Map3k1 cDNA ORF clone, Mus musculus(house mouse)

The following Map3k1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map3k1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu49052 XM_017315516.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu69202 XM_017315517.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu14906 NM_011945.3
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
View Old Accession Versions >>
OMu69202 XM_017315517.1 Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
OMu14906 NM_011945.2 Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OMu49052 XM_017315516.1 Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu69202
    Clone ID Related Accession (Same CDS sequence) XM_017315517.2 , XM_017315517.1
    Accession Version XM_017315517.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGAATA AAGAAACCCT CAAAGGACTG CACAAGATGG AGGATCGCCC GGAGGAGAGA 
    ATGATCCGGG AGAAGCTCAA GGCGACCTGT ATGCCCGCCT GGAAGCACGA GTGGTTGGAG
    AGGAGGAACA GGAGAGGCCC TGTGGTGGTG AAGCCAATCC CTATTAAAGG AGATGGATCT
    GAAATGAATA ACTTGGCAGC TGAGCCCCAA GGAGAGGGCC AGGCAGGTTC CGCTGCACCA
    GCCCCCAAGG GCCGACGAAG CCCATCTCCT GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG
    CCGGAATCCC CAGGAGTGAG ACGGAAACGA GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA
    ATCACACCAC CCCGAAGAGC CCCGTCACCG GATGGCTTCT CCCCGTACAG CCCAGAGGAG
    ACGAGCCGCC GCGTGAACAA AGTGATGAGA GCCAGGCTGT ACCTGCTGCA GCAGATAGGA
    CCCAACTCTT TCCTGATTGG AGGAGACAGT CCAGACAATA AATACCGGGT GTTTATTGGG
    CCACAGAACT GCAGCTGTGG GCGTGGAGCA TTCTGTATTC ACCTCTTGTT TGTCATGCTC
    CGGGTGTTTC AGCTAGAACC CTCTGACCCC ATGTTATGGA GAAAAACTTT AAAAAATTTC
    GAGGTTGAGA GTTTGTTCCA GAAATACCAC AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA
    TCCCGGAACA CCATCCAGAA GTTTGTGTCA CGCATGTCAA ATTGTCACAC ACTGTCATCG
    TCTAGCACAT CCACATCTAG TTCAGAAAAC AGCATCAAGG ATGAAGAGGA GCAGATGTGT
    CCCATCTGCT TGCTGGGCAT GCTGGATGAG GAGAGCCTGA CTGTGTGTGA AGATGGCTGC
    AGGAACAAGC TGCACCACCA TTGCATGTCC ATCTGGGCGG AAGAGTGTAG AAGAAATAGA
    GAGCCTTTAA TATGTCCCCT TTGTAGATCT AAGTGGAGAT CCCATGACTT CTACAGCCAT
    GAGTTATCAA GCCCCGTGGA GTCCCCCACC TCCCTGCGAG CTGTCCAGCA GCCATCCTCC
    CCACAGCAGC CCGTGGCCGG ATCACAGCGA AGGAATCAGG AGAGCAGTTT TAACCTTACT
    CATTTTGGAA CCCAGCAGAT TCCTTCCGCT TACAAAGATT TGGCCGAGCC ATGGATTCAG
    GTGTTTGGAA TGGAACTCGT TGGCTGCTTA TTCTCTAGAA ACTGGAACGT AAGGGAAATG
    GCCCTTAGGC GTCTTTCCCA CGACGTTAGT GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC
    ACTGGAAACT CTGGAGGCGG CAGTGGGGGC AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC
    TCCCAGCCCA GCATCTCAGG GGATGTGGTG GAGGCGTGCT GCAGTGTCCT GTCTATAGTC
    TGCGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACATTGAG AGCCATGCTG
    GTATACACTC CTTGCCACAG TCTGGCAGAA AGAATCAAAC TTCAGAGACT CCTCCGGCCA
    GTTGTAGACA CTATCCTTGT CAAGTGTGCA GATGCCAACA GCCGCACGAG TCAGCTGTCC
    ATATCTACAG TGCTGGAACT CTGCAAGGGC CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA
    ATACTTAAAG CTGGGTCCAT CGGGGTTGGT GGTGTCGATT ACGTCTTAAG TTGTATCCTT
    GGAAACCAAG CTGAATCAAA CAACTGGCAA GAACTGCTGG GTCGCCTCTG TCTTATAGAC
    AGGTTGCTGT TGGAATTTCC TGCTGAATTC TATCCTCATA TTGTCAGTAC TGATGTCTCA
    CAAGCTGAGC CTGTTGAAAT CAGGTACAAG AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG
    CAATCCATTG ACAATTCCCA CTCGATGGTT GGCAAGCTCT CTCGGAGGAT ATATCTGAGC
    TCTGCCAGGA TGGTGACCGC AGTGCCCGCT GTGTTTTCCA AGCTGGTAAC CATGCTTAAT
    GCTTCTGGCT CCACCCACTT CACCAGGATG CGCCGGCGTC TGATGGCTAT CGCGGATGAG
    GTAGAAATTG CCGAGGTCAT CCAGCTGGGT GTGGAGGACA CTGTGGATGG GCATCAGGAC
    AGCTTACAGG TGCTGGCCCC CGCCAGCTGT CTAGAAAACA GCTCCCTTGA GCACACAGTC
    CATAGAGAGA AAACTGGAAA AGGACTAAGT GCTACGAGAC TGAGTGCCAG CTCGGAGGAC
    ATTTCTGACA GACTGGCCGG CGTCTCTGTA GGACTTCCCA GCTCAACAAC AACAGAACAA
    CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA CCCCACAGTC AGTGTTTGAA CTCCTCCCCT
    TTGTCTCATG CTCAATTAAT GTTCCCAGCA CCATCAGCCC CTTGTTCCTC TGCCCCGTCT
    GTCCCAGATA TTTCTAAGCA CAGACCCCAG GCATTTGTTC CCTGCAAAAT ACCTTCCGCA
    TCTCCTCAGA CACAGCGCAA GTTCTCTCTA CAATTCCAGA GGAACTGCTC TGAACACCGA
    GACTCAGACC AGCTCTCCCC AGTCTTCACT CAGTCAAGGC CCCCACCCTC CAGTAACATA
    CACAGGCCAA AGCCATCCCG ACCCGTTCCG GGCAGTACAA GCAAACTAGG GGACGCCACA
    AAAAGTAGCA TGACACTTGA TCTGGGCAGT GCTTCCAGGT GTGACGACAG CTTTGGCGGC
    GGCGGCAACA GTGGCAACGC CGTCATACCC AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG
    GACAAGTGCA GGTTAGATGT GAACACCGAG CTCAACTCCA GCATCGAGGA CCTTCTTGAA
    GCATCCATGC CTTCAAGTGA CACGACAGTC ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT
    CCGGAAAAGG CCGAAAATGA CGACACCTAC AAAGACGACG TCAATCATAA TCAAAAGTGC
    AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG GCTTTAGCGA TCGCCATGGC GATGTCAGCG
    TCTCAGGATG CCCTCCCCAT CGTCCCTCAG CTGCAGGTGG AAAATGGAGA AGATATTATC
    ATCATTCAGC AGGACACACC AGAAACTCTT CCAGGACATA CCAAAGCGAA ACAGCCTTAC
    AGAGAAGACG CTGAGTGGCT GAAAGGCCAG CAGATAGGCC TCGGAGCATT TTCTTCCTGT
    TACCAAGCAC AGGATGTGGG GACTGGGACT TTAATGGCTG TGAAACAGGT GACGTACGTC
    AGAAACACAT CCTCCGAGCA GGAGGAGGTG GTGGAAGCGT TGAGGGAAGA GATCCGGATG
    ATGGGTCACC TCAACCATCC AAACATCATC CGGATGCTGG GGGCCACGTG CGAGAAGAGC
    AACTACAACC TCTTCATTGA GTGGATGGCG GGAGGATCTG TGGCTCACCT CTTGAGTAAA
    TACGGAGCTT TCAAGGAGTC AGTCGTCATT AACTACACTG AGCAGTTACT CCGTGGCCTT
    TCCTATCTCC ACGAGAACCA GATCATTCAC AGAGACGTCA AAGGTGCCAA CCTGCTCATT
    GACAGCACCG GTCAGAGGCT GAGAATTGCA GACTTTGGAG CTGCTGCCAG GTTGGCATCA
    AAAGGAACCG GTGCAGGAGA GTTCCAGGGA CAGTTACTGG GGACAATTGC ATTCATGGCG
    CCTGAGGTCC TAAGAGGTCA GCAGTATGGT AGGAGCTGTG ATGTATGGAG TGTTGGCTGC
    GCCATTATAG AAATGGCTTG TGCAAAACCA CCTTGGAATG CAGAAAAACA CTCCAATCAT
    CTCGCCTTGA TATTTAAGAT TGCTAGCGCA ACTACTGCAC CGTCCATCCC GTCACACCTG
    TCCCCGGGTC TGCGCGACGT GGCGCTGCGC TGCTTAGAAC TTCAGCCTCA GGACCGGCCT
    CCGTCCAGAG AGCTGCTGAA ACATCCGGTC TTCCGTACCA CGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017171006.1
    CDS3400..7407
    Translation

    Target ORF information:

    RefSeq Version XM_017315517.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315517.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGAATA AAGAAACCCT CAAAGGACTG CACAAGATGG AGGATCGCCC GGAGGAGAGA 
    ATGATCCGGG AGAAGCTCAA GGCGACCTGT ATGCCCGCCT GGAAGCACGA GTGGTTGGAG
    AGGAGGAACA GGAGAGGCCC TGTGGTGGTG AAGCCAATCC CTATTAAAGG AGATGGATCT
    GAAATGAATA ACTTGGCAGC TGAGCCCCAA GGAGAGGGCC AGGCAGGTTC CGCTGCACCA
    GCCCCCAAGG GCCGACGAAG CCCATCTCCT GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG
    CCGGAATCCC CAGGAGTGAG ACGGAAACGA GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA
    ATCACACCAC CCCGAAGAGC CCCGTCACCG GATGGCTTCT CCCCGTACAG CCCAGAGGAG
    ACGAGCCGCC GCGTGAACAA AGTGATGAGA GCCAGGCTGT ACCTGCTGCA GCAGATAGGA
    CCCAACTCTT TCCTGATTGG AGGAGACAGT CCAGACAATA AATACCGGGT GTTTATTGGG
    CCACAGAACT GCAGCTGTGG GCGTGGAGCA TTCTGTATTC ACCTCTTGTT TGTCATGCTC
    CGGGTGTTTC AGCTAGAACC CTCTGACCCC ATGTTATGGA GAAAAACTTT AAAAAATTTC
    GAGGTTGAGA GTTTGTTCCA GAAATACCAC AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA
    TCCCGGAACA CCATCCAGAA GTTTGTGTCA CGCATGTCAA ATTGTCACAC ACTGTCATCG
    TCTAGCACAT CCACATCTAG TTCAGAAAAC AGCATCAAGG ATGAAGAGGA GCAGATGTGT
    CCCATCTGCT TGCTGGGCAT GCTGGATGAG GAGAGCCTGA CTGTGTGTGA AGATGGCTGC
    AGGAACAAGC TGCACCACCA TTGCATGTCC ATCTGGGCGG AAGAGTGTAG AAGAAATAGA
    GAGCCTTTAA TATGTCCCCT TTGTAGATCT AAGTGGAGAT CCCATGACTT CTACAGCCAT
    GAGTTATCAA GCCCCGTGGA GTCCCCCACC TCCCTGCGAG CTGTCCAGCA GCCATCCTCC
    CCACAGCAGC CCGTGGCCGG ATCACAGCGA AGGAATCAGG AGAGCAGTTT TAACCTTACT
    CATTTTGGAA CCCAGCAGAT TCCTTCCGCT TACAAAGATT TGGCCGAGCC ATGGATTCAG
    GTGTTTGGAA TGGAACTCGT TGGCTGCTTA TTCTCTAGAA ACTGGAACGT AAGGGAAATG
    GCCCTTAGGC GTCTTTCCCA CGACGTTAGT GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC
    ACTGGAAACT CTGGAGGCGG CAGTGGGGGC AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC
    TCCCAGCCCA GCATCTCAGG GGATGTGGTG GAGGCGTGCT GCAGTGTCCT GTCTATAGTC
    TGCGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACATTGAG AGCCATGCTG
    GTATACACTC CTTGCCACAG TCTGGCAGAA AGAATCAAAC TTCAGAGACT CCTCCGGCCA
    GTTGTAGACA CTATCCTTGT CAAGTGTGCA GATGCCAACA GCCGCACGAG TCAGCTGTCC
