Frem2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Frem2
Entrez Gene ID	242022
Full Name	Fras1 related extracellular matrix protein 2
Synonyms	6030440P17Rik,8430406N05Rik,Gm409,b2b1562Clo,my,ne,nv1
General protein information	Preferred Names Fras1 related extracellular matrix protein 2 Names FRAS1-related extracellular matrix protein 2 ECM3 homolog NV domain containing protein NV1 NV domain-containing protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 C\|3 25.24 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006501461.3	XP_006501524.1	FRAS1-related extracellular matrix protein 2 isoform X1
NM_172862.3	NP_766450.2	FRAS1-related extracellular matrix protein 2 precursor
XM_006501462.3	XP_006501525.1	FRAS1-related extracellular matrix protein 2 isoform X2
XM_006501461.4	XP_006501524.1	FRAS1-related extracellular matrix protein 2 isoform X1
XM_006501462.4	XP_006501525.1	FRAS1-related extracellular matrix protein 2 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	FREM2	NP_997244.3
Danio rerio (zebrafish)	frem2b	NP_001131133.1
Pan troglodytes (chimpanzee)	FREM2	XP_522664.2
	FREM2	XP_001086486.1
Bos taurus (cattle)	FREM2	XP_002691845.1
Canis lupus familiaris (dog)	FREM2	XP_005635476.1
Rattus norvegicus (Norway rat)	Frem2	NP_001232907.1
Mus musculus (house mouse)	Frem2	NP_766450.2
Gallus gallus (chicken)	FREM2	XP_417087.3
Danio rerio (zebrafish)	frem2a	NP_001131129.1
Xenopus tropicalis (tropical clawed frog)	LOC100496696	XP_004912033.1

Gene Ontology
Bibliography

Related articles in PubMed

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Rutledge EA, Benazet JD, McMahon AP
Development (Cambridge, England)144(17)3177-3188(2017 09)

PDGFR? regulates craniofacial development through homodimers and functional heterodimers with PDGFR?.
Fantauzzo KA, Soriano P
Genes & development30(21)2443-2458(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report Fras1 up-regulation/Frem down-regulation in nephrons from mice with polycystic kidney disease and Frem2 mutations.
Title: Expression of Fraser syndrome genes in normal and polycystic murine kidneys.

The QBRICK, together with Frem2, defines the ability of BMs to bind integrin 81 through modulating the BM assembly of nephronectin, thereby regulating the integrin 81-mediated signals transmitted from the ureteric buds to the metanephric mesenchyme.
Title: Basement membrane assembly of the integrin ?8?1 ligand nephronectin requires Fraser syndrome-associated proteins.

The localization pattern of Fras1 and Frem2 was indistinguishable, while both proteins along with Frem3 could be detected even in the absence of Frem1.
Title: Differential localization profile of Fras1/Frem proteins in epithelial basement membranes of newborn and adult mice.

Fras1 is not only essential as a component of a macromolecular complex for the extracellular stabilization of Frem2 but it is also required for its proper intracellular trafficking and export from embryonic epithelial cells.
Title: Basement membrane localization of Frem3 is independent of the Fras1/Frem1/Frem2 protein complex within the sublamina densa.

Study reports a comparative analysis of the spatiotemporal localization of Fras1, Frem1, Frem2 and Frem3 proteins during mouse embryonic development.
Title: Spatiotemporal distribution of Fras1/Frem proteins during mouse embryonic development.

The following Frem2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Frem2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu31899	XM_006501461.4 Latest version!	Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu31900	XM_006501462.4 Latest version!	Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu15006	NM_172862.3 Latest version!	Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OMu31899	XM_006501461.3	Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu31900	XM_006501462.3	Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Frem2 ( XM_006501461.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501524.1 Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), transcript variant X1, mRNA.

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CloneID	OMu31899
Clone ID Related Accession (Same CDS sequence)	XM_006501461.3 , XM_006501461.4
Accession Version	XM_006501461.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	FRAS1-related extracellular matrix protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501461.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC GTGCGCGCAG GACAGCCAAG TTCTCCAGCT TCCAGCCGAT ACTCGCACAG 
TCGCCTAGGC TGCTGCTGCT GCTGCTGCTC CTGTCACTGG TAAGCTACGT CTCCACACAG 
GCTGCTGGCC CCGGTGCAGC ACTTCAGTCC CTGGGTCTCT CCGGGACTTC GGGAGTCCCT 
ACAGAGGAAG CTATAGTGGT GGCAAACCGC GGGCTCCGTG TGCCCTTCGG TCGCGAAGTC 
TGGCTGGATC CGTTGCGTGA CTTGGTGCTG CAGGTGCAGC CAGGAGACCG CTGCACAGTG 
ACCGTGTTAG ACAACGACGC GCTGGCCCAG CGGCCGGGCC ACTTGAGTCC CAAGCGTTTT 
GCGTGCGACT ATGGCCCGGG TGAGGTGCGC TACTCGCACC TGGGTGCTCG CAGCCCTTCC 
CGCGACCGCG TCCGCCTGCA GCTGCGCTAC GATGCTCCAG GAGGGGCGAT AGTCCTGCCA 
CTGGCACTAG AGGTGGAAGT GGTCTTCACC CAGCTGGAGA TTGTGACTCG GAATTTACCT 
CTGGTCGTGG AAGAACTGCT AGGGACCAGT AATGCCTTGG ACGATCGGAG CTTAGAGTTC 
GCCTACCAGC CTGAGACCGA GGAGTGCCGC GTGGGCATCT TGTCAGGTCT GAGCGCTTTG 
CCTCGTTACG GAGAACTCCT TCACTACCCA CAGGTCCAGG GAGGGGCCGG AGATAGAGGG 
ACCTCTAAGA CCCTTTTGAT GGACTGCAAA GCATTCCAGG AGCTGGGAGT CCGCTACCGC 
CACACAGCCC CCAGCCGCTC GCCTAACAGG GACTGGCTGC CTATGGTGGT CGAGCTGCAT 
TCGCGAGGGG CTCCAGAGGG CAGCCCGGCT CTAAAACGCG AACACTTCCA GGTGCTTGTG 
AGGATTCGAG GAGGAGCGGA GAACACTGCA CCCAAGCCCA GTTTCGTGGC CATGATGATG 
ATGGAGGTGG ACCAGTTTGT ACTGACTGCT CTCACTCCAG ACATGCTAGC AGCCGAGGAT 
GCTGAATCCG ACCCAGACCT TTTGATTTTC AACCTCACCT CTGCCTTCCA GCCTGGGCAG 
GGCTACCTGG TGAGCACGGA TGACAGAAGC CTGCCTCTCT CCTCCTTTAC ACAGCGGGAT 
TTGCGCCTGC TGAAGATTGC CTACCAGCCT CCCTCTGAAG ACTCGGATCA GGAGCGTCTG 
TTTGAACTAG AGCTGGAGAT AGTGGACCCT GAAGGAGCAG CCTCAGACCC TTTCGCCTTC 
ATGGTGGTGG TGAAACCCAT GAACACACTG GCTCCCGTGG TCACACGAAA CACTGGCCTT 
ATTCTCTATG AGGGTCAATA TCGGCCGCTC ACGGGTCCCA TAGGCAGTGG ACCCCAGAAC 
TTGGTCATCA GTGATGAAGA TGACCTAGAA GCTGTGAGGC TGGAGGTGGT GGCTGGGCTC 
CGGCATGGCC ACCTTGTTAT TCTGGGTTCT CCCAGTAGCG ACTCTGCTCC CAAGACCTTC 
ACAGTGGCGG AGCTGGCGGC TGGCCAGGTT GTGTATCAGC ATGATGACAA AGACGGTTCA 
CTGAGTGACA ACTTGGTGCT GCGCATGTCC GATGGAGGAG GCAGGCACCA GGTCCAGTTC 
CTGTTCCCTA TCACTTTAGT GCCTGTGGAT GACCAGCCGC CGGTCCTGAA CGCCAACACT 
GGGCTGACAG TGGCTGAGGG GGAAACTGTG CCTATCCCAC CCTTGACTCT CAGTGCAACC 
GATATAGATT CAGATGACTC TCAGCTGGTT TTTGTCCTGT TGCCACCCTT CTCAAGCTTG 
GGGCACCTGC TTCTCCGCCA AAGGCATGTC CCCCAGGAAG AGCAGGGTCT GTGGCAGAAG 
CAAGGGTCAT TTTACGAGCG AACGGTGACA GAGTGGCGGC AACAGGATAT AACAGAGGGG 
AAGCTCTTCT ATAGGCACTC GGGGCCCCAC AGCCCTGGGC CGGTGATGGA CCAGTTCATG 
TTTAGAGTCC AAGACAATCA TGATCCCCCT AACCAGTCTG GGATCCAGAG GTTTGTAATA 
CGCATTCATC CCGTGGACCG TCTCCCTCCA GAACTGGGCA GTGGCTGTCC CCTTCGGATG 
GTGGTACAGG AATCCCAGCT CACACCACTG AGGAAAAGGT GGCTGCACTA CACTGACCTG 
GACACAGATG ACCGAGAGTT ACAGTACACA GTGACCCAGC CTCCCACGGA CACAGATGAG 
AACCACTCGC CGGCTCCACT GGGCACGTTG GTCTTCACTG ATAATCCCTC AGTGGTGGTG 
AGCCATTTTA CCCAAGCCCA GGTCAATCAT CATAAAATTG CTTACAGGCC CCCAGGGCAG 
GAGCTAGGAG TCGCAGCCCG AGTGGCTCAG TTCCAGTTCC AAGTAGAGGA TCGAGCTGGG 
AATGTGGCTC CTGGCACCTT CACCCTTTAC CTGCAGCCTG TGGATAACCA GCCCCCCGAG 
ATTGTCAACA CTGGCTTCAC CGTTGAGGAG AAGGGCCACC ACATCCTGCG TGAGACCGAG 
CTGCATGTGA GTGATGTTGA CACGGATGTG ACCCACATCT CGTTCACTCT CACACAGGCC 
CCCAAACACG GCCACATGCA AATATCTGGA CGGCCACTGC ATGTAGGCGG ACAATTCCAC 
TTGGAGGACA TAAAGCATGG CAGAATTTCC TACTGGAATA GCGGGGATGA GTCTTTGACT 
GACAGCTGCT CCCTGGAGGT CAGCGACAGA CACCATGTGG TGCCAATCAC TCTCAGAGTG 
AACGTTCGGC CAGGGGACCG TGAAGGTCCT ATGTCAGTCC TTCCTGCTGG TACTCTGGAG 
TCCTATCTAG ACGTCTTAGA GAATGGGGCT ACTGAAGTCA CTGCCAATAT CATTAAGGGG 
GCCTATCAGG GTACCGATGA TTTGATGCTG ACCTTCCTGT TAGAAGGCCC ACCCTCCTAT 
GGGGAAATCT TAGTCAACGG AGCTCCAGCT GAGCAGTTCA CCCAGAGGGA CATCCTGGAG 
GGCTCTGTTG TCTACGCTCA CACCAGTGGT GAGATTGGCC TGTTGCCCAA AGCTGACTCT 
TTTAACCTGA GTCTATCAGC TATGTCTCAA GAATGGAGAA TCGGTAGCAG CATCGTTCAA 
GGCGTCACTG TGTGGGTGAC CATCCTCCCC GTGGACAGCC AAGCCCCTGA GATTTCTCTT 
GGTGAACAGT TTGTAGTGCT GGAAGGTGAC AAGAGTGTGA TAAGCCTGAC CCACCTAAGT 
GCTGAGGACA TGGACTCCCT GAAGGATGAC CTCTTGTGCA CAATAGTCAT TCAGCCAACC 
TCAGGCTATG TAGAGAACAT TTCTCCAGCA CCAGGCTCAG AGAAATCAAG AGCCGGGGTT 
GCCATAAGTG CCTTCACGCT GAAGGATCTC AGGCAAGGAC ACATTAACTA TGTCCAGAGT 
GTCCACAGAG GAGTGGAGCC TGTCGAAGAC CGCTTTATAT TCCGTTGTTC TGACGGTATT 
AACTTCTCTG AGAGACAGAT ATTCCCCATT GTCATCATTC CTACCAATGA CGAGCAGCCA 
GAAATGTTCA TGAGGGAATT CATGGTGATG GAAGGCATGA GTCTGGTGGT CAACAGACTC 
ATCCTCAACG CTGCTGATGC CGACATTCCC AGGGATGATC TAACGTTTAC TATTACCCGG 
TTTCCAACTC ACGGTCATGT CATGAACCAG TTAATCAATG GTACAGTGTT GGTTGAAAGC 
TTCACCCTGG ATCAGATTAT AGAGAGTTCC AGCATTATTT ATGAGCACGA TGACTCTGAG 
ACACAAGAGG ACAGTTTTGT GATTAAACTG ACAGATGGCA AGCACTCCGT GGAGAAGATG 
GTCCTCATTG TGGTCATCCC TGTTGATGAC GAGACACCCA GAATGACCAT CAATAATGGG 
CTAGAAATAG AAATTGGGGA AACCAAAGTT ATCAACAACA AAGTCCTAAT GGCAACCGAT 
CTAGACTCTG ATGACAAATC ACTGGTTTAT ATTATCCGTT ATGGACCAGG GCATGGCTTG 
TTACAGAGAC AAAAACCTCT AGGTGCCTTT GAAAATATCA CTCTAGGCAT GAATTTTACC 
CAGGACGAAG TGGACAGGAA CTTGATTCAA TATGTCCACT TTGGGCAAGA AGGTATTCGA 
GACCTGATTA AATTCGATGT GACAGATGGA ACAAATGCTC TTATAGATCG CTACTTTTAC 
GTGACTATTG GAAGTGTTGA CATTGTCTTC CCTGATGTGG TAAGTAAGGG CGTGTCCTTG 
AAAGAAGGTG GCAAAGTCAC TCTGACAACG GATCTGCTGA GCACAAGTGA CTTGAACAGC 
CCTGATGAGA ACTTAGTTTT CACCATTACC AGGGCTCCTA TGAGAGGTCA CTTGGAGTGC 
ACGGATCGAA GGGGACTGTC CATCACTTCT TTCACTCAGC TCCAGCTGGC TGGTAACAAA 
ATCTACTACA TACACACGGC TGAGGATGAG GTCAAAATGG ACAGCTTCGA GTTTCAAGTC 
ACCGATGGGC GGAACCCTGT CTTCCGAACT TTCCGGATCT CAATCAGTGA CGTAGACAAC 
AAAAAGCCAG TGGTCACCAT CCACAACCTG GTGGTCAGTG AAAGTGAAAG TAAGCTGATC 
ACCCCCTTTG AACTCACTGT GGAGGACAGA GATACCCCTG ACAGGCTCCT AAAATTCATT 
GTCACCCAGG TCCCTGTTCA CGGTCACCTC CTCTTCAACA ATACCAGATC TGTCATGGTT 
TTCACCAAGC AAGACTTGAA TGAAAACTTA ATCAGCTACA AACACGATGG CACAGAATCC 
ACGGAAGACA GCTTCTCCTT CACCGTGACT GACGGCACCC ACTCGGACTT CTATGTTTTT 
CCTGATACAG TGTTTGAAAC CAGGAGACCT CAAGTGATGA AAATCCAGGT GTTACCTGTT 
GACAACAGTG TCCCCCAAAT TGTGGTGAAC AAAGGAGCTT CTACCCTTCG TACCCTGGCC 
ACTGGCCATT TAGGGTTCAT GATCACAAGC AAAATACTCA AAGTGGAGGA CAGAGACAGC 
CTGCATTTTT CTCTCAGGTT CATTGTGACA GAAGCCCCCC AACATGGCTA CCTTCTCAAC 
CTGGGCCAAG GAAACCACAG TGTCACACAG TTCACCCAAG CTGACATTGA TGACATGAAA 
ATATGCTATG TCTTGAGAGA GAGAGCAAAT GCCACAAGTG ATATGTTCCA TTTCATAGTG 
GAAGATGACG TTCTCTTTGT CCCACTCTGG CGACATTCCT TCAGACTGAG GGCAGAGGCG 
CTCGCTCGTC ACTCCTGTGC TGGCTGCTTG AGCAGAGGAG GTCATCCTGT CATCTTGGTA 
ATGCCACCTG CTCCGGCTCA CTCGGCTCAG GCCGGGGTGG GTTGTGGGGC ACACTGCAAG 
AGTGTTTCAC GGACTGTCCC TGAGAGCCAC TTCAGAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501524.1
CDS	1510..6951
Translation	MASRARRTAKFSSFQPILAQSPRLLLLLLLLSLVSYVSTQAAGPGAALQSLGLSGTSGVPTEEAIVVANRGLRVPFGREVWLDPLRDLVLQVQPGDRCTVTVLDNDALAQRPGHLSPKRFACDYGPGEVRYSHLGARSPSRDRVRLQLRYDAPGGAIVLPLALEVEVVFTQLEIVTRNLPLVVEELLGTSNALDDRSLEFAYQPETEECRVGILSGLSALPRYGELLHYPQVQGGAGDRGTSKTLLMDCKAFQELGVRYRHTAPSRSPNRDWLPMVVELHSRGAPEGSPALKREHFQVLVRIRGGAENTAPKPSFVAMMMMEVDQFVLTALTPDMLAAEDAESDPDLLIFNLTSAFQPGQGYLVSTDDRSLPLSSFTQRDLRLLKIAYQPPSEDSDQERLFELELEIVDPEGAASDPFAFMVVVKPMNTLAPVVTRNTGLILYEGQYRPLTGPIGSGPQNLVISDEDDLEAVRLEVVAGLRHGHLVILGSPSSDSAPKTFTVAELAAGQVVYQHDDKDGSLSDNLVLRMSDGGGRHQVQFLFPITLVPVDDQPPVLNANTGLTVAEGETVPIPPLTLSATDIDSDDSQLVFVLLPPFSSLGHLLLRQRHVPQEEQGLWQKQGSFYERTVTEWRQQDITEGKLFYRHSGPHSPGPVMDQFMFRVQDNHDPPNQSGIQRFVIRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKRWLHYTDLDTDDRELQYTVTQPPTDTDENHSPAPLGTLVFTDNPSVVVSHFTQAQVNHHKIAYRPPGQELGVAARVAQFQFQVEDRAGNVAPGTFTLYLQPVDNQPPEIVNTGFTVEEKGHHILRETELHVSDVDTDVTHISFTLTQAPKHGHMQISGRPLHVGGQFHLEDIKHGRISYWNSGDESLTDSCSLEVSDRHHVVPITLRVNVRPGDREGPMSVLPAGTLESYLDVLENGATEVTANIIKGAYQGTDDLMLTFLLEGPPSYGEILVNGAPAEQFTQRDILEGSVVYAHTSGEIGLLPKADSFNLSLSAMSQEWRIGSSIVQGVTVWVTILPVDSQAPEISLGEQFVVLEGDKSVISLTHLSAEDMDSLKDDLLCTIVIQPTSGYVENISPAPGSEKSRAGVAISAFTLKDLRQGHINYVQSVHRGVEPVEDRFIFRCSDGINFSERQIFPIVIIPTNDEQPEMFMREFMVMEGMSLVVNRLILNAADADIPRDDLTFTITRFPTHGHVMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGKHSVEKMVLIVVIPVDDETPRMTINNGLEIEIGETKVINNKVLMATDLDSDDKSLVYIIRYGPGHGLLQRQKPLGAFENITLGMNFTQDEVDRNLIQYVHFGQEGIRDLIKFDVTDGTNALIDRYFYVTIGSVDIVFPDVVSKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLECTDRRGLSITSFTQLQLAGNKIYYIHTAEDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHNLVVSESESKLITPFELTVEDRDTPDRLLKFIVTQVPVHGHLLFNNTRSVMVFTKQDLNENLISYKHDGTESTEDSFSFTVTDGTHSDFYVFPDTVFETRRPQVMKIQVLPVDNSVPQIVVNKGASTLRTLATGHLGFMITSKILKVEDRDSLHFSLRFIVTEAPQHGYLLNLGQGNHSVTQFTQADIDDMKICYVLRERANATSDMFHFIVEDDVLFVPLWRHSFRLRAEALARHSCAGCLSRGGHPVILVMPPAPAHSAQAGVGCGAHCKSVSRTVPESHFRN

Target ORF information:

RefSeq Version	XM_006501461.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501461.4

ORF Insert Sequence:

ATGGCCAGCC GTGCGCGCAG GACAGCCAAG TTCTCCAGCT TCCAGCCGAT ACTCGCACAG 
TCGCCTAGGC TGCTGCTGCT GCTGCTGCTC CTGTCACTGG TAAGCTACGT CTCCACACAG 
GCTGCTGGCC CCGGTGCAGC ACTTCAGTCC CTGGGTCTCT CCGGGACTTC GGGAGTCCCT 
ACAGAGGAAG CTATAGTGGT GGCAAACCGC GGGCTCCGTG TGCCCTTCGG TCGCGAAGTC 
TGGCTGGATC CGTTGCGTGA CTTGGTGCTG CAGGTGCAGC CAGGAGACCG CTGCACAGTG 
ACCGTGTTAG ACAACGACGC GCTGGCCCAG CGGCCGGGCC ACTTGAGTCC CAAGCGTTTT 
GCGTGCGACT ATGGCCCGGG TGAGGTGCGC TACTCGCACC TGGGTGCTCG CAGCCCTTCC 
CGCGACCGCG TCCGCCTGCA GCTGCGCTAC GATGCTCCAG GAGGGGCGAT AGTCCTGCCA 
CTGGCACTAG AGGTGGAAGT GGTCTTCACC CAGCTGGAGA TTGTGACTCG GAATTTACCT 
CTGGTCGTGG AAGAACTGCT AGGGACCAGT AATGCCTTGG ACGATCGGAG CTTAGAGTTC 
GCCTACCAGC CTGAGACCGA GGAGTGCCGC GTGGGCATCT TGTCAGGTCT GAGCGCTTTG 
CCTCGTTACG GAGAACTCCT TCACTACCCA CAGGTCCAGG GAGGGGCCGG AGATAGAGGG 
ACCTCTAAGA CCCTTTTGAT GGACTGCAAA GCATTCCAGG AGCTGGGAGT CCGCTACCGC 
CACACAGCCC CCAGCCGCTC GCCTAACAGG GACTGGCTGC CTATGGTGGT CGAGCTGCAT 
TCGCGAGGGG CTCCAGAGGG CAGCCCGGCT CTAAAACGCG AACACTTCCA GGTGCTTGTG 
AGGATTCGAG GAGGAGCGGA GAACACTGCA CCCAAGCCCA GTTTCGTGGC CATGATGATG 
ATGGAGGTGG ACCAGTTTGT ACTGACTGCT CTCACTCCAG ACATGCTAGC AGCCGAGGAT 
GCTGAATCCG ACCCAGACCT TTTGATTTTC AACCTCACCT CTGCCTTCCA GCCTGGGCAG 
GGCTACCTGG TGAGCACGGA TGACAGAAGC CTGCCTCTCT CCTCCTTTAC ACAGCGGGAT 
TTGCGCCTGC TGAAGATTGC CTACCAGCCT CCCTCTGAAG ACTCGGATCA GGAGCGTCTG 
TTTGAACTAG AGCTGGAGAT AGTGGACCCT GAAGGAGCAG CCTCAGACCC TTTCGCCTTC 
ATGGTGGTGG TGAAACCCAT GAACACACTG GCTCCCGTGG TCACACGAAA CACTGGCCTT 
ATTCTCTATG AGGGTCAATA TCGGCCGCTC ACGGGTCCCA TAGGCAGTGG ACCCCAGAAC 
TTGGTCATCA GTGATGAAGA TGACCTAGAA GCTGTGAGGC TGGAGGTGGT GGCTGGGCTC 
CGGCATGGCC ACCTTGTTAT TCTGGGTTCT CCCAGTAGCG ACTCTGCTCC CAAGACCTTC 
ACAGTGGCGG AGCTGGCGGC TGGCCAGGTT GTGTATCAGC ATGATGACAA AGACGGTTCA 
CTGAGTGACA ACTTGGTGCT GCGCATGTCC GATGGAGGAG GCAGGCACCA GGTCCAGTTC 
CTGTTCCCTA TCACTTTAGT GCCTGTGGAT GACCAGCCGC CGGTCCTGAA CGCCAACACT 
GGGCTGACAG TGGCTGAGGG GGAAACTGTG CCTATCCCAC CCTTGACTCT CAGTGCAACC 
GATATAGATT CAGATGACTC TCAGCTGGTT TTTGTCCTGT TGCCACCCTT CTCAAGCTTG 
GGGCACCTGC TTCTCCGCCA AAGGCATGTC CCCCAGGAAG AGCAGGGTCT GTGGCAGAAG 
CAAGGGTCAT TTTACGAGCG AACGGTGACA GAGTGGCGGC AACAGGATAT AACAGAGGGG 
AAGCTCTTCT ATAGGCACTC GGGGCCCCAC AGCCCTGGGC CGGTGATGGA CCAGTTCATG 
TTTAGAGTCC AAGACAATCA TGATCCCCCT AACCAGTCTG GGATCCAGAG GTTTGTAATA 
CGCATTCATC CCGTGGACCG TCTCCCTCCA GAACTGGGCA GTGGCTGTCC CCTTCGGATG 
GTGGTACAGG AATCCCAGCT CACACCACTG AGGAAAAGGT GGCTGCACTA CACTGACCTG 
GACACAGATG ACCGAGAGTT ACAGTACACA GTGACCCAGC CTCCCACGGA CACAGATGAG 
AACCACTCGC CGGCTCCACT GGGCACGTTG GTCTTCACTG ATAATCCCTC AGTGGTGGTG 
AGCCATTTTA CCCAAGCCCA GGTCAATCAT CATAAAATTG CTTACAGGCC CCCAGGGCAG 
GAGCTAGGAG TCGCAGCCCG AGTGGCTCAG TTCCAGTTCC AAGTAGAGGA TCGAGCTGGG 
AATGTGGCTC CTGGCACCTT CACCCTTTAC CTGCAGCCTG TGGATAACCA GCCCCCCGAG 
ATTGTCAACA CTGGCTTCAC CGTTGAGGAG AAGGGCCACC ACATCCTGCG TGAGACCGAG 
CTGCATGTGA GTGATGTTGA CACGGATGTG ACCCACATCT CGTTCACTCT CACACAGGCC 
CCCAAACACG GCCACATGCA AATATCTGGA CGGCCACTGC ATGTAGGCGG ACAATTCCAC 
TTGGAGGACA TAAAGCATGG CAGAATTTCC TACTGGAATA GCGGGGATGA GTCTTTGACT 
GACAGCTGCT CCCTGGAGGT CAGCGACAGA CACCATGTGG TGCCAATCAC TCTCAGAGTG 
AACGTTCGGC CAGGGGACCG TGAAGGTCCT ATGTCAGTCC TTCCTGCTGG TACTCTGGAG 
TCCTATCTAG ACGTCTTAGA GAATGGGGCT ACTGAAGTCA CTGCCAATAT CATTAAGGGG 
GCCTATCAGG GTACCGATGA TTTGATGCTG ACCTTCCTGT TAGAAGGCCC ACCCTCCTAT 
GGGGAAATCT TAGTCAACGG AGCTCCAGCT GAGCAGTTCA CCCAGAGGGA CATCCTGGAG 
GGCTCTGTTG TCTACGCTCA CACCAGTGGT GAGATTGGCC TGTTGCCCAA AGCTGACTCT 
TTTAACCTGA GTCTATCAGC TATGTCTCAA GAATGGAGAA TCGGTAGCAG CATCGTTCAA 
GGCGTCACTG TGTGGGTGAC CATCCTCCCC GTGGACAGCC AAGCCCCTGA GATTTCTCTT 
GGTGAACAGT TTGTAGTGCT GGAAGGTGAC AAGAGTGTGA TAAGCCTGAC CCACCTAAGT 
GCTGAGGACA TGGACTCCCT GAAGGATGAC CTCTTGTGCA CAATAGTCAT TCAGCCAACC 
TCAGGCTATG TAGAGAACAT TTCTCCAGCA CCAGGCTCAG AGAAATCAAG AGCCGGGGTT 
GCCATAAGTG CCTTCACGCT GAAGGATCTC AGGCAAGGAC ACATTAACTA TGTCCAGAGT 
GTCCACAGAG GAGTGGAGCC TGTCGAAGAC CGCTTTATAT TCCGTTGTTC TGACGGTATT 
AACTTCTCTG AGAGACAGAT ATTCCCCATT GTCATCATTC CTACCAATGA CGAGCAGCCA 
GAAATGTTCA TGAGGGAATT CATGGTGATG GAAGGCATGA GTCTGGTGGT CAACAGACTC 
ATCCTCAACG CTGCTGATGC CGACATTCCC AGGGATGATC TAACGTTTAC TATTACCCGG 
TTTCCAACTC ACGGTCATGT CATGAACCAG TTAATCAATG GTACAGTGTT GGTTGAAAGC 
TTCACCCTGG ATCAGATTAT AGAGAGTTCC AGCATTATTT ATGAGCACGA TGACTCTGAG 
ACACAAGAGG ACAGTTTTGT GATTAAACTG ACAGATGGCA AGCACTCCGT GGAGAAGATG 
GTCCTCATTG TGGTCATCCC TGTTGATGAC GAGACACCCA GAATGACCAT CAATAATGGG 
CTAGAAATAG AAATTGGGGA AACCAAAGTT ATCAACAACA AAGTCCTAAT GGCAACCGAT 
CTAGACTCTG ATGACAAATC ACTGGTTTAT ATTATCCGTT ATGGACCAGG GCATGGCTTG 
TTACAGAGAC AAAAACCTCT AGGTGCCTTT GAAAATATCA CTCTAGGCAT GAATTTTACC 
CAGGACGAAG TGGACAGGAA CTTGATTCAA TATGTCCACT TTGGGCAAGA AGGTATTCGA 
GACCTGATTA AATTCGATGT GACAGATGGA ACAAATGCTC TTATAGATCG CTACTTTTAC 
GTGACTATTG GAAGTGTTGA CATTGTCTTC CCTGATGTGG TAAGTAAGGG CGTGTCCTTG 
AAAGAAGGTG GCAAAGTCAC TCTGACAACG GATCTGCTGA GCACAAGTGA CTTGAACAGC 
CCTGATGAGA ACTTAGTTTT CACCATTACC AGGGCTCCTA TGAGAGGTCA CTTGGAGTGC 
ACGGATCGAA GGGGACTGTC CATCACTTCT TTCACTCAGC TCCAGCTGGC TGGTAACAAA 
ATCTACTACA TACACACGGC TGAGGATGAG GTCAAAATGG ACAGCTTCGA GTTTCAAGTC 
ACCGATGGGC GGAACCCTGT CTTCCGAACT TTCCGGATCT CAATCAGTGA CGTAGACAAC 
AAAAAGCCAG TGGTCACCAT CCACAACCTG GTGGTCAGTG AAAGTGAAAG TAAGCTGATC 
ACCCCCTTTG AACTCACTGT GGAGGACAGA GATACCCCTG ACAGGCTCCT AAAATTCATT 
GTCACCCAGG TCCCTGTTCA CGGTCACCTC CTCTTCAACA ATACCAGATC TGTCATGGTT 
TTCACCAAGC AAGACTTGAA TGAAAACTTA ATCAGCTACA AACACGATGG CACAGAATCC 
ACGGAAGACA GCTTCTCCTT CACCGTGACT GACGGCACCC ACTCGGACTT CTATGTTTTT 
CCTGATACAG TGTTTGAAAC CAGGAGACCT CAAGTGATGA AAATCCAGGT GTTACCTGTT 
GACAACAGTG TCCCCCAAAT TGTGGTGAAC AAAGGAGCTT CTACCCTTCG TACCCTGGCC 
ACTGGCCATT TAGGGTTCAT GATCACAAGC AAAATACTCA AAGTGGAGGA CAGAGACAGC 
CTGCATTTTT CTCTCAGGTT CATTGTGACA GAAGCCCCCC AACATGGCTA CCTTCTCAAC 
CTGGGCCAAG GAAACCACAG TGTCACACAG TTCACCCAAG CTGACATTGA TGACATGAAA 
ATATGCTATG TCTTGAGAGA GAGAGCAAAT GCCACAAGTG ATATGTTCCA TTTCATAGTG 
GAAGATGACG TTCTCTTTGT CCCACTCTGG CGACATTCCT TCAGACTGAG GGCAGAGGCG 
CTCGCTCGTC ACTCCTGTGC TGGCTGCTTG AGCAGAGGAG GTCATCCTGT CATCTTGGTA 
ATGCCACCTG CTCCGGCTCA CTCGGCTCAG GCCGGGGTGG GTTGTGGGGC ACACTGCAAG 
AGTGTTTCAC GGACTGTCCC TGAGAGCCAC TTCAGAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frem2 ( XM_006501462.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501525.1 Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), transcript variant X2, mRNA.

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CloneID	OMu31900
Clone ID Related Accession (Same CDS sequence)	XM_006501462.3 , XM_006501462.4
Accession Version	XM_006501462.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	FRAS1-related extracellular matrix protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501462.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC GTGCGCGCAG GACAGCCAAG TTCTCCAGCT TCCAGCCGAT ACTCGCACAG 
TCGCCTAGGC TGCTGCTGCT GCTGCTGCTC CTGTCACTGG TAAGCTACGT CTCCACACAG 
GCTGCTGGCC CCGGTGCAGC ACTTCAGTCC CTGGGTCTCT CCGGGACTTC GGGAGTCCCT 
ACAGAGGAAG CTATAGTGGT GGCAAACCGC GGGCTCCGTG TGCCCTTCGG TCGCGAAGTC 
TGGCTGGATC CGTTGCGTGA CTTGGTGCTG CAGGTGCAGC CAGGAGACCG CTGCACAGTG 
ACCGTGTTAG ACAACGACGC GCTGGCCCAG CGGCCGGGCC ACTTGAGTCC CAAGCGTTTT 
GCGTGCGACT ATGGCCCGGG TGAGGTGCGC TACTCGCACC TGGGTGCTCG CAGCCCTTCC 
CGCGACCGCG TCCGCCTGCA GCTGCGCTAC GATGCTCCAG GAGGGGCGAT AGTCCTGCCA 
CTGGCACTAG AGGTGGAAGT GGTCTTCACC CAGCTGGAGA TTGTGACTCG GAATTTACCT 
CTGGTCGTGG AAGAACTGCT AGGGACCAGT AATGCCTTGG ACGATCGGAG CTTAGAGTTC 
GCCTACCAGC CTGAGACCGA GGAGTGCCGC GTGGGCATCT TGTCAGGTCT GAGCGCTTTG 
CCTCGTTACG GAGAACTCCT TCACTACCCA CAGGTCCAGG GAGGGGCCGG AGATAGAGGG 
ACCTCTAAGA CCCTTTTGAT GGACTGCAAA GCATTCCAGG AGCTGGGAGT CCGCTACCGC 
CACACAGCCC CCAGCCGCTC GCCTAACAGG GACTGGCTGC CTATGGTGGT CGAGCTGCAT 
TCGCGAGGGG CTCCAGAGGG CAGCCCGGCT CTAAAACGCG AACACTTCCA GGTGCTTGTG 
AGGATTCGAG GAGGAGCGGA GAACACTGCA CCCAAGCCCA GTTTCGTGGC CATGATGATG 
ATGGAGGTGG ACCAGTTTGT ACTGACTGCT CTCACTCCAG ACATGCTAGC AGCCGAGGAT 
GCTGAATCCG ACCCAGACCT TTTGATTTTC AACCTCACCT CTGCCTTCCA GCCTGGGCAG 
GGCTACCTGG TGAGCACGGA TGACAGAAGC CTGCCTCTCT CCTCCTTTAC ACAGCGGGAT 
TTGCGCCTGC TGAAGATTGC CTACCAGCCT CCCTCTGAAG ACTCGGATCA GGAGCGTCTG 
TTTGAACTAG AGCTGGAGAT AGTGGACCCT GAAGGAGCAG CCTCAGACCC TTTCGCCTTC 
ATGGTGGTGG TGAAACCCAT GAACACACTG GCTCCCGTGG TCACACGAAA CACTGGCCTT 
ATTCTCTATG AGGGTCAATA TCGGCCGCTC ACGGGTCCCA TAGGCAGTGG ACCCCAGAAC 
TTGGTCATCA GTGATGAAGA TGACCTAGAA GCTGTGAGGC TGGAGGTGGT GGCTGGGCTC 
CGGCATGGCC ACCTTGTTAT TCTGGGTTCT CCCAGTAGCG ACTCTGCTCC CAAGACCTTC 
ACAGTGGCGG AGCTGGCGGC TGGCCAGGTT GTGTATCAGC ATGATGACAA AGACGGTTCA 
CTGAGTGACA ACTTGGTGCT GCGCATGTCC GATGGAGGAG GCAGGCACCA GGTCCAGTTC 
CTGTTCCCTA TCACTTTAGT GCCTGTGGAT GACCAGCCGC CGGTCCTGAA CGCCAACACT 
GGGCTGACAG TGGCTGAGGG GGAAACTGTG CCTATCCCAC CCTTGACTCT CAGTGCAACC 
GATATAGATT CAGATGACTC TCAGCTGGTT TTTGTCCTGT TGCCACCCTT CTCAAGCTTG 
GGGCACCTGC TTCTCCGCCA AAGGCATGTC CCCCAGGAAG AGCAGGGTCT GTGGCAGAAG 
CAAGGGTCAT TTTACGAGCG AACGGTGACA GAGTGGCGGC AACAGGATAT AACAGAGGGG 
AAGCTCTTCT ATAGGCACTC GGGGCCCCAC AGCCCTGGGC CGGTGATGGA CCAGTTCATG 
TTTAGAGTCC AAGACAATCA TGATCCCCCT AACCAGTCTG GGATCCAGAG GTTTGTAATA 
CGCATTCATC CCGTGGACCG TCTCCCTCCA GAACTGGGCA GTGGCTGTCC CCTTCGGATG 
GTGGTACAGG AATCCCAGCT CACACCACTG AGGAAAAGGT GGCTGCACTA CACTGACCTG 
GACACAGATG ACCGAGAGTT ACAGTACACA GTGACCCAGC CTCCCACGGA CACAGATGAG 
AACCACTCGC CGGCTCCACT GGGCACGTTG GTCTTCACTG ATAATCCCTC AGTGGTGGTG 
AGCCATTTTA CCCAAGCCCA GGTCAATCAT CATAAAATTG CTTACAGGCC CCCAGGGCAG 
GAGCTAGGAG TCGCAGCCCG AGTGGCTCAG TTCCAGTTCC AAGTAGAGGA TCGAGCTGGG 
AATGTGGCTC CTGGCACCTT CACCCTTTAC CTGCAGCCTG TGGATAACCA GCCCCCCGAG 
ATTGTCAACA CTGGCTTCAC CGTTGAGGAG AAGGGCCACC ACATCCTGCG TGAGACCGAG 
CTGCATGTGA GTGATGTTGA CACGGATGTG ACCCACATCT CGTTCACTCT CACACAGGCC 
CCCAAACACG GCCACATGCA AATATCTGGA CGGCCACTGC ATGTAGGCGG ACAATTCCAC 
TTGGAGGACA TAAAGCATGG CAGAATTTCC TACTGGAATA GCGGGGATGA GTCTTTGACT 
GACAGCTGCT CCCTGGAGGT CAGCGACAGA CACCATGTGG TGCCAATCAC TCTCAGAGTG 
AACGTTCGGC CAGGGGACCG TGAAGGTCCT ATGTCAGTCC TTCCTGCTGG TACTCTGGAG 
TCCTATCTAG ACGTCTTAGA GAATGGGGCT ACTGAAGTCA CTGCCAATAT CATTAAGGGG 
GCCTATCAGG GTACCGATGA TTTGATGCTG ACCTTCCTGT TAGAAGGCCC ACCCTCCTAT 
GGGGAAATCT TAGTCAACGG AGCTCCAGCT GAGCAGTTCA CCCAGAGGGA CATCCTGGAG 
GGCTCTGTTG TCTACGCTCA CACCAGTGGT GAGATTGGCC TGTTGCCCAA AGCTGACTCT 
TTTAACCTGA GTCTATCAGC TATGTCTCAA GAATGGAGAA TCGGTAGCAG CATCGTTCAA 
GGCGTCACTG TGTGGGTGAC CATCCTCCCC GTGGACAGCC AAGCCCCTGA GATTTCTCTT 
GGTGAACAGT TTGTAGTGCT GGAAGGTGAC AAGAGTGTGA TAAGCCTGAC CCACCTAAGT 
GCTGAGGACA TGGACTCCCT GAAGGATGAC CTCTTGTGCA CAATAGTCAT TCAGCCAACC 
TCAGGCTATG TAGAGAACAT TTCTCCAGCA CCAGGCTCAG AGAAATCAAG AGCCGGGGTT 
GCCATAAGTG CCTTCACGCT GAAGGATCTC AGGCAAGGAC ACATTAACTA TGTCCAGAGT 
GTCCACAGAG GAGTGGAGCC TGTCGAAGAC CGCTTTATAT TCCGTTGTTC TGACGGTATT 
AACTTCTCTG AGAGACAGAT ATTCCCCATT GTCATCATTC CTACCAATGA CGAGCAGCCA 
GAAATGTTCA TGAGGGAATT CATGGTGATG GAAGGCATGA GTCTGGTGGT CAACAGACTC 
ATCCTCAACG CTGCTGATGC CGACATTCCC AGGGATGATC TAACGTTTAC TATTACCCGG 
TTTCCAACTC ACGGTCATGT CATGAACCAG TTAATCAATG GTACAGTGTT GGTTGAAAGC 
TTCACCCTGG ATCAGATTAT AGAGAGTTCC AGCATTATTT ATGAGCACGA TGACTCTGAG 
ACACAAGAGG ACAGTTTTGT GATTAAACTG ACAGATGGCA AGCACTCCGT GGAGAAGATG 
GTCCTCATTG TGGTCATCCC TGTTGATGAC GAGACACCCA GAATGACCAT CAATAATGGG 
CTAGAAATAG AAATTGGGGA AACCAAAGTT ATCAACAACA AAGTCCTAAT GGCAACCGAT 
CTAGACTCTG ATGACAAATC ACTGGTTTAT ATTATCCGTT ATGGACCAGG GCATGGCTTG 
TTACAGAGAC AAAAACCTCT AGGTGCCTTT GAAAATATCA CTCTAGGCAT GAATTTTACC 
CAGGACGAAG TGGACAGGAA CTTGATTCAA TATGTCCACT TTGGGCAAGA AGGTATTCGA 
GACCTGATTA AATTCGATGT GACAGATGGA ACAAATGCTC TTATAGATCG CTACTTTTAC 
GTGACTATTG GAAGTGTTGA CATTGTCTTC CCTGATGTGG TAAGTAAGGG CGTGTCCTTG 
AAAGAAGGTG GCAAAGTCAC TCTGACAACG GATCTGCTGA GCACAAGTGA CTTGAACAGC 
CCTGATGAGA ACTTAGTTTT CACCATTACC AGGGCTCCTA TGAGAGGTCA CTTGGAGTGC 
ACGGATCGAA GGGGACTGTC CATCACTTCT TTCACTCAGC TCCAGCTGGC TGGTAACAAA 
ATCTACTACA TACACACGGC TGAGGATGAG GTCAAAATGG ACAGCTTCGA GTTTCAAGTC 
ACCGATGGGC GGAACCCTGT CTTCCGAACT TTCCGGATCT CAATCAGTGA CGTAGACAAC 
AAAAAGCCAG TGGTCACCAT CCACAACCTG GTGGTCAGTG AAAGTGAAAG TAAGCTGATC 
ACCCCCTTTG AACTCACTGT GGAGGACAGA GATACCCCTG ACAGGCTCCT AAAATTCATT 
GTCACCCAGG TCCCTGTTCA CGGTCACCTC CTCTTCAACA ATACCAGATC TGTCATGGTT 
TTCACCAAGC AAGACTTGAA TGAAAACTTA ATCAGCTACA AACACGATGG CACAGAATCC 
ACGGAAGACA GCTTCTCCTT CACCGTGACT GACGGCACCC ACTCGGACTT CTATGTTTTT 
CCTGATACAG TGTTTGAAAC CAGGAGACCT CAAGTGATGA AAATCCAGGT GTTACCTGTT 
GACAACAGTG TCCCCCAAAT TGTGGTGAAC AAAGGAGCTT CTACCCTTCG TACCCTGGCC 
ACTGGCCATT TAGGGTTCAT GATCACAAGC AAAATACTCA AAGTGGAGGA CAGAGACAGC 
CTGCATTTTT CTCTCAGGTT CATTGTGACA GAAGCCCCCC AACATGGCTA CCTTCTCAAC 
CTGGGCCAAG GAAACCACAG TGTCACACAG TTCACCCAAG CTGACATTGA TGACATGAAA 
ATATGCTATG TCTTGAGAGA GAGAGCAAAT GCCACAAGTG ATATGTTCCA TTTCATAGTG 
GAAGATGACG ATGAAAGATC CCATCCGCTG TGCAAGGGGA AGGGCTGTGT TTCTAACAGA 
GGCTGGACCC TGGCACCCAG GAGGCTCTCA CTGAGTAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501525.1
CDS	1510..6831
Translation	MASRARRTAKFSSFQPILAQSPRLLLLLLLLSLVSYVSTQAAGPGAALQSLGLSGTSGVPTEEAIVVANRGLRVPFGREVWLDPLRDLVLQVQPGDRCTVTVLDNDALAQRPGHLSPKRFACDYGPGEVRYSHLGARSPSRDRVRLQLRYDAPGGAIVLPLALEVEVVFTQLEIVTRNLPLVVEELLGTSNALDDRSLEFAYQPETEECRVGILSGLSALPRYGELLHYPQVQGGAGDRGTSKTLLMDCKAFQELGVRYRHTAPSRSPNRDWLPMVVELHSRGAPEGSPALKREHFQVLVRIRGGAENTAPKPSFVAMMMMEVDQFVLTALTPDMLAAEDAESDPDLLIFNLTSAFQPGQGYLVSTDDRSLPLSSFTQRDLRLLKIAYQPPSEDSDQERLFELELEIVDPEGAASDPFAFMVVVKPMNTLAPVVTRNTGLILYEGQYRPLTGPIGSGPQNLVISDEDDLEAVRLEVVAGLRHGHLVILGSPSSDSAPKTFTVAELAAGQVVYQHDDKDGSLSDNLVLRMSDGGGRHQVQFLFPITLVPVDDQPPVLNANTGLTVAEGETVPIPPLTLSATDIDSDDSQLVFVLLPPFSSLGHLLLRQRHVPQEEQGLWQKQGSFYERTVTEWRQQDITEGKLFYRHSGPHSPGPVMDQFMFRVQDNHDPPNQSGIQRFVIRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKRWLHYTDLDTDDRELQYTVTQPPTDTDENHSPAPLGTLVFTDNPSVVVSHFTQAQVNHHKIAYRPPGQELGVAARVAQFQFQVEDRAGNVAPGTFTLYLQPVDNQPPEIVNTGFTVEEKGHHILRETELHVSDVDTDVTHISFTLTQAPKHGHMQISGRPLHVGGQFHLEDIKHGRISYWNSGDESLTDSCSLEVSDRHHVVPITLRVNVRPGDREGPMSVLPAGTLESYLDVLENGATEVTANIIKGAYQGTDDLMLTFLLEGPPSYGEILVNGAPAEQFTQRDILEGSVVYAHTSGEIGLLPKADSFNLSLSAMSQEWRIGSSIVQGVTVWVTILPVDSQAPEISLGEQFVVLEGDKSVISLTHLSAEDMDSLKDDLLCTIVIQPTSGYVENISPAPGSEKSRAGVAISAFTLKDLRQGHINYVQSVHRGVEPVEDRFIFRCSDGINFSERQIFPIVIIPTNDEQPEMFMREFMVMEGMSLVVNRLILNAADADIPRDDLTFTITRFPTHGHVMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGKHSVEKMVLIVVIPVDDETPRMTINNGLEIEIGETKVINNKVLMATDLDSDDKSLVYIIRYGPGHGLLQRQKPLGAFENITLGMNFTQDEVDRNLIQYVHFGQEGIRDLIKFDVTDGTNALIDRYFYVTIGSVDIVFPDVVSKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLECTDRRGLSITSFTQLQLAGNKIYYIHTAEDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHNLVVSESESKLITPFELTVEDRDTPDRLLKFIVTQVPVHGHLLFNNTRSVMVFTKQDLNENLISYKHDGTESTEDSFSFTVTDGTHSDFYVFPDTVFETRRPQVMKIQVLPVDNSVPQIVVNKGASTLRTLATGHLGFMITSKILKVEDRDSLHFSLRFIVTEAPQHGYLLNLGQGNHSVTQFTQADIDDMKICYVLRERANATSDMFHFIVEDDDERSHPLCKGKGCVSNRGWTLAPRRLSLSS

