Mtr cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mtr
Entrez Gene ID	238505
Full Name	5-methyltetrahydrofolate-homocysteine methyltransferase
Synonyms	AI894170,D830038K18Rik,MS
General protein information	Preferred Names 5-methyltetrahydrofolate-homocysteine methyltransferase Names methionine synthase vitamin-B12 dependent methionine synthase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 A1\|13 4.52 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001081128.3	NP_001074597.1	methionine synthase
XM_006516661.3	XP_006516724.1	methionine synthase isoform X1
XM_006516662.3	XP_006516725.1	methionine synthase isoform X2
XM_006516661.4	XP_006516724.1	methionine synthase isoform X1

Pathways from BioSystems

Homologies

	MTR	XP_001109238.1
Rattus norvegicus (Norway rat)	Mtr	NP_110491.1
Danio rerio (zebrafish)	mtr	NP_932338.1
Xenopus tropicalis (tropical clawed frog)	LOC100488827	XP_002933914.1
Homo sapiens (human)	MTR	NP_000245.2
Canis lupus familiaris (dog)	MTR	XP_005618839.1
Caenorhabditis elegans (roundworm)	metr-1	NP_496353.1
Pan troglodytes (chimpanzee)	MTR	XP_514294.2
Mus musculus (house mouse)	Mtr	NP_001074597.1
Gallus gallus (chicken)	MTR	NP_001026275.1
Bos taurus (cattle)	MTR	NP_001025469.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of Hematopoiesis and Methionine Homeostasis by mTORC1 Inhibitor NPRL2.
Dutchak PA, Laxman S, Estill SJ, Wang C, Wang Y, Wang Y, Bulut GB, Gao J, Huang LJ, Tu BP
Cell reports12(3)371-9(2015 Jul)

Spatial and temporal expression of folate-related transporters and metabolic enzymes during mouse placental development.
Cherukad J, Wainwright V, Watson ED
Placenta33(5)440-8(2012 May)

Regulation of homocysteine homeostasis through the transcriptional coactivator PGC-1alpha.
Li S, Arning E, Liu C, Vitvitsky V, Hernandez C, Banerjee R, Bottiglieri T, Lin JD
American journal of physiology. Endocrinology and metabolism296(3)E543-8(2009 Mar)

Glycine N-methyltransferase-/- mice develop chronic hepatitis and glycogen storage disease in the liver.
Liu SP, Li YS, Chen YJ, Chiang EP, Li AF, Lee YH, Tsai TF, Hsiao M, Huang SF, Hwang SF, Chen YM
Hepatology (Baltimore, Md.)46(5)1413-25(2007 Nov)

Genetic and nutritional deficiencies in folate metabolism influence tumorigenicity in Apcmin/+ mice.
Lawrance AK, Deng L, Brody LC, Finnell RH, Shane B, Rozen R
The Journal of nutritional biochemistry18(5)305-12(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Deficiency of either MS or folate produces oxidative stress and endothelial dysfunction in the cerebral microcirculation of mice.
Title: Cerebral vascular dysfunction in methionine synthase-deficient mice.

The following Mtr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mtr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu48383	XM_006516662.3 Latest version!	Mus musculus 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu48382	XM_006516661.4 Latest version!	Mus musculus 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu01747	NM_001081128.3 Latest version!	Mus musculus 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$734.30 $1049.00
View Old Accession Versions >>
OMu48382	XM_006516661.3	Mus musculus 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mtr ( XM_006516661.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516724.1 Mus musculus 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu48382
Clone ID Related Accession (Same CDS sequence)	XM_006516661.3 , XM_006516661.4
Accession Version	XM_006516661.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methionine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516661.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCTT TCATTGAAAC AATTGGAAAA TGTACAACAG CCTATGTCCT CTGTTACCCA 
AATGCAGGTC TTCCCAACAC TTTTGGCGAC TATGATGAAA CACCATCCAC GATGGCCACG 
CACCTGAAGG ACTTTGCTGT GGATGGCTTG GTGAATATAG TTGGTGGATG CTGTGGTTCA 
ACACCTGATC ACATCAGGGA AATTGCGGAA GCTGTGAAAA AGTGTAAGCC AAGAGTCCCC 
CCTGCTAGTG TTTTTGAAGG ACATATGTTA CTGTCTGGTC TAGAGCCCTT CAGGATTGGA 
CCATACACCA ACTTTGTTAA CATTGGAGAG CGCTGTAATG TGGCAGGATC CAGGAAATTT 
GCTAAACTCA TCATGGCAGG AAACTATGAA GAAGCCCTGA GCATTGCCAA AGCACAGGTG 
GAAATGGGAG CTCAGGTGCT CGATATCAAC ATGGACGACG GCATGCTGGA TGGTCCCAGT 
GCAATGACCA GATTTTGCAA CTCCATTGCT TCTGAGCCTG ACATTGCCAA GGTGCCCCTG 
TGCATTGACT CTTCTAACTT TGCCGTGATT GAAGCTGGGT TGAAGTGCTG CCAAGGGAAG 
TGCATAGTCA ACAGCATCAG CCTCAAGGAG GGGGAGGGGG ACTTCCTGGA GAAGGCCCGG 
AAGATTAAGA AGTTTGGAGC TGCTGTGGTG GTTATGGCTT TTGATGAGGA AGGACAAGCA 
ACAGAAACAG ATGTTAAAGT CAATGTGTGC ACCAGAGCTT ACCATCTACT TGTGGACAAA 
GTGGGCTTCA ATCCAAATGA CATCATCTTT GACCCCAACA TCCTTACCAT TGGTACTGGA 
ATGGAGGAAC ATAACTTGTA TGCTATCAAT TTTATCCATG CAACAAGAGT CATTAAGGAA 
ACCTTACCAG GAGTCAGAAT AAGTGGAGGT CTTTCCAACT TATCCTTCTC CTTCCGAGGG 
ATGGAAGCCA TTCGAGAAGC AATGCATGGT GTGTTCCTTT ATCACGCGAT CAAGTTTGGT 
ATGGACATGG GGATAGTCAA TGCCGGCAAC CTCCCTGTGT ATGATGCTAT CCACAAGGAC 
CTTCTTCAGC TCTGTGAAGA CCTCATCTGG AACAAAGACT CTGAGGCTAC TGAGAAACTC 
TTGCGATATG CCCAGACTCA TGGCACAGGA GGGAAGAAAG TCATCCAGAC AGATGAGTGG 
AGGAATGGCT CCATTGAAGA GCGCCTGGAG TATGCTCTTG TGAAGGGCAT TGAAAAGCAC 
ATTGTTGAAG ATACTGAAGA AGCCAGGTTA AACGGGGAAA AATACCCTCG GCCTCTGAAT 
ATAATTGAAG GGCCCCTAAT GAATGGCATG AAAGTGGTTG GTGATCTTTT TGGAGCTGGA 
AAGATGTTTC TACCTCAGGT TATAAAGTCA GCCCGTGTCA TGAAGAAGGC TGTTGGCCAC 
CTTATCCCTT TCATGGAAAA AGAAAGAGAA GAAGCCAGAT TGATTAATGG TTCGGTTGAA 
GAAGAGGACC CTTACCAAGG CACCATTGTG CTGGCCACTG TTAAAGGGGA CGTGCATGAC 
ATCGGCAAGA ACATAGTTGG CGTGGTGCTG GCCTGTAACA ATTTCAGAGT TATTGATTTA 
GGAGTCATGA CTCCCTGTGA TAAGATACTT CAAGCTGCTC TGGACCACAA AGCAGATATA 
ATTGGCTTGT CAGGACTCAT CACTCCATCC TTGGATGAGA TGATTTTTGT TGCCAAGGAA 
ATGGAGAGGT TGGCTATAAA GATTCCATTG TTGATAGGAG GAGCAACCAC TTCAAGAACC 
CACACAGCAG TTAAAATTGC ACCACGGTAC AGCGCGCCTG TGATCCACGT CCTAGATGCA 
TCCAAGAGTG TGGTGGTGTG TTCTCAGCTG TTAGATGAAA ATCTGAGAGA TGACTACTTT 
GAAGAAATAC TGGAAGAGTA TGAAGATATT AGACAGGACC ACTATGAGTC TCTCAAAGAG 
AGAAAATACG TACCCCTAAG TCAAGCCAGA AAACACGGTT TCCACATTGA TTGGCTCTCT 
GAACCTCATC CAGTGAAGCC CACGTTTATT GGGACCCAGG TCTTTGAGGA CTACAACCTG 
CAAAAGCTGG TGGACTACAT TGACTGGAAG CCTTTCTTTG ACGTCTGGCA GCTCCGGGGC 
AAGTACCCGA ACCGAGGATT TCCCAAGATA TTTAATGACA AAGCAGTAGG TGAAGAAGCC 
AGAAAGGTAT ACAATGATGC TCAGAATATG CTGAACATAC TGATTAGTCA AAAGAAACTG 
CAGGCCAGGG GTGTGGTTGG ATTCTGGCCA GCACAGAGTG TCCAGGATGA CATCCACCTG 
TATGCAGAGG GGGTGGTGCC CCAGGCCGCT GAGCCCATAG CCACCTTCTA TGGACTGAGG 
CAGCAGGCTG AGAAGGACTC TTCTAGTACA GACCCCTACC ACTGCCTCTC AGACTTCATT 
GCTCCTTTGC ATTCTGGTGT CTGTGACTAC TTGGGTCTGT TTGCTGTTGC CTGCTTTGGG 
GTTGAAGAGC TGAGTAAGAC CTATGAGGAT GATGGCGATG ACTACAGCAG CATCATGGTG 
AAGGCGCTGG GCGACAGGCT GGCAGAGGCC TTTGCAGAGG AGCTCCATGA GAGAGTTCGC 
CGAGAACTGT GGGCCTACTC TAGGAGTGAG CAGTTGGGCG TCCCAGACTT GCGCAGACTC 
CGGTATGAGG GCATCCGGCC AGCTCCTGGT TACCCCAGCC AGCCTGACCA TACTGAGAAG 
CTTACCATGT GGAGACTGGC CAGCATCGAG CAGGCCACAG GCATCAGGCT GACAGAATCC 
TTGGCAATGG CACCCGCTTC AGCAGTGTCA GGGCTGTACT TCTCCAACGT AAAGGCCAAA 
TATTTTGCTG TGGGGAAAAT TTCCAAAGAT CAGACTGAGG ATTATGCTTT GAGGAAGAAC 
ATGCCAGTGG CCGAGGTGGA GAAATGGCTT GGTCCCATTC TGGGCTATGA CACAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516724.1
CDS	1028..4027
Translation	MRPFIETIGKCTTAYVLCYPNAGLPNTFGDYDETPSTMATHLKDFAVDGLVNIVGGCCGSTPDHIREIAEAVKKCKPRVPPASVFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRKFAKLIMAGNYEEALSIAKAQVEMGAQVLDINMDDGMLDGPSAMTRFCNSIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEGDFLEKARKIKKFGAAVVVMAFDEEGQATETDVKVNVCTRAYHLLVDKVGFNPNDIIFDPNILTIGTGMEEHNLYAINFIHATRVIKETLPGVRISGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGNLPVYDAIHKDLLQLCEDLIWNKDSEATEKLLRYAQTHGTGGKKVIQTDEWRNGSIEERLEYALVKGIEKHIVEDTEEARLNGEKYPRPLNIIEGPLMNGMKVVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREEARLINGSVEEEDPYQGTIVLATVKGDVHDIGKNIVGVVLACNNFRVIDLGVMTPCDKILQAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLRDDYFEEILEEYEDIRQDHYESLKERKYVPLSQARKHGFHIDWLSEPHPVKPTFIGTQVFEDYNLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFNDKAVGEEARKVYNDAQNMLNILISQKKLQARGVVGFWPAQSVQDDIHLYAEGVVPQAAEPIATFYGLRQQAEKDSSSTDPYHCLSDFIAPLHSGVCDYLGLFAVACFGVEELSKTYEDDGDDYSSIMVKALGDRLAEAFAEELHERVRRELWAYSRSEQLGVPDLRRLRYEGIRPAPGYPSQPDHTEKLTMWRLASIEQATGIRLTESLAMAPASAVSGLYFSNVKAKYFAVGKISKDQTEDYALRKNMPVAEVEKWLGPILGYDTD