    ATATCTACAG TGCTGGAACT CTGCAAGGGC CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA
    ATACTTAAAG CTGGGTCCAT CGGGGTTGGT GGTGTCGATT ACGTCTTAAG TTGTATCCTT
    GGAAACCAAG CTGAATCAAA CAACTGGCAA GAACTGCTGG GTCGCCTCTG TCTTATAGAC
    AGGTTGCTGT TGGAATTTCC TGCTGAATTC TATCCTCATA TTGTCAGTAC TGATGTCTCA
    CAAGCTGAGC CTGTTGAAAT CAGGTACAAG AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG
    CAATCCATTG ACAATTCCCA CTCGATGGTT GGCAAGCTCT CTCGGAGGAT ATATCTGAGC
    TCTGCCAGGA TGGTGACCGC AGTGCCCGCT GTGTTTTCCA AGCTGGTAAC CATGCTTAAT
    GCTTCTGGCT CCACCCACTT CACCAGGATG CGCCGGCGTC TGATGGCTAT CGCGGATGAG
    GTAGAAATTG CCGAGGTCAT CCAGCTGGGT GTGGAGGACA CTGTGGATGG GCATCAGGAC
    AGCTTACAGG TGCTGGCCCC CGCCAGCTGT CTAGAAAACA GCTCCCTTGA GCACACAGTC
    CATAGAGAGA AAACTGGAAA AGGACTAAGT GCTACGAGAC TGAGTGCCAG CTCGGAGGAC
    ATTTCTGACA GACTGGCCGG CGTCTCTGTA GGACTTCCCA GCTCAACAAC AACAGAACAA
    CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA CCCCACAGTC AGTGTTTGAA CTCCTCCCCT
    TTGTCTCATG CTCAATTAAT GTTCCCAGCA CCATCAGCCC CTTGTTCCTC TGCCCCGTCT
    GTCCCAGATA TTTCTAAGCA CAGACCCCAG GCATTTGTTC CCTGCAAAAT ACCTTCCGCA
    TCTCCTCAGA CACAGCGCAA GTTCTCTCTA CAATTCCAGA GGAACTGCTC TGAACACCGA
    GACTCAGACC AGCTCTCCCC AGTCTTCACT CAGTCAAGGC CCCCACCCTC CAGTAACATA
    CACAGGCCAA AGCCATCCCG ACCCGTTCCG GGCAGTACAA GCAAACTAGG GGACGCCACA
    AAAAGTAGCA TGACACTTGA TCTGGGCAGT GCTTCCAGGT GTGACGACAG CTTTGGCGGC
    GGCGGCAACA GTGGCAACGC CGTCATACCC AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG
    GACAAGTGCA GGTTAGATGT GAACACCGAG CTCAACTCCA GCATCGAGGA CCTTCTTGAA
    GCATCCATGC CTTCAAGTGA CACGACAGTC ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT
    CCGGAAAAGG CCGAAAATGA CGACACCTAC AAAGACGACG TCAATCATAA TCAAAAGTGC
    AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG GCTTTAGCGA TCGCCATGGC GATGTCAGCG
    TCTCAGGATG CCCTCCCCAT CGTCCCTCAG CTGCAGGTGG AAAATGGAGA AGATATTATC
    ATCATTCAGC AGGACACACC AGAAACTCTT CCAGGACATA CCAAAGCGAA ACAGCCTTAC
    AGAGAAGACG CTGAGTGGCT GAAAGGCCAG CAGATAGGCC TCGGAGCATT TTCTTCCTGT
    TACCAAGCAC AGGATGTGGG GACTGGGACT TTAATGGCTG TGAAACAGGT GACGTACGTC
    AGAAACACAT CCTCCGAGCA GGAGGAGGTG GTGGAAGCGT TGAGGGAAGA GATCCGGATG
    ATGGGTCACC TCAACCATCC AAACATCATC CGGATGCTGG GGGCCACGTG CGAGAAGAGC
    AACTACAACC TCTTCATTGA GTGGATGGCG GGAGGATCTG TGGCTCACCT CTTGAGTAAA
    TACGGAGCTT TCAAGGAGTC AGTCGTCATT AACTACACTG AGCAGTTACT CCGTGGCCTT
    TCCTATCTCC ACGAGAACCA GATCATTCAC AGAGACGTCA AAGGTGCCAA CCTGCTCATT
    GACAGCACCG GTCAGAGGCT GAGAATTGCA GACTTTGGAG CTGCTGCCAG GTTGGCATCA
    AAAGGAACCG GTGCAGGAGA GTTCCAGGGA CAGTTACTGG GGACAATTGC ATTCATGGCG
    CCTGAGGTCC TAAGAGGTCA GCAGTATGGT AGGAGCTGTG ATGTATGGAG TGTTGGCTGC
    GCCATTATAG AAATGGCTTG TGCAAAACCA CCTTGGAATG CAGAAAAACA CTCCAATCAT
    CTCGCCTTGA TATTTAAGAT TGCTAGCGCA ACTACTGCAC CGTCCATCCC GTCACACCTG
    TCCCCGGGTC TGCGCGACGT GGCGCTGCGC TGCTTAGAAC TTCAGCCTCA GGACCGGCCT
    CCGTCCAGAG AGCTGCTGAA ACATCCGGTC TTCCGTACCA CGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu49052
    Clone ID Related Accession (Same CDS sequence) XM_017315516.1 , XM_017315516.2
    Accession Version XM_017315516.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315516.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTCCTTC CGCCAGAAGA CAGTCGAGAG ATGGAGAATA AAGAAACCCT CAAAGGACTG 
    CACAAGATGG AGGATCGCCC GGAGGAGAGA ATGATCCGGG AGAAGCTCAA GGCGACCTGT
    ATGCCCGCCT GGAAGCACGA GTGGTTGGAG AGGAGGAACA GGAGAGGCCC TGTGGTGGTG
    AAGCCAATCC CTATTAAAGG AGATGGATCT GAAATGAATA ACTTGGCAGC TGAGCCCCAA
    GGAGAGGGCC AGGCAGGTTC CGCTGCACCA GCCCCCAAGG GCCGACGAAG CCCATCTCCT
    GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG CCGGAATCCC CAGGAGTGAG ACGGAAACGA
    GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA ATCACACCAC CCCGAAGAGC CCCGTCACCG
    GATGGCTTCT CCCCGTACAG CCCAGAGGAG ACGAGCCGCC GCGTGAACAA AGTGATGAGA
    GCCAGGCTGT ACCTGCTGCA GCAGATAGGA CCCAACTCTT TCCTGATTGG AGGAGACAGT
    CCAGACAATA AATACCGGGT GTTTATTGGG CCACAGAACT GCAGCTGTGG GCGTGGAGCA
    TTCTGTATTC ACCTCTTGTT TGTCATGCTC CGGGTGTTTC AGCTAGAACC CTCTGACCCC
    ATGTTATGGA GAAAAACTTT AAAAAATTTC GAGGTTGAGA GTTTGTTCCA GAAATACCAC
    AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA TCCCGGAACA CCATCCAGAA GTTTGTGTCA
    CGCATGTCAA ATTGTCACAC ACTGTCATCG TCTAGCACAT CCACATCTAG TTCAGAAAAC
    AGCATCAAGG ATGAAGAGGA GCAGATGTGT CCCATCTGCT TGCTGGGCAT GCTGGATGAG
    GAGAGCCTGA CTGTGTGTGA AGATGGCTGC AGGAACAAGC TGCACCACCA TTGCATGTCC
    ATCTGGGCGG AAGAGTGTAG AAGAAATAGA GAGCCTTTAA TATGTCCCCT TTGTAGATCT
    AAGTGGAGAT CCCATGACTT CTACAGCCAT GAGTTATCAA GCCCCGTGGA GTCCCCCACC
    TCCCTGCGAG CTGTCCAGCA GCCATCCTCC CCACAGCAGC CCGTGGCCGG ATCACAGCGA
    AGGAATCAGG AGAGCAGTTT TAACCTTACT CATTTTGGAA CCCAGCAGAT TCCTTCCGCT
    TACAAAGATT TGGCCGAGCC ATGGATTCAG GTGTTTGGAA TGGAACTCGT TGGCTGCTTA
    TTCTCTAGAA ACTGGAACGT AAGGGAAATG GCCCTTAGGC GTCTTTCCCA CGACGTTAGT
    GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC ACTGGAAACT CTGGAGGCGG CAGTGGGGGC
    AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC TCCCAGCCCA GCATCTCAGG GGATGTGGTG
    GAGGCGTGCT GCAGTGTCCT GTCTATAGTC TGCGCTGACC CTGTCTACAA AGTGTACGTT
    GCTGCTTTAA AAACATTGAG AGCCATGCTG GTATACACTC CTTGCCACAG TCTGGCAGAA
    AGAATCAAAC TTCAGAGACT CCTCCGGCCA GTTGTAGACA CTATCCTTGT CAAGTGTGCA
    GATGCCAACA GCCGCACGAG TCAGCTGTCC ATATCTACAG TGCTGGAACT CTGCAAGGGC
    CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA ATACTTAAAG CTGGGTCCAT CGGGGTTGGT
    GGTGTCGATT ACGTCTTAAG TTGTATCCTT GGAAACCAAG CTGAATCAAA CAACTGGCAA
    GAACTGCTGG GTCGCCTCTG TCTTATAGAC AGGTTGCTGT TGGAATTTCC TGCTGAATTC
    TATCCTCATA TTGTCAGTAC TGATGTCTCA CAAGCTGAGC CTGTTGAAAT CAGGTACAAG
    AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG CAATCCATTG ACAATTCCCA CTCGATGGTT
    GGCAAGCTCT CTCGGAGGAT ATATCTGAGC TCTGCCAGGA TGGTGACCGC AGTGCCCGCT
    GTGTTTTCCA AGCTGGTAAC CATGCTTAAT GCTTCTGGCT CCACCCACTT CACCAGGATG
    CGCCGGCGTC TGATGGCTAT CGCGGATGAG GTAGAAATTG CCGAGGTCAT CCAGCTGGGT
    GTGGAGGACA CTGTGGATGG GCATCAGGAC AGCTTACAGG TGCTGGCCCC CGCCAGCTGT
    CTAGAAAACA GCTCCCTTGA GCACACAGTC CATAGAGAGA AAACTGGAAA AGGACTAAGT
    GCTACGAGAC TGAGTGCCAG CTCGGAGGAC ATTTCTGACA GACTGGCCGG CGTCTCTGTA
    GGACTTCCCA GCTCAACAAC AACAGAACAA CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA
    CCCCACAGTC AGTGTTTGAA CTCCTCCCCT TTGTCTCATG CTCAATTAAT GTTCCCAGCA
    CCATCAGCCC CTTGTTCCTC TGCCCCGTCT GTCCCAGATA TTTCTAAGCA CAGACCCCAG
    GCATTTGTTC CCTGCAAAAT ACCTTCCGCA TCTCCTCAGA CACAGCGCAA GTTCTCTCTA
    CAATTCCAGA GGAACTGCTC TGAACACCGA GACTCAGACC AGCTCTCCCC AGTCTTCACT
    CAGTCAAGGC CCCCACCCTC CAGTAACATA CACAGGCCAA AGCCATCCCG ACCCGTTCCG
    GGCAGTACAA GCAAACTAGG GGACGCCACA AAAAGTAGCA TGACACTTGA TCTGGGCAGT
    GCTTCCAGGT GTGACGACAG CTTTGGCGGC GGCGGCAACA GTGGCAACGC CGTCATACCC
    AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG GACAAGTGCA GGTTAGATGT GAACACCGAG
    CTCAACTCCA GCATCGAGGA CCTTCTTGAA GCATCCATGC CTTCAAGTGA CACGACAGTC
    ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT CCGGAAAAGG CCGAAAATGA CGACACCTAC
    AAAGACGACG TCAATCATAA TCAAAAGTGC AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG
    GCTTTAGCGA TCGCCATGGC GATGTCAGCG TCTCAGGATG CCCTCCCCAT CGTCCCTCAG
    CTGCAGGTGG AAAATGGAGA AGATATTATC ATCATTCAGC AGGACACACC AGAAACTCTT
    CCAGGACATA CCAAAGCGAA ACAGCCTTAC AGAGAAGACG CTGAGTGGCT GAAAGGCCAG
    CAGATAGGCC TCGGAGCATT TTCTTCCTGT TACCAAGCAC AGGATGTGGG GACTGGGACT
    TTAATGGCTG TGAAACAGGT GACGTACGTC AGAAACACAT CCTCCGAGCA GGAGGAGGTG
    GTGGAAGCGT TGAGGGAAGA GATCCGGATG ATGGGTCACC TCAACCATCC AAACATCATC
    CGGATGCTGG GGGCCACGTG CGAGAAGAGC AACTACAACC TCTTCATTGA GTGGATGGCG
    GGAGGATCTG TGGCTCACCT CTTGAGTAAA TACGGAGCTT TCAAGGAGTC AGTCGTCATT
    AACTACACTG AGCAGTTACT CCGTGGCCTT TCCTATCTCC ACGAGAACCA GATCATTCAC
    AGAGACGTCA AAGGTGCCAA CCTGCTCATT GACAGCACCG GTCAGAGGCT GAGAATTGCA
    GACTTTGGAG CTGCTGCCAG GTTGGCATCA AAAGGAACCG GTGCAGGAGA GTTCCAGGGA
    CAGTTACTGG GGACAATTGC ATTCATGGCG CCTGAGGTCC TAAGAGGTCA GCAGTATGGT
    AGGAGCTGTG ATGTATGGAG TGTTGGCTGC GCCATTATAG AAATGGCTTG TGCAAAACCA
    CCTTGGAATG CAGAAAAACA CTCCAATCAT CTCGCCTTGA TATTTAAGAT TGCTAGCGCA
    ACTACTGCAC CGTCCATCCC GTCACACCTG TCCCCGGGTC TGCGCGACGT GGCGCTGCGC