Target ORF information:

RefSeq Version	XM_006501462.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501462.4

ORF Insert Sequence:

ATGGCCAGCC GTGCGCGCAG GACAGCCAAG TTCTCCAGCT TCCAGCCGAT ACTCGCACAG 
TCGCCTAGGC TGCTGCTGCT GCTGCTGCTC CTGTCACTGG TAAGCTACGT CTCCACACAG 
GCTGCTGGCC CCGGTGCAGC ACTTCAGTCC CTGGGTCTCT CCGGGACTTC GGGAGTCCCT 
ACAGAGGAAG CTATAGTGGT GGCAAACCGC GGGCTCCGTG TGCCCTTCGG TCGCGAAGTC 
TGGCTGGATC CGTTGCGTGA CTTGGTGCTG CAGGTGCAGC CAGGAGACCG CTGCACAGTG 
ACCGTGTTAG ACAACGACGC GCTGGCCCAG CGGCCGGGCC ACTTGAGTCC CAAGCGTTTT 
GCGTGCGACT ATGGCCCGGG TGAGGTGCGC TACTCGCACC TGGGTGCTCG CAGCCCTTCC 
CGCGACCGCG TCCGCCTGCA GCTGCGCTAC GATGCTCCAG GAGGGGCGAT AGTCCTGCCA 
CTGGCACTAG AGGTGGAAGT GGTCTTCACC CAGCTGGAGA TTGTGACTCG GAATTTACCT 
CTGGTCGTGG AAGAACTGCT AGGGACCAGT AATGCCTTGG ACGATCGGAG CTTAGAGTTC 
GCCTACCAGC CTGAGACCGA GGAGTGCCGC GTGGGCATCT TGTCAGGTCT GAGCGCTTTG 
CCTCGTTACG GAGAACTCCT TCACTACCCA CAGGTCCAGG GAGGGGCCGG AGATAGAGGG 
ACCTCTAAGA CCCTTTTGAT GGACTGCAAA GCATTCCAGG AGCTGGGAGT CCGCTACCGC 
CACACAGCCC CCAGCCGCTC GCCTAACAGG GACTGGCTGC CTATGGTGGT CGAGCTGCAT 
TCGCGAGGGG CTCCAGAGGG CAGCCCGGCT CTAAAACGCG AACACTTCCA GGTGCTTGTG 
AGGATTCGAG GAGGAGCGGA GAACACTGCA CCCAAGCCCA GTTTCGTGGC CATGATGATG 
ATGGAGGTGG ACCAGTTTGT ACTGACTGCT CTCACTCCAG ACATGCTAGC AGCCGAGGAT 
GCTGAATCCG ACCCAGACCT TTTGATTTTC AACCTCACCT CTGCCTTCCA GCCTGGGCAG 
GGCTACCTGG TGAGCACGGA TGACAGAAGC CTGCCTCTCT CCTCCTTTAC ACAGCGGGAT 
TTGCGCCTGC TGAAGATTGC CTACCAGCCT CCCTCTGAAG ACTCGGATCA GGAGCGTCTG 
TTTGAACTAG AGCTGGAGAT AGTGGACCCT GAAGGAGCAG CCTCAGACCC TTTCGCCTTC 
ATGGTGGTGG TGAAACCCAT GAACACACTG GCTCCCGTGG TCACACGAAA CACTGGCCTT 
ATTCTCTATG AGGGTCAATA TCGGCCGCTC ACGGGTCCCA TAGGCAGTGG ACCCCAGAAC 
TTGGTCATCA GTGATGAAGA TGACCTAGAA GCTGTGAGGC TGGAGGTGGT GGCTGGGCTC 
CGGCATGGCC ACCTTGTTAT TCTGGGTTCT CCCAGTAGCG ACTCTGCTCC CAAGACCTTC 
ACAGTGGCGG AGCTGGCGGC TGGCCAGGTT GTGTATCAGC ATGATGACAA AGACGGTTCA 
CTGAGTGACA ACTTGGTGCT GCGCATGTCC GATGGAGGAG GCAGGCACCA GGTCCAGTTC 
CTGTTCCCTA TCACTTTAGT GCCTGTGGAT GACCAGCCGC CGGTCCTGAA CGCCAACACT 
GGGCTGACAG TGGCTGAGGG GGAAACTGTG CCTATCCCAC CCTTGACTCT CAGTGCAACC 
GATATAGATT CAGATGACTC TCAGCTGGTT TTTGTCCTGT TGCCACCCTT CTCAAGCTTG 
GGGCACCTGC TTCTCCGCCA AAGGCATGTC CCCCAGGAAG AGCAGGGTCT GTGGCAGAAG 
CAAGGGTCAT TTTACGAGCG AACGGTGACA GAGTGGCGGC AACAGGATAT AACAGAGGGG 
AAGCTCTTCT ATAGGCACTC GGGGCCCCAC AGCCCTGGGC CGGTGATGGA CCAGTTCATG 
TTTAGAGTCC AAGACAATCA TGATCCCCCT AACCAGTCTG GGATCCAGAG GTTTGTAATA 
CGCATTCATC CCGTGGACCG TCTCCCTCCA GAACTGGGCA GTGGCTGTCC CCTTCGGATG 
GTGGTACAGG AATCCCAGCT CACACCACTG AGGAAAAGGT GGCTGCACTA CACTGACCTG 
GACACAGATG ACCGAGAGTT ACAGTACACA GTGACCCAGC CTCCCACGGA CACAGATGAG 
AACCACTCGC CGGCTCCACT GGGCACGTTG GTCTTCACTG ATAATCCCTC AGTGGTGGTG 
AGCCATTTTA CCCAAGCCCA GGTCAATCAT CATAAAATTG CTTACAGGCC CCCAGGGCAG 
GAGCTAGGAG TCGCAGCCCG AGTGGCTCAG TTCCAGTTCC AAGTAGAGGA TCGAGCTGGG 
AATGTGGCTC CTGGCACCTT CACCCTTTAC CTGCAGCCTG TGGATAACCA GCCCCCCGAG 
ATTGTCAACA CTGGCTTCAC CGTTGAGGAG AAGGGCCACC ACATCCTGCG TGAGACCGAG 
CTGCATGTGA GTGATGTTGA CACGGATGTG ACCCACATCT CGTTCACTCT CACACAGGCC 
CCCAAACACG GCCACATGCA AATATCTGGA CGGCCACTGC ATGTAGGCGG ACAATTCCAC 
TTGGAGGACA TAAAGCATGG CAGAATTTCC TACTGGAATA GCGGGGATGA GTCTTTGACT 
GACAGCTGCT CCCTGGAGGT CAGCGACAGA CACCATGTGG TGCCAATCAC TCTCAGAGTG 
AACGTTCGGC CAGGGGACCG TGAAGGTCCT ATGTCAGTCC TTCCTGCTGG TACTCTGGAG 
TCCTATCTAG ACGTCTTAGA GAATGGGGCT ACTGAAGTCA CTGCCAATAT CATTAAGGGG 
GCCTATCAGG GTACCGATGA TTTGATGCTG ACCTTCCTGT TAGAAGGCCC ACCCTCCTAT 
GGGGAAATCT TAGTCAACGG AGCTCCAGCT GAGCAGTTCA CCCAGAGGGA CATCCTGGAG 
GGCTCTGTTG TCTACGCTCA CACCAGTGGT GAGATTGGCC TGTTGCCCAA AGCTGACTCT 
TTTAACCTGA GTCTATCAGC TATGTCTCAA GAATGGAGAA TCGGTAGCAG CATCGTTCAA 
GGCGTCACTG TGTGGGTGAC CATCCTCCCC GTGGACAGCC AAGCCCCTGA GATTTCTCTT 
GGTGAACAGT TTGTAGTGCT GGAAGGTGAC AAGAGTGTGA TAAGCCTGAC CCACCTAAGT 
GCTGAGGACA TGGACTCCCT GAAGGATGAC CTCTTGTGCA CAATAGTCAT TCAGCCAACC 
TCAGGCTATG TAGAGAACAT TTCTCCAGCA CCAGGCTCAG AGAAATCAAG AGCCGGGGTT 
GCCATAAGTG CCTTCACGCT GAAGGATCTC AGGCAAGGAC ACATTAACTA TGTCCAGAGT 
GTCCACAGAG GAGTGGAGCC TGTCGAAGAC CGCTTTATAT TCCGTTGTTC TGACGGTATT 
AACTTCTCTG AGAGACAGAT ATTCCCCATT GTCATCATTC CTACCAATGA CGAGCAGCCA 
GAAATGTTCA TGAGGGAATT CATGGTGATG GAAGGCATGA GTCTGGTGGT CAACAGACTC 
ATCCTCAACG CTGCTGATGC CGACATTCCC AGGGATGATC TAACGTTTAC TATTACCCGG 
TTTCCAACTC ACGGTCATGT CATGAACCAG TTAATCAATG GTACAGTGTT GGTTGAAAGC 
TTCACCCTGG ATCAGATTAT AGAGAGTTCC AGCATTATTT ATGAGCACGA TGACTCTGAG 
ACACAAGAGG ACAGTTTTGT GATTAAACTG ACAGATGGCA AGCACTCCGT GGAGAAGATG 
GTCCTCATTG TGGTCATCCC TGTTGATGAC GAGACACCCA GAATGACCAT CAATAATGGG 
CTAGAAATAG AAATTGGGGA AACCAAAGTT ATCAACAACA AAGTCCTAAT GGCAACCGAT 
CTAGACTCTG ATGACAAATC ACTGGTTTAT ATTATCCGTT ATGGACCAGG GCATGGCTTG 
TTACAGAGAC AAAAACCTCT AGGTGCCTTT GAAAATATCA CTCTAGGCAT GAATTTTACC 
CAGGACGAAG TGGACAGGAA CTTGATTCAA TATGTCCACT TTGGGCAAGA AGGTATTCGA 
GACCTGATTA AATTCGATGT GACAGATGGA ACAAATGCTC TTATAGATCG CTACTTTTAC 
GTGACTATTG GAAGTGTTGA CATTGTCTTC CCTGATGTGG TAAGTAAGGG CGTGTCCTTG 
AAAGAAGGTG GCAAAGTCAC TCTGACAACG GATCTGCTGA GCACAAGTGA CTTGAACAGC 
CCTGATGAGA ACTTAGTTTT CACCATTACC AGGGCTCCTA TGAGAGGTCA CTTGGAGTGC 
ACGGATCGAA GGGGACTGTC CATCACTTCT TTCACTCAGC TCCAGCTGGC TGGTAACAAA 
ATCTACTACA TACACACGGC TGAGGATGAG GTCAAAATGG ACAGCTTCGA GTTTCAAGTC 
ACCGATGGGC GGAACCCTGT CTTCCGAACT TTCCGGATCT CAATCAGTGA CGTAGACAAC 
AAAAAGCCAG TGGTCACCAT CCACAACCTG GTGGTCAGTG AAAGTGAAAG TAAGCTGATC 
ACCCCCTTTG AACTCACTGT GGAGGACAGA GATACCCCTG ACAGGCTCCT AAAATTCATT 
GTCACCCAGG TCCCTGTTCA CGGTCACCTC CTCTTCAACA ATACCAGATC TGTCATGGTT 
TTCACCAAGC AAGACTTGAA TGAAAACTTA ATCAGCTACA AACACGATGG CACAGAATCC 
ACGGAAGACA GCTTCTCCTT CACCGTGACT GACGGCACCC ACTCGGACTT CTATGTTTTT 
CCTGATACAG TGTTTGAAAC CAGGAGACCT CAAGTGATGA AAATCCAGGT GTTACCTGTT 
GACAACAGTG TCCCCCAAAT TGTGGTGAAC AAAGGAGCTT CTACCCTTCG TACCCTGGCC 
ACTGGCCATT TAGGGTTCAT GATCACAAGC AAAATACTCA AAGTGGAGGA CAGAGACAGC 
CTGCATTTTT CTCTCAGGTT CATTGTGACA GAAGCCCCCC AACATGGCTA CCTTCTCAAC 
CTGGGCCAAG GAAACCACAG TGTCACACAG TTCACCCAAG CTGACATTGA TGACATGAAA 
ATATGCTATG TCTTGAGAGA GAGAGCAAAT GCCACAAGTG ATATGTTCCA TTTCATAGTG 
GAAGATGACG ATGAAAGATC CCATCCGCTG TGCAAGGGGA AGGGCTGTGT TTCTAACAGA 
GGCTGGACCC TGGCACCCAG GAGGCTCTCA CTGAGTAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frem2 ( XM_006501461.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501524.1 Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), transcript variant X1, mRNA.

