Adig cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adig
Entrez Gene ID	246747
Full Name	adipogenin
Synonyms	SMAF-1,Smaf1
General protein information	Preferred Names adipogenin Names adipogenin small adipocyte factor 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H1

mRNA and Protein(s)

mRNA	Protein	Name
NM_145635.2	NP_663610.1	adipogenin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Transcriptome Analysis of Targeted Mouse Mutations Reveals the Topography of Local Changes in Gene Expression.
West DB, Engelhard EK, Adkisson M, Nava AJ, Kirov JV, Cipollone A, Willis B, Rapp J, de Jong PJ, Lloyd KC
PLoS genetics12(2)e1005691(2016 Feb)

Expression, regulation and functional assessment of the 80 amino acid Small Adipocyte Factor 1 (Smaf1) protein in adipocytes.
Ren G, Eskandari P, Wang S, Smas CM
Archives of biochemistry and biophysics59027-36(2016 Jan)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Up-regulation of adipogenin, an adipocyte plasma transmembrane protein, during adipogenesis.
Hong YH, Hishikawa D, Miyahara H, Tsuzuki H, Nishimura Y, Gotoh C, Choi KC, Hokari Y, Takagi Y, Lee HG, Cho KK, Roh SG, Sasaki S
Molecular and cellular biochemistry276(1-2)133-41(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Together our findings show that Smaf1 is a novel small protein endogenous to adipocytes and that Smaf1 expression is closely tied to PPARgamma-mediated signals and the adipocyte phenotype.
Title: Expression, regulation and functional assessment of the 80 amino acid Small Adipocyte Factor 1 (Smaf1) protein in adipocytes.

Adipocyte-specific membrane protein adipogenin may be involved with adipogenesis as one of the regulators of adipose tissue development.
Title: Up-regulation of adipogenin, an adipocyte plasma transmembrane protein, during adipogenesis.

The following Adig gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adig cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18025	NM_145635.2 Latest version!	Mus musculus adipogenin (Adig), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adig ( NM_145635.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663610.1 Mus musculus adipogenin (Adig), mRNA.

Price & Availability↑

CloneID	OMu18025
Clone ID Related Accession (Same CDS sequence)	NM_145635.2
Accession Version	NM_145635.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	adipogenin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE624674.1, AK131610.1 and AY092026.1. On Nov 4, 2005 this sequence version replaced NM_145635.1. ##Evidence-Data-START## Transcript exon combination :: AY092026.1, BC054059.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTACC CTCTGGTGCC TCTGGTGAGC GACCTCACAC TCTCTTTCCT GGTTTTCTGG 
CTCTGCCTGC CCGTGGCTTT GCTGCTGTTC TTGACGATCG TCTGGTTGCA TTTCTTACTT 
AGTCAAGAGT CAAAGGAAGA TGATTCAGAT TTGTGCTTCA ACTGGGAGCC CTGGAGCAAA 
AGACCATCTG AGTGTGGCTG TGAGGAGACA TTTCCTGGTG AGGAGGACGG ACTCCACTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663610.1
CDS	132..374
Translation	MKYPLVPLVSDLTLSFLVFWLCLPVALLLFLTIVWLHFLLSQESKEDDSDLCFNWEPWSKRPSECGCEETFPGEEDGLHW

Target ORF information:

RefSeq Version	NM_145635.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adipogenin (Adig), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145635.2

ORF Insert Sequence:

ATGAAGTACC CTCTGGTGCC TCTGGTGAGC GACCTCACAC TCTCTTTCCT GGTTTTCTGG 
CTCTGCCTGC CCGTGGCTTT GCTGCTGTTC TTGACGATCG TCTGGTTGCA TTTCTTACTT 
AGTCAAGAGT CAAAGGAAGA TGATTCAGAT TTGTGCTTCA ACTGGGAGCC CTGGAGCAAA 
AGACCATCTG AGTGTGGCTG TGAGGAGACA TTTCCTGGTG AGGAGGACGG ACTCCACTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Transcriptome Analysis of Targeted Mouse Mutations Reveals the Topography of Local Changes in Gene Expression.
PLoS genetics12(2)e1005691(2016 Feb)
West DB,Engelhard EK,Adkisson M,Nava AJ,Kirov JV,Cipollone A,Willis B,Rapp J,de Jong PJ,Lloyd KC

Expression, regulation and functional assessment of the 80 amino acid Small Adipocyte Factor 1 (Smaf1) protein in adipocytes.
Archives of biochemistry and biophysics59027-36(2016 Jan)
Ren G,Eskandari P,Wang S,Smas CM

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Up-regulation of adipogenin, an adipocyte plasma transmembrane protein, during adipogenesis.
Molecular and cellular biochemistry276(1-2)133-41(2005 Aug)
Hong YH,Hishikawa D,Miyahara H,Tsuzuki H,Nishimura Y,Gotoh C,Choi KC,Hokari Y,Takagi Y,Lee HG,Cho KK,Roh SG,Sasaki S

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Adig cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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