Target ORF information:

RefSeq Version	XM_006516661.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516661.3

ORF Insert Sequence:

ATGAGGCCTT TCATTGAAAC AATTGGAAAA TGTACAACAG CCTATGTCCT CTGTTACCCA 
AATGCAGGTC TTCCCAACAC TTTTGGCGAC TATGATGAAA CACCATCCAC GATGGCCACG 
CACCTGAAGG ACTTTGCTGT GGATGGCTTG GTGAATATAG TTGGTGGATG CTGTGGTTCA 
ACACCTGATC ACATCAGGGA AATTGCGGAA GCTGTGAAAA AGTGTAAGCC AAGAGTCCCC 
CCTGCTAGTG TTTTTGAAGG ACATATGTTA CTGTCTGGTC TAGAGCCCTT CAGGATTGGA 
CCATACACCA ACTTTGTTAA CATTGGAGAG CGCTGTAATG TGGCAGGATC CAGGAAATTT 
GCTAAACTCA TCATGGCAGG AAACTATGAA GAAGCCCTGA GCATTGCCAA AGCACAGGTG 
GAAATGGGAG CTCAGGTGCT CGATATCAAC ATGGACGACG GCATGCTGGA TGGTCCCAGT 
GCAATGACCA GATTTTGCAA CTCCATTGCT TCTGAGCCTG ACATTGCCAA GGTGCCCCTG 
TGCATTGACT CTTCTAACTT TGCCGTGATT GAAGCTGGGT TGAAGTGCTG CCAAGGGAAG 
TGCATAGTCA ACAGCATCAG CCTCAAGGAG GGGGAGGGGG ACTTCCTGGA GAAGGCCCGG 
AAGATTAAGA AGTTTGGAGC TGCTGTGGTG GTTATGGCTT TTGATGAGGA AGGACAAGCA 
ACAGAAACAG ATGTTAAAGT CAATGTGTGC ACCAGAGCTT ACCATCTACT TGTGGACAAA 
GTGGGCTTCA ATCCAAATGA CATCATCTTT GACCCCAACA TCCTTACCAT TGGTACTGGA 
ATGGAGGAAC ATAACTTGTA TGCTATCAAT TTTATCCATG CAACAAGAGT CATTAAGGAA 
ACCTTACCAG GAGTCAGAAT AAGTGGAGGT CTTTCCAACT TATCCTTCTC CTTCCGAGGG 
ATGGAAGCCA TTCGAGAAGC AATGCATGGT GTGTTCCTTT ATCACGCGAT CAAGTTTGGT 
ATGGACATGG GGATAGTCAA TGCCGGCAAC CTCCCTGTGT ATGATGCTAT CCACAAGGAC 
CTTCTTCAGC TCTGTGAAGA CCTCATCTGG AACAAAGACT CTGAGGCTAC TGAGAAACTC 
TTGCGATATG CCCAGACTCA TGGCACAGGA GGGAAGAAAG TCATCCAGAC AGATGAGTGG 
AGGAATGGCT CCATTGAAGA GCGCCTGGAG TATGCTCTTG TGAAGGGCAT TGAAAAGCAC 
ATTGTTGAAG ATACTGAAGA AGCCAGGTTA AACGGGGAAA AATACCCTCG GCCTCTGAAT 
ATAATTGAAG GGCCCCTAAT GAATGGCATG AAAGTGGTTG GTGATCTTTT TGGAGCTGGA 
AAGATGTTTC TACCTCAGGT TATAAAGTCA GCCCGTGTCA TGAAGAAGGC TGTTGGCCAC 
CTTATCCCTT TCATGGAAAA AGAAAGAGAA GAAGCCAGAT TGATTAATGG TTCGGTTGAA 
GAAGAGGACC CTTACCAAGG CACCATTGTG CTGGCCACTG TTAAAGGGGA CGTGCATGAC 
ATCGGCAAGA ACATAGTTGG CGTGGTGCTG GCCTGTAACA ATTTCAGAGT TATTGATTTA 
GGAGTCATGA CTCCCTGTGA TAAGATACTT CAAGCTGCTC TGGACCACAA AGCAGATATA 
ATTGGCTTGT CAGGACTCAT CACTCCATCC TTGGATGAGA TGATTTTTGT TGCCAAGGAA 
ATGGAGAGGT TGGCTATAAA GATTCCATTG TTGATAGGAG GAGCAACCAC TTCAAGAACC 
CACACAGCAG TTAAAATTGC ACCACGGTAC AGCGCGCCTG TGATCCACGT CCTAGATGCA 
TCCAAGAGTG TGGTGGTGTG TTCTCAGCTG TTAGATGAAA ATCTGAGAGA TGACTACTTT 
GAAGAAATAC TGGAAGAGTA TGAAGATATT AGACAGGACC ACTATGAGTC TCTCAAAGAG 
AGAAAATACG TACCCCTAAG TCAAGCCAGA AAACACGGTT TCCACATTGA TTGGCTCTCT 
GAACCTCATC CAGTGAAGCC CACGTTTATT GGGACCCAGG TCTTTGAGGA CTACAACCTG 
CAAAAGCTGG TGGACTACAT TGACTGGAAG CCTTTCTTTG ACGTCTGGCA GCTCCGGGGC 
AAGTACCCGA ACCGAGGATT TCCCAAGATA TTTAATGACA AAGCAGTAGG TGAAGAAGCC 
AGAAAGGTAT ACAATGATGC TCAGAATATG CTGAACATAC TGATTAGTCA AAAGAAACTG 
CAGGCCAGGG GTGTGGTTGG ATTCTGGCCA GCACAGAGTG TCCAGGATGA CATCCACCTG 
TATGCAGAGG GGGTGGTGCC CCAGGCCGCT GAGCCCATAG CCACCTTCTA TGGACTGAGG 
CAGCAGGCTG AGAAGGACTC TTCTAGTACA GACCCCTACC ACTGCCTCTC AGACTTCATT 
GCTCCTTTGC ATTCTGGTGT CTGTGACTAC TTGGGTCTGT TTGCTGTTGC CTGCTTTGGG 
GTTGAAGAGC TGAGTAAGAC CTATGAGGAT GATGGCGATG ACTACAGCAG CATCATGGTG 
AAGGCGCTGG GCGACAGGCT GGCAGAGGCC TTTGCAGAGG AGCTCCATGA GAGAGTTCGC 
CGAGAACTGT GGGCCTACTC TAGGAGTGAG CAGTTGGGCG TCCCAGACTT GCGCAGACTC 
CGGTATGAGG GCATCCGGCC AGCTCCTGGT TACCCCAGCC AGCCTGACCA TACTGAGAAG 
CTTACCATGT GGAGACTGGC CAGCATCGAG CAGGCCACAG GCATCAGGCT GACAGAATCC 
TTGGCAATGG CACCCGCTTC AGCAGTGTCA GGGCTGTACT TCTCCAACGT AAAGGCCAAA 
TATTTTGCTG TGGGGAAAAT TTCCAAAGAT CAGACTGAGG ATTATGCTTT GAGGAAGAAC 
ATGCCAGTGG CCGAGGTGGA GAAATGGCTT GGTCCCATTC TGGGCTATGA CACAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mtr ( XM_006516662.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516725.1 Mus musculus 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu48383
Clone ID Related Accession (Same CDS sequence)	XM_006516662.3
Accession Version	XM_006516662.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methionine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516662.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAA CCCTGCAGGA TGAGATTGAA GCCATTCTTC GGAAGAGGAT CATGGTGCTA 
GACGGTGGGA TGGGGACCAT GATCCAGCGG TACAAACTAA GTGAAGAACA TTTCCAAGGT 
CAAGAGTTTA AAGATCACAG CAGGCCACTG AAAGGCAACA ATGACATCTT AAGTATAACT 
CAGCCTGATA TTATTTACCA GATTCATAAG GAATACTTGC TGGCTGGAGC AGATATCATC 
