Twf2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Twf2
Entrez Gene ID	23999
Full Name	twinfilin actin binding protein 2
Synonyms	A6-related,AU014993,Ptk9l,Ptk9r
General protein information	Preferred Names twinfilin actin binding protein 2 Names twinfilin-2 PTK9 protein typrotein tyrosine kinase 9-like (A6-related protein) mA6RP protein tyrosine kinase 9 related protein protein tyrosine kinase 9-like (A6-related protein) twinfilin, actin-binding protein, homolog 2 twinfilin-1-like protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_011876.3	NP_036006.1	twinfilin-2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TWF2	NP_009215.1
Gallus gallus (chicken)	TWF2	NP_001025760.1
Danio rerio (zebrafish)	twf2	NP_001009897.1
Pan troglodytes (chimpanzee)	TWF2	XP_003309871.1
Mus musculus (house mouse)	Twf2	NP_036006.1
Rattus norvegicus (Norway rat)	Twf2	NP_001128710.1
Xenopus tropicalis (tropical clawed frog)	twf2	NP_001123417.1
Bos taurus (cattle)	TWF2	NP_001098794.1

Gene Ontology
Bibliography

Related articles in PubMed

Twinfilin 2a regulates platelet reactivity and turnover in mice.
Stritt S, Beck S, Becker IC, V?gtle T, Hakala M, Heinze KG, Du X, Bender M, Braun A, Lappalainen P, Nieswandt B
Blood130(15)1746-1756(2017 10)

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Li Y, Klena NT, Gabriel GC, Liu X, Kim AJ, Lemke K, Chen Y, Chatterjee B, Devine W, Damerla RR, Chang C, Yagi H, San Agustin JT, Thahir M, Anderton S, Lawhead C, Vescovi A, Pratt H, Morgan J, Haynes L, Smith CL, Eppig JT, Reinholdt L, Francis R, Leatherbury L, Ganapathiraju MK, Tobita K, Pazour GJ, Lo CW
Nature521(7553)520-4(2015 May)

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
Tang F, Barbacioru C, Nordman E, Bao S, Lee C, Wang X, Tuch BB, Heard E, Lao K, Surani MA
PloS one6(6)e21208(2011)

Twinfilin-2a is dispensable for mouse development.
Nevalainen EM, Braun A, Vartiainen MK, Serlachius M, Andersson LC, Moser M, Lappalainen P
PloS one6(8)e22894(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Twf2a-controlled actin rearrangements dampen platelet activation responses.
Title: Twinfilin 2a regulates platelet reactivity and turnover in mice.

These data suggest that twinfilin-2a plays a redundant role in cytoskeletal dynamics with the biochemically similar twinfilin-1, which is typically co-expressed in same tissues with twinfilin-2a.
Title: Twinfilin-2a is dispensable for mouse development.

This study suggested that twinfilin 2 plays a role in the regulation of stereocilia elongation by restricting excessive elongation of the shorter row stereocilia thereby maintaining the mature staircase architecture of cochlear hair bundles.
Title: Twinfilin 2 regulates actin filament lengths in cochlear stereocilia.

MyosinVIIa interacts with Twinfilin-2 at the tips of mechanosensory stereocilia in the inner ear
Title: MyosinVIIa interacts with Twinfilin-2 at the tips of mechanosensory stereocilia in the inner ear.

like Twf1, mouse Twf2 is a filament barbed-end capping protein, and that two tissue-specific and biochemically distinct isoforms are generated from the Twf2 gene through alternative promoter usage
Title: Two biochemically distinct and tissue-specific twinfilin isoforms are generated from the mouse Twf2 gene by alternative promoter usage.

The following Twf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Twf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu09059	NM_011876.3 Latest version!	Mus musculus twinfilin actin binding protein 2 (Twf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Twf2 ( NM_011876.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036006.1 Mus musculus twinfilin actin binding protein 2 (Twf2), mRNA.

Price & Availability↑

CloneID	OMu09059
Clone ID Related Accession (Same CDS sequence)	NM_011876.3
Accession Version	NM_011876.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	twinfilin-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC164430.3. On Mar 21, 2007 this sequence version replaced NM_011876.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK084026.1, AK166900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCACC AGACCGGCAT CCACGCCACT GAAGAGCTAA AGGAATTCTT TGCCAAGGCC 
CGGGCTGGCT CCATCCGACT TATCAAAGTC ATCATTGAGG ACGAACAGCT CGTGCTGGGT 
GCCTCACAGG AGCCAGTGGG ACGCTGGGAC CAGGACTACG ACCGGGCTGT GCTGCCACTG 
CTAGACGCCC AAGAGCCCTG CTACCTCCTC TTCCGACTTG ATTCGCAGAA TGCTCAGGGT 
TTCGAGTGGC TTTTCCTGGC CTGGTCACCT GACAATTCGC CGGTGCGGCT GAAGATGCTA 
TATGCAGCCA CACGCGCCAC CGTGAAGAAG GAGTTTGGAG GCGGCCACAT CAAGGATGAG 
CTCTTCGGGA CAGTAAAGGA TGACCTCTCC TTGGCTGGGT ACCAGAAGCA CCTGTCATCG 
TGTGCCGCAC CTGCCCCACT GACTTCTGCC GAGAGAGAAC TCCAGCAGAT CCGAATCAAT 
GAGGTGAAGA CTGAGATCAG CGTGGAAAGT AAACACCAGA CGCTGCAGGG CCTGGCCTTC 
CCTCTGCAGC CTGAGGCCCA GAGGGCACTT CAGCAACTCA AACAGAAGAC GGTCAACTAT 
ATCCAGCTGA AGCTGGACCT GGAACGGGAG ACCATCGAGC TGGTACACAC AGAACCCACA 
AATGTGGCCC AGCTGCCCTC ACGGATTCCT CGAGATGCTG CCCGCTACCA CTTCTTCCTA 
TACAAGCATA CTCATGAGGG TGATGCCCTT GAATCTGTGG TGTTCATCTA CTCCATGCCC 
GGGTACAAGT GCAGCATCAA GGAGCGGATG CTCTACTCCA GCTGCAAGAG CCGCCTCCTT 
GACTCCGTAG AGCAAGACTT CCAGCTGGAG ATAGCTAAGA AGATTGAGAT CGGCGATGGA 
GCAGAGCTGA CAGCTGAGTT CCTCTATGAC GAGGTACATC CCAAGCAACA CGCGTTCAAG 
CAGGCGTTCG CCAAGCCCAA AGGCCCCGGG GGCAAGCGGG GCCACAAGCG CCTCATCCGG 
GGCCCCGGGG AGAACGGGGA AGACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036006.1
CDS	153..1202
Translation	MAHQTGIHATEELKEFFAKARAGSIRLIKVIIEDEQLVLGASQEPVGRWDQDYDRAVLPLLDAQEPCYLLFRLDSQNAQGFEWLFLAWSPDNSPVRLKMLYAATRATVKKEFGGGHIKDELFGTVKDDLSLAGYQKHLSSCAAPAPLTSAERELQQIRINEVKTEISVESKHQTLQGLAFPLQPEAQRALQQLKQKTVNYIQLKLDLERETIELVHTEPTNVAQLPSRIPRDAARYHFFLYKHTHEGDALESVVFIYSMPGYKCSIKERMLYSSCKSRLLDSVEQDFQLEIAKKIEIGDGAELTAEFLYDEVHPKQHAFKQAFAKPKGPGGKRGHKRLIRGPGENGEDS

