Topbp1 cDNA ORF clone, Mus musculus(house mouse)

The following Topbp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Topbp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu73844 XM_006511706.4
Latest version!
Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu19740 XM_006511707.4
Latest version!
Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu19740 NM_176979.5
Latest version!
Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
View Old Accession Versions >>
OMu73844 XM_006511706.3 Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu19740 XM_006511707.3 Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu73844
    Clone ID Related Accession (Same CDS sequence) XM_006511706.4 , XM_006511706.3
    Accession Version XM_006511706.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511706.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG
    ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT
    CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA
    GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA
    CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT
    AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA
    CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT
    ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT
    GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT
    GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT
    GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC
    TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT
    AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG
    GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA
    GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC
    TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG
    ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG
    CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT
    GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC
    CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC
    AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT
    TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG
    CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA
    CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT
    ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC
    TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG
    GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA
    TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG
    AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC
    ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG
    GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT
    TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG
    GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG
    CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT
    TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT
    GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA
    CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT
    TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG
    GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG
    AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC
    GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT
    AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGCCAGAT
    CATCTGTCTG TAGAAGGAAA TGAGACAAAC ACTATGGGCA CAAATGACAA AGAGTCACCT
    CTGTTGAATG GGAGTGGAAG AGATGACTGT AAAGGAGCTC TGACCCAGGC CTTGGAGATG
    AGAGAGAACT TCCAGAAGCA GCTGCAAGAG ATCATGTCAG CCACCTGCAT TGTGAAGACG
    CCTGCCCAGA AGACTTGCAT GTCAAGAAGC AGCTGTAACA GCGCCTCTTC AACTCCTGAC
    AGCGCTCGCT CTGTGCGGAG CGGACGAAGC CGAGTCCTGG AGGCACTCAG GCAGTCTCGG
    CAGGCAGTGC CTGATGTGAA CACGGAGCCG TCCCAGAACG AGCAGATCAT TTGGGACGAC
    CCGACAGCAA GGGAAGAGAG AGCAAGGCTT GCCAGCAACT TACAGTGGCC CAGCGACCCC
    ACACAGCATT CTGAGCTTCA GGTTGAGATA AAAATGCCGG ATGACTCTCC CTCTCGAAAG
    CCTGTGTACC ATTCAGAAAT TGCTGAGCAG GCCTCGTGTG TTACTCAAGC CCCAGGGCAC
    CCAGGCTCTG AAGAACCAGA GCCTCCAGTA GCAGAAAGGC CTTTGATCCC CGAGCCGCAA
    GCCCCCGCTG TAGCTTCCCC CCTGGCTAAG CCGCCTGTGG CCCCACAACC TGCAGACAAG
    ATTGAAACAC AGGAGGAGAC GCACAGAAAA GTTAAAAAAC AGTATGTGTT CCAGATGTCG
    TCTCTGAATT CTCAAGAGCG GATTGATTAC TGTCGCCTGA TTAAAGACTT AGGTGGGTCG
    GTGATCGAGA AGCAGTGCTC TGACCCCAGC TGCACACACA TGGTTGTGGG CTACCCACTG
    CGCAATGAGA AGTACTTAGC TTCTATGGCA GCTGGTAAAT GGGTGCTTCA TCGCTCCTAC
    CTAGACGCTT GCAAGACAGC TGGGCGCTTT GTGCAGGAAG AAGACTACGA GTGGGGAAGT
    AGCTCCATAC TTGATGCTCT CCCTGACGTC ACTGAGCACC AGCAGAAGCT CGCACTGGCG
    GCAATGAGAT GGAGGAAAAG AATCCAGCAA AGTCAAGAAT CCGGCATTGT TGAGGGAGCA
    TTTAGTGGCT GGAAAGCTAT TTTACGTGTT GATCGGCCCC GAGAAGCTGG GTTCAAACGC
    CTTCTTCAGG CAGGAGGAGC AAAGGTGTTA TCTGGTCATC CAGAACCTTT ACTTAAAGAT
    GCTACGCATC TCTTTTGTGA CTTCAATAAA CTGAAACCTG ATGACTGCAG AGTTTTCATC
    GCAGAGGCTA CTGCCCAGAA CATGGTCTGC TTGAAAACAG AATATATCGC TGATTATCTC
    ATGCTGGAAT CCCCTCCTTG TGCAGACAAT TACCGTGTAT CCGAAGCTGC TTTATTCCAT
    AATAAGAAAG GTGGGCCTGG ATTACCACAG AAGAGGAAAA CTCCTGCAGA GAATGTAGTC
    AAACGGCCTC GAGTACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006511769.2
    CDS213..4613
    Translation

    Target ORF information:

    RefSeq Version XM_006511706.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006511706.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG
    ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT
    CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA
    GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA
    CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT
    AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA
    CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT
    ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT
    GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT
    GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT
    GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC
    TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT
    AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG
    GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA
    GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC
    TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG
    ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG
    CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT
    GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC
    CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC
    AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT
    TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG
    CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA
    CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT
    ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC
    TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG
    GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA
    TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG
    AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC
    ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG
    GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT
    TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG
    GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG
    CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT
    TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT
    GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA
    CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT
    TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG
    GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG
    AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC
    GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT
    AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGCCAGAT
    CATCTGTCTG TAGAAGGAAA TGAGACAAAC ACTATGGGCA CAAATGACAA AGAGTCACCT
    CTGTTGAATG GGAGTGGAAG AGATGACTGT AAAGGAGCTC TGACCCAGGC CTTGGAGATG
    AGAGAGAACT TCCAGAAGCA GCTGCAAGAG ATCATGTCAG CCACCTGCAT TGTGAAGACG
    CCTGCCCAGA AGACTTGCAT GTCAAGAAGC AGCTGTAACA GCGCCTCTTC AACTCCTGAC
    AGCGCTCGCT CTGTGCGGAG CGGACGAAGC CGAGTCCTGG AGGCACTCAG GCAGTCTCGG
    CAGGCAGTGC CTGATGTGAA CACGGAGCCG TCCCAGAACG AGCAGATCAT TTGGGACGAC
    CCGACAGCAA GGGAAGAGAG AGCAAGGCTT GCCAGCAACT TACAGTGGCC CAGCGACCCC
    ACACAGCATT CTGAGCTTCA GGTTGAGATA AAAATGCCGG ATGACTCTCC CTCTCGAAAG
    CCTGTGTACC ATTCAGAAAT TGCTGAGCAG GCCTCGTGTG TTACTCAAGC CCCAGGGCAC
    CCAGGCTCTG AAGAACCAGA GCCTCCAGTA GCAGAAAGGC CTTTGATCCC CGAGCCGCAA
    GCCCCCGCTG TAGCTTCCCC CCTGGCTAAG CCGCCTGTGG CCCCACAACC TGCAGACAAG
    ATTGAAACAC AGGAGGAGAC GCACAGAAAA GTTAAAAAAC AGTATGTGTT CCAGATGTCG
    TCTCTGAATT CTCAAGAGCG GATTGATTAC TGTCGCCTGA TTAAAGACTT AGGTGGGTCG
    GTGATCGAGA AGCAGTGCTC TGACCCCAGC TGCACACACA TGGTTGTGGG CTACCCACTG
    CGCAATGAGA AGTACTTAGC TTCTATGGCA GCTGGTAAAT GGGTGCTTCA TCGCTCCTAC
    CTAGACGCTT GCAAGACAGC TGGGCGCTTT GTGCAGGAAG AAGACTACGA GTGGGGAAGT
    AGCTCCATAC TTGATGCTCT CCCTGACGTC ACTGAGCACC AGCAGAAGCT CGCACTGGCG
    GCAATGAGAT GGAGGAAAAG AATCCAGCAA AGTCAAGAAT CCGGCATTGT TGAGGGAGCA
    TTTAGTGGCT GGAAAGCTAT TTTACGTGTT GATCGGCCCC GAGAAGCTGG GTTCAAACGC
    CTTCTTCAGG CAGGAGGAGC AAAGGTGTTA TCTGGTCATC CAGAACCTTT ACTTAAAGAT
    GCTACGCATC TCTTTTGTGA CTTCAATAAA CTGAAACCTG ATGACTGCAG AGTTTTCATC
    GCAGAGGCTA CTGCCCAGAA CATGGTCTGC TTGAAAACAG AATATATCGC TGATTATCTC
    ATGCTGGAAT CCCCTCCTTG TGCAGACAAT TACCGTGTAT CCGAAGCTGC TTTATTCCAT
    AATAAGAAAG GTGGGCCTGG ATTACCACAG AAGAGGAAAA CTCCTGCAGA GAATGTAGTC
    AAACGGCCTC GAGTACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu19740
    Clone ID Related Accession (Same CDS sequence) XM_006511707.3 , XM_006511707.4 , NM_176979.5
    Accession Version XM_006511707.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product DNA topoisomerase 2-binding protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511707.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG
    ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT
    CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA
    GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA
    CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT
    AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA
    CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT
    ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT
    GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT
    GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT
    GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC
    TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT
    AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG
    GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA
    GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC
    TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG
    ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG
    CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT
    GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC
    CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC
    AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT
    TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG
    CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA
    CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT
    ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC
    TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG
    GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA
    TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG
    AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC
    ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG
    GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT
    TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG
    GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG
    CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT
    TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT
    GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA
    CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT
    TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG
    GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG
    AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC
    GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT
    AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGTGTGCT
    CAAGAGTATA AGCATCTTCC TGAATCTCTT TATCCACACA CTTACAATCC CAAAATGAGT
    TTGGACATCA ACACAGTGCA AGATGGGCGG CTCTGTAACA GCCGGGCACC CTTGGCAGTT
    TCTGCATCTA AGGATGATGG GCCAGATCAT CTGTCTGTAG AAGGAAATGA GACAAACACT
    ATGGGCACAA ATGACAAAGA GTCACCTCTG TTGAATGGGA GTGGAAGAGA TGACTGTAAA
    GGAGCTCTGA CCCAGGCCTT GGAGATGAGA GAGAACTTCC AGAAGCAGCT GCAAGAGATC
    ATGTCAGCCA CCTGCATTGT GAAGACGCCT GCCCAGAAGA CTTGCATGTC AAGAAGCAGC
    TGTAACAGCG CCTCTTCAAC TCCTGACAGC GCTCGCTCTG TGCGGAGCGG ACGAAGCCGA
    GTCCTGGAGG CACTCAGGCA GTCTCGGCAG GCAGTGCCTG ATGTGAACAC GGAGCCGTCC
    CAGAACGAGC AGATCATTTG GGACGACCCG ACAGCAAGGG AAGAGAGAGC AAGGCTTGCC
    AGCAACTTAC AGTGGCCCAG CGACCCCACA CAGCATTCTG AGCTTCAGGT TGAGATAAAA
    ATGCCGGATG ACTCTCCCTC TCGAAAGCCT GTGTACCATT CAGAAATTGC TGAGCAGGCC
    TCGTGTGTTA CTCAAGCCCC AGGGCACCCA GGCTCTGAAG AACCAGAGCC TCCAGTAGCA
    GAAAGGCCTT TGATCCCCGA GCCGCAAGCC CCCGCTGTAG CTTCCCCCCT GGCTAAGCCG
    CCTGTGGCCC CACAACCTGC AGACAAGATT GAAACACAGG AGGAGACGCA CAGAAAAGTT
    AAAAAACAGT ATGTGTTCCA GATGTCGTCT CTGAATTCTC AAGAGCGGAT TGATTACTGT
    CGCCTGATTA AAGACTTAGG TGGGTCGGTG ATCGAGAAGC AGTGCTCTGA CCCCAGCTGC
    ACACACATGG TTGTGGGCTA CCCACTGCGC AATGAGAAGT ACTTAGCTTC TATGGCAGCT
    GGTAAATGGG TGCTTCATCG CTCCTACCTA GACGCTTGCA AGACAGCTGG GCGCTTTGTG
    CAGGAAGAAG ACTACGAGTG GGGAAGTAGC TCCATACTTG ATGCTCTCCC TGACGTCACT
    GAGCACCAGC AGAAGCTCGC ACTGGCGGCA ATGAGATGGA GGAAAAGAAT CCAGCAAAGT
    CAAGAATCCG GCATTGTTGA GGGAGCATTT AGTGGCTGGA AAGCTATTTT ACGTGTTGAT
    CGGCCCCGAG AAGCTGGGTT CAAACGCCTT CTTCAGGCAG GAGGAGCAAA GGTGTTATCT
    GGTCATCCAG AACCTTTACT TAAAGATGCT ACGCATCTCT TTTGTGACTT CAATAAACTG
    AAACCTGATG ACTGCAGAGT TTTCATCGCA GAGGCTACTG CCCAGAACAT GGTCTGCTTG
    AAAACAGAAT ATATCGCTGA TTATCTCATG CTGGAATCCC CTCCTTGTGC AGACAATTAC
    CGTGTATCCG AAGCTGCTTT ATTCCATAAT AAGAAAGGTG GGCCTGGATT ACCACAGAAG
    AGGAAAACTC CTGCAGAGAA TGTAGTCAAA CGGCCTCGAG TACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006511770.1
    CDS243..4790
    Translation

    Target ORF information:

    RefSeq Version XM_006511707.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006511707.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG
    ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT
    CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA
    GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA
    CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT
    AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA
    CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT
    ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT
    GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT
    GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT
    GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC
    TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT
    AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG
    GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA
    GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC
    TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG
    ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG
    CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT
    GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC
    CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC
    AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT
    TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG
    CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA
    CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT
    ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC
    TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG
    GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA
    TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG
    AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC
    ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG
    GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT
    TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG
    GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG
    CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT
    TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT
    GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA
    CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT
    TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG
    GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG
    AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC
    GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT
    AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGTGTGCT
    CAAGAGTATA AGCATCTTCC TGAATCTCTT TATCCACACA CTTACAATCC CAAAATGAGT
    TTGGACATCA ACACAGTGCA AGATGGGCGG CTCTGTAACA GCCGGGCACC CTTGGCAGTT
    TCTGCATCTA AGGATGATGG GCCAGATCAT CTGTCTGTAG AAGGAAATGA GACAAACACT
    ATGGGCACAA ATGACAAAGA GTCACCTCTG TTGAATGGGA GTGGAAGAGA TGACTGTAAA
    GGAGCTCTGA CCCAGGCCTT GGAGATGAGA GAGAACTTCC AGAAGCAGCT GCAAGAGATC
    ATGTCAGCCA CCTGCATTGT GAAGACGCCT GCCCAGAAGA CTTGCATGTC AAGAAGCAGC
    TGTAACAGCG CCTCTTCAAC TCCTGACAGC GCTCGCTCTG TGCGGAGCGG ACGAAGCCGA
    GTCCTGGAGG CACTCAGGCA GTCTCGGCAG GCAGTGCCTG ATGTGAACAC GGAGCCGTCC
    CAGAACGAGC AGATCATTTG GGACGACCCG ACAGCAAGGG AAGAGAGAGC AAGGCTTGCC
    AGCAACTTAC AGTGGCCCAG CGACCCCACA CAGCATTCTG AGCTTCAGGT TGAGATAAAA
    ATGCCGGATG ACTCTCCCTC TCGAAAGCCT GTGTACCATT CAGAAATTGC TGAGCAGGCC
    TCGTGTGTTA CTCAAGCCCC AGGGCACCCA GGCTCTGAAG AACCAGAGCC TCCAGTAGCA
    GAAAGGCCTT TGATCCCCGA GCCGCAAGCC CCCGCTGTAG CTTCCCCCCT GGCTAAGCCG
