Npc1l1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npc1l1
Entrez Gene ID	237636
Full Name	NPC1 like intracellular cholesterol transporter 1
Synonyms	9130221N23Rik,Gm243
General protein information	Preferred Names NPC1 like intracellular cholesterol transporter 1 Names Niemann-Pick C1-like protein 1 NPC1-like 1 Niemann-Pick disease, type C1 Niemann-Pick disease, type C1-like 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006514693.3	XP_006514756.1	Niemann-Pick C1-like protein 1 isoform X1
NM_207242.2	NP_997125.2	NPC1-like intracellular cholesterol transporter 1 precursor
XM_006514693.4	XP_006514756.1	NPC1-like intracellular cholesterol transporter 1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NPC1L1	XP_002686936.1
Mus musculus (house mouse)	Npc1l1	NP_997125.2
Homo sapiens (human)	NPC1L1	NP_037521.2
Canis lupus familiaris (dog)	NPC1L1	NP_001091019.1
Danio rerio (zebrafish)	npc1l1	XP_005171497.1
Xenopus tropicalis (tropical clawed frog)	npc1l1	XP_002932620.2
Pan troglodytes (chimpanzee)	NPC1L1	XP_003318442.1
	NPC1L1	NP_001071157.1
Rattus norvegicus (Norway rat)	Npc1l1	NP_001002025.1

Gene Ontology
Bibliography

Related articles in PubMed

Osteopontin deficiency protects mice from cholesterol gallstone formation by reducing expression of intestinal NPC1L1.
Lin J, Shao WQ, Chen QZ, Zhu WW, Lu L, Jia HL, Chen JH
Molecular medicine reports16(2)1785-1792(2017 Aug)

NPC1L1 knockout protects against colitis-associated tumorigenesis in mice.
He J, Shin H, Wei X, Kadegowda AK, Chen R, Xie SK
BMC cancer15189(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Decreased mRNA levels of HNF4alpha and SREBP2 in mice could be involved in the reduction in NPC1L1 expression.
Title: Impact of a high-cholesterol diet on expression levels of Niemann-Pick C1-like 1 and intestinal transporters in rats and mice.

NPC1L1 may suppress gluconeogenesis by inhibition of FoxO1 pathways.
Title: Hepatic NPC1L1 overexpression ameliorates glucose metabolism in diabetic mice via suppression of gluconeogenesis.

NPC1L1 knockout protects against colitis-associated tumorigenesis.
Title: NPC1L1 knockout protects against colitis-associated tumorigenesis in mice.

demonstrate that Niemann-Pick C1-like 1 (NPC1L1) protein, a cholesterol transporter, plays a central role in intestinal VK uptake and modulates the anticoagulant effect of warfarin. In
Title: NPC1L1 is a key regulator of intestinal vitamin K absorption and a modulator of warfarin therapy.

DNA methylation in the promoter region of the NPC1L1 gene appears to be a major mechanism underlying differential expression of NPC1L1 along the length of the gastrointestinal tract.
Title: Epigenetic modulation of intestinal cholesterol transporter Niemann-Pick C1-like 1 (NPC1L1) gene expression by DNA methylation.