GAAACAAACA CTTTCAGCAG TACTAGTATC GCCCAGGCTG ACTATGGCCT TGAACACTTG 
GCCTACCGGA TGAACAAGTG CTCTGCAGAT GTGGCCAGAA AAGCCGCTGA GGAGATAACT 
CTGCAGACAG GAGTCAAGAG GTTTGTGGCT GGAGCTCTGG GTCCGACTAA TAAGACACTT 
TCTGTCTCCC CATCTGTGGA AAGGCCAGAT TATAGGAACA TCACATTTGA TGAGCTTGTT 
GACGCATACC AGGAGCAGGC CAAGGGACTG CTGGATGGCA GGGTCGACAT CTTACTCATT 
GAAACAATTT TTGATACAGC TAATGCCAAA GCAGCCTTGT TTGCGATCCA GAACCTCTTT 
GAAGAGAATT ATGCTCCTCC TCGGCCTATC TTTATTTCTG GGACCATTGT TGATAAAAGT 
GGAAGGACTC TTTCTGGGCA GACGGGAGAG GCTTTTGTCA CCAGCGTGTC TCATTCGGAC 
CCCCTGTGCA TTGGATTAAA TTGTTCTCTG GGTGCAGCTG AAATGAGGCC TTTCATTGAA 
ACAATTGGAA AATGTACAAC AGCCTATGTC CTCTGTTACC CAAATGCAGG TCTTCCCAAC 
ACTTTTGGCG ACTATGATGA AACACCATCC ACGATGGCCA CGCACCTGAA GGACTTTGCT 
GTGGATGGCT TGGTGAATAT AGTTGGTGGA TGCTGTGGTT CAACACCTGA TCACATCAGG 
GAAATTGCGG AAGCTGTGAA AAAGTGTAAG CCAAGAGTCC CCCCTGCTAG TGTTTTTGAA 
GGACATATGT TACTGTCTGG TCTAGAGCCC TTCAGGATTG GACCATACAC CAACTTTGTT 
AACATTGGAG AGCGCTGTAA TGTGGCAGGA TCCAGGAAAT TTGCTAAACT CATCATGGCA 
GGAAACTATG AAGAAGCCCT GAGCATTGCC AAAGCACAGG TGGAAATGGG AGCTCAGGTG 
CTCGATATCA ACATGGACGA CGGCATGCTG GATGGTCCCA GTGCAATGAC CAGATTTTGC 
AACTCCATTG CTTCTGAGCC TGACATTGCC AAGGTGCCCC TGTGCATTGA CTCTTCTAAC 
TTTGCCGTGA TTGAAGCTGG GTTGAAGTGC TGCCAAGGGA AGTGCATAGT CAACAGCATC 
AGCCTCAAGG AGGGGGAGGG GGACTTCCTG GAGAAGGCCC GGAAGATTAA GAAGTTTGGA 
GCTGCTGTGG TGGTTATGGC TTTTGATGAG GAAGGACAAG CAACAGAAAC AGATGTTAAA 
GTCAATGTGT GCACCAGAGC TTACCATCTA CTTGTGGACA AAGTGGGCTT CAATCCAAAT 
GACATCATCT TTGACCCCAA CATCCTTACC ATTGGTACTG GAATGGAGGA ACATAACTTG 
TATGCTATCA ATTTTATCCA TGCAACAAGA GTCATTAAGG AAACCTTACC AGGAGTCAGA 
ATAAGTGGAG GTCTTTCCAA CTTATCCTTC TCCTTCCGAG GGATGGAAGC CATTCGAGAA 
GCAATGCATG GTGTGTTCCT TTATCACGCG ATCAAGTTTG GTATGGACAT GGGGATAGTC 
AATGCCGGCA ACCTCCCTGT GTATGATGCT ATCCACAAGG ACCTTCTTCA GCTCTGTGAA 
GACCTCATCT GGAACAAAGA CTCTGAGGCT ACTGAGAAAC TCTTGCGATA TGCCCAGACT 
CATGGCACAG GAGGGAAGAA AGTCATCCAG ACAGATGAGT GGAGGAATGG CTCCATTGAA 
GAGCGCCTGG AGTATGCTCT TGTGAAGGGC ATTGAAAAGC ACATTGTTGA AGATACTGAA 
GAAGCCAGGT TAAACGGGGA AAAATACCCT CGGCCTCTGA ATATAATTGA AGGGCCCCTA 
ATGAATGGCA TGAAAGTGGT TGGTGATCTT TTTGGAGCTG GAAAGATGTT TCTACCTCAG 
GTTATAAAGT CAGCCCGTGT CATGAAGAAG GCTGTTGGCC ACCTTATCCC TTTCATGGAA 
AAAGAAAGAG AAGAAGCCAG ATTGATTAAT GGTTCGGTTG AAGAAGAGGA CCCTTACCAA 
GGCACCATTG TGCTGGCCAC TGTTAAAGGG GACGTGCATG ACATCGGCAA GAACATAGTT 
GGCGTGGTGC TGGCCTGTAA CAATTTCAGA GTTATTGATT TAGGAGTCAT GACTCCCTGT 
GATAAGATAC TTCAAGCTGC TCTGGACCAC AAAGCAGATA TAATTGGCTT GTCAGGACTC 
ATCACTCCAT CCTTGGATGA GATGATTTTT GTTGCCAAGG AAATGGAGAG GTTGGCTATA 
AAGATTCCAT TGTTGATAGG AGGAGCAACC ACTTCAAGAA CCCACACAGC AGTTAAAATT 
GCACCACGGT ACAGCGCGCC TGTGATCCAC GTCCTAGATG CATCCAAGAG TGTGGTGGTG 
TGTTCTCAGC TGTTAGATGA AAATCTGAGA GATGACTACT TTGAAGAAAT ACTGGAAGAG 
TATGAAGATA TTAGACAGGA CCACTATGAG TCTCTCAAAG AGAGAAAATA CGTACCCCTA 
AGTCAAGCCA GAAAACACGG TTTCCACATT GATTGGCTCT CTGAACCTCA TCCAGTGAAG 
CCCACGTTTA TTGGGACCCA GGTCTTTGAG GACTACAACC TGCAAAAGCT GGTGGACTAC 
ATTGACTGGA AGCCTTTCTT TGACGTCTGG CAGCTCCGGG GCAAGTACCC GAACCGAGGA 
TTTCCCAAGA TATTTAATGA CAAAGCAGTA GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516725.1
CDS	338..3313
Translation	MKKTLQDEIEAILRKRIMVLDGGMGTMIQRYKLSEEHFQGQEFKDHSRPLKGNNDILSITQPDIIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNKCSADVARKAAEEITLQTGVKRFVAGALGPTNKTLSVSPSVERPDYRNITFDELVDAYQEQAKGLLDGRVDILLIETIFDTANAKAALFAIQNLFEENYAPPRPIFISGTIVDKSGRTLSGQTGEAFVTSVSHSDPLCIGLNCSLGAAEMRPFIETIGKCTTAYVLCYPNAGLPNTFGDYDETPSTMATHLKDFAVDGLVNIVGGCCGSTPDHIREIAEAVKKCKPRVPPASVFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRKFAKLIMAGNYEEALSIAKAQVEMGAQVLDINMDDGMLDGPSAMTRFCNSIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEGDFLEKARKIKKFGAAVVVMAFDEEGQATETDVKVNVCTRAYHLLVDKVGFNPNDIIFDPNILTIGTGMEEHNLYAINFIHATRVIKETLPGVRISGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGNLPVYDAIHKDLLQLCEDLIWNKDSEATEKLLRYAQTHGTGGKKVIQTDEWRNGSIEERLEYALVKGIEKHIVEDTEEARLNGEKYPRPLNIIEGPLMNGMKVVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREEARLINGSVEEEDPYQGTIVLATVKGDVHDIGKNIVGVVLACNNFRVIDLGVMTPCDKILQAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLRDDYFEEILEEYEDIRQDHYESLKERKYVPLSQARKHGFHIDWLSEPHPVKPTFIGTQVFEDYNLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFNDKAVG

Target ORF information:

RefSeq Version	XM_006516662.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516662.3

ORF Insert Sequence:

ATGAAGAAAA CCCTGCAGGA TGAGATTGAA GCCATTCTTC GGAAGAGGAT CATGGTGCTA 
GACGGTGGGA TGGGGACCAT GATCCAGCGG TACAAACTAA GTGAAGAACA TTTCCAAGGT 
CAAGAGTTTA AAGATCACAG CAGGCCACTG AAAGGCAACA ATGACATCTT AAGTATAACT 
CAGCCTGATA TTATTTACCA GATTCATAAG GAATACTTGC TGGCTGGAGC AGATATCATC 
GAAACAAACA CTTTCAGCAG TACTAGTATC GCCCAGGCTG ACTATGGCCT TGAACACTTG 
GCCTACCGGA TGAACAAGTG CTCTGCAGAT GTGGCCAGAA AAGCCGCTGA GGAGATAACT 
CTGCAGACAG GAGTCAAGAG GTTTGTGGCT GGAGCTCTGG GTCCGACTAA TAAGACACTT 
TCTGTCTCCC CATCTGTGGA AAGGCCAGAT TATAGGAACA TCACATTTGA TGAGCTTGTT 
GACGCATACC AGGAGCAGGC CAAGGGACTG CTGGATGGCA GGGTCGACAT CTTACTCATT 
GAAACAATTT TTGATACAGC TAATGCCAAA GCAGCCTTGT TTGCGATCCA GAACCTCTTT 
GAAGAGAATT ATGCTCCTCC TCGGCCTATC TTTATTTCTG GGACCATTGT TGATAAAAGT 
GGAAGGACTC TTTCTGGGCA GACGGGAGAG GCTTTTGTCA CCAGCGTGTC TCATTCGGAC 
CCCCTGTGCA TTGGATTAAA TTGTTCTCTG GGTGCAGCTG AAATGAGGCC TTTCATTGAA 
ACAATTGGAA AATGTACAAC AGCCTATGTC CTCTGTTACC CAAATGCAGG TCTTCCCAAC 
ACTTTTGGCG ACTATGATGA AACACCATCC ACGATGGCCA CGCACCTGAA GGACTTTGCT 
GTGGATGGCT TGGTGAATAT AGTTGGTGGA TGCTGTGGTT CAACACCTGA TCACATCAGG 
GAAATTGCGG AAGCTGTGAA AAAGTGTAAG CCAAGAGTCC CCCCTGCTAG TGTTTTTGAA 
GGACATATGT TACTGTCTGG TCTAGAGCCC TTCAGGATTG GACCATACAC CAACTTTGTT 
AACATTGGAG AGCGCTGTAA TGTGGCAGGA TCCAGGAAAT TTGCTAAACT CATCATGGCA 
GGAAACTATG AAGAAGCCCT GAGCATTGCC AAAGCACAGG TGGAAATGGG AGCTCAGGTG 
CTCGATATCA ACATGGACGA CGGCATGCTG GATGGTCCCA GTGCAATGAC CAGATTTTGC 
AACTCCATTG CTTCTGAGCC TGACATTGCC AAGGTGCCCC TGTGCATTGA CTCTTCTAAC 
TTTGCCGTGA TTGAAGCTGG GTTGAAGTGC TGCCAAGGGA AGTGCATAGT CAACAGCATC 
AGCCTCAAGG AGGGGGAGGG GGACTTCCTG GAGAAGGCCC GGAAGATTAA GAAGTTTGGA 
GCTGCTGTGG TGGTTATGGC TTTTGATGAG GAAGGACAAG CAACAGAAAC AGATGTTAAA 
GTCAATGTGT GCACCAGAGC TTACCATCTA CTTGTGGACA AAGTGGGCTT CAATCCAAAT 
GACATCATCT TTGACCCCAA CATCCTTACC ATTGGTACTG GAATGGAGGA ACATAACTTG 
TATGCTATCA ATTTTATCCA TGCAACAAGA GTCATTAAGG AAACCTTACC AGGAGTCAGA 
ATAAGTGGAG GTCTTTCCAA CTTATCCTTC TCCTTCCGAG GGATGGAAGC CATTCGAGAA 
GCAATGCATG GTGTGTTCCT TTATCACGCG ATCAAGTTTG GTATGGACAT GGGGATAGTC 
AATGCCGGCA ACCTCCCTGT GTATGATGCT ATCCACAAGG ACCTTCTTCA GCTCTGTGAA 
GACCTCATCT GGAACAAAGA CTCTGAGGCT ACTGAGAAAC TCTTGCGATA TGCCCAGACT 
CATGGCACAG GAGGGAAGAA AGTCATCCAG ACAGATGAGT GGAGGAATGG CTCCATTGAA 
GAGCGCCTGG AGTATGCTCT TGTGAAGGGC ATTGAAAAGC ACATTGTTGA AGATACTGAA 
GAAGCCAGGT TAAACGGGGA AAAATACCCT CGGCCTCTGA ATATAATTGA AGGGCCCCTA 
ATGAATGGCA TGAAAGTGGT TGGTGATCTT TTTGGAGCTG GAAAGATGTT TCTACCTCAG 
GTTATAAAGT CAGCCCGTGT CATGAAGAAG GCTGTTGGCC ACCTTATCCC TTTCATGGAA 
AAAGAAAGAG AAGAAGCCAG ATTGATTAAT GGTTCGGTTG AAGAAGAGGA CCCTTACCAA 
GGCACCATTG TGCTGGCCAC TGTTAAAGGG GACGTGCATG ACATCGGCAA GAACATAGTT 
GGCGTGGTGC TGGCCTGTAA CAATTTCAGA GTTATTGATT TAGGAGTCAT GACTCCCTGT 
GATAAGATAC TTCAAGCTGC TCTGGACCAC AAAGCAGATA TAATTGGCTT GTCAGGACTC 
ATCACTCCAT CCTTGGATGA GATGATTTTT GTTGCCAAGG AAATGGAGAG GTTGGCTATA 
AAGATTCCAT TGTTGATAGG AGGAGCAACC ACTTCAAGAA CCCACACAGC AGTTAAAATT 
GCACCACGGT ACAGCGCGCC TGTGATCCAC GTCCTAGATG CATCCAAGAG TGTGGTGGTG 
TGTTCTCAGC TGTTAGATGA AAATCTGAGA GATGACTACT TTGAAGAAAT ACTGGAAGAG 
TATGAAGATA TTAGACAGGA CCACTATGAG TCTCTCAAAG AGAGAAAATA CGTACCCCTA 
AGTCAAGCCA GAAAACACGG TTTCCACATT GATTGGCTCT CTGAACCTCA TCCAGTGAAG 
CCCACGTTTA TTGGGACCCA GGTCTTTGAG GACTACAACC TGCAAAAGCT GGTGGACTAC 
ATTGACTGGA AGCCTTTCTT TGACGTCTGG CAGCTCCGGG GCAAGTACCC GAACCGAGGA 
TTTCCCAAGA TATTTAATGA CAAAGCAGTA GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mtr ( XM_006516661.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516724.1 Mus musculus 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), transcript variant X1, mRNA.

