTGTT CCCCTACAGT 
AACTACAAGC TGGAGATGGT GGTGGTCAAT GGGAGAGGTG ACGGGCCTCG AAGTGAAACC 
AAGGAATTCA CCACCCCAGA AGGAGTACCC AGTGCCCCCA GGCGGTTCAG AGTCCGACAG 
CCCAACCTGG AGACCATCAA CCTGGAGTGG GACCACCCAG AGCACCCCAA CGGAATCCTG 
ATTGGATACA TCCTCAGATA CGTGCCCTTT AATGGAACCA AACTGGGAAA GCAGATGGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC TCTGTGCAGA GAGCAGACCC AGTGTCGCGT 
TACCGCTTCT CCCTCAGTGC CAGGACACAG GTGGGCTCTG GAGAAGCAGC CACAGAGGAG 
TCCCCAGCAC CTCCAAATGA AGCTACTCCA ACTGCAGCTT ACACCAATAA CCAGGCAGAC 
ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGTGCCA TTGCCCTTCT GGTGCTGATC 
CTTCTCATCG TCTGCTTCAT CAAGAGGAGT CGAGGTGGCA AGTACCCAGT GCGGGAAAAG 
AAGGATGTCC CCTTGGGTCC TGAAGACCCC AAAGAAGAAG ATGGCTCATT TGACTACAGT 
GATGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCTC TGGATGGCAC CATCAAGCAG 
CAGGAGAGCG ATGACAGCCT GGTGGACTAT GGCGAAGGCG GCGAGGGCCA GTTCAATGAA 
GATGGCTCCT TTATTGGCCA GTACACTGTC AAAAAGGACA AGGAGGAAAC GGAGGGCAAT 
GAGAGCTCAG AGGCCACATC ACCAGTCAAT GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006529651.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529714.2 Mus musculus neurofascin (Nfasc), transcript variant X18, mRNA.

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CloneID	OMu74380
Clone ID Related Accession (Same CDS sequence)	XM_006529651.2 , XM_006529651.3
Accession Version	XM_006529651.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006529651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG GGCGACCAGA CCGACCCAGG GACCTGGAGC TCACTGACCT GGCTGAGAGG 
AGTGTGAGGC TGACCTGGAT CCCAGGGGAT GACAACAACA GCCCTATCAC AGACTACGTC 
GTTCAGTTTG AAGAGGACCA GTTCCAACCA GGGGTGTGGC ATGACCACTC CAGGTTCCCA 
GGCAGCGTCA ACTCAGCCGT CCTCCATCTG TCCCCATATG TCAACTACCA ATTCAGAGTC 
ATCGCTGTCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTTC CATCCGAGCG GTACCGAACC 
AGTGGGGCAC CCCCTGAATC TAATCCCAGT GATGTGAAGG GCGAAGGGAC AAGAAAGAAC 
AATATGGAGA TCACGTGGAC GCCTATGAAT GCTACCTCTG CCTTTGGCCC CAACCTACGC 
TACATTGTCA AGTGGCGACG GAGAGAGACC CGAGAGACTT GGAACAATGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACGCCT GTCTACGTTC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTTGGGAA AGGCCCCGAG CCTGACACCA TCATTGGGTA CTCCGGAGAA 
GATTATCCCA GGGCTGCGCC CACTGAAGTT AAAATCCGAG TCCTGAACAG CACAGCCATC 
AGCCTTCAGT GGAACCGAGT CTACTCTGAC ACGGTCCAGG GCCAGCTCAG AGAGTATCGA 
GCTTACTACT GGAGGGAAAG CAGTTTGCTG AAGAACCTGT GGGTGTCTCA GAAGAGACAG 
CAGGCCAGCT TCCCTGGTGA CCGTCCCCGG GGCGTGGTGG CCCGCCTGTT CCCCTACAGT 
AACTACAAGC TGGAGATGGT GGTGGTCAAT GGGAGAGGTG ACGGGCCTCG AAGTGAAACC 
AAGGAATTCA CCACCCCAGA AGGAGTACCC AGTGCCCCCA GGCGGTTCAG AGTCCGACAG 
CCCAACCTGG AGACCATCAA CCTGGAGTGG GACCACCCAG AGCACCCCAA CGGAATCCTG 
ATTGGATACA TCCTCAGATA CGTGCCCTTT AATGGAACCA AACTGGGAAA GCAGATGGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC TCTGTGCAGA GAGCAGACCC AGTGTCGCGT 
TACCGCTTCT CCCTCAGTGC CAGGACACAG GTGGGCTCTG GAGAAGCAGC CACAGAGGAG 
TCCCCAGCAC CTCCAAATGA AGCTTACACC AATAACCAGG CAGACATCGC CACCCAGGGC 
TGGTTCATCG GGCTCATGTG TGCCATTGCC CTTCTGGTGC TGATCCTTCT CATCGTCTGC 
TTCATCAAGA GGAGTCGAGG TGGCAAGTAC CCAGTGCGGG AAAAGAAGGA TGTCCCCTTG 
GGTCCTGAAG ACCCCAAAGA AGAAGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC ATCTCTGGAT GGCACCATCA AGCAGCAGGA GAGCGATGAC 
AGCCTGGTGG ACTATGGCGA AGGCGGCGAG GGCCAGTTCA ATGAAGATGG CTCCTTTATT 
GGCCAGTACA CTGTCAAAAA GGACAAGGAG GAAACGGAGG GCAATGAGAG CTCAGAGGCC 
ACATCACCAG TCAATGCCAT CTATTCCCTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529714.2
CDS	55..3870
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006529651.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529651.2

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG GGCGACCAGA CCGACCCAGG GACCTGGAGC TCACTGACCT GGCTGAGAGG 
AGTGTGAGGC TGACCTGGAT CCCAGGGGAT GACAACAACA GCCCTATCAC AGACTACGTC 
GTTCAGTTTG AAGAGGACCA GTTCCAACCA GGGGTGTGGC ATGACCACTC CAGGTTCCCA 
GGCAGCGTCA ACTCAGCCGT CCTCCATCTG TCCCCATATG TCAACTACCA ATTCAGAGTC 
ATCGCTGTCA ACGAGGTTGG GAGCAGCCAC CCCAGCCTTC CATCCGAGCG GTACCGAACC 
AGTGGGGCAC CCCCTGAATC TAATCCCAGT GATGTGAAGG GCGAAGGGAC AAGAAAGAAC 
AATATGGAGA TCACGTGGAC GCCTATGAAT GCTACCTCTG CCTTTGGCCC CAACCTACGC 
TACATTGTCA AGTGGCGACG GAGAGAGACC CGAGAGACTT GGAACAATGT CACAGTGTGG 
GGCTCTCGCT ACGTGGTGGG GCAGACGCCT GTCTACGTTC CCTATGAGAT CCGAGTCCAG 
GCTGAAAATG ACTTTGGGAA AGGCCCCGAG CCTGACACCA TCATTGGGTA CTCCGGAGAA 
GATTATCCCA GGGCTGCGCC CACTGAAGTT AAAATCCGAG TCCTGAACAG CACAGCCATC 
AGCCTTCAGT GGAACCGAGT CTACTCTGAC ACGGTCCAGG GCCAGCTCAG AGAGTATCGA 
GCTTACTACT GGAGGGAAAG CAGTTTGCTG AAGAACCTGT GGGTGTCTCA GAAGAGACAG 
CAGGCCAGCT TCCCTGGTGA CCGTCCCCGG GGCGTGGTGG CCCGCCTGTT CCCCTACAGT 
AACTACAAGC TGGAGATGGT GGTGGTCAAT GGGAGAGGTG ACGGGCCTCG AAGTGAAACC 
AAGGAATTCA CCACCCCAGA AGGAGTACCC AGTGCCCCCA GGCGGTTCAG AGTCCGACAG 
CCCAACCTGG AGACCATCAA CCTGGAGTGG GACCACCCAG AGCACCCCAA CGGAATCCTG 
ATTGGATACA TCCTCAGATA CGTGCCCTTT AATGGAACCA AACTGGGAAA GCAGATGGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC TCTGTGCAGA GAGCAGACCC AGTGTCGCGT 
TACCGCTTCT CCCTCAGTGC CAGGACACAG GTGGGCTCTG GAGAAGCAGC CACAGAGGAG 
TCCCCAGCAC CTCCAAATGA AGCTTACACC AATAACCAGG CAGACATCGC CACCCAGGGC 
TGGTTCATCG GGCTCATGTG TGCCATTGCC CTTCTGGTGC TGATCCTTCT CATCGTCTGC 
TTCATCAAGA GGAGTCGAGG TGGCAAGTAC CCAGTGCGGG AAAAGAAGGA TGTCCCCTTG 
GGTCCTGAAG ACCCCAAAGA AGAAGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC ATCTCTGGAT GGCACCATCA AGCAGCAGGA GAGCGATGAC 
AGCCTGGTGG ACTATGGCGA AGGCGGCGAG GGCCAGTTCA ATGAAGATGG CTCCTTTATT 
GGCCAGTACA CTGTCAAAAA GGACAAGGAG GAAACGGAGG GCAATGAGAG CTCAGAGGCC 
ACATCACCAG TCAATGCCAT CTATTCCCTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006529653.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529716.2 Mus musculus neurofascin (Nfasc), transcript variant X20, mRNA.

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CloneID	OMu74381
Clone ID Related Accession (Same CDS sequence)	XM_006529653.4 , XM_006529653.3
Accession Version	XM_006529653.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529653.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCAGA AAGAACGCCC 
AGCTTCATGT ATCCCCAGGG CACATCGAGC AGTCAGATGG TTCTCCGTGG CATGGACCTG 
CTGCTGGAAT GCATTGCCTC TGGCGTCCCA ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTAACAA GGCCAAGTTC GAGAACTTTA ATAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCAT ACTGGCTGGA TGAGCCCAAG 
AACCTGATCC TGGCTCCTGG GGAAGATGGG AGGCTGGTAT GCCGAGCCAA TGGGAACCCG 
AAGCCGACCG TGCAGTGGAT GGTGAATGGA GAGCCTTTAC AATCGGCACC ACCCAATCCC 
AACCGTGAGG TAGCTGGAGA CACTATCATC TTCCGGGATA CTCAGATCAG CAGCAGGGCA 
GTGTACCAAT GTAATACATC CAATGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTGTTAGATG TACCCCCTCG GATGCTGTCT GCCCGCAACC AGCTCATCAG GGTGATCCTT 
TATAACCGGA CACGGCTGGA CTGTCCGTTC TTTGGGTCTC CCATCCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGCGGTAACT ACCACGTCTA CGAAAACGGC 
AGTCTAGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GCAAAGCCGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CCGAGGACCA GGTGGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAACATG ACCCCTCCTT GAAGCTCACA GTCTCCTGGC TGAAGGACGA TGAGCCACTC 
TACATTGGAA ACAGGATGAA GAAGGAAGAT GACTCCCTGA CGATCTTCGG AGTGGCAGAG 
CGGGACCAGG GCAGTTACAC GTGTATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGG GCGACCAGAC CGACCCAGGG ACCTGGAGCT CACTGACCTG 
GCTGAGAGGA GTGTGAGGCT GACCTGGATC CCAGGGGATG ACAACAACAG CCCTATCACA 
GACTACGTCG TTCAGTTTGA AGAGGACCAG TTCCAACCAG GGGTGTGGCA TGACCACTCC 
AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC CTCCATCTGT CCCCATATGT CAACTACCAA 
TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG AGCAGCCACC CCAGCCTTCC ATCCGAGCGG 
TACCGAACCA GTGGGGCACC CCCTGAATCT AATCCCAGTG ATGTGAAGGG CGAAGGGACA 
AGAAAGAACA ATATGGAGAT CACGTGGACG CCTATGAATG CTACCTCTGC CTTTGGCCCC 
AACCTACGCT ACATTGTCAA GTGGCGACGG AGAGAGACCC GAGAGACTTG GAACAATGTC 
ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG CAGACGCCTG TCTACGTTCC CTATGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAA GGCCCCGAGC CTGACACCAT CATTGGGTAC 
TCCGGAGAAG ATTATCCCAG GGCTGCGCCC ACTGAAGTTA AAATCCGAGT CCTGAACAGC 
ACAGCCATCA GCCTTCAGTG GAACCGAGTC TACTCTGACA CGGTCCAGGG CCAGCTCAGA 
GAGTATCGAG CTTACTACTG GAGGGAAAGC AGTTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGACAGC AGGCCAGCTT CCCTGGTGAC CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTG GTGGTCAATG GGAGAGGTGA CGGGCCTCGA 
AGTGAAACCA AGGAATTCAC CACCCCAGAA GGAGTACCCA GTGCCCCCAG GCGGTTCAGA 
GTCCGACAGC CCAACCTGGA GACCATCAAC CTGGAGTGGG ACCACCCAGA GCACCCCAAC 
GGAATCCTGA TTGGATACAT CCTCAGATAC GTGCCCTTTA ATGGAACCAA ACTGGGAAAG 
CAGATGGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCT CTGTGCAGAG AGCAGACCCA 
GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC AGGACACAGG TGGGCTCTGG AGAAGCAGCC 
ACAGAGGAGT CCCCAGCACC TCCAAATGAA GCTACTCCAA CTGCAGCTTA CACCAATAAC 
CAGGCAGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG 
GTGCTGATCC TTCTCATCGT CTGCTTCATC AAGAGGAGTC GAGGTGGCAA GTACCCAGTG 
CGGGAAAAGA AGGATGTCCC CTTGGGTCCT GAAGACCCCA AAGAAGAAGA TGGCTCATTT 
GACTACAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGATGGCACC 
ATCAAGCAGC AGGAGAGCGA TGACAGCCTG GTGGACTATG GCGAAGGCGG CGAGGGCCAG 
TTCAATGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA AAAAGGACAA GGAGGAAACG 
GAGGGCAATG AGAGCTCAGA GGCCACATCA CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529716.2
CDS	54..3833
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006529653.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529653.3

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCAGA AAGAACGCCC 
AGCTTCATGT ATCCCCAGGG CACATCGAGC AGTCAGATGG TTCTCCGTGG CATGGACCTG 
CTGCTGGAAT GCATTGCCTC TGGCGTCCCA ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTAACAA GGCCAAGTTC GAGAACTTTA ATAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCAT ACTGGCTGGA TGAGCCCAAG 
AACCTGATCC TGGCTCCTGG GGAAGATGGG AGGCTGGTAT GCCGAGCCAA TGGGAACCCG 
AAGCCGACCG TGCAGTGGAT GGTGAATGGA GAGCCTTTAC AATCGGCACC ACCCAATCCC 
AACCGTGAGG TAGCTGGAGA CACTATCATC TTCCGGGATA CTCAGATCAG CAGCAGGGCA 
GTGTACCAAT GTAATACATC CAATGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTGTTAGATG TACCCCCTCG GATGCTGTCT GCCCGCAACC AGCTCATCAG GGTGATCCTT 
TATAACCGGA CACGGCTGGA CTGTCCGTTC TTTGGGTCTC CCATCCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGCGGTAACT ACCACGTCTA CGAAAACGGC 
AGTCTAGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GCAAAGCCGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CCGAGGACCA GGTGGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAACATG ACCCCTCCTT GAAGCTCACA GTCTCCTGGC TGAAGGACGA TGAGCCACTC 
TACATTGGAA ACAGGATGAA GAAGGAAGAT GACTCCCTGA CGATCTTCGG AGTGGCAGAG 
CGGGACCAGG GCAGTTACAC GTGTATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGG GCGACCAGAC CGACCCAGGG ACCTGGAGCT CACTGACCTG 
GCTGAGAGGA GTGTGAGGCT GACCTGGATC CCAGGGGATG ACAACAACAG CCCTATCACA 
GACTACGTCG TTCAGTTTGA AGAGGACCAG TTCCAACCAG GGGTGTGGCA TGACCACTCC 
AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC CTCCATCTGT CCCCATATGT CAACTACCAA 
TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG AGCAGCCACC CCAGCCTTCC ATCCGAGCGG 
TACCGAACCA GTGGGGCACC CCCTGAATCT AATCCCAGTG ATGTGAAGGG CGAAGGGACA 
AGAAAGAACA ATATGGAGAT CACGTGGACG CCTATGAATG CTACCTCTGC CTTTGGCCCC 
AACCTACGCT ACATTGTCAA GTGGCGACGG AGAGAGACCC GAGAGACTTG GAACAATGTC 
ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG CAGACGCCTG TCTACGTTCC CTATGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAA GGCCCCGAGC CTGACACCAT CATTGGGTAC 
TCCGGAGAAG ATTATCCCAG GGCTGCGCCC ACTGAAGTTA AAATCCGAGT CCTGAACAGC 
ACAGCCATCA GCCTTCAGTG GAACCGAGTC TACTCTGACA CGGTCCAGGG CCAGCTCAGA 
GAGTATCGAG CTTACTACTG GAGGGAAAGC AGTTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGACAGC AGGCCAGCTT CCCTGGTGAC CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTG GTGGTCAATG GGAGAGGTGA CGGGCCTCGA 
AGTGAAACCA AGGAATTCAC CACCCCAGAA GGAGTACCCA GTGCCCCCAG GCGGTTCAGA 
GTCCGACAGC CCAACCTGGA GACCATCAAC CTGGAGTGGG ACCACCCAGA GCACCCCAAC 
GGAATCCTGA TTGGATACAT CCTCAGATAC GTGCCCTTTA ATGGAACCAA ACTGGGAAAG 
CAGATGGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCT CTGTGCAGAG AGCAGACCCA 
GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC AGGACACAGG TGGGCTCTGG AGAAGCAGCC 
ACAGAGGAGT CCCCAGCACC TCCAAATGAA GCTACTCCAA CTGCAGCTTA CACCAATAAC 
CAGGCAGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG 
GTGCTGATCC TTCTCATCGT CTGCTTCATC AAGAGGAGTC GAGGTGGCAA GTACCCAGTG 
CGGGAAAAGA AGGATGTCCC CTTGGGTCCT GAAGACCCCA AAGAAGAAGA TGGCTCATTT 
GACTACAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGATGGCACC 
ATCAAGCAGC AGGAGAGCGA TGACAGCCTG GTGGACTATG GCGAAGGCGG CGAGGGCCAG 
TTCAATGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA AAAAGGACAA GGAGGAAACG 
GAGGGCAATG AGAGCTCAGA GGCCACATCA CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248025.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246327.1 Mus musculus neurofascin (Nfasc), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OMu74387
Clone ID Related Accession (Same CDS sequence)	XM_011248025.3 , XM_011248025.2
Accession Version	XM_011248025.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248025.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAG TTAGACATAA AAGAGAGATA TCACAACAGA TTTCAATGAA GTCGAGAGAA 
TATGTAGGAC ATAGTTTAGT AAAACATGAT TTGGAAAGCC TAGAAGAAAC AGCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC GTTGCCCCCG 
ACTACTGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC 
AGTGAAGCCA CAACAGTTCC CATCATTCCA ACCGTCGTAC CTACCACCGT CGCCACCACC 
ATTGCCACAA CTACTACAAC CACTGCCGCC ACCACCACCA CCACCACTAC GGAGAGCCCT 
CCCACTACCA CTGCTGGGAC TAAGATTCAC GAAACCGCCC CCGACGAGCA GTCCATTTGG 
AACGTCACAG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCAGG 
CCTGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AGGCCCAGCC TATACAGCTG ACAGACCTCT TTCCCGGGAT GACGTACACG 
TTGCGGGTGT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC 
AGTACAGCTT ACACCAATAA CCAGGCAGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC 
ATGTGTGCCA TTGCCCTTCT GGTGCTGATC CTTCTCATCG TCTGCTTCAT CAAGAGGAGT 
CGAGGTGGCA AGTACCCAGT GCGGGAAAAG AAGGATGTCC CCTTGGGTCC TGAAGACCCC 
AAAGAAGAAG ATGGCTCATT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACATCTC TGGATGGCAC CATCAAGCAG CAGGAGAGCG ATGACAGCCT GGTGGACTAT 
GGCGAAGGCG GCGAGGGCCA GTTCAATGAA GATGGCTCCT TTATTGGCCA GTACACTGTC 
AAAAAGGACA AGGAGGAAAC GGAGGGCAAT GAGAGCTCAG AGGCCACATC ACCAGTCAAT 
GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246327.1
CDS	3101..6121
Translation	MNKVRHKREISQQISMKSREYVGHSLVKHDLESLEETAAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248025.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248025.2

