Lrch4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lrch4
Entrez Gene ID	231798
Full Name	leucine-rich repeats and calponin homology (CH) domain containing 4
Synonyms	2810008P14Rik,2900069C24Rik,AI558103,LRN,LRRN4,SAP25,mFLJ00248
General protein information	Preferred Names leucine-rich repeats and calponin homology (CH) domain containing 4 Names leucine-rich repeat and calponin homology domain-containing protein 4 leucine rich repeat protein 4, neuronal
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001168652.1	NP_001162123.1	leucine-rich repeat and calponin homology domain-containing protein 4 isoform 2
NM_146164.2	NP_666276.1	leucine-rich repeat and calponin homology domain-containing protein 4 isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LRCH4	NP_001076919.1
Rattus norvegicus (Norway rat)	Lrch4	NP_001121023.1
Mus musculus (house mouse)	Lrch4	NP_666276.1
Danio rerio (zebrafish)	LOC100535148	XP_003199053.2
Homo sapiens (human)	LRCH4	NP_002310.2
Danio rerio (zebrafish)	lrch4	XP_003199055.1
Pan troglodytes (chimpanzee)	LRCH4	XP_003318699.1
Canis lupus familiaris (dog)	LRCH4	XP_546954.4

Gene Ontology
Bibliography

Related articles in PubMed

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Evsikov AV, Graber JH, Brockman JM, Hampl A, Holbrook AE, Singh P, Eppig JJ, Solter D, Knowles BB
Genes & development20(19)2713-27(2006 Oct)

Identification and characterization of SAP25, a novel component of the mSin3 corepressor complex.
Shiio Y, Rose DW, Aur R, Donohoe S, Aebersold R, Eisenman RN
Molecular and cellular biology26(4)1386-97(2006 Feb)

Transcriptome analysis of mouse stem cells and early embryos.
Sharov AA, Piao Y, Matoba R, Dudekula DB, Qian Y, VanBuren V, Falco G, Martin PR, Stagg CA, Bassey UC, Wang Y, Carter MG, Hamatani T, Aiba K, Akutsu H, Sharova L, Tanaka TS, Kimber WL, Yoshikawa T, Jaradat SA, Pantano S, Nagaraja R, Boheler KR, Taub D, Hodes RJ, Longo DL, Schlessinger D, Keller J, Klotz E, Kelsoe G, Umezawa A, Vescovi AL, Rossant J, Kunath T, Hogan BL, Curci A, D'Urso M, Kelso J, Hide W, Ko MS
PLoS biology1(3)E74(2003 Dec)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SAP25 is a novel core component of the Sin3 corepressor complex whose subcellular location is regulated by PML.
Title: Identification and characterization of SAP25, a novel component of the mSin3 corepressor complex.

