TPCN1 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	TPCN1
Entrez Gene ID	252972
Full Name	two pore channel 1
Synonyms	5730403B01Rik,Tpc1,mKIAA1169
General protein information	Preferred Names two pore channel 1 Names two pore calcium channel protein 1 voltage-dependent calcium channel protein TPC1
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
XM_030254513.1	XP_030110373.1	two pore calcium channel protein 1 isoform X1
XM_011248201.3	XP_011246503.1	two pore calcium channel protein 1 isoform X1
XM_030254514.1	XP_030110374.1	two pore calcium channel protein 1 isoform X2
NM_145853.2	NP_665852.1	two pore calcium channel protein 1
XM_011248201.2	XP_011246503.1	two pore calcium channel protein 1 isoform X1
XM_011248201.1	XP_011246503.1	two pore calcium channel protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TPCN1	XP_003313998.1
Canis lupus familiaris (dog)	TPCN1	XP_534690.2
Bos taurus (cattle)	TPCN1	XP_002694563.1
Gallus gallus (chicken)	TPCN1	XP_004934547.1
Homo sapiens (human)	TPCN1	NP_001137291.1
Rattus norvegicus (Norway rat)	Tpcn1	NP_647548.2
Danio rerio (zebrafish)	tpcn1	XP_005165277.1
Xenopus tropicalis (tropical clawed frog)	tpcn1	XP_002934333.2
	TPCN1	XP_002798827.1
Mus musculus (house mouse)	Tpcn1	NP_665852.1

Gene Ontology
Bibliography

Related articles in PubMed

The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes.
Castonguay J, Orth JHC, M?ller T, Sleman F, Grimm C, Wahl-Schott C, Biel M, Mallmann RT, Bildl W, Schulte U, Klugbauer N
Scientific reports7(1)10038(2017 Aug)

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

Metabolic alterations derived from absence of Two-Pore Channel 1 at cardiac level.
Garcia-Rua V, Feijoo-Bandin S, Garcia-Vence M, Aragon-Herrera A, Bravo SB, Rodriguez-Penas D, Mosquera-Leal A, Lear PV, Parrington J, Alonso J, Rosello-Lleti E, Portoles M, Rivera M, Gonzalez-Juanatey JR, Lago F
Journal of biosciences41(4)643-658(2016 Dec)

Endolysosomal two-pore channels regulate autophagy in cardiomyocytes.
Garc?a-R?a V, Feij?o-Band?n S, Rodr?guez-Penas D, Mosquera-Leal A, Abu-Assi E, Beiras A, Mar?a Seoane L, Lear P, Parrington J, Portol?s M, Rosell?-Llet? E, Rivera M, Gualillo O, Parra V, Hill JA, Rothermel B, Gonz?lez-Juanatey JR, Lago F
The Journal of physiology594(11)3061-77(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results confirmed the altered expression of HFABP, a key fatty acid transport protein, and of enolase and PGK1, the key enzymes in the glycolytic process
Title: Metabolic alterations derived from absence of Two-Pore Channel 1 at cardiac level.

TPC1 and TPC2 were found to be essential for appropriate basal and induced autophagic flux in cardiomyocytes.
Title: Endolysosomal two-pore channels regulate autophagy in cardiomyocytes.

Mice lacking the endo-lysosomal TPC1 were protected from myocardial ischemia reperfusion injury.
Title: Inhibition of NAADP signalling on reperfusion protects the heart by preventing lethal calcium oscillations via two-pore channel 1 and opening of the mitochondrial permeability transition pore.

Genetic ablation of endolysosomal TPC1 or TPC2 channels attenuates glucose- and sulfonylurea-induced membrane currents, depolarization, cytoplasmic Ca2+ signals, and insulin secretion in pancreatic beta cells.
Title: Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) and Endolysosomal Two-pore Channels Modulate Membrane Excitability and Stimulus-Secretion Coupling in Mouse Pancreatic ? Cells.

It was concluded that Tpcn1/2(-/-) mice show mature-onset obesity due to reduced lipid availability and use, and a defect in beta-adrenergic receptor signaling, leading to impaired thermogenic activity, in brown adipose tissue.
Title: Absence of intracellular ion channels TPC1 and TPC2 leads to mature-onset obesity in male mice, due to impaired lipid availability for thermogenesis in brown adipose tissue.

The following TPCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TPCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12008	NM_145853.2 Latest version!	Mus musculus two pore channel 1 (Tpcn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu12008	XM_030254513.1 Latest version!	Mus musculus two pore channel 1 (Tpcn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu12008	XM_011248201.3 Latest version!	Mus musculus two pore channel 1 (Tpcn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu88912	XM_030254514.1 Latest version!	Mus musculus two pore channel 1 (Tpcn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu12008	XM_011248201.2	Mus musculus two pore channel 1 (Tpcn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu12008	XM_011248201.1	Mus musculus two pore channel 1 (Tpcn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TPCN1 ( NM_145853.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_665852.1 Mus musculus two pore channel 1 (Tpcn1), mRNA.

