Oas1b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Oas1b
Entrez Gene ID	23961
Full Name	2'-5' oligoadenylate synthetase 1B
Synonyms	Flv,L1,Mmu-L1,Oas1,Oias-2,Oias2,Wnv
General protein information	Preferred Names 2'-5' oligoadenylate synthetase 1B Names inactive 2'-5'-oligoadenylate synthase 1B (2-5')oligo(A) synthase 1B (2-5')oligo(A) synthetase 1b 2'-5'-oligoadenylate synthase-like protein 1 2'-5'-oligoadenylate synthetase-like protein 1 2-5A synthase 1B 2-5A synthetase 1B Flavivirus resistance
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 F\|5 60.64 cM
Summary	This gene is a member of the 2'-5' oligoA synthetase family, which are clustered on chromosome 5. The encoded protein functions in the interferon-regulated OAS/RNase L system, which mediates RNA decay as part of the innate antiviral immunity pathway. The protein binds double-stranded RNA and oligomerizes ATP, which activate the single-stranded RNA cleavage enzyme RNase L. This protein mediates resistance to flaviviruses such as West Nile virus. The majority of wild mouse strains produce a functional protein and are resistant to flavivirus infection, whereas some inbred mouse strains including the strain of the reference genome, C57BL/6J, contain a premature stop codon that inactivates this gene product. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001083925.1	NP_001077394.1	inactive 2'-5'-oligoadenylate synthase 1B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Oas1b-dependent Immune Transcriptional Profiles of West Nile Virus Infection in the Collaborative Cross.
Green R, Wilkins C, Thomas S, Sekine A, Hendrick DM, Voss K, Ireton RC, Mooney M, Go JT, Choonoo G, Jeng S, de Villena FP, Ferris MT, McWeeney S, Gale M Jr
G3 (Bethesda, Md.)7(6)1665-1682(2017 Jun)

Susceptibility to flavivirus-specific antiviral response of Oas1b affects the neurovirulence of the Far-Eastern subtype of tick-borne encephalitis virus.
Yoshii K, Moritoh K, Nagata N, Yokozawa K, Sakai M, Sasaki N, Kariwa H, Agui T, Takashima I
Archives of virology158(5)1039-46(2013 May)

Identification of novel host cell binding partners of Oas1b, the protein conferring resistance to flavivirus-induced disease in mice.
Courtney SC, Di H, Stockman BM, Liu H, Scherbik SV, Brinton MA
Journal of virology86(15)7953-63(2012 Aug)

Activation of Oas1a gene expression by type I IFN requires both STAT1 and STAT2 while only STAT2 is required for Oas1b activation.
Pulit-Penaloza JA, Scherbik SV, Brinton MA
Virology425(2)71-81(2012 Apr)

The oligoadenylate synthetase family: an ancient protein family with multiple antiviral activities.
Kristiansen H, Gad HH, Eskildsen-Larsen S, Despres P, Hartmann R
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research31(1)41-7(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data indicate that the different susceptibility to the antiviral activity of Oas1b resulted in a difference in neurovirulence in the two tick-borne encephalitis virus strains.
Title: Susceptibility to flavivirus-specific antiviral response of Oas1b affects the neurovirulence of the Far-Eastern subtype of tick-borne encephalitis virus.

Oas1b is part of a complex located in the ER and that ABCF3 is a component of the Flv(r)-mediated resistance mechanism.
Title: Identification of novel host cell binding partners of Oas1b, the protein conferring resistance to flavivirus-induced disease in mice.

Activation of Oas1b gene expression by type I IFN requires only STAT2, not STAT1.
Title: Activation of Oas1a gene expression by type I IFN requires both STAT1 and STAT2 while only STAT2 is required for Oas1b activation.

Oas1b inhibits flavivirus replication by an alternative mechanism that overrides the proviral effect of reducing 2-5A accumulation and RNase L activation.
Title: The Flvr-encoded murine oligoadenylate synthetase 1b (Oas1b) suppresses 2-5A synthesis in intact cells.

Oas1b mice show resistance to the infection of West Nile virus but not of influenza virus.
Title: Generation of congenic mouse strains by introducing the virus-resistant genes, Mx1 and Oas1b, of feral mouse-derived inbred strain MSM/Ms into the common strain C57BL/6J.

The following Oas1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Oas1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02863	NM_001083925.1 Latest version!	Mus musculus 2'-5' oligoadenylate synthetase 1B (Oas1b), transcript variant 1, coding, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Oas1b ( NM_001083925.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001077394.1 Mus musculus 2'-5' oligoadenylate synthetase 1B (Oas1b), transcript variant 1, coding, mRNA.

