Ppp4r2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp4r2
Entrez Gene ID	232314
Full Name	protein phosphatase 4, regulatory subunit 2
Synonyms	BE691708,C230060M08Rik
General protein information	Preferred Names protein phosphatase 4, regulatory subunit 2 Names serine/threonine-protein phosphatase 4 regulatory subunit 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 D3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006506008.4	XP_006506071.1	serine/threonine-protein phosphatase 4 regulatory subunit 2 isoform X1
NM_182939.4	NP_891984.1	serine/threonine-protein phosphatase 4 regulatory subunit 2
XM_006506008.3	XP_006506071.1	serine/threonine-protein phosphatase 4 regulatory subunit 2 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ppp4r2	NP_001100083.2
Gallus gallus (chicken)	PPP4R2	NP_001006142.1
Homo sapiens (human)	PPP4R2	NP_777567.1
Bos taurus (cattle)	PPP4R2	XP_002697003.2
	PPP4R2	XP_002802782.1
Mus musculus (house mouse)	Ppp4r2	NP_891984.1
Xenopus tropicalis (tropical clawed frog)	ppp4r2	NP_001011151.1
Pan troglodytes (chimpanzee)	PPP4R2	XP_003309936.1
Canis lupus familiaris (dog)	PPP4R2	XP_005632308.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Haploinsufficiency of SAMD9L, an endosome fusion facilitator, causes myeloid malignancies in mice mimicking human diseases with monosomy 7.
Nagamachi A, Matsui H, Asou H, Ozaki Y, Aki D, Kanai A, Takubo K, Suda T, Nakamura T, Wolff L, Honda H, Inaba T
Cancer cell24(3)305-17(2013 Sep)

Suppressor of MEK null (SMEK)/protein phosphatase 4 catalytic subunit (PP4C) is a key regulator of hepatic gluconeogenesis.
Yoon YS, Lee MW, Ryu D, Kim JH, Ma H, Seo WY, Kim YN, Kim SS, Lee CH, Hunter T, Choi CS, Montminy MR, Koo SH
Proceedings of the National Academy of Sciences of the United States of America107(41)17704-9(2010 Oct)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ppp4r2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp4r2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19980	NM_182939.4 Latest version!	Mus musculus protein phosphatase 4, regulatory subunit 2 (Ppp4r2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
OMu37194	XM_006506008.4 Latest version!	Mus musculus protein phosphatase 4, regulatory subunit 2 (Ppp4r2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu37194	XM_006506008.3	Mus musculus protein phosphatase 4, regulatory subunit 2 (Ppp4r2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp4r2 ( NM_182939.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_891984.1 Mus musculus protein phosphatase 4, regulatory subunit 2 (Ppp4r2), mRNA.

