Topbp1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Topbp1
Entrez Gene ID	235559
Full Name	topoisomerase (DNA) II binding protein 1
Synonyms	1110031N14Rik,2810429C13Rik,AI256758,D430026L04Rik,mKIAA0259
General protein information	Preferred Names topoisomerase (DNA) II binding protein 1 Names DNA topoisomerase 2-binding protein 1 DNA topoisomerase II-beta-binding protein 1 DNA topoisomerase II-binding protein 1 topoisomerase (DNA) II beta binding protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 F1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006511706.4	XP_006511769.2	DNA topoisomerase 2-binding protein 1 isoform X1
XM_006511707.3	XP_006511770.1	DNA topoisomerase 2-binding protein 1 isoform X2
XM_006511706.3	XP_006511769.2	DNA topoisomerase 2-binding protein 1 isoform X1
XM_006511707.4	XP_006511770.1	DNA topoisomerase 2-binding protein 1 isoform X2
NM_176979.5	NP_795953.2	DNA topoisomerase 2-binding protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TOPBP1	NP_001193695.1
Rattus norvegicus (Norway rat)	Topbp1	XP_236578.6
Homo sapiens (human)	TOPBP1	NP_008958.2
Danio rerio (zebrafish)	topbp1	NP_001186667.1
Xenopus tropicalis (tropical clawed frog)	topbp1	XP_004915426.1
	TOPBP1	XP_001112636.2
Mus musculus (house mouse)	Topbp1	NP_795953.2
Gallus gallus (chicken)	TOPBP1	XP_418794.1
Pan troglodytes (chimpanzee)	TOPBP1	XP_516761.3
Canis lupus familiaris (dog)	TOPBP1	XP_005634503.1

Gene Ontology
Bibliography

Related articles in PubMed

DNA damage response protein TOPBP1 regulates X chromosome silencing in the mammalian germ line.
ElInati E, Russell HR, Ojarikre OA, Sangrithi M, Hirota T, de Rooij DG, McKinnon PJ, Turner JMA
Proceedings of the National Academy of Sciences of the United States of America114(47)12536-12541(2017 Nov)

Elucidation of the Fanconi Anemia Protein Network in Meiosis and Its Function in the Regulation of Histone Modifications.
Alavattam KG, Kato Y, Sin HS, Maezawa S, Kowalski IJ, Zhang F, Pang Q, Andreassen PR, Namekawa SH
Cell reports17(4)1141-1157(2016 10)

Levels of the E2 interacting protein TopBP1 modulate papillomavirus maintenance stage replication.
Kanginakudru S, DeSmet M, Thomas Y, Morgan IM, Androphy EJ
Virology478129-35(2015 Apr)

SCML2 establishes the male germline epigenome through regulation of histone H2A ubiquitination.
Hasegawa K, Sin HS, Maezawa S, Broering TJ, Kartashov AV, Alavattam KG, Ichijima Y, Zhang F, Bacon WC, Greis KD, Andreassen PR, Barski A, Namekawa SH
Developmental cell32(5)574-88(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Consistent with prior reports, TopBP1 co-localized in discrete nuclear foci and was in complex with papillomavirus E2 protein.
Title: Levels of the E2 interacting protein TopBP1 modulate papillomavirus maintenance stage replication.

Studies identify the SIRT1-TopBP1 axis as a key signaling mode in the regulation of the metabolic checkpoint and the DNA damage checkpoint.
Title: A divergent role of the SIRT1-TopBP1 axis in regulating metabolic checkpoint and DNA damage checkpoint.

TopBP1 is a crucial factor in V(D)J rearrangement during the development of B, T and iNKT cells.
Title: TopBP1 deficiency impairs V(D)J recombination during lymphocyte development.

The deacetylated form of TopBP1 in SIRT1 mutant cells repressed replication origin firing, while the acetylated form of TopBP1 lost this function.
Title: SIRT1 deacetylates TopBP1 and modulates intra-S-phase checkpoint and DNA replication origin firing.

Our data suggest that, unlike the yeast models, the TopBP1-AAD is the major activator of ATR, sustaining cell proliferation and embryonic development
Title: An essential function for the ATR-activation-domain (AAD) of TopBP1 in mouse development and cellular senescence.

The following Topbp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Topbp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu73844	XM_006511706.4 Latest version!	Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu19740	XM_006511707.4 Latest version!	Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu19740	NM_176979.5 Latest version!	Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu73844	XM_006511706.3	Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu19740	XM_006511707.3	Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Topbp1 ( XM_006511706.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511769.2 Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73844
Clone ID Related Accession (Same CDS sequence)	XM_006511706.4 , XM_006511706.3
Accession Version	XM_006511706.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA topoisomerase 2-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511706.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG 
ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT 
CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA 
GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA 
CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT 
AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA 
CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT 
ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT 
GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT 
GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT 
GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC 
TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT 
AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG 
GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA 
GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC 
TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG 
ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG 
CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT 
GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC 
CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC 
AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT 
TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG 
CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA 
CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT 
ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC 
TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG 
GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA 
TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG 
AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC 
ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG 
GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT 
TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG 
GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG 
CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT 
TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT 
GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA 
CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT 
TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG 
GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG 
AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC 
GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT 
AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGCCAGAT 
CATCTGTCTG TAGAAGGAAA TGAGACAAAC ACTATGGGCA CAAATGACAA AGAGTCACCT 
CTGTTGAATG GGAGTGGAAG AGATGACTGT AAAGGAGCTC TGACCCAGGC CTTGGAGATG 
AGAGAGAACT TCCAGAAGCA GCTGCAAGAG ATCATGTCAG CCACCTGCAT TGTGAAGACG 
CCTGCCCAGA AGACTTGCAT GTCAAGAAGC AGCTGTAACA GCGCCTCTTC AACTCCTGAC 
AGCGCTCGCT CTGTGCGGAG CGGACGAAGC CGAGTCCTGG AGGCACTCAG GCAGTCTCGG 
CAGGCAGTGC CTGATGTGAA CACGGAGCCG TCCCAGAACG AGCAGATCAT TTGGGACGAC 
CCGACAGCAA GGGAAGAGAG AGCAAGGCTT GCCAGCAACT TACAGTGGCC CAGCGACCCC 
ACACAGCATT CTGAGCTTCA GGTTGAGATA AAAATGCCGG ATGACTCTCC CTCTCGAAAG 
CCTGTGTACC ATTCAGAAAT TGCTGAGCAG GCCTCGTGTG TTACTCAAGC CCCAGGGCAC 
CCAGGCTCTG AAGAACCAGA GCCTCCAGTA GCAGAAAGGC CTTTGATCCC CGAGCCGCAA 
GCCCCCGCTG TAGCTTCCCC CCTGGCTAAG CCGCCTGTGG CCCCACAACC TGCAGACAAG 
ATTGAAACAC AGGAGGAGAC GCACAGAAAA GTTAAAAAAC AGTATGTGTT CCAGATGTCG 
TCTCTGAATT CTCAAGAGCG GATTGATTAC TGTCGCCTGA TTAAAGACTT AGGTGGGTCG 
GTGATCGAGA AGCAGTGCTC TGACCCCAGC TGCACACACA TGGTTGTGGG CTACCCACTG 
CGCAATGAGA AGTACTTAGC TTCTATGGCA GCTGGTAAAT GGGTGCTTCA TCGCTCCTAC 
CTAGACGCTT GCAAGACAGC TGGGCGCTTT GTGCAGGAAG AAGACTACGA GTGGGGAAGT 
AGCTCCATAC TTGATGCTCT CCCTGACGTC ACTGAGCACC AGCAGAAGCT CGCACTGGCG 
GCAATGAGAT GGAGGAAAAG AATCCAGCAA AGTCAAGAAT CCGGCATTGT TGAGGGAGCA 
TTTAGTGGCT GGAAAGCTAT TTTACGTGTT GATCGGCCCC GAGAAGCTGG GTTCAAACGC 
CTTCTTCAGG CAGGAGGAGC AAAGGTGTTA TCTGGTCATC CAGAACCTTT ACTTAAAGAT 
GCTACGCATC TCTTTTGTGA CTTCAATAAA CTGAAACCTG ATGACTGCAG AGTTTTCATC 
GCAGAGGCTA CTGCCCAGAA CATGGTCTGC TTGAAAACAG AATATATCGC TGATTATCTC 
ATGCTGGAAT CCCCTCCTTG TGCAGACAAT TACCGTGTAT CCGAAGCTGC TTTATTCCAT 
AATAAGAAAG GTGGGCCTGG ATTACCACAG AAGAGGAAAA CTCCTGCAGA GAATGTAGTC 
AAACGGCCTC GAGTACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511769.2
CDS	213..4613
Translation	MSRNDQEPFLVKFLKSSDNSECFFKALESIKELQSEDYLQIITDEEALKIRENDKSLYICDRFSGTVFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDVTVSCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYKAETRVEAKMVPDTSTPTAQSNAESHTLADVSHISNINGSCVNETMFGSTTSKLECSLENLENLDISMFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIVGDYDDDVRQFWSKSSHRPHVVGAKWLLECFTKGYILPEESYIHTNYQPAGIAVSDQPGNQTAVLDKSGSFSKSALVPAERLQQADEDLLAQYGNDDSTMVEAKLSEALEPEVGPCPGSAHREPCDDSTHISVQEENKSSVSHCILDDSTVREEGLFSQKSFLVLGFSVENKCNIVDIIREHAGKIVSLPSRIVADYAVVPLLGCEVDVTVGEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGVTPLEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKANAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLETARIGKRADENHFLVDNAPKQEQVLETKIPNGVSSNPDLPAHPDAHLEIHRKKAVTPLDMNRFQSRAFRAVISQQRGQDPTFPPVRQPLTKEPSLHLDTPSKFLSKDKLFKPSFDVTDALAALETPNAASQKRKLSSPLSEVIVRNLTVALANSSRNTDSHSASPQLKGAHLEEEETRKPLDSVVVCVSKKLSKKQSELNGVAASLGAEYRWSFDETVTHFIYQGRANDSNREYKSAKERGVHIVSEHWLLEPDHLSVEGNETNTMGTNDKESPLLNGSGRDDCKGALTQALEMRENFQKQLQEIMSATCIVKTPAQKTCMSRSSCNSASSTPDSARSVRSGRSRVLEALRQSRQAVPDVNTEPSQNEQIIWDDPTAREERARLASNLQWPSDPTQHSELQVEIKMPDDSPSRKPVYHSEIAEQASCVTQAPGHPGSEEPEPPVAERPLIPEPQAPAVASPLAKPPVAPQPADKIETQEETHRKVKKQYVFQMSSLNSQERIDYCRLIKDLGGSVIEKQCSDPSCTHMVVGYPLRNEKYLASMAAGKWVLHRSYLDACKTAGRFVQEEDYEWGSSSILDALPDVTEHQQKLALAAMRWRKRIQQSQESGIVEGAFSGWKAILRVDRPREAGFKRLLQAGGAKVLSGHPEPLLKDATHLFCDFNKLKPDDCRVFIAEATAQNMVCLKTEYIADYLMLESPPCADNYRVSEAALFHNKKGGPGLPQKRKTPAENVVKRPRVH

