Fbxw8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fbxw8
Entrez Gene ID	231672
Full Name	F-box and WD-40 domain protein 8
Synonyms	4930438M06Rik,FBW6,FBW8,FBXO29,Fbx29
General protein information	Preferred Names F-box and WD-40 domain protein 8 Names F-box/WD repeat-containing protein 8 F-box and WD-40 domain-containing protein 8 F-box protein FBX29
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
NM_172721.2	NP_766309.2	F-box/WD repeat-containing protein 8

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RNFT2	XP_005636331.1
Bos taurus (cattle)	FBXW8	NP_001192404.1
Gallus gallus (chicken)	FBXW8	XP_415314.2
Danio rerio (zebrafish)	fbxw8	NP_001108050.1
Xenopus tropicalis (tropical clawed frog)	fbxw8	XP_002938134.2
Pan troglodytes (chimpanzee)	FBXW8	XP_509407.2
Homo sapiens (human)	FBXW8	NP_699179.2
	FBXW8	XP_001081979.1
Mus musculus (house mouse)	Fbxw8	NP_766309.2
Rattus norvegicus (Norway rat)	Fbxw8	NP_001100615.1

Gene Ontology
Bibliography

Related articles in PubMed

Serine 347 Phosphorylation by JNKs Negatively Regulates OCT4 Protein Stability in Mouse Embryonic Stem Cells.
Bae KB, Yu DH, Lee KY, Yao K, Ryu J, Lim DY, Zykova TA, Kim MO, Bode AM, Dong Z
Stem cell reports9(6)2050-2064(2017 Dec)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Both ubiquitin ligases FBXW8 and PARK2 are sequestrated into insolubility by ATXN2 PolyQ expansions, but only FBXW8 expression is dysregulated.
Halbach MV, Stehning T, Damrath E, Jendrach M, ?en NE, Ba?ak AN, Auburger G
PloS one10(3)e0121089(2015)

Evidence for a regulatory role of Cullin-RING E3 ubiquitin ligase 7 in insulin signaling.
Scheufele F, Wolf B, Kruse M, Hartmann T, Lempart J, M?hlich S, Pfeiffer AFH, Field LJ, Charron MJ, Pan ZQ, Engelhardt S, Sarikas A
Cellular signalling26(2)233-239(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FBXW8 and PARK2 are sequestrated into insolubility by ATXN2 PolyQ expansions, but only FBXW8 expression is dysregulated
Title: Both ubiquitin ligases FBXW8 and PARK2 are sequestrated into insolubility by ATXN2 PolyQ expansions, but only FBXW8 expression is dysregulated.

mTORC2 negatively feeds back to IRS-1 via control of Fbw8 stability and localization.
Title: mTOR complex 2 regulates proper turnover of insulin receptor substrate-1 via the ubiquitin ligase subunit Fbw8.

our data support the concept that expanded ATXN2 undergoes progressive insolubility and affects PABPC1 by a toxic gain-of-function mechanism with tissue-specific effects, which may be partially alleviated by the induction of FBXW8.
Title: ATXN2-CAG42 sequesters PABPC1 into insolubility and induces FBXW8 in cerebellum of old ataxic knock-in mice.

FBXW8-CUL7 complex plays a significant role in growth control
Title: Disruption of the Fbxw8 gene results in pre- and postnatal growth retardation in mice.

Depletion of FBXW8 caused a significant accumulation of cyclin D1, as well as sequestration of CDK1 in the cytoplasm. This resulted in a severe reduction of cell proliferation.
Title: A critical role for FBXW8 and MAPK in cyclin D1 degradation and cancer cell proliferation.

The following Fbxw8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fbxw8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03951	NM_172721.2 Latest version!	Mus musculus F-box and WD-40 domain protein 8 (Fbxw8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fbxw8 ( NM_172721.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766309.2 Mus musculus F-box and WD-40 domain protein 8 (Fbxw8), mRNA.