Price & Availability↑

CloneID	OMu12008
Clone ID Related Accession (Same CDS sequence)	XM_030254513.1 , XM_011248201.3 , NM_145853.2 , XM_011248201.2 , XM_011248201.1
Accession Version	NM_145853.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	two pore calcium channel protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC058951.1. On or before Dec 7, 2005 this sequence version replaced XM_915631.1, XM_923870.1, NM_145853.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058951.1, AK148129.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCGAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGGGGC 
AGCCACAGCA TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCGGAGCGGA CAGCCCCCCC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCCACAC GTTCGTCCGG CACAAACGTA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG CATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTACGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTC CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT TGCCATCCTC GGTTTCTACT TATTCTCCAC AAATCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTCGTTCT CCTGACCACA 
GCCAACTTTC CAGATGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCCGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCCTA CGGCCTGCTT GCCAGCCAAC GGAGGCCGGC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAGCCCC GGATGAGTGC AAGGGAACGC 
TTCCTGACTT TCAAGGCCTT GAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGGCAA AGAGAAACAG ACAGCATTGG 
TTTGATGAGC TCCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAACGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGTGGGAAT TTCACCTCAA AGCATGTGCC CTGGAGTTAC 
CTCGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACGGC ATTCGCCTTC 
CTGGGACTGC TCGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTTGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTCA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACAG TGGCCGACGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTCGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATCG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CAGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TCCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTGCTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGCCCGGC 
CCTGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACTGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_665852.1
CDS	244..2697
Translation	MAVSLDDDVPLILTLDEAESAPLPPSNSLGQEQLPSKNGGSHSIHNSQVPSLVSGADSPPSSPTGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPVLKLHTYVHATLELFALMVVVFELCMKLRWLGFHTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFSTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYGLLASQRRPAGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKRNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFTSKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFNGRLTPNCCNTSTVADAYRFINHTVGNKTKVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQDSEVDSGIVIEKEMSKEELMAVLELYREERGTSSDVTRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQKLRGSVPGPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	NM_145853.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus two pore channel 1 (Tpcn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145853.2

ORF Insert Sequence:

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCGAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGGGGC 
AGCCACAGCA TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCGGAGCGGA CAGCCCCCCC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCCACAC GTTCGTCCGG CACAAACGTA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG CATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTACGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTC CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT TGCCATCCTC GGTTTCTACT TATTCTCCAC AAATCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTCGTTCT CCTGACCACA 
GCCAACTTTC CAGATGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCCGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCCTA CGGCCTGCTT GCCAGCCAAC GGAGGCCGGC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAGCCCC GGATGAGTGC AAGGGAACGC 
TTCCTGACTT TCAAGGCCTT GAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGGCAA AGAGAAACAG ACAGCATTGG 
TTTGATGAGC TCCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAACGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGTGGGAAT TTCACCTCAA AGCATGTGCC CTGGAGTTAC 
CTCGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACGGC ATTCGCCTTC 
CTGGGACTGC TCGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTTGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTCA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACAG TGGCCGACGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTCGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATCG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CAGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TCCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTGCTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGCCCGGC 
CCTGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACTGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_030254513.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_030110373.1 Mus musculus two pore channel 1 (Tpcn1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu12008
Clone ID Related Accession (Same CDS sequence)	XM_030254513.1 , XM_011248201.3 , NM_145853.2 , XM_011248201.2 , XM_011248201.1
Accession Version	XM_030254513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	two pore calcium channel protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCGAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGGGGC 
AGCCACAGCA TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCGGAGCGGA CAGCCCCCCC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCCACAC GTTCGTCCGG CACAAACGTA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG CATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTACGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTC CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT TGCCATCCTC GGTTTCTACT TATTCTCCAC AAATCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTCGTTCT CCTGACCACA 
GCCAACTTTC CAGATGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCCGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCCTA CGGCCTGCTT GCCAGCCAAC GGAGGCCGGC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAGCCCC GGATGAGTGC AAGGGAACGC 
TTCCTGACTT TCAAGGCCTT GAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGGCAA AGAGAAACAG ACAGCATTGG 
TTTGATGAGC TCCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAACGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGTGGGAAT TTCACCTCAA AGCATGTGCC CTGGAGTTAC 
CTCGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACGGC ATTCGCCTTC 
CTGGGACTGC TCGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTTGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTCA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACAG TGGCCGACGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTCGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATCG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CAGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TCCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTGCTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGCCCGGC 
CCTGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACTGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110373.1
CDS	219..2672
Translation	MAVSLDDDVPLILTLDEAESAPLPPSNSLGQEQLPSKNGGSHSIHNSQVPSLVSGADSPPSSPTGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPVLKLHTYVHATLELFALMVVVFELCMKLRWLGFHTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFSTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYGLLASQRRPAGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKRNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFTSKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFNGRLTPNCCNTSTVADAYRFINHTVGNKTKVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQDSEVDSGIVIEKEMSKEELMAVLELYREERGTSSDVTRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQKLRGSVPGPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_030254513.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus two pore channel 1 (Tpcn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254513.1