    TGCTTAGAAC TTCAGCCTCA GGACCGGCCT CCGTCCAGAG AGCTGCTGAA ACATCCGGTC
    TTCCGTACCA CGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017171005.1
    CDS16..4053
    Translation

    Target ORF information:

    RefSeq Version XM_017315516.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315516.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTCCTTC CGCCAGAAGA CAGTCGAGAG ATGGAGAATA AAGAAACCCT CAAAGGACTG 
    CACAAGATGG AGGATCGCCC GGAGGAGAGA ATGATCCGGG AGAAGCTCAA GGCGACCTGT
    ATGCCCGCCT GGAAGCACGA GTGGTTGGAG AGGAGGAACA GGAGAGGCCC TGTGGTGGTG
    AAGCCAATCC CTATTAAAGG AGATGGATCT GAAATGAATA ACTTGGCAGC TGAGCCCCAA
    GGAGAGGGCC AGGCAGGTTC CGCTGCACCA GCCCCCAAGG GCCGACGAAG CCCATCTCCT
    GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG CCGGAATCCC CAGGAGTGAG ACGGAAACGA
    GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA ATCACACCAC CCCGAAGAGC CCCGTCACCG
    GATGGCTTCT CCCCGTACAG CCCAGAGGAG ACGAGCCGCC GCGTGAACAA AGTGATGAGA
    GCCAGGCTGT ACCTGCTGCA GCAGATAGGA CCCAACTCTT TCCTGATTGG AGGAGACAGT
    CCAGACAATA AATACCGGGT GTTTATTGGG CCACAGAACT GCAGCTGTGG GCGTGGAGCA
    TTCTGTATTC ACCTCTTGTT TGTCATGCTC CGGGTGTTTC AGCTAGAACC CTCTGACCCC
    ATGTTATGGA GAAAAACTTT AAAAAATTTC GAGGTTGAGA GTTTGTTCCA GAAATACCAC
    AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA TCCCGGAACA CCATCCAGAA GTTTGTGTCA
    CGCATGTCAA ATTGTCACAC ACTGTCATCG TCTAGCACAT CCACATCTAG TTCAGAAAAC
    AGCATCAAGG ATGAAGAGGA GCAGATGTGT CCCATCTGCT TGCTGGGCAT GCTGGATGAG
    GAGAGCCTGA CTGTGTGTGA AGATGGCTGC AGGAACAAGC TGCACCACCA TTGCATGTCC
    ATCTGGGCGG AAGAGTGTAG AAGAAATAGA GAGCCTTTAA TATGTCCCCT TTGTAGATCT
    AAGTGGAGAT CCCATGACTT CTACAGCCAT GAGTTATCAA GCCCCGTGGA GTCCCCCACC
    TCCCTGCGAG CTGTCCAGCA GCCATCCTCC CCACAGCAGC CCGTGGCCGG ATCACAGCGA
    AGGAATCAGG AGAGCAGTTT TAACCTTACT CATTTTGGAA CCCAGCAGAT TCCTTCCGCT
    TACAAAGATT TGGCCGAGCC ATGGATTCAG GTGTTTGGAA TGGAACTCGT TGGCTGCTTA
    TTCTCTAGAA ACTGGAACGT AAGGGAAATG GCCCTTAGGC GTCTTTCCCA CGACGTTAGT
    GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC ACTGGAAACT CTGGAGGCGG CAGTGGGGGC
    AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC TCCCAGCCCA GCATCTCAGG GGATGTGGTG
    GAGGCGTGCT GCAGTGTCCT GTCTATAGTC TGCGCTGACC CTGTCTACAA AGTGTACGTT
    GCTGCTTTAA AAACATTGAG AGCCATGCTG GTATACACTC CTTGCCACAG TCTGGCAGAA
    AGAATCAAAC TTCAGAGACT CCTCCGGCCA GTTGTAGACA CTATCCTTGT CAAGTGTGCA
    GATGCCAACA GCCGCACGAG TCAGCTGTCC ATATCTACAG TGCTGGAACT CTGCAAGGGC
    CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA ATACTTAAAG CTGGGTCCAT CGGGGTTGGT
    GGTGTCGATT ACGTCTTAAG TTGTATCCTT GGAAACCAAG CTGAATCAAA CAACTGGCAA
    GAACTGCTGG GTCGCCTCTG TCTTATAGAC AGGTTGCTGT TGGAATTTCC TGCTGAATTC
    TATCCTCATA TTGTCAGTAC TGATGTCTCA CAAGCTGAGC CTGTTGAAAT CAGGTACAAG
    AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG CAATCCATTG ACAATTCCCA CTCGATGGTT
    GGCAAGCTCT CTCGGAGGAT ATATCTGAGC TCTGCCAGGA TGGTGACCGC AGTGCCCGCT
    GTGTTTTCCA AGCTGGTAAC CATGCTTAAT GCTTCTGGCT CCACCCACTT CACCAGGATG
    CGCCGGCGTC TGATGGCTAT CGCGGATGAG GTAGAAATTG CCGAGGTCAT CCAGCTGGGT
    GTGGAGGACA CTGTGGATGG GCATCAGGAC AGCTTACAGG TGCTGGCCCC CGCCAGCTGT
    CTAGAAAACA GCTCCCTTGA GCACACAGTC CATAGAGAGA AAACTGGAAA AGGACTAAGT
    GCTACGAGAC TGAGTGCCAG CTCGGAGGAC ATTTCTGACA GACTGGCCGG CGTCTCTGTA
    GGACTTCCCA GCTCAACAAC AACAGAACAA CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA
    CCCCACAGTC AGTGTTTGAA CTCCTCCCCT TTGTCTCATG CTCAATTAAT GTTCCCAGCA
    CCATCAGCCC CTTGTTCCTC TGCCCCGTCT GTCCCAGATA TTTCTAAGCA CAGACCCCAG
    GCATTTGTTC CCTGCAAAAT ACCTTCCGCA TCTCCTCAGA CACAGCGCAA GTTCTCTCTA
    CAATTCCAGA GGAACTGCTC TGAACACCGA GACTCAGACC AGCTCTCCCC AGTCTTCACT
    CAGTCAAGGC CCCCACCCTC CAGTAACATA CACAGGCCAA AGCCATCCCG ACCCGTTCCG
    GGCAGTACAA GCAAACTAGG GGACGCCACA AAAAGTAGCA TGACACTTGA TCTGGGCAGT
    GCTTCCAGGT GTGACGACAG CTTTGGCGGC GGCGGCAACA GTGGCAACGC CGTCATACCC
    AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG GACAAGTGCA GGTTAGATGT GAACACCGAG
    CTCAACTCCA GCATCGAGGA CCTTCTTGAA GCATCCATGC CTTCAAGTGA CACGACAGTC
    ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT CCGGAAAAGG CCGAAAATGA CGACACCTAC
    AAAGACGACG TCAATCATAA TCAAAAGTGC AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG
    GCTTTAGCGA TCGCCATGGC GATGTCAGCG TCTCAGGATG CCCTCCCCAT CGTCCCTCAG
    CTGCAGGTGG AAAATGGAGA AGATATTATC ATCATTCAGC AGGACACACC AGAAACTCTT
    CCAGGACATA CCAAAGCGAA ACAGCCTTAC AGAGAAGACG CTGAGTGGCT GAAAGGCCAG
    CAGATAGGCC TCGGAGCATT TTCTTCCTGT TACCAAGCAC AGGATGTGGG GACTGGGACT
    TTAATGGCTG TGAAACAGGT GACGTACGTC AGAAACACAT CCTCCGAGCA GGAGGAGGTG
    GTGGAAGCGT TGAGGGAAGA GATCCGGATG ATGGGTCACC TCAACCATCC AAACATCATC
    CGGATGCTGG GGGCCACGTG CGAGAAGAGC AACTACAACC TCTTCATTGA GTGGATGGCG
    GGAGGATCTG TGGCTCACCT CTTGAGTAAA TACGGAGCTT TCAAGGAGTC AGTCGTCATT
    AACTACACTG AGCAGTTACT CCGTGGCCTT TCCTATCTCC ACGAGAACCA GATCATTCAC
    AGAGACGTCA AAGGTGCCAA CCTGCTCATT GACAGCACCG GTCAGAGGCT GAGAATTGCA
    GACTTTGGAG CTGCTGCCAG GTTGGCATCA AAAGGAACCG GTGCAGGAGA GTTCCAGGGA
    CAGTTACTGG GGACAATTGC ATTCATGGCG CCTGAGGTCC TAAGAGGTCA GCAGTATGGT
    AGGAGCTGTG ATGTATGGAG TGTTGGCTGC GCCATTATAG AAATGGCTTG TGCAAAACCA
    CCTTGGAATG CAGAAAAACA CTCCAATCAT CTCGCCTTGA TATTTAAGAT TGCTAGCGCA
    ACTACTGCAC CGTCCATCCC GTCACACCTG TCCCCGGGTC TGCGCGACGT GGCGCTGCGC
    TGCTTAGAAC TTCAGCCTCA GGACCGGCCT CCGTCCAGAG AGCTGCTGAA ACATCCGGTC
    TTCCGTACCA CGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu69202
    Clone ID Related Accession (Same CDS sequence) XM_017315517.2 , XM_017315517.1
    Accession Version XM_017315517.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGAATA AAGAAACCCT CAAAGGACTG CACAAGATGG AGGATCGCCC GGAGGAGAGA 
    ATGATCCGGG AGAAGCTCAA GGCGACCTGT ATGCCCGCCT GGAAGCACGA GTGGTTGGAG
    AGGAGGAACA GGAGAGGCCC TGTGGTGGTG AAGCCAATCC CTATTAAAGG AGATGGATCT
    GAAATGAATA ACTTGGCAGC TGAGCCCCAA GGAGAGGGCC AGGCAGGTTC CGCTGCACCA
    GCCCCCAAGG GCCGACGAAG CCCATCTCCT GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG
    CCGGAATCCC CAGGAGTGAG ACGGAAACGA GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA
    ATCACACCAC CCCGAAGAGC CCCGTCACCG GATGGCTTCT CCCCGTACAG CCCAGAGGAG
    ACGAGCCGCC GCGTGAACAA AGTGATGAGA GCCAGGCTGT ACCTGCTGCA GCAGATAGGA
    CCCAACTCTT TCCTGATTGG AGGAGACAGT CCAGACAATA AATACCGGGT GTTTATTGGG
    CCACAGAACT GCAGCTGTGG GCGTGGAGCA TTCTGTATTC ACCTCTTGTT TGTCATGCTC
    CGGGTGTTTC AGCTAGAACC CTCTGACCCC ATGTTATGGA GAAAAACTTT AAAAAATTTC
    GAGGTTGAGA GTTTGTTCCA GAAATACCAC AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA
    TCCCGGAACA CCATCCAGAA GTTTGTGTCA CGCATGTCAA ATTGTCACAC ACTGTCATCG
    TCTAGCACAT CCACATCTAG TTCAGAAAAC AGCATCAAGG ATGAAGAGGA GCAGATGTGT
    CCCATCTGCT TGCTGGGCAT GCTGGATGAG GAGAGCCTGA CTGTGTGTGA AGATGGCTGC
    AGGAACAAGC TGCACCACCA TTGCATGTCC ATCTGGGCGG AAGAGTGTAG AAGAAATAGA
    GAGCCTTTAA TATGTCCCCT TTGTAGATCT AAGTGGAGAT CCCATGACTT CTACAGCCAT
    GAGTTATCAA GCCCCGTGGA GTCCCCCACC TCCCTGCGAG CTGTCCAGCA GCCATCCTCC
    CCACAGCAGC CCGTGGCCGG ATCACAGCGA AGGAATCAGG AGAGCAGTTT TAACCTTACT
    CATTTTGGAA CCCAGCAGAT TCCTTCCGCT TACAAAGATT TGGCCGAGCC ATGGATTCAG
    GTGTTTGGAA TGGAACTCGT TGGCTGCTTA TTCTCTAGAA ACTGGAACGT AAGGGAAATG
    GCCCTTAGGC GTCTTTCCCA CGACGTTAGT GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC
    ACTGGAAACT CTGGAGGCGG CAGTGGGGGC AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC
    TCCCAGCCCA GCATCTCAGG GGATGTGGTG GAGGCGTGCT GCAGTGTCCT GTCTATAGTC
    TGCGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACATTGAG AGCCATGCTG
    GTATACACTC CTTGCCACAG TCTGGCAGAA AGAATCAAAC TTCAGAGACT CCTCCGGCCA
    GTTGTAGACA CTATCCTTGT CAAGTGTGCA GATGCCAACA GCCGCACGAG TCAGCTGTCC
    ATATCTACAG TGCTGGAACT CTGCAAGGGC CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA
    ATACTTAAAG CTGGGTCCAT CGGGGTTGGT GGTGTCGATT ACGTCTTAAG TTGTATCCTT
    GGAAACCAAG CTGAATCAAA CAACTGGCAA GAACTGCTGG GTCGCCTCTG TCTTATAGAC
    AGGTTGCTGT TGGAATTTCC TGCTGAATTC TATCCTCATA TTGTCAGTAC TGATGTCTCA
    CAAGCTGAGC CTGTTGAAAT CAGGTACAAG AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG
    CAATCCATTG ACAATTCCCA CTCGATGGTT GGCAAGCTCT CTCGGAGGAT ATATCTGAGC