The following Lrch4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lrch4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11563	NM_001168652.1 Latest version!	Mus musculus leucine-rich repeats and calponin homology (CH) domain containing 4 (Lrch4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00
OMu12279	NM_146164.2 Latest version!	Mus musculus leucine-rich repeats and calponin homology (CH) domain containing 4 (Lrch4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$398.30 $569.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lrch4 ( NM_001168652.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001162123.1 Mus musculus leucine-rich repeats and calponin homology (CH) domain containing 4 (Lrch4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu11563
Clone ID Related Accession (Same CDS sequence)	NM_001168652.1
Accession Version	NM_001168652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Mus musculus(house mouse)
Product	leucine-rich repeat and calponin homology domain-containing protein 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY204138.1, CN702489.1, CF729493.1, DV661712.1, BQ924416.1 and CA323124.1. Transcript Variant: This variant (2) lacks an internal segment in the 3' region, as compared to variant 1. The resulting isoform (2) is shorter and has a different C-terminus, as compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK088331.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCAG CAGTAGCGGG CCCACTCGCC GCCGGGGGTG AGGAAGCTGC AGCTTCAGTG 
TCCTTGCCAG GGTCTCCTGG TCTACCTGGG AGCCGTAGCG CAGAACGAGC CCTAGAGGAG 
GCTGTGGCCA CCGGGACCCT GAACTTGTCC AACCGGCGTT TGAAGCACTT CCCCCGGGGC 
GCGGCCCGCA GTTACGACTT GTCAGACATC ACCCAGGCTG ACTTGTCTCG GAACCGGTTT 
CCCGAGGTGC CTGAGGCAGC TTGCCAGCTG GTGTCCCTGG AAGGCCTGAG CCTCTACCAC 
AATTGCCTGA AATGCCTGAA CCCAGCCTTG GGGAATCTTA CAGCCCTCAC CTACCTCAAC 
CTCAGCCGGA ACCAGCTGTC GTCGTTGCCA CCCTACATCT GCCAGCTGCC CCTTCGAGTG 
CTTATCATCA GCAACAACAA GTTAGGAGCC CTGCCTCCAG ACATCAGCAC CTTGGGAAGC 
CTGCGGCAGC TTGATGTGAG CAGCAATGAG CTGCAGTCCC TGCCCGTGGA GCTGTGTAGC 
CTCCGTTCCC TGCGGGATCT CAATGTTCGA AGGAACCAGC TCAGTACCCT GCCTGATGAG 
CTGGGAGACC TTCCTCTGGT CCGCCTGGAT TTCTCCTGTA ACCGCATCTC CCGAATCCCC 