Price & Availability↑

CloneID	OMu19980
Clone ID Related Accession (Same CDS sequence)	NM_182939.4
Accession Version	NM_182939.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein phosphatase 4 regulatory subunit 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC155648.14. On Oct 19, 2007 this sequence version replaced NM_182939.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK082539.1, BC098464.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGACGTCG AGAGGCTGCA GGAGGCGCTG AAAGATTTTG AGAAGAGAGG GAAAAAGGAA 
GTTTGTCCTG TACTGGATCA GTTTCTCTGT CATGTAGCTA AAACTGGAGA AACAATGATT 
CAGTGGTCCC AATTTAAAGG CTATTTCATT TTCAAACTGG AGAAAGTGAT GGATGATTTC 
AGAACTTCAG CTCCTGAACC AAGAGGTCCT CCCAATCCTA ATGTTGAATA TATTCCCTTT 
GATGAAATGA AGGAAAGAAT ACTGAAAATT GTCACTGGAT TTAATGGTAT CCCTTTTACT 
ATTCAGCGGC TATGTGAATT GCTAACGGAT CCGAGAAGAA ACTATACAGG AACAGACAAG 
TTTCTCAGAG GAGTAGAGAA GAATGTGATG GTTGTTAGCT GCGTTTGTCC ATCCTCAGAG 
AAGAACAATT CTAATAGCTT AAATAGAATG AATGGTGTTA TGTTTCCTGG AAACTCACCA 
AACTATACTG ACAGGTCTAA TATAAACGGG CCTGGAACAC CTAGGCCACT TAATCGACCA 
AAGCTTTCTT TGTCAGCCCC CTTGACAACA AATGGTTTGC CTGAGAGCAC AGATAGCAAA 
GATTCTGAGC TGCAGCTAAG TGAAGAGAAA GGCCACAGTG ATTCTTCAGC CTCTGAATCA 
GAAGTTTCCT TACTGAGCCC TGTTAAAAAT AAACATCCAG ATGAAGATGC TGTGGAGTCT 
GAGGAACATG AGGTGAAAAG ACTGAAGTTT GACAAAGAAG GTGACGTCAG AGAGACAGCT 
AGCCAAACGG TGTCTGGTGA AGTCTCTTCA GTTAGAGCAG AGGAAACGGA AACAGCAGCT 
CCCCCTCCTG ACAAGGACAG AGAAAGTCGA ACCAGGCAGC ACTGTACAGA AGAGGAGGAG 
GAGGAGGAGG AAGAAGAGGA AGAGGAGGAG GAAGAATCGT TTATGACACC AAGAGAAATG 
GTCCCAGAAA GAAAAAATCA AGAAAAGGAA TCTGATGACG CCTTAACTGT GAATGAAGAG 
ACTTCAGAGG AGAGCCATCA GATGGAAGGC TCTGGTGTGT CTCCAGCTCA GACAGACTCC 
ACTTCAGAAA GGAGTGACAG TGCAGGGGCC TCAAGGAGTG GCTCTGACTG CCTGGAGACA 
CAGGAGTCAG GAGGGCCCCC TTCCAGTAAG ACTGGAGAGA GTGTGTCAGT GCCGTCGTCC 
ATGGAGAGTG AGGAAGCCGC AGAAGTCACA GATGACCCAA TGGAGCAAGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_891984.1
CDS	247..1500
Translation	MDVERLQEALKDFEKRGKKEVCPVLDQFLCHVAKTGETMIQWSQFKGYFIFKLEKVMDDFRTSAPEPRGPPNPNVEYIPFDEMKERILKIVTGFNGIPFTIQRLCELLTDPRRNYTGTDKFLRGVEKNVMVVSCVCPSSEKNNSNSLNRMNGVMFPGNSPNYTDRSNINGPGTPRPLNRPKLSLSAPLTTNGLPESTDSKDSELQLSEEKGHSDSSASESEVSLLSPVKNKHPDEDAVESEEHEVKRLKFDKEGDVRETASQTVSGEVSSVRAEETETAAPPPDKDRESRTRQHCTEEEEEEEEEEEEEEEESFMTPREMVPERKNQEKESDDALTVNEETSEESHQMEGSGVSPAQTDSTSERSDSAGASRSGSDCLETQESGGPPSSKTGESVSVPSSMESEEAAEVTDDPMEQD

Target ORF information:

RefSeq Version	NM_182939.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 4, regulatory subunit 2 (Ppp4r2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182939.4

ORF Insert Sequence:

ATGGACGTCG AGAGGCTGCA GGAGGCGCTG AAAGATTTTG AGAAGAGAGG GAAAAAGGAA 
GTTTGTCCTG TACTGGATCA GTTTCTCTGT CATGTAGCTA AAACTGGAGA AACAATGATT 
CAGTGGTCCC AATTTAAAGG CTATTTCATT TTCAAACTGG AGAAAGTGAT GGATGATTTC 
AGAACTTCAG CTCCTGAACC AAGAGGTCCT CCCAATCCTA ATGTTGAATA TATTCCCTTT 
GATGAAATGA AGGAAAGAAT ACTGAAAATT GTCACTGGAT TTAATGGTAT CCCTTTTACT 
ATTCAGCGGC TATGTGAATT GCTAACGGAT CCGAGAAGAA ACTATACAGG AACAGACAAG 
TTTCTCAGAG GAGTAGAGAA GAATGTGATG GTTGTTAGCT GCGTTTGTCC ATCCTCAGAG 
AAGAACAATT CTAATAGCTT AAATAGAATG AATGGTGTTA TGTTTCCTGG AAACTCACCA 
AACTATACTG ACAGGTCTAA TATAAACGGG CCTGGAACAC CTAGGCCACT TAATCGACCA 
AAGCTTTCTT TGTCAGCCCC CTTGACAACA AATGGTTTGC CTGAGAGCAC AGATAGCAAA 
GATTCTGAGC TGCAGCTAAG TGAAGAGAAA GGCCACAGTG ATTCTTCAGC CTCTGAATCA 
GAAGTTTCCT TACTGAGCCC TGTTAAAAAT AAACATCCAG ATGAAGATGC TGTGGAGTCT 
GAGGAACATG AGGTGAAAAG ACTGAAGTTT GACAAAGAAG GTGACGTCAG AGAGACAGCT 
AGCCAAACGG TGTCTGGTGA AGTCTCTTCA GTTAGAGCAG AGGAAACGGA AACAGCAGCT 
CCCCCTCCTG ACAAGGACAG AGAAAGTCGA ACCAGGCAGC ACTGTACAGA AGAGGAGGAG 
GAGGAGGAGG AAGAAGAGGA AGAGGAGGAG GAAGAATCGT TTATGACACC AAGAGAAATG 
GTCCCAGAAA GAAAAAATCA AGAAAAGGAA TCTGATGACG CCTTAACTGT GAATGAAGAG 
ACTTCAGAGG AGAGCCATCA GATGGAAGGC TCTGGTGTGT CTCCAGCTCA GACAGACTCC 
ACTTCAGAAA GGAGTGACAG TGCAGGGGCC TCAAGGAGTG GCTCTGACTG CCTGGAGACA 
CAGGAGTCAG GAGGGCCCCC TTCCAGTAAG ACTGGAGAGA GTGTGTCAGT GCCGTCGTCC 
ATGGAGAGTG AGGAAGCCGC AGAAGTCACA GATGACCCAA TGGAGCAAGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp4r2 ( XM_006506008.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506071.1 Mus musculus protein phosphatase 4, regulatory subunit 2 (Ppp4r2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu37194
Clone ID Related Accession (Same CDS sequence)	XM_006506008.4 , XM_006506008.3