The following Npc1l1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npc1l1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu45205	XM_006514693.4 Latest version!	Mus musculus NPC1 like intracellular cholesterol transporter 1 (Npc1l1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu15281	NM_207242.2 Latest version!	Mus musculus NPC1 like intracellular cholesterol transporter 1 (Npc1l1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu45205	XM_006514693.3	Mus musculus NPC1-like 1 (Npc1l1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npc1l1 ( XM_006514693.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514756.1 Mus musculus NPC1-like 1 (Npc1l1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu45205
Clone ID Related Accession (Same CDS sequence)	XM_006514693.3 , XM_006514693.4
Accession Version	XM_006514693.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	Niemann-Pick C1-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514693.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTG CCTGGCAGGG ATGGCTGCTC TGGGCCCTGC TCCTGAATTC GGCCCAGGGT 
GAGCTCTACA CACCCACTCA CAAAGCTGGC TTCTGCACCT TTTATGAAGA GTGTGGGAAG 
AACCCAGAGC TTTCTGGAGG CCTCACATCA CTATCCAATA TCTCCTGCTT GTCTAATACC 
CCAGCCCGCC ATGTCACAGG TGACCACCTG GCTCTTCTCC AGCGCGTCTG TCCCCGCCTA 
TACAATGGCC CCAATGACAC CTATGCCTGT TGCTCTACCA AGCAGCTGGT GTCATTAGAC 
AGTAGCCTGT CTATCACCAA GGCCCTCCTT ACACGCTGCC CGGCATGCTC TGAAAATTTT 
GTGAGCATAC ACTGTCATAA TACCTGCAGC CCTGACCAGA GCCTCTTCAT CAATGTTACT 
CGCGTGGTTC AGCGGGACCC TGGACAGCTT CCTGCTGTGG TGGCCTATGA GGCCTTTTAT 
CAACGCAGTT TTGCAGAGAA GGCCTATGAG TCCTGTAGCC GGGTGCGCAT CCCTGCAGCT 
GCCTCGCTGG CTGTGGGCAG CATGTGTGGA GTGTATGGCT CTGCCCTCTG CAATGCTCAG 
CGCTGGCTCA ACTTCCAAGG AGACACAGGG AATGGCCTGG CTCCGCTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCCTGGCA GATGGGATGA AGCCACTGGA TGGGAAGATC 
ACACCCTGCA ATGAGTCCCA GGGTGAAGAC TCGGCAGCCT GTTCCTGCCA GGACTGTGCA 
GCATCCTGCC CTGTCATCCC TCCGCCCCCG GCCCTGCGCC CTTCTTTCTA CATGGGTCGA 
ATGCCAGGCT GGCTGGCTCT CATCATCATC TTCACTGCTG TCTTTGTATT GCTCTCTGTT 
GTCCTTGTGT ATCTCCGAGT GGCTTCCAAC AGGAACAAGA ACAAGACAGC AGGCTCCCAG 
GAAGCCCCCA ACCTCCCTCG TAAGCGCAGA TTCTCACCTC ACACTGTCCT TGGCCGGTTC 
TTCGAGAGCT GGGGAACAAG GGTGGCCTCA TGGCCACTCA CTGTCTTGGC ACTGTCCTTC 
ATAGTTGTGA TAGCCTTGTC AGTAGGCCTG ACCTTTATAG AACTCACCAC AGACCCTGTG 
GAACTGTGGT CGGCCCCTAA AAGCCAAGCC CGGAAAGAAA AGGCTTTCCA TGACGAGCAT 
TTTGGCCCCT TCTTCCGAAC CAACCAGATT TTTGTGACAG CTAAGAACAG GTCCAGCTAC 
AAGTACGACT CCCTGCTGCT AGGGCCCAAG AACTTCAGTG GGATCCTATC CCTGGACTTG 
CTGCAGGAGC TGTTGGAGCT ACAGGAGAGA CTTCGACACC TGCAAGTGTG GTCCCATGAG 
GCACAGCGCA ACATCTCCCT CCAGGACATC TGCTATGCTC CCCTCAAACC GCATAACACC 
AGCCTCACTG ACTGCTGTGT CAACAGCCTC CTTCAATACT TCCAGAACAA CCACACACTC 
CTGCTGCTCA CAGCCAACCA GACTCTGAAT GGCCAGACCT CCCTGGTGGA CTGGAAGGAC 
CATTTCCTCT ACTGTGCCAA TGCCCCTCTC ACGTACAAAG ATGGCACAGC CCTGGCCCTG 
AGCTGCATAG CTGACTACGG GGCACCTGTC TTCCCCTTCC TTGCTGTTGG GGGCTACCAA 
GGGACGGACT ACTCGGAGGC AGAAGCCCTG ATCATAACCT TCTCTATCAA TAACTACCCC 
GCTGATGATC CCCGCATGGC CCACGCCAAG CTCTGGGAGG AGGCTTTCTT GAAGGAAATG 
CAATCCTTCC AGAGAAGCAC AGCTGACAAG TTCCAGATTG CGTTCTCAGC TGAGCGTTCT 
CTGGAGGACG AGATCAATCG CACTACCATC CAGGACCTGC CTGTCTTTGC CATCAGCTAC 
CTTATCGTCT TCCTGTACAT CTCCCTGGCC CTGGGCAGCT ACTCCAGATG GAGCCGAGTT 
GCGGTGGATT CCAAGGCTAC TCTGGGCCTA GGTGGGGTGG CTGTTGTGCT GGGAGCAGTC 
GTGGCTGCCA TGGGCTTCTA CTCCTACCTG GGTGTCCCCT CCTCTCTGGT CATCATTCAA 
GTGGTACCTT TCCTGGTGCT GGCTGTGGGA GCTGACAACA TCTTCATCTT TGTTCTTGAG 
TACCAGAGGC TGCCTAGGAT GCCCGGGGAG CAGCGAGAGG CTCACATTGG CCGCACCCTG 
GGTAGTGTGG CCCCCAGCAT GCTGCTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTTCTA 
GGGGCCCTGA CCTCCATGCC AGCTGTGAGG ACCTTTGCCT TGACCTCTGG CTTAGCAATC 
ATCTTTGACT TCCTGCTCCA GATGACAGCC TTTGTGGCCC TGCTCTCCCT GGATAGCAAG 
AGGCAGGAGG CCTCTCGCCC CGACGTCGTG TGCTGCTTTT CAAGCCGAAA TCTGCCCCCA 
CCGAAACAAA AAGAAGGCCT CTTACTTTGC TTCTTCCGCA AGATATACAC TCCCTTCCTG 
CTGCACAGAT TCATCCGCCC TGTTGTGCTG CTGCTCTTTC TGGTCCTGTT TGGAGCAAAC 
CTCTACTTAA TGTGCAACAT CAGCGTGGGG CTGGACCAGG ATCTGGCTCT GCCCAAGGAT 
TCCTACCTGA TAGACTACTT CCTCTTTCTG AACCGGTACT TGGAAGTGGG GCCTCCAGTG 
TACTTTGACA CCACCTCAGG CTACAACTTT TCCACCGAGG CAGGCATGAA CGCCATTTGC 
TCTAGTGCAG GCTGTGAGAG CTTCTCCCTA ACCCAGAAAA TCCAGTATGC CAGTGAATTC 
CCTAATCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514756.1
CDS	96..2927
Translation	MAAAWQGWLLWALLLNSAQGELYTPTHKAGFCTFYEECGKNPELSGGLTSLSNISCLSNTPARHVTGDHLALLQRVCPRLYNGPNDTYACCSTKQLVSLDSSLSITKALLTRCPACSENFVSIHCHNTCSPDQSLFINVTRVVQRDPGQLPAVVAYEAFYQRSFAEKAYESCSRVRIPAAASLAVGSMCGVYGSALCNAQRWLNFQGDTGNGLAPLDITFHLLEPGQALADGMKPLDGKITPCNESQGEDSAACSCQDCAASCPVIPPPPALRPSFYMGRMPGWLALIIIFTAVFVLLSVVLVYLRVASNRNKNKTAGSQEAPNLPRKRRFSPHTVLGRFFESWGTRVASWPLTVLALSFIVVIALSVGLTFIELTTDPVELWSAPKSQARKEKAFHDEHFGPFFRTNQIFVTAKNRSSYKYDSLLLGPKNFSGILSLDLLQELLELQERLRHLQVWSHEAQRNISLQDICYAPLKPHNTSLTDCCVNSLLQYFQNNHTLLLLTANQTLNGQTSLVDWKDHFLYCANAPLTYKDGTALALSCIADYGAPVFPFLAVGGYQGTDYSEAEALIITFSINNYPADDPRMAHAKLWEEAFLKEMQSFQRSTADKFQIAFSAERSLEDEINRTTIQDLPVFAISYLIVFLYISLALGSYSRWSRVAVDSKATLGLGGVAVVLGAVVAAMGFYSYLGVPSSLVIIQVVPFLVLAVGADNIFIFVLEYQRLPRMPGEQREAHIGRTLGSVAPSMLLCSLSEAICFFLGALTSMPAVRTFALTSGLAIIFDFLLQMTAFVALLSLDSKRQEASRPDVVCCFSSRNLPPPKQKEGLLLCFFRKIYTPFLLHRFIRPVVLLLFLVLFGANLYLMCNISVGLDQDLALPKDSYLIDYFLFLNRYLEVGPPVYFDTTSGYNFSTEAGMNAICSSAGCESFSLTQKIQYASEFPNH

Target ORF information:

RefSeq Version	XM_006514693.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NPC1-like 1 (Npc1l1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514693.3

ORF Insert Sequence:

ATGGCAGCTG CCTGGCAGGG ATGGCTGCTC TGGGCCCTGC TCCTGAATTC GGCCCAGGGT 
GAGCTCTACA CACCCACTCA CAAAGCTGGC TTCTGCACCT TTTATGAAGA GTGTGGGAAG 
AACCCAGAGC TTTCTGGAGG CCTCACATCA CTATCCAATA TCTCCTGCTT GTCTAATACC 
CCAGCCCGCC ATGTCACAGG TGACCACCTG GCTCTTCTCC AGCGCGTCTG TCCCCGCCTA 
TACAATGGCC CCAATGACAC CTATGCCTGT TGCTCTACCA AGCAGCTGGT GTCATTAGAC 
AGTAGCCTGT CTATCACCAA GGCCCTCCTT ACACGCTGCC CGGCATGCTC TGAAAATTTT 
GTGAGCATAC ACTGTCATAA TACCTGCAGC CCTGACCAGA GCCTCTTCAT CAATGTTACT 
CGCGTGGTTC AGCGGGACCC TGGACAGCTT CCTGCTGTGG TGGCCTATGA GGCCTTTTAT 
CAACGCAGTT TTGCAGAGAA GGCCTATGAG TCCTGTAGCC GGGTGCGCAT CCCTGCAGCT 
GCCTCGCTGG CTGTGGGCAG CATGTGTGGA GTGTATGGCT CTGCCCTCTG CAATGCTCAG 
CGCTGGCTCA ACTTCCAAGG AGACACAGGG AATGGCCTGG CTCCGCTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCCTGGCA GATGGGATGA AGCCACTGGA TGGGAAGATC 
ACACCCTGCA ATGAGTCCCA GGGTGAAGAC TCGGCAGCCT GTTCCTGCCA GGACTGTGCA 
GCATCCTGCC CTGTCATCCC TCCGCCCCCG GCCCTGCGCC CTTCTTTCTA CATGGGTCGA 
ATGCCAGGCT GGCTGGCTCT CATCATCATC TTCACTGCTG TCTTTGTATT GCTCTCTGTT 
GTCCTTGTGT ATCTCCGAGT GGCTTCCAAC AGGAACAAGA ACAAGACAGC AGGCTCCCAG 
GAAGCCCCCA ACCTCCCTCG TAAGCGCAGA TTCTCACCTC ACACTGTCCT TGGCCGGTTC 
TTCGAGAGCT GGGGAACAAG GGTGGCCTCA TGGCCACTCA CTGTCTTGGC ACTGTCCTTC 
ATAGTTGTGA TAGCCTTGTC AGTAGGCCTG ACCTTTATAG AACTCACCAC AGACCCTGTG 
GAACTGTGGT CGGCCCCTAA AAGCCAAGCC CGGAAAGAAA AGGCTTTCCA TGACGAGCAT 
TTTGGCCCCT TCTTCCGAAC CAACCAGATT TTTGTGACAG CTAAGAACAG GTCCAGCTAC 
AAGTACGACT CCCTGCTGCT AGGGCCCAAG AACTTCAGTG GGATCCTATC CCTGGACTTG 
CTGCAGGAGC TGTTGGAGCT ACAGGAGAGA CTTCGACACC TGCAAGTGTG GTCCCATGAG 
GCACAGCGCA ACATCTCCCT CCAGGACATC TGCTATGCTC CCCTCAAACC GCATAACACC 
AGCCTCACTG ACTGCTGTGT CAACAGCCTC CTTCAATACT TCCAGAACAA CCACACACTC 
CTGCTGCTCA CAGCCAACCA GACTCTGAAT GGCCAGACCT CCCTGGTGGA CTGGAAGGAC 
CATTTCCTCT ACTGTGCCAA TGCCCCTCTC ACGTACAAAG ATGGCACAGC CCTGGCCCTG 
AGCTGCATAG CTGACTACGG GGCACCTGTC TTCCCCTTCC TTGCTGTTGG GGGCTACCAA 
GGGACGGACT ACTCGGAGGC AGAAGCCCTG ATCATAACCT TCTCTATCAA TAACTACCCC 
GCTGATGATC CCCGCATGGC CCACGCCAAG CTCTGGGAGG AGGCTTTCTT GAAGGAAATG 
CAATCCTTCC AGAGAAGCAC AGCTGACAAG TTCCAGATTG CGTTCTCAGC TGAGCGTTCT 
CTGGAGGACG AGATCAATCG CACTACCATC CAGGACCTGC CTGTCTTTGC CATCAGCTAC 
CTTATCGTCT TCCTGTACAT CTCCCTGGCC CTGGGCAGCT ACTCCAGATG GAGCCGAGTT 
GCGGTGGATT CCAAGGCTAC TCTGGGCCTA GGTGGGGTGG CTGTTGTGCT GGGAGCAGTC 
GTGGCTGCCA TGGGCTTCTA CTCCTACCTG GGTGTCCCCT CCTCTCTGGT CATCATTCAA 
GTGGTACCTT TCCTGGTGCT GGCTGTGGGA GCTGACAACA TCTTCATCTT TGTTCTTGAG 
TACCAGAGGC TGCCTAGGAT GCCCGGGGAG CAGCGAGAGG CTCACATTGG CCGCACCCTG 
GGTAGTGTGG CCCCCAGCAT GCTGCTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTTCTA 
GGGGCCCTGA CCTCCATGCC AGCTGTGAGG ACCTTTGCCT TGACCTCTGG CTTAGCAATC 
ATCTTTGACT TCCTGCTCCA GATGACAGCC TTTGTGGCCC TGCTCTCCCT GGATAGCAAG 
AGGCAGGAGG CCTCTCGCCC CGACGTCGTG TGCTGCTTTT CAAGCCGAAA TCTGCCCCCA 
CCGAAACAAA AAGAAGGCCT CTTACTTTGC TTCTTCCGCA AGATATACAC TCCCTTCCTG 
CTGCACAGAT TCATCCGCCC TGTTGTGCTG CTGCTCTTTC TGGTCCTGTT TGGAGCAAAC 
CTCTACTTAA TGTGCAACAT CAGCGTGGGG CTGGACCAGG ATCTGGCTCT GCCCAAGGAT 
TCCTACCTGA TAGACTACTT CCTCTTTCTG AACCGGTACT TGGAAGTGGG GCCTCCAGTG 
TACTTTGACA CCACCTCAGG CTACAACTTT TCCACCGAGG CAGGCATGAA CGCCATTTGC 
TCTAGTGCAG GCTGTGAGAG CTTCTCCCTA ACCCAGAAAA TCCAGTATGC CAGTGAATTC 
CCTAATCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npc1l1 ( XM_006514693.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514756.1 Mus musculus NPC1 like intracellular cholesterol transporter 1 (Npc1l1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu45205
Clone ID Related Accession (Same CDS sequence)	XM_006514693.3 , XM_006514693.4
Accession Version	XM_006514693.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NPC1-like intracellular cholesterol transporter 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514693.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTG CCTGGCAGGG ATGGCTGCTC TGGGCCCTGC TCCTGAATTC GGCCCAGGGT 
GAGCTCTACA CACCCACTCA CAAAGCTGGC TTCTGCACCT TTTATGAAGA GTGTGGGAAG 
AACCCAGAGC TTTCTGGAGG CCTCACATCA CTATCCAATA TCTCCTGCTT GTCTAATACC 