    TCTGCCAGGA TGGTGACCGC AGTGCCCGCT GTGTTTTCCA AGCTGGTAAC CATGCTTAAT
    GCTTCTGGCT CCACCCACTT CACCAGGATG CGCCGGCGTC TGATGGCTAT CGCGGATGAG
    GTAGAAATTG CCGAGGTCAT CCAGCTGGGT GTGGAGGACA CTGTGGATGG GCATCAGGAC
    AGCTTACAGG TGCTGGCCCC CGCCAGCTGT CTAGAAAACA GCTCCCTTGA GCACACAGTC
    CATAGAGAGA AAACTGGAAA AGGACTAAGT GCTACGAGAC TGAGTGCCAG CTCGGAGGAC
    ATTTCTGACA GACTGGCCGG CGTCTCTGTA GGACTTCCCA GCTCAACAAC AACAGAACAA
    CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA CCCCACAGTC AGTGTTTGAA CTCCTCCCCT
    TTGTCTCATG CTCAATTAAT GTTCCCAGCA CCATCAGCCC CTTGTTCCTC TGCCCCGTCT
    GTCCCAGATA TTTCTAAGCA CAGACCCCAG GCATTTGTTC CCTGCAAAAT ACCTTCCGCA
    TCTCCTCAGA CACAGCGCAA GTTCTCTCTA CAATTCCAGA GGAACTGCTC TGAACACCGA
    GACTCAGACC AGCTCTCCCC AGTCTTCACT CAGTCAAGGC CCCCACCCTC CAGTAACATA
    CACAGGCCAA AGCCATCCCG ACCCGTTCCG GGCAGTACAA GCAAACTAGG GGACGCCACA
    AAAAGTAGCA TGACACTTGA TCTGGGCAGT GCTTCCAGGT GTGACGACAG CTTTGGCGGC
    GGCGGCAACA GTGGCAACGC CGTCATACCC AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG
    GACAAGTGCA GGTTAGATGT GAACACCGAG CTCAACTCCA GCATCGAGGA CCTTCTTGAA
    GCATCCATGC CTTCAAGTGA CACGACAGTC ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT
    CCGGAAAAGG CCGAAAATGA CGACACCTAC AAAGACGACG TCAATCATAA TCAAAAGTGC
    AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG GCTTTAGCGA TCGCCATGGC GATGTCAGCG
    TCTCAGGATG CCCTCCCCAT CGTCCCTCAG CTGCAGGTGG AAAATGGAGA AGATATTATC
    ATCATTCAGC AGGACACACC AGAAACTCTT CCAGGACATA CCAAAGCGAA ACAGCCTTAC
    AGAGAAGACG CTGAGTGGCT GAAAGGCCAG CAGATAGGCC TCGGAGCATT TTCTTCCTGT
    TACCAAGCAC AGGATGTGGG GACTGGGACT TTAATGGCTG TGAAACAGGT GACGTACGTC
    AGAAACACAT CCTCCGAGCA GGAGGAGGTG GTGGAAGCGT TGAGGGAAGA GATCCGGATG
    ATGGGTCACC TCAACCATCC AAACATCATC CGGATGCTGG GGGCCACGTG CGAGAAGAGC
    AACTACAACC TCTTCATTGA GTGGATGGCG GGAGGATCTG TGGCTCACCT CTTGAGTAAA
    TACGGAGCTT TCAAGGAGTC AGTCGTCATT AACTACACTG AGCAGTTACT CCGTGGCCTT
    TCCTATCTCC ACGAGAACCA GATCATTCAC AGAGACGTCA AAGGTGCCAA CCTGCTCATT
    GACAGCACCG GTCAGAGGCT GAGAATTGCA GACTTTGGAG CTGCTGCCAG GTTGGCATCA
    AAAGGAACCG GTGCAGGAGA GTTCCAGGGA CAGTTACTGG GGACAATTGC ATTCATGGCG
    CCTGAGGTCC TAAGAGGTCA GCAGTATGGT AGGAGCTGTG ATGTATGGAG TGTTGGCTGC
    GCCATTATAG AAATGGCTTG TGCAAAACCA CCTTGGAATG CAGAAAAACA CTCCAATCAT
    CTCGCCTTGA TATTTAAGAT TGCTAGCGCA ACTACTGCAC CGTCCATCCC GTCACACCTG
    TCCCCGGGTC TGCGCGACGT GGCGCTGCGC TGCTTAGAAC TTCAGCCTCA GGACCGGCCT
    CCGTCCAGAG AGCTGCTGAA ACATCCGGTC TTCCGTACCA CGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017171006.1
    CDS11916..15923
    Translation

    Target ORF information:

    RefSeq Version XM_017315517.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315517.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGAATA AAGAAACCCT CAAAGGACTG CACAAGATGG AGGATCGCCC GGAGGAGAGA 
    ATGATCCGGG AGAAGCTCAA GGCGACCTGT ATGCCCGCCT GGAAGCACGA GTGGTTGGAG
    AGGAGGAACA GGAGAGGCCC TGTGGTGGTG AAGCCAATCC CTATTAAAGG AGATGGATCT
    GAAATGAATA ACTTGGCAGC TGAGCCCCAA GGAGAGGGCC AGGCAGGTTC CGCTGCACCA
    GCCCCCAAGG GCCGACGAAG CCCATCTCCT GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG
    CCGGAATCCC CAGGAGTGAG ACGGAAACGA GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA
    ATCACACCAC CCCGAAGAGC CCCGTCACCG GATGGCTTCT CCCCGTACAG CCCAGAGGAG
    ACGAGCCGCC GCGTGAACAA AGTGATGAGA GCCAGGCTGT ACCTGCTGCA GCAGATAGGA
    CCCAACTCTT TCCTGATTGG AGGAGACAGT CCAGACAATA AATACCGGGT GTTTATTGGG
    CCACAGAACT GCAGCTGTGG GCGTGGAGCA TTCTGTATTC ACCTCTTGTT TGTCATGCTC
    CGGGTGTTTC AGCTAGAACC CTCTGACCCC ATGTTATGGA GAAAAACTTT AAAAAATTTC
    GAGGTTGAGA GTTTGTTCCA GAAATACCAC AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA
    TCCCGGAACA CCATCCAGAA GTTTGTGTCA CGCATGTCAA ATTGTCACAC ACTGTCATCG
    TCTAGCACAT CCACATCTAG TTCAGAAAAC AGCATCAAGG ATGAAGAGGA GCAGATGTGT
    CCCATCTGCT TGCTGGGCAT GCTGGATGAG GAGAGCCTGA CTGTGTGTGA AGATGGCTGC
    AGGAACAAGC TGCACCACCA TTGCATGTCC ATCTGGGCGG AAGAGTGTAG AAGAAATAGA
    GAGCCTTTAA TATGTCCCCT TTGTAGATCT AAGTGGAGAT CCCATGACTT CTACAGCCAT
    GAGTTATCAA GCCCCGTGGA GTCCCCCACC TCCCTGCGAG CTGTCCAGCA GCCATCCTCC
    CCACAGCAGC CCGTGGCCGG ATCACAGCGA AGGAATCAGG AGAGCAGTTT TAACCTTACT
    CATTTTGGAA CCCAGCAGAT TCCTTCCGCT TACAAAGATT TGGCCGAGCC ATGGATTCAG
    GTGTTTGGAA TGGAACTCGT TGGCTGCTTA TTCTCTAGAA ACTGGAACGT AAGGGAAATG
    GCCCTTAGGC GTCTTTCCCA CGACGTTAGT GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC
    ACTGGAAACT CTGGAGGCGG CAGTGGGGGC AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC
    TCCCAGCCCA GCATCTCAGG GGATGTGGTG GAGGCGTGCT GCAGTGTCCT GTCTATAGTC
    TGCGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACATTGAG AGCCATGCTG
    GTATACACTC CTTGCCACAG TCTGGCAGAA AGAATCAAAC TTCAGAGACT CCTCCGGCCA
    GTTGTAGACA CTATCCTTGT CAAGTGTGCA GATGCCAACA GCCGCACGAG TCAGCTGTCC
    ATATCTACAG TGCTGGAACT CTGCAAGGGC CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA
    ATACTTAAAG CTGGGTCCAT CGGGGTTGGT GGTGTCGATT ACGTCTTAAG TTGTATCCTT
    GGAAACCAAG CTGAATCAAA CAACTGGCAA GAACTGCTGG GTCGCCTCTG TCTTATAGAC
    AGGTTGCTGT TGGAATTTCC TGCTGAATTC TATCCTCATA TTGTCAGTAC TGATGTCTCA
    CAAGCTGAGC CTGTTGAAAT CAGGTACAAG AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG
    CAATCCATTG ACAATTCCCA CTCGATGGTT GGCAAGCTCT CTCGGAGGAT ATATCTGAGC
    TCTGCCAGGA TGGTGACCGC AGTGCCCGCT GTGTTTTCCA AGCTGGTAAC CATGCTTAAT
    GCTTCTGGCT CCACCCACTT CACCAGGATG CGCCGGCGTC TGATGGCTAT CGCGGATGAG
    GTAGAAATTG CCGAGGTCAT CCAGCTGGGT GTGGAGGACA CTGTGGATGG GCATCAGGAC
    AGCTTACAGG TGCTGGCCCC CGCCAGCTGT CTAGAAAACA GCTCCCTTGA GCACACAGTC
    CATAGAGAGA AAACTGGAAA AGGACTAAGT GCTACGAGAC TGAGTGCCAG CTCGGAGGAC
    ATTTCTGACA GACTGGCCGG CGTCTCTGTA GGACTTCCCA GCTCAACAAC AACAGAACAA
    CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA CCCCACAGTC AGTGTTTGAA CTCCTCCCCT
    TTGTCTCATG CTCAATTAAT GTTCCCAGCA CCATCAGCCC CTTGTTCCTC TGCCCCGTCT
    GTCCCAGATA TTTCTAAGCA CAGACCCCAG GCATTTGTTC CCTGCAAAAT ACCTTCCGCA
    TCTCCTCAGA CACAGCGCAA GTTCTCTCTA CAATTCCAGA GGAACTGCTC TGAACACCGA
    GACTCAGACC AGCTCTCCCC AGTCTTCACT CAGTCAAGGC CCCCACCCTC CAGTAACATA
    CACAGGCCAA AGCCATCCCG ACCCGTTCCG GGCAGTACAA GCAAACTAGG GGACGCCACA
    AAAAGTAGCA TGACACTTGA TCTGGGCAGT GCTTCCAGGT GTGACGACAG CTTTGGCGGC
    GGCGGCAACA GTGGCAACGC CGTCATACCC AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG
    GACAAGTGCA GGTTAGATGT GAACACCGAG CTCAACTCCA GCATCGAGGA CCTTCTTGAA
    GCATCCATGC CTTCAAGTGA CACGACAGTC ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT
    CCGGAAAAGG CCGAAAATGA CGACACCTAC AAAGACGACG TCAATCATAA TCAAAAGTGC
    AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG GCTTTAGCGA TCGCCATGGC GATGTCAGCG
    TCTCAGGATG CCCTCCCCAT CGTCCCTCAG CTGCAGGTGG AAAATGGAGA AGATATTATC
    ATCATTCAGC AGGACACACC AGAAACTCTT CCAGGACATA CCAAAGCGAA ACAGCCTTAC
    AGAGAAGACG CTGAGTGGCT GAAAGGCCAG CAGATAGGCC TCGGAGCATT TTCTTCCTGT
    TACCAAGCAC AGGATGTGGG GACTGGGACT TTAATGGCTG TGAAACAGGT GACGTACGTC
    AGAAACACAT CCTCCGAGCA GGAGGAGGTG GTGGAAGCGT TGAGGGAAGA GATCCGGATG
    ATGGGTCACC TCAACCATCC AAACATCATC CGGATGCTGG GGGCCACGTG CGAGAAGAGC
    AACTACAACC TCTTCATTGA GTGGATGGCG GGAGGATCTG TGGCTCACCT CTTGAGTAAA
    TACGGAGCTT TCAAGGAGTC AGTCGTCATT AACTACACTG AGCAGTTACT CCGTGGCCTT
    TCCTATCTCC ACGAGAACCA GATCATTCAC AGAGACGTCA AAGGTGCCAA CCTGCTCATT
    GACAGCACCG GTCAGAGGCT GAGAATTGCA GACTTTGGAG CTGCTGCCAG GTTGGCATCA
    AAAGGAACCG GTGCAGGAGA GTTCCAGGGA CAGTTACTGG GGACAATTGC ATTCATGGCG
    CCTGAGGTCC TAAGAGGTCA GCAGTATGGT AGGAGCTGTG ATGTATGGAG TGTTGGCTGC
    GCCATTATAG AAATGGCTTG TGCAAAACCA CCTTGGAATG CAGAAAAACA CTCCAATCAT
    CTCGCCTTGA TATTTAAGAT TGCTAGCGCA ACTACTGCAC CGTCCATCCC GTCACACCTG
    TCCCCGGGTC TGCGCGACGT GGCGCTGCGC TGCTTAGAAC TTCAGCCTCA GGACCGGCCT
    CCGTCCAGAG AGCTGCTGAA ACATCCGGTC TTCCGTACCA CGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu49052
    Clone ID Related Accession (Same CDS sequence) XM_017315516.1 , XM_017315516.2
    Accession Version XM_017315516.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTCCTTC CGCCAGAAGA CAGTCGAGAG ATGGAGAATA AAGAAACCCT CAAAGGACTG 
    CACAAGATGG AGGATCGCCC GGAGGAGAGA ATGATCCGGG AGAAGCTCAA GGCGACCTGT
