Ssh1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ssh1
Entrez Gene ID	231637
Full Name	slingshot protein phosphatase 1
Synonyms	AW551225,Gm1394,Gm1395,SSH-1,SSH-1L
General protein information	Preferred Names slingshot protein phosphatase 1 Names protein phosphatase Slingshot homolog 1 SSH-like protein 1 slingshot 1L slingshot homolog 1 slingshot-like 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
NM_198109.4	NP_932777.2	protein phosphatase Slingshot homolog 1
XM_030254423.1	XP_030110283.1	protein phosphatase Slingshot homolog 1 isoform X2
NM_001363469.1	NP_001350398.1	protein phosphatase Slingshot homolog 1 isoform 2
XM_011248193.2	XP_011246495.1	protein phosphatase Slingshot homolog 1 isoform X1
NM_198109.5	NP_932777.2	protein phosphatase Slingshot homolog 1 isoform 1
XM_006530288.3	XP_006530351.1	protein phosphatase Slingshot homolog 1 isoform X2
XM_011248193.3	XP_011246495.1	protein phosphatase Slingshot homolog 1 isoform X1
XM_006530288.4	XP_006530351.1	protein phosphatase Slingshot homolog 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SSH1	XP_522524.3
	SSH1	XP_001096246.2
Mus musculus (house mouse)	Ssh1	NP_932777.2
Rattus norvegicus (Norway rat)	Ssh1	XP_006249574.1
Danio rerio (zebrafish)	ssh1b	XP_695867.2
Bos taurus (cattle)	SSH1	XP_002694647.2
Xenopus tropicalis (tropical clawed frog)	ssh1	XP_004910677.1
Homo sapiens (human)	SSH1	NP_061857.3
Gallus gallus (chicken)	SSH1	XP_004945673.1
Danio rerio (zebrafish)	LOC101885659	XP_005165255.1
Canis lupus familiaris (dog)	SSH1	XP_851712.1

Gene Ontology
Bibliography

Related articles in PubMed

Caspase-11 and caspase-1 differentially modulate actin polymerization via RhoA and Slingshot proteins to promote bacterial clearance.
Caution K, Gavrilin MA, Tazi M, Kanneganti A, Layman D, Hoque S, Krause K, Amer AO
Scientific reports518479(2015 Dec)

High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes.
Brommage R, Liu J, Hansen GM, Kirkpatrick LL, Potter DG, Sands AT, Zambrowicz B, Powell DR, Vogel P
Bone research214034(2014)

PLC?, PKD1, and SSH1L transduce RhoA signaling to protect mitochondria from oxidative stress in the heart.
Xiang SY, Ouyang K, Yung BS, Miyamoto S, Smrcka AV, Chen J, Heller Brown J
Science signaling6(306)ra108(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Caspase-11 targets cofilin via the RhoA GTPase, whereas caspase-1 engages the Slingshot phosphatase
Title: Caspase-11 and caspase-1 differentially modulate actin polymerization via RhoA and Slingshot proteins to promote bacterial clearance.

SSH1L knockdown, like S1P treatment, increased cardiomyocyte survival and preserved mitochondrial integrity after oxidative stress
Title: PLC?, PKD1, and SSH1L transduce RhoA signaling to protect mitochondria from oxidative stress in the heart.

EphA signaling promotes actin-based dendritic spine remodeling through slingshot 1.
Title: EphA signaling promotes actin-based dendritic spine remodeling through slingshot phosphatase.

PDGF activates SSH1L in VSMC by a mechanism that involves Nox1-mediated oxidation of 14-3-3 and Ser-834 SSH1L auto-dephosphorylation.
Title: Platelet-derived growth factor (PDGF) regulates Slingshot phosphatase activity via Nox1-dependent auto-dephosphorylation of serine 834 in vascular smooth muscle cells.

Knockdown of SSH1 expression by RNA interference significantly suppressed stress fiber formation in C2C12 myoblast cells, indicating a role for SSH1 in stress fiber formation or stabilization in cells.
Title: Actin filaments-stabilizing and -bundling activities of cofilin-phosphatase Slingshot-1.