Target ORF information:

RefSeq Version	XM_006511706.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511706.4

ORF Insert Sequence:

ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG 
ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT 
CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA 
GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA 
CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT 
AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA 
CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT 
ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT 
GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT 
GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT 
GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC 
TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT 
AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG 
GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA 
GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC 
TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG 
ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG 
CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT 
GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC 
CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC 
AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT 
TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG 
CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA 
CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT 
ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC 
TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG 
GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA 
TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG 
AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC 
ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG 
GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT 
TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG 
GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG 
CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT 
TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT 
GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA 
CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT 
TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG 
GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG 
AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC 
GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT 
AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGCCAGAT 
CATCTGTCTG TAGAAGGAAA TGAGACAAAC ACTATGGGCA CAAATGACAA AGAGTCACCT 
CTGTTGAATG GGAGTGGAAG AGATGACTGT AAAGGAGCTC TGACCCAGGC CTTGGAGATG 
AGAGAGAACT TCCAGAAGCA GCTGCAAGAG ATCATGTCAG CCACCTGCAT TGTGAAGACG 
CCTGCCCAGA AGACTTGCAT GTCAAGAAGC AGCTGTAACA GCGCCTCTTC AACTCCTGAC 
AGCGCTCGCT CTGTGCGGAG CGGACGAAGC CGAGTCCTGG AGGCACTCAG GCAGTCTCGG 
CAGGCAGTGC CTGATGTGAA CACGGAGCCG TCCCAGAACG AGCAGATCAT TTGGGACGAC 
CCGACAGCAA GGGAAGAGAG AGCAAGGCTT GCCAGCAACT TACAGTGGCC CAGCGACCCC 
ACACAGCATT CTGAGCTTCA GGTTGAGATA AAAATGCCGG ATGACTCTCC CTCTCGAAAG 
CCTGTGTACC ATTCAGAAAT TGCTGAGCAG GCCTCGTGTG TTACTCAAGC CCCAGGGCAC 
CCAGGCTCTG AAGAACCAGA GCCTCCAGTA GCAGAAAGGC CTTTGATCCC CGAGCCGCAA 
GCCCCCGCTG TAGCTTCCCC CCTGGCTAAG CCGCCTGTGG CCCCACAACC TGCAGACAAG 
ATTGAAACAC AGGAGGAGAC GCACAGAAAA GTTAAAAAAC AGTATGTGTT CCAGATGTCG 
TCTCTGAATT CTCAAGAGCG GATTGATTAC TGTCGCCTGA TTAAAGACTT AGGTGGGTCG 
GTGATCGAGA AGCAGTGCTC TGACCCCAGC TGCACACACA TGGTTGTGGG CTACCCACTG 
CGCAATGAGA AGTACTTAGC TTCTATGGCA GCTGGTAAAT GGGTGCTTCA TCGCTCCTAC 
CTAGACGCTT GCAAGACAGC TGGGCGCTTT GTGCAGGAAG AAGACTACGA GTGGGGAAGT 
AGCTCCATAC TTGATGCTCT CCCTGACGTC ACTGAGCACC AGCAGAAGCT CGCACTGGCG 
GCAATGAGAT GGAGGAAAAG AATCCAGCAA AGTCAAGAAT CCGGCATTGT TGAGGGAGCA 
TTTAGTGGCT GGAAAGCTAT TTTACGTGTT GATCGGCCCC GAGAAGCTGG GTTCAAACGC 
CTTCTTCAGG CAGGAGGAGC AAAGGTGTTA TCTGGTCATC CAGAACCTTT ACTTAAAGAT 
GCTACGCATC TCTTTTGTGA CTTCAATAAA CTGAAACCTG ATGACTGCAG AGTTTTCATC 
GCAGAGGCTA CTGCCCAGAA CATGGTCTGC TTGAAAACAG AATATATCGC TGATTATCTC 
ATGCTGGAAT CCCCTCCTTG TGCAGACAAT TACCGTGTAT CCGAAGCTGC TTTATTCCAT 
AATAAGAAAG GTGGGCCTGG ATTACCACAG AAGAGGAAAA CTCCTGCAGA GAATGTAGTC 
AAACGGCCTC GAGTACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Topbp1 ( XM_006511707.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511770.1 Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu19740
Clone ID Related Accession (Same CDS sequence)	XM_006511707.3 , XM_006511707.4 , NM_176979.5
Accession Version	XM_006511707.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA topoisomerase 2-binding protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511707.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG 
ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT 
CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA 
GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA 
CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT 
AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA 
CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT 
ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT 
GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT 
GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT 
GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC 
TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT 
AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG 
GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA 
GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC 
TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG 
ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG 
CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT 
GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC 
CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC 
AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT 
TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG 
CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA 
CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT 
ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC 
TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG 
GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA 
TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG 
AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC 
ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG 
GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT 
TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG 
GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG 
CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT 
TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT 
GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA 
CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT 
TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG 
GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG 
AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC 
GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT 
AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGTGTGCT 
CAAGAGTATA AGCATCTTCC TGAATCTCTT TATCCACACA CTTACAATCC CAAAATGAGT 
TTGGACATCA ACACAGTGCA AGATGGGCGG CTCTGTAACA GCCGGGCACC CTTGGCAGTT 
TCTGCATCTA AGGATGATGG GCCAGATCAT CTGTCTGTAG AAGGAAATGA GACAAACACT 
ATGGGCACAA ATGACAAAGA GTCACCTCTG TTGAATGGGA GTGGAAGAGA TGACTGTAAA 
GGAGCTCTGA CCCAGGCCTT GGAGATGAGA GAGAACTTCC AGAAGCAGCT GCAAGAGATC 
ATGTCAGCCA CCTGCATTGT GAAGACGCCT GCCCAGAAGA CTTGCATGTC AAGAAGCAGC 
TGTAACAGCG CCTCTTCAAC TCCTGACAGC GCTCGCTCTG TGCGGAGCGG ACGAAGCCGA 
GTCCTGGAGG CACTCAGGCA GTCTCGGCAG GCAGTGCCTG ATGTGAACAC GGAGCCGTCC 
CAGAACGAGC AGATCATTTG GGACGACCCG ACAGCAAGGG AAGAGAGAGC AAGGCTTGCC 
AGCAACTTAC AGTGGCCCAG CGACCCCACA CAGCATTCTG AGCTTCAGGT TGAGATAAAA 
ATGCCGGATG ACTCTCCCTC TCGAAAGCCT GTGTACCATT CAGAAATTGC TGAGCAGGCC 
TCGTGTGTTA CTCAAGCCCC AGGGCACCCA GGCTCTGAAG AACCAGAGCC TCCAGTAGCA 
GAAAGGCCTT TGATCCCCGA GCCGCAAGCC CCCGCTGTAG CTTCCCCCCT GGCTAAGCCG 
CCTGTGGCCC CACAACCTGC AGACAAGATT GAAACACAGG AGGAGACGCA CAGAAAAGTT 
AAAAAACAGT ATGTGTTCCA GATGTCGTCT CTGAATTCTC AAGAGCGGAT TGATTACTGT 
CGCCTGATTA AAGACTTAGG TGGGTCGGTG ATCGAGAAGC AGTGCTCTGA CCCCAGCTGC 
ACACACATGG TTGTGGGCTA CCCACTGCGC AATGAGAAGT ACTTAGCTTC TATGGCAGCT 
GGTAAATGGG TGCTTCATCG CTCCTACCTA GACGCTTGCA AGACAGCTGG GCGCTTTGTG 
CAGGAAGAAG ACTACGAGTG GGGAAGTAGC TCCATACTTG ATGCTCTCCC TGACGTCACT 
GAGCACCAGC AGAAGCTCGC ACTGGCGGCA ATGAGATGGA GGAAAAGAAT CCAGCAAAGT 
CAAGAATCCG GCATTGTTGA GGGAGCATTT AGTGGCTGGA AAGCTATTTT ACGTGTTGAT 
CGGCCCCGAG AAGCTGGGTT CAAACGCCTT CTTCAGGCAG GAGGAGCAAA GGTGTTATCT 
GGTCATCCAG AACCTTTACT TAAAGATGCT ACGCATCTCT TTTGTGACTT CAATAAACTG 
AAACCTGATG ACTGCAGAGT TTTCATCGCA GAGGCTACTG CCCAGAACAT GGTCTGCTTG 
AAAACAGAAT ATATCGCTGA TTATCTCATG CTGGAATCCC CTCCTTGTGC AGACAATTAC 
CGTGTATCCG AAGCTGCTTT ATTCCATAAT AAGAAAGGTG GGCCTGGATT ACCACAGAAG 
AGGAAAACTC CTGCAGAGAA TGTAGTCAAA CGGCCTCGAG TACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511770.1
CDS	243..4790
Translation	MSRNDQEPFLVKFLKSSDNSECFFKALESIKELQSEDYLQIITDEEALKIRENDKSLYICDRFSGTVFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDVTVSCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYKAETRVEAKMVPDTSTPTAQSNAESHTLADVSHISNINGSCVNETMFGSTTSKLECSLENLENLDISMFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIVGDYDDDVRQFWSKSSHRPHVVGAKWLLECFTKGYILPEESYIHTNYQPAGIAVSDQPGNQTAVLDKSGSFSKSALVPAERLQQADEDLLAQYGNDDSTMVEAKLSEALEPEVGPCPGSAHREPCDDSTHISVQEENKSSVSHCILDDSTVREEGLFSQKSFLVLGFSVENKCNIVDIIREHAGKIVSLPSRIVADYAVVPLLGCEVDVTVGEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGVTPLEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKANAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLETARIGKRADENHFLVDNAPKQEQVLETKIPNGVSSNPDLPAHPDAHLEIHRKKAVTPLDMNRFQSRAFRAVISQQRGQDPTFPPVRQPLTKEPSLHLDTPSKFLSKDKLFKPSFDVTDALAALETPNAASQKRKLSSPLSEVIVRNLTVALANSSRNTDSHSASPQLKGAHLEEEETRKPLDSVVVCVSKKLSKKQSELNGVAASLGAEYRWSFDETVTHFIYQGRANDSNREYKSAKERGVHIVSEHWLLECAQEYKHLPESLYPHTYNPKMSLDINTVQDGRLCNSRAPLAVSASKDDGPDHLSVEGNETNTMGTNDKESPLLNGSGRDDCKGALTQALEMRENFQKQLQEIMSATCIVKTPAQKTCMSRSSCNSASSTPDSARSVRSGRSRVLEALRQSRQAVPDVNTEPSQNEQIIWDDPTAREERARLASNLQWPSDPTQHSELQVEIKMPDDSPSRKPVYHSEIAEQASCVTQAPGHPGSEEPEPPVAERPLIPEPQAPAVASPLAKPPVAPQPADKIETQEETHRKVKKQYVFQMSSLNSQERIDYCRLIKDLGGSVIEKQCSDPSCTHMVVGYPLRNEKYLASMAAGKWVLHRSYLDACKTAGRFVQEEDYEWGSSSILDALPDVTEHQQKLALAAMRWRKRIQQSQESGIVEGAFSGWKAILRVDRPREAGFKRLLQAGGAKVLSGHPEPLLKDATHLFCDFNKLKPDDCRVFIAEATAQNMVCLKTEYIADYLMLESPPCADNYRVSEAALFHNKKGGPGLPQKRKTPAENVVKRPRVH

Target ORF information:

RefSeq Version	XM_006511707.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511707.4

ORF Insert Sequence:

ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG 
ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT 
CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA 
GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA 
CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT 
AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA 
CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT 
ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT 
GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT 
GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT 
GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC 
TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT 
AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG 
GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA 
GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC 
TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG 
ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG 
CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT 
GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC 
CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC 
AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT 
TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG 
CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA 
CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT 
ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC 
TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG 
GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA 
TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG 
AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC 
ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG 
GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT 
TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG 
GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG 
CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT 
TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT 
GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA 
CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT 
TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG 
GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG 
AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC 
GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT 
AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGTGTGCT 
CAAGAGTATA AGCATCTTCC TGAATCTCTT TATCCACACA CTTACAATCC CAAAATGAGT 
TTGGACATCA ACACAGTGCA AGATGGGCGG CTCTGTAACA GCCGGGCACC CTTGGCAGTT 
TCTGCATCTA AGGATGATGG GCCAGATCAT CTGTCTGTAG AAGGAAATGA GACAAACACT 
ATGGGCACAA ATGACAAAGA GTCACCTCTG TTGAATGGGA GTGGAAGAGA TGACTGTAAA 
GGAGCTCTGA CCCAGGCCTT GGAGATGAGA GAGAACTTCC AGAAGCAGCT GCAAGAGATC 
ATGTCAGCCA CCTGCATTGT GAAGACGCCT GCCCAGAAGA CTTGCATGTC AAGAAGCAGC 
TGTAACAGCG CCTCTTCAAC TCCTGACAGC GCTCGCTCTG TGCGGAGCGG ACGAAGCCGA 
GTCCTGGAGG CACTCAGGCA GTCTCGGCAG GCAGTGCCTG ATGTGAACAC GGAGCCGTCC 
CAGAACGAGC AGATCATTTG GGACGACCCG ACAGCAAGGG AAGAGAGAGC AAGGCTTGCC 
AGCAACTTAC AGTGGCCCAG CGACCCCACA CAGCATTCTG AGCTTCAGGT TGAGATAAAA 
ATGCCGGATG ACTCTCCCTC TCGAAAGCCT GTGTACCATT CAGAAATTGC TGAGCAGGCC 
TCGTGTGTTA CTCAAGCCCC AGGGCACCCA GGCTCTGAAG AACCAGAGCC TCCAGTAGCA 
GAAAGGCCTT TGATCCCCGA GCCGCAAGCC CCCGCTGTAG CTTCCCCCCT GGCTAAGCCG 
CCTGTGGCCC CACAACCTGC AGACAAGATT GAAACACAGG AGGAGACGCA CAGAAAAGTT 
AAAAAACAGT ATGTGTTCCA GATGTCGTCT CTGAATTCTC AAGAGCGGAT TGATTACTGT 
CGCCTGATTA AAGACTTAGG TGGGTCGGTG ATCGAGAAGC AGTGCTCTGA CCCCAGCTGC 
ACACACATGG TTGTGGGCTA CCCACTGCGC AATGAGAAGT ACTTAGCTTC TATGGCAGCT 
GGTAAATGGG TGCTTCATCG CTCCTACCTA GACGCTTGCA AGACAGCTGG GCGCTTTGTG 
CAGGAAGAAG ACTACGAGTG GGGAAGTAGC TCCATACTTG ATGCTCTCCC TGACGTCACT 
GAGCACCAGC AGAAGCTCGC ACTGGCGGCA ATGAGATGGA GGAAAAGAAT CCAGCAAAGT 
CAAGAATCCG GCATTGTTGA GGGAGCATTT AGTGGCTGGA AAGCTATTTT ACGTGTTGAT 
CGGCCCCGAG AAGCTGGGTT CAAACGCCTT CTTCAGGCAG GAGGAGCAAA GGTGTTATCT 
GGTCATCCAG AACCTTTACT TAAAGATGCT ACGCATCTCT TTTGTGACTT CAATAAACTG 
AAACCTGATG ACTGCAGAGT TTTCATCGCA GAGGCTACTG CCCAGAACAT GGTCTGCTTG 
AAAACAGAAT ATATCGCTGA TTATCTCATG CTGGAATCCC CTCCTTGTGC AGACAATTAC 
CGTGTATCCG AAGCTGCTTT ATTCCATAAT AAGAAAGGTG GGCCTGGATT ACCACAGAAG 
AGGAAAACTC CTGCAGAGAA TGTAGTCAAA CGGCCTCGAG TACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Topbp1 ( NM_176979.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_795953.2 Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), mRNA.

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CloneID	OMu19740
Clone ID Related Accession (Same CDS sequence)	XM_006511707.3 , XM_006511707.4 , NM_176979.5
Accession Version	NM_176979.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Mus musculus(house mouse)
Product	DNA topoisomerase 2-binding protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC122747.11. On Nov 17, 2006 this sequence version replaced NM_176979.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC049797.1, AK129104.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG 
ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT 
CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA 
GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA 
CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT 
AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA 
CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT 
ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT 
GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT 
GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT 
GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC 
TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT 
AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG 
GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA 
GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC 
TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG 
ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG 
CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT 
GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC 
CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC 
AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT 
TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG 
CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA 
CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT 
ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC 
TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG 
GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA 
TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG 
AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC 
ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG 
GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT 
TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG 
GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG 
CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT 
TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT 
GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA 
CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT 
TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG 
GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG 
AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC 
GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT 
AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGTGTGCT 
CAAGAGTATA AGCATCTTCC TGAATCTCTT TATCCACACA CTTACAATCC CAAAATGAGT 
TTGGACATCA ACACAGTGCA AGATGGGCGG CTCTGTAACA GCCGGGCACC CTTGGCAGTT 
TCTGCATCTA AGGATGATGG GCCAGATCAT CTGTCTGTAG AAGGAAATGA GACAAACACT 
ATGGGCACAA ATGACAAAGA GTCACCTCTG TTGAATGGGA GTGGAAGAGA TGACTGTAAA 
GGAGCTCTGA CCCAGGCCTT GGAGATGAGA GAGAACTTCC AGAAGCAGCT GCAAGAGATC 
ATGTCAGCCA CCTGCATTGT GAAGACGCCT GCCCAGAAGA CTTGCATGTC AAGAAGCAGC 
TGTAACAGCG CCTCTTCAAC TCCTGACAGC GCTCGCTCTG TGCGGAGCGG ACGAAGCCGA 
GTCCTGGAGG CACTCAGGCA GTCTCGGCAG GCAGTGCCTG ATGTGAACAC GGAGCCGTCC 
CAGAACGAGC AGATCATTTG GGACGACCCG ACAGCAAGGG AAGAGAGAGC AAGGCTTGCC 
AGCAACTTAC AGTGGCCCAG CGACCCCACA CAGCATTCTG AGCTTCAGGT TGAGATAAAA 
ATGCCGGATG ACTCTCCCTC TCGAAAGCCT GTGTACCATT CAGAAATTGC TGAGCAGGCC 
TCGTGTGTTA CTCAAGCCCC AGGGCACCCA GGCTCTGAAG AACCAGAGCC TCCAGTAGCA 
GAAAGGCCTT TGATCCCCGA GCCGCAAGCC CCCGCTGTAG CTTCCCCCCT GGCTAAGCCG 
CCTGTGGCCC CACAACCTGC AGACAAGATT GAAACACAGG AGGAGACGCA CAGAAAAGTT 
AAAAAACAGT ATGTGTTCCA GATGTCGTCT CTGAATTCTC AAGAGCGGAT TGATTACTGT 
CGCCTGATTA AAGACTTAGG TGGGTCGGTG ATCGAGAAGC AGTGCTCTGA CCCCAGCTGC 
ACACACATGG TTGTGGGCTA CCCACTGCGC AATGAGAAGT ACTTAGCTTC TATGGCAGCT 
GGTAAATGGG TGCTTCATCG CTCCTACCTA GACGCTTGCA AGACAGCTGG GCGCTTTGTG 
CAGGAAGAAG ACTACGAGTG GGGAAGTAGC TCCATACTTG ATGCTCTCCC TGACGTCACT 
GAGCACCAGC AGAAGCTCGC ACTGGCGGCA ATGAGATGGA GGAAAAGAAT CCAGCAAAGT 
CAAGAATCCG GCATTGTTGA GGGAGCATTT AGTGGCTGGA AAGCTATTTT ACGTGTTGAT 
CGGCCCCGAG AAGCTGGGTT CAAACGCCTT CTTCAGGCAG GAGGAGCAAA GGTGTTATCT 
GGTCATCCAG AACCTTTACT TAAAGATGCT ACGCATCTCT TTTGTGACTT CAATAAACTG 
AAACCTGATG ACTGCAGAGT TTTCATCGCA GAGGCTACTG CCCAGAACAT GGTCTGCTTG 
AAAACAGAAT ATATCGCTGA TTATCTCATG CTGGAATCCC CTCCTTGTGC AGACAATTAC 
CGTGTATCCG AAGCTGCTTT ATTCCATAAT AAGAAAGGTG GGCCTGGATT ACCACAGAAG 
AGGAAAACTC CTGCAGAGAA TGTAGTCAAA CGGCCTCGAG TACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_795953.2
CDS	53..4600
Translation	MSRNDQEPFLVKFLKSSDNSECFFKALESIKELQSEDYLQIITDEEALKIRENDKSLYICDRFSGTVFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDVTVSCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYKAETRVEAKMVPDTSTPTAQSNAESHTLADVSHISNINGSCVNETMFGSTTSKLECSLENLENLDISMFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIVGDYDDDVRQFWSKSSHRPHVVGAKWLLECFTKGYILPEESYIHTNYQPAGIAVSDQPGNQTAVLDKSGSFSKSALVPAERLQQADEDLLAQYGNDDSTMVEAKLSEALEPEVGPCPGSAHREPCDDSTHISVQEENKSSVSHCILDDSTVREEGLFSQKSFLVLGFSVENKCNIVDIIREHAGKIVSLPSRIVADYAVVPLLGCEVDVTVGEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGVTPLEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKANAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLETARIGKRADENHFLVDNAPKQEQVLETKIPNGVSSNPDLPAHPDAHLEIHRKKAVTPLDMNRFQSRAFRAVISQQRGQDPTFPPVRQPLTKEPSLHLDTPSKFLSKDKLFKPSFDVTDALAALETPNAASQKRKLSSPLSEVIVRNLTVALANSSRNTDSHSASPQLKGAHLEEEETRKPLDSVVVCVSKKLSKKQSELNGVAASLGAEYRWSFDETVTHFIYQGRANDSNREYKSAKERGVHIVSEHWLLECAQEYKHLPESLYPHTYNPKMSLDINTVQDGRLCNSRAPLAVSASKDDGPDHLSVEGNETNTMGTNDKESPLLNGSGRDDCKGALTQALEMRENFQKQLQEIMSATCIVKTPAQKTCMSRSSCNSASSTPDSARSVRSGRSRVLEALRQSRQAVPDVNTEPSQNEQIIWDDPTAREERARLASNLQWPSDPTQHSELQVEIKMPDDSPSRKPVYHSEIAEQASCVTQAPGHPGSEEPEPPVAERPLIPEPQAPAVASPLAKPPVAPQPADKIETQEETHRKVKKQYVFQMSSLNSQERIDYCRLIKDLGGSVIEKQCSDPSCTHMVVGYPLRNEKYLASMAAGKWVLHRSYLDACKTAGRFVQEEDYEWGSSSILDALPDVTEHQQKLALAAMRWRKRIQQSQESGIVEGAFSGWKAILRVDRPREAGFKRLLQAGGAKVLSGHPEPLLKDATHLFCDFNKLKPDDCRVFIAEATAQNMVCLKTEYIADYLMLESPPCADNYRVSEAALFHNKKGGPGLPQKRKTPAENVVKRPRVH

