Micall2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Micall2
Entrez Gene ID	231830
Full Name	MICAL-like 2
Synonyms	A930021H16Rik,JRAB,MICAL-L2,mFLJ00139
General protein information	Preferred Names MICAL-like 2 Names MICAL-like protein 2 junctional Rab13-binding protein molecule interacting with CasL-like 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G2

mRNA and Protein(s)

mRNA	Protein	Name
XM_011240974.2	XP_011239276.1	MICAL-like protein 2 isoform X1
NM_174850.3	NP_777275.2	MICAL-like protein 2
XM_011240974.3	XP_011239276.1	MICAL-like protein 2 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Micall2	NP_777275.2
Gallus gallus (chicken)	MICALL2	XP_004945190.1
Xenopus tropicalis (tropical clawed frog)	micall2	NP_001072513.1
Homo sapiens (human)	MICALL2	NP_891554.1
	MICALL2	XP_001100629.2
Canis lupus familiaris (dog)	MICALL2	XP_005621229.1
Bos taurus (cattle)	MICALL2	NP_001092402.1
Rattus norvegicus (Norway rat)	Micall2	XP_006249037.1

Gene Ontology
Bibliography

Related articles in PubMed

EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed transport in polarized epithelial cells.
Nakajo A, Yoshimura S, Togawa H, Kunii M, Iwano T, Izumi A, Noguchi Y, Watanabe A, Goto A, Sato T, Harada A
The Journal of cell biology212(3)297-306(2016 Feb)

Junctional Rab13-binding protein (JRAB) regulates cell spreading via filamins.
Sakane A, Alamir Mahmoud Abdallah A, Nakano K, Honda K, Kitamura T, Imoto I, Matsushita N, Sasaki T
Genes to cells : devoted to molecular & cellular mechanisms18(9)810-22(2013 Sep)

Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2.
Sakane A, Honda K, Sasaki T
Molecular and cellular biology30(4)1077-87(2010 Feb)

Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions.
Nakatsuji H, Nishimura N, Yamamura R, Kanayama HO, Sasaki T
Molecular and cellular biology28(10)3324-35(2008 May)

The interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions.
Yamamura R, Nishimura N, Nakatsuji H, Arase S, Sasaki T
Molecular biology of the cell19(3)971-83(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Interaction between JRAB and filamin regulates JRABDeltaCC-induced cell spreading.
Title: Junctional Rab13-binding protein (JRAB) regulates cell spreading via filamins.

Rab13 and JRAB regulate reorganization of the actin cytoskeleton throughout epithelial junctional development.
Title: Rab13 small G protein and junctional Rab13-binding protein (JRAB) orchestrate actin cytoskeletal organization during epithelial junctional development.

show that Rab13 regulates neurite outgrowth in the rat pheochromocytoma cell line PC12 through an interaction with JRAB/MICAL-L2.
Title: Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2.

Although actinin-4 bound to JRAB/MICAL-L2 without Rab13, the actinin-4-JRAB/MICAL-L2 interaction was enhanced by Rab13 activation.
Title: Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions.

These results suggest that the interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions.
Title: The interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions.