Accession Version	XM_006506008.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein phosphatase 4 regulatory subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506008.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTCG AGAGGCTGCA GGAGGCGCTG AAAGATTTTG AGAAGAGAGG GAAAAAGGAA 
GTTTGTCCTG TACTGGATCA GTTTCTCTGT CATGTAGCTA AAACTGGAGA AACAATGATT 
CAGTGGTCCC AATTTAAAGG CTATTTCATT TTCAAACTGG AGAAAGTGAT GGATGATTTC 
AGAACTTCAG CTCCTGAACC AAGAGGTCCT CCCAATCCTA ATGTTGAATA TATTCCCTTT 
GATGAAATGA AGGAAAGAAT ACTGAAAATT GTCACTGGAT TTAATGGTAT CCCTTTTACT 
ATTCAGCGGC TATGTGAATT GCTAACGGAT CCGAGAAGAA ACTATACAGG AACAGACAAG 
TTTCTCAGAG GAGTAGAGAA GAATGTGATG GTTGTTAGCT GCGTTTGTCC ATCCTCAGAG 
AAGAACAATT CTAATAGCTT AAATAGAATG AATGGTGTTA TGTTTCCTGG AAACTCACCA 
AACTATACTG ACAGTGATTC TTCAGCCTCT GAATCAGAAG TTTCCTTACT GAGCCCTGTT 
AAAAATAAAC ATCCAGATGA AGATGCTGTG GAGTCTGAGG AACATGAGGT GAAAAGACTG 
AAGTTTGACA AAGAAGGTGA CGTCAGAGAG ACAGCTAGCC AAACGGTGTC TGGTGAAGTC 
TCTTCAGTTA GAGCAGAGGA AACGGAAACA GCAGCTCCCC CTCCTGACAA GGACAGAGAA 
AGTCGAACCA GGCAGCACTG TACAGAAGAG GAGGAGGAGG AGGAGGAAGA AGAGGAAGAG 
GAGGAGGAAG AATCGTTTAT GACACCAAGA GAAATGGTCC CAGAAAGAAA AAATCAAGAA 
AAGGAATCTG ATGACGCCTT AACTGTGAAT GAAGAGACTT CAGAGGAGAG CCATCAGATG 
GAAGGCTCTG GTGTGTCTCC AGCTCAGACA GACTCCACTT CAGAAAGGAG TGACAGTGCA 
GGGGCCTCAA GGAGTGGCTC TGACTGCCTG GAGACACAGG AGTCAGGAGG GCCCCCTTCC 
AGTAAGACTG GAGAGAGTGT GTCAGTGCCG TCGTCCATGG AGAGTGAGGA AGCCGCAGAA 
GTCACAGATG ACCCAATGGA GCAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506071.1
CDS	265..1374
Translation	MDVERLQEALKDFEKRGKKEVCPVLDQFLCHVAKTGETMIQWSQFKGYFIFKLEKVMDDFRTSAPEPRGPPNPNVEYIPFDEMKERILKIVTGFNGIPFTIQRLCELLTDPRRNYTGTDKFLRGVEKNVMVVSCVCPSSEKNNSNSLNRMNGVMFPGNSPNYTDSDSSASESEVSLLSPVKNKHPDEDAVESEEHEVKRLKFDKEGDVRETASQTVSGEVSSVRAEETETAAPPPDKDRESRTRQHCTEEEEEEEEEEEEEEEESFMTPREMVPERKNQEKESDDALTVNEETSEESHQMEGSGVSPAQTDSTSERSDSAGASRSGSDCLETQESGGPPSSKTGESVSVPSSMESEEAAEVTDDPMEQD

