Arhgef10 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006508771.3	XP_006508834.1	rho guanine nucleotide exchange factor 10 isoform X7
XM_006508773.4	XP_006508836.1	rho guanine nucleotide exchange factor 10 isoform X10
XM_006508768.3	XP_006508831.1	rho guanine nucleotide exchange factor 10 isoform X1
XM_017312669.2	XP_017168158.1	rho guanine nucleotide exchange factor 10 isoform X3
XM_017312672.1	XP_017168161.1	rho guanine nucleotide exchange factor 10 isoform X6
XM_006508773.3	XP_006508836.1	rho guanine nucleotide exchange factor 10 isoform X9
XM_017312669.1	XP_017168158.1	rho guanine nucleotide exchange factor 10 isoform X3
XM_017312670.2	XP_017168159.1	rho guanine nucleotide exchange factor 10 isoform X4
XM_006508770.3	XP_006508833.1	rho guanine nucleotide exchange factor 10 isoform X7
XM_006508771.4	XP_006508834.1	rho guanine nucleotide exchange factor 10 isoform X7
XM_017312668.1	XP_017168157.1	rho guanine nucleotide exchange factor 10 isoform X2
XM_030243425.1	XP_030099285.1	rho guanine nucleotide exchange factor 10 isoform X7
XM_017312670.1	XP_017168159.1	rho guanine nucleotide exchange factor 10 isoform X4
NM_172751.3	NP_766339.2	rho guanine nucleotide exchange factor 10 isoform a
NM_001037736.2	NP_001032825.1	rho guanine nucleotide exchange factor 10 isoform b
XM_017312671.1	XP_017168160.1	rho guanine nucleotide exchange factor 10 isoform X5
XM_017312673.1	XP_017168162.1	rho guanine nucleotide exchange factor 10 isoform X7
XM_006508772.3	XP_006508835.1	rho guanine nucleotide exchange factor 10 isoform X8
XM_030243426.1	XP_030099286.1	rho guanine nucleotide exchange factor 10 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu40386	XM_006508773.4 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu40384	XM_006508771.4 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu40384	XM_030243425.1 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu73730	XM_030243426.1 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu82406	XM_017312668.1 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu82407	XM_017312669.2 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu82408	XM_017312670.2 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu82409	XM_017312671.1 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu82410	XM_017312672.1 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu12832	NM_001037736.2 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu03998	NM_172751.3 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$790.30 $1129.00
OMu40382	XM_006508768.3 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu40384	XM_017312673.1 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu40384	XM_006508770.3 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu40385	XM_006508772.3 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu40386	XM_006508773.3	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu82407	XM_017312669.1	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu40384	XM_006508771.3	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu82408	XM_017312670.1	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

Arhgef10 ( XM_006508773.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508836.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu40386
Clone ID Related Accession (Same CDS sequence)	XM_006508773.4 , XM_006508773.3
Accession Version	XM_006508773.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508773.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAATG ACGTGCTCAT GTGTGCCACC GCCAGCTCAC GGAACTCACA TGAAAGCCAT 
GCCGTGATGA GCCAGAGGTA CCTGCTCAAG TGGAGTGTCC CTCTAGGTCA CGTGGATGTG 
ATTCAGTACA ATGGAGGCTC TGGCGCCGGG GAGCACTGTC GGCACCACGC TGCACACTCA 
CCTGAGAGCC TGGCCGTGGT GGCCAACGCG AAGCCACACA AAGTTTACAT GGGGCCAGGG 
CAGCTGTACC AGGATCTGCA GAACCTACTG CACGACCTGA ACGTTGTTGG CCAGATCTCG 
CAGCTGATAG GAAACCTCCG AGGGAGCTAT CAGAACTTAA ACCAGTCTGT AGCCCATGAT 
TGGACGTCGG GCTTACAGCG GCTGATACTG AGGAAAGAAG ATGCGATCAG AGCTGCCGAC 
CGCTGCCGGA TCCAGTTACA GCTCCCGGGA AAGCAGGACA AGTCCGGGCG GCCCACGTTC 
TTCACTGCTG TGTTCAATAC GCTTACACCA GCCATCAAGG AGTCCTGGGT CAGCAGCCTG 
CAGATGGCCA AGCTGGCCCT TGAGGAGGAG AACCATATGG GATGGTTCTG TGTGGATGAC 
GATGGCAACC TAGCCAAGAA GGAGACACAC CCTCTCCTGG TGGGACACAT GCCTGTGATG 
GTGGCCAAGC AGCCAGAGTT CAAGATTGAA TGTGCAGCTT ATAACCCTGA ACCTTACCTG 
AGCAATGAAA GTCAACCAGC CTCACCCTCC ACAGCCCGTG GCTTTCTGTG GATTGGAAGC 
TGTAGCAACC AGATGGGTCA GGTCGCCATT GTCTCCTTCC AAGGTTCCAA CCCCAAAGTC 
ATCGAGTGCT TCAATGTGGA ATCACGCATC CTGTGTATGG TATACATTCC CGCAGAGGAG 
TCAAAGCCCC AAGAAACCAC CGAAACCAAG GATCCAGAAG CCACAGCCTC GAGAGCACCC 
CATGTGCCCA CCATCTGCCT GGGCACAGAA GAGGGAAGCA TTTCCATTTA CAAAAGCAGT 
CAGGGCTGCA AGAAGGTGAG GCTTCAGCAC TTCTATGCAC CGGACAAGTC CACGGTCATG 
AGCTTGGCCT GCTCCCCACA AGGCCTGTAT GCAGGCCTGG TCAATGGGTC CGTAGCCAGC 
TACACCAAGG CTCCAGCACC GAGCTCTCCT GGCAGCCCGT GGGCAGAGTT CTGTCTCATC 
TGCGTTTTCC TGCCATGCTT GCCCTCGGGA GCATATGCCA TCTCGGGTAC AGGCTGTGGT 
GCAGTGAAGG GCTATGGATC CTGGAACTCG GAACCCCAGC AAGTGATCAA GTTGGGTGTC 
CTGCCAGTCA GAAGCCTTCT CCTGGTGGAG GGTGCACTGT GGGCAGCCTC GGGAGGGCAG 
GTGTTCATGG CCAGCGTGGA GACTCACACT ATAGAGAATC AGCTGGAGGC CCACCAAGAT 
GAAGGGATGG TCATCTCCCA CATGGCTGTG GCTGGCGTTG GCATCTGGAT TGCCTTCACC 
TCGGGGTCCA CCCTCCGCCT CTTCCACACA GAGACCTTGA AGCACCTGCA GGATGTCAAC 
ATTGATGCCC CTGTGCATAG CATGCTGCCA GGGCACCAGC GTCTATCTGT GACCAGCCTG 
CTCGTCTGCC ACGGCTTGCT CATGGTCGGT ACCAGCCTGG GTGTCGTGGT GGCCCTGCCA 
GTCCCTCGGC TTCAGGGTAT CCCCAAAGTG ACAGGGCGAG GCATGGTCTC CTACCATGCG 
CATAACGGGC CCGTTAAGTT CATCGTCTCA GCCACGGCGT TTCAAAACAA GGACAGAGCC 
AGGGACAGCC CACGTTCCGG TTCTGAGCTT CAGGATGAAG ACCCCAAGGA CTTGCTCTGC 
AGCGAGGAAG GTCCTTCCTG CCCGGGCCAG CCTGACACCT ACACATCCGT TTGGTTGGGA 
GACTCCCTAG GATTGCCAAC TCAAAAGAAT GATCTGTCTT CGTCCTCGGG GTCACTGAAC 
CTGTCCCATG GCTCCAGTTC CCTAGAGCAC AGATCTGTGG ACAGCAGTCT GTGTGACCTG 
CTGAGGGACC CCAGTGCCTC CCCAAGGAGC AGACCACAGG GCTCTAGGCG AGCAAGAGCC 
AGTTCAGCGC TGGTGGTATG TGGGGGCCAG GGCCACCGGC GGGTGCACAG GAAGGCCAGG 
CAGCCTTCTC AGGAGGACCT GGTTTCTTCA GTGATGGTGT GGCAGATCCC CCTGCTGGGC 
ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508836.1
CDS	462..2687
Translation	MLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPAPSSPGSPWAEFCLICVFLPCLPSGAYAISGTGCGAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_006508773.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508773.3

ORF Insert Sequence:

ATGCTGAATG ACGTGCTCAT GTGTGCCACC GCCAGCTCAC GGAACTCACA TGAAAGCCAT 
GCCGTGATGA GCCAGAGGTA CCTGCTCAAG TGGAGTGTCC CTCTAGGTCA CGTGGATGTG 
ATTCAGTACA ATGGAGGCTC TGGCGCCGGG GAGCACTGTC GGCACCACGC TGCACACTCA 
CCTGAGAGCC TGGCCGTGGT GGCCAACGCG AAGCCACACA AAGTTTACAT GGGGCCAGGG 
CAGCTGTACC AGGATCTGCA GAACCTACTG CACGACCTGA ACGTTGTTGG CCAGATCTCG 
CAGCTGATAG GAAACCTCCG AGGGAGCTAT CAGAACTTAA ACCAGTCTGT AGCCCATGAT 
TGGACGTCGG GCTTACAGCG GCTGATACTG AGGAAAGAAG ATGCGATCAG AGCTGCCGAC 
CGCTGCCGGA TCCAGTTACA GCTCCCGGGA AAGCAGGACA AGTCCGGGCG GCCCACGTTC 
TTCACTGCTG TGTTCAATAC GCTTACACCA GCCATCAAGG AGTCCTGGGT CAGCAGCCTG 
CAGATGGCCA AGCTGGCCCT TGAGGAGGAG AACCATATGG GATGGTTCTG TGTGGATGAC 
GATGGCAACC TAGCCAAGAA GGAGACACAC CCTCTCCTGG TGGGACACAT GCCTGTGATG 
GTGGCCAAGC AGCCAGAGTT CAAGATTGAA TGTGCAGCTT ATAACCCTGA ACCTTACCTG 
AGCAATGAAA GTCAACCAGC CTCACCCTCC ACAGCCCGTG GCTTTCTGTG GATTGGAAGC 
TGTAGCAACC AGATGGGTCA GGTCGCCATT GTCTCCTTCC AAGGTTCCAA CCCCAAAGTC 
ATCGAGTGCT TCAATGTGGA ATCACGCATC CTGTGTATGG TATACATTCC CGCAGAGGAG 
TCAAAGCCCC AAGAAACCAC CGAAACCAAG GATCCAGAAG CCACAGCCTC GAGAGCACCC 
CATGTGCCCA CCATCTGCCT GGGCACAGAA GAGGGAAGCA TTTCCATTTA CAAAAGCAGT 
CAGGGCTGCA AGAAGGTGAG GCTTCAGCAC TTCTATGCAC CGGACAAGTC CACGGTCATG 
AGCTTGGCCT GCTCCCCACA AGGCCTGTAT GCAGGCCTGG TCAATGGGTC CGTAGCCAGC 
TACACCAAGG CTCCAGCACC GAGCTCTCCT GGCAGCCCGT GGGCAGAGTT CTGTCTCATC 
TGCGTTTTCC TGCCATGCTT GCCCTCGGGA GCATATGCCA TCTCGGGTAC AGGCTGTGGT 
GCAGTGAAGG GCTATGGATC CTGGAACTCG GAACCCCAGC AAGTGATCAA GTTGGGTGTC 
CTGCCAGTCA GAAGCCTTCT CCTGGTGGAG GGTGCACTGT GGGCAGCCTC GGGAGGGCAG 
GTGTTCATGG CCAGCGTGGA GACTCACACT ATAGAGAATC AGCTGGAGGC CCACCAAGAT 
GAAGGGATGG TCATCTCCCA CATGGCTGTG GCTGGCGTTG GCATCTGGAT TGCCTTCACC 
TCGGGGTCCA CCCTCCGCCT CTTCCACACA GAGACCTTGA AGCACCTGCA GGATGTCAAC 
ATTGATGCCC CTGTGCATAG CATGCTGCCA GGGCACCAGC GTCTATCTGT GACCAGCCTG 
CTCGTCTGCC ACGGCTTGCT CATGGTCGGT ACCAGCCTGG GTGTCGTGGT GGCCCTGCCA 
GTCCCTCGGC TTCAGGGTAT CCCCAAAGTG ACAGGGCGAG GCATGGTCTC CTACCATGCG 
CATAACGGGC CCGTTAAGTT CATCGTCTCA GCCACGGCGT TTCAAAACAA GGACAGAGCC 
AGGGACAGCC CACGTTCCGG TTCTGAGCTT CAGGATGAAG ACCCCAAGGA CTTGCTCTGC 
AGCGAGGAAG GTCCTTCCTG CCCGGGCCAG CCTGACACCT ACACATCCGT TTGGTTGGGA 
GACTCCCTAG GATTGCCAAC TCAAAAGAAT GATCTGTCTT CGTCCTCGGG GTCACTGAAC 
CTGTCCCATG GCTCCAGTTC CCTAGAGCAC AGATCTGTGG ACAGCAGTCT GTGTGACCTG 
CTGAGGGACC CCAGTGCCTC CCCAAGGAGC AGACCACAGG GCTCTAGGCG AGCAAGAGCC 
AGTTCAGCGC TGGTGGTATG TGGGGGCCAG GGCCACCGGC GGGTGCACAG GAAGGCCAGG 
CAGCCTTCTC AGGAGGACCT GGTTTCTTCA GTGATGGTGT GGCAGATCCC CCTGCTGGGC 
ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_006508773.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508836.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu40386
Clone ID Related Accession (Same CDS sequence)	XM_006508773.4 , XM_006508773.3
Accession Version	XM_006508773.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508773.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAATG ACGTGCTCAT GTGTGCCACC GCCAGCTCAC GGAACTCACA TGAAAGCCAT 
GCCGTGATGA GCCAGAGGTA CCTGCTCAAG TGGAGTGTCC CTCTAGGTCA CGTGGATGTG 
ATTCAGTACA ATGGAGGCTC TGGCGCCGGG GAGCACTGTC GGCACCACGC TGCACACTCA 
CCTGAGAGCC TGGCCGTGGT GGCCAACGCG AAGCCACACA AAGTTTACAT GGGGCCAGGG 
CAGCTGTACC AGGATCTGCA GAACCTACTG CACGACCTGA ACGTTGTTGG CCAGATCTCG 
CAGCTGATAG GAAACCTCCG AGGGAGCTAT CAGAACTTAA ACCAGTCTGT AGCCCATGAT 
TGGACGTCGG GCTTACAGCG GCTGATACTG AGGAAAGAAG ATGCGATCAG AGCTGCCGAC 
CGCTGCCGGA TCCAGTTACA GCTCCCGGGA AAGCAGGACA AGTCCGGGCG GCCCACGTTC 
TTCACTGCTG TGTTCAATAC GCTTACACCA GCCATCAAGG AGTCCTGGGT CAGCAGCCTG 
CAGATGGCCA AGCTGGCCCT TGAGGAGGAG AACCATATGG GATGGTTCTG TGTGGATGAC 
GATGGCAACC TAGCCAAGAA GGAGACACAC CCTCTCCTGG TGGGACACAT GCCTGTGATG 
GTGGCCAAGC AGCCAGAGTT CAAGATTGAA TGTGCAGCTT ATAACCCTGA ACCTTACCTG 
AGCAATGAAA GTCAACCAGC CTCACCCTCC ACAGCCCGTG GCTTTCTGTG GATTGGAAGC 
TGTAGCAACC AGATGGGTCA GGTCGCCATT GTCTCCTTCC AAGGTTCCAA CCCCAAAGTC 
ATCGAGTGCT TCAATGTGGA ATCACGCATC CTGTGTATGG TATACATTCC CGCAGAGGAG 
TCAAAGCCCC AAGAAACCAC CGAAACCAAG GATCCAGAAG CCACAGCCTC GAGAGCACCC 
CATGTGCCCA CCATCTGCCT GGGCACAGAA GAGGGAAGCA TTTCCATTTA CAAAAGCAGT 
CAGGGCTGCA AGAAGGTGAG GCTTCAGCAC TTCTATGCAC CGGACAAGTC CACGGTCATG 
AGCTTGGCCT GCTCCCCACA AGGCCTGTAT GCAGGCCTGG TCAATGGGTC CGTAGCCAGC 
TACACCAAGG CTCCAGCACC GAGCTCTCCT GGCAGCCCGT GGGCAGAGTT CTGTCTCATC 
TGCGTTTTCC TGCCATGCTT GCCCTCGGGA GCATATGCCA TCTCGGGTAC AGGCTGTGGT 
GCAGTGAAGG GCTATGGATC CTGGAACTCG GAACCCCAGC AAGTGATCAA GTTGGGTGTC 
CTGCCAGTCA GAAGCCTTCT CCTGGTGGAG GGTGCACTGT GGGCAGCCTC GGGAGGGCAG 
GTGTTCATGG CCAGCGTGGA GACTCACACT ATAGAGAATC AGCTGGAGGC CCACCAAGAT 
GAAGGGATGG TCATCTCCCA CATGGCTGTG GCTGGCGTTG GCATCTGGAT TGCCTTCACC 
TCGGGGTCCA CCCTCCGCCT CTTCCACACA GAGACCTTGA AGCACCTGCA GGATGTCAAC 
ATTGATGCCC CTGTGCATAG CATGCTGCCA GGGCACCAGC GTCTATCTGT GACCAGCCTG 
CTCGTCTGCC ACGGCTTGCT CATGGTCGGT ACCAGCCTGG GTGTCGTGGT GGCCCTGCCA 
GTCCCTCGGC TTCAGGGTAT CCCCAAAGTG ACAGGGCGAG GCATGGTCTC CTACCATGCG 
CATAACGGGC CCGTTAAGTT CATCGTCTCA GCCACGGCGT TTCAAAACAA GGACAGAGCC 
AGGGACAGCC CACGTTCCGG TTCTGAGCTT CAGGATGAAG ACCCCAAGGA CTTGCTCTGC 
AGCGAGGAAG GTCCTTCCTG CCCGGGCCAG CCTGACACCT ACACATCCGT TTGGTTGGGA 
GACTCCCTAG GATTGCCAAC TCAAAAGAAT GATCTGTCTT CGTCCTCGGG GTCACTGAAC 
CTGTCCCATG GCTCCAGTTC CCTAGAGCAC AGATCTGTGG ACAGCAGTCT GTGTGACCTG 
CTGAGGGACC CCAGTGCCTC CCCAAGGAGC AGACCACAGG GCTCTAGGCG AGCAAGAGCC 
AGTTCAGCGC TGGTGGTATG TGGGGGCCAG GGCCACCGGC GGGTGCACAG GAAGGCCAGG 
CAGCCTTCTC AGGAGGACCT GGTTTCTTCA GTGATGGTGT GGCAGATCCC CCTGCTGGGC 
ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508836.1
CDS	194..2419
Translation	MLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPAPSSPGSPWAEFCLICVFLPCLPSGAYAISGTGCGAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_006508773.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508773.4

ORF Insert Sequence:

ATGCTGAATG ACGTGCTCAT GTGTGCCACC GCCAGCTCAC GGAACTCACA TGAAAGCCAT 
GCCGTGATGA GCCAGAGGTA CCTGCTCAAG TGGAGTGTCC CTCTAGGTCA CGTGGATGTG 
ATTCAGTACA ATGGAGGCTC TGGCGCCGGG GAGCACTGTC GGCACCACGC TGCACACTCA 
CCTGAGAGCC TGGCCGTGGT GGCCAACGCG AAGCCACACA AAGTTTACAT GGGGCCAGGG 
CAGCTGTACC AGGATCTGCA GAACCTACTG CACGACCTGA ACGTTGTTGG CCAGATCTCG 
CAGCTGATAG GAAACCTCCG AGGGAGCTAT CAGAACTTAA ACCAGTCTGT AGCCCATGAT 
TGGACGTCGG GCTTACAGCG GCTGATACTG AGGAAAGAAG ATGCGATCAG AGCTGCCGAC 
CGCTGCCGGA TCCAGTTACA GCTCCCGGGA AAGCAGGACA AGTCCGGGCG GCCCACGTTC 
TTCACTGCTG TGTTCAATAC GCTTACACCA GCCATCAAGG AGTCCTGGGT CAGCAGCCTG 
CAGATGGCCA AGCTGGCCCT TGAGGAGGAG AACCATATGG GATGGTTCTG TGTGGATGAC 
GATGGCAACC TAGCCAAGAA GGAGACACAC CCTCTCCTGG TGGGACACAT GCCTGTGATG 
GTGGCCAAGC AGCCAGAGTT CAAGATTGAA TGTGCAGCTT ATAACCCTGA ACCTTACCTG 
AGCAATGAAA GTCAACCAGC CTCACCCTCC ACAGCCCGTG GCTTTCTGTG GATTGGAAGC 
TGTAGCAACC AGATGGGTCA GGTCGCCATT GTCTCCTTCC AAGGTTCCAA CCCCAAAGTC 
ATCGAGTGCT TCAATGTGGA ATCACGCATC CTGTGTATGG TATACATTCC CGCAGAGGAG 
TCAAAGCCCC AAGAAACCAC CGAAACCAAG GATCCAGAAG CCACAGCCTC GAGAGCACCC 
CATGTGCCCA CCATCTGCCT GGGCACAGAA GAGGGAAGCA TTTCCATTTA CAAAAGCAGT 
CAGGGCTGCA AGAAGGTGAG GCTTCAGCAC TTCTATGCAC CGGACAAGTC CACGGTCATG 
AGCTTGGCCT GCTCCCCACA AGGCCTGTAT GCAGGCCTGG TCAATGGGTC CGTAGCCAGC 
TACACCAAGG CTCCAGCACC GAGCTCTCCT GGCAGCCCGT GGGCAGAGTT CTGTCTCATC 
TGCGTTTTCC TGCCATGCTT GCCCTCGGGA GCATATGCCA TCTCGGGTAC AGGCTGTGGT 
GCAGTGAAGG GCTATGGATC CTGGAACTCG GAACCCCAGC AAGTGATCAA GTTGGGTGTC 
CTGCCAGTCA GAAGCCTTCT CCTGGTGGAG GGTGCACTGT GGGCAGCCTC GGGAGGGCAG 
GTGTTCATGG CCAGCGTGGA GACTCACACT ATAGAGAATC AGCTGGAGGC CCACCAAGAT 
GAAGGGATGG TCATCTCCCA CATGGCTGTG GCTGGCGTTG GCATCTGGAT TGCCTTCACC 
TCGGGGTCCA CCCTCCGCCT CTTCCACACA GAGACCTTGA AGCACCTGCA GGATGTCAAC 
ATTGATGCCC CTGTGCATAG CATGCTGCCA GGGCACCAGC GTCTATCTGT GACCAGCCTG 
CTCGTCTGCC ACGGCTTGCT CATGGTCGGT ACCAGCCTGG GTGTCGTGGT GGCCCTGCCA 
GTCCCTCGGC TTCAGGGTAT CCCCAAAGTG ACAGGGCGAG GCATGGTCTC CTACCATGCG 
CATAACGGGC CCGTTAAGTT CATCGTCTCA GCCACGGCGT TTCAAAACAA GGACAGAGCC 
AGGGACAGCC CACGTTCCGG TTCTGAGCTT CAGGATGAAG ACCCCAAGGA CTTGCTCTGC 
AGCGAGGAAG GTCCTTCCTG CCCGGGCCAG CCTGACACCT ACACATCCGT TTGGTTGGGA 
GACTCCCTAG GATTGCCAAC TCAAAAGAAT GATCTGTCTT CGTCCTCGGG GTCACTGAAC 
CTGTCCCATG GCTCCAGTTC CCTAGAGCAC AGATCTGTGG ACAGCAGTCT GTGTGACCTG 
CTGAGGGACC CCAGTGCCTC CCCAAGGAGC AGACCACAGG GCTCTAGGCG AGCAAGAGCC 
AGTTCAGCGC TGGTGGTATG TGGGGGCCAG GGCCACCGGC GGGTGCACAG GAAGGCCAGG 
CAGCCTTCTC AGGAGGACCT GGTTTCTTCA GTGATGGTGT GGCAGATCCC CCTGCTGGGC 
ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_006508771.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508834.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X8, mRNA.

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CloneID	OMu40384
Clone ID Related Accession (Same CDS sequence)	XM_006508771.3 , XM_006508770.3 , XM_006508771.4 , XM_030243425.1 , XM_017312673.1
Accession Version	XM_006508771.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508771.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAACTCT CTCATGACCT AACCCGCTTT AAGGCGCACT GTGAGGAAAA GATGAGAGGC 
TTGGTGGCCA GTACGGTGGG AGCCATGGAG ATTCAGCAGG CAAAGCAGAG GCAGGAGCGG 
AAGATGCAGA AGCTTATGAA GGCAGCCAAG GAGGGCACCA AAGATGGGCT GGAGAAAACC 
AAAGCTGCTG TGAAGAGAGG CGGCTCCTTC ATCAGGACCA GGTCCTTGGT CTCTCAAGAT 
CACAGGTGTT ATTTTGAAGA AGAGCAGAAT TTGTTCATTG ACGTGGACTG CAAGCACCCG 
GAGGCTGTCC TCACCCCAAT GCCCGAGGGC TTGTCTCAGC AGCAGGTTGT GAGAAGATAC 
ATTCTGGGCT CCATTGTTGA GAGCGAGAAG AACTATGTAG ATGCTCTCCG GAGGATTTTG 
GAGCAATATG AGAAGCCGCT GTCTGAGATG GAGCCCCGGC TCCTGAGTGA CAGGAAGCTC 
AGGATGGTTT TCTACCGCGT CAAGGAGATC CTGCAGTGTC ACTCCATGTT CCAGATTGCG 
CTGGCCAGCC GCGTGTCCGA GTGGGATGTC GTGGAAACAA TCGGCGACGT TTTTGTGGCC 
TCGTTCTCCA AGTCCATGGT GCTGGATGCT TACAGCGAGT ACGTGAACAA CTTCAGCACG 
GCCGTGGCTG TCCTCAAGAA GACGTGTGCC ACAAAGCCTG CTTTCCTTGA GTTCTTAAAG 
CTGAGCCAGG ATTCCAGTCC AGACCGAGTC ACACTCCACA GCCTTATGAT GCGGCCCATC 
CAGAGGTTCC CACAGTTCAT CCTCCTACTG CAGGACATGC TGAAGAACAC CGCCAAAGGA 
CATCCTGACA GGCTGCCCCT TCAGATGGCG CTGACGGAGC TCGAGACCCT GGCAGAGAAG 
CTAAACGAGA GAAAAAGAGA TGCCGACCAG CGATGCGAAA TCAAACAAAT AGCAAAAGCC 
ATAAACGAGA GATACCTGAA CAAGCTCCTC AGCAGTGGAA ACCGGTACCT CGTCCGGTCC 
GATGACGTCA TCGAGACGGT TTACAACGAC CGGGGAGAAA TCGTCAAGAC CAAACAACGC 
CGGATCTTCA TGCTGAATGA CGTGCTCATG TGTGCCACCG CCAGCTCACG GAACTCACAT 
GAAAGCCATG CCGTGATGAG CCAGAGGTAC CTGCTCAAGT GGAGTGTCCC TCTAGGTCAC 
GTGGATGTGA TTCAGTACAA TGGAGGCTCT GGCGCCGGGG AGCACTGTCG GCACCACGCT 
GCACACTCAC CTGAGAGCCT GGCCGTGGTG GCCAACGCGA AGCCACACAA AGTTTACATG 
GGGCCAGGGC AGCTGTACCA GGATCTGCAG AACCTACTGC ACGACCTGAA CGTTGTTGGC 
CAGATCTCGC AGCTGATAGG AAACCTCCGA GGGAGCTATC AGAACTTAAA CCAGTCTGTA 
GCCCATGATT GGACGTCGGG CTTACAGCGG CTGATACTGA GGAAAGAAGA TGCGATCAGA 
GCTGCCGACC GCTGCCGGAT CCAGTTACAG CTCCCGGGAA AGCAGGACAA GTCCGGGCGG 
CCCACGTTCT TCACTGCTGT GTTCAATACG CTTACACCAG CCATCAAGGA GTCCTGGGTC 
AGCAGCCTGC AGATGGCCAA GCTGGCCCTT GAGGAGGAGA ACCATATGGG ATGGTTCTGT 
GTGGATGACG ATGGCAACCT AGCCAAGAAG GAGACACACC CTCTCCTGGT GGGACACATG 
CCTGTGATGG TGGCCAAGCA GCCAGAGTTC AAGATTGAAT GTGCAGCTTA TAACCCTGAA 
CCTTACCTGA GCAATGAAAG TCAACCAGCC TCACCCTCCA CAGCCCGTGG CTTTCTGTGG 
ATTGGAAGCT GTAGCAACCA GATGGGTCAG GTCGCCATTG TCTCCTTCCA AGGTTCCAAC 
CCCAAAGTCA TCGAGTGCTT CAATGTGGAA TCACGCATCC TGTGTATGGT ATACATTCCC 
GCAGAGGAGT CAAAGCCCCA AGAAACCACC GAAACCAAGG ATCCAGAAGC CACAGCCTCG 
AGAGCACCCC ATGTGCCCAC CATCTGCCTG GGCACAGAAG AGGGAAGCAT TTCCATTTAC 
AAAAGCAGTC AGGGCTGCAA GAAGGTGAGG CTTCAGCACT TCTATGCACC GGACAAGTCC 
ACGGTCATGA GCTTGGCCTG CTCCCCACAA GGCCTGTATG CAGGCCTGGT CAATGGGTCC 
GTAGCCAGCT ACACCAAGGC TCCAGCACCG AGCTCTCCTG GCAGCCCGTG GGCAGAGTTC 
TGTCTCATCT GCGTTTTCCT GCCATGCTTG CCCTCGGGAG CATATGCCAT CTCGGGTACA 
GGCTGTGGTG CAGTGAAGGG CTATGGATCC TGGAACTCGG AACCCCAGCA AGTGATCAAG 
TTGGGTGTCC TGCCAGTCAG AAGCCTTCTC CTGGTGGAGG GTGCACTGTG GGCAGCCTCG 
GGAGGGCAGG TGTTCATGGC CAGCGTGGAG ACTCACACTA TAGAGAATCA GCTGGAGGCC 
CACCAAGATG AAGGGATGGT CATCTCCCAC ATGGCTGTGG CTGGCGTTGG CATCTGGATT 
GCCTTCACCT CGGGGTCCAC CCTCCGCCTC TTCCACACAG AGACCTTGAA GCACCTGCAG 
GATGTCAACA TTGATGCCCC TGTGCATAGC ATGCTGCCAG GGCACCAGCG TCTATCTGTG 
ACCAGCCTGC TCGTCTGCCA CGGCTTGCTC ATGGTCGGTA CCAGCCTGGG TGTCGTGGTG 
GCCCTGCCAG TCCCTCGGCT TCAGGGTATC CCCAAAGTGA CAGGGCGAGG CATGGTCTCC 
TACCATGCGC ATAACGGGCC CGTTAAGTTC ATCGTCTCAG CCACGGCGTT TCAAAACAAG 
GACAGAGCCA GGGACAGCCC ACGTTCCGGT TCTGAGCTTC AGGATGAAGA CCCCAAGGAC 
TTGCTCTGCA GCGAGGAAGG TCCTTCCTGC CCGGGCCAGC CTGACACCTA CACATCCGTT 
TGGTTGGGAG ACTCCCTAGG ATTGCCAACT CAAAAGAATG ATCTGTCTTC GTCCTCGGGG 
TCACTGAACC TGTCCCATGG CTCCAGTTCC CTAGAGCACA GATCTGTGGA CAGCAGTCTG 
TGTGACCTGC TGAGGGACCC CAGTGCCTCC CCAAGGAGCA GACCACAGGG CTCTAGGCGA 
GCAAGAGCCA GTTCAGCGCT GGTGGTATGT GGGGGCCAGG GCCACCGGCG GGTGCACAGG 
AAGGCCAGGC AGCCTTCTCA GGAGGACCTG GTTTCTTCAG TGATGGTGTG GCAGATCCCC 
CTGCTGGGCA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508834.1
CDS	592..4746
Translation	MEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQLSHDLTRFKAHCEEKMRGLVASTVGAMEIQQAKQRQERKMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPAPSSPGSPWAEFCLICVFLPCLPSGAYAISGTGCGAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_006508771.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508771.4

ORF Insert Sequence:

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAACTCT CTCATGACCT AACCCGCTTT AAGGCGCACT GTGAGGAAAA GATGAGAGGC 
TTGGTGGCCA GTACGGTGGG AGCCATGGAG ATTCAGCAGG CAAAGCAGAG GCAGGAGCGG 
AAGATGCAGA AGCTTATGAA GGCAGCCAAG GAGGGCACCA AAGATGGGCT GGAGAAAACC 
AAAGCTGCTG TGAAGAGAGG CGGCTCCTTC ATCAGGACCA GGTCCTTGGT CTCTCAAGAT 
CACAGGTGTT ATTTTGAAGA AGAGCAGAAT TTGTTCATTG ACGTGGACTG CAAGCACCCG 
GAGGCTGTCC TCACCCCAAT GCCCGAGGGC TTGTCTCAGC AGCAGGTTGT GAGAAGATAC 
ATTCTGGGCT CCATTGTTGA GAGCGAGAAG AACTATGTAG ATGCTCTCCG GAGGATTTTG 
GAGCAATATG AGAAGCCGCT GTCTGAGATG GAGCCCCGGC TCCTGAGTGA CAGGAAGCTC 
AGGATGGTTT TCTACCGCGT CAAGGAGATC CTGCAGTGTC ACTCCATGTT CCAGATTGCG 
CTGGCCAGCC GCGTGTCCGA GTGGGATGTC GTGGAAACAA TCGGCGACGT TTTTGTGGCC 
TCGTTCTCCA AGTCCATGGT GCTGGATGCT TACAGCGAGT ACGTGAACAA CTTCAGCACG 
GCCGTGGCTG TCCTCAAGAA GACGTGTGCC ACAAAGCCTG CTTTCCTTGA GTTCTTAAAG 
CTGAGCCAGG ATTCCAGTCC AGACCGAGTC ACACTCCACA GCCTTATGAT GCGGCCCATC 
CAGAGGTTCC CACAGTTCAT CCTCCTACTG CAGGACATGC TGAAGAACAC CGCCAAAGGA 
CATCCTGACA GGCTGCCCCT TCAGATGGCG CTGACGGAGC TCGAGACCCT GGCAGAGAAG 
CTAAACGAGA GAAAAAGAGA TGCCGACCAG CGATGCGAAA TCAAACAAAT AGCAAAAGCC 
ATAAACGAGA GATACCTGAA CAAGCTCCTC AGCAGTGGAA ACCGGTACCT CGTCCGGTCC 
GATGACGTCA TCGAGACGGT TTACAACGAC CGGGGAGAAA TCGTCAAGAC CAAACAACGC 
CGGATCTTCA TGCTGAATGA CGTGCTCATG TGTGCCACCG CCAGCTCACG GAACTCACAT 
GAAAGCCATG CCGTGATGAG CCAGAGGTAC CTGCTCAAGT GGAGTGTCCC TCTAGGTCAC 
GTGGATGTGA TTCAGTACAA TGGAGGCTCT GGCGCCGGGG AGCACTGTCG GCACCACGCT 
GCACACTCAC CTGAGAGCCT GGCCGTGGTG GCCAACGCGA AGCCACACAA AGTTTACATG 
GGGCCAGGGC AGCTGTACCA GGATCTGCAG AACCTACTGC ACGACCTGAA CGTTGTTGGC 
CAGATCTCGC AGCTGATAGG AAACCTCCGA GGGAGCTATC AGAACTTAAA CCAGTCTGTA 
GCCCATGATT GGACGTCGGG CTTACAGCGG CTGATACTGA GGAAAGAAGA TGCGATCAGA 
GCTGCCGACC GCTGCCGGAT CCAGTTACAG CTCCCGGGAA AGCAGGACAA GTCCGGGCGG 
CCCACGTTCT TCACTGCTGT GTTCAATACG CTTACACCAG CCATCAAGGA GTCCTGGGTC 
AGCAGCCTGC AGATGGCCAA GCTGGCCCTT GAGGAGGAGA ACCATATGGG ATGGTTCTGT 
GTGGATGACG ATGGCAACCT AGCCAAGAAG GAGACACACC CTCTCCTGGT GGGACACATG 
CCTGTGATGG TGGCCAAGCA GCCAGAGTTC AAGATTGAAT GTGCAGCTTA TAACCCTGAA 
CCTTACCTGA GCAATGAAAG TCAACCAGCC TCACCCTCCA CAGCCCGTGG CTTTCTGTGG 
ATTGGAAGCT GTAGCAACCA GATGGGTCAG GTCGCCATTG TCTCCTTCCA AGGTTCCAAC 
CCCAAAGTCA TCGAGTGCTT CAATGTGGAA TCACGCATCC TGTGTATGGT ATACATTCCC 
GCAGAGGAGT CAAAGCCCCA AGAAACCACC GAAACCAAGG ATCCAGAAGC CACAGCCTCG 
AGAGCACCCC ATGTGCCCAC CATCTGCCTG GGCACAGAAG AGGGAAGCAT TTCCATTTAC 
AAAAGCAGTC AGGGCTGCAA GAAGGTGAGG CTTCAGCACT TCTATGCACC GGACAAGTCC 
ACGGTCATGA GCTTGGCCTG CTCCCCACAA GGCCTGTATG CAGGCCTGGT CAATGGGTCC 
GTAGCCAGCT ACACCAAGGC TCCAGCACCG AGCTCTCCTG GCAGCCCGTG GGCAGAGTTC 
TGTCTCATCT GCGTTTTCCT GCCATGCTTG CCCTCGGGAG CATATGCCAT CTCGGGTACA 
GGCTGTGGTG CAGTGAAGGG CTATGGATCC TGGAACTCGG AACCCCAGCA AGTGATCAAG 
TTGGGTGTCC TGCCAGTCAG AAGCCTTCTC CTGGTGGAGG GTGCACTGTG GGCAGCCTCG 
GGAGGGCAGG TGTTCATGGC CAGCGTGGAG ACTCACACTA TAGAGAATCA GCTGGAGGCC 
CACCAAGATG AAGGGATGGT CATCTCCCAC ATGGCTGTGG CTGGCGTTGG CATCTGGATT 
GCCTTCACCT CGGGGTCCAC CCTCCGCCTC TTCCACACAG AGACCTTGAA GCACCTGCAG 
GATGTCAACA TTGATGCCCC TGTGCATAGC ATGCTGCCAG GGCACCAGCG TCTATCTGTG 
ACCAGCCTGC TCGTCTGCCA CGGCTTGCTC ATGGTCGGTA CCAGCCTGGG TGTCGTGGTG 
GCCCTGCCAG TCCCTCGGCT TCAGGGTATC CCCAAAGTGA CAGGGCGAGG CATGGTCTCC 
TACCATGCGC ATAACGGGCC CGTTAAGTTC ATCGTCTCAG CCACGGCGTT TCAAAACAAG 
GACAGAGCCA GGGACAGCCC ACGTTCCGGT TCTGAGCTTC AGGATGAAGA CCCCAAGGAC 
TTGCTCTGCA GCGAGGAAGG TCCTTCCTGC CCGGGCCAGC CTGACACCTA CACATCCGTT 
TGGTTGGGAG ACTCCCTAGG ATTGCCAACT CAAAAGAATG ATCTGTCTTC GTCCTCGGGG 
TCACTGAACC TGTCCCATGG CTCCAGTTCC CTAGAGCACA GATCTGTGGA CAGCAGTCTG 
TGTGACCTGC TGAGGGACCC CAGTGCCTCC CCAAGGAGCA GACCACAGGG CTCTAGGCGA 
GCAAGAGCCA GTTCAGCGCT GGTGGTATGT GGGGGCCAGG GCCACCGGCG GGTGCACAGG 
AAGGCCAGGC AGCCTTCTCA GGAGGACCTG GTTTCTTCAG TGATGGTGTG GCAGATCCCC 
CTGCTGGGCA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_030243425.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099285.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu40384
Clone ID Related Accession (Same CDS sequence)	XM_006508771.3 , XM_006508770.3 , XM_006508771.4 , XM_030243425.1 , XM_017312673.1
Accession Version	XM_030243425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAACTCT CTCATGACCT AACCCGCTTT AAGGCGCACT GTGAGGAAAA GATGAGAGGC 
TTGGTGGCCA GTACGGTGGG AGCCATGGAG ATTCAGCAGG CAAAGCAGAG GCAGGAGCGG 
AAGATGCAGA AGCTTATGAA GGCAGCCAAG GAGGGCACCA AAGATGGGCT GGAGAAAACC 
AAAGCTGCTG TGAAGAGAGG CGGCTCCTTC ATCAGGACCA GGTCCTTGGT CTCTCAAGAT 
CACAGGTGTT ATTTTGAAGA AGAGCAGAAT TTGTTCATTG ACGTGGACTG CAAGCACCCG 
GAGGCTGTCC TCACCCCAAT GCCCGAGGGC TTGTCTCAGC AGCAGGTTGT GAGAAGATAC 
ATTCTGGGCT CCATTGTTGA GAGCGAGAAG AACTATGTAG ATGCTCTCCG GAGGATTTTG 
GAGCAATATG AGAAGCCGCT GTCTGAGATG GAGCCCCGGC TCCTGAGTGA CAGGAAGCTC 
AGGATGGTTT TCTACCGCGT CAAGGAGATC CTGCAGTGTC ACTCCATGTT CCAGATTGCG 
CTGGCCAGCC GCGTGTCCGA GTGGGATGTC GTGGAAACAA TCGGCGACGT TTTTGTGGCC 
TCGTTCTCCA AGTCCATGGT GCTGGATGCT TACAGCGAGT ACGTGAACAA CTTCAGCACG 
GCCGTGGCTG TCCTCAAGAA GACGTGTGCC ACAAAGCCTG CTTTCCTTGA GTTCTTAAAG 
CTGAGCCAGG ATTCCAGTCC AGACCGAGTC ACACTCCACA GCCTTATGAT GCGGCCCATC 
CAGAGGTTCC CACAGTTCAT CCTCCTACTG CAGGACATGC TGAAGAACAC CGCCAAAGGA 
CATCCTGACA GGCTGCCCCT TCAGATGGCG CTGACGGAGC TCGAGACCCT GGCAGAGAAG 
CTAAACGAGA GAAAAAGAGA TGCCGACCAG CGATGCGAAA TCAAACAAAT AGCAAAAGCC 
ATAAACGAGA GATACCTGAA CAAGCTCCTC AGCAGTGGAA ACCGGTACCT CGTCCGGTCC 
GATGACGTCA TCGAGACGGT TTACAACGAC CGGGGAGAAA TCGTCAAGAC CAAACAACGC 
CGGATCTTCA TGCTGAATGA CGTGCTCATG TGTGCCACCG CCAGCTCACG GAACTCACAT 
GAAAGCCATG CCGTGATGAG CCAGAGGTAC CTGCTCAAGT GGAGTGTCCC TCTAGGTCAC 
GTGGATGTGA TTCAGTACAA TGGAGGCTCT GGCGCCGGGG AGCACTGTCG GCACCACGCT 
GCACACTCAC CTGAGAGCCT GGCCGTGGTG GCCAACGCGA AGCCACACAA AGTTTACATG 
GGGCCAGGGC AGCTGTACCA GGATCTGCAG AACCTACTGC ACGACCTGAA CGTTGTTGGC 
CAGATCTCGC AGCTGATAGG AAACCTCCGA GGGAGCTATC AGAACTTAAA CCAGTCTGTA 
GCCCATGATT GGACGTCGGG CTTACAGCGG CTGATACTGA GGAAAGAAGA TGCGATCAGA 
GCTGCCGACC GCTGCCGGAT CCAGTTACAG CTCCCGGGAA AGCAGGACAA GTCCGGGCGG 
CCCACGTTCT TCACTGCTGT GTTCAATACG CTTACACCAG CCATCAAGGA GTCCTGGGTC 
AGCAGCCTGC AGATGGCCAA GCTGGCCCTT GAGGAGGAGA ACCATATGGG ATGGTTCTGT 
GTGGATGACG ATGGCAACCT AGCCAAGAAG GAGACACACC CTCTCCTGGT GGGACACATG 
CCTGTGATGG TGGCCAAGCA GCCAGAGTTC AAGATTGAAT GTGCAGCTTA TAACCCTGAA 
CCTTACCTGA GCAATGAAAG TCAACCAGCC TCACCCTCCA CAGCCCGTGG CTTTCTGTGG 
ATTGGAAGCT GTAGCAACCA GATGGGTCAG GTCGCCATTG TCTCCTTCCA AGGTTCCAAC 
CCCAAAGTCA TCGAGTGCTT CAATGTGGAA TCACGCATCC TGTGTATGGT ATACATTCCC 
GCAGAGGAGT CAAAGCCCCA AGAAACCACC GAAACCAAGG ATCCAGAAGC CACAGCCTCG 
AGAGCACCCC ATGTGCCCAC CATCTGCCTG GGCACAGAAG AGGGAAGCAT TTCCATTTAC 
AAAAGCAGTC AGGGCTGCAA GAAGGTGAGG CTTCAGCACT TCTATGCACC GGACAAGTCC 
ACGGTCATGA GCTTGGCCTG CTCCCCACAA GGCCTGTATG CAGGCCTGGT CAATGGGTCC 
GTAGCCAGCT ACACCAAGGC TCCAGCACCG AGCTCTCCTG GCAGCCCGTG GGCAGAGTTC 
TGTCTCATCT GCGTTTTCCT GCCATGCTTG CCCTCGGGAG CATATGCCAT CTCGGGTACA 
GGCTGTGGTG CAGTGAAGGG CTATGGATCC TGGAACTCGG AACCCCAGCA AGTGATCAAG 
TTGGGTGTCC TGCCAGTCAG AAGCCTTCTC CTGGTGGAGG GTGCACTGTG GGCAGCCTCG 
GGAGGGCAGG TGTTCATGGC CAGCGTGGAG ACTCACACTA TAGAGAATCA GCTGGAGGCC 
CACCAAGATG AAGGGATGGT CATCTCCCAC ATGGCTGTGG CTGGCGTTGG CATCTGGATT 
GCCTTCACCT CGGGGTCCAC CCTCCGCCTC TTCCACACAG AGACCTTGAA GCACCTGCAG 
GATGTCAACA TTGATGCCCC TGTGCATAGC ATGCTGCCAG GGCACCAGCG TCTATCTGTG 
ACCAGCCTGC TCGTCTGCCA CGGCTTGCTC ATGGTCGGTA CCAGCCTGGG TGTCGTGGTG 
GCCCTGCCAG TCCCTCGGCT TCAGGGTATC CCCAAAGTGA CAGGGCGAGG CATGGTCTCC 
TACCATGCGC ATAACGGGCC CGTTAAGTTC ATCGTCTCAG CCACGGCGTT TCAAAACAAG 
GACAGAGCCA GGGACAGCCC ACGTTCCGGT TCTGAGCTTC AGGATGAAGA CCCCAAGGAC 
TTGCTCTGCA GCGAGGAAGG TCCTTCCTGC CCGGGCCAGC CTGACACCTA CACATCCGTT 
TGGTTGGGAG ACTCCCTAGG ATTGCCAACT CAAAAGAATG ATCTGTCTTC GTCCTCGGGG 
TCACTGAACC TGTCCCATGG CTCCAGTTCC CTAGAGCACA GATCTGTGGA CAGCAGTCTG 
TGTGACCTGC TGAGGGACCC CAGTGCCTCC CCAAGGAGCA GACCACAGGG CTCTAGGCGA 
GCAAGAGCCA GTTCAGCGCT GGTGGTATGT GGGGGCCAGG GCCACCGGCG GGTGCACAGG 
AAGGCCAGGC AGCCTTCTCA GGAGGACCTG GTTTCTTCAG TGATGGTGTG GCAGATCCCC 
CTGCTGGGCA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099285.1
CDS	255..4409
Translation	MEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQLSHDLTRFKAHCEEKMRGLVASTVGAMEIQQAKQRQERKMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPAPSSPGSPWAEFCLICVFLPCLPSGAYAISGTGCGAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_030243425.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243425.1

ORF Insert Sequence:

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAACTCT CTCATGACCT AACCCGCTTT AAGGCGCACT GTGAGGAAAA GATGAGAGGC 
TTGGTGGCCA GTACGGTGGG AGCCATGGAG ATTCAGCAGG CAAAGCAGAG GCAGGAGCGG 
AAGATGCAGA AGCTTATGAA GGCAGCCAAG GAGGGCACCA AAGATGGGCT GGAGAAAACC 
AAAGCTGCTG TGAAGAGAGG CGGCTCCTTC ATCAGGACCA GGTCCTTGGT CTCTCAAGAT 
CACAGGTGTT ATTTTGAAGA AGAGCAGAAT TTGTTCATTG ACGTGGACTG CAAGCACCCG 
GAGGCTGTCC TCACCCCAAT GCCCGAGGGC TTGTCTCAGC AGCAGGTTGT GAGAAGATAC 
ATTCTGGGCT CCATTGTTGA GAGCGAGAAG AACTATGTAG ATGCTCTCCG GAGGATTTTG 
GAGCAATATG AGAAGCCGCT GTCTGAGATG GAGCCCCGGC TCCTGAGTGA CAGGAAGCTC 
AGGATGGTTT TCTACCGCGT CAAGGAGATC CTGCAGTGTC ACTCCATGTT CCAGATTGCG 
CTGGCCAGCC GCGTGTCCGA GTGGGATGTC GTGGAAACAA TCGGCGACGT TTTTGTGGCC 
TCGTTCTCCA AGTCCATGGT GCTGGATGCT TACAGCGAGT ACGTGAACAA CTTCAGCACG 
GCCGTGGCTG TCCTCAAGAA GACGTGTGCC ACAAAGCCTG CTTTCCTTGA GTTCTTAAAG 
CTGAGCCAGG ATTCCAGTCC AGACCGAGTC ACACTCCACA GCCTTATGAT GCGGCCCATC 
CAGAGGTTCC CACAGTTCAT CCTCCTACTG CAGGACATGC TGAAGAACAC CGCCAAAGGA 
CATCCTGACA GGCTGCCCCT TCAGATGGCG CTGACGGAGC TCGAGACCCT GGCAGAGAAG 
CTAAACGAGA GAAAAAGAGA TGCCGACCAG CGATGCGAAA TCAAACAAAT AGCAAAAGCC 
ATAAACGAGA GATACCTGAA CAAGCTCCTC AGCAGTGGAA ACCGGTACCT CGTCCGGTCC 
GATGACGTCA TCGAGACGGT TTACAACGAC CGGGGAGAAA TCGTCAAGAC CAAACAACGC 
CGGATCTTCA TGCTGAATGA CGTGCTCATG TGTGCCACCG CCAGCTCACG GAACTCACAT 
GAAAGCCATG CCGTGATGAG CCAGAGGTAC CTGCTCAAGT GGAGTGTCCC TCTAGGTCAC 
GTGGATGTGA TTCAGTACAA TGGAGGCTCT GGCGCCGGGG AGCACTGTCG GCACCACGCT 
GCACACTCAC CTGAGAGCCT GGCCGTGGTG GCCAACGCGA AGCCACACAA AGTTTACATG 
GGGCCAGGGC AGCTGTACCA GGATCTGCAG AACCTACTGC ACGACCTGAA CGTTGTTGGC 
CAGATCTCGC AGCTGATAGG AAACCTCCGA GGGAGCTATC AGAACTTAAA CCAGTCTGTA 
GCCCATGATT GGACGTCGGG CTTACAGCGG CTGATACTGA GGAAAGAAGA TGCGATCAGA 
GCTGCCGACC GCTGCCGGAT CCAGTTACAG CTCCCGGGAA AGCAGGACAA GTCCGGGCGG 
CCCACGTTCT TCACTGCTGT GTTCAATACG CTTACACCAG CCATCAAGGA GTCCTGGGTC 
AGCAGCCTGC AGATGGCCAA GCTGGCCCTT GAGGAGGAGA ACCATATGGG ATGGTTCTGT 
GTGGATGACG ATGGCAACCT AGCCAAGAAG GAGACACACC CTCTCCTGGT GGGACACATG 
CCTGTGATGG TGGCCAAGCA GCCAGAGTTC AAGATTGAAT GTGCAGCTTA TAACCCTGAA 
CCTTACCTGA GCAATGAAAG TCAACCAGCC TCACCCTCCA CAGCCCGTGG CTTTCTGTGG 
ATTGGAAGCT GTAGCAACCA GATGGGTCAG GTCGCCATTG TCTCCTTCCA AGGTTCCAAC 
CCCAAAGTCA TCGAGTGCTT CAATGTGGAA TCACGCATCC TGTGTATGGT ATACATTCCC 
GCAGAGGAGT CAAAGCCCCA AGAAACCACC GAAACCAAGG ATCCAGAAGC CACAGCCTCG 
AGAGCACCCC ATGTGCCCAC CATCTGCCTG GGCACAGAAG AGGGAAGCAT TTCCATTTAC 
AAAAGCAGTC AGGGCTGCAA GAAGGTGAGG CTTCAGCACT TCTATGCACC GGACAAGTCC 
ACGGTCATGA GCTTGGCCTG CTCCCCACAA GGCCTGTATG CAGGCCTGGT CAATGGGTCC 
GTAGCCAGCT ACACCAAGGC TCCAGCACCG AGCTCTCCTG GCAGCCCGTG GGCAGAGTTC 
TGTCTCATCT GCGTTTTCCT GCCATGCTTG CCCTCGGGAG CATATGCCAT CTCGGGTACA 
GGCTGTGGTG CAGTGAAGGG CTATGGATCC TGGAACTCGG AACCCCAGCA AGTGATCAAG 
TTGGGTGTCC TGCCAGTCAG AAGCCTTCTC CTGGTGGAGG GTGCACTGTG GGCAGCCTCG 
GGAGGGCAGG TGTTCATGGC CAGCGTGGAG ACTCACACTA TAGAGAATCA GCTGGAGGCC 
CACCAAGATG AAGGGATGGT CATCTCCCAC ATGGCTGTGG CTGGCGTTGG CATCTGGATT 
GCCTTCACCT CGGGGTCCAC CCTCCGCCTC TTCCACACAG AGACCTTGAA GCACCTGCAG 
GATGTCAACA TTGATGCCCC TGTGCATAGC ATGCTGCCAG GGCACCAGCG TCTATCTGTG 
ACCAGCCTGC TCGTCTGCCA CGGCTTGCTC ATGGTCGGTA CCAGCCTGGG TGTCGTGGTG 
GCCCTGCCAG TCCCTCGGCT TCAGGGTATC CCCAAAGTGA CAGGGCGAGG CATGGTCTCC 
TACCATGCGC ATAACGGGCC CGTTAAGTTC ATCGTCTCAG CCACGGCGTT TCAAAACAAG 
GACAGAGCCA GGGACAGCCC ACGTTCCGGT TCTGAGCTTC AGGATGAAGA CCCCAAGGAC 
TTGCTCTGCA GCGAGGAAGG TCCTTCCTGC CCGGGCCAGC CTGACACCTA CACATCCGTT 
TGGTTGGGAG ACTCCCTAGG ATTGCCAACT CAAAAGAATG ATCTGTCTTC GTCCTCGGGG 
TCACTGAACC TGTCCCATGG CTCCAGTTCC CTAGAGCACA GATCTGTGGA CAGCAGTCTG 
TGTGACCTGC TGAGGGACCC CAGTGCCTCC CCAAGGAGCA GACCACAGGG CTCTAGGCGA 
GCAAGAGCCA GTTCAGCGCT GGTGGTATGT GGGGGCCAGG GCCACCGGCG GGTGCACAGG 
AAGGCCAGGC AGCCTTCTCA GGAGGACCTG GTTTCTTCAG TGATGGTGTG GCAGATCCCC 
CTGCTGGGCA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_030243426.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099286.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X12, mRNA.

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CloneID	OMu73730
Clone ID Related Accession (Same CDS sequence)	XM_030243426.1
Accession Version	XM_030243426.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAAATGC AGAAGCTTAT GAAGGCAGCC AAGGAGGGCA CCAAAGATGG GCTGGAGAAA 
ACCAAAGCTG CTGTGAAGAG AGGCGGCTCC TTCATCAGGA CCAGGTCCTT GGTCTCTCAA 
GATCACAGGT GTTATTTTGA AGAAGAGCAG AATTTGTTCA TTGACGTGGA CTGCAAGCAC 
CCGGAGGCTG TCCTCACCCC AATGCCCGAG GGCTTGTCTC AGCAGCAGGT TGTGAGAAGA 
TACATTCTGG GCTCCATTGT TGAGAGCGAG AAGAACTATG TAGATGCTCT CCGGAGGATT 
TTGGAGCAAT ATGAGAAGCC GCTGTCTGAG ATGGAGCCCC GGCTCCTGAG TGACAGGAAG 
CTCAGGATGG TTTTCTACCG CGTCAAGGAG ATCCTGCAGT GTCACTCCAT GTTCCAGATT 
GCGCTGGCCA GCCGCGTGTC CGAGTGGGAT GTCGTGGAAA CAATCGGCGA CGTTTTTGTG 
GCCTCGTTCT CCAAGTCCAT GGTGCTGGAT GCTTACAGCG AGTACGTGAA CAACTTCAGC 
ACGGCCGTGG CTGTCCTCAA GAAGACGTGT GCCACAAAGC CTGCTTTCCT TGAGTTCTTA 
AAGCTGAGCC AGGATTCCAG TCCAGACCGA GTCACACTCC ACAGCCTTAT GATGCGGCCC 
ATCCAGAGGT TCCCACAGTT CATCCTCCTA CTGCAGGACA TGCTGAAGAA CACCGCCAAA 
GGACATCCTG ACAGGCTGCC CCTTCAGATG GCGCTGACGG AGCTCGAGAC CCTGGCAGAG 
AAGCTAAACG AGAGAAAAAG AGATGCCGAC CAGCGATGCG AAATCAAACA AATAGCAAAA 
GCCATAAACG AGAGATACCT GAACAAGCTC CTCAGCAGTG GAAACCGGTA CCTCGTCCGG 
TCCGATGACG TCATCGAGAC GGTTTACAAC GACCGGGGAG AAATCGTCAA GACCAAACAA 
CGCCGGATCT TCATGCTGAA TGACGTGCTC ATGTGTGCCA CCGCCAGCTC ACGGAACTCA 
CATGAAAGCC ATGCCGTGAT GAGCCAGAGG TACCTGCTCA AGTGGAGTGT CCCTCTAGGT 
CACGTGGATG TGATTCAGTA CAATGGAGGC TCTGGCGCCG GGGAGCACTG TCGGCACCAC 
GCTGCACACT CACCTGAGAG CCTGGCCGTG GTGGCCAACG CGAAGCCACA CAAAGTTTAC 
ATGGGGCCAG GGCAGCTGTA CCAGGATCTG CAGAACCTAC TGCACGACCT GAACGTTGTT 
GGCCAGATCT CGCAGCTGAT AGGAAACCTC CGAGGGAGCT ATCAGAACTT AAACCAGTCT 
GTAGCCCATG ATTGGACGTC GGGCTTACAG CGGCTGATAC TGAGGAAAGA AGATGCGATC 
AGAGCTGCCG ACCGCTGCCG GATCCAGTTA CAGCTCCCGG GAAAGCAGGA CAAGTCCGGG 
CGGCCCACGT TCTTCACTGC TGTGTTCAAT ACGCTTACAC CAGCCATCAA GGAGTCCTGG 
GTCAGCAGCC TGCAGATGGC CAAGCTGGCC CTTGAGGAGG AGAACCATAT GGGATGGTTC 
TGTGTGGATG ACGATGGCAA CCTAGCCAAG AAGGAGACAC ACCCTCTCCT GGTGGGACAC 
ATGCCTGTGA TGGTGGCCAA GCAGCCAGAG TTCAAGATTG AATGTGCAGC TTATAACCCT 
GAACCTTACC TGAGCAATGA AAGTCAACCA GCCTCACCCT CCACAGCCCG TGGCTTTCTG 
TGGATTGGAA GCTGTAGCAA CCAGATGGGT CAGGTCGCCA TTGTCTCCTT CCAAGGTTCC 
AACCCCAAAG TCATCGAGTG CTTCAATGTG GAATCACGCA TCCTGTGTAT GGTATACATT 
CCCGCAGAGG AGTCAAAGCC CCAAGAAACC ACCGAAACCA AGGATCCAGA AGCCACAGCC 
TCGAGAGCAC CCCATGTGCC CACCATCTGC CTGGGCACAG AAGAGGGAAG CATTTCCATT 
TACAAAAGCA GTCAGGGCTG CAAGAAGGTG AGGCTTCAGC ACTTCTATGC ACCGGACAAG 
TCCACGGTCA TGAGCTTGGC CTGCTCCCCA CAAGGCCTGT ATGCAGGCCT GGTCAATGGG 
TCCGTAGCCA GCTACACCAA GGCTCCAGCA CCGAGCTCTC CTGGCAGCCC GTGGGCAGAG 
TTCTGTCTCA TCTGCGTTTT CCTGCCATGC TTGCCCTCGG GAGCATATGC CATCTCGGGT 
ACAGGCTGTG GTGCAGTGAA GGGCTATGGA TCCTGGAACT CGGAACCCCA GCAAGTGATC 
AAGTTGGGTG TCCTGCCAGT CAGAAGCCTT CTCCTGGTGG AGGGTGCACT GTGGGCAGCC 
TCGGGAGGGC AGGTGTTCAT GGCCAGCGTG GAGACTCACA CTATAGAGAA TCAGCTGGAG 
GCCCACCAAG ATGAAGGGAT GGTCATCTCC CACATGGCTG TGGCTGGCGT TGGCATCTGG 
ATTGCCTTCA CCTCGGGGTC CACCCTCCGC CTCTTCCACA CAGAGACCTT GAAGCACCTG 
CAGGATGTCA ACATTGATGC CCCTGTGCAT AGCATGCTGC CAGGGCACCA GCGTCTATCT 
GTGACCAGCC TGCTCGTCTG CCACGGCTTG CTCATGGTCG GTACCAGCCT GGGTGTCGTG 
GTGGCCCTGC CAGTCCCTCG GCTTCAGGGT ATCCCCAAAG TGACAGGGCG AGGCATGGTC 
TCCTACCATG CGCATAACGG GCCCGTTAAG TTCATCGTCT CAGCCACGGC GTTTCAAAAC 
AAGGACAGAG CCAGGGACAG CCCACGTTCC GGTTCTGAGC TTCAGGATGA AGACCCCAAG 
GACTTGCTCT GCAGCGAGGA AGGTCCTTCC TGCCCGGGCC AGCCTGACAC CTACACATCC 
GTTTGGTTGG GAGACTCCCT AGGATTGCCA ACTCAAAAGA ATGATCTGTC TTCGTCCTCG 
GGGTCACTGA ACCTGTCCCA TGGCTCCAGT TCCCTAGAGC ACAGATCTGT GGACAGCAGT 
CTGTGTGACC TGCTGAGGGA CCCCAGTGCC TCCCCAAGGA GCAGACCACA GGGCTCTAGG 
CGAGCAAGAG CCAGTTCAGC GCTGGTGGTA TGTGGGGGCC AGGGCCACCG GCGGGTGCAC 
AGGAAGGCCA GGCAGCCTTC TCAGGAGGAC CTGGTTTCTT CAGTGATGGT GTGGCAGATC 
CCCCTGCTGG GCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099286.1
CDS	236..4273
Translation	MEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPAPSSPGSPWAEFCLICVFLPCLPSGAYAISGTGCGAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_030243426.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243426.1

ORF Insert Sequence:

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAAATGC AGAAGCTTAT GAAGGCAGCC AAGGAGGGCA CCAAAGATGG GCTGGAGAAA 
ACCAAAGCTG CTGTGAAGAG AGGCGGCTCC TTCATCAGGA CCAGGTCCTT GGTCTCTCAA 
GATCACAGGT GTTATTTTGA AGAAGAGCAG AATTTGTTCA TTGACGTGGA CTGCAAGCAC 
CCGGAGGCTG TCCTCACCCC AATGCCCGAG GGCTTGTCTC AGCAGCAGGT TGTGAGAAGA 
TACATTCTGG GCTCCATTGT TGAGAGCGAG AAGAACTATG TAGATGCTCT CCGGAGGATT 
TTGGAGCAAT ATGAGAAGCC GCTGTCTGAG ATGGAGCCCC GGCTCCTGAG TGACAGGAAG 
CTCAGGATGG TTTTCTACCG CGTCAAGGAG ATCCTGCAGT GTCACTCCAT GTTCCAGATT 
GCGCTGGCCA GCCGCGTGTC CGAGTGGGAT GTCGTGGAAA CAATCGGCGA CGTTTTTGTG 
GCCTCGTTCT CCAAGTCCAT GGTGCTGGAT GCTTACAGCG AGTACGTGAA CAACTTCAGC 
ACGGCCGTGG CTGTCCTCAA GAAGACGTGT GCCACAAAGC CTGCTTTCCT TGAGTTCTTA 
AAGCTGAGCC AGGATTCCAG TCCAGACCGA GTCACACTCC ACAGCCTTAT GATGCGGCCC 
ATCCAGAGGT TCCCACAGTT CATCCTCCTA CTGCAGGACA TGCTGAAGAA CACCGCCAAA 
GGACATCCTG ACAGGCTGCC CCTTCAGATG GCGCTGACGG AGCTCGAGAC CCTGGCAGAG 
AAGCTAAACG AGAGAAAAAG AGATGCCGAC CAGCGATGCG AAATCAAACA AATAGCAAAA 
GCCATAAACG AGAGATACCT GAACAAGCTC CTCAGCAGTG GAAACCGGTA CCTCGTCCGG 
TCCGATGACG TCATCGAGAC GGTTTACAAC GACCGGGGAG AAATCGTCAA GACCAAACAA 
CGCCGGATCT TCATGCTGAA TGACGTGCTC ATGTGTGCCA CCGCCAGCTC ACGGAACTCA 
CATGAAAGCC ATGCCGTGAT GAGCCAGAGG TACCTGCTCA AGTGGAGTGT CCCTCTAGGT 
CACGTGGATG TGATTCAGTA CAATGGAGGC TCTGGCGCCG GGGAGCACTG TCGGCACCAC 
GCTGCACACT CACCTGAGAG CCTGGCCGTG GTGGCCAACG CGAAGCCACA CAAAGTTTAC 
ATGGGGCCAG GGCAGCTGTA CCAGGATCTG CAGAACCTAC TGCACGACCT GAACGTTGTT 
GGCCAGATCT CGCAGCTGAT AGGAAACCTC CGAGGGAGCT ATCAGAACTT AAACCAGTCT 
GTAGCCCATG ATTGGACGTC GGGCTTACAG CGGCTGATAC TGAGGAAAGA AGATGCGATC 
AGAGCTGCCG ACCGCTGCCG GATCCAGTTA CAGCTCCCGG GAAAGCAGGA CAAGTCCGGG 
CGGCCCACGT TCTTCACTGC TGTGTTCAAT ACGCTTACAC CAGCCATCAA GGAGTCCTGG 
GTCAGCAGCC TGCAGATGGC CAAGCTGGCC CTTGAGGAGG AGAACCATAT GGGATGGTTC 
TGTGTGGATG ACGATGGCAA CCTAGCCAAG AAGGAGACAC ACCCTCTCCT GGTGGGACAC 
ATGCCTGTGA TGGTGGCCAA GCAGCCAGAG TTCAAGATTG AATGTGCAGC TTATAACCCT 
GAACCTTACC TGAGCAATGA AAGTCAACCA GCCTCACCCT CCACAGCCCG TGGCTTTCTG 
TGGATTGGAA GCTGTAGCAA CCAGATGGGT CAGGTCGCCA TTGTCTCCTT CCAAGGTTCC 
AACCCCAAAG TCATCGAGTG CTTCAATGTG GAATCACGCA TCCTGTGTAT GGTATACATT 
CCCGCAGAGG AGTCAAAGCC CCAAGAAACC ACCGAAACCA AGGATCCAGA AGCCACAGCC 
TCGAGAGCAC CCCATGTGCC CACCATCTGC CTGGGCACAG AAGAGGGAAG CATTTCCATT 
TACAAAAGCA GTCAGGGCTG CAAGAAGGTG AGGCTTCAGC ACTTCTATGC ACCGGACAAG 
TCCACGGTCA TGAGCTTGGC CTGCTCCCCA CAAGGCCTGT ATGCAGGCCT GGTCAATGGG 
TCCGTAGCCA GCTACACCAA GGCTCCAGCA CCGAGCTCTC CTGGCAGCCC GTGGGCAGAG 
TTCTGTCTCA TCTGCGTTTT CCTGCCATGC TTGCCCTCGG GAGCATATGC CATCTCGGGT 
ACAGGCTGTG GTGCAGTGAA GGGCTATGGA TCCTGGAACT CGGAACCCCA GCAAGTGATC 
AAGTTGGGTG TCCTGCCAGT CAGAAGCCTT CTCCTGGTGG AGGGTGCACT GTGGGCAGCC 
TCGGGAGGGC AGGTGTTCAT GGCCAGCGTG GAGACTCACA CTATAGAGAA TCAGCTGGAG 
GCCCACCAAG ATGAAGGGAT GGTCATCTCC CACATGGCTG TGGCTGGCGT TGGCATCTGG 
ATTGCCTTCA CCTCGGGGTC CACCCTCCGC CTCTTCCACA CAGAGACCTT GAAGCACCTG 
CAGGATGTCA ACATTGATGC CCCTGTGCAT AGCATGCTGC CAGGGCACCA GCGTCTATCT 
GTGACCAGCC TGCTCGTCTG CCACGGCTTG CTCATGGTCG GTACCAGCCT GGGTGTCGTG 
GTGGCCCTGC CAGTCCCTCG GCTTCAGGGT ATCCCCAAAG TGACAGGGCG AGGCATGGTC 
TCCTACCATG CGCATAACGG GCCCGTTAAG TTCATCGTCT CAGCCACGGC GTTTCAAAAC 
AAGGACAGAG CCAGGGACAG CCCACGTTCC GGTTCTGAGC TTCAGGATGA AGACCCCAAG 
GACTTGCTCT GCAGCGAGGA AGGTCCTTCC TGCCCGGGCC AGCCTGACAC CTACACATCC 
GTTTGGTTGG GAGACTCCCT AGGATTGCCA ACTCAAAAGA ATGATCTGTC TTCGTCCTCG 
GGGTCACTGA ACCTGTCCCA TGGCTCCAGT TCCCTAGAGC ACAGATCTGT GGACAGCAGT 
CTGTGTGACC TGCTGAGGGA CCCCAGTGCC TCCCCAAGGA GCAGACCACA GGGCTCTAGG 
CGAGCAAGAG CCAGTTCAGC GCTGGTGGTA TGTGGGGGCC AGGGCCACCG GCGGGTGCAC 
AGGAAGGCCA GGCAGCCTTC TCAGGAGGAC CTGGTTTCTT CAGTGATGGT GTGGCAGATC 
CCCCTGCTGG GCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_017312668.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168157.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X2, mRNA.

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CloneID	OMu82406
Clone ID Related Accession (Same CDS sequence)	XM_017312668.1
Accession Version	XM_017312668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCG GTCCCTCGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG TGAGCAAGGC AGCCAAGAAG GCAGTGCCCT GGCCCGATGG 
GCAGCCGACC CTGCCAACAC AGCATGGATG GAAAATCCAG AGGAAGCAAT TTACGACGAC 
GTTCCGAGGG AAAACTCGGA CTCTGAGCCA GATGAAATGA TTTATGATGA TGTGGAGAAC 
GGCGAAGAAG GTGGAAACAG CTCCCCAGAG TACGGGTGGA GTTCCAGCGA ATTCGAAAGC 
TATGAGGAGC CAAGCGACTC GGAGGGCAAG AACGGAATTC CCAGGTCCTT CCTGCGCAGC 
AGTCATAAAA AGCAACTCTC TCATGACCTA ACCCGCTTTA AGGCGCACTG TGAGGAAAAG 
ATGAGAGGCT TGGTGGCCAG TACGGTGGGA GCCATGGAGA TTCAGCAGGC AAAGCAGAGG 
CAGGAGCGGA AGATGCAGAA GCTTATGAAG GCAGCCAAGG AGGGCACCAA AGATGGGCTG 
GAGAAAACCA AAGCTGCTGT GAAGAGAGGC GGCTCCTTCA TCAGGACCAG GTCCTTGGTC 
TCTCAAGATC ACAGGTGTTA TTTTGAAGAA GAGCAGAATT TGTTCATTGA CGTGGACTGC 
AAGCACCCGG AGGCTGTCCT CACCCCAATG CCCGAGGGCT TGTCTCAGCA GCAGGTTGTG 
AGAAGATACA TTCTGGGCTC CATTGTTGAG AGCGAGAAGA ACTATGTAGA TGCTCTCCGG 
AGGATTTTGG AGCAATATGA GAAGCCGCTG TCTGAGATGG AGCCCCGGCT CCTGAGTGAC 
AGGAAGCTCA GGATGGTTTT CTACCGCGTC AAGGAGATCC TGCAGTGTCA CTCCATGTTC 
CAGATTGCGC TGGCCAGCCG CGTGTCCGAG TGGGATGTCG TGGAAACAAT CGGCGACGTT 
TTTGTGGCCT CGTTCTCCAA GTCCATGGTG CTGGATGCTT ACAGCGAGTA CGTGAACAAC 
TTCAGCACGG CCGTGGCTGT CCTCAAGAAG ACGTGTGCCA CAAAGCCTGC TTTCCTTGAG 
TTCTTAAAGC TGAGCCAGGA TTCCAGTCCA GACCGAGTCA CACTCCACAG CCTTATGATG 
CGGCCCATCC AGAGGTTCCC ACAGTTCATC CTCCTACTGC AGGACATGCT GAAGAACACC 
GCCAAAGGAC ATCCTGACAG GCTGCCCCTT CAGATGGCGC TGACGGAGCT CGAGACCCTG 
GCAGAGAAGC TAAACGAGAG AAAAAGAGAT GCCGACCAGC GATGCGAAAT CAAACAAATA 
GCAAAAGCCA TAAACGAGAG ATACCTGAAC AAGCTCCTCA GCAGTGGAAA CCGGTACCTC 
GTCCGGTCCG ATGACGTCAT CGAGACGGTT TACAACGACC GGGGAGAAAT CGTCAAGACC 
AAACAACGCC GGATCTTCAT GCTGAATGAC GTGCTCATGT GTGCCACCGC CAGCTCACGG 
AACTCACATG AAAGCCATGC CGTGATGAGC CAGAGGTACC TGCTCAAGTG GAGTGTCCCT 
CTAGGTCACG TGGATGTGAT TCAGTACAAT GGAGGCTCTG GCGCCGGGGA GCACTGTCGG 
CACCACGCTG CACACTCACC TGAGAGCCTG GCCGTGGTGG CCAACGCGAA GCCACACAAA 
GTTTACATGG GGCCAGGGCA GCTGTACCAG GATCTGCAGA ACCTACTGCA CGACCTGAAC 
GTTGTTGGCC AGATCTCGCA GCTGATAGGA AACCTCCGAG GGAGCTATCA GAACTTAAAC 
CAGTCTGTAG CCCATGATTG GACGTCGGGC TTACAGCGGC TGATACTGAG GAAAGAAGAT 
GCGATCAGAG CTGCCGACCG CTGCCGGATC CAGTTACAGC TCCCGGGAAA GCAGGACAAG 
TCCGGGCGGC CCACGTTCTT CACTGCTGTG TTCAATACGC TTACACCAGC CATCAAGGAG 
TCCTGGGTCA GCAGCCTGCA GATGGCCAAG CTGGCCCTTG AGGAGGAGAA CCATATGGGA 
TGGTTCTGTG TGGATGACGA TGGCAACCTA GCCAAGAAGG AGACACACCC TCTCCTGGTG 
GGACACATGC CTGTGATGGT GGCCAAGCAG CCAGAGTTCA AGATTGAATG TGCAGCTTAT 
AACCCTGAAC CTTACCTGAG CAATGAAAGT CAACCAGCCT CACCCTCCAC AGCCCGTGGC 
TTTCTGTGGA TTGGAAGCTG TAGCAACCAG ATGGGTCAGG TCGCCATTGT CTCCTTCCAA 
GGTTCCAACC CCAAAGTCAT CGAGTGCTTC AATGTGGAAT CACGCATCCT GTGTATGGTA 
TACATTCCCG CAGAGGAGTC AAAGCCCCAA GAAACCACCG AAACCAAGGA TCCAGAAGCC 
ACAGCCTCGA GAGCACCCCA TGTGCCCACC ATCTGCCTGG GCACAGAAGA GGGAAGCATT 
TCCATTTACA AAAGCAGTCA GGGCTGCAAG AAGGTGAGGC TTCAGCACTT CTATGCACCG 
GACAAGTCCA CGGTCATGAG CTTGGCCTGC TCCCCACAAG GCCTGTATGC AGGCCTGGTC 
AATGGGTCCG TAGCCAGCTA CACCAAGGCT CCAGCACCGA GCTCTCCTGG CAGCCCGTGG 
GCAGAGTTCT GTCTCATCTG CGTTTTCCTG CCATGCTTGC CCTCGGGAGC ATATGCCATC 
TCGGGTACAG GCTGTGGTGC AGTGAAGGGC TATGGATCCT GGAACTCGGA ACCCCAGCAA 
GTGATCAAGT TGGGTGTCCT GCCAGTCAGA AGCCTTCTCC TGGTGGAGGG TGCACTGTGG 
GCAGCCTCGG GAGGGCAGGT GTTCATGGCC AGCGTGGAGA CTCACACTAT AGAGAATCAG 
CTGGAGGCCC ACCAAGATGA AGGGATGGTC ATCTCCCACA TGGCTGTGGC TGGCGTTGGC 
ATCTGGATTG CCTTCACCTC GGGGTCCACC CTCCGCCTCT TCCACACAGA GACCTTGAAG 
CACCTGCAGG ATGTCAACAT TGATGCCCCT GTGCATAGCA TGCTGCCAGG GCACCAGCGT 
CTATCTGTGA CCAGCCTGCT CGTCTGCCAC GGCTTGCTCA TGGTCGGTAC CAGCCTGGGT 
GTCGTGGTGG CCCTGCCAGT CCCTCGGCTT CAGGGTATCC CCAAAGTGAC AGGGCGAGGC 
ATGGTCTCCT ACCATGCGCA TAACGGGCCC GTTAAGTTCA TCGTCTCAGC CACGGCGTTT 
CAAAACAAGG ACAGAGCCAG GGACAGCCCA CGTTCCGGTT CTGAGCTTCA GGATGAAGAC 
CCCAAGGACT TGCTCTGCAG CGAGGAAGGT CCTTCCTGCC CGGGCCAGCC TGACACCTAC 
ACATCCGTTT GGTTGGGAGA CTCCCTAGGA TTGCCAACTC AAAAGAATGA TCTGTCTTCG 
TCCTCGGGGT CACTGAACCT GTCCCATGGC TCCAGTTCCC TAGAGCACAG ATCTGTGGAC 
AGCAGTCTGT GTGACCTGCT GAGGGACCCC AGTGCCTCCC CAAGGAGCAG ACCACAGGGC 
TCTAGGCGAG CAAGAGCCAG TTCAGCGCTG GTGGTATGTG GGGGCCAGGG CCACCGGCGG 
GTGCACAGGA AGGCCAGGCA GCCTTCTCAG GAGGACCTGG TTTCTTCAGT GATGGTGTGG 
CAGATCCCCC TGCTGGGCAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168157.1
CDS	78..4301
Translation	MAPGPSGPLSPDAVQAWRKEARLSMEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQLSHDLTRFKAHCEEKMRGLVASTVGAMEIQQAKQRQERKMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPAPSSPGSPWAEFCLICVFLPCLPSGAYAISGTGCGAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_017312668.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312668.1