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CloneID	OMu31899
Clone ID Related Accession (Same CDS sequence)	XM_006501461.3 , XM_006501461.4
Accession Version	XM_006501461.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	FRAS1-related extracellular matrix protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501461.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC GTGCGCGCAG GACAGCCAAG TTCTCCAGCT TCCAGCCGAT ACTCGCACAG 
TCGCCTAGGC TGCTGCTGCT GCTGCTGCTC CTGTCACTGG TAAGCTACGT CTCCACACAG 
GCTGCTGGCC CCGGTGCAGC ACTTCAGTCC CTGGGTCTCT CCGGGACTTC GGGAGTCCCT 
ACAGAGGAAG CTATAGTGGT GGCAAACCGC GGGCTCCGTG TGCCCTTCGG TCGCGAAGTC 
TGGCTGGATC CGTTGCGTGA CTTGGTGCTG CAGGTGCAGC CAGGAGACCG CTGCACAGTG 
ACCGTGTTAG ACAACGACGC GCTGGCCCAG CGGCCGGGCC ACTTGAGTCC CAAGCGTTTT 
GCGTGCGACT ATGGCCCGGG TGAGGTGCGC TACTCGCACC TGGGTGCTCG CAGCCCTTCC 
CGCGACCGCG TCCGCCTGCA GCTGCGCTAC GATGCTCCAG GAGGGGCGAT AGTCCTGCCA 
CTGGCACTAG AGGTGGAAGT GGTCTTCACC CAGCTGGAGA TTGTGACTCG GAATTTACCT 
CTGGTCGTGG AAGAACTGCT AGGGACCAGT AATGCCTTGG ACGATCGGAG CTTAGAGTTC 
GCCTACCAGC CTGAGACCGA GGAGTGCCGC GTGGGCATCT TGTCAGGTCT GAGCGCTTTG 
CCTCGTTACG GAGAACTCCT TCACTACCCA CAGGTCCAGG GAGGGGCCGG AGATAGAGGG 
ACCTCTAAGA CCCTTTTGAT GGACTGCAAA GCATTCCAGG AGCTGGGAGT CCGCTACCGC 
CACACAGCCC CCAGCCGCTC GCCTAACAGG GACTGGCTGC CTATGGTGGT CGAGCTGCAT 
TCGCGAGGGG CTCCAGAGGG CAGCCCGGCT CTAAAACGCG AACACTTCCA GGTGCTTGTG 
AGGATTCGAG GAGGAGCGGA GAACACTGCA CCCAAGCCCA GTTTCGTGGC CATGATGATG 
ATGGAGGTGG ACCAGTTTGT ACTGACTGCT CTCACTCCAG ACATGCTAGC AGCCGAGGAT 
GCTGAATCCG ACCCAGACCT TTTGATTTTC AACCTCACCT CTGCCTTCCA GCCTGGGCAG 
GGCTACCTGG TGAGCACGGA TGACAGAAGC CTGCCTCTCT CCTCCTTTAC ACAGCGGGAT 
TTGCGCCTGC TGAAGATTGC CTACCAGCCT CCCTCTGAAG ACTCGGATCA GGAGCGTCTG 
TTTGAACTAG AGCTGGAGAT AGTGGACCCT GAAGGAGCAG CCTCAGACCC TTTCGCCTTC 
ATGGTGGTGG TGAAACCCAT GAACACACTG GCTCCCGTGG TCACACGAAA CACTGGCCTT 
ATTCTCTATG AGGGTCAATA TCGGCCGCTC ACGGGTCCCA TAGGCAGTGG ACCCCAGAAC 
TTGGTCATCA GTGATGAAGA TGACCTAGAA GCTGTGAGGC TGGAGGTGGT GGCTGGGCTC 
CGGCATGGCC ACCTTGTTAT TCTGGGTTCT CCCAGTAGCG ACTCTGCTCC CAAGACCTTC 
ACAGTGGCGG AGCTGGCGGC TGGCCAGGTT GTGTATCAGC ATGATGACAA AGACGGTTCA 
CTGAGTGACA ACTTGGTGCT GCGCATGTCC GATGGAGGAG GCAGGCACCA GGTCCAGTTC 
CTGTTCCCTA TCACTTTAGT GCCTGTGGAT GACCAGCCGC CGGTCCTGAA CGCCAACACT 
GGGCTGACAG TGGCTGAGGG GGAAACTGTG CCTATCCCAC CCTTGACTCT CAGTGCAACC 
GATATAGATT CAGATGACTC TCAGCTGGTT TTTGTCCTGT TGCCACCCTT CTCAAGCTTG 
GGGCACCTGC TTCTCCGCCA AAGGCATGTC CCCCAGGAAG AGCAGGGTCT GTGGCAGAAG 
CAAGGGTCAT TTTACGAGCG AACGGTGACA GAGTGGCGGC AACAGGATAT AACAGAGGGG 
AAGCTCTTCT ATAGGCACTC GGGGCCCCAC AGCCCTGGGC CGGTGATGGA CCAGTTCATG 
TTTAGAGTCC AAGACAATCA TGATCCCCCT AACCAGTCTG GGATCCAGAG GTTTGTAATA 
CGCATTCATC CCGTGGACCG TCTCCCTCCA GAACTGGGCA GTGGCTGTCC CCTTCGGATG 
GTGGTACAGG AATCCCAGCT CACACCACTG AGGAAAAGGT GGCTGCACTA CACTGACCTG 
GACACAGATG ACCGAGAGTT ACAGTACACA GTGACCCAGC CTCCCACGGA CACAGATGAG 
AACCACTCGC CGGCTCCACT GGGCACGTTG GTCTTCACTG ATAATCCCTC AGTGGTGGTG 
AGCCATTTTA CCCAAGCCCA GGTCAATCAT CATAAAATTG CTTACAGGCC CCCAGGGCAG 
GAGCTAGGAG TCGCAGCCCG AGTGGCTCAG TTCCAGTTCC AAGTAGAGGA TCGAGCTGGG 
AATGTGGCTC CTGGCACCTT CACCCTTTAC CTGCAGCCTG TGGATAACCA GCCCCCCGAG 
ATTGTCAACA CTGGCTTCAC CGTTGAGGAG AAGGGCCACC ACATCCTGCG TGAGACCGAG 
CTGCATGTGA GTGATGTTGA CACGGATGTG ACCCACATCT CGTTCACTCT CACACAGGCC 
CCCAAACACG GCCACATGCA AATATCTGGA CGGCCACTGC ATGTAGGCGG ACAATTCCAC 
TTGGAGGACA TAAAGCATGG CAGAATTTCC TACTGGAATA GCGGGGATGA GTCTTTGACT 
GACAGCTGCT CCCTGGAGGT CAGCGACAGA CACCATGTGG TGCCAATCAC TCTCAGAGTG 
AACGTTCGGC CAGGGGACCG TGAAGGTCCT ATGTCAGTCC TTCCTGCTGG TACTCTGGAG 
TCCTATCTAG ACGTCTTAGA GAATGGGGCT ACTGAAGTCA CTGCCAATAT CATTAAGGGG 
GCCTATCAGG GTACCGATGA TTTGATGCTG ACCTTCCTGT TAGAAGGCCC ACCCTCCTAT 
GGGGAAATCT TAGTCAACGG AGCTCCAGCT GAGCAGTTCA CCCAGAGGGA CATCCTGGAG 
GGCTCTGTTG TCTACGCTCA CACCAGTGGT GAGATTGGCC TGTTGCCCAA AGCTGACTCT 
TTTAACCTGA GTCTATCAGC TATGTCTCAA GAATGGAGAA TCGGTAGCAG CATCGTTCAA 
GGCGTCACTG TGTGGGTGAC CATCCTCCCC GTGGACAGCC AAGCCCCTGA GATTTCTCTT 
GGTGAACAGT TTGTAGTGCT GGAAGGTGAC AAGAGTGTGA TAAGCCTGAC CCACCTAAGT 
GCTGAGGACA TGGACTCCCT GAAGGATGAC CTCTTGTGCA CAATAGTCAT TCAGCCAACC 
TCAGGCTATG TAGAGAACAT TTCTCCAGCA CCAGGCTCAG AGAAATCAAG AGCCGGGGTT 
GCCATAAGTG CCTTCACGCT GAAGGATCTC AGGCAAGGAC ACATTAACTA TGTCCAGAGT 
GTCCACAGAG GAGTGGAGCC TGTCGAAGAC CGCTTTATAT TCCGTTGTTC TGACGGTATT 
AACTTCTCTG AGAGACAGAT ATTCCCCATT GTCATCATTC CTACCAATGA CGAGCAGCCA 
GAAATGTTCA TGAGGGAATT CATGGTGATG GAAGGCATGA GTCTGGTGGT CAACAGACTC 
ATCCTCAACG CTGCTGATGC CGACATTCCC AGGGATGATC TAACGTTTAC TATTACCCGG 
TTTCCAACTC ACGGTCATGT CATGAACCAG TTAATCAATG GTACAGTGTT GGTTGAAAGC 
TTCACCCTGG ATCAGATTAT AGAGAGTTCC AGCATTATTT ATGAGCACGA TGACTCTGAG 
ACACAAGAGG ACAGTTTTGT GATTAAACTG ACAGATGGCA AGCACTCCGT GGAGAAGATG 
GTCCTCATTG TGGTCATCCC TGTTGATGAC GAGACACCCA GAATGACCAT CAATAATGGG 
CTAGAAATAG AAATTGGGGA AACCAAAGTT ATCAACAACA AAGTCCTAAT GGCAACCGAT 
CTAGACTCTG ATGACAAATC ACTGGTTTAT ATTATCCGTT ATGGACCAGG GCATGGCTTG 
TTACAGAGAC AAAAACCTCT AGGTGCCTTT GAAAATATCA CTCTAGGCAT GAATTTTACC 
CAGGACGAAG TGGACAGGAA CTTGATTCAA TATGTCCACT TTGGGCAAGA AGGTATTCGA 
GACCTGATTA AATTCGATGT GACAGATGGA ACAAATGCTC TTATAGATCG CTACTTTTAC 
GTGACTATTG GAAGTGTTGA CATTGTCTTC CCTGATGTGG TAAGTAAGGG CGTGTCCTTG 
AAAGAAGGTG GCAAAGTCAC TCTGACAACG GATCTGCTGA GCACAAGTGA CTTGAACAGC 
CCTGATGAGA ACTTAGTTTT CACCATTACC AGGGCTCCTA TGAGAGGTCA CTTGGAGTGC 
ACGGATCGAA GGGGACTGTC CATCACTTCT TTCACTCAGC TCCAGCTGGC TGGTAACAAA 
ATCTACTACA TACACACGGC TGAGGATGAG GTCAAAATGG ACAGCTTCGA GTTTCAAGTC 
ACCGATGGGC GGAACCCTGT CTTCCGAACT TTCCGGATCT CAATCAGTGA CGTAGACAAC 
AAAAAGCCAG TGGTCACCAT CCACAACCTG GTGGTCAGTG AAAGTGAAAG TAAGCTGATC 
ACCCCCTTTG AACTCACTGT GGAGGACAGA GATACCCCTG ACAGGCTCCT AAAATTCATT 
GTCACCCAGG TCCCTGTTCA CGGTCACCTC CTCTTCAACA ATACCAGATC TGTCATGGTT 
TTCACCAAGC AAGACTTGAA TGAAAACTTA ATCAGCTACA AACACGATGG CACAGAATCC 
ACGGAAGACA GCTTCTCCTT CACCGTGACT GACGGCACCC ACTCGGACTT CTATGTTTTT 
CCTGATACAG TGTTTGAAAC CAGGAGACCT CAAGTGATGA AAATCCAGGT GTTACCTGTT 
GACAACAGTG TCCCCCAAAT TGTGGTGAAC AAAGGAGCTT CTACCCTTCG TACCCTGGCC 
ACTGGCCATT TAGGGTTCAT GATCACAAGC AAAATACTCA AAGTGGAGGA CAGAGACAGC 
CTGCATTTTT CTCTCAGGTT CATTGTGACA GAAGCCCCCC AACATGGCTA CCTTCTCAAC 
CTGGGCCAAG GAAACCACAG TGTCACACAG TTCACCCAAG CTGACATTGA TGACATGAAA 
ATATGCTATG TCTTGAGAGA GAGAGCAAAT GCCACAAGTG ATATGTTCCA TTTCATAGTG 
GAAGATGACG TTCTCTTTGT CCCACTCTGG CGACATTCCT TCAGACTGAG GGCAGAGGCG 
CTCGCTCGTC ACTCCTGTGC TGGCTGCTTG AGCAGAGGAG GTCATCCTGT CATCTTGGTA 
ATGCCACCTG CTCCGGCTCA CTCGGCTCAG GCCGGGGTGG GTTGTGGGGC ACACTGCAAG 
AGTGTTTCAC GGACTGTCCC TGAGAGCCAC TTCAGAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501524.1
CDS	100..5541
Translation	MASRARRTAKFSSFQPILAQSPRLLLLLLLLSLVSYVSTQAAGPGAALQSLGLSGTSGVPTEEAIVVANRGLRVPFGREVWLDPLRDLVLQVQPGDRCTVTVLDNDALAQRPGHLSPKRFACDYGPGEVRYSHLGARSPSRDRVRLQLRYDAPGGAIVLPLALEVEVVFTQLEIVTRNLPLVVEELLGTSNALDDRSLEFAYQPETEECRVGILSGLSALPRYGELLHYPQVQGGAGDRGTSKTLLMDCKAFQELGVRYRHTAPSRSPNRDWLPMVVELHSRGAPEGSPALKREHFQVLVRIRGGAENTAPKPSFVAMMMMEVDQFVLTALTPDMLAAEDAESDPDLLIFNLTSAFQPGQGYLVSTDDRSLPLSSFTQRDLRLLKIAYQPPSEDSDQERLFELELEIVDPEGAASDPFAFMVVVKPMNTLAPVVTRNTGLILYEGQYRPLTGPIGSGPQNLVISDEDDLEAVRLEVVAGLRHGHLVILGSPSSDSAPKTFTVAELAAGQVVYQHDDKDGSLSDNLVLRMSDGGGRHQVQFLFPITLVPVDDQPPVLNANTGLTVAEGETVPIPPLTLSATDIDSDDSQLVFVLLPPFSSLGHLLLRQRHVPQEEQGLWQKQGSFYERTVTEWRQQDITEGKLFYRHSGPHSPGPVMDQFMFRVQDNHDPPNQSGIQRFVIRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKRWLHYTDLDTDDRELQYTVTQPPTDTDENHSPAPLGTLVFTDNPSVVVSHFTQAQVNHHKIAYRPPGQELGVAARVAQFQFQVEDRAGNVAPGTFTLYLQPVDNQPPEIVNTGFTVEEKGHHILRETELHVSDVDTDVTHISFTLTQAPKHGHMQISGRPLHVGGQFHLEDIKHGRISYWNSGDESLTDSCSLEVSDRHHVVPITLRVNVRPGDREGPMSVLPAGTLESYLDVLENGATEVTANIIKGAYQGTDDLMLTFLLEGPPSYGEILVNGAPAEQFTQRDILEGSVVYAHTSGEIGLLPKADSFNLSLSAMSQEWRIGSSIVQGVTVWVTILPVDSQAPEISLGEQFVVLEGDKSVISLTHLSAEDMDSLKDDLLCTIVIQPTSGYVENISPAPGSEKSRAGVAISAFTLKDLRQGHINYVQSVHRGVEPVEDRFIFRCSDGINFSERQIFPIVIIPTNDEQPEMFMREFMVMEGMSLVVNRLILNAADADIPRDDLTFTITRFPTHGHVMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGKHSVEKMVLIVVIPVDDETPRMTINNGLEIEIGETKVINNKVLMATDLDSDDKSLVYIIRYGPGHGLLQRQKPLGAFENITLGMNFTQDEVDRNLIQYVHFGQEGIRDLIKFDVTDGTNALIDRYFYVTIGSVDIVFPDVVSKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLECTDRRGLSITSFTQLQLAGNKIYYIHTAEDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHNLVVSESESKLITPFELTVEDRDTPDRLLKFIVTQVPVHGHLLFNNTRSVMVFTKQDLNENLISYKHDGTESTEDSFSFTVTDGTHSDFYVFPDTVFETRRPQVMKIQVLPVDNSVPQIVVNKGASTLRTLATGHLGFMITSKILKVEDRDSLHFSLRFIVTEAPQHGYLLNLGQGNHSVTQFTQADIDDMKICYVLRERANATSDMFHFIVEDDVLFVPLWRHSFRLRAEALARHSCAGCLSRGGHPVILVMPPAPAHSAQAGVGCGAHCKSVSRTVPESHFRN

Target ORF information:

RefSeq Version	XM_006501461.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501461.3

ORF Insert Sequence:

ATGGCCAGCC GTGCGCGCAG GACAGCCAAG TTCTCCAGCT TCCAGCCGAT ACTCGCACAG 
TCGCCTAGGC TGCTGCTGCT GCTGCTGCTC CTGTCACTGG TAAGCTACGT CTCCACACAG 
GCTGCTGGCC CCGGTGCAGC ACTTCAGTCC CTGGGTCTCT CCGGGACTTC GGGAGTCCCT 
ACAGAGGAAG CTATAGTGGT GGCAAACCGC GGGCTCCGTG TGCCCTTCGG TCGCGAAGTC 
TGGCTGGATC CGTTGCGTGA CTTGGTGCTG CAGGTGCAGC CAGGAGACCG CTGCACAGTG 
ACCGTGTTAG ACAACGACGC GCTGGCCCAG CGGCCGGGCC ACTTGAGTCC CAAGCGTTTT 
GCGTGCGACT ATGGCCCGGG TGAGGTGCGC TACTCGCACC TGGGTGCTCG CAGCCCTTCC 
CGCGACCGCG TCCGCCTGCA GCTGCGCTAC GATGCTCCAG GAGGGGCGAT AGTCCTGCCA 
CTGGCACTAG AGGTGGAAGT GGTCTTCACC CAGCTGGAGA TTGTGACTCG GAATTTACCT 
CTGGTCGTGG AAGAACTGCT AGGGACCAGT AATGCCTTGG ACGATCGGAG CTTAGAGTTC 
GCCTACCAGC CTGAGACCGA GGAGTGCCGC GTGGGCATCT TGTCAGGTCT GAGCGCTTTG 
CCTCGTTACG GAGAACTCCT TCACTACCCA CAGGTCCAGG GAGGGGCCGG AGATAGAGGG 
ACCTCTAAGA CCCTTTTGAT GGACTGCAAA GCATTCCAGG AGCTGGGAGT CCGCTACCGC 
CACACAGCCC CCAGCCGCTC GCCTAACAGG GACTGGCTGC CTATGGTGGT CGAGCTGCAT 
TCGCGAGGGG CTCCAGAGGG CAGCCCGGCT CTAAAACGCG AACACTTCCA GGTGCTTGTG 
AGGATTCGAG GAGGAGCGGA GAACACTGCA CCCAAGCCCA GTTTCGTGGC CATGATGATG 
ATGGAGGTGG ACCAGTTTGT ACTGACTGCT CTCACTCCAG ACATGCTAGC AGCCGAGGAT 
GCTGAATCCG ACCCAGACCT TTTGATTTTC AACCTCACCT CTGCCTTCCA GCCTGGGCAG 
GGCTACCTGG TGAGCACGGA TGACAGAAGC CTGCCTCTCT CCTCCTTTAC ACAGCGGGAT 
TTGCGCCTGC TGAAGATTGC CTACCAGCCT CCCTCTGAAG ACTCGGATCA GGAGCGTCTG 
TTTGAACTAG AGCTGGAGAT AGTGGACCCT GAAGGAGCAG CCTCAGACCC TTTCGCCTTC 
ATGGTGGTGG TGAAACCCAT GAACACACTG GCTCCCGTGG TCACACGAAA CACTGGCCTT 
ATTCTCTATG AGGGTCAATA TCGGCCGCTC ACGGGTCCCA TAGGCAGTGG ACCCCAGAAC 
TTGGTCATCA GTGATGAAGA TGACCTAGAA GCTGTGAGGC TGGAGGTGGT GGCTGGGCTC 
CGGCATGGCC ACCTTGTTAT TCTGGGTTCT CCCAGTAGCG ACTCTGCTCC CAAGACCTTC 
ACAGTGGCGG AGCTGGCGGC TGGCCAGGTT GTGTATCAGC ATGATGACAA AGACGGTTCA 
CTGAGTGACA ACTTGGTGCT GCGCATGTCC GATGGAGGAG GCAGGCACCA GGTCCAGTTC 
CTGTTCCCTA TCACTTTAGT GCCTGTGGAT GACCAGCCGC CGGTCCTGAA CGCCAACACT 
GGGCTGACAG TGGCTGAGGG GGAAACTGTG CCTATCCCAC CCTTGACTCT CAGTGCAACC 
GATATAGATT CAGATGACTC TCAGCTGGTT TTTGTCCTGT TGCCACCCTT CTCAAGCTTG 
GGGCACCTGC TTCTCCGCCA AAGGCATGTC CCCCAGGAAG AGCAGGGTCT GTGGCAGAAG 
CAAGGGTCAT TTTACGAGCG AACGGTGACA GAGTGGCGGC AACAGGATAT AACAGAGGGG 
AAGCTCTTCT ATAGGCACTC GGGGCCCCAC AGCCCTGGGC CGGTGATGGA CCAGTTCATG 
TTTAGAGTCC AAGACAATCA TGATCCCCCT AACCAGTCTG GGATCCAGAG GTTTGTAATA 
CGCATTCATC CCGTGGACCG TCTCCCTCCA GAACTGGGCA GTGGCTGTCC CCTTCGGATG 
GTGGTACAGG AATCCCAGCT CACACCACTG AGGAAAAGGT GGCTGCACTA CACTGACCTG 
GACACAGATG ACCGAGAGTT ACAGTACACA GTGACCCAGC CTCCCACGGA CACAGATGAG 
AACCACTCGC CGGCTCCACT GGGCACGTTG GTCTTCACTG ATAATCCCTC AGTGGTGGTG 
AGCCATTTTA CCCAAGCCCA GGTCAATCAT CATAAAATTG CTTACAGGCC CCCAGGGCAG 
GAGCTAGGAG TCGCAGCCCG AGTGGCTCAG TTCCAGTTCC AAGTAGAGGA TCGAGCTGGG 
AATGTGGCTC CTGGCACCTT CACCCTTTAC CTGCAGCCTG TGGATAACCA GCCCCCCGAG 
ATTGTCAACA CTGGCTTCAC CGTTGAGGAG AAGGGCCACC ACATCCTGCG TGAGACCGAG 
CTGCATGTGA GTGATGTTGA CACGGATGTG ACCCACATCT CGTTCACTCT CACACAGGCC 
CCCAAACACG GCCACATGCA AATATCTGGA CGGCCACTGC ATGTAGGCGG ACAATTCCAC 
TTGGAGGACA TAAAGCATGG CAGAATTTCC TACTGGAATA GCGGGGATGA GTCTTTGACT 
GACAGCTGCT CCCTGGAGGT CAGCGACAGA CACCATGTGG TGCCAATCAC TCTCAGAGTG 
AACGTTCGGC CAGGGGACCG TGAAGGTCCT ATGTCAGTCC TTCCTGCTGG TACTCTGGAG 
TCCTATCTAG ACGTCTTAGA GAATGGGGCT ACTGAAGTCA CTGCCAATAT CATTAAGGGG 
GCCTATCAGG GTACCGATGA TTTGATGCTG ACCTTCCTGT TAGAAGGCCC ACCCTCCTAT 
GGGGAAATCT TAGTCAACGG AGCTCCAGCT GAGCAGTTCA CCCAGAGGGA CATCCTGGAG 
GGCTCTGTTG TCTACGCTCA CACCAGTGGT GAGATTGGCC TGTTGCCCAA AGCTGACTCT 
TTTAACCTGA GTCTATCAGC TATGTCTCAA GAATGGAGAA TCGGTAGCAG CATCGTTCAA 
GGCGTCACTG TGTGGGTGAC CATCCTCCCC GTGGACAGCC AAGCCCCTGA GATTTCTCTT 
GGTGAACAGT TTGTAGTGCT GGAAGGTGAC AAGAGTGTGA TAAGCCTGAC CCACCTAAGT 
GCTGAGGACA TGGACTCCCT GAAGGATGAC CTCTTGTGCA CAATAGTCAT TCAGCCAACC 
TCAGGCTATG TAGAGAACAT TTCTCCAGCA CCAGGCTCAG AGAAATCAAG AGCCGGGGTT 
GCCATAAGTG CCTTCACGCT GAAGGATCTC AGGCAAGGAC ACATTAACTA TGTCCAGAGT 
GTCCACAGAG GAGTGGAGCC TGTCGAAGAC CGCTTTATAT TCCGTTGTTC TGACGGTATT 
AACTTCTCTG AGAGACAGAT ATTCCCCATT GTCATCATTC CTACCAATGA CGAGCAGCCA 
GAAATGTTCA TGAGGGAATT CATGGTGATG GAAGGCATGA GTCTGGTGGT CAACAGACTC 
ATCCTCAACG CTGCTGATGC CGACATTCCC AGGGATGATC TAACGTTTAC TATTACCCGG 
TTTCCAACTC ACGGTCATGT CATGAACCAG TTAATCAATG GTACAGTGTT GGTTGAAAGC 
TTCACCCTGG ATCAGATTAT AGAGAGTTCC AGCATTATTT ATGAGCACGA TGACTCTGAG 
ACACAAGAGG ACAGTTTTGT GATTAAACTG ACAGATGGCA AGCACTCCGT GGAGAAGATG 
GTCCTCATTG TGGTCATCCC TGTTGATGAC GAGACACCCA GAATGACCAT CAATAATGGG 
CTAGAAATAG AAATTGGGGA AACCAAAGTT ATCAACAACA AAGTCCTAAT GGCAACCGAT 
CTAGACTCTG ATGACAAATC ACTGGTTTAT ATTATCCGTT ATGGACCAGG GCATGGCTTG 
TTACAGAGAC AAAAACCTCT AGGTGCCTTT GAAAATATCA CTCTAGGCAT GAATTTTACC 
CAGGACGAAG TGGACAGGAA CTTGATTCAA TATGTCCACT TTGGGCAAGA AGGTATTCGA 
GACCTGATTA AATTCGATGT GACAGATGGA ACAAATGCTC TTATAGATCG CTACTTTTAC 
GTGACTATTG GAAGTGTTGA CATTGTCTTC CCTGATGTGG TAAGTAAGGG CGTGTCCTTG 
AAAGAAGGTG GCAAAGTCAC TCTGACAACG GATCTGCTGA GCACAAGTGA CTTGAACAGC 
CCTGATGAGA ACTTAGTTTT CACCATTACC AGGGCTCCTA TGAGAGGTCA CTTGGAGTGC 
ACGGATCGAA GGGGACTGTC CATCACTTCT TTCACTCAGC TCCAGCTGGC TGGTAACAAA 
ATCTACTACA TACACACGGC TGAGGATGAG GTCAAAATGG ACAGCTTCGA GTTTCAAGTC 
ACCGATGGGC GGAACCCTGT CTTCCGAACT TTCCGGATCT CAATCAGTGA CGTAGACAAC 
AAAAAGCCAG TGGTCACCAT CCACAACCTG GTGGTCAGTG AAAGTGAAAG TAAGCTGATC 
ACCCCCTTTG AACTCACTGT GGAGGACAGA GATACCCCTG ACAGGCTCCT AAAATTCATT 
GTCACCCAGG TCCCTGTTCA CGGTCACCTC CTCTTCAACA ATACCAGATC TGTCATGGTT 
TTCACCAAGC AAGACTTGAA TGAAAACTTA ATCAGCTACA AACACGATGG CACAGAATCC 
ACGGAAGACA GCTTCTCCTT CACCGTGACT GACGGCACCC ACTCGGACTT CTATGTTTTT 
CCTGATACAG TGTTTGAAAC CAGGAGACCT CAAGTGATGA AAATCCAGGT GTTACCTGTT 
GACAACAGTG TCCCCCAAAT TGTGGTGAAC AAAGGAGCTT CTACCCTTCG TACCCTGGCC 
ACTGGCCATT TAGGGTTCAT GATCACAAGC AAAATACTCA AAGTGGAGGA CAGAGACAGC 
CTGCATTTTT CTCTCAGGTT CATTGTGACA GAAGCCCCCC AACATGGCTA CCTTCTCAAC 
CTGGGCCAAG GAAACCACAG TGTCACACAG TTCACCCAAG CTGACATTGA TGACATGAAA 
ATATGCTATG TCTTGAGAGA GAGAGCAAAT GCCACAAGTG ATATGTTCCA TTTCATAGTG 
GAAGATGACG TTCTCTTTGT CCCACTCTGG CGACATTCCT TCAGACTGAG GGCAGAGGCG 
CTCGCTCGTC ACTCCTGTGC TGGCTGCTTG AGCAGAGGAG GTCATCCTGT CATCTTGGTA 
ATGCCACCTG CTCCGGCTCA CTCGGCTCAG GCCGGGGTGG GTTGTGGGGC ACACTGCAAG 
AGTGTTTCAC GGACTGTCCC TGAGAGCCAC TTCAGAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frem2 ( XM_006501462.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501525.1 Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), transcript variant X2, mRNA.