ORF Insert Sequence:

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCGAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGGGGC 
AGCCACAGCA TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCGGAGCGGA CAGCCCCCCC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCCACAC GTTCGTCCGG CACAAACGTA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG CATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTACGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTC CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT TGCCATCCTC GGTTTCTACT TATTCTCCAC AAATCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTCGTTCT CCTGACCACA 
GCCAACTTTC CAGATGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCCGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCCTA CGGCCTGCTT GCCAGCCAAC GGAGGCCGGC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAGCCCC GGATGAGTGC AAGGGAACGC 
TTCCTGACTT TCAAGGCCTT GAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGGCAA AGAGAAACAG ACAGCATTGG 
TTTGATGAGC TCCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAACGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGTGGGAAT TTCACCTCAA AGCATGTGCC CTGGAGTTAC 
CTCGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACGGC ATTCGCCTTC 
CTGGGACTGC TCGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTTGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTCA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACAG TGGCCGACGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTCGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATCG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CAGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TCCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTGCTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGCCCGGC 
CCTGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACTGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_011248201.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011246503.1 Mus musculus two pore channel 1 (Tpcn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu12008
Clone ID Related Accession (Same CDS sequence)	XM_030254513.1 , XM_011248201.3 , NM_145853.2 , XM_011248201.2 , XM_011248201.1
Accession Version	XM_011248201.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	two pore calcium channel protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248201.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCGAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGGGGC 
AGCCACAGCA TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCGGAGCGGA CAGCCCCCCC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCCACAC GTTCGTCCGG CACAAACGTA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG CATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTACGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTC CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT TGCCATCCTC GGTTTCTACT TATTCTCCAC AAATCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTCGTTCT CCTGACCACA 
GCCAACTTTC CAGATGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCCGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCCTA CGGCCTGCTT GCCAGCCAAC GGAGGCCGGC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAGCCCC GGATGAGTGC AAGGGAACGC 
TTCCTGACTT TCAAGGCCTT GAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGGCAA AGAGAAACAG ACAGCATTGG 
TTTGATGAGC TCCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAACGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGTGGGAAT TTCACCTCAA AGCATGTGCC CTGGAGTTAC 
CTCGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACGGC ATTCGCCTTC 
CTGGGACTGC TCGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTTGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTCA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACAG TGGCCGACGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTCGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATCG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CAGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TCCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTGCTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGCCCGGC 
CCTGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACTGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246503.1
CDS	314..2767
Translation	MAVSLDDDVPLILTLDEAESAPLPPSNSLGQEQLPSKNGGSHSIHNSQVPSLVSGADSPPSSPTGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPVLKLHTYVHATLELFALMVVVFELCMKLRWLGFHTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFSTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYGLLASQRRPAGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKRNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFTSKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFNGRLTPNCCNTSTVADAYRFINHTVGNKTKVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQDSEVDSGIVIEKEMSKEELMAVLELYREERGTSSDVTRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQKLRGSVPGPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_011248201.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus two pore channel 1 (Tpcn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248201.3

ORF Insert Sequence:

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCGAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGGGGC 
AGCCACAGCA TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCGGAGCGGA CAGCCCCCCC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCCACAC GTTCGTCCGG CACAAACGTA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG CATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTACGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTC CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT TGCCATCCTC GGTTTCTACT TATTCTCCAC AAATCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTCGTTCT CCTGACCACA 
GCCAACTTTC CAGATGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCCGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCCTA CGGCCTGCTT GCCAGCCAAC GGAGGCCGGC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAGCCCC GGATGAGTGC AAGGGAACGC 
TTCCTGACTT TCAAGGCCTT GAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGGCAA AGAGAAACAG ACAGCATTGG 
TTTGATGAGC TCCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAACGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGTGGGAAT TTCACCTCAA AGCATGTGCC CTGGAGTTAC 
CTCGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACGGC ATTCGCCTTC 
CTGGGACTGC TCGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTTGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTCA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACAG TGGCCGACGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTCGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATCG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CAGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TCCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTGCTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGCCCGGC 
CCTGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACTGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_011248201.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011246503.1 Mus musculus two pore channel 1 (Tpcn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu12008
Clone ID Related Accession (Same CDS sequence)	XM_030254513.1 , XM_011248201.3 , NM_145853.2 , XM_011248201.2 , XM_011248201.1
Accession Version	XM_011248201.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	two pore calcium channel protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248201.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCGAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGGGGC 
AGCCACAGCA TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCGGAGCGGA CAGCCCCCCC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCCACAC GTTCGTCCGG CACAAACGTA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG CATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTACGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTC CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT TGCCATCCTC GGTTTCTACT TATTCTCCAC AAATCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTCGTTCT CCTGACCACA 
GCCAACTTTC CAGATGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCCGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCCTA CGGCCTGCTT GCCAGCCAAC GGAGGCCGGC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAGCCCC GGATGAGTGC AAGGGAACGC 
TTCCTGACTT TCAAGGCCTT GAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGGCAA AGAGAAACAG ACAGCATTGG 
TTTGATGAGC TCCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAACGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGTGGGAAT TTCACCTCAA AGCATGTGCC CTGGAGTTAC 
CTCGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACGGC ATTCGCCTTC 
CTGGGACTGC TCGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTTGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTCA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACAG TGGCCGACGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTCGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATCG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CAGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TCCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTGCTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGCCCGGC 
CCTGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACTGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246503.1
CDS	343..2796
Translation	MAVSLDDDVPLILTLDEAESAPLPPSNSLGQEQLPSKNGGSHSIHNSQVPSLVSGADSPPSSPTGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPVLKLHTYVHATLELFALMVVVFELCMKLRWLGFHTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFSTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYGLLASQRRPAGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKRNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFTSKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFNGRLTPNCCNTSTVADAYRFINHTVGNKTKVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQDSEVDSGIVIEKEMSKEELMAVLELYREERGTSSDVTRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQKLRGSVPGPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_011248201.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus two pore channel 1 (Tpcn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248201.2

ORF Insert Sequence:

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCGAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGGGGC 
AGCCACAGCA TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCGGAGCGGA CAGCCCCCCC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCCACAC GTTCGTCCGG CACAAACGTA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG CATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTACGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTC CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT TGCCATCCTC GGTTTCTACT TATTCTCCAC AAATCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTCGTTCT CCTGACCACA 
GCCAACTTTC CAGATGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCCGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCCTA CGGCCTGCTT GCCAGCCAAC GGAGGCCGGC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAGCCCC GGATGAGTGC AAGGGAACGC 
TTCCTGACTT TCAAGGCCTT GAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGGCAA AGAGAAACAG ACAGCATTGG 
TTTGATGAGC TCCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAACGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGTGGGAAT TTCACCTCAA AGCATGTGCC CTGGAGTTAC 
CTCGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACGGC ATTCGCCTTC 
CTGGGACTGC TCGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTTGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTCA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACAG TGGCCGACGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTCGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATCG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CAGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TCCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTGCTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGCCCGGC 
CCTGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACTGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_011248201.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011246503.1 Mus musculus two pore channel 1 (Tpcn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu12008
Clone ID Related Accession (Same CDS sequence)	XM_030254513.1 , XM_011248201.3 , NM_145853.2 , XM_011248201.2 , XM_011248201.1
Accession Version	XM_011248201.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	two pore calcium channel protein 1 isoform X1
Comment

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCGAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGGGGC 
AGCCACAGCA TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCGGAGCGGA CAGCCCCCCC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCCACAC GTTCGTCCGG CACAAACGTA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG CATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTACGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTC CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT TGCCATCCTC GGTTTCTACT TATTCTCCAC AAATCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTCGTTCT CCTGACCACA 
GCCAACTTTC CAGATGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCCGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCCTA CGGCCTGCTT GCCAGCCAAC GGAGGCCGGC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAGCCCC GGATGAGTGC AAGGGAACGC 
TTCCTGACTT TCAAGGCCTT GAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGGCAA AGAGAAACAG ACAGCATTGG 
TTTGATGAGC TCCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAACGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGTGGGAAT TTCACCTCAA AGCATGTGCC CTGGAGTTAC 
CTCGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACGGC ATTCGCCTTC 
CTGGGACTGC TCGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTTGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTCA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACAG TGGCCGACGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTCGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATCG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CAGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TCCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTGCTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGCCCGGC 
CCTGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACTGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011246503.1

Translation

MAVSLDDDVPLILTLDEAESAPLPPSNSLGQEQLPSKNGGSHSIHNSQVPSLVSGADSPPSSPTGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPVLKLHTYVHATLELFALMVVVFELCMKLRWLGFHTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFSTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYGLLASQRRPAGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKRNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFTSKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFNGRLTPNCCNTSTVADAYRFINHTVGNKTKVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQDSEVDSGIVIEKEMSKEELMAVLELYREERGTSSDVTRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQKLRGSVPGPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_011248201.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus two pore channel 1 (Tpcn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248201.1

ORF Insert Sequence:

ATGGCTGTAA GTTTAGATGA CGATGTGCCG CTCATCCTGA CCTTGGACGA GGCTGAGAGT 
GCTCCGCTGC CTCCTTCGAA CAGCCTGGGC CAAGAGCAGC TGCCCAGCAA AAATGGGGGC 
AGCCACAGCA TCCACAACTC CCAGGTCCCC AGTCTGGTCT CCGGAGCGGA CAGCCCCCCC 
TCCAGTCCCA CCGGACACAA CTGGGAGATG AATTATCAAG AGGCGGCAAT CTACCTCCAG 
GAAGGTCAGA ACAACGACAA GTTCTTCACC CACCCCAAGG ATGCCAGAGC GCTGGCGGCC 
TACCTCTTCG TCCACAACCA CTTCTTCTAC ATGATGGAGC TGCTCACGGC CCTGCTCCTG 
CTGCTGCTGT CGCTGTGCGA GTCCCCCGCT GTCCCCGTGC TCAAGCTGCA CACTTACGTC 
CACGCCACGC TGGAACTCTT TGCCCTCATG GTGGTGGTAT TTGAACTCTG CATGAAATTG 
CGGTGGCTGG GCTTCCACAC GTTCGTCCGG CACAAACGTA CCATGGTCAA GACGTCCGTC 
CTCGTGGTGC AGTTCATCGA GGCCATTGTG GTGCTGGTTC GGCAGACGTC CCACGTGCGG 
GTGACCCGGG CACTACGCTG CATTTTCCTG GTGGACTGTC GCTACTGTGG CGGTGTACGG 
CGCAACCTGC GGCAGATCTT CCAGTCTCTC CCACCCTTCA TGGACATCCT CCTGTTGCTG 
CTCTTCTTTA TGATCATCTT TGCCATCCTC GGTTTCTACT TATTCTCCAC AAATCCTTCC 
GACCCCTACT TCAGCACCCT GGAGAACAGC ATCGTCAACC TGTTCGTTCT CCTGACCACA 
GCCAACTTTC CAGATGTCAT GATGCCCTCC TACTCCCGGA ACCCCTGGTC CTGCGTCTTC 
TTCATTGTAT ACCTCTCCAT TGAGCTGTAC TTCATCATGA ACCTGCTCCT GGCCGTGGTG 
TTCGACACCT TCAACGACAT TGAAAAGCAC AAGTTCAAGT CTTTGCTGCT GCACAAACGG 
ACCGCCATCC AGCATGCCTA CGGCCTGCTT GCCAGCCAAC GGAGGCCGGC TGGCATCTCC 
TACAGGCAGT TCGAAGGCTT AATGCGCTTC TACAAGCCCC GGATGAGTGC AAGGGAACGC 
TTCCTGACTT TCAAGGCCTT GAACCAGAGC AACACGCCTC TGCTCAGCCT GAAGGACTTC 
TATGATATTT ACGAAGTCGC TGCTCTGCAG TGGAAGGCAA AGAGAAACAG ACAGCATTGG 
TTTGATGAGC TCCCCCGGAC AGCCTTCCTC ATCTTCAAAG GGATTAACAT CCTTGTGAAT 
TCCAAGGCCT TCCAGTATTT CATGTACTTG GTGGTGGCTG TCAACGGTGT CTGGATCCTG 
GTGGAGACAT TCATGTTGAA AGGTGGGAAT TTCACCTCAA AGCATGTGCC CTGGAGTTAC 
CTCGTGTTTC TTACCATCTA TGGAGTTGAA CTGTTCATGA AGGTGGCTGG CCTGGGCCCT 
GTGGAGTACC TGTCCTCTGG ATGGAACCTG TTCGATTTCT CGGTCACGGC ATTCGCCTTC 
CTGGGACTGC TCGCACTGAC GCTCAACATG GAACCCTTCT ATTTCATTGT GGTCCTGCGT 
CCCCTTCAGC TGCTGAGGTT ATTTAAACTG AAGAAACGCT ACCGCAACGT GTTGGACACC 
ATGTTTGAGC TGCTGCCGCG GATGGCCAGC CTTGGCCTCA CGCTGCTCAC CTTCTACTAT 
TCCTTCGCCA TCGTGGGCAT GGAGTTCTTC AACGGGCGGC TGACCCCCAA CTGCTGCAAC 
ACCAGCACAG TGGCCGACGC CTACCGGTTC ATCAACCACA CTGTGGGCAA TAAGACCAAG 
GTAGAGGAAG GCTACTACTA TCTCAACAAC TTTGACAACA TCCTCAACAG CTTCGTGACC 
TTGTTTGAGC TCACCGTTGT CAACAATTGG TACATCATCA TGGAAGGCGT CACCTCGCAG 
ACGTCCCACT GGAGCCGCCT GTACTTCATG ACCTTTTACA TAGTGACCAT GGTGGTGATG 