The following Micall2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Micall2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu73581	XM_011240974.3 Latest version!	Mus musculus MICAL-like 2 (Micall2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu13156	NM_174850.3 Latest version!	Mus musculus MICAL-like 2 (Micall2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$594.30 $849.00
View Old Accession Versions >>
OMu73581	XM_011240974.2	Mus musculus MICAL-like 2 (Micall2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Micall2 ( XM_011240974.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239276.1 Mus musculus MICAL-like 2 (Micall2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73581
Clone ID Related Accession (Same CDS sequence)	XM_011240974.2 , XM_011240974.3
Accession Version	XM_011240974.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MICAL-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240974.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCCT TGAAGGTTCC AGACCGGCTG AGCATTCTCA CCTATGTGTC ACAATACTAC 
AACTACTTCC ATGGCCGGTC CCCAATTGGG GGCATGGCTG GCATAAAGAG ACCCTCATCC 
GATTCGACCG AAGAACTTTC TGGAAAGAAG GGCCTGTCCC AGCCTGCCAA GTTGCCCTCG 
CCGGCTCAAA CCCAGAGGTC ACCACTGTCC CCAGCTCGGA CAAACCCTGT GGTTCAGAGG 
AATGAAGGTG GCTCACAAAG ACCATCCCCA AAGGCTGCTC CGGGGACCGC GGGAAGCTCT 
GTCAGCAGTA TCTGTGGGGT CTGTGGCAAA CATGTTCACC TTGTGCAGCG GCACCTGGCT 
GACGGGCGCC TCTACCACCG AAGCTGCTTC AGGTGTAAAC AGTGCTCCAG CACGCTGCAC 
TCGGGGGCCT ACCGTGCCAC GGGAGAGCCC GGAGTCTTCG TCTGTACCCA CCACAGCTCA 
GAAGTCACCT CTGTGAGCCC CAAGTCATCA AACCTGGCCT CCAGGAAACC AGGGGGTGTC 
ACTGCAGACA CCAGGCCATT CGGCGTCTCA TGGACGGTTC AGGAGGCAAA TGGAGAGGGG 
ACACCACTGA GGGTCAGGAC AGCAGCATGG GAACACGCTG GAGGAAACAC AACCGCCAAA 
GGTTTTGTCC AGACGGAACT TAAGCCACCA TCTACCTCCC AAGTCCACGT GGGGAGCTCT 
GCGGGGCCCA AGCTACCCAC GATCACAGTG ACTACTACTT CTGTGACTAG CAAAGCTCTC 
ACCCATGTGA CGAATAGCTC CCCAATAGGA TGGTCATCGC CTGCCCAGAG CAGCCCAGCG 
AACTTCAACT CCCGCCCAGT TGTATCCCCA AGTGCTCGGA ACACTCACCT ACCTGGGTCC 
CAAGGGCAGA CAGCCTCAAA AGGTGTTAAG ACTCAGCTCA ACTTGAACTC AGAGTCTTCA 
AACACAGCGG TCACCCCAGC CTGGACTTCC TCAGCCTCTA AGACCCAGCA GGCCCGAGAG 
AAATTTTTCC AGACCCCTCC CTCGGCCCCA GCCCCAGCCT CAGCCCCAGC CCCAGCCCCC 
ACCAGCAAGG TCCCCACTGT AGTGACTGTC CCCACCAGCA AGGTCCCCAA TGTGGTGACT 
GCCCCCACCA GCAAGGTCCC CACTGTAGTG ACTGTCCCCA CCAGCAAGGT CCCCACTGTG 
GTGTCTGCCC CCACCAGCAA GGTCCCTACT GTGGTGTCTG CCCCCACCAG CAAGGTCCCC 
ACTGTGGTGA ATTCCACTAA TAGCAGGGTC ACCACGGTAG TAAATGCCCC CACCAGCAAG 
GTCCCCACTG TAGTGAGTGC CACAAATGGC AGGGTGCCCA CTGTGGTGAC TGCCCACACT 
GGTAGGGTCC CTGCTGTGAT GAATACCTCT GCCAGCAAAG TCTCTCCTGT TGTGGATGCC 
CCTGCCCAGG AGAGCAGCCG GGAGCAAGCA TTAAGTGTCC TTAGGAAGGC CCTTCCGGCG 
CTGACAGGAT CTGGTACCCA GGCTCCAAAC AGGTCCTTCC CAGCCACTTC CTCTGTCCTG 
GTAACCCTTC CCAAGAATGA AGTGCCACAA AAAGTTCCCT CAGACAAGCT CTCAGCTTTA 
ACCACCCAGA CTCCTAATTT CACTATAAAG CTAGAGCCTT CAGCCCCAGT GAATGTGGGC 
AATACCGCTG TATTTCTCCA GGCTGGCAAA AAGAGCCCGA GTATTTCTCC AAGGGTTGGT 
AAGACCAGCG TTGGCTCCAG GCCACAAGCA GAGGTGGCAG GAGTAAAGGG TCCAGGTCCC 
ATCTCTCAGG AAGGCCAAGA GGAGGGACCA GAAGGATGGA GGGCCCGGCT GAAGCCTGTG 
GATAAGAAAA CCCCTGCTGG GAGGTCTCTG GAGCAAAAAG AGCCAGTCCT GGCAGAGCCA 
AGGATAGGTG ACACTTCCAG GAAAGCCTCT AGTAGCTCTG ACTCCAGCGT CCATATCACC 
TTGACTTCTA TTCAGCACAA GAGGAAACCA TGCCCTGCTG GTTCCGGGCC CAGTCCTGCA 
GCCCTCTCTC CTTCTCCATC ACACCGCAAG AAACTAGCTG TCCCTCCCAG CCTTGATGTT 
TCAGCTGATT GGCTCCAGCC AGAGCCCAAG AAGCAGGAAG ATGGGACCAG GAGCTGTAAG 
GAGGAGAAGT CACCCACCAG GTGGTCAAGA GAGAGGTCTG CAGTCCTGGA CAGTGGCCTT 
GCTCCACCTG GTGAAGCTGT CACTTCCCCA GTCAGGCTCC ACCCCGACTA TATCCCCCAG 
GAGGAGCTGC AGAGGCAGCT GCAGGACATT GAGAGCCAAC TGGACGCCCT GGAGCTCAGG 
GGTGTTGAGT TGGAGAAGCG GCTGCGGGCA GCTGAGGGAG ATGCTTCTGA GGATAGCCTC 
ATGGTGGACT GGTTCCGGCT TATTCATGAG AAGCAGCTAC TGTTGAGGCT GGAATCGGAA 
CTCATGTACA AGTCCAAGGA CCAGCGCCTG GAGGAGCAGC AGCTGGACCT CCAGGGTGAG 
CTGCGCAGGC TGATGGACAA GCCAGAGGGT CTGAAGTCCC CCCAGGACCG CCAGCGGGAG 
CAGGAGCTGC TGAGCCAATA CGTGAACACC GTGAATGACC GAAGTGACAT CGTTGACTTC 
CTGGATGAGG ATCGGCTCCG GGAACAGGAA GAGGACCAAA TGCTGGAGAA TATGATCCAG 
AACCTGGGCC TCCAGAGAAA GAAGTCCAAG TCCTTCTTGT CCAAGATCTG GTCCTCGAAG 
AGCAAAAGCG GGCAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239276.1
CDS	187..2967
Translation	MVALKVPDRLSILTYVSQYYNYFHGRSPIGGMAGIKRPSSDSTEELSGKKGLSQPAKLPSPAQTQRSPLSPARTNPVVQRNEGGSQRPSPKAAPGTAGSSVSSICGVCGKHVHLVQRHLADGRLYHRSCFRCKQCSSTLHSGAYRATGEPGVFVCTHHSSEVTSVSPKSSNLASRKPGGVTADTRPFGVSWTVQEANGEGTPLRVRTAAWEHAGGNTTAKGFVQTELKPPSTSQVHVGSSAGPKLPTITVTTTSVTSKALTHVTNSSPIGWSSPAQSSPANFNSRPVVSPSARNTHLPGSQGQTASKGVKTQLNLNSESSNTAVTPAWTSSASKTQQAREKFFQTPPSAPAPASAPAPAPTSKVPTVVTVPTSKVPNVVTAPTSKVPTVVTVPTSKVPTVVSAPTSKVPTVVSAPTSKVPTVVNSTNSRVTTVVNAPTSKVPTVVSATNGRVPTVVTAHTGRVPAVMNTSASKVSPVVDAPAQESSREQALSVLRKALPALTGSGTQAPNRSFPATSSVLVTLPKNEVPQKVPSDKLSALTTQTPNFTIKLEPSAPVNVGNTAVFLQAGKKSPSISPRVGKTSVGSRPQAEVAGVKGPGPISQEGQEEGPEGWRARLKPVDKKTPAGRSLEQKEPVLAEPRIGDTSRKASSSSDSSVHITLTSIQHKRKPCPAGSGPSPAALSPSPSHRKKLAVPPSLDVSADWLQPEPKKQEDGTRSCKEEKSPTRWSRERSAVLDSGLAPPGEAVTSPVRLHPDYIPQEELQRQLQDIESQLDALELRGVELEKRLRAAEGDASEDSLMVDWFRLIHEKQLLLRLESELMYKSKDQRLEEQQLDLQGELRRLMDKPEGLKSPQDRQREQELLSQYVNTVNDRSDIVDFLDEDRLREQEEDQMLENMIQNLGLQRKKSKSFLSKIWSSKSKSGQA

Target ORF information:

RefSeq Version	XM_011240974.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus MICAL-like 2 (Micall2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240974.3

ORF Insert Sequence:

ATGGTGGCCT TGAAGGTTCC AGACCGGCTG AGCATTCTCA CCTATGTGTC ACAATACTAC 
AACTACTTCC ATGGCCGGTC CCCAATTGGG GGCATGGCTG GCATAAAGAG ACCCTCATCC 
GATTCGACCG AAGAACTTTC TGGAAAGAAG GGCCTGTCCC AGCCTGCCAA GTTGCCCTCG 
CCGGCTCAAA CCCAGAGGTC ACCACTGTCC CCAGCTCGGA CAAACCCTGT GGTTCAGAGG 
AATGAAGGTG GCTCACAAAG ACCATCCCCA AAGGCTGCTC CGGGGACCGC GGGAAGCTCT 
GTCAGCAGTA TCTGTGGGGT CTGTGGCAAA CATGTTCACC TTGTGCAGCG GCACCTGGCT 
GACGGGCGCC TCTACCACCG AAGCTGCTTC AGGTGTAAAC AGTGCTCCAG CACGCTGCAC 
TCGGGGGCCT ACCGTGCCAC GGGAGAGCCC GGAGTCTTCG TCTGTACCCA CCACAGCTCA 
GAAGTCACCT CTGTGAGCCC CAAGTCATCA AACCTGGCCT CCAGGAAACC AGGGGGTGTC 
ACTGCAGACA CCAGGCCATT CGGCGTCTCA TGGACGGTTC AGGAGGCAAA TGGAGAGGGG 
ACACCACTGA GGGTCAGGAC AGCAGCATGG GAACACGCTG GAGGAAACAC AACCGCCAAA 
GGTTTTGTCC AGACGGAACT TAAGCCACCA TCTACCTCCC AAGTCCACGT GGGGAGCTCT 
GCGGGGCCCA AGCTACCCAC GATCACAGTG ACTACTACTT CTGTGACTAG CAAAGCTCTC 
ACCCATGTGA CGAATAGCTC CCCAATAGGA TGGTCATCGC CTGCCCAGAG CAGCCCAGCG 
AACTTCAACT CCCGCCCAGT TGTATCCCCA AGTGCTCGGA ACACTCACCT ACCTGGGTCC 
CAAGGGCAGA CAGCCTCAAA AGGTGTTAAG ACTCAGCTCA ACTTGAACTC AGAGTCTTCA 
AACACAGCGG TCACCCCAGC CTGGACTTCC TCAGCCTCTA AGACCCAGCA GGCCCGAGAG 
AAATTTTTCC AGACCCCTCC CTCGGCCCCA GCCCCAGCCT CAGCCCCAGC CCCAGCCCCC 
ACCAGCAAGG TCCCCACTGT AGTGACTGTC CCCACCAGCA AGGTCCCCAA TGTGGTGACT 
GCCCCCACCA GCAAGGTCCC CACTGTAGTG ACTGTCCCCA CCAGCAAGGT CCCCACTGTG 
GTGTCTGCCC CCACCAGCAA GGTCCCTACT GTGGTGTCTG CCCCCACCAG CAAGGTCCCC 
ACTGTGGTGA ATTCCACTAA TAGCAGGGTC ACCACGGTAG TAAATGCCCC CACCAGCAAG 
GTCCCCACTG TAGTGAGTGC CACAAATGGC AGGGTGCCCA CTGTGGTGAC TGCCCACACT 
GGTAGGGTCC CTGCTGTGAT GAATACCTCT GCCAGCAAAG TCTCTCCTGT TGTGGATGCC 
CCTGCCCAGG AGAGCAGCCG GGAGCAAGCA TTAAGTGTCC TTAGGAAGGC CCTTCCGGCG 
CTGACAGGAT CTGGTACCCA GGCTCCAAAC AGGTCCTTCC CAGCCACTTC CTCTGTCCTG 
GTAACCCTTC CCAAGAATGA AGTGCCACAA AAAGTTCCCT CAGACAAGCT CTCAGCTTTA 
ACCACCCAGA CTCCTAATTT CACTATAAAG CTAGAGCCTT CAGCCCCAGT GAATGTGGGC 
AATACCGCTG TATTTCTCCA GGCTGGCAAA AAGAGCCCGA GTATTTCTCC AAGGGTTGGT 
AAGACCAGCG TTGGCTCCAG GCCACAAGCA GAGGTGGCAG GAGTAAAGGG TCCAGGTCCC 
ATCTCTCAGG AAGGCCAAGA GGAGGGACCA GAAGGATGGA GGGCCCGGCT GAAGCCTGTG 
GATAAGAAAA CCCCTGCTGG GAGGTCTCTG GAGCAAAAAG AGCCAGTCCT GGCAGAGCCA 
AGGATAGGTG ACACTTCCAG GAAAGCCTCT AGTAGCTCTG ACTCCAGCGT CCATATCACC 
TTGACTTCTA TTCAGCACAA GAGGAAACCA TGCCCTGCTG GTTCCGGGCC CAGTCCTGCA 
GCCCTCTCTC CTTCTCCATC ACACCGCAAG AAACTAGCTG TCCCTCCCAG CCTTGATGTT 
TCAGCTGATT GGCTCCAGCC AGAGCCCAAG AAGCAGGAAG ATGGGACCAG GAGCTGTAAG 
GAGGAGAAGT CACCCACCAG GTGGTCAAGA GAGAGGTCTG CAGTCCTGGA CAGTGGCCTT 
GCTCCACCTG GTGAAGCTGT CACTTCCCCA GTCAGGCTCC ACCCCGACTA TATCCCCCAG 
GAGGAGCTGC AGAGGCAGCT GCAGGACATT GAGAGCCAAC TGGACGCCCT GGAGCTCAGG 
GGTGTTGAGT TGGAGAAGCG GCTGCGGGCA GCTGAGGGAG ATGCTTCTGA GGATAGCCTC 
ATGGTGGACT GGTTCCGGCT TATTCATGAG AAGCAGCTAC TGTTGAGGCT GGAATCGGAA 
CTCATGTACA AGTCCAAGGA CCAGCGCCTG GAGGAGCAGC AGCTGGACCT CCAGGGTGAG 
CTGCGCAGGC TGATGGACAA GCCAGAGGGT CTGAAGTCCC CCCAGGACCG CCAGCGGGAG 
CAGGAGCTGC TGAGCCAATA CGTGAACACC GTGAATGACC GAAGTGACAT CGTTGACTTC 
CTGGATGAGG ATCGGCTCCG GGAACAGGAA GAGGACCAAA TGCTGGAGAA TATGATCCAG 
AACCTGGGCC TCCAGAGAAA GAAGTCCAAG TCCTTCTTGT CCAAGATCTG GTCCTCGAAG 
AGCAAAAGCG GGCAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Micall2 ( NM_174850.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_777275.2 Mus musculus MICAL-like 2 (Micall2), mRNA.