GTCTCCTTCT GCCGCCTCAG GCACCTGCAG GTCGTTCTGC TGGATAGCAA CCCCCTACAG 
AGTCCACCTG CCCAGATATG CCTGAAGGGG AAACTTCACA TCTTCAAGTA CCTAACAATG 
GAAGCTGGCC GGAGGGGAGC CGCCCTCGGG GACCTGGTCC CTTCCCGCCC CCCAAGTTTT 
AGTCCTTGCC CTGCCGAAGA TTTATTTCCG GGACGTCGTT ATGATGGTGG CCTGGACTCA 
GGCTTCCACA GCGTTGACAG TGGCAGCAAG AGGTGGTCAG GAAATGAGTC CACAGATGAT 
TTTTCAGAGC TGTCTTTCCG GATCTCGGAG CTGGCTCGTG ATCCCCGGGG GCCTAGACAA 
CCTAGGGAAG ATGGCGCTGG CGATGGAGAC CTGGAGCAGA TTGACTTTAT TGACAGCCAC 
GTTCCTGGGG AAGATGAAGA TCGAAGTGCA GCTGAGGAGC AGCTGCCTTC TGAATTAAGC 
CTTGTAGCAG GGGATGTGGA GAAGCCATCT AGCAGCAGGC GAGAGGAGCC TGCAGGGGAG 
GAGAGGCGGC GCCCAGACAC TTTGCAGTTG TGGCAGGAAC GGGAGCGGAA GCAACAGCAA 
CAGAGTGGGG GATGGGGGTC CCCCAGGAAG GACAGCGTCC TGAAGCGGGG GATCCGAGCT 
GCCGGGGCAG GTGCTTCGGC CCCATCCACA CAGGCCACCT GCAATGGCCC ACCAAAGTCC 
AGCACTACCC AACTGGGAGT TTCAGGGGGG CAGGGAGCTC CCACACCACC CCCCACCTCC 
CAGGACCCCC TTCCTGTATC TGGACCAGTG ACAGCTCCTG TTCCCAGGCC CCTGGGCTCC 
ATTCAGAGAC CAAACAGCTT CCTCTTCCGG TCCTCTTCTC AGAGTGGCTC TAGTCCTTCC 
TCTCCAGAGT CTGTTTTGAG ACCTCGGCCG TTTCCTCAGG AGAAGGAGCT AATATCCCAA 
CTTCGCCAGG TCCTAGAGTC GCGGCTGCAG CAGCCCCTAC CTGAGGACCT GGCAGAAGCT 
CTCGCCAACG GAGTCCTCCT TTGCCAGCTG GCGAACCAGC TGCGGCCCCG CTCTGTACCC 
TTCATTCATG TGCCCTCACC TGCTGTGCCA AAGCTCAGTG CTCTCAAGTC TCGGAAGAAT 
GTCGAGAGTT TCCTAGAAGC CTGTCGGAAA ATGGGTGTGC CTGAGGAGTC CCTGTGCCAG 
CCCCACCACA TCCTGGAAGA GGAAGGGGCC CCTGGAAGGG GCCTCCCTCA CATCGCTGCT 
GTCCTTCACG CACTTCTGGA ACAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162123.1
CDS	85..2034
Translation	MAAAVAGPLAAGGEEAAASVSLPGSPGLPGSRSAERALEEAVATGTLNLSNRRLKHFPRGAARSYDLSDITQADLSRNRFPEVPEAACQLVSLEGLSLYHNCLKCLNPALGNLTALTYLNLSRNQLSSLPPYICQLPLRVLIISNNKLGALPPDISTLGSLRQLDVSSNELQSLPVELCSLRSLRDLNVRRNQLSTLPDELGDLPLVRLDFSCNRISRIPVSFCRLRHLQVVLLDSNPLQSPPAQICLKGKLHIFKYLTMEAGRRGAALGDLVPSRPPSFSPCPAEDLFPGRRYDGGLDSGFHSVDSGSKRWSGNESTDDFSELSFRISELARDPRGPRQPREDGAGDGDLEQIDFIDSHVPGEDEDRSAAEEQLPSELSLVAGDVEKPSSSRREEPAGEERRRPDTLQLWQERERKQQQQSGGWGSPRKDSVLKRGIRAAGAGASAPSTQATCNGPPKSSTTQLGVSGGQGAPTPPPTSQDPLPVSGPVTAPVPRPLGSIQRPNSFLFRSSSQSGSSPSSPESVLRPRPFPQEKELISQLRQVLESRLQQPLPEDLAEALANGVLLCQLANQLRPRSVPFIHVPSPAVPKLSALKSRKNVESFLEACRKMGVPEESLCQPHHILEEEGAPGRGLPHIAAVLHALLEQP