Target ORF information:

RefSeq Version	NM_176979.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176979.5

ORF Insert Sequence:

ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG 
ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT 
CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA 
GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA 
CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT 
AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA 
CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT 
ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT 
GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT 
GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT 
GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC 
TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT 
AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG 
GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA 
GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC 
TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG 
ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG 
CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT 
GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC 
CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC 
AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT 
TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG 
CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA 
CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT 
ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC 
TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG 
GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA 
TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG 
AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC 
ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG 
GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT 
TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG 
GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG 
CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT 
TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT 
GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA 
CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT 
TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG 
GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG 
AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC 
GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT 
AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGTGTGCT 
CAAGAGTATA AGCATCTTCC TGAATCTCTT TATCCACACA CTTACAATCC CAAAATGAGT 
TTGGACATCA ACACAGTGCA AGATGGGCGG CTCTGTAACA GCCGGGCACC CTTGGCAGTT 
TCTGCATCTA AGGATGATGG GCCAGATCAT CTGTCTGTAG AAGGAAATGA GACAAACACT 
ATGGGCACAA ATGACAAAGA GTCACCTCTG TTGAATGGGA GTGGAAGAGA TGACTGTAAA 
GGAGCTCTGA CCCAGGCCTT GGAGATGAGA GAGAACTTCC AGAAGCAGCT GCAAGAGATC 
ATGTCAGCCA CCTGCATTGT GAAGACGCCT GCCCAGAAGA CTTGCATGTC AAGAAGCAGC 
TGTAACAGCG CCTCTTCAAC TCCTGACAGC GCTCGCTCTG TGCGGAGCGG ACGAAGCCGA 
GTCCTGGAGG CACTCAGGCA GTCTCGGCAG GCAGTGCCTG ATGTGAACAC GGAGCCGTCC 
CAGAACGAGC AGATCATTTG GGACGACCCG ACAGCAAGGG AAGAGAGAGC AAGGCTTGCC 
AGCAACTTAC AGTGGCCCAG CGACCCCACA CAGCATTCTG AGCTTCAGGT TGAGATAAAA 
ATGCCGGATG ACTCTCCCTC TCGAAAGCCT GTGTACCATT CAGAAATTGC TGAGCAGGCC 
TCGTGTGTTA CTCAAGCCCC AGGGCACCCA GGCTCTGAAG AACCAGAGCC TCCAGTAGCA 
GAAAGGCCTT TGATCCCCGA GCCGCAAGCC CCCGCTGTAG CTTCCCCCCT GGCTAAGCCG 
CCTGTGGCCC CACAACCTGC AGACAAGATT GAAACACAGG AGGAGACGCA CAGAAAAGTT 
AAAAAACAGT ATGTGTTCCA GATGTCGTCT CTGAATTCTC AAGAGCGGAT TGATTACTGT 
CGCCTGATTA AAGACTTAGG TGGGTCGGTG ATCGAGAAGC AGTGCTCTGA CCCCAGCTGC 
ACACACATGG TTGTGGGCTA CCCACTGCGC AATGAGAAGT ACTTAGCTTC TATGGCAGCT 
GGTAAATGGG TGCTTCATCG CTCCTACCTA GACGCTTGCA AGACAGCTGG GCGCTTTGTG 
CAGGAAGAAG ACTACGAGTG GGGAAGTAGC TCCATACTTG ATGCTCTCCC TGACGTCACT 
GAGCACCAGC AGAAGCTCGC ACTGGCGGCA ATGAGATGGA GGAAAAGAAT CCAGCAAAGT 
CAAGAATCCG GCATTGTTGA GGGAGCATTT AGTGGCTGGA AAGCTATTTT ACGTGTTGAT 
CGGCCCCGAG AAGCTGGGTT CAAACGCCTT CTTCAGGCAG GAGGAGCAAA GGTGTTATCT 
GGTCATCCAG AACCTTTACT TAAAGATGCT ACGCATCTCT TTTGTGACTT CAATAAACTG 
AAACCTGATG ACTGCAGAGT TTTCATCGCA GAGGCTACTG CCCAGAACAT GGTCTGCTTG 
AAAACAGAAT ATATCGCTGA TTATCTCATG CTGGAATCCC CTCCTTGTGC AGACAATTAC 
CGTGTATCCG AAGCTGCTTT ATTCCATAAT AAGAAAGGTG GGCCTGGATT ACCACAGAAG 
AGGAAAACTC CTGCAGAGAA TGTAGTCAAA CGGCCTCGAG TACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Topbp1 ( XM_006511707.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511770.1 Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA.