Price & Availability↑

CloneID	OMu03951
Clone ID Related Accession (Same CDS sequence)	NM_172721.2
Accession Version	NM_172721.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	F-box/WD repeat-containing protein 8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK053794.1, AK034447.1 and BY468586.1. On Dec 29, 2006 this sequence version replaced NM_172721.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK034447.1, AK134291.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164142 [ECO:0000348] ##Evidence-Data-END##

ATGGACGACC ACAACCTGGA GGAGTTCCGC CGGCACTGGC AGGAGGAGTT GGCGCAGTCA 
CAGGCGCTGA GGCGACGGCG GCGGCTCGAG GCTGGGGAGC GGCGGTCGCC GCGAAGGCCC 
GAGGCGGGAG CGCGAGGCGA GCCGGCCTCG GGCTACCTGG GGCTGGCGCA GGGGCTGCTG 
GAGGGCGCGG GCCGACCCCC GGCGCCCAGG CCTGGCCGGG GAGGCGACAG GAAGGATACG 
TCCAGCCGAT CGCGCTCACC CCCAGACCGC GACGCCACGG AGCCCGAGCC GCTGGTGGAT 
CAGCTCATCC GCGACTTGAA TGAGCTGGAT GACGTGCCCT TCTTTGATGT CCGTCTGCCT 
TACGAACTGG CCATCAATAT ATTCCAGTAT CTGAACAGAA GAGAGCTGGG ACTGTGTGCT 
CAGGTGAGCA AGACATGGAA GGTGATTGCT GAAGACGAGG TGCTGTGGTA CCGGCTGTGC 
CGACAGGAGG GGCACCTTCC CCACAGCAGA TTCTCCGATT ACACCTGCTG GAAGCTCATC 
TTGCAAGAGT GCCTCGCCAA GGAGCACACA TTAAGAGCCA ACTGGAAGAA TCGCAAAGGT 
GCCGTGAGCG AGCTGGAGCA TGTTCCCGAC GCTGTTTTGT GTGACGTGCG TTCTCACGAT 
GGTGTGGTCA TTGCTGGATA CACGTCAGGG GATGTGCGGG TGTGGGACAC CCGCACCTGG 
GACTACGTGG CCCCCTTCCT GGAGTCTGAG TCTGAGGAGG AGGATCCTGG AATGCAGCCA 
TATGTCTCCT TTGTGAGGAT CAACAGCTCG CTGGCAGTGG CGGCTTACGA GGATGGGATC 
CTTAACATTT GGGACCTGAG AACCGGAAGG TTCCCTATCT TTCGTTTTGA GCATGACGCA 
AGAATACAAG CCCTTGCGCT GAGCCAAGAA AAGCCCATTG TTGCCACGGC TTCTGCTTTT 
