Mrgprb4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mrgprb4
Entrez Gene ID	233230
Full Name	MAS-related GPR, member B4
Synonyms	MrgB4
General protein information	Preferred Names MAS-related GPR, member B4 Names mas-related G-protein coupled receptor member B4 MrgB4 G protein-coupled receptor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_205795.1	NP_991364.1	mas-related G-protein coupled receptor member B4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mrgprb4	NP_991364.1
Mus musculus (house mouse)	Mrgprb5	NP_997421.1
Rattus norvegicus (Norway rat)	Mrgprb5	NP_001002284.1
Rattus norvegicus (Norway rat)	Mrgprb4	NP_001002287.1

Gene Ontology
Bibliography

Related articles in PubMed

p75 Is Required for the Establishment of Postnatal Sensory Neuron Diversity by Potentiating Ret Signaling.
Chen Z, Donnelly CR, Dominguez B, Harada Y, Lin W, Halim AS, Bengoechea TG, Pierchala BA, Lee KF
Cell reports21(3)707-720(2017 Oct)

A mechanically activated ion channel is functionally expressed in the MrgprB4 positive sensory neurons, which detect stroking of hairy skin in mice.
Yamaguchi S, Otsuguro KI
Neuroscience letters653139-145(2017 Jul)

Incoherent feed-forward regulatory loops control segregation of C-mechanoreceptors, nociceptors, and pruriceptors.
Lou S, Pan X, Huang T, Duan B, Yang FC, Yang J, Xiong M, Liu Y, Ma Q
The Journal of neuroscience : the official journal of the Society for Neuroscience35(13)5317-29(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Mas-related G protein-coupled receptor B4 marks a rare subpopulation of unmyelinated, nonpeptidergic sensory fibers that exclusively innervate hairy skin.
Title: Molecular genetic visualization of a rare subset of unmyelinated sensory neurons that may detect gentle touch.

The following Mrgprb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mrgprb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58120	NM_205795.1 Latest version!	Mus musculus MAS-related GPR, member B4 (Mrgprb4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mrgprb4 ( NM_205795.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_991364.1 Mus musculus MAS-related GPR, member B4 (Mrgprb4), mRNA.

Price & Availability↑

CloneID	OMu58120
Clone ID Related Accession (Same CDS sequence)	NM_205795.1
Accession Version	NM_205795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Mus musculus(house mouse)
Product	mas-related G-protein coupled receptor member B4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY042202.1. On Mar 22, 2004 this sequence version replaced XM_145609.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC107202.1 [ECO:0000345] ##Evidence-Data-END##