    CCTGTGGCCC CACAACCTGC AGACAAGATT GAAACACAGG AGGAGACGCA CAGAAAAGTT
    AAAAAACAGT ATGTGTTCCA GATGTCGTCT CTGAATTCTC AAGAGCGGAT TGATTACTGT
    CGCCTGATTA AAGACTTAGG TGGGTCGGTG ATCGAGAAGC AGTGCTCTGA CCCCAGCTGC
    ACACACATGG TTGTGGGCTA CCCACTGCGC AATGAGAAGT ACTTAGCTTC TATGGCAGCT
    GGTAAATGGG TGCTTCATCG CTCCTACCTA GACGCTTGCA AGACAGCTGG GCGCTTTGTG
    CAGGAAGAAG ACTACGAGTG GGGAAGTAGC TCCATACTTG ATGCTCTCCC TGACGTCACT
    GAGCACCAGC AGAAGCTCGC ACTGGCGGCA ATGAGATGGA GGAAAAGAAT CCAGCAAAGT
    CAAGAATCCG GCATTGTTGA GGGAGCATTT AGTGGCTGGA AAGCTATTTT ACGTGTTGAT
    CGGCCCCGAG AAGCTGGGTT CAAACGCCTT CTTCAGGCAG GAGGAGCAAA GGTGTTATCT
    GGTCATCCAG AACCTTTACT TAAAGATGCT ACGCATCTCT TTTGTGACTT CAATAAACTG
    AAACCTGATG ACTGCAGAGT TTTCATCGCA GAGGCTACTG CCCAGAACAT GGTCTGCTTG
    AAAACAGAAT ATATCGCTGA TTATCTCATG CTGGAATCCC CTCCTTGTGC AGACAATTAC
    CGTGTATCCG AAGCTGCTTT ATTCCATAAT AAGAAAGGTG GGCCTGGATT ACCACAGAAG
    AGGAAAACTC CTGCAGAGAA TGTAGTCAAA CGGCCTCGAG TACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu19740
    Clone ID Related Accession (Same CDS sequence) XM_006511707.3 , XM_006511707.4 , NM_176979.5
    Accession Version NM_176979.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-15
    Organism Mus musculus(house mouse)
    Product DNA topoisomerase 2-binding protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC122747.11. On Nov 17, 2006 this sequence version replaced NM_176979.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC049797.1, AK129104.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG
    ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT
    CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA
    GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA
    CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT
    AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA
    CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT
    ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT
    GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT
    GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT
    GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC
    TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT
    AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG
    GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA
    GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC
    TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG
    ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG
    CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT
    GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC
    CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC
    AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT
    TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG
    CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA
    CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT
    ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC
    TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG
    GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA
    TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG
    AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC
    ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG
    GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT
    TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG
    GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG
    CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT
    TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT
    GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA
    CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT
    TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG
    GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG
    AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC
    GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT
    AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGTGTGCT
    CAAGAGTATA AGCATCTTCC TGAATCTCTT TATCCACACA CTTACAATCC CAAAATGAGT
    TTGGACATCA ACACAGTGCA AGATGGGCGG CTCTGTAACA GCCGGGCACC CTTGGCAGTT
    TCTGCATCTA AGGATGATGG GCCAGATCAT CTGTCTGTAG AAGGAAATGA GACAAACACT
    ATGGGCACAA ATGACAAAGA GTCACCTCTG TTGAATGGGA GTGGAAGAGA TGACTGTAAA
    GGAGCTCTGA CCCAGGCCTT GGAGATGAGA GAGAACTTCC AGAAGCAGCT GCAAGAGATC
    ATGTCAGCCA CCTGCATTGT GAAGACGCCT GCCCAGAAGA CTTGCATGTC AAGAAGCAGC
    TGTAACAGCG CCTCTTCAAC TCCTGACAGC GCTCGCTCTG TGCGGAGCGG ACGAAGCCGA
    GTCCTGGAGG CACTCAGGCA GTCTCGGCAG GCAGTGCCTG ATGTGAACAC GGAGCCGTCC
    CAGAACGAGC AGATCATTTG GGACGACCCG ACAGCAAGGG AAGAGAGAGC AAGGCTTGCC
    AGCAACTTAC AGTGGCCCAG CGACCCCACA CAGCATTCTG AGCTTCAGGT TGAGATAAAA
    ATGCCGGATG ACTCTCCCTC TCGAAAGCCT GTGTACCATT CAGAAATTGC TGAGCAGGCC
    TCGTGTGTTA CTCAAGCCCC AGGGCACCCA GGCTCTGAAG AACCAGAGCC TCCAGTAGCA
    GAAAGGCCTT TGATCCCCGA GCCGCAAGCC CCCGCTGTAG CTTCCCCCCT GGCTAAGCCG
    CCTGTGGCCC CACAACCTGC AGACAAGATT GAAACACAGG AGGAGACGCA CAGAAAAGTT
    AAAAAACAGT ATGTGTTCCA GATGTCGTCT CTGAATTCTC AAGAGCGGAT TGATTACTGT
    CGCCTGATTA AAGACTTAGG TGGGTCGGTG ATCGAGAAGC AGTGCTCTGA CCCCAGCTGC
    ACACACATGG TTGTGGGCTA CCCACTGCGC AATGAGAAGT ACTTAGCTTC TATGGCAGCT
    GGTAAATGGG TGCTTCATCG CTCCTACCTA GACGCTTGCA AGACAGCTGG GCGCTTTGTG
    CAGGAAGAAG ACTACGAGTG GGGAAGTAGC TCCATACTTG ATGCTCTCCC TGACGTCACT
    GAGCACCAGC AGAAGCTCGC ACTGGCGGCA ATGAGATGGA GGAAAAGAAT CCAGCAAAGT
    CAAGAATCCG GCATTGTTGA GGGAGCATTT AGTGGCTGGA AAGCTATTTT ACGTGTTGAT
    CGGCCCCGAG AAGCTGGGTT CAAACGCCTT CTTCAGGCAG GAGGAGCAAA GGTGTTATCT
    GGTCATCCAG AACCTTTACT TAAAGATGCT ACGCATCTCT TTTGTGACTT CAATAAACTG
    AAACCTGATG ACTGCAGAGT TTTCATCGCA GAGGCTACTG CCCAGAACAT GGTCTGCTTG
    AAAACAGAAT ATATCGCTGA TTATCTCATG CTGGAATCCC CTCCTTGTGC AGACAATTAC
    CGTGTATCCG AAGCTGCTTT ATTCCATAAT AAGAAAGGTG GGCCTGGATT ACCACAGAAG
    AGGAAAACTC CTGCAGAGAA TGTAGTCAAA CGGCCTCGAG TACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_795953.2