GACGTTGTGA TGTTGTACCC CAACGAGGAG GGGCATTGGC ATGTGGCCTC GGAGTTTGAA 
GTTCAGAAGC TGGTTGACTA CCTTGAAATA GTTCCGAATA CTGGGAGGTA CCCTGTGGCA 
ATAGCCACAG CCGGGGATCT GGTGTACCTG CTGAAGGCCG ACGACTCAGC CAGAACCCTT 
CATTATGTCT ATGGCCAGCC TGCCACATGT CTGGATGTCT CAGCCAGCCA GGTTGCCTTT 
GGAGTGAAGA GTCTAGGATG GGTGTATGAA GGAAACAAGA TCCTGGTGTA CAGCCTGGAA 
GCAGAGCGCT GCCTCTCGAA GCTGGGCAAT GCACTTGGAG ACTTTACCTG TGTCAACATC 
CGGGATAGCC CTCCCAACCT CATGGTCAGC GGCAACATGG ACAGGAGAGT GAGGATCCAT 
GACCTCCGCA GCGATAAGAT CGCCCTGTCG CTGTCTGCCC ATCAGCTGGG GGTGTCCGCA 
GTCCAGATGG ATGACTGGAA GGTTGTCAGT GGAGGCGAGG AGGGGCTGGT GTCTGTGTGG 
GATTACCGCA TGAACCAGAA GCTGTGGGAA GTGCACTCCA GGCACCCTGT GCGCTATCTC 
TCCTTCAATA GCCACAGCCT CATCACTGCC AACGTGCCCT ACGAGAAGGT GCTGCGAAAC 
TCCGACCTCG ACAACTTTGC CTGTCACAGG AGACATCGTG GCCTGATCCA TGCCTATGAA 
TTTGCTGTGG ACCAGCTGGC CTTTCAGAGC CCCCTTCCTG TCTGCCGCTT ACCCCGTGAC 
ATCATGGCTG GATACAGCTA TGACCTCGCA CTGTCTTTCC CCCATGACAG TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766309.2
CDS	78..1874
Translation	MDDHNLEEFRRHWQEELAQSQALRRRRRLEAGERRSPRRPEAGARGEPASGYLGLAQGLLEGAGRPPAPRPGRGGDRKDTSSRSRSPPDRDATEPEPLVDQLIRDLNELDDVPFFDVRLPYELAINIFQYLNRRELGLCAQVSKTWKVIAEDEVLWYRLCRQEGHLPHSRFSDYTCWKLILQECLAKEHTLRANWKNRKGAVSELEHVPDAVLCDVRSHDGVVIAGYTSGDVRVWDTRTWDYVAPFLESESEEEDPGMQPYVSFVRINSSLAVAAYEDGILNIWDLRTGRFPIFRFEHDARIQALALSQEKPIVATASAFDVVMLYPNEEGHWHVASEFEVQKLVDYLEIVPNTGRYPVAIATAGDLVYLLKADDSARTLHYVYGQPATCLDVSASQVAFGVKSLGWVYEGNKILVYSLEAERCLSKLGNALGDFTCVNIRDSPPNLMVSGNMDRRVRIHDLRSDKIALSLSAHQLGVSAVQMDDWKVVSGGEEGLVSVWDYRMNQKLWEVHSRHPVRYLSFNSHSLITANVPYEKVLRNSDLDNFACHRRHRGLIHAYEFAVDQLAFQSPLPVCRLPRDIMAGYSYDLALSFPHDSI