    ATGCCCGCCT GGAAGCACGA GTGGTTGGAG AGGAGGAACA GGAGAGGCCC TGTGGTGGTG
    AAGCCAATCC CTATTAAAGG AGATGGATCT GAAATGAATA ACTTGGCAGC TGAGCCCCAA
    GGAGAGGGCC AGGCAGGTTC CGCTGCACCA GCCCCCAAGG GCCGACGAAG CCCATCTCCT
    GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG CCGGAATCCC CAGGAGTGAG ACGGAAACGA
    GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA ATCACACCAC CCCGAAGAGC CCCGTCACCG
    GATGGCTTCT CCCCGTACAG CCCAGAGGAG ACGAGCCGCC GCGTGAACAA AGTGATGAGA
    GCCAGGCTGT ACCTGCTGCA GCAGATAGGA CCCAACTCTT TCCTGATTGG AGGAGACAGT
    CCAGACAATA AATACCGGGT GTTTATTGGG CCACAGAACT GCAGCTGTGG GCGTGGAGCA
    TTCTGTATTC ACCTCTTGTT TGTCATGCTC CGGGTGTTTC AGCTAGAACC CTCTGACCCC
    ATGTTATGGA GAAAAACTTT AAAAAATTTC GAGGTTGAGA GTTTGTTCCA GAAATACCAC
    AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA TCCCGGAACA CCATCCAGAA GTTTGTGTCA
    CGCATGTCAA ATTGTCACAC ACTGTCATCG TCTAGCACAT CCACATCTAG TTCAGAAAAC
    AGCATCAAGG ATGAAGAGGA GCAGATGTGT CCCATCTGCT TGCTGGGCAT GCTGGATGAG
    GAGAGCCTGA CTGTGTGTGA AGATGGCTGC AGGAACAAGC TGCACCACCA TTGCATGTCC
    ATCTGGGCGG AAGAGTGTAG AAGAAATAGA GAGCCTTTAA TATGTCCCCT TTGTAGATCT
    AAGTGGAGAT CCCATGACTT CTACAGCCAT GAGTTATCAA GCCCCGTGGA GTCCCCCACC
    TCCCTGCGAG CTGTCCAGCA GCCATCCTCC CCACAGCAGC CCGTGGCCGG ATCACAGCGA
    AGGAATCAGG AGAGCAGTTT TAACCTTACT CATTTTGGAA CCCAGCAGAT TCCTTCCGCT
    TACAAAGATT TGGCCGAGCC ATGGATTCAG GTGTTTGGAA TGGAACTCGT TGGCTGCTTA
    TTCTCTAGAA ACTGGAACGT AAGGGAAATG GCCCTTAGGC GTCTTTCCCA CGACGTTAGT
    GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC ACTGGAAACT CTGGAGGCGG CAGTGGGGGC
    AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC TCCCAGCCCA GCATCTCAGG GGATGTGGTG
    GAGGCGTGCT GCAGTGTCCT GTCTATAGTC TGCGCTGACC CTGTCTACAA AGTGTACGTT
    GCTGCTTTAA AAACATTGAG AGCCATGCTG GTATACACTC CTTGCCACAG TCTGGCAGAA
    AGAATCAAAC TTCAGAGACT CCTCCGGCCA GTTGTAGACA CTATCCTTGT CAAGTGTGCA
    GATGCCAACA GCCGCACGAG TCAGCTGTCC ATATCTACAG TGCTGGAACT CTGCAAGGGC
    CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA ATACTTAAAG CTGGGTCCAT CGGGGTTGGT
    GGTGTCGATT ACGTCTTAAG TTGTATCCTT GGAAACCAAG CTGAATCAAA CAACTGGCAA
    GAACTGCTGG GTCGCCTCTG TCTTATAGAC AGGTTGCTGT TGGAATTTCC TGCTGAATTC
    TATCCTCATA TTGTCAGTAC TGATGTCTCA CAAGCTGAGC CTGTTGAAAT CAGGTACAAG
    AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG CAATCCATTG ACAATTCCCA CTCGATGGTT
    GGCAAGCTCT CTCGGAGGAT ATATCTGAGC TCTGCCAGGA TGGTGACCGC AGTGCCCGCT
    GTGTTTTCCA AGCTGGTAAC CATGCTTAAT GCTTCTGGCT CCACCCACTT CACCAGGATG
    CGCCGGCGTC TGATGGCTAT CGCGGATGAG GTAGAAATTG CCGAGGTCAT CCAGCTGGGT
    GTGGAGGACA CTGTGGATGG GCATCAGGAC AGCTTACAGG TGCTGGCCCC CGCCAGCTGT
    CTAGAAAACA GCTCCCTTGA GCACACAGTC CATAGAGAGA AAACTGGAAA AGGACTAAGT
    GCTACGAGAC TGAGTGCCAG CTCGGAGGAC ATTTCTGACA GACTGGCCGG CGTCTCTGTA
    GGACTTCCCA GCTCAACAAC AACAGAACAA CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA
    CCCCACAGTC AGTGTTTGAA CTCCTCCCCT TTGTCTCATG CTCAATTAAT GTTCCCAGCA
    CCATCAGCCC CTTGTTCCTC TGCCCCGTCT GTCCCAGATA TTTCTAAGCA CAGACCCCAG
    GCATTTGTTC CCTGCAAAAT ACCTTCCGCA TCTCCTCAGA CACAGCGCAA GTTCTCTCTA
    CAATTCCAGA GGAACTGCTC TGAACACCGA GACTCAGACC AGCTCTCCCC AGTCTTCACT
    CAGTCAAGGC CCCCACCCTC CAGTAACATA CACAGGCCAA AGCCATCCCG ACCCGTTCCG
    GGCAGTACAA GCAAACTAGG GGACGCCACA AAAAGTAGCA TGACACTTGA TCTGGGCAGT
    GCTTCCAGGT GTGACGACAG CTTTGGCGGC GGCGGCAACA GTGGCAACGC CGTCATACCC
    AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG GACAAGTGCA GGTTAGATGT GAACACCGAG
    CTCAACTCCA GCATCGAGGA CCTTCTTGAA GCATCCATGC CTTCAAGTGA CACGACAGTC
    ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT CCGGAAAAGG CCGAAAATGA CGACACCTAC
    AAAGACGACG TCAATCATAA TCAAAAGTGC AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG
    GCTTTAGCGA TCGCCATGGC GATGTCAGCG TCTCAGGATG CCCTCCCCAT CGTCCCTCAG
    CTGCAGGTGG AAAATGGAGA AGATATTATC ATCATTCAGC AGGACACACC AGAAACTCTT
    CCAGGACATA CCAAAGCGAA ACAGCCTTAC AGAGAAGACG CTGAGTGGCT GAAAGGCCAG
    CAGATAGGCC TCGGAGCATT TTCTTCCTGT TACCAAGCAC AGGATGTGGG GACTGGGACT
    TTAATGGCTG TGAAACAGGT GACGTACGTC AGAAACACAT CCTCCGAGCA GGAGGAGGTG
    GTGGAAGCGT TGAGGGAAGA GATCCGGATG ATGGGTCACC TCAACCATCC AAACATCATC
    CGGATGCTGG GGGCCACGTG CGAGAAGAGC AACTACAACC TCTTCATTGA GTGGATGGCG
    GGAGGATCTG TGGCTCACCT CTTGAGTAAA TACGGAGCTT TCAAGGAGTC AGTCGTCATT
    AACTACACTG AGCAGTTACT CCGTGGCCTT TCCTATCTCC ACGAGAACCA GATCATTCAC
    AGAGACGTCA AAGGTGCCAA CCTGCTCATT GACAGCACCG GTCAGAGGCT GAGAATTGCA
    GACTTTGGAG CTGCTGCCAG GTTGGCATCA AAAGGAACCG GTGCAGGAGA GTTCCAGGGA
    CAGTTACTGG GGACAATTGC ATTCATGGCG CCTGAGGTCC TAAGAGGTCA GCAGTATGGT
    AGGAGCTGTG ATGTATGGAG TGTTGGCTGC GCCATTATAG AAATGGCTTG TGCAAAACCA
    CCTTGGAATG CAGAAAAACA CTCCAATCAT CTCGCCTTGA TATTTAAGAT TGCTAGCGCA
    ACTACTGCAC CGTCCATCCC GTCACACCTG TCCCCGGGTC TGCGCGACGT GGCGCTGCGC
    TGCTTAGAAC TTCAGCCTCA GGACCGGCCT CCGTCCAGAG AGCTGCTGAA ACATCCGGTC
    TTCCGTACCA CGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017171005.1
    CDS16..4053
    Translation

    Target ORF information:

    RefSeq Version XM_017315516.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315516.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTCCTTC CGCCAGAAGA CAGTCGAGAG ATGGAGAATA AAGAAACCCT CAAAGGACTG 
    CACAAGATGG AGGATCGCCC GGAGGAGAGA ATGATCCGGG AGAAGCTCAA GGCGACCTGT
    ATGCCCGCCT GGAAGCACGA GTGGTTGGAG AGGAGGAACA GGAGAGGCCC TGTGGTGGTG
    AAGCCAATCC CTATTAAAGG AGATGGATCT GAAATGAATA ACTTGGCAGC TGAGCCCCAA
    GGAGAGGGCC AGGCAGGTTC CGCTGCACCA GCCCCCAAGG GCCGACGAAG CCCATCTCCT
    GGCAGCTCTC CGTCAGGGCG CTCGGTGAAG CCGGAATCCC CAGGAGTGAG ACGGAAACGA
    GTGTCCCCGG TGCCTTTCCA GAGTGGCAGA ATCACACCAC CCCGAAGAGC CCCGTCACCG
    GATGGCTTCT CCCCGTACAG CCCAGAGGAG ACGAGCCGCC GCGTGAACAA AGTGATGAGA
    GCCAGGCTGT ACCTGCTGCA GCAGATAGGA CCCAACTCTT TCCTGATTGG AGGAGACAGT
    CCAGACAATA AATACCGGGT GTTTATTGGG CCACAGAACT GCAGCTGTGG GCGTGGAGCA
    TTCTGTATTC ACCTCTTGTT TGTCATGCTC CGGGTGTTTC AGCTAGAACC CTCTGACCCC
    ATGTTATGGA GAAAAACTTT AAAAAATTTC GAGGTTGAGA GTTTGTTCCA GAAATACCAC
    AGTAGGCGTA GCTCGAGAAT CAAAGCTCCA TCCCGGAACA CCATCCAGAA GTTTGTGTCA
    CGCATGTCAA ATTGTCACAC ACTGTCATCG TCTAGCACAT CCACATCTAG TTCAGAAAAC
    AGCATCAAGG ATGAAGAGGA GCAGATGTGT CCCATCTGCT TGCTGGGCAT GCTGGATGAG
    GAGAGCCTGA CTGTGTGTGA AGATGGCTGC AGGAACAAGC TGCACCACCA TTGCATGTCC
    ATCTGGGCGG AAGAGTGTAG AAGAAATAGA GAGCCTTTAA TATGTCCCCT TTGTAGATCT
    AAGTGGAGAT CCCATGACTT CTACAGCCAT GAGTTATCAA GCCCCGTGGA GTCCCCCACC
    TCCCTGCGAG CTGTCCAGCA GCCATCCTCC CCACAGCAGC CCGTGGCCGG ATCACAGCGA
    AGGAATCAGG AGAGCAGTTT TAACCTTACT CATTTTGGAA CCCAGCAGAT TCCTTCCGCT
    TACAAAGATT TGGCCGAGCC ATGGATTCAG GTGTTTGGAA TGGAACTCGT TGGCTGCTTA
    TTCTCTAGAA ACTGGAACGT AAGGGAAATG GCCCTTAGGC GTCTTTCCCA CGACGTTAGT
    GGGGCCCTGT TGTTGGCAAA CGGGGAGAGC ACTGGAAACT CTGGAGGCGG CAGTGGGGGC
    AGCTTAAGCG CGGGAGCGGC CAGCGGGTCC TCCCAGCCCA GCATCTCAGG GGATGTGGTG
    GAGGCGTGCT GCAGTGTCCT GTCTATAGTC TGCGCTGACC CTGTCTACAA AGTGTACGTT
    GCTGCTTTAA AAACATTGAG AGCCATGCTG GTATACACTC CTTGCCACAG TCTGGCAGAA
    AGAATCAAAC TTCAGAGACT CCTCCGGCCA GTTGTAGACA CTATCCTTGT CAAGTGTGCA
    GATGCCAACA GCCGCACGAG TCAGCTGTCC ATATCTACAG TGCTGGAACT CTGCAAGGGC
    CAAGCAGGAG AGCTGGCGGT TGGGAGAGAA ATACTTAAAG CTGGGTCCAT CGGGGTTGGT
    GGTGTCGATT ACGTCTTAAG TTGTATCCTT GGAAACCAAG CTGAATCAAA CAACTGGCAA
    GAACTGCTGG GTCGCCTCTG TCTTATAGAC AGGTTGCTGT TGGAATTTCC TGCTGAATTC
    TATCCTCATA TTGTCAGTAC TGATGTCTCA CAAGCTGAGC CTGTTGAAAT CAGGTACAAG
    AAGCTGCTCT CCCTCTTAAC CTTTGCCTTG CAATCCATTG ACAATTCCCA CTCGATGGTT
    GGCAAGCTCT CTCGGAGGAT ATATCTGAGC TCTGCCAGGA TGGTGACCGC AGTGCCCGCT
    GTGTTTTCCA AGCTGGTAAC CATGCTTAAT GCTTCTGGCT CCACCCACTT CACCAGGATG
    CGCCGGCGTC TGATGGCTAT CGCGGATGAG GTAGAAATTG CCGAGGTCAT CCAGCTGGGT
    GTGGAGGACA CTGTGGATGG GCATCAGGAC AGCTTACAGG TGCTGGCCCC CGCCAGCTGT
    CTAGAAAACA GCTCCCTTGA GCACACAGTC CATAGAGAGA AAACTGGAAA AGGACTAAGT
    GCTACGAGAC TGAGTGCCAG CTCGGAGGAC ATTTCTGACA GACTGGCCGG CGTCTCTGTA
    GGACTTCCCA GCTCAACAAC AACAGAACAA CCAAAGCCAG CGGTTCAAAC AAAAGGCAGA
    CCCCACAGTC AGTGTTTGAA CTCCTCCCCT TTGTCTCATG CTCAATTAAT GTTCCCAGCA
    CCATCAGCCC CTTGTTCCTC TGCCCCGTCT GTCCCAGATA TTTCTAAGCA CAGACCCCAG
    GCATTTGTTC CCTGCAAAAT ACCTTCCGCA TCTCCTCAGA CACAGCGCAA GTTCTCTCTA