Price & Availability↑

CloneID	OMu13156
Clone ID Related Accession (Same CDS sequence)	NM_174850.3
Accession Version	NM_174850.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	MICAL-like protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY346995.1, AB182579.1 and BY700282.1. On Jul 22, 2009 this sequence version replaced NM_174850.2. CCDS Note: The coding region has been updated to extend the N-terminus and to correct mismatches versus the reference genome assembly in the coding region which results in 4 aa changes to the C-terminus of the protein. This N-terminal extension is 114aa long, is supported by available conservation data and adds a CH (calponin homology) domain (SMART). ##Evidence-Data-START## Transcript exon combination :: AK151086.1, AK164043.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGCCA TAAAGGCCCT GCAAGAATGG TGCCGGCAGC AGTGCGAGGG CTACCGGGAC 
GTGAGCATCA CCAACATGAC CACCTCGTTC CGCGATGGCC TGGCCTTCTG CGCTATCTTG 
CACCGACACC GGCCTGACCT TATAAACTTC AGTGCCCTCC GGAAGGAAAA CATTTACGAG 
AACAACAAGT TGGCCTTCCA AGTGGCTGAG GAACAGCTGG GCATCCCAGC CCTGCTAGAC 
GCTGAGGACA TGGTGGCCTT GAAGGTTCCA GACCGGCTGA GCATTCTCAC CTATGTGTCA 
CAATACTACA ACTACTTCCA TGGCCGGTCC CCAATTGGGG GCATGGCTGG CATAAAGAGA 
CCCTCATCCG ATTCGACCGA AGAACTTTCT GGAAAGAAGG GCCTGTCCCA GCCTGCCAAG 
TTGCCCTCGC CGGCTCAAAC CCAGAGGTCA CCACTGTCCC CAGCTCGGAC AAACCCTGTG 
GTTCAGAGGA ATGAAGGTGG CTCACAAAGA CCATCCCCAA AGGCTGCTCC GGGGACCGCG 
GGAAGCTCTG TCAGCAGTAT CTGTGGGGTC TGTGGCAAAC ATGTTCACCT TGTGCAGCGG 
CACCTGGCTG ACGGGCGCCT CTACCACCGA AGCTGCTTCA GGTGTAAACA GTGCTCCAGC 
ACGCTGCACT CGGGGGCCTA CCGTGCCACG GGAGAGCCCG GAGTCTTCGT CTGTACCCAC 
CACAGCTCAG AAGTCACCTC TGTGAGCCCC AAGTCATCAA ACCTGGCCTC CAGGAAACCA 
GGGGGTGTCA CTGCAGACAC CAGGCCATTC GGCGTCTCAT GGACGGTTCA GGAGGCAAAT 
GGAGAGGGGA CACCACTGAG GGTCAGGACA GCAGCATGGG AACACGCTGG AGGAAACACA 
ACCGCCAAAG GTTTTGTCCA GACGGAACTT AAGCCACCAT CTACCTCCCA AGTCCACGTG 
GGGAGCTCTG CGGGGCCCAA GCTACCCACG ATCACAGTGA CTACTACTTC TGTGACTAGC 
AAAGCTCTCA CCCATGTGAC GAATAGCTCC CCAATAGGAT GGTCATCGCC TGCCCAGAGC 
AGCCCAGCGA ACTTCAACTC CCGCCCAGTT GTATCCCCAA GTGCTCGGAA CACTCACCTA 
CCTGGGTCCC AAGGGCAGAC AGCCTCAAAA GGTGTTAAGA CTCAGCTCAA CTTGAACTCA 
GAGTCTTCAA ACACAGCGGT CACCCCAGCC TGGACTTCCT CAGCCTCTAA GACCCAGCAG 
GCCCGAGAGA AATTTTTCCA GACCCCTCCC TCGGCCCCAG CCCCAGCCTC AGCCCCAGCC 
CCAGCCCCCA CCAGCAAGGT CCCCACTGTA GTGACTGTCC CCACCAGCAA GGTCCCCAAT 
GTGGTGACTG CCCCCACCAG CAAGGTCCCC ACTGTAGTGA CTGTCCCCAC CAGCAAGGTC 
CCCACTGTGG TGTCTGCCCC CACCAGCAAG GTCCCTACTG TGGTGTCTGC CCCCACCAGC 
AAGGTCCCCA CTGTGGTGAA TTCCACTAAT AGCAGGGTCA CCACGGTAGT AAATGCCCCC 
ACCAGCAAGG TCCCCACTGT AGTGAGTGCC ACAAATGGCA GGGTGCCCAC TGTGGTGACT 