CCAGCCCGCC ATGTCACAGG TGACCACCTG GCTCTTCTCC AGCGCGTCTG TCCCCGCCTA 
TACAATGGCC CCAATGACAC CTATGCCTGT TGCTCTACCA AGCAGCTGGT GTCATTAGAC 
AGTAGCCTGT CTATCACCAA GGCCCTCCTT ACACGCTGCC CGGCATGCTC TGAAAATTTT 
GTGAGCATAC ACTGTCATAA TACCTGCAGC CCTGACCAGA GCCTCTTCAT CAATGTTACT 
CGCGTGGTTC AGCGGGACCC TGGACAGCTT CCTGCTGTGG TGGCCTATGA GGCCTTTTAT 
CAACGCAGTT TTGCAGAGAA GGCCTATGAG TCCTGTAGCC GGGTGCGCAT CCCTGCAGCT 
GCCTCGCTGG CTGTGGGCAG CATGTGTGGA GTGTATGGCT CTGCCCTCTG CAATGCTCAG 
CGCTGGCTCA ACTTCCAAGG AGACACAGGG AATGGCCTGG CTCCGCTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCCTGGCA GATGGGATGA AGCCACTGGA TGGGAAGATC 
ACACCCTGCA ATGAGTCCCA GGGTGAAGAC TCGGCAGCCT GTTCCTGCCA GGACTGTGCA 
GCATCCTGCC CTGTCATCCC TCCGCCCCCG GCCCTGCGCC CTTCTTTCTA CATGGGTCGA 
ATGCCAGGCT GGCTGGCTCT CATCATCATC TTCACTGCTG TCTTTGTATT GCTCTCTGTT 
GTCCTTGTGT ATCTCCGAGT GGCTTCCAAC AGGAACAAGA ACAAGACAGC AGGCTCCCAG 
GAAGCCCCCA ACCTCCCTCG TAAGCGCAGA TTCTCACCTC ACACTGTCCT TGGCCGGTTC 
TTCGAGAGCT GGGGAACAAG GGTGGCCTCA TGGCCACTCA CTGTCTTGGC ACTGTCCTTC 
ATAGTTGTGA TAGCCTTGTC AGTAGGCCTG ACCTTTATAG AACTCACCAC AGACCCTGTG 
GAACTGTGGT CGGCCCCTAA AAGCCAAGCC CGGAAAGAAA AGGCTTTCCA TGACGAGCAT 
TTTGGCCCCT TCTTCCGAAC CAACCAGATT TTTGTGACAG CTAAGAACAG GTCCAGCTAC 
AAGTACGACT CCCTGCTGCT AGGGCCCAAG AACTTCAGTG GGATCCTATC CCTGGACTTG 
CTGCAGGAGC TGTTGGAGCT ACAGGAGAGA CTTCGACACC TGCAAGTGTG GTCCCATGAG 
GCACAGCGCA ACATCTCCCT CCAGGACATC TGCTATGCTC CCCTCAAACC GCATAACACC 
AGCCTCACTG ACTGCTGTGT CAACAGCCTC CTTCAATACT TCCAGAACAA CCACACACTC 
CTGCTGCTCA CAGCCAACCA GACTCTGAAT GGCCAGACCT CCCTGGTGGA CTGGAAGGAC 
CATTTCCTCT ACTGTGCCAA TGCCCCTCTC ACGTACAAAG ATGGCACAGC CCTGGCCCTG 
AGCTGCATAG CTGACTACGG GGCACCTGTC TTCCCCTTCC TTGCTGTTGG GGGCTACCAA 
GGGACGGACT ACTCGGAGGC AGAAGCCCTG ATCATAACCT TCTCTATCAA TAACTACCCC 
GCTGATGATC CCCGCATGGC CCACGCCAAG CTCTGGGAGG AGGCTTTCTT GAAGGAAATG 
CAATCCTTCC AGAGAAGCAC AGCTGACAAG TTCCAGATTG CGTTCTCAGC TGAGCGTTCT 
CTGGAGGACG AGATCAATCG CACTACCATC CAGGACCTGC CTGTCTTTGC CATCAGCTAC 
CTTATCGTCT TCCTGTACAT CTCCCTGGCC CTGGGCAGCT ACTCCAGATG GAGCCGAGTT 
GCGGTGGATT CCAAGGCTAC TCTGGGCCTA GGTGGGGTGG CTGTTGTGCT GGGAGCAGTC 
GTGGCTGCCA TGGGCTTCTA CTCCTACCTG GGTGTCCCCT CCTCTCTGGT CATCATTCAA 
GTGGTACCTT TCCTGGTGCT GGCTGTGGGA GCTGACAACA TCTTCATCTT TGTTCTTGAG 
TACCAGAGGC TGCCTAGGAT GCCCGGGGAG CAGCGAGAGG CTCACATTGG CCGCACCCTG 
GGTAGTGTGG CCCCCAGCAT GCTGCTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTTCTA 
GGGGCCCTGA CCTCCATGCC AGCTGTGAGG ACCTTTGCCT TGACCTCTGG CTTAGCAATC 
ATCTTTGACT TCCTGCTCCA GATGACAGCC TTTGTGGCCC TGCTCTCCCT GGATAGCAAG 
AGGCAGGAGG CCTCTCGCCC CGACGTCGTG TGCTGCTTTT CAAGCCGAAA TCTGCCCCCA 
CCGAAACAAA AAGAAGGCCT CTTACTTTGC TTCTTCCGCA AGATATACAC TCCCTTCCTG 
CTGCACAGAT TCATCCGCCC TGTTGTGCTG CTGCTCTTTC TGGTCCTGTT TGGAGCAAAC 
CTCTACTTAA TGTGCAACAT CAGCGTGGGG CTGGACCAGG ATCTGGCTCT GCCCAAGGAT 
TCCTACCTGA TAGACTACTT CCTCTTTCTG AACCGGTACT TGGAAGTGGG GCCTCCAGTG 
TACTTTGACA CCACCTCAGG CTACAACTTT TCCACCGAGG CAGGCATGAA CGCCATTTGC 
TCTAGTGCAG GCTGTGAGAG CTTCTCCCTA ACCCAGAAAA TCCAGTATGC CAGTGAATTC 
CCTAATCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514756.1
CDS	114..2945
Translation	MAAAWQGWLLWALLLNSAQGELYTPTHKAGFCTFYEECGKNPELSGGLTSLSNISCLSNTPARHVTGDHLALLQRVCPRLYNGPNDTYACCSTKQLVSLDSSLSITKALLTRCPACSENFVSIHCHNTCSPDQSLFINVTRVVQRDPGQLPAVVAYEAFYQRSFAEKAYESCSRVRIPAAASLAVGSMCGVYGSALCNAQRWLNFQGDTGNGLAPLDITFHLLEPGQALADGMKPLDGKITPCNESQGEDSAACSCQDCAASCPVIPPPPALRPSFYMGRMPGWLALIIIFTAVFVLLSVVLVYLRVASNRNKNKTAGSQEAPNLPRKRRFSPHTVLGRFFESWGTRVASWPLTVLALSFIVVIALSVGLTFIELTTDPVELWSAPKSQARKEKAFHDEHFGPFFRTNQIFVTAKNRSSYKYDSLLLGPKNFSGILSLDLLQELLELQERLRHLQVWSHEAQRNISLQDICYAPLKPHNTSLTDCCVNSLLQYFQNNHTLLLLTANQTLNGQTSLVDWKDHFLYCANAPLTYKDGTALALSCIADYGAPVFPFLAVGGYQGTDYSEAEALIITFSINNYPADDPRMAHAKLWEEAFLKEMQSFQRSTADKFQIAFSAERSLEDEINRTTIQDLPVFAISYLIVFLYISLALGSYSRWSRVAVDSKATLGLGGVAVVLGAVVAAMGFYSYLGVPSSLVIIQVVPFLVLAVGADNIFIFVLEYQRLPRMPGEQREAHIGRTLGSVAPSMLLCSLSEAICFFLGALTSMPAVRTFALTSGLAIIFDFLLQMTAFVALLSLDSKRQEASRPDVVCCFSSRNLPPPKQKEGLLLCFFRKIYTPFLLHRFIRPVVLLLFLVLFGANLYLMCNISVGLDQDLALPKDSYLIDYFLFLNRYLEVGPPVYFDTTSGYNFSTEAGMNAICSSAGCESFSLTQKIQYASEFPNH