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CloneID	OMu31900
Clone ID Related Accession (Same CDS sequence)	XM_006501462.3 , XM_006501462.4
Accession Version	XM_006501462.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	FRAS1-related extracellular matrix protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501462.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC GTGCGCGCAG GACAGCCAAG TTCTCCAGCT TCCAGCCGAT ACTCGCACAG 
TCGCCTAGGC TGCTGCTGCT GCTGCTGCTC CTGTCACTGG TAAGCTACGT CTCCACACAG 
GCTGCTGGCC CCGGTGCAGC ACTTCAGTCC CTGGGTCTCT CCGGGACTTC GGGAGTCCCT 
ACAGAGGAAG CTATAGTGGT GGCAAACCGC GGGCTCCGTG TGCCCTTCGG TCGCGAAGTC 
TGGCTGGATC CGTTGCGTGA CTTGGTGCTG CAGGTGCAGC CAGGAGACCG CTGCACAGTG 
ACCGTGTTAG ACAACGACGC GCTGGCCCAG CGGCCGGGCC ACTTGAGTCC CAAGCGTTTT 
GCGTGCGACT ATGGCCCGGG TGAGGTGCGC TACTCGCACC TGGGTGCTCG CAGCCCTTCC 
CGCGACCGCG TCCGCCTGCA GCTGCGCTAC GATGCTCCAG GAGGGGCGAT AGTCCTGCCA 
CTGGCACTAG AGGTGGAAGT GGTCTTCACC CAGCTGGAGA TTGTGACTCG GAATTTACCT 
CTGGTCGTGG AAGAACTGCT AGGGACCAGT AATGCCTTGG ACGATCGGAG CTTAGAGTTC 
GCCTACCAGC CTGAGACCGA GGAGTGCCGC GTGGGCATCT TGTCAGGTCT GAGCGCTTTG 
CCTCGTTACG GAGAACTCCT TCACTACCCA CAGGTCCAGG GAGGGGCCGG AGATAGAGGG 
ACCTCTAAGA CCCTTTTGAT GGACTGCAAA GCATTCCAGG AGCTGGGAGT CCGCTACCGC 
CACACAGCCC CCAGCCGCTC GCCTAACAGG GACTGGCTGC CTATGGTGGT CGAGCTGCAT 
TCGCGAGGGG CTCCAGAGGG CAGCCCGGCT CTAAAACGCG AACACTTCCA GGTGCTTGTG 
AGGATTCGAG GAGGAGCGGA GAACACTGCA CCCAAGCCCA GTTTCGTGGC CATGATGATG 
ATGGAGGTGG ACCAGTTTGT ACTGACTGCT CTCACTCCAG ACATGCTAGC AGCCGAGGAT 
GCTGAATCCG ACCCAGACCT TTTGATTTTC AACCTCACCT CTGCCTTCCA GCCTGGGCAG 
GGCTACCTGG TGAGCACGGA TGACAGAAGC CTGCCTCTCT CCTCCTTTAC ACAGCGGGAT 
TTGCGCCTGC TGAAGATTGC CTACCAGCCT CCCTCTGAAG ACTCGGATCA GGAGCGTCTG 
TTTGAACTAG AGCTGGAGAT AGTGGACCCT GAAGGAGCAG CCTCAGACCC TTTCGCCTTC 
ATGGTGGTGG TGAAACCCAT GAACACACTG GCTCCCGTGG TCACACGAAA CACTGGCCTT 
ATTCTCTATG AGGGTCAATA TCGGCCGCTC ACGGGTCCCA TAGGCAGTGG ACCCCAGAAC 
TTGGTCATCA GTGATGAAGA TGACCTAGAA GCTGTGAGGC TGGAGGTGGT GGCTGGGCTC 
CGGCATGGCC ACCTTGTTAT TCTGGGTTCT CCCAGTAGCG ACTCTGCTCC CAAGACCTTC 
ACAGTGGCGG AGCTGGCGGC TGGCCAGGTT GTGTATCAGC ATGATGACAA AGACGGTTCA 
CTGAGTGACA ACTTGGTGCT GCGCATGTCC GATGGAGGAG GCAGGCACCA GGTCCAGTTC 
CTGTTCCCTA TCACTTTAGT GCCTGTGGAT GACCAGCCGC CGGTCCTGAA CGCCAACACT 
GGGCTGACAG TGGCTGAGGG GGAAACTGTG CCTATCCCAC CCTTGACTCT CAGTGCAACC 
GATATAGATT CAGATGACTC TCAGCTGGTT TTTGTCCTGT TGCCACCCTT CTCAAGCTTG 
GGGCACCTGC TTCTCCGCCA AAGGCATGTC CCCCAGGAAG AGCAGGGTCT GTGGCAGAAG 
CAAGGGTCAT TTTACGAGCG AACGGTGACA GAGTGGCGGC AACAGGATAT AACAGAGGGG 
AAGCTCTTCT ATAGGCACTC GGGGCCCCAC AGCCCTGGGC CGGTGATGGA CCAGTTCATG 
TTTAGAGTCC AAGACAATCA TGATCCCCCT AACCAGTCTG GGATCCAGAG GTTTGTAATA 
CGCATTCATC CCGTGGACCG TCTCCCTCCA GAACTGGGCA GTGGCTGTCC CCTTCGGATG 
GTGGTACAGG AATCCCAGCT CACACCACTG AGGAAAAGGT GGCTGCACTA CACTGACCTG 
GACACAGATG ACCGAGAGTT ACAGTACACA GTGACCCAGC CTCCCACGGA CACAGATGAG 
AACCACTCGC CGGCTCCACT GGGCACGTTG GTCTTCACTG ATAATCCCTC AGTGGTGGTG 
AGCCATTTTA CCCAAGCCCA GGTCAATCAT CATAAAATTG CTTACAGGCC CCCAGGGCAG 
GAGCTAGGAG TCGCAGCCCG AGTGGCTCAG TTCCAGTTCC AAGTAGAGGA TCGAGCTGGG 
AATGTGGCTC CTGGCACCTT CACCCTTTAC CTGCAGCCTG TGGATAACCA GCCCCCCGAG 
ATTGTCAACA CTGGCTTCAC CGTTGAGGAG AAGGGCCACC ACATCCTGCG TGAGACCGAG 
CTGCATGTGA GTGATGTTGA CACGGATGTG ACCCACATCT CGTTCACTCT CACACAGGCC 
CCCAAACACG GCCACATGCA AATATCTGGA CGGCCACTGC ATGTAGGCGG ACAATTCCAC 
TTGGAGGACA TAAAGCATGG CAGAATTTCC TACTGGAATA GCGGGGATGA GTCTTTGACT 
GACAGCTGCT CCCTGGAGGT CAGCGACAGA CACCATGTGG TGCCAATCAC TCTCAGAGTG 
AACGTTCGGC CAGGGGACCG TGAAGGTCCT ATGTCAGTCC TTCCTGCTGG TACTCTGGAG 
TCCTATCTAG ACGTCTTAGA GAATGGGGCT ACTGAAGTCA CTGCCAATAT CATTAAGGGG 
GCCTATCAGG GTACCGATGA TTTGATGCTG ACCTTCCTGT TAGAAGGCCC ACCCTCCTAT 
GGGGAAATCT TAGTCAACGG AGCTCCAGCT GAGCAGTTCA CCCAGAGGGA CATCCTGGAG 
GGCTCTGTTG TCTACGCTCA CACCAGTGGT GAGATTGGCC TGTTGCCCAA AGCTGACTCT 
TTTAACCTGA GTCTATCAGC TATGTCTCAA GAATGGAGAA TCGGTAGCAG CATCGTTCAA 
GGCGTCACTG TGTGGGTGAC CATCCTCCCC GTGGACAGCC AAGCCCCTGA GATTTCTCTT 
GGTGAACAGT TTGTAGTGCT GGAAGGTGAC AAGAGTGTGA TAAGCCTGAC CCACCTAAGT 
GCTGAGGACA TGGACTCCCT GAAGGATGAC CTCTTGTGCA CAATAGTCAT TCAGCCAACC 
TCAGGCTATG TAGAGAACAT TTCTCCAGCA CCAGGCTCAG AGAAATCAAG AGCCGGGGTT 
GCCATAAGTG CCTTCACGCT GAAGGATCTC AGGCAAGGAC ACATTAACTA TGTCCAGAGT 
GTCCACAGAG GAGTGGAGCC TGTCGAAGAC CGCTTTATAT TCCGTTGTTC TGACGGTATT 
AACTTCTCTG AGAGACAGAT ATTCCCCATT GTCATCATTC CTACCAATGA CGAGCAGCCA 
GAAATGTTCA TGAGGGAATT CATGGTGATG GAAGGCATGA GTCTGGTGGT CAACAGACTC 
ATCCTCAACG CTGCTGATGC CGACATTCCC AGGGATGATC TAACGTTTAC TATTACCCGG 
TTTCCAACTC ACGGTCATGT CATGAACCAG TTAATCAATG GTACAGTGTT GGTTGAAAGC 
TTCACCCTGG ATCAGATTAT AGAGAGTTCC AGCATTATTT ATGAGCACGA TGACTCTGAG 
ACACAAGAGG ACAGTTTTGT GATTAAACTG ACAGATGGCA AGCACTCCGT GGAGAAGATG 
GTCCTCATTG TGGTCATCCC TGTTGATGAC GAGACACCCA GAATGACCAT CAATAATGGG 
CTAGAAATAG AAATTGGGGA AACCAAAGTT ATCAACAACA AAGTCCTAAT GGCAACCGAT 
CTAGACTCTG ATGACAAATC ACTGGTTTAT ATTATCCGTT ATGGACCAGG GCATGGCTTG 
TTACAGAGAC AAAAACCTCT AGGTGCCTTT GAAAATATCA CTCTAGGCAT GAATTTTACC 
CAGGACGAAG TGGACAGGAA CTTGATTCAA TATGTCCACT TTGGGCAAGA AGGTATTCGA 
GACCTGATTA AATTCGATGT GACAGATGGA ACAAATGCTC TTATAGATCG CTACTTTTAC 
GTGACTATTG GAAGTGTTGA CATTGTCTTC CCTGATGTGG TAAGTAAGGG CGTGTCCTTG 
AAAGAAGGTG GCAAAGTCAC TCTGACAACG GATCTGCTGA GCACAAGTGA CTTGAACAGC 
CCTGATGAGA ACTTAGTTTT CACCATTACC AGGGCTCCTA TGAGAGGTCA CTTGGAGTGC 
ACGGATCGAA GGGGACTGTC CATCACTTCT TTCACTCAGC TCCAGCTGGC TGGTAACAAA 
ATCTACTACA TACACACGGC TGAGGATGAG GTCAAAATGG ACAGCTTCGA GTTTCAAGTC 
ACCGATGGGC GGAACCCTGT CTTCCGAACT TTCCGGATCT CAATCAGTGA CGTAGACAAC 
AAAAAGCCAG TGGTCACCAT CCACAACCTG GTGGTCAGTG AAAGTGAAAG TAAGCTGATC 
ACCCCCTTTG AACTCACTGT GGAGGACAGA GATACCCCTG ACAGGCTCCT AAAATTCATT 
GTCACCCAGG TCCCTGTTCA CGGTCACCTC CTCTTCAACA ATACCAGATC TGTCATGGTT 
TTCACCAAGC AAGACTTGAA TGAAAACTTA ATCAGCTACA AACACGATGG CACAGAATCC 
ACGGAAGACA GCTTCTCCTT CACCGTGACT GACGGCACCC ACTCGGACTT CTATGTTTTT 
CCTGATACAG TGTTTGAAAC CAGGAGACCT CAAGTGATGA AAATCCAGGT GTTACCTGTT 
GACAACAGTG TCCCCCAAAT TGTGGTGAAC AAAGGAGCTT CTACCCTTCG TACCCTGGCC 
ACTGGCCATT TAGGGTTCAT GATCACAAGC AAAATACTCA AAGTGGAGGA CAGAGACAGC 
CTGCATTTTT CTCTCAGGTT CATTGTGACA GAAGCCCCCC AACATGGCTA CCTTCTCAAC 
CTGGGCCAAG GAAACCACAG TGTCACACAG TTCACCCAAG CTGACATTGA TGACATGAAA 
ATATGCTATG TCTTGAGAGA GAGAGCAAAT GCCACAAGTG ATATGTTCCA TTTCATAGTG 
GAAGATGACG ATGAAAGATC CCATCCGCTG TGCAAGGGGA AGGGCTGTGT TTCTAACAGA 
GGCTGGACCC TGGCACCCAG GAGGCTCTCA CTGAGTAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501525.1
CDS	100..5421
Translation	MASRARRTAKFSSFQPILAQSPRLLLLLLLLSLVSYVSTQAAGPGAALQSLGLSGTSGVPTEEAIVVANRGLRVPFGREVWLDPLRDLVLQVQPGDRCTVTVLDNDALAQRPGHLSPKRFACDYGPGEVRYSHLGARSPSRDRVRLQLRYDAPGGAIVLPLALEVEVVFTQLEIVTRNLPLVVEELLGTSNALDDRSLEFAYQPETEECRVGILSGLSALPRYGELLHYPQVQGGAGDRGTSKTLLMDCKAFQELGVRYRHTAPSRSPNRDWLPMVVELHSRGAPEGSPALKREHFQVLVRIRGGAENTAPKPSFVAMMMMEVDQFVLTALTPDMLAAEDAESDPDLLIFNLTSAFQPGQGYLVSTDDRSLPLSSFTQRDLRLLKIAYQPPSEDSDQERLFELELEIVDPEGAASDPFAFMVVVKPMNTLAPVVTRNTGLILYEGQYRPLTGPIGSGPQNLVISDEDDLEAVRLEVVAGLRHGHLVILGSPSSDSAPKTFTVAELAAGQVVYQHDDKDGSLSDNLVLRMSDGGGRHQVQFLFPITLVPVDDQPPVLNANTGLTVAEGETVPIPPLTLSATDIDSDDSQLVFVLLPPFSSLGHLLLRQRHVPQEEQGLWQKQGSFYERTVTEWRQQDITEGKLFYRHSGPHSPGPVMDQFMFRVQDNHDPPNQSGIQRFVIRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKRWLHYTDLDTDDRELQYTVTQPPTDTDENHSPAPLGTLVFTDNPSVVVSHFTQAQVNHHKIAYRPPGQELGVAARVAQFQFQVEDRAGNVAPGTFTLYLQPVDNQPPEIVNTGFTVEEKGHHILRETELHVSDVDTDVTHISFTLTQAPKHGHMQISGRPLHVGGQFHLEDIKHGRISYWNSGDESLTDSCSLEVSDRHHVVPITLRVNVRPGDREGPMSVLPAGTLESYLDVLENGATEVTANIIKGAYQGTDDLMLTFLLEGPPSYGEILVNGAPAEQFTQRDILEGSVVYAHTSGEIGLLPKADSFNLSLSAMSQEWRIGSSIVQGVTVWVTILPVDSQAPEISLGEQFVVLEGDKSVISLTHLSAEDMDSLKDDLLCTIVIQPTSGYVENISPAPGSEKSRAGVAISAFTLKDLRQGHINYVQSVHRGVEPVEDRFIFRCSDGINFSERQIFPIVIIPTNDEQPEMFMREFMVMEGMSLVVNRLILNAADADIPRDDLTFTITRFPTHGHVMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGKHSVEKMVLIVVIPVDDETPRMTINNGLEIEIGETKVINNKVLMATDLDSDDKSLVYIIRYGPGHGLLQRQKPLGAFENITLGMNFTQDEVDRNLIQYVHFGQEGIRDLIKFDVTDGTNALIDRYFYVTIGSVDIVFPDVVSKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLECTDRRGLSITSFTQLQLAGNKIYYIHTAEDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHNLVVSESESKLITPFELTVEDRDTPDRLLKFIVTQVPVHGHLLFNNTRSVMVFTKQDLNENLISYKHDGTESTEDSFSFTVTDGTHSDFYVFPDTVFETRRPQVMKIQVLPVDNSVPQIVVNKGASTLRTLATGHLGFMITSKILKVEDRDSLHFSLRFIVTEAPQHGYLLNLGQGNHSVTQFTQADIDDMKICYVLRERANATSDMFHFIVEDDDERSHPLCKGKGCVSNRGWTLAPRRLSLSS