GCCCACACTG GTAGGGTCCC TGCTGTGATG AATACCTCTG CCAGCAAAGT CTCTCCTGTT 
GTGGATGCCC CTGCCCAGGA GAGCAGCCGG GAGCAAGCAT TAAGTGTCCT TAGGAAGGCC 
CTTCCGGCGC TGACAGGATC TGGTACCCAG GCTCCAAACA GGTCCTTCCC AGCCACTTCC 
TCTGTCCTGG TAACCCTTCC CAAGAATGAA GTGCCACAAA AAGTTCCCTC AGACAAGCTC 
TCAGCTTTAA CCACCCAGAC TCCTAATTTC ACTATAAAGC TAGAGCCTTC AGCCCCAGTG 
AATGTGGGCA ATACCGCTGT ATTTCTCCAG GCTGGCAAAA AGAGCCCGAG TATTTCTCCA 
AGGGTTGGTA AGACCAGCGT TGGCTCCAGG CCACAAGCAG AGGTGGCAGG AGTAAAGGGT 
CCAGGTCCCA TCTCTCAGGA AGGCCAAGAG GAGGGACCAG AAGGATGGAG GGCCCGGCTG 
AAGCCTGTGG ATAAGAAAAC CCCTGCTGGG AGGTCTCTGG AGCAAAAAGA GCCAGTCCTG 
GCAGAGCCAA GGATAGGTGA CACTTCCAGG AAAGCCTCTA GTAGCTCTGA CTCCAGCGTC 
CATATCACCT TGACTTCTAT TCAGCACAAG AGGAAACCAT GCCCTGCTGG TTCCGGGCCC 
AGTCCTGCAG CCCTCTCTCC TTCTCCATCA CACCGCAAGA AACTAGCTGT CCCTCCCAGC 
CTTGATGTTT CAGCTGATTG GCTCCAGCCA GAGCCCAAGA AGCAGGAAGA TGGGACCAGG 
AGCTGTAAGG AGGAGAAGTC ACCCACCAGG TGGTCAAGAG AGAGGTCTGC AGTCCTGGAC 
AGTGGCCTTG CTCCACCTGG TGAAGCTGTC ACTTCCCCAG TCAGGCTCCA CCCCGACTAT 
ATCCCCCAGG AGGAGCTGCA GAGGCAGCTG CAGGACATTG AGAGCCAACT GGACGCCCTG 
GAGCTCAGGG GTGTTGAGTT GGAGAAGCGG CTGCGGGCAG CTGAGGGAGA TGCTTCTGAG 
GATAGCCTCA TGGTGGACTG GTTCCGGCTT ATTCATGAGA AGCAGCTACT GTTGAGGCTG 
GAATCGGAAC TCATGTACAA GTCCAAGGAC CAGCGCCTGG AGGAGCAGCA GCTGGACCTC 
CAGGGTGAGC TGCGCAGGCT GATGGACAAG CCAGAGGGTC TGAAGTCCCC CCAGGACCGC 
CAGCGGGAGC AGGAGCTGCT GAGCCAATAC GTGAACACCG TGAATGACCG AAGTGACATC 
GTTGACTTCC TGGATGAGGA TCGGCTCCGG GAACAGGAAG AGGACCAAAT GCTGGAGAAT 
ATGATCCAGA ACCTGGGCCT CCAGAGAAAG AAGTCCAAGT CCTTCTTGTC CAAGATCTGG 
TCCTCGAAGA GCAAAAGCGG GCAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777275.2
CDS	174..3203
Translation	MAAIKALQEWCRQQCEGYRDVSITNMTTSFRDGLAFCAILHRHRPDLINFSALRKENIYENNKLAFQVAEEQLGIPALLDAEDMVALKVPDRLSILTYVSQYYNYFHGRSPIGGMAGIKRPSSDSTEELSGKKGLSQPAKLPSPAQTQRSPLSPARTNPVVQRNEGGSQRPSPKAAPGTAGSSVSSICGVCGKHVHLVQRHLADGRLYHRSCFRCKQCSSTLHSGAYRATGEPGVFVCTHHSSEVTSVSPKSSNLASRKPGGVTADTRPFGVSWTVQEANGEGTPLRVRTAAWEHAGGNTTAKGFVQTELKPPSTSQVHVGSSAGPKLPTITVTTTSVTSKALTHVTNSSPIGWSSPAQSSPANFNSRPVVSPSARNTHLPGSQGQTASKGVKTQLNLNSESSNTAVTPAWTSSASKTQQAREKFFQTPPSAPAPASAPAPAPTSKVPTVVTVPTSKVPNVVTAPTSKVPTVVTVPTSKVPTVVSAPTSKVPTVVSAPTSKVPTVVNSTNSRVTTVVNAPTSKVPTVVSATNGRVPTVVTAHTGRVPAVMNTSASKVSPVVDAPAQESSREQALSVLRKALPALTGSGTQAPNRSFPATSSVLVTLPKNEVPQKVPSDKLSALTTQTPNFTIKLEPSAPVNVGNTAVFLQAGKKSPSISPRVGKTSVGSRPQAEVAGVKGPGPISQEGQEEGPEGWRARLKPVDKKTPAGRSLEQKEPVLAEPRIGDTSRKASSSSDSSVHITLTSIQHKRKPCPAGSGPSPAALSPSPSHRKKLAVPPSLDVSADWLQPEPKKQEDGTRSCKEEKSPTRWSRERSAVLDSGLAPPGEAVTSPVRLHPDYIPQEELQRQLQDIESQLDALELRGVELEKRLRAAEGDASEDSLMVDWFRLIHEKQLLLRLESELMYKSKDQRLEEQQLDLQGELRRLMDKPEGLKSPQDRQREQELLSQYVNTVNDRSDIVDFLDEDRLREQEEDQMLENMIQNLGLQRKKSKSFLSKIWSSKSKSGQA