    CAATTCCAGA GGAACTGCTC TGAACACCGA GACTCAGACC AGCTCTCCCC AGTCTTCACT
    CAGTCAAGGC CCCCACCCTC CAGTAACATA CACAGGCCAA AGCCATCCCG ACCCGTTCCG
    GGCAGTACAA GCAAACTAGG GGACGCCACA AAAAGTAGCA TGACACTTGA TCTGGGCAGT
    GCTTCCAGGT GTGACGACAG CTTTGGCGGC GGCGGCAACA GTGGCAACGC CGTCATACCC
    AGCGAAGAGA CAGTGTTCAC GCCGGTGGAG GACAAGTGCA GGTTAGATGT GAACACCGAG
    CTCAACTCCA GCATCGAGGA CCTTCTTGAA GCATCCATGC CTTCAAGTGA CACGACAGTC
    ACTTTCAAGT CCGAAGTCGC CGTCCTCTCT CCGGAAAAGG CCGAAAATGA CGACACCTAC
    AAAGACGACG TCAATCATAA TCAAAAGTGC AAAGAAAAGA TGGAAGCTGA AGAGGAGGAG
    GCTTTAGCGA TCGCCATGGC GATGTCAGCG TCTCAGGATG CCCTCCCCAT CGTCCCTCAG
    CTGCAGGTGG AAAATGGAGA AGATATTATC ATCATTCAGC AGGACACACC AGAAACTCTT
    CCAGGACATA CCAAAGCGAA ACAGCCTTAC AGAGAAGACG CTGAGTGGCT GAAAGGCCAG
    CAGATAGGCC TCGGAGCATT TTCTTCCTGT TACCAAGCAC AGGATGTGGG GACTGGGACT
    TTAATGGCTG TGAAACAGGT GACGTACGTC AGAAACACAT CCTCCGAGCA GGAGGAGGTG
    GTGGAAGCGT TGAGGGAAGA GATCCGGATG ATGGGTCACC TCAACCATCC AAACATCATC
    CGGATGCTGG GGGCCACGTG CGAGAAGAGC AACTACAACC TCTTCATTGA GTGGATGGCG
    GGAGGATCTG TGGCTCACCT CTTGAGTAAA TACGGAGCTT TCAAGGAGTC AGTCGTCATT
    AACTACACTG AGCAGTTACT CCGTGGCCTT TCCTATCTCC ACGAGAACCA GATCATTCAC
    AGAGACGTCA AAGGTGCCAA CCTGCTCATT GACAGCACCG GTCAGAGGCT GAGAATTGCA
    GACTTTGGAG CTGCTGCCAG GTTGGCATCA AAAGGAACCG GTGCAGGAGA GTTCCAGGGA
    CAGTTACTGG GGACAATTGC ATTCATGGCG CCTGAGGTCC TAAGAGGTCA GCAGTATGGT
    AGGAGCTGTG ATGTATGGAG TGTTGGCTGC GCCATTATAG AAATGGCTTG TGCAAAACCA
    CCTTGGAATG CAGAAAAACA CTCCAATCAT CTCGCCTTGA TATTTAAGAT TGCTAGCGCA
    ACTACTGCAC CGTCCATCCC GTCACACCTG TCCCCGGGTC TGCGCGACGT GGCGCTGCGC
    TGCTTAGAAC TTCAGCCTCA GGACCGGCCT CCGTCCAGAG AGCTGCTGAA ACATCCGGTC
    TTCCGTACCA CGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu14906
    Clone ID Related Accession (Same CDS sequence) NM_011945.2 , NM_011945.3
    Accession Version NM_011945.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC118704.17. On May 25, 2006 this sequence version replaced NM_011945.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF117340.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCGG CGGCGGGCGA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGCGGCGGCG 
    AGTCCCGAGG CGGGCGGCGG CGGCGGAGGA GGAGGAGCTC TCCAGGGAAG CGGCGCGCCC
    GCAGCGGGCG CGGCGGGGCT GCTGCGGGAG CCTGGCAGCG CGGGCCGCGA GCGCGCGGAC
    TGGCGGCGGC GGCAGCTGCG CAAAGTGCGG AGTGTGGAGC TGGACCAGCT GCCGGAGCAG
    CCGCTCTTCC TCGCCGCCGC CTCGCCGCCC TGCCCATCTA CTTCCCCGTC GCCGGAGCCC
    GCGGACGCGG CTGCAGGAGC GAGTCGCTTC CAGCCCGCGG CGGGACCGCC ACCCCCGGGA
    GCGGCGAGTC GCTGCGGCTC CCACTCTGCC GAGCTGGCGG CCGCGCGGGA CAGCGGCGCC
    CGGAGCCCCG CGGGGGCCGA GCCGCCCTCT GCAGCGGCCC CCTCCGGTCG AGAGATGGAG
    AATAAAGAAA CCCTCAAAGG ACTGCACAAG ATGGAGGATC GCCCGGAGGA GAGAATGATC
    CGGGAGAAGC TCAAGGCGAC CTGTATGCCC GCCTGGAAGC ACGAGTGGTT GGAGAGGAGG
    AACAGGAGAG GCCCTGTGGT GGTGAAGCCA ATCCCTATTA AAGGAGATGG ATCTGAAATG
    AATAACTTGG CAGCTGAGCC CCAAGGAGAG GGCCAGGCAG GTTCCGCTGC ACCAGCCCCC
    AAGGGCCGAC GAAGCCCATC TCCTGGCAGC TCTCCGTCAG GGCGCTCGGT GAAGCCGGAA
    TCCCCAGGAG TGAGACGGAA ACGAGTGTCC CCGGTGCCTT TCCAGAGTGG CAGAATCACA
    CCACCCCGAA GAGCCCCGTC ACCGGATGGC TTCTCCCCGT ACAGCCCAGA GGAGACGAGC
    CGCCGCGTGA ACAAAGTGAT GAGAGCCAGG CTGTACCTGC TGCAGCAGAT AGGACCCAAC
    TCTTTCCTGA TTGGAGGAGA CAGTCCAGAC AATAAATACC GGGTGTTTAT TGGGCCACAG
    AACTGCAGCT GTGGGCGTGG AGCATTCTGT ATTCACCTCT TGTTTGTCAT GCTCCGGGTG
    TTTCAGCTAG AACCCTCTGA CCCCATGTTA TGGAGAAAAA CTTTAAAAAA TTTCGAGGTT
    GAGAGTTTGT TCCAGAAATA CCACAGTAGG CGTAGCTCGA GAATCAAAGC TCCATCCCGG
    AACACCATCC AGAAGTTTGT GTCACGCATG TCAAATTGTC ACACACTGTC ATCGTCTAGC
    ACATCCACAT CTAGTTCAGA AAACAGCATC AAGGATGAAG AGGAGCAGAT GTGTCCCATC
    TGCTTGCTGG GCATGCTGGA TGAGGAGAGC CTGACTGTGT GTGAAGATGG CTGCAGGAAC
    AAGCTGCACC ACCATTGCAT GTCCATCTGG GCGGAAGAGT GTAGAAGAAA TAGAGAGCCT
    TTAATATGTC CCCTTTGTAG ATCTAAGTGG AGATCCCATG ACTTCTACAG CCATGAGTTA
    TCAAGCCCCG TGGAGTCCCC CACCTCCCTG CGAGCTGTCC AGCAGCCATC CTCCCCACAG
    CAGCCCGTGG CCGGATCACA GCGAAGGAAT CAGGAGAGCA GTTTTAACCT TACTCATTTT
    GGAACCCAGC AGATTCCTTC CGCTTACAAA GATTTGGCCG AGCCATGGAT TCAGGTGTTT
    GGAATGGAAC TCGTTGGCTG CTTATTCTCT AGAAACTGGA ACGTAAGGGA AATGGCCCTT
    AGGCGTCTTT CCCACGACGT TAGTGGGGCC CTGTTGTTGG CAAACGGGGA GAGCACTGGA
    AACTCTGGAG GCGGCAGTGG GGGCAGCTTA AGCGCGGGAG CGGCCAGCGG GTCCTCCCAG
    CCCAGCATCT CAGGGGATGT GGTGGAGGCG TGCTGCAGTG TCCTGTCTAT AGTCTGCGCT
    GACCCTGTCT ACAAAGTGTA CGTTGCTGCT TTAAAAACAT TGAGAGCCAT GCTGGTATAC
    ACTCCTTGCC ACAGTCTGGC AGAAAGAATC AAACTTCAGA GACTCCTCCG GCCAGTTGTA
    GACACTATCC TTGTCAAGTG TGCAGATGCC AACAGCCGCA CGAGTCAGCT GTCCATATCT
    ACAGTGCTGG AACTCTGCAA GGGCCAAGCA GGAGAGCTGG CGGTTGGGAG AGAAATACTT
    AAAGCTGGGT CCATCGGGGT TGGTGGTGTC GATTACGTCT TAAGTTGTAT CCTTGGAAAC
    CAAGCTGAAT CAAACAACTG GCAAGAACTG CTGGGTCGCC TCTGTCTTAT AGACAGGTTG
    CTGTTGGAAT TTCCTGCTGA ATTCTATCCT CATATTGTCA GTACTGATGT CTCACAAGCT
    GAGCCTGTTG AAATCAGGTA CAAGAAGCTG CTCTCCCTCT TAACCTTTGC CTTGCAATCC
    ATTGACAATT CCCACTCGAT GGTTGGCAAG CTCTCTCGGA GGATATATCT GAGCTCTGCC
    AGGATGGTGA CCGCAGTGCC CGCTGTGTTT TCCAAGCTGG TAACCATGCT TAATGCTTCT
    GGCTCCACCC ACTTCACCAG GATGCGCCGG CGTCTGATGG CTATCGCGGA TGAGGTAGAA
    ATTGCCGAGG TCATCCAGCT GGGTGTGGAG GACACTGTGG ATGGGCATCA GGACAGCTTA
    CAGGTGCTGG CCCCCGCCAG CTGTCTAGAA AACAGCTCCC TTGAGCACAC AGTCCATAGA
    GAGAAAACTG GAAAAGGACT AAGTGCTACG AGACTGAGTG CCAGCTCGGA GGACATTTCT
    GACAGACTGG CCGGCGTCTC TGTAGGACTT CCCAGCTCAA CAACAACAGA ACAACCAAAG
    CCAGCGGTTC AAACAAAAGG CAGACCCCAC AGTCAGTGTT TGAACTCCTC CCCTTTGTCT
    CATGCTCAAT TAATGTTCCC AGCACCATCA GCCCCTTGTT CCTCTGCCCC GTCTGTCCCA
    GATATTTCTA AGCACAGACC CCAGGCATTT GTTCCCTGCA AAATACCTTC CGCATCTCCT
    CAGACACAGC GCAAGTTCTC TCTACAATTC CAGAGGAACT GCTCTGAACA CCGAGACTCA
    GACCAGCTCT CCCCAGTCTT CACTCAGTCA AGGCCCCCAC CCTCCAGTAA CATACACAGG
    CCAAAGCCAT CCCGACCCGT TCCGGGCAGT ACAAGCAAAC TAGGGGACGC CACAAAAAGT
    AGCATGACAC TTGATCTGGG CAGTGCTTCC AGGTGTGACG ACAGCTTTGG CGGCGGCGGC
    AACAGTGGCA ACGCCGTCAT ACCCAGCGAA GAGACAGTGT TCACGCCGGT GGAGGACAAG
    TGCAGGTTAG ATGTGAACAC CGAGCTCAAC TCCAGCATCG AGGACCTTCT TGAAGCATCC
    ATGCCTTCAA GTGACACGAC AGTCACTTTC AAGTCCGAAG TCGCCGTCCT CTCTCCGGAA
    AAGGCCGAAA ATGACGACAC CTACAAAGAC GACGTCAATC ATAATCAAAA GTGCAAAGAA
    AAGATGGAAG CTGAAGAGGA GGAGGCTTTA GCGATCGCCA TGGCGATGTC AGCGTCTCAG
    GATGCCCTCC CCATCGTCCC TCAGCTGCAG GTGGAAAATG GAGAAGATAT TATCATCATT
    CAGCAGGACA CACCAGAAAC TCTTCCAGGA CATACCAAAG CGAAACAGCC TTACAGAGAA
    GACGCTGAGT GGCTGAAAGG CCAGCAGATA GGCCTCGGAG CATTTTCTTC CTGTTACCAA
    GCACAGGATG TGGGGACTGG GACTTTAATG GCTGTGAAAC AGGTGACGTA CGTCAGAAAC
    ACATCCTCCG AGCAGGAGGA GGTGGTGGAA GCGTTGAGGG AAGAGATCCG GATGATGGGT
    CACCTCAACC ATCCAAACAT CATCCGGATG CTGGGGGCCA CGTGCGAGAA GAGCAACTAC
    AACCTCTTCA TTGAGTGGAT GGCGGGAGGA TCTGTGGCTC ACCTCTTGAG TAAATACGGA
    GCTTTCAAGG AGTCAGTCGT CATTAACTAC ACTGAGCAGT TACTCCGTGG CCTTTCCTAT
    CTCCACGAGA ACCAGATCAT TCACAGAGAC GTCAAAGGTG CCAACCTGCT CATTGACAGC
    ACCGGTCAGA GGCTGAGAAT TGCAGACTTT GGAGCTGCTG CCAGGTTGGC ATCAAAAGGA
    ACCGGTGCAG GAGAGTTCCA GGGACAGTTA CTGGGGACAA TTGCATTCAT GGCGCCTGAG
    GTCCTAAGAG GTCAGCAGTA TGGTAGGAGC TGTGATGTAT GGAGTGTTGG CTGCGCCATT
    ATAGAAATGG CTTGTGCAAA ACCACCTTGG AATGCAGAAA AACACTCCAA TCATCTCGCC
    TTGATATTTA AGATTGCTAG CGCAACTACT GCACCGTCCA TCCCGTCACA CCTGTCCCCG
    GGTCTGCGCG ACGTGGCGCT GCGCTGCTTA GAACTTCAGC CTCAGGACCG GCCTCCGTCC
    AGAGAGCTGC TGAAACATCC GGTCTTCCGT ACCACGTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_036075.2
    CDS15..4496
    Translation

    Target ORF information:

    RefSeq Version NM_011945.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_011945.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCGG CGGCGGGCGA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGCGGCGGCG 
    AGTCCCGAGG CGGGCGGCGG CGGCGGAGGA GGAGGAGCTC TCCAGGGAAG CGGCGCGCCC
    GCAGCGGGCG CGGCGGGGCT GCTGCGGGAG CCTGGCAGCG CGGGCCGCGA GCGCGCGGAC
    TGGCGGCGGC GGCAGCTGCG CAAAGTGCGG AGTGTGGAGC TGGACCAGCT GCCGGAGCAG
    CCGCTCTTCC TCGCCGCCGC CTCGCCGCCC TGCCCATCTA CTTCCCCGTC GCCGGAGCCC
    GCGGACGCGG CTGCAGGAGC GAGTCGCTTC CAGCCCGCGG CGGGACCGCC ACCCCCGGGA