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CloneID	OMu48382
Clone ID Related Accession (Same CDS sequence)	XM_006516661.3 , XM_006516661.4
Accession Version	XM_006516661.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	methionine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516661.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCTT TCATTGAAAC AATTGGAAAA TGTACAACAG CCTATGTCCT CTGTTACCCA 
AATGCAGGTC TTCCCAACAC TTTTGGCGAC TATGATGAAA CACCATCCAC GATGGCCACG 
CACCTGAAGG ACTTTGCTGT GGATGGCTTG GTGAATATAG TTGGTGGATG CTGTGGTTCA 
ACACCTGATC ACATCAGGGA AATTGCGGAA GCTGTGAAAA AGTGTAAGCC AAGAGTCCCC 
CCTGCTAGTG TTTTTGAAGG ACATATGTTA CTGTCTGGTC TAGAGCCCTT CAGGATTGGA 
CCATACACCA ACTTTGTTAA CATTGGAGAG CGCTGTAATG TGGCAGGATC CAGGAAATTT 
GCTAAACTCA TCATGGCAGG AAACTATGAA GAAGCCCTGA GCATTGCCAA AGCACAGGTG 
GAAATGGGAG CTCAGGTGCT CGATATCAAC ATGGACGACG GCATGCTGGA TGGTCCCAGT 
GCAATGACCA GATTTTGCAA CTCCATTGCT TCTGAGCCTG ACATTGCCAA GGTGCCCCTG 
TGCATTGACT CTTCTAACTT TGCCGTGATT GAAGCTGGGT TGAAGTGCTG CCAAGGGAAG 
TGCATAGTCA ACAGCATCAG CCTCAAGGAG GGGGAGGGGG ACTTCCTGGA GAAGGCCCGG 
AAGATTAAGA AGTTTGGAGC TGCTGTGGTG GTTATGGCTT TTGATGAGGA AGGACAAGCA 
ACAGAAACAG ATGTTAAAGT CAATGTGTGC ACCAGAGCTT ACCATCTACT TGTGGACAAA 
GTGGGCTTCA ATCCAAATGA CATCATCTTT GACCCCAACA TCCTTACCAT TGGTACTGGA 
ATGGAGGAAC ATAACTTGTA TGCTATCAAT TTTATCCATG CAACAAGAGT CATTAAGGAA 
ACCTTACCAG GAGTCAGAAT AAGTGGAGGT CTTTCCAACT TATCCTTCTC CTTCCGAGGG 
ATGGAAGCCA TTCGAGAAGC AATGCATGGT GTGTTCCTTT ATCACGCGAT CAAGTTTGGT 
ATGGACATGG GGATAGTCAA TGCCGGCAAC CTCCCTGTGT ATGATGCTAT CCACAAGGAC 
CTTCTTCAGC TCTGTGAAGA CCTCATCTGG AACAAAGACT CTGAGGCTAC TGAGAAACTC 
TTGCGATATG CCCAGACTCA TGGCACAGGA GGGAAGAAAG TCATCCAGAC AGATGAGTGG 
AGGAATGGCT CCATTGAAGA GCGCCTGGAG TATGCTCTTG TGAAGGGCAT TGAAAAGCAC 
ATTGTTGAAG ATACTGAAGA AGCCAGGTTA AACGGGGAAA AATACCCTCG GCCTCTGAAT 
ATAATTGAAG GGCCCCTAAT GAATGGCATG AAAGTGGTTG GTGATCTTTT TGGAGCTGGA 
AAGATGTTTC TACCTCAGGT TATAAAGTCA GCCCGTGTCA TGAAGAAGGC TGTTGGCCAC 
CTTATCCCTT TCATGGAAAA AGAAAGAGAA GAAGCCAGAT TGATTAATGG TTCGGTTGAA 
GAAGAGGACC CTTACCAAGG CACCATTGTG CTGGCCACTG TTAAAGGGGA CGTGCATGAC 
ATCGGCAAGA ACATAGTTGG CGTGGTGCTG GCCTGTAACA ATTTCAGAGT TATTGATTTA 
GGAGTCATGA CTCCCTGTGA TAAGATACTT CAAGCTGCTC TGGACCACAA AGCAGATATA 
ATTGGCTTGT CAGGACTCAT CACTCCATCC TTGGATGAGA TGATTTTTGT TGCCAAGGAA 
ATGGAGAGGT TGGCTATAAA GATTCCATTG TTGATAGGAG GAGCAACCAC TTCAAGAACC 
CACACAGCAG TTAAAATTGC ACCACGGTAC AGCGCGCCTG TGATCCACGT CCTAGATGCA 
TCCAAGAGTG TGGTGGTGTG TTCTCAGCTG TTAGATGAAA ATCTGAGAGA TGACTACTTT 
GAAGAAATAC TGGAAGAGTA TGAAGATATT AGACAGGACC ACTATGAGTC TCTCAAAGAG 
AGAAAATACG TACCCCTAAG TCAAGCCAGA AAACACGGTT TCCACATTGA TTGGCTCTCT 
GAACCTCATC CAGTGAAGCC CACGTTTATT GGGACCCAGG TCTTTGAGGA CTACAACCTG 
CAAAAGCTGG TGGACTACAT TGACTGGAAG CCTTTCTTTG ACGTCTGGCA GCTCCGGGGC 
AAGTACCCGA ACCGAGGATT TCCCAAGATA TTTAATGACA AAGCAGTAGG TGAAGAAGCC 
AGAAAGGTAT ACAATGATGC TCAGAATATG CTGAACATAC TGATTAGTCA AAAGAAACTG 
CAGGCCAGGG GTGTGGTTGG ATTCTGGCCA GCACAGAGTG TCCAGGATGA CATCCACCTG 
TATGCAGAGG GGGTGGTGCC CCAGGCCGCT GAGCCCATAG CCACCTTCTA TGGACTGAGG 
CAGCAGGCTG AGAAGGACTC TTCTAGTACA GACCCCTACC ACTGCCTCTC AGACTTCATT 
GCTCCTTTGC ATTCTGGTGT CTGTGACTAC TTGGGTCTGT TTGCTGTTGC CTGCTTTGGG 
GTTGAAGAGC TGAGTAAGAC CTATGAGGAT GATGGCGATG ACTACAGCAG CATCATGGTG 
AAGGCGCTGG GCGACAGGCT GGCAGAGGCC TTTGCAGAGG AGCTCCATGA GAGAGTTCGC 
CGAGAACTGT GGGCCTACTC TAGGAGTGAG CAGTTGGGCG TCCCAGACTT GCGCAGACTC 
CGGTATGAGG GCATCCGGCC AGCTCCTGGT TACCCCAGCC AGCCTGACCA TACTGAGAAG 
CTTACCATGT GGAGACTGGC CAGCATCGAG CAGGCCACAG GCATCAGGCT GACAGAATCC 
TTGGCAATGG CACCCGCTTC AGCAGTGTCA GGGCTGTACT TCTCCAACGT AAAGGCCAAA 
TATTTTGCTG TGGGGAAAAT TTCCAAAGAT CAGACTGAGG ATTATGCTTT GAGGAAGAAC 
ATGCCAGTGG CCGAGGTGGA GAAATGGCTT GGTCCCATTC TGGGCTATGA CACAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516724.1
CDS	1026..4025
Translation	MRPFIETIGKCTTAYVLCYPNAGLPNTFGDYDETPSTMATHLKDFAVDGLVNIVGGCCGSTPDHIREIAEAVKKCKPRVPPASVFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRKFAKLIMAGNYEEALSIAKAQVEMGAQVLDINMDDGMLDGPSAMTRFCNSIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEGDFLEKARKIKKFGAAVVVMAFDEEGQATETDVKVNVCTRAYHLLVDKVGFNPNDIIFDPNILTIGTGMEEHNLYAINFIHATRVIKETLPGVRISGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGNLPVYDAIHKDLLQLCEDLIWNKDSEATEKLLRYAQTHGTGGKKVIQTDEWRNGSIEERLEYALVKGIEKHIVEDTEEARLNGEKYPRPLNIIEGPLMNGMKVVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREEARLINGSVEEEDPYQGTIVLATVKGDVHDIGKNIVGVVLACNNFRVIDLGVMTPCDKILQAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLRDDYFEEILEEYEDIRQDHYESLKERKYVPLSQARKHGFHIDWLSEPHPVKPTFIGTQVFEDYNLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFNDKAVGEEARKVYNDAQNMLNILISQKKLQARGVVGFWPAQSVQDDIHLYAEGVVPQAAEPIATFYGLRQQAEKDSSSTDPYHCLSDFIAPLHSGVCDYLGLFAVACFGVEELSKTYEDDGDDYSSIMVKALGDRLAEAFAEELHERVRRELWAYSRSEQLGVPDLRRLRYEGIRPAPGYPSQPDHTEKLTMWRLASIEQATGIRLTESLAMAPASAVSGLYFSNVKAKYFAVGKISKDQTEDYALRKNMPVAEVEKWLGPILGYDTD

Target ORF information:

RefSeq Version	XM_006516661.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516661.4

ORF Insert Sequence:

ATGAGGCCTT TCATTGAAAC AATTGGAAAA TGTACAACAG CCTATGTCCT CTGTTACCCA 
AATGCAGGTC TTCCCAACAC TTTTGGCGAC TATGATGAAA CACCATCCAC GATGGCCACG 
CACCTGAAGG ACTTTGCTGT GGATGGCTTG GTGAATATAG TTGGTGGATG CTGTGGTTCA 
ACACCTGATC ACATCAGGGA AATTGCGGAA GCTGTGAAAA AGTGTAAGCC AAGAGTCCCC 
CCTGCTAGTG TTTTTGAAGG ACATATGTTA CTGTCTGGTC TAGAGCCCTT CAGGATTGGA 
CCATACACCA ACTTTGTTAA CATTGGAGAG CGCTGTAATG TGGCAGGATC CAGGAAATTT 
GCTAAACTCA TCATGGCAGG AAACTATGAA GAAGCCCTGA GCATTGCCAA AGCACAGGTG 
GAAATGGGAG CTCAGGTGCT CGATATCAAC ATGGACGACG GCATGCTGGA TGGTCCCAGT 
GCAATGACCA GATTTTGCAA CTCCATTGCT TCTGAGCCTG ACATTGCCAA GGTGCCCCTG 
TGCATTGACT CTTCTAACTT TGCCGTGATT GAAGCTGGGT TGAAGTGCTG CCAAGGGAAG 
TGCATAGTCA ACAGCATCAG CCTCAAGGAG GGGGAGGGGG ACTTCCTGGA GAAGGCCCGG 
AAGATTAAGA AGTTTGGAGC TGCTGTGGTG GTTATGGCTT TTGATGAGGA AGGACAAGCA 
ACAGAAACAG ATGTTAAAGT CAATGTGTGC ACCAGAGCTT ACCATCTACT TGTGGACAAA 
GTGGGCTTCA ATCCAAATGA CATCATCTTT GACCCCAACA TCCTTACCAT TGGTACTGGA 
ATGGAGGAAC ATAACTTGTA TGCTATCAAT TTTATCCATG CAACAAGAGT CATTAAGGAA 
ACCTTACCAG GAGTCAGAAT AAGTGGAGGT CTTTCCAACT TATCCTTCTC CTTCCGAGGG 
ATGGAAGCCA TTCGAGAAGC AATGCATGGT GTGTTCCTTT ATCACGCGAT CAAGTTTGGT 
ATGGACATGG GGATAGTCAA TGCCGGCAAC CTCCCTGTGT ATGATGCTAT CCACAAGGAC 
CTTCTTCAGC TCTGTGAAGA CCTCATCTGG AACAAAGACT CTGAGGCTAC TGAGAAACTC 
TTGCGATATG CCCAGACTCA TGGCACAGGA GGGAAGAAAG TCATCCAGAC AGATGAGTGG 
AGGAATGGCT CCATTGAAGA GCGCCTGGAG TATGCTCTTG TGAAGGGCAT TGAAAAGCAC 
ATTGTTGAAG ATACTGAAGA AGCCAGGTTA AACGGGGAAA AATACCCTCG GCCTCTGAAT 
ATAATTGAAG GGCCCCTAAT GAATGGCATG AAAGTGGTTG GTGATCTTTT TGGAGCTGGA 
AAGATGTTTC TACCTCAGGT TATAAAGTCA GCCCGTGTCA TGAAGAAGGC TGTTGGCCAC 
CTTATCCCTT TCATGGAAAA AGAAAGAGAA GAAGCCAGAT TGATTAATGG TTCGGTTGAA 
GAAGAGGACC CTTACCAAGG CACCATTGTG CTGGCCACTG TTAAAGGGGA CGTGCATGAC 
ATCGGCAAGA ACATAGTTGG CGTGGTGCTG GCCTGTAACA ATTTCAGAGT TATTGATTTA 
GGAGTCATGA CTCCCTGTGA TAAGATACTT CAAGCTGCTC TGGACCACAA AGCAGATATA 
ATTGGCTTGT CAGGACTCAT CACTCCATCC TTGGATGAGA TGATTTTTGT TGCCAAGGAA 
ATGGAGAGGT TGGCTATAAA GATTCCATTG TTGATAGGAG GAGCAACCAC TTCAAGAACC 
CACACAGCAG TTAAAATTGC ACCACGGTAC AGCGCGCCTG TGATCCACGT CCTAGATGCA 
TCCAAGAGTG TGGTGGTGTG TTCTCAGCTG TTAGATGAAA ATCTGAGAGA TGACTACTTT 
GAAGAAATAC TGGAAGAGTA TGAAGATATT AGACAGGACC ACTATGAGTC TCTCAAAGAG 
AGAAAATACG TACCCCTAAG TCAAGCCAGA AAACACGGTT TCCACATTGA TTGGCTCTCT 
GAACCTCATC CAGTGAAGCC CACGTTTATT GGGACCCAGG TCTTTGAGGA CTACAACCTG 
CAAAAGCTGG TGGACTACAT TGACTGGAAG CCTTTCTTTG ACGTCTGGCA GCTCCGGGGC 
AAGTACCCGA ACCGAGGATT TCCCAAGATA TTTAATGACA AAGCAGTAGG TGAAGAAGCC 
AGAAAGGTAT ACAATGATGC TCAGAATATG CTGAACATAC TGATTAGTCA AAAGAAACTG 
CAGGCCAGGG GTGTGGTTGG ATTCTGGCCA GCACAGAGTG TCCAGGATGA CATCCACCTG 
TATGCAGAGG GGGTGGTGCC CCAGGCCGCT GAGCCCATAG CCACCTTCTA TGGACTGAGG 
CAGCAGGCTG AGAAGGACTC TTCTAGTACA GACCCCTACC ACTGCCTCTC AGACTTCATT 
GCTCCTTTGC ATTCTGGTGT CTGTGACTAC TTGGGTCTGT TTGCTGTTGC CTGCTTTGGG 
GTTGAAGAGC TGAGTAAGAC CTATGAGGAT GATGGCGATG ACTACAGCAG CATCATGGTG 
AAGGCGCTGG GCGACAGGCT GGCAGAGGCC TTTGCAGAGG AGCTCCATGA GAGAGTTCGC 
CGAGAACTGT GGGCCTACTC TAGGAGTGAG CAGTTGGGCG TCCCAGACTT GCGCAGACTC 
CGGTATGAGG GCATCCGGCC AGCTCCTGGT TACCCCAGCC AGCCTGACCA TACTGAGAAG 
CTTACCATGT GGAGACTGGC CAGCATCGAG CAGGCCACAG GCATCAGGCT GACAGAATCC 
TTGGCAATGG CACCCGCTTC AGCAGTGTCA GGGCTGTACT TCTCCAACGT AAAGGCCAAA 
TATTTTGCTG TGGGGAAAAT TTCCAAAGAT CAGACTGAGG ATTATGCTTT GAGGAAGAAC 
ATGCCAGTGG CCGAGGTGGA GAAATGGCTT GGTCCCATTC TGGGCTATGA CACAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mtr ( NM_001081128.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074597.1 Mus musculus 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), mRNA.