Target ORF information:

RefSeq Version	XM_006501462.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501462.3

ORF Insert Sequence:

ATGGCCAGCC GTGCGCGCAG GACAGCCAAG TTCTCCAGCT TCCAGCCGAT ACTCGCACAG 
TCGCCTAGGC TGCTGCTGCT GCTGCTGCTC CTGTCACTGG TAAGCTACGT CTCCACACAG 
GCTGCTGGCC CCGGTGCAGC ACTTCAGTCC CTGGGTCTCT CCGGGACTTC GGGAGTCCCT 
ACAGAGGAAG CTATAGTGGT GGCAAACCGC GGGCTCCGTG TGCCCTTCGG TCGCGAAGTC 
TGGCTGGATC CGTTGCGTGA CTTGGTGCTG CAGGTGCAGC CAGGAGACCG CTGCACAGTG 
ACCGTGTTAG ACAACGACGC GCTGGCCCAG CGGCCGGGCC ACTTGAGTCC CAAGCGTTTT 
GCGTGCGACT ATGGCCCGGG TGAGGTGCGC TACTCGCACC TGGGTGCTCG CAGCCCTTCC 
CGCGACCGCG TCCGCCTGCA GCTGCGCTAC GATGCTCCAG GAGGGGCGAT AGTCCTGCCA 
CTGGCACTAG AGGTGGAAGT GGTCTTCACC CAGCTGGAGA TTGTGACTCG GAATTTACCT 
CTGGTCGTGG AAGAACTGCT AGGGACCAGT AATGCCTTGG ACGATCGGAG CTTAGAGTTC 
GCCTACCAGC CTGAGACCGA GGAGTGCCGC GTGGGCATCT TGTCAGGTCT GAGCGCTTTG 
CCTCGTTACG GAGAACTCCT TCACTACCCA CAGGTCCAGG GAGGGGCCGG AGATAGAGGG 
ACCTCTAAGA CCCTTTTGAT GGACTGCAAA GCATTCCAGG AGCTGGGAGT CCGCTACCGC 
CACACAGCCC CCAGCCGCTC GCCTAACAGG GACTGGCTGC CTATGGTGGT CGAGCTGCAT 
TCGCGAGGGG CTCCAGAGGG CAGCCCGGCT CTAAAACGCG AACACTTCCA GGTGCTTGTG 
AGGATTCGAG GAGGAGCGGA GAACACTGCA CCCAAGCCCA GTTTCGTGGC CATGATGATG 
ATGGAGGTGG ACCAGTTTGT ACTGACTGCT CTCACTCCAG ACATGCTAGC AGCCGAGGAT 
GCTGAATCCG ACCCAGACCT TTTGATTTTC AACCTCACCT CTGCCTTCCA GCCTGGGCAG 
GGCTACCTGG TGAGCACGGA TGACAGAAGC CTGCCTCTCT CCTCCTTTAC ACAGCGGGAT 
TTGCGCCTGC TGAAGATTGC CTACCAGCCT CCCTCTGAAG ACTCGGATCA GGAGCGTCTG 
TTTGAACTAG AGCTGGAGAT AGTGGACCCT GAAGGAGCAG CCTCAGACCC TTTCGCCTTC 
ATGGTGGTGG TGAAACCCAT GAACACACTG GCTCCCGTGG TCACACGAAA CACTGGCCTT 
ATTCTCTATG AGGGTCAATA TCGGCCGCTC ACGGGTCCCA TAGGCAGTGG ACCCCAGAAC 
TTGGTCATCA GTGATGAAGA TGACCTAGAA GCTGTGAGGC TGGAGGTGGT GGCTGGGCTC 
CGGCATGGCC ACCTTGTTAT TCTGGGTTCT CCCAGTAGCG ACTCTGCTCC CAAGACCTTC 
ACAGTGGCGG AGCTGGCGGC TGGCCAGGTT GTGTATCAGC ATGATGACAA AGACGGTTCA 
CTGAGTGACA ACTTGGTGCT GCGCATGTCC GATGGAGGAG GCAGGCACCA GGTCCAGTTC 
CTGTTCCCTA TCACTTTAGT GCCTGTGGAT GACCAGCCGC CGGTCCTGAA CGCCAACACT 
GGGCTGACAG TGGCTGAGGG GGAAACTGTG CCTATCCCAC CCTTGACTCT CAGTGCAACC 
GATATAGATT CAGATGACTC TCAGCTGGTT TTTGTCCTGT TGCCACCCTT CTCAAGCTTG 
GGGCACCTGC TTCTCCGCCA AAGGCATGTC CCCCAGGAAG AGCAGGGTCT GTGGCAGAAG 
CAAGGGTCAT TTTACGAGCG AACGGTGACA GAGTGGCGGC AACAGGATAT AACAGAGGGG 
AAGCTCTTCT ATAGGCACTC GGGGCCCCAC AGCCCTGGGC CGGTGATGGA CCAGTTCATG 
TTTAGAGTCC AAGACAATCA TGATCCCCCT AACCAGTCTG GGATCCAGAG GTTTGTAATA 
CGCATTCATC CCGTGGACCG TCTCCCTCCA GAACTGGGCA GTGGCTGTCC CCTTCGGATG 
GTGGTACAGG AATCCCAGCT CACACCACTG AGGAAAAGGT GGCTGCACTA CACTGACCTG 
GACACAGATG ACCGAGAGTT ACAGTACACA GTGACCCAGC CTCCCACGGA CACAGATGAG 
AACCACTCGC CGGCTCCACT GGGCACGTTG GTCTTCACTG ATAATCCCTC AGTGGTGGTG 
AGCCATTTTA CCCAAGCCCA GGTCAATCAT CATAAAATTG CTTACAGGCC CCCAGGGCAG 
GAGCTAGGAG TCGCAGCCCG AGTGGCTCAG TTCCAGTTCC AAGTAGAGGA TCGAGCTGGG 
AATGTGGCTC CTGGCACCTT CACCCTTTAC CTGCAGCCTG TGGATAACCA GCCCCCCGAG 
ATTGTCAACA CTGGCTTCAC CGTTGAGGAG AAGGGCCACC ACATCCTGCG TGAGACCGAG 
CTGCATGTGA GTGATGTTGA CACGGATGTG ACCCACATCT CGTTCACTCT CACACAGGCC 
CCCAAACACG GCCACATGCA AATATCTGGA CGGCCACTGC ATGTAGGCGG ACAATTCCAC 
TTGGAGGACA TAAAGCATGG CAGAATTTCC TACTGGAATA GCGGGGATGA GTCTTTGACT 
GACAGCTGCT CCCTGGAGGT CAGCGACAGA CACCATGTGG TGCCAATCAC TCTCAGAGTG 
AACGTTCGGC CAGGGGACCG TGAAGGTCCT ATGTCAGTCC TTCCTGCTGG TACTCTGGAG 
TCCTATCTAG ACGTCTTAGA GAATGGGGCT ACTGAAGTCA CTGCCAATAT CATTAAGGGG 
GCCTATCAGG GTACCGATGA TTTGATGCTG ACCTTCCTGT TAGAAGGCCC ACCCTCCTAT 
GGGGAAATCT TAGTCAACGG AGCTCCAGCT GAGCAGTTCA CCCAGAGGGA CATCCTGGAG 
GGCTCTGTTG TCTACGCTCA CACCAGTGGT GAGATTGGCC TGTTGCCCAA AGCTGACTCT 
TTTAACCTGA GTCTATCAGC TATGTCTCAA GAATGGAGAA TCGGTAGCAG CATCGTTCAA 
GGCGTCACTG TGTGGGTGAC CATCCTCCCC GTGGACAGCC AAGCCCCTGA GATTTCTCTT 
GGTGAACAGT TTGTAGTGCT GGAAGGTGAC AAGAGTGTGA TAAGCCTGAC CCACCTAAGT 
GCTGAGGACA TGGACTCCCT GAAGGATGAC CTCTTGTGCA CAATAGTCAT TCAGCCAACC 
TCAGGCTATG TAGAGAACAT TTCTCCAGCA CCAGGCTCAG AGAAATCAAG AGCCGGGGTT 
GCCATAAGTG CCTTCACGCT GAAGGATCTC AGGCAAGGAC ACATTAACTA TGTCCAGAGT 
GTCCACAGAG GAGTGGAGCC TGTCGAAGAC CGCTTTATAT TCCGTTGTTC TGACGGTATT 
AACTTCTCTG AGAGACAGAT ATTCCCCATT GTCATCATTC CTACCAATGA CGAGCAGCCA 
GAAATGTTCA TGAGGGAATT CATGGTGATG GAAGGCATGA GTCTGGTGGT CAACAGACTC 
ATCCTCAACG CTGCTGATGC CGACATTCCC AGGGATGATC TAACGTTTAC TATTACCCGG 
TTTCCAACTC ACGGTCATGT CATGAACCAG TTAATCAATG GTACAGTGTT GGTTGAAAGC 
TTCACCCTGG ATCAGATTAT AGAGAGTTCC AGCATTATTT ATGAGCACGA TGACTCTGAG 
ACACAAGAGG ACAGTTTTGT GATTAAACTG ACAGATGGCA AGCACTCCGT GGAGAAGATG 
GTCCTCATTG TGGTCATCCC TGTTGATGAC GAGACACCCA GAATGACCAT CAATAATGGG 
CTAGAAATAG AAATTGGGGA AACCAAAGTT ATCAACAACA AAGTCCTAAT GGCAACCGAT 
CTAGACTCTG ATGACAAATC ACTGGTTTAT ATTATCCGTT ATGGACCAGG GCATGGCTTG 
TTACAGAGAC AAAAACCTCT AGGTGCCTTT GAAAATATCA CTCTAGGCAT GAATTTTACC 
CAGGACGAAG TGGACAGGAA CTTGATTCAA TATGTCCACT TTGGGCAAGA AGGTATTCGA 
GACCTGATTA AATTCGATGT GACAGATGGA ACAAATGCTC TTATAGATCG CTACTTTTAC 
GTGACTATTG GAAGTGTTGA CATTGTCTTC CCTGATGTGG TAAGTAAGGG CGTGTCCTTG 
AAAGAAGGTG GCAAAGTCAC TCTGACAACG GATCTGCTGA GCACAAGTGA CTTGAACAGC 
CCTGATGAGA ACTTAGTTTT CACCATTACC AGGGCTCCTA TGAGAGGTCA CTTGGAGTGC 
ACGGATCGAA GGGGACTGTC CATCACTTCT TTCACTCAGC TCCAGCTGGC TGGTAACAAA 
ATCTACTACA TACACACGGC TGAGGATGAG GTCAAAATGG ACAGCTTCGA GTTTCAAGTC 
ACCGATGGGC GGAACCCTGT CTTCCGAACT TTCCGGATCT CAATCAGTGA CGTAGACAAC 
AAAAAGCCAG TGGTCACCAT CCACAACCTG GTGGTCAGTG AAAGTGAAAG TAAGCTGATC 
ACCCCCTTTG AACTCACTGT GGAGGACAGA GATACCCCTG ACAGGCTCCT AAAATTCATT 
GTCACCCAGG TCCCTGTTCA CGGTCACCTC CTCTTCAACA ATACCAGATC TGTCATGGTT 
TTCACCAAGC AAGACTTGAA TGAAAACTTA ATCAGCTACA AACACGATGG CACAGAATCC 
ACGGAAGACA GCTTCTCCTT CACCGTGACT GACGGCACCC ACTCGGACTT CTATGTTTTT 
CCTGATACAG TGTTTGAAAC CAGGAGACCT CAAGTGATGA AAATCCAGGT GTTACCTGTT 
GACAACAGTG TCCCCCAAAT TGTGGTGAAC AAAGGAGCTT CTACCCTTCG TACCCTGGCC 
ACTGGCCATT TAGGGTTCAT GATCACAAGC AAAATACTCA AAGTGGAGGA CAGAGACAGC 
CTGCATTTTT CTCTCAGGTT CATTGTGACA GAAGCCCCCC AACATGGCTA CCTTCTCAAC 
CTGGGCCAAG GAAACCACAG TGTCACACAG TTCACCCAAG CTGACATTGA TGACATGAAA 
ATATGCTATG TCTTGAGAGA GAGAGCAAAT GCCACAAGTG ATATGTTCCA TTTCATAGTG 
GAAGATGACG ATGAAAGATC CCATCCGCTG TGCAAGGGGA AGGGCTGTGT TTCTAACAGA 
GGCTGGACCC TGGCACCCAG GAGGCTCTCA CTGAGTAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frem2 ( NM_172862.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766450.2 Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), mRNA.