ACCATTATCG TGGCCTTCAT CCTGGAGGCC TTCGTCTTCC GCATGAACTA CAGCCGCAAG 
AGCCAGGACT CGGAAGTGGA CAGTGGCATC GTCATCGAGA AGGAAATGTC CAAGGAGGAG 
CTTATGGCCG TCCTGGAGCT TTATCGTGAG GAGCGAGGCA CCTCCTCTGA CGTGACCCGG 
CTGCTGGACA CCCTCTCTCA GATGGAGAAA TACCAGCAAA ATTCCATGGT GTTTCTGGGA 
CGGCGATCGA GAACCAAAAG TGACCTGAGT CTGAAGATGT ACCAGGAGGA GATCCAGGAG 
TGGTACGAGG AGCATGCCCG GGAACAGGAG CAGCAGAAGC TCAGGGGCAG CGTGCCCGGC 
CCTGCAGCCC AGCAGCCCCC TGGCAGTCGC CAGCGCTCCC AGACTGTCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_030254514.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_030110374.1 Mus musculus two pore channel 1 (Tpcn1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu88912
Clone ID Related Accession (Same CDS sequence)	XM_030254514.1
Accession Version	XM_030254514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	two pore calcium channel protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTAC AGAAGGGAAC CAAGACCGAA GCGTTGGGGC CCTTCATGCT CAGGGACCTG 
AGGGATGGGG GCAGCCACAG CATCCACAAC TCCCAGGTCC CCAGTCTGGT CTCCGGAGCG 
GACAGCCCCC CCTCCAGTCC CACCGGACAC AACTGGGAGA TGAATTATCA AGAGGCGGCA 
ATCTACCTCC AGGAAGGTCA GAACAACGAC AAGTTCTTCA CCCACCCCAA GGATGCCAGA 
GCGCTGGCGG CCTACCTCTT CGTCCACAAC CACTTCTTCT ACATGATGGA GCTGCTCACG 
GCCCTGCTCC TGCTGCTGCT GTCGCTGTGC GAGTCCCCCG CTGTCCCCGT GCTCAAGCTG 
CACACTTACG TCCACGCCAC GCTGGAACTC TTTGCCCTCA TGGTGGTGGT ATTTGAACTC 
TGCATGAAAT TGCGGTGGCT GGGCTTCCAC ACGTTCGTCC GGCACAAACG TACCATGGTC 
AAGACGTCCG TCCTCGTGGT GCAGTTCATC GAGGCCATTG TGGTGCTGGT TCGGCAGACG 
TCCCACGTGC GGGTGACCCG GGCACTACGC TGCATTTTCC TGGTGGACTG TCGCTACTGT 
GGCGGTGTAC GGCGCAACCT GCGGCAGATC TTCCAGTCTC TCCCACCCTT CATGGACATC 
CTCCTGTTGC TGCTCTTCTT TATGATCATC TTTGCCATCC TCGGTTTCTA CTTATTCTCC 
ACAAATCCTT CCGACCCCTA CTTCAGCACC CTGGAGAACA GCATCGTCAA CCTGTTCGTT 
CTCCTGACCA CAGCCAACTT TCCAGATGTC ATGATGCCCT CCTACTCCCG GAACCCCTGG 
TCCTGCGTCT TCTTCATTGT ATACCTCTCC ATTGAGCTGT ACTTCATCAT GAACCTGCTC 
CTGGCCGTGG TGTTCGACAC CTTCAACGAC ATTGAAAAGC ACAAGTTCAA GTCTTTGCTG 
CTGCACAAAC GGACCGCCAT CCAGCATGCC TACGGCCTGC TTGCCAGCCA ACGGAGGCCG 
GCTGGCATCT CCTACAGGCA GTTCGAAGGC TTAATGCGCT TCTACAAGCC CCGGATGAGT 
GCAAGGGAAC GCTTCCTGAC TTTCAAGGCC TTGAACCAGA GCAACACGCC TCTGCTCAGC 
CTGAAGGACT TCTATGATAT TTACGAAGTC GCTGCTCTGC AGTGGAAGGC AAAGAGAAAC 