    CDS53..4600
    Translation

    Target ORF information:

    RefSeq Version NM_176979.5
    Organism Mus musculus(house mouse)
    Definition Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176979.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG
    ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT
    CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA
    GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA
    CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT
    AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA
    CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT
    ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT
    GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT
    GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT
    GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC
    TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT
    AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG
    GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA
    GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC
    TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG
    ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG
    CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT
    GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC
    CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC
    AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT
    TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG
    CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA
    CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT
    ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC
    TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG
    GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA
    TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG
    AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC
    ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG
    GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT
    TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG
    GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG
    CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT
    TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT
    GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA
    CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT
    TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG
    GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG
    AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC
    GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT
    AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGTGTGCT
    CAAGAGTATA AGCATCTTCC TGAATCTCTT TATCCACACA CTTACAATCC CAAAATGAGT
    TTGGACATCA ACACAGTGCA AGATGGGCGG CTCTGTAACA GCCGGGCACC CTTGGCAGTT
    TCTGCATCTA AGGATGATGG GCCAGATCAT CTGTCTGTAG AAGGAAATGA GACAAACACT
    ATGGGCACAA ATGACAAAGA GTCACCTCTG TTGAATGGGA GTGGAAGAGA TGACTGTAAA
    GGAGCTCTGA CCCAGGCCTT GGAGATGAGA GAGAACTTCC AGAAGCAGCT GCAAGAGATC
    ATGTCAGCCA CCTGCATTGT GAAGACGCCT GCCCAGAAGA CTTGCATGTC AAGAAGCAGC
    TGTAACAGCG CCTCTTCAAC TCCTGACAGC GCTCGCTCTG TGCGGAGCGG ACGAAGCCGA
    GTCCTGGAGG CACTCAGGCA GTCTCGGCAG GCAGTGCCTG ATGTGAACAC GGAGCCGTCC
    CAGAACGAGC AGATCATTTG GGACGACCCG ACAGCAAGGG AAGAGAGAGC AAGGCTTGCC
    AGCAACTTAC AGTGGCCCAG CGACCCCACA CAGCATTCTG AGCTTCAGGT TGAGATAAAA
    ATGCCGGATG ACTCTCCCTC TCGAAAGCCT GTGTACCATT CAGAAATTGC TGAGCAGGCC
    TCGTGTGTTA CTCAAGCCCC AGGGCACCCA GGCTCTGAAG AACCAGAGCC TCCAGTAGCA
    GAAAGGCCTT TGATCCCCGA GCCGCAAGCC CCCGCTGTAG CTTCCCCCCT GGCTAAGCCG
    CCTGTGGCCC CACAACCTGC AGACAAGATT GAAACACAGG AGGAGACGCA CAGAAAAGTT
    AAAAAACAGT ATGTGTTCCA GATGTCGTCT CTGAATTCTC AAGAGCGGAT TGATTACTGT
    CGCCTGATTA AAGACTTAGG TGGGTCGGTG ATCGAGAAGC AGTGCTCTGA CCCCAGCTGC
    ACACACATGG TTGTGGGCTA CCCACTGCGC AATGAGAAGT ACTTAGCTTC TATGGCAGCT
    GGTAAATGGG TGCTTCATCG CTCCTACCTA GACGCTTGCA AGACAGCTGG GCGCTTTGTG
    CAGGAAGAAG ACTACGAGTG GGGAAGTAGC TCCATACTTG ATGCTCTCCC TGACGTCACT
    GAGCACCAGC AGAAGCTCGC ACTGGCGGCA ATGAGATGGA GGAAAAGAAT CCAGCAAAGT
    CAAGAATCCG GCATTGTTGA GGGAGCATTT AGTGGCTGGA AAGCTATTTT ACGTGTTGAT
    CGGCCCCGAG AAGCTGGGTT CAAACGCCTT CTTCAGGCAG GAGGAGCAAA GGTGTTATCT
    GGTCATCCAG AACCTTTACT TAAAGATGCT ACGCATCTCT TTTGTGACTT CAATAAACTG
    AAACCTGATG ACTGCAGAGT TTTCATCGCA GAGGCTACTG CCCAGAACAT GGTCTGCTTG
    AAAACAGAAT ATATCGCTGA TTATCTCATG CTGGAATCCC CTCCTTGTGC AGACAATTAC
    CGTGTATCCG AAGCTGCTTT ATTCCATAAT AAGAAAGGTG GGCCTGGATT ACCACAGAAG
    AGGAAAACTC CTGCAGAGAA TGTAGTCAAA CGGCCTCGAG TACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu19740
    Clone ID Related Accession (Same CDS sequence) XM_006511707.3 , XM_006511707.4 , NM_176979.5
    Accession Version XM_006511707.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product DNA topoisomerase 2-binding protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511707.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG
    ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT
    CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA
    GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA
    CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT
    AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA
    CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT
    ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT
    GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT
    GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT
    GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC
    TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT
    AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG
    GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA
    GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC
    TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG
    ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG
    CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT
    GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC
    CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC
    AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT
    TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG
    CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA
    CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT
    ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC
    TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG
    GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA
    TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG
    AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC
    ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG
    GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT
    TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG
    GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG
    CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT
    TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT
    GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA
    CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT
    TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG
    GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG
    AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC
    GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT
    AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGTGTGCT
    CAAGAGTATA AGCATCTTCC TGAATCTCTT TATCCACACA CTTACAATCC CAAAATGAGT
    TTGGACATCA ACACAGTGCA AGATGGGCGG CTCTGTAACA GCCGGGCACC CTTGGCAGTT
    TCTGCATCTA AGGATGATGG GCCAGATCAT CTGTCTGTAG AAGGAAATGA GACAAACACT
    ATGGGCACAA ATGACAAAGA GTCACCTCTG TTGAATGGGA GTGGAAGAGA TGACTGTAAA
    GGAGCTCTGA CCCAGGCCTT GGAGATGAGA GAGAACTTCC AGAAGCAGCT GCAAGAGATC
    ATGTCAGCCA CCTGCATTGT GAAGACGCCT GCCCAGAAGA CTTGCATGTC AAGAAGCAGC
    TGTAACAGCG CCTCTTCAAC TCCTGACAGC GCTCGCTCTG TGCGGAGCGG ACGAAGCCGA
    GTCCTGGAGG CACTCAGGCA GTCTCGGCAG GCAGTGCCTG ATGTGAACAC GGAGCCGTCC
    CAGAACGAGC AGATCATTTG GGACGACCCG ACAGCAAGGG AAGAGAGAGC AAGGCTTGCC
    AGCAACTTAC AGTGGCCCAG CGACCCCACA CAGCATTCTG AGCTTCAGGT TGAGATAAAA
    ATGCCGGATG ACTCTCCCTC TCGAAAGCCT GTGTACCATT CAGAAATTGC TGAGCAGGCC
    TCGTGTGTTA CTCAAGCCCC AGGGCACCCA GGCTCTGAAG AACCAGAGCC TCCAGTAGCA
    GAAAGGCCTT TGATCCCCGA GCCGCAAGCC CCCGCTGTAG CTTCCCCCCT GGCTAAGCCG
    CCTGTGGCCC CACAACCTGC AGACAAGATT GAAACACAGG AGGAGACGCA CAGAAAAGTT
    AAAAAACAGT ATGTGTTCCA GATGTCGTCT CTGAATTCTC AAGAGCGGAT TGATTACTGT
    CGCCTGATTA AAGACTTAGG TGGGTCGGTG ATCGAGAAGC AGTGCTCTGA CCCCAGCTGC
    ACACACATGG TTGTGGGCTA CCCACTGCGC AATGAGAAGT ACTTAGCTTC TATGGCAGCT
    GGTAAATGGG TGCTTCATCG CTCCTACCTA GACGCTTGCA AGACAGCTGG GCGCTTTGTG
    CAGGAAGAAG ACTACGAGTG GGGAAGTAGC TCCATACTTG ATGCTCTCCC TGACGTCACT
    GAGCACCAGC AGAAGCTCGC ACTGGCGGCA ATGAGATGGA GGAAAAGAAT CCAGCAAAGT
    CAAGAATCCG GCATTGTTGA GGGAGCATTT AGTGGCTGGA AAGCTATTTT ACGTGTTGAT
    CGGCCCCGAG AAGCTGGGTT CAAACGCCTT CTTCAGGCAG GAGGAGCAAA GGTGTTATCT
    GGTCATCCAG AACCTTTACT TAAAGATGCT ACGCATCTCT TTTGTGACTT CAATAAACTG
    AAACCTGATG ACTGCAGAGT TTTCATCGCA GAGGCTACTG CCCAGAACAT GGTCTGCTTG
    AAAACAGAAT ATATCGCTGA TTATCTCATG CTGGAATCCC CTCCTTGTGC AGACAATTAC
    CGTGTATCCG AAGCTGCTTT ATTCCATAAT AAGAAAGGTG GGCCTGGATT ACCACAGAAG
    AGGAAAACTC CTGCAGAGAA TGTAGTCAAA CGGCCTCGAG TACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006511770.1
    CDS142..4689
    Translation

    Target ORF information:

    RefSeq Version XM_006511707.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006511707.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG
    ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT
    CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA
    GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA
    CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT
    AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA
    CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT
    ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT
    GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT
    GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT
    GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC
    TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT
    AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG
    GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA
    GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC
    TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG
    ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG
    CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT
    GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC
    CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC
    AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT
    TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG
    CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA
    CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT
    ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC
    TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG
    GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA
    TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG
    AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC
    ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG
    GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT
    TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG
    GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG
    CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT
    TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT
    GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA
    CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT
    TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG
    GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG
    AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC
    GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT
    AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGTGTGCT
    CAAGAGTATA AGCATCTTCC TGAATCTCTT TATCCACACA CTTACAATCC CAAAATGAGT
    TTGGACATCA ACACAGTGCA AGATGGGCGG CTCTGTAACA GCCGGGCACC CTTGGCAGTT
    TCTGCATCTA AGGATGATGG GCCAGATCAT CTGTCTGTAG AAGGAAATGA GACAAACACT
    ATGGGCACAA ATGACAAAGA GTCACCTCTG TTGAATGGGA GTGGAAGAGA TGACTGTAAA
    GGAGCTCTGA CCCAGGCCTT GGAGATGAGA GAGAACTTCC AGAAGCAGCT GCAAGAGATC
    ATGTCAGCCA CCTGCATTGT GAAGACGCCT GCCCAGAAGA CTTGCATGTC AAGAAGCAGC
    TGTAACAGCG CCTCTTCAAC TCCTGACAGC GCTCGCTCTG TGCGGAGCGG ACGAAGCCGA
    GTCCTGGAGG CACTCAGGCA GTCTCGGCAG GCAGTGCCTG ATGTGAACAC GGAGCCGTCC
    CAGAACGAGC AGATCATTTG GGACGACCCG ACAGCAAGGG AAGAGAGAGC AAGGCTTGCC
    AGCAACTTAC AGTGGCCCAG CGACCCCACA CAGCATTCTG AGCTTCAGGT TGAGATAAAA
    ATGCCGGATG ACTCTCCCTC TCGAAAGCCT GTGTACCATT CAGAAATTGC TGAGCAGGCC
    TCGTGTGTTA CTCAAGCCCC AGGGCACCCA GGCTCTGAAG AACCAGAGCC TCCAGTAGCA
    GAAAGGCCTT TGATCCCCGA GCCGCAAGCC CCCGCTGTAG CTTCCCCCCT GGCTAAGCCG
    CCTGTGGCCC CACAACCTGC AGACAAGATT GAAACACAGG AGGAGACGCA CAGAAAAGTT
    AAAAAACAGT ATGTGTTCCA GATGTCGTCT CTGAATTCTC AAGAGCGGAT TGATTACTGT
    CGCCTGATTA AAGACTTAGG TGGGTCGGTG ATCGAGAAGC AGTGCTCTGA CCCCAGCTGC
    ACACACATGG TTGTGGGCTA CCCACTGCGC AATGAGAAGT ACTTAGCTTC TATGGCAGCT
    GGTAAATGGG TGCTTCATCG CTCCTACCTA GACGCTTGCA AGACAGCTGG GCGCTTTGTG
    CAGGAAGAAG ACTACGAGTG GGGAAGTAGC TCCATACTTG ATGCTCTCCC TGACGTCACT
    GAGCACCAGC AGAAGCTCGC ACTGGCGGCA ATGAGATGGA GGAAAAGAAT CCAGCAAAGT
    CAAGAATCCG GCATTGTTGA GGGAGCATTT AGTGGCTGGA AAGCTATTTT ACGTGTTGAT
    CGGCCCCGAG AAGCTGGGTT CAAACGCCTT CTTCAGGCAG GAGGAGCAAA GGTGTTATCT
    GGTCATCCAG AACCTTTACT TAAAGATGCT ACGCATCTCT TTTGTGACTT CAATAAACTG
    AAACCTGATG ACTGCAGAGT TTTCATCGCA GAGGCTACTG CCCAGAACAT GGTCTGCTTG
    AAAACAGAAT ATATCGCTGA TTATCTCATG CTGGAATCCC CTCCTTGTGC AGACAATTAC
    CGTGTATCCG AAGCTGCTTT ATTCCATAAT AAGAAAGGTG GGCCTGGATT ACCACAGAAG
    AGGAAAACTC CTGCAGAGAA TGTAGTCAAA CGGCCTCGAG TACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu73844
    Clone ID Related Accession (Same CDS sequence) XM_006511706.4 , XM_006511706.3
    Accession Version XM_006511706.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG
    ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT
    CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA
    GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA
    CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT
    AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA
    CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT
    ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT
    GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT
    GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT
    GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC
    TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT
    AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG
    GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA
    GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC
    TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG
    ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG
    CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT
    GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC
    CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC
    AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT
    TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG
    CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA
    CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT
    ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC
    TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG
    GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA
    TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG
    AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC
    ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG
    GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT
    TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG
    GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG
    CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT
    TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT
    GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA
    CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT
    TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG
    GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG
    AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC
    GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT
    AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGCCAGAT
    CATCTGTCTG TAGAAGGAAA TGAGACAAAC ACTATGGGCA CAAATGACAA AGAGTCACCT
    CTGTTGAATG GGAGTGGAAG AGATGACTGT AAAGGAGCTC TGACCCAGGC CTTGGAGATG
    AGAGAGAACT TCCAGAAGCA GCTGCAAGAG ATCATGTCAG CCACCTGCAT TGTGAAGACG
    CCTGCCCAGA AGACTTGCAT GTCAAGAAGC AGCTGTAACA GCGCCTCTTC AACTCCTGAC
    AGCGCTCGCT CTGTGCGGAG CGGACGAAGC CGAGTCCTGG AGGCACTCAG GCAGTCTCGG
    CAGGCAGTGC CTGATGTGAA CACGGAGCCG TCCCAGAACG AGCAGATCAT TTGGGACGAC
    CCGACAGCAA GGGAAGAGAG AGCAAGGCTT GCCAGCAACT TACAGTGGCC CAGCGACCCC
    ACACAGCATT CTGAGCTTCA GGTTGAGATA AAAATGCCGG ATGACTCTCC CTCTCGAAAG
    CCTGTGTACC ATTCAGAAAT TGCTGAGCAG GCCTCGTGTG TTACTCAAGC CCCAGGGCAC
    CCAGGCTCTG AAGAACCAGA GCCTCCAGTA GCAGAAAGGC CTTTGATCCC CGAGCCGCAA
    GCCCCCGCTG TAGCTTCCCC CCTGGCTAAG CCGCCTGTGG CCCCACAACC TGCAGACAAG
    ATTGAAACAC AGGAGGAGAC GCACAGAAAA GTTAAAAAAC AGTATGTGTT CCAGATGTCG
    TCTCTGAATT CTCAAGAGCG GATTGATTAC TGTCGCCTGA TTAAAGACTT AGGTGGGTCG
    GTGATCGAGA AGCAGTGCTC TGACCCCAGC TGCACACACA TGGTTGTGGG CTACCCACTG
    CGCAATGAGA AGTACTTAGC TTCTATGGCA GCTGGTAAAT GGGTGCTTCA TCGCTCCTAC
    CTAGACGCTT GCAAGACAGC TGGGCGCTTT GTGCAGGAAG AAGACTACGA GTGGGGAAGT
    AGCTCCATAC TTGATGCTCT CCCTGACGTC ACTGAGCACC AGCAGAAGCT CGCACTGGCG