ORF Insert Sequence:

ATGGCGCCCG GTCCCTCGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG TGAGCAAGGC AGCCAAGAAG GCAGTGCCCT GGCCCGATGG 
GCAGCCGACC CTGCCAACAC AGCATGGATG GAAAATCCAG AGGAAGCAAT TTACGACGAC 
GTTCCGAGGG AAAACTCGGA CTCTGAGCCA GATGAAATGA TTTATGATGA TGTGGAGAAC 
GGCGAAGAAG GTGGAAACAG CTCCCCAGAG TACGGGTGGA GTTCCAGCGA ATTCGAAAGC 
TATGAGGAGC CAAGCGACTC GGAGGGCAAG AACGGAATTC CCAGGTCCTT CCTGCGCAGC 
AGTCATAAAA AGCAACTCTC TCATGACCTA ACCCGCTTTA AGGCGCACTG TGAGGAAAAG 
ATGAGAGGCT TGGTGGCCAG TACGGTGGGA GCCATGGAGA TTCAGCAGGC AAAGCAGAGG 
CAGGAGCGGA AGATGCAGAA GCTTATGAAG GCAGCCAAGG AGGGCACCAA AGATGGGCTG 
GAGAAAACCA AAGCTGCTGT GAAGAGAGGC GGCTCCTTCA TCAGGACCAG GTCCTTGGTC 
TCTCAAGATC ACAGGTGTTA TTTTGAAGAA GAGCAGAATT TGTTCATTGA CGTGGACTGC 
AAGCACCCGG AGGCTGTCCT CACCCCAATG CCCGAGGGCT TGTCTCAGCA GCAGGTTGTG 
AGAAGATACA TTCTGGGCTC CATTGTTGAG AGCGAGAAGA ACTATGTAGA TGCTCTCCGG 
AGGATTTTGG AGCAATATGA GAAGCCGCTG TCTGAGATGG AGCCCCGGCT CCTGAGTGAC 
AGGAAGCTCA GGATGGTTTT CTACCGCGTC AAGGAGATCC TGCAGTGTCA CTCCATGTTC 
CAGATTGCGC TGGCCAGCCG CGTGTCCGAG TGGGATGTCG TGGAAACAAT CGGCGACGTT 
TTTGTGGCCT CGTTCTCCAA GTCCATGGTG CTGGATGCTT ACAGCGAGTA CGTGAACAAC 
TTCAGCACGG CCGTGGCTGT CCTCAAGAAG ACGTGTGCCA CAAAGCCTGC TTTCCTTGAG 
TTCTTAAAGC TGAGCCAGGA TTCCAGTCCA GACCGAGTCA CACTCCACAG CCTTATGATG 
CGGCCCATCC AGAGGTTCCC ACAGTTCATC CTCCTACTGC AGGACATGCT GAAGAACACC 
GCCAAAGGAC ATCCTGACAG GCTGCCCCTT CAGATGGCGC TGACGGAGCT CGAGACCCTG 
GCAGAGAAGC TAAACGAGAG AAAAAGAGAT GCCGACCAGC GATGCGAAAT CAAACAAATA 
GCAAAAGCCA TAAACGAGAG ATACCTGAAC AAGCTCCTCA GCAGTGGAAA CCGGTACCTC 
GTCCGGTCCG ATGACGTCAT CGAGACGGTT TACAACGACC GGGGAGAAAT CGTCAAGACC 
AAACAACGCC GGATCTTCAT GCTGAATGAC GTGCTCATGT GTGCCACCGC CAGCTCACGG 
AACTCACATG AAAGCCATGC CGTGATGAGC CAGAGGTACC TGCTCAAGTG GAGTGTCCCT 
CTAGGTCACG TGGATGTGAT TCAGTACAAT GGAGGCTCTG GCGCCGGGGA GCACTGTCGG 
CACCACGCTG CACACTCACC TGAGAGCCTG GCCGTGGTGG CCAACGCGAA GCCACACAAA 
GTTTACATGG GGCCAGGGCA GCTGTACCAG GATCTGCAGA ACCTACTGCA CGACCTGAAC 
GTTGTTGGCC AGATCTCGCA GCTGATAGGA AACCTCCGAG GGAGCTATCA GAACTTAAAC 
CAGTCTGTAG CCCATGATTG GACGTCGGGC TTACAGCGGC TGATACTGAG GAAAGAAGAT 
GCGATCAGAG CTGCCGACCG CTGCCGGATC CAGTTACAGC TCCCGGGAAA GCAGGACAAG 
TCCGGGCGGC CCACGTTCTT CACTGCTGTG TTCAATACGC TTACACCAGC CATCAAGGAG 
TCCTGGGTCA GCAGCCTGCA GATGGCCAAG CTGGCCCTTG AGGAGGAGAA CCATATGGGA 
TGGTTCTGTG TGGATGACGA TGGCAACCTA GCCAAGAAGG AGACACACCC TCTCCTGGTG 
GGACACATGC CTGTGATGGT GGCCAAGCAG CCAGAGTTCA AGATTGAATG TGCAGCTTAT 
AACCCTGAAC CTTACCTGAG CAATGAAAGT CAACCAGCCT CACCCTCCAC AGCCCGTGGC 
TTTCTGTGGA TTGGAAGCTG TAGCAACCAG ATGGGTCAGG TCGCCATTGT CTCCTTCCAA 
GGTTCCAACC CCAAAGTCAT CGAGTGCTTC AATGTGGAAT CACGCATCCT GTGTATGGTA 
TACATTCCCG CAGAGGAGTC AAAGCCCCAA GAAACCACCG AAACCAAGGA TCCAGAAGCC 
ACAGCCTCGA GAGCACCCCA TGTGCCCACC ATCTGCCTGG GCACAGAAGA GGGAAGCATT 
TCCATTTACA AAAGCAGTCA GGGCTGCAAG AAGGTGAGGC TTCAGCACTT CTATGCACCG 
GACAAGTCCA CGGTCATGAG CTTGGCCTGC TCCCCACAAG GCCTGTATGC AGGCCTGGTC 
AATGGGTCCG TAGCCAGCTA CACCAAGGCT CCAGCACCGA GCTCTCCTGG CAGCCCGTGG 
GCAGAGTTCT GTCTCATCTG CGTTTTCCTG CCATGCTTGC CCTCGGGAGC ATATGCCATC 
TCGGGTACAG GCTGTGGTGC AGTGAAGGGC TATGGATCCT GGAACTCGGA ACCCCAGCAA 
GTGATCAAGT TGGGTGTCCT GCCAGTCAGA AGCCTTCTCC TGGTGGAGGG TGCACTGTGG 
GCAGCCTCGG GAGGGCAGGT GTTCATGGCC AGCGTGGAGA CTCACACTAT AGAGAATCAG 
CTGGAGGCCC ACCAAGATGA AGGGATGGTC ATCTCCCACA TGGCTGTGGC TGGCGTTGGC 
ATCTGGATTG CCTTCACCTC GGGGTCCACC CTCCGCCTCT TCCACACAGA GACCTTGAAG 
CACCTGCAGG ATGTCAACAT TGATGCCCCT GTGCATAGCA TGCTGCCAGG GCACCAGCGT 
CTATCTGTGA CCAGCCTGCT CGTCTGCCAC GGCTTGCTCA TGGTCGGTAC CAGCCTGGGT 
GTCGTGGTGG CCCTGCCAGT CCCTCGGCTT CAGGGTATCC CCAAAGTGAC AGGGCGAGGC 
ATGGTCTCCT ACCATGCGCA TAACGGGCCC GTTAAGTTCA TCGTCTCAGC CACGGCGTTT 
CAAAACAAGG ACAGAGCCAG GGACAGCCCA CGTTCCGGTT CTGAGCTTCA GGATGAAGAC 
CCCAAGGACT TGCTCTGCAG CGAGGAAGGT CCTTCCTGCC CGGGCCAGCC TGACACCTAC 
ACATCCGTTT GGTTGGGAGA CTCCCTAGGA TTGCCAACTC AAAAGAATGA TCTGTCTTCG 
TCCTCGGGGT CACTGAACCT GTCCCATGGC TCCAGTTCCC TAGAGCACAG ATCTGTGGAC 
AGCAGTCTGT GTGACCTGCT GAGGGACCCC AGTGCCTCCC CAAGGAGCAG ACCACAGGGC 
TCTAGGCGAG CAAGAGCCAG TTCAGCGCTG GTGGTATGTG GGGGCCAGGG CCACCGGCGG 
GTGCACAGGA AGGCCAGGCA GCCTTCTCAG GAGGACCTGG TTTCTTCAGT GATGGTGTGG 
CAGATCCCCC TGCTGGGCAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_017312669.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168158.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu82407
Clone ID Related Accession (Same CDS sequence)	XM_017312669.2 , XM_017312669.1
Accession Version	XM_017312669.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312669.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGAG TGTCTGTCTT GTGTTCCCTG CCAGCGACAG CGCAGCTCCC AGTGTCTAAC 
AGAGGGCGGA TGATCGGGAG ACACAATCTG GCCCCGCGCC ATTTCCCAGT GACCAGGAGT 
CTGAGGGGAG AAGCGTCCAC TCTGGGGCAC TACAAGCTGG TGTCCTTTCT GTGCCCCCTC 
TGTCCCCGGG GGCGCAAGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG CAGTGAGCAA GGCAGCCAAG AAGGCAGTGC CCTGGCCCGA 
TGGGCAGCCG ACCCTGCCAA CACAGCATGG ATGGAAAATC CAGAGGAAGC AATTTACGAC 
GACGTTCCGA GGGAAAACTC GGACTCTGAG CCAGATGAAA TGATTTATGA TGATGTGGAG 
AACGGCGAAG AAGGTGGAAA CAGCTCCCCA GAGTACGGGT GGAGTTCCAG CGAATTCGAA 
AGCTATGAGG AGCCAAGCGA CTCGGAGGGC AAGAACGGAA TTCCCAGGTC CTTCCTGCGC 
AGCAGTCATA AAAAGCAACT CTCTCATGAC CTAACCCGCT TTAAGGCGCA CTGTGAGGAA 
AAGATGAGAG GCTTGGTGGC CAGTACGGTG GGAGCCATGG AGATTCAGCA GGCAAAGCAG 
AGGCAGGAGC GGAAGATGCA GAAGCTTATG AAGGCAGCCA AGGAGGGCAC CAAAGATGGG 
CTGGAGAAAA CCAAAGCTGC TGTGAAGAGA GGCGGCTCCT TCATCAGGAC CAGGTCCTTG 
GTCTCTCAAG ATCACAGGTG TTATTTTGAA GAAGAGCAGA ATTTGTTCAT TGACGTGGAC 
TGCAAGCACC CGGAGGCTGT CCTCACCCCA ATGCCCGAGG GCTTGTCTCA GCAGCAGGTT 
GTGAGAAGAT ACATTCTGGG CTCCATTGTT GAGAGCGAGA AGAACTATGT AGATGCTCTC 
CGGAGGATTT TGGAGCAATA TGAGAAGCCG CTGTCTGAGA TGGAGCCCCG GCTCCTGAGT 
GACAGGAAGC TCAGGATGGT TTTCTACCGC GTCAAGGAGA TCCTGCAGTG TCACTCCATG 
TTCCAGATTG CGCTGGCCAG CCGCGTGTCC GAGTGGGATG TCGTGGAAAC AATCGGCGAC 
GTTTTTGTGG CCTCGTTCTC CAAGTCCATG GTGCTGGATG CTTACAGCGA GTACGTGAAC 
AACTTCAGCA CGGCCGTGGC TGTCCTCAAG AAGACGTGTG CCACAAAGCC TGCTTTCCTT 
GAGTTCTTAA AGCTGAGCCA GGATTCCAGT CCAGACCGAG TCACACTCCA CAGCCTTATG 
ATGCGGCCCA TCCAGAGGTT CCCACAGTTC ATCCTCCTAC TGCAGGACAT GCTGAAGAAC 
ACCGCCAAAG GACATCCTGA CAGGCTGCCC CTTCAGATGG CGCTGACGGA GCTCGAGACC 
CTGGCAGAGA AGCTAAACGA GAGAAAAAGA GATGCCGACC AGCGATGCGA AATCAAACAA 
ATAGCAAAAG CCATAAACGA GAGATACCTG AACAAGCTCC TCAGCAGTGG AAACCGGTAC 
CTCGTCCGGT CCGATGACGT CATCGAGACG GTTTACAACG ACCGGGGAGA AATCGTCAAG 
ACCAAACAAC GCCGGATCTT CATGCTGAAT GACGTGCTCA TGTGTGCCAC CGCCAGCTCA 
CGGAACTCAC ATGAAAGCCA TGCCGTGATG AGCCAGAGGT ACCTGCTCAA GTGGAGTGTC 
CCTCTAGGTC ACGTGGATGT GATTCAGTAC AATGGAGGCT CTGGCGCCGG GGAGCACTGT 
CGGCACCACG CTGCACACTC ACCTGAGAGC CTGGCCGTGG TGGCCAACGC GAAGCCACAC 
AAAGTTTACA TGGGGCCAGG GCAGCTGTAC CAGGATCTGC AGAACCTACT GCACGACCTG 
AACGTTGTTG GCCAGATCTC GCAGCTGATA GGAAACCTCC GAGGGAGCTA TCAGAACTTA 
AACCAGTCTG TAGCCCATGA TTGGACGTCG GGCTTACAGC GGCTGATACT GAGGAAAGAA 
GATGCGATCA GAGCTGCCGA CCGCTGCCGG ATCCAGTTAC AGCTCCCGGG AAAGCAGGAC 
AAGTCCGGGC GGCCCACGTT CTTCACTGCT GTGTTCAATA CGCTTACACC AGCCATCAAG 
GAGTCCTGGG TCAGCAGCCT GCAGATGGCC AAGCTGGCCC TTGAGGAGGA GAACCATATG 
GGATGGTTCT GTGTGGATGA CGATGGCAAC CTAGCCAAGA AGGAGACACA CCCTCTCCTG 
GTGGGACACA TGCCTGTGAT GGTGGCCAAG CAGCCAGAGT TCAAGATTGA ATGTGCAGCT 
TATAACCCTG AACCTTACCT GAGCAATGAA AGTCAACCAG CCTCACCCTC CACAGCCCGT 
GGCTTTCTGT GGATTGGAAG CTGTAGCAAC CAGATGGGTC AGGTCGCCAT TGTCTCCTTC 
CAAGGTTCCA ACCCCAAAGT CATCGAGTGC TTCAATGTGG AATCACGCAT CCTGTGTATG 
GTATACATTC CCGCAGAGGA GTCAAAGCCC CAAGAAACCA CCGAAACCAA GGATCCAGAA 
GCCACAGCCT CGAGAGCACC CCATGTGCCC ACCATCTGCC TGGGCACAGA AGAGGGAAGC 
ATTTCCATTT ACAAAAGCAG TCAGGGCTGC AAGAAGGTGA GGCTTCAGCA CTTCTATGCA 
CCGGACAAGT CCACGGTCAT GAGCTTGGCC TGCTCCCCAC AAGGCCTGTA TGCAGGCCTG 
GTCAATGGGT CCGTAGCCAG CTACACCAAG GCTCCAGCAC CGAGCTCTCC TGGCAGCCCG 
TGGGCAGAGT TCTGTCTCAT CTGCGTTTTC CTGCCATGCT TGCCCTCGGG AGCATATGCC 
ATCTCGGGTA CAGGCTGTGG TGCAGTGAAG GGCTATGGAT CCTGGAACTC GGAACCCCAG 
CAAGTGATCA AGTTGGGTGT CCTGCCAGTC AGAAGCCTTC TCCTGGTGGA GGGTGCACTG 
TGGGCAGCCT CGGGAGGGCA GGTGTTCATG GCCAGCGTGG AGACTCACAC TATAGAGAAT 
CAGCTGGAGG CCCACCAAGA TGAAGGGATG GTCATCTCCC ACATGGCTGT GGCTGGCGTT 
GGCATCTGGA TTGCCTTCAC CTCGGGGTCC ACCCTCCGCC TCTTCCACAC AGAGACCTTG 
AAGCACCTGC AGGATGTCAA CATTGATGCC CCTGTGCATA GCATGCTGCC AGGGCACCAG 
CGTCTATCTG TGACCAGCCT GCTCGTCTGC CACGGCTTGC TCATGGTCGG TACCAGCCTG 
GGTGTCGTGG TGGCCCTGCC AGTCCCTCGG CTTCAGGGTA TCCCCAAAGT GACAGGGCGA 
GGCATGGTCT CCTACCATGC GCATAACGGG CCCGTTAAGT TCATCGTCTC AGCCACGGCG 
TTTCAAAACA AGGACAGAGC CAGGGACAGC CCACGTTCCG GTTCTGAGCT TCAGGATGAA 
GACCCCAAGG ACTTGCTCTG CAGCGAGGAA GGTCCTTCCT GCCCGGGCCA GCCTGACACC 
TACACATCCG TTTGGTTGGG AGACTCCCTA GGATTGCCAA CTCAAAAGAA TGATCTGTCT 
TCGTCCTCGG GGTCACTGAA CCTGTCCCAT GGCTCCAGTT CCCTAGAGCA CAGATCTGTG 
GACAGCAGTC TGTGTGACCT GCTGAGGGAC CCCAGTGCCT CCCCAAGGAG CAGACCACAG 
GGCTCTAGGC GAGCAAGAGC CAGTTCAGCG CTGGTGGTAT GTGGGGGCCA GGGCCACCGG 
CGGGTGCACA GGAAGGCCAG GCAGCCTTCT CAGGAGGACC TGGTTTCTTC AGTGATGGTG 
TGGCAGATCC CCCTGCTGGG CATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168158.1
CDS	2773..7179
Translation	MGGVSVLCSLPATAQLPVSNRGRMIGRHNLAPRHFPVTRSLRGEASTLGHYKLVSFLCPLCPRGRKGPLSPDAVQAWRKEARLSMEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQLSHDLTRFKAHCEEKMRGLVASTVGAMEIQQAKQRQERKMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPAPSSPGSPWAEFCLICVFLPCLPSGAYAISGTGCGAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_017312669.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312669.2

ORF Insert Sequence:

ATGGGTGGAG TGTCTGTCTT GTGTTCCCTG CCAGCGACAG CGCAGCTCCC AGTGTCTAAC 
AGAGGGCGGA TGATCGGGAG ACACAATCTG GCCCCGCGCC ATTTCCCAGT GACCAGGAGT 
CTGAGGGGAG AAGCGTCCAC TCTGGGGCAC TACAAGCTGG TGTCCTTTCT GTGCCCCCTC 
TGTCCCCGGG GGCGCAAGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG CAGTGAGCAA GGCAGCCAAG AAGGCAGTGC CCTGGCCCGA 
TGGGCAGCCG ACCCTGCCAA CACAGCATGG ATGGAAAATC CAGAGGAAGC AATTTACGAC 
GACGTTCCGA GGGAAAACTC GGACTCTGAG CCAGATGAAA TGATTTATGA TGATGTGGAG 
AACGGCGAAG AAGGTGGAAA CAGCTCCCCA GAGTACGGGT GGAGTTCCAG CGAATTCGAA 
AGCTATGAGG AGCCAAGCGA CTCGGAGGGC AAGAACGGAA TTCCCAGGTC CTTCCTGCGC 
AGCAGTCATA AAAAGCAACT CTCTCATGAC CTAACCCGCT TTAAGGCGCA CTGTGAGGAA 
AAGATGAGAG GCTTGGTGGC CAGTACGGTG GGAGCCATGG AGATTCAGCA GGCAAAGCAG 
AGGCAGGAGC GGAAGATGCA GAAGCTTATG AAGGCAGCCA AGGAGGGCAC CAAAGATGGG 
CTGGAGAAAA CCAAAGCTGC TGTGAAGAGA GGCGGCTCCT TCATCAGGAC CAGGTCCTTG 
GTCTCTCAAG ATCACAGGTG TTATTTTGAA GAAGAGCAGA ATTTGTTCAT TGACGTGGAC 
TGCAAGCACC CGGAGGCTGT CCTCACCCCA ATGCCCGAGG GCTTGTCTCA GCAGCAGGTT 
GTGAGAAGAT ACATTCTGGG CTCCATTGTT GAGAGCGAGA AGAACTATGT AGATGCTCTC 
CGGAGGATTT TGGAGCAATA TGAGAAGCCG CTGTCTGAGA TGGAGCCCCG GCTCCTGAGT 
GACAGGAAGC TCAGGATGGT TTTCTACCGC GTCAAGGAGA TCCTGCAGTG TCACTCCATG 
TTCCAGATTG CGCTGGCCAG CCGCGTGTCC GAGTGGGATG TCGTGGAAAC AATCGGCGAC 
GTTTTTGTGG CCTCGTTCTC CAAGTCCATG GTGCTGGATG CTTACAGCGA GTACGTGAAC 
AACTTCAGCA CGGCCGTGGC TGTCCTCAAG AAGACGTGTG CCACAAAGCC TGCTTTCCTT 
GAGTTCTTAA AGCTGAGCCA GGATTCCAGT CCAGACCGAG TCACACTCCA CAGCCTTATG 
ATGCGGCCCA TCCAGAGGTT CCCACAGTTC ATCCTCCTAC TGCAGGACAT GCTGAAGAAC 
ACCGCCAAAG GACATCCTGA CAGGCTGCCC CTTCAGATGG CGCTGACGGA GCTCGAGACC 
CTGGCAGAGA AGCTAAACGA GAGAAAAAGA GATGCCGACC AGCGATGCGA AATCAAACAA 
ATAGCAAAAG CCATAAACGA GAGATACCTG AACAAGCTCC TCAGCAGTGG AAACCGGTAC 
CTCGTCCGGT CCGATGACGT CATCGAGACG GTTTACAACG ACCGGGGAGA AATCGTCAAG 
ACCAAACAAC GCCGGATCTT CATGCTGAAT GACGTGCTCA TGTGTGCCAC CGCCAGCTCA 
CGGAACTCAC ATGAAAGCCA TGCCGTGATG AGCCAGAGGT ACCTGCTCAA GTGGAGTGTC 
CCTCTAGGTC ACGTGGATGT GATTCAGTAC AATGGAGGCT CTGGCGCCGG GGAGCACTGT 
CGGCACCACG CTGCACACTC ACCTGAGAGC CTGGCCGTGG TGGCCAACGC GAAGCCACAC 
AAAGTTTACA TGGGGCCAGG GCAGCTGTAC CAGGATCTGC AGAACCTACT GCACGACCTG 
AACGTTGTTG GCCAGATCTC GCAGCTGATA GGAAACCTCC GAGGGAGCTA TCAGAACTTA 
AACCAGTCTG TAGCCCATGA TTGGACGTCG GGCTTACAGC GGCTGATACT GAGGAAAGAA 
GATGCGATCA GAGCTGCCGA CCGCTGCCGG ATCCAGTTAC AGCTCCCGGG AAAGCAGGAC 
AAGTCCGGGC GGCCCACGTT CTTCACTGCT GTGTTCAATA CGCTTACACC AGCCATCAAG 
GAGTCCTGGG TCAGCAGCCT GCAGATGGCC AAGCTGGCCC TTGAGGAGGA GAACCATATG 
GGATGGTTCT GTGTGGATGA CGATGGCAAC CTAGCCAAGA AGGAGACACA CCCTCTCCTG 
GTGGGACACA TGCCTGTGAT GGTGGCCAAG CAGCCAGAGT TCAAGATTGA ATGTGCAGCT 
TATAACCCTG AACCTTACCT GAGCAATGAA AGTCAACCAG CCTCACCCTC CACAGCCCGT 
GGCTTTCTGT GGATTGGAAG CTGTAGCAAC CAGATGGGTC AGGTCGCCAT TGTCTCCTTC 
CAAGGTTCCA ACCCCAAAGT CATCGAGTGC TTCAATGTGG AATCACGCAT CCTGTGTATG 
GTATACATTC CCGCAGAGGA GTCAAAGCCC CAAGAAACCA CCGAAACCAA GGATCCAGAA 
GCCACAGCCT CGAGAGCACC CCATGTGCCC ACCATCTGCC TGGGCACAGA AGAGGGAAGC 
ATTTCCATTT ACAAAAGCAG TCAGGGCTGC AAGAAGGTGA GGCTTCAGCA CTTCTATGCA 
CCGGACAAGT CCACGGTCAT GAGCTTGGCC TGCTCCCCAC AAGGCCTGTA TGCAGGCCTG 
GTCAATGGGT CCGTAGCCAG CTACACCAAG GCTCCAGCAC CGAGCTCTCC TGGCAGCCCG 
TGGGCAGAGT TCTGTCTCAT CTGCGTTTTC CTGCCATGCT TGCCCTCGGG AGCATATGCC 
ATCTCGGGTA CAGGCTGTGG TGCAGTGAAG GGCTATGGAT CCTGGAACTC GGAACCCCAG 
CAAGTGATCA AGTTGGGTGT CCTGCCAGTC AGAAGCCTTC TCCTGGTGGA GGGTGCACTG 
TGGGCAGCCT CGGGAGGGCA GGTGTTCATG GCCAGCGTGG AGACTCACAC TATAGAGAAT 
CAGCTGGAGG CCCACCAAGA TGAAGGGATG GTCATCTCCC ACATGGCTGT GGCTGGCGTT 
GGCATCTGGA TTGCCTTCAC CTCGGGGTCC ACCCTCCGCC TCTTCCACAC AGAGACCTTG 
AAGCACCTGC AGGATGTCAA CATTGATGCC CCTGTGCATA GCATGCTGCC AGGGCACCAG 
CGTCTATCTG TGACCAGCCT GCTCGTCTGC CACGGCTTGC TCATGGTCGG TACCAGCCTG 
GGTGTCGTGG TGGCCCTGCC AGTCCCTCGG CTTCAGGGTA TCCCCAAAGT GACAGGGCGA 
GGCATGGTCT CCTACCATGC GCATAACGGG CCCGTTAAGT TCATCGTCTC AGCCACGGCG 
TTTCAAAACA AGGACAGAGC CAGGGACAGC CCACGTTCCG GTTCTGAGCT TCAGGATGAA 
GACCCCAAGG ACTTGCTCTG CAGCGAGGAA GGTCCTTCCT GCCCGGGCCA GCCTGACACC 
TACACATCCG TTTGGTTGGG AGACTCCCTA GGATTGCCAA CTCAAAAGAA TGATCTGTCT 
TCGTCCTCGG GGTCACTGAA CCTGTCCCAT GGCTCCAGTT CCCTAGAGCA CAGATCTGTG 
GACAGCAGTC TGTGTGACCT GCTGAGGGAC CCCAGTGCCT CCCCAAGGAG CAGACCACAG 
GGCTCTAGGC GAGCAAGAGC CAGTTCAGCG CTGGTGGTAT GTGGGGGCCA GGGCCACCGG 
CGGGTGCACA GGAAGGCCAG GCAGCCTTCT CAGGAGGACC TGGTTTCTTC AGTGATGGTG 
TGGCAGATCC CCCTGCTGGG CATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_017312669.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168158.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X3, mRNA.

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CloneID	OMu82407
Clone ID Related Accession (Same CDS sequence)	XM_017312669.2 , XM_017312669.1
Accession Version	XM_017312669.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGAG TGTCTGTCTT GTGTTCCCTG CCAGCGACAG CGCAGCTCCC AGTGTCTAAC 
AGAGGGCGGA TGATCGGGAG ACACAATCTG GCCCCGCGCC ATTTCCCAGT GACCAGGAGT 
CTGAGGGGAG AAGCGTCCAC TCTGGGGCAC TACAAGCTGG TGTCCTTTCT GTGCCCCCTC 
TGTCCCCGGG GGCGCAAGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG CAGTGAGCAA GGCAGCCAAG AAGGCAGTGC CCTGGCCCGA 
TGGGCAGCCG ACCCTGCCAA CACAGCATGG ATGGAAAATC CAGAGGAAGC AATTTACGAC 
GACGTTCCGA GGGAAAACTC GGACTCTGAG CCAGATGAAA TGATTTATGA TGATGTGGAG 
AACGGCGAAG AAGGTGGAAA CAGCTCCCCA GAGTACGGGT GGAGTTCCAG CGAATTCGAA 
AGCTATGAGG AGCCAAGCGA CTCGGAGGGC AAGAACGGAA TTCCCAGGTC CTTCCTGCGC 
AGCAGTCATA AAAAGCAACT CTCTCATGAC CTAACCCGCT TTAAGGCGCA CTGTGAGGAA 
AAGATGAGAG GCTTGGTGGC CAGTACGGTG GGAGCCATGG AGATTCAGCA GGCAAAGCAG 
AGGCAGGAGC GGAAGATGCA GAAGCTTATG AAGGCAGCCA AGGAGGGCAC CAAAGATGGG 
CTGGAGAAAA CCAAAGCTGC TGTGAAGAGA GGCGGCTCCT TCATCAGGAC CAGGTCCTTG 
GTCTCTCAAG ATCACAGGTG TTATTTTGAA GAAGAGCAGA ATTTGTTCAT TGACGTGGAC 
TGCAAGCACC CGGAGGCTGT CCTCACCCCA ATGCCCGAGG GCTTGTCTCA GCAGCAGGTT 
GTGAGAAGAT ACATTCTGGG CTCCATTGTT GAGAGCGAGA AGAACTATGT AGATGCTCTC 
CGGAGGATTT TGGAGCAATA TGAGAAGCCG CTGTCTGAGA TGGAGCCCCG GCTCCTGAGT 
GACAGGAAGC TCAGGATGGT TTTCTACCGC GTCAAGGAGA TCCTGCAGTG TCACTCCATG 
TTCCAGATTG CGCTGGCCAG CCGCGTGTCC GAGTGGGATG TCGTGGAAAC AATCGGCGAC 
GTTTTTGTGG CCTCGTTCTC CAAGTCCATG GTGCTGGATG CTTACAGCGA GTACGTGAAC 
AACTTCAGCA CGGCCGTGGC TGTCCTCAAG AAGACGTGTG CCACAAAGCC TGCTTTCCTT 
GAGTTCTTAA AGCTGAGCCA GGATTCCAGT CCAGACCGAG TCACACTCCA CAGCCTTATG 
ATGCGGCCCA TCCAGAGGTT CCCACAGTTC ATCCTCCTAC TGCAGGACAT GCTGAAGAAC 
ACCGCCAAAG GACATCCTGA CAGGCTGCCC CTTCAGATGG CGCTGACGGA GCTCGAGACC 
CTGGCAGAGA AGCTAAACGA GAGAAAAAGA GATGCCGACC AGCGATGCGA AATCAAACAA 
ATAGCAAAAG CCATAAACGA GAGATACCTG AACAAGCTCC TCAGCAGTGG AAACCGGTAC 
CTCGTCCGGT CCGATGACGT CATCGAGACG GTTTACAACG ACCGGGGAGA AATCGTCAAG 
ACCAAACAAC GCCGGATCTT CATGCTGAAT GACGTGCTCA TGTGTGCCAC CGCCAGCTCA 
CGGAACTCAC ATGAAAGCCA TGCCGTGATG AGCCAGAGGT ACCTGCTCAA GTGGAGTGTC 
CCTCTAGGTC ACGTGGATGT GATTCAGTAC AATGGAGGCT CTGGCGCCGG GGAGCACTGT 
CGGCACCACG CTGCACACTC ACCTGAGAGC CTGGCCGTGG TGGCCAACGC GAAGCCACAC 
AAAGTTTACA TGGGGCCAGG GCAGCTGTAC CAGGATCTGC AGAACCTACT GCACGACCTG 
AACGTTGTTG GCCAGATCTC GCAGCTGATA GGAAACCTCC GAGGGAGCTA TCAGAACTTA 
AACCAGTCTG TAGCCCATGA TTGGACGTCG GGCTTACAGC GGCTGATACT GAGGAAAGAA 
GATGCGATCA GAGCTGCCGA CCGCTGCCGG ATCCAGTTAC AGCTCCCGGG AAAGCAGGAC 
AAGTCCGGGC GGCCCACGTT CTTCACTGCT GTGTTCAATA CGCTTACACC AGCCATCAAG 
GAGTCCTGGG TCAGCAGCCT GCAGATGGCC AAGCTGGCCC TTGAGGAGGA GAACCATATG 
GGATGGTTCT GTGTGGATGA CGATGGCAAC CTAGCCAAGA AGGAGACACA CCCTCTCCTG 
GTGGGACACA TGCCTGTGAT GGTGGCCAAG CAGCCAGAGT TCAAGATTGA ATGTGCAGCT 
TATAACCCTG AACCTTACCT GAGCAATGAA AGTCAACCAG CCTCACCCTC CACAGCCCGT 
GGCTTTCTGT GGATTGGAAG CTGTAGCAAC CAGATGGGTC AGGTCGCCAT TGTCTCCTTC 
CAAGGTTCCA ACCCCAAAGT CATCGAGTGC TTCAATGTGG AATCACGCAT CCTGTGTATG 
GTATACATTC CCGCAGAGGA GTCAAAGCCC CAAGAAACCA CCGAAACCAA GGATCCAGAA 
GCCACAGCCT CGAGAGCACC CCATGTGCCC ACCATCTGCC TGGGCACAGA AGAGGGAAGC 
ATTTCCATTT ACAAAAGCAG TCAGGGCTGC AAGAAGGTGA GGCTTCAGCA CTTCTATGCA 
CCGGACAAGT CCACGGTCAT GAGCTTGGCC TGCTCCCCAC AAGGCCTGTA TGCAGGCCTG 
GTCAATGGGT CCGTAGCCAG CTACACCAAG GCTCCAGCAC CGAGCTCTCC TGGCAGCCCG 
TGGGCAGAGT TCTGTCTCAT CTGCGTTTTC CTGCCATGCT TGCCCTCGGG AGCATATGCC 
ATCTCGGGTA CAGGCTGTGG TGCAGTGAAG GGCTATGGAT CCTGGAACTC GGAACCCCAG 
CAAGTGATCA AGTTGGGTGT CCTGCCAGTC AGAAGCCTTC TCCTGGTGGA GGGTGCACTG 
TGGGCAGCCT CGGGAGGGCA GGTGTTCATG GCCAGCGTGG AGACTCACAC TATAGAGAAT 
CAGCTGGAGG CCCACCAAGA TGAAGGGATG GTCATCTCCC ACATGGCTGT GGCTGGCGTT 
GGCATCTGGA TTGCCTTCAC CTCGGGGTCC ACCCTCCGCC TCTTCCACAC AGAGACCTTG 
AAGCACCTGC AGGATGTCAA CATTGATGCC CCTGTGCATA GCATGCTGCC AGGGCACCAG 
CGTCTATCTG TGACCAGCCT GCTCGTCTGC CACGGCTTGC TCATGGTCGG TACCAGCCTG 
GGTGTCGTGG TGGCCCTGCC AGTCCCTCGG CTTCAGGGTA TCCCCAAAGT GACAGGGCGA 
GGCATGGTCT CCTACCATGC GCATAACGGG CCCGTTAAGT TCATCGTCTC AGCCACGGCG 
TTTCAAAACA AGGACAGAGC CAGGGACAGC CCACGTTCCG GTTCTGAGCT TCAGGATGAA 
GACCCCAAGG ACTTGCTCTG CAGCGAGGAA GGTCCTTCCT GCCCGGGCCA GCCTGACACC 
TACACATCCG TTTGGTTGGG AGACTCCCTA GGATTGCCAA CTCAAAAGAA TGATCTGTCT 
TCGTCCTCGG GGTCACTGAA CCTGTCCCAT GGCTCCAGTT CCCTAGAGCA CAGATCTGTG 
GACAGCAGTC TGTGTGACCT GCTGAGGGAC CCCAGTGCCT CCCCAAGGAG CAGACCACAG 
GGCTCTAGGC GAGCAAGAGC CAGTTCAGCG CTGGTGGTAT GTGGGGGCCA GGGCCACCGG 
CGGGTGCACA GGAAGGCCAG GCAGCCTTCT CAGGAGGACC TGGTTTCTTC AGTGATGGTG 
TGGCAGATCC CCCTGCTGGG CATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168158.1
CDS	2766..7172
Translation	MGGVSVLCSLPATAQLPVSNRGRMIGRHNLAPRHFPVTRSLRGEASTLGHYKLVSFLCPLCPRGRKGPLSPDAVQAWRKEARLSMEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQLSHDLTRFKAHCEEKMRGLVASTVGAMEIQQAKQRQERKMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPAPSSPGSPWAEFCLICVFLPCLPSGAYAISGTGCGAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_017312669.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312669.1