Target ORF information:

RefSeq Version	NM_172721.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus F-box and WD-40 domain protein 8 (Fbxw8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172721.2

ORF Insert Sequence:

ATGGACGACC ACAACCTGGA GGAGTTCCGC CGGCACTGGC AGGAGGAGTT GGCGCAGTCA 
CAGGCGCTGA GGCGACGGCG GCGGCTCGAG GCTGGGGAGC GGCGGTCGCC GCGAAGGCCC 
GAGGCGGGAG CGCGAGGCGA GCCGGCCTCG GGCTACCTGG GGCTGGCGCA GGGGCTGCTG 
GAGGGCGCGG GCCGACCCCC GGCGCCCAGG CCTGGCCGGG GAGGCGACAG GAAGGATACG 
TCCAGCCGAT CGCGCTCACC CCCAGACCGC GACGCCACGG AGCCCGAGCC GCTGGTGGAT 
CAGCTCATCC GCGACTTGAA TGAGCTGGAT GACGTGCCCT TCTTTGATGT CCGTCTGCCT 
TACGAACTGG CCATCAATAT ATTCCAGTAT CTGAACAGAA GAGAGCTGGG ACTGTGTGCT 
CAGGTGAGCA AGACATGGAA GGTGATTGCT GAAGACGAGG TGCTGTGGTA CCGGCTGTGC 
CGACAGGAGG GGCACCTTCC CCACAGCAGA TTCTCCGATT ACACCTGCTG GAAGCTCATC 
TTGCAAGAGT GCCTCGCCAA GGAGCACACA TTAAGAGCCA ACTGGAAGAA TCGCAAAGGT 
GCCGTGAGCG AGCTGGAGCA TGTTCCCGAC GCTGTTTTGT GTGACGTGCG TTCTCACGAT 
GGTGTGGTCA TTGCTGGATA CACGTCAGGG GATGTGCGGG TGTGGGACAC CCGCACCTGG 
GACTACGTGG CCCCCTTCCT GGAGTCTGAG TCTGAGGAGG AGGATCCTGG AATGCAGCCA 
TATGTCTCCT TTGTGAGGAT CAACAGCTCG CTGGCAGTGG CGGCTTACGA GGATGGGATC 
CTTAACATTT GGGACCTGAG AACCGGAAGG TTCCCTATCT TTCGTTTTGA GCATGACGCA 
AGAATACAAG CCCTTGCGCT GAGCCAAGAA AAGCCCATTG TTGCCACGGC TTCTGCTTTT 
GACGTTGTGA TGTTGTACCC CAACGAGGAG GGGCATTGGC ATGTGGCCTC GGAGTTTGAA 
GTTCAGAAGC TGGTTGACTA CCTTGAAATA GTTCCGAATA CTGGGAGGTA CCCTGTGGCA 
ATAGCCACAG CCGGGGATCT GGTGTACCTG CTGAAGGCCG ACGACTCAGC CAGAACCCTT 
CATTATGTCT ATGGCCAGCC TGCCACATGT CTGGATGTCT CAGCCAGCCA GGTTGCCTTT 
GGAGTGAAGA GTCTAGGATG GGTGTATGAA GGAAACAAGA TCCTGGTGTA CAGCCTGGAA 
GCAGAGCGCT GCCTCTCGAA GCTGGGCAAT GCACTTGGAG ACTTTACCTG TGTCAACATC 
CGGGATAGCC CTCCCAACCT CATGGTCAGC GGCAACATGG ACAGGAGAGT GAGGATCCAT 
GACCTCCGCA GCGATAAGAT CGCCCTGTCG CTGTCTGCCC ATCAGCTGGG GGTGTCCGCA 
GTCCAGATGG ATGACTGGAA GGTTGTCAGT GGAGGCGAGG AGGGGCTGGT GTCTGTGTGG 
GATTACCGCA TGAACCAGAA GCTGTGGGAA GTGCACTCCA GGCACCCTGT GCGCTATCTC 
TCCTTCAATA GCCACAGCCT CATCACTGCC AACGTGCCCT ACGAGAAGGT GCTGCGAAAC 
TCCGACCTCG ACAACTTTGC CTGTCACAGG AGACATCGTG GCCTGATCCA TGCCTATGAA 
TTTGCTGTGG ACCAGCTGGC CTTTCAGAGC CCCCTTCCTG TCTGCCGCTT ACCCCGTGAC 
ATCATGGCTG GATACAGCTA TGACCTCGCA CTGTCTTTCC CCCATGACAG TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Serine 347 Phosphorylation by JNKs Negatively Regulates OCT4 Protein Stability in Mouse Embryonic Stem Cells.
Stem cell reports9(6)2050-2064(2017 Dec)
Bae KB,Yu DH,Lee KY,Yao K,Ryu J,Lim DY,Zykova TA,Kim MO,Bode AM,Dong Z

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Both ubiquitin ligases FBXW8 and PARK2 are sequestrated into insolubility by ATXN2 PolyQ expansions, but only FBXW8 expression is dysregulated.
PloS one10(3)e0121089(2015)
Halbach MV,Stehning T,Damrath E,Jendrach M,?en NE,Ba?ak AN,Auburger G

Evidence for a regulatory role of Cullin-RING E3 ubiquitin ligase 7 in insulin signaling.
Cellular signalling26(2)233-239(2014 Feb)
Scheufele F,Wolf B,Kruse M,Hartmann T,Lempart J,M?hlich S,Pfeiffer AFH,Field LJ,Charron MJ,Pan ZQ,Engelhardt S,Sarikas A

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Fbxw8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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