Target ORF information:

RefSeq Version	XM_006514693.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NPC1 like intracellular cholesterol transporter 1 (Npc1l1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514693.4

ORF Insert Sequence:

ATGGCAGCTG CCTGGCAGGG ATGGCTGCTC TGGGCCCTGC TCCTGAATTC GGCCCAGGGT 
GAGCTCTACA CACCCACTCA CAAAGCTGGC TTCTGCACCT TTTATGAAGA GTGTGGGAAG 
AACCCAGAGC TTTCTGGAGG CCTCACATCA CTATCCAATA TCTCCTGCTT GTCTAATACC 
CCAGCCCGCC ATGTCACAGG TGACCACCTG GCTCTTCTCC AGCGCGTCTG TCCCCGCCTA 
TACAATGGCC CCAATGACAC CTATGCCTGT TGCTCTACCA AGCAGCTGGT GTCATTAGAC 
AGTAGCCTGT CTATCACCAA GGCCCTCCTT ACACGCTGCC CGGCATGCTC TGAAAATTTT 
GTGAGCATAC ACTGTCATAA TACCTGCAGC CCTGACCAGA GCCTCTTCAT CAATGTTACT 
CGCGTGGTTC AGCGGGACCC TGGACAGCTT CCTGCTGTGG TGGCCTATGA GGCCTTTTAT 
CAACGCAGTT TTGCAGAGAA GGCCTATGAG TCCTGTAGCC GGGTGCGCAT CCCTGCAGCT 
GCCTCGCTGG CTGTGGGCAG CATGTGTGGA GTGTATGGCT CTGCCCTCTG CAATGCTCAG 
CGCTGGCTCA ACTTCCAAGG AGACACAGGG AATGGCCTGG CTCCGCTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCCTGGCA GATGGGATGA AGCCACTGGA TGGGAAGATC 
ACACCCTGCA ATGAGTCCCA GGGTGAAGAC TCGGCAGCCT GTTCCTGCCA GGACTGTGCA 
GCATCCTGCC CTGTCATCCC TCCGCCCCCG GCCCTGCGCC CTTCTTTCTA CATGGGTCGA 
ATGCCAGGCT GGCTGGCTCT CATCATCATC TTCACTGCTG TCTTTGTATT GCTCTCTGTT 
GTCCTTGTGT ATCTCCGAGT GGCTTCCAAC AGGAACAAGA ACAAGACAGC AGGCTCCCAG 
GAAGCCCCCA ACCTCCCTCG TAAGCGCAGA TTCTCACCTC ACACTGTCCT TGGCCGGTTC 
TTCGAGAGCT GGGGAACAAG GGTGGCCTCA TGGCCACTCA CTGTCTTGGC ACTGTCCTTC 
ATAGTTGTGA TAGCCTTGTC AGTAGGCCTG ACCTTTATAG AACTCACCAC AGACCCTGTG 
GAACTGTGGT CGGCCCCTAA AAGCCAAGCC CGGAAAGAAA AGGCTTTCCA TGACGAGCAT 
TTTGGCCCCT TCTTCCGAAC CAACCAGATT TTTGTGACAG CTAAGAACAG GTCCAGCTAC 
AAGTACGACT CCCTGCTGCT AGGGCCCAAG AACTTCAGTG GGATCCTATC CCTGGACTTG 
CTGCAGGAGC TGTTGGAGCT ACAGGAGAGA CTTCGACACC TGCAAGTGTG GTCCCATGAG 
GCACAGCGCA ACATCTCCCT CCAGGACATC TGCTATGCTC CCCTCAAACC GCATAACACC 
AGCCTCACTG ACTGCTGTGT CAACAGCCTC CTTCAATACT TCCAGAACAA CCACACACTC 
CTGCTGCTCA CAGCCAACCA GACTCTGAAT GGCCAGACCT CCCTGGTGGA CTGGAAGGAC 
CATTTCCTCT ACTGTGCCAA TGCCCCTCTC ACGTACAAAG ATGGCACAGC CCTGGCCCTG 
AGCTGCATAG CTGACTACGG GGCACCTGTC TTCCCCTTCC TTGCTGTTGG GGGCTACCAA 
GGGACGGACT ACTCGGAGGC AGAAGCCCTG ATCATAACCT TCTCTATCAA TAACTACCCC 
GCTGATGATC CCCGCATGGC CCACGCCAAG CTCTGGGAGG AGGCTTTCTT GAAGGAAATG 
CAATCCTTCC AGAGAAGCAC AGCTGACAAG TTCCAGATTG CGTTCTCAGC TGAGCGTTCT 
CTGGAGGACG AGATCAATCG CACTACCATC CAGGACCTGC CTGTCTTTGC CATCAGCTAC 
CTTATCGTCT TCCTGTACAT CTCCCTGGCC CTGGGCAGCT ACTCCAGATG GAGCCGAGTT 
GCGGTGGATT CCAAGGCTAC TCTGGGCCTA GGTGGGGTGG CTGTTGTGCT GGGAGCAGTC 
GTGGCTGCCA TGGGCTTCTA CTCCTACCTG GGTGTCCCCT CCTCTCTGGT CATCATTCAA 
GTGGTACCTT TCCTGGTGCT GGCTGTGGGA GCTGACAACA TCTTCATCTT TGTTCTTGAG 
TACCAGAGGC TGCCTAGGAT GCCCGGGGAG CAGCGAGAGG CTCACATTGG CCGCACCCTG 
GGTAGTGTGG CCCCCAGCAT GCTGCTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTTCTA 
GGGGCCCTGA CCTCCATGCC AGCTGTGAGG ACCTTTGCCT TGACCTCTGG CTTAGCAATC 
ATCTTTGACT TCCTGCTCCA GATGACAGCC TTTGTGGCCC TGCTCTCCCT GGATAGCAAG 
AGGCAGGAGG CCTCTCGCCC CGACGTCGTG TGCTGCTTTT CAAGCCGAAA TCTGCCCCCA 
CCGAAACAAA AAGAAGGCCT CTTACTTTGC TTCTTCCGCA AGATATACAC TCCCTTCCTG 
CTGCACAGAT TCATCCGCCC TGTTGTGCTG CTGCTCTTTC TGGTCCTGTT TGGAGCAAAC 
CTCTACTTAA TGTGCAACAT CAGCGTGGGG CTGGACCAGG ATCTGGCTCT GCCCAAGGAT 
TCCTACCTGA TAGACTACTT CCTCTTTCTG AACCGGTACT TGGAAGTGGG GCCTCCAGTG 
TACTTTGACA CCACCTCAGG CTACAACTTT TCCACCGAGG CAGGCATGAA CGCCATTTGC 
TCTAGTGCAG GCTGTGAGAG CTTCTCCCTA ACCCAGAAAA TCCAGTATGC CAGTGAATTC 
CCTAATCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npc1l1 ( NM_207242.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_997125.2 Mus musculus NPC1 like intracellular cholesterol transporter 1 (Npc1l1), mRNA.