Target ORF information:

RefSeq Version	NM_174850.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus MICAL-like 2 (Micall2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174850.3

ORF Insert Sequence:

ATGGCCGCCA TAAAGGCCCT GCAAGAATGG TGCCGGCAGC AGTGCGAGGG CTACCGGGAC 
GTGAGCATCA CCAACATGAC CACCTCGTTC CGCGATGGCC TGGCCTTCTG CGCTATCTTG 
CACCGACACC GGCCTGACCT TATAAACTTC AGTGCCCTCC GGAAGGAAAA CATTTACGAG 
AACAACAAGT TGGCCTTCCA AGTGGCTGAG GAACAGCTGG GCATCCCAGC CCTGCTAGAC 
GCTGAGGACA TGGTGGCCTT GAAGGTTCCA GACCGGCTGA GCATTCTCAC CTATGTGTCA 
CAATACTACA ACTACTTCCA TGGCCGGTCC CCAATTGGGG GCATGGCTGG CATAAAGAGA 
CCCTCATCCG ATTCGACCGA AGAACTTTCT GGAAAGAAGG GCCTGTCCCA GCCTGCCAAG 
TTGCCCTCGC CGGCTCAAAC CCAGAGGTCA CCACTGTCCC CAGCTCGGAC AAACCCTGTG 
GTTCAGAGGA ATGAAGGTGG CTCACAAAGA CCATCCCCAA AGGCTGCTCC GGGGACCGCG 
GGAAGCTCTG TCAGCAGTAT CTGTGGGGTC TGTGGCAAAC ATGTTCACCT TGTGCAGCGG 
CACCTGGCTG ACGGGCGCCT CTACCACCGA AGCTGCTTCA GGTGTAAACA GTGCTCCAGC 
ACGCTGCACT CGGGGGCCTA CCGTGCCACG GGAGAGCCCG GAGTCTTCGT CTGTACCCAC 
CACAGCTCAG AAGTCACCTC TGTGAGCCCC AAGTCATCAA ACCTGGCCTC CAGGAAACCA 
GGGGGTGTCA CTGCAGACAC CAGGCCATTC GGCGTCTCAT GGACGGTTCA GGAGGCAAAT 
GGAGAGGGGA CACCACTGAG GGTCAGGACA GCAGCATGGG AACACGCTGG AGGAAACACA 
ACCGCCAAAG GTTTTGTCCA GACGGAACTT AAGCCACCAT CTACCTCCCA AGTCCACGTG 
GGGAGCTCTG CGGGGCCCAA GCTACCCACG ATCACAGTGA CTACTACTTC TGTGACTAGC 
AAAGCTCTCA CCCATGTGAC GAATAGCTCC CCAATAGGAT GGTCATCGCC TGCCCAGAGC 
AGCCCAGCGA ACTTCAACTC CCGCCCAGTT GTATCCCCAA GTGCTCGGAA CACTCACCTA 
CCTGGGTCCC AAGGGCAGAC AGCCTCAAAA GGTGTTAAGA CTCAGCTCAA CTTGAACTCA 
GAGTCTTCAA ACACAGCGGT CACCCCAGCC TGGACTTCCT CAGCCTCTAA GACCCAGCAG 
GCCCGAGAGA AATTTTTCCA GACCCCTCCC TCGGCCCCAG CCCCAGCCTC AGCCCCAGCC 
CCAGCCCCCA CCAGCAAGGT CCCCACTGTA GTGACTGTCC CCACCAGCAA GGTCCCCAAT 
GTGGTGACTG CCCCCACCAG CAAGGTCCCC ACTGTAGTGA CTGTCCCCAC CAGCAAGGTC 
CCCACTGTGG TGTCTGCCCC CACCAGCAAG GTCCCTACTG TGGTGTCTGC CCCCACCAGC 
AAGGTCCCCA CTGTGGTGAA TTCCACTAAT AGCAGGGTCA CCACGGTAGT AAATGCCCCC 
ACCAGCAAGG TCCCCACTGT AGTGAGTGCC ACAAATGGCA GGGTGCCCAC TGTGGTGACT 
GCCCACACTG GTAGGGTCCC TGCTGTGATG AATACCTCTG CCAGCAAAGT CTCTCCTGTT 
GTGGATGCCC CTGCCCAGGA GAGCAGCCGG GAGCAAGCAT TAAGTGTCCT TAGGAAGGCC 
CTTCCGGCGC TGACAGGATC TGGTACCCAG GCTCCAAACA GGTCCTTCCC AGCCACTTCC 
TCTGTCCTGG TAACCCTTCC CAAGAATGAA GTGCCACAAA AAGTTCCCTC AGACAAGCTC 
TCAGCTTTAA CCACCCAGAC TCCTAATTTC ACTATAAAGC TAGAGCCTTC AGCCCCAGTG 
AATGTGGGCA ATACCGCTGT ATTTCTCCAG GCTGGCAAAA AGAGCCCGAG TATTTCTCCA 
AGGGTTGGTA AGACCAGCGT TGGCTCCAGG CCACAAGCAG AGGTGGCAGG AGTAAAGGGT 
CCAGGTCCCA TCTCTCAGGA AGGCCAAGAG GAGGGACCAG AAGGATGGAG GGCCCGGCTG 
AAGCCTGTGG ATAAGAAAAC CCCTGCTGGG AGGTCTCTGG AGCAAAAAGA GCCAGTCCTG 
GCAGAGCCAA GGATAGGTGA CACTTCCAGG AAAGCCTCTA GTAGCTCTGA CTCCAGCGTC 
CATATCACCT TGACTTCTAT TCAGCACAAG AGGAAACCAT GCCCTGCTGG TTCCGGGCCC 
AGTCCTGCAG CCCTCTCTCC TTCTCCATCA CACCGCAAGA AACTAGCTGT CCCTCCCAGC 
CTTGATGTTT CAGCTGATTG GCTCCAGCCA GAGCCCAAGA AGCAGGAAGA TGGGACCAGG 
AGCTGTAAGG AGGAGAAGTC ACCCACCAGG TGGTCAAGAG AGAGGTCTGC AGTCCTGGAC 
AGTGGCCTTG CTCCACCTGG TGAAGCTGTC ACTTCCCCAG TCAGGCTCCA CCCCGACTAT 
ATCCCCCAGG AGGAGCTGCA GAGGCAGCTG CAGGACATTG AGAGCCAACT GGACGCCCTG 
GAGCTCAGGG GTGTTGAGTT GGAGAAGCGG CTGCGGGCAG CTGAGGGAGA TGCTTCTGAG 
GATAGCCTCA TGGTGGACTG GTTCCGGCTT ATTCATGAGA AGCAGCTACT GTTGAGGCTG 
GAATCGGAAC TCATGTACAA GTCCAAGGAC CAGCGCCTGG AGGAGCAGCA GCTGGACCTC 
CAGGGTGAGC TGCGCAGGCT GATGGACAAG CCAGAGGGTC TGAAGTCCCC CCAGGACCGC 
CAGCGGGAGC AGGAGCTGCT GAGCCAATAC GTGAACACCG TGAATGACCG AAGTGACATC 
GTTGACTTCC TGGATGAGGA TCGGCTCCGG GAACAGGAAG AGGACCAAAT GCTGGAGAAT 
ATGATCCAGA ACCTGGGCCT CCAGAGAAAG AAGTCCAAGT CCTTCTTGTC CAAGATCTGG 
TCCTCGAAGA GCAAAAGCGG GCAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Micall2 ( XM_011240974.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239276.1 Mus musculus MICAL-like 2 (Micall2), transcript variant X1, mRNA.