ATGGGTACAA CCACCCTGGC CTGGAACATT AACAACACCG CTGAAAATGG AAGTTACACT 
GAAATGTTCT CCTGTATCAC CAAGTTCAAT ACCCTGAATT TTCTTACTGT CATCATAGCT 
GTGGTTGGCC TGGCAGGAAA CGGCATAGTG CTATGGCTTC TAGCCTTCCA CCTGCATAGG 
AATGCCTTCT CTGTCTATGT CCTCAATCTG GCTGGTGCTG ATTTCTTGTA CCTTTTCACT 
CAAGTTGTGC ATTCCCTGGA ATGTGTCCTT CAGTTAGATA ATAACTCCTT TTATATTCTC 
CTCATTGTAA CAATGTTTGC TTACCTTGCA GGTTTGTGTA TGATTGCAGC CATCAGTGCT 
GAACGCTGCC TATCTGTTAT GTGGCCTATC TGGTATCACT GCCAAAGACC AAGACACACA 
TCAGCCATCA TGTGTGCTCT GGTCTGGGTT TCCTCTCTAT TGTTGAGCCT CGTGGTAGGG 
CTAGGCTGTG GTTTTCTGTT CAGTTATTAT GATTATTATT TCTGTATTAC TTTGAATTTT 
ATCACTGCTG CATTTTTAAT AGTGTTATCT GTGGTTCTTT CTGTATCTAG CCTGGCCCTG 
TTGGTGAAGA TTGTGTGGGG GTCACACAGG ATTCCTGTGA CCAGGTTCTT TGTGACCATT 
GCTCTCACAG TGGTGGTCTT CATATACTTT GGCATGCCCT TTGGTATCTG CTGGTTCCTC 
TTATCAAGGA TTATGGAGTT TGATAGCATT TTCTTTAACA ATGTTTATGA AATAATAGAA 
TTCCTGTCCT GTGTTAACAG CTGTGCCAAT CCCATCATTT ACTTCCTTGT TGGCTCCATT 
AGACAACACA GGTTGCGATG GCAGTCTCTG AAGCTACTTC TTCAGAGAGC CATGCAGGAC 
ACTCCTGAGG AAGAGAGTGG AGAGAGGGGT CCTTCGCAAA GGTCTGGGGA ACTGGAAACA 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991364.1
CDS	1..966
Translation	MGTTTLAWNINNTAENGSYTEMFSCITKFNTLNFLTVIIAVVGLAGNGIVLWLLAFHLHRNAFSVYVLNLAGADFLYLFTQVVHSLECVLQLDNNSFYILLIVTMFAYLAGLCMIAAISAERCLSVMWPIWYHCQRPRHTSAIMCALVWVSSLLLSLVVGLGCGFLFSYYDYYFCITLNFITAAFLIVLSVVLSVSSLALLVKIVWGSHRIPVTRFFVTIALTVVVFIYFGMPFGICWFLLSRIMEFDSIFFNNVYEIIEFLSCVNSCANPIIYFLVGSIRQHRLRWQSLKLLLQRAMQDTPEEESGERGPSQRSGELETV

Target ORF information:

RefSeq Version	NM_205795.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAS-related GPR, member B4 (Mrgprb4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205795.1

ORF Insert Sequence:

ATGGGTACAA CCACCCTGGC CTGGAACATT AACAACACCG CTGAAAATGG AAGTTACACT 
GAAATGTTCT CCTGTATCAC CAAGTTCAAT ACCCTGAATT TTCTTACTGT CATCATAGCT 
GTGGTTGGCC TGGCAGGAAA CGGCATAGTG CTATGGCTTC TAGCCTTCCA CCTGCATAGG 
AATGCCTTCT CTGTCTATGT CCTCAATCTG GCTGGTGCTG ATTTCTTGTA CCTTTTCACT 
CAAGTTGTGC ATTCCCTGGA ATGTGTCCTT CAGTTAGATA ATAACTCCTT TTATATTCTC 
CTCATTGTAA CAATGTTTGC TTACCTTGCA GGTTTGTGTA TGATTGCAGC CATCAGTGCT 
GAACGCTGCC TATCTGTTAT GTGGCCTATC TGGTATCACT GCCAAAGACC AAGACACACA 
TCAGCCATCA TGTGTGCTCT GGTCTGGGTT TCCTCTCTAT TGTTGAGCCT CGTGGTAGGG 
CTAGGCTGTG GTTTTCTGTT CAGTTATTAT GATTATTATT TCTGTATTAC TTTGAATTTT 
ATCACTGCTG CATTTTTAAT AGTGTTATCT GTGGTTCTTT CTGTATCTAG CCTGGCCCTG 
TTGGTGAAGA TTGTGTGGGG GTCACACAGG ATTCCTGTGA CCAGGTTCTT TGTGACCATT 
GCTCTCACAG TGGTGGTCTT CATATACTTT GGCATGCCCT TTGGTATCTG CTGGTTCCTC 
TTATCAAGGA TTATGGAGTT TGATAGCATT TTCTTTAACA ATGTTTATGA AATAATAGAA 
TTCCTGTCCT GTGTTAACAG CTGTGCCAAT CCCATCATTT ACTTCCTTGT TGGCTCCATT 
AGACAACACA GGTTGCGATG GCAGTCTCTG AAGCTACTTC TTCAGAGAGC CATGCAGGAC 
ACTCCTGAGG AAGAGAGTGG AGAGAGGGGT CCTTCGCAAA GGTCTGGGGA ACTGGAAACA 
GTCTAG