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CloneID	OMu15281
Clone ID Related Accession (Same CDS sequence)	NM_207242.2
Accession Version	NM_207242.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	NPC1-like intracellular cholesterol transporter 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB872216.1, BC131788.1, AV377091.2 and CJ191424.1. On Jul 15, 2009 this sequence version replaced NM_207242.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK078947.1, AY437866.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGCTG CCTGGCAGGG ATGGCTGCTC TGGGCCCTGC TCCTGAATTC GGCCCAGGGT 
GAGCTCTACA CACCCACTCA CAAAGCTGGC TTCTGCACCT TTTATGAAGA GTGTGGGAAG 
AACCCAGAGC TTTCTGGAGG CCTCACATCA CTATCCAATA TCTCCTGCTT GTCTAATACC 
CCAGCCCGCC ATGTCACAGG TGACCACCTG GCTCTTCTCC AGCGCGTCTG TCCCCGCCTA 
TACAATGGCC CCAATGACAC CTATGCCTGT TGCTCTACCA AGCAGCTGGT GTCATTAGAC 
AGTAGCCTGT CTATCACCAA GGCCCTCCTT ACACGCTGCC CGGCATGCTC TGAAAATTTT 
GTGAGCATAC ACTGTCATAA TACCTGCAGC CCTGACCAGA GCCTCTTCAT CAATGTTACT 
CGCGTGGTTC AGCGGGACCC TGGACAGCTT CCTGCTGTGG TGGCCTATGA GGCCTTTTAT 
CAACGCAGTT TTGCAGAGAA GGCCTATGAG TCCTGTAGCC GGGTGCGCAT CCCTGCAGCT 
GCCTCGCTGG CTGTGGGCAG CATGTGTGGA GTGTATGGCT CTGCCCTCTG CAATGCTCAG 
CGCTGGCTCA ACTTCCAAGG AGACACAGGG AATGGCCTGG CTCCGCTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCCTGGCA GATGGGATGA AGCCACTGGA TGGGAAGATC 
ACACCCTGCA ATGAGTCCCA GGGTGAAGAC TCGGCAGCCT GTTCCTGCCA GGACTGTGCA 
GCATCCTGCC CTGTCATCCC TCCGCCCCCG GCCCTGCGCC CTTCTTTCTA CATGGGTCGA 
ATGCCAGGCT GGCTGGCTCT CATCATCATC TTCACTGCTG TCTTTGTATT GCTCTCTGTT 
GTCCTTGTGT ATCTCCGAGT GGCTTCCAAC AGGAACAAGA ACAAGACAGC AGGCTCCCAG 
GAAGCCCCCA ACCTCCCTCG TAAGCGCAGA TTCTCACCTC ACACTGTCCT TGGCCGGTTC 
TTCGAGAGCT GGGGAACAAG GGTGGCCTCA TGGCCACTCA CTGTCTTGGC ACTGTCCTTC 
ATAGTTGTGA TAGCCTTGTC AGTAGGCCTG ACCTTTATAG AACTCACCAC AGACCCTGTG 
GAACTGTGGT CGGCCCCTAA AAGCCAAGCC CGGAAAGAAA AGGCTTTCCA TGACGAGCAT 
TTTGGCCCCT TCTTCCGAAC CAACCAGATT TTTGTGACAG CTAAGAACAG GTCCAGCTAC 
AAGTACGACT CCCTGCTGCT AGGGCCCAAG AACTTCAGTG GGATCCTATC CCTGGACTTG 
CTGCAGGAGC TGTTGGAGCT ACAGGAGAGA CTTCGACACC TGCAAGTGTG GTCCCATGAG 
GCACAGCGCA ACATCTCCCT CCAGGACATC TGCTATGCTC CCCTCAAACC GCATAACACC 
AGCCTCACTG ACTGCTGTGT CAACAGCCTC CTTCAATACT TCCAGAACAA CCACACACTC 
CTGCTGCTCA CAGCCAACCA GACTCTGAAT GGCCAGACCT CCCTGGTGGA CTGGAAGGAC 
CATTTCCTCT ACTGTGCCAA TGCCCCTCTC ACGTACAAAG ATGGCACAGC CCTGGCCCTG 
AGCTGCATAG CTGACTACGG GGCACCTGTC TTCCCCTTCC TTGCTGTTGG GGGCTACCAA 
GGGACGGACT ACTCGGAGGC AGAAGCCCTG ATCATAACCT TCTCTATCAA TAACTACCCC 
GCTGATGATC CCCGCATGGC CCACGCCAAG CTCTGGGAGG AGGCTTTCTT GAAGGAAATG 
CAATCCTTCC AGAGAAGCAC AGCTGACAAG TTCCAGATTG CGTTCTCAGC TGAGCGTTCT 
CTGGAGGACG AGATCAATCG CACTACCATC CAGGACCTGC CTGTCTTTGC CATCAGCTAC 
CTTATCGTCT TCCTGTACAT CTCCCTGGCC CTGGGCAGCT ACTCCAGATG GAGCCGAGTT 
GCGGTGGATT CCAAGGCTAC TCTGGGCCTA GGTGGGGTGG CTGTTGTGCT GGGAGCAGTC 
GTGGCTGCCA TGGGCTTCTA CTCCTACCTG GGTGTCCCCT CCTCTCTGGT CATCATTCAA 
GTGGTACCTT TCCTGGTGCT GGCTGTGGGA GCTGACAACA TCTTCATCTT TGTTCTTGAG 
TACCAGAGGC TGCCTAGGAT GCCCGGGGAG CAGCGAGAGG CTCACATTGG CCGCACCCTG 
GGTAGTGTGG CCCCCAGCAT GCTGCTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTTCTA 
GGGGCCCTGA CCTCCATGCC AGCTGTGAGG ACCTTTGCCT TGACCTCTGG CTTAGCAATC 
ATCTTTGACT TCCTGCTCCA GATGACAGCC TTTGTGGCCC TGCTCTCCCT GGATAGCAAG 
