Siglecf cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Siglecf
Entrez Gene ID	233186
Full Name	sialic acid binding Ig-like lectin F
Synonyms	Siglec5,mSiglec-F
General protein information	Preferred Names sialic acid binding Ig-like lectin F Names sialic acid-binding Ig-like lectin 5 sialic acid-binding lectin Siglec-F siglec-5 siglec-F
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271019.1	NP_001257948.1	sialic acid-binding Ig-like lectin 5 isoform 2 precursor
XM_006540816.1	XP_006540879.1	sialic acid-binding Ig-like lectin 5 isoform X1
NM_145581.2	NP_663556.1	sialic acid-binding Ig-like lectin 5 isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	SIGLEC8	NP_055257.2
Mus musculus (house mouse)	Siglec5	NP_663556.1
Rattus norvegicus (Norway rat)	Siglec5	NP_001099719.2
Canis lupus familiaris (dog)	SIGLEC12	XP_005616307.1
Bos taurus (cattle)	LOC618463	XP_002695268.2

Gene Ontology
Bibliography

Related articles in PubMed

Siglec-F is a novel intestinal M cell marker.
Gicheva N, Macauley MS, Arlian BM, Paulson JC, Kawasaki N
Biochemical and biophysical research communications479(1)1-4(2016 10)

Endogenous airway mucins carry glycans that bind Siglec-F and induce eosinophil apoptosis.
Kiwamoto T, Katoh T, Evans CM, Janssen WJ, Brummet ME, Hudson SA, Zhu Z, Tiemeyer M, Bochner BS
The Journal of allergy and clinical immunology135(5)1329-1340.e9(2015 May)

Siglec-F-dependent negative regulation of allergen-induced eosinophilia depends critically on the experimental model.
McMillan SJ, Richards HE, Crocker PR
Immunology letters160(1)11-16(2014 Jul)

Galactose 6-O-sulfotransferases are not required for the generation of Siglec-F ligands in leukocytes or lung tissue.
Patnode ML, Cheng CW, Chou CC, Singer MS, Elin MS, Uchimura K, Crocker PR, Khoo KH, Rosen SD
The Journal of biological chemistry288(37)26533-45(2013 Sep)

Mechanisms of Siglec-F-induced eosinophil apoptosis: a role for caspases but not for SHP-1, Src kinases, NADPH oxidase or reactive oxygen.
Mao H, Kano G, Hudson SA, Brummet M, Zimmermann N, Zhu Z, Bochner BS
PloS one8(6)e68143(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Immunohistochemical analysis of the Peyer's patches (PPs) tissue sections shows that Siglec-F is expressed on the surface of the Intestinal microfold (M) cell membrane exposed to the intestinal lumen.
Title: Siglec-F is a novel intestinal M cell marker.

These data identify a previously unrecognized endogenous anti-inflammatory property of airway mucins by which their glycans can control lung eosinophilia through engagement of Siglec-F.
Title: Endogenous airway mucins carry glycans that bind Siglec-F and induce eosinophil apoptosis.

depending on the nature of the allergy model used, the negative regulatory role of siglec-F on eosinophilia may be overridden.
Title: Siglec-F-dependent negative regulation of allergen-induced eosinophilia depends critically on the experimental model.

neither KSGal6ST nor C6ST-1 is required for Siglec-F ligand synthesis in leukocytes or lung tissue
Title: Galactose 6-O-sulfotransferases are not required for the generation of Siglec-F ligands in leukocytes or lung tissue.

Engagement of Siglec-F induced mouse eosinophil apoptosis that was modest in magnitude and dependent on caspase activity.
Title: Mechanisms of Siglec-F-induced eosinophil apoptosis: a role for caspases but not for SHP-1, Src kinases, NADPH oxidase or reactive oxygen.

The following Siglecf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Siglecf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19380	NM_145581.2 Latest version!	Mus musculus sialic acid binding Ig-like lectin F (Siglecf), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu19534	NM_001271019.1 Latest version!	Mus musculus sialic acid binding Ig-like lectin F (Siglecf), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu38792	XM_006540816.1 Latest version!	Mus musculus sialic acid binding Ig-like lectin F (Siglecf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Siglecf ( NM_145581.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663556.1 Mus musculus sialic acid binding Ig-like lectin F (Siglecf), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19380
Clone ID Related Accession (Same CDS sequence)	NM_145581.2
Accession Version	NM_145581.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	sialic acid-binding Ig-like lectin 5 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB842394.1 and AF293371.1. On Aug 24, 2012 this sequence version replaced NM_145581.1. Transcript Variant: This variant (1) is the longer transcript and encodes a longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF293371.1, AK155469.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END##