    GCGGCGAGTC GCTGCGGCTC CCACTCTGCC GAGCTGGCGG CCGCGCGGGA CAGCGGCGCC
    CGGAGCCCCG CGGGGGCCGA GCCGCCCTCT GCAGCGGCCC CCTCCGGTCG AGAGATGGAG
    AATAAAGAAA CCCTCAAAGG ACTGCACAAG ATGGAGGATC GCCCGGAGGA GAGAATGATC
    CGGGAGAAGC TCAAGGCGAC CTGTATGCCC GCCTGGAAGC ACGAGTGGTT GGAGAGGAGG
    AACAGGAGAG GCCCTGTGGT GGTGAAGCCA ATCCCTATTA AAGGAGATGG ATCTGAAATG
    AATAACTTGG CAGCTGAGCC CCAAGGAGAG GGCCAGGCAG GTTCCGCTGC ACCAGCCCCC
    AAGGGCCGAC GAAGCCCATC TCCTGGCAGC TCTCCGTCAG GGCGCTCGGT GAAGCCGGAA
    TCCCCAGGAG TGAGACGGAA ACGAGTGTCC CCGGTGCCTT TCCAGAGTGG CAGAATCACA
    CCACCCCGAA GAGCCCCGTC ACCGGATGGC TTCTCCCCGT ACAGCCCAGA GGAGACGAGC
    CGCCGCGTGA ACAAAGTGAT GAGAGCCAGG CTGTACCTGC TGCAGCAGAT AGGACCCAAC
    TCTTTCCTGA TTGGAGGAGA CAGTCCAGAC AATAAATACC GGGTGTTTAT TGGGCCACAG
    AACTGCAGCT GTGGGCGTGG AGCATTCTGT ATTCACCTCT TGTTTGTCAT GCTCCGGGTG
    TTTCAGCTAG AACCCTCTGA CCCCATGTTA TGGAGAAAAA CTTTAAAAAA TTTCGAGGTT
    GAGAGTTTGT TCCAGAAATA CCACAGTAGG CGTAGCTCGA GAATCAAAGC TCCATCCCGG
    AACACCATCC AGAAGTTTGT GTCACGCATG TCAAATTGTC ACACACTGTC ATCGTCTAGC
    ACATCCACAT CTAGTTCAGA AAACAGCATC AAGGATGAAG AGGAGCAGAT GTGTCCCATC
    TGCTTGCTGG GCATGCTGGA TGAGGAGAGC CTGACTGTGT GTGAAGATGG CTGCAGGAAC
    AAGCTGCACC ACCATTGCAT GTCCATCTGG GCGGAAGAGT GTAGAAGAAA TAGAGAGCCT
    TTAATATGTC CCCTTTGTAG ATCTAAGTGG AGATCCCATG ACTTCTACAG CCATGAGTTA
    TCAAGCCCCG TGGAGTCCCC CACCTCCCTG CGAGCTGTCC AGCAGCCATC CTCCCCACAG
    CAGCCCGTGG CCGGATCACA GCGAAGGAAT CAGGAGAGCA GTTTTAACCT TACTCATTTT
    GGAACCCAGC AGATTCCTTC CGCTTACAAA GATTTGGCCG AGCCATGGAT TCAGGTGTTT
    GGAATGGAAC TCGTTGGCTG CTTATTCTCT AGAAACTGGA ACGTAAGGGA AATGGCCCTT
    AGGCGTCTTT CCCACGACGT TAGTGGGGCC CTGTTGTTGG CAAACGGGGA GAGCACTGGA
    AACTCTGGAG GCGGCAGTGG GGGCAGCTTA AGCGCGGGAG CGGCCAGCGG GTCCTCCCAG
    CCCAGCATCT CAGGGGATGT GGTGGAGGCG TGCTGCAGTG TCCTGTCTAT AGTCTGCGCT
    GACCCTGTCT ACAAAGTGTA CGTTGCTGCT TTAAAAACAT TGAGAGCCAT GCTGGTATAC
    ACTCCTTGCC ACAGTCTGGC AGAAAGAATC AAACTTCAGA GACTCCTCCG GCCAGTTGTA
    GACACTATCC TTGTCAAGTG TGCAGATGCC AACAGCCGCA CGAGTCAGCT GTCCATATCT
    ACAGTGCTGG AACTCTGCAA GGGCCAAGCA GGAGAGCTGG CGGTTGGGAG AGAAATACTT
    AAAGCTGGGT CCATCGGGGT TGGTGGTGTC GATTACGTCT TAAGTTGTAT CCTTGGAAAC
    CAAGCTGAAT CAAACAACTG GCAAGAACTG CTGGGTCGCC TCTGTCTTAT AGACAGGTTG
    CTGTTGGAAT TTCCTGCTGA ATTCTATCCT CATATTGTCA GTACTGATGT CTCACAAGCT
    GAGCCTGTTG AAATCAGGTA CAAGAAGCTG CTCTCCCTCT TAACCTTTGC CTTGCAATCC
    ATTGACAATT CCCACTCGAT GGTTGGCAAG CTCTCTCGGA GGATATATCT GAGCTCTGCC
    AGGATGGTGA CCGCAGTGCC CGCTGTGTTT TCCAAGCTGG TAACCATGCT TAATGCTTCT
    GGCTCCACCC ACTTCACCAG GATGCGCCGG CGTCTGATGG CTATCGCGGA TGAGGTAGAA
    ATTGCCGAGG TCATCCAGCT GGGTGTGGAG GACACTGTGG ATGGGCATCA GGACAGCTTA
    CAGGTGCTGG CCCCCGCCAG CTGTCTAGAA AACAGCTCCC TTGAGCACAC AGTCCATAGA
    GAGAAAACTG GAAAAGGACT AAGTGCTACG AGACTGAGTG CCAGCTCGGA GGACATTTCT
    GACAGACTGG CCGGCGTCTC TGTAGGACTT CCCAGCTCAA CAACAACAGA ACAACCAAAG
    CCAGCGGTTC AAACAAAAGG CAGACCCCAC AGTCAGTGTT TGAACTCCTC CCCTTTGTCT
    CATGCTCAAT TAATGTTCCC AGCACCATCA GCCCCTTGTT CCTCTGCCCC GTCTGTCCCA
    GATATTTCTA AGCACAGACC CCAGGCATTT GTTCCCTGCA AAATACCTTC CGCATCTCCT
    CAGACACAGC GCAAGTTCTC TCTACAATTC CAGAGGAACT GCTCTGAACA CCGAGACTCA
    GACCAGCTCT CCCCAGTCTT CACTCAGTCA AGGCCCCCAC CCTCCAGTAA CATACACAGG
    CCAAAGCCAT CCCGACCCGT TCCGGGCAGT ACAAGCAAAC TAGGGGACGC CACAAAAAGT
    AGCATGACAC TTGATCTGGG CAGTGCTTCC AGGTGTGACG ACAGCTTTGG CGGCGGCGGC
    AACAGTGGCA ACGCCGTCAT ACCCAGCGAA GAGACAGTGT TCACGCCGGT GGAGGACAAG
    TGCAGGTTAG ATGTGAACAC CGAGCTCAAC TCCAGCATCG AGGACCTTCT TGAAGCATCC
    ATGCCTTCAA GTGACACGAC AGTCACTTTC AAGTCCGAAG TCGCCGTCCT CTCTCCGGAA
    AAGGCCGAAA ATGACGACAC CTACAAAGAC GACGTCAATC ATAATCAAAA GTGCAAAGAA
    AAGATGGAAG CTGAAGAGGA GGAGGCTTTA GCGATCGCCA TGGCGATGTC AGCGTCTCAG
    GATGCCCTCC CCATCGTCCC TCAGCTGCAG GTGGAAAATG GAGAAGATAT TATCATCATT
    CAGCAGGACA CACCAGAAAC TCTTCCAGGA CATACCAAAG CGAAACAGCC TTACAGAGAA
    GACGCTGAGT GGCTGAAAGG CCAGCAGATA GGCCTCGGAG CATTTTCTTC CTGTTACCAA
    GCACAGGATG TGGGGACTGG GACTTTAATG GCTGTGAAAC AGGTGACGTA CGTCAGAAAC
    ACATCCTCCG AGCAGGAGGA GGTGGTGGAA GCGTTGAGGG AAGAGATCCG GATGATGGGT
    CACCTCAACC ATCCAAACAT CATCCGGATG CTGGGGGCCA CGTGCGAGAA GAGCAACTAC
    AACCTCTTCA TTGAGTGGAT GGCGGGAGGA TCTGTGGCTC ACCTCTTGAG TAAATACGGA
    GCTTTCAAGG AGTCAGTCGT CATTAACTAC ACTGAGCAGT TACTCCGTGG CCTTTCCTAT
    CTCCACGAGA ACCAGATCAT TCACAGAGAC GTCAAAGGTG CCAACCTGCT CATTGACAGC
    ACCGGTCAGA GGCTGAGAAT TGCAGACTTT GGAGCTGCTG CCAGGTTGGC ATCAAAAGGA
    ACCGGTGCAG GAGAGTTCCA GGGACAGTTA CTGGGGACAA TTGCATTCAT GGCGCCTGAG
    GTCCTAAGAG GTCAGCAGTA TGGTAGGAGC TGTGATGTAT GGAGTGTTGG CTGCGCCATT
    ATAGAAATGG CTTGTGCAAA ACCACCTTGG AATGCAGAAA AACACTCCAA TCATCTCGCC
    TTGATATTTA AGATTGCTAG CGCAACTACT GCACCGTCCA TCCCGTCACA CCTGTCCCCG
    GGTCTGCGCG ACGTGGCGCT GCGCTGCTTA GAACTTCAGC CTCAGGACCG GCCTCCGTCC
    AGAGAGCTGC TGAAACATCC GGTCTTCCGT ACCACGTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu14906
    Clone ID Related Accession (Same CDS sequence) NM_011945.2 , NM_011945.3
    Accession Version NM_011945.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118704.17. On Apr 13, 2018 this sequence version replaced NM_011945.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF117340.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCGG CGGCGGGCGA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGCGGCGGCG 
    AGTCCCGAGG CGGGCGGCGG CGGCGGAGGA GGAGGAGCTC TCCAGGGAAG CGGCGCGCCC
    GCAGCGGGCG CGGCGGGGCT GCTGCGGGAG CCTGGCAGCG CGGGCCGCGA GCGCGCGGAC
    TGGCGGCGGC GGCAGCTGCG CAAAGTGCGG AGTGTGGAGC TGGACCAGCT GCCGGAGCAG
    CCGCTCTTCC TCGCCGCCGC CTCGCCGCCC TGCCCATCTA CTTCCCCGTC GCCGGAGCCC
    GCGGACGCGG CTGCAGGAGC GAGTCGCTTC CAGCCCGCGG CGGGACCGCC ACCCCCGGGA
    GCGGCGAGTC GCTGCGGCTC CCACTCTGCC GAGCTGGCGG CCGCGCGGGA CAGCGGCGCC
    CGGAGCCCCG CGGGGGCCGA GCCGCCCTCT GCAGCGGCCC CCTCCGGTCG AGAGATGGAG
    AATAAAGAAA CCCTCAAAGG ACTGCACAAG ATGGAGGATC GCCCGGAGGA GAGAATGATC
    CGGGAGAAGC TCAAGGCGAC CTGTATGCCC GCCTGGAAGC ACGAGTGGTT GGAGAGGAGG
    AACAGGAGAG GCCCTGTGGT GGTGAAGCCA ATCCCTATTA AAGGAGATGG ATCTGAAATG
    AATAACTTGG CAGCTGAGCC CCAAGGAGAG GGCCAGGCAG GTTCCGCTGC ACCAGCCCCC
    AAGGGCCGAC GAAGCCCATC TCCTGGCAGC TCTCCGTCAG GGCGCTCGGT GAAGCCGGAA
    TCCCCAGGAG TGAGACGGAA ACGAGTGTCC CCGGTGCCTT TCCAGAGTGG CAGAATCACA
    CCACCCCGAA GAGCCCCGTC ACCGGATGGC TTCTCCCCGT ACAGCCCAGA GGAGACGAGC
    CGCCGCGTGA ACAAAGTGAT GAGAGCCAGG CTGTACCTGC TGCAGCAGAT AGGACCCAAC
    TCTTTCCTGA TTGGAGGAGA CAGTCCAGAC AATAAATACC GGGTGTTTAT TGGGCCACAG
    AACTGCAGCT GTGGGCGTGG AGCATTCTGT ATTCACCTCT TGTTTGTCAT GCTCCGGGTG
    TTTCAGCTAG AACCCTCTGA CCCCATGTTA TGGAGAAAAA CTTTAAAAAA TTTCGAGGTT
    GAGAGTTTGT TCCAGAAATA CCACAGTAGG CGTAGCTCGA GAATCAAAGC TCCATCCCGG
    AACACCATCC AGAAGTTTGT GTCACGCATG TCAAATTGTC ACACACTGTC ATCGTCTAGC
    ACATCCACAT CTAGTTCAGA AAACAGCATC AAGGATGAAG AGGAGCAGAT GTGTCCCATC
    TGCTTGCTGG GCATGCTGGA TGAGGAGAGC CTGACTGTGT GTGAAGATGG CTGCAGGAAC
    AAGCTGCACC ACCATTGCAT GTCCATCTGG GCGGAAGAGT GTAGAAGAAA TAGAGAGCCT
    TTAATATGTC CCCTTTGTAG ATCTAAGTGG AGATCCCATG ACTTCTACAG CCATGAGTTA
    TCAAGCCCCG TGGAGTCCCC CACCTCCCTG CGAGCTGTCC AGCAGCCATC CTCCCCACAG
    CAGCCCGTGG CCGGATCACA GCGAAGGAAT CAGGAGAGCA GTTTTAACCT TACTCATTTT
    GGAACCCAGC AGATTCCTTC CGCTTACAAA GATTTGGCCG AGCCATGGAT TCAGGTGTTT
    GGAATGGAAC TCGTTGGCTG CTTATTCTCT AGAAACTGGA ACGTAAGGGA AATGGCCCTT
    AGGCGTCTTT CCCACGACGT TAGTGGGGCC CTGTTGTTGG CAAACGGGGA GAGCACTGGA
    AACTCTGGAG GCGGCAGTGG GGGCAGCTTA AGCGCGGGAG CGGCCAGCGG GTCCTCCCAG
    CCCAGCATCT CAGGGGATGT GGTGGAGGCG TGCTGCAGTG TCCTGTCTAT AGTCTGCGCT
    GACCCTGTCT ACAAAGTGTA CGTTGCTGCT TTAAAAACAT TGAGAGCCAT GCTGGTATAC
    ACTCCTTGCC ACAGTCTGGC AGAAAGAATC AAACTTCAGA GACTCCTCCG GCCAGTTGTA
    GACACTATCC TTGTCAAGTG TGCAGATGCC AACAGCCGCA CGAGTCAGCT GTCCATATCT
    ACAGTGCTGG AACTCTGCAA GGGCCAAGCA GGAGAGCTGG CGGTTGGGAG AGAAATACTT
    AAAGCTGGGT CCATCGGGGT TGGTGGTGTC GATTACGTCT TAAGTTGTAT CCTTGGAAAC
    CAAGCTGAAT CAAACAACTG GCAAGAACTG CTGGGTCGCC TCTGTCTTAT AGACAGGTTG
    CTGTTGGAAT TTCCTGCTGA ATTCTATCCT CATATTGTCA GTACTGATGT CTCACAAGCT
    GAGCCTGTTG AAATCAGGTA CAAGAAGCTG CTCTCCCTCT TAACCTTTGC CTTGCAATCC