The following Ssh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ssh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu35870	XM_006530288.4 Latest version!	Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu35870	XM_030254423.1 Latest version!	Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu84386	NM_001363469.1 Latest version!	Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu73571	XM_011248193.3 Latest version!	Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu08870	NM_198109.5 Latest version!	Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu08870	NM_198109.4	Mus musculus slingshot protein phosphatase 1 (Ssh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu73571	XM_011248193.2	Mus musculus slingshot homolog 1 (Drosophila) (Ssh1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu35870	XM_006530288.3	Mus musculus slingshot homolog 1 (Drosophila) (Ssh1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ssh1 ( XM_006530288.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530351.1 Mus musculus slingshot homolog 1 (Drosophila) (Ssh1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu35870
Clone ID Related Accession (Same CDS sequence)	XM_030254423.1 , XM_006530288.3 , XM_006530288.4
Accession Version	XM_006530288.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein phosphatase Slingshot homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530288.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAAG GAGCTGCCCT CTTCCTACAG CAGGGAAACA GCCCACAGGG CCAGCGGAGT 
CTTCAGCACC CTCACAAGCA TGCAGGTGAT CTGCCTCAGC ACCTGCAAGT GATGATCAAC 
CTCCTGCGTT GTGAAGACAG AATCAAGCTG GCCGTGCGCC TAGAGAGTGT CTGGACCGAC 
CGTGTCCGCT ACATGGTCGT GGTATACACC AGCGGGCGCC AGGACACCGA GGAGAATATT 
CTGCTGGGAG TTGACTTTTC CAGTAAGGAG AGCAAAAGCT GCACGATCGG AATGGTTCTT 
CGACTCTGGA GCGACACCAA GATTCACCTC GATGGGGACG GCGGGTTCAG TGTGAGCACA 
GCGGGCAGGA TGCACATATT CAAGCCAGTG TCTGTCCAGG CCATGTGGTC TGCCCTGCAA 
GTGCTTCACA AGGCCTGCGA AGTGGCCCGG AGGCATAACT ACTTCCCAGG AGGAGTGGCG 
CTCATCTGGG CCACCTACTA TGAGAGCTGC ATCAGCTCGG AGCAGAGCTG CATCAATGAG 
TGGAATGCCA TGCAGGACCT GGAGTCCACG CGGCCCGACT CCCCCGCGCT GTTTGTGGAC 
AAGCCAACCG AGGGCGAAAG AACTGAGCGC CTCATTAAAG CCAAACTCCG GAGCATCATG 
ATGAGCCAGG ACCTTGAAAA TGTGACTTCT AAGGAAATCC GAAATGAGCT GGAGAAGCAA 
ATGAACTGCA ACCTGAAGGA GTTCAAGGAA TTCATCGATA ACGAGATGCT GCTCATCCTG 
GGCCAGATGG ACAAGCCCTC CCTCATCTTT GACCATCTTT ATCTTGGCTC CGAGTGGAAT 
GCATCCAATC TGGAGGAGCT GCAGGGCTCA GGAGTTGACT ACATTTTAAA TGTCACTAGA 
GAAATAGACA ATTTTTTCCC CGGCTTGTTT GCGTACCATA ACATCCGCGT GTACGATGAG 
GAGACCACAG ACCTTCTTGC CCACTGGAAT GAAGCTTATC ATTTCATAAA CAAAGCGAAA 
AGGAATCATT CCAAGTGCCT GGTCCATTGC AAGATGGGCG TCAGCCGATC TGCGTCCACA 
GTCATTGCCT ACGCCATGAA GGAGTTCGGC TGGCCCCTGG AGAAAGCGTA TAACTACGTG 
AAGCAGAAAC GTAGTATCAC TCGGCCCAAC GCAGGCTTTA TGAGGCAGCT GTCTGAGTAC 
GAAGGCATCC TGGATGCAAG CAAACAGCGG CATAACAAAC TATGGCGCCA GCAGCCCACA 
GATGACACCA TCGCAGAGCC CAGTGAGTTC TTGCCAGAGA CCTTGGACGG AGCCCTGGAC 