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CloneID	OMu15006
Clone ID Related Accession (Same CDS sequence)	NM_172862.3
Accession Version	NM_172862.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	FRAS1-related extracellular matrix protein 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC138112.4, CN536459.1, CN534900.1, CN530725.1, CF742372.1, BC068185.1 and BE864729.1. On Jul 28, 2006 this sequence version replaced NM_172862.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB236662.1, AJ833643.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCCAGCC GTGCGCGCAG GACAGCCAAG TTCTCCAGCT TCCAGCCGAT ACTCGCACAG 
TCGCCTAGGC TGCTGCTGCT GCTGCTGCTC CTGTCACTGG TAAGCTACGT CTCCACACAG 
GCTGCTGGCC CCGGTGCAGC ACTTCAGTCC CTGGGTCTCT CCGGGACTTC GGGAGTCCCT 
ACAGAGGAAG CTATAGTGGT GGCAAACCGC GGGCTCCGTG TGCCCTTCGG TCGCGAAGTC 
TGGCTGGATC CGTTGCGTGA CTTGGTGCTG CAGGTGCAGC CAGGAGACCG CTGCACAGTG 
ACCGTGTTAG ACAACGACGC GCTGGCCCAG CGGCCGGGCC ACTTGAGTCC CAAGCGTTTT 
GCGTGCGACT ATGGCCCGGG TGAGGTGCGC TACTCGCACC TGGGTGCTCG CAGCCCTTCC 
CGCGACCGCG TCCGCCTGCA GCTGCGCTAC GATGCTCCAG GAGGGGCGAT AGTCCTGCCA 
CTGGCACTAG AGGTGGAAGT GGTCTTCACC CAGCTGGAGA TTGTGACTCG GAATTTACCT 
CTGGTCGTGG AAGAACTGCT AGGGACCAGT AATGCCTTGG ACGATCGGAG CTTAGAGTTC 
GCCTACCAGC CTGAGACCGA GGAGTGCCGC GTGGGCATCT TGTCAGGTCT GAGCGCTTTG 
CCTCGTTACG GAGAACTCCT TCACTACCCA CAGGTCCAGG GAGGGGCCGG AGATAGAGGG 
ACCTCTAAGA CCCTTTTGAT GGACTGCAAA GCATTCCAGG AGCTGGGAGT CCGCTACCGC 
CACACAGCCC CCAGCCGCTC GCCTAACAGG GACTGGCTGC CTATGGTGGT CGAGCTGCAT 
TCGCGAGGGG CTCCAGAGGG CAGCCCGGCT CTAAAACGCG AACACTTCCA GGTGCTTGTG 
AGGATTCGAG GAGGAGCGGA GAACACTGCA CCCAAGCCCA GTTTCGTGGC CATGATGATG 
ATGGAGGTGG ACCAGTTTGT ACTGACTGCT CTCACTCCAG ACATGCTAGC AGCCGAGGAT 
GCTGAATCCG ACCCAGACCT TTTGATTTTC AACCTCACCT CTGCCTTCCA GCCTGGGCAG 
GGCTACCTGG TGAGCACGGA TGACAGAAGC CTGCCTCTCT CCTCCTTTAC ACAGCGGGAT 
TTGCGCCTGC TGAAGATTGC CTACCAGCCT CCCTCTGAAG ACTCGGATCA GGAGCGTCTG 
TTTGAACTAG AGCTGGAGAT AGTGGACCCT GAAGGAGCAG CCTCAGACCC TTTCGCCTTC 
ATGGTGGTGG TGAAACCCAT GAACACACTG GCTCCCGTGG TCACACGAAA CACTGGCCTT 
ATTCTCTATG AGGGTCAATA TCGGCCGCTC ACGGGTCCCA TAGGCAGTGG ACCCCAGAAC 
TTGGTCATCA GTGATGAAGA TGACCTAGAA GCTGTGAGGC TGGAGGTGGT GGCTGGGCTC 
CGGCATGGCC ACCTTGTTAT TCTGGGTTCT CCCAGTAGCG ACTCTGCTCC CAAGACCTTC 
ACAGTGGCGG AGCTGGCGGC TGGCCAGGTT GTGTATCAGC ATGATGACAA AGACGGTTCA 
CTGAGTGACA ACTTGGTGCT GCGCATGTCC GATGGAGGAG GCAGGCACCA GGTCCAGTTC 
CTGTTCCCTA TCACTTTAGT GCCTGTGGAT GACCAGCCGC CGGTCCTGAA CGCCAACACT 
GGGCTGACAG TGGCTGAGGG GGAAACTGTG CCTATCCCAC CCTTGACTCT CAGTGCAACC 
GATATAGATT CAGATGACTC TCAGCTGGTT TTTGTCCTGT TGCCACCCTT CTCAAGCTTG 
GGGCACCTGC TTCTCCGCCA AAGGCATGTC CCCCAGGAAG AGCAGGGTCT GTGGCAGAAG 
CAAGGGTCAT TTTACGAGCG AACGGTGACA GAGTGGCGGC AACAGGATAT AACAGAGGGG 
AAGCTCTTCT ATAGGCACTC GGGGCCCCAC AGCCCTGGGC CGGTGATGGA CCAGTTCATG 
TTTAGAGTCC AAGACAATCA TGATCCCCCT AACCAGTCTG GGATCCAGAG GTTTGTAATA 
CGCATTCATC CCGTGGACCG TCTCCCTCCA GAACTGGGCA GTGGCTGTCC CCTTCGGATG 
GTGGTACAGG AATCCCAGCT CACACCACTG AGGAAAAGGT GGCTGCACTA CACTGACCTG 
GACACAGATG ACCGAGAGTT ACAGTACACA GTGACCCAGC CTCCCACGGA CACAGATGAG 
AACCACTCGC CGGCTCCACT GGGCACGTTG GTCTTCACTG ATAATCCCTC AGTGGTGGTG 
AGCCATTTTA CCCAAGCCCA GGTCAATCAT CATAAAATTG CTTACAGGCC CCCAGGGCAG 
GAGCTAGGAG TCGCAGCCCG AGTGGCTCAG TTCCAGTTCC AAGTAGAGGA TCGAGCTGGG 
AATGTGGCTC CTGGCACCTT CACCCTTTAC CTGCAGCCTG TGGATAACCA GCCCCCCGAG 
ATTGTCAACA CTGGCTTCAC CGTTGAGGAG AAGGGCCACC ACATCCTGCG TGAGACCGAG 
CTGCATGTGA GTGATGTTGA CACGGATGTG ACCCACATCT CGTTCACTCT CACACAGGCC 
CCCAAACACG GCCACATGCA AATATCTGGA CGGCCACTGC ATGTAGGCGG ACAATTCCAC 
TTGGAGGACA TAAAGCATGG CAGAATTTCC TACTGGAATA GCGGGGATGA GTCTTTGACT 
GACAGCTGCT CCCTGGAGGT CAGCGACAGA CACCATGTGG TGCCAATCAC TCTCAGAGTG 
AACGTTCGGC CAGGGGACCG TGAAGGTCCT ATGTCAGTCC TTCCTGCTGG TACTCTGGAG 
TCCTATCTAG ACGTCTTAGA GAATGGGGCT ACTGAAGTCA CTGCCAATAT CATTAAGGGG 
GCCTATCAGG GTACCGATGA TTTGATGCTG ACCTTCCTGT TAGAAGGCCC ACCCTCCTAT 
GGGGAAATCT TAGTCAACGG AGCTCCAGCT GAGCAGTTCA CCCAGAGGGA CATCCTGGAG 
GGCTCTGTTG TCTACGCTCA CACCAGTGGT GAGATTGGCC TGTTGCCCAA AGCTGACTCT 
TTTAACCTGA GTCTATCAGC TATGTCTCAA GAATGGAGAA TCGGTAGCAG CATCGTTCAA 
GGCGTCACTG TGTGGGTGAC CATCCTCCCC GTGGACAGCC AAGCCCCTGA GATTTCTCTT 
GGTGAACAGT TTGTAGTGCT GGAAGGTGAC AAGAGTGTGA TAAGCCTGAC CCACCTAAGT 
GCTGAGGACA TGGACTCCCT GAAGGATGAC CTCTTGTGCA CAATAGTCAT TCAGCCAACC 
TCAGGCTATG TAGAGAACAT TTCTCCAGCA CCAGGCTCAG AGAAATCAAG AGCCGGGGTT 
GCCATAAGTG CCTTCACGCT GAAGGATCTC AGGCAAGGAC ACATTAACTA TGTCCAGAGT 
GTCCACAGAG GAGTGGAGCC TGTCGAAGAC CGCTTTATAT TCCGTTGTTC TGACGGTATT 
AACTTCTCTG AGAGACAGAT ATTCCCCATT GTCATCATTC CTACCAATGA CGAGCAGCCA 
GAAATGTTCA TGAGGGAATT CATGGTGATG GAAGGCATGA GTCTGGTGGT CAACAGACTC 
ATCCTCAACG CTGCTGATGC CGACATTCCC AGGGATGATC TAACGTTTAC TATTACCCGG 
TTTCCAACTC ACGGTCATGT CATGAACCAG TTAATCAATG GTACAGTGTT GGTTGAAAGC 
TTCACCCTGG ATCAGATTAT AGAGAGTTCC AGCATTATTT ATGAGCACGA TGACTCTGAG 
ACACAAGAGG ACAGTTTTGT GATTAAACTG ACAGATGGCA AGCACTCCGT GGAGAAGATG 
GTCCTCATTG TGGTCATCCC TGTTGATGAC GAGACACCCA GAATGACCAT CAATAATGGG 
CTAGAAATAG AAATTGGGGA AACCAAAGTT ATCAACAACA AAGTCCTAAT GGCAACCGAT 
CTAGACTCTG ATGACAAATC ACTGGTTTAT ATTATCCGTT ATGGACCAGG GCATGGCTTG 
TTACAGAGAC AAAAACCTCT AGGTGCCTTT GAAAATATCA CTCTAGGCAT GAATTTTACC 
CAGGACGAAG TGGACAGGAA CTTGATTCAA TATGTCCACT TTGGGCAAGA AGGTATTCGA 
GACCTGATTA AATTCGATGT GACAGATGGA ACAAATGCTC TTATAGATCG CTACTTTTAC 
GTGACTATTG GAAGTGTTGA CATTGTCTTC CCTGATGTGG TAAGTAAGGG CGTGTCCTTG 
AAAGAAGGTG GCAAAGTCAC TCTGACAACG GATCTGCTGA GCACAAGTGA CTTGAACAGC 
CCTGATGAGA ACTTAGTTTT CACCATTACC AGGGCTCCTA TGAGAGGTCA CTTGGAGTGC 
ACGGATCGAA GGGGACTGTC CATCACTTCT TTCACTCAGC TCCAGCTGGC TGGTAACAAA 
ATCTACTACA TACACACGGC TGAGGATGAG GTCAAAATGG ACAGCTTCGA GTTTCAAGTC 
ACCGATGGGC GGAACCCTGT CTTCCGAACT TTCCGGATCT CAATCAGTGA CGTAGACAAC 
AAAAAGCCAG TGGTCACCAT CCACAACCTG GTGGTCAGTG AAAGTGAAAG TAAGCTGATC 
ACCCCCTTTG AACTCACTGT GGAGGACAGA GATACCCCTG ACAGGCTCCT AAAATTCATT 
GTCACCCAGG TCCCTGTTCA CGGTCACCTC CTCTTCAACA ATACCAGATC TGTCATGGTT 
TTCACCAAGC AAGACTTGAA TGAAAACTTA ATCAGCTACA AACACGATGG CACAGAATCC 
ACGGAAGACA GCTTCTCCTT CACCGTGACT GACGGCACCC ACTCGGACTT CTATGTTTTT 
CCTGATACAG TGTTTGAAAC CAGGAGACCT CAAGTGATGA AAATCCAGGT GTTACCTGTT 
GACAACAGTG TCCCCCAAAT TGTGGTGAAC AAAGGAGCTT CTACCCTTCG TACCCTGGCC 
ACTGGCCATT TAGGGTTCAT GATCACAAGC AAAATACTCA AAGTGGAGGA CAGAGACAGC 
CTGCATTTTT CTCTCAGGTT CATTGTGACA GAAGCCCCCC AACATGGCTA CCTTCTCAAC 
CTGGGCCAAG GAAACCACAG TGTCACACAG TTCACCCAAG CTGACATTGA TGACATGAAA 
ATATGCTATG TCTTGAGAGA GAGAGCAAAT GCCACAAGTG ATATGTTCCA TTTCATAGTG 
GAAGATGACG GTGGAAACAG GTTAACCAAC CAGCATTTTC GACTGAATTG GGCGTGGATC 
TCCTTTGAAA AAGAGTATTA CTTGATCAAC GAAGACTCCA AATTTCTAGA TATTGTTCTT 
ACACGCAGAG GATACTTGGG AGAAACTTCA TTTATAAGTA TCGGCACCAG GGATGGGACT 
GCGGAAAAAG ACAGAGACTT CAAGGGTAAA GCTCAGAAAC AGGTACAGTT CAACCCCGGC 
CAGACCAGGG CCTCGTGGAG AGTGCGCATC CTGAGCGACG GGGAACACGA GCACTCGGAG 
ACCTTCCAAG TGGTTCTCTC GGAGCCTGTG CTGGCAATCC TGGAGTTCCC CACAGTGACT 
ACAGTGGAGA TCATCGACCC AGGAGACGAA TCAACCGTGT TCATTCCCCA GTCTGAGTAC 
TCTGTTGAAG AAGATGTGGG TGAGCTGTTC ATTCCCATCA GGAGAAGTGG AGACATCAGC 
CGGGAGCTGA TGGTGATCTG CTACACACAG CAAGGGACAG CCACCAGCAC TGTGCGGACG 
TCCGTGCTCT CTTACTCTGA CTACATCTCC AGGCCCGAGG ACCACAGCAG CGTGATCCGC 
TTTGACAAAG ACGAACGAGA GAAAATGTGT CGTATTTTGG TAATTGATGA CTCTTTGTAC 
GAAGAGGAGG AGACTTTCCA GGTCCTTTTG AGCATGCCCA TGGGCGGGAG GATCGGTGAC 
AAGTTTCCAG GAGCCAATGT GACCATCCTC ACAGACAGGG ATGATGAGCC TGCCTTCTAC 
TTTGGGGACA CCCAGTACTC CGTGGATGAG AGTGCCGGCT ACGTGGAGCT GCAGGTGTGG 
AGGACGGGGA CCGACCTCTC TAAGCCTTCC AGTGTCACAG TGAGGTCCCG GAAAACAGAA 
TCCCTCTCTG CAGATGCTGG AACAGACTAT GTGGGAATCA GTCGCAATTT AGATTTTGCG 
CCTGGCGTCA ACATGCAGAC TGTTCGAGTG GTCATTCTGG ATGACCTTGG ACGACCAATA 
CTGGAGGGAA TTGAGAAGTT TGAACTGGTA CTTCGAATGC CTATGAACGC TGCCCTTGGG 
GAGCCCAGCA AGGCCACCGT GTCCATAAAT GACTCTGCCT CAGACTTGCC TAAAATGCAA 
TTCAAAGAGA GAGTGTACAC TTGCAACGAG AATGACGGGC GTGTAGTGGC CATGATCTAC 
AGGAGCGGTG ACATCCAGCA CAGGTCTTCT GTGAGATGTT ACACGAGGCA GGGGTCTGCG 
CAGGTGATGA TGGACTTCGA GGAACGCCCA AATACCGATG TTTCCACTGT CACATTCCTC 
CCTGGTGAGA TGGAGAAGCC GTGTGTCCTC GAGCTGATGG ATGATGCTGT CTATGAGGAT 
GTAGAGGAGC TGCGCCTGGT ACTTGGCACG CCTCAGGGCA GCTCTGCCTT TGGAGCTGCA 
GTGGGCGAGC AGAACGAAAC CCTGATAAAG ATCCAGGATG AGGCTGACAA AGCTGTTATC 
AAATTTGGAG AAACCAAATT TAGTGTCACT GAGCCCAGCA GACCAGGAGA GTCGGTGGTT 
GTCAAAATCC CGGTCATCCG TCAAGGAGAC ACTTCAAAGG TCTCCATCGT GAGAGTCCAC 
ACCAAGGATG GCTCGGCTAC CTCCGGAGAG GATTATCACC CTGTGTCAGA AGAAATCGAG 
TTTAAGGAGG GAGAAACCCA ACACACTGTT GAAATTGAAG TAATCTTCGA CGGGGTGAGA 
GAGATGAGGG AGGCCTTCAC TGTTCACCTA AAACCAGATG AAAATATGGT GGCAGAGACA 
CAGGCAACCA AGGCCATTGT ATATATAGAA GAAATCCACA GCATGGCAGA TGTCACCTTT 
CCTTCTGTCC CTCACATTGT GTCCTTGCTG ATATACGATG ATCCCTCCAA AGGCAGAGAG 
GACACTGGAC CTGTGTCTGG CTACCCGGTT GTCTGCATTA CGGCCTGCAA CCCCAAATAT 
CCAGACTATG AGAAAACAGG CTCTATTTGT GCCAGTGAGA ACATCAACGA CACTTTGACC 
CGATACAGAT GGCTGATCAG CGCTCCCGCT GGCCCTGACG GTGTGACCAG CCCCATGAGA 
GAGGTCGACT TCGACACCTT CTTCACATCA TCCAAAATGA TTACATTAGA CTCCATATAC 
TTTCAGCCTG GCTCCCGGGT CCAATGTGCG GCCCGAGCAG TGAACACCAA TGGGAATGAA 
GGCCTGGAAC TAATGAGCCC CATCGTTACC ATCGGCAGAG AGGAAGGTCT CTGTCAGCCC 
CGTGTGCCTG GGGTAGTGGG GGCAGAGCCA TTCTCCGCTA AGTTGCGCTA CACTGGCCCA 
GAGGACCCAG ACTTTGCCAA CCTCATCAAA CTCACTGTCA CTATGCCGCA CATAGACGGC 
ATGCTCCCTG CCATCTCCAC AAGAGAGCTG TCCAACTTTG AGCTGACCCT CAGCCCTGAC 
GGCACGAGAG TTGGGAATCA CAAATGCTCC AACCTGCTGG ACTACAATGA AGTAAAGACC 
CACCATGGCT TCCTGACCAA CGCCACAAAG AACCCAGAGG TCATTGGAGA GACCTATCCT 
TACCAGTACA GTGTGCCGGT CAGGGGTTCC AGCACCTTAC GCTTCTACCG AAACCTGAAC 
CTGGAAGCAT GTCTGTGGGA GTTTGTCAGC TACTATGACA TGACGGAGCT CCTGGCTGAC 
TGTGGAGGAA CCATCGGGAC CGATGGCCAG GTCCTCAACC TCGTGCAGTC CTACGTGACC 
CTTCGAGTTC CTCTGTACGT TTCCTACGTG TTCCACTCCC CCGTGGGCGT GGGCGGCTGG 
CAGCATTTCG ACTTGAAGTC AGAGCTCCGA CTCACATTCG TGTACGACAC TGCCATCTTG 
TGGAACCATG GGATCGGCAG CCCCCCGGAA GCTGAGCTGC AAGGCTCTCT TTACCCAACC 
AGCATGCGCA TTGGTGAGGA GGGACGCCTG GCTGTGAACT TCAAAACAGA GGCTCAGTTC 
CATGGCTTGT TTGTGCTGTC CCATCCTGCT TCCTTTACCA GCTCACTGAT CGTGTCAGCT 
GACCATCCGG GCCTCACATT TTCCCTCCGC CTTATACGGA GTGAACCAAC CTACAATCAG 
CCAGTCCAGC AGTGGAGTTT CGTGTCAGAC TTTGCGGTCC GTGACTACTC GGGCACCTAC 
ACAGTAAAGT TGGTACCCTG CACCACTCCA TCCAACCAGG AGTACCGTCT GCCAGTCACC 
TGCAACCCCA GAGAGCCTGT CACCTTTGAC CTTGACATTC GATTCCAACA GGTCAGCGAT 
CCCGTGGCGA CTGAGTTCAG CCTGAACACC CACATGTATC TGCTGTCTAA GAAGAATCTC 
TGGTTGTCTG ATGGGTCCAT GGGATTTGGG CAGGAAAGCG ACGTGGCTTT TGCAGAAGGG 
GATGTGATTT ATGGCCGTGT CATGGTCGAT CCTGTCCAGA ACCTCGGTGA CTCCTTTTAC 
TGCAGCATTG AGAAGGTGTT CTTATGCACG GGGGATGACG GCTACGTGCC CAAGTACAGC 
CCAGCGAATG CGGAATATGG CTGCCTGGCT GATTCTCCTT CACTCTTACA TAGATTCAAA 
ATCGTGGATA AGGCTCAGCC AGAGACACAA GCCACCAGCT TTGGAGATGT CCTGTTTAAC 
GCCAAACTGG CAGTGGATGA CCCTGAAGCT GTTCTCTTAG TGAATCAGCC TGGATCCGAT 
GGTTTTAAAG TAGACTCAAC ACCACTCTTT CAGGTTGCCT TGGGCCGAGA GTGGTACATA 
CACACAATCT ATACGGTAAA GTCTAAGGAC AACACCCATC GAGGCATTGG CAAGAGGAGC 
CTGGAGTACC AGTATCACTC AGTGGTGCAT CCCGGACCAC CGCAGGCAAC CACCAAGAGC 
TGGAAGAAGA GAGCAGTCAG GAGCACGCCC TCCCTCGCAG GGGAGATCGG TGCTGAGAAC 
AACCGGGGAA CAAACATCCA ACATATCTCC CTGAACCGCC GTGGCAAGAG GCAGGTCCCC 
CACGGAAGAA TTCCTCCTGA CGGCATCCTG CCCTGGGAGC TCAATAGTCC CAGCTCTGAA 
GTGAGCCTTG TCACTGTCCT GGGAGGCCTC ACGGTGGGCT TGCTCACAGT CTGCCTGGCT 
GTGGCTGCGG CCGTCATGTG CAGGAACAGG AGCACTAAGG GCAAGGATAC TCCAAAGGGC 
TCTGGAAGCA CTGAGCCCAT GATGTCCCCG CAGAGCCACT ACAACGACAG CTCGGAGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766450.2
CDS	272..9754
Translation	MASRARRTAKFSSFQPILAQSPRLLLLLLLLSLVSYVSTQAAGPGAALQSLGLSGTSGVPTEEAIVVANRGLRVPFGREVWLDPLRDLVLQVQPGDRCTVTVLDNDALAQRPGHLSPKRFACDYGPGEVRYSHLGARSPSRDRVRLQLRYDAPGGAIVLPLALEVEVVFTQLEIVTRNLPLVVEELLGTSNALDDRSLEFAYQPETEECRVGILSGLSALPRYGELLHYPQVQGGAGDRGTSKTLLMDCKAFQELGVRYRHTAPSRSPNRDWLPMVVELHSRGAPEGSPALKREHFQVLVRIRGGAENTAPKPSFVAMMMMEVDQFVLTALTPDMLAAEDAESDPDLLIFNLTSAFQPGQGYLVSTDDRSLPLSSFTQRDLRLLKIAYQPPSEDSDQERLFELELEIVDPEGAASDPFAFMVVVKPMNTLAPVVTRNTGLILYEGQYRPLTGPIGSGPQNLVISDEDDLEAVRLEVVAGLRHGHLVILGSPSSDSAPKTFTVAELAAGQVVYQHDDKDGSLSDNLVLRMSDGGGRHQVQFLFPITLVPVDDQPPVLNANTGLTVAEGETVPIPPLTLSATDIDSDDSQLVFVLLPPFSSLGHLLLRQRHVPQEEQGLWQKQGSFYERTVTEWRQQDITEGKLFYRHSGPHSPGPVMDQFMFRVQDNHDPPNQSGIQRFVIRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKRWLHYTDLDTDDRELQYTVTQPPTDTDENHSPAPLGTLVFTDNPSVVVSHFTQAQVNHHKIAYRPPGQELGVAARVAQFQFQVEDRAGNVAPGTFTLYLQPVDNQPPEIVNTGFTVEEKGHHILRETELHVSDVDTDVTHISFTLTQAPKHGHMQISGRPLHVGGQFHLEDIKHGRISYWNSGDESLTDSCSLEVSDRHHVVPITLRVNVRPGDREGPMSVLPAGTLESYLDVLENGATEVTANIIKGAYQGTDDLMLTFLLEGPPSYGEILVNGAPAEQFTQRDILEGSVVYAHTSGEIGLLPKADSFNLSLSAMSQEWRIGSSIVQGVTVWVTILPVDSQAPEISLGEQFVVLEGDKSVISLTHLSAEDMDSLKDDLLCTIVIQPTSGYVENISPAPGSEKSRAGVAISAFTLKDLRQGHINYVQSVHRGVEPVEDRFIFRCSDGINFSERQIFPIVIIPTNDEQPEMFMREFMVMEGMSLVVNRLILNAADADIPRDDLTFTITRFPTHGHVMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGKHSVEKMVLIVVIPVDDETPRMTINNGLEIEIGETKVINNKVLMATDLDSDDKSLVYIIRYGPGHGLLQRQKPLGAFENITLGMNFTQDEVDRNLIQYVHFGQEGIRDLIKFDVTDGTNALIDRYFYVTIGSVDIVFPDVVSKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLECTDRRGLSITSFTQLQLAGNKIYYIHTAEDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHNLVVSESESKLITPFELTVEDRDTPDRLLKFIVTQVPVHGHLLFNNTRSVMVFTKQDLNENLISYKHDGTESTEDSFSFTVTDGTHSDFYVFPDTVFETRRPQVMKIQVLPVDNSVPQIVVNKGASTLRTLATGHLGFMITSKILKVEDRDSLHFSLRFIVTEAPQHGYLLNLGQGNHSVTQFTQADIDDMKICYVLRERANATSDMFHFIVEDDGGNRLTNQHFRLNWAWISFEKEYYLINEDSKFLDIVLTRRGYLGETSFISIGTRDGTAEKDRDFKGKAQKQVQFNPGQTRASWRVRILSDGEHEHSETFQVVLSEPVLAILEFPTVTTVEIIDPGDESTVFIPQSEYSVEEDVGELFIPIRRSGDISRELMVICYTQQGTATSTVRTSVLSYSDYISRPEDHSSVIRFDKDEREKMCRILVIDDSLYEEEETFQVLLSMPMGGRIGDKFPGANVTILTDRDDEPAFYFGDTQYSVDESAGYVELQVWRTGTDLSKPSSVTVRSRKTESLSADAGTDYVGISRNLDFAPGVNMQTVRVVILDDLGRPILEGIEKFELVLRMPMNAALGEPSKATVSINDSASDLPKMQFKERVYTCNENDGRVVAMIYRSGDIQHRSSVRCYTRQGSAQVMMDFEERPNTDVSTVTFLPGEMEKPCVLELMDDAVYEDVEELRLVLGTPQGSSAFGAAVGEQNETLIKIQDEADKAVIKFGETKFSVTEPSRPGESVVVKIPVIRQGDTSKVSIVRVHTKDGSATSGEDYHPVSEEIEFKEGETQHTVEIEVIFDGVREMREAFTVHLKPDENMVAETQATKAIVYIEEIHSMADVTFPSVPHIVSLLIYDDPSKGREDTGPVSGYPVVCITACNPKYPDYEKTGSICASENINDTLTRYRWLISAPAGPDGVTSPMREVDFDTFFTSSKMITLDSIYFQPGSRVQCAARAVNTNGNEGLELMSPIVTIGREEGLCQPRVPGVVGAEPFSAKLRYTGPEDPDFANLIKLTVTMPHIDGMLPAISTRELSNFELTLSPDGTRVGNHKCSNLLDYNEVKTHHGFLTNATKNPEVIGETYPYQYSVPVRGSSTLRFYRNLNLEACLWEFVSYYDMTELLADCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSPVGVGGWQHFDLKSELRLTFVYDTAILWNHGIGSPPEAELQGSLYPTSMRIGEEGRLAVNFKTEAQFHGLFVLSHPASFTSSLIVSADHPGLTFSLRLIRSEPTYNQPVQQWSFVSDFAVRDYSGTYTVKLVPCTTPSNQEYRLPVTCNPREPVTFDLDIRFQQVSDPVATEFSLNTHMYLLSKKNLWLSDGSMGFGQESDVAFAEGDVIYGRVMVDPVQNLGDSFYCSIEKVFLCTGDDGYVPKYSPANAEYGCLADSPSLLHRFKIVDKAQPETQATSFGDVLFNAKLAVDDPEAVLLVNQPGSDGFKVDSTPLFQVALGREWYIHTIYTVKSKDNTHRGIGKRSLEYQYHSVVHPGPPQATTKSWKKRAVRSTPSLAGEIGAENNRGTNIQHISLNRRGKRQVPHGRIPPDGILPWELNSPSSEVSLVTVLGGLTVGLLTVCLAVAAAVMCRNRSTKGKDTPKGSGSTEPMMSPQSHYNDSSEV

Target ORF information:

RefSeq Version	NM_172862.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Fras1 related extracellular matrix protein 2 (Frem2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172862.3

ORF Insert Sequence:

ATGGCCAGCC GTGCGCGCAG GACAGCCAAG TTCTCCAGCT TCCAGCCGAT ACTCGCACAG 
TCGCCTAGGC TGCTGCTGCT GCTGCTGCTC CTGTCACTGG TAAGCTACGT CTCCACACAG 
GCTGCTGGCC CCGGTGCAGC ACTTCAGTCC CTGGGTCTCT CCGGGACTTC GGGAGTCCCT 
ACAGAGGAAG CTATAGTGGT GGCAAACCGC GGGCTCCGTG TGCCCTTCGG TCGCGAAGTC 
TGGCTGGATC CGTTGCGTGA CTTGGTGCTG CAGGTGCAGC CAGGAGACCG CTGCACAGTG 
ACCGTGTTAG ACAACGACGC GCTGGCCCAG CGGCCGGGCC ACTTGAGTCC CAAGCGTTTT 
GCGTGCGACT ATGGCCCGGG TGAGGTGCGC TACTCGCACC TGGGTGCTCG CAGCCCTTCC 
CGCGACCGCG TCCGCCTGCA GCTGCGCTAC GATGCTCCAG GAGGGGCGAT AGTCCTGCCA 
CTGGCACTAG AGGTGGAAGT GGTCTTCACC CAGCTGGAGA TTGTGACTCG GAATTTACCT 
CTGGTCGTGG AAGAACTGCT AGGGACCAGT AATGCCTTGG ACGATCGGAG CTTAGAGTTC 
GCCTACCAGC CTGAGACCGA GGAGTGCCGC GTGGGCATCT TGTCAGGTCT GAGCGCTTTG 
CCTCGTTACG GAGAACTCCT TCACTACCCA CAGGTCCAGG GAGGGGCCGG AGATAGAGGG 
ACCTCTAAGA CCCTTTTGAT GGACTGCAAA GCATTCCAGG AGCTGGGAGT CCGCTACCGC 
CACACAGCCC CCAGCCGCTC GCCTAACAGG GACTGGCTGC CTATGGTGGT CGAGCTGCAT 
TCGCGAGGGG CTCCAGAGGG CAGCCCGGCT CTAAAACGCG AACACTTCCA GGTGCTTGTG 
AGGATTCGAG GAGGAGCGGA GAACACTGCA CCCAAGCCCA GTTTCGTGGC CATGATGATG 
ATGGAGGTGG ACCAGTTTGT ACTGACTGCT CTCACTCCAG ACATGCTAGC AGCCGAGGAT 
GCTGAATCCG ACCCAGACCT TTTGATTTTC AACCTCACCT CTGCCTTCCA GCCTGGGCAG 
GGCTACCTGG TGAGCACGGA TGACAGAAGC CTGCCTCTCT CCTCCTTTAC ACAGCGGGAT 
TTGCGCCTGC TGAAGATTGC CTACCAGCCT CCCTCTGAAG ACTCGGATCA GGAGCGTCTG 
TTTGAACTAG AGCTGGAGAT AGTGGACCCT GAAGGAGCAG CCTCAGACCC TTTCGCCTTC 
ATGGTGGTGG TGAAACCCAT GAACACACTG GCTCCCGTGG TCACACGAAA CACTGGCCTT 
ATTCTCTATG AGGGTCAATA TCGGCCGCTC ACGGGTCCCA TAGGCAGTGG ACCCCAGAAC 
TTGGTCATCA GTGATGAAGA TGACCTAGAA GCTGTGAGGC TGGAGGTGGT GGCTGGGCTC 
CGGCATGGCC ACCTTGTTAT TCTGGGTTCT CCCAGTAGCG ACTCTGCTCC CAAGACCTTC 
ACAGTGGCGG AGCTGGCGGC TGGCCAGGTT GTGTATCAGC ATGATGACAA AGACGGTTCA 
CTGAGTGACA ACTTGGTGCT GCGCATGTCC GATGGAGGAG GCAGGCACCA GGTCCAGTTC 
CTGTTCCCTA TCACTTTAGT GCCTGTGGAT GACCAGCCGC CGGTCCTGAA CGCCAACACT 
GGGCTGACAG TGGCTGAGGG GGAAACTGTG CCTATCCCAC CCTTGACTCT CAGTGCAACC 
GATATAGATT CAGATGACTC TCAGCTGGTT TTTGTCCTGT TGCCACCCTT CTCAAGCTTG 
GGGCACCTGC TTCTCCGCCA AAGGCATGTC CCCCAGGAAG AGCAGGGTCT GTGGCAGAAG 
CAAGGGTCAT TTTACGAGCG AACGGTGACA GAGTGGCGGC AACAGGATAT AACAGAGGGG 
AAGCTCTTCT ATAGGCACTC GGGGCCCCAC AGCCCTGGGC CGGTGATGGA CCAGTTCATG 
TTTAGAGTCC AAGACAATCA TGATCCCCCT AACCAGTCTG GGATCCAGAG GTTTGTAATA 
CGCATTCATC CCGTGGACCG TCTCCCTCCA GAACTGGGCA GTGGCTGTCC CCTTCGGATG 
GTGGTACAGG AATCCCAGCT CACACCACTG AGGAAAAGGT GGCTGCACTA CACTGACCTG 
GACACAGATG ACCGAGAGTT ACAGTACACA GTGACCCAGC CTCCCACGGA CACAGATGAG 
AACCACTCGC CGGCTCCACT GGGCACGTTG GTCTTCACTG ATAATCCCTC AGTGGTGGTG 
AGCCATTTTA CCCAAGCCCA GGTCAATCAT CATAAAATTG CTTACAGGCC CCCAGGGCAG 
GAGCTAGGAG TCGCAGCCCG AGTGGCTCAG TTCCAGTTCC AAGTAGAGGA TCGAGCTGGG 
AATGTGGCTC CTGGCACCTT CACCCTTTAC CTGCAGCCTG TGGATAACCA GCCCCCCGAG 
ATTGTCAACA CTGGCTTCAC CGTTGAGGAG AAGGGCCACC ACATCCTGCG TGAGACCGAG 
CTGCATGTGA GTGATGTTGA CACGGATGTG ACCCACATCT CGTTCACTCT CACACAGGCC 
CCCAAACACG GCCACATGCA AATATCTGGA CGGCCACTGC ATGTAGGCGG ACAATTCCAC 
TTGGAGGACA TAAAGCATGG CAGAATTTCC TACTGGAATA GCGGGGATGA GTCTTTGACT 
GACAGCTGCT CCCTGGAGGT CAGCGACAGA CACCATGTGG TGCCAATCAC TCTCAGAGTG 
AACGTTCGGC CAGGGGACCG TGAAGGTCCT ATGTCAGTCC TTCCTGCTGG TACTCTGGAG 
TCCTATCTAG ACGTCTTAGA GAATGGGGCT ACTGAAGTCA CTGCCAATAT CATTAAGGGG 
GCCTATCAGG GTACCGATGA TTTGATGCTG ACCTTCCTGT TAGAAGGCCC ACCCTCCTAT 
GGGGAAATCT TAGTCAACGG AGCTCCAGCT GAGCAGTTCA CCCAGAGGGA CATCCTGGAG 
GGCTCTGTTG TCTACGCTCA CACCAGTGGT GAGATTGGCC TGTTGCCCAA AGCTGACTCT 
TTTAACCTGA GTCTATCAGC TATGTCTCAA GAATGGAGAA TCGGTAGCAG CATCGTTCAA 
GGCGTCACTG TGTGGGTGAC CATCCTCCCC GTGGACAGCC AAGCCCCTGA GATTTCTCTT 
GGTGAACAGT TTGTAGTGCT GGAAGGTGAC AAGAGTGTGA TAAGCCTGAC CCACCTAAGT 
GCTGAGGACA TGGACTCCCT GAAGGATGAC CTCTTGTGCA CAATAGTCAT TCAGCCAACC 
TCAGGCTATG TAGAGAACAT TTCTCCAGCA CCAGGCTCAG AGAAATCAAG AGCCGGGGTT 
GCCATAAGTG CCTTCACGCT GAAGGATCTC AGGCAAGGAC ACATTAACTA TGTCCAGAGT 
GTCCACAGAG GAGTGGAGCC TGTCGAAGAC CGCTTTATAT TCCGTTGTTC TGACGGTATT 
AACTTCTCTG AGAGACAGAT ATTCCCCATT GTCATCATTC CTACCAATGA CGAGCAGCCA 
GAAATGTTCA TGAGGGAATT CATGGTGATG GAAGGCATGA GTCTGGTGGT CAACAGACTC 
ATCCTCAACG CTGCTGATGC CGACATTCCC AGGGATGATC TAACGTTTAC TATTACCCGG 
TTTCCAACTC ACGGTCATGT CATGAACCAG TTAATCAATG GTACAGTGTT GGTTGAAAGC 
TTCACCCTGG ATCAGATTAT AGAGAGTTCC AGCATTATTT ATGAGCACGA TGACTCTGAG 
ACACAAGAGG ACAGTTTTGT GATTAAACTG ACAGATGGCA AGCACTCCGT GGAGAAGATG 
GTCCTCATTG TGGTCATCCC TGTTGATGAC GAGACACCCA GAATGACCAT CAATAATGGG 
CTAGAAATAG AAATTGGGGA AACCAAAGTT ATCAACAACA AAGTCCTAAT GGCAACCGAT 
CTAGACTCTG ATGACAAATC ACTGGTTTAT ATTATCCGTT ATGGACCAGG GCATGGCTTG 
TTACAGAGAC AAAAACCTCT AGGTGCCTTT GAAAATATCA CTCTAGGCAT GAATTTTACC 
CAGGACGAAG TGGACAGGAA CTTGATTCAA TATGTCCACT TTGGGCAAGA AGGTATTCGA 
GACCTGATTA AATTCGATGT GACAGATGGA ACAAATGCTC TTATAGATCG CTACTTTTAC 
GTGACTATTG GAAGTGTTGA CATTGTCTTC CCTGATGTGG TAAGTAAGGG CGTGTCCTTG 
AAAGAAGGTG GCAAAGTCAC TCTGACAACG GATCTGCTGA GCACAAGTGA CTTGAACAGC 
CCTGATGAGA ACTTAGTTTT CACCATTACC AGGGCTCCTA TGAGAGGTCA CTTGGAGTGC 
ACGGATCGAA GGGGACTGTC CATCACTTCT TTCACTCAGC TCCAGCTGGC TGGTAACAAA 
ATCTACTACA TACACACGGC TGAGGATGAG GTCAAAATGG ACAGCTTCGA GTTTCAAGTC 
ACCGATGGGC GGAACCCTGT CTTCCGAACT TTCCGGATCT CAATCAGTGA CGTAGACAAC 
AAAAAGCCAG TGGTCACCAT CCACAACCTG GTGGTCAGTG AAAGTGAAAG TAAGCTGATC 
ACCCCCTTTG AACTCACTGT GGAGGACAGA GATACCCCTG ACAGGCTCCT AAAATTCATT 
GTCACCCAGG TCCCTGTTCA CGGTCACCTC CTCTTCAACA ATACCAGATC TGTCATGGTT 
TTCACCAAGC AAGACTTGAA TGAAAACTTA ATCAGCTACA AACACGATGG CACAGAATCC 
ACGGAAGACA GCTTCTCCTT CACCGTGACT GACGGCACCC ACTCGGACTT CTATGTTTTT 
CCTGATACAG TGTTTGAAAC CAGGAGACCT CAAGTGATGA AAATCCAGGT GTTACCTGTT 
GACAACAGTG TCCCCCAAAT TGTGGTGAAC AAAGGAGCTT CTACCCTTCG TACCCTGGCC 
ACTGGCCATT TAGGGTTCAT GATCACAAGC AAAATACTCA AAGTGGAGGA CAGAGACAGC 
CTGCATTTTT CTCTCAGGTT CATTGTGACA GAAGCCCCCC AACATGGCTA CCTTCTCAAC 
CTGGGCCAAG GAAACCACAG TGTCACACAG TTCACCCAAG CTGACATTGA TGACATGAAA 
ATATGCTATG TCTTGAGAGA GAGAGCAAAT GCCACAAGTG ATATGTTCCA TTTCATAGTG 
GAAGATGACG GTGGAAACAG GTTAACCAAC CAGCATTTTC GACTGAATTG GGCGTGGATC 
TCCTTTGAAA AAGAGTATTA CTTGATCAAC GAAGACTCCA AATTTCTAGA TATTGTTCTT 
ACACGCAGAG GATACTTGGG AGAAACTTCA TTTATAAGTA TCGGCACCAG GGATGGGACT 
GCGGAAAAAG ACAGAGACTT CAAGGGTAAA GCTCAGAAAC AGGTACAGTT CAACCCCGGC 
CAGACCAGGG CCTCGTGGAG AGTGCGCATC CTGAGCGACG GGGAACACGA GCACTCGGAG 
ACCTTCCAAG TGGTTCTCTC GGAGCCTGTG CTGGCAATCC TGGAGTTCCC CACAGTGACT 
ACAGTGGAGA TCATCGACCC AGGAGACGAA TCAACCGTGT TCATTCCCCA GTCTGAGTAC 
TCTGTTGAAG AAGATGTGGG TGAGCTGTTC ATTCCCATCA GGAGAAGTGG AGACATCAGC 
CGGGAGCTGA TGGTGATCTG CTACACACAG CAAGGGACAG CCACCAGCAC TGTGCGGACG 
TCCGTGCTCT CTTACTCTGA CTACATCTCC AGGCCCGAGG ACCACAGCAG CGTGATCCGC 
TTTGACAAAG ACGAACGAGA GAAAATGTGT CGTATTTTGG TAATTGATGA CTCTTTGTAC 
GAAGAGGAGG AGACTTTCCA GGTCCTTTTG AGCATGCCCA TGGGCGGGAG GATCGGTGAC 
AAGTTTCCAG GAGCCAATGT GACCATCCTC ACAGACAGGG ATGATGAGCC TGCCTTCTAC 
TTTGGGGACA CCCAGTACTC CGTGGATGAG AGTGCCGGCT ACGTGGAGCT GCAGGTGTGG 
AGGACGGGGA CCGACCTCTC TAAGCCTTCC AGTGTCACAG TGAGGTCCCG GAAAACAGAA 
TCCCTCTCTG CAGATGCTGG AACAGACTAT GTGGGAATCA GTCGCAATTT AGATTTTGCG 
CCTGGCGTCA ACATGCAGAC TGTTCGAGTG GTCATTCTGG ATGACCTTGG ACGACCAATA 
CTGGAGGGAA TTGAGAAGTT TGAACTGGTA CTTCGAATGC CTATGAACGC TGCCCTTGGG 
GAGCCCAGCA AGGCCACCGT GTCCATAAAT GACTCTGCCT CAGACTTGCC TAAAATGCAA 
TTCAAAGAGA GAGTGTACAC TTGCAACGAG AATGACGGGC GTGTAGTGGC CATGATCTAC 
AGGAGCGGTG ACATCCAGCA CAGGTCTTCT GTGAGATGTT ACACGAGGCA GGGGTCTGCG 
CAGGTGATGA TGGACTTCGA GGAACGCCCA AATACCGATG TTTCCACTGT CACATTCCTC 
CCTGGTGAGA TGGAGAAGCC GTGTGTCCTC GAGCTGATGG ATGATGCTGT CTATGAGGAT 
GTAGAGGAGC TGCGCCTGGT ACTTGGCACG CCTCAGGGCA GCTCTGCCTT TGGAGCTGCA 
GTGGGCGAGC AGAACGAAAC CCTGATAAAG ATCCAGGATG AGGCTGACAA AGCTGTTATC 
AAATTTGGAG AAACCAAATT TAGTGTCACT GAGCCCAGCA GACCAGGAGA GTCGGTGGTT 
GTCAAAATCC CGGTCATCCG TCAAGGAGAC ACTTCAAAGG TCTCCATCGT GAGAGTCCAC 
ACCAAGGATG GCTCGGCTAC CTCCGGAGAG GATTATCACC CTGTGTCAGA AGAAATCGAG 
TTTAAGGAGG GAGAAACCCA ACACACTGTT GAAATTGAAG TAATCTTCGA CGGGGTGAGA 
GAGATGAGGG AGGCCTTCAC TGTTCACCTA AAACCAGATG AAAATATGGT GGCAGAGACA 
CAGGCAACCA AGGCCATTGT ATATATAGAA GAAATCCACA GCATGGCAGA TGTCACCTTT 
CCTTCTGTCC CTCACATTGT GTCCTTGCTG ATATACGATG ATCCCTCCAA AGGCAGAGAG 
GACACTGGAC CTGTGTCTGG CTACCCGGTT GTCTGCATTA CGGCCTGCAA CCCCAAATAT 
CCAGACTATG AGAAAACAGG CTCTATTTGT GCCAGTGAGA ACATCAACGA CACTTTGACC 
CGATACAGAT GGCTGATCAG CGCTCCCGCT GGCCCTGACG GTGTGACCAG CCCCATGAGA 
GAGGTCGACT TCGACACCTT CTTCACATCA TCCAAAATGA TTACATTAGA CTCCATATAC 
TTTCAGCCTG GCTCCCGGGT CCAATGTGCG GCCCGAGCAG TGAACACCAA TGGGAATGAA 
GGCCTGGAAC TAATGAGCCC CATCGTTACC ATCGGCAGAG AGGAAGGTCT CTGTCAGCCC 
CGTGTGCCTG GGGTAGTGGG GGCAGAGCCA TTCTCCGCTA AGTTGCGCTA CACTGGCCCA 
GAGGACCCAG ACTTTGCCAA CCTCATCAAA CTCACTGTCA CTATGCCGCA CATAGACGGC 
ATGCTCCCTG CCATCTCCAC AAGAGAGCTG TCCAACTTTG AGCTGACCCT CAGCCCTGAC 
GGCACGAGAG TTGGGAATCA CAAATGCTCC AACCTGCTGG ACTACAATGA AGTAAAGACC 
CACCATGGCT TCCTGACCAA CGCCACAAAG AACCCAGAGG TCATTGGAGA GACCTATCCT 
TACCAGTACA GTGTGCCGGT CAGGGGTTCC AGCACCTTAC GCTTCTACCG AAACCTGAAC 
CTGGAAGCAT GTCTGTGGGA GTTTGTCAGC TACTATGACA TGACGGAGCT CCTGGCTGAC 
TGTGGAGGAA CCATCGGGAC CGATGGCCAG GTCCTCAACC TCGTGCAGTC CTACGTGACC 
CTTCGAGTTC CTCTGTACGT TTCCTACGTG TTCCACTCCC CCGTGGGCGT GGGCGGCTGG 
CAGCATTTCG ACTTGAAGTC AGAGCTCCGA CTCACATTCG TGTACGACAC TGCCATCTTG 
TGGAACCATG GGATCGGCAG CCCCCCGGAA GCTGAGCTGC AAGGCTCTCT TTACCCAACC 
AGCATGCGCA TTGGTGAGGA GGGACGCCTG GCTGTGAACT TCAAAACAGA GGCTCAGTTC 
CATGGCTTGT TTGTGCTGTC CCATCCTGCT TCCTTTACCA GCTCACTGAT CGTGTCAGCT 
GACCATCCGG GCCTCACATT TTCCCTCCGC CTTATACGGA GTGAACCAAC CTACAATCAG 
CCAGTCCAGC AGTGGAGTTT CGTGTCAGAC TTTGCGGTCC GTGACTACTC GGGCACCTAC 
ACAGTAAAGT TGGTACCCTG CACCACTCCA TCCAACCAGG AGTACCGTCT GCCAGTCACC 
TGCAACCCCA GAGAGCCTGT CACCTTTGAC CTTGACATTC GATTCCAACA GGTCAGCGAT 
CCCGTGGCGA CTGAGTTCAG CCTGAACACC CACATGTATC TGCTGTCTAA GAAGAATCTC 
TGGTTGTCTG ATGGGTCCAT GGGATTTGGG CAGGAAAGCG ACGTGGCTTT TGCAGAAGGG 
GATGTGATTT ATGGCCGTGT CATGGTCGAT CCTGTCCAGA ACCTCGGTGA CTCCTTTTAC 
TGCAGCATTG AGAAGGTGTT CTTATGCACG GGGGATGACG GCTACGTGCC CAAGTACAGC 
CCAGCGAATG CGGAATATGG CTGCCTGGCT GATTCTCCTT CACTCTTACA TAGATTCAAA 
ATCGTGGATA AGGCTCAGCC AGAGACACAA GCCACCAGCT TTGGAGATGT CCTGTTTAAC 
GCCAAACTGG CAGTGGATGA CCCTGAAGCT GTTCTCTTAG TGAATCAGCC TGGATCCGAT 
GGTTTTAAAG TAGACTCAAC ACCACTCTTT CAGGTTGCCT TGGGCCGAGA GTGGTACATA 
CACACAATCT ATACGGTAAA GTCTAAGGAC AACACCCATC GAGGCATTGG CAAGAGGAGC 
CTGGAGTACC AGTATCACTC AGTGGTGCAT CCCGGACCAC CGCAGGCAAC CACCAAGAGC 
TGGAAGAAGA GAGCAGTCAG GAGCACGCCC TCCCTCGCAG GGGAGATCGG TGCTGAGAAC 
AACCGGGGAA CAAACATCCA ACATATCTCC CTGAACCGCC GTGGCAAGAG GCAGGTCCCC 
CACGGAAGAA TTCCTCCTGA CGGCATCCTG CCCTGGGAGC TCAATAGTCC CAGCTCTGAA 
GTGAGCCTTG TCACTGTCCT GGGAGGCCTC ACGGTGGGCT TGCTCACAGT CTGCCTGGCT 
GTGGCTGCGG CCGTCATGTG CAGGAACAGG AGCACTAAGG GCAAGGATAC TCCAAAGGGC 
TCTGGAAGCA CTGAGCCCAT GATGTCCCCG CAGAGCCACT ACAACGACAG CTCGGAGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Development (Cambridge, England)144(17)3177-3188(2017 09)
Rutledge EA,Benazet JD,McMahon AP

PDGFR? regulates craniofacial development through homodimers and functional heterodimers with PDGFR?.
Genes & development30(21)2443-2458(2016 Nov)
Fantauzzo KA,Soriano P

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Frem2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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