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CloneID	OMu01747
Clone ID Related Accession (Same CDS sequence)	NM_001081128.3
Accession Version	NM_001081128.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Mus musculus(house mouse)
Product	methionine synthase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB630813.1, BC145683.1 and AI894170.1. On Sep 22, 2011 this sequence version replaced NM_001081128.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145683.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAAGAAAA CCCTGCAGGA TGAGATTGAA GCCATTCTTC GGAAGAGGAT CATGGTGCTA 
GACGGTGGGA TGGGGACCAT GATCCAGCGG TACAAACTAA GTGAAGAACA TTTCCAAGGT 
CAAGAGTTTA AAGATCACAG CAGGCCACTG AAAGGCAACA ATGACATCTT AAGTATAACT 
CAGCCTGATA TTATTTACCA GATTCATAAG GAATACTTGC TGGCTGGAGC AGATATCATC 
GAAACAAACA CTTTCAGCAG TACTAGTATC GCCCAGGCTG ACTATGGCCT TGAACACTTG 
GCCTACCGGA TGAACAAGTG CTCTGCAGAT GTGGCCAGAA AAGCCGCTGA GGAGATAACT 
CTGCAGACAG GAGTCAAGAG GTTTGTGGCT GGAGCTCTGG GTCCGACTAA TAAGACACTT 
TCTGTCTCCC CATCTGTGGA AAGGCCAGAT TATAGGAACA TCACATTTGA TGAGCTTGTT 
GACGCATACC AGGAGCAGGC CAAGGGACTG CTGGATGGCA GGGTCGACAT CTTACTCATT 
GAAACAATTT TTGATACAGC TAATGCCAAA GCAGCCTTGT TTGCGATCCA GAACCTCTTT 
GAAGAGAATT ATGCTCCTCC TCGGCCTATC TTTATTTCTG GGACCATTGT TGATAAAAGT 
GGAAGGACTC TTTCTGGGCA GACGGGAGAG GCTTTTGTCA CCAGCGTGTC TCATTCGGAC 
CCCCTGTGCA TTGGATTAAA TTGTTCTCTG GGTGCAGCTG AAATGAGGCC TTTCATTGAA 
ACAATTGGAA AATGTACAAC AGCCTATGTC CTCTGTTACC CAAATGCAGG TCTTCCCAAC 
ACTTTTGGCG ACTATGATGA AACACCATCC ACGATGGCCA CGCACCTGAA GGACTTTGCT 
GTGGATGGCT TGGTGAATAT AGTTGGTGGA TGCTGTGGTT CAACACCTGA TCACATCAGG 
GAAATTGCGG AAGCTGTGAA AAAGTGTAAG CCAAGAGTCC CCCCTGCTAG TGTTTTTGAA 
GGACATATGT TACTGTCTGG TCTAGAGCCC TTCAGGATTG GACCATACAC CAACTTTGTT 
AACATTGGAG AGCGCTGTAA TGTGGCAGGA TCCAGGAAAT TTGCTAAACT CATCATGGCA 
GGAAACTATG AAGAAGCCCT GAGCATTGCC AAAGCACAGG TGGAAATGGG AGCTCAGGTG 
CTCGATATCA ACATGGACGA CGGCATGCTG GATGGTCCCA GTGCAATGAC CAGATTTTGC 
AACTCCATTG CTTCTGAGCC TGACATTGCC AAGGTGCCCC TGTGCATTGA CTCTTCTAAC 
TTTGCCGTGA TTGAAGCTGG GTTGAAGTGC TGCCAAGGGA AGTGCATAGT CAACAGCATC 
AGCCTCAAGG AGGGGGAGGG GGACTTCCTG GAGAAGGCCC GGAAGATTAA GAAGTTTGGA 
GCTGCTGTGG TGGTTATGGC TTTTGATGAG GAAGGACAAG CAACAGAAAC AGATGTTAAA 
GTCAATGTGT GCACCAGAGC TTACCATCTA CTTGTGGACA AAGTGGGCTT CAATCCAAAT 
GACATCATCT TTGACCCCAA CATCCTTACC ATTGGTACTG GAATGGAGGA ACATAACTTG 
TATGCTATCA ATTTTATCCA TGCAACAAGA GTCATTAAGG AAACCTTACC AGGAGTCAGA 
ATAAGTGGAG GTCTTTCCAA CTTATCCTTC TCCTTCCGAG GGATGGAAGC CATTCGAGAA 
GCAATGCATG GTGTGTTCCT TTATCACGCG ATCAAGTTTG GTATGGACAT GGGGATAGTC 
AATGCCGGCA ACCTCCCTGT GTATGATGCT ATCCACAAGG ACCTTCTTCA GCTCTGTGAA 
GACCTCATCT GGAACAAAGA CTCTGAGGCT ACTGAGAAAC TCTTGCGATA TGCCCAGACT 
CATGGCACAG GAGGGAAGAA AGTCATCCAG ACAGATGAGT GGAGGAATGG CTCCATTGAA 
GAGCGCCTGG AGTATGCTCT TGTGAAGGGC ATTGAAAAGC ACATTGTTGA AGATACTGAA 
GAAGCCAGGT TAAACGGGGA AAAATACCCT CGGCCTCTGA ATATAATTGA AGGGCCCCTA 
ATGAATGGCA TGAAAGTGGT TGGTGATCTT TTTGGAGCTG GAAAGATGTT TCTACCTCAG 
GTTATAAAGT CAGCCCGTGT CATGAAGAAG GCTGTTGGCC ACCTTATCCC TTTCATGGAA 
AAAGAAAGAG AAGAAGCCAG ATTGATTAAT GGTTCGGTTG AAGAAGAGGA CCCTTACCAA 
GGCACCATTG TGCTGGCCAC TGTTAAAGGG GACGTGCATG ACATCGGCAA GAACATAGTT 
GGCGTGGTGC TGGCCTGTAA CAATTTCAGA GTTATTGATT TAGGAGTCAT GACTCCCTGT 
GATAAGATAC TTCAAGCTGC TCTGGACCAC AAAGCAGATA TAATTGGCTT GTCAGGACTC 
ATCACTCCAT CCTTGGATGA GATGATTTTT GTTGCCAAGG AAATGGAGAG GTTGGCTATA 
AAGATTCCAT TGTTGATAGG AGGAGCAACC ACTTCAAGAA CCCACACAGC AGTTAAAATT 
GCACCACGGT ACAGCGCGCC TGTGATCCAC GTCCTAGATG CATCCAAGAG TGTGGTGGTG 
TGTTCTCAGC TGTTAGATGA AAATCTGAGA GATGACTACT TTGAAGAAAT ACTGGAAGAG 
TATGAAGATA TTAGACAGGA CCACTATGAG TCTCTCAAAG AGAGAAAATA CGTACCCCTA 
AGTCAAGCCA GAAAACACGG TTTCCACATT GATTGGCTCT CTGAACCTCA TCCAGTGAAG 
CCCACGTTTA TTGGGACCCA GGTCTTTGAG GACTACAACC TGCAAAAGCT GGTGGACTAC 
ATTGACTGGA AGCCTTTCTT TGACGTCTGG CAGCTCCGGG GCAAGTACCC GAACCGAGGA 
TTTCCCAAGA TATTTAATGA CAAAGCAGTA GGTGAAGAAG CCAGAAAGGT ATACAATGAT 
GCTCAGAATA TGCTGAACAT ACTGATTAGT CAAAAGAAAC TGCAGGCCAG GGGTGTGGTT 
GGATTCTGGC CAGCACAGAG TGTCCAGGAT GACATCCACC TGTATGCAGA GGGGGTGGTG 
CCCCAGGCCG CTGAGCCCAT AGCCACCTTC TATGGACTGA GGCAGCAGGC TGAGAAGGAC 
TCTTCTAGTA CAGACCCCTA CCACTGCCTC TCAGACTTCA TTGCTCCTTT GCATTCTGGT 
GTCTGTGACT ACTTGGGTCT GTTTGCTGTT GCCTGCTTTG GGGTTGAAGA GCTGAGTAAG 
ACCTATGAGG ATGATGGCGA TGACTACAGC AGCATCATGG TGAAGGCGCT GGGCGACAGG 
CTGGCAGAGG CCTTTGCAGA GGAGCTCCAT GAGAGAGTTC GCCGAGAACT GTGGGCCTAC 
TCTAGGAGTG AGCAGTTGGG CGTCCCAGAC TTGCGCAGAC TCCGGTATGA GGGCATCCGG 
CCAGCTCCTG GTTACCCCAG CCAGCCTGAC CATACTGAGA AGCTTACCAT GTGGAGACTG 
GCCAGCATCG AGCAGGCCAC AGGCATCAGG CTGACAGAAT CCTTGGCAAT GGCACCCGCT 
TCAGCAGTGT CAGGGCTGTA CTTCTCCAAC GTAAAGGCCA AATATTTTGC TGTGGGGAAA 
ATTTCCAAAG ATCAGACTGA GGATTATGCT TTGAGGAAGA ACATGCCAGT GGCCGAGGTG 
GAGAAATGGC TTGGTCCCAT TCTGGGCTAT GACACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074597.1
CDS	258..4019
Translation	MKKTLQDEIEAILRKRIMVLDGGMGTMIQRYKLSEEHFQGQEFKDHSRPLKGNNDILSITQPDIIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNKCSADVARKAAEEITLQTGVKRFVAGALGPTNKTLSVSPSVERPDYRNITFDELVDAYQEQAKGLLDGRVDILLIETIFDTANAKAALFAIQNLFEENYAPPRPIFISGTIVDKSGRTLSGQTGEAFVTSVSHSDPLCIGLNCSLGAAEMRPFIETIGKCTTAYVLCYPNAGLPNTFGDYDETPSTMATHLKDFAVDGLVNIVGGCCGSTPDHIREIAEAVKKCKPRVPPASVFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRKFAKLIMAGNYEEALSIAKAQVEMGAQVLDINMDDGMLDGPSAMTRFCNSIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEGDFLEKARKIKKFGAAVVVMAFDEEGQATETDVKVNVCTRAYHLLVDKVGFNPNDIIFDPNILTIGTGMEEHNLYAINFIHATRVIKETLPGVRISGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGNLPVYDAIHKDLLQLCEDLIWNKDSEATEKLLRYAQTHGTGGKKVIQTDEWRNGSIEERLEYALVKGIEKHIVEDTEEARLNGEKYPRPLNIIEGPLMNGMKVVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREEARLINGSVEEEDPYQGTIVLATVKGDVHDIGKNIVGVVLACNNFRVIDLGVMTPCDKILQAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLRDDYFEEILEEYEDIRQDHYESLKERKYVPLSQARKHGFHIDWLSEPHPVKPTFIGTQVFEDYNLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFNDKAVGEEARKVYNDAQNMLNILISQKKLQARGVVGFWPAQSVQDDIHLYAEGVVPQAAEPIATFYGLRQQAEKDSSSTDPYHCLSDFIAPLHSGVCDYLGLFAVACFGVEELSKTYEDDGDDYSSIMVKALGDRLAEAFAEELHERVRRELWAYSRSEQLGVPDLRRLRYEGIRPAPGYPSQPDHTEKLTMWRLASIEQATGIRLTESLAMAPASAVSGLYFSNVKAKYFAVGKISKDQTEDYALRKNMPVAEVEKWLGPILGYDTD

Target ORF information:

RefSeq Version	NM_001081128.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081128.3

ORF Insert Sequence:

ATGAAGAAAA CCCTGCAGGA TGAGATTGAA GCCATTCTTC GGAAGAGGAT CATGGTGCTA 
GACGGTGGGA TGGGGACCAT GATCCAGCGG TACAAACTAA GTGAAGAACA TTTCCAAGGT 
CAAGAGTTTA AAGATCACAG CAGGCCACTG AAAGGCAACA ATGACATCTT AAGTATAACT 
CAGCCTGATA TTATTTACCA GATTCATAAG GAATACTTGC TGGCTGGAGC AGATATCATC 
GAAACAAACA CTTTCAGCAG TACTAGTATC GCCCAGGCTG ACTATGGCCT TGAACACTTG 
GCCTACCGGA TGAACAAGTG CTCTGCAGAT GTGGCCAGAA AAGCCGCTGA GGAGATAACT 
CTGCAGACAG GAGTCAAGAG GTTTGTGGCT GGAGCTCTGG GTCCGACTAA TAAGACACTT 
TCTGTCTCCC CATCTGTGGA AAGGCCAGAT TATAGGAACA TCACATTTGA TGAGCTTGTT 
GACGCATACC AGGAGCAGGC CAAGGGACTG CTGGATGGCA GGGTCGACAT CTTACTCATT 
GAAACAATTT TTGATACAGC TAATGCCAAA GCAGCCTTGT TTGCGATCCA GAACCTCTTT 
GAAGAGAATT ATGCTCCTCC TCGGCCTATC TTTATTTCTG GGACCATTGT TGATAAAAGT 
GGAAGGACTC TTTCTGGGCA GACGGGAGAG GCTTTTGTCA CCAGCGTGTC TCATTCGGAC 
CCCCTGTGCA TTGGATTAAA TTGTTCTCTG GGTGCAGCTG AAATGAGGCC TTTCATTGAA 
ACAATTGGAA AATGTACAAC AGCCTATGTC CTCTGTTACC CAAATGCAGG TCTTCCCAAC 
ACTTTTGGCG ACTATGATGA AACACCATCC ACGATGGCCA CGCACCTGAA GGACTTTGCT 
GTGGATGGCT TGGTGAATAT AGTTGGTGGA TGCTGTGGTT CAACACCTGA TCACATCAGG 
GAAATTGCGG AAGCTGTGAA AAAGTGTAAG CCAAGAGTCC CCCCTGCTAG TGTTTTTGAA 
GGACATATGT TACTGTCTGG TCTAGAGCCC TTCAGGATTG GACCATACAC CAACTTTGTT 
AACATTGGAG AGCGCTGTAA TGTGGCAGGA TCCAGGAAAT TTGCTAAACT CATCATGGCA 
GGAAACTATG AAGAAGCCCT GAGCATTGCC AAAGCACAGG TGGAAATGGG AGCTCAGGTG 
CTCGATATCA ACATGGACGA CGGCATGCTG GATGGTCCCA GTGCAATGAC CAGATTTTGC 
AACTCCATTG CTTCTGAGCC TGACATTGCC AAGGTGCCCC TGTGCATTGA CTCTTCTAAC 
TTTGCCGTGA TTGAAGCTGG GTTGAAGTGC TGCCAAGGGA AGTGCATAGT CAACAGCATC 
AGCCTCAAGG AGGGGGAGGG GGACTTCCTG GAGAAGGCCC GGAAGATTAA GAAGTTTGGA 
GCTGCTGTGG TGGTTATGGC TTTTGATGAG GAAGGACAAG CAACAGAAAC AGATGTTAAA 
GTCAATGTGT GCACCAGAGC TTACCATCTA CTTGTGGACA AAGTGGGCTT CAATCCAAAT 
GACATCATCT TTGACCCCAA CATCCTTACC ATTGGTACTG GAATGGAGGA ACATAACTTG 
TATGCTATCA ATTTTATCCA TGCAACAAGA GTCATTAAGG AAACCTTACC AGGAGTCAGA 
ATAAGTGGAG GTCTTTCCAA CTTATCCTTC TCCTTCCGAG GGATGGAAGC CATTCGAGAA 
GCAATGCATG GTGTGTTCCT TTATCACGCG ATCAAGTTTG GTATGGACAT GGGGATAGTC 
AATGCCGGCA ACCTCCCTGT GTATGATGCT ATCCACAAGG ACCTTCTTCA GCTCTGTGAA 
GACCTCATCT GGAACAAAGA CTCTGAGGCT ACTGAGAAAC TCTTGCGATA TGCCCAGACT 
CATGGCACAG GAGGGAAGAA AGTCATCCAG ACAGATGAGT GGAGGAATGG CTCCATTGAA 
GAGCGCCTGG AGTATGCTCT TGTGAAGGGC ATTGAAAAGC ACATTGTTGA AGATACTGAA 
GAAGCCAGGT TAAACGGGGA AAAATACCCT CGGCCTCTGA ATATAATTGA AGGGCCCCTA 
ATGAATGGCA TGAAAGTGGT TGGTGATCTT TTTGGAGCTG GAAAGATGTT TCTACCTCAG 
GTTATAAAGT CAGCCCGTGT CATGAAGAAG GCTGTTGGCC ACCTTATCCC TTTCATGGAA 
AAAGAAAGAG AAGAAGCCAG ATTGATTAAT GGTTCGGTTG AAGAAGAGGA CCCTTACCAA 
GGCACCATTG TGCTGGCCAC TGTTAAAGGG GACGTGCATG ACATCGGCAA GAACATAGTT 
GGCGTGGTGC TGGCCTGTAA CAATTTCAGA GTTATTGATT TAGGAGTCAT GACTCCCTGT 
GATAAGATAC TTCAAGCTGC TCTGGACCAC AAAGCAGATA TAATTGGCTT GTCAGGACTC 
ATCACTCCAT CCTTGGATGA GATGATTTTT GTTGCCAAGG AAATGGAGAG GTTGGCTATA 
AAGATTCCAT TGTTGATAGG AGGAGCAACC ACTTCAAGAA CCCACACAGC AGTTAAAATT 
GCACCACGGT ACAGCGCGCC TGTGATCCAC GTCCTAGATG CATCCAAGAG TGTGGTGGTG 
TGTTCTCAGC TGTTAGATGA AAATCTGAGA GATGACTACT TTGAAGAAAT ACTGGAAGAG 
TATGAAGATA TTAGACAGGA CCACTATGAG TCTCTCAAAG AGAGAAAATA CGTACCCCTA 
AGTCAAGCCA GAAAACACGG TTTCCACATT GATTGGCTCT CTGAACCTCA TCCAGTGAAG 
CCCACGTTTA TTGGGACCCA GGTCTTTGAG GACTACAACC TGCAAAAGCT GGTGGACTAC 
ATTGACTGGA AGCCTTTCTT TGACGTCTGG CAGCTCCGGG GCAAGTACCC GAACCGAGGA 
TTTCCCAAGA TATTTAATGA CAAAGCAGTA GGTGAAGAAG CCAGAAAGGT ATACAATGAT 
GCTCAGAATA TGCTGAACAT ACTGATTAGT CAAAAGAAAC TGCAGGCCAG GGGTGTGGTT 
GGATTCTGGC CAGCACAGAG TGTCCAGGAT GACATCCACC TGTATGCAGA GGGGGTGGTG 
CCCCAGGCCG CTGAGCCCAT AGCCACCTTC TATGGACTGA GGCAGCAGGC TGAGAAGGAC 
TCTTCTAGTA CAGACCCCTA CCACTGCCTC TCAGACTTCA TTGCTCCTTT GCATTCTGGT 
GTCTGTGACT ACTTGGGTCT GTTTGCTGTT GCCTGCTTTG GGGTTGAAGA GCTGAGTAAG 
ACCTATGAGG ATGATGGCGA TGACTACAGC AGCATCATGG TGAAGGCGCT GGGCGACAGG 
CTGGCAGAGG CCTTTGCAGA GGAGCTCCAT GAGAGAGTTC GCCGAGAACT GTGGGCCTAC 
TCTAGGAGTG AGCAGTTGGG CGTCCCAGAC TTGCGCAGAC TCCGGTATGA GGGCATCCGG 
CCAGCTCCTG GTTACCCCAG CCAGCCTGAC CATACTGAGA AGCTTACCAT GTGGAGACTG 
GCCAGCATCG AGCAGGCCAC AGGCATCAGG CTGACAGAAT CCTTGGCAAT GGCACCCGCT 
TCAGCAGTGT CAGGGCTGTA CTTCTCCAAC GTAAAGGCCA AATATTTTGC TGTGGGGAAA 
ATTTCCAAAG ATCAGACTGA GGATTATGCT TTGAGGAAGA ACATGCCAGT GGCCGAGGTG 
GAGAAATGGC TTGGTCCCAT TCTGGGCTAT GACACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of Hematopoiesis and Methionine Homeostasis by mTORC1 Inhibitor NPRL2.
Cell reports12(3)371-9(2015 Jul)
Dutchak PA,Laxman S,Estill SJ,Wang C,Wang Y,Wang Y,Bulut GB,Gao J,Huang LJ,Tu BP

Spatial and temporal expression of folate-related transporters and metabolic enzymes during mouse placental development.
Placenta33(5)440-8(2012 May)
Cherukad J,Wainwright V,Watson ED

Regulation of homocysteine homeostasis through the transcriptional coactivator PGC-1alpha.
American journal of physiology. Endocrinology and metabolism296(3)E543-8(2009 Mar)
Li S,Arning E,Liu C,Vitvitsky V,Hernandez C,Banerjee R,Bottiglieri T,Lin JD

Glycine N-methyltransferase-/- mice develop chronic hepatitis and glycogen storage disease in the liver.
Hepatology (Baltimore, Md.)46(5)1413-25(2007 Nov)
Liu SP,Li YS,Chen YJ,Chiang EP,Li AF,Lee YH,Tsai TF,Hsiao M,Huang SF,Hwang SF,Chen YM

Genetic and nutritional deficiencies in folate metabolism influence tumorigenicity in Apcmin/+ mice.
The Journal of nutritional biochemistry18(5)305-12(2007 May)
Lawrance AK,Deng L,Brody LC,Finnell RH,Shane B,Rozen R

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Mtr cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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