ATGCGGTGGG CATGGCTGCT GCCCCTGCTG TGGGCAGGGT GCCTGGCTAC GGACGGTTAT 
TCGCTCTCAG TGACTGGTTC AGTGACAGTG CAGGAGGGCC TGTGCGTCTT TGTAGCCTGC 
CAAGTCCAGT ACCCCAATTC CAAAGGTCCT GTCTTTGGCT ACTGGTTCCG GGAGGGGGCC 
AATATATTCA GCGGCTCTCC AGTGGCTACA AATGACCCAC AGCGATCAGT ACTGAAGGAA 
GCTCAAGGTC GATTCTATCT CATGGGGAAG GAAAATAGTC ATAATTGCTC CCTGGACATC 
AGAGATGCAC AGAAAATCGA CACAGGGACG TACTTCTTCA GATTGGATGG TTCTGTGAAA 
TACAGTTTTC AGAAGTCAAT GCTCTCTGTG CTTGTGATAG CTCTGACTGA GGTCCCTAAC 
ATCCAAGTCA CATCTACCCT GGTGTCTGGC AATTCTACCA AACTGCTCTG TTCTGTGCCC 
TGGGCCTGTG AGCAGGGGAC ACCCCCCATC TTTTCCTGGA TGTCATCTGC CCTCACTTCC 
CTGGGCCACA GGACCACCCT CTCCTCAGAG CTGAACCTCA CACCCAGGCC TCAGGACAAT 
GGCACCAACC TCACCTGCCA GGTGAACTTA CCTGGCACTG GTGTTACTGT GGAGAGGACT 
CAACAGCTCA GTGTCATCTA TGCTCCACAG AAGATGACCA TCAGGGTGTC CTGGGGAGAT 
GACACAGGAA CTAAAGTCCT GCAGAGTGGA GCATCTCTGC AGATCCAAGA GGGTGAGTCC 
CTGAGCCTGG TCTGTATGGC TGACAGCAAT CCCCCTGCCG TGCTGAGTTG GGAGCGCCCG 
ACCCAAAAGC CCTTCCAGCT CTCTACCCCC GCGGAACTGC AACTGCCACG GGCGGAGTTG 
GAGGACCAGG GAAAATACAT CTGCCAAGCT CAGAACAGTC AGGGTGCTCA GACAGCCTCT 
GTGAGCCTCA GCATAAGGAG CCTTCTACAG CTGCTGGGAC CCTCCTGCTC CTTCGAGGGT 
CAAGGTCTGC ACTGCAGCTG CTCCTCCAGA GCCTGGCCTG CCCCCTCCCT GCGCTGGCGG 
CTGGGGGAGG GGGTGCTGGA GGGGAACAGC AGCAATGGCT CCTTCACGGT TAAGTCCAGC 
TCCGCAGGAC AGTGGGCCAA CAGCTCCCTG ATCCTCAGCA TGGAATTCAG CTCTAACCAC 
AGACTCAGCT GTGAAGCTTG GAGTGACAAT AGAGTTCAGA GAGCCACTAT CTTGCTGGTG 
TCAGGCCCAA AGGTCTCACA GGCAGGCAAA TCAGAAACCA GTAGAGGAAC GGTCCTGGGG 
GCCATCTGGG GAGCTGGTCT TATGGCCTTG CTTGCTGTCT GCCTCTGCCT CATCTTTTTC 
ACAGTGAAGG TCCTCAGGAA GAAATCAGCC CTGAAAGTAG CAGCTACGAA AGGCAACCAT 
CTTGCCAAGA ACCCTGCCTC CACCATAAAT AGTGCAAGCA TAACTTCATC CAACATTGCG 
TTGGGATACC CAATCCAGGG TCATCTGAAT GAACCAGGGT CACAGACCCA GAAAGAACAG 
CCACCCCTTG CCACAGTCCC AGACACCCAA AAGGATGAGC CTGAACTCCA CTATGCCTCC 
CTCTCCTTCC AAGGCCCGAT GCCCCCGAAG CCTCAGAACA CGGAGGCTAT GAAATCTGTA 
TACACAGAGA TCAAGATCCA CAAGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663556.1
CDS	105..1814
Translation	MRWAWLLPLLWAGCLATDGYSLSVTGSVTVQEGLCVFVACQVQYPNSKGPVFGYWFREGANIFSGSPVATNDPQRSVLKEAQGRFYLMGKENSHNCSLDIRDAQKIDTGTYFFRLDGSVKYSFQKSMLSVLVIALTEVPNIQVTSTLVSGNSTKLLCSVPWACEQGTPPIFSWMSSALTSLGHRTTLSSELNLTPRPQDNGTNLTCQVNLPGTGVTVERTQQLSVIYAPQKMTIRVSWGDDTGTKVLQSGASLQIQEGESLSLVCMADSNPPAVLSWERPTQKPFQLSTPAELQLPRAELEDQGKYICQAQNSQGAQTASVSLSIRSLLQLLGPSCSFEGQGLHCSCSSRAWPAPSLRWRLGEGVLEGNSSNGSFTVKSSSAGQWANSSLILSMEFSSNHRLSCEAWSDNRVQRATILLVSGPKVSQAGKSETSRGTVLGAIWGAGLMALLAVCLCLIFFTVKVLRKKSALKVAATKGNHLAKNPASTINSASITSSNIALGYPIQGHLNEPGSQTQKEQPPLATVPDTQKDEPELHYASLSFQGPMPPKPQNTEAMKSVYTEIKIHKC

Target ORF information:

RefSeq Version	NM_145581.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sialic acid binding Ig-like lectin F (Siglecf), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145581.2

ORF Insert Sequence:

ATGCGGTGGG CATGGCTGCT GCCCCTGCTG TGGGCAGGGT GCCTGGCTAC GGACGGTTAT 
TCGCTCTCAG TGACTGGTTC AGTGACAGTG CAGGAGGGCC TGTGCGTCTT TGTAGCCTGC 
CAAGTCCAGT ACCCCAATTC CAAAGGTCCT GTCTTTGGCT ACTGGTTCCG GGAGGGGGCC 
AATATATTCA GCGGCTCTCC AGTGGCTACA AATGACCCAC AGCGATCAGT ACTGAAGGAA 
GCTCAAGGTC GATTCTATCT CATGGGGAAG GAAAATAGTC ATAATTGCTC CCTGGACATC 
AGAGATGCAC AGAAAATCGA CACAGGGACG TACTTCTTCA GATTGGATGG TTCTGTGAAA 
TACAGTTTTC AGAAGTCAAT GCTCTCTGTG CTTGTGATAG CTCTGACTGA GGTCCCTAAC 
ATCCAAGTCA CATCTACCCT GGTGTCTGGC AATTCTACCA AACTGCTCTG TTCTGTGCCC 
TGGGCCTGTG AGCAGGGGAC ACCCCCCATC TTTTCCTGGA TGTCATCTGC CCTCACTTCC 
CTGGGCCACA GGACCACCCT CTCCTCAGAG CTGAACCTCA CACCCAGGCC TCAGGACAAT 
GGCACCAACC TCACCTGCCA GGTGAACTTA CCTGGCACTG GTGTTACTGT GGAGAGGACT 
CAACAGCTCA GTGTCATCTA TGCTCCACAG AAGATGACCA TCAGGGTGTC CTGGGGAGAT 
GACACAGGAA CTAAAGTCCT GCAGAGTGGA GCATCTCTGC AGATCCAAGA GGGTGAGTCC 
CTGAGCCTGG TCTGTATGGC TGACAGCAAT CCCCCTGCCG TGCTGAGTTG GGAGCGCCCG 
ACCCAAAAGC CCTTCCAGCT CTCTACCCCC GCGGAACTGC AACTGCCACG GGCGGAGTTG 
GAGGACCAGG GAAAATACAT CTGCCAAGCT CAGAACAGTC AGGGTGCTCA GACAGCCTCT 
GTGAGCCTCA GCATAAGGAG CCTTCTACAG CTGCTGGGAC CCTCCTGCTC CTTCGAGGGT 
CAAGGTCTGC ACTGCAGCTG CTCCTCCAGA GCCTGGCCTG CCCCCTCCCT GCGCTGGCGG 
CTGGGGGAGG GGGTGCTGGA GGGGAACAGC AGCAATGGCT CCTTCACGGT TAAGTCCAGC 
TCCGCAGGAC AGTGGGCCAA CAGCTCCCTG ATCCTCAGCA TGGAATTCAG CTCTAACCAC 
AGACTCAGCT GTGAAGCTTG GAGTGACAAT AGAGTTCAGA GAGCCACTAT CTTGCTGGTG 
TCAGGCCCAA AGGTCTCACA GGCAGGCAAA TCAGAAACCA GTAGAGGAAC GGTCCTGGGG 
GCCATCTGGG GAGCTGGTCT TATGGCCTTG CTTGCTGTCT GCCTCTGCCT CATCTTTTTC 
ACAGTGAAGG TCCTCAGGAA GAAATCAGCC CTGAAAGTAG CAGCTACGAA AGGCAACCAT 
CTTGCCAAGA ACCCTGCCTC CACCATAAAT AGTGCAAGCA TAACTTCATC CAACATTGCG 
TTGGGATACC CAATCCAGGG TCATCTGAAT GAACCAGGGT CACAGACCCA GAAAGAACAG 
CCACCCCTTG CCACAGTCCC AGACACCCAA AAGGATGAGC CTGAACTCCA CTATGCCTCC 
CTCTCCTTCC AAGGCCCGAT GCCCCCGAAG CCTCAGAACA CGGAGGCTAT GAAATCTGTA 
TACACAGAGA TCAAGATCCA CAAGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Siglecf ( NM_001271019.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001257948.1 Mus musculus sialic acid binding Ig-like lectin F (Siglecf), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19534
Clone ID Related Accession (Same CDS sequence)	NM_001271019.1
Accession Version	NM_001271019.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	sialic acid-binding Ig-like lectin 5 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB842394.1, BC145038.1 and AF293371.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145038.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGCGGTGGG CATGGCTGCT GCCCCTGCTG TGGGCAGGGT GCCTGGCTAC GGACGGTTAT 
TCGCTCTCAG TGACTGGTTC AGTGACAGTG CAGGAGGGCC TGTGCGTCTT TGTAGCCTGC 
CAAGTCCAGT ACCCCAATTC CAAAGGTCCT GTCTTTGGCT ACTGGTTCCG GGAGGGGGCC 
AATATATTCA GCGGCTCTCC AGTGGCTACA AATGACCCAC AGCGATCAGT ACTGAAGGAA 
GCTCAAGGTC GATTCTATCT CATGGGGAAG GAAAATAGTC ATAATTGCTC CCTGGACATC 
AGAGATGCAC AGAAAATCGA CACAGGGACG TACTTCTTCA GATTGGATGG TTCTGTGAAA 
TACAGTTTTC AGAAGTCAAT GCTCTCTGTG CTTGTGATAG CTCTGACTGA GGTCCCTAAC 
ATCCAAGTCA CATCTACCCT GGTGTCTGGC AATTCTACCA AACTGCTCTG TTCTGTGCCC 
TGGGCCTGTG AGCAGGGGAC ACCCCCCATC TTTTCCTGGA TGTCATCTGC CCTCACTTCC 
CTGGGCCACA GGACCACCCT CTCCTCAGAG CTGAACCTCA CACCCAGGCC TCAGGACAAT 
GGCACCAACC TCACCTGCCA GGTGAACTTA CCTGGCACTG GTGTTACTGT GGAGAGGACT 
CAACAGCTCA GTGTCATCTA TGCTCCACAG AAGATGACCA TCAGGGTGTC CTGGGGAGAT 
GACACAGGAA CTAAAGTCCT GCAGAGTGGA GCATCTCTGC AGATCCAAGA GGGTGAGTCC 
CTGAGCCTGG TCTGTATGGC TGACAGCAAT CCCCCTGCCG TGCTGAGTTG GGAGCGCCCG 
ACCCAAAAGC CCTTCCAGCT CTCTACCCCC GCGGAACTGC AACTGCCACG GGCGGAGTTG 
GAGGACCAGG GAAAATACAT CTGCCAAGCT CAGAACAGTC AGGGTGCTCA GACAGCCTCT 
GTGAGCCTCA GCATAAGGAG CCTTCTACAG CTGCTGGGAC CCTCCTGCTC CTTCGAGGGT 
CAAGGTCTGC ACTGCAGCTG CTCCTCCAGA GCCTGGCCTG CCCCCTCCCT GCGCTGGCGG 
CTGGGGGAGG GGGTGCTGGA GGGGAACAGC AGCAATGGCT CCTTCACGGT TAAGTCCAGC 
TCCGCAGGAC AGTGGGCCAA CAGCTCCCTG ATCCTCAGCA TGGAATTCAG CTCTAACCAC 
AGACTCAGCT GTGAAGCTTG GAGTGACAAT AGAGTTCAGA GAGCCACTAT CTTGCTGGTG 
TCAGGCCCAA AGGTCTCACA GGTGAAGGTC CTCAGGAAGA AATCAGCCCT GAAAGTAGCA 
GCTACGAAAG GCAACCATCT TGCCAAGAAC CCTGCCTCCA CCATAAATAG TGCAAGCATA 
ACTTCATCCA ACATTGCGTT GGGATACCCA ATCCAGGGTC ATCTGAATGA ACCAGGGTCA 
CAGACCCAGA AAGAACAGCC ACCCCTTGCC ACAGTCCCAG ACACCCAAAA GGATGAGCCT 
GAACTCCACT ATGCCTCCCT CTCCTTCCAA GGCCCGATGC CCCCGAAGCC TCAGAACACG 
GAGGCTATGA AATCTGTATA CACAGAGATC AAGATCCACA AGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257948.1
CDS	105..1712
Translation	MRWAWLLPLLWAGCLATDGYSLSVTGSVTVQEGLCVFVACQVQYPNSKGPVFGYWFREGANIFSGSPVATNDPQRSVLKEAQGRFYLMGKENSHNCSLDIRDAQKIDTGTYFFRLDGSVKYSFQKSMLSVLVIALTEVPNIQVTSTLVSGNSTKLLCSVPWACEQGTPPIFSWMSSALTSLGHRTTLSSELNLTPRPQDNGTNLTCQVNLPGTGVTVERTQQLSVIYAPQKMTIRVSWGDDTGTKVLQSGASLQIQEGESLSLVCMADSNPPAVLSWERPTQKPFQLSTPAELQLPRAELEDQGKYICQAQNSQGAQTASVSLSIRSLLQLLGPSCSFEGQGLHCSCSSRAWPAPSLRWRLGEGVLEGNSSNGSFTVKSSSAGQWANSSLILSMEFSSNHRLSCEAWSDNRVQRATILLVSGPKVSQVKVLRKKSALKVAATKGNHLAKNPASTINSASITSSNIALGYPIQGHLNEPGSQTQKEQPPLATVPDTQKDEPELHYASLSFQGPMPPKPQNTEAMKSVYTEIKIHKC

Target ORF information:

RefSeq Version	NM_001271019.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sialic acid binding Ig-like lectin F (Siglecf), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271019.1

ORF Insert Sequence:

ATGCGGTGGG CATGGCTGCT GCCCCTGCTG TGGGCAGGGT GCCTGGCTAC GGACGGTTAT 
TCGCTCTCAG TGACTGGTTC AGTGACAGTG CAGGAGGGCC TGTGCGTCTT TGTAGCCTGC 
CAAGTCCAGT ACCCCAATTC CAAAGGTCCT GTCTTTGGCT ACTGGTTCCG GGAGGGGGCC 
AATATATTCA GCGGCTCTCC AGTGGCTACA AATGACCCAC AGCGATCAGT ACTGAAGGAA 
GCTCAAGGTC GATTCTATCT CATGGGGAAG GAAAATAGTC ATAATTGCTC CCTGGACATC 
AGAGATGCAC AGAAAATCGA CACAGGGACG TACTTCTTCA GATTGGATGG TTCTGTGAAA 
TACAGTTTTC AGAAGTCAAT GCTCTCTGTG CTTGTGATAG CTCTGACTGA GGTCCCTAAC 
ATCCAAGTCA CATCTACCCT GGTGTCTGGC AATTCTACCA AACTGCTCTG TTCTGTGCCC 
TGGGCCTGTG AGCAGGGGAC ACCCCCCATC TTTTCCTGGA TGTCATCTGC CCTCACTTCC 
CTGGGCCACA GGACCACCCT CTCCTCAGAG CTGAACCTCA CACCCAGGCC TCAGGACAAT 
GGCACCAACC TCACCTGCCA GGTGAACTTA CCTGGCACTG GTGTTACTGT GGAGAGGACT 
CAACAGCTCA GTGTCATCTA TGCTCCACAG AAGATGACCA TCAGGGTGTC CTGGGGAGAT 
GACACAGGAA CTAAAGTCCT GCAGAGTGGA GCATCTCTGC AGATCCAAGA GGGTGAGTCC 
CTGAGCCTGG TCTGTATGGC TGACAGCAAT CCCCCTGCCG TGCTGAGTTG GGAGCGCCCG 
ACCCAAAAGC CCTTCCAGCT CTCTACCCCC GCGGAACTGC AACTGCCACG GGCGGAGTTG 
GAGGACCAGG GAAAATACAT CTGCCAAGCT CAGAACAGTC AGGGTGCTCA GACAGCCTCT 
GTGAGCCTCA GCATAAGGAG CCTTCTACAG CTGCTGGGAC CCTCCTGCTC CTTCGAGGGT 
CAAGGTCTGC ACTGCAGCTG CTCCTCCAGA GCCTGGCCTG CCCCCTCCCT GCGCTGGCGG 
CTGGGGGAGG GGGTGCTGGA GGGGAACAGC AGCAATGGCT CCTTCACGGT TAAGTCCAGC 
TCCGCAGGAC AGTGGGCCAA CAGCTCCCTG ATCCTCAGCA TGGAATTCAG CTCTAACCAC 
AGACTCAGCT GTGAAGCTTG GAGTGACAAT AGAGTTCAGA GAGCCACTAT CTTGCTGGTG 
TCAGGCCCAA AGGTCTCACA GGTGAAGGTC CTCAGGAAGA AATCAGCCCT GAAAGTAGCA 
GCTACGAAAG GCAACCATCT TGCCAAGAAC CCTGCCTCCA CCATAAATAG TGCAAGCATA 
ACTTCATCCA ACATTGCGTT GGGATACCCA ATCCAGGGTC ATCTGAATGA ACCAGGGTCA 
CAGACCCAGA AAGAACAGCC ACCCCTTGCC ACAGTCCCAG ACACCCAAAA GGATGAGCCT 
GAACTCCACT ATGCCTCCCT CTCCTTCCAA GGCCCGATGC CCCCGAAGCC TCAGAACACG 
GAGGCTATGA AATCTGTATA CACAGAGATC AAGATCCACA AGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Siglecf ( XM_006540816.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540879.1 Mus musculus sialic acid binding Ig-like lectin F (Siglecf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu38792
Clone ID Related Accession (Same CDS sequence)	XM_006540816.1
Accession Version	XM_006540816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sialic acid-binding Ig-like lectin 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGTGGG CATGGCTGCT GCCCCTGCTG TGGGCAGGGT GCCTGGCTAC GGACGGTTAT 
TCGCTCTCAG TGACTGGTTC AGTGACAGTG CAGGAGGGCC TGTGCGTCTT TGTAGCCTGC 
CAAGTCCAGT ACCCCAATTC CAAAGGTCCT GTCTTTGGCT ACTGGTTCCG GGAGGGGGCC 
AATATATTCA GCGGCTCTCC AGTGGCTACA AATGACCCAC AGCGATCAGT ACTGAAGGAA 
GCTCAAGGTC GATTCTATCT CATGGGGAAG GAAAATAGTC ATAATTGCTC CCTGGACATC 
AGAGATGCAC AGAAAATCGA CACAGGGACG TACTTCTTCA GATTGGATGG TTCTGTGAAA 
TACAGTTTTC AGAAGTCAAT GCTCTCTGTG CTTGTGATAG CTCTGACTGA GGTCCCTAAC 
ATCCAAGTCA CATCTACCCT GGTGTCTGGC AATTCTACCA AACTGCTCTG TTCTGTGCCC 
TGGGCCTGTG AGCAGGGGAC ACCCCCCATC TTTTCCTGGA TGTCATCTGC CCTCACTTCC 
CTGGGCCACA GGACCACCCT CTCCTCAGAG CTGAACCTCA CACCCAGGCC TCAGGACAAT 
GGCACCAACC TCACCTGCCA GGTGAACTTA CCTGGCACTG GTGTTACTGT GGAGAGGACT 
CAACAGCTCA GTGTCATCTA TGCTCCACAG AAGATGACCA TCAGGGTGTC CTGGGGAGAT 
GACACAGGAA CTAAAGTCCT GCAGAGTGGA GCATCTCTGC AGATCCAAGA GGGTGAGTCC 
CTGAGCCTGG TCTGTATGGC TGACAGCAAT CCCCCTGCCG TGCTGAGTTG GGAGCGCCCG 
ACCCAAAAGC CCTTCCAGCT CTCTACCCCC GCGGAACTGC AACTGCCACG GGCGGAGTTG 
GAGGACCAGG GAAAATACAT CTGCCAAGCT CAGAACAGTC AGGGTGCTCA GACAGCCTCT 
GTGAGCCTCA GCATAAGGAG CCTTCTACAG CTGCTGGGAC CCTCCTGCTC CTTCGAGGGT 
CAAGGTCTGC ACTGCAGCTG CTCCTCCAGA GCCTGGCCTG CCCCCTCCCT GCGCTGGCGG 
CTGGGGGAGG GGGTGCTGGA GGGGAACAGC AGCAATGGCT CCTTCACGGT TAAGTCCAGC 
TCCGCAGGAC AGTGGGCCAA CAGCTCCCTG ATCCTCAGCA TGGAATTCAG CTCTAACCAC 
AGACTCAGCT GTGAAGCTTG GAGTGACAAT AGAGTTCAGA GAGCCACTAT CTTGCTGGTG 
TCAGGCCCAA AGGTCTCACA GGGCAAATCA GAAACCAGTA GAGGAACGGT CCTGGGGGCC 
ATCTGGGGAG CTGGTCTTAT GGCCTTGCTT GCTGTCTGCC TCTGCCTCAT CTTTTTCACA 
GTGAAGGTCC TCAGGAAGAA ATCAGCCCTG AAAGTAGCAG CTACGAAAGG CAACCATCTT 
GCCAAGAACC CTGCCTCCAC CATAAATAGT GCAAGCATAA CTTCATCCAA CATTGCGTTG 
GGATACCCAA TCCAGGGTCA TCTGAATGAA CCAGGGTCAC AGACCCAGAA AGAACAGCCA 
CCCCTTGCCA CAGTCCCAGA CACCCAAAAG GATGAGCCTG AACTCCACTA TGCCTCCCTC 
TCCTTCCAAG GCCCGATGCC CCCGAAGCCT CAGAACACGG AGGCTATGAA ATCTGTATAC 
ACAGAGATCA AGATCCACAA GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540879.1
CDS	135..1841
Translation	MRWAWLLPLLWAGCLATDGYSLSVTGSVTVQEGLCVFVACQVQYPNSKGPVFGYWFREGANIFSGSPVATNDPQRSVLKEAQGRFYLMGKENSHNCSLDIRDAQKIDTGTYFFRLDGSVKYSFQKSMLSVLVIALTEVPNIQVTSTLVSGNSTKLLCSVPWACEQGTPPIFSWMSSALTSLGHRTTLSSELNLTPRPQDNGTNLTCQVNLPGTGVTVERTQQLSVIYAPQKMTIRVSWGDDTGTKVLQSGASLQIQEGESLSLVCMADSNPPAVLSWERPTQKPFQLSTPAELQLPRAELEDQGKYICQAQNSQGAQTASVSLSIRSLLQLLGPSCSFEGQGLHCSCSSRAWPAPSLRWRLGEGVLEGNSSNGSFTVKSSSAGQWANSSLILSMEFSSNHRLSCEAWSDNRVQRATILLVSGPKVSQGKSETSRGTVLGAIWGAGLMALLAVCLCLIFFTVKVLRKKSALKVAATKGNHLAKNPASTINSASITSSNIALGYPIQGHLNEPGSQTQKEQPPLATVPDTQKDEPELHYASLSFQGPMPPKPQNTEAMKSVYTEIKIHKC

Target ORF information:

RefSeq Version	XM_006540816.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sialic acid binding Ig-like lectin F (Siglecf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540816.1

ORF Insert Sequence:

ATGCGGTGGG CATGGCTGCT GCCCCTGCTG TGGGCAGGGT GCCTGGCTAC GGACGGTTAT 
TCGCTCTCAG TGACTGGTTC AGTGACAGTG CAGGAGGGCC TGTGCGTCTT TGTAGCCTGC 
CAAGTCCAGT ACCCCAATTC CAAAGGTCCT GTCTTTGGCT ACTGGTTCCG GGAGGGGGCC 
AATATATTCA GCGGCTCTCC AGTGGCTACA AATGACCCAC AGCGATCAGT ACTGAAGGAA 
GCTCAAGGTC GATTCTATCT CATGGGGAAG GAAAATAGTC ATAATTGCTC CCTGGACATC 
AGAGATGCAC AGAAAATCGA CACAGGGACG TACTTCTTCA GATTGGATGG TTCTGTGAAA 
TACAGTTTTC AGAAGTCAAT GCTCTCTGTG CTTGTGATAG CTCTGACTGA GGTCCCTAAC 
ATCCAAGTCA CATCTACCCT GGTGTCTGGC AATTCTACCA AACTGCTCTG TTCTGTGCCC 
TGGGCCTGTG AGCAGGGGAC ACCCCCCATC TTTTCCTGGA TGTCATCTGC CCTCACTTCC 
CTGGGCCACA GGACCACCCT CTCCTCAGAG CTGAACCTCA CACCCAGGCC TCAGGACAAT 
GGCACCAACC TCACCTGCCA GGTGAACTTA CCTGGCACTG GTGTTACTGT GGAGAGGACT 
CAACAGCTCA GTGTCATCTA TGCTCCACAG AAGATGACCA TCAGGGTGTC CTGGGGAGAT 
GACACAGGAA CTAAAGTCCT GCAGAGTGGA GCATCTCTGC AGATCCAAGA GGGTGAGTCC 
CTGAGCCTGG TCTGTATGGC TGACAGCAAT CCCCCTGCCG TGCTGAGTTG GGAGCGCCCG 
ACCCAAAAGC CCTTCCAGCT CTCTACCCCC GCGGAACTGC AACTGCCACG GGCGGAGTTG 
GAGGACCAGG GAAAATACAT CTGCCAAGCT CAGAACAGTC AGGGTGCTCA GACAGCCTCT 
GTGAGCCTCA GCATAAGGAG CCTTCTACAG CTGCTGGGAC CCTCCTGCTC CTTCGAGGGT 
CAAGGTCTGC ACTGCAGCTG CTCCTCCAGA GCCTGGCCTG CCCCCTCCCT GCGCTGGCGG 
CTGGGGGAGG GGGTGCTGGA GGGGAACAGC AGCAATGGCT CCTTCACGGT TAAGTCCAGC 
TCCGCAGGAC AGTGGGCCAA CAGCTCCCTG ATCCTCAGCA TGGAATTCAG CTCTAACCAC 
AGACTCAGCT GTGAAGCTTG GAGTGACAAT AGAGTTCAGA GAGCCACTAT CTTGCTGGTG 
TCAGGCCCAA AGGTCTCACA GGGCAAATCA GAAACCAGTA GAGGAACGGT CCTGGGGGCC 
ATCTGGGGAG CTGGTCTTAT GGCCTTGCTT GCTGTCTGCC TCTGCCTCAT CTTTTTCACA 
GTGAAGGTCC TCAGGAAGAA ATCAGCCCTG AAAGTAGCAG CTACGAAAGG CAACCATCTT 
GCCAAGAACC CTGCCTCCAC CATAAATAGT GCAAGCATAA CTTCATCCAA CATTGCGTTG 
GGATACCCAA TCCAGGGTCA TCTGAATGAA CCAGGGTCAC AGACCCAGAA AGAACAGCCA 
CCCCTTGCCA CAGTCCCAGA CACCCAAAAG GATGAGCCTG AACTCCACTA TGCCTCCCTC 
TCCTTCCAAG GCCCGATGCC CCCGAAGCCT CAGAACACGG AGGCTATGAA ATCTGTATAC 
ACAGAGATCA AGATCCACAA GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Siglec-F is a novel intestinal M cell marker.
Biochemical and biophysical research communications479(1)1-4(2016 10)
Gicheva N,Macauley MS,Arlian BM,Paulson JC,Kawasaki N

Endogenous airway mucins carry glycans that bind Siglec-F and induce eosinophil apoptosis.
The Journal of allergy and clinical immunology135(5)1329-1340.e9(2015 May)
Kiwamoto T,Katoh T,Evans CM,Janssen WJ,Brummet ME,Hudson SA,Zhu Z,Tiemeyer M,Bochner BS

Siglec-F-dependent negative regulation of allergen-induced eosinophilia depends critically on the experimental model.
Immunology letters160(1)11-16(2014 Jul)
McMillan SJ,Richards HE,Crocker PR

Galactose 6-O-sulfotransferases are not required for the generation of Siglec-F ligands in leukocytes or lung tissue.
The Journal of biological chemistry288(37)26533-45(2013 Sep)
Patnode ML,Cheng CW,Chou CC,Singer MS,Elin MS,Uchimura K,Crocker PR,Khoo KH,Rosen SD

Mechanisms of Siglec-F-induced eosinophil apoptosis: a role for caspases but not for SHP-1, Src kinases, NADPH oxidase or reactive oxygen.
PloS one8(6)e68143(2013)
Mao H,Kano G,Hudson SA,Brummet M,Zimmermann N,Zhu Z,Bochner BS