AGACAGCATT GGTTTGATGA GCTCCCCCGG ACAGCCTTCC TCATCTTCAA AGGGATTAAC 
ATCCTTGTGA ATTCCAAGGC CTTCCAGTAT TTCATGTACT TGGTGGTGGC TGTCAACGGT 
GTCTGGATCC TGGTGGAGAC ATTCATGTTG AAAGGTGGGA ATTTCACCTC AAAGCATGTG 
CCCTGGAGTT ACCTCGTGTT TCTTACCATC TATGGAGTTG AACTGTTCAT GAAGGTGGCT 
GGCCTGGGCC CTGTGGAGTA CCTGTCCTCT GGATGGAACC TGTTCGATTT CTCGGTCACG 
GCATTCGCCT TCCTGGGACT GCTCGCACTG ACGCTCAACA TGGAACCCTT CTATTTCATT 
GTGGTCCTGC GTCCCCTTCA GCTGCTGAGG TTATTTAAAC TGAAGAAACG CTACCGCAAC 
GTGTTGGACA CCATGTTTGA GCTGCTGCCG CGGATGGCCA GCCTTGGCCT CACGCTGCTC 
ACCTTCTACT ATTCCTTCGC CATCGTGGGC ATGGAGTTCT TCAACGGGCG GCTGACCCCC 
AACTGCTGCA ACACCAGCAC AGTGGCCGAC GCCTACCGGT TCATCAACCA CACTGTGGGC 
AATAAGACCA AGGTAGAGGA AGGCTACTAC TATCTCAACA ACTTTGACAA CATCCTCAAC 
AGCTTCGTGA CCTTGTTTGA GCTCACCGTT GTCAACAATT GGTACATCAT CATGGAAGGC 
GTCACCTCGC AGACGTCCCA CTGGAGCCGC CTGTACTTCA TGACCTTTTA CATAGTGACC 
ATGGTGGTGA TGACCATTAT CGTGGCCTTC ATCCTGGAGG CCTTCGTCTT CCGCATGAAC 
TACAGCCGCA AGAGCCAGGA CTCGGAAGTG GACAGTGGCA TCGTCATCGA GAAGGAAATG 
TCCAAGGAGG AGCTTATGGC CGTCCTGGAG CTTTATCGTG AGGAGCGAGG CACCTCCTCT 
GACGTGACCC GGCTGCTGGA CACCCTCTCT CAGATGGAGA AATACCAGCA AAATTCCATG 
GTGTTTCTGG GACGGCGATC GAGAACCAAA AGTGACCTGA GTCTGAAGAT GTACCAGGAG 
GAGATCCAGG AGTGGTACGA GGAGCATGCC CGGGAACAGG AGCAGCAGAA GCTCAGGGGC 
AGCGTGCCCG GCCCTGCAGC CCAGCAGCCC CCTGGCAGTC GCCAGCGCTC CCAGACTGTC 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110374.1
CDS	38..2443
Translation	MPLQKGTKTEALGPFMLRDLRDGGSHSIHNSQVPSLVSGADSPPSSPTGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPVLKLHTYVHATLELFALMVVVFELCMKLRWLGFHTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFSTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYGLLASQRRPAGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKRNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFTSKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFNGRLTPNCCNTSTVADAYRFINHTVGNKTKVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQDSEVDSGIVIEKEMSKEELMAVLELYREERGTSSDVTRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQKLRGSVPGPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_030254514.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus two pore channel 1 (Tpcn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254514.1