AGGCAGGAGG CCTCTCGCCC CGACGTCGTG TGCTGCTTTT CAAGCCGAAA TCTGCCCCCA 
CCGAAACAAA AAGAAGGCCT CTTACTTTGC TTCTTCCGCA AGATATACAC TCCCTTCCTG 
CTGCACAGAT TCATCCGCCC TGTTGTGCTG CTGCTCTTTC TGGTCCTGTT TGGAGCAAAC 
CTCTACTTAA TGTGCAACAT CAGCGTGGGG CTGGACCAGG ATCTGGCTCT GCCCAAGGAT 
TCCTACCTGA TAGACTACTT CCTCTTTCTG AACCGGTACT TGGAAGTGGG GCCTCCAGTG 
TACTTTGACA CCACCTCAGG CTACAACTTT TCCACCGAGG CAGGCATGAA CGCCATTTGC 
TCTAGTGCAG GCTGTGAGAG CTTCTCCCTA ACCCAGAAAA TCCAGTATGC CAGTGAATTC 
CCTAATCAGT CTTATGTGGC TATTGCTGCA TCCTCCTGGG TAGATGACTT CATCGACTGG 
CTGACCCCAT CCTCCTCCTG CTGCCGCATT TATACCCGTG GCCCCCATAA AGATGAGTTC 
TGTCCCTCAA CGGATACTTC CTTCAACTGT CTCAAAAACT GCATGAACCG CACTCTGGGT 
CCCGTGAGAC CCACAACAGA ACAGTTTCAT AAGTACCTGC CCTGGTTCCT GAATGATACG 
CCCAACATCA GATGTCCTAA AGGGGGCCTA GCAGCGTATA GAACCTCTGT GAATTTGAGC 
TCAGATGGCC AGATTATAGC CTCCCAGTTC ATGGCCTACC ACAAGCCCTT ACGGAACTCA 
CAGGACTTTA CAGAAGCTCT CCGGGCATCC CGGTTGCTAG CAGCCAACAT CACAGCTGAA 
CTACGGAAGG TGCCTGGGAC AGATCCCAAC TTTGAGGTCT TCCCTTACAC GATCTCCAAT 
GTGTTCTACC AGCAATACCT GACGGTTCTC CCTGAGGGAA TCTTCACTCT TGCTCTCTGC 
TTCGTGCCCA CCTTTGTGGT CTGCTACCTC CTACTGGGCC TGGACATACG CTCAGGCATC 
CTCAACCTGC TCTCCATCAT TATGATCCTC GTGGACACCA TCGGCCTCAT GGCTGTGTGG 
GGTATCAGCT ACAATGCTGT GTCCCTCATC AACCTTGTCA CGGCAGTGGG CATGTCTGTG 
GAGTTCGTGT CCCACATTAC CCGGTCCTTT GCTGTAAGCA CCAAGCCTAC CCGGCTGGAG 
AGAGCCAAAG ATGCTACTAT CTTCATGGGC AGTGCGGTGT TTGCTGGAGT GGCCATGACC 
AACTTCCCGG GCATCCTCAT CCTGGGCTTT GCTCAGGCCC AGCTTATCCA GATTTTCTTC 
TTCCGCCTCA ACCTCCTGAT CACCTTGCTG GGTCTGCTAC ACGGCCTGGT CTTCCTGCCC 
GTTGTCCTCA GCTATCTGGG GCCAGATGTT AACCAAGCTC TGGTACTGGA GGAGAAACTA 
GCCACTGAGG CAGCCATGGT CTCAGAGCCT TCTTGCCCAC AGTACCCCTT CCCGGCTGAT 
GCAAACACCA GTGACTATGT TAACTACGGC TTTAATCCAG AATTTATCCC TGAAATTAAT 
GCTGCTAGCA GCTCTCTGCC CAAAAGTGAC CAAAAGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997125.2
CDS	123..4124
Translation	MAAAWQGWLLWALLLNSAQGELYTPTHKAGFCTFYEECGKNPELSGGLTSLSNISCLSNTPARHVTGDHLALLQRVCPRLYNGPNDTYACCSTKQLVSLDSSLSITKALLTRCPACSENFVSIHCHNTCSPDQSLFINVTRVVQRDPGQLPAVVAYEAFYQRSFAEKAYESCSRVRIPAAASLAVGSMCGVYGSALCNAQRWLNFQGDTGNGLAPLDITFHLLEPGQALADGMKPLDGKITPCNESQGEDSAACSCQDCAASCPVIPPPPALRPSFYMGRMPGWLALIIIFTAVFVLLSVVLVYLRVASNRNKNKTAGSQEAPNLPRKRRFSPHTVLGRFFESWGTRVASWPLTVLALSFIVVIALSVGLTFIELTTDPVELWSAPKSQARKEKAFHDEHFGPFFRTNQIFVTAKNRSSYKYDSLLLGPKNFSGILSLDLLQELLELQERLRHLQVWSHEAQRNISLQDICYAPLKPHNTSLTDCCVNSLLQYFQNNHTLLLLTANQTLNGQTSLVDWKDHFLYCANAPLTYKDGTALALSCIADYGAPVFPFLAVGGYQGTDYSEAEALIITFSINNYPADDPRMAHAKLWEEAFLKEMQSFQRSTADKFQIAFSAERSLEDEINRTTIQDLPVFAISYLIVFLYISLALGSYSRWSRVAVDSKATLGLGGVAVVLGAVVAAMGFYSYLGVPSSLVIIQVVPFLVLAVGADNIFIFVLEYQRLPRMPGEQREAHIGRTLGSVAPSMLLCSLSEAICFFLGALTSMPAVRTFALTSGLAIIFDFLLQMTAFVALLSLDSKRQEASRPDVVCCFSSRNLPPPKQKEGLLLCFFRKIYTPFLLHRFIRPVVLLLFLVLFGANLYLMCNISVGLDQDLALPKDSYLIDYFLFLNRYLEVGPPVYFDTTSGYNFSTEAGMNAICSSAGCESFSLTQKIQYASEFPNQSYVAIAASSWVDDFIDWLTPSSSCCRIYTRGPHKDEFCPSTDTSFNCLKNCMNRTLGPVRPTTEQFHKYLPWFLNDTPNIRCPKGGLAAYRTSVNLSSDGQIIASQFMAYHKPLRNSQDFTEALRASRLLAANITAELRKVPGTDPNFEVFPYTISNVFYQQYLTVLPEGIFTLALCFVPTFVVCYLLLGLDIRSGILNLLSIIMILVDTIGLMAVWGISYNAVSLINLVTAVGMSVEFVSHITRSFAVSTKPTRLERAKDATIFMGSAVFAGVAMTNFPGILILGFAQAQLIQIFFFRLNLLITLLGLLHGLVFLPVVLSYLGPDVNQALVLEEKLATEAAMVSEPSCPQYPFPADANTSDYVNYGFNPEFIPEINAASSSLPKSDQKF