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CloneID	OMu19740
Clone ID Related Accession (Same CDS sequence)	XM_006511707.3 , XM_006511707.4 , NM_176979.5
Accession Version	XM_006511707.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA topoisomerase 2-binding protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511707.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG 
ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT 
CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA 
GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA 
CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT 
AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA 
CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT 
ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT 
GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT 
GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT 
GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC 
TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT 
AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG 
GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA 
GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC 
TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG 
ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG 
CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT 
GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC 
CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC 
AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT 
TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG 
CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA 
CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT 
ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC 
TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG 
GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA 
TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG 
AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC 
ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG 
GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT 
TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG 
GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG 
CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT 
TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT 
GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA 
CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT 
TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG 
GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG 
AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC 
GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT 
AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGTGTGCT 
CAAGAGTATA AGCATCTTCC TGAATCTCTT TATCCACACA CTTACAATCC CAAAATGAGT 
TTGGACATCA ACACAGTGCA AGATGGGCGG CTCTGTAACA GCCGGGCACC CTTGGCAGTT 
TCTGCATCTA AGGATGATGG GCCAGATCAT CTGTCTGTAG AAGGAAATGA GACAAACACT 
ATGGGCACAA ATGACAAAGA GTCACCTCTG TTGAATGGGA GTGGAAGAGA TGACTGTAAA 
GGAGCTCTGA CCCAGGCCTT GGAGATGAGA GAGAACTTCC AGAAGCAGCT GCAAGAGATC 
ATGTCAGCCA CCTGCATTGT GAAGACGCCT GCCCAGAAGA CTTGCATGTC AAGAAGCAGC 
TGTAACAGCG CCTCTTCAAC TCCTGACAGC GCTCGCTCTG TGCGGAGCGG ACGAAGCCGA 
GTCCTGGAGG CACTCAGGCA GTCTCGGCAG GCAGTGCCTG ATGTGAACAC GGAGCCGTCC 
CAGAACGAGC AGATCATTTG GGACGACCCG ACAGCAAGGG AAGAGAGAGC AAGGCTTGCC 
AGCAACTTAC AGTGGCCCAG CGACCCCACA CAGCATTCTG AGCTTCAGGT TGAGATAAAA 
ATGCCGGATG ACTCTCCCTC TCGAAAGCCT GTGTACCATT CAGAAATTGC TGAGCAGGCC 
TCGTGTGTTA CTCAAGCCCC AGGGCACCCA GGCTCTGAAG AACCAGAGCC TCCAGTAGCA 
GAAAGGCCTT TGATCCCCGA GCCGCAAGCC CCCGCTGTAG CTTCCCCCCT GGCTAAGCCG 
CCTGTGGCCC CACAACCTGC AGACAAGATT GAAACACAGG AGGAGACGCA CAGAAAAGTT 
AAAAAACAGT ATGTGTTCCA GATGTCGTCT CTGAATTCTC AAGAGCGGAT TGATTACTGT 
CGCCTGATTA AAGACTTAGG TGGGTCGGTG ATCGAGAAGC AGTGCTCTGA CCCCAGCTGC 
ACACACATGG TTGTGGGCTA CCCACTGCGC AATGAGAAGT ACTTAGCTTC TATGGCAGCT 
GGTAAATGGG TGCTTCATCG CTCCTACCTA GACGCTTGCA AGACAGCTGG GCGCTTTGTG 
CAGGAAGAAG ACTACGAGTG GGGAAGTAGC TCCATACTTG ATGCTCTCCC TGACGTCACT 
GAGCACCAGC AGAAGCTCGC ACTGGCGGCA ATGAGATGGA GGAAAAGAAT CCAGCAAAGT 
CAAGAATCCG GCATTGTTGA GGGAGCATTT AGTGGCTGGA AAGCTATTTT ACGTGTTGAT 
CGGCCCCGAG AAGCTGGGTT CAAACGCCTT CTTCAGGCAG GAGGAGCAAA GGTGTTATCT 
GGTCATCCAG AACCTTTACT TAAAGATGCT ACGCATCTCT TTTGTGACTT CAATAAACTG 
AAACCTGATG ACTGCAGAGT TTTCATCGCA GAGGCTACTG CCCAGAACAT GGTCTGCTTG 
AAAACAGAAT ATATCGCTGA TTATCTCATG CTGGAATCCC CTCCTTGTGC AGACAATTAC 
CGTGTATCCG AAGCTGCTTT ATTCCATAAT AAGAAAGGTG GGCCTGGATT ACCACAGAAG 
AGGAAAACTC CTGCAGAGAA TGTAGTCAAA CGGCCTCGAG TACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511770.1
CDS	142..4689
Translation	MSRNDQEPFLVKFLKSSDNSECFFKALESIKELQSEDYLQIITDEEALKIRENDKSLYICDRFSGTVFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDVTVSCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYKAETRVEAKMVPDTSTPTAQSNAESHTLADVSHISNINGSCVNETMFGSTTSKLECSLENLENLDISMFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIVGDYDDDVRQFWSKSSHRPHVVGAKWLLECFTKGYILPEESYIHTNYQPAGIAVSDQPGNQTAVLDKSGSFSKSALVPAERLQQADEDLLAQYGNDDSTMVEAKLSEALEPEVGPCPGSAHREPCDDSTHISVQEENKSSVSHCILDDSTVREEGLFSQKSFLVLGFSVENKCNIVDIIREHAGKIVSLPSRIVADYAVVPLLGCEVDVTVGEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGVTPLEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKANAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLETARIGKRADENHFLVDNAPKQEQVLETKIPNGVSSNPDLPAHPDAHLEIHRKKAVTPLDMNRFQSRAFRAVISQQRGQDPTFPPVRQPLTKEPSLHLDTPSKFLSKDKLFKPSFDVTDALAALETPNAASQKRKLSSPLSEVIVRNLTVALANSSRNTDSHSASPQLKGAHLEEEETRKPLDSVVVCVSKKLSKKQSELNGVAASLGAEYRWSFDETVTHFIYQGRANDSNREYKSAKERGVHIVSEHWLLECAQEYKHLPESLYPHTYNPKMSLDINTVQDGRLCNSRAPLAVSASKDDGPDHLSVEGNETNTMGTNDKESPLLNGSGRDDCKGALTQALEMRENFQKQLQEIMSATCIVKTPAQKTCMSRSSCNSASSTPDSARSVRSGRSRVLEALRQSRQAVPDVNTEPSQNEQIIWDDPTAREERARLASNLQWPSDPTQHSELQVEIKMPDDSPSRKPVYHSEIAEQASCVTQAPGHPGSEEPEPPVAERPLIPEPQAPAVASPLAKPPVAPQPADKIETQEETHRKVKKQYVFQMSSLNSQERIDYCRLIKDLGGSVIEKQCSDPSCTHMVVGYPLRNEKYLASMAAGKWVLHRSYLDACKTAGRFVQEEDYEWGSSSILDALPDVTEHQQKLALAAMRWRKRIQQSQESGIVEGAFSGWKAILRVDRPREAGFKRLLQAGGAKVLSGHPEPLLKDATHLFCDFNKLKPDDCRVFIAEATAQNMVCLKTEYIADYLMLESPPCADNYRVSEAALFHNKKGGPGLPQKRKTPAENVVKRPRVH

Target ORF information:

RefSeq Version	XM_006511707.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511707.3

ORF Insert Sequence:

ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG 
ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT 
CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA 
GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA 
CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT 
AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA 
CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT 
ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT 
GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT 
GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT 
GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC 
TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT 
AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG 
GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA 
GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC 
TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG 
ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG 
CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT 
GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC 
CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC 
AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT 
TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG 
CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA 
CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT 
ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC 
TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG 
GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA 
TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG 
AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC 
ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG 
GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT 
TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG 
GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG 
CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT 
TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT 
GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA 
CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT 
TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG 
GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG 
AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC 
GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT 
AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGTGTGCT 
CAAGAGTATA AGCATCTTCC TGAATCTCTT TATCCACACA CTTACAATCC CAAAATGAGT 
TTGGACATCA ACACAGTGCA AGATGGGCGG CTCTGTAACA GCCGGGCACC CTTGGCAGTT 
TCTGCATCTA AGGATGATGG GCCAGATCAT CTGTCTGTAG AAGGAAATGA GACAAACACT 
ATGGGCACAA ATGACAAAGA GTCACCTCTG TTGAATGGGA GTGGAAGAGA TGACTGTAAA 
GGAGCTCTGA CCCAGGCCTT GGAGATGAGA GAGAACTTCC AGAAGCAGCT GCAAGAGATC 
ATGTCAGCCA CCTGCATTGT GAAGACGCCT GCCCAGAAGA CTTGCATGTC AAGAAGCAGC 
TGTAACAGCG CCTCTTCAAC TCCTGACAGC GCTCGCTCTG TGCGGAGCGG ACGAAGCCGA 
GTCCTGGAGG CACTCAGGCA GTCTCGGCAG GCAGTGCCTG ATGTGAACAC GGAGCCGTCC 
CAGAACGAGC AGATCATTTG GGACGACCCG ACAGCAAGGG AAGAGAGAGC AAGGCTTGCC 
AGCAACTTAC AGTGGCCCAG CGACCCCACA CAGCATTCTG AGCTTCAGGT TGAGATAAAA 
ATGCCGGATG ACTCTCCCTC TCGAAAGCCT GTGTACCATT CAGAAATTGC TGAGCAGGCC 
TCGTGTGTTA CTCAAGCCCC AGGGCACCCA GGCTCTGAAG AACCAGAGCC TCCAGTAGCA 
GAAAGGCCTT TGATCCCCGA GCCGCAAGCC CCCGCTGTAG CTTCCCCCCT GGCTAAGCCG 
CCTGTGGCCC CACAACCTGC AGACAAGATT GAAACACAGG AGGAGACGCA CAGAAAAGTT 
AAAAAACAGT ATGTGTTCCA GATGTCGTCT CTGAATTCTC AAGAGCGGAT TGATTACTGT 
CGCCTGATTA AAGACTTAGG TGGGTCGGTG ATCGAGAAGC AGTGCTCTGA CCCCAGCTGC 
ACACACATGG TTGTGGGCTA CCCACTGCGC AATGAGAAGT ACTTAGCTTC TATGGCAGCT 
GGTAAATGGG TGCTTCATCG CTCCTACCTA GACGCTTGCA AGACAGCTGG GCGCTTTGTG 
CAGGAAGAAG ACTACGAGTG GGGAAGTAGC TCCATACTTG ATGCTCTCCC TGACGTCACT 
GAGCACCAGC AGAAGCTCGC ACTGGCGGCA ATGAGATGGA GGAAAAGAAT CCAGCAAAGT 
CAAGAATCCG GCATTGTTGA GGGAGCATTT AGTGGCTGGA AAGCTATTTT ACGTGTTGAT 
CGGCCCCGAG AAGCTGGGTT CAAACGCCTT CTTCAGGCAG GAGGAGCAAA GGTGTTATCT 
GGTCATCCAG AACCTTTACT TAAAGATGCT ACGCATCTCT TTTGTGACTT CAATAAACTG 
AAACCTGATG ACTGCAGAGT TTTCATCGCA GAGGCTACTG CCCAGAACAT GGTCTGCTTG 
AAAACAGAAT ATATCGCTGA TTATCTCATG CTGGAATCCC CTCCTTGTGC AGACAATTAC 
CGTGTATCCG AAGCTGCTTT ATTCCATAAT AAGAAAGGTG GGCCTGGATT ACCACAGAAG 
AGGAAAACTC CTGCAGAGAA TGTAGTCAAA CGGCCTCGAG TACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Topbp1 ( XM_006511706.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511769.2 Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.

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CloneID	OMu73844
Clone ID Related Accession (Same CDS sequence)	XM_006511706.4 , XM_006511706.3
Accession Version	XM_006511706.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA topoisomerase 2-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG 
ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT 
CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA 
GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA 
CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT 
AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA 
CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT 
ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT 
GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT 
GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT 
GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC 
TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT 
AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG 
GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA 
GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC 
TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG 
ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG 
CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT 
GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC 
CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC 
AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT 
TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG 
CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA 
CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT 
ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC 
TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG 
GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA 
TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG 
AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC 
ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG 
GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT 
TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG 
GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG 
CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT 
TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT 
GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA 
CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT 
TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG 
GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG 
AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC 
GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT 
AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGCCAGAT 
CATCTGTCTG TAGAAGGAAA TGAGACAAAC ACTATGGGCA CAAATGACAA AGAGTCACCT 
CTGTTGAATG GGAGTGGAAG AGATGACTGT AAAGGAGCTC TGACCCAGGC CTTGGAGATG 
AGAGAGAACT TCCAGAAGCA GCTGCAAGAG ATCATGTCAG CCACCTGCAT TGTGAAGACG 
CCTGCCCAGA AGACTTGCAT GTCAAGAAGC AGCTGTAACA GCGCCTCTTC AACTCCTGAC 
AGCGCTCGCT CTGTGCGGAG CGGACGAAGC CGAGTCCTGG AGGCACTCAG GCAGTCTCGG 
CAGGCAGTGC CTGATGTGAA CACGGAGCCG TCCCAGAACG AGCAGATCAT TTGGGACGAC 
CCGACAGCAA GGGAAGAGAG AGCAAGGCTT GCCAGCAACT TACAGTGGCC CAGCGACCCC 
ACACAGCATT CTGAGCTTCA GGTTGAGATA AAAATGCCGG ATGACTCTCC CTCTCGAAAG 
CCTGTGTACC ATTCAGAAAT TGCTGAGCAG GCCTCGTGTG TTACTCAAGC CCCAGGGCAC 
CCAGGCTCTG AAGAACCAGA GCCTCCAGTA GCAGAAAGGC CTTTGATCCC CGAGCCGCAA 
GCCCCCGCTG TAGCTTCCCC CCTGGCTAAG CCGCCTGTGG CCCCACAACC TGCAGACAAG 
ATTGAAACAC AGGAGGAGAC GCACAGAAAA GTTAAAAAAC AGTATGTGTT CCAGATGTCG 
TCTCTGAATT CTCAAGAGCG GATTGATTAC TGTCGCCTGA TTAAAGACTT AGGTGGGTCG 
GTGATCGAGA AGCAGTGCTC TGACCCCAGC TGCACACACA TGGTTGTGGG CTACCCACTG 
CGCAATGAGA AGTACTTAGC TTCTATGGCA GCTGGTAAAT GGGTGCTTCA TCGCTCCTAC 
CTAGACGCTT GCAAGACAGC TGGGCGCTTT GTGCAGGAAG AAGACTACGA GTGGGGAAGT 
AGCTCCATAC TTGATGCTCT CCCTGACGTC ACTGAGCACC AGCAGAAGCT CGCACTGGCG 
GCAATGAGAT GGAGGAAAAG AATCCAGCAA AGTCAAGAAT CCGGCATTGT TGAGGGAGCA 
TTTAGTGGCT GGAAAGCTAT TTTACGTGTT GATCGGCCCC GAGAAGCTGG GTTCAAACGC 
CTTCTTCAGG CAGGAGGAGC AAAGGTGTTA TCTGGTCATC CAGAACCTTT ACTTAAAGAT 
GCTACGCATC TCTTTTGTGA CTTCAATAAA CTGAAACCTG ATGACTGCAG AGTTTTCATC 
GCAGAGGCTA CTGCCCAGAA CATGGTCTGC TTGAAAACAG AATATATCGC TGATTATCTC 
ATGCTGGAAT CCCCTCCTTG TGCAGACAAT TACCGTGTAT CCGAAGCTGC TTTATTCCAT 
AATAAGAAAG GTGGGCCTGG ATTACCACAG AAGAGGAAAA CTCCTGCAGA GAATGTAGTC 
AAACGGCCTC GAGTACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511769.2
CDS	212..4612
Translation	MSRNDQEPFLVKFLKSSDNSECFFKALESIKELQSEDYLQIITDEEALKIRENDKSLYICDRFSGTVFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDVTVSCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYKAETRVEAKMVPDTSTPTAQSNAESHTLADVSHISNINGSCVNETMFGSTTSKLECSLENLENLDISMFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIVGDYDDDVRQFWSKSSHRPHVVGAKWLLECFTKGYILPEESYIHTNYQPAGIAVSDQPGNQTAVLDKSGSFSKSALVPAERLQQADEDLLAQYGNDDSTMVEAKLSEALEPEVGPCPGSAHREPCDDSTHISVQEENKSSVSHCILDDSTVREEGLFSQKSFLVLGFSVENKCNIVDIIREHAGKIVSLPSRIVADYAVVPLLGCEVDVTVGEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGVTPLEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKANAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLETARIGKRADENHFLVDNAPKQEQVLETKIPNGVSSNPDLPAHPDAHLEIHRKKAVTPLDMNRFQSRAFRAVISQQRGQDPTFPPVRQPLTKEPSLHLDTPSKFLSKDKLFKPSFDVTDALAALETPNAASQKRKLSSPLSEVIVRNLTVALANSSRNTDSHSASPQLKGAHLEEEETRKPLDSVVVCVSKKLSKKQSELNGVAASLGAEYRWSFDETVTHFIYQGRANDSNREYKSAKERGVHIVSEHWLLEPDHLSVEGNETNTMGTNDKESPLLNGSGRDDCKGALTQALEMRENFQKQLQEIMSATCIVKTPAQKTCMSRSSCNSASSTPDSARSVRSGRSRVLEALRQSRQAVPDVNTEPSQNEQIIWDDPTAREERARLASNLQWPSDPTQHSELQVEIKMPDDSPSRKPVYHSEIAEQASCVTQAPGHPGSEEPEPPVAERPLIPEPQAPAVASPLAKPPVAPQPADKIETQEETHRKVKKQYVFQMSSLNSQERIDYCRLIKDLGGSVIEKQCSDPSCTHMVVGYPLRNEKYLASMAAGKWVLHRSYLDACKTAGRFVQEEDYEWGSSSILDALPDVTEHQQKLALAAMRWRKRIQQSQESGIVEGAFSGWKAILRVDRPREAGFKRLLQAGGAKVLSGHPEPLLKDATHLFCDFNKLKPDDCRVFIAEATAQNMVCLKTEYIADYLMLESPPCADNYRVSEAALFHNKKGGPGLPQKRKTPAENVVKRPRVH