ORF Insert Sequence:

ATGCCCTTAC AGAAGGGAAC CAAGACCGAA GCGTTGGGGC CCTTCATGCT CAGGGACCTG 
AGGGATGGGG GCAGCCACAG CATCCACAAC TCCCAGGTCC CCAGTCTGGT CTCCGGAGCG 
GACAGCCCCC CCTCCAGTCC CACCGGACAC AACTGGGAGA TGAATTATCA AGAGGCGGCA 
ATCTACCTCC AGGAAGGTCA GAACAACGAC AAGTTCTTCA CCCACCCCAA GGATGCCAGA 
GCGCTGGCGG CCTACCTCTT CGTCCACAAC CACTTCTTCT ACATGATGGA GCTGCTCACG 
GCCCTGCTCC TGCTGCTGCT GTCGCTGTGC GAGTCCCCCG CTGTCCCCGT GCTCAAGCTG 
CACACTTACG TCCACGCCAC GCTGGAACTC TTTGCCCTCA TGGTGGTGGT ATTTGAACTC 
TGCATGAAAT TGCGGTGGCT GGGCTTCCAC ACGTTCGTCC GGCACAAACG TACCATGGTC 
AAGACGTCCG TCCTCGTGGT GCAGTTCATC GAGGCCATTG TGGTGCTGGT TCGGCAGACG 
TCCCACGTGC GGGTGACCCG GGCACTACGC TGCATTTTCC TGGTGGACTG TCGCTACTGT 
GGCGGTGTAC GGCGCAACCT GCGGCAGATC TTCCAGTCTC TCCCACCCTT CATGGACATC 
CTCCTGTTGC TGCTCTTCTT TATGATCATC TTTGCCATCC TCGGTTTCTA CTTATTCTCC 
ACAAATCCTT CCGACCCCTA CTTCAGCACC CTGGAGAACA GCATCGTCAA CCTGTTCGTT 
CTCCTGACCA CAGCCAACTT TCCAGATGTC ATGATGCCCT CCTACTCCCG GAACCCCTGG 
TCCTGCGTCT TCTTCATTGT ATACCTCTCC ATTGAGCTGT ACTTCATCAT GAACCTGCTC 
CTGGCCGTGG TGTTCGACAC CTTCAACGAC ATTGAAAAGC ACAAGTTCAA GTCTTTGCTG 
CTGCACAAAC GGACCGCCAT CCAGCATGCC TACGGCCTGC TTGCCAGCCA ACGGAGGCCG 
GCTGGCATCT CCTACAGGCA GTTCGAAGGC TTAATGCGCT TCTACAAGCC CCGGATGAGT 
GCAAGGGAAC GCTTCCTGAC TTTCAAGGCC TTGAACCAGA GCAACACGCC TCTGCTCAGC 
CTGAAGGACT TCTATGATAT TTACGAAGTC GCTGCTCTGC AGTGGAAGGC AAAGAGAAAC 
AGACAGCATT GGTTTGATGA GCTCCCCCGG ACAGCCTTCC TCATCTTCAA AGGGATTAAC 
ATCCTTGTGA ATTCCAAGGC CTTCCAGTAT TTCATGTACT TGGTGGTGGC TGTCAACGGT 
GTCTGGATCC TGGTGGAGAC ATTCATGTTG AAAGGTGGGA ATTTCACCTC AAAGCATGTG 
CCCTGGAGTT ACCTCGTGTT TCTTACCATC TATGGAGTTG AACTGTTCAT GAAGGTGGCT 
GGCCTGGGCC CTGTGGAGTA CCTGTCCTCT GGATGGAACC TGTTCGATTT CTCGGTCACG 
GCATTCGCCT TCCTGGGACT GCTCGCACTG ACGCTCAACA TGGAACCCTT CTATTTCATT 
GTGGTCCTGC GTCCCCTTCA GCTGCTGAGG TTATTTAAAC TGAAGAAACG CTACCGCAAC 
GTGTTGGACA CCATGTTTGA GCTGCTGCCG CGGATGGCCA GCCTTGGCCT CACGCTGCTC 
ACCTTCTACT ATTCCTTCGC CATCGTGGGC ATGGAGTTCT TCAACGGGCG GCTGACCCCC 
AACTGCTGCA ACACCAGCAC AGTGGCCGAC GCCTACCGGT TCATCAACCA CACTGTGGGC 
AATAAGACCA AGGTAGAGGA AGGCTACTAC TATCTCAACA ACTTTGACAA CATCCTCAAC 
AGCTTCGTGA CCTTGTTTGA GCTCACCGTT GTCAACAATT GGTACATCAT CATGGAAGGC 
GTCACCTCGC AGACGTCCCA CTGGAGCCGC CTGTACTTCA TGACCTTTTA CATAGTGACC 
ATGGTGGTGA TGACCATTAT CGTGGCCTTC ATCCTGGAGG CCTTCGTCTT CCGCATGAAC 
TACAGCCGCA AGAGCCAGGA CTCGGAAGTG GACAGTGGCA TCGTCATCGA GAAGGAAATG 
TCCAAGGAGG AGCTTATGGC CGTCCTGGAG CTTTATCGTG AGGAGCGAGG CACCTCCTCT 
GACGTGACCC GGCTGCTGGA CACCCTCTCT CAGATGGAGA AATACCAGCA AAATTCCATG 
GTGTTTCTGG GACGGCGATC GAGAACCAAA AGTGACCTGA GTCTGAAGAT GTACCAGGAG 
GAGATCCAGG AGTGGTACGA GGAGCATGCC CGGGAACAGG AGCAGCAGAA GCTCAGGGGC 
AGCGTGCCCG GCCCTGCAGC CCAGCAGCCC CCTGGCAGTC GCCAGCGCTC CCAGACTGTC 
ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes.
Scientific reports7(1)10038(2017 Aug)
Castonguay J,Orth JHC,M?ller T,Sleman F,Grimm C,Wahl-Schott C,Biel M,Mallmann RT,Bildl W,Schulte U,Klugbauer N

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

Metabolic alterations derived from absence of Two-Pore Channel 1 at cardiac level.
Journal of biosciences41(4)643-658(2016 Dec)
Garcia-Rua V,Feijoo-Bandin S,Garcia-Vence M,Aragon-Herrera A,Bravo SB,Rodriguez-Penas D,Mosquera-Leal A,Lear PV,Parrington J,Alonso J,Rosello-Lleti E,Portoles M,Rivera M,Gonzalez-Juanatey JR,Lago F

Endolysosomal two-pore channels regulate autophagy in cardiomyocytes.
The Journal of physiology594(11)3061-77(2016 06)
Garc?a-R?a V,Feij?o-Band?n S,Rodr?guez-Penas D,Mosquera-Leal A,Abu-Assi E,Beiras A,Mar?a Seoane L,Lear P,Parrington J,Portol?s M,Rosell?-Llet? E,Rivera M,Gualillo O,Parra V,Hill JA,Rothermel B,Gonz?lez-Juanatey JR,Lago F

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TPCN1 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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