ORF Insert Sequence:

ATGGGTGGAG TGTCTGTCTT GTGTTCCCTG CCAGCGACAG CGCAGCTCCC AGTGTCTAAC 
AGAGGGCGGA TGATCGGGAG ACACAATCTG GCCCCGCGCC ATTTCCCAGT GACCAGGAGT 
CTGAGGGGAG AAGCGTCCAC TCTGGGGCAC TACAAGCTGG TGTCCTTTCT GTGCCCCCTC 
TGTCCCCGGG GGCGCAAGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG CAGTGAGCAA GGCAGCCAAG AAGGCAGTGC CCTGGCCCGA 
TGGGCAGCCG ACCCTGCCAA CACAGCATGG ATGGAAAATC CAGAGGAAGC AATTTACGAC 
GACGTTCCGA GGGAAAACTC GGACTCTGAG CCAGATGAAA TGATTTATGA TGATGTGGAG 
AACGGCGAAG AAGGTGGAAA CAGCTCCCCA GAGTACGGGT GGAGTTCCAG CGAATTCGAA 
AGCTATGAGG AGCCAAGCGA CTCGGAGGGC AAGAACGGAA TTCCCAGGTC CTTCCTGCGC 
AGCAGTCATA AAAAGCAACT CTCTCATGAC CTAACCCGCT TTAAGGCGCA CTGTGAGGAA 
AAGATGAGAG GCTTGGTGGC CAGTACGGTG GGAGCCATGG AGATTCAGCA GGCAAAGCAG 
AGGCAGGAGC GGAAGATGCA GAAGCTTATG AAGGCAGCCA AGGAGGGCAC CAAAGATGGG 
CTGGAGAAAA CCAAAGCTGC TGTGAAGAGA GGCGGCTCCT TCATCAGGAC CAGGTCCTTG 
GTCTCTCAAG ATCACAGGTG TTATTTTGAA GAAGAGCAGA ATTTGTTCAT TGACGTGGAC 
TGCAAGCACC CGGAGGCTGT CCTCACCCCA ATGCCCGAGG GCTTGTCTCA GCAGCAGGTT 
GTGAGAAGAT ACATTCTGGG CTCCATTGTT GAGAGCGAGA AGAACTATGT AGATGCTCTC 
CGGAGGATTT TGGAGCAATA TGAGAAGCCG CTGTCTGAGA TGGAGCCCCG GCTCCTGAGT 
GACAGGAAGC TCAGGATGGT TTTCTACCGC GTCAAGGAGA TCCTGCAGTG TCACTCCATG 
TTCCAGATTG CGCTGGCCAG CCGCGTGTCC GAGTGGGATG TCGTGGAAAC AATCGGCGAC 
GTTTTTGTGG CCTCGTTCTC CAAGTCCATG GTGCTGGATG CTTACAGCGA GTACGTGAAC 
AACTTCAGCA CGGCCGTGGC TGTCCTCAAG AAGACGTGTG CCACAAAGCC TGCTTTCCTT 
GAGTTCTTAA AGCTGAGCCA GGATTCCAGT CCAGACCGAG TCACACTCCA CAGCCTTATG 
ATGCGGCCCA TCCAGAGGTT CCCACAGTTC ATCCTCCTAC TGCAGGACAT GCTGAAGAAC 
ACCGCCAAAG GACATCCTGA CAGGCTGCCC CTTCAGATGG CGCTGACGGA GCTCGAGACC 
CTGGCAGAGA AGCTAAACGA GAGAAAAAGA GATGCCGACC AGCGATGCGA AATCAAACAA 
ATAGCAAAAG CCATAAACGA GAGATACCTG AACAAGCTCC TCAGCAGTGG AAACCGGTAC 
CTCGTCCGGT CCGATGACGT CATCGAGACG GTTTACAACG ACCGGGGAGA AATCGTCAAG 
ACCAAACAAC GCCGGATCTT CATGCTGAAT GACGTGCTCA TGTGTGCCAC CGCCAGCTCA 
CGGAACTCAC ATGAAAGCCA TGCCGTGATG AGCCAGAGGT ACCTGCTCAA GTGGAGTGTC 
CCTCTAGGTC ACGTGGATGT GATTCAGTAC AATGGAGGCT CTGGCGCCGG GGAGCACTGT 
CGGCACCACG CTGCACACTC ACCTGAGAGC CTGGCCGTGG TGGCCAACGC GAAGCCACAC 
AAAGTTTACA TGGGGCCAGG GCAGCTGTAC CAGGATCTGC AGAACCTACT GCACGACCTG 
AACGTTGTTG GCCAGATCTC GCAGCTGATA GGAAACCTCC GAGGGAGCTA TCAGAACTTA 
AACCAGTCTG TAGCCCATGA TTGGACGTCG GGCTTACAGC GGCTGATACT GAGGAAAGAA 
GATGCGATCA GAGCTGCCGA CCGCTGCCGG ATCCAGTTAC AGCTCCCGGG AAAGCAGGAC 
AAGTCCGGGC GGCCCACGTT CTTCACTGCT GTGTTCAATA CGCTTACACC AGCCATCAAG 
GAGTCCTGGG TCAGCAGCCT GCAGATGGCC AAGCTGGCCC TTGAGGAGGA GAACCATATG 
GGATGGTTCT GTGTGGATGA CGATGGCAAC CTAGCCAAGA AGGAGACACA CCCTCTCCTG 
GTGGGACACA TGCCTGTGAT GGTGGCCAAG CAGCCAGAGT TCAAGATTGA ATGTGCAGCT 
TATAACCCTG AACCTTACCT GAGCAATGAA AGTCAACCAG CCTCACCCTC CACAGCCCGT 
GGCTTTCTGT GGATTGGAAG CTGTAGCAAC CAGATGGGTC AGGTCGCCAT TGTCTCCTTC 
CAAGGTTCCA ACCCCAAAGT CATCGAGTGC TTCAATGTGG AATCACGCAT CCTGTGTATG 
GTATACATTC CCGCAGAGGA GTCAAAGCCC CAAGAAACCA CCGAAACCAA GGATCCAGAA 
GCCACAGCCT CGAGAGCACC CCATGTGCCC ACCATCTGCC TGGGCACAGA AGAGGGAAGC 
ATTTCCATTT ACAAAAGCAG TCAGGGCTGC AAGAAGGTGA GGCTTCAGCA CTTCTATGCA 
CCGGACAAGT CCACGGTCAT GAGCTTGGCC TGCTCCCCAC AAGGCCTGTA TGCAGGCCTG 
GTCAATGGGT CCGTAGCCAG CTACACCAAG GCTCCAGCAC CGAGCTCTCC TGGCAGCCCG 
TGGGCAGAGT TCTGTCTCAT CTGCGTTTTC CTGCCATGCT TGCCCTCGGG AGCATATGCC 
ATCTCGGGTA CAGGCTGTGG TGCAGTGAAG GGCTATGGAT CCTGGAACTC GGAACCCCAG 
CAAGTGATCA AGTTGGGTGT CCTGCCAGTC AGAAGCCTTC TCCTGGTGGA GGGTGCACTG 
TGGGCAGCCT CGGGAGGGCA GGTGTTCATG GCCAGCGTGG AGACTCACAC TATAGAGAAT 
CAGCTGGAGG CCCACCAAGA TGAAGGGATG GTCATCTCCC ACATGGCTGT GGCTGGCGTT 
GGCATCTGGA TTGCCTTCAC CTCGGGGTCC ACCCTCCGCC TCTTCCACAC AGAGACCTTG 
AAGCACCTGC AGGATGTCAA CATTGATGCC CCTGTGCATA GCATGCTGCC AGGGCACCAG 
CGTCTATCTG TGACCAGCCT GCTCGTCTGC CACGGCTTGC TCATGGTCGG TACCAGCCTG 
GGTGTCGTGG TGGCCCTGCC AGTCCCTCGG CTTCAGGGTA TCCCCAAAGT GACAGGGCGA 
GGCATGGTCT CCTACCATGC GCATAACGGG CCCGTTAAGT TCATCGTCTC AGCCACGGCG 
TTTCAAAACA AGGACAGAGC CAGGGACAGC CCACGTTCCG GTTCTGAGCT TCAGGATGAA 
GACCCCAAGG ACTTGCTCTG CAGCGAGGAA GGTCCTTCCT GCCCGGGCCA GCCTGACACC 
TACACATCCG TTTGGTTGGG AGACTCCCTA GGATTGCCAA CTCAAAAGAA TGATCTGTCT 
TCGTCCTCGG GGTCACTGAA CCTGTCCCAT GGCTCCAGTT CCCTAGAGCA CAGATCTGTG 
GACAGCAGTC TGTGTGACCT GCTGAGGGAC CCCAGTGCCT CCCCAAGGAG CAGACCACAG 
GGCTCTAGGC GAGCAAGAGC CAGTTCAGCG CTGGTGGTAT GTGGGGGCCA GGGCCACCGG 
CGGGTGCACA GGAAGGCCAG GCAGCCTTCT CAGGAGGACC TGGTTTCTTC AGTGATGGTG 
TGGCAGATCC CCCTGCTGGG CATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_017312670.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168159.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X4, mRNA.

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CloneID	OMu82408
Clone ID Related Accession (Same CDS sequence)	XM_017312670.2 , XM_017312670.1
Accession Version	XM_017312670.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312670.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCG GTCCCTCGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG CAGTGAGCAA GGCAGCCAAG AAGGCAGTGC CCTGGCCCGA 
TGGGCAGCCG ACCCTGCCAA CACAGCATGG ATGGAAAATC CAGAGGAAGC AATTTACGAC 
GACGTTCCGA GGGAAAACTC GGACTCTGAG CCAGATGAAA TGATTTATGA TGATGTGGAG 
AACGGCGAAG AAGGTGGAAA CAGCTCCCCA GAGTACGGGT GGAGTTCCAG CGAATTCGAA 
AGCTATGAGG AGCCAAGCGA CTCGGAGGGC AAGAACGGAA TTCCCAGGTC CTTCCTGCGC 
AGCAGTCATA AAAAGCAACT CTCTCATGAC CTAACCCGCT TTAAGGCGCA CTGTGAGGAA 
AAGATGAGAG GCTTGGTGGC CAGTACGGTG GGAGCCATGG AGATTCAGCA GGCAAAGCAG 
AGGCAGGAGC GGAAGATGCA GAAGCTTATG AAGGCAGCCA AGGAGGGCAC CAAAGATGGG 
CTGGAGAAAA CCAAAGCTGC TGTGAAGAGA GGCGGCTCCT TCATCAGGAC CAGGTCCTTG 
GTCTCTCAAG ATCACAGGTG TTATTTTGAA GAAGAGCAGA ATTTGTTCAT TGACGTGGAC 
TGCAAGCACC CGGAGGCTGT CCTCACCCCA ATGCCCGAGG GCTTGTCTCA GCAGCAGGTT 
GTGAGAAGAT ACATTCTGGG CTCCATTGTT GAGAGCGAGA AGAACTATGT AGATGCTCTC 
CGGAGGATTT TGGAGCAATA TGAGAAGCCG CTGTCTGAGA TGGAGCCCCG GCTCCTGAGT 
GACAGGAAGC TCAGGATGGT TTTCTACCGC GTCAAGGAGA TCCTGCAGTG TCACTCCATG 
TTCCAGATTG CGCTGGCCAG CCGCGTGTCC GAGTGGGATG TCGTGGAAAC AATCGGCGAC 
GTTTTTGTGG CCTCGTTCTC CAAGTCCATG GTGCTGGATG CTTACAGCGA GTACGTGAAC 
AACTTCAGCA CGGCCGTGGC TGTCCTCAAG AAGACGTGTG CCACAAAGCC TGCTTTCCTT 
GAGTTCTTAA AGCTGAGCCA GGATTCCAGT CCAGACCGAG TCACACTCCA CAGCCTTATG 
ATGCGGCCCA TCCAGAGGTT CCCACAGTTC ATCCTCCTAC TGCAGGACAT GCTGAAGAAC 
ACCGCCAAAG GACATCCTGA CAGGCTGCCC CTTCAGATGG CGCTGACGGA GCTCGAGACC 
CTGGCAGAGA AGCTAAACGA GAGAAAAAGA GATGCCGACC AGCGATGCGA AATCAAACAA 
ATAGCAAAAG CCATAAACGA GAGATACCTG AACAAGCTCC TCAGCAGTGG AAACCGGTAC 
CTCGTCCGGT CCGATGACGT CATCGAGACG GTTTACAACG ACCGGGGAGA AATCGTCAAG 
ACCAAACAAC GCCGGATCTT CATGCTGAAT GACGTGCTCA TGTGTGCCAC CGCCAGCTCA 
CGGAACTCAC ATGAAAGCCA TGCCGTGATG AGCCAGAGGT ACCTGCTCAA GTGGAGTGTC 
CCTCTAGGTC ACGTGGATGT GATTCAGTAC AATGGAGGCT CTGGCGCCGG GGAGCACTGT 
CGGCACCACG CTGCACACTC ACCTGAGAGC CTGGCCGTGG TGGCCAACGC GAAGCCACAC 
AAAGTTTACA TGGGGCCAGG GCAGCTGTAC CAGGATCTGC AGAACCTACT GCACGACCTG 
AACGTTGTTG GCCAGATCTC GCAGCTGATA GGAAACCTCC GAGGGAGCTA TCAGAACTTA 
AACCAGTCTG TAGCCCATGA TTGGACGTCG GGCTTACAGC GGCTGATACT GAGGAAAGAA 
GATGCGATCA GAGCTGCCGA CCGCTGCCGG ATCCAGTTAC AGCTCCCGGG AAAGCAGGAC 
AAGTCCGGGC GGCCCACGTT CTTCACTGCT GTGTTCAATA CGCTTACACC AGCCATCAAG 
GAGTCCTGGG TCAGCAGCCT GCAGATGGCC AAGCTGGCCC TTGAGGAGGA GAACCATATG 
GGATGGTTCT GTGTGGATGA CGATGGCAAC CTAGCCAAGA AGGAGACACA CCCTCTCCTG 
GTGGGACACA TGCCTGTGAT GGTGGCCAAG CAGCCAGAGT TCAAGATTGA ATGTGCAGCT 
TATAACCCTG AACCTTACCT GAGCAATGAA AGTCAACCAG CCTCACCCTC CACAGCCCGT 
GGCTTTCTGT GGATTGGAAG CTGTAGCAAC CAGATGGGTC AGGTCGCCAT TGTCTCCTTC 
CAAGGTTCCA ACCCCAAAGT CATCGAGTGC TTCAATGTGG AATCACGCAT CCTGTGTATG 
GTATACATTC CCGCAGAGGA GTCAAAGCCC CAAGAAACCA CCGAAACCAA GGATCCAGAA 
GCCACAGCCT CGAGAGCACC CCATGTGCCC ACCATCTGCC TGGGCACAGA AGAGGGAAGC 
ATTTCCATTT ACAAAAGCAG TCAGGGCTGC AAGAAGGTGA GGCTTCAGCA CTTCTATGCA 
CCGGACAAGT CCACGGTCAT GAGCTTGGCC TGCTCCCCAC AAGGCCTGTA TGCAGGCCTG 
GTCAATGGGT CCGTAGCCAG CTACACCAAG GCTCCAGATG GATCCTGGAA CTCGGAACCC 
CAGCAAGTGA TCAAGTTGGG TGTCCTGCCA GTCAGAAGCC TTCTCCTGGT GGAGGGTGCA 
CTGTGGGCAG CCTCGGGAGG GCAGGTGTTC ATGGCCAGCG TGGAGACTCA CACTATAGAG 
AATCAGCTGG AGGCCCACCA AGATGAAGGG ATGGTCATCT CCCACATGGC TGTGGCTGGC 
GTTGGCATCT GGATTGCCTT CACCTCGGGG TCCACCCTCC GCCTCTTCCA CACAGAGACC 
TTGAAGCACC TGCAGGATGT CAACATTGAT GCCCCTGTGC ATAGCATGCT GCCAGGGCAC 
CAGCGTCTAT CTGTGACCAG CCTGCTCGTC TGCCACGGCT TGCTCATGGT CGGTACCAGC 
CTGGGTGTCG TGGTGGCCCT GCCAGTCCCT CGGCTTCAGG GTATCCCCAA AGTGACAGGG 
CGAGGCATGG TCTCCTACCA TGCGCATAAC GGGCCCGTTA AGTTCATCGT CTCAGCCACG 
GCGTTTCAAA ACAAGGACAG AGCCAGGGAC AGCCCACGTT CCGGTTCTGA GCTTCAGGAT 
GAAGACCCCA AGGACTTGCT CTGCAGCGAG GAAGGTCCTT CCTGCCCGGG CCAGCCTGAC 
ACCTACACAT CCGTTTGGTT GGGAGACTCC CTAGGATTGC CAACTCAAAA GAATGATCTG 
TCTTCGTCCT CGGGGTCACT GAACCTGTCC CATGGCTCCA GTTCCCTAGA GCACAGATCT 
GTGGACAGCA GTCTGTGTGA CCTGCTGAGG GACCCCAGTG CCTCCCCAAG GAGCAGACCA 
CAGGGCTCTA GGCGAGCAAG AGCCAGTTCA GCGCTGGTGG TATGTGGGGG CCAGGGCCAC 
CGGCGGGTGC ACAGGAAGGC CAGGCAGCCT TCTCAGGAGG ACCTGGTTTC TTCAGTGATG 
GTGTGGCAGA TCCCCCTGCT GGGCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168159.1
CDS	78..4187
Translation	MAPGPSGPLSPDAVQAWRKEARLSMEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQLSHDLTRFKAHCEEKMRGLVASTVGAMEIQQAKQRQERKMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPDGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_017312670.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312670.2

ORF Insert Sequence:

ATGGCGCCCG GTCCCTCGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG CAGTGAGCAA GGCAGCCAAG AAGGCAGTGC CCTGGCCCGA 
TGGGCAGCCG ACCCTGCCAA CACAGCATGG ATGGAAAATC CAGAGGAAGC AATTTACGAC 
GACGTTCCGA GGGAAAACTC GGACTCTGAG CCAGATGAAA TGATTTATGA TGATGTGGAG 
AACGGCGAAG AAGGTGGAAA CAGCTCCCCA GAGTACGGGT GGAGTTCCAG CGAATTCGAA 
AGCTATGAGG AGCCAAGCGA CTCGGAGGGC AAGAACGGAA TTCCCAGGTC CTTCCTGCGC 
AGCAGTCATA AAAAGCAACT CTCTCATGAC CTAACCCGCT TTAAGGCGCA CTGTGAGGAA 
AAGATGAGAG GCTTGGTGGC CAGTACGGTG GGAGCCATGG AGATTCAGCA GGCAAAGCAG 
AGGCAGGAGC GGAAGATGCA GAAGCTTATG AAGGCAGCCA AGGAGGGCAC CAAAGATGGG 
CTGGAGAAAA CCAAAGCTGC TGTGAAGAGA GGCGGCTCCT TCATCAGGAC CAGGTCCTTG 
GTCTCTCAAG ATCACAGGTG TTATTTTGAA GAAGAGCAGA ATTTGTTCAT TGACGTGGAC 
TGCAAGCACC CGGAGGCTGT CCTCACCCCA ATGCCCGAGG GCTTGTCTCA GCAGCAGGTT 
GTGAGAAGAT ACATTCTGGG CTCCATTGTT GAGAGCGAGA AGAACTATGT AGATGCTCTC 
CGGAGGATTT TGGAGCAATA TGAGAAGCCG CTGTCTGAGA TGGAGCCCCG GCTCCTGAGT 
GACAGGAAGC TCAGGATGGT TTTCTACCGC GTCAAGGAGA TCCTGCAGTG TCACTCCATG 
TTCCAGATTG CGCTGGCCAG CCGCGTGTCC GAGTGGGATG TCGTGGAAAC AATCGGCGAC 
GTTTTTGTGG CCTCGTTCTC CAAGTCCATG GTGCTGGATG CTTACAGCGA GTACGTGAAC 
AACTTCAGCA CGGCCGTGGC TGTCCTCAAG AAGACGTGTG CCACAAAGCC TGCTTTCCTT 
GAGTTCTTAA AGCTGAGCCA GGATTCCAGT CCAGACCGAG TCACACTCCA CAGCCTTATG 
ATGCGGCCCA TCCAGAGGTT CCCACAGTTC ATCCTCCTAC TGCAGGACAT GCTGAAGAAC 
ACCGCCAAAG GACATCCTGA CAGGCTGCCC CTTCAGATGG CGCTGACGGA GCTCGAGACC 
CTGGCAGAGA AGCTAAACGA GAGAAAAAGA GATGCCGACC AGCGATGCGA AATCAAACAA 
ATAGCAAAAG CCATAAACGA GAGATACCTG AACAAGCTCC TCAGCAGTGG AAACCGGTAC 
CTCGTCCGGT CCGATGACGT CATCGAGACG GTTTACAACG ACCGGGGAGA AATCGTCAAG 
ACCAAACAAC GCCGGATCTT CATGCTGAAT GACGTGCTCA TGTGTGCCAC CGCCAGCTCA 
CGGAACTCAC ATGAAAGCCA TGCCGTGATG AGCCAGAGGT ACCTGCTCAA GTGGAGTGTC 
CCTCTAGGTC ACGTGGATGT GATTCAGTAC AATGGAGGCT CTGGCGCCGG GGAGCACTGT 
CGGCACCACG CTGCACACTC ACCTGAGAGC CTGGCCGTGG TGGCCAACGC GAAGCCACAC 
AAAGTTTACA TGGGGCCAGG GCAGCTGTAC CAGGATCTGC AGAACCTACT GCACGACCTG 
AACGTTGTTG GCCAGATCTC GCAGCTGATA GGAAACCTCC GAGGGAGCTA TCAGAACTTA 
AACCAGTCTG TAGCCCATGA TTGGACGTCG GGCTTACAGC GGCTGATACT GAGGAAAGAA 
GATGCGATCA GAGCTGCCGA CCGCTGCCGG ATCCAGTTAC AGCTCCCGGG AAAGCAGGAC 
AAGTCCGGGC GGCCCACGTT CTTCACTGCT GTGTTCAATA CGCTTACACC AGCCATCAAG 
GAGTCCTGGG TCAGCAGCCT GCAGATGGCC AAGCTGGCCC TTGAGGAGGA GAACCATATG 
GGATGGTTCT GTGTGGATGA CGATGGCAAC CTAGCCAAGA AGGAGACACA CCCTCTCCTG 
GTGGGACACA TGCCTGTGAT GGTGGCCAAG CAGCCAGAGT TCAAGATTGA ATGTGCAGCT 
TATAACCCTG AACCTTACCT GAGCAATGAA AGTCAACCAG CCTCACCCTC CACAGCCCGT 
GGCTTTCTGT GGATTGGAAG CTGTAGCAAC CAGATGGGTC AGGTCGCCAT TGTCTCCTTC 
CAAGGTTCCA ACCCCAAAGT CATCGAGTGC TTCAATGTGG AATCACGCAT CCTGTGTATG 
GTATACATTC CCGCAGAGGA GTCAAAGCCC CAAGAAACCA CCGAAACCAA GGATCCAGAA 
GCCACAGCCT CGAGAGCACC CCATGTGCCC ACCATCTGCC TGGGCACAGA AGAGGGAAGC 
ATTTCCATTT ACAAAAGCAG TCAGGGCTGC AAGAAGGTGA GGCTTCAGCA CTTCTATGCA 
CCGGACAAGT CCACGGTCAT GAGCTTGGCC TGCTCCCCAC AAGGCCTGTA TGCAGGCCTG 
GTCAATGGGT CCGTAGCCAG CTACACCAAG GCTCCAGATG GATCCTGGAA CTCGGAACCC 
CAGCAAGTGA TCAAGTTGGG TGTCCTGCCA GTCAGAAGCC TTCTCCTGGT GGAGGGTGCA 
CTGTGGGCAG CCTCGGGAGG GCAGGTGTTC ATGGCCAGCG TGGAGACTCA CACTATAGAG 
AATCAGCTGG AGGCCCACCA AGATGAAGGG ATGGTCATCT CCCACATGGC TGTGGCTGGC 
GTTGGCATCT GGATTGCCTT CACCTCGGGG TCCACCCTCC GCCTCTTCCA CACAGAGACC 
TTGAAGCACC TGCAGGATGT CAACATTGAT GCCCCTGTGC ATAGCATGCT GCCAGGGCAC 
CAGCGTCTAT CTGTGACCAG CCTGCTCGTC TGCCACGGCT TGCTCATGGT CGGTACCAGC 
CTGGGTGTCG TGGTGGCCCT GCCAGTCCCT CGGCTTCAGG GTATCCCCAA AGTGACAGGG 
CGAGGCATGG TCTCCTACCA TGCGCATAAC GGGCCCGTTA AGTTCATCGT CTCAGCCACG 
GCGTTTCAAA ACAAGGACAG AGCCAGGGAC AGCCCACGTT CCGGTTCTGA GCTTCAGGAT 
GAAGACCCCA AGGACTTGCT CTGCAGCGAG GAAGGTCCTT CCTGCCCGGG CCAGCCTGAC 
ACCTACACAT CCGTTTGGTT GGGAGACTCC CTAGGATTGC CAACTCAAAA GAATGATCTG 
TCTTCGTCCT CGGGGTCACT GAACCTGTCC CATGGCTCCA GTTCCCTAGA GCACAGATCT 
GTGGACAGCA GTCTGTGTGA CCTGCTGAGG GACCCCAGTG CCTCCCCAAG GAGCAGACCA 
CAGGGCTCTA GGCGAGCAAG AGCCAGTTCA GCGCTGGTGG TATGTGGGGG CCAGGGCCAC 
CGGCGGGTGC ACAGGAAGGC CAGGCAGCCT TCTCAGGAGG ACCTGGTTTC TTCAGTGATG 
GTGTGGCAGA TCCCCCTGCT GGGCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_017312670.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168159.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X4, mRNA.

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CloneID	OMu82408
Clone ID Related Accession (Same CDS sequence)	XM_017312670.2 , XM_017312670.1
Accession Version	XM_017312670.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCG GTCCCTCGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG CAGTGAGCAA GGCAGCCAAG AAGGCAGTGC CCTGGCCCGA 
TGGGCAGCCG ACCCTGCCAA CACAGCATGG ATGGAAAATC CAGAGGAAGC AATTTACGAC 
GACGTTCCGA GGGAAAACTC GGACTCTGAG CCAGATGAAA TGATTTATGA TGATGTGGAG 
AACGGCGAAG AAGGTGGAAA CAGCTCCCCA GAGTACGGGT GGAGTTCCAG CGAATTCGAA 
AGCTATGAGG AGCCAAGCGA CTCGGAGGGC AAGAACGGAA TTCCCAGGTC CTTCCTGCGC 
AGCAGTCATA AAAAGCAACT CTCTCATGAC CTAACCCGCT TTAAGGCGCA CTGTGAGGAA 
AAGATGAGAG GCTTGGTGGC CAGTACGGTG GGAGCCATGG AGATTCAGCA GGCAAAGCAG 
AGGCAGGAGC GGAAGATGCA GAAGCTTATG AAGGCAGCCA AGGAGGGCAC CAAAGATGGG 
CTGGAGAAAA CCAAAGCTGC TGTGAAGAGA GGCGGCTCCT TCATCAGGAC CAGGTCCTTG 
GTCTCTCAAG ATCACAGGTG TTATTTTGAA GAAGAGCAGA ATTTGTTCAT TGACGTGGAC 
TGCAAGCACC CGGAGGCTGT CCTCACCCCA ATGCCCGAGG GCTTGTCTCA GCAGCAGGTT 
GTGAGAAGAT ACATTCTGGG CTCCATTGTT GAGAGCGAGA AGAACTATGT AGATGCTCTC 
CGGAGGATTT TGGAGCAATA TGAGAAGCCG CTGTCTGAGA TGGAGCCCCG GCTCCTGAGT 
GACAGGAAGC TCAGGATGGT TTTCTACCGC GTCAAGGAGA TCCTGCAGTG TCACTCCATG 
TTCCAGATTG CGCTGGCCAG CCGCGTGTCC GAGTGGGATG TCGTGGAAAC AATCGGCGAC 
GTTTTTGTGG CCTCGTTCTC CAAGTCCATG GTGCTGGATG CTTACAGCGA GTACGTGAAC 
AACTTCAGCA CGGCCGTGGC TGTCCTCAAG AAGACGTGTG CCACAAAGCC TGCTTTCCTT 
GAGTTCTTAA AGCTGAGCCA GGATTCCAGT CCAGACCGAG TCACACTCCA CAGCCTTATG 
ATGCGGCCCA TCCAGAGGTT CCCACAGTTC ATCCTCCTAC TGCAGGACAT GCTGAAGAAC 
ACCGCCAAAG GACATCCTGA CAGGCTGCCC CTTCAGATGG CGCTGACGGA GCTCGAGACC 
CTGGCAGAGA AGCTAAACGA GAGAAAAAGA GATGCCGACC AGCGATGCGA AATCAAACAA 
ATAGCAAAAG CCATAAACGA GAGATACCTG AACAAGCTCC TCAGCAGTGG AAACCGGTAC 
CTCGTCCGGT CCGATGACGT CATCGAGACG GTTTACAACG ACCGGGGAGA AATCGTCAAG 
ACCAAACAAC GCCGGATCTT CATGCTGAAT GACGTGCTCA TGTGTGCCAC CGCCAGCTCA 
CGGAACTCAC ATGAAAGCCA TGCCGTGATG AGCCAGAGGT ACCTGCTCAA GTGGAGTGTC 
CCTCTAGGTC ACGTGGATGT GATTCAGTAC AATGGAGGCT CTGGCGCCGG GGAGCACTGT 
CGGCACCACG CTGCACACTC ACCTGAGAGC CTGGCCGTGG TGGCCAACGC GAAGCCACAC 
AAAGTTTACA TGGGGCCAGG GCAGCTGTAC CAGGATCTGC AGAACCTACT GCACGACCTG 
AACGTTGTTG GCCAGATCTC GCAGCTGATA GGAAACCTCC GAGGGAGCTA TCAGAACTTA 
AACCAGTCTG TAGCCCATGA TTGGACGTCG GGCTTACAGC GGCTGATACT GAGGAAAGAA 
GATGCGATCA GAGCTGCCGA CCGCTGCCGG ATCCAGTTAC AGCTCCCGGG AAAGCAGGAC 
AAGTCCGGGC GGCCCACGTT CTTCACTGCT GTGTTCAATA CGCTTACACC AGCCATCAAG 
GAGTCCTGGG TCAGCAGCCT GCAGATGGCC AAGCTGGCCC TTGAGGAGGA GAACCATATG 
GGATGGTTCT GTGTGGATGA CGATGGCAAC CTAGCCAAGA AGGAGACACA CCCTCTCCTG 
GTGGGACACA TGCCTGTGAT GGTGGCCAAG CAGCCAGAGT TCAAGATTGA ATGTGCAGCT 
TATAACCCTG AACCTTACCT GAGCAATGAA AGTCAACCAG CCTCACCCTC CACAGCCCGT 
GGCTTTCTGT GGATTGGAAG CTGTAGCAAC CAGATGGGTC AGGTCGCCAT TGTCTCCTTC 
CAAGGTTCCA ACCCCAAAGT CATCGAGTGC TTCAATGTGG AATCACGCAT CCTGTGTATG 
GTATACATTC CCGCAGAGGA GTCAAAGCCC CAAGAAACCA CCGAAACCAA GGATCCAGAA 
GCCACAGCCT CGAGAGCACC CCATGTGCCC ACCATCTGCC TGGGCACAGA AGAGGGAAGC 
ATTTCCATTT ACAAAAGCAG TCAGGGCTGC AAGAAGGTGA GGCTTCAGCA CTTCTATGCA 
CCGGACAAGT CCACGGTCAT GAGCTTGGCC TGCTCCCCAC AAGGCCTGTA TGCAGGCCTG 
GTCAATGGGT CCGTAGCCAG CTACACCAAG GCTCCAGATG GATCCTGGAA CTCGGAACCC 
CAGCAAGTGA TCAAGTTGGG TGTCCTGCCA GTCAGAAGCC TTCTCCTGGT GGAGGGTGCA 
CTGTGGGCAG CCTCGGGAGG GCAGGTGTTC ATGGCCAGCG TGGAGACTCA CACTATAGAG 
AATCAGCTGG AGGCCCACCA AGATGAAGGG ATGGTCATCT CCCACATGGC TGTGGCTGGC 
GTTGGCATCT GGATTGCCTT CACCTCGGGG TCCACCCTCC GCCTCTTCCA CACAGAGACC 
TTGAAGCACC TGCAGGATGT CAACATTGAT GCCCCTGTGC ATAGCATGCT GCCAGGGCAC 
CAGCGTCTAT CTGTGACCAG CCTGCTCGTC TGCCACGGCT TGCTCATGGT CGGTACCAGC 
CTGGGTGTCG TGGTGGCCCT GCCAGTCCCT CGGCTTCAGG GTATCCCCAA AGTGACAGGG 
CGAGGCATGG TCTCCTACCA TGCGCATAAC GGGCCCGTTA AGTTCATCGT CTCAGCCACG 
GCGTTTCAAA ACAAGGACAG AGCCAGGGAC AGCCCACGTT CCGGTTCTGA GCTTCAGGAT 
GAAGACCCCA AGGACTTGCT CTGCAGCGAG GAAGGTCCTT CCTGCCCGGG CCAGCCTGAC 
ACCTACACAT CCGTTTGGTT GGGAGACTCC CTAGGATTGC CAACTCAAAA GAATGATCTG 
TCTTCGTCCT CGGGGTCACT GAACCTGTCC CATGGCTCCA GTTCCCTAGA GCACAGATCT 
GTGGACAGCA GTCTGTGTGA CCTGCTGAGG GACCCCAGTG CCTCCCCAAG GAGCAGACCA 
CAGGGCTCTA GGCGAGCAAG AGCCAGTTCA GCGCTGGTGG TATGTGGGGG CCAGGGCCAC 
CGGCGGGTGC ACAGGAAGGC CAGGCAGCCT TCTCAGGAGG ACCTGGTTTC TTCAGTGATG 
GTGTGGCAGA TCCCCCTGCT GGGCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168159.1
CDS	77..4186
Translation	MAPGPSGPLSPDAVQAWRKEARLSMEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQLSHDLTRFKAHCEEKMRGLVASTVGAMEIQQAKQRQERKMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPDGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_017312670.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312670.1

ORF Insert Sequence:

ATGGCGCCCG GTCCCTCGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG CAGTGAGCAA GGCAGCCAAG AAGGCAGTGC CCTGGCCCGA 
TGGGCAGCCG ACCCTGCCAA CACAGCATGG ATGGAAAATC CAGAGGAAGC AATTTACGAC 
GACGTTCCGA GGGAAAACTC GGACTCTGAG CCAGATGAAA TGATTTATGA TGATGTGGAG 
AACGGCGAAG AAGGTGGAAA CAGCTCCCCA GAGTACGGGT GGAGTTCCAG CGAATTCGAA 
AGCTATGAGG AGCCAAGCGA CTCGGAGGGC AAGAACGGAA TTCCCAGGTC CTTCCTGCGC 
AGCAGTCATA AAAAGCAACT CTCTCATGAC CTAACCCGCT TTAAGGCGCA CTGTGAGGAA 
AAGATGAGAG GCTTGGTGGC CAGTACGGTG GGAGCCATGG AGATTCAGCA GGCAAAGCAG 
AGGCAGGAGC GGAAGATGCA GAAGCTTATG AAGGCAGCCA AGGAGGGCAC CAAAGATGGG 
CTGGAGAAAA CCAAAGCTGC TGTGAAGAGA GGCGGCTCCT TCATCAGGAC CAGGTCCTTG 
GTCTCTCAAG ATCACAGGTG TTATTTTGAA GAAGAGCAGA ATTTGTTCAT TGACGTGGAC 
TGCAAGCACC CGGAGGCTGT CCTCACCCCA ATGCCCGAGG GCTTGTCTCA GCAGCAGGTT 
GTGAGAAGAT ACATTCTGGG CTCCATTGTT GAGAGCGAGA AGAACTATGT AGATGCTCTC 
CGGAGGATTT TGGAGCAATA TGAGAAGCCG CTGTCTGAGA TGGAGCCCCG GCTCCTGAGT 
GACAGGAAGC TCAGGATGGT TTTCTACCGC GTCAAGGAGA TCCTGCAGTG TCACTCCATG 
TTCCAGATTG CGCTGGCCAG CCGCGTGTCC GAGTGGGATG TCGTGGAAAC AATCGGCGAC 
GTTTTTGTGG CCTCGTTCTC CAAGTCCATG GTGCTGGATG CTTACAGCGA GTACGTGAAC 
AACTTCAGCA CGGCCGTGGC TGTCCTCAAG AAGACGTGTG CCACAAAGCC TGCTTTCCTT 
GAGTTCTTAA AGCTGAGCCA GGATTCCAGT CCAGACCGAG TCACACTCCA CAGCCTTATG 
ATGCGGCCCA TCCAGAGGTT CCCACAGTTC ATCCTCCTAC TGCAGGACAT GCTGAAGAAC 
ACCGCCAAAG GACATCCTGA CAGGCTGCCC CTTCAGATGG CGCTGACGGA GCTCGAGACC 
CTGGCAGAGA AGCTAAACGA GAGAAAAAGA GATGCCGACC AGCGATGCGA AATCAAACAA 
ATAGCAAAAG CCATAAACGA GAGATACCTG AACAAGCTCC TCAGCAGTGG AAACCGGTAC 
CTCGTCCGGT CCGATGACGT CATCGAGACG GTTTACAACG ACCGGGGAGA AATCGTCAAG 
ACCAAACAAC GCCGGATCTT CATGCTGAAT GACGTGCTCA TGTGTGCCAC CGCCAGCTCA 
CGGAACTCAC ATGAAAGCCA TGCCGTGATG AGCCAGAGGT ACCTGCTCAA GTGGAGTGTC 
CCTCTAGGTC ACGTGGATGT GATTCAGTAC AATGGAGGCT CTGGCGCCGG GGAGCACTGT 
CGGCACCACG CTGCACACTC ACCTGAGAGC CTGGCCGTGG TGGCCAACGC GAAGCCACAC 
AAAGTTTACA TGGGGCCAGG GCAGCTGTAC CAGGATCTGC AGAACCTACT GCACGACCTG 
AACGTTGTTG GCCAGATCTC GCAGCTGATA GGAAACCTCC GAGGGAGCTA TCAGAACTTA 
AACCAGTCTG TAGCCCATGA TTGGACGTCG GGCTTACAGC GGCTGATACT GAGGAAAGAA 
GATGCGATCA GAGCTGCCGA CCGCTGCCGG ATCCAGTTAC AGCTCCCGGG AAAGCAGGAC 
AAGTCCGGGC GGCCCACGTT CTTCACTGCT GTGTTCAATA CGCTTACACC AGCCATCAAG 
GAGTCCTGGG TCAGCAGCCT GCAGATGGCC AAGCTGGCCC TTGAGGAGGA GAACCATATG 
GGATGGTTCT GTGTGGATGA CGATGGCAAC CTAGCCAAGA AGGAGACACA CCCTCTCCTG 
GTGGGACACA TGCCTGTGAT GGTGGCCAAG CAGCCAGAGT TCAAGATTGA ATGTGCAGCT 
TATAACCCTG AACCTTACCT GAGCAATGAA AGTCAACCAG CCTCACCCTC CACAGCCCGT 
GGCTTTCTGT GGATTGGAAG CTGTAGCAAC CAGATGGGTC AGGTCGCCAT TGTCTCCTTC 
CAAGGTTCCA ACCCCAAAGT CATCGAGTGC TTCAATGTGG AATCACGCAT CCTGTGTATG 
GTATACATTC CCGCAGAGGA GTCAAAGCCC CAAGAAACCA CCGAAACCAA GGATCCAGAA 
GCCACAGCCT CGAGAGCACC CCATGTGCCC ACCATCTGCC TGGGCACAGA AGAGGGAAGC 
ATTTCCATTT ACAAAAGCAG TCAGGGCTGC AAGAAGGTGA GGCTTCAGCA CTTCTATGCA 
CCGGACAAGT CCACGGTCAT GAGCTTGGCC TGCTCCCCAC AAGGCCTGTA TGCAGGCCTG 
GTCAATGGGT CCGTAGCCAG CTACACCAAG GCTCCAGATG GATCCTGGAA CTCGGAACCC 
CAGCAAGTGA TCAAGTTGGG TGTCCTGCCA GTCAGAAGCC TTCTCCTGGT GGAGGGTGCA 
CTGTGGGCAG CCTCGGGAGG GCAGGTGTTC ATGGCCAGCG TGGAGACTCA CACTATAGAG 
AATCAGCTGG AGGCCCACCA AGATGAAGGG ATGGTCATCT CCCACATGGC TGTGGCTGGC 
GTTGGCATCT GGATTGCCTT CACCTCGGGG TCCACCCTCC GCCTCTTCCA CACAGAGACC 
TTGAAGCACC TGCAGGATGT CAACATTGAT GCCCCTGTGC ATAGCATGCT GCCAGGGCAC 
CAGCGTCTAT CTGTGACCAG CCTGCTCGTC TGCCACGGCT TGCTCATGGT CGGTACCAGC 
CTGGGTGTCG TGGTGGCCCT GCCAGTCCCT CGGCTTCAGG GTATCCCCAA AGTGACAGGG 
CGAGGCATGG TCTCCTACCA TGCGCATAAC GGGCCCGTTA AGTTCATCGT CTCAGCCACG 
GCGTTTCAAA ACAAGGACAG AGCCAGGGAC AGCCCACGTT CCGGTTCTGA GCTTCAGGAT 
GAAGACCCCA AGGACTTGCT CTGCAGCGAG GAAGGTCCTT CCTGCCCGGG CCAGCCTGAC 
ACCTACACAT CCGTTTGGTT GGGAGACTCC CTAGGATTGC CAACTCAAAA GAATGATCTG 
TCTTCGTCCT CGGGGTCACT GAACCTGTCC CATGGCTCCA GTTCCCTAGA GCACAGATCT 
GTGGACAGCA GTCTGTGTGA CCTGCTGAGG GACCCCAGTG CCTCCCCAAG GAGCAGACCA 
CAGGGCTCTA GGCGAGCAAG AGCCAGTTCA GCGCTGGTGG TATGTGGGGG CCAGGGCCAC 
CGGCGGGTGC ACAGGAAGGC CAGGCAGCCT TCTCAGGAGG ACCTGGTTTC TTCAGTGATG 
GTGTGGCAGA TCCCCCTGCT GGGCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_017312671.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168160.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu82409
Clone ID Related Accession (Same CDS sequence)	XM_017312671.1
Accession Version	XM_017312671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCG GTCCCTCGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG CAGTGAGCAA GGCAGCCAAG AAGGCAGTGC CCTGGCCCGA 
TGGGCAGCCG ACCCTGCCAA CACAGCATGG ATGGAAAATC CAGAGGAAGC AATTTACGAC 
GACGTTCCGA GGGAAAACTC GGACTCTGAG CCAGATGAAA TGATTTATGA TGATGTGGAG 
AACGGCGAAG AAGGTGGAAA CAGCTCCCCA GAGTACGGGT GGAGTTCCAG CGAATTCGAA 
AGCTATGAGG AGCCAAGCGA CTCGGAGGGC AAGAACGGAA TTCCCAGGTC CTTCCTGCGC 
AGCAGTCATA AAAAGCAAAT GCAGAAGCTT ATGAAGGCAG CCAAGGAGGG CACCAAAGAT 
GGGCTGGAGA AAACCAAAGC TGCTGTGAAG AGAGGCGGCT CCTTCATCAG GACCAGGTCC 
TTGGTCTCTC AAGATCACAG GTGTTATTTT GAAGAAGAGC AGAATTTGTT CATTGACGTG 
GACTGCAAGC ACCCGGAGGC TGTCCTCACC CCAATGCCCG AGGGCTTGTC TCAGCAGCAG 
GTTGTGAGAA GATACATTCT GGGCTCCATT GTTGAGAGCG AGAAGAACTA TGTAGATGCT 
CTCCGGAGGA TTTTGGAGCA ATATGAGAAG CCGCTGTCTG AGATGGAGCC CCGGCTCCTG 
AGTGACAGGA AGCTCAGGAT GGTTTTCTAC CGCGTCAAGG AGATCCTGCA GTGTCACTCC 
ATGTTCCAGA TTGCGCTGGC CAGCCGCGTG TCCGAGTGGG ATGTCGTGGA AACAATCGGC 
GACGTTTTTG TGGCCTCGTT CTCCAAGTCC ATGGTGCTGG ATGCTTACAG CGAGTACGTG 
AACAACTTCA GCACGGCCGT GGCTGTCCTC AAGAAGACGT GTGCCACAAA GCCTGCTTTC 
CTTGAGTTCT TAAAGCTGAG CCAGGATTCC AGTCCAGACC GAGTCACACT CCACAGCCTT 
ATGATGCGGC CCATCCAGAG GTTCCCACAG TTCATCCTCC TACTGCAGGA CATGCTGAAG 
AACACCGCCA AAGGACATCC TGACAGGCTG CCCCTTCAGA TGGCGCTGAC GGAGCTCGAG 
ACCCTGGCAG AGAAGCTAAA CGAGAGAAAA AGAGATGCCG ACCAGCGATG CGAAATCAAA 
CAAATAGCAA AAGCCATAAA CGAGAGATAC CTGAACAAGC TCCTCAGCAG TGGAAACCGG 
TACCTCGTCC GGTCCGATGA CGTCATCGAG ACGGTTTACA ACGACCGGGG AGAAATCGTC 
AAGACCAAAC AACGCCGGAT CTTCATGCTG AATGACGTGC TCATGTGTGC CACCGCCAGC 
TCACGGAACT CACATGAAAG CCATGCCGTG ATGAGCCAGA GGTACCTGCT CAAGTGGAGT 
GTCCCTCTAG GTCACGTGGA TGTGATTCAG TACAATGGAG GCTCTGGCGC CGGGGAGCAC 
TGTCGGCACC ACGCTGCACA CTCACCTGAG AGCCTGGCCG TGGTGGCCAA CGCGAAGCCA 
CACAAAGTTT ACATGGGGCC AGGGCAGCTG TACCAGGATC TGCAGAACCT ACTGCACGAC 
CTGAACGTTG TTGGCCAGAT CTCGCAGCTG ATAGGAAACC TCCGAGGGAG CTATCAGAAC 
TTAAACCAGT CTGTAGCCCA TGATTGGACG TCGGGCTTAC AGCGGCTGAT ACTGAGGAAA 
GAAGATGCGA TCAGAGCTGC CGACCGCTGC CGGATCCAGT TACAGCTCCC GGGAAAGCAG 
GACAAGTCCG GGCGGCCCAC GTTCTTCACT GCTGTGTTCA ATACGCTTAC ACCAGCCATC 
AAGGAGTCCT GGGTCAGCAG CCTGCAGATG GCCAAGCTGG CCCTTGAGGA GGAGAACCAT 
ATGGGATGGT TCTGTGTGGA TGACGATGGC AACCTAGCCA AGAAGGAGAC ACACCCTCTC 
CTGGTGGGAC ACATGCCTGT GATGGTGGCC AAGCAGCCAG AGTTCAAGAT TGAATGTGCA 
GCTTATAACC CTGAACCTTA CCTGAGCAAT GAAAGTCAAC CAGCCTCACC CTCCACAGCC 
CGTGGCTTTC TGTGGATTGG AAGCTGTAGC AACCAGATGG GTCAGGTCGC CATTGTCTCC 
TTCCAAGGTT CCAACCCCAA AGTCATCGAG TGCTTCAATG TGGAATCACG CATCCTGTGT 
ATGGTATACA TTCCCGCAGA GGAGTCAAAG CCCCAAGAAA CCACCGAAAC CAAGGATCCA 
GAAGCCACAG CCTCGAGAGC ACCCCATGTG CCCACCATCT GCCTGGGCAC AGAAGAGGGA 
AGCATTTCCA TTTACAAAAG CAGTCAGGGC TGCAAGAAGG TGAGGCTTCA GCACTTCTAT 
GCACCGGACA AGTCCACGGT CATGAGCTTG GCCTGCTCCC CACAAGGCCT GTATGCAGGC 
CTGGTCAATG GGTCCGTAGC CAGCTACACC AAGGCTCCAG CACCGAGCTC TCCTGGCAGC 
CCGTGGGCAG AGTTCTGTCT CATCTGCGTT TTCCTGCCAT GCTTGCCCTC GGGAGCATAT 
GCCATCTCGG GTACAGGCTG TGGTGCAGTG AAGGGCTATG GATCCTGGAA CTCGGAACCC 
CAGCAAGTGA TCAAGTTGGG TGTCCTGCCA GTCAGAAGCC TTCTCCTGGT GGAGGGTGCA 
CTGTGGGCAG CCTCGGGAGG GCAGGTGTTC ATGGCCAGCG TGGAGACTCA CACTATAGAG 
AATCAGCTGG AGGCCCACCA AGATGAAGGG ATGGTCATCT CCCACATGGC TGTGGCTGGC 
GTTGGCATCT GGATTGCCTT CACCTCGGGG TCCACCCTCC GCCTCTTCCA CACAGAGACC 
TTGAAGCACC TGCAGGATGT CAACATTGAT GCCCCTGTGC ATAGCATGCT GCCAGGGCAC 
CAGCGTCTAT CTGTGACCAG CCTGCTCGTC TGCCACGGCT TGCTCATGGT CGGTACCAGC 
CTGGGTGTCG TGGTGGCCCT GCCAGTCCCT CGGCTTCAGG GTATCCCCAA AGTGACAGGG 
CGAGGCATGG TCTCCTACCA TGCGCATAAC GGGCCCGTTA AGTTCATCGT CTCAGCCACG 
GCGTTTCAAA ACAAGGACAG AGCCAGGGAC AGCCCACGTT CCGGTTCTGA GCTTCAGGAT 
GAAGACCCCA AGGACTTGCT CTGCAGCGAG GAAGGTCCTT CCTGCCCGGG CCAGCCTGAC 
ACCTACACAT CCGTTTGGTT GGGAGACTCC CTAGGATTGC CAACTCAAAA GAATGATCTG 
TCTTCGTCCT CGGGGTCACT GAACCTGTCC CATGGCTCCA GTTCCCTAGA GCACAGATCT 
GTGGACAGCA GTCTGTGTGA CCTGCTGAGG GACCCCAGTG CCTCCCCAAG GAGCAGACCA 
CAGGGCTCTA GGCGAGCAAG AGCCAGTTCA GCGCTGGTGG TATGTGGGGG CCAGGGCCAC 
CGGCGGGTGC ACAGGAAGGC CAGGCAGCCT TCTCAGGAGG ACCTGGTTTC TTCAGTGATG 
GTGTGGCAGA TCCCCCTGCT GGGCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168160.1
CDS	78..4187
Translation	MAPGPSGPLSPDAVQAWRKEARLSMEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPAPSSPGSPWAEFCLICVFLPCLPSGAYAISGTGCGAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_017312671.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312671.1

ORF Insert Sequence:

ATGGCGCCCG GTCCCTCGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG CAGTGAGCAA GGCAGCCAAG AAGGCAGTGC CCTGGCCCGA 
TGGGCAGCCG ACCCTGCCAA CACAGCATGG ATGGAAAATC CAGAGGAAGC AATTTACGAC 
GACGTTCCGA GGGAAAACTC GGACTCTGAG CCAGATGAAA TGATTTATGA TGATGTGGAG 
AACGGCGAAG AAGGTGGAAA CAGCTCCCCA GAGTACGGGT GGAGTTCCAG CGAATTCGAA 
AGCTATGAGG AGCCAAGCGA CTCGGAGGGC AAGAACGGAA TTCCCAGGTC CTTCCTGCGC 
AGCAGTCATA AAAAGCAAAT GCAGAAGCTT ATGAAGGCAG CCAAGGAGGG CACCAAAGAT 
GGGCTGGAGA AAACCAAAGC TGCTGTGAAG AGAGGCGGCT CCTTCATCAG GACCAGGTCC 
TTGGTCTCTC AAGATCACAG GTGTTATTTT GAAGAAGAGC AGAATTTGTT CATTGACGTG 
GACTGCAAGC ACCCGGAGGC TGTCCTCACC CCAATGCCCG AGGGCTTGTC TCAGCAGCAG 
GTTGTGAGAA GATACATTCT GGGCTCCATT GTTGAGAGCG AGAAGAACTA TGTAGATGCT 
CTCCGGAGGA TTTTGGAGCA ATATGAGAAG CCGCTGTCTG AGATGGAGCC CCGGCTCCTG 
AGTGACAGGA AGCTCAGGAT GGTTTTCTAC CGCGTCAAGG AGATCCTGCA GTGTCACTCC 
ATGTTCCAGA TTGCGCTGGC CAGCCGCGTG TCCGAGTGGG ATGTCGTGGA AACAATCGGC 
GACGTTTTTG TGGCCTCGTT CTCCAAGTCC ATGGTGCTGG ATGCTTACAG CGAGTACGTG 
AACAACTTCA GCACGGCCGT GGCTGTCCTC AAGAAGACGT GTGCCACAAA GCCTGCTTTC 
CTTGAGTTCT TAAAGCTGAG CCAGGATTCC AGTCCAGACC GAGTCACACT CCACAGCCTT 
ATGATGCGGC CCATCCAGAG GTTCCCACAG TTCATCCTCC TACTGCAGGA CATGCTGAAG 
AACACCGCCA AAGGACATCC TGACAGGCTG CCCCTTCAGA TGGCGCTGAC GGAGCTCGAG 
ACCCTGGCAG AGAAGCTAAA CGAGAGAAAA AGAGATGCCG ACCAGCGATG CGAAATCAAA 
CAAATAGCAA AAGCCATAAA CGAGAGATAC CTGAACAAGC TCCTCAGCAG TGGAAACCGG 
TACCTCGTCC GGTCCGATGA CGTCATCGAG ACGGTTTACA ACGACCGGGG AGAAATCGTC 
AAGACCAAAC AACGCCGGAT CTTCATGCTG AATGACGTGC TCATGTGTGC CACCGCCAGC 
TCACGGAACT CACATGAAAG CCATGCCGTG ATGAGCCAGA GGTACCTGCT CAAGTGGAGT 
GTCCCTCTAG GTCACGTGGA TGTGATTCAG TACAATGGAG GCTCTGGCGC CGGGGAGCAC 
TGTCGGCACC ACGCTGCACA CTCACCTGAG AGCCTGGCCG TGGTGGCCAA CGCGAAGCCA 
CACAAAGTTT ACATGGGGCC AGGGCAGCTG TACCAGGATC TGCAGAACCT ACTGCACGAC 
CTGAACGTTG TTGGCCAGAT CTCGCAGCTG ATAGGAAACC TCCGAGGGAG CTATCAGAAC 
TTAAACCAGT CTGTAGCCCA TGATTGGACG TCGGGCTTAC AGCGGCTGAT ACTGAGGAAA 
GAAGATGCGA TCAGAGCTGC CGACCGCTGC CGGATCCAGT TACAGCTCCC GGGAAAGCAG 
GACAAGTCCG GGCGGCCCAC GTTCTTCACT GCTGTGTTCA ATACGCTTAC ACCAGCCATC 
AAGGAGTCCT GGGTCAGCAG CCTGCAGATG GCCAAGCTGG CCCTTGAGGA GGAGAACCAT 
ATGGGATGGT TCTGTGTGGA TGACGATGGC AACCTAGCCA AGAAGGAGAC ACACCCTCTC 
CTGGTGGGAC ACATGCCTGT GATGGTGGCC AAGCAGCCAG AGTTCAAGAT TGAATGTGCA 
GCTTATAACC CTGAACCTTA CCTGAGCAAT GAAAGTCAAC CAGCCTCACC CTCCACAGCC 
CGTGGCTTTC TGTGGATTGG AAGCTGTAGC AACCAGATGG GTCAGGTCGC CATTGTCTCC 
TTCCAAGGTT CCAACCCCAA AGTCATCGAG TGCTTCAATG TGGAATCACG CATCCTGTGT 
ATGGTATACA TTCCCGCAGA GGAGTCAAAG CCCCAAGAAA CCACCGAAAC CAAGGATCCA 
GAAGCCACAG CCTCGAGAGC ACCCCATGTG CCCACCATCT GCCTGGGCAC AGAAGAGGGA 
AGCATTTCCA TTTACAAAAG CAGTCAGGGC TGCAAGAAGG TGAGGCTTCA GCACTTCTAT 
GCACCGGACA AGTCCACGGT CATGAGCTTG GCCTGCTCCC CACAAGGCCT GTATGCAGGC 
CTGGTCAATG GGTCCGTAGC CAGCTACACC AAGGCTCCAG CACCGAGCTC TCCTGGCAGC 
CCGTGGGCAG AGTTCTGTCT CATCTGCGTT TTCCTGCCAT GCTTGCCCTC GGGAGCATAT 
GCCATCTCGG GTACAGGCTG TGGTGCAGTG AAGGGCTATG GATCCTGGAA CTCGGAACCC 
CAGCAAGTGA TCAAGTTGGG TGTCCTGCCA GTCAGAAGCC TTCTCCTGGT GGAGGGTGCA 
CTGTGGGCAG CCTCGGGAGG GCAGGTGTTC ATGGCCAGCG TGGAGACTCA CACTATAGAG 
AATCAGCTGG AGGCCCACCA AGATGAAGGG ATGGTCATCT CCCACATGGC TGTGGCTGGC 
GTTGGCATCT GGATTGCCTT CACCTCGGGG TCCACCCTCC GCCTCTTCCA CACAGAGACC 
TTGAAGCACC TGCAGGATGT CAACATTGAT GCCCCTGTGC ATAGCATGCT GCCAGGGCAC 
CAGCGTCTAT CTGTGACCAG CCTGCTCGTC TGCCACGGCT TGCTCATGGT CGGTACCAGC 
CTGGGTGTCG TGGTGGCCCT GCCAGTCCCT CGGCTTCAGG GTATCCCCAA AGTGACAGGG 
CGAGGCATGG TCTCCTACCA TGCGCATAAC GGGCCCGTTA AGTTCATCGT CTCAGCCACG 
GCGTTTCAAA ACAAGGACAG AGCCAGGGAC AGCCCACGTT CCGGTTCTGA GCTTCAGGAT 
GAAGACCCCA AGGACTTGCT CTGCAGCGAG GAAGGTCCTT CCTGCCCGGG CCAGCCTGAC 
ACCTACACAT CCGTTTGGTT GGGAGACTCC CTAGGATTGC CAACTCAAAA GAATGATCTG 
TCTTCGTCCT CGGGGTCACT GAACCTGTCC CATGGCTCCA GTTCCCTAGA GCACAGATCT 
GTGGACAGCA GTCTGTGTGA CCTGCTGAGG GACCCCAGTG CCTCCCCAAG GAGCAGACCA 
CAGGGCTCTA GGCGAGCAAG AGCCAGTTCA GCGCTGGTGG TATGTGGGGG CCAGGGCCAC 
CGGCGGGTGC ACAGGAAGGC CAGGCAGCCT TCTCAGGAGG ACCTGGTTTC TTCAGTGATG 
GTGTGGCAGA TCCCCCTGCT GGGCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_017312672.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168161.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X6, mRNA.

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CloneID	OMu82410
Clone ID Related Accession (Same CDS sequence)	XM_017312672.1
Accession Version	XM_017312672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCG GTCCCTCGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG CAGTGAGCAA GGCAGCCAAG AAGGCAGTGC CCTGGCCCGA 
TGGGCAGCCG ACCCTGCCAA CACAGCATGG ATGGAAAATC CAGAGGAAGC AATTTACGAC 
GACGTTCCGA GGGAAAACTC GGACTCTGAG CCAGATGAAA TGATTTATGA TGATGTGGAG 
AACGGCGAAG AAGGTGGAAA CAGCTCCCCA GAGTACGGGT GGAGTTCCAG CGAATTCGAA 
AGCTATGAGG AGCCAAGCGA CTCGGAGGGC AAGAACGGAA TTCCCAGGTC CTTCCTGCGC 
AGCAGTCATA AAAAGCAAAT GCAGAAGCTT ATGAAGGCAG CCAAGGAGGG CACCAAAGAT 
GGGCTGGAGA AAACCAAAGC TGCTGTGAAG AGAGGCGGCT CCTTCATCAG GACCAGGTCC 
TTGGTCTCTC AAGATCACAG GTGTTATTTT GAAGAAGAGC AGAATTTGTT CATTGACGTG 
GACTGCAAGC ACCCGGAGGC TGTCCTCACC CCAATGCCCG AGGGCTTGTC TCAGCAGCAG 
GTTGTGAGAA GATACATTCT GGGCTCCATT GTTGAGAGCG AGAAGAACTA TGTAGATGCT 
CTCCGGAGGA TTTTGGAGCA ATATGAGAAG CCGCTGTCTG AGATGGAGCC CCGGCTCCTG 
AGTGACAGGA AGCTCAGGAT GGTTTTCTAC CGCGTCAAGG AGATCCTGCA GTGTCACTCC 
ATGTTCCAGA TTGCGCTGGC CAGCCGCGTG TCCGAGTGGG ATGTCGTGGA AACAATCGGC 
GACGTTTTTG TGGCCTCGTT CTCCAAGTCC ATGGTGCTGG ATGCTTACAG CGAGTACGTG 
AACAACTTCA GCACGGCCGT GGCTGTCCTC AAGAAGACGT GTGCCACAAA GCCTGCTTTC 
CTTGAGTTCT TAAAGCTGAG CCAGGATTCC AGTCCAGACC GAGTCACACT CCACAGCCTT 
ATGATGCGGC CCATCCAGAG GTTCCCACAG TTCATCCTCC TACTGCAGGA CATGCTGAAG 
AACACCGCCA AAGGACATCC TGACAGGCTG CCCCTTCAGA TGGCGCTGAC GGAGCTCGAG 
ACCCTGGCAG AGAAGCTAAA CGAGAGAAAA AGAGATGCCG ACCAGCGATG CGAAATCAAA 
CAAATAGCAA AAGCCATAAA CGAGAGATAC CTGAACAAGC TCCTCAGCAG TGGAAACCGG 
TACCTCGTCC GGTCCGATGA CGTCATCGAG ACGGTTTACA ACGACCGGGG AGAAATCGTC 
AAGACCAAAC AACGCCGGAT CTTCATGCTG AATGACGTGC TCATGTGTGC CACCGCCAGC 
TCACGGAACT CACATGAAAG CCATGCCGTG ATGAGCCAGA GGTACCTGCT CAAGTGGAGT 
GTCCCTCTAG GTCACGTGGA TGTGATTCAG TACAATGGAG GCTCTGGCGC CGGGGAGCAC 
TGTCGGCACC ACGCTGCACA CTCACCTGAG AGCCTGGCCG TGGTGGCCAA CGCGAAGCCA 
CACAAAGTTT ACATGGGGCC AGGGCAGCTG TACCAGGATC TGCAGAACCT ACTGCACGAC 
CTGAACGTTG TTGGCCAGAT CTCGCAGCTG ATAGGAAACC TCCGAGGGAG CTATCAGAAC 
TTAAACCAGT CTGTAGCCCA TGATTGGACG TCGGGCTTAC AGCGGCTGAT ACTGAGGAAA 
GAAGATGCGA TCAGAGCTGC CGACCGCTGC CGGATCCAGT TACAGCTCCC GGGAAAGCAG 
GACAAGTCCG GGCGGCCCAC GTTCTTCACT GCTGTGTTCA ATACGCTTAC ACCAGCCATC 
AAGGAGTCCT GGGTCAGCAG CCTGCAGATG GCCAAGCTGG CCCTTGAGGA GGAGAACCAT 
ATGGGATGGT TCTGTGTGGA TGACGATGGC AACCTAGCCA AGAAGGAGAC ACACCCTCTC 
CTGGTGGGAC ACATGCCTGT GATGGTGGCC AAGCAGCCAG AGTTCAAGAT TGAATGTGCA 
GCTTATAACC CTGAACCTTA CCTGAGCAAT GAAAGTCAAC CAGCCTCACC CTCCACAGCC 
CGTGGCTTTC TGTGGATTGG AAGCTGTAGC AACCAGATGG GTCAGGTCGC CATTGTCTCC 
TTCCAAGGTT CCAACCCCAA AGTCATCGAG TGCTTCAATG TGGAATCACG CATCCTGTGT 
ATGGTATACA TTCCCGCAGA GGAGTCAAAG CCCCAAGAAA CCACCGAAAC CAAGGATCCA 
GAAGCCACAG CCTCGAGAGC ACCCCATGTG CCCACCATCT GCCTGGGCAC AGAAGAGGGA 
AGCATTTCCA TTTACAAAAG CAGTCAGGGC TGCAAGAAGG TGAGGCTTCA GCACTTCTAT 
GCACCGGACA AGTCCACGGT CATGAGCTTG GCCTGCTCCC CACAAGGCCT GTATGCAGGC 
CTGGTCAATG GGTCCGTAGC CAGCTACACC AAGGCTCCAG ATGGATCCTG GAACTCGGAA 
CCCCAGCAAG TGATCAAGTT GGGTGTCCTG CCAGTCAGAA GCCTTCTCCT GGTGGAGGGT 
GCACTGTGGG CAGCCTCGGG AGGGCAGGTG TTCATGGCCA GCGTGGAGAC TCACACTATA 
GAGAATCAGC TGGAGGCCCA CCAAGATGAA GGGATGGTCA TCTCCCACAT GGCTGTGGCT 
GGCGTTGGCA TCTGGATTGC CTTCACCTCG GGGTCCACCC TCCGCCTCTT CCACACAGAG 
ACCTTGAAGC ACCTGCAGGA TGTCAACATT GATGCCCCTG TGCATAGCAT GCTGCCAGGG 
CACCAGCGTC TATCTGTGAC CAGCCTGCTC GTCTGCCACG GCTTGCTCAT GGTCGGTACC 
AGCCTGGGTG TCGTGGTGGC CCTGCCAGTC CCTCGGCTTC AGGGTATCCC CAAAGTGACA 
GGGCGAGGCA TGGTCTCCTA CCATGCGCAT AACGGGCCCG TTAAGTTCAT CGTCTCAGCC 
ACGGCGTTTC AAAACAAGGA CAGAGCCAGG GACAGCCCAC GTTCCGGTTC TGAGCTTCAG 
GATGAAGACC CCAAGGACTT GCTCTGCAGC GAGGAAGGTC CTTCCTGCCC GGGCCAGCCT 
GACACCTACA CATCCGTTTG GTTGGGAGAC TCCCTAGGAT TGCCAACTCA AAAGAATGAT 
CTGTCTTCGT CCTCGGGGTC ACTGAACCTG TCCCATGGCT CCAGTTCCCT AGAGCACAGA 
TCTGTGGACA GCAGTCTGTG TGACCTGCTG AGGGACCCCA GTGCCTCCCC AAGGAGCAGA 
CCACAGGGCT CTAGGCGAGC AAGAGCCAGT TCAGCGCTGG TGGTATGTGG GGGCCAGGGC 
CACCGGCGGG TGCACAGGAA GGCCAGGCAG CCTTCTCAGG AGGACCTGGT TTCTTCAGTG 
ATGGTGTGGC AGATCCCCCT GCTGGGCATG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168161.1
CDS	77..4069
Translation	MAPGPSGPLSPDAVQAWRKEARLSMEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPDGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_017312672.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312672.1

ORF Insert Sequence:

ATGGCGCCCG GTCCCTCGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG CAGTGAGCAA GGCAGCCAAG AAGGCAGTGC CCTGGCCCGA 
TGGGCAGCCG ACCCTGCCAA CACAGCATGG ATGGAAAATC CAGAGGAAGC AATTTACGAC 
GACGTTCCGA GGGAAAACTC GGACTCTGAG CCAGATGAAA TGATTTATGA TGATGTGGAG 
AACGGCGAAG AAGGTGGAAA CAGCTCCCCA GAGTACGGGT GGAGTTCCAG CGAATTCGAA 
AGCTATGAGG AGCCAAGCGA CTCGGAGGGC AAGAACGGAA TTCCCAGGTC CTTCCTGCGC 
AGCAGTCATA AAAAGCAAAT GCAGAAGCTT ATGAAGGCAG CCAAGGAGGG CACCAAAGAT 
GGGCTGGAGA AAACCAAAGC TGCTGTGAAG AGAGGCGGCT CCTTCATCAG GACCAGGTCC 
TTGGTCTCTC AAGATCACAG GTGTTATTTT GAAGAAGAGC AGAATTTGTT CATTGACGTG 
GACTGCAAGC ACCCGGAGGC TGTCCTCACC CCAATGCCCG AGGGCTTGTC TCAGCAGCAG 
GTTGTGAGAA GATACATTCT GGGCTCCATT GTTGAGAGCG AGAAGAACTA TGTAGATGCT 
CTCCGGAGGA TTTTGGAGCA ATATGAGAAG CCGCTGTCTG AGATGGAGCC CCGGCTCCTG 
AGTGACAGGA AGCTCAGGAT GGTTTTCTAC CGCGTCAAGG AGATCCTGCA GTGTCACTCC 
ATGTTCCAGA TTGCGCTGGC CAGCCGCGTG TCCGAGTGGG ATGTCGTGGA AACAATCGGC 
GACGTTTTTG TGGCCTCGTT CTCCAAGTCC ATGGTGCTGG ATGCTTACAG CGAGTACGTG 
AACAACTTCA GCACGGCCGT GGCTGTCCTC AAGAAGACGT GTGCCACAAA GCCTGCTTTC 
CTTGAGTTCT TAAAGCTGAG CCAGGATTCC AGTCCAGACC GAGTCACACT CCACAGCCTT 
ATGATGCGGC CCATCCAGAG GTTCCCACAG TTCATCCTCC TACTGCAGGA CATGCTGAAG 
AACACCGCCA AAGGACATCC TGACAGGCTG CCCCTTCAGA TGGCGCTGAC GGAGCTCGAG 
ACCCTGGCAG AGAAGCTAAA CGAGAGAAAA AGAGATGCCG ACCAGCGATG CGAAATCAAA 
CAAATAGCAA AAGCCATAAA CGAGAGATAC CTGAACAAGC TCCTCAGCAG TGGAAACCGG 
TACCTCGTCC GGTCCGATGA CGTCATCGAG ACGGTTTACA ACGACCGGGG AGAAATCGTC 
AAGACCAAAC AACGCCGGAT CTTCATGCTG AATGACGTGC TCATGTGTGC CACCGCCAGC 
TCACGGAACT CACATGAAAG CCATGCCGTG ATGAGCCAGA GGTACCTGCT CAAGTGGAGT 
GTCCCTCTAG GTCACGTGGA TGTGATTCAG TACAATGGAG GCTCTGGCGC CGGGGAGCAC 
TGTCGGCACC ACGCTGCACA CTCACCTGAG AGCCTGGCCG TGGTGGCCAA CGCGAAGCCA 
CACAAAGTTT ACATGGGGCC AGGGCAGCTG TACCAGGATC TGCAGAACCT ACTGCACGAC 
CTGAACGTTG TTGGCCAGAT CTCGCAGCTG ATAGGAAACC TCCGAGGGAG CTATCAGAAC 
TTAAACCAGT CTGTAGCCCA TGATTGGACG TCGGGCTTAC AGCGGCTGAT ACTGAGGAAA 
GAAGATGCGA TCAGAGCTGC CGACCGCTGC CGGATCCAGT TACAGCTCCC GGGAAAGCAG 
GACAAGTCCG GGCGGCCCAC GTTCTTCACT GCTGTGTTCA ATACGCTTAC ACCAGCCATC 
AAGGAGTCCT GGGTCAGCAG CCTGCAGATG GCCAAGCTGG CCCTTGAGGA GGAGAACCAT 
ATGGGATGGT TCTGTGTGGA TGACGATGGC AACCTAGCCA AGAAGGAGAC ACACCCTCTC 
CTGGTGGGAC ACATGCCTGT GATGGTGGCC AAGCAGCCAG AGTTCAAGAT TGAATGTGCA 
GCTTATAACC CTGAACCTTA CCTGAGCAAT GAAAGTCAAC CAGCCTCACC CTCCACAGCC 
CGTGGCTTTC TGTGGATTGG AAGCTGTAGC AACCAGATGG GTCAGGTCGC CATTGTCTCC 
TTCCAAGGTT CCAACCCCAA AGTCATCGAG TGCTTCAATG TGGAATCACG CATCCTGTGT 
ATGGTATACA TTCCCGCAGA GGAGTCAAAG CCCCAAGAAA CCACCGAAAC CAAGGATCCA 
GAAGCCACAG CCTCGAGAGC ACCCCATGTG CCCACCATCT GCCTGGGCAC AGAAGAGGGA 
AGCATTTCCA TTTACAAAAG CAGTCAGGGC TGCAAGAAGG TGAGGCTTCA GCACTTCTAT 
GCACCGGACA AGTCCACGGT CATGAGCTTG GCCTGCTCCC CACAAGGCCT GTATGCAGGC 
CTGGTCAATG GGTCCGTAGC CAGCTACACC AAGGCTCCAG ATGGATCCTG GAACTCGGAA 
CCCCAGCAAG TGATCAAGTT GGGTGTCCTG CCAGTCAGAA GCCTTCTCCT GGTGGAGGGT 
GCACTGTGGG CAGCCTCGGG AGGGCAGGTG TTCATGGCCA GCGTGGAGAC TCACACTATA 
GAGAATCAGC TGGAGGCCCA CCAAGATGAA GGGATGGTCA TCTCCCACAT GGCTGTGGCT 
GGCGTTGGCA TCTGGATTGC CTTCACCTCG GGGTCCACCC TCCGCCTCTT CCACACAGAG 
ACCTTGAAGC ACCTGCAGGA TGTCAACATT GATGCCCCTG TGCATAGCAT GCTGCCAGGG 
CACCAGCGTC TATCTGTGAC CAGCCTGCTC GTCTGCCACG GCTTGCTCAT GGTCGGTACC 
AGCCTGGGTG TCGTGGTGGC CCTGCCAGTC CCTCGGCTTC AGGGTATCCC CAAAGTGACA 
GGGCGAGGCA TGGTCTCCTA CCATGCGCAT AACGGGCCCG TTAAGTTCAT CGTCTCAGCC 
ACGGCGTTTC AAAACAAGGA CAGAGCCAGG GACAGCCCAC GTTCCGGTTC TGAGCTTCAG 
GATGAAGACC CCAAGGACTT GCTCTGCAGC GAGGAAGGTC CTTCCTGCCC GGGCCAGCCT 
GACACCTACA CATCCGTTTG GTTGGGAGAC TCCCTAGGAT TGCCAACTCA AAAGAATGAT 
CTGTCTTCGT CCTCGGGGTC ACTGAACCTG TCCCATGGCT CCAGTTCCCT AGAGCACAGA 
TCTGTGGACA GCAGTCTGTG TGACCTGCTG AGGGACCCCA GTGCCTCCCC AAGGAGCAGA 
CCACAGGGCT CTAGGCGAGC AAGAGCCAGT TCAGCGCTGG TGGTATGTGG GGGCCAGGGC 
CACCGGCGGG TGCACAGGAA GGCCAGGCAG CCTTCTCAGG AGGACCTGGT TTCTTCAGTG 
ATGGTGTGGC AGATCCCCCT GCTGGGCATG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( NM_001037736.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001032825.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant 2, mRNA.