    GCAATGAGAT GGAGGAAAAG AATCCAGCAA AGTCAAGAAT CCGGCATTGT TGAGGGAGCA
    TTTAGTGGCT GGAAAGCTAT TTTACGTGTT GATCGGCCCC GAGAAGCTGG GTTCAAACGC
    CTTCTTCAGG CAGGAGGAGC AAAGGTGTTA TCTGGTCATC CAGAACCTTT ACTTAAAGAT
    GCTACGCATC TCTTTTGTGA CTTCAATAAA CTGAAACCTG ATGACTGCAG AGTTTTCATC
    GCAGAGGCTA CTGCCCAGAA CATGGTCTGC TTGAAAACAG AATATATCGC TGATTATCTC
    ATGCTGGAAT CCCCTCCTTG TGCAGACAAT TACCGTGTAT CCGAAGCTGC TTTATTCCAT
    AATAAGAAAG GTGGGCCTGG ATTACCACAG AAGAGGAAAA CTCCTGCAGA GAATGTAGTC
    AAACGGCCTC GAGTACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006511769.2
    CDS212..4612
    Translation

    Target ORF information:

    RefSeq Version XM_006511706.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006511706.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCCAGAA ATGACCAAGA GCCGTTTCTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGCTTTT TTAAAGCTCT TGAGTCAATA AAGGAATTAC AATCAGAAGA TTATCTTCAG
    ATTATTACAG ATGAGGAAGC ATTGAAGATA AGAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGT TTTTGATCAC CTTAAACAGC TTGGCTGCAG GATTGTTGGT
    CCTCAAGTGG TCACATTTTG TATGCGCCAC CAGCAATGTG TCCCAAGAGC TGAGCATCCA
    GTCTACAACA TGATAATGTC TGACGTAACT GTATCCTGTA CAAGCCTGGA TAAAGACAAA
    CGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAGTACTT AGTTGCTGCT
    AATCTGAAGA AGCCTATTTT GCTACCTTCT TGGATAAAAA CCCTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAGTA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTT
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGAATAC ACAGAAAGAC AGTCCAGCAA
    CTCACGGCTA AGCACGGAGG CCAGTACATG GGACAGTTGA AGATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TACGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT TCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAATCT
    ATATACAAGG CTGAAACCAG AGTAGAAGCA AAGATGGTGC CTGATACTTC AACACCCACT
    GCTCAGAGCA ATGCTGAAAG TCACACGCTC GCAGATGTCA GCCATATATC CAACATAAAT
    GGCAGCTGTG TAAATGAAAC AATGTTTGGT TCAACTACTA GCAAACTTGA ATGTTCCCTT
    GAAAACCTAG AAAATCTGGA CATCAGTATG TTTCAAGCTC CTGAAGACTT ATTAGATGGC
    TGTCGGATAT ATCTTTGTGG TTTTAGTGGC AGAAAGCTGG ATAAGCTGAG AAGGCTTATT
    AACAGTGGAG GTGGAGTTCG ATTTAATCAA CTAAATGAAG ATGTAACTCA TGTAATTGTG
    GGAGATTATG ATGACGACGT GAGGCAGTTC TGGAGCAAAT CATCTCACAG GCCTCACGTA
    GTGGGAGCAA AGTGGTTGCT GGAGTGTTTT ACTAAAGGAT ATATACTTCC TGAAGAGTCC
    TATATCCACA CTAACTACCA GCCAGCTGGA ATTGCAGTTT CCGATCAGCC TGGAAACCAG
    ACAGCTGTCT TAGACAAGAG CGGCTCATTC TCCAAGAGCG CCCTTGTTCC TGCTGAGAGG
    CTTCAGCAGG CCGACGAGGA TCTGCTTGCT CAGTATGGAA ATGACGACTC CACGATGGTT
    GAAGCTAAGC TATCTGAAGC TTTAGAGCCT GAAGTCGGGC CTTGCCCTGG TTCTGCTCAC
    CGTGAGCCCT GTGATGATTC CACTCACATT TCTGTGCAAG AGGAAAACAA GTCGTCTGTC
    AGTCATTGCA TCCTTGATGA CTCTACAGTT CGTGAAGAAG GCTTATTTAG CCAGAAGAGT
    TTCCTTGTTT TGGGTTTTAG TGTTGAAAAT AAATGTAATA TTGTAGACAT CATAAGAGAG
    CATGCTGGCA AGATTGTGTC CCTTCCCAGC AGAATTGTTG CTGACTATGC TGTGGTTCCA
    CTGCTGGGAT GTGAGGTAGA CGTGACGGTG GGAGAAGTTG TTACAAACAC GTGGCTGGTT
    ACGTGTATAG ACAACCAGAC ATTAGTTGAT CCCAAGTCGA ATCCCCTCTT CACACCAGTC
    TCAGTAATGT CGGGAGTGAC TCCTTTAGAG GACTGTGTGA TTTCCTTCAG TCAGTGTGTG
    GGAGCAGAAA GAGATTCCTT GGTATTCTTA GCAAATCACC TTGGAGCAAG TGTTCAAGAA
    TTCTTTGTTC GCAAAGCCAA TGCAAAGAAA GGCATGCTCG CCAGCACACA TCTTATAGTG
    AAGGAACCGA CTGGTTCCAA ATACGAAGCT GCAAAGAAAT GGAGTTTGCC GGCAGTTAAC
    ATTTCATGGC TCTTAGAAAC TGCGAGAATC GGGAAGAGAG CAGATGAAAA CCATTTTCTG
    GTTGACAACG CACCTAAACA AGAACAAGTT TTAGAAACTA AGATACCAAA TGGAGTGAGT
    TCAAATCCAG ATTTACCAGC ACATCCTGAC GCACACTTGG AAATTCACAG AAAAAAGGCG
    GTTACACCTT TGGACATGAA TCGCTTTCAG AGTAGAGCCT TCCGTGCTGT CATTTCACAG
    CAGAGGGGGC AGGACCCCAC CTTTCCACCA GTACGGCAGC CCCTTACTAA GGAGCCCTCT
    TTACACCTGG ACACACCATC AAAGTTTCTG TCCAAGGACA AGCTTTTCAA GCCATCCTTT
    GATGTGACGG ATGCACTTGC AGCCCTGGAA ACCCCAAATG CTGCCAGCCA GAAGCGGAAA
    CTAAGCTCAC CACTCTCTGA AGTCATCGTC AGAAACCTGA CAGTTGCTTT GGCAAATAGT
    TCCCGAAACA CTGACAGTCA TTCTGCCAGC CCTCAGCTGA AGGGTGCCCA TTTGGAGGAG
    GAAGAGACTC GGAAACCACT TGACAGCGTT GTGGTGTGTG TGAGTAAGAA GCTCAGTAAG
    AAGCAGAGTG AGCTCAATGG GGTCGCAGCA TCACTGGGAG CAGAGTACAG GTGGAGTTTC
    GATGAGACTG TTACTCATTT CATTTACCAG GGTCGGGCAA ATGACAGTAA TCGAGAATAT
    AAATCTGCAA AGGAAAGAGG GGTGCACATC GTGTCCGAGC ATTGGCTATT AGAGCCAGAT
    CATCTGTCTG TAGAAGGAAA TGAGACAAAC ACTATGGGCA CAAATGACAA AGAGTCACCT
    CTGTTGAATG GGAGTGGAAG AGATGACTGT AAAGGAGCTC TGACCCAGGC CTTGGAGATG
    AGAGAGAACT TCCAGAAGCA GCTGCAAGAG ATCATGTCAG CCACCTGCAT TGTGAAGACG
    CCTGCCCAGA AGACTTGCAT GTCAAGAAGC AGCTGTAACA GCGCCTCTTC AACTCCTGAC
    AGCGCTCGCT CTGTGCGGAG CGGACGAAGC CGAGTCCTGG AGGCACTCAG GCAGTCTCGG
    CAGGCAGTGC CTGATGTGAA CACGGAGCCG TCCCAGAACG AGCAGATCAT TTGGGACGAC
    CCGACAGCAA GGGAAGAGAG AGCAAGGCTT GCCAGCAACT TACAGTGGCC CAGCGACCCC
    ACACAGCATT CTGAGCTTCA GGTTGAGATA AAAATGCCGG ATGACTCTCC CTCTCGAAAG
    CCTGTGTACC ATTCAGAAAT TGCTGAGCAG GCCTCGTGTG TTACTCAAGC CCCAGGGCAC
    CCAGGCTCTG AAGAACCAGA GCCTCCAGTA GCAGAAAGGC CTTTGATCCC CGAGCCGCAA
    GCCCCCGCTG TAGCTTCCCC CCTGGCTAAG CCGCCTGTGG CCCCACAACC TGCAGACAAG
    ATTGAAACAC AGGAGGAGAC GCACAGAAAA GTTAAAAAAC AGTATGTGTT CCAGATGTCG
    TCTCTGAATT CTCAAGAGCG GATTGATTAC TGTCGCCTGA TTAAAGACTT AGGTGGGTCG
    GTGATCGAGA AGCAGTGCTC TGACCCCAGC TGCACACACA TGGTTGTGGG CTACCCACTG
    CGCAATGAGA AGTACTTAGC TTCTATGGCA GCTGGTAAAT GGGTGCTTCA TCGCTCCTAC
    CTAGACGCTT GCAAGACAGC TGGGCGCTTT GTGCAGGAAG AAGACTACGA GTGGGGAAGT
    AGCTCCATAC TTGATGCTCT CCCTGACGTC ACTGAGCACC AGCAGAAGCT CGCACTGGCG
    GCAATGAGAT GGAGGAAAAG AATCCAGCAA AGTCAAGAAT CCGGCATTGT TGAGGGAGCA
    TTTAGTGGCT GGAAAGCTAT TTTACGTGTT GATCGGCCCC GAGAAGCTGG GTTCAAACGC
    CTTCTTCAGG CAGGAGGAGC AAAGGTGTTA TCTGGTCATC CAGAACCTTT ACTTAAAGAT
    GCTACGCATC TCTTTTGTGA CTTCAATAAA CTGAAACCTG ATGACTGCAG AGTTTTCATC
    GCAGAGGCTA CTGCCCAGAA CATGGTCTGC TTGAAAACAG AATATATCGC TGATTATCTC
    ATGCTGGAAT CCCCTCCTTG TGCAGACAAT TACCGTGTAT CCGAAGCTGC TTTATTCCAT
    AATAAGAAAG GTGGGCCTGG ATTACCACAG AAGAGGAAAA CTCCTGCAGA GAATGTAGTC
    AAACGGCCTC GAGTACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.