Price & Availability↑

CloneID	OMu02863
Clone ID Related Accession (Same CDS sequence)	NM_001083925.1
Accession Version	NM_001083925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	inactive 2'-5'-oligoadenylate synthase 1B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF418005.1. Transcript Variant: This variant (1, coding) represents the functional allele that is not present in some strains, including the strain of the reference genome. Sequence Note: This RefSeq record was created to represent the transcript and the full-length, functional protein that is expressed in several strains. It does not contain a premature stop codon that truncates the coding region of several strains, including the strain of the reference genome, C57BL/6J. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF418004.1, AF418005.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## polymorphic pseudogene :: PMID: 12186974 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGG ATCTGAGGAG CATCCCGGCC TCGAAGCTTG ATAAGTTCAT AGAGAACCAT 
CTCCCGGACA CCAGCTTCTG TGCTGACCTC AGAGAAGTCA TAGATGCCCT GTGTGCTCTC 
CTGAAGGACA GATCCTTCCG GGGCCCCGTC CGCCGAATGA GGGCCTCTAA AGGGGTCAAG 
GGCAAAGGCA CCACACTCAA GGGCAGGTCA GACGCTGACC TGGTGGTGTT CCTTAACAAT 
CTCACCAGCT TTGAGGATCA GTTAAACCAA CAGGGAGTGT TGATTAAGGA AATTAAGAAA 
CAGCTGTGCG AGGTTCAGCA TGAGAGACGT TGTGGAGTGA AGTTTGAGGT CCACAGTTTA 
AGGAGTCCCA ACTCCCGGGC TCTGAGCTTC AAGCTGAGCG CCCCCGACCT GCTGAAGGAG 
GTGAAGTTTG ATGTGCTGCC AGCCTATGAT TTACTGGATC ATCTTAACAT CCTCAAGAAG 
CCTAACCAAC AATTCTACGC CAATCTCATC AGTGGGCGTA CCCCGCCGGG GAAGGAGGGC 
AAGTTATCGA TCTGCTTTAT GGGGCTTCGG AAGTACTTCC TGAACTGTCG CCCAACCAAG 
CTGAAGCGCC TCATCCGCCT GGTCACGCAC TGGTACCAAC TGTGTAAGGA GAAGCTGGGG 
GACCCGCTGC CCCCACAGTA TGCCCTGGAG CTGCTCACAG TCTATGCCTG GGAGTATGGG 
AGTCGAGTAA CTAAATTCAA CACAGCCCAG GGCTTCCGAA CCGTCTTGGA ACTGGTCACC 
AAGTACAAAC AGCTTCGAAT CTACTGGACA GTGTATTATG ACTTTCGACA TCAAGAGGTC 
TCTGAATACC TGCACCAACA GCTCAAAAAA GACAGGCCTG TGATCTTGGA CCCCGCTGAC 
CCAACAAGGA ACATAGCTGG TTTGAACCCA AAGGACTGGC GGCGTCTAGC AGGAGAGGCT 
GCCACCTGGC TGCAATACCC ATGCTTTAAG TACAGGGACG GTTCCCCAGT GTGCTCCTGG 
GAGGTGCCGA CGGAGGTTGG AGTGCCAATG AAGTATCTCT TTTGTCGTAT TTTCTGGTTA 
TTGTTTTGGT CTTTGTTTCA TTTCATCTTT GGGAAGACTT CATCTGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077394.1
CDS	64..1194
Translation	MEQDLRSIPASKLDKFIENHLPDTSFCADLREVIDALCALLKDRSFRGPVRRMRASKGVKGKGTTLKGRSDADLVVFLNNLTSFEDQLNQQGVLIKEIKKQLCEVQHERRCGVKFEVHSLRSPNSRALSFKLSAPDLLKEVKFDVLPAYDLLDHLNILKKPNQQFYANLISGRTPPGKEGKLSICFMGLRKYFLNCRPTKLKRLIRLVTHWYQLCKEKLGDPLPPQYALELLTVYAWEYGSRVTKFNTAQGFRTVLELVTKYKQLRIYWTVYYDFRHQEVSEYLHQQLKKDRPVILDPADPTRNIAGLNPKDWRRLAGEAATWLQYPCFKYRDGSPVCSWEVPTEVGVPMKYLFCRIFWLLFWSLFHFIFGKTSSG

Target ORF information:

RefSeq Version	NM_001083925.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus 2'-5' oligoadenylate synthetase 1B (Oas1b), transcript variant 1, coding, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083925.1

ORF Insert Sequence:

ATGGAGCAGG ATCTGAGGAG CATCCCGGCC TCGAAGCTTG ATAAGTTCAT AGAGAACCAT 
CTCCCGGACA CCAGCTTCTG TGCTGACCTC AGAGAAGTCA TAGATGCCCT GTGTGCTCTC 
CTGAAGGACA GATCCTTCCG GGGCCCCGTC CGCCGAATGA GGGCCTCTAA AGGGGTCAAG 
GGCAAAGGCA CCACACTCAA GGGCAGGTCA GACGCTGACC TGGTGGTGTT CCTTAACAAT 
CTCACCAGCT TTGAGGATCA GTTAAACCAA CAGGGAGTGT TGATTAAGGA AATTAAGAAA 
CAGCTGTGCG AGGTTCAGCA TGAGAGACGT TGTGGAGTGA AGTTTGAGGT CCACAGTTTA 
AGGAGTCCCA ACTCCCGGGC TCTGAGCTTC AAGCTGAGCG CCCCCGACCT GCTGAAGGAG 
GTGAAGTTTG ATGTGCTGCC AGCCTATGAT TTACTGGATC ATCTTAACAT CCTCAAGAAG 
CCTAACCAAC AATTCTACGC CAATCTCATC AGTGGGCGTA CCCCGCCGGG GAAGGAGGGC 
AAGTTATCGA TCTGCTTTAT GGGGCTTCGG AAGTACTTCC TGAACTGTCG CCCAACCAAG 
CTGAAGCGCC TCATCCGCCT GGTCACGCAC TGGTACCAAC TGTGTAAGGA GAAGCTGGGG 
GACCCGCTGC CCCCACAGTA TGCCCTGGAG CTGCTCACAG TCTATGCCTG GGAGTATGGG 
AGTCGAGTAA CTAAATTCAA CACAGCCCAG GGCTTCCGAA CCGTCTTGGA ACTGGTCACC 
AAGTACAAAC AGCTTCGAAT CTACTGGACA GTGTATTATG ACTTTCGACA TCAAGAGGTC 
TCTGAATACC TGCACCAACA GCTCAAAAAA GACAGGCCTG TGATCTTGGA CCCCGCTGAC 
CCAACAAGGA ACATAGCTGG TTTGAACCCA AAGGACTGGC GGCGTCTAGC AGGAGAGGCT 
GCCACCTGGC TGCAATACCC ATGCTTTAAG TACAGGGACG GTTCCCCAGT GTGCTCCTGG 
GAGGTGCCGA CGGAGGTTGG AGTGCCAATG AAGTATCTCT TTTGTCGTAT TTTCTGGTTA 
TTGTTTTGGT CTTTGTTTCA TTTCATCTTT GGGAAGACTT CATCTGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Oas1b-dependent Immune Transcriptional Profiles of West Nile Virus Infection in the Collaborative Cross.
G3 (Bethesda, Md.)7(6)1665-1682(2017 Jun)
Green R,Wilkins C,Thomas S,Sekine A,Hendrick DM,Voss K,Ireton RC,Mooney M,Go JT,Choonoo G,Jeng S,de Villena FP,Ferris MT,McWeeney S,Gale M Jr

Susceptibility to flavivirus-specific antiviral response of Oas1b affects the neurovirulence of the Far-Eastern subtype of tick-borne encephalitis virus.
Archives of virology158(5)1039-46(2013 May)
Yoshii K,Moritoh K,Nagata N,Yokozawa K,Sakai M,Sasaki N,Kariwa H,Agui T,Takashima I

Identification of novel host cell binding partners of Oas1b, the protein conferring resistance to flavivirus-induced disease in mice.
Journal of virology86(15)7953-63(2012 Aug)
Courtney SC,Di H,Stockman BM,Liu H,Scherbik SV,Brinton MA

Activation of Oas1a gene expression by type I IFN requires both STAT1 and STAT2 while only STAT2 is required for Oas1b activation.
Virology425(2)71-81(2012 Apr)
Pulit-Penaloza JA,Scherbik SV,Brinton MA

The oligoadenylate synthetase family: an ancient protein family with multiple antiviral activities.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research31(1)41-7(2011 Jan)
Kristiansen H,Gad HH,Eskildsen-Larsen S,Despres P,Hartmann R

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Oas1b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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