    ATTGACAATT CCCACTCGAT GGTTGGCAAG CTCTCTCGGA GGATATATCT GAGCTCTGCC
    AGGATGGTGA CCGCAGTGCC CGCTGTGTTT TCCAAGCTGG TAACCATGCT TAATGCTTCT
    GGCTCCACCC ACTTCACCAG GATGCGCCGG CGTCTGATGG CTATCGCGGA TGAGGTAGAA
    ATTGCCGAGG TCATCCAGCT GGGTGTGGAG GACACTGTGG ATGGGCATCA GGACAGCTTA
    CAGGTGCTGG CCCCCGCCAG CTGTCTAGAA AACAGCTCCC TTGAGCACAC AGTCCATAGA
    GAGAAAACTG GAAAAGGACT AAGTGCTACG AGACTGAGTG CCAGCTCGGA GGACATTTCT
    GACAGACTGG CCGGCGTCTC TGTAGGACTT CCCAGCTCAA CAACAACAGA ACAACCAAAG
    CCAGCGGTTC AAACAAAAGG CAGACCCCAC AGTCAGTGTT TGAACTCCTC CCCTTTGTCT
    CATGCTCAAT TAATGTTCCC AGCACCATCA GCCCCTTGTT CCTCTGCCCC GTCTGTCCCA
    GATATTTCTA AGCACAGACC CCAGGCATTT GTTCCCTGCA AAATACCTTC CGCATCTCCT
    CAGACACAGC GCAAGTTCTC TCTACAATTC CAGAGGAACT GCTCTGAACA CCGAGACTCA
    GACCAGCTCT CCCCAGTCTT CACTCAGTCA AGGCCCCCAC CCTCCAGTAA CATACACAGG
    CCAAAGCCAT CCCGACCCGT TCCGGGCAGT ACAAGCAAAC TAGGGGACGC CACAAAAAGT
    AGCATGACAC TTGATCTGGG CAGTGCTTCC AGGTGTGACG ACAGCTTTGG CGGCGGCGGC
    AACAGTGGCA ACGCCGTCAT ACCCAGCGAA GAGACAGTGT TCACGCCGGT GGAGGACAAG
    TGCAGGTTAG ATGTGAACAC CGAGCTCAAC TCCAGCATCG AGGACCTTCT TGAAGCATCC
    ATGCCTTCAA GTGACACGAC AGTCACTTTC AAGTCCGAAG TCGCCGTCCT CTCTCCGGAA
    AAGGCCGAAA ATGACGACAC CTACAAAGAC GACGTCAATC ATAATCAAAA GTGCAAAGAA
    AAGATGGAAG CTGAAGAGGA GGAGGCTTTA GCGATCGCCA TGGCGATGTC AGCGTCTCAG
    GATGCCCTCC CCATCGTCCC TCAGCTGCAG GTGGAAAATG GAGAAGATAT TATCATCATT
    CAGCAGGACA CACCAGAAAC TCTTCCAGGA CATACCAAAG CGAAACAGCC TTACAGAGAA
    GACGCTGAGT GGCTGAAAGG CCAGCAGATA GGCCTCGGAG CATTTTCTTC CTGTTACCAA
    GCACAGGATG TGGGGACTGG GACTTTAATG GCTGTGAAAC AGGTGACGTA CGTCAGAAAC
    ACATCCTCCG AGCAGGAGGA GGTGGTGGAA GCGTTGAGGG AAGAGATCCG GATGATGGGT
    CACCTCAACC ATCCAAACAT CATCCGGATG CTGGGGGCCA CGTGCGAGAA GAGCAACTAC
    AACCTCTTCA TTGAGTGGAT GGCGGGAGGA TCTGTGGCTC ACCTCTTGAG TAAATACGGA
    GCTTTCAAGG AGTCAGTCGT CATTAACTAC ACTGAGCAGT TACTCCGTGG CCTTTCCTAT
    CTCCACGAGA ACCAGATCAT TCACAGAGAC GTCAAAGGTG CCAACCTGCT CATTGACAGC
    ACCGGTCAGA GGCTGAGAAT TGCAGACTTT GGAGCTGCTG CCAGGTTGGC ATCAAAAGGA
    ACCGGTGCAG GAGAGTTCCA GGGACAGTTA CTGGGGACAA TTGCATTCAT GGCGCCTGAG
    GTCCTAAGAG GTCAGCAGTA TGGTAGGAGC TGTGATGTAT GGAGTGTTGG CTGCGCCATT
    ATAGAAATGG CTTGTGCAAA ACCACCTTGG AATGCAGAAA AACACTCCAA TCATCTCGCC
    TTGATATTTA AGATTGCTAG CGCAACTACT GCACCGTCCA TCCCGTCACA CCTGTCCCCG
    GGTCTGCGCG ACGTGGCGCT GCGCTGCTTA GAACTTCAGC CTCAGGACCG GCCTCCGTCC
    AGAGAGCTGC TGAAACATCC GGTCTTCCGT ACCACGTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_036075.2
    CDS25..4506
    Translation

    Target ORF information:

    RefSeq Version NM_011945.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase 1 (Map3k1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_011945.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCGGCGG CGGCGGGCGA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGCGGCGGCG 
    AGTCCCGAGG CGGGCGGCGG CGGCGGAGGA GGAGGAGCTC TCCAGGGAAG CGGCGCGCCC
    GCAGCGGGCG CGGCGGGGCT GCTGCGGGAG CCTGGCAGCG CGGGCCGCGA GCGCGCGGAC
    TGGCGGCGGC GGCAGCTGCG CAAAGTGCGG AGTGTGGAGC TGGACCAGCT GCCGGAGCAG
    CCGCTCTTCC TCGCCGCCGC CTCGCCGCCC TGCCCATCTA CTTCCCCGTC GCCGGAGCCC
    GCGGACGCGG CTGCAGGAGC GAGTCGCTTC CAGCCCGCGG CGGGACCGCC ACCCCCGGGA
    GCGGCGAGTC GCTGCGGCTC CCACTCTGCC GAGCTGGCGG CCGCGCGGGA CAGCGGCGCC
    CGGAGCCCCG CGGGGGCCGA GCCGCCCTCT GCAGCGGCCC CCTCCGGTCG AGAGATGGAG
    AATAAAGAAA CCCTCAAAGG ACTGCACAAG ATGGAGGATC GCCCGGAGGA GAGAATGATC
    CGGGAGAAGC TCAAGGCGAC CTGTATGCCC GCCTGGAAGC ACGAGTGGTT GGAGAGGAGG
    AACAGGAGAG GCCCTGTGGT GGTGAAGCCA ATCCCTATTA AAGGAGATGG ATCTGAAATG
    AATAACTTGG CAGCTGAGCC CCAAGGAGAG GGCCAGGCAG GTTCCGCTGC ACCAGCCCCC
    AAGGGCCGAC GAAGCCCATC TCCTGGCAGC TCTCCGTCAG GGCGCTCGGT GAAGCCGGAA
    TCCCCAGGAG TGAGACGGAA ACGAGTGTCC CCGGTGCCTT TCCAGAGTGG CAGAATCACA
    CCACCCCGAA GAGCCCCGTC ACCGGATGGC TTCTCCCCGT ACAGCCCAGA GGAGACGAGC
    CGCCGCGTGA ACAAAGTGAT GAGAGCCAGG CTGTACCTGC TGCAGCAGAT AGGACCCAAC
    TCTTTCCTGA TTGGAGGAGA CAGTCCAGAC AATAAATACC GGGTGTTTAT TGGGCCACAG
    AACTGCAGCT GTGGGCGTGG AGCATTCTGT ATTCACCTCT TGTTTGTCAT GCTCCGGGTG
    TTTCAGCTAG AACCCTCTGA CCCCATGTTA TGGAGAAAAA CTTTAAAAAA TTTCGAGGTT
    GAGAGTTTGT TCCAGAAATA CCACAGTAGG CGTAGCTCGA GAATCAAAGC TCCATCCCGG
    AACACCATCC AGAAGTTTGT GTCACGCATG TCAAATTGTC ACACACTGTC ATCGTCTAGC
    ACATCCACAT CTAGTTCAGA AAACAGCATC AAGGATGAAG AGGAGCAGAT GTGTCCCATC
    TGCTTGCTGG GCATGCTGGA TGAGGAGAGC CTGACTGTGT GTGAAGATGG CTGCAGGAAC
    AAGCTGCACC ACCATTGCAT GTCCATCTGG GCGGAAGAGT GTAGAAGAAA TAGAGAGCCT
    TTAATATGTC CCCTTTGTAG ATCTAAGTGG AGATCCCATG ACTTCTACAG CCATGAGTTA
    TCAAGCCCCG TGGAGTCCCC CACCTCCCTG CGAGCTGTCC AGCAGCCATC CTCCCCACAG
    CAGCCCGTGG CCGGATCACA GCGAAGGAAT CAGGAGAGCA GTTTTAACCT TACTCATTTT
    GGAACCCAGC AGATTCCTTC CGCTTACAAA GATTTGGCCG AGCCATGGAT TCAGGTGTTT
    GGAATGGAAC TCGTTGGCTG CTTATTCTCT AGAAACTGGA ACGTAAGGGA AATGGCCCTT
    AGGCGTCTTT CCCACGACGT TAGTGGGGCC CTGTTGTTGG CAAACGGGGA GAGCACTGGA
    AACTCTGGAG GCGGCAGTGG GGGCAGCTTA AGCGCGGGAG CGGCCAGCGG GTCCTCCCAG
    CCCAGCATCT CAGGGGATGT GGTGGAGGCG TGCTGCAGTG TCCTGTCTAT AGTCTGCGCT
    GACCCTGTCT ACAAAGTGTA CGTTGCTGCT TTAAAAACAT TGAGAGCCAT GCTGGTATAC
    ACTCCTTGCC ACAGTCTGGC AGAAAGAATC AAACTTCAGA GACTCCTCCG GCCAGTTGTA
    GACACTATCC TTGTCAAGTG TGCAGATGCC AACAGCCGCA CGAGTCAGCT GTCCATATCT
    ACAGTGCTGG AACTCTGCAA GGGCCAAGCA GGAGAGCTGG CGGTTGGGAG AGAAATACTT
    AAAGCTGGGT CCATCGGGGT TGGTGGTGTC GATTACGTCT TAAGTTGTAT CCTTGGAAAC
    CAAGCTGAAT CAAACAACTG GCAAGAACTG CTGGGTCGCC TCTGTCTTAT AGACAGGTTG
    CTGTTGGAAT TTCCTGCTGA ATTCTATCCT CATATTGTCA GTACTGATGT CTCACAAGCT
    GAGCCTGTTG AAATCAGGTA CAAGAAGCTG CTCTCCCTCT TAACCTTTGC CTTGCAATCC
    ATTGACAATT CCCACTCGAT GGTTGGCAAG CTCTCTCGGA GGATATATCT GAGCTCTGCC
    AGGATGGTGA CCGCAGTGCC CGCTGTGTTT TCCAAGCTGG TAACCATGCT TAATGCTTCT
    GGCTCCACCC ACTTCACCAG GATGCGCCGG CGTCTGATGG CTATCGCGGA TGAGGTAGAA
    ATTGCCGAGG TCATCCAGCT GGGTGTGGAG GACACTGTGG ATGGGCATCA GGACAGCTTA
    CAGGTGCTGG CCCCCGCCAG CTGTCTAGAA AACAGCTCCC TTGAGCACAC AGTCCATAGA
    GAGAAAACTG GAAAAGGACT AAGTGCTACG AGACTGAGTG CCAGCTCGGA GGACATTTCT
    GACAGACTGG CCGGCGTCTC TGTAGGACTT CCCAGCTCAA CAACAACAGA ACAACCAAAG
    CCAGCGGTTC AAACAAAAGG CAGACCCCAC AGTCAGTGTT TGAACTCCTC CCCTTTGTCT
    CATGCTCAAT TAATGTTCCC AGCACCATCA GCCCCTTGTT CCTCTGCCCC GTCTGTCCCA
    GATATTTCTA AGCACAGACC CCAGGCATTT GTTCCCTGCA AAATACCTTC CGCATCTCCT
    CAGACACAGC GCAAGTTCTC TCTACAATTC CAGAGGAACT GCTCTGAACA CCGAGACTCA
    GACCAGCTCT CCCCAGTCTT CACTCAGTCA AGGCCCCCAC CCTCCAGTAA CATACACAGG
    CCAAAGCCAT CCCGACCCGT TCCGGGCAGT ACAAGCAAAC TAGGGGACGC CACAAAAAGT
    AGCATGACAC TTGATCTGGG CAGTGCTTCC AGGTGTGACG ACAGCTTTGG CGGCGGCGGC
    AACAGTGGCA ACGCCGTCAT ACCCAGCGAA GAGACAGTGT TCACGCCGGT GGAGGACAAG
    TGCAGGTTAG ATGTGAACAC CGAGCTCAAC TCCAGCATCG AGGACCTTCT TGAAGCATCC
    ATGCCTTCAA GTGACACGAC AGTCACTTTC AAGTCCGAAG TCGCCGTCCT CTCTCCGGAA
    AAGGCCGAAA ATGACGACAC CTACAAAGAC GACGTCAATC ATAATCAAAA GTGCAAAGAA
    AAGATGGAAG CTGAAGAGGA GGAGGCTTTA GCGATCGCCA TGGCGATGTC AGCGTCTCAG
    GATGCCCTCC CCATCGTCCC TCAGCTGCAG GTGGAAAATG GAGAAGATAT TATCATCATT
    CAGCAGGACA CACCAGAAAC TCTTCCAGGA CATACCAAAG CGAAACAGCC TTACAGAGAA
    GACGCTGAGT GGCTGAAAGG CCAGCAGATA GGCCTCGGAG CATTTTCTTC CTGTTACCAA
    GCACAGGATG TGGGGACTGG GACTTTAATG GCTGTGAAAC AGGTGACGTA CGTCAGAAAC
    ACATCCTCCG AGCAGGAGGA GGTGGTGGAA GCGTTGAGGG AAGAGATCCG GATGATGGGT
    CACCTCAACC ATCCAAACAT CATCCGGATG CTGGGGGCCA CGTGCGAGAA GAGCAACTAC
    AACCTCTTCA TTGAGTGGAT GGCGGGAGGA TCTGTGGCTC ACCTCTTGAG TAAATACGGA
    GCTTTCAAGG AGTCAGTCGT CATTAACTAC ACTGAGCAGT TACTCCGTGG CCTTTCCTAT
    CTCCACGAGA ACCAGATCAT TCACAGAGAC GTCAAAGGTG CCAACCTGCT CATTGACAGC
    ACCGGTCAGA GGCTGAGAAT TGCAGACTTT GGAGCTGCTG CCAGGTTGGC ATCAAAAGGA
    ACCGGTGCAG GAGAGTTCCA GGGACAGTTA CTGGGGACAA TTGCATTCAT GGCGCCTGAG
    GTCCTAAGAG GTCAGCAGTA TGGTAGGAGC TGTGATGTAT GGAGTGTTGG CTGCGCCATT
    ATAGAAATGG CTTGTGCAAA ACCACCTTGG AATGCAGAAA AACACTCCAA TCATCTCGCC
    TTGATATTTA AGATTGCTAG CGCAACTACT GCACCGTCCA TCCCGTCACA CCTGTCCCCG
    GGTCTGCGCG ACGTGGCGCT GCGCTGCTTA GAACTTCAGC CTCAGGACCG GCCTCCGTCC
    AGAGAGCTGC TGAAACATCC GGTCTTCCGT ACCACGTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.