ORF Insert Sequence:

ATGAATAAAG TTAGACATAA AAGAGAGATA TCACAACAGA TTTCAATGAA GTCGAGAGAA 
TATGTAGGAC ATAGTTTAGT AAAACATGAT TTGGAAAGCC TAGAAGAAAC AGCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC GTTGCCCCCG 
ACTACTGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC 
AGTGAAGCCA CAACAGTTCC CATCATTCCA ACCGTCGTAC CTACCACCGT CGCCACCACC 
ATTGCCACAA CTACTACAAC CACTGCCGCC ACCACCACCA CCACCACTAC GGAGAGCCCT 
CCCACTACCA CTGCTGGGAC TAAGATTCAC GAAACCGCCC CCGACGAGCA GTCCATTTGG 
AACGTCACAG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCAGG 
CCTGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AGGCCCAGCC TATACAGCTG ACAGACCTCT TTCCCGGGAT GACGTACACG 
TTGCGGGTGT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC 
AGTACAGCTT ACACCAATAA CCAGGCAGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC 
ATGTGTGCCA TTGCCCTTCT GGTGCTGATC CTTCTCATCG TCTGCTTCAT CAAGAGGAGT 
CGAGGTGGCA AGTACCCAGT GCGGGAAAAG AAGGATGTCC CCTTGGGTCC TGAAGACCCC 
AAAGAAGAAG ATGGCTCATT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACATCTC TGGATGGCAC CATCAAGCAG CAGGAGAGCG ATGACAGCCT GGTGGACTAT 
GGCGAAGGCG GCGAGGGCCA GTTCAATGAA GATGGCTCCT TTATTGGCCA GTACACTGTC 
AAAAAGGACA AGGAGGAAAC GGAGGGCAAT GAGAGCTCAG AGGCCACATC ACCAGTCAAT 
GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006529634.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529697.2 Mus musculus neurofascin (Nfasc), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu74366
Clone ID Related Accession (Same CDS sequence)	XM_006529634.3 , XM_006529634.2
Accession Version	XM_006529634.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006529634.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC GTTGCCCCCG 
ACTACTGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC 
AGTGAAGCCA CAACAGTTCC CATCATTCCA ACCGTCGTAC CTACCACCGT CGCCACCACC 
ATTGCCACAA CTACTACAAC CACTGCCGCC ACCACCACCA CCACCACTAC GGAGAGCCCT 
CCCACTACCA CTGCTGGGAC TAAGATTCAC GAAACCGCCC CCGACGAGCA GTCCATTTGG 
AACGTCACAG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCAGG 
CCTGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AGGCCCAGCC TATACAGCTG ACAGACCTCT TTCCCGGGAT GACGTACACG 
TTGCGGGTGT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC 
AGTACAGCTT ACACCAATAA CCAGGCAGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC 
ATGTGTGCCA TTGCCCTTCT GGTGCTGATC CTTCTCATCG TCTGCTTCAT CAAGAGGAGT 
CGAGGTGGCA AGTACCCAGT GCGGGAAAAG AAGGATGTCC CCTTGGGTCC TGAAGACCCC 
AAAGAAGAAG ATGGCTCATT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACATCTC TGGATGGCAC CATCAAGCAG CAGGAGAGCG ATGACAGCCT GGTGGACTAT 
GGCGAAGGCG GCGAGGGCCA GTTCAATGAA GATGGCTCCT TTATTGGCCA GTACACTGTC 
AAAAAGGACA AGGAGGAAAC GGAGGGCAAT GAGAGCTCAG AGGCCACATC ACCAGTCAAT 
GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529697.2
CDS	55..4455
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006529634.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529634.2

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC GTTGCCCCCG 
ACTACTGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC 
AGTGAAGCCA CAACAGTTCC CATCATTCCA ACCGTCGTAC CTACCACCGT CGCCACCACC 
ATTGCCACAA CTACTACAAC CACTGCCGCC ACCACCACCA CCACCACTAC GGAGAGCCCT 
CCCACTACCA CTGCTGGGAC TAAGATTCAC GAAACCGCCC CCGACGAGCA GTCCATTTGG 
AACGTCACAG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCAGG 
CCTGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AGGCCCAGCC TATACAGCTG ACAGACCTCT TTCCCGGGAT GACGTACACG 
TTGCGGGTGT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC 
AGTACAGCTT ACACCAATAA CCAGGCAGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC 
ATGTGTGCCA TTGCCCTTCT GGTGCTGATC CTTCTCATCG TCTGCTTCAT CAAGAGGAGT 
CGAGGTGGCA AGTACCCAGT GCGGGAAAAG AAGGATGTCC CCTTGGGTCC TGAAGACCCC 
AAAGAAGAAG ATGGCTCATT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACATCTC TGGATGGCAC CATCAAGCAG CAGGAGAGCG ATGACAGCCT GGTGGACTAT 
GGCGAAGGCG GCGAGGGCCA GTTCAATGAA GATGGCTCCT TTATTGGCCA GTACACTGTC 
AAAAAGGACA AGGAGGAAAC GGAGGGCAAT GAGAGCTCAG AGGCCACATC ACCAGTCAAT 
GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248011.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246313.1 Mus musculus neurofascin (Nfasc), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu74367
Clone ID Related Accession (Same CDS sequence)	XM_011248011.1 , XM_011248011.2
Accession Version	XM_011248011.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG CTGATCAGGC CACTCCAACT AACCGTTTGG CTGCCCTACC CAAAGGGCGA 
CCAGACCGAC CCAGGGACCT GGAGCTCACT GACCTGGCTG AGAGGAGTGT GAGGCTGACC 
TGGATCCCAG GGGATGACAA CAACAGCCCT ATCACAGACT ACGTCGTTCA GTTTGAAGAG 
GACCAGTTCC AACCAGGGGT GTGGCATGAC CACTCCAGGT TCCCAGGCAG CGTCAACTCA 
GCCGTCCTCC ATCTGTCCCC ATATGTCAAC TACCAATTCA GAGTCATCGC TGTCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTTCCATCC GAGCGGTACC GAACCAGTGG GGCACCCCCT 
GAATCTAATC CCAGTGATGT GAAGGGCGAA GGGACAAGAA AGAACAATAT GGAGATCACG 
TGGACGCCTA TGAATGCTAC CTCTGCCTTT GGCCCCAACC TACGCTACAT TGTCAAGTGG 
CGACGGAGAG AGACCCGAGA GACTTGGAAC AATGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CGCCTGTCTA CGTTCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTT 
GGGAAAGGCC CCGAGCCTGA CACCATCATT GGGTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAAT CCGAGTCCTG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGAGTCTACT CTGACACGGT CCAGGGCCAG CTCAGAGAGT ATCGAGCTTA CTACTGGAGG 
GAAAGCAGTT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAGGC CAGCTTCCCT 
GGTGACCGTC CCCGGGGCGT GGTGGCCCGC CTGTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTGGTGG TCAATGGGAG AGGTGACGGG CCTCGAAGTG AAACCAAGGA ATTCACCACC 
CCAGAAGGAG TACCCAGTGC CCCCAGGCGG TTCAGAGTCC GACAGCCCAA CCTGGAGACC 
ATCAACCTGG AGTGGGACCA CCCAGAGCAC CCCAACGGAA TCCTGATTGG ATACATCCTC 
AGATACGTGC CCTTTAATGG AACCAAACTG GGAAAGCAGA TGGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCTCTGT GCAGAGAGCA GACCCAGTGT CGCGTTACCG CTTCTCCCTC 
AGTGCCAGGA CACAGGTGGG CTCTGGAGAA GCAGCCACAG AGGAGTCCCC AGCACCTCCA 
AATGAAGCTA CTCCAACTGC AGCTCCTCCC ACGTTGCCCC CGACTACTGT GGGTACCACA 
GGCCTTGTGA GCAGTACTGA TGCTACTGCC CTTGCTGCCA CCAGTGAAGC CACAACAGTT 
CCCATCATTC CAACCGTCGT ACCTACCACC GTCGCCACCA CCATTGCCAC AACTACTACA 
ACCACTGCCG CCACCACCAC CACCACCACT ACGGAGAGCC CTCCCACTAC CACTGCTGGG 
ACTAAGATTC ACGAAACCGC CCCCGACGAG CAGTCCATTT GGAACGTCAC AGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCA GGCCTGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCTATACAGC TGACAGACCT CTTTCCCGGG ATGACGTACA CGTTGCGGGT GTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT 
AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT 
CTGGTGCTGA TCCTTCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAGTACCCA 
GTGCGGGAAA AGAAGGATGT CCCCTTGGGT CCTGAAGACC CCAAAGAAGA AGATGGCTCA 
TTTGACTACA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGATGGC 
ACCATCAAGC AGCAGGAGAG CGATGACAGC CTGGTGGACT ATGGCGAAGG CGGCGAGGGC 
CAGTTCAATG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAAAAAGGA CAAGGAGGAA 
ACGGAGGGCA ATGAGAGCTC AGAGGCCACA TCACCAGTCA ATGCCATCTA TTCCCTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246313.1
CDS	55..4437
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248011.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248011.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCAACAACC CCTATAATGA CTCGTCCTTA 
AGAAACCACC CTGACATATA TAGTGCCCGA GGAGTTGCAG AAAGAACGCC CAGCTTCATG 
TATCCCCAGG GCACATCGAG CAGTCAGATG GTTCTCCGTG GCATGGACCT GCTGCTGGAA 
TGCATTGCCT CTGGCGTCCC AACACCAGAC ATTGCATGGT ACAAGAAAGG TGGGGACCTC 
CCATCTAACA AGGCCAAGTT CGAGAACTTT AATAAGGCTC TGCGCATCAC CAATGTCTCT 
GAAGAGGACT CTGGGGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC 
ACGATCTCGG TGAGAGTAAA GGCTGCTCCA TACTGGCTGG ATGAGCCCAA GAACCTGATC 
CTGGCTCCTG GGGAAGATGG GAGGCTGGTA TGCCGAGCCA ATGGGAACCC GAAGCCGACC 
GTGCAGTGGA TGGTGAATGG AGAGCCTTTA CAATCGGCAC CACCCAATCC CAACCGTGAG 
GTAGCTGGAG ACACTATCAT CTTCCGGGAT ACTCAGATCA GCAGCAGGGC AGTGTACCAA 
TGTAATACAT CCAATGAACA TGGCTACCTG CTGGCCAATG CCTTCGTCAG CGTGTTAGAT 
GTACCCCCTC GGATGCTGTC TGCCCGCAAC CAGCTCATCA GGGTGATCCT TTATAACCGG 
ACACGGCTGG ACTGTCCGTT CTTTGGGTCT CCCATCCCAA CACTCCGATG GTTTAAGAAT 
GGGCAAGGAA GCAACCTGGA TGGCGGTAAC TACCACGTCT ACGAAAACGG CAGTCTAGAA 
ATCAAGATGA TTCGCAAAGA GGACCAAGGC ATCTACACCT GTGTGGCCAC CAACATCCTG 
GGCAAAGCCG AAAATCAAGT CCGCCTGGAG GTCAAAGACC CCACCAGGAT CTACAGGATG 
CCCGAGGACC AGGTGGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG CGTGAAACAT 
GACCCCTCCT TGAAGCTCAC AGTCTCCTGG CTGAAGGACG ATGAGCCACT CTACATTGGA 
AACAGGATGA AGAAGGAAGA TGACTCCCTG ACGATCTTCG GAGTGGCAGA GCGGGACCAG 
GGCAGTTACA CGTGTATGGC CAGCACCGAG CTGGACCAGG ACCTGGCAAA GGCCTACCTC 
ACTGTTCTAG CTGATCAGGC CACTCCAACT AACCGTTTGG CTGCCCTACC CAAAGGGCGA 
CCAGACCGAC CCAGGGACCT GGAGCTCACT GACCTGGCTG AGAGGAGTGT GAGGCTGACC 
TGGATCCCAG GGGATGACAA CAACAGCCCT ATCACAGACT ACGTCGTTCA GTTTGAAGAG 
GACCAGTTCC AACCAGGGGT GTGGCATGAC CACTCCAGGT TCCCAGGCAG CGTCAACTCA 
GCCGTCCTCC ATCTGTCCCC ATATGTCAAC TACCAATTCA GAGTCATCGC TGTCAACGAG 
GTTGGGAGCA GCCACCCCAG CCTTCCATCC GAGCGGTACC GAACCAGTGG GGCACCCCCT 
GAATCTAATC CCAGTGATGT GAAGGGCGAA GGGACAAGAA AGAACAATAT GGAGATCACG 
TGGACGCCTA TGAATGCTAC CTCTGCCTTT GGCCCCAACC TACGCTACAT TGTCAAGTGG 
CGACGGAGAG AGACCCGAGA GACTTGGAAC AATGTCACAG TGTGGGGCTC TCGCTACGTG 
GTGGGGCAGA CGCCTGTCTA CGTTCCCTAT GAGATCCGAG TCCAGGCTGA AAATGACTTT 
GGGAAAGGCC CCGAGCCTGA CACCATCATT GGGTACTCCG GAGAAGATTA TCCCAGGGCT 
GCGCCCACTG AAGTTAAAAT CCGAGTCCTG AACAGCACAG CCATCAGCCT TCAGTGGAAC 
CGAGTCTACT CTGACACGGT CCAGGGCCAG CTCAGAGAGT ATCGAGCTTA CTACTGGAGG 
GAAAGCAGTT TGCTGAAGAA CCTGTGGGTG TCTCAGAAGA GACAGCAGGC CAGCTTCCCT 
GGTGACCGTC CCCGGGGCGT GGTGGCCCGC CTGTTCCCCT ACAGTAACTA CAAGCTGGAG 
ATGGTGGTGG TCAATGGGAG AGGTGACGGG CCTCGAAGTG AAACCAAGGA ATTCACCACC 
CCAGAAGGAG TACCCAGTGC CCCCAGGCGG TTCAGAGTCC GACAGCCCAA CCTGGAGACC 
ATCAACCTGG AGTGGGACCA CCCAGAGCAC CCCAACGGAA TCCTGATTGG ATACATCCTC 
AGATACGTGC CCTTTAATGG AACCAAACTG GGAAAGCAGA TGGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCTCTGT GCAGAGAGCA GACCCAGTGT CGCGTTACCG CTTCTCCCTC 
AGTGCCAGGA CACAGGTGGG CTCTGGAGAA GCAGCCACAG AGGAGTCCCC AGCACCTCCA 
AATGAAGCTA CTCCAACTGC AGCTCCTCCC ACGTTGCCCC CGACTACTGT GGGTACCACA 
GGCCTTGTGA GCAGTACTGA TGCTACTGCC CTTGCTGCCA CCAGTGAAGC CACAACAGTT 
CCCATCATTC CAACCGTCGT ACCTACCACC GTCGCCACCA CCATTGCCAC AACTACTACA 
ACCACTGCCG CCACCACCAC CACCACCACT ACGGAGAGCC CTCCCACTAC CACTGCTGGG 
ACTAAGATTC ACGAAACCGC CCCCGACGAG CAGTCCATTT GGAACGTCAC AGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCA GGCCTGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGGC CCAGGCCCAG 
CCTATACAGC TGACAGACCT CTTTCCCGGG ATGACGTACA CGTTGCGGGT GTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAT 
AACCAGGCAG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT 
CTGGTGCTGA TCCTTCTCAT CGTCTGCTTC ATCAAGAGGA GTCGAGGTGG CAAGTACCCA 
GTGCGGGAAA AGAAGGATGT CCCCTTGGGT CCTGAAGACC CCAAAGAAGA AGATGGCTCA 
TTTGACTACA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGATGGC 
ACCATCAAGC AGCAGGAGAG CGATGACAGC CTGGTGGACT ATGGCGAAGG CGGCGAGGGC 
CAGTTCAATG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAAAAAGGA CAAGGAGGAA 
ACGGAGGGCA ATGAGAGCTC AGAGGCCACA TCACCAGTCA ATGCCATCTA TTCCCTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248012.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246314.1 Mus musculus neurofascin (Nfasc), transcript variant X3, mRNA.

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CloneID	OMu74368
Clone ID Related Accession (Same CDS sequence)	XM_011248012.2 , XM_011248012.1
Accession Version	XM_011248012.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGGG CGACCAGACC GACCCAGGGA CCTGGAGCTC 
ACTGACCTGG CTGAGAGGAG TGTGAGGCTG ACCTGGATCC CAGGGGATGA CAACAACAGC 
CCTATCACAG ACTACGTCGT TCAGTTTGAA GAGGACCAGT TCCAACCAGG GGTGTGGCAT 
GACCACTCCA GGTTCCCAGG CAGCGTCAAC TCAGCCGTCC TCCATCTGTC CCCATATGTC 
AACTACCAAT TCAGAGTCAT CGCTGTCAAC GAGGTTGGGA GCAGCCACCC CAGCCTTCCA 
TCCGAGCGGT ACCGAACCAG TGGGGCACCC CCTGAATCTA ATCCCAGTGA TGTGAAGGGC 
GAAGGGACAA GAAAGAACAA TATGGAGATC ACGTGGACGC CTATGAATGC TACCTCTGCC 
TTTGGCCCCA ACCTACGCTA CATTGTCAAG TGGCGACGGA GAGAGACCCG AGAGACTTGG 
AACAATGTCA CAGTGTGGGG CTCTCGCTAC GTGGTGGGGC AGACGCCTGT CTACGTTCCC 
TATGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAAG GCCCCGAGCC TGACACCATC 
ATTGGGTACT CCGGAGAAGA TTATCCCAGG GCTGCGCCCA CTGAAGTTAA AATCCGAGTC 
CTGAACAGCA CAGCCATCAG CCTTCAGTGG AACCGAGTCT ACTCTGACAC GGTCCAGGGC 
CAGCTCAGAG AGTATCGAGC TTACTACTGG AGGGAAAGCA GTTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGACAGCA GGCCAGCTTC CCTGGTGACC GTCCCCGGGG CGTGGTGGCC 
CGCCTGTTCC CCTACAGTAA CTACAAGCTG GAGATGGTGG TGGTCAATGG GAGAGGTGAC 
GGGCCTCGAA GTGAAACCAA GGAATTCACC ACCCCAGAAG GAGTACCCAG TGCCCCCAGG 
CGGTTCAGAG TCCGACAGCC CAACCTGGAG ACCATCAACC TGGAGTGGGA CCACCCAGAG 
CACCCCAACG GAATCCTGAT TGGATACATC CTCAGATACG TGCCCTTTAA TGGAACCAAA 
CTGGGAAAGC AGATGGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCTC TGTGCAGAGA 
GCAGACCCAG TGTCGCGTTA CCGCTTCTCC CTCAGTGCCA GGACACAGGT GGGCTCTGGA 
GAAGCAGCCA CAGAGGAGTC CCCAGCACCT CCAAATGAAG CTACTCCAAC TGCAGCTCCT 
CCCACGTTGC CCCCGACTAC TGTGGGTACC ACAGGCCTTG TGAGCAGTAC TGATGCTACT 
GCCCTTGCTG CCACCAGTGA AGCCACAACA GTTCCCATCA TTCCAACCGT CGTACCTACC 
ACCGTCGCCA CCACCATTGC CACAACTACT ACAACCACTG CCGCCACCAC CACCACCACC 
ACTACGGAGA GCCCTCCCAC TACCACTGCT GGGACTAAGA TTCACGAAAC CGCCCCCGAC 
GAGCAGTCCA TTTGGAACGT CACAGTGCTC CCCAACAGTA AATGGGCCAA CATCACCTGG 
AAGCACAATT TCAGGCCTGG AACTGACTTT GTGGTTGAGT ACATCGACAG CAACCATACG 
AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCTATAC AGCTGACAGA CCTCTTTCCC 
GGGATGACGT ACACGTTGCG GGTGTATTCC CGGGACAACG AGGGCATCAG CAGTACCGTC 
ATCACCTTTA TGACCAGTAC AGCTTACACC AATAACCAGG CAGACATCGC CACCCAGGGC 
TGGTTCATCG GGCTCATGTG TGCCATTGCC CTTCTGGTGC TGATCCTTCT CATCGTCTGC 
TTCATCAAGA GGAGTCGAGG TGGCAAGTAC CCAGTGCGGG AAAAGAAGGA TGTCCCCTTG 
GGTCCTGAAG ACCCCAAAGA AGAAGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC ATCTCTGGAT GGCACCATCA AGCAGCAGGA GAGCGATGAC 
AGCCTGGTGG ACTATGGCGA AGGCGGCGAG GGCCAGTTCA ATGAAGATGG CTCCTTTATT 
GGCCAGTACA CTGTCAAAAA GGACAAGGAG GAAACGGAGG GCAATGAGAG CTCAGAGGCC 
ACATCACCAG TCAATGCCAT CTATTCCCTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246314.1
CDS	55..4410
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248012.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248012.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGGG CGACCAGACC GACCCAGGGA CCTGGAGCTC 
ACTGACCTGG CTGAGAGGAG TGTGAGGCTG ACCTGGATCC CAGGGGATGA CAACAACAGC 
CCTATCACAG ACTACGTCGT TCAGTTTGAA GAGGACCAGT TCCAACCAGG GGTGTGGCAT 
GACCACTCCA GGTTCCCAGG CAGCGTCAAC TCAGCCGTCC TCCATCTGTC CCCATATGTC 
AACTACCAAT TCAGAGTCAT CGCTGTCAAC GAGGTTGGGA GCAGCCACCC CAGCCTTCCA 
TCCGAGCGGT ACCGAACCAG TGGGGCACCC CCTGAATCTA ATCCCAGTGA TGTGAAGGGC 
GAAGGGACAA GAAAGAACAA TATGGAGATC ACGTGGACGC CTATGAATGC TACCTCTGCC 
TTTGGCCCCA ACCTACGCTA CATTGTCAAG TGGCGACGGA GAGAGACCCG AGAGACTTGG 
AACAATGTCA CAGTGTGGGG CTCTCGCTAC GTGGTGGGGC AGACGCCTGT CTACGTTCCC 
TATGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAAG GCCCCGAGCC TGACACCATC 
ATTGGGTACT CCGGAGAAGA TTATCCCAGG GCTGCGCCCA CTGAAGTTAA AATCCGAGTC 
CTGAACAGCA CAGCCATCAG CCTTCAGTGG AACCGAGTCT ACTCTGACAC GGTCCAGGGC 
CAGCTCAGAG AGTATCGAGC TTACTACTGG AGGGAAAGCA GTTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGACAGCA GGCCAGCTTC CCTGGTGACC GTCCCCGGGG CGTGGTGGCC 
CGCCTGTTCC CCTACAGTAA CTACAAGCTG GAGATGGTGG TGGTCAATGG GAGAGGTGAC 
GGGCCTCGAA GTGAAACCAA GGAATTCACC ACCCCAGAAG GAGTACCCAG TGCCCCCAGG 
CGGTTCAGAG TCCGACAGCC CAACCTGGAG ACCATCAACC TGGAGTGGGA CCACCCAGAG 
CACCCCAACG GAATCCTGAT TGGATACATC CTCAGATACG TGCCCTTTAA TGGAACCAAA 
CTGGGAAAGC AGATGGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCTC TGTGCAGAGA 
GCAGACCCAG TGTCGCGTTA CCGCTTCTCC CTCAGTGCCA GGACACAGGT GGGCTCTGGA 
GAAGCAGCCA CAGAGGAGTC CCCAGCACCT CCAAATGAAG CTACTCCAAC TGCAGCTCCT 
CCCACGTTGC CCCCGACTAC TGTGGGTACC ACAGGCCTTG TGAGCAGTAC TGATGCTACT 
GCCCTTGCTG CCACCAGTGA AGCCACAACA GTTCCCATCA TTCCAACCGT CGTACCTACC 
ACCGTCGCCA CCACCATTGC CACAACTACT ACAACCACTG CCGCCACCAC CACCACCACC 
ACTACGGAGA GCCCTCCCAC TACCACTGCT GGGACTAAGA TTCACGAAAC CGCCCCCGAC 
GAGCAGTCCA TTTGGAACGT CACAGTGCTC CCCAACAGTA AATGGGCCAA CATCACCTGG 
AAGCACAATT TCAGGCCTGG AACTGACTTT GTGGTTGAGT ACATCGACAG CAACCATACG 
AAAAAAACTG TCCCTGTTAA GGCCCAGGCC CAGCCTATAC AGCTGACAGA CCTCTTTCCC 
GGGATGACGT ACACGTTGCG GGTGTATTCC CGGGACAACG AGGGCATCAG CAGTACCGTC 
ATCACCTTTA TGACCAGTAC AGCTTACACC AATAACCAGG CAGACATCGC CACCCAGGGC 
TGGTTCATCG GGCTCATGTG TGCCATTGCC CTTCTGGTGC TGATCCTTCT CATCGTCTGC 
TTCATCAAGA GGAGTCGAGG TGGCAAGTAC CCAGTGCGGG AAAAGAAGGA TGTCCCCTTG 
GGTCCTGAAG ACCCCAAAGA AGAAGATGGC TCATTTGACT ACAGTGATGA GGACAACAAG 
CCCCTGCAGG GCAGCCAGAC ATCTCTGGAT GGCACCATCA AGCAGCAGGA GAGCGATGAC 
AGCCTGGTGG ACTATGGCGA AGGCGGCGAG GGCCAGTTCA ATGAAGATGG CTCCTTTATT 
GGCCAGTACA CTGTCAAAAA GGACAAGGAG GAAACGGAGG GCAATGAGAG CTCAGAGGCC 
ACATCACCAG TCAATGCCAT CTATTCCCTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006529635.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529698.2 Mus musculus neurofascin (Nfasc), transcript variant X4, mRNA.

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CloneID	OMu74369
Clone ID Related Accession (Same CDS sequence)	XM_006529635.4 , XM_006529635.3
Accession Version	XM_006529635.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529635.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CACCCGAGGA 
GTTGCAGAAA GAACGCCCAG CTTCATGTAT CCCCAGGGCA CATCGAGCAG TCAGATGGTT 
CTCCGTGGCA TGGACCTGCT GCTGGAATGC ATTGCCTCTG GCGTCCCAAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTAACAAGG CCAAGTTCGA GAACTTTAAT 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCTCCATAC 
TGGCTGGATG AGCCCAAGAA CCTGATCCTG GCTCCTGGGG AAGATGGGAG GCTGGTATGC 
CGAGCCAATG GGAACCCGAA GCCGACCGTG CAGTGGATGG TGAATGGAGA GCCTTTACAA 
TCGGCACCAC CCAATCCCAA CCGTGAGGTA GCTGGAGACA CTATCATCTT CCGGGATACT 
CAGATCAGCA GCAGGGCAGT GTACCAATGT AATACATCCA ATGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT GTTAGATGTA CCCCCTCGGA TGCTGTCTGC CCGCAACCAG 
CTCATCAGGG TGATCCTTTA TAACCGGACA CGGCTGGACT GTCCGTTCTT TGGGTCTCCC 
ATCCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG CGGTAACTAC 
CACGTCTACG AAAACGGCAG TCTAGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGC AAAGCCGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCC GAGGACCAGG TGGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAACATGAC CCCTCCTTGA AGCTCACAGT CTCCTGGCTG 
AAGGACGATG AGCCACTCTA CATTGGAAAC AGGATGAAGA AGGAAGATGA CTCCCTGACG 
ATCTTCGGAG TGGCAGAGCG GGACCAGGGC AGTTACACGT GTATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTACCCAA AGGGCGACCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCTGAGA GGAGTGTGAG GCTGACCTGG ATCCCAGGGG ATGACAACAA CAGCCCTATC 
ACAGACTACG TCGTTCAGTT TGAAGAGGAC CAGTTCCAAC CAGGGGTGTG GCATGACCAC 
TCCAGGTTCC CAGGCAGCGT CAACTCAGCC GTCCTCCATC TGTCCCCATA TGTCAACTAC 
CAATTCAGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CCAGTGGGGC ACCCCCTGAA TCTAATCCCA GTGATGTGAA GGGCGAAGGG 
ACAAGAAAGA ACAATATGGA GATCACGTGG ACGCCTATGA ATGCTACCTC TGCCTTTGGC 
CCCAACCTAC GCTACATTGT CAAGTGGCGA CGGAGAGAGA CCCGAGAGAC TTGGAACAAT 
GTCACAGTGT GGGGCTCTCG CTACGTGGTG GGGCAGACGC CTGTCTACGT TCCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCCG AGCCTGACAC CATCATTGGG 
TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGA GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGT GACCGTCCCC GGGGCGTGGT GGCCCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTGGTGGTCA ATGGGAGAGG TGACGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCA GAAGGAGTAC CCAGTGCCCC CAGGCGGTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAGT GGGACCACCC AGAGCACCCC 
AACGGAATCC TGATTGGATA CATCCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCTGTGCA GAGAGCAGAC 
CCAGTGTCGC GTTACCGCTT CTCCCTCAGT GCCAGGACAC AGGTGGGCTC TGGAGAAGCA 
GCCACAGAGG AGTCCCCAGC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACG 
TTGCCCCCGA CTACTGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT 
GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATTCCAA CCGTCGTACC TACCACCGTC 
GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCA CCACCACCAC CACCACTACG 
GAGAGCCCTC CCACTACCAC TGCTGGGACT AAGATTCACG AAACCGCCCC CGACGAGCAG 
TCCATTTGGA ACGTCACAGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC 
AATTTCAGGC CTGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA 
ACTGTCCCTG TTAAGGCCCA GGCCCAGCCT ATACAGCTGA CAGACCTCTT TCCCGGGATG 
ACGTACACGT TGCGGGTGTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC 
TTTATGACCA GTACAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TTCTCATCGT CTGCTTCATC 
AAGAGGAGTC GAGGTGGCAA GTACCCAGTG CGGGAAAAGA AGGATGTCCC CTTGGGTCCT 
GAAGACCCCA AAGAAGAAGA TGGCTCATTT GACTACAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGATGGCACC ATCAAGCAGC AGGAGAGCGA TGACAGCCTG 
GTGGACTATG GCGAAGGCGG CGAGGGCCAG TTCAATGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA AAAAGGACAA GGAGGAAACG GAGGGCAATG AGAGCTCAGA GGCCACATCA 
CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529698.2
CDS	54..4403
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006529635.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529635.3

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CACCCGAGGA 
GTTGCAGAAA GAACGCCCAG CTTCATGTAT CCCCAGGGCA CATCGAGCAG TCAGATGGTT 
CTCCGTGGCA TGGACCTGCT GCTGGAATGC ATTGCCTCTG GCGTCCCAAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTAACAAGG CCAAGTTCGA GAACTTTAAT 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCTCCATAC 
TGGCTGGATG AGCCCAAGAA CCTGATCCTG GCTCCTGGGG AAGATGGGAG GCTGGTATGC 
CGAGCCAATG GGAACCCGAA GCCGACCGTG CAGTGGATGG TGAATGGAGA GCCTTTACAA 
TCGGCACCAC CCAATCCCAA CCGTGAGGTA GCTGGAGACA CTATCATCTT CCGGGATACT 
CAGATCAGCA GCAGGGCAGT GTACCAATGT AATACATCCA ATGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT GTTAGATGTA CCCCCTCGGA TGCTGTCTGC CCGCAACCAG 
CTCATCAGGG TGATCCTTTA TAACCGGACA CGGCTGGACT GTCCGTTCTT TGGGTCTCCC 
ATCCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG CGGTAACTAC 
CACGTCTACG AAAACGGCAG TCTAGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGC AAAGCCGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCC GAGGACCAGG TGGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAACATGAC CCCTCCTTGA AGCTCACAGT CTCCTGGCTG 
AAGGACGATG AGCCACTCTA CATTGGAAAC AGGATGAAGA AGGAAGATGA CTCCCTGACG 
ATCTTCGGAG TGGCAGAGCG GGACCAGGGC AGTTACACGT GTATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTACCCAA AGGGCGACCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCTGAGA GGAGTGTGAG GCTGACCTGG ATCCCAGGGG ATGACAACAA CAGCCCTATC 
ACAGACTACG TCGTTCAGTT TGAAGAGGAC CAGTTCCAAC CAGGGGTGTG GCATGACCAC 
TCCAGGTTCC CAGGCAGCGT CAACTCAGCC GTCCTCCATC TGTCCCCATA TGTCAACTAC 
CAATTCAGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CCAGTGGGGC ACCCCCTGAA TCTAATCCCA GTGATGTGAA GGGCGAAGGG 
ACAAGAAAGA ACAATATGGA GATCACGTGG ACGCCTATGA ATGCTACCTC TGCCTTTGGC 
CCCAACCTAC GCTACATTGT CAAGTGGCGA CGGAGAGAGA CCCGAGAGAC TTGGAACAAT 
GTCACAGTGT GGGGCTCTCG CTACGTGGTG GGGCAGACGC CTGTCTACGT TCCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCCG AGCCTGACAC CATCATTGGG 
TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGA GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGT GACCGTCCCC GGGGCGTGGT GGCCCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTGGTGGTCA ATGGGAGAGG TGACGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCA GAAGGAGTAC CCAGTGCCCC CAGGCGGTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAGT GGGACCACCC AGAGCACCCC 
AACGGAATCC TGATTGGATA CATCCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCTGTGCA GAGAGCAGAC 
CCAGTGTCGC GTTACCGCTT CTCCCTCAGT GCCAGGACAC AGGTGGGCTC TGGAGAAGCA 
GCCACAGAGG AGTCCCCAGC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACG 
TTGCCCCCGA CTACTGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT 
GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATTCCAA CCGTCGTACC TACCACCGTC 
GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCA CCACCACCAC CACCACTACG 
GAGAGCCCTC CCACTACCAC TGCTGGGACT AAGATTCACG AAACCGCCCC CGACGAGCAG 
TCCATTTGGA ACGTCACAGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC 
AATTTCAGGC CTGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA 
ACTGTCCCTG TTAAGGCCCA GGCCCAGCCT ATACAGCTGA CAGACCTCTT TCCCGGGATG 
ACGTACACGT TGCGGGTGTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC 
TTTATGACCA GTACAGCTTA CACCAATAAC CAGGCAGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TTCTCATCGT CTGCTTCATC 
AAGAGGAGTC GAGGTGGCAA GTACCCAGTG CGGGAAAAGA AGGATGTCCC CTTGGGTCCT 
GAAGACCCCA AAGAAGAAGA TGGCTCATTT GACTACAGTG ATGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGATGGCACC ATCAAGCAGC AGGAGAGCGA TGACAGCCTG 
GTGGACTATG GCGAAGGCGG CGAGGGCCAG TTCAATGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA AAAAGGACAA GGAGGAAACG GAGGGCAATG AGAGCTCAGA GGCCACATCA 
CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248013.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246315.1 Mus musculus neurofascin (Nfasc), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu74370
Clone ID Related Accession (Same CDS sequence)	XM_011248013.3 , XM_011248013.2
Accession Version	XM_011248013.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCAGA AAGAACGCCC 
AGCTTCATGT ATCCCCAGGG CACATCGAGC AGTCAGATGG TTCTCCGTGG CATGGACCTG 
CTGCTGGAAT GCATTGCCTC TGGCGTCCCA ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTAACAA GGCCAAGTTC GAGAACTTTA ATAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCAT ACTGGCTGGA TGAGCCCAAG 
AACCTGATCC TGGCTCCTGG GGAAGATGGG AGGCTGGTAT GCCGAGCCAA TGGGAACCCG 
AAGCCGACCG TGCAGTGGAT GGTGAATGGA GAGCCTTTAC AATCGGCACC ACCCAATCCC 
AACCGTGAGG TAGCTGGAGA CACTATCATC TTCCGGGATA CTCAGATCAG CAGCAGGGCA 
GTGTACCAAT GTAATACATC CAATGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTGTTAGATG TACCCCCTCG GATGCTGTCT GCCCGCAACC AGCTCATCAG GGTGATCCTT 
TATAACCGGA CACGGCTGGA CTGTCCGTTC TTTGGGTCTC CCATCCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGCGGTAACT ACCACGTCTA CGAAAACGGC 
AGTCTAGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GCAAAGCCGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CCGAGGACCA GGTGGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAACATG ACCCCTCCTT GAAGCTCACA GTCTCCTGGC TGAAGGACGA TGAGCCACTC 
TACATTGGAA ACAGGATGAA GAAGGAAGAT GACTCCCTGA CGATCTTCGG AGTGGCAGAG 
CGGGACCAGG GCAGTTACAC GTGTATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTACCC 
AAAGGGCGAC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG 
AGGCTGACCT GGATCCCAGG GGATGACAAC AACAGCCCTA TCACAGACTA CGTCGTTCAG 
TTTGAAGAGG ACCAGTTCCA ACCAGGGGTG TGGCATGACC ACTCCAGGTT CCCAGGCAGC 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCA TATGTCAACT ACCAATTCAG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACCAGTGGG 
GCACCCCCTG AATCTAATCC CAGTGATGTG AAGGGCGAAG GGACAAGAAA GAACAATATG 
GAGATCACGT GGACGCCTAT GAATGCTACC TCTGCCTTTG GCCCCAACCT ACGCTACATT 
GTCAAGTGGC GACGGAGAGA GACCCGAGAG ACTTGGAACA ATGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC GCCTGTCTAC GTTCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC CGAGCCTGAC ACCATCATTG GGTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GAGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GTGACCGTCC CCGGGGCGTG GTGGCCCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTGGTGGT CAATGGGAGA GGTGACGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGGT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA GTGGGACCAC CCAGAGCACC CCAACGGAAT CCTGATTGGA 
TACATCCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCTGTG CAGAGAGCAG ACCCAGTGTC GCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC ACAGGTGGGC TCTGGAGAAG CAGCCACAGA GGAGTCCCCA 
GCACCTCCAA ATGAAGCTAC TCCAACTGCA GCTCCTCCCA CGTTGCCCCC GACTACTGTG 
GGTACCACAG GCCTTGTGAG CAGTACTGAT GCTACTGCCC TTGCTGCCAC CAGTGAAGCC 
ACAACAGTTC CCATCATTCC AACCGTCGTA CCTACCACCG TCGCCACCAC CATTGCCACA 
ACTACTACAA CCACTGCCGC CACCACCACC ACCACCACTA CGGAGAGCCC TCCCACTACC 
ACTGCTGGGA CTAAGATTCA CGAAACCGCC CCCGACGAGC AGTCCATTTG GAACGTCACA 
GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCAG GCCTGGAACT 
GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC TGTTAAGGCC 
CAGGCCCAGC CTATACAGCT GACAGACCTC TTTCCCGGGA TGACGTACAC GTTGCGGGTG 
TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT 
TACACCAATA ACCAGGCAGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGTGCC 
ATTGCCCTTC TGGTGCTGAT CCTTCTCATC GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC 
AAGTACCCAG TGCGGGAAAA GAAGGATGTC CCCTTGGGTC CTGAAGACCC CAAAGAAGAA 
GATGGCTCAT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCT 
CTGGATGGCA CCATCAAGCA GCAGGAGAGC GATGACAGCC TGGTGGACTA TGGCGAAGGC 
GGCGAGGGCC AGTTCAATGA AGATGGCTCC TTTATTGGCC AGTACACTGT CAAAAAGGAC 
AAGGAGGAAA CGGAGGGCAA TGAGAGCTCA GAGGCCACAT CACCAGTCAA TGCCATCTAT 
TCCCTTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246315.1
CDS	54..4385
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248013.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248013.2

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCTGA CCCAACCCCC AACTATCACC 
AAGCAGTCCG TGAAGGACCA CATCGTGGAC CCTCGAGATA ACATCCTGAT TGAATGTGAA 
GCTAAAGGCA ACCCCGCCCC CAGTTTTCAC TGGACTCGCA ACAGCAGATT CTTCAACATT 
GCCAAGGACC CACGGGTGTC CATGAGGAGG AGATCTGGGA CCTTGGTGAT CGACTTCCGC 
AGTGGTGGGC GGCCTGAGGA ATACGAAGGG GAGTACCAGT GCTTTGCCCG GAACAAATTT 
GGCACTGCAC TTAGCAACCG CATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG 
GAAAACCTAG ACCCCGTCGT GGTTCAAGAG GGTGCCCCCT TGACACTACA GTGCAACCCC 
CCACCCGGCC TCCCGTCCCC CGTCATCTTC TGGATGAGCA GCTCCATGGA GCCCATCACC 
CAGGACAAGC GTGTCTCCCA GGGTCACAAC GGGGACCTGT ACTTCTCCAA CGTCATGCTG 
CAGGACATGC AGACCGACTA CAGCTGCAAC GCGCGCTTTC ACTTCACCCA CACCATTCAG 
CAGAAGAACC CCTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCAGA AAGAACGCCC 
AGCTTCATGT ATCCCCAGGG CACATCGAGC AGTCAGATGG TTCTCCGTGG CATGGACCTG 
CTGCTGGAAT GCATTGCCTC TGGCGTCCCA ACACCAGACA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTAACAA GGCCAAGTTC GAGAACTTTA ATAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCAT ACTGGCTGGA TGAGCCCAAG 
AACCTGATCC TGGCTCCTGG GGAAGATGGG AGGCTGGTAT GCCGAGCCAA TGGGAACCCG 
AAGCCGACCG TGCAGTGGAT GGTGAATGGA GAGCCTTTAC AATCGGCACC ACCCAATCCC 
AACCGTGAGG TAGCTGGAGA CACTATCATC TTCCGGGATA CTCAGATCAG CAGCAGGGCA 
GTGTACCAAT GTAATACATC CAATGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTGTTAGATG TACCCCCTCG GATGCTGTCT GCCCGCAACC AGCTCATCAG GGTGATCCTT 
TATAACCGGA CACGGCTGGA CTGTCCGTTC TTTGGGTCTC CCATCCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGCGGTAACT ACCACGTCTA CGAAAACGGC 
AGTCTAGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GCAAAGCCGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CCGAGGACCA GGTGGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGC 
GTGAAACATG ACCCCTCCTT GAAGCTCACA GTCTCCTGGC TGAAGGACGA TGAGCCACTC 
TACATTGGAA ACAGGATGAA GAAGGAAGAT GACTCCCTGA CGATCTTCGG AGTGGCAGAG 
CGGGACCAGG GCAGTTACAC GTGTATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGC TGATCAGGCC ACTCCAACTA ACCGTTTGGC TGCCCTACCC 
AAAGGGCGAC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG 
AGGCTGACCT GGATCCCAGG GGATGACAAC AACAGCCCTA TCACAGACTA CGTCGTTCAG 
TTTGAAGAGG ACCAGTTCCA ACCAGGGGTG TGGCATGACC ACTCCAGGTT CCCAGGCAGC 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCA TATGTCAACT ACCAATTCAG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACCAGTGGG 
GCACCCCCTG AATCTAATCC CAGTGATGTG AAGGGCGAAG GGACAAGAAA GAACAATATG 
GAGATCACGT GGACGCCTAT GAATGCTACC TCTGCCTTTG GCCCCAACCT ACGCTACATT 
GTCAAGTGGC GACGGAGAGA GACCCGAGAG ACTTGGAACA ATGTCACAGT GTGGGGCTCT 
CGCTACGTGG TGGGGCAGAC GCCTGTCTAC GTTCCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC CGAGCCTGAC ACCATCATTG GGTACTCCGG AGAAGATTAT 
CCCAGGGCTG CGCCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GAGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GTGACCGTCC CCGGGGCGTG GTGGCCCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTGGTGGT CAATGGGAGA GGTGACGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGGT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA GTGGGACCAC CCAGAGCACC CCAACGGAAT CCTGATTGGA 
TACATCCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCTGTG CAGAGAGCAG ACCCAGTGTC GCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC ACAGGTGGGC TCTGGAGAAG CAGCCACAGA GGAGTCCCCA 
GCACCTCCAA ATGAAGCTAC TCCAACTGCA GCTCCTCCCA CGTTGCCCCC GACTACTGTG 
GGTACCACAG GCCTTGTGAG CAGTACTGAT GCTACTGCCC TTGCTGCCAC CAGTGAAGCC 
ACAACAGTTC CCATCATTCC AACCGTCGTA CCTACCACCG TCGCCACCAC CATTGCCACA 
ACTACTACAA CCACTGCCGC CACCACCACC ACCACCACTA CGGAGAGCCC TCCCACTACC 
ACTGCTGGGA CTAAGATTCA CGAAACCGCC CCCGACGAGC AGTCCATTTG GAACGTCACA 
GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCAG GCCTGGAACT 
GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC TGTTAAGGCC 
CAGGCCCAGC CTATACAGCT GACAGACCTC TTTCCCGGGA TGACGTACAC GTTGCGGGTG 
TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT 
TACACCAATA ACCAGGCAGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGTGCC 
ATTGCCCTTC TGGTGCTGAT CCTTCTCATC GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC 
AAGTACCCAG TGCGGGAAAA GAAGGATGTC CCCTTGGGTC CTGAAGACCC CAAAGAAGAA 
GATGGCTCAT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCT 
CTGGATGGCA CCATCAAGCA GCAGGAGAGC GATGACAGCC TGGTGGACTA TGGCGAAGGC 
GGCGAGGGCC AGTTCAATGA AGATGGCTCC TTTATTGGCC AGTACACTGT CAAAAAGGAC 
AAGGAGGAAA CGGAGGGCAA TGAGAGCTCA GAGGCCACAT CACCAGTCAA TGCCATCTAT 
TCCCTTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006529639.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529702.2 Mus musculus neurofascin (Nfasc), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu74371
Clone ID Related Accession (Same CDS sequence)	XM_006529639.3 , XM_006529639.4
Accession Version	XM_006529639.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529639.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CCCCCGACGA GCAGTCCATT 
TGGAACGTCA CAGTGCTCCC CAACAGTAAA TGGGCCAACA TCACCTGGAA GCACAATTTC 
AGGCCTGGAA CTGACTTTGT GGTTGAGTAC ATCGACAGCA ACCATACGAA AAAAACTGTC 
CCTGTTAAGG CCCAGGCCCA GCCTATACAG CTGACAGACC TCTTTCCCGG GATGACGTAC 
ACGTTGCGGG TGTATTCCCG GGACAACGAG GGCATCAGCA GTACCGTCAT CACCTTTATG 
ACCAGTACAG CTTACACCAA TAACCAGGCA GACATCGCCA CCCAGGGCTG GTTCATCGGG 
CTCATGTGTG CCATTGCCCT TCTGGTGCTG ATCCTTCTCA TCGTCTGCTT CATCAAGAGG 
AGTCGAGGTG GCAAGTACCC AGTGCGGGAA AAGAAGGATG TCCCCTTGGG TCCTGAAGAC 
CCCAAAGAAG AAGATGGCTC ATTTGACTAC AGTGATGAGG ACAACAAGCC CCTGCAGGGC 
AGCCAGACAT CTCTGGATGG CACCATCAAG CAGCAGGAGA GCGATGACAG CCTGGTGGAC 
TATGGCGAAG GCGGCGAGGG CCAGTTCAAT GAAGATGGCT CCTTTATTGG CCAGTACACT 
GTCAAAAAGG ACAAGGAGGA AACGGAGGGC AATGAGAGCT CAGAGGCCAC ATCACCAGTC 
AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529702.2
CDS	55..4218
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006529639.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529639.3

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CCCCCGACGA GCAGTCCATT 
TGGAACGTCA CAGTGCTCCC CAACAGTAAA TGGGCCAACA TCACCTGGAA GCACAATTTC 
AGGCCTGGAA CTGACTTTGT GGTTGAGTAC ATCGACAGCA ACCATACGAA AAAAACTGTC 
CCTGTTAAGG CCCAGGCCCA GCCTATACAG CTGACAGACC TCTTTCCCGG GATGACGTAC 
ACGTTGCGGG TGTATTCCCG GGACAACGAG GGCATCAGCA GTACCGTCAT CACCTTTATG 
ACCAGTACAG CTTACACCAA TAACCAGGCA GACATCGCCA CCCAGGGCTG GTTCATCGGG 
CTCATGTGTG CCATTGCCCT TCTGGTGCTG ATCCTTCTCA TCGTCTGCTT CATCAAGAGG 
AGTCGAGGTG GCAAGTACCC AGTGCGGGAA AAGAAGGATG TCCCCTTGGG TCCTGAAGAC 
CCCAAAGAAG AAGATGGCTC ATTTGACTAC AGTGATGAGG ACAACAAGCC CCTGCAGGGC 
AGCCAGACAT CTCTGGATGG CACCATCAAG CAGCAGGAGA GCGATGACAG CCTGGTGGAC 
TATGGCGAAG GCGGCGAGGG CCAGTTCAAT GAAGATGGCT CCTTTATTGG CCAGTACACT 
GTCAAAAAGG ACAAGGAGGA AACGGAGGGC AATGAGAGCT CAGAGGCCAC ATCACCAGTC 
AATGCCATCT ATTCCCTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248014.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246316.1 Mus musculus neurofascin (Nfasc), transcript variant X8, mRNA.

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CloneID	OMu74372
Clone ID Related Accession (Same CDS sequence)	XM_011248014.1 , XM_011248014.2
Accession Version	XM_011248014.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC GTTGCCCCCG 
ACTACTGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC 
AGTGAAGCCA CAACAGTTCC CATCATTCCA ACCGTCGTAC CTACCACCGT CGCCACCACC 
ATTGCCACAA CTACTACAAC CACTGCCGCC ACCACCACCA CCACCACTAC GGAGAGCCCT 
CCCACTACCA CTGCTGGGAC TAAGATTCAC GAAACCGCTT ACACCAATAA CCAGGCAGAC 
ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGTGCCA TTGCCCTTCT GGTGCTGATC 
CTTCTCATCG TCTGCTTCAT CAAGAGGAGT CGAGGTGGCA AGTACCCAGT GCGGGAAAAG 
AAGGATGTCC CCTTGGGTCC TGAAGACCCC AAAGAAGAAG ATGGCTCATT TGACTACAGT 
GATGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCTC TGGATGGCAC CATCAAGCAG 
CAGGAGAGCG ATGACAGCCT GGTGGACTAT GGCGAAGGCG GCGAGGGCCA GTTCAATGAA 
GATGGCTCCT TTATTGGCCA GTACACTGTC AAAAAGGACA AGGAGGAAAC GGAGGGCAAT 
GAGAGCTCAG AGGCCACATC ACCAGTCAAT GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246316.1
CDS	55..4185
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248014.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248014.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTATC CCAGGGCTGC GCCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG AGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG TGACCGTCCC CGGGGCGTGG TGGCCCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTGGTGGTC AATGGGAGAG GTGACGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGGTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAG TGGGACCACC CAGAGCACCC CAACGGAATC 
CTGATTGGAT ACATCCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCTGTGC AGAGAGCAGA CCCAGTGTCG 
CGTTACCGCT TCTCCCTCAG TGCCAGGACA CAGGTGGGCT CTGGAGAAGC AGCCACAGAG 
GAGTCCCCAG CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC GTTGCCCCCG 
ACTACTGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC 
AGTGAAGCCA CAACAGTTCC CATCATTCCA ACCGTCGTAC CTACCACCGT CGCCACCACC 
ATTGCCACAA CTACTACAAC CACTGCCGCC ACCACCACCA CCACCACTAC GGAGAGCCCT 
CCCACTACCA CTGCTGGGAC TAAGATTCAC GAAACCGCTT ACACCAATAA CCAGGCAGAC 
ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGTGCCA TTGCCCTTCT GGTGCTGATC 
CTTCTCATCG TCTGCTTCAT CAAGAGGAGT CGAGGTGGCA AGTACCCAGT GCGGGAAAAG 
AAGGATGTCC CCTTGGGTCC TGAAGACCCC AAAGAAGAAG ATGGCTCATT TGACTACAGT 
GATGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCTC TGGATGGCAC CATCAAGCAG 
CAGGAGAGCG ATGACAGCCT GGTGGACTAT GGCGAAGGCG GCGAGGGCCA GTTCAATGAA 
GATGGCTCCT TTATTGGCCA GTACACTGTC AAAAAGGACA AGGAGGAAAC GGAGGGCAAT 
GAGAGCTCAG AGGCCACATC ACCAGTCAAT GCCATCTATT CCCTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248015.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246317.1 Mus musculus neurofascin (Nfasc), transcript variant X9, mRNA.

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CloneID	OMu74373
Clone ID Related Accession (Same CDS sequence)	XM_011248015.2 , XM_011248015.1
Accession Version	XM_011248015.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTTAC CCAGTGCCCC CAGGCGGTTC AGAGTCCGAC AGCCCAACCT GGAGACCATC 
AACCTGGAGT GGGACCACCC AGAGCACCCC AACGGAATCC TGATTGGATA CATCCTCAGA 
TACGTGCCCT TTAATGGAAC CAAACTGGGA AAGCAGATGG TGGAAAACTT CTCTCCCAAT 
CAGACCAAGT TCTCTGTGCA GAGAGCAGAC CCAGTGTCGC GTTACCGCTT CTCCCTCAGT 
GCCAGGACAC AGGTGGGCTC TGGAGAAGCA GCCACAGAGG AGTCCCCAGC ACCTCCAAAT 
GAAGCTACTC CAACTGCAGC TCCTCCCACG TTGCCCCCGA CTACTGTGGG TACCACAGGC 
CTTGTGAGCA GTACTGATGC TACTGCCCTT GCTGCCACCA GTGAAGCCAC AACAGTTCCC 
ATCATTCCAA CCGTCGTACC TACCACCGTC GCCACCACCA TTGCCACAAC TACTACAACC 
ACTGCCGCCA CCACCACCAC CACCACTACG GAGAGCCCTC CCACTACCAC TGCTGGGACT 
AAGATTCACG AAACCGCCCC CGACGAGCAG TCCATTTGGA ACGTCACAGT GCTCCCCAAC 
AGTAAATGGG CCAACATCAC CTGGAAGCAC AATTTCAGGC CTGGAACTGA CTTTGTGGTT 
GAGTACATCG ACAGCAACCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCT 
ATACAGCTGA CAGACCTCTT TCCCGGGATG ACGTACACGT TGCGGGTGTA TTCCCGGGAC 
AACGAGGGCA TCAGCAGTAC CGTCATCACC TTTATGACCA GTACAGCTTA CACCAATAAC 
CAGGCAGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG 
GTGCTGATCC TTCTCATCGT CTGCTTCATC AAGAGGAGTC GAGGTGGCAA GTACCCAGTG 
CGGGAAAAGA AGGATGTCCC CTTGGGTCCT GAAGACCCCA AAGAAGAAGA TGGCTCATTT 
GACTACAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGATGGCACC 
ATCAAGCAGC AGGAGAGCGA TGACAGCCTG GTGGACTATG GCGAAGGCGG CGAGGGCCAG 
TTCAATGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA AAAAGGACAA GGAGGAAACG 
GAGGGCAATG AGAGCTCAGA GGCCACATCA CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246317.1
CDS	54..4133
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248015.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248015.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CAACAACCCC 
TATAATGACT CGTCCTTAAG AAACCACCCT GACATATATA GTGCCCGAGG AGTTGCAGAA 
AGAACGCCCA GCTTCATGTA TCCCCAGGGC ACATCGAGCA GTCAGATGGT TCTCCGTGGC 
ATGGACCTGC TGCTGGAATG CATTGCCTCT GGCGTCCCAA CACCAGACAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTAACAAG GCCAAGTTCG AGAACTTTAA TAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCATA CTGGCTGGAT 
GAGCCCAAGA ACCTGATCCT GGCTCCTGGG GAAGATGGGA GGCTGGTATG CCGAGCCAAT 
GGGAACCCGA AGCCGACCGT GCAGTGGATG GTGAATGGAG AGCCTTTACA ATCGGCACCA 
CCCAATCCCA ACCGTGAGGT AGCTGGAGAC ACTATCATCT TCCGGGATAC TCAGATCAGC 
AGCAGGGCAG TGTACCAATG TAATACATCC AATGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TGTTAGATGT ACCCCCTCGG ATGCTGTCTG CCCGCAACCA GCTCATCAGG 
GTGATCCTTT ATAACCGGAC ACGGCTGGAC TGTCCGTTCT TTGGGTCTCC CATCCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GCGGTAACTA CCACGTCTAC 
GAAAACGGCA GTCTAGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG CAAAGCCGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC CGAGGACCAG GTGGCCAAGA GGGGCACCAC GGTGCAGCTG 
GAGTGCCGCG TGAAACATGA CCCCTCCTTG AAGCTCACAG TCTCCTGGCT GAAGGACGAT 
GAGCCACTCT ACATTGGAAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC GATCTTCGGA 
GTGGCAGAGC GGGACCAGGG CAGTTACACG TGTATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCAACTAA CCGTTTGGCT 
GCCCTACCCA AAGGGCGACC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCTGAG 
AGGAGTGTGA GGCTGACCTG GATCCCAGGG GATGACAACA ACAGCCCTAT CACAGACTAC 
GTCGTTCAGT TTGAAGAGGA CCAGTTCCAA CCAGGGGTGT GGCATGACCA CTCCAGGTTC 
CCAGGCAGCG TCAACTCAGC CGTCCTCCAT CTGTCCCCAT ATGTCAACTA CCAATTCAGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACCAGTGGGG CACCCCCTGA ATCTAATCCC AGTGATGTGA AGGGCGAAGG GACAAGAAAG 
AACAATATGG AGATCACGTG GACGCCTATG AATGCTACCT CTGCCTTTGG CCCCAACCTA 
CGCTACATTG TCAAGTGGCG ACGGAGAGAG ACCCGAGAGA CTTGGAACAA TGTCACAGTG 
TGGGGCTCTC GCTACGTGGT GGGGCAGACG CCTGTCTACG TTCCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCC GAGCCTGACA CCATCATTGG GTACTCCGGA 
GAAGATTTAC CCAGTGCCCC CAGGCGGTTC AGAGTCCGAC AGCCCAACCT GGAGACCATC 
AACCTGGAGT GGGACCACCC AGAGCACCCC AACGGAATCC TGATTGGATA CATCCTCAGA 
TACGTGCCCT TTAATGGAAC CAAACTGGGA AAGCAGATGG TGGAAAACTT CTCTCCCAAT 
CAGACCAAGT TCTCTGTGCA GAGAGCAGAC CCAGTGTCGC GTTACCGCTT CTCCCTCAGT 
GCCAGGACAC AGGTGGGCTC TGGAGAAGCA GCCACAGAGG AGTCCCCAGC ACCTCCAAAT 
GAAGCTACTC CAACTGCAGC TCCTCCCACG TTGCCCCCGA CTACTGTGGG TACCACAGGC 
CTTGTGAGCA GTACTGATGC TACTGCCCTT GCTGCCACCA GTGAAGCCAC AACAGTTCCC 
ATCATTCCAA CCGTCGTACC TACCACCGTC GCCACCACCA TTGCCACAAC TACTACAACC 
ACTGCCGCCA CCACCACCAC CACCACTACG GAGAGCCCTC CCACTACCAC TGCTGGGACT 
AAGATTCACG AAACCGCCCC CGACGAGCAG TCCATTTGGA ACGTCACAGT GCTCCCCAAC 
AGTAAATGGG CCAACATCAC CTGGAAGCAC AATTTCAGGC CTGGAACTGA CTTTGTGGTT 
GAGTACATCG ACAGCAACCA TACGAAAAAA ACTGTCCCTG TTAAGGCCCA GGCCCAGCCT 
ATACAGCTGA CAGACCTCTT TCCCGGGATG ACGTACACGT TGCGGGTGTA TTCCCGGGAC 
AACGAGGGCA TCAGCAGTAC CGTCATCACC TTTATGACCA GTACAGCTTA CACCAATAAC 
CAGGCAGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG 
GTGCTGATCC TTCTCATCGT CTGCTTCATC AAGAGGAGTC GAGGTGGCAA GTACCCAGTG 
CGGGAAAAGA AGGATGTCCC CTTGGGTCCT GAAGACCCCA AAGAAGAAGA TGGCTCATTT 
GACTACAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGATGGCACC 
ATCAAGCAGC AGGAGAGCGA TGACAGCCTG GTGGACTATG GCGAAGGCGG CGAGGGCCAG 
TTCAATGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA AAAAGGACAA GGAGGAAACG 
GAGGGCAATG AGAGCTCAGA GGCCACATCA CCAGTCAATG CCATCTATTC CCTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248016.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246318.1 Mus musculus neurofascin (Nfasc), transcript variant X12, mRNA.

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CloneID	OMu74374
Clone ID Related Accession (Same CDS sequence)	XM_011248016.1
Accession Version	XM_011248016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CACCCGAGGA 
GTTGCAGAAA GAACGCCCAG CTTCATGTAT CCCCAGGGCA CATCGAGCAG TCAGATGGTT 
CTCCGTGGCA TGGACCTGCT GCTGGAATGC ATTGCCTCTG GCGTCCCAAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTAACAAGG CCAAGTTCGA GAACTTTAAT 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCTCCATAC 
TGGCTGGATG AGCCCAAGAA CCTGATCCTG GCTCCTGGGG AAGATGGGAG GCTGGTATGC 
CGAGCCAATG GGAACCCGAA GCCGACCGTG CAGTGGATGG TGAATGGAGA GCCTTTACAA 
TCGGCACCAC CCAATCCCAA CCGTGAGGTA GCTGGAGACA CTATCATCTT CCGGGATACT 
CAGATCAGCA GCAGGGCAGT GTACCAATGT AATACATCCA ATGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT GTTAGATGTA CCCCCTCGGA TGCTGTCTGC CCGCAACCAG 
CTCATCAGGG TGATCCTTTA TAACCGGACA CGGCTGGACT GTCCGTTCTT TGGGTCTCCC 
ATCCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG CGGTAACTAC 
CACGTCTACG AAAACGGCAG TCTAGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGC AAAGCCGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCC GAGGACCAGG TGGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAACATGAC CCCTCCTTGA AGCTCACAGT CTCCTGGCTG 
AAGGACGATG AGCCACTCTA CATTGGAAAC AGGATGAAGA AGGAAGATGA CTCCCTGACG 
ATCTTCGGAG TGGCAGAGCG GGACCAGGGC AGTTACACGT GTATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTACCCAA AGGGCGACCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCTGAGA GGAGTGTGAG GCTGACCTGG ATCCCAGGGG ATGACAACAA CAGCCCTATC 
ACAGACTACG TCGTTCAGTT TGAAGAGGAC CAGTTCCAAC CAGGGGTGTG GCATGACCAC 
TCCAGGTTCC CAGGCAGCGT CAACTCAGCC GTCCTCCATC TGTCCCCATA TGTCAACTAC 
CAATTCAGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CCAGTGGGGC ACCCCCTGAA TCTAATCCCA GTGATGTGAA GGGCGAAGGG 
ACAAGAAAGA ACAATATGGA GATCACGTGG ACGCCTATGA ATGCTACCTC TGCCTTTGGC 
CCCAACCTAC GCTACATTGT CAAGTGGCGA CGGAGAGAGA CCCGAGAGAC TTGGAACAAT 
GTCACAGTGT GGGGCTCTCG CTACGTGGTG GGGCAGACGC CTGTCTACGT TCCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCCG AGCCTGACAC CATCATTGGG 
TACTCCGGAG AAGATTTACC CAGTGCCCCC AGGCGGTTCA GAGTCCGACA GCCCAACCTG 
GAGACCATCA ACCTGGAGTG GGACCACCCA GAGCACCCCA ACGGAATCCT GATTGGATAC 
ATCCTCAGAT ACGTGCCCTT TAATGGAACC AAACTGGGAA AGCAGATGGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CTCTGTGCAG AGAGCAGACC CAGTGTCGCG TTACCGCTTC 
TCCCTCAGTG CCAGGACACA GGTGGGCTCT GGAGAAGCAG CCACAGAGGA GTCCCCAGCA 
CCTCCAAATG AAGCTACTCC AACTGCAGCT CCTCCCACGT TGCCCCCGAC TACTGTGGGT 
ACCACAGGCC TTGTGAGCAG TACTGATGCT ACTGCCCTTG CTGCCACCAG TGAAGCCACA 
ACAGTTCCCA TCATTCCAAC CGTCGTACCT ACCACCGTCG CCACCACCAT TGCCACAACT 
ACTACAACCA CTGCCGCCAC CACCACCACC ACCACTACGG AGAGCCCTCC CACTACCACT 
GCTGGGACTA AGATTCACGA AACCGCCCCC GACGAGCAGT CCATTTGGAA CGTCACAGTG 
CTCCCCAACA GTAAATGGGC CAACATCACC TGGAAGCACA ATTTCAGGCC TGGAACTGAC 
TTTGTGGTTG AGTACATCGA CAGCAACCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG 
GCCCAGCCTA TACAGCTGAC AGACCTCTTT CCCGGGATGA CGTACACGTT GCGGGTGTAT 
TCCCGGGACA ACGAGGGCAT CAGCAGTACC GTCATCACCT TTATGACCAG TACAGCTTAC 
ACCAATAACC AGGCAGACAT CGCCACCCAG GGCTGGTTCA TCGGGCTCAT GTGTGCCATT 
GCCCTTCTGG TGCTGATCCT TCTCATCGTC TGCTTCATCA AGAGGAGTCG AGGTGGCAAG 
TACCCAGTGC GGGAAAAGAA GGATGTCCCC TTGGGTCCTG AAGACCCCAA AGAAGAAGAT 
GGCTCATTTG ACTACAGTGA TGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACATCTCTG 
GATGGCACCA TCAAGCAGCA GGAGAGCGAT GACAGCCTGG TGGACTATGG CGAAGGCGGC 
GAGGGCCAGT TCAATGAAGA TGGCTCCTTT ATTGGCCAGT ACACTGTCAA AAAGGACAAG 
GAGGAAACGG AGGGCAATGA GAGCTCAGAG GCCACATCAC CAGTCAATGC CATCTATTCC 
CTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246318.1
CDS	53..4081
Translation	MRRLAARGAAGDAREVAAPGADIWDCARGWSPSSSRLTDLCALWQPGVFLPSADGSRASAGVGKSRPNEAEKLSTVWKAHIERHKASGARGLGSGEQGLVLRMARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248016.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248016.1

ORF Insert Sequence:

ATGCGGCGGC TGGCAGCGAG AGGTGCTGCA GGGGACGCGA GGGAAGTGGC GGCGCCGGGA 
GCGGACATCT GGGACTGCGC CCGGGGCTGG AGCCCGAGCT CCTCGAGGTT GACTGACTTA 
TGTGCGCTTT GGCAGCCTGG AGTTTTCTTG CCCTCTGCCG ACGGGAGCAG AGCCTCTGCT 
GGTGTTGGGA AAAGCAGGCC GAATGAGGCA GAGAAGCTGA GCACTGTCTG GAAAGCCCAC 
ATAGAGCGGC ACAAGGCATC GGGAGCCCGT GGGCTGGGCA GCGGGGAGCA GGGCCTGGTG 
CTGAGGATGG CCAGGCAGCA GGCGCCACCC TGGGTCCACA TAGCCCTCAT CCTCTTCCTC 
CTCAGCCTCG GAGGGGCCAT CGAGATTCCG ATGGACCCAA GCATTCAGAA TGAGCTGACC 
CAACCCCCAA CTATCACCAA GCAGTCCGTG AAGGACCACA TCGTGGACCC TCGAGATAAC 
ATCCTGATTG AATGTGAAGC TAAAGGCAAC CCCGCCCCCA GTTTTCACTG GACTCGCAAC 
AGCAGATTCT TCAACATTGC CAAGGACCCA CGGGTGTCCA TGAGGAGGAG ATCTGGGACC 
TTGGTGATCG ACTTCCGCAG TGGTGGGCGG CCTGAGGAAT ACGAAGGGGA GTACCAGTGC 
TTTGCCCGGA ACAAATTTGG CACTGCACTT AGCAACCGCA TCCGCCTGCA GGTGTCCAAA 
TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCCGTCGTGG TTCAAGAGGG TGCCCCCTTG 
ACACTACAGT GCAACCCCCC ACCCGGCCTC CCGTCCCCCG TCATCTTCTG GATGAGCAGC 
TCCATGGAGC CCATCACCCA GGACAAGCGT GTCTCCCAGG GTCACAACGG GGACCTGTAC 
TTCTCCAACG TCATGCTGCA GGACATGCAG ACCGACTACA GCTGCAACGC GCGCTTTCAC 
TTCACCCACA CCATTCAGCA GAAGAACCCC TTCACCCTCA AGGTCCTCAC CACCCGAGGA 
GTTGCAGAAA GAACGCCCAG CTTCATGTAT CCCCAGGGCA CATCGAGCAG TCAGATGGTT 
CTCCGTGGCA TGGACCTGCT GCTGGAATGC ATTGCCTCTG GCGTCCCAAC ACCAGACATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTAACAAGG CCAAGTTCGA GAACTTTAAT 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCTCCATAC 
TGGCTGGATG AGCCCAAGAA CCTGATCCTG GCTCCTGGGG AAGATGGGAG GCTGGTATGC 
CGAGCCAATG GGAACCCGAA GCCGACCGTG CAGTGGATGG TGAATGGAGA GCCTTTACAA 
TCGGCACCAC CCAATCCCAA CCGTGAGGTA GCTGGAGACA CTATCATCTT CCGGGATACT 
CAGATCAGCA GCAGGGCAGT GTACCAATGT AATACATCCA ATGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT GTTAGATGTA CCCCCTCGGA TGCTGTCTGC CCGCAACCAG 
CTCATCAGGG TGATCCTTTA TAACCGGACA CGGCTGGACT GTCCGTTCTT TGGGTCTCCC 
ATCCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG CGGTAACTAC 
CACGTCTACG AAAACGGCAG TCTAGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGC AAAGCCGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCC GAGGACCAGG TGGCCAAGAG GGGCACCACG 
GTGCAGCTGG AGTGCCGCGT GAAACATGAC CCCTCCTTGA AGCTCACAGT CTCCTGGCTG 
AAGGACGATG AGCCACTCTA CATTGGAAAC AGGATGAAGA AGGAAGATGA CTCCCTGACG 
ATCTTCGGAG TGGCAGAGCG GGACCAGGGC AGTTACACGT GTATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCAACTAAC 
CGTTTGGCTG CCCTACCCAA AGGGCGACCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCTGAGA GGAGTGTGAG GCTGACCTGG ATCCCAGGGG ATGACAACAA CAGCCCTATC 
ACAGACTACG TCGTTCAGTT TGAAGAGGAC CAGTTCCAAC CAGGGGTGTG GCATGACCAC 
TCCAGGTTCC CAGGCAGCGT CAACTCAGCC GTCCTCCATC TGTCCCCATA TGTCAACTAC 
CAATTCAGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CCAGTGGGGC ACCCCCTGAA TCTAATCCCA GTGATGTGAA GGGCGAAGGG 
ACAAGAAAGA ACAATATGGA GATCACGTGG ACGCCTATGA ATGCTACCTC TGCCTTTGGC 
CCCAACCTAC GCTACATTGT CAAGTGGCGA CGGAGAGAGA CCCGAGAGAC TTGGAACAAT 
GTCACAGTGT GGGGCTCTCG CTACGTGGTG GGGCAGACGC CTGTCTACGT TCCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCCG AGCCTGACAC CATCATTGGG 
TACTCCGGAG AAGATTTACC CAGTGCCCCC AGGCGGTTCA GAGTCCGACA GCCCAACCTG 
GAGACCATCA ACCTGGAGTG GGACCACCCA GAGCACCCCA ACGGAATCCT GATTGGATAC 
ATCCTCAGAT ACGTGCCCTT TAATGGAACC AAACTGGGAA AGCAGATGGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CTCTGTGCAG AGAGCAGACC CAGTGTCGCG TTACCGCTTC 
TCCCTCAGTG CCAGGACACA GGTGGGCTCT GGAGAAGCAG CCACAGAGGA GTCCCCAGCA 
CCTCCAAATG AAGCTACTCC AACTGCAGCT CCTCCCACGT TGCCCCCGAC TACTGTGGGT 
ACCACAGGCC TTGTGAGCAG TACTGATGCT ACTGCCCTTG CTGCCACCAG TGAAGCCACA 
ACAGTTCCCA TCATTCCAAC CGTCGTACCT ACCACCGTCG CCACCACCAT TGCCACAACT 
ACTACAACCA CTGCCGCCAC CACCACCACC ACCACTACGG AGAGCCCTCC CACTACCACT 
GCTGGGACTA AGATTCACGA AACCGCCCCC GACGAGCAGT CCATTTGGAA CGTCACAGTG 
CTCCCCAACA GTAAATGGGC CAACATCACC TGGAAGCACA ATTTCAGGCC TGGAACTGAC 
TTTGTGGTTG AGTACATCGA CAGCAACCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAG 
GCCCAGCCTA TACAGCTGAC AGACCTCTTT CCCGGGATGA CGTACACGTT GCGGGTGTAT 
TCCCGGGACA ACGAGGGCAT CAGCAGTACC GTCATCACCT TTATGACCAG TACAGCTTAC 
ACCAATAACC AGGCAGACAT CGCCACCCAG GGCTGGTTCA TCGGGCTCAT GTGTGCCATT 
GCCCTTCTGG TGCTGATCCT TCTCATCGTC TGCTTCATCA AGAGGAGTCG AGGTGGCAAG 
TACCCAGTGC GGGAAAAGAA GGATGTCCCC TTGGGTCCTG AAGACCCCAA AGAAGAAGAT 
GGCTCATTTG ACTACAGTGA TGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACATCTCTG 
GATGGCACCA TCAAGCAGCA GGAGAGCGAT GACAGCCTGG TGGACTATGG CGAAGGCGGC 
GAGGGCCAGT TCAATGAAGA TGGCTCCTTT ATTGGCCAGT ACACTGTCAA AAAGGACAAG 
GAGGAAACGG AGGGCAATGA GAGCTCAGAG GCCACATCAC CAGTCAATGC CATCTATTCC 
CTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248024.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246326.1 Mus musculus neurofascin (Nfasc), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OMu74382
Clone ID Related Accession (Same CDS sequence)	XM_011248024.2 , XM_011248023.1 , XM_011248019.1 , XM_011248024.3
Accession Version	XM_011248024.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248024.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACTATCA CCAAGCAGTC CGTGAAGGAC CACATCGTGG ACCCTCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG CAACCCCGCC CCCAGTTTTC ACTGGACTCG CAACAGCAGA 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGATCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAATACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TTGGCACTGC ACTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTGACACTA 
CAGTGCAACC CCCCACCCGG CCTCCCGTCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ACGCGCGCTT TCACTTCACC 
CACACCATTC AGCAGAAGAA CCCCTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC AGAAAGAACG 
CCCAGCTTCA TGTATCCCCA GGGCACATCG AGCAGTCAGA TGGTTCTCCG TGGCATGGAC 
CTGCTGCTGG AATGCATTGC CTCTGGCGTC CCAACACCAG ACATTGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTAA CAAGGCCAAG TTCGAGAACT TTAATAAGGC TCTGCGCATC 
ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CATACTGGCT GGATGAGCCC 
AAGAACCTGA TCCTGGCTCC TGGGGAAGAT GGGAGGCTGG TATGCCGAGC CAATGGGAAC 
CCGAAGCCGA CCGTGCAGTG GATGGTGAAT GGAGAGCCTT TACAATCGGC ACCACCCAAT 
CCCAACCGTG AGGTAGCTGG AGACACTATC ATCTTCCGGG ATACTCAGAT CAGCAGCAGG 
GCAGTGTACC AATGTAATAC ATCCAATGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC 
AGCGTGTTAG ATGTACCCCC TCGGATGCTG TCTGCCCGCA ACCAGCTCAT CAGGGTGATC 
CTTTATAACC GGACACGGCT GGACTGTCCG TTCTTTGGGT CTCCCATCCC AACACTCCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGCGGTA ACTACCACGT CTACGAAAAC 
GGCAGTCTAG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC 
ACCAACATCC TGGGCAAAGC CGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACAGGA TGCCCGAGGA CCAGGTGGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC 
CGCGTGAAAC ATGACCCCTC CTTGAAGCTC ACAGTCTCCT GGCTGAAGGA CGATGAGCCA 
CTCTACATTG GAAACAGGAT GAAGAAGGAA GATGACTCCC TGACGATCTT CGGAGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTATG GCCAGCACCG AGCTGGACCA GGACCTGGCA 
AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTA 
CCCAAAGGGC GACCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT 
GTGAGGCTGA CCTGGATCCC AGGGGATGAC AACAACAGCC CTATCACAGA CTACGTCGTT 
CAGTTTGAAG AGGACCAGTT CCAACCAGGG GTGTGGCATG ACCACTCCAG GTTCCCAGGC 
AGCGTCAACT CAGCCGTCCT CCATCTGTCC CCATATGTCA ACTACCAATT CAGAGTCATC 
GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACCAGT 
GGGGCACCCC CTGAATCTAA TCCCAGTGAT GTGAAGGGCG AAGGGACAAG AAAGAACAAT 
ATGGAGATCA CGTGGACGCC TATGAATGCT ACCTCTGCCT TTGGCCCCAA CCTACGCTAC 
ATTGTCAAGT GGCGACGGAG AGAGACCCGA GAGACTTGGA ACAATGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACGCCTGTC TACGTTCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TTGGGAAAGG CCCCGAGCCT GACACCATCA TTGGGTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGAGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT 
TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG 
GCCAGCTTCC CTGGTGACCG TCCCCGGGGC GTGGTGGCCC GCCTGTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTGGT GGTCAATGGG AGAGGTGACG GGCCTCGAAG TGAAACCAAG 
GAATTCACCA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GGTTCAGAGT CCGACAGCCC 
AACCTGGAGA CCATCAACCT GGAGTGGGAC CACCCAGAGC ACCCCAACGG AATCCTGATT 
GGATACATCC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCTCT GTGCAGAGAG CAGACCCAGT GTCGCGTTAC 
CGCTTCTCCC TCAGTGCCAG GACACAGGTG GGCTCTGGAG AAGCAGCCAC AGAGGAGTCC 
CCAGCACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACGTTGCC CCCGACTACT 
GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA 
GCCACAACAG TTCCCATCAT TCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC 
ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CTACGGAGAG CCCTCCCACT 
ACCACTGCTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC 
ACAGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA 
ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG 
GCCCAGGCCC AGCCTATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG 
GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA 
GCTTACACCA ATAACCAGGC AGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT 
GCCATTGCCC TTCTGGTGCT GATCCTTCTC ATCGTCTGCT TCATCAAGAG GAGTCGAGGT 
GGCAAGTACC CAGTGCGGGA AAAGAAGGAT GTCCCCTTGG GTCCTGAAGA CCCCAAAGAA 
GAAGATGGCT CATTTGACTA CAGTGATGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA 
TCTCTGGATG GCACCATCAA GCAGCAGGAG AGCGATGACA GCCTGGTGGA CTATGGCGAA 
GGCGGCGAGG GCCAGTTCAA TGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAAAAAG 
GACAAGGAGG AAACGGAGGG CAATGAGAGC TCAGAGGCCA CATCACCAGT CAATGCCATC 
TATTCCCTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246326.1
CDS	115..4209
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248024.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248024.2

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACTATCA CCAAGCAGTC CGTGAAGGAC CACATCGTGG ACCCTCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG CAACCCCGCC CCCAGTTTTC ACTGGACTCG CAACAGCAGA 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGATCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAATACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TTGGCACTGC ACTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTGACACTA 
CAGTGCAACC CCCCACCCGG CCTCCCGTCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ACGCGCGCTT TCACTTCACC 
CACACCATTC AGCAGAAGAA CCCCTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC AGAAAGAACG 
CCCAGCTTCA TGTATCCCCA GGGCACATCG AGCAGTCAGA TGGTTCTCCG TGGCATGGAC 
CTGCTGCTGG AATGCATTGC CTCTGGCGTC CCAACACCAG ACATTGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTAA CAAGGCCAAG TTCGAGAACT TTAATAAGGC TCTGCGCATC 
ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CATACTGGCT GGATGAGCCC 
AAGAACCTGA TCCTGGCTCC TGGGGAAGAT GGGAGGCTGG TATGCCGAGC CAATGGGAAC 
CCGAAGCCGA CCGTGCAGTG GATGGTGAAT GGAGAGCCTT TACAATCGGC ACCACCCAAT 
CCCAACCGTG AGGTAGCTGG AGACACTATC ATCTTCCGGG ATACTCAGAT CAGCAGCAGG 
GCAGTGTACC AATGTAATAC ATCCAATGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC 
AGCGTGTTAG ATGTACCCCC TCGGATGCTG TCTGCCCGCA ACCAGCTCAT CAGGGTGATC 
CTTTATAACC GGACACGGCT GGACTGTCCG TTCTTTGGGT CTCCCATCCC AACACTCCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGCGGTA ACTACCACGT CTACGAAAAC 
GGCAGTCTAG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC 
ACCAACATCC TGGGCAAAGC CGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACAGGA TGCCCGAGGA CCAGGTGGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC 
CGCGTGAAAC ATGACCCCTC CTTGAAGCTC ACAGTCTCCT GGCTGAAGGA CGATGAGCCA 
CTCTACATTG GAAACAGGAT GAAGAAGGAA GATGACTCCC TGACGATCTT CGGAGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTATG GCCAGCACCG AGCTGGACCA GGACCTGGCA 
AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTA 
CCCAAAGGGC GACCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT 
GTGAGGCTGA CCTGGATCCC AGGGGATGAC AACAACAGCC CTATCACAGA CTACGTCGTT 
CAGTTTGAAG AGGACCAGTT CCAACCAGGG GTGTGGCATG ACCACTCCAG GTTCCCAGGC 
AGCGTCAACT CAGCCGTCCT CCATCTGTCC CCATATGTCA ACTACCAATT CAGAGTCATC 
GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACCAGT 
GGGGCACCCC CTGAATCTAA TCCCAGTGAT GTGAAGGGCG AAGGGACAAG AAAGAACAAT 
ATGGAGATCA CGTGGACGCC TATGAATGCT ACCTCTGCCT TTGGCCCCAA CCTACGCTAC 
ATTGTCAAGT GGCGACGGAG AGAGACCCGA GAGACTTGGA ACAATGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACGCCTGTC TACGTTCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TTGGGAAAGG CCCCGAGCCT GACACCATCA TTGGGTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGAGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT 
TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG 
GCCAGCTTCC CTGGTGACCG TCCCCGGGGC GTGGTGGCCC GCCTGTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTGGT GGTCAATGGG AGAGGTGACG GGCCTCGAAG TGAAACCAAG 
GAATTCACCA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GGTTCAGAGT CCGACAGCCC 
AACCTGGAGA CCATCAACCT GGAGTGGGAC CACCCAGAGC ACCCCAACGG AATCCTGATT 
GGATACATCC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCTCT GTGCAGAGAG CAGACCCAGT GTCGCGTTAC 
CGCTTCTCCC TCAGTGCCAG GACACAGGTG GGCTCTGGAG AAGCAGCCAC AGAGGAGTCC 
CCAGCACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACGTTGCC CCCGACTACT 
GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA 
GCCACAACAG TTCCCATCAT TCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC 
ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CTACGGAGAG CCCTCCCACT 
ACCACTGCTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC 
ACAGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA 
ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG 
GCCCAGGCCC AGCCTATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG 
GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA 
GCTTACACCA ATAACCAGGC AGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT 
GCCATTGCCC TTCTGGTGCT GATCCTTCTC ATCGTCTGCT TCATCAAGAG GAGTCGAGGT 
GGCAAGTACC CAGTGCGGGA AAAGAAGGAT GTCCCCTTGG GTCCTGAAGA CCCCAAAGAA 
GAAGATGGCT CATTTGACTA CAGTGATGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA 
TCTCTGGATG GCACCATCAA GCAGCAGGAG AGCGATGACA GCCTGGTGGA CTATGGCGAA 
GGCGGCGAGG GCCAGTTCAA TGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAAAAAG 
GACAAGGAGG AAACGGAGGG CAATGAGAGC TCAGAGGCCA CATCACCAGT CAATGCCATC 
TATTCCCTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248023.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246325.1 Mus musculus neurofascin (Nfasc), transcript variant X32, mRNA.

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CloneID	OMu74382
Clone ID Related Accession (Same CDS sequence)	XM_011248024.2 , XM_011248023.1 , XM_011248019.1 , XM_011248024.3
Accession Version	XM_011248023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACTATCA CCAAGCAGTC CGTGAAGGAC CACATCGTGG ACCCTCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG CAACCCCGCC CCCAGTTTTC ACTGGACTCG CAACAGCAGA 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGATCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAATACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TTGGCACTGC ACTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTGACACTA 
CAGTGCAACC CCCCACCCGG CCTCCCGTCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ACGCGCGCTT TCACTTCACC 
CACACCATTC AGCAGAAGAA CCCCTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC AGAAAGAACG 
CCCAGCTTCA TGTATCCCCA GGGCACATCG AGCAGTCAGA TGGTTCTCCG TGGCATGGAC 
CTGCTGCTGG AATGCATTGC CTCTGGCGTC CCAACACCAG ACATTGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTAA CAAGGCCAAG TTCGAGAACT TTAATAAGGC TCTGCGCATC 
ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CATACTGGCT GGATGAGCCC 
AAGAACCTGA TCCTGGCTCC TGGGGAAGAT GGGAGGCTGG TATGCCGAGC CAATGGGAAC 
CCGAAGCCGA CCGTGCAGTG GATGGTGAAT GGAGAGCCTT TACAATCGGC ACCACCCAAT 
CCCAACCGTG AGGTAGCTGG AGACACTATC ATCTTCCGGG ATACTCAGAT CAGCAGCAGG 
GCAGTGTACC AATGTAATAC ATCCAATGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC 
AGCGTGTTAG ATGTACCCCC TCGGATGCTG TCTGCCCGCA ACCAGCTCAT CAGGGTGATC 
CTTTATAACC GGACACGGCT GGACTGTCCG TTCTTTGGGT CTCCCATCCC AACACTCCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGCGGTA ACTACCACGT CTACGAAAAC 
GGCAGTCTAG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC 
ACCAACATCC TGGGCAAAGC CGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACAGGA TGCCCGAGGA CCAGGTGGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC 
CGCGTGAAAC ATGACCCCTC CTTGAAGCTC ACAGTCTCCT GGCTGAAGGA CGATGAGCCA 
CTCTACATTG GAAACAGGAT GAAGAAGGAA GATGACTCCC TGACGATCTT CGGAGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTATG GCCAGCACCG AGCTGGACCA GGACCTGGCA 
AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTA 
CCCAAAGGGC GACCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT 
GTGAGGCTGA CCTGGATCCC AGGGGATGAC AACAACAGCC CTATCACAGA CTACGTCGTT 
CAGTTTGAAG AGGACCAGTT CCAACCAGGG GTGTGGCATG ACCACTCCAG GTTCCCAGGC 
AGCGTCAACT CAGCCGTCCT CCATCTGTCC CCATATGTCA ACTACCAATT CAGAGTCATC 
GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACCAGT 
GGGGCACCCC CTGAATCTAA TCCCAGTGAT GTGAAGGGCG AAGGGACAAG AAAGAACAAT 
ATGGAGATCA CGTGGACGCC TATGAATGCT ACCTCTGCCT TTGGCCCCAA CCTACGCTAC 
ATTGTCAAGT GGCGACGGAG AGAGACCCGA GAGACTTGGA ACAATGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACGCCTGTC TACGTTCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TTGGGAAAGG CCCCGAGCCT GACACCATCA TTGGGTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGAGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT 
TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG 
GCCAGCTTCC CTGGTGACCG TCCCCGGGGC GTGGTGGCCC GCCTGTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTGGT GGTCAATGGG AGAGGTGACG GGCCTCGAAG TGAAACCAAG 
GAATTCACCA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GGTTCAGAGT CCGACAGCCC 
AACCTGGAGA CCATCAACCT GGAGTGGGAC CACCCAGAGC ACCCCAACGG AATCCTGATT 
GGATACATCC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCTCT GTGCAGAGAG CAGACCCAGT GTCGCGTTAC 
CGCTTCTCCC TCAGTGCCAG GACACAGGTG GGCTCTGGAG AAGCAGCCAC AGAGGAGTCC 
CCAGCACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACGTTGCC CCCGACTACT 
GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA 
GCCACAACAG TTCCCATCAT TCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC 
ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CTACGGAGAG CCCTCCCACT 
ACCACTGCTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC 
ACAGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA 
ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG 
GCCCAGGCCC AGCCTATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG 
GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA 
GCTTACACCA ATAACCAGGC AGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT 
GCCATTGCCC TTCTGGTGCT GATCCTTCTC ATCGTCTGCT TCATCAAGAG GAGTCGAGGT 
GGCAAGTACC CAGTGCGGGA AAAGAAGGAT GTCCCCTTGG GTCCTGAAGA CCCCAAAGAA 
GAAGATGGCT CATTTGACTA CAGTGATGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA 
TCTCTGGATG GCACCATCAA GCAGCAGGAG AGCGATGACA GCCTGGTGGA CTATGGCGAA 
GGCGGCGAGG GCCAGTTCAA TGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAAAAAG 
GACAAGGAGG AAACGGAGGG CAATGAGAGC TCAGAGGCCA CATCACCAGT CAATGCCATC 
TATTCCCTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246325.1
CDS	280..4374
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248023.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248023.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACTATCA CCAAGCAGTC CGTGAAGGAC CACATCGTGG ACCCTCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG CAACCCCGCC CCCAGTTTTC ACTGGACTCG CAACAGCAGA 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGATCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAATACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TTGGCACTGC ACTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTGACACTA 
CAGTGCAACC CCCCACCCGG CCTCCCGTCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ACGCGCGCTT TCACTTCACC 
CACACCATTC AGCAGAAGAA CCCCTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC AGAAAGAACG 
CCCAGCTTCA TGTATCCCCA GGGCACATCG AGCAGTCAGA TGGTTCTCCG TGGCATGGAC 
CTGCTGCTGG AATGCATTGC CTCTGGCGTC CCAACACCAG ACATTGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTAA CAAGGCCAAG TTCGAGAACT TTAATAAGGC TCTGCGCATC 
ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CATACTGGCT GGATGAGCCC 
AAGAACCTGA TCCTGGCTCC TGGGGAAGAT GGGAGGCTGG TATGCCGAGC CAATGGGAAC 
CCGAAGCCGA CCGTGCAGTG GATGGTGAAT GGAGAGCCTT TACAATCGGC ACCACCCAAT 
CCCAACCGTG AGGTAGCTGG AGACACTATC ATCTTCCGGG ATACTCAGAT CAGCAGCAGG 
GCAGTGTACC AATGTAATAC ATCCAATGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC 
AGCGTGTTAG ATGTACCCCC TCGGATGCTG TCTGCCCGCA ACCAGCTCAT CAGGGTGATC 
CTTTATAACC GGACACGGCT GGACTGTCCG TTCTTTGGGT CTCCCATCCC AACACTCCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGCGGTA ACTACCACGT CTACGAAAAC 
GGCAGTCTAG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC 
ACCAACATCC TGGGCAAAGC CGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACAGGA TGCCCGAGGA CCAGGTGGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC 
CGCGTGAAAC ATGACCCCTC CTTGAAGCTC ACAGTCTCCT GGCTGAAGGA CGATGAGCCA 
CTCTACATTG GAAACAGGAT GAAGAAGGAA GATGACTCCC TGACGATCTT CGGAGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTATG GCCAGCACCG AGCTGGACCA GGACCTGGCA 
AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTA 
CCCAAAGGGC GACCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT 
GTGAGGCTGA CCTGGATCCC AGGGGATGAC AACAACAGCC CTATCACAGA CTACGTCGTT 
CAGTTTGAAG AGGACCAGTT CCAACCAGGG GTGTGGCATG ACCACTCCAG GTTCCCAGGC 
AGCGTCAACT CAGCCGTCCT CCATCTGTCC CCATATGTCA ACTACCAATT CAGAGTCATC 
GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACCAGT 
GGGGCACCCC CTGAATCTAA TCCCAGTGAT GTGAAGGGCG AAGGGACAAG AAAGAACAAT 
ATGGAGATCA CGTGGACGCC TATGAATGCT ACCTCTGCCT TTGGCCCCAA CCTACGCTAC 
ATTGTCAAGT GGCGACGGAG AGAGACCCGA GAGACTTGGA ACAATGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACGCCTGTC TACGTTCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TTGGGAAAGG CCCCGAGCCT GACACCATCA TTGGGTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGAGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT 
TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG 
GCCAGCTTCC CTGGTGACCG TCCCCGGGGC GTGGTGGCCC GCCTGTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTGGT GGTCAATGGG AGAGGTGACG GGCCTCGAAG TGAAACCAAG 
GAATTCACCA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GGTTCAGAGT CCGACAGCCC 
AACCTGGAGA CCATCAACCT GGAGTGGGAC CACCCAGAGC ACCCCAACGG AATCCTGATT 
GGATACATCC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCTCT GTGCAGAGAG CAGACCCAGT GTCGCGTTAC 
CGCTTCTCCC TCAGTGCCAG GACACAGGTG GGCTCTGGAG AAGCAGCCAC AGAGGAGTCC 
CCAGCACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACGTTGCC CCCGACTACT 
GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA 
GCCACAACAG TTCCCATCAT TCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC 
ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CTACGGAGAG CCCTCCCACT 
ACCACTGCTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC 
ACAGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA 
ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG 
GCCCAGGCCC AGCCTATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG 
GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA 
GCTTACACCA ATAACCAGGC AGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT 
GCCATTGCCC TTCTGGTGCT GATCCTTCTC ATCGTCTGCT TCATCAAGAG GAGTCGAGGT 
GGCAAGTACC CAGTGCGGGA AAAGAAGGAT GTCCCCTTGG GTCCTGAAGA CCCCAAAGAA 
GAAGATGGCT CATTTGACTA CAGTGATGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA 
TCTCTGGATG GCACCATCAA GCAGCAGGAG AGCGATGACA GCCTGGTGGA CTATGGCGAA 
GGCGGCGAGG GCCAGTTCAA TGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAAAAAG 
GACAAGGAGG AAACGGAGGG CAATGAGAGC TCAGAGGCCA CATCACCAGT CAATGCCATC 
TATTCCCTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248019.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246321.1 Mus musculus neurofascin (Nfasc), transcript variant X21, mRNA.

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CloneID	OMu74382
Clone ID Related Accession (Same CDS sequence)	XM_011248024.2 , XM_011248023.1 , XM_011248019.1 , XM_011248024.3
Accession Version	XM_011248019.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACTATCA CCAAGCAGTC CGTGAAGGAC CACATCGTGG ACCCTCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG CAACCCCGCC CCCAGTTTTC ACTGGACTCG CAACAGCAGA 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGATCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAATACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TTGGCACTGC ACTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTGACACTA 
CAGTGCAACC CCCCACCCGG CCTCCCGTCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ACGCGCGCTT TCACTTCACC 
CACACCATTC AGCAGAAGAA CCCCTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC AGAAAGAACG 
CCCAGCTTCA TGTATCCCCA GGGCACATCG AGCAGTCAGA TGGTTCTCCG TGGCATGGAC 
CTGCTGCTGG AATGCATTGC CTCTGGCGTC CCAACACCAG ACATTGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTAA CAAGGCCAAG TTCGAGAACT TTAATAAGGC TCTGCGCATC 
ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CATACTGGCT GGATGAGCCC 
AAGAACCTGA TCCTGGCTCC TGGGGAAGAT GGGAGGCTGG TATGCCGAGC CAATGGGAAC 
CCGAAGCCGA CCGTGCAGTG GATGGTGAAT GGAGAGCCTT TACAATCGGC ACCACCCAAT 
CCCAACCGTG AGGTAGCTGG AGACACTATC ATCTTCCGGG ATACTCAGAT CAGCAGCAGG 
GCAGTGTACC AATGTAATAC ATCCAATGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC 
AGCGTGTTAG ATGTACCCCC TCGGATGCTG TCTGCCCGCA ACCAGCTCAT CAGGGTGATC 
CTTTATAACC GGACACGGCT GGACTGTCCG TTCTTTGGGT CTCCCATCCC AACACTCCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGCGGTA ACTACCACGT CTACGAAAAC 
GGCAGTCTAG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC 
ACCAACATCC TGGGCAAAGC CGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACAGGA TGCCCGAGGA CCAGGTGGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC 
CGCGTGAAAC ATGACCCCTC CTTGAAGCTC ACAGTCTCCT GGCTGAAGGA CGATGAGCCA 
CTCTACATTG GAAACAGGAT GAAGAAGGAA GATGACTCCC TGACGATCTT CGGAGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTATG GCCAGCACCG AGCTGGACCA GGACCTGGCA 
AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTA 
CCCAAAGGGC GACCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT 
GTGAGGCTGA CCTGGATCCC AGGGGATGAC AACAACAGCC CTATCACAGA CTACGTCGTT 
CAGTTTGAAG AGGACCAGTT CCAACCAGGG GTGTGGCATG ACCACTCCAG GTTCCCAGGC 
AGCGTCAACT CAGCCGTCCT CCATCTGTCC CCATATGTCA ACTACCAATT CAGAGTCATC 
GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACCAGT 
GGGGCACCCC CTGAATCTAA TCCCAGTGAT GTGAAGGGCG AAGGGACAAG AAAGAACAAT 
ATGGAGATCA CGTGGACGCC TATGAATGCT ACCTCTGCCT TTGGCCCCAA CCTACGCTAC 
ATTGTCAAGT GGCGACGGAG AGAGACCCGA GAGACTTGGA ACAATGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACGCCTGTC TACGTTCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TTGGGAAAGG CCCCGAGCCT GACACCATCA TTGGGTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGAGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT 
TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG 
GCCAGCTTCC CTGGTGACCG TCCCCGGGGC GTGGTGGCCC GCCTGTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTGGT GGTCAATGGG AGAGGTGACG GGCCTCGAAG TGAAACCAAG 
GAATTCACCA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GGTTCAGAGT CCGACAGCCC 
AACCTGGAGA CCATCAACCT GGAGTGGGAC CACCCAGAGC ACCCCAACGG AATCCTGATT 
GGATACATCC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCTCT GTGCAGAGAG CAGACCCAGT GTCGCGTTAC 
CGCTTCTCCC TCAGTGCCAG GACACAGGTG GGCTCTGGAG AAGCAGCCAC AGAGGAGTCC 
CCAGCACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACGTTGCC CCCGACTACT 
GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA 
GCCACAACAG TTCCCATCAT TCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC 
ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CTACGGAGAG CCCTCCCACT 
ACCACTGCTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC 
ACAGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA 
ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG 
GCCCAGGCCC AGCCTATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG 
GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA 
GCTTACACCA ATAACCAGGC AGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT 
GCCATTGCCC TTCTGGTGCT GATCCTTCTC ATCGTCTGCT TCATCAAGAG GAGTCGAGGT 
GGCAAGTACC CAGTGCGGGA AAAGAAGGAT GTCCCCTTGG GTCCTGAAGA CCCCAAAGAA 
GAAGATGGCT CATTTGACTA CAGTGATGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA 
TCTCTGGATG GCACCATCAA GCAGCAGGAG AGCGATGACA GCCTGGTGGA CTATGGCGAA 
GGCGGCGAGG GCCAGTTCAA TGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAAAAAG 
GACAAGGAGG AAACGGAGGG CAATGAGAGC TCAGAGGCCA CATCACCAGT CAATGCCATC 
TATTCCCTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246321.1
CDS	312..4406
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248019.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248019.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACTATCA CCAAGCAGTC CGTGAAGGAC CACATCGTGG ACCCTCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG CAACCCCGCC CCCAGTTTTC ACTGGACTCG CAACAGCAGA 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGATCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAATACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TTGGCACTGC ACTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTGACACTA 
CAGTGCAACC CCCCACCCGG CCTCCCGTCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ACGCGCGCTT TCACTTCACC 
CACACCATTC AGCAGAAGAA CCCCTTCACC CTCAAGGTCC TCACCAACAA CCCCTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC AGAAAGAACG 
CCCAGCTTCA TGTATCCCCA GGGCACATCG AGCAGTCAGA TGGTTCTCCG TGGCATGGAC 
CTGCTGCTGG AATGCATTGC CTCTGGCGTC CCAACACCAG ACATTGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTAA CAAGGCCAAG TTCGAGAACT TTAATAAGGC TCTGCGCATC 
ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CATACTGGCT GGATGAGCCC 
AAGAACCTGA TCCTGGCTCC TGGGGAAGAT GGGAGGCTGG TATGCCGAGC CAATGGGAAC 
CCGAAGCCGA CCGTGCAGTG GATGGTGAAT GGAGAGCCTT TACAATCGGC ACCACCCAAT 
CCCAACCGTG AGGTAGCTGG AGACACTATC ATCTTCCGGG ATACTCAGAT CAGCAGCAGG 
GCAGTGTACC AATGTAATAC ATCCAATGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC 
AGCGTGTTAG ATGTACCCCC TCGGATGCTG TCTGCCCGCA ACCAGCTCAT CAGGGTGATC 
CTTTATAACC GGACACGGCT GGACTGTCCG TTCTTTGGGT CTCCCATCCC AACACTCCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGCGGTA ACTACCACGT CTACGAAAAC 
GGCAGTCTAG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC 
ACCAACATCC TGGGCAAAGC CGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACAGGA TGCCCGAGGA CCAGGTGGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC 
CGCGTGAAAC ATGACCCCTC CTTGAAGCTC ACAGTCTCCT GGCTGAAGGA CGATGAGCCA 
CTCTACATTG GAAACAGGAT GAAGAAGGAA GATGACTCCC TGACGATCTT CGGAGTGGCA 
GAGCGGGACC AGGGCAGTTA CACGTGTATG GCCAGCACCG AGCTGGACCA GGACCTGGCA 
AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCAA CTAACCGTTT GGCTGCCCTA 
CCCAAAGGGC GACCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT 
GTGAGGCTGA CCTGGATCCC AGGGGATGAC AACAACAGCC CTATCACAGA CTACGTCGTT 
CAGTTTGAAG AGGACCAGTT CCAACCAGGG GTGTGGCATG ACCACTCCAG GTTCCCAGGC 
AGCGTCAACT CAGCCGTCCT CCATCTGTCC CCATATGTCA ACTACCAATT CAGAGTCATC 
GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACCAGT 
GGGGCACCCC CTGAATCTAA TCCCAGTGAT GTGAAGGGCG AAGGGACAAG AAAGAACAAT 
ATGGAGATCA CGTGGACGCC TATGAATGCT ACCTCTGCCT TTGGCCCCAA CCTACGCTAC 
ATTGTCAAGT GGCGACGGAG AGAGACCCGA GAGACTTGGA ACAATGTCAC AGTGTGGGGC 
TCTCGCTACG TGGTGGGGCA GACGCCTGTC TACGTTCCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TTGGGAAAGG CCCCGAGCCT GACACCATCA TTGGGTACTC CGGAGAAGAT 
TATCCCAGGG CTGCGCCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGAGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT 
TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG 
GCCAGCTTCC CTGGTGACCG TCCCCGGGGC GTGGTGGCCC GCCTGTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTGGT GGTCAATGGG AGAGGTGACG GGCCTCGAAG TGAAACCAAG 
GAATTCACCA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GGTTCAGAGT CCGACAGCCC 
AACCTGGAGA CCATCAACCT GGAGTGGGAC CACCCAGAGC ACCCCAACGG AATCCTGATT 
GGATACATCC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCTCT GTGCAGAGAG CAGACCCAGT GTCGCGTTAC 
CGCTTCTCCC TCAGTGCCAG GACACAGGTG GGCTCTGGAG AAGCAGCCAC AGAGGAGTCC 
CCAGCACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACGTTGCC CCCGACTACT 
GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA 
GCCACAACAG TTCCCATCAT TCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC 
ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CTACGGAGAG CCCTCCCACT 
ACCACTGCTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC 
ACAGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA 
ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG 
GCCCAGGCCC AGCCTATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG 
GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA 
GCTTACACCA ATAACCAGGC AGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT 
GCCATTGCCC TTCTGGTGCT GATCCTTCTC ATCGTCTGCT TCATCAAGAG GAGTCGAGGT 
GGCAAGTACC CAGTGCGGGA AAAGAAGGAT GTCCCCTTGG GTCCTGAAGA CCCCAAAGAA 
GAAGATGGCT CATTTGACTA CAGTGATGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA 
TCTCTGGATG GCACCATCAA GCAGCAGGAG AGCGATGACA GCCTGGTGGA CTATGGCGAA 
GGCGGCGAGG GCCAGTTCAA TGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAAAAAG 
GACAAGGAGG AAACGGAGGG CAATGAGAGC TCAGAGGCCA CATCACCAGT CAATGCCATC 
TATTCCCTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248020.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246322.1 Mus musculus neurofascin (Nfasc), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OMu74383
Clone ID Related Accession (Same CDS sequence)	XM_011248020.2 , XM_011248021.1 , XM_030254547.1 , XM_011248020.3
Accession Version	XM_011248020.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248020.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TACCCAAAGG GCGACCAGAC 
CGACCCAGGG ACCTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC 
CCAGGGGATG ACAACAACAG CCCTATCACA GACTACGTCG TTCAGTTTGA AGAGGACCAG 
TTCCAACCAG GGGTGTGGCA TGACCACTCC AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC 
CTCCATCTGT CCCCATATGT CAACTACCAA TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTTCC ATCCGAGCGG TACCGAACCA GTGGGGCACC CCCTGAATCT 
AATCCCAGTG ATGTGAAGGG CGAAGGGACA AGAAAGAACA ATATGGAGAT CACGTGGACG 
CCTATGAATG CTACCTCTGC CTTTGGCCCC AACCTACGCT ACATTGTCAA GTGGCGACGG 
AGAGAGACCC GAGAGACTTG GAACAATGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACGCCTG TCTACGTTCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTTGGGAAA 
GGCCCCGAGC CTGACACCAT CATTGGGTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGAGTC 
TACTCTGACA CGGTCCAGGG CCAGCTCAGA GAGTATCGAG CTTACTACTG GAGGGAAAGC 
AGTTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AGGCCAGCTT CCCTGGTGAC 
CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC CCCTACAGTA ACTACAAGCT GGAGATGGTG 
GTGGTCAATG GGAGAGGTGA CGGGCCTCGA AGTGAAACCA AGGAATTCAC CACCCCAGAA 
GGAGTACCCA GTGCCCCCAG GCGGTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC 
CTGGAGTGGG ACCACCCAGA GCACCCCAAC GGAATCCTGA TTGGATACAT CCTCAGATAC 
GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCT CTGTGCAGAG AGCAGACCCA GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC 
AGGACACAGG TGGGCTCTGG AGAAGCAGCC ACAGAGGAGT CCCCAGCACC TCCAAATGAA 
GCTACTCCAA CTGCAGCTCC TCCCACGTTG CCCCCGACTA CTGTGGGTAC CACAGGCCTT 
GTGAGCAGTA CTGATGCTAC TGCCCTTGCT GCCACCAGTG AAGCCACAAC AGTTCCCATC 
ATTCCAACCG TCGTACCTAC CACCGTCGCC ACCACCATTG CCACAACTAC TACAACCACT 
GCCGCCACCA CCACCACCAC CACTACGGAG AGCCCTCCCA CTACCACTGC TGGGACTAAG 
ATTCACGAAA CCGCCCCCGA CGAGCAGTCC ATTTGGAACG TCACAGTGCT CCCCAACAGT 
AAATGGGCCA ACATCACCTG GAAGCACAAT TTCAGGCCTG GAACTGACTT TGTGGTTGAG 
TACATCGACA GCAACCATAC GAAAAAAACT GTCCCTGTTA AGGCCCAGGC CCAGCCTATA 
CAGCTGACAG ACCTCTTTCC CGGGATGACG TACACGTTGC GGGTGTATTC CCGGGACAAC 
GAGGGCATCA GCAGTACCGT CATCACCTTT ATGACCAGTA CAGCTTACAC CAATAACCAG 
GCAGACATCG CCACCCAGGG CTGGTTCATC GGGCTCATGT GTGCCATTGC CCTTCTGGTG 
CTGATCCTTC TCATCGTCTG CTTCATCAAG AGGAGTCGAG GTGGCAAGTA CCCAGTGCGG 
GAAAAGAAGG ATGTCCCCTT GGGTCCTGAA GACCCCAAAG AAGAAGATGG CTCATTTGAC 
TACAGTGATG AGGACAACAA GCCCCTGCAG GGCAGCCAGA CATCTCTGGA TGGCACCATC 
AAGCAGCAGG AGAGCGATGA CAGCCTGGTG GACTATGGCG AAGGCGGCGA GGGCCAGTTC 
AATGAAGATG GCTCCTTTAT TGGCCAGTAC ACTGTCAAAA AGGACAAGGA GGAAACGGAG 
GGCAATGAGA GCTCAGAGGC CACATCACCA GTCAATGCCA TCTATTCCCT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246322.1
CDS	541..4617
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248020.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248020.2

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TACCCAAAGG GCGACCAGAC 
CGACCCAGGG ACCTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC 
CCAGGGGATG ACAACAACAG CCCTATCACA GACTACGTCG TTCAGTTTGA AGAGGACCAG 
TTCCAACCAG GGGTGTGGCA TGACCACTCC AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC 
CTCCATCTGT CCCCATATGT CAACTACCAA TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTTCC ATCCGAGCGG TACCGAACCA GTGGGGCACC CCCTGAATCT 
AATCCCAGTG ATGTGAAGGG CGAAGGGACA AGAAAGAACA ATATGGAGAT CACGTGGACG 
CCTATGAATG CTACCTCTGC CTTTGGCCCC AACCTACGCT ACATTGTCAA GTGGCGACGG 
AGAGAGACCC GAGAGACTTG GAACAATGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACGCCTG TCTACGTTCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTTGGGAAA 
GGCCCCGAGC CTGACACCAT CATTGGGTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGAGTC 
TACTCTGACA CGGTCCAGGG CCAGCTCAGA GAGTATCGAG CTTACTACTG GAGGGAAAGC 
AGTTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AGGCCAGCTT CCCTGGTGAC 
CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC CCCTACAGTA ACTACAAGCT GGAGATGGTG 
GTGGTCAATG GGAGAGGTGA CGGGCCTCGA AGTGAAACCA AGGAATTCAC CACCCCAGAA 
GGAGTACCCA GTGCCCCCAG GCGGTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC 
CTGGAGTGGG ACCACCCAGA GCACCCCAAC GGAATCCTGA TTGGATACAT CCTCAGATAC 
GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCT CTGTGCAGAG AGCAGACCCA GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC 
AGGACACAGG TGGGCTCTGG AGAAGCAGCC ACAGAGGAGT CCCCAGCACC TCCAAATGAA 
GCTACTCCAA CTGCAGCTCC TCCCACGTTG CCCCCGACTA CTGTGGGTAC CACAGGCCTT 
GTGAGCAGTA CTGATGCTAC TGCCCTTGCT GCCACCAGTG AAGCCACAAC AGTTCCCATC 
ATTCCAACCG TCGTACCTAC CACCGTCGCC ACCACCATTG CCACAACTAC TACAACCACT 
GCCGCCACCA CCACCACCAC CACTACGGAG AGCCCTCCCA CTACCACTGC TGGGACTAAG 
ATTCACGAAA CCGCCCCCGA CGAGCAGTCC ATTTGGAACG TCACAGTGCT CCCCAACAGT 
AAATGGGCCA ACATCACCTG GAAGCACAAT TTCAGGCCTG GAACTGACTT TGTGGTTGAG 
TACATCGACA GCAACCATAC GAAAAAAACT GTCCCTGTTA AGGCCCAGGC CCAGCCTATA 
CAGCTGACAG ACCTCTTTCC CGGGATGACG TACACGTTGC GGGTGTATTC CCGGGACAAC 
GAGGGCATCA GCAGTACCGT CATCACCTTT ATGACCAGTA CAGCTTACAC CAATAACCAG 
GCAGACATCG CCACCCAGGG CTGGTTCATC GGGCTCATGT GTGCCATTGC CCTTCTGGTG 
CTGATCCTTC TCATCGTCTG CTTCATCAAG AGGAGTCGAG GTGGCAAGTA CCCAGTGCGG 
GAAAAGAAGG ATGTCCCCTT GGGTCCTGAA GACCCCAAAG AAGAAGATGG CTCATTTGAC 
TACAGTGATG AGGACAACAA GCCCCTGCAG GGCAGCCAGA CATCTCTGGA TGGCACCATC 
AAGCAGCAGG AGAGCGATGA CAGCCTGGTG GACTATGGCG AAGGCGGCGA GGGCCAGTTC 
AATGAAGATG GCTCCTTTAT TGGCCAGTAC ACTGTCAAAA AGGACAAGGA GGAAACGGAG 
GGCAATGAGA GCTCAGAGGC CACATCACCA GTCAATGCCA TCTATTCCCT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_011248021.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246323.1 Mus musculus neurofascin (Nfasc), transcript variant X25, mRNA.

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CloneID	OMu74383
Clone ID Related Accession (Same CDS sequence)	XM_011248020.2 , XM_011248021.1 , XM_030254547.1 , XM_011248020.3
Accession Version	XM_011248021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurofascin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TACCCAAAGG GCGACCAGAC 
CGACCCAGGG ACCTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC 
CCAGGGGATG ACAACAACAG CCCTATCACA GACTACGTCG TTCAGTTTGA AGAGGACCAG 
TTCCAACCAG GGGTGTGGCA TGACCACTCC AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC 
CTCCATCTGT CCCCATATGT CAACTACCAA TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTTCC ATCCGAGCGG TACCGAACCA GTGGGGCACC CCCTGAATCT 
AATCCCAGTG ATGTGAAGGG CGAAGGGACA AGAAAGAACA ATATGGAGAT CACGTGGACG 
CCTATGAATG CTACCTCTGC CTTTGGCCCC AACCTACGCT ACATTGTCAA GTGGCGACGG 
AGAGAGACCC GAGAGACTTG GAACAATGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACGCCTG TCTACGTTCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTTGGGAAA 
GGCCCCGAGC CTGACACCAT CATTGGGTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGAGTC 
TACTCTGACA CGGTCCAGGG CCAGCTCAGA GAGTATCGAG CTTACTACTG GAGGGAAAGC 
AGTTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AGGCCAGCTT CCCTGGTGAC 
CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC CCCTACAGTA ACTACAAGCT GGAGATGGTG 
GTGGTCAATG GGAGAGGTGA CGGGCCTCGA AGTGAAACCA AGGAATTCAC CACCCCAGAA 
GGAGTACCCA GTGCCCCCAG GCGGTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC 
CTGGAGTGGG ACCACCCAGA GCACCCCAAC GGAATCCTGA TTGGATACAT CCTCAGATAC 
GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCT CTGTGCAGAG AGCAGACCCA GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC 
AGGACACAGG TGGGCTCTGG AGAAGCAGCC ACAGAGGAGT CCCCAGCACC TCCAAATGAA 
GCTACTCCAA CTGCAGCTCC TCCCACGTTG CCCCCGACTA CTGTGGGTAC CACAGGCCTT 
GTGAGCAGTA CTGATGCTAC TGCCCTTGCT GCCACCAGTG AAGCCACAAC AGTTCCCATC 
ATTCCAACCG TCGTACCTAC CACCGTCGCC ACCACCATTG CCACAACTAC TACAACCACT 
GCCGCCACCA CCACCACCAC CACTACGGAG AGCCCTCCCA CTACCACTGC TGGGACTAAG 
ATTCACGAAA CCGCCCCCGA CGAGCAGTCC ATTTGGAACG TCACAGTGCT CCCCAACAGT 
AAATGGGCCA ACATCACCTG GAAGCACAAT TTCAGGCCTG GAACTGACTT TGTGGTTGAG 
TACATCGACA GCAACCATAC GAAAAAAACT GTCCCTGTTA AGGCCCAGGC CCAGCCTATA 
CAGCTGACAG ACCTCTTTCC CGGGATGACG TACACGTTGC GGGTGTATTC CCGGGACAAC 
GAGGGCATCA GCAGTACCGT CATCACCTTT ATGACCAGTA CAGCTTACAC CAATAACCAG 
GCAGACATCG CCACCCAGGG CTGGTTCATC GGGCTCATGT GTGCCATTGC CCTTCTGGTG 
CTGATCCTTC TCATCGTCTG CTTCATCAAG AGGAGTCGAG GTGGCAAGTA CCCAGTGCGG 
GAAAAGAAGG ATGTCCCCTT GGGTCCTGAA GACCCCAAAG AAGAAGATGG CTCATTTGAC 
TACAGTGATG AGGACAACAA GCCCCTGCAG GGCAGCCAGA CATCTCTGGA TGGCACCATC 
AAGCAGCAGG AGAGCGATGA CAGCCTGGTG GACTATGGCG AAGGCGGCGA GGGCCAGTTC 
AATGAAGATG GCTCCTTTAT TGGCCAGTAC ACTGTCAAAA AGGACAAGGA GGAAACGGAG 
GGCAATGAGA GCTCAGAGGC CACATCACCA GTCAATGCCA TCTATTCCCT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246323.1
CDS	212..4288
Translation	MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAATTTTTTTESPPTTTAGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_011248021.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurofascin (Nfasc), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248021.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACATAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATCGAGAT TCCGATGGAC CTGACCCAAC CCCCAACTAT CACCAAGCAG 
TCCGTGAAGG ACCACATCGT GGACCCTCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGCAACCCCG CCCCCAGTTT TCACTGGACT CGCAACAGCA GATTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGATCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAATACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTTGGCACT 
GCACTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTGACAC TACAGTGCAA CCCCCCACCC 
GGCCTCCCGT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAACGCGCGC TTTCACTTCA CCCACACCAT TCAGCAGAAG 
AACCCCTTCA CCCTCAAGGT CCTCACCAAC AACCCCTATA ATGACTCGTC CTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCAGAAAGAA CGCCCAGCTT CATGTATCCC 
CAGGGCACAT CGAGCAGTCA GATGGTTCTC CGTGGCATGG ACCTGCTGCT GGAATGCATT 
GCCTCTGGCG TCCCAACACC AGACATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
AACAAGGCCA AGTTCGAGAA CTTTAATAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC TCCATACTGG CTGGATGAGC CCAAGAACCT GATCCTGGCT 
CCTGGGGAAG ATGGGAGGCT GGTATGCCGA GCCAATGGGA ACCCGAAGCC GACCGTGCAG 
TGGATGGTGA ATGGAGAGCC TTTACAATCG GCACCACCCA ATCCCAACCG TGAGGTAGCT 
GGAGACACTA TCATCTTCCG GGATACTCAG ATCAGCAGCA GGGCAGTGTA CCAATGTAAT 
ACATCCAATG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTGTT AGATGTACCC 
CCTCGGATGC TGTCTGCCCG CAACCAGCTC ATCAGGGTGA TCCTTTATAA CCGGACACGG 
CTGGACTGTC CGTTCTTTGG GTCTCCCATC CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGCGG TAACTACCAC GTCTACGAAA ACGGCAGTCT AGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGCAAA 
GCCGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCCGAG 
GACCAGGTGG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGCGTGAA ACATGACCCC 
TCCTTGAAGC TCACAGTCTC CTGGCTGAAG GACGATGAGC CACTCTACAT TGGAAACAGG 
ATGAAGAAGG AAGATGACTC CCTGACGATC TTCGGAGTGG CAGAGCGGGA CCAGGGCAGT 
TACACGTGTA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGCTGATC AGGCCACTCC AACTAACCGT TTGGCTGCCC TACCCAAAGG GCGACCAGAC 
CGACCCAGGG ACCTGGAGCT CACTGACCTG GCTGAGAGGA GTGTGAGGCT GACCTGGATC 
CCAGGGGATG ACAACAACAG CCCTATCACA GACTACGTCG TTCAGTTTGA AGAGGACCAG 
TTCCAACCAG GGGTGTGGCA TGACCACTCC AGGTTCCCAG GCAGCGTCAA CTCAGCCGTC 
CTCCATCTGT CCCCATATGT CAACTACCAA TTCAGAGTCA TCGCTGTCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTTCC ATCCGAGCGG TACCGAACCA GTGGGGCACC CCCTGAATCT 
AATCCCAGTG ATGTGAAGGG CGAAGGGACA AGAAAGAACA ATATGGAGAT CACGTGGACG 
CCTATGAATG CTACCTCTGC CTTTGGCCCC AACCTACGCT ACATTGTCAA GTGGCGACGG 
AGAGAGACCC GAGAGACTTG GAACAATGTC ACAGTGTGGG GCTCTCGCTA CGTGGTGGGG 
CAGACGCCTG TCTACGTTCC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTTGGGAAA 
GGCCCCGAGC CTGACACCAT CATTGGGTAC TCCGGAGAAG ATTATCCCAG GGCTGCGCCC 
ACTGAAGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGAGTC 
TACTCTGACA CGGTCCAGGG CCAGCTCAGA GAGTATCGAG CTTACTACTG GAGGGAAAGC 
AGTTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AGGCCAGCTT CCCTGGTGAC 
CGTCCCCGGG GCGTGGTGGC CCGCCTGTTC CCCTACAGTA ACTACAAGCT GGAGATGGTG 
GTGGTCAATG GGAGAGGTGA CGGGCCTCGA AGTGAAACCA AGGAATTCAC CACCCCAGAA 
GGAGTACCCA GTGCCCCCAG GCGGTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC 
CTGGAGTGGG ACCACCCAGA GCACCCCAAC GGAATCCTGA TTGGATACAT CCTCAGATAC 
GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCT CTGTGCAGAG AGCAGACCCA GTGTCGCGTT ACCGCTTCTC CCTCAGTGCC 
AGGACACAGG TGGGCTCTGG AGAAGCAGCC ACAGAGGAGT CCCCAGCACC TCCAAATGAA 
GCTACTCCAA CTGCAGCTCC TCCCACGTTG CCCCCGACTA CTGTGGGTAC CACAGGCCTT 
GTGAGCAGTA CTGATGCTAC TGCCCTTGCT GCCACCAGTG AAGCCACAAC AGTTCCCATC 
ATTCCAACCG TCGTACCTAC CACCGTCGCC ACCACCATTG CCACAACTAC TACAACCACT 
GCCGCCACCA CCACCACCAC CACTACGGAG AGCCCTCCCA CTACCACTGC TGGGACTAAG 
ATTCACGAAA CCGCCCCCGA CGAGCAGTCC ATTTGGAACG TCACAGTGCT CCCCAACAGT 
AAATGGGCCA ACATCACCTG GAAGCACAAT TTCAGGCCTG GAACTGACTT TGTGGTTGAG 
TACATCGACA GCAACCATAC GAAAAAAACT GTCCCTGTTA AGGCCCAGGC CCAGCCTATA 
CAGCTGACAG ACCTCTTTCC CGGGATGACG TACACGTTGC GGGTGTATTC CCGGGACAAC 
GAGGGCATCA GCAGTACCGT CATCACCTTT ATGACCAGTA CAGCTTACAC CAATAACCAG 
GCAGACATCG CCACCCAGGG CTGGTTCATC GGGCTCATGT GTGCCATTGC CCTTCTGGTG 
CTGATCCTTC TCATCGTCTG CTTCATCAAG AGGAGTCGAG GTGGCAAGTA CCCAGTGCGG 
GAAAAGAAGG ATGTCCCCTT GGGTCCTGAA GACCCCAAAG AAGAAGATGG CTCATTTGAC 
TACAGTGATG AGGACAACAA GCCCCTGCAG GGCAGCCAGA CATCTCTGGA TGGCACCATC 
AAGCAGCAGG AGAGCGATGA CAGCCTGGTG GACTATGGCG AAGGCGGCGA GGGCCAGTTC 
AATGAAGATG GCTCCTTTAT TGGCCAGTAC ACTGTCAAAA AGGACAAGGA GGAAACGGAG 
GGCAATGAGA GCTCAGAGGC CACATCACCA GTCAATGCCA TCTATTCCCT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The paranodal cytoskeleton clusters Na
eLife6(2017 Jan)
Amor V,Zhang C,Vainshtein A,Zhang A,Zollinger DR,Eshed-Eisenbach Y,Brophy PJ,Rasband MN,Peles E

Postnatal Loss of Neuronal and Glial Neurofascins Differentially Affects Node of Ranvier Maintenance and Myelinated Axon Function.
Frontiers in cellular neuroscience1111(2017)
Taylor AM,Saifetiarova J,Bhat MA

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Nfasc cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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