Target ORF information:

RefSeq Version	NM_001168652.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine-rich repeats and calponin homology (CH) domain containing 4 (Lrch4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001168652.1

ORF Insert Sequence:

ATGGCGGCAG CAGTAGCGGG CCCACTCGCC GCCGGGGGTG AGGAAGCTGC AGCTTCAGTG 
TCCTTGCCAG GGTCTCCTGG TCTACCTGGG AGCCGTAGCG CAGAACGAGC CCTAGAGGAG 
GCTGTGGCCA CCGGGACCCT GAACTTGTCC AACCGGCGTT TGAAGCACTT CCCCCGGGGC 
GCGGCCCGCA GTTACGACTT GTCAGACATC ACCCAGGCTG ACTTGTCTCG GAACCGGTTT 
CCCGAGGTGC CTGAGGCAGC TTGCCAGCTG GTGTCCCTGG AAGGCCTGAG CCTCTACCAC 
AATTGCCTGA AATGCCTGAA CCCAGCCTTG GGGAATCTTA CAGCCCTCAC CTACCTCAAC 
CTCAGCCGGA ACCAGCTGTC GTCGTTGCCA CCCTACATCT GCCAGCTGCC CCTTCGAGTG 
CTTATCATCA GCAACAACAA GTTAGGAGCC CTGCCTCCAG ACATCAGCAC CTTGGGAAGC 
CTGCGGCAGC TTGATGTGAG CAGCAATGAG CTGCAGTCCC TGCCCGTGGA GCTGTGTAGC 
CTCCGTTCCC TGCGGGATCT CAATGTTCGA AGGAACCAGC TCAGTACCCT GCCTGATGAG 
CTGGGAGACC TTCCTCTGGT CCGCCTGGAT TTCTCCTGTA ACCGCATCTC CCGAATCCCC 
GTCTCCTTCT GCCGCCTCAG GCACCTGCAG GTCGTTCTGC TGGATAGCAA CCCCCTACAG 
AGTCCACCTG CCCAGATATG CCTGAAGGGG AAACTTCACA TCTTCAAGTA CCTAACAATG 
GAAGCTGGCC GGAGGGGAGC CGCCCTCGGG GACCTGGTCC CTTCCCGCCC CCCAAGTTTT 
AGTCCTTGCC CTGCCGAAGA TTTATTTCCG GGACGTCGTT ATGATGGTGG CCTGGACTCA 
GGCTTCCACA GCGTTGACAG TGGCAGCAAG AGGTGGTCAG GAAATGAGTC CACAGATGAT 
TTTTCAGAGC TGTCTTTCCG GATCTCGGAG CTGGCTCGTG ATCCCCGGGG GCCTAGACAA 
CCTAGGGAAG ATGGCGCTGG CGATGGAGAC CTGGAGCAGA TTGACTTTAT TGACAGCCAC 
GTTCCTGGGG AAGATGAAGA TCGAAGTGCA GCTGAGGAGC AGCTGCCTTC TGAATTAAGC 
CTTGTAGCAG GGGATGTGGA GAAGCCATCT AGCAGCAGGC GAGAGGAGCC TGCAGGGGAG 
GAGAGGCGGC GCCCAGACAC TTTGCAGTTG TGGCAGGAAC GGGAGCGGAA GCAACAGCAA 
CAGAGTGGGG GATGGGGGTC CCCCAGGAAG GACAGCGTCC TGAAGCGGGG GATCCGAGCT 
GCCGGGGCAG GTGCTTCGGC CCCATCCACA CAGGCCACCT GCAATGGCCC ACCAAAGTCC 
AGCACTACCC AACTGGGAGT TTCAGGGGGG CAGGGAGCTC CCACACCACC CCCCACCTCC 
CAGGACCCCC TTCCTGTATC TGGACCAGTG ACAGCTCCTG TTCCCAGGCC CCTGGGCTCC 
ATTCAGAGAC CAAACAGCTT CCTCTTCCGG TCCTCTTCTC AGAGTGGCTC TAGTCCTTCC 
TCTCCAGAGT CTGTTTTGAG ACCTCGGCCG TTTCCTCAGG AGAAGGAGCT AATATCCCAA 
CTTCGCCAGG TCCTAGAGTC GCGGCTGCAG CAGCCCCTAC CTGAGGACCT GGCAGAAGCT 
CTCGCCAACG GAGTCCTCCT TTGCCAGCTG GCGAACCAGC TGCGGCCCCG CTCTGTACCC 
TTCATTCATG TGCCCTCACC TGCTGTGCCA AAGCTCAGTG CTCTCAAGTC TCGGAAGAAT 
GTCGAGAGTT TCCTAGAAGC CTGTCGGAAA ATGGGTGTGC CTGAGGAGTC CCTGTGCCAG 
CCCCACCACA TCCTGGAAGA GGAAGGGGCC CCTGGAAGGG GCCTCCCTCA CATCGCTGCT 
GTCCTTCACG CACTTCTGGA ACAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrch4 ( NM_146164.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666276.1 Mus musculus leucine-rich repeats and calponin homology (CH) domain containing 4 (Lrch4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12279
Clone ID Related Accession (Same CDS sequence)	NM_146164.2
Accession Version	NM_146164.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Mus musculus(house mouse)
Product	leucine-rich repeat and calponin homology domain-containing protein 4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY204138.1, CN702489.1, CF729493.1, DV661712.1, BQ924416.1, DV650548.1, CV783140.1 and AC124730.5. On Dec 12, 2009 this sequence version replaced NM_146164.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.121277.1, BC012525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCAG CAGTAGCGGG CCCACTCGCC GCCGGGGGTG AGGAAGCTGC AGCTTCAGTG 
TCCTTGCCAG GGTCTCCTGG TCTACCTGGG AGCCGTAGCG CAGAACGAGC CCTAGAGGAG 
GCTGTGGCCA CCGGGACCCT GAACTTGTCC AACCGGCGTT TGAAGCACTT CCCCCGGGGC 
GCGGCCCGCA GTTACGACTT GTCAGACATC ACCCAGGCTG ACTTGTCTCG GAACCGGTTT 
CCCGAGGTGC CTGAGGCAGC TTGCCAGCTG GTGTCCCTGG AAGGCCTGAG CCTCTACCAC 
AATTGCCTGA AATGCCTGAA CCCAGCCTTG GGGAATCTTA CAGCCCTCAC CTACCTCAAC 
CTCAGCCGGA ACCAGCTGTC GTCGTTGCCA CCCTACATCT GCCAGCTGCC CCTTCGAGTG 
CTTATCATCA GCAACAACAA GTTAGGAGCC CTGCCTCCAG ACATCAGCAC CTTGGGAAGC 
CTGCGGCAGC TTGATGTGAG CAGCAATGAG CTGCAGTCCC TGCCCGTGGA GCTGTGTAGC 
CTCCGTTCCC TGCGGGATCT CAATGTTCGA AGGAACCAGC TCAGTACCCT GCCTGATGAG 
CTGGGAGACC TTCCTCTGGT CCGCCTGGAT TTCTCCTGTA ACCGCATCTC CCGAATCCCC 
GTCTCCTTCT GCCGCCTCAG GCACCTGCAG GTCGTTCTGC TGGATAGCAA CCCCCTACAG 
AGTCCACCTG CCCAGATATG CCTGAAGGGG AAACTTCACA TCTTCAAGTA CCTAACAATG 
GAAGCTGGCC GGAGGGGAGC CGCCCTCGGG GACCTGGTCC CTTCCCGCCC CCCAAGTTTT 
AGTCCTTGCC CTGCCGAAGA TTTATTTCCG GGACGTCGTT ATGATGGTGG CCTGGACTCA 
GGCTTCCACA GCGTTGACAG TGGCAGCAAG AGGTGGTCAG GAAATGAGTC CACAGATGAT 
TTTTCAGAGC TGTCTTTCCG GATCTCGGAG CTGGCTCGTG ATCCCCGGGG GCCTAGACAA 
CCTAGGGAAG ATGGCGCTGG CGATGGAGAC CTGGAGCAGA TTGACTTTAT TGACAGCCAC 
GTTCCTGGGG AAGATGAAGA TCGAAGTGCA GCTGAGGAGC AGCTGCCTTC TGAATTAAGC 
CTTGTAGCAG GGGATGTGGA GAAGCCATCT AGCAGCAGGC GAGAGGAGCC TGCAGGGGAG 
GAGAGGCGGC GCCCAGACAC TTTGCAGTTG TGGCAGGAAC GGGAGCGGAA GCAACAGCAA 
CAGAGTGGGG GATGGGGGTC CCCCAGGAAG GACAGCGTCC TGAAGCGGGG GATCCGAGCT 
GCCGGGGCAG GTGCTTCGGC CCCATCCACA CAGGCCACCT GCAATGGCCC ACCAAAGTCC 
AGCACTACCC AACTGGGAGT TTCAGGGGGG CAGGGAGCTC CCACACCACC CCCCACCTCC 
CAGGACCCCC TTCCTGTATC TGGACCAGTG ACAGCTCCTG TTCCCAGGCC CCTGGGCTCC 
ATTCAGAGAC CAAACAGCTT CCTCTTCCGG TCCTCTTCTC AGAGTGGCTC TAGTCCTTCC 
TCTCCAGAGT CTGTTTTGAG ACCTCGGCCG TTTCCTCAGG AGAAGGAGCT AATATCCCAA 
CTTCGCCAGG TCCTAGAGTC GCGGCTGCAG CAGCCCCTAC CTGAGGACCT GGCAGAAGCT 
CTCGCCAACG GAGTCCTCCT TTGCCAGCTG GCGAACCAGC TGCGGCCCCG CTCTGTACCC 
TTCATTCATG TGCCCTCACC TGCTGTGCCA AAGCTCAGTG CTCTCAAGTC TCGGAAGAAT 
GTCGAGAGTT TCCTAGAAGC CTGTCGGAAA ATGGGTGTGC CTGAGGCTGA CCTGTGCTCG 
CCCTCGGATC TCCTCCGGGG CACCGCCCAG GGGCTGCAGA CCGTCCTGGA GGCTGTGATC 
CTGGTTGGGG GCAAGGCCCC TCTCCCAGTC CAGCCCTCCT CTGGTCTGGG TGGCTTCCTC 
CTCTTCTACG TGGTCTTCAT GCTGCTGCTC TATGTCGTCT ACACTCGGCT CCTGGGCTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666276.1
CDS	85..2127
Translation	MAAAVAGPLAAGGEEAAASVSLPGSPGLPGSRSAERALEEAVATGTLNLSNRRLKHFPRGAARSYDLSDITQADLSRNRFPEVPEAACQLVSLEGLSLYHNCLKCLNPALGNLTALTYLNLSRNQLSSLPPYICQLPLRVLIISNNKLGALPPDISTLGSLRQLDVSSNELQSLPVELCSLRSLRDLNVRRNQLSTLPDELGDLPLVRLDFSCNRISRIPVSFCRLRHLQVVLLDSNPLQSPPAQICLKGKLHIFKYLTMEAGRRGAALGDLVPSRPPSFSPCPAEDLFPGRRYDGGLDSGFHSVDSGSKRWSGNESTDDFSELSFRISELARDPRGPRQPREDGAGDGDLEQIDFIDSHVPGEDEDRSAAEEQLPSELSLVAGDVEKPSSSRREEPAGEERRRPDTLQLWQERERKQQQQSGGWGSPRKDSVLKRGIRAAGAGASAPSTQATCNGPPKSSTTQLGVSGGQGAPTPPPTSQDPLPVSGPVTAPVPRPLGSIQRPNSFLFRSSSQSGSSPSSPESVLRPRPFPQEKELISQLRQVLESRLQQPLPEDLAEALANGVLLCQLANQLRPRSVPFIHVPSPAVPKLSALKSRKNVESFLEACRKMGVPEADLCSPSDLLRGTAQGLQTVLEAVILVGGKAPLPVQPSSGLGGFLLFYVVFMLLLYVVYTRLLGS

Target ORF information:

RefSeq Version	NM_146164.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine-rich repeats and calponin homology (CH) domain containing 4 (Lrch4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146164.2

ORF Insert Sequence:

ATGGCGGCAG CAGTAGCGGG CCCACTCGCC GCCGGGGGTG AGGAAGCTGC AGCTTCAGTG 
TCCTTGCCAG GGTCTCCTGG TCTACCTGGG AGCCGTAGCG CAGAACGAGC CCTAGAGGAG 
GCTGTGGCCA CCGGGACCCT GAACTTGTCC AACCGGCGTT TGAAGCACTT CCCCCGGGGC 
GCGGCCCGCA GTTACGACTT GTCAGACATC ACCCAGGCTG ACTTGTCTCG GAACCGGTTT 
CCCGAGGTGC CTGAGGCAGC TTGCCAGCTG GTGTCCCTGG AAGGCCTGAG CCTCTACCAC 
AATTGCCTGA AATGCCTGAA CCCAGCCTTG GGGAATCTTA CAGCCCTCAC CTACCTCAAC 
CTCAGCCGGA ACCAGCTGTC GTCGTTGCCA CCCTACATCT GCCAGCTGCC CCTTCGAGTG 
CTTATCATCA GCAACAACAA GTTAGGAGCC CTGCCTCCAG ACATCAGCAC CTTGGGAAGC 
CTGCGGCAGC TTGATGTGAG CAGCAATGAG CTGCAGTCCC TGCCCGTGGA GCTGTGTAGC 
CTCCGTTCCC TGCGGGATCT CAATGTTCGA AGGAACCAGC TCAGTACCCT GCCTGATGAG 
CTGGGAGACC TTCCTCTGGT CCGCCTGGAT TTCTCCTGTA ACCGCATCTC CCGAATCCCC 
GTCTCCTTCT GCCGCCTCAG GCACCTGCAG GTCGTTCTGC TGGATAGCAA CCCCCTACAG 
AGTCCACCTG CCCAGATATG CCTGAAGGGG AAACTTCACA TCTTCAAGTA CCTAACAATG 
GAAGCTGGCC GGAGGGGAGC CGCCCTCGGG GACCTGGTCC CTTCCCGCCC CCCAAGTTTT 
AGTCCTTGCC CTGCCGAAGA TTTATTTCCG GGACGTCGTT ATGATGGTGG CCTGGACTCA 
GGCTTCCACA GCGTTGACAG TGGCAGCAAG AGGTGGTCAG GAAATGAGTC CACAGATGAT 
TTTTCAGAGC TGTCTTTCCG GATCTCGGAG CTGGCTCGTG ATCCCCGGGG GCCTAGACAA 
CCTAGGGAAG ATGGCGCTGG CGATGGAGAC CTGGAGCAGA TTGACTTTAT TGACAGCCAC 
GTTCCTGGGG AAGATGAAGA TCGAAGTGCA GCTGAGGAGC AGCTGCCTTC TGAATTAAGC 
CTTGTAGCAG GGGATGTGGA GAAGCCATCT AGCAGCAGGC GAGAGGAGCC TGCAGGGGAG 
GAGAGGCGGC GCCCAGACAC TTTGCAGTTG TGGCAGGAAC GGGAGCGGAA GCAACAGCAA 
CAGAGTGGGG GATGGGGGTC CCCCAGGAAG GACAGCGTCC TGAAGCGGGG GATCCGAGCT 
GCCGGGGCAG GTGCTTCGGC CCCATCCACA CAGGCCACCT GCAATGGCCC ACCAAAGTCC 
AGCACTACCC AACTGGGAGT TTCAGGGGGG CAGGGAGCTC CCACACCACC CCCCACCTCC 
CAGGACCCCC TTCCTGTATC TGGACCAGTG ACAGCTCCTG TTCCCAGGCC CCTGGGCTCC 
ATTCAGAGAC CAAACAGCTT CCTCTTCCGG TCCTCTTCTC AGAGTGGCTC TAGTCCTTCC 
TCTCCAGAGT CTGTTTTGAG ACCTCGGCCG TTTCCTCAGG AGAAGGAGCT AATATCCCAA 
CTTCGCCAGG TCCTAGAGTC GCGGCTGCAG CAGCCCCTAC CTGAGGACCT GGCAGAAGCT 
CTCGCCAACG GAGTCCTCCT TTGCCAGCTG GCGAACCAGC TGCGGCCCCG CTCTGTACCC 
TTCATTCATG TGCCCTCACC TGCTGTGCCA AAGCTCAGTG CTCTCAAGTC TCGGAAGAAT 
GTCGAGAGTT TCCTAGAAGC CTGTCGGAAA ATGGGTGTGC CTGAGGCTGA CCTGTGCTCG 
CCCTCGGATC TCCTCCGGGG CACCGCCCAG GGGCTGCAGA CCGTCCTGGA GGCTGTGATC 
CTGGTTGGGG GCAAGGCCCC TCTCCCAGTC CAGCCCTCCT CTGGTCTGGG TGGCTTCCTC 
CTCTTCTACG TGGTCTTCAT GCTGCTGCTC TATGTCGTCT ACACTCGGCT CCTGGGCTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Genes & development20(19)2713-27(2006 Oct)
Evsikov AV,Graber JH,Brockman JM,Hampl A,Holbrook AE,Singh P,Eppig JJ,Solter D,Knowles BB

Identification and characterization of SAP25, a novel component of the mSin3 corepressor complex.
Molecular and cellular biology26(4)1386-97(2006 Feb)
Shiio Y,Rose DW,Aur R,Donohoe S,Aebersold R,Eisenman RN

Transcriptome analysis of mouse stem cells and early embryos.
PLoS biology1(3)E74(2003 Dec)
Sharov AA,Piao Y,Matoba R,Dudekula DB,Qian Y,VanBuren V,Falco G,Martin PR,Stagg CA,Bassey UC,Wang Y,Carter MG,Hamatani T,Aiba K,Akutsu H,Sharova L,Tanaka TS,Kimber WL,Yoshikawa T,Jaradat SA,Pantano S,Nagaraja R,Boheler KR,Taub D,Hodes RJ,Longo DL,Schlessinger D,Keller J,Klotz E,Kelsoe G,Umezawa A,Vescovi AL,Rossant J,Kunath T,Hogan BL,Curci A,D'Urso M,Kelso J,Hide W,Ko MS

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Lrch4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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