Target ORF information:

RefSeq Version	NM_207242.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NPC1 like intracellular cholesterol transporter 1 (Npc1l1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207242.2

ORF Insert Sequence:

ATGGCAGCTG CCTGGCAGGG ATGGCTGCTC TGGGCCCTGC TCCTGAATTC GGCCCAGGGT 
GAGCTCTACA CACCCACTCA CAAAGCTGGC TTCTGCACCT TTTATGAAGA GTGTGGGAAG 
AACCCAGAGC TTTCTGGAGG CCTCACATCA CTATCCAATA TCTCCTGCTT GTCTAATACC 
CCAGCCCGCC ATGTCACAGG TGACCACCTG GCTCTTCTCC AGCGCGTCTG TCCCCGCCTA 
TACAATGGCC CCAATGACAC CTATGCCTGT TGCTCTACCA AGCAGCTGGT GTCATTAGAC 
AGTAGCCTGT CTATCACCAA GGCCCTCCTT ACACGCTGCC CGGCATGCTC TGAAAATTTT 
GTGAGCATAC ACTGTCATAA TACCTGCAGC CCTGACCAGA GCCTCTTCAT CAATGTTACT 
CGCGTGGTTC AGCGGGACCC TGGACAGCTT CCTGCTGTGG TGGCCTATGA GGCCTTTTAT 
CAACGCAGTT TTGCAGAGAA GGCCTATGAG TCCTGTAGCC GGGTGCGCAT CCCTGCAGCT 
GCCTCGCTGG CTGTGGGCAG CATGTGTGGA GTGTATGGCT CTGCCCTCTG CAATGCTCAG 
CGCTGGCTCA ACTTCCAAGG AGACACAGGG AATGGCCTGG CTCCGCTGGA CATCACCTTC 
CACCTCTTGG AGCCTGGCCA GGCCCTGGCA GATGGGATGA AGCCACTGGA TGGGAAGATC 
ACACCCTGCA ATGAGTCCCA GGGTGAAGAC TCGGCAGCCT GTTCCTGCCA GGACTGTGCA 
GCATCCTGCC CTGTCATCCC TCCGCCCCCG GCCCTGCGCC CTTCTTTCTA CATGGGTCGA 
ATGCCAGGCT GGCTGGCTCT CATCATCATC TTCACTGCTG TCTTTGTATT GCTCTCTGTT 
GTCCTTGTGT ATCTCCGAGT GGCTTCCAAC AGGAACAAGA ACAAGACAGC AGGCTCCCAG 
GAAGCCCCCA ACCTCCCTCG TAAGCGCAGA TTCTCACCTC ACACTGTCCT TGGCCGGTTC 
TTCGAGAGCT GGGGAACAAG GGTGGCCTCA TGGCCACTCA CTGTCTTGGC ACTGTCCTTC 
ATAGTTGTGA TAGCCTTGTC AGTAGGCCTG ACCTTTATAG AACTCACCAC AGACCCTGTG 
GAACTGTGGT CGGCCCCTAA AAGCCAAGCC CGGAAAGAAA AGGCTTTCCA TGACGAGCAT 
TTTGGCCCCT TCTTCCGAAC CAACCAGATT TTTGTGACAG CTAAGAACAG GTCCAGCTAC 
AAGTACGACT CCCTGCTGCT AGGGCCCAAG AACTTCAGTG GGATCCTATC CCTGGACTTG 
CTGCAGGAGC TGTTGGAGCT ACAGGAGAGA CTTCGACACC TGCAAGTGTG GTCCCATGAG 
GCACAGCGCA ACATCTCCCT CCAGGACATC TGCTATGCTC CCCTCAAACC GCATAACACC 
AGCCTCACTG ACTGCTGTGT CAACAGCCTC CTTCAATACT TCCAGAACAA CCACACACTC 
CTGCTGCTCA CAGCCAACCA GACTCTGAAT GGCCAGACCT CCCTGGTGGA CTGGAAGGAC 
CATTTCCTCT ACTGTGCCAA TGCCCCTCTC ACGTACAAAG ATGGCACAGC CCTGGCCCTG 
AGCTGCATAG CTGACTACGG GGCACCTGTC TTCCCCTTCC TTGCTGTTGG GGGCTACCAA 
GGGACGGACT ACTCGGAGGC AGAAGCCCTG ATCATAACCT TCTCTATCAA TAACTACCCC 
GCTGATGATC CCCGCATGGC CCACGCCAAG CTCTGGGAGG AGGCTTTCTT GAAGGAAATG 
CAATCCTTCC AGAGAAGCAC AGCTGACAAG TTCCAGATTG CGTTCTCAGC TGAGCGTTCT 
CTGGAGGACG AGATCAATCG CACTACCATC CAGGACCTGC CTGTCTTTGC CATCAGCTAC 
CTTATCGTCT TCCTGTACAT CTCCCTGGCC CTGGGCAGCT ACTCCAGATG GAGCCGAGTT 
GCGGTGGATT CCAAGGCTAC TCTGGGCCTA GGTGGGGTGG CTGTTGTGCT GGGAGCAGTC 
GTGGCTGCCA TGGGCTTCTA CTCCTACCTG GGTGTCCCCT CCTCTCTGGT CATCATTCAA 
GTGGTACCTT TCCTGGTGCT GGCTGTGGGA GCTGACAACA TCTTCATCTT TGTTCTTGAG 
TACCAGAGGC TGCCTAGGAT GCCCGGGGAG CAGCGAGAGG CTCACATTGG CCGCACCCTG 
GGTAGTGTGG CCCCCAGCAT GCTGCTGTGC AGCCTCTCTG AGGCCATCTG CTTCTTTCTA 
GGGGCCCTGA CCTCCATGCC AGCTGTGAGG ACCTTTGCCT TGACCTCTGG CTTAGCAATC 
ATCTTTGACT TCCTGCTCCA GATGACAGCC TTTGTGGCCC TGCTCTCCCT GGATAGCAAG 
AGGCAGGAGG CCTCTCGCCC CGACGTCGTG TGCTGCTTTT CAAGCCGAAA TCTGCCCCCA 
CCGAAACAAA AAGAAGGCCT CTTACTTTGC TTCTTCCGCA AGATATACAC TCCCTTCCTG 
CTGCACAGAT TCATCCGCCC TGTTGTGCTG CTGCTCTTTC TGGTCCTGTT TGGAGCAAAC 
CTCTACTTAA TGTGCAACAT CAGCGTGGGG CTGGACCAGG ATCTGGCTCT GCCCAAGGAT 
TCCTACCTGA TAGACTACTT CCTCTTTCTG AACCGGTACT TGGAAGTGGG GCCTCCAGTG 
TACTTTGACA CCACCTCAGG CTACAACTTT TCCACCGAGG CAGGCATGAA CGCCATTTGC 
TCTAGTGCAG GCTGTGAGAG CTTCTCCCTA ACCCAGAAAA TCCAGTATGC CAGTGAATTC 
CCTAATCAGT CTTATGTGGC TATTGCTGCA TCCTCCTGGG TAGATGACTT CATCGACTGG 
CTGACCCCAT CCTCCTCCTG CTGCCGCATT TATACCCGTG GCCCCCATAA AGATGAGTTC 
TGTCCCTCAA CGGATACTTC CTTCAACTGT CTCAAAAACT GCATGAACCG CACTCTGGGT 
CCCGTGAGAC CCACAACAGA ACAGTTTCAT AAGTACCTGC CCTGGTTCCT GAATGATACG 
CCCAACATCA GATGTCCTAA AGGGGGCCTA GCAGCGTATA GAACCTCTGT GAATTTGAGC 
TCAGATGGCC AGATTATAGC CTCCCAGTTC ATGGCCTACC ACAAGCCCTT ACGGAACTCA 
CAGGACTTTA CAGAAGCTCT CCGGGCATCC CGGTTGCTAG CAGCCAACAT CACAGCTGAA 
CTACGGAAGG TGCCTGGGAC AGATCCCAAC TTTGAGGTCT TCCCTTACAC GATCTCCAAT 
GTGTTCTACC AGCAATACCT GACGGTTCTC CCTGAGGGAA TCTTCACTCT TGCTCTCTGC 
TTCGTGCCCA CCTTTGTGGT CTGCTACCTC CTACTGGGCC TGGACATACG CTCAGGCATC 
CTCAACCTGC TCTCCATCAT TATGATCCTC GTGGACACCA TCGGCCTCAT GGCTGTGTGG 
GGTATCAGCT ACAATGCTGT GTCCCTCATC AACCTTGTCA CGGCAGTGGG CATGTCTGTG 
GAGTTCGTGT CCCACATTAC CCGGTCCTTT GCTGTAAGCA CCAAGCCTAC CCGGCTGGAG 
AGAGCCAAAG ATGCTACTAT CTTCATGGGC AGTGCGGTGT TTGCTGGAGT GGCCATGACC 
AACTTCCCGG GCATCCTCAT CCTGGGCTTT GCTCAGGCCC AGCTTATCCA GATTTTCTTC 
TTCCGCCTCA ACCTCCTGAT CACCTTGCTG GGTCTGCTAC ACGGCCTGGT CTTCCTGCCC 
GTTGTCCTCA GCTATCTGGG GCCAGATGTT AACCAAGCTC TGGTACTGGA GGAGAAACTA 
GCCACTGAGG CAGCCATGGT CTCAGAGCCT TCTTGCCCAC AGTACCCCTT CCCGGCTGAT 
GCAAACACCA GTGACTATGT TAACTACGGC TTTAATCCAG AATTTATCCC TGAAATTAAT 
GCTGCTAGCA GCTCTCTGCC CAAAAGTGAC CAAAAGTTCT AA