GCTCAGCTGC CCTGTTTGGA TGATACCACC CACCCTGGGC TCCCGAGAAG TCTGGCCCCA 
GGAGGACCCG CTCTCCCCTG TTGTTTTCGA AGACTCTCGG ACCCCCTCCT CCTTCCCCAC 
CATGATGAAA CGGGTGGCCT GGTCCACTTA GAGGATCTCG AGAAGGATGC TCTGTTAGAG 
GAGGAGGAGT CTCAGCCAGT GGAGGTGCAC AAGCTGGTTC AGCATCCCCA GGAAGGTGCC 
AGGCTGTGTG AAAAGGACGT AAAGAGGAAA CTGGAGTTTG GGAACTCCAA ACCCCGCAGT 
GACTCCTTGC CTCAGGTGGA GGAGCTGGAG AAGGACGGTA GCCCAAGAAC GGGGAGGTGG 
AGGCGGGCCT CCACCCAGCT CGATAGAAGC TTGCTCGACC AGGAAAACCT AAATAACAAC 
AACAGCAAGA GGAGCTGTCC CGATGACTTG GAGCGCGATG CCATGTTTGG AATTCTCAGC 
AAAGTGAAGC CTCCCTACAC ATCCTGTGCC GACTGCATGT ACCCCACAGC TGGTGGGACT 
CCCGAGGCCT ACATGGAGCG ACACGAAGAC CCCAGCTCCT CTGCTATCTG CACCCAGCCA 
ACCTTCCTCC CCCATGTCAC GTCTTCCCCA ATGGCCCACG CGAGCAGCAG GTCCCGAGCT 
CCGGAGAGGC CGGCCTCGGG TCCAGCCAAC ACCTCTCCAT TCCTACTACC AGCAGGCTCC 
AGGAAGCCAG ATGTCAGTGG CTCTGGAGCC GGGGCTGCCC CGGAACCACC AGCAAGCCTT 
CTAGAGCCTT CCAGAGAGAC CTCAAAAGCC CTTCCAAAAT CTCTCCAGTT GAAGAATCCT 
CACTGTGACA AGAACGCTGC CAATATGGAG GTGTCGGCGA AGGAAGAACC GTCGCCTAAG 
AAAGATCCCA AGCCGGCTAA GGACCTGAGG CTTCTGTTCA GTAACGAGGC GGAGAAGCCC 
ACCACCAACA GCTACCTGAT GCAGCATCAG GAGTCCATCA TCCAACTGCA GAAGGCAGGC 
CTGGTCCGAA AGCACACCAA AGAGCTGGAG AGGTTGAAGA GCCTGCCTTC AGACTCACCA 
GCTGCCTGCA GGGACAGCGC CACCTGCAGG CTGGAGGCCA GCATCCCGGA GGAGGGTAGC 
CAGGAGCCCG CACACCCAGC CCTGTGCAGC CAAGCTGGGT CGGAAGAACA GCCTGTAGGG 
GGAACCTTGC AGAAGAGCCC CACGTCTACC CTCCCCCGTT TAGATCACAC CAGTAACTTC 
TCGAAGGACT TCCTGAAGAC CGTGTGTTAC ACCCCCACCT CCTCCTCCAT CAGCTCCAAC 
CTGACCCGGA GCTCCAGCAG CGACAGCATC CACAGCGTCC GAGGGAAGCC TGGGCTGGTG 
AAGCAGCGGG CGCAGGAGAT CGAGACGCGG CTGCGCCTGG CAGGCCTCAC CGTGTCGTCC 
CCGCTGAAAA GGTCCCATTC CCTTGCCAAG CTGGGAAGTC TCAACTTCTC CACGGAGGAC 
CTGTCCAGCG AGGCTGACAC ATCCACCATC GCTGACTCGC AGGACGCCAA GTGTGGTCTC 
TCCTCTTCCT TCTTGCCCGA ACCCCAGTCT GCGCCAAGGG ACCCCGCTGC AACCTCTAAA 
TCATCAGGGA AATCTGCCCC AGAACACTTG AAAAGCCCGT CGAGGGTAAA CAAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530351.1
CDS	323..3322
Translation	MVKGAALFLQQGNSPQGQRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESVWTDRVRYMVVVYTSGRQDTEENILLGVDFSSKESKSCTIGMVLRLWSDTKIHLDGDGGFSVSTAGRMHIFKPVSVQAMWSALQVLHKACEVARRHNYFPGGVALIWATYYESCISSEQSCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEIRNELEKQMNCNLKEFKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYILNVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAMKEFGWPLEKAYNYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQPTDDTIAEPSEFLPETLDGALDAQLPCLDDTTHPGLPRSLAPGGPALPCCFRRLSDPLLLPHHDETGGLVHLEDLEKDALLEEEESQPVEVHKLVQHPQEGARLCEKDVKRKLEFGNSKPRSDSLPQVEELEKDGSPRTGRWRRASTQLDRSLLDQENLNNNNSKRSCPDDLERDAMFGILSKVKPPYTSCADCMYPTAGGTPEAYMERHEDPSSSAICTQPTFLPHVTSSPMAHASSRSRAPERPASGPANTSPFLLPAGSRKPDVSGSGAGAAPEPPASLLEPSRETSKALPKSLQLKNPHCDKNAANMEVSAKEEPSPKKDPKPAKDLRLLFSNEAEKPTTNSYLMQHQESIIQLQKAGLVRKHTKELERLKSLPSDSPAACRDSATCRLEASIPEEGSQEPAHPALCSQAGSEEQPVGGTLQKSPTSTLPRLDHTSNFSKDFLKTVCYTPTSSSISSNLTRSSSSDSIHSVRGKPGLVKQRAQEIETRLRLAGLTVSSPLKRSHSLAKLGSLNFSTEDLSSEADTSTIADSQDAKCGLSSSFLPEPQSAPRDPAATSKSSGKSAPEHLKSPSRVNKS