Target ORF information:

RefSeq Version	NM_011876.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus twinfilin actin binding protein 2 (Twf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011876.3

ORF Insert Sequence:

ATGGCGCACC AGACCGGCAT CCACGCCACT GAAGAGCTAA AGGAATTCTT TGCCAAGGCC 
CGGGCTGGCT CCATCCGACT TATCAAAGTC ATCATTGAGG ACGAACAGCT CGTGCTGGGT 
GCCTCACAGG AGCCAGTGGG ACGCTGGGAC CAGGACTACG ACCGGGCTGT GCTGCCACTG 
CTAGACGCCC AAGAGCCCTG CTACCTCCTC TTCCGACTTG ATTCGCAGAA TGCTCAGGGT 
TTCGAGTGGC TTTTCCTGGC CTGGTCACCT GACAATTCGC CGGTGCGGCT GAAGATGCTA 
TATGCAGCCA CACGCGCCAC CGTGAAGAAG GAGTTTGGAG GCGGCCACAT CAAGGATGAG 
CTCTTCGGGA CAGTAAAGGA TGACCTCTCC TTGGCTGGGT ACCAGAAGCA CCTGTCATCG 
TGTGCCGCAC CTGCCCCACT GACTTCTGCC GAGAGAGAAC TCCAGCAGAT CCGAATCAAT 
GAGGTGAAGA CTGAGATCAG CGTGGAAAGT AAACACCAGA CGCTGCAGGG CCTGGCCTTC 
CCTCTGCAGC CTGAGGCCCA GAGGGCACTT CAGCAACTCA AACAGAAGAC GGTCAACTAT 
ATCCAGCTGA AGCTGGACCT GGAACGGGAG ACCATCGAGC TGGTACACAC AGAACCCACA 
AATGTGGCCC AGCTGCCCTC ACGGATTCCT CGAGATGCTG CCCGCTACCA CTTCTTCCTA 
TACAAGCATA CTCATGAGGG TGATGCCCTT GAATCTGTGG TGTTCATCTA CTCCATGCCC 
GGGTACAAGT GCAGCATCAA GGAGCGGATG CTCTACTCCA GCTGCAAGAG CCGCCTCCTT 
GACTCCGTAG AGCAAGACTT CCAGCTGGAG ATAGCTAAGA AGATTGAGAT CGGCGATGGA 
GCAGAGCTGA CAGCTGAGTT CCTCTATGAC GAGGTACATC CCAAGCAACA CGCGTTCAAG 
CAGGCGTTCG CCAAGCCCAA AGGCCCCGGG GGCAAGCGGG GCCACAAGCG CCTCATCCGG 
GGCCCCGGGG AGAACGGGGA AGACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Twinfilin 2a regulates platelet reactivity and turnover in mice.
Blood130(15)1746-1756(2017 10)
Stritt S,Beck S,Becker IC,V?gtle T,Hakala M,Heinze KG,Du X,Bender M,Braun A,Lappalainen P,Nieswandt B

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Nature521(7553)520-4(2015 May)
Li Y,Klena NT,Gabriel GC,Liu X,Kim AJ,Lemke K,Chen Y,Chatterjee B,Devine W,Damerla RR,Chang C,Yagi H,San Agustin JT,Thahir M,Anderton S,Lawhead C,Vescovi A,Pratt H,Morgan J,Haynes L,Smith CL,Eppig JT,Reinholdt L,Francis R,Leatherbury L,Ganapathiraju MK,Tobita K,Pazour GJ,Lo CW

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
PloS one6(6)e21208(2011)
Tang F,Barbacioru C,Nordman E,Bao S,Lee C,Wang X,Tuch BB,Heard E,Lao K,Surani MA

Twinfilin-2a is dispensable for mouse development.
PloS one6(8)e22894(2011)
Nevalainen EM,Braun A,Vartiainen MK,Serlachius M,Andersson LC,Moser M,Lappalainen P

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Twf2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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