Target ORF information:

RefSeq Version	XM_006506008.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 4, regulatory subunit 2 (Ppp4r2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506008.4

ORF Insert Sequence:

ATGGACGTCG AGAGGCTGCA GGAGGCGCTG AAAGATTTTG AGAAGAGAGG GAAAAAGGAA 
GTTTGTCCTG TACTGGATCA GTTTCTCTGT CATGTAGCTA AAACTGGAGA AACAATGATT 
CAGTGGTCCC AATTTAAAGG CTATTTCATT TTCAAACTGG AGAAAGTGAT GGATGATTTC 
AGAACTTCAG CTCCTGAACC AAGAGGTCCT CCCAATCCTA ATGTTGAATA TATTCCCTTT 
GATGAAATGA AGGAAAGAAT ACTGAAAATT GTCACTGGAT TTAATGGTAT CCCTTTTACT 
ATTCAGCGGC TATGTGAATT GCTAACGGAT CCGAGAAGAA ACTATACAGG AACAGACAAG 
TTTCTCAGAG GAGTAGAGAA GAATGTGATG GTTGTTAGCT GCGTTTGTCC ATCCTCAGAG 
AAGAACAATT CTAATAGCTT AAATAGAATG AATGGTGTTA TGTTTCCTGG AAACTCACCA 
AACTATACTG ACAGTGATTC TTCAGCCTCT GAATCAGAAG TTTCCTTACT GAGCCCTGTT 
AAAAATAAAC ATCCAGATGA AGATGCTGTG GAGTCTGAGG AACATGAGGT GAAAAGACTG 
AAGTTTGACA AAGAAGGTGA CGTCAGAGAG ACAGCTAGCC AAACGGTGTC TGGTGAAGTC 
TCTTCAGTTA GAGCAGAGGA AACGGAAACA GCAGCTCCCC CTCCTGACAA GGACAGAGAA 
AGTCGAACCA GGCAGCACTG TACAGAAGAG GAGGAGGAGG AGGAGGAAGA AGAGGAAGAG 
GAGGAGGAAG AATCGTTTAT GACACCAAGA GAAATGGTCC CAGAAAGAAA AAATCAAGAA 
AAGGAATCTG ATGACGCCTT AACTGTGAAT GAAGAGACTT CAGAGGAGAG CCATCAGATG 
GAAGGCTCTG GTGTGTCTCC AGCTCAGACA GACTCCACTT CAGAAAGGAG TGACAGTGCA 
GGGGCCTCAA GGAGTGGCTC TGACTGCCTG GAGACACAGG AGTCAGGAGG GCCCCCTTCC 
AGTAAGACTG GAGAGAGTGT GTCAGTGCCG TCGTCCATGG AGAGTGAGGA AGCCGCAGAA 
GTCACAGATG ACCCAATGGA GCAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp4r2 ( XM_006506008.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506071.1 Mus musculus protein phosphatase 4, regulatory subunit 2 (Ppp4r2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu37194
Clone ID Related Accession (Same CDS sequence)	XM_006506008.4 , XM_006506008.3
Accession Version	XM_006506008.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein phosphatase 4 regulatory subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506008.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTCG AGAGGCTGCA GGAGGCGCTG AAAGATTTTG AGAAGAGAGG GAAAAAGGAA 
GTTTGTCCTG TACTGGATCA GTTTCTCTGT CATGTAGCTA AAACTGGAGA AACAATGATT 
CAGTGGTCCC AATTTAAAGG CTATTTCATT TTCAAACTGG AGAAAGTGAT GGATGATTTC 
AGAACTTCAG CTCCTGAACC AAGAGGTCCT CCCAATCCTA ATGTTGAATA TATTCCCTTT 
GATGAAATGA AGGAAAGAAT ACTGAAAATT GTCACTGGAT TTAATGGTAT CCCTTTTACT 
ATTCAGCGGC TATGTGAATT GCTAACGGAT CCGAGAAGAA ACTATACAGG AACAGACAAG 
TTTCTCAGAG GAGTAGAGAA GAATGTGATG GTTGTTAGCT GCGTTTGTCC ATCCTCAGAG 
AAGAACAATT CTAATAGCTT AAATAGAATG AATGGTGTTA TGTTTCCTGG AAACTCACCA 
AACTATACTG ACAGTGATTC TTCAGCCTCT GAATCAGAAG TTTCCTTACT GAGCCCTGTT 
AAAAATAAAC ATCCAGATGA AGATGCTGTG GAGTCTGAGG AACATGAGGT GAAAAGACTG 
AAGTTTGACA AAGAAGGTGA CGTCAGAGAG ACAGCTAGCC AAACGGTGTC TGGTGAAGTC 
TCTTCAGTTA GAGCAGAGGA AACGGAAACA GCAGCTCCCC CTCCTGACAA GGACAGAGAA 
AGTCGAACCA GGCAGCACTG TACAGAAGAG GAGGAGGAGG AGGAGGAAGA AGAGGAAGAG 
GAGGAGGAAG AATCGTTTAT GACACCAAGA GAAATGGTCC CAGAAAGAAA AAATCAAGAA 
AAGGAATCTG ATGACGCCTT AACTGTGAAT GAAGAGACTT CAGAGGAGAG CCATCAGATG 
GAAGGCTCTG GTGTGTCTCC AGCTCAGACA GACTCCACTT CAGAAAGGAG TGACAGTGCA 
GGGGCCTCAA GGAGTGGCTC TGACTGCCTG GAGACACAGG AGTCAGGAGG GCCCCCTTCC 
AGTAAGACTG GAGAGAGTGT GTCAGTGCCG TCGTCCATGG AGAGTGAGGA AGCCGCAGAA 
GTCACAGATG ACCCAATGGA GCAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506071.1
CDS	251..1360
Translation	MDVERLQEALKDFEKRGKKEVCPVLDQFLCHVAKTGETMIQWSQFKGYFIFKLEKVMDDFRTSAPEPRGPPNPNVEYIPFDEMKERILKIVTGFNGIPFTIQRLCELLTDPRRNYTGTDKFLRGVEKNVMVVSCVCPSSEKNNSNSLNRMNGVMFPGNSPNYTDSDSSASESEVSLLSPVKNKHPDEDAVESEEHEVKRLKFDKEGDVRETASQTVSGEVSSVRAEETETAAPPPDKDRESRTRQHCTEEEEEEEEEEEEEEEESFMTPREMVPERKNQEKESDDALTVNEETSEESHQMEGSGVSPAQTDSTSERSDSAGASRSGSDCLETQESGGPPSSKTGESVSVPSSMESEEAAEVTDDPMEQD