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CloneID	OMu73581
Clone ID Related Accession (Same CDS sequence)	XM_011240974.2 , XM_011240974.3
Accession Version	XM_011240974.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MICAL-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCCT TGAAGGTTCC AGACCGGCTG AGCATTCTCA CCTATGTGTC ACAATACTAC 
AACTACTTCC ATGGCCGGTC CCCAATTGGG GGCATGGCTG GCATAAAGAG ACCCTCATCC 
GATTCGACCG AAGAACTTTC TGGAAAGAAG GGCCTGTCCC AGCCTGCCAA GTTGCCCTCG 
CCGGCTCAAA CCCAGAGGTC ACCACTGTCC CCAGCTCGGA CAAACCCTGT GGTTCAGAGG 
AATGAAGGTG GCTCACAAAG ACCATCCCCA AAGGCTGCTC CGGGGACCGC GGGAAGCTCT 
GTCAGCAGTA TCTGTGGGGT CTGTGGCAAA CATGTTCACC TTGTGCAGCG GCACCTGGCT 
GACGGGCGCC TCTACCACCG AAGCTGCTTC AGGTGTAAAC AGTGCTCCAG CACGCTGCAC 
TCGGGGGCCT ACCGTGCCAC GGGAGAGCCC GGAGTCTTCG TCTGTACCCA CCACAGCTCA 
GAAGTCACCT CTGTGAGCCC CAAGTCATCA AACCTGGCCT CCAGGAAACC AGGGGGTGTC 
ACTGCAGACA CCAGGCCATT CGGCGTCTCA TGGACGGTTC AGGAGGCAAA TGGAGAGGGG 
ACACCACTGA GGGTCAGGAC AGCAGCATGG GAACACGCTG GAGGAAACAC AACCGCCAAA 
GGTTTTGTCC AGACGGAACT TAAGCCACCA TCTACCTCCC AAGTCCACGT GGGGAGCTCT 
GCGGGGCCCA AGCTACCCAC GATCACAGTG ACTACTACTT CTGTGACTAG CAAAGCTCTC 
ACCCATGTGA CGAATAGCTC CCCAATAGGA TGGTCATCGC CTGCCCAGAG CAGCCCAGCG 
AACTTCAACT CCCGCCCAGT TGTATCCCCA AGTGCTCGGA ACACTCACCT ACCTGGGTCC 
CAAGGGCAGA CAGCCTCAAA AGGTGTTAAG ACTCAGCTCA ACTTGAACTC AGAGTCTTCA 
AACACAGCGG TCACCCCAGC CTGGACTTCC TCAGCCTCTA AGACCCAGCA GGCCCGAGAG 
AAATTTTTCC AGACCCCTCC CTCGGCCCCA GCCCCAGCCT CAGCCCCAGC CCCAGCCCCC 
ACCAGCAAGG TCCCCACTGT AGTGACTGTC CCCACCAGCA AGGTCCCCAA TGTGGTGACT 
GCCCCCACCA GCAAGGTCCC CACTGTAGTG ACTGTCCCCA CCAGCAAGGT CCCCACTGTG 
GTGTCTGCCC CCACCAGCAA GGTCCCTACT GTGGTGTCTG CCCCCACCAG CAAGGTCCCC 
ACTGTGGTGA ATTCCACTAA TAGCAGGGTC ACCACGGTAG TAAATGCCCC CACCAGCAAG 
GTCCCCACTG TAGTGAGTGC CACAAATGGC AGGGTGCCCA CTGTGGTGAC TGCCCACACT 
GGTAGGGTCC CTGCTGTGAT GAATACCTCT GCCAGCAAAG TCTCTCCTGT TGTGGATGCC 
CCTGCCCAGG AGAGCAGCCG GGAGCAAGCA TTAAGTGTCC TTAGGAAGGC CCTTCCGGCG 
CTGACAGGAT CTGGTACCCA GGCTCCAAAC AGGTCCTTCC CAGCCACTTC CTCTGTCCTG 
GTAACCCTTC CCAAGAATGA AGTGCCACAA AAAGTTCCCT CAGACAAGCT CTCAGCTTTA 
ACCACCCAGA CTCCTAATTT CACTATAAAG CTAGAGCCTT CAGCCCCAGT GAATGTGGGC 
AATACCGCTG TATTTCTCCA GGCTGGCAAA AAGAGCCCGA GTATTTCTCC AAGGGTTGGT 
AAGACCAGCG TTGGCTCCAG GCCACAAGCA GAGGTGGCAG GAGTAAAGGG TCCAGGTCCC 
ATCTCTCAGG AAGGCCAAGA GGAGGGACCA GAAGGATGGA GGGCCCGGCT GAAGCCTGTG 
GATAAGAAAA CCCCTGCTGG GAGGTCTCTG GAGCAAAAAG AGCCAGTCCT GGCAGAGCCA 
AGGATAGGTG ACACTTCCAG GAAAGCCTCT AGTAGCTCTG ACTCCAGCGT CCATATCACC 
TTGACTTCTA TTCAGCACAA GAGGAAACCA TGCCCTGCTG GTTCCGGGCC CAGTCCTGCA 
GCCCTCTCTC CTTCTCCATC ACACCGCAAG AAACTAGCTG TCCCTCCCAG CCTTGATGTT 
TCAGCTGATT GGCTCCAGCC AGAGCCCAAG AAGCAGGAAG ATGGGACCAG GAGCTGTAAG 
GAGGAGAAGT CACCCACCAG GTGGTCAAGA GAGAGGTCTG CAGTCCTGGA CAGTGGCCTT 
GCTCCACCTG GTGAAGCTGT CACTTCCCCA GTCAGGCTCC ACCCCGACTA TATCCCCCAG 
GAGGAGCTGC AGAGGCAGCT GCAGGACATT GAGAGCCAAC TGGACGCCCT GGAGCTCAGG 
GGTGTTGAGT TGGAGAAGCG GCTGCGGGCA GCTGAGGGAG ATGCTTCTGA GGATAGCCTC 
ATGGTGGACT GGTTCCGGCT TATTCATGAG AAGCAGCTAC TGTTGAGGCT GGAATCGGAA 
CTCATGTACA AGTCCAAGGA CCAGCGCCTG GAGGAGCAGC AGCTGGACCT CCAGGGTGAG 
CTGCGCAGGC TGATGGACAA GCCAGAGGGT CTGAAGTCCC CCCAGGACCG CCAGCGGGAG 
CAGGAGCTGC TGAGCCAATA CGTGAACACC GTGAATGACC GAAGTGACAT CGTTGACTTC 
CTGGATGAGG ATCGGCTCCG GGAACAGGAA GAGGACCAAA TGCTGGAGAA TATGATCCAG 
AACCTGGGCC TCCAGAGAAA GAAGTCCAAG TCCTTCTTGT CCAAGATCTG GTCCTCGAAG 
AGCAAAAGCG GGCAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239276.1
CDS	184..2964
Translation	MVALKVPDRLSILTYVSQYYNYFHGRSPIGGMAGIKRPSSDSTEELSGKKGLSQPAKLPSPAQTQRSPLSPARTNPVVQRNEGGSQRPSPKAAPGTAGSSVSSICGVCGKHVHLVQRHLADGRLYHRSCFRCKQCSSTLHSGAYRATGEPGVFVCTHHSSEVTSVSPKSSNLASRKPGGVTADTRPFGVSWTVQEANGEGTPLRVRTAAWEHAGGNTTAKGFVQTELKPPSTSQVHVGSSAGPKLPTITVTTTSVTSKALTHVTNSSPIGWSSPAQSSPANFNSRPVVSPSARNTHLPGSQGQTASKGVKTQLNLNSESSNTAVTPAWTSSASKTQQAREKFFQTPPSAPAPASAPAPAPTSKVPTVVTVPTSKVPNVVTAPTSKVPTVVTVPTSKVPTVVSAPTSKVPTVVSAPTSKVPTVVNSTNSRVTTVVNAPTSKVPTVVSATNGRVPTVVTAHTGRVPAVMNTSASKVSPVVDAPAQESSREQALSVLRKALPALTGSGTQAPNRSFPATSSVLVTLPKNEVPQKVPSDKLSALTTQTPNFTIKLEPSAPVNVGNTAVFLQAGKKSPSISPRVGKTSVGSRPQAEVAGVKGPGPISQEGQEEGPEGWRARLKPVDKKTPAGRSLEQKEPVLAEPRIGDTSRKASSSSDSSVHITLTSIQHKRKPCPAGSGPSPAALSPSPSHRKKLAVPPSLDVSADWLQPEPKKQEDGTRSCKEEKSPTRWSRERSAVLDSGLAPPGEAVTSPVRLHPDYIPQEELQRQLQDIESQLDALELRGVELEKRLRAAEGDASEDSLMVDWFRLIHEKQLLLRLESELMYKSKDQRLEEQQLDLQGELRRLMDKPEGLKSPQDRQREQELLSQYVNTVNDRSDIVDFLDEDRLREQEEDQMLENMIQNLGLQRKKSKSFLSKIWSSKSKSGQA