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CloneID	OMu12832
Clone ID Related Accession (Same CDS sequence)	NM_001037736.2
Accession Version	NM_001037736.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147669.1, BC066074.1, BC059212.1 and CD774578.1. On Nov 6, 2013 this sequence version replaced NM_001037736.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This encodes isoform b, which is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: BC066074.1, SRR1660819.113468.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAAATGC AGAAGCTTAT GAAGGCAGCC AAGGAGGGCA CCAAAGATGG GCTGGAGAAA 
ACCAAAGCTG CTGTGAAGAG AGGCGGCTCC TTCATCAGGA CCAGGTCCTT GGTCTCTCAA 
GATCACAGGT GTTATTTTGA AGAAGAGCAG AATTTGTTCA TTGACGTGGA CTGCAAGCAC 
CCGGAGGCTG TCCTCACCCC AATGCCCGAG GGCTTGTCTC AGCAGCAGGT TGTGAGAAGA 
TACATTCTGG GCTCCATTGT TGAGAGCGAG AAGAACTATG TAGATGCTCT CCGGAGGATT 
TTGGAGCAAT ATGAGAAGCC GCTGTCTGAG ATGGAGCCCC GGCTCCTGAG TGACAGGAAG 
CTCAGGATGG TTTTCTACCG CGTCAAGGAG ATCCTGCAGT GTCACTCCAT GTTCCAGATT 
GCGCTGGCCA GCCGCGTGTC CGAGTGGGAT GTCGTGGAAA CAATCGGCGA CGTTTTTGTG 
GCCTCGTTCT CCAAGTCCAT GGTGCTGGAT GCTTACAGCG AGTACGTGAA CAACTTCAGC 
ACGGCCGTGG CTGTCCTCAA GAAGACGTGT GCCACAAAGC CTGCTTTCCT TGAGTTCTTA 
AAGCTGAGCC AGGATTCCAG TCCAGACCGA GTCACACTCC ACAGCCTTAT GATGCGGCCC 
ATCCAGAGGT TCCCACAGTT CATCCTCCTA CTGCAGGACA TGCTGAAGAA CACCGCCAAA 
GGACATCCTG ACAGGCTGCC CCTTCAGATG GCGCTGACGG AGCTCGAGAC CCTGGCAGAG 
AAGCTAAACG AGAGAAAAAG AGATGCCGAC CAGCGATGCG AAATCAAACA AATAGCAAAA 
GCCATAAACG AGAGATACCT GAACAAGCTC CTCAGCAGTG GAAACCGGTA CCTCGTCCGG 
TCCGATGACG TCATCGAGAC GGTTTACAAC GACCGGGGAG AAATCGTCAA GACCAAACAA 
CGCCGGATCT TCATGCTGAA TGACGTGCTC ATGTGTGCCA CCGCCAGCTC ACGGAACTCA 
CATGAAAGCC ATGCCGTGAT GAGCCAGAGG TACCTGCTCA AGTGGAGTGT CCCTCTAGGT 
CACGTGGATG TGATTCAGTA CAATGGAGGC TCTGGCGCCG GGGAGCACTG TCGGCACCAC 
GCTGCACACT CACCTGAGAG CCTGGCCGTG GTGGCCAACG CGAAGCCACA CAAAGTTTAC 
ATGGGGCCAG GGCAGCTGTA CCAGGATCTG CAGAACCTAC TGCACGACCT GAACGTTGTT 
GGCCAGATCT CGCAGCTGAT AGGAAACCTC CGAGGGAGCT ATCAGAACTT AAACCAGTCT 
GTAGCCCATG ATTGGACGTC GGGCTTACAG CGGCTGATAC TGAGGAAAGA AGATGCGATC 
AGAGCTGCCG ACCGCTGCCG GATCCAGTTA CAGCTCCCGG GAAAGCAGGA CAAGTCCGGG 
CGGCCCACGT TCTTCACTGC TGTGTTCAAT ACGCTTACAC CAGCCATCAA GGAGTCCTGG 
GTCAGCAGCC TGCAGATGGC CAAGCTGGCC CTTGAGGAGG AGAACCATAT GGGATGGTTC 
TGTGTGGATG ACGATGGCAA CCTAGCCAAG AAGGAGACAC ACCCTCTCCT GGTGGGACAC 
ATGCCTGTGA TGGTGGCCAA GCAGCCAGAG TTCAAGATTG AATGTGCAGC TTATAACCCT 
GAACCTTACC TGAGCAATGA AAGTCAACCA GCCTCACCCT CCACAGCCCG TGGCTTTCTG 
TGGATTGGAA GCTGTAGCAA CCAGATGGGT CAGGTCGCCA TTGTCTCCTT CCAAGGTTCC 
AACCCCAAAG TCATCGAGTG CTTCAATGTG GAATCACGCA TCCTGTGTAT GGTATACATT 
CCCGCAGAGG AGTCAAAGCC CCAAGAAACC ACCGAAACCA AGGATCCAGA AGCCACAGCC 
TCGAGAGCAC CCCATGTGCC CACCATCTGC CTGGGCACAG AAGAGGGAAG CATTTCCATT 
TACAAAAGCA GTCAGGGCTG CAAGAAGGTG AGGCTTCAGC ACTTCTATGC ACCGGACAAG 
TCCACGGTCA TGAGCTTGGC CTGCTCCCCA CAAGGCCTGT ATGCAGGCCT GGTCAATGGG 
TCCGTAGCCA GCTACACCAA GGCTCCAGAT GGATCCTGGA ACTCGGAACC CCAGCAAGTG 
ATCAAGTTGG GTGTCCTGCC AGTCAGAAGC CTTCTCCTGG TGGAGGGTGC ACTGTGGGCA 
GCCTCGGGAG GGCAGGTGTT CATGGCCAGC GTGGAGACTC ACACTATAGA GAATCAGCTG 
GAGGCCCACC AAGATGAAGG GATGGTCATC TCCCACATGG CTGTGGCTGG CGTTGGCATC 
TGGATTGCCT TCACCTCGGG GTCCACCCTC CGCCTCTTCC ACACAGAGAC CTTGAAGCAC 
CTGCAGGATG TCAACATTGA TGCCCCTGTG CATAGCATGC TGCCAGGGCA CCAGCGTCTA 
TCTGTGACCA GCCTGCTCGT CTGCCACGGC TTGCTCATGG TCGGTACCAG CCTGGGTGTC 
GTGGTGGCCC TGCCAGTCCC TCGGCTTCAG GGTATCCCCA AAGTGACAGG GCGAGGCATG 
GTCTCCTACC ATGCGCATAA CGGGCCCGTT AAGTTCATCG TCTCAGCCAC GGCGTTTCAA 
AACAAGGACA GAGCCAGGGA CAGCCCACGT TCCGGTTCTG AGCTTCAGGA TGAAGACCCC 
AAGGACTTGC TCTGCAGCGA GGAAGGTCCT TCCTGCCCGG GCCAGCCTGA CACCTACACA 
TCCGTTTGGT TGGGAGACTC CCTAGGATTG CCAACTCAAA AGAATGATCT GTCTTCGTCC 
TCGGGGTCAC TGAACCTGTC CCATGGCTCC AGTTCCCTAG AGCACAGATC TGTGGACAGC 
AGTCTGTGTG ACCTGCTGAG GGACCCCAGT GCCTCCCCAA GGAGCAGACC ACAGGGCTCT 
AGGCGAGCAA GAGCCAGTTC AGCGCTGGTG GTATGTGGGG GCCAGGGCCA CCGGCGGGTG 
CACAGGAAGG CCAGGCAGCC TTCTCAGGAG GACCTGGTTT CTTCAGTGAT GGTGTGGCAG 
ATCCCCCTGC TGGGCATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032825.1
CDS	236..4156
Translation	MEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPDGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	NM_001037736.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037736.2

ORF Insert Sequence:

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAAATGC AGAAGCTTAT GAAGGCAGCC AAGGAGGGCA CCAAAGATGG GCTGGAGAAA 
ACCAAAGCTG CTGTGAAGAG AGGCGGCTCC TTCATCAGGA CCAGGTCCTT GGTCTCTCAA 
GATCACAGGT GTTATTTTGA AGAAGAGCAG AATTTGTTCA TTGACGTGGA CTGCAAGCAC 
CCGGAGGCTG TCCTCACCCC AATGCCCGAG GGCTTGTCTC AGCAGCAGGT TGTGAGAAGA 
TACATTCTGG GCTCCATTGT TGAGAGCGAG AAGAACTATG TAGATGCTCT CCGGAGGATT 
TTGGAGCAAT ATGAGAAGCC GCTGTCTGAG ATGGAGCCCC GGCTCCTGAG TGACAGGAAG 
CTCAGGATGG TTTTCTACCG CGTCAAGGAG ATCCTGCAGT GTCACTCCAT GTTCCAGATT 
GCGCTGGCCA GCCGCGTGTC CGAGTGGGAT GTCGTGGAAA CAATCGGCGA CGTTTTTGTG 
GCCTCGTTCT CCAAGTCCAT GGTGCTGGAT GCTTACAGCG AGTACGTGAA CAACTTCAGC 
ACGGCCGTGG CTGTCCTCAA GAAGACGTGT GCCACAAAGC CTGCTTTCCT TGAGTTCTTA 
AAGCTGAGCC AGGATTCCAG TCCAGACCGA GTCACACTCC ACAGCCTTAT GATGCGGCCC 
ATCCAGAGGT TCCCACAGTT CATCCTCCTA CTGCAGGACA TGCTGAAGAA CACCGCCAAA 
GGACATCCTG ACAGGCTGCC CCTTCAGATG GCGCTGACGG AGCTCGAGAC CCTGGCAGAG 
AAGCTAAACG AGAGAAAAAG AGATGCCGAC CAGCGATGCG AAATCAAACA AATAGCAAAA 
GCCATAAACG AGAGATACCT GAACAAGCTC CTCAGCAGTG GAAACCGGTA CCTCGTCCGG 
TCCGATGACG TCATCGAGAC GGTTTACAAC GACCGGGGAG AAATCGTCAA GACCAAACAA 
CGCCGGATCT TCATGCTGAA TGACGTGCTC ATGTGTGCCA CCGCCAGCTC ACGGAACTCA 
CATGAAAGCC ATGCCGTGAT GAGCCAGAGG TACCTGCTCA AGTGGAGTGT CCCTCTAGGT 
CACGTGGATG TGATTCAGTA CAATGGAGGC TCTGGCGCCG GGGAGCACTG TCGGCACCAC 
GCTGCACACT CACCTGAGAG CCTGGCCGTG GTGGCCAACG CGAAGCCACA CAAAGTTTAC 
ATGGGGCCAG GGCAGCTGTA CCAGGATCTG CAGAACCTAC TGCACGACCT GAACGTTGTT 
GGCCAGATCT CGCAGCTGAT AGGAAACCTC CGAGGGAGCT ATCAGAACTT AAACCAGTCT 
GTAGCCCATG ATTGGACGTC GGGCTTACAG CGGCTGATAC TGAGGAAAGA AGATGCGATC 
AGAGCTGCCG ACCGCTGCCG GATCCAGTTA CAGCTCCCGG GAAAGCAGGA CAAGTCCGGG 
CGGCCCACGT TCTTCACTGC TGTGTTCAAT ACGCTTACAC CAGCCATCAA GGAGTCCTGG 
GTCAGCAGCC TGCAGATGGC CAAGCTGGCC CTTGAGGAGG AGAACCATAT GGGATGGTTC 
TGTGTGGATG ACGATGGCAA CCTAGCCAAG AAGGAGACAC ACCCTCTCCT GGTGGGACAC 
ATGCCTGTGA TGGTGGCCAA GCAGCCAGAG TTCAAGATTG AATGTGCAGC TTATAACCCT 
GAACCTTACC TGAGCAATGA AAGTCAACCA GCCTCACCCT CCACAGCCCG TGGCTTTCTG 
TGGATTGGAA GCTGTAGCAA CCAGATGGGT CAGGTCGCCA TTGTCTCCTT CCAAGGTTCC 
AACCCCAAAG TCATCGAGTG CTTCAATGTG GAATCACGCA TCCTGTGTAT GGTATACATT 
CCCGCAGAGG AGTCAAAGCC CCAAGAAACC ACCGAAACCA AGGATCCAGA AGCCACAGCC 
TCGAGAGCAC CCCATGTGCC CACCATCTGC CTGGGCACAG AAGAGGGAAG CATTTCCATT 
TACAAAAGCA GTCAGGGCTG CAAGAAGGTG AGGCTTCAGC ACTTCTATGC ACCGGACAAG 
TCCACGGTCA TGAGCTTGGC CTGCTCCCCA CAAGGCCTGT ATGCAGGCCT GGTCAATGGG 
TCCGTAGCCA GCTACACCAA GGCTCCAGAT GGATCCTGGA ACTCGGAACC CCAGCAAGTG 
ATCAAGTTGG GTGTCCTGCC AGTCAGAAGC CTTCTCCTGG TGGAGGGTGC ACTGTGGGCA 
GCCTCGGGAG GGCAGGTGTT CATGGCCAGC GTGGAGACTC ACACTATAGA GAATCAGCTG 
GAGGCCCACC AAGATGAAGG GATGGTCATC TCCCACATGG CTGTGGCTGG CGTTGGCATC 
TGGATTGCCT TCACCTCGGG GTCCACCCTC CGCCTCTTCC ACACAGAGAC CTTGAAGCAC 
CTGCAGGATG TCAACATTGA TGCCCCTGTG CATAGCATGC TGCCAGGGCA CCAGCGTCTA 
TCTGTGACCA GCCTGCTCGT CTGCCACGGC TTGCTCATGG TCGGTACCAG CCTGGGTGTC 
GTGGTGGCCC TGCCAGTCCC TCGGCTTCAG GGTATCCCCA AAGTGACAGG GCGAGGCATG 
GTCTCCTACC ATGCGCATAA CGGGCCCGTT AAGTTCATCG TCTCAGCCAC GGCGTTTCAA 
AACAAGGACA GAGCCAGGGA CAGCCCACGT TCCGGTTCTG AGCTTCAGGA TGAAGACCCC 
AAGGACTTGC TCTGCAGCGA GGAAGGTCCT TCCTGCCCGG GCCAGCCTGA CACCTACACA 
TCCGTTTGGT TGGGAGACTC CCTAGGATTG CCAACTCAAA AGAATGATCT GTCTTCGTCC 
TCGGGGTCAC TGAACCTGTC CCATGGCTCC AGTTCCCTAG AGCACAGATC TGTGGACAGC 
AGTCTGTGTG ACCTGCTGAG GGACCCCAGT GCCTCCCCAA GGAGCAGACC ACAGGGCTCT 
AGGCGAGCAA GAGCCAGTTC AGCGCTGGTG GTATGTGGGG GCCAGGGCCA CCGGCGGGTG 
CACAGGAAGG CCAGGCAGCC TTCTCAGGAG GACCTGGTTT CTTCAGTGAT GGTGTGGCAG 
ATCCCCCTGC TGGGCATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( NM_172751.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766339.2 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu03998
Clone ID Related Accession (Same CDS sequence)	NM_172751.3
Accession Version	NM_172751.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147669.1, BC059212.1, BC066074.1 and CD774578.1. On Nov 6, 2013 this sequence version replaced NM_172751.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform a). ##Evidence-Data-START## Transcript exon combination :: AK147669.1, SRR1660815.130998.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAACTCT CTCATGACCT AACCCGCTTT AAGGCGCACT GTGAGGAAAA GATGAGAGGC 
TTGGTGGCCA GTACGGTGGG AGCCATGGAG ATTCAGCAGG CAAAGCAGAG GCAGGAGCGG 
AAGATGCAGA AGCTTATGAA GGCAGCCAAG GAGGGCACCA AAGATGGGCT GGAGAAAACC 
AAAGCTGCTG TGAAGAGAGG CGGCTCCTTC ATCAGGACCA GGTCCTTGGT CTCTCAAGAT 
CACAGGTGTT ATTTTGAAGA AGAGCAGAAT TTGTTCATTG ACGTGGACTG CAAGCACCCG 
GAGGCTGTCC TCACCCCAAT GCCCGAGGGC TTGTCTCAGC AGCAGGTTGT GAGAAGATAC 
ATTCTGGGCT CCATTGTTGA GAGCGAGAAG AACTATGTAG ATGCTCTCCG GAGGATTTTG 
GAGCAATATG AGAAGCCGCT GTCTGAGATG GAGCCCCGGC TCCTGAGTGA CAGGAAGCTC 
AGGATGGTTT TCTACCGCGT CAAGGAGATC CTGCAGTGTC ACTCCATGTT CCAGATTGCG 
CTGGCCAGCC GCGTGTCCGA GTGGGATGTC GTGGAAACAA TCGGCGACGT TTTTGTGGCC 
TCGTTCTCCA AGTCCATGGT GCTGGATGCT TACAGCGAGT ACGTGAACAA CTTCAGCACG 
GCCGTGGCTG TCCTCAAGAA GACGTGTGCC ACAAAGCCTG CTTTCCTTGA GTTCTTAAAG 
CTGAGCCAGG ATTCCAGTCC AGACCGAGTC ACACTCCACA GCCTTATGAT GCGGCCCATC 
CAGAGGTTCC CACAGTTCAT CCTCCTACTG CAGGACATGC TGAAGAACAC CGCCAAAGGA 
CATCCTGACA GGCTGCCCCT TCAGATGGCG CTGACGGAGC TCGAGACCCT GGCAGAGAAG 
CTAAACGAGA GAAAAAGAGA TGCCGACCAG CGATGCGAAA TCAAACAAAT AGCAAAAGCC 
ATAAACGAGA GATACCTGAA CAAGCTCCTC AGCAGTGGAA ACCGGTACCT CGTCCGGTCC 
GATGACGTCA TCGAGACGGT TTACAACGAC CGGGGAGAAA TCGTCAAGAC CAAACAACGC 
CGGATCTTCA TGCTGAATGA CGTGCTCATG TGTGCCACCG CCAGCTCACG GAACTCACAT 
GAAAGCCATG CCGTGATGAG CCAGAGGTAC CTGCTCAAGT GGAGTGTCCC TCTAGGTCAC 
GTGGATGTGA TTCAGTACAA TGGAGGCTCT GGCGCCGGGG AGCACTGTCG GCACCACGCT 
GCACACTCAC CTGAGAGCCT GGCCGTGGTG GCCAACGCGA AGCCACACAA AGTTTACATG 
GGGCCAGGGC AGCTGTACCA GGATCTGCAG AACCTACTGC ACGACCTGAA CGTTGTTGGC 
CAGATCTCGC AGCTGATAGG AAACCTCCGA GGGAGCTATC AGAACTTAAA CCAGTCTGTA 
GCCCATGATT GGACGTCGGG CTTACAGCGG CTGATACTGA GGAAAGAAGA TGCGATCAGA 
GCTGCCGACC GCTGCCGGAT CCAGTTACAG CTCCCGGGAA AGCAGGACAA GTCCGGGCGG 
CCCACGTTCT TCACTGCTGT GTTCAATACG CTTACACCAG CCATCAAGGA GTCCTGGGTC 
AGCAGCCTGC AGATGGCCAA GCTGGCCCTT GAGGAGGAGA ACCATATGGG ATGGTTCTGT 
GTGGATGACG ATGGCAACCT AGCCAAGAAG GAGACACACC CTCTCCTGGT GGGACACATG 
CCTGTGATGG TGGCCAAGCA GCCAGAGTTC AAGATTGAAT GTGCAGCTTA TAACCCTGAA 
CCTTACCTGA GCAATGAAAG TCAACCAGCC TCACCCTCCA CAGCCCGTGG CTTTCTGTGG 
ATTGGAAGCT GTAGCAACCA GATGGGTCAG GTCGCCATTG TCTCCTTCCA AGGTTCCAAC 
CCCAAAGTCA TCGAGTGCTT CAATGTGGAA TCACGCATCC TGTGTATGGT ATACATTCCC 
GCAGAGGAGT CAAAGCCCCA AGAAACCACC GAAACCAAGG ATCCAGAAGC CACAGCCTCG 
AGAGCACCCC ATGTGCCCAC CATCTGCCTG GGCACAGAAG AGGGAAGCAT TTCCATTTAC 
AAAAGCAGTC AGGGCTGCAA GAAGGTGAGG CTTCAGCACT TCTATGCACC GGACAAGTCC 
ACGGTCATGA GCTTGGCCTG CTCCCCACAA GGCCTGTATG CAGGCCTGGT CAATGGGTCC 
GTAGCCAGCT ACACCAAGGC TCCAGATGGA TCCTGGAACT CGGAACCCCA GCAAGTGATC 
AAGTTGGGTG TCCTGCCAGT CAGAAGCCTT CTCCTGGTGG AGGGTGCACT GTGGGCAGCC 
TCGGGAGGGC AGGTGTTCAT GGCCAGCGTG GAGACTCACA CTATAGAGAA TCAGCTGGAG 
GCCCACCAAG ATGAAGGGAT GGTCATCTCC CACATGGCTG TGGCTGGCGT TGGCATCTGG 
ATTGCCTTCA CCTCGGGGTC CACCCTCCGC CTCTTCCACA CAGAGACCTT GAAGCACCTG 
CAGGATGTCA ACATTGATGC CCCTGTGCAT AGCATGCTGC CAGGGCACCA GCGTCTATCT 
GTGACCAGCC TGCTCGTCTG CCACGGCTTG CTCATGGTCG GTACCAGCCT GGGTGTCGTG 
GTGGCCCTGC CAGTCCCTCG GCTTCAGGGT ATCCCCAAAG TGACAGGGCG AGGCATGGTC 
TCCTACCATG CGCATAACGG GCCCGTTAAG TTCATCGTCT CAGCCACGGC GTTTCAAAAC 
AAGGACAGAG CCAGGGACAG CCCACGTTCC GGTTCTGAGC TTCAGGATGA AGACCCCAAG 
GACTTGCTCT GCAGCGAGGA AGGTCCTTCC TGCCCGGGCC AGCCTGACAC CTACACATCC 
GTTTGGTTGG GAGACTCCCT AGGATTGCCA ACTCAAAAGA ATGATCTGTC TTCGTCCTCG 
GGGTCACTGA ACCTGTCCCA TGGCTCCAGT TCCCTAGAGC ACAGATCTGT GGACAGCAGT 
CTGTGTGACC TGCTGAGGGA CCCCAGTGCC TCCCCAAGGA GCAGACCACA GGGCTCTAGG 
CGAGCAAGAG CCAGTTCAGC GCTGGTGGTA TGTGGGGGCC AGGGCCACCG GCGGGTGCAC 
AGGAAGGCCA GGCAGCCTTC TCAGGAGGAC CTGGTTTCTT CAGTGATGGT GTGGCAGATC 
CCCCTGCTGG GCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766339.2
CDS	236..4273
Translation	MEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQLSHDLTRFKAHCEEKMRGLVASTVGAMEIQQAKQRQERKMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPDGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	NM_172751.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172751.3

ORF Insert Sequence:

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAACTCT CTCATGACCT AACCCGCTTT AAGGCGCACT GTGAGGAAAA GATGAGAGGC 
TTGGTGGCCA GTACGGTGGG AGCCATGGAG ATTCAGCAGG CAAAGCAGAG GCAGGAGCGG 
AAGATGCAGA AGCTTATGAA GGCAGCCAAG GAGGGCACCA AAGATGGGCT GGAGAAAACC 
AAAGCTGCTG TGAAGAGAGG CGGCTCCTTC ATCAGGACCA GGTCCTTGGT CTCTCAAGAT 
CACAGGTGTT ATTTTGAAGA AGAGCAGAAT TTGTTCATTG ACGTGGACTG CAAGCACCCG 
GAGGCTGTCC TCACCCCAAT GCCCGAGGGC TTGTCTCAGC AGCAGGTTGT GAGAAGATAC 
ATTCTGGGCT CCATTGTTGA GAGCGAGAAG AACTATGTAG ATGCTCTCCG GAGGATTTTG 
GAGCAATATG AGAAGCCGCT GTCTGAGATG GAGCCCCGGC TCCTGAGTGA CAGGAAGCTC 
AGGATGGTTT TCTACCGCGT CAAGGAGATC CTGCAGTGTC ACTCCATGTT CCAGATTGCG 
CTGGCCAGCC GCGTGTCCGA GTGGGATGTC GTGGAAACAA TCGGCGACGT TTTTGTGGCC 
TCGTTCTCCA AGTCCATGGT GCTGGATGCT TACAGCGAGT ACGTGAACAA CTTCAGCACG 
GCCGTGGCTG TCCTCAAGAA GACGTGTGCC ACAAAGCCTG CTTTCCTTGA GTTCTTAAAG 
CTGAGCCAGG ATTCCAGTCC AGACCGAGTC ACACTCCACA GCCTTATGAT GCGGCCCATC 
CAGAGGTTCC CACAGTTCAT CCTCCTACTG CAGGACATGC TGAAGAACAC CGCCAAAGGA 
CATCCTGACA GGCTGCCCCT TCAGATGGCG CTGACGGAGC TCGAGACCCT GGCAGAGAAG 
CTAAACGAGA GAAAAAGAGA TGCCGACCAG CGATGCGAAA TCAAACAAAT AGCAAAAGCC 
ATAAACGAGA GATACCTGAA CAAGCTCCTC AGCAGTGGAA ACCGGTACCT CGTCCGGTCC 
GATGACGTCA TCGAGACGGT TTACAACGAC CGGGGAGAAA TCGTCAAGAC CAAACAACGC 
CGGATCTTCA TGCTGAATGA CGTGCTCATG TGTGCCACCG CCAGCTCACG GAACTCACAT 
GAAAGCCATG CCGTGATGAG CCAGAGGTAC CTGCTCAAGT GGAGTGTCCC TCTAGGTCAC 
GTGGATGTGA TTCAGTACAA TGGAGGCTCT GGCGCCGGGG AGCACTGTCG GCACCACGCT 
GCACACTCAC CTGAGAGCCT GGCCGTGGTG GCCAACGCGA AGCCACACAA AGTTTACATG 
GGGCCAGGGC AGCTGTACCA GGATCTGCAG AACCTACTGC ACGACCTGAA CGTTGTTGGC 
CAGATCTCGC AGCTGATAGG AAACCTCCGA GGGAGCTATC AGAACTTAAA CCAGTCTGTA 
GCCCATGATT GGACGTCGGG CTTACAGCGG CTGATACTGA GGAAAGAAGA TGCGATCAGA 
GCTGCCGACC GCTGCCGGAT CCAGTTACAG CTCCCGGGAA AGCAGGACAA GTCCGGGCGG 
CCCACGTTCT TCACTGCTGT GTTCAATACG CTTACACCAG CCATCAAGGA GTCCTGGGTC 
AGCAGCCTGC AGATGGCCAA GCTGGCCCTT GAGGAGGAGA ACCATATGGG ATGGTTCTGT 
GTGGATGACG ATGGCAACCT AGCCAAGAAG GAGACACACC CTCTCCTGGT GGGACACATG 
CCTGTGATGG TGGCCAAGCA GCCAGAGTTC AAGATTGAAT GTGCAGCTTA TAACCCTGAA 
CCTTACCTGA GCAATGAAAG TCAACCAGCC TCACCCTCCA CAGCCCGTGG CTTTCTGTGG 
ATTGGAAGCT GTAGCAACCA GATGGGTCAG GTCGCCATTG TCTCCTTCCA AGGTTCCAAC 
CCCAAAGTCA TCGAGTGCTT CAATGTGGAA TCACGCATCC TGTGTATGGT ATACATTCCC 
GCAGAGGAGT CAAAGCCCCA AGAAACCACC GAAACCAAGG ATCCAGAAGC CACAGCCTCG 
AGAGCACCCC ATGTGCCCAC CATCTGCCTG GGCACAGAAG AGGGAAGCAT TTCCATTTAC 
AAAAGCAGTC AGGGCTGCAA GAAGGTGAGG CTTCAGCACT TCTATGCACC GGACAAGTCC 
ACGGTCATGA GCTTGGCCTG CTCCCCACAA GGCCTGTATG CAGGCCTGGT CAATGGGTCC 
GTAGCCAGCT ACACCAAGGC TCCAGATGGA TCCTGGAACT CGGAACCCCA GCAAGTGATC 
AAGTTGGGTG TCCTGCCAGT CAGAAGCCTT CTCCTGGTGG AGGGTGCACT GTGGGCAGCC 
TCGGGAGGGC AGGTGTTCAT GGCCAGCGTG GAGACTCACA CTATAGAGAA TCAGCTGGAG 
GCCCACCAAG ATGAAGGGAT GGTCATCTCC CACATGGCTG TGGCTGGCGT TGGCATCTGG 
ATTGCCTTCA CCTCGGGGTC CACCCTCCGC CTCTTCCACA CAGAGACCTT GAAGCACCTG 
CAGGATGTCA ACATTGATGC CCCTGTGCAT AGCATGCTGC CAGGGCACCA GCGTCTATCT 
GTGACCAGCC TGCTCGTCTG CCACGGCTTG CTCATGGTCG GTACCAGCCT GGGTGTCGTG 
GTGGCCCTGC CAGTCCCTCG GCTTCAGGGT ATCCCCAAAG TGACAGGGCG AGGCATGGTC 
TCCTACCATG CGCATAACGG GCCCGTTAAG TTCATCGTCT CAGCCACGGC GTTTCAAAAC 
AAGGACAGAG CCAGGGACAG CCCACGTTCC GGTTCTGAGC TTCAGGATGA AGACCCCAAG 
GACTTGCTCT GCAGCGAGGA AGGTCCTTCC TGCCCGGGCC AGCCTGACAC CTACACATCC 
GTTTGGTTGG GAGACTCCCT AGGATTGCCA ACTCAAAAGA ATGATCTGTC TTCGTCCTCG 
GGGTCACTGA ACCTGTCCCA TGGCTCCAGT TCCCTAGAGC ACAGATCTGT GGACAGCAGT 
CTGTGTGACC TGCTGAGGGA CCCCAGTGCC TCCCCAAGGA GCAGACCACA GGGCTCTAGG 
CGAGCAAGAG CCAGTTCAGC GCTGGTGGTA TGTGGGGGCC AGGGCCACCG GCGGGTGCAC 
AGGAAGGCCA GGCAGCCTTC TCAGGAGGAC CTGGTTTCTT CAGTGATGGT GTGGCAGATC 
CCCCTGCTGG GCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_006508768.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508831.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X1, mRNA.

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CloneID	OMu40382
Clone ID Related Accession (Same CDS sequence)	XM_006508768.3
Accession Version	XM_006508768.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508768.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCG GTCCCTCGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG CAGTGAGCAA GGCAGCCAAG AAGGCAGTGC CCTGGCCCGA 
TGGGCAGCCG ACCCTGCCAA CACAGCATGG ATGGAAAATC CAGAGGAAGC AATTTACGAC 
GACGTTCCGA GGGAAAACTC GGACTCTGAG CCAGATGAAA TGATTTATGA TGATGTGGAG 
AACGGCGAAG AAGGTGGAAA CAGCTCCCCA GAGTACGGGT GGAGTTCCAG CGAATTCGAA 
AGCTATGAGG AGCCAAGCGA CTCGGAGGGC AAGAACGGAA TTCCCAGGTC CTTCCTGCGC 
AGCAGTCATA AAAAGCAACT CTCTCATGAC CTAACCCGCT TTAAGGCGCA CTGTGAGGAA 
AAGATGAGAG GCTTGGTGGC CAGTACGGTG GGAGCCATGG AGATTCAGCA GGCAAAGCAG 
AGGCAGGAGC GGAAGATGCA GAAGCTTATG AAGGCAGCCA AGGAGGGCAC CAAAGATGGG 
CTGGAGAAAA CCAAAGCTGC TGTGAAGAGA GGCGGCTCCT TCATCAGGAC CAGGTCCTTG 
GTCTCTCAAG ATCACAGGTG TTATTTTGAA GAAGAGCAGA ATTTGTTCAT TGACGTGGAC 
TGCAAGCACC CGGAGGCTGT CCTCACCCCA ATGCCCGAGG GCTTGTCTCA GCAGCAGGTT 
GTGAGAAGAT ACATTCTGGG CTCCATTGTT GAGAGCGAGA AGAACTATGT AGATGCTCTC 
CGGAGGATTT TGGAGCAATA TGAGAAGCCG CTGTCTGAGA TGGAGCCCCG GCTCCTGAGT 
GACAGGAAGC TCAGGATGGT TTTCTACCGC GTCAAGGAGA TCCTGCAGTG TCACTCCATG 
TTCCAGATTG CGCTGGCCAG CCGCGTGTCC GAGTGGGATG TCGTGGAAAC AATCGGCGAC 
GTTTTTGTGG CCTCGTTCTC CAAGTCCATG GTGCTGGATG CTTACAGCGA GTACGTGAAC 
AACTTCAGCA CGGCCGTGGC TGTCCTCAAG AAGACGTGTG CCACAAAGCC TGCTTTCCTT 
GAGTTCTTAA AGCTGAGCCA GGATTCCAGT CCAGACCGAG TCACACTCCA CAGCCTTATG 
ATGCGGCCCA TCCAGAGGTT CCCACAGTTC ATCCTCCTAC TGCAGGACAT GCTGAAGAAC 
ACCGCCAAAG GACATCCTGA CAGGCTGCCC CTTCAGATGG CGCTGACGGA GCTCGAGACC 
CTGGCAGAGA AGCTAAACGA GAGAAAAAGA GATGCCGACC AGCGATGCGA AATCAAACAA 
ATAGCAAAAG CCATAAACGA GAGATACCTG AACAAGCTCC TCAGCAGTGG AAACCGGTAC 
CTCGTCCGGT CCGATGACGT CATCGAGACG GTTTACAACG ACCGGGGAGA AATCGTCAAG 
ACCAAACAAC GCCGGATCTT CATGCTGAAT GACGTGCTCA TGTGTGCCAC CGCCAGCTCA 
CGGAACTCAC ATGAAAGCCA TGCCGTGATG AGCCAGAGGT ACCTGCTCAA GTGGAGTGTC 
CCTCTAGGTC ACGTGGATGT GATTCAGTAC AATGGAGGCT CTGGCGCCGG GGAGCACTGT 
CGGCACCACG CTGCACACTC ACCTGAGAGC CTGGCCGTGG TGGCCAACGC GAAGCCACAC 
AAAGTTTACA TGGGGCCAGG GCAGCTGTAC CAGGATCTGC AGAACCTACT GCACGACCTG 
AACGTTGTTG GCCAGATCTC GCAGCTGATA GGAAACCTCC GAGGGAGCTA TCAGAACTTA 
AACCAGTCTG TAGCCCATGA TTGGACGTCG GGCTTACAGC GGCTGATACT GAGGAAAGAA 
GATGCGATCA GAGCTGCCGA CCGCTGCCGG ATCCAGTTAC AGCTCCCGGG AAAGCAGGAC 
AAGTCCGGGC GGCCCACGTT CTTCACTGCT GTGTTCAATA CGCTTACACC AGCCATCAAG 
GAGTCCTGGG TCAGCAGCCT GCAGATGGCC AAGCTGGCCC TTGAGGAGGA GAACCATATG 
GGATGGTTCT GTGTGGATGA CGATGGCAAC CTAGCCAAGA AGGAGACACA CCCTCTCCTG 
GTGGGACACA TGCCTGTGAT GGTGGCCAAG CAGCCAGAGT TCAAGATTGA ATGTGCAGCT 
TATAACCCTG AACCTTACCT GAGCAATGAA AGTCAACCAG CCTCACCCTC CACAGCCCGT 
GGCTTTCTGT GGATTGGAAG CTGTAGCAAC CAGATGGGTC AGGTCGCCAT TGTCTCCTTC 
CAAGGTTCCA ACCCCAAAGT CATCGAGTGC TTCAATGTGG AATCACGCAT CCTGTGTATG 
GTATACATTC CCGCAGAGGA GTCAAAGCCC CAAGAAACCA CCGAAACCAA GGATCCAGAA 
GCCACAGCCT CGAGAGCACC CCATGTGCCC ACCATCTGCC TGGGCACAGA AGAGGGAAGC 
ATTTCCATTT ACAAAAGCAG TCAGGGCTGC AAGAAGGTGA GGCTTCAGCA CTTCTATGCA 
CCGGACAAGT CCACGGTCAT GAGCTTGGCC TGCTCCCCAC AAGGCCTGTA TGCAGGCCTG 
GTCAATGGGT CCGTAGCCAG CTACACCAAG GCTCCAGCAC CGAGCTCTCC TGGCAGCCCG 
TGGGCAGAGT TCTGTCTCAT CTGCGTTTTC CTGCCATGCT TGCCCTCGGG AGCATATGCC 
ATCTCGGGTA CAGGCTGTGG TGCAGTGAAG GGCTATGGAT CCTGGAACTC GGAACCCCAG 
CAAGTGATCA AGTTGGGTGT CCTGCCAGTC AGAAGCCTTC TCCTGGTGGA GGGTGCACTG 
TGGGCAGCCT CGGGAGGGCA GGTGTTCATG GCCAGCGTGG AGACTCACAC TATAGAGAAT 
CAGCTGGAGG CCCACCAAGA TGAAGGGATG GTCATCTCCC ACATGGCTGT GGCTGGCGTT 
GGCATCTGGA TTGCCTTCAC CTCGGGGTCC ACCCTCCGCC TCTTCCACAC AGAGACCTTG 
AAGCACCTGC AGGATGTCAA CATTGATGCC CCTGTGCATA GCATGCTGCC AGGGCACCAG 
CGTCTATCTG TGACCAGCCT GCTCGTCTGC CACGGCTTGC TCATGGTCGG TACCAGCCTG 
GGTGTCGTGG TGGCCCTGCC AGTCCCTCGG CTTCAGGGTA TCCCCAAAGT GACAGGGCGA 
GGCATGGTCT CCTACCATGC GCATAACGGG CCCGTTAAGT TCATCGTCTC AGCCACGGCG 
TTTCAAAACA AGGACAGAGC CAGGGACAGC CCACGTTCCG GTTCTGAGCT TCAGGATGAA 
GACCCCAAGG ACTTGCTCTG CAGCGAGGAA GGTCCTTCCT GCCCGGGCCA GCCTGACACC 
TACACATCCG TTTGGTTGGG AGACTCCCTA GGATTGCCAA CTCAAAAGAA TGATCTGTCT 
TCGTCCTCGG GGTCACTGAA CCTGTCCCAT GGCTCCAGTT CCCTAGAGCA CAGATCTGTG 
GACAGCAGTC TGTGTGACCT GCTGAGGGAC CCCAGTGCCT CCCCAAGGAG CAGACCACAG 
GGCTCTAGGC GAGCAAGAGC CAGTTCAGCG CTGGTGGTAT GTGGGGGCCA GGGCCACCGG 
CGGGTGCACA GGAAGGCCAG GCAGCCTTCT CAGGAGGACC TGGTTTCTTC AGTGATGGTG 
TGGCAGATCC CCCTGCTGGG CATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508831.1
CDS	78..4304
Translation	MAPGPSGPLSPDAVQAWRKEARLSMEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQLSHDLTRFKAHCEEKMRGLVASTVGAMEIQQAKQRQERKMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPAPSSPGSPWAEFCLICVFLPCLPSGAYAISGTGCGAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_006508768.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508768.3

ORF Insert Sequence:

ATGGCGCCCG GTCCCTCGGG GCCCCTGTCC CCTGATGCTG TCCAAGCGTG GAGGAAGGAG 
GCAAGGCTGA GCATGGAGCA GGGAGAGGCC TCACCTCCTG TTCCTGCAGA ACATGAAGCG 
AAATGTGACA CCAGCAACAA CGAAGAGGAG GGAGAACTGT TTGACTTCGA CAGTGGAGAT 
GAAGTTCCCG AAGCGGATCG GCAAGTCCCG TCTGCCGACG ATAGGACGAG AGGTGCTGAA 
GCAGGAGGTG CAGACGAGAA CACGTGCCCT GCAGGAAATG GAACTGGAGC TGAACCTGCC 
CCCGAGGCAG AGCCTGCGAA ACTTGTGGTC CCCACAAAAG TGAACCCGTA TTCTGTCATC 
GACATCACGC CGCTGCAGGA AGACCAGCCG TCATCTCCAG ACGCGAATAC AGAGGAGGAA 
GGCGTGGGGC TGCGTGTGCC CAGTGGTTAC TCTGTGCCTG TGCCCTGTGG CTATGCTGTC 
CCCTCCAACC TGCCATTGCT GCTGCCTGCC TACTCCAGTC CTGTCATCAT CCGAGCCGAG 
TCTGTAGAGG AGGAAGAAGC AGCAGAAACT GTGGGAGATG GGCAGTGCAA TTCCCTAAGC 
TCCGAGGACC TTCCACACAG CAGTGAGCAA GGCAGCCAAG AAGGCAGTGC CCTGGCCCGA 
TGGGCAGCCG ACCCTGCCAA CACAGCATGG ATGGAAAATC CAGAGGAAGC AATTTACGAC 
GACGTTCCGA GGGAAAACTC GGACTCTGAG CCAGATGAAA TGATTTATGA TGATGTGGAG 
AACGGCGAAG AAGGTGGAAA CAGCTCCCCA GAGTACGGGT GGAGTTCCAG CGAATTCGAA 
AGCTATGAGG AGCCAAGCGA CTCGGAGGGC AAGAACGGAA TTCCCAGGTC CTTCCTGCGC 
AGCAGTCATA AAAAGCAACT CTCTCATGAC CTAACCCGCT TTAAGGCGCA CTGTGAGGAA 
AAGATGAGAG GCTTGGTGGC CAGTACGGTG GGAGCCATGG AGATTCAGCA GGCAAAGCAG 
AGGCAGGAGC GGAAGATGCA GAAGCTTATG AAGGCAGCCA AGGAGGGCAC CAAAGATGGG 
CTGGAGAAAA CCAAAGCTGC TGTGAAGAGA GGCGGCTCCT TCATCAGGAC CAGGTCCTTG 
GTCTCTCAAG ATCACAGGTG TTATTTTGAA GAAGAGCAGA ATTTGTTCAT TGACGTGGAC 
TGCAAGCACC CGGAGGCTGT CCTCACCCCA ATGCCCGAGG GCTTGTCTCA GCAGCAGGTT 
GTGAGAAGAT ACATTCTGGG CTCCATTGTT GAGAGCGAGA AGAACTATGT AGATGCTCTC 
CGGAGGATTT TGGAGCAATA TGAGAAGCCG CTGTCTGAGA TGGAGCCCCG GCTCCTGAGT 
GACAGGAAGC TCAGGATGGT TTTCTACCGC GTCAAGGAGA TCCTGCAGTG TCACTCCATG 
TTCCAGATTG CGCTGGCCAG CCGCGTGTCC GAGTGGGATG TCGTGGAAAC AATCGGCGAC 
GTTTTTGTGG CCTCGTTCTC CAAGTCCATG GTGCTGGATG CTTACAGCGA GTACGTGAAC 
AACTTCAGCA CGGCCGTGGC TGTCCTCAAG AAGACGTGTG CCACAAAGCC TGCTTTCCTT 
GAGTTCTTAA AGCTGAGCCA GGATTCCAGT CCAGACCGAG TCACACTCCA CAGCCTTATG 
ATGCGGCCCA TCCAGAGGTT CCCACAGTTC ATCCTCCTAC TGCAGGACAT GCTGAAGAAC 
ACCGCCAAAG GACATCCTGA CAGGCTGCCC CTTCAGATGG CGCTGACGGA GCTCGAGACC 
CTGGCAGAGA AGCTAAACGA GAGAAAAAGA GATGCCGACC AGCGATGCGA AATCAAACAA 
ATAGCAAAAG CCATAAACGA GAGATACCTG AACAAGCTCC TCAGCAGTGG AAACCGGTAC 
CTCGTCCGGT CCGATGACGT CATCGAGACG GTTTACAACG ACCGGGGAGA AATCGTCAAG 
ACCAAACAAC GCCGGATCTT CATGCTGAAT GACGTGCTCA TGTGTGCCAC CGCCAGCTCA 
CGGAACTCAC ATGAAAGCCA TGCCGTGATG AGCCAGAGGT ACCTGCTCAA GTGGAGTGTC 
CCTCTAGGTC ACGTGGATGT GATTCAGTAC AATGGAGGCT CTGGCGCCGG GGAGCACTGT 
CGGCACCACG CTGCACACTC ACCTGAGAGC CTGGCCGTGG TGGCCAACGC GAAGCCACAC 
AAAGTTTACA TGGGGCCAGG GCAGCTGTAC CAGGATCTGC AGAACCTACT GCACGACCTG 
AACGTTGTTG GCCAGATCTC GCAGCTGATA GGAAACCTCC GAGGGAGCTA TCAGAACTTA 
AACCAGTCTG TAGCCCATGA TTGGACGTCG GGCTTACAGC GGCTGATACT GAGGAAAGAA 
GATGCGATCA GAGCTGCCGA CCGCTGCCGG ATCCAGTTAC AGCTCCCGGG AAAGCAGGAC 
AAGTCCGGGC GGCCCACGTT CTTCACTGCT GTGTTCAATA CGCTTACACC AGCCATCAAG 
GAGTCCTGGG TCAGCAGCCT GCAGATGGCC AAGCTGGCCC TTGAGGAGGA GAACCATATG 
GGATGGTTCT GTGTGGATGA CGATGGCAAC CTAGCCAAGA AGGAGACACA CCCTCTCCTG 
GTGGGACACA TGCCTGTGAT GGTGGCCAAG CAGCCAGAGT TCAAGATTGA ATGTGCAGCT 
TATAACCCTG AACCTTACCT GAGCAATGAA AGTCAACCAG CCTCACCCTC CACAGCCCGT 
GGCTTTCTGT GGATTGGAAG CTGTAGCAAC CAGATGGGTC AGGTCGCCAT TGTCTCCTTC 
CAAGGTTCCA ACCCCAAAGT CATCGAGTGC TTCAATGTGG AATCACGCAT CCTGTGTATG 
GTATACATTC CCGCAGAGGA GTCAAAGCCC CAAGAAACCA CCGAAACCAA GGATCCAGAA 
GCCACAGCCT CGAGAGCACC CCATGTGCCC ACCATCTGCC TGGGCACAGA AGAGGGAAGC 
ATTTCCATTT ACAAAAGCAG TCAGGGCTGC AAGAAGGTGA GGCTTCAGCA CTTCTATGCA 
CCGGACAAGT CCACGGTCAT GAGCTTGGCC TGCTCCCCAC AAGGCCTGTA TGCAGGCCTG 
GTCAATGGGT CCGTAGCCAG CTACACCAAG GCTCCAGCAC CGAGCTCTCC TGGCAGCCCG 
TGGGCAGAGT TCTGTCTCAT CTGCGTTTTC CTGCCATGCT TGCCCTCGGG AGCATATGCC 
ATCTCGGGTA CAGGCTGTGG TGCAGTGAAG GGCTATGGAT CCTGGAACTC GGAACCCCAG 
CAAGTGATCA AGTTGGGTGT CCTGCCAGTC AGAAGCCTTC TCCTGGTGGA GGGTGCACTG 
TGGGCAGCCT CGGGAGGGCA GGTGTTCATG GCCAGCGTGG AGACTCACAC TATAGAGAAT 
CAGCTGGAGG CCCACCAAGA TGAAGGGATG GTCATCTCCC ACATGGCTGT GGCTGGCGTT 
GGCATCTGGA TTGCCTTCAC CTCGGGGTCC ACCCTCCGCC TCTTCCACAC AGAGACCTTG 
AAGCACCTGC AGGATGTCAA CATTGATGCC CCTGTGCATA GCATGCTGCC AGGGCACCAG 
CGTCTATCTG TGACCAGCCT GCTCGTCTGC CACGGCTTGC TCATGGTCGG TACCAGCCTG 
GGTGTCGTGG TGGCCCTGCC AGTCCCTCGG CTTCAGGGTA TCCCCAAAGT GACAGGGCGA 
GGCATGGTCT CCTACCATGC GCATAACGGG CCCGTTAAGT TCATCGTCTC AGCCACGGCG 
TTTCAAAACA AGGACAGAGC CAGGGACAGC CCACGTTCCG GTTCTGAGCT TCAGGATGAA 
GACCCCAAGG ACTTGCTCTG CAGCGAGGAA GGTCCTTCCT GCCCGGGCCA GCCTGACACC 
TACACATCCG TTTGGTTGGG AGACTCCCTA GGATTGCCAA CTCAAAAGAA TGATCTGTCT 
TCGTCCTCGG GGTCACTGAA CCTGTCCCAT GGCTCCAGTT CCCTAGAGCA CAGATCTGTG 
GACAGCAGTC TGTGTGACCT GCTGAGGGAC CCCAGTGCCT CCCCAAGGAG CAGACCACAG 
GGCTCTAGGC GAGCAAGAGC CAGTTCAGCG CTGGTGGTAT GTGGGGGCCA GGGCCACCGG 
CGGGTGCACA GGAAGGCCAG GCAGCCTTCT CAGGAGGACC TGGTTTCTTC AGTGATGGTG 
TGGCAGATCC CCCTGCTGGG CATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_006508771.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508834.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X8, mRNA.

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CloneID	OMu40384
Clone ID Related Accession (Same CDS sequence)	XM_006508771.3 , XM_006508770.3 , XM_006508771.4 , XM_030243425.1 , XM_017312673.1
Accession Version	XM_006508771.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508771.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAACTCT CTCATGACCT AACCCGCTTT AAGGCGCACT GTGAGGAAAA GATGAGAGGC 
TTGGTGGCCA GTACGGTGGG AGCCATGGAG ATTCAGCAGG CAAAGCAGAG GCAGGAGCGG 
AAGATGCAGA AGCTTATGAA GGCAGCCAAG GAGGGCACCA AAGATGGGCT GGAGAAAACC 
AAAGCTGCTG TGAAGAGAGG CGGCTCCTTC ATCAGGACCA GGTCCTTGGT CTCTCAAGAT 
CACAGGTGTT ATTTTGAAGA AGAGCAGAAT TTGTTCATTG ACGTGGACTG CAAGCACCCG 
GAGGCTGTCC TCACCCCAAT GCCCGAGGGC TTGTCTCAGC AGCAGGTTGT GAGAAGATAC 
ATTCTGGGCT CCATTGTTGA GAGCGAGAAG AACTATGTAG ATGCTCTCCG GAGGATTTTG 
GAGCAATATG AGAAGCCGCT GTCTGAGATG GAGCCCCGGC TCCTGAGTGA CAGGAAGCTC 
AGGATGGTTT TCTACCGCGT CAAGGAGATC CTGCAGTGTC ACTCCATGTT CCAGATTGCG 
CTGGCCAGCC GCGTGTCCGA GTGGGATGTC GTGGAAACAA TCGGCGACGT TTTTGTGGCC 
TCGTTCTCCA AGTCCATGGT GCTGGATGCT TACAGCGAGT ACGTGAACAA CTTCAGCACG 
GCCGTGGCTG TCCTCAAGAA GACGTGTGCC ACAAAGCCTG CTTTCCTTGA GTTCTTAAAG 
CTGAGCCAGG ATTCCAGTCC AGACCGAGTC ACACTCCACA GCCTTATGAT GCGGCCCATC 
CAGAGGTTCC CACAGTTCAT CCTCCTACTG CAGGACATGC TGAAGAACAC CGCCAAAGGA 
CATCCTGACA GGCTGCCCCT TCAGATGGCG CTGACGGAGC TCGAGACCCT GGCAGAGAAG 
CTAAACGAGA GAAAAAGAGA TGCCGACCAG CGATGCGAAA TCAAACAAAT AGCAAAAGCC 
ATAAACGAGA GATACCTGAA CAAGCTCCTC AGCAGTGGAA ACCGGTACCT CGTCCGGTCC 
GATGACGTCA TCGAGACGGT TTACAACGAC CGGGGAGAAA TCGTCAAGAC CAAACAACGC 
CGGATCTTCA TGCTGAATGA CGTGCTCATG TGTGCCACCG CCAGCTCACG GAACTCACAT 
GAAAGCCATG CCGTGATGAG CCAGAGGTAC CTGCTCAAGT GGAGTGTCCC TCTAGGTCAC 
GTGGATGTGA TTCAGTACAA TGGAGGCTCT GGCGCCGGGG AGCACTGTCG GCACCACGCT 
GCACACTCAC CTGAGAGCCT GGCCGTGGTG GCCAACGCGA AGCCACACAA AGTTTACATG 
GGGCCAGGGC AGCTGTACCA GGATCTGCAG AACCTACTGC ACGACCTGAA CGTTGTTGGC 
CAGATCTCGC AGCTGATAGG AAACCTCCGA GGGAGCTATC AGAACTTAAA CCAGTCTGTA 
GCCCATGATT GGACGTCGGG CTTACAGCGG CTGATACTGA GGAAAGAAGA TGCGATCAGA 
GCTGCCGACC GCTGCCGGAT CCAGTTACAG CTCCCGGGAA AGCAGGACAA GTCCGGGCGG 
CCCACGTTCT TCACTGCTGT GTTCAATACG CTTACACCAG CCATCAAGGA GTCCTGGGTC 
AGCAGCCTGC AGATGGCCAA GCTGGCCCTT GAGGAGGAGA ACCATATGGG ATGGTTCTGT 
GTGGATGACG ATGGCAACCT AGCCAAGAAG GAGACACACC CTCTCCTGGT GGGACACATG 
CCTGTGATGG TGGCCAAGCA GCCAGAGTTC AAGATTGAAT GTGCAGCTTA TAACCCTGAA 
CCTTACCTGA GCAATGAAAG TCAACCAGCC TCACCCTCCA CAGCCCGTGG CTTTCTGTGG 
ATTGGAAGCT GTAGCAACCA GATGGGTCAG GTCGCCATTG TCTCCTTCCA AGGTTCCAAC 
CCCAAAGTCA TCGAGTGCTT CAATGTGGAA TCACGCATCC TGTGTATGGT ATACATTCCC 
GCAGAGGAGT CAAAGCCCCA AGAAACCACC GAAACCAAGG ATCCAGAAGC CACAGCCTCG 
AGAGCACCCC ATGTGCCCAC CATCTGCCTG GGCACAGAAG AGGGAAGCAT TTCCATTTAC 
AAAAGCAGTC AGGGCTGCAA GAAGGTGAGG CTTCAGCACT TCTATGCACC GGACAAGTCC 
ACGGTCATGA GCTTGGCCTG CTCCCCACAA GGCCTGTATG CAGGCCTGGT CAATGGGTCC 
GTAGCCAGCT ACACCAAGGC TCCAGCACCG AGCTCTCCTG GCAGCCCGTG GGCAGAGTTC 
TGTCTCATCT GCGTTTTCCT GCCATGCTTG CCCTCGGGAG CATATGCCAT CTCGGGTACA 
GGCTGTGGTG CAGTGAAGGG CTATGGATCC TGGAACTCGG AACCCCAGCA AGTGATCAAG 
TTGGGTGTCC TGCCAGTCAG AAGCCTTCTC CTGGTGGAGG GTGCACTGTG GGCAGCCTCG 
GGAGGGCAGG TGTTCATGGC CAGCGTGGAG ACTCACACTA TAGAGAATCA GCTGGAGGCC 
CACCAAGATG AAGGGATGGT CATCTCCCAC ATGGCTGTGG CTGGCGTTGG CATCTGGATT 
GCCTTCACCT CGGGGTCCAC CCTCCGCCTC TTCCACACAG AGACCTTGAA GCACCTGCAG 
GATGTCAACA TTGATGCCCC TGTGCATAGC ATGCTGCCAG GGCACCAGCG TCTATCTGTG 
ACCAGCCTGC TCGTCTGCCA CGGCTTGCTC ATGGTCGGTA CCAGCCTGGG TGTCGTGGTG 
GCCCTGCCAG TCCCTCGGCT TCAGGGTATC CCCAAAGTGA CAGGGCGAGG CATGGTCTCC 
TACCATGCGC ATAACGGGCC CGTTAAGTTC ATCGTCTCAG CCACGGCGTT TCAAAACAAG 
GACAGAGCCA GGGACAGCCC ACGTTCCGGT TCTGAGCTTC AGGATGAAGA CCCCAAGGAC 
TTGCTCTGCA GCGAGGAAGG TCCTTCCTGC CCGGGCCAGC CTGACACCTA CACATCCGTT 
TGGTTGGGAG ACTCCCTAGG ATTGCCAACT CAAAAGAATG ATCTGTCTTC GTCCTCGGGG 
TCACTGAACC TGTCCCATGG CTCCAGTTCC CTAGAGCACA GATCTGTGGA CAGCAGTCTG 
TGTGACCTGC TGAGGGACCC CAGTGCCTCC CCAAGGAGCA GACCACAGGG CTCTAGGCGA 
GCAAGAGCCA GTTCAGCGCT GGTGGTATGT GGGGGCCAGG GCCACCGGCG GGTGCACAGG 
AAGGCCAGGC AGCCTTCTCA GGAGGACCTG GTTTCTTCAG TGATGGTGTG GCAGATCCCC 
CTGCTGGGCA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508834.1
CDS	650..4804
Translation	MEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQLSHDLTRFKAHCEEKMRGLVASTVGAMEIQQAKQRQERKMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPAPSSPGSPWAEFCLICVFLPCLPSGAYAISGTGCGAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_006508771.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508771.3

ORF Insert Sequence:

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAACTCT CTCATGACCT AACCCGCTTT AAGGCGCACT GTGAGGAAAA GATGAGAGGC 
TTGGTGGCCA GTACGGTGGG AGCCATGGAG ATTCAGCAGG CAAAGCAGAG GCAGGAGCGG 
AAGATGCAGA AGCTTATGAA GGCAGCCAAG GAGGGCACCA AAGATGGGCT GGAGAAAACC 
AAAGCTGCTG TGAAGAGAGG CGGCTCCTTC ATCAGGACCA GGTCCTTGGT CTCTCAAGAT 
CACAGGTGTT ATTTTGAAGA AGAGCAGAAT TTGTTCATTG ACGTGGACTG CAAGCACCCG 
GAGGCTGTCC TCACCCCAAT GCCCGAGGGC TTGTCTCAGC AGCAGGTTGT GAGAAGATAC 
ATTCTGGGCT CCATTGTTGA GAGCGAGAAG AACTATGTAG ATGCTCTCCG GAGGATTTTG 
GAGCAATATG AGAAGCCGCT GTCTGAGATG GAGCCCCGGC TCCTGAGTGA CAGGAAGCTC 
AGGATGGTTT TCTACCGCGT CAAGGAGATC CTGCAGTGTC ACTCCATGTT CCAGATTGCG 
CTGGCCAGCC GCGTGTCCGA GTGGGATGTC GTGGAAACAA TCGGCGACGT TTTTGTGGCC 
TCGTTCTCCA AGTCCATGGT GCTGGATGCT TACAGCGAGT ACGTGAACAA CTTCAGCACG 
GCCGTGGCTG TCCTCAAGAA GACGTGTGCC ACAAAGCCTG CTTTCCTTGA GTTCTTAAAG 
CTGAGCCAGG ATTCCAGTCC AGACCGAGTC ACACTCCACA GCCTTATGAT GCGGCCCATC 
CAGAGGTTCC CACAGTTCAT CCTCCTACTG CAGGACATGC TGAAGAACAC CGCCAAAGGA 
CATCCTGACA GGCTGCCCCT TCAGATGGCG CTGACGGAGC TCGAGACCCT GGCAGAGAAG 
CTAAACGAGA GAAAAAGAGA TGCCGACCAG CGATGCGAAA TCAAACAAAT AGCAAAAGCC 
ATAAACGAGA GATACCTGAA CAAGCTCCTC AGCAGTGGAA ACCGGTACCT CGTCCGGTCC 
GATGACGTCA TCGAGACGGT TTACAACGAC CGGGGAGAAA TCGTCAAGAC CAAACAACGC 
CGGATCTTCA TGCTGAATGA CGTGCTCATG TGTGCCACCG CCAGCTCACG GAACTCACAT 
GAAAGCCATG CCGTGATGAG CCAGAGGTAC CTGCTCAAGT GGAGTGTCCC TCTAGGTCAC 
GTGGATGTGA TTCAGTACAA TGGAGGCTCT GGCGCCGGGG AGCACTGTCG GCACCACGCT 
GCACACTCAC CTGAGAGCCT GGCCGTGGTG GCCAACGCGA AGCCACACAA AGTTTACATG 
GGGCCAGGGC AGCTGTACCA GGATCTGCAG AACCTACTGC ACGACCTGAA CGTTGTTGGC 
CAGATCTCGC AGCTGATAGG AAACCTCCGA GGGAGCTATC AGAACTTAAA CCAGTCTGTA 
GCCCATGATT GGACGTCGGG CTTACAGCGG CTGATACTGA GGAAAGAAGA TGCGATCAGA 
GCTGCCGACC GCTGCCGGAT CCAGTTACAG CTCCCGGGAA AGCAGGACAA GTCCGGGCGG 
CCCACGTTCT TCACTGCTGT GTTCAATACG CTTACACCAG CCATCAAGGA GTCCTGGGTC 
AGCAGCCTGC AGATGGCCAA GCTGGCCCTT GAGGAGGAGA ACCATATGGG ATGGTTCTGT 
GTGGATGACG ATGGCAACCT AGCCAAGAAG GAGACACACC CTCTCCTGGT GGGACACATG 
CCTGTGATGG TGGCCAAGCA GCCAGAGTTC AAGATTGAAT GTGCAGCTTA TAACCCTGAA 
CCTTACCTGA GCAATGAAAG TCAACCAGCC TCACCCTCCA CAGCCCGTGG CTTTCTGTGG 
ATTGGAAGCT GTAGCAACCA GATGGGTCAG GTCGCCATTG TCTCCTTCCA AGGTTCCAAC 
CCCAAAGTCA TCGAGTGCTT CAATGTGGAA TCACGCATCC TGTGTATGGT ATACATTCCC 
GCAGAGGAGT CAAAGCCCCA AGAAACCACC GAAACCAAGG ATCCAGAAGC CACAGCCTCG 
AGAGCACCCC ATGTGCCCAC CATCTGCCTG GGCACAGAAG AGGGAAGCAT TTCCATTTAC 
AAAAGCAGTC AGGGCTGCAA GAAGGTGAGG CTTCAGCACT TCTATGCACC GGACAAGTCC 
ACGGTCATGA GCTTGGCCTG CTCCCCACAA GGCCTGTATG CAGGCCTGGT CAATGGGTCC 
GTAGCCAGCT ACACCAAGGC TCCAGCACCG AGCTCTCCTG GCAGCCCGTG GGCAGAGTTC 
TGTCTCATCT GCGTTTTCCT GCCATGCTTG CCCTCGGGAG CATATGCCAT CTCGGGTACA 
GGCTGTGGTG CAGTGAAGGG CTATGGATCC TGGAACTCGG AACCCCAGCA AGTGATCAAG 
TTGGGTGTCC TGCCAGTCAG AAGCCTTCTC CTGGTGGAGG GTGCACTGTG GGCAGCCTCG 
GGAGGGCAGG TGTTCATGGC CAGCGTGGAG ACTCACACTA TAGAGAATCA GCTGGAGGCC 
CACCAAGATG AAGGGATGGT CATCTCCCAC ATGGCTGTGG CTGGCGTTGG CATCTGGATT 
GCCTTCACCT CGGGGTCCAC CCTCCGCCTC TTCCACACAG AGACCTTGAA GCACCTGCAG 
GATGTCAACA TTGATGCCCC TGTGCATAGC ATGCTGCCAG GGCACCAGCG TCTATCTGTG 
ACCAGCCTGC TCGTCTGCCA CGGCTTGCTC ATGGTCGGTA CCAGCCTGGG TGTCGTGGTG 
GCCCTGCCAG TCCCTCGGCT TCAGGGTATC CCCAAAGTGA CAGGGCGAGG CATGGTCTCC 
TACCATGCGC ATAACGGGCC CGTTAAGTTC ATCGTCTCAG CCACGGCGTT TCAAAACAAG 
GACAGAGCCA GGGACAGCCC ACGTTCCGGT TCTGAGCTTC AGGATGAAGA CCCCAAGGAC 
TTGCTCTGCA GCGAGGAAGG TCCTTCCTGC CCGGGCCAGC CTGACACCTA CACATCCGTT 
TGGTTGGGAG ACTCCCTAGG ATTGCCAACT CAAAAGAATG ATCTGTCTTC GTCCTCGGGG 
TCACTGAACC TGTCCCATGG CTCCAGTTCC CTAGAGCACA GATCTGTGGA CAGCAGTCTG 
TGTGACCTGC TGAGGGACCC CAGTGCCTCC CCAAGGAGCA GACCACAGGG CTCTAGGCGA 
GCAAGAGCCA GTTCAGCGCT GGTGGTATGT GGGGGCCAGG GCCACCGGCG GGTGCACAGG 
AAGGCCAGGC AGCCTTCTCA GGAGGACCTG GTTTCTTCAG TGATGGTGTG GCAGATCCCC 
CTGCTGGGCA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_006508770.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508833.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X7, mRNA.

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CloneID	OMu40384
Clone ID Related Accession (Same CDS sequence)	XM_006508771.3 , XM_006508770.3 , XM_006508771.4 , XM_030243425.1 , XM_017312673.1
Accession Version	XM_006508770.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508770.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAACTCT CTCATGACCT AACCCGCTTT AAGGCGCACT GTGAGGAAAA GATGAGAGGC 
TTGGTGGCCA GTACGGTGGG AGCCATGGAG ATTCAGCAGG CAAAGCAGAG GCAGGAGCGG 
AAGATGCAGA AGCTTATGAA GGCAGCCAAG GAGGGCACCA AAGATGGGCT GGAGAAAACC 
AAAGCTGCTG TGAAGAGAGG CGGCTCCTTC ATCAGGACCA GGTCCTTGGT CTCTCAAGAT 
CACAGGTGTT ATTTTGAAGA AGAGCAGAAT TTGTTCATTG ACGTGGACTG CAAGCACCCG 
GAGGCTGTCC TCACCCCAAT GCCCGAGGGC TTGTCTCAGC AGCAGGTTGT GAGAAGATAC 
ATTCTGGGCT CCATTGTTGA GAGCGAGAAG AACTATGTAG ATGCTCTCCG GAGGATTTTG 
GAGCAATATG AGAAGCCGCT GTCTGAGATG GAGCCCCGGC TCCTGAGTGA CAGGAAGCTC 
AGGATGGTTT TCTACCGCGT CAAGGAGATC CTGCAGTGTC ACTCCATGTT CCAGATTGCG 
CTGGCCAGCC GCGTGTCCGA GTGGGATGTC GTGGAAACAA TCGGCGACGT TTTTGTGGCC 
TCGTTCTCCA AGTCCATGGT GCTGGATGCT TACAGCGAGT ACGTGAACAA CTTCAGCACG 
GCCGTGGCTG TCCTCAAGAA GACGTGTGCC ACAAAGCCTG CTTTCCTTGA GTTCTTAAAG 
CTGAGCCAGG ATTCCAGTCC AGACCGAGTC ACACTCCACA GCCTTATGAT GCGGCCCATC 
CAGAGGTTCC CACAGTTCAT CCTCCTACTG CAGGACATGC TGAAGAACAC CGCCAAAGGA 
CATCCTGACA GGCTGCCCCT TCAGATGGCG CTGACGGAGC TCGAGACCCT GGCAGAGAAG 
CTAAACGAGA GAAAAAGAGA TGCCGACCAG CGATGCGAAA TCAAACAAAT AGCAAAAGCC 
ATAAACGAGA GATACCTGAA CAAGCTCCTC AGCAGTGGAA ACCGGTACCT CGTCCGGTCC 
GATGACGTCA TCGAGACGGT TTACAACGAC CGGGGAGAAA TCGTCAAGAC CAAACAACGC 
CGGATCTTCA TGCTGAATGA CGTGCTCATG TGTGCCACCG CCAGCTCACG GAACTCACAT 
GAAAGCCATG CCGTGATGAG CCAGAGGTAC CTGCTCAAGT GGAGTGTCCC TCTAGGTCAC 
GTGGATGTGA TTCAGTACAA TGGAGGCTCT GGCGCCGGGG AGCACTGTCG GCACCACGCT 
GCACACTCAC CTGAGAGCCT GGCCGTGGTG GCCAACGCGA AGCCACACAA AGTTTACATG 
GGGCCAGGGC AGCTGTACCA GGATCTGCAG AACCTACTGC ACGACCTGAA CGTTGTTGGC 
CAGATCTCGC AGCTGATAGG AAACCTCCGA GGGAGCTATC AGAACTTAAA CCAGTCTGTA 
GCCCATGATT GGACGTCGGG CTTACAGCGG CTGATACTGA GGAAAGAAGA TGCGATCAGA 
GCTGCCGACC GCTGCCGGAT CCAGTTACAG CTCCCGGGAA AGCAGGACAA GTCCGGGCGG 
CCCACGTTCT TCACTGCTGT GTTCAATACG CTTACACCAG CCATCAAGGA GTCCTGGGTC 
AGCAGCCTGC AGATGGCCAA GCTGGCCCTT GAGGAGGAGA ACCATATGGG ATGGTTCTGT 
GTGGATGACG ATGGCAACCT AGCCAAGAAG GAGACACACC CTCTCCTGGT GGGACACATG 
CCTGTGATGG TGGCCAAGCA GCCAGAGTTC AAGATTGAAT GTGCAGCTTA TAACCCTGAA 
CCTTACCTGA GCAATGAAAG TCAACCAGCC TCACCCTCCA CAGCCCGTGG CTTTCTGTGG 
ATTGGAAGCT GTAGCAACCA GATGGGTCAG GTCGCCATTG TCTCCTTCCA AGGTTCCAAC 
CCCAAAGTCA TCGAGTGCTT CAATGTGGAA TCACGCATCC TGTGTATGGT ATACATTCCC 
GCAGAGGAGT CAAAGCCCCA AGAAACCACC GAAACCAAGG ATCCAGAAGC CACAGCCTCG 
AGAGCACCCC ATGTGCCCAC CATCTGCCTG GGCACAGAAG AGGGAAGCAT TTCCATTTAC 
AAAAGCAGTC AGGGCTGCAA GAAGGTGAGG CTTCAGCACT TCTATGCACC GGACAAGTCC 
ACGGTCATGA GCTTGGCCTG CTCCCCACAA GGCCTGTATG CAGGCCTGGT CAATGGGTCC 
GTAGCCAGCT ACACCAAGGC TCCAGCACCG AGCTCTCCTG GCAGCCCGTG GGCAGAGTTC 
TGTCTCATCT GCGTTTTCCT GCCATGCTTG CCCTCGGGAG CATATGCCAT CTCGGGTACA 
GGCTGTGGTG CAGTGAAGGG CTATGGATCC TGGAACTCGG AACCCCAGCA AGTGATCAAG 
TTGGGTGTCC TGCCAGTCAG AAGCCTTCTC CTGGTGGAGG GTGCACTGTG GGCAGCCTCG 
GGAGGGCAGG TGTTCATGGC CAGCGTGGAG ACTCACACTA TAGAGAATCA GCTGGAGGCC 
CACCAAGATG AAGGGATGGT CATCTCCCAC ATGGCTGTGG CTGGCGTTGG CATCTGGATT 
GCCTTCACCT CGGGGTCCAC CCTCCGCCTC TTCCACACAG AGACCTTGAA GCACCTGCAG 
GATGTCAACA TTGATGCCCC TGTGCATAGC ATGCTGCCAG GGCACCAGCG TCTATCTGTG 
ACCAGCCTGC TCGTCTGCCA CGGCTTGCTC ATGGTCGGTA CCAGCCTGGG TGTCGTGGTG 
GCCCTGCCAG TCCCTCGGCT TCAGGGTATC CCCAAAGTGA CAGGGCGAGG CATGGTCTCC 
TACCATGCGC ATAACGGGCC CGTTAAGTTC ATCGTCTCAG CCACGGCGTT TCAAAACAAG 
GACAGAGCCA GGGACAGCCC ACGTTCCGGT TCTGAGCTTC AGGATGAAGA CCCCAAGGAC 
TTGCTCTGCA GCGAGGAAGG TCCTTCCTGC CCGGGCCAGC CTGACACCTA CACATCCGTT 
TGGTTGGGAG ACTCCCTAGG ATTGCCAACT CAAAAGAATG ATCTGTCTTC GTCCTCGGGG 
TCACTGAACC TGTCCCATGG CTCCAGTTCC CTAGAGCACA GATCTGTGGA CAGCAGTCTG 
TGTGACCTGC TGAGGGACCC CAGTGCCTCC CCAAGGAGCA GACCACAGGG CTCTAGGCGA 
GCAAGAGCCA GTTCAGCGCT GGTGGTATGT GGGGGCCAGG GCCACCGGCG GGTGCACAGG 
AAGGCCAGGC AGCCTTCTCA GGAGGACCTG GTTTCTTCAG TGATGGTGTG GCAGATCCCC 
CTGCTGGGCA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508833.1
CDS	236..4390
Translation	MEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQLSHDLTRFKAHCEEKMRGLVASTVGAMEIQQAKQRQERKMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPAPSSPGSPWAEFCLICVFLPCLPSGAYAISGTGCGAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_006508770.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508770.3

ORF Insert Sequence:

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAACTCT CTCATGACCT AACCCGCTTT AAGGCGCACT GTGAGGAAAA GATGAGAGGC 
TTGGTGGCCA GTACGGTGGG AGCCATGGAG ATTCAGCAGG CAAAGCAGAG GCAGGAGCGG 
AAGATGCAGA AGCTTATGAA GGCAGCCAAG GAGGGCACCA AAGATGGGCT GGAGAAAACC 
AAAGCTGCTG TGAAGAGAGG CGGCTCCTTC ATCAGGACCA GGTCCTTGGT CTCTCAAGAT 
CACAGGTGTT ATTTTGAAGA AGAGCAGAAT TTGTTCATTG ACGTGGACTG CAAGCACCCG 
GAGGCTGTCC TCACCCCAAT GCCCGAGGGC TTGTCTCAGC AGCAGGTTGT GAGAAGATAC 
ATTCTGGGCT CCATTGTTGA GAGCGAGAAG AACTATGTAG ATGCTCTCCG GAGGATTTTG 
GAGCAATATG AGAAGCCGCT GTCTGAGATG GAGCCCCGGC TCCTGAGTGA CAGGAAGCTC 
AGGATGGTTT TCTACCGCGT CAAGGAGATC CTGCAGTGTC ACTCCATGTT CCAGATTGCG 
CTGGCCAGCC GCGTGTCCGA GTGGGATGTC GTGGAAACAA TCGGCGACGT TTTTGTGGCC 
TCGTTCTCCA AGTCCATGGT GCTGGATGCT TACAGCGAGT ACGTGAACAA CTTCAGCACG 
GCCGTGGCTG TCCTCAAGAA GACGTGTGCC ACAAAGCCTG CTTTCCTTGA GTTCTTAAAG 
CTGAGCCAGG ATTCCAGTCC AGACCGAGTC ACACTCCACA GCCTTATGAT GCGGCCCATC 
CAGAGGTTCC CACAGTTCAT CCTCCTACTG CAGGACATGC TGAAGAACAC CGCCAAAGGA 
CATCCTGACA GGCTGCCCCT TCAGATGGCG CTGACGGAGC TCGAGACCCT GGCAGAGAAG 
CTAAACGAGA GAAAAAGAGA TGCCGACCAG CGATGCGAAA TCAAACAAAT AGCAAAAGCC 
ATAAACGAGA GATACCTGAA CAAGCTCCTC AGCAGTGGAA ACCGGTACCT CGTCCGGTCC 
GATGACGTCA TCGAGACGGT TTACAACGAC CGGGGAGAAA TCGTCAAGAC CAAACAACGC 
CGGATCTTCA TGCTGAATGA CGTGCTCATG TGTGCCACCG CCAGCTCACG GAACTCACAT 
GAAAGCCATG CCGTGATGAG CCAGAGGTAC CTGCTCAAGT GGAGTGTCCC TCTAGGTCAC 
GTGGATGTGA TTCAGTACAA TGGAGGCTCT GGCGCCGGGG AGCACTGTCG GCACCACGCT 
GCACACTCAC CTGAGAGCCT GGCCGTGGTG GCCAACGCGA AGCCACACAA AGTTTACATG 
GGGCCAGGGC AGCTGTACCA GGATCTGCAG AACCTACTGC ACGACCTGAA CGTTGTTGGC 
CAGATCTCGC AGCTGATAGG AAACCTCCGA GGGAGCTATC AGAACTTAAA CCAGTCTGTA 
GCCCATGATT GGACGTCGGG CTTACAGCGG CTGATACTGA GGAAAGAAGA TGCGATCAGA 
GCTGCCGACC GCTGCCGGAT CCAGTTACAG CTCCCGGGAA AGCAGGACAA GTCCGGGCGG 
CCCACGTTCT TCACTGCTGT GTTCAATACG CTTACACCAG CCATCAAGGA GTCCTGGGTC 
AGCAGCCTGC AGATGGCCAA GCTGGCCCTT GAGGAGGAGA ACCATATGGG ATGGTTCTGT 
GTGGATGACG ATGGCAACCT AGCCAAGAAG GAGACACACC CTCTCCTGGT GGGACACATG 
CCTGTGATGG TGGCCAAGCA GCCAGAGTTC AAGATTGAAT GTGCAGCTTA TAACCCTGAA 
CCTTACCTGA GCAATGAAAG TCAACCAGCC TCACCCTCCA CAGCCCGTGG CTTTCTGTGG 
ATTGGAAGCT GTAGCAACCA GATGGGTCAG GTCGCCATTG TCTCCTTCCA AGGTTCCAAC 
CCCAAAGTCA TCGAGTGCTT CAATGTGGAA TCACGCATCC TGTGTATGGT ATACATTCCC 
GCAGAGGAGT CAAAGCCCCA AGAAACCACC GAAACCAAGG ATCCAGAAGC CACAGCCTCG 
AGAGCACCCC ATGTGCCCAC CATCTGCCTG GGCACAGAAG AGGGAAGCAT TTCCATTTAC 
AAAAGCAGTC AGGGCTGCAA GAAGGTGAGG CTTCAGCACT TCTATGCACC GGACAAGTCC 
ACGGTCATGA GCTTGGCCTG CTCCCCACAA GGCCTGTATG CAGGCCTGGT CAATGGGTCC 
GTAGCCAGCT ACACCAAGGC TCCAGCACCG AGCTCTCCTG GCAGCCCGTG GGCAGAGTTC 
TGTCTCATCT GCGTTTTCCT GCCATGCTTG CCCTCGGGAG CATATGCCAT CTCGGGTACA 
GGCTGTGGTG CAGTGAAGGG CTATGGATCC TGGAACTCGG AACCCCAGCA AGTGATCAAG 
TTGGGTGTCC TGCCAGTCAG AAGCCTTCTC CTGGTGGAGG GTGCACTGTG GGCAGCCTCG 
GGAGGGCAGG TGTTCATGGC CAGCGTGGAG ACTCACACTA TAGAGAATCA GCTGGAGGCC 
CACCAAGATG AAGGGATGGT CATCTCCCAC ATGGCTGTGG CTGGCGTTGG CATCTGGATT 
GCCTTCACCT CGGGGTCCAC CCTCCGCCTC TTCCACACAG AGACCTTGAA GCACCTGCAG 
GATGTCAACA TTGATGCCCC TGTGCATAGC ATGCTGCCAG GGCACCAGCG TCTATCTGTG 
ACCAGCCTGC TCGTCTGCCA CGGCTTGCTC ATGGTCGGTA CCAGCCTGGG TGTCGTGGTG 
GCCCTGCCAG TCCCTCGGCT TCAGGGTATC CCCAAAGTGA CAGGGCGAGG CATGGTCTCC 
TACCATGCGC ATAACGGGCC CGTTAAGTTC ATCGTCTCAG CCACGGCGTT TCAAAACAAG 
GACAGAGCCA GGGACAGCCC ACGTTCCGGT TCTGAGCTTC AGGATGAAGA CCCCAAGGAC 
TTGCTCTGCA GCGAGGAAGG TCCTTCCTGC CCGGGCCAGC CTGACACCTA CACATCCGTT 
TGGTTGGGAG ACTCCCTAGG ATTGCCAACT CAAAAGAATG ATCTGTCTTC GTCCTCGGGG 
TCACTGAACC TGTCCCATGG CTCCAGTTCC CTAGAGCACA GATCTGTGGA CAGCAGTCTG 
TGTGACCTGC TGAGGGACCC CAGTGCCTCC CCAAGGAGCA GACCACAGGG CTCTAGGCGA 
GCAAGAGCCA GTTCAGCGCT GGTGGTATGT GGGGGCCAGG GCCACCGGCG GGTGCACAGG 
AAGGCCAGGC AGCCTTCTCA GGAGGACCTG GTTTCTTCAG TGATGGTGTG GCAGATCCCC 
CTGCTGGGCA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_017312673.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168162.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu40384
Clone ID Related Accession (Same CDS sequence)	XM_006508771.3 , XM_006508770.3 , XM_006508771.4 , XM_030243425.1 , XM_017312673.1
Accession Version	XM_017312673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAACTCT CTCATGACCT AACCCGCTTT AAGGCGCACT GTGAGGAAAA GATGAGAGGC 
TTGGTGGCCA GTACGGTGGG AGCCATGGAG ATTCAGCAGG CAAAGCAGAG GCAGGAGCGG 
AAGATGCAGA AGCTTATGAA GGCAGCCAAG GAGGGCACCA AAGATGGGCT GGAGAAAACC 
AAAGCTGCTG TGAAGAGAGG CGGCTCCTTC ATCAGGACCA GGTCCTTGGT CTCTCAAGAT 
CACAGGTGTT ATTTTGAAGA AGAGCAGAAT TTGTTCATTG ACGTGGACTG CAAGCACCCG 
GAGGCTGTCC TCACCCCAAT GCCCGAGGGC TTGTCTCAGC AGCAGGTTGT GAGAAGATAC 
ATTCTGGGCT CCATTGTTGA GAGCGAGAAG AACTATGTAG ATGCTCTCCG GAGGATTTTG 
GAGCAATATG AGAAGCCGCT GTCTGAGATG GAGCCCCGGC TCCTGAGTGA CAGGAAGCTC 
AGGATGGTTT TCTACCGCGT CAAGGAGATC CTGCAGTGTC ACTCCATGTT CCAGATTGCG 
CTGGCCAGCC GCGTGTCCGA GTGGGATGTC GTGGAAACAA TCGGCGACGT TTTTGTGGCC 
TCGTTCTCCA AGTCCATGGT GCTGGATGCT TACAGCGAGT ACGTGAACAA CTTCAGCACG 
GCCGTGGCTG TCCTCAAGAA GACGTGTGCC ACAAAGCCTG CTTTCCTTGA GTTCTTAAAG 
CTGAGCCAGG ATTCCAGTCC AGACCGAGTC ACACTCCACA GCCTTATGAT GCGGCCCATC 
CAGAGGTTCC CACAGTTCAT CCTCCTACTG CAGGACATGC TGAAGAACAC CGCCAAAGGA 
CATCCTGACA GGCTGCCCCT TCAGATGGCG CTGACGGAGC TCGAGACCCT GGCAGAGAAG 
CTAAACGAGA GAAAAAGAGA TGCCGACCAG CGATGCGAAA TCAAACAAAT AGCAAAAGCC 
ATAAACGAGA GATACCTGAA CAAGCTCCTC AGCAGTGGAA ACCGGTACCT CGTCCGGTCC 
GATGACGTCA TCGAGACGGT TTACAACGAC CGGGGAGAAA TCGTCAAGAC CAAACAACGC 
CGGATCTTCA TGCTGAATGA CGTGCTCATG TGTGCCACCG CCAGCTCACG GAACTCACAT 
GAAAGCCATG CCGTGATGAG CCAGAGGTAC CTGCTCAAGT GGAGTGTCCC TCTAGGTCAC 
GTGGATGTGA TTCAGTACAA TGGAGGCTCT GGCGCCGGGG AGCACTGTCG GCACCACGCT 
GCACACTCAC CTGAGAGCCT GGCCGTGGTG GCCAACGCGA AGCCACACAA AGTTTACATG 
GGGCCAGGGC AGCTGTACCA GGATCTGCAG AACCTACTGC ACGACCTGAA CGTTGTTGGC 
CAGATCTCGC AGCTGATAGG AAACCTCCGA GGGAGCTATC AGAACTTAAA CCAGTCTGTA 
GCCCATGATT GGACGTCGGG CTTACAGCGG CTGATACTGA GGAAAGAAGA TGCGATCAGA 
GCTGCCGACC GCTGCCGGAT CCAGTTACAG CTCCCGGGAA AGCAGGACAA GTCCGGGCGG 
CCCACGTTCT TCACTGCTGT GTTCAATACG CTTACACCAG CCATCAAGGA GTCCTGGGTC 
AGCAGCCTGC AGATGGCCAA GCTGGCCCTT GAGGAGGAGA ACCATATGGG ATGGTTCTGT 
GTGGATGACG ATGGCAACCT AGCCAAGAAG GAGACACACC CTCTCCTGGT GGGACACATG 
CCTGTGATGG TGGCCAAGCA GCCAGAGTTC AAGATTGAAT GTGCAGCTTA TAACCCTGAA 
CCTTACCTGA GCAATGAAAG TCAACCAGCC TCACCCTCCA CAGCCCGTGG CTTTCTGTGG 
ATTGGAAGCT GTAGCAACCA GATGGGTCAG GTCGCCATTG TCTCCTTCCA AGGTTCCAAC 
CCCAAAGTCA TCGAGTGCTT CAATGTGGAA TCACGCATCC TGTGTATGGT ATACATTCCC 
GCAGAGGAGT CAAAGCCCCA AGAAACCACC GAAACCAAGG ATCCAGAAGC CACAGCCTCG 
AGAGCACCCC ATGTGCCCAC CATCTGCCTG GGCACAGAAG AGGGAAGCAT TTCCATTTAC 
AAAAGCAGTC AGGGCTGCAA GAAGGTGAGG CTTCAGCACT TCTATGCACC GGACAAGTCC 
ACGGTCATGA GCTTGGCCTG CTCCCCACAA GGCCTGTATG CAGGCCTGGT CAATGGGTCC 
GTAGCCAGCT ACACCAAGGC TCCAGCACCG AGCTCTCCTG GCAGCCCGTG GGCAGAGTTC 
TGTCTCATCT GCGTTTTCCT GCCATGCTTG CCCTCGGGAG CATATGCCAT CTCGGGTACA 
GGCTGTGGTG CAGTGAAGGG CTATGGATCC TGGAACTCGG AACCCCAGCA AGTGATCAAG 
TTGGGTGTCC TGCCAGTCAG AAGCCTTCTC CTGGTGGAGG GTGCACTGTG GGCAGCCTCG 
GGAGGGCAGG TGTTCATGGC CAGCGTGGAG ACTCACACTA TAGAGAATCA GCTGGAGGCC 
CACCAAGATG AAGGGATGGT CATCTCCCAC ATGGCTGTGG CTGGCGTTGG CATCTGGATT 
GCCTTCACCT CGGGGTCCAC CCTCCGCCTC TTCCACACAG AGACCTTGAA GCACCTGCAG 
GATGTCAACA TTGATGCCCC TGTGCATAGC ATGCTGCCAG GGCACCAGCG TCTATCTGTG 
ACCAGCCTGC TCGTCTGCCA CGGCTTGCTC ATGGTCGGTA CCAGCCTGGG TGTCGTGGTG 
GCCCTGCCAG TCCCTCGGCT TCAGGGTATC CCCAAAGTGA CAGGGCGAGG CATGGTCTCC 
TACCATGCGC ATAACGGGCC CGTTAAGTTC ATCGTCTCAG CCACGGCGTT TCAAAACAAG 
GACAGAGCCA GGGACAGCCC ACGTTCCGGT TCTGAGCTTC AGGATGAAGA CCCCAAGGAC 
TTGCTCTGCA GCGAGGAAGG TCCTTCCTGC CCGGGCCAGC CTGACACCTA CACATCCGTT 
TGGTTGGGAG ACTCCCTAGG ATTGCCAACT CAAAAGAATG ATCTGTCTTC GTCCTCGGGG 
TCACTGAACC TGTCCCATGG CTCCAGTTCC CTAGAGCACA GATCTGTGGA CAGCAGTCTG 
TGTGACCTGC TGAGGGACCC CAGTGCCTCC CCAAGGAGCA GACCACAGGG CTCTAGGCGA 
GCAAGAGCCA GTTCAGCGCT GGTGGTATGT GGGGGCCAGG GCCACCGGCG GGTGCACAGG 
AAGGCCAGGC AGCCTTCTCA GGAGGACCTG GTTTCTTCAG TGATGGTGTG GCAGATCCCC 
CTGCTGGGCA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168162.1
CDS	189..4343
Translation	MEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQLSHDLTRFKAHCEEKMRGLVASTVGAMEIQQAKQRQERKMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPAPSSPGSPWAEFCLICVFLPCLPSGAYAISGTGCGAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_017312673.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312673.1

ORF Insert Sequence:

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGCA GTGAGCAAGG CAGCCAAGAA GGCAGTGCCC TGGCCCGATG GGCAGCCGAC 
CCTGCCAACA CAGCATGGAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG 
GAAAACTCGG ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA 
GGTGGAAACA GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG 
CCAAGCGACT CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA 
AAGCAACTCT CTCATGACCT AACCCGCTTT AAGGCGCACT GTGAGGAAAA GATGAGAGGC 
TTGGTGGCCA GTACGGTGGG AGCCATGGAG ATTCAGCAGG CAAAGCAGAG GCAGGAGCGG 
AAGATGCAGA AGCTTATGAA GGCAGCCAAG GAGGGCACCA AAGATGGGCT GGAGAAAACC 
AAAGCTGCTG TGAAGAGAGG CGGCTCCTTC ATCAGGACCA GGTCCTTGGT CTCTCAAGAT 
CACAGGTGTT ATTTTGAAGA AGAGCAGAAT TTGTTCATTG ACGTGGACTG CAAGCACCCG 
GAGGCTGTCC TCACCCCAAT GCCCGAGGGC TTGTCTCAGC AGCAGGTTGT GAGAAGATAC 
ATTCTGGGCT CCATTGTTGA GAGCGAGAAG AACTATGTAG ATGCTCTCCG GAGGATTTTG 
GAGCAATATG AGAAGCCGCT GTCTGAGATG GAGCCCCGGC TCCTGAGTGA CAGGAAGCTC 
AGGATGGTTT TCTACCGCGT CAAGGAGATC CTGCAGTGTC ACTCCATGTT CCAGATTGCG 
CTGGCCAGCC GCGTGTCCGA GTGGGATGTC GTGGAAACAA TCGGCGACGT TTTTGTGGCC 
TCGTTCTCCA AGTCCATGGT GCTGGATGCT TACAGCGAGT ACGTGAACAA CTTCAGCACG 
GCCGTGGCTG TCCTCAAGAA GACGTGTGCC ACAAAGCCTG CTTTCCTTGA GTTCTTAAAG 
CTGAGCCAGG ATTCCAGTCC AGACCGAGTC ACACTCCACA GCCTTATGAT GCGGCCCATC 
CAGAGGTTCC CACAGTTCAT CCTCCTACTG CAGGACATGC TGAAGAACAC CGCCAAAGGA 
CATCCTGACA GGCTGCCCCT TCAGATGGCG CTGACGGAGC TCGAGACCCT GGCAGAGAAG 
CTAAACGAGA GAAAAAGAGA TGCCGACCAG CGATGCGAAA TCAAACAAAT AGCAAAAGCC 
ATAAACGAGA GATACCTGAA CAAGCTCCTC AGCAGTGGAA ACCGGTACCT CGTCCGGTCC 
GATGACGTCA TCGAGACGGT TTACAACGAC CGGGGAGAAA TCGTCAAGAC CAAACAACGC 
CGGATCTTCA TGCTGAATGA CGTGCTCATG TGTGCCACCG CCAGCTCACG GAACTCACAT 
GAAAGCCATG CCGTGATGAG CCAGAGGTAC CTGCTCAAGT GGAGTGTCCC TCTAGGTCAC 
GTGGATGTGA TTCAGTACAA TGGAGGCTCT GGCGCCGGGG AGCACTGTCG GCACCACGCT 
GCACACTCAC CTGAGAGCCT GGCCGTGGTG GCCAACGCGA AGCCACACAA AGTTTACATG 
GGGCCAGGGC AGCTGTACCA GGATCTGCAG AACCTACTGC ACGACCTGAA CGTTGTTGGC 
CAGATCTCGC AGCTGATAGG AAACCTCCGA GGGAGCTATC AGAACTTAAA CCAGTCTGTA 
GCCCATGATT GGACGTCGGG CTTACAGCGG CTGATACTGA GGAAAGAAGA TGCGATCAGA 
GCTGCCGACC GCTGCCGGAT CCAGTTACAG CTCCCGGGAA AGCAGGACAA GTCCGGGCGG 
CCCACGTTCT TCACTGCTGT GTTCAATACG CTTACACCAG CCATCAAGGA GTCCTGGGTC 
AGCAGCCTGC AGATGGCCAA GCTGGCCCTT GAGGAGGAGA ACCATATGGG ATGGTTCTGT 
GTGGATGACG ATGGCAACCT AGCCAAGAAG GAGACACACC CTCTCCTGGT GGGACACATG 
CCTGTGATGG TGGCCAAGCA GCCAGAGTTC AAGATTGAAT GTGCAGCTTA TAACCCTGAA 
CCTTACCTGA GCAATGAAAG TCAACCAGCC TCACCCTCCA CAGCCCGTGG CTTTCTGTGG 
ATTGGAAGCT GTAGCAACCA GATGGGTCAG GTCGCCATTG TCTCCTTCCA AGGTTCCAAC 
CCCAAAGTCA TCGAGTGCTT CAATGTGGAA TCACGCATCC TGTGTATGGT ATACATTCCC 
GCAGAGGAGT CAAAGCCCCA AGAAACCACC GAAACCAAGG ATCCAGAAGC CACAGCCTCG 
AGAGCACCCC ATGTGCCCAC CATCTGCCTG GGCACAGAAG AGGGAAGCAT TTCCATTTAC 
AAAAGCAGTC AGGGCTGCAA GAAGGTGAGG CTTCAGCACT TCTATGCACC GGACAAGTCC 
ACGGTCATGA GCTTGGCCTG CTCCCCACAA GGCCTGTATG CAGGCCTGGT CAATGGGTCC 
GTAGCCAGCT ACACCAAGGC TCCAGCACCG AGCTCTCCTG GCAGCCCGTG GGCAGAGTTC 
TGTCTCATCT GCGTTTTCCT GCCATGCTTG CCCTCGGGAG CATATGCCAT CTCGGGTACA 
GGCTGTGGTG CAGTGAAGGG CTATGGATCC TGGAACTCGG AACCCCAGCA AGTGATCAAG 
TTGGGTGTCC TGCCAGTCAG AAGCCTTCTC CTGGTGGAGG GTGCACTGTG GGCAGCCTCG 
GGAGGGCAGG TGTTCATGGC CAGCGTGGAG ACTCACACTA TAGAGAATCA GCTGGAGGCC 
CACCAAGATG AAGGGATGGT CATCTCCCAC ATGGCTGTGG CTGGCGTTGG CATCTGGATT 
GCCTTCACCT CGGGGTCCAC CCTCCGCCTC TTCCACACAG AGACCTTGAA GCACCTGCAG 
GATGTCAACA TTGATGCCCC TGTGCATAGC ATGCTGCCAG GGCACCAGCG TCTATCTGTG 
ACCAGCCTGC TCGTCTGCCA CGGCTTGCTC ATGGTCGGTA CCAGCCTGGG TGTCGTGGTG 
GCCCTGCCAG TCCCTCGGCT TCAGGGTATC CCCAAAGTGA CAGGGCGAGG CATGGTCTCC 
TACCATGCGC ATAACGGGCC CGTTAAGTTC ATCGTCTCAG CCACGGCGTT TCAAAACAAG 
GACAGAGCCA GGGACAGCCC ACGTTCCGGT TCTGAGCTTC AGGATGAAGA CCCCAAGGAC 
TTGCTCTGCA GCGAGGAAGG TCCTTCCTGC CCGGGCCAGC CTGACACCTA CACATCCGTT 
TGGTTGGGAG ACTCCCTAGG ATTGCCAACT CAAAAGAATG ATCTGTCTTC GTCCTCGGGG 
TCACTGAACC TGTCCCATGG CTCCAGTTCC CTAGAGCACA GATCTGTGGA CAGCAGTCTG 
TGTGACCTGC TGAGGGACCC CAGTGCCTCC CCAAGGAGCA GACCACAGGG CTCTAGGCGA 
GCAAGAGCCA GTTCAGCGCT GGTGGTATGT GGGGGCCAGG GCCACCGGCG GGTGCACAGG 
AAGGCCAGGC AGCCTTCTCA GGAGGACCTG GTTTCTTCAG TGATGGTGTG GCAGATCCCC 
CTGCTGGGCA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef10 ( XM_006508772.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508835.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X11, mRNA.

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CloneID	OMu40385
Clone ID Related Accession (Same CDS sequence)	XM_006508772.3
Accession Version	XM_006508772.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 10 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508772.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGTG AGCAAGGCAG CCAAGAAGGC AGTGCCCTGG CCCGATGGGC AGCCGACCCT 
GCCAACACAG CATGGATGGA AAATCCAGAG GAAGCAATTT ACGACGACGT TCCGAGGGAA 
AACTCGGACT CTGAGCCAGA TGAAATGATT TATGATGATG TGGAGAACGG CGAAGAAGGT 
GGAAACAGCT CCCCAGAGTA CGGGTGGAGT TCCAGCGAAT TCGAAAGCTA TGAGGAGCCA 
AGCGACTCGG AGGGCAAGAA CGGAATTCCC AGGTCCTTCC TGCGCAGCAG TCATAAAAAG 
CAACTCTCTC ATGACCTAAC CCGCTTTAAG GCGCACTGTG AGGAAAAGAT GAGAGGCTTG 
GTGGCCAGTA CGGTGGGAGC CATGGAGATT CAGCAGGCAA AGCAGAGGCA GGAGCGGAAG 
ATGCAGAAGC TTATGAAGGC AGCCAAGGAG GGCACCAAAG ATGGGCTGGA GAAAACCAAA 
GCTGCTGTGA AGAGAGGCGG CTCCTTCATC AGGACCAGGT CCTTGGTCTC TCAAGATCAC 
AGGTGTTATT TTGAAGAAGA GCAGAATTTG TTCATTGACG TGGACTGCAA GCACCCGGAG 
GCTGTCCTCA CCCCAATGCC CGAGGGCTTG TCTCAGCAGC AGGTTGTGAG AAGATACATT 
CTGGGCTCCA TTGTTGAGAG CGAGAAGAAC TATGTAGATG CTCTCCGGAG GATTTTGGAG 
CAATATGAGA AGCCGCTGTC TGAGATGGAG CCCCGGCTCC TGAGTGACAG GAAGCTCAGG 
ATGGTTTTCT ACCGCGTCAA GGAGATCCTG CAGTGTCACT CCATGTTCCA GATTGCGCTG 
GCCAGCCGCG TGTCCGAGTG GGATGTCGTG GAAACAATCG GCGACGTTTT TGTGGCCTCG 
TTCTCCAAGT CCATGGTGCT GGATGCTTAC AGCGAGTACG TGAACAACTT CAGCACGGCC 
GTGGCTGTCC TCAAGAAGAC GTGTGCCACA AAGCCTGCTT TCCTTGAGTT CTTAAAGCTG 
AGCCAGGATT CCAGTCCAGA CCGAGTCACA CTCCACAGCC TTATGATGCG GCCCATCCAG 
AGGTTCCCAC AGTTCATCCT CCTACTGCAG GACATGCTGA AGAACACCGC CAAAGGACAT 
CCTGACAGGC TGCCCCTTCA GATGGCGCTG ACGGAGCTCG AGACCCTGGC AGAGAAGCTA 
AACGAGAGAA AAAGAGATGC CGACCAGCGA TGCGAAATCA AACAAATAGC AAAAGCCATA 
AACGAGAGAT ACCTGAACAA GCTCCTCAGC AGTGGAAACC GGTACCTCGT CCGGTCCGAT 
GACGTCATCG AGACGGTTTA CAACGACCGG GGAGAAATCG TCAAGACCAA ACAACGCCGG 
ATCTTCATGC TGAATGACGT GCTCATGTGT GCCACCGCCA GCTCACGGAA CTCACATGAA 
AGCCATGCCG TGATGAGCCA GAGGTACCTG CTCAAGTGGA GTGTCCCTCT AGGTCACGTG 
GATGTGATTC AGTACAATGG AGGCTCTGGC GCCGGGGAGC ACTGTCGGCA CCACGCTGCA 
CACTCACCTG AGAGCCTGGC CGTGGTGGCC AACGCGAAGC CACACAAAGT TTACATGGGG 
CCAGGGCAGC TGTACCAGGA TCTGCAGAAC CTACTGCACG ACCTGAACGT TGTTGGCCAG 
ATCTCGCAGC TGATAGGAAA CCTCCGAGGG AGCTATCAGA ACTTAAACCA GTCTGTAGCC 
CATGATTGGA CGTCGGGCTT ACAGCGGCTG ATACTGAGGA AAGAAGATGC GATCAGAGCT 
GCCGACCGCT GCCGGATCCA GTTACAGCTC CCGGGAAAGC AGGACAAGTC CGGGCGGCCC 
ACGTTCTTCA CTGCTGTGTT CAATACGCTT ACACCAGCCA TCAAGGAGTC CTGGGTCAGC 
AGCCTGCAGA TGGCCAAGCT GGCCCTTGAG GAGGAGAACC ATATGGGATG GTTCTGTGTG 
GATGACGATG GCAACCTAGC CAAGAAGGAG ACACACCCTC TCCTGGTGGG ACACATGCCT 
GTGATGGTGG CCAAGCAGCC AGAGTTCAAG ATTGAATGTG CAGCTTATAA CCCTGAACCT 
TACCTGAGCA ATGAAAGTCA ACCAGCCTCA CCCTCCACAG CCCGTGGCTT TCTGTGGATT 
GGAAGCTGTA GCAACCAGAT GGGTCAGGTC GCCATTGTCT CCTTCCAAGG TTCCAACCCC 
AAAGTCATCG AGTGCTTCAA TGTGGAATCA CGCATCCTGT GTATGGTATA CATTCCCGCA 
GAGGAGTCAA AGCCCCAAGA AACCACCGAA ACCAAGGATC CAGAAGCCAC AGCCTCGAGA 
GCACCCCATG TGCCCACCAT CTGCCTGGGC ACAGAAGAGG GAAGCATTTC CATTTACAAA 
AGCAGTCAGG GCTGCAAGAA GGTGAGGCTT CAGCACTTCT ATGCACCGGA CAAGTCCACG 
GTCATGAGCT TGGCCTGCTC CCCACAAGGC CTGTATGCAG GCCTGGTCAA TGGGTCCGTA 
GCCAGCTACA CCAAGGCTCC AGCACCGAGC TCTCCTGGCA GCCCGTGGGC AGAGTTCTGT 
CTCATCTGCG TTTTCCTGCC ATGCTTGCCC TCGGGAGCAT ATGCCATCTC GGGTACAGGC 
TGTGGTGCAG TGAAGGGCTA TGGATCCTGG AACTCGGAAC CCCAGCAAGT GATCAAGTTG 
GGTGTCCTGC CAGTCAGAAG CCTTCTCCTG GTGGAGGGTG CACTGTGGGC AGCCTCGGGA 
GGGCAGGTGT TCATGGCCAG CGTGGAGACT CACACTATAG AGAATCAGCT GGAGGCCCAC 
CAAGATGAAG GGATGGTCAT CTCCCACATG GCTGTGGCTG GCGTTGGCAT CTGGATTGCC 
TTCACCTCGG GGTCCACCCT CCGCCTCTTC CACACAGAGA CCTTGAAGCA CCTGCAGGAT 
GTCAACATTG ATGCCCCTGT GCATAGCATG CTGCCAGGGC ACCAGCGTCT ATCTGTGACC 
AGCCTGCTCG TCTGCCACGG CTTGCTCATG GTCGGTACCA GCCTGGGTGT CGTGGTGGCC 
CTGCCAGTCC CTCGGCTTCA GGGTATCCCC AAAGTGACAG GGCGAGGCAT GGTCTCCTAC 
CATGCGCATA ACGGGCCCGT TAAGTTCATC GTCTCAGCCA CGGCGTTTCA AAACAAGGAC 
AGAGCCAGGG ACAGCCCACG TTCCGGTTCT GAGCTTCAGG ATGAAGACCC CAAGGACTTG 
CTCTGCAGCG AGGAAGGTCC TTCCTGCCCG GGCCAGCCTG ACACCTACAC ATCCGTTTGG 
TTGGGAGACT CCCTAGGATT GCCAACTCAA AAGAATGATC TGTCTTCGTC CTCGGGGTCA 
CTGAACCTGT CCCATGGCTC CAGTTCCCTA GAGCACAGAT CTGTGGACAG CAGTCTGTGT 
GACCTGCTGA GGGACCCCAG TGCCTCCCCA AGGAGCAGAC CACAGGGCTC TAGGCGAGCA 
AGAGCCAGTT CAGCGCTGGT GGTATGTGGG GGCCAGGGCC ACCGGCGGGT GCACAGGAAG 
GCCAGGCAGC CTTCTCAGGA GGACCTGGTT TCTTCAGTGA TGGTGTGGCA GATCCCCCTG 
CTGGGCATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508835.1
CDS	236..4387
Translation	MEQGEASPPVPAEHEAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEAEPAKLVVPTKVNPYSVIDITPLQEDQPSSPDANTEEEGVGLRVPSGYSVPVPCGYAVPSNLPLLLPAYSSPVIIRAESVEEEEAAETVGDGQCNSLSSEDLPHSEQGSQEGSALARWAADPANTAWMENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLRSSHKKQLSHDLTRFKAHCEEKMRGLVASTVGAMEIQQAKQRQERKMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDSSPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECAAYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESKPQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAGLVNGSVASYTKAPAPSSPGSPWAEFCLICVFLPCLPSGAYAISGTGCGAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDEGMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGTSLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM

Target ORF information:

RefSeq Version	XM_006508772.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508772.3

ORF Insert Sequence:

ATGGAGCAGG GAGAGGCCTC ACCTCCTGTT CCTGCAGAAC ATGAAGCGAA ATGTGACACC 
AGCAACAACG AAGAGGAGGG AGAACTGTTT GACTTCGACA GTGGAGATGA AGTTCCCGAA 
GCGGATCGGC AAGTCCCGTC TGCCGACGAT AGGACGAGAG GTGCTGAAGC AGGAGGTGCA 
GACGAGAACA CGTGCCCTGC AGGAAATGGA ACTGGAGCTG AACCTGCCCC CGAGGCAGAG 
CCTGCGAAAC TTGTGGTCCC CACAAAAGTG AACCCGTATT CTGTCATCGA CATCACGCCG 
CTGCAGGAAG ACCAGCCGTC ATCTCCAGAC GCGAATACAG AGGAGGAAGG CGTGGGGCTG 
CGTGTGCCCA GTGGTTACTC TGTGCCTGTG CCCTGTGGCT ATGCTGTCCC CTCCAACCTG 
CCATTGCTGC TGCCTGCCTA CTCCAGTCCT GTCATCATCC GAGCCGAGTC TGTAGAGGAG 
GAAGAAGCAG CAGAAACTGT GGGAGATGGG CAGTGCAATT CCCTAAGCTC CGAGGACCTT 
CCACACAGTG AGCAAGGCAG CCAAGAAGGC AGTGCCCTGG CCCGATGGGC AGCCGACCCT 
GCCAACACAG CATGGATGGA AAATCCAGAG GAAGCAATTT ACGACGACGT TCCGAGGGAA 
AACTCGGACT CTGAGCCAGA TGAAATGATT TATGATGATG TGGAGAACGG CGAAGAAGGT 
GGAAACAGCT CCCCAGAGTA CGGGTGGAGT TCCAGCGAAT TCGAAAGCTA TGAGGAGCCA 
AGCGACTCGG AGGGCAAGAA CGGAATTCCC AGGTCCTTCC TGCGCAGCAG TCATAAAAAG 
CAACTCTCTC ATGACCTAAC CCGCTTTAAG GCGCACTGTG AGGAAAAGAT GAGAGGCTTG 
GTGGCCAGTA CGGTGGGAGC CATGGAGATT CAGCAGGCAA AGCAGAGGCA GGAGCGGAAG 
ATGCAGAAGC TTATGAAGGC AGCCAAGGAG GGCACCAAAG ATGGGCTGGA GAAAACCAAA 
GCTGCTGTGA AGAGAGGCGG CTCCTTCATC AGGACCAGGT CCTTGGTCTC TCAAGATCAC 
AGGTGTTATT TTGAAGAAGA GCAGAATTTG TTCATTGACG TGGACTGCAA GCACCCGGAG 
GCTGTCCTCA CCCCAATGCC CGAGGGCTTG TCTCAGCAGC AGGTTGTGAG AAGATACATT 
CTGGGCTCCA TTGTTGAGAG CGAGAAGAAC TATGTAGATG CTCTCCGGAG GATTTTGGAG 
CAATATGAGA AGCCGCTGTC TGAGATGGAG CCCCGGCTCC TGAGTGACAG GAAGCTCAGG 
ATGGTTTTCT ACCGCGTCAA GGAGATCCTG CAGTGTCACT CCATGTTCCA GATTGCGCTG 
GCCAGCCGCG TGTCCGAGTG GGATGTCGTG GAAACAATCG GCGACGTTTT TGTGGCCTCG 
TTCTCCAAGT CCATGGTGCT GGATGCTTAC AGCGAGTACG TGAACAACTT CAGCACGGCC 
GTGGCTGTCC TCAAGAAGAC GTGTGCCACA AAGCCTGCTT TCCTTGAGTT CTTAAAGCTG 
AGCCAGGATT CCAGTCCAGA CCGAGTCACA CTCCACAGCC TTATGATGCG GCCCATCCAG 
AGGTTCCCAC AGTTCATCCT CCTACTGCAG GACATGCTGA AGAACACCGC CAAAGGACAT 
CCTGACAGGC TGCCCCTTCA GATGGCGCTG ACGGAGCTCG AGACCCTGGC AGAGAAGCTA 
AACGAGAGAA AAAGAGATGC CGACCAGCGA TGCGAAATCA AACAAATAGC AAAAGCCATA 
AACGAGAGAT ACCTGAACAA GCTCCTCAGC AGTGGAAACC GGTACCTCGT CCGGTCCGAT 
GACGTCATCG AGACGGTTTA CAACGACCGG GGAGAAATCG TCAAGACCAA ACAACGCCGG 
ATCTTCATGC TGAATGACGT GCTCATGTGT GCCACCGCCA GCTCACGGAA CTCACATGAA 
AGCCATGCCG TGATGAGCCA GAGGTACCTG CTCAAGTGGA GTGTCCCTCT AGGTCACGTG 
GATGTGATTC AGTACAATGG AGGCTCTGGC GCCGGGGAGC ACTGTCGGCA CCACGCTGCA 
CACTCACCTG AGAGCCTGGC CGTGGTGGCC AACGCGAAGC CACACAAAGT TTACATGGGG 
CCAGGGCAGC TGTACCAGGA TCTGCAGAAC CTACTGCACG ACCTGAACGT TGTTGGCCAG 
ATCTCGCAGC TGATAGGAAA CCTCCGAGGG AGCTATCAGA ACTTAAACCA GTCTGTAGCC 
CATGATTGGA CGTCGGGCTT ACAGCGGCTG ATACTGAGGA AAGAAGATGC GATCAGAGCT 
GCCGACCGCT GCCGGATCCA GTTACAGCTC CCGGGAAAGC AGGACAAGTC CGGGCGGCCC 
ACGTTCTTCA CTGCTGTGTT CAATACGCTT ACACCAGCCA TCAAGGAGTC CTGGGTCAGC 
AGCCTGCAGA TGGCCAAGCT GGCCCTTGAG GAGGAGAACC ATATGGGATG GTTCTGTGTG 
GATGACGATG GCAACCTAGC CAAGAAGGAG ACACACCCTC TCCTGGTGGG ACACATGCCT 
GTGATGGTGG CCAAGCAGCC AGAGTTCAAG ATTGAATGTG CAGCTTATAA CCCTGAACCT 
TACCTGAGCA ATGAAAGTCA ACCAGCCTCA CCCTCCACAG CCCGTGGCTT TCTGTGGATT 
GGAAGCTGTA GCAACCAGAT GGGTCAGGTC GCCATTGTCT CCTTCCAAGG TTCCAACCCC 
AAAGTCATCG AGTGCTTCAA TGTGGAATCA CGCATCCTGT GTATGGTATA CATTCCCGCA 
GAGGAGTCAA AGCCCCAAGA AACCACCGAA ACCAAGGATC CAGAAGCCAC AGCCTCGAGA 
GCACCCCATG TGCCCACCAT CTGCCTGGGC ACAGAAGAGG GAAGCATTTC CATTTACAAA 
AGCAGTCAGG GCTGCAAGAA GGTGAGGCTT CAGCACTTCT ATGCACCGGA CAAGTCCACG 
GTCATGAGCT TGGCCTGCTC CCCACAAGGC CTGTATGCAG GCCTGGTCAA TGGGTCCGTA 
GCCAGCTACA CCAAGGCTCC AGCACCGAGC TCTCCTGGCA GCCCGTGGGC AGAGTTCTGT 
CTCATCTGCG TTTTCCTGCC ATGCTTGCCC TCGGGAGCAT ATGCCATCTC GGGTACAGGC 
TGTGGTGCAG TGAAGGGCTA TGGATCCTGG AACTCGGAAC CCCAGCAAGT GATCAAGTTG 
GGTGTCCTGC CAGTCAGAAG CCTTCTCCTG GTGGAGGGTG CACTGTGGGC AGCCTCGGGA 
GGGCAGGTGT TCATGGCCAG CGTGGAGACT CACACTATAG AGAATCAGCT GGAGGCCCAC 
CAAGATGAAG GGATGGTCAT CTCCCACATG GCTGTGGCTG GCGTTGGCAT CTGGATTGCC 
TTCACCTCGG GGTCCACCCT CCGCCTCTTC CACACAGAGA CCTTGAAGCA CCTGCAGGAT 
GTCAACATTG ATGCCCCTGT GCATAGCATG CTGCCAGGGC ACCAGCGTCT ATCTGTGACC 
AGCCTGCTCG TCTGCCACGG CTTGCTCATG GTCGGTACCA GCCTGGGTGT CGTGGTGGCC 
CTGCCAGTCC CTCGGCTTCA GGGTATCCCC AAAGTGACAG GGCGAGGCAT GGTCTCCTAC 
CATGCGCATA ACGGGCCCGT TAAGTTCATC GTCTCAGCCA CGGCGTTTCA AAACAAGGAC 
AGAGCCAGGG ACAGCCCACG TTCCGGTTCT GAGCTTCAGG ATGAAGACCC CAAGGACTTG 
CTCTGCAGCG AGGAAGGTCC TTCCTGCCCG GGCCAGCCTG ACACCTACAC ATCCGTTTGG 
TTGGGAGACT CCCTAGGATT GCCAACTCAA AAGAATGATC TGTCTTCGTC CTCGGGGTCA 
CTGAACCTGT CCCATGGCTC CAGTTCCCTA GAGCACAGAT CTGTGGACAG CAGTCTGTGT 
GACCTGCTGA GGGACCCCAG TGCCTCCCCA AGGAGCAGAC CACAGGGCTC TAGGCGAGCA 
AGAGCCAGTT CAGCGCTGGT GGTATGTGGG GGCCAGGGCC ACCGGCGGGT GCACAGGAAG 
GCCAGGCAGC CTTCTCAGGA GGACCTGGTT TCTTCAGTGA TGGTGTGGCA GATCCCCCTG 
CTGGGCATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Arhgef10
Entrez Gene ID	234094
Full Name	Rho guanine nucleotide exchange factor (GEF) 10
Synonyms	6430549H08Rik,mKIAA0294
General protein information	Preferred Names Rho guanine nucleotide exchange factor (GEF) 10 Names rho guanine nucleotide exchange factor 10
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A1.1

Rattus norvegicus (Norway rat)	Arhgef10	XP_006253464.1
	LOC722745	XP_002805294.1
Gallus gallus (chicken)	ARHGEF10	XP_001235643.3
Homo sapiens (human)	ARHGEF10	NP_055444.2
Canis lupus familiaris (dog)	ARHGEF10	XP_005630094.1
Mus musculus (house mouse)	Arhgef10	NP_766339.2
Xenopus tropicalis (tropical clawed frog)	arhgef10	XP_002935203.2
Bos taurus (cattle)	ARHGEF10	XP_005225971.1
Danio rerio (zebrafish)	arhgef10	XP_005158599.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Arhgef10 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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