Target ORF information:

RefSeq Version	XM_006511706.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511706.3

ORF Insert Sequence:

ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG 
ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT 
CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA 
GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA 
CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT 
AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA 
CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT 
ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT 
GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT 
GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT 
GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC 
TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT 
AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG 
GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA 
GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC 
TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG 
ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG 
CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT 
GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC 
CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC 
AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT 
TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG 
CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA 
CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT 
ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC 
TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG 
GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA 
TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG 
AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC 
ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG 
GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT 
TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG 
GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG 
CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT 
TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT 
GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA 
CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT 
TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG 
GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG 
AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC 
GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT 
AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGCCAGAT 
CATCTGTCTG TAGAAGGAAA TGAGACAAAC ACTATGGGCA CAAATGACAA AGAGTCACCT 
CTGTTGAATG GGAGTGGAAG AGATGACTGT AAAGGAGCTC TGACCCAGGC CTTGGAGATG 
AGAGAGAACT TCCAGAAGCA GCTGCAAGAG ATCATGTCAG CCACCTGCAT TGTGAAGACG 
CCTGCCCAGA AGACTTGCAT GTCAAGAAGC AGCTGTAACA GCGCCTCTTC AACTCCTGAC 
AGCGCTCGCT CTGTGCGGAG CGGACGAAGC CGAGTCCTGG AGGCACTCAG GCAGTCTCGG 
CAGGCAGTGC CTGATGTGAA CACGGAGCCG TCCCAGAACG AGCAGATCAT TTGGGACGAC 
CCGACAGCAA GGGAAGAGAG AGCAAGGCTT GCCAGCAACT TACAGTGGCC CAGCGACCCC 
ACACAGCATT CTGAGCTTCA GGTTGAGATA AAAATGCCGG ATGACTCTCC CTCTCGAAAG 
CCTGTGTACC ATTCAGAAAT TGCTGAGCAG GCCTCGTGTG TTACTCAAGC CCCAGGGCAC 
CCAGGCTCTG AAGAACCAGA GCCTCCAGTA GCAGAAAGGC CTTTGATCCC CGAGCCGCAA 
GCCCCCGCTG TAGCTTCCCC CCTGGCTAAG CCGCCTGTGG CCCCACAACC TGCAGACAAG 
ATTGAAACAC AGGAGGAGAC GCACAGAAAA GTTAAAAAAC AGTATGTGTT CCAGATGTCG 
TCTCTGAATT CTCAAGAGCG GATTGATTAC TGTCGCCTGA TTAAAGACTT AGGTGGGTCG 
GTGATCGAGA AGCAGTGCTC TGACCCCAGC TGCACACACA TGGTTGTGGG CTACCCACTG 
CGCAATGAGA AGTACTTAGC TTCTATGGCA GCTGGTAAAT GGGTGCTTCA TCGCTCCTAC 
CTAGACGCTT GCAAGACAGC TGGGCGCTTT GTGCAGGAAG AAGACTACGA GTGGGGAAGT 
AGCTCCATAC TTGATGCTCT CCCTGACGTC ACTGAGCACC AGCAGAAGCT CGCACTGGCG 
GCAATGAGAT GGAGGAAAAG AATCCAGCAA AGTCAAGAAT CCGGCATTGT TGAGGGAGCA 
TTTAGTGGCT GGAAAGCTAT TTTACGTGTT GATCGGCCCC GAGAAGCTGG GTTCAAACGC 
CTTCTTCAGG CAGGAGGAGC AAAGGTGTTA TCTGGTCATC CAGAACCTTT ACTTAAAGAT 
GCTACGCATC TCTTTTGTGA CTTCAATAAA CTGAAACCTG ATGACTGCAG AGTTTTCATC 
GCAGAGGCTA CTGCCCAGAA CATGGTCTGC TTGAAAACAG AATATATCGC TGATTATCTC 
ATGCTGGAAT CCCCTCCTTG TGCAGACAAT TACCGTGTAT CCGAAGCTGC TTTATTCCAT 
AATAAGAAAG GTGGGCCTGG ATTACCACAG AAGAGGAAAA CTCCTGCAGA GAATGTAGTC 
AAACGGCCTC GAGTACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNA damage response protein TOPBP1 regulates X chromosome silencing in the mammalian germ line.
Proceedings of the National Academy of Sciences of the United States of America114(47)12536-12541(2017 Nov)
ElInati E,Russell HR,Ojarikre OA,Sangrithi M,Hirota T,de Rooij DG,McKinnon PJ,Turner JMA

Elucidation of the Fanconi Anemia Protein Network in Meiosis and Its Function in the Regulation of Histone Modifications.
Cell reports17(4)1141-1157(2016 10)
Alavattam KG,Kato Y,Sin HS,Maezawa S,Kowalski IJ,Zhang F,Pang Q,Andreassen PR,Namekawa SH

Levels of the E2 interacting protein TopBP1 modulate papillomavirus maintenance stage replication.
Virology478129-35(2015 Apr)
Kanginakudru S,DeSmet M,Thomas Y,Morgan IM,Androphy EJ

SCML2 establishes the male germline epigenome through regulation of histone H2A ubiquitination.
Developmental cell32(5)574-88(2015 Mar)
Hasegawa K,Sin HS,Maezawa S,Broering TJ,Kartashov AV,Alavattam KG,Ichijima Y,Zhang F,Bacon WC,Greis KD,Andreassen PR,Barski A,Namekawa SH

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Topbp1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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