Target ORF information:

RefSeq Version	XM_006530288.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus slingshot homolog 1 (Drosophila) (Ssh1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530288.3

ORF Insert Sequence:

ATGGTGAAAG GAGCTGCCCT CTTCCTACAG CAGGGAAACA GCCCACAGGG CCAGCGGAGT 
CTTCAGCACC CTCACAAGCA TGCAGGTGAT CTGCCTCAGC ACCTGCAAGT GATGATCAAC 
CTCCTGCGTT GTGAAGACAG AATCAAGCTG GCCGTGCGCC TAGAGAGTGT CTGGACCGAC 
CGTGTCCGCT ACATGGTCGT GGTATACACC AGCGGGCGCC AGGACACCGA GGAGAATATT 
CTGCTGGGAG TTGACTTTTC CAGTAAGGAG AGCAAAAGCT GCACGATCGG AATGGTTCTT 
CGACTCTGGA GCGACACCAA GATTCACCTC GATGGGGACG GCGGGTTCAG TGTGAGCACA 
GCGGGCAGGA TGCACATATT CAAGCCAGTG TCTGTCCAGG CCATGTGGTC TGCCCTGCAA 
GTGCTTCACA AGGCCTGCGA AGTGGCCCGG AGGCATAACT ACTTCCCAGG AGGAGTGGCG 
CTCATCTGGG CCACCTACTA TGAGAGCTGC ATCAGCTCGG AGCAGAGCTG CATCAATGAG 
TGGAATGCCA TGCAGGACCT GGAGTCCACG CGGCCCGACT CCCCCGCGCT GTTTGTGGAC 
AAGCCAACCG AGGGCGAAAG AACTGAGCGC CTCATTAAAG CCAAACTCCG GAGCATCATG 
ATGAGCCAGG ACCTTGAAAA TGTGACTTCT AAGGAAATCC GAAATGAGCT GGAGAAGCAA 
ATGAACTGCA ACCTGAAGGA GTTCAAGGAA TTCATCGATA ACGAGATGCT GCTCATCCTG 
GGCCAGATGG ACAAGCCCTC CCTCATCTTT GACCATCTTT ATCTTGGCTC CGAGTGGAAT 
GCATCCAATC TGGAGGAGCT GCAGGGCTCA GGAGTTGACT ACATTTTAAA TGTCACTAGA 
GAAATAGACA ATTTTTTCCC CGGCTTGTTT GCGTACCATA ACATCCGCGT GTACGATGAG 
GAGACCACAG ACCTTCTTGC CCACTGGAAT GAAGCTTATC ATTTCATAAA CAAAGCGAAA 
AGGAATCATT CCAAGTGCCT GGTCCATTGC AAGATGGGCG TCAGCCGATC TGCGTCCACA 
GTCATTGCCT ACGCCATGAA GGAGTTCGGC TGGCCCCTGG AGAAAGCGTA TAACTACGTG 
AAGCAGAAAC GTAGTATCAC TCGGCCCAAC GCAGGCTTTA TGAGGCAGCT GTCTGAGTAC 
GAAGGCATCC TGGATGCAAG CAAACAGCGG CATAACAAAC TATGGCGCCA GCAGCCCACA 
GATGACACCA TCGCAGAGCC CAGTGAGTTC TTGCCAGAGA CCTTGGACGG AGCCCTGGAC 
GCTCAGCTGC CCTGTTTGGA TGATACCACC CACCCTGGGC TCCCGAGAAG TCTGGCCCCA 
GGAGGACCCG CTCTCCCCTG TTGTTTTCGA AGACTCTCGG ACCCCCTCCT CCTTCCCCAC 
CATGATGAAA CGGGTGGCCT GGTCCACTTA GAGGATCTCG AGAAGGATGC TCTGTTAGAG 
GAGGAGGAGT CTCAGCCAGT GGAGGTGCAC AAGCTGGTTC AGCATCCCCA GGAAGGTGCC 
AGGCTGTGTG AAAAGGACGT AAAGAGGAAA CTGGAGTTTG GGAACTCCAA ACCCCGCAGT 
GACTCCTTGC CTCAGGTGGA GGAGCTGGAG AAGGACGGTA GCCCAAGAAC GGGGAGGTGG 
AGGCGGGCCT CCACCCAGCT CGATAGAAGC TTGCTCGACC AGGAAAACCT AAATAACAAC 
AACAGCAAGA GGAGCTGTCC CGATGACTTG GAGCGCGATG CCATGTTTGG AATTCTCAGC 
AAAGTGAAGC CTCCCTACAC ATCCTGTGCC GACTGCATGT ACCCCACAGC TGGTGGGACT 
CCCGAGGCCT ACATGGAGCG ACACGAAGAC CCCAGCTCCT CTGCTATCTG CACCCAGCCA 
ACCTTCCTCC CCCATGTCAC GTCTTCCCCA ATGGCCCACG CGAGCAGCAG GTCCCGAGCT 
CCGGAGAGGC CGGCCTCGGG TCCAGCCAAC ACCTCTCCAT TCCTACTACC AGCAGGCTCC 
AGGAAGCCAG ATGTCAGTGG CTCTGGAGCC GGGGCTGCCC CGGAACCACC AGCAAGCCTT 
CTAGAGCCTT CCAGAGAGAC CTCAAAAGCC CTTCCAAAAT CTCTCCAGTT GAAGAATCCT 
CACTGTGACA AGAACGCTGC CAATATGGAG GTGTCGGCGA AGGAAGAACC GTCGCCTAAG 
AAAGATCCCA AGCCGGCTAA GGACCTGAGG CTTCTGTTCA GTAACGAGGC GGAGAAGCCC 
ACCACCAACA GCTACCTGAT GCAGCATCAG GAGTCCATCA TCCAACTGCA GAAGGCAGGC 
CTGGTCCGAA AGCACACCAA AGAGCTGGAG AGGTTGAAGA GCCTGCCTTC AGACTCACCA 
GCTGCCTGCA GGGACAGCGC CACCTGCAGG CTGGAGGCCA GCATCCCGGA GGAGGGTAGC 
CAGGAGCCCG CACACCCAGC CCTGTGCAGC CAAGCTGGGT CGGAAGAACA GCCTGTAGGG 
GGAACCTTGC AGAAGAGCCC CACGTCTACC CTCCCCCGTT TAGATCACAC CAGTAACTTC 
TCGAAGGACT TCCTGAAGAC CGTGTGTTAC ACCCCCACCT CCTCCTCCAT CAGCTCCAAC 
CTGACCCGGA GCTCCAGCAG CGACAGCATC CACAGCGTCC GAGGGAAGCC TGGGCTGGTG 
AAGCAGCGGG CGCAGGAGAT CGAGACGCGG CTGCGCCTGG CAGGCCTCAC CGTGTCGTCC 
CCGCTGAAAA GGTCCCATTC CCTTGCCAAG CTGGGAAGTC TCAACTTCTC CACGGAGGAC 
CTGTCCAGCG AGGCTGACAC ATCCACCATC GCTGACTCGC AGGACGCCAA GTGTGGTCTC 
TCCTCTTCCT TCTTGCCCGA ACCCCAGTCT GCGCCAAGGG ACCCCGCTGC AACCTCTAAA 
TCATCAGGGA AATCTGCCCC AGAACACTTG AAAAGCCCGT CGAGGGTAAA CAAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ssh1 ( XM_030254423.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110283.1 Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu35870
Clone ID Related Accession (Same CDS sequence)	XM_030254423.1 , XM_006530288.3 , XM_006530288.4
Accession Version	XM_030254423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein phosphatase Slingshot homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAAG GAGCTGCCCT CTTCCTACAG CAGGGAAACA GCCCACAGGG CCAGCGGAGT 
CTTCAGCACC CTCACAAGCA TGCAGGTGAT CTGCCTCAGC ACCTGCAAGT GATGATCAAC 
CTCCTGCGTT GTGAAGACAG AATCAAGCTG GCCGTGCGCC TAGAGAGTGT CTGGACCGAC 
CGTGTCCGCT ACATGGTCGT GGTATACACC AGCGGGCGCC AGGACACCGA GGAGAATATT 
CTGCTGGGAG TTGACTTTTC CAGTAAGGAG AGCAAAAGCT GCACGATCGG AATGGTTCTT 
CGACTCTGGA GCGACACCAA GATTCACCTC GATGGGGACG GCGGGTTCAG TGTGAGCACA 
GCGGGCAGGA TGCACATATT CAAGCCAGTG TCTGTCCAGG CCATGTGGTC TGCCCTGCAA 
GTGCTTCACA AGGCCTGCGA AGTGGCCCGG AGGCATAACT ACTTCCCAGG AGGAGTGGCG 
CTCATCTGGG CCACCTACTA TGAGAGCTGC ATCAGCTCGG AGCAGAGCTG CATCAATGAG 
TGGAATGCCA TGCAGGACCT GGAGTCCACG CGGCCCGACT CCCCCGCGCT GTTTGTGGAC 
AAGCCAACCG AGGGCGAAAG AACTGAGCGC CTCATTAAAG CCAAACTCCG GAGCATCATG 
ATGAGCCAGG ACCTTGAAAA TGTGACTTCT AAGGAAATCC GAAATGAGCT GGAGAAGCAA 
ATGAACTGCA ACCTGAAGGA GTTCAAGGAA TTCATCGATA ACGAGATGCT GCTCATCCTG 
GGCCAGATGG ACAAGCCCTC CCTCATCTTT GACCATCTTT ATCTTGGCTC CGAGTGGAAT 
GCATCCAATC TGGAGGAGCT GCAGGGCTCA GGAGTTGACT ACATTTTAAA TGTCACTAGA 
GAAATAGACA ATTTTTTCCC CGGCTTGTTT GCGTACCATA ACATCCGCGT GTACGATGAG 
GAGACCACAG ACCTTCTTGC CCACTGGAAT GAAGCTTATC ATTTCATAAA CAAAGCGAAA 
AGGAATCATT CCAAGTGCCT GGTCCATTGC AAGATGGGCG TCAGCCGATC TGCGTCCACA 
GTCATTGCCT ACGCCATGAA GGAGTTCGGC TGGCCCCTGG AGAAAGCGTA TAACTACGTG 
AAGCAGAAAC GTAGTATCAC TCGGCCCAAC GCAGGCTTTA TGAGGCAGCT GTCTGAGTAC 
GAAGGCATCC TGGATGCAAG CAAACAGCGG CATAACAAAC TATGGCGCCA GCAGCCCACA 
GATGACACCA TCGCAGAGCC CAGTGAGTTC TTGCCAGAGA CCTTGGACGG AGCCCTGGAC 
GCTCAGCTGC CCTGTTTGGA TGATACCACC CACCCTGGGC TCCCGAGAAG TCTGGCCCCA 
GGAGGACCCG CTCTCCCCTG TTGTTTTCGA AGACTCTCGG ACCCCCTCCT CCTTCCCCAC 
CATGATGAAA CGGGTGGCCT GGTCCACTTA GAGGATCTCG AGAAGGATGC TCTGTTAGAG 
GAGGAGGAGT CTCAGCCAGT GGAGGTGCAC AAGCTGGTTC AGCATCCCCA GGAAGGTGCC 
AGGCTGTGTG AAAAGGACGT AAAGAGGAAA CTGGAGTTTG GGAACTCCAA ACCCCGCAGT 
GACTCCTTGC CTCAGGTGGA GGAGCTGGAG AAGGACGGTA GCCCAAGAAC GGGGAGGTGG 
AGGCGGGCCT CCACCCAGCT CGATAGAAGC TTGCTCGACC AGGAAAACCT AAATAACAAC 
AACAGCAAGA GGAGCTGTCC CGATGACTTG GAGCGCGATG CCATGTTTGG AATTCTCAGC 
AAAGTGAAGC CTCCCTACAC ATCCTGTGCC GACTGCATGT ACCCCACAGC TGGTGGGACT 
CCCGAGGCCT ACATGGAGCG ACACGAAGAC CCCAGCTCCT CTGCTATCTG CACCCAGCCA 
ACCTTCCTCC CCCATGTCAC GTCTTCCCCA ATGGCCCACG CGAGCAGCAG GTCCCGAGCT 
CCGGAGAGGC CGGCCTCGGG TCCAGCCAAC ACCTCTCCAT TCCTACTACC AGCAGGCTCC 
AGGAAGCCAG ATGTCAGTGG CTCTGGAGCC GGGGCTGCCC CGGAACCACC AGCAAGCCTT 
CTAGAGCCTT CCAGAGAGAC CTCAAAAGCC CTTCCAAAAT CTCTCCAGTT GAAGAATCCT 
CACTGTGACA AGAACGCTGC CAATATGGAG GTGTCGGCGA AGGAAGAACC GTCGCCTAAG 
AAAGATCCCA AGCCGGCTAA GGACCTGAGG CTTCTGTTCA GTAACGAGGC GGAGAAGCCC 
ACCACCAACA GCTACCTGAT GCAGCATCAG GAGTCCATCA TCCAACTGCA GAAGGCAGGC 
CTGGTCCGAA AGCACACCAA AGAGCTGGAG AGGTTGAAGA GCCTGCCTTC AGACTCACCA 
GCTGCCTGCA GGGACAGCGC CACCTGCAGG CTGGAGGCCA GCATCCCGGA GGAGGGTAGC 
CAGGAGCCCG CACACCCAGC CCTGTGCAGC CAAGCTGGGT CGGAAGAACA GCCTGTAGGG 
GGAACCTTGC AGAAGAGCCC CACGTCTACC CTCCCCCGTT TAGATCACAC CAGTAACTTC 
TCGAAGGACT TCCTGAAGAC CGTGTGTTAC ACCCCCACCT CCTCCTCCAT CAGCTCCAAC 
CTGACCCGGA GCTCCAGCAG CGACAGCATC CACAGCGTCC GAGGGAAGCC TGGGCTGGTG 
AAGCAGCGGG CGCAGGAGAT CGAGACGCGG CTGCGCCTGG CAGGCCTCAC CGTGTCGTCC 
CCGCTGAAAA GGTCCCATTC CCTTGCCAAG CTGGGAAGTC TCAACTTCTC CACGGAGGAC 
CTGTCCAGCG AGGCTGACAC ATCCACCATC GCTGACTCGC AGGACGCCAA GTGTGGTCTC 
TCCTCTTCCT TCTTGCCCGA ACCCCAGTCT GCGCCAAGGG ACCCCGCTGC AACCTCTAAA 
TCATCAGGGA AATCTGCCCC AGAACACTTG AAAAGCCCGT CGAGGGTAAA CAAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110283.1
CDS	241..3240
Translation	MVKGAALFLQQGNSPQGQRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESVWTDRVRYMVVVYTSGRQDTEENILLGVDFSSKESKSCTIGMVLRLWSDTKIHLDGDGGFSVSTAGRMHIFKPVSVQAMWSALQVLHKACEVARRHNYFPGGVALIWATYYESCISSEQSCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEIRNELEKQMNCNLKEFKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYILNVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAMKEFGWPLEKAYNYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQPTDDTIAEPSEFLPETLDGALDAQLPCLDDTTHPGLPRSLAPGGPALPCCFRRLSDPLLLPHHDETGGLVHLEDLEKDALLEEEESQPVEVHKLVQHPQEGARLCEKDVKRKLEFGNSKPRSDSLPQVEELEKDGSPRTGRWRRASTQLDRSLLDQENLNNNNSKRSCPDDLERDAMFGILSKVKPPYTSCADCMYPTAGGTPEAYMERHEDPSSSAICTQPTFLPHVTSSPMAHASSRSRAPERPASGPANTSPFLLPAGSRKPDVSGSGAGAAPEPPASLLEPSRETSKALPKSLQLKNPHCDKNAANMEVSAKEEPSPKKDPKPAKDLRLLFSNEAEKPTTNSYLMQHQESIIQLQKAGLVRKHTKELERLKSLPSDSPAACRDSATCRLEASIPEEGSQEPAHPALCSQAGSEEQPVGGTLQKSPTSTLPRLDHTSNFSKDFLKTVCYTPTSSSISSNLTRSSSSDSIHSVRGKPGLVKQRAQEIETRLRLAGLTVSSPLKRSHSLAKLGSLNFSTEDLSSEADTSTIADSQDAKCGLSSSFLPEPQSAPRDPAATSKSSGKSAPEHLKSPSRVNKS

Target ORF information:

RefSeq Version	XM_030254423.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254423.1

ORF Insert Sequence:

ATGGTGAAAG GAGCTGCCCT CTTCCTACAG CAGGGAAACA GCCCACAGGG CCAGCGGAGT 
CTTCAGCACC CTCACAAGCA TGCAGGTGAT CTGCCTCAGC ACCTGCAAGT GATGATCAAC 
CTCCTGCGTT GTGAAGACAG AATCAAGCTG GCCGTGCGCC TAGAGAGTGT CTGGACCGAC 
CGTGTCCGCT ACATGGTCGT GGTATACACC AGCGGGCGCC AGGACACCGA GGAGAATATT 
CTGCTGGGAG TTGACTTTTC CAGTAAGGAG AGCAAAAGCT GCACGATCGG AATGGTTCTT 
CGACTCTGGA GCGACACCAA GATTCACCTC GATGGGGACG GCGGGTTCAG TGTGAGCACA 
GCGGGCAGGA TGCACATATT CAAGCCAGTG TCTGTCCAGG CCATGTGGTC TGCCCTGCAA 
GTGCTTCACA AGGCCTGCGA AGTGGCCCGG AGGCATAACT ACTTCCCAGG AGGAGTGGCG 
CTCATCTGGG CCACCTACTA TGAGAGCTGC ATCAGCTCGG AGCAGAGCTG CATCAATGAG 
TGGAATGCCA TGCAGGACCT GGAGTCCACG CGGCCCGACT CCCCCGCGCT GTTTGTGGAC 
AAGCCAACCG AGGGCGAAAG AACTGAGCGC CTCATTAAAG CCAAACTCCG GAGCATCATG 
ATGAGCCAGG ACCTTGAAAA TGTGACTTCT AAGGAAATCC GAAATGAGCT GGAGAAGCAA 
ATGAACTGCA ACCTGAAGGA GTTCAAGGAA TTCATCGATA ACGAGATGCT GCTCATCCTG 
GGCCAGATGG ACAAGCCCTC CCTCATCTTT GACCATCTTT ATCTTGGCTC CGAGTGGAAT 
GCATCCAATC TGGAGGAGCT GCAGGGCTCA GGAGTTGACT ACATTTTAAA TGTCACTAGA 
GAAATAGACA ATTTTTTCCC CGGCTTGTTT GCGTACCATA ACATCCGCGT GTACGATGAG 
GAGACCACAG ACCTTCTTGC CCACTGGAAT GAAGCTTATC ATTTCATAAA CAAAGCGAAA 
AGGAATCATT CCAAGTGCCT GGTCCATTGC AAGATGGGCG TCAGCCGATC TGCGTCCACA 
GTCATTGCCT ACGCCATGAA GGAGTTCGGC TGGCCCCTGG AGAAAGCGTA TAACTACGTG 
AAGCAGAAAC GTAGTATCAC TCGGCCCAAC GCAGGCTTTA TGAGGCAGCT GTCTGAGTAC 
GAAGGCATCC TGGATGCAAG CAAACAGCGG CATAACAAAC TATGGCGCCA GCAGCCCACA 
GATGACACCA TCGCAGAGCC CAGTGAGTTC TTGCCAGAGA CCTTGGACGG AGCCCTGGAC 
GCTCAGCTGC CCTGTTTGGA TGATACCACC CACCCTGGGC TCCCGAGAAG TCTGGCCCCA 
GGAGGACCCG CTCTCCCCTG TTGTTTTCGA AGACTCTCGG ACCCCCTCCT CCTTCCCCAC 
CATGATGAAA CGGGTGGCCT GGTCCACTTA GAGGATCTCG AGAAGGATGC TCTGTTAGAG 
GAGGAGGAGT CTCAGCCAGT GGAGGTGCAC AAGCTGGTTC AGCATCCCCA GGAAGGTGCC 
AGGCTGTGTG AAAAGGACGT AAAGAGGAAA CTGGAGTTTG GGAACTCCAA ACCCCGCAGT 
GACTCCTTGC CTCAGGTGGA GGAGCTGGAG AAGGACGGTA GCCCAAGAAC GGGGAGGTGG 
AGGCGGGCCT CCACCCAGCT CGATAGAAGC TTGCTCGACC AGGAAAACCT AAATAACAAC 
AACAGCAAGA GGAGCTGTCC CGATGACTTG GAGCGCGATG CCATGTTTGG AATTCTCAGC 
AAAGTGAAGC CTCCCTACAC ATCCTGTGCC GACTGCATGT ACCCCACAGC TGGTGGGACT 
CCCGAGGCCT ACATGGAGCG ACACGAAGAC CCCAGCTCCT CTGCTATCTG CACCCAGCCA 
ACCTTCCTCC CCCATGTCAC GTCTTCCCCA ATGGCCCACG CGAGCAGCAG GTCCCGAGCT 
CCGGAGAGGC CGGCCTCGGG TCCAGCCAAC ACCTCTCCAT TCCTACTACC AGCAGGCTCC 
AGGAAGCCAG ATGTCAGTGG CTCTGGAGCC GGGGCTGCCC CGGAACCACC AGCAAGCCTT 
CTAGAGCCTT CCAGAGAGAC CTCAAAAGCC CTTCCAAAAT CTCTCCAGTT GAAGAATCCT 
CACTGTGACA AGAACGCTGC CAATATGGAG GTGTCGGCGA AGGAAGAACC GTCGCCTAAG 
AAAGATCCCA AGCCGGCTAA GGACCTGAGG CTTCTGTTCA GTAACGAGGC GGAGAAGCCC 
ACCACCAACA GCTACCTGAT GCAGCATCAG GAGTCCATCA TCCAACTGCA GAAGGCAGGC 
CTGGTCCGAA AGCACACCAA AGAGCTGGAG AGGTTGAAGA GCCTGCCTTC AGACTCACCA 
GCTGCCTGCA GGGACAGCGC CACCTGCAGG CTGGAGGCCA GCATCCCGGA GGAGGGTAGC 
CAGGAGCCCG CACACCCAGC CCTGTGCAGC CAAGCTGGGT CGGAAGAACA GCCTGTAGGG 
GGAACCTTGC AGAAGAGCCC CACGTCTACC CTCCCCCGTT TAGATCACAC CAGTAACTTC 
TCGAAGGACT TCCTGAAGAC CGTGTGTTAC ACCCCCACCT CCTCCTCCAT CAGCTCCAAC 
CTGACCCGGA GCTCCAGCAG CGACAGCATC CACAGCGTCC GAGGGAAGCC TGGGCTGGTG 
AAGCAGCGGG CGCAGGAGAT CGAGACGCGG CTGCGCCTGG CAGGCCTCAC CGