Map3k2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Map3k2
Entrez Gene ID	26405
Full Name	mitogen-activated protein kinase kinase kinase 2
Synonyms	9630061B06Rik,AI585793,Mekk2,Mekk2b
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 2 Names mitogen-activated protein kinase kinase kinase 2 MAP/ERK kinase kinase 2 MAPK/ERK kinase kinase 2 MEK kinase 2 MEKK 2 protein kinase MEKK2b
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_011946.3	NP_036076.2	mitogen-activated protein kinase kinase kinase 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAP3K2	XP_001137344.1
Rattus norvegicus (Norway rat)	Map3k2	NP_612512.1
Gallus gallus (chicken)	MAP3K2	XP_004942945.1
Canis lupus familiaris (dog)	MAP3K2	XP_005631936.1
Mus musculus (house mouse)	Map3k2	NP_036076.2
Homo sapiens (human)	MAP3K2	NP_006600.3
Xenopus tropicalis (tropical clawed frog)	map3k2	XP_002942055.2
	MAP3K2	XP_002799467.1
Bos taurus (cattle)	MAP3K2	XP_002685278.2
Danio rerio (zebrafish)	map3k2	XP_686613.2

Gene Ontology
Bibliography

Related articles in PubMed

Upregulation of ?3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway.
Steinmetz CC, Tatavarty V, Sugino K, Shima Y, Joseph A, Lin H, Rutlin M, Lambo M, Hempel CM, Okaty BW, Paradis S, Nelson SB, Turrigiano GG
Cell reports16(10)2711-2722(2016 09)

Platelet-derived growth factor (PDGF)-induced activation of Erk5 MAP-kinase is dependent on Mekk2, Mek1/2, PKC and PI3-kinase, and affects BMP signaling.
Tsioumpekou M, Papadopoulos N, Burovic F, Heldin CH, Lennartsson J
Cellular signalling28(9)1422-31(2016 Sep)

MEKK2 mediates an alternative ?-catenin pathway that promotes bone formation.
Greenblatt MB, Shin DY, Oh H, Lee KY, Zhai B, Gygi SP, Lotinun S, Baron R, Liu D, Su B, Glimcher LH, Shim JH
Proceedings of the National Academy of Sciences of the United States of America113(9)E1226-35(2016 Mar)

Stk38 protein kinase preferentially inhibits TLR9-activated inflammatory responses by promoting MEKK2 ubiquitination in macrophages.
Wen M, Ma X, Cheng H, Jiang W, Xu X, Zhang Y, Zhang Y, Guo Z, Yu Y, Xu H, Qian C, Cao X, An H
Nature communications67167(2015 May)

MEKK2 regulates paxillin ubiquitylation and localization in MDA-MB 231 breast cancer cells.
Ameka M, Kahle MP, Perez-Neut M, Gentile S, Mirza AA, Cuevas BD
The Biochemical journal464(1)99-108(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Erk5 MAP kinase is activated in response to PDGF-BB in the smooth muscle cell line MOVAS in a manner dependent on Mekk2, Mek1/2, Mek5, PI3-kinase and protein kinase C (PKC).
Title: Platelet-derived growth factor (PDGF)-induced activation of Erk5 MAP-kinase is dependent on Mekk2, Mek1/2, PKC and PI3-kinase, and affects BMP signaling.

FGF2/MEKK2 pathway mediates an alternative nonclassical pathway for beta-catenin activation, and this pathway is a key regulator of bone formation by osteoblasts
Title: MEKK2 mediates an alternative ?-catenin pathway that promotes bone formation.

Stk38 protein kinase has a role in inhibiting TLR9-activated inflammatory responses by promoting MEKK2 ubiquitination in macrophages
Title: Stk38 protein kinase preferentially inhibits TLR9-activated inflammatory responses by promoting MEKK2 ubiquitination in macrophages.

methylation of MAP3K2 by SMYD3 increases MAP kinase signalling and promotes the formation of Ras-driven carcinomas
Title: SMYD3 links lysine methylation of MAP3K2 to Ras-driven cancer.

MEKK2 is regulated through a phosphorylation-dependent association with 14-3-3, a group of adapters that modulate dimerization and association between proteins
Title: MEKK2 kinase association with 14-3-3 protein regulates activation of c-Jun N-terminal kinase.

The following Map3k2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map3k2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21369	NM_011946.3 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 2 (Map3k2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map3k2 ( NM_011946.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036076.2 Mus musculus mitogen-activated protein kinase kinase kinase 2 (Map3k2), mRNA.

Price & Availability↑

CloneID	OMu21369
Clone ID Related Accession (Same CDS sequence)	NM_011946.3
Accession Version	NM_011946.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126686.3. On Mar 1, 2008 this sequence version replaced NM_011946.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U43186.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGATGATC AGCAAGCTTT GAATTCAATC ATGCAAGATT TGGCTGTCCT TCATAAGGCC 
AGTCGGCCAG CATTATCTTT ACAAGAAACC AGGAAAGCAA AACCTTCATC ACCAAAAAAA 
CAGAATGATG TTCGAGTCAA ATTTGAACAT AGAGGAGAAA AAAGGATCCT GCAGGTTACT 
AGACCAGTTA AACTAGAAGA CCTGAGATCT AAGTCTAAGA TCGCCTTTGG GCAGTCTATG 
GATCTACACT ATACCAACAA TGAGTTGGTA ATTCCGTTAA CTACCCAAGA TGACTTGGAC 
AAAGCTGTGG AACTGCTGGA TCGCAGTATT CACATGAAGA GTCTCAAGAT ATTACTTGTA 
GTAAATGGGA GTACACAGGC TACTAATTTA GAACCATCAC CGTCACCAGA AGATTTGAAT 
AATACACCAC TTGGTGCAGA GAGGAAAAAG CGGCTATCTG TAGTAGGTCC CCCTAATAGG 
GATAGAAGTT CCCCTCCTCC AGGATACATT CCAGACGAGC TACACCAGAT TGCCCGGAAT 
GGGTCATTCA CTAGCATCAA CAGTGAAGGA GAGTTCATTC CAGAGAGCAT GGACCAAATG 
CTGGATCCAT TGTCTTTAAG CAGCCCTGAA AATTCTGGCT CAGGAAGCTG TCCGTCACTT 
GATAGTCCTT TGGATGGAGA AAGCTACCCA AAATCACGGA TGCCTAGGGC ACAGAGCTAC 
CCAGATAATC ATCAGGAGTT TACAGACTAT GATAACCCCA TTTTTGAGAA ATTTGGAAAA 
GGAGGAACAT ATCCAAGAAG GTACCACGTT TCCTATCATC ACCAGGAGTA TAATGACGGT 
CGGAAGACTT TTCCAAGAGC TAGAAGGACC CAGGGCACCA GTTTCCGGTC TCCTGTGAGC 
TTCAGTCCTA CTGATCACTC CTTAAGCACT AGTAGTGGAA GCAGTGTCTT TACCCCAGAG 
TATGACGACA GTCGAATAAG AAGACGGGGG AGTGACATAG ACAATCCTAC TTTGACTGTC 
ACAGACATCA GCCCACCCAG CCGTTCACCT CGAGCTCCGA CCAACTGGAG ACTGGGCAAG 
CTGCTTGGCC AAGGAGCTTT TGGTAGGGTC TACCTCTGCT ATGATGTTGA TACCGGAAGA 
GAGCTGGCTG TTAAGCAAGT TCAGTTTAAC CCTGAGAGCC CAGAGACCAG CAAGGAAGTA 
AATGCACTTG AGTGTGAAAT TCAGTTGTTG AAAAACTTGT TGCATGAGCG AATTGTTCAG 
TATTATGGCT GTTTGAGGGA TCCTCAGGAG AAAACACTTT CCATCTTTAT GGAGTATATG 
CCAGGGGGTT CAATTAAGGA CCAACTAAAA GCCTACGGAG CTCTTACTGA GAACGTGACG 
AGGAAGTACA CCCGTCAGAT TCTGGAGGGG GTCCATTATT TGCATAGTAA TATGATTGTC 
CATAGAGATA TCAAAGGAGC AAATATCTTA AGGGATTCCA CAGGCAATAT CAAGTTAGGA 
GACTTTGGGG CTAGTAAACG GCTTCAGACC ATCTGTCTCT CAGGCACAGG AATGAAGTCT 
GTCACAGGCA CGCCATACTG GATGAGTCCT GAGGTCATCA GTGGAGAAGG CTATGGAAGA 
AAAGCAGACA TCTGGAGTGT AGCATGTACT GTGGTAGAAA TGCTAACTGA AAAGCCACCT 
TGGGCTGAAT TTGAAGCAAT GGCTGCCATC TTTAAGATCG CCACTCAGCC AACGAACCCA 
AAGCTGCCAC CTCATGTCTC AGACTATACT CGGGACTTCC TCAAACGGAT TTTTGTAGAG 
GCCAAACTTC GACCTTCAGC GGAGGAGCTC TTGCGGCACA TGTTTGTGCA TTATCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036076.2
CDS	529..2388
Translation	MDDQQALNSIMQDLAVLHKASRPALSLQETRKAKPSSPKKQNDVRVKFEHRGEKRILQVTRPVKLEDLRSKSKIAFGQSMDLHYTNNELVIPLTTQDDLDKAVELLDRSIHMKSLKILLVVNGSTQATNLEPSPSPEDLNNTPLGAERKKRLSVVGPPNRDRSSPPPGYIPDELHQIARNGSFTSINSEGEFIPESMDQMLDPLSLSSPENSGSGSCPSLDSPLDGESYPKSRMPRAQSYPDNHQEFTDYDNPIFEKFGKGGTYPRRYHVSYHHQEYNDGRKTFPRARRTQGTSFRSPVSFSPTDHSLSTSSGSSVFTPEYDDSRIRRRGSDIDNPTLTVTDISPPSRSPRAPTNWRLGKLLGQGAFGRVYLCYDVDTGRELAVKQVQFNPESPETSKEVNALECEIQLLKNLLHERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSTGNIKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPHVSDYTRDFLKRIFVEAKLRPSAEELLRHMFVHYH

Target ORF information:

RefSeq Version	NM_011946.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 2 (Map3k2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011946.3

ORF Insert Sequence:

ATGGATGATC AGCAAGCTTT GAATTCAATC ATGCAAGATT TGGCTGTCCT TCATAAGGCC 
AGTCGGCCAG CATTATCTTT ACAAGAAACC AGGAAAGCAA AACCTTCATC ACCAAAAAAA 
CAGAATGATG TTCGAGTCAA ATTTGAACAT AGAGGAGAAA AAAGGATCCT GCAGGTTACT 
AGACCAGTTA AACTAGAAGA CCTGAGATCT AAGTCTAAGA TCGCCTTTGG GCAGTCTATG 
GATCTACACT ATACCAACAA TGAGTTGGTA ATTCCGTTAA CTACCCAAGA TGACTTGGAC 
AAAGCTGTGG AACTGCTGGA TCGCAGTATT CACATGAAGA GTCTCAAGAT ATTACTTGTA 
GTAAATGGGA GTACACAGGC TACTAATTTA GAACCATCAC CGTCACCAGA AGATTTGAAT 
AATACACCAC TTGGTGCAGA GAGGAAAAAG CGGCTATCTG TAGTAGGTCC CCCTAATAGG 
GATAGAAGTT CCCCTCCTCC AGGATACATT CCAGACGAGC TACACCAGAT TGCCCGGAAT 
GGGTCATTCA CTAGCATCAA CAGTGAAGGA GAGTTCATTC CAGAGAGCAT GGACCAAATG 
CTGGATCCAT TGTCTTTAAG CAGCCCTGAA AATTCTGGCT CAGGAAGCTG TCCGTCACTT 
GATAGTCCTT TGGATGGAGA AAGCTACCCA AAATCACGGA TGCCTAGGGC ACAGAGCTAC 
CCAGATAATC ATCAGGAGTT TACAGACTAT GATAACCCCA TTTTTGAGAA ATTTGGAAAA 
GGAGGAACAT ATCCAAGAAG GTACCACGTT TCCTATCATC ACCAGGAGTA TAATGACGGT 
CGGAAGACTT TTCCAAGAGC TAGAAGGACC CAGGGCACCA GTTTCCGGTC TCCTGTGAGC 
TTCAGTCCTA CTGATCACTC CTTAAGCACT AGTAGTGGAA GCAGTGTCTT TACCCCAGAG 
TATGACGACA GTCGAATAAG AAGACGGGGG AGTGACATAG ACAATCCTAC TTTGACTGTC 
ACAGACATCA GCCCACCCAG CCGTTCACCT CGAGCTCCGA CCAACTGGAG ACTGGGCAAG 
CTGCTTGGCC AAGGAGCTTT TGGTAGGGTC TACCTCTGCT ATGATGTTGA TACCGGAAGA 
GAGCTGGCTG TTAAGCAAGT TCAGTTTAAC CCTGAGAGCC CAGAGACCAG CAAGGAAGTA 
AATGCACTTG AGTGTGAAAT TCAGTTGTTG AAAAACTTGT TGCATGAGCG AATTGTTCAG 
TATTATGGCT GTTTGAGGGA TCCTCAGGAG AAAACACTTT CCATCTTTAT GGAGTATATG 
CCAGGGGGTT CAATTAAGGA CCAACTAAAA GCCTACGGAG CTCTTACTGA GAACGTGACG 
AGGAAGTACA CCCGTCAGAT TCTGGAGGGG GTCCATTATT TGCATAGTAA TATGATTGTC 
CATAGAGATA TCAAAGGAGC AAATATCTTA AGGGATTCCA CAGGCAATAT CAAGTTAGGA 
GACTTTGGGG CTAGTAAACG GCTTCAGACC ATCTGTCTCT CAGGCACAGG AATGAAGTCT 
GTCACAGGCA CGCCATACTG GATGAGTCCT GAGGTCATCA GTGGAGAAGG CTATGGAAGA 
AAAGCAGACA TCTGGAGTGT AGCATGTACT GTGGTAGAAA TGCTAACTGA AAAGCCACCT 
TGGGCTGAAT TTGAAGCAAT GGCTGCCATC TTTAAGATCG CCACTCAGCC AACGAACCCA 
AAGCTGCCAC CTCATGTCTC AGACTATACT CGGGACTTCC TCAAACGGAT TTTTGTAGAG 
GCCAAACTTC GACCTTCAGC GGAGGAGCTC TTGCGGCACA TGTTTGTGCA TTATCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Upregulation of ?3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway.
Cell reports16(10)2711-2722(2016 09)
Steinmetz CC,Tatavarty V,Sugino K,Shima Y,Joseph A,Lin H,Rutlin M,Lambo M,Hempel CM,Okaty BW,Paradis S,Nelson SB,Turrigiano GG

Platelet-derived growth factor (PDGF)-induced activation of Erk5 MAP-kinase is dependent on Mekk2, Mek1/2, PKC and PI3-kinase, and affects BMP signaling.
Cellular signalling28(9)1422-31(2016 Sep)
Tsioumpekou M,Papadopoulos N,Burovic F,Heldin CH,Lennartsson J

MEKK2 mediates an alternative ?-catenin pathway that promotes bone formation.
Proceedings of the National Academy of Sciences of the United States of America113(9)E1226-35(2016 Mar)
Greenblatt MB,Shin DY,Oh H,Lee KY,Zhai B,Gygi SP,Lotinun S,Baron R,Liu D,Su B,Glimcher LH,Shim JH

Stk38 protein kinase preferentially inhibits TLR9-activated inflammatory responses by promoting MEKK2 ubiquitination in macrophages.
Nature communications67167(2015 May)
Wen M,Ma X,Cheng H,Jiang W,Xu X,Zhang Y,Zhang Y,Guo Z,Yu Y,Xu H,Qian C,Cao X,An H

MEKK2 regulates paxillin ubiquitylation and localization in MDA-MB 231 breast cancer cells.
The Biochemical journal464(1)99-108(2014 Nov)
Ameka M,Kahle MP,Perez-Neut M,Gentile S,Mirza AA,Cuevas BD

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Map3k2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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