Target ORF information:

RefSeq Version	XM_011240974.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus MICAL-like 2 (Micall2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240974.2

ORF Insert Sequence:

ATGGTGGCCT TGAAGGTTCC AGACCGGCTG AGCATTCTCA CCTATGTGTC ACAATACTAC 
AACTACTTCC ATGGCCGGTC CCCAATTGGG GGCATGGCTG GCATAAAGAG ACCCTCATCC 
GATTCGACCG AAGAACTTTC TGGAAAGAAG GGCCTGTCCC AGCCTGCCAA GTTGCCCTCG 
CCGGCTCAAA CCCAGAGGTC ACCACTGTCC CCAGCTCGGA CAAACCCTGT GGTTCAGAGG 
AATGAAGGTG GCTCACAAAG ACCATCCCCA AAGGCTGCTC CGGGGACCGC GGGAAGCTCT 
GTCAGCAGTA TCTGTGGGGT CTGTGGCAAA CATGTTCACC TTGTGCAGCG GCACCTGGCT 
GACGGGCGCC TCTACCACCG AAGCTGCTTC AGGTGTAAAC AGTGCTCCAG CACGCTGCAC 
TCGGGGGCCT ACCGTGCCAC GGGAGAGCCC GGAGTCTTCG TCTGTACCCA CCACAGCTCA 
GAAGTCACCT CTGTGAGCCC CAAGTCATCA AACCTGGCCT CCAGGAAACC AGGGGGTGTC 
ACTGCAGACA CCAGGCCATT CGGCGTCTCA TGGACGGTTC AGGAGGCAAA TGGAGAGGGG 
ACACCACTGA GGGTCAGGAC AGCAGCATGG GAACACGCTG GAGGAAACAC AACCGCCAAA 
GGTTTTGTCC AGACGGAACT TAAGCCACCA TCTACCTCCC AAGTCCACGT GGGGAGCTCT 
GCGGGGCCCA AGCTACCCAC GATCACAGTG ACTACTACTT CTGTGACTAG CAAAGCTCTC 
ACCCATGTGA CGAATAGCTC CCCAATAGGA TGGTCATCGC CTGCCCAGAG CAGCCCAGCG 
AACTTCAACT CCCGCCCAGT TGTATCCCCA AGTGCTCGGA ACACTCACCT ACCTGGGTCC 
CAAGGGCAGA CAGCCTCAAA AGGTGTTAAG ACTCAGCTCA ACTTGAACTC AGAGTCTTCA 
AACACAGCGG TCACCCCAGC CTGGACTTCC TCAGCCTCTA AGACCCAGCA GGCCCGAGAG 
AAATTTTTCC AGACCCCTCC CTCGGCCCCA GCCCCAGCCT CAGCCCCAGC CCCAGCCCCC 
ACCAGCAAGG TCCCCACTGT AGTGACTGTC CCCACCAGCA AGGTCCCCAA TGTGGTGACT 
GCCCCCACCA GCAAGGTCCC CACTGTAGTG ACTGTCCCCA CCAGCAAGGT CCCCACTGTG 
GTGTCTGCCC CCACCAGCAA GGTCCCTACT GTGGTGTCTG CCCCCACCAG CAAGGTCCCC 
ACTGTGGTGA ATTCCACTAA TAGCAGGGTC ACCACGGTAG TAAATGCCCC CACCAGCAAG 
GTCCCCACTG TAGTGAGTGC CACAAATGGC AGGGTGCCCA CTGTGGTGAC TGCCCACACT 
GGTAGGGTCC CTGCTGTGAT GAATACCTCT GCCAGCAAAG TCTCTCCTGT TGTGGATGCC 
CCTGCCCAGG AGAGCAGCCG GGAGCAAGCA TTAAGTGTCC TTAGGAAGGC CCTTCCGGCG 
CTGACAGGAT CTGGTACCCA GGCTCCAAAC AGGTCCTTCC CAGCCACTTC CTCTGTCCTG 
GTAACCCTTC CCAAGAATGA AGTGCCACAA AAAGTTCCCT CAGACAAGCT CTCAGCTTTA 
ACCACCCAGA CTCCTAATTT CACTATAAAG CTAGAGCCTT CAGCCCCAGT GAATGTGGGC 
AATACCGCTG TATTTCTCCA GGCTGGCAAA AAGAGCCCGA GTATTTCTCC AAGGGTTGGT 
AAGACCAGCG TTGGCTCCAG GCCACAAGCA GAGGTGGCAG GAGTAAAGGG TCCAGGTCCC 
ATCTCTCAGG AAGGCCAAGA GGAGGGACCA GAAGGATGGA GGGCCCGGCT GAAGCCTGTG 
GATAAGAAAA CCCCTGCTGG GAGGTCTCTG GAGCAAAAAG AGCCAGTCCT GGCAGAGCCA 
AGGATAGGTG ACACTTCCAG GAAAGCCTCT AGTAGCTCTG ACTCCAGCGT CCATATCACC 
TTGACTTCTA TTCAGCACAA GAGGAAACCA TGCCCTGCTG GTTCCGGGCC CAGTCCTGCA 
GCCCTCTCTC CTTCTCCATC ACACCGCAAG AAACTAGCTG TCCCTCCCAG CCTTGATGTT 
TCAGCTGATT GGCTCCAGCC AGAGCCCAAG AAGCAGGAAG ATGGGACCAG GAGCTGTAAG 
GAGGAGAAGT CACCCACCAG GTGGTCAAGA GAGAGGTCTG CAGTCCTGGA CAGTGGCCTT 
GCTCCACCTG GTGAAGCTGT CACTTCCCCA GTCAGGCTCC ACCCCGACTA TATCCCCCAG 
GAGGAGCTGC AGAGGCAGCT GCAGGACATT GAGAGCCAAC TGGACGCCCT GGAGCTCAGG 
GGTGTTGAGT TGGAGAAGCG GCTGCGGGCA GCTGAGGGAG ATGCTTCTGA GGATAGCCTC 
ATGGTGGACT GGTTCCGGCT TATTCATGAG AAGCAGCTAC TGTTGAGGCT GGAATCGGAA 
CTCATGTACA AGTCCAAGGA CCAGCGCCTG GAGGAGCAGC AGCTGGACCT CCAGGGTGAG 
CTGCGCAGGC TGATGGACAA GCCAGAGGGT CTGAAGTCCC CCCAGGACCG CCAGCGGGAG 
CAGGAGCTGC TGAGCCAATA CGTGAACACC GTGAATGACC GAAGTGACAT CGTTGACTTC 
CTGGATGAGG ATCGGCTCCG GGAACAGGAA GAGGACCAAA TGCTGGAGAA TATGATCCAG 
AACCTGGGCC TCCAGAGAAA GAAGTCCAAG TCCTTCTTGT CCAAGATCTG GTCCTCGAAG 
AGCAAAAGCG GGCAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed transport in polarized epithelial cells.
The Journal of cell biology212(3)297-306(2016 Feb)
Nakajo A,Yoshimura S,Togawa H,Kunii M,Iwano T,Izumi A,Noguchi Y,Watanabe A,Goto A,Sato T,Harada A

Junctional Rab13-binding protein (JRAB) regulates cell spreading via filamins.
Genes to cells : devoted to molecular & cellular mechanisms18(9)810-22(2013 Sep)
Sakane A,Alamir Mahmoud Abdallah A,Nakano K,Honda K,Kitamura T,Imoto I,Matsushita N,Sasaki T

Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2.
Molecular and cellular biology30(4)1077-87(2010 Feb)
Sakane A,Honda K,Sasaki T

Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions.
Molecular and cellular biology28(10)3324-35(2008 May)
Nakatsuji H,Nishimura N,Yamamura R,Kanayama HO,Sasaki T

The interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions.
Molecular biology of the cell19(3)971-83(2008 Mar)
Yamamura R,Nishimura N,Nakatsuji H,Arase S,Sasaki T