Mpg cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mpg
Entrez Gene ID	268395
Full Name	N-methylpurine-DNA glycosylase
Synonyms	9830006D05,AI326268,APNG,Aag,Mid1
General protein information	Preferred Names N-methylpurine-DNA glycosylase Names DNA-3-methyladenine glycosylase 3-alkyladenine DNA glycosylase 3-methyladenine DNA glycosidase 3-methyladenine DNA glycosylase ADPG alkylpurine-DNA-N-glycosylase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 A4\|11 18.83 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010822.3	NP_034952.2	DNA-3-methyladenine glycosylase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MPG	XP_005621833.1
Rattus norvegicus (Norway rat)	Mpg	NP_036733.2
Gallus gallus (chicken)	MPG	XP_414945.3
Danio rerio (zebrafish)	mpg	NP_001076319.1
Homo sapiens (human)	MPG	NP_001015052.1
	MPG	XP_001118427.2
Bos taurus (cattle)	MPG	XP_002683855.2
Mus musculus (house mouse)	Mpg	NP_034952.2
Xenopus tropicalis (tropical clawed frog)	LOC549932	NP_001017178.1

Gene Ontology
Bibliography

Related articles in PubMed

Roles of Aag, Alkbh2, and Alkbh3 in the Repair of Carboxymethylated and Ethylated Thymidine Lesions.
You C, Wang P, Nay SL, Wang J, Dai X, O'Connor TR, Wang Y
ACS chemical biology11(5)1332-8(2016 05)

DNA repair by MGMT, but not AAG, causes a threshold in alkylation-induced colorectal carcinogenesis.
Fahrer J, Frisch J, Nagel G, Kraus A, D?rsam B, Thomas AD, Rei?ig S, Waisman A, Kaina B
Carcinogenesis36(10)1235-44(2015 Oct)

Aag-initiated base excision repair promotes ischemia reperfusion injury in liver, brain, and kidney.
Ebrahimkhani MR, Daneshmand A, Mazumder A, Allocca M, Calvo JA, Abolhassani N, Jhun I, Muthupalani S, Ayata C, Samson LD
Proceedings of the National Academy of Sciences of the United States of America111(45)E4878-86(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The depletion of Aag did not significantly change the transcriptional inhibitory or mutagenic properties of all five examined lesions, suggesting a negligible role of Aag in the repair of these DNA adducts in mammalian cells.
Title: Roles of Aag, Alkbh2, and Alkbh3 in the Repair of Carboxymethylated and Ethylated Thymidine Lesions.

This study demonstrates for the first time a non-linear dose-response for alkylation-induced colorectal carcinogenesis and reveals DNA repair by MGMT, but not AAG, as a key node in determining a carcinogenic threshold.
Title: DNA repair by MGMT, but not AAG, causes a threshold in alkylation-induced colorectal carcinogenesis.

the detrimental effects of Aag-initiated BER during I/R and sterile inflammation, and present a novel target for controlling I/R-induced injury.
Title: Aag-initiated base excision repair promotes ischemia reperfusion injury in liver, brain, and kidney.

These results provide in vivo evidence that Aag-initiated BER may play a critical role in determining the side-effects of alkylating agent chemotherapies and that Parp1 plays a crucial role in Aag-mediated tissue damage.
Title: Aag DNA glycosylase promotes alkylation-induced tissue damage mediated by Parp1.

Toxicity, induced by tert-butyl-hydroperoxide and potassium bromate, differs in base excision repair proficient (Mpg (+/+), Nth1 (+/+)) and deficient (Mpg (-/-), Nth1 (-/-)) mouse embryonic fibroblasts following Msh2 knockdown, was examined.
Title: The effect of Msh2 knockdown on toxicity induced by tert-butyl-hydroperoxide, potassium bromate, and hydrogen peroxide in base excision repair proficient and deficient cells.

The following Mpg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mpg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15984	NM_010822.3 Latest version!	Mus musculus N-methylpurine-DNA glycosylase (Mpg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mpg ( NM_010822.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034952.2 Mus musculus N-methylpurine-DNA glycosylase (Mpg), mRNA.

Price & Availability↑

CloneID	OMu15984
Clone ID Related Accession (Same CDS sequence)	NM_010822.3
Accession Version	NM_010822.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	DNA-3-methyladenine glycosylase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY309585.1, AK018694.1 and AL929446.5. On Nov 17, 2009 this sequence version replaced NM_010822.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014754.1, AK156067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCCGCGC GCGGTGGTAG TGCGCGCCCG GGCAGAGGAG CCCTAAAACC GGTGTCCGTG 
ACCCTGCTCC CCGACACCGA GCAGCCTCCA TTTCTTGGAC GAGCCCGCCG CCCAGGGAAT 
GCCAGAGCAG GATCCCTAGT GACCGGATAT CATGAGGTCG GCCAGATGCC AGCGCCGCTT 
TCCCGAAAGA TTGGGCAAAA AAAGCAGCGA CTGGCAGATT CAGAGCAGCA GCAGACCCCT 
AAAGAGAGAC TCCTGTCGAC CCCGGGCCTC CGGCGGAGTA TCTACTTCTC CAGCCCAGAG 
GACCATTCTG GCCGGCTAGG ACCAGAGTTT TTTGACCAGC CAGCAGTCAC CCTAGCCCGT 
GCATTTCTGG GACAGGTTCT TGTCCGGCGA CTCGCTGATG GAACAGAACT CCGTGGGCGC 
ATTGTGGAGA CTGAGGCATA CTTGGGGCCA GAAGATGAAG CTGCTCACTC AAGAGGTGGC 
CGGCAGACCC CCCGGAACCG TGGCATGTTC ATGAAACCTG GGACCCTGTA TGTGTACCTC 
ATCTATGGCA TGTACTTCTG CTTGAATGTC TCTAGTCAAG GGGCTGGGGC TTGTGTCTTG 
CTAAGAGCAC TAGAGCCCCT GGAGGGCCTG GAGACCATGC GGCAGCTTCG TAACTCCCTC 
CGAAAAAGCA CTGTCGGCCG TTCCCTCAAG GACCGTGAAC TCTGTAGTGG TCCCTCCAAG 
CTGTGCCAGG CCCTGGCCAT TGATAAGAGC TTTGACCAGC GAGACCTGGC TCAAGATGAT 
GCTGTGTGGC TGGAGCATGG CCCTCTGGAG TCCAGTAGCC CAGCTGTGGT GGTGGCAGCA 
GCCCGTATAG GTATTGGTCA TGCAGGGGAG TGGACACAGA AGCCCTTGCG CTTCTATGTC 
CAGGGCAGCC CATGGGTCAG TGTGGTAGAC AGAGTGGCTG AACAGATGGA TCAGCCTCAG 
CAAACAGCCT GCTCTGAGGG GCTTTTAATT GTTCAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034952.2
CDS	37..1038
Translation	MPARGGSARPGRGALKPVSVTLLPDTEQPPFLGRARRPGNARAGSLVTGYHEVGQMPAPLSRKIGQKKQRLADSEQQQTPKERLLSTPGLRRSIYFSSPEDHSGRLGPEFFDQPAVTLARAFLGQVLVRRLADGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYLIYGMYFCLNVSSQGAGACVLLRALEPLEGLETMRQLRNSLRKSTVGRSLKDRELCSGPSKLCQALAIDKSFDQRDLAQDDAVWLEHGPLESSSPAVVVAAARIGIGHAGEWTQKPLRFYVQGSPWVSVVDRVAEQMDQPQQTACSEGLLIVQK

Target ORF information:

RefSeq Version	NM_010822.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-methylpurine-DNA glycosylase (Mpg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010822.3

ORF Insert Sequence:

ATGCCCGCGC GCGGTGGTAG TGCGCGCCCG GGCAGAGGAG CCCTAAAACC GGTGTCCGTG 
ACCCTGCTCC CCGACACCGA GCAGCCTCCA TTTCTTGGAC GAGCCCGCCG CCCAGGGAAT 
GCCAGAGCAG GATCCCTAGT GACCGGATAT CATGAGGTCG GCCAGATGCC AGCGCCGCTT 
TCCCGAAAGA TTGGGCAAAA AAAGCAGCGA CTGGCAGATT CAGAGCAGCA GCAGACCCCT 
AAAGAGAGAC TCCTGTCGAC CCCGGGCCTC CGGCGGAGTA TCTACTTCTC CAGCCCAGAG 
GACCATTCTG GCCGGCTAGG ACCAGAGTTT TTTGACCAGC CAGCAGTCAC CCTAGCCCGT 
GCATTTCTGG GACAGGTTCT TGTCCGGCGA CTCGCTGATG GAACAGAACT CCGTGGGCGC 
ATTGTGGAGA CTGAGGCATA CTTGGGGCCA GAAGATGAAG CTGCTCACTC AAGAGGTGGC 
CGGCAGACCC CCCGGAACCG TGGCATGTTC ATGAAACCTG GGACCCTGTA TGTGTACCTC 
ATCTATGGCA TGTACTTCTG CTTGAATGTC TCTAGTCAAG GGGCTGGGGC TTGTGTCTTG 
CTAAGAGCAC TAGAGCCCCT GGAGGGCCTG GAGACCATGC GGCAGCTTCG TAACTCCCTC 
CGAAAAAGCA CTGTCGGCCG TTCCCTCAAG GACCGTGAAC TCTGTAGTGG TCCCTCCAAG 
CTGTGCCAGG CCCTGGCCAT TGATAAGAGC TTTGACCAGC GAGACCTGGC TCAAGATGAT 
GCTGTGTGGC TGGAGCATGG CCCTCTGGAG TCCAGTAGCC CAGCTGTGGT GGTGGCAGCA 
GCCCGTATAG GTATTGGTCA TGCAGGGGAG TGGACACAGA AGCCCTTGCG CTTCTATGTC 
CAGGGCAGCC CATGGGTCAG TGTGGTAGAC AGAGTGGCTG AACAGATGGA TCAGCCTCAG 
CAAACAGCCT GCTCTGAGGG GCTTTTAATT GTTCAAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Roles of Aag, Alkbh2, and Alkbh3 in the Repair of Carboxymethylated and Ethylated Thymidine Lesions.
ACS chemical biology11(5)1332-8(2016 05)
You C,Wang P,Nay SL,Wang J,Dai X,O'Connor TR,Wang Y

DNA repair by MGMT, but not AAG, causes a threshold in alkylation-induced colorectal carcinogenesis.
Carcinogenesis36(10)1235-44(2015 Oct)
Fahrer J,Frisch J,Nagel G,Kraus A,D?rsam B,Thomas AD,Rei?ig S,Waisman A,Kaina B

Aag-initiated base excision repair promotes ischemia reperfusion injury in liver, brain, and kidney.
Proceedings of the National Academy of Sciences of the United States of America111(45)E4878-86(2014 Nov)
Ebrahimkhani MR,Daneshmand A,Mazumder A,Allocca M,Calvo JA,Abolhassani N,Jhun I,Muthupalani S,Ayata C,Samson LD

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Mpg cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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