Target ORF information:

RefSeq Version	XM_006506008.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 4, regulatory subunit 2 (Ppp4r2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506008.3

ORF Insert Sequence:

ATGGACGTCG AGAGGCTGCA GGAGGCGCTG AAAGATTTTG AGAAGAGAGG GAAAAAGGAA 
GTTTGTCCTG TACTGGATCA GTTTCTCTGT CATGTAGCTA AAACTGGAGA AACAATGATT 
CAGTGGTCCC AATTTAAAGG CTATTTCATT TTCAAACTGG AGAAAGTGAT GGATGATTTC 
AGAACTTCAG CTCCTGAACC AAGAGGTCCT CCCAATCCTA ATGTTGAATA TATTCCCTTT 
GATGAAATGA AGGAAAGAAT ACTGAAAATT GTCACTGGAT TTAATGGTAT CCCTTTTACT 
ATTCAGCGGC TATGTGAATT GCTAACGGAT CCGAGAAGAA ACTATACAGG AACAGACAAG 
TTTCTCAGAG GAGTAGAGAA GAATGTGATG GTTGTTAGCT GCGTTTGTCC ATCCTCAGAG 
AAGAACAATT CTAATAGCTT AAATAGAATG AATGGTGTTA TGTTTCCTGG AAACTCACCA 
AACTATACTG ACAGTGATTC TTCAGCCTCT GAATCAGAAG TTTCCTTACT GAGCCCTGTT 
AAAAATAAAC ATCCAGATGA AGATGCTGTG GAGTCTGAGG AACATGAGGT GAAAAGACTG 
AAGTTTGACA AAGAAGGTGA CGTCAGAGAG ACAGCTAGCC AAACGGTGTC TGGTGAAGTC 
TCTTCAGTTA GAGCAGAGGA AACGGAAACA GCAGCTCCCC CTCCTGACAA GGACAGAGAA 
AGTCGAACCA GGCAGCACTG TACAGAAGAG GAGGAGGAGG AGGAGGAAGA AGAGGAAGAG 
GAGGAGGAAG AATCGTTTAT GACACCAAGA GAAATGGTCC CAGAAAGAAA AAATCAAGAA 
AAGGAATCTG ATGACGCCTT AACTGTGAAT GAAGAGACTT CAGAGGAGAG CCATCAGATG 
GAAGGCTCTG GTGTGTCTCC AGCTCAGACA GACTCCACTT CAGAAAGGAG TGACAGTGCA 
GGGGCCTCAA GGAGTGGCTC TGACTGCCTG GAGACACAGG AGTCAGGAGG GCCCCCTTCC 
AGTAAGACTG GAGAGAGTGT GTCAGTGCCG TCGTCCATGG AGAGTGAGGA AGCCGCAGAA 
GTCACAGATG ACCCAATGGA GCAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Haploinsufficiency of SAMD9L, an endosome fusion facilitator, causes myeloid malignancies in mice mimicking human diseases with monosomy 7.
Cancer cell24(3)305-17(2013 Sep)
Nagamachi A,Matsui H,Asou H,Ozaki Y,Aki D,Kanai A,Takubo K,Suda T,Nakamura T,Wolff L,Honda H,Inaba T

Suppressor of MEK null (SMEK)/protein phosphatase 4 catalytic subunit (PP4C) is a key regulator of hepatic gluconeogenesis.
Proceedings of the National Academy of Sciences of the United States of America107(41)17704-9(2010 Oct)
Yoon YS,Lee MW,Ryu D,Kim JH,Ma H,Seo WY,Kim YN,Kim SS,Lee CH,Hunter T,Choi CS,Montminy MR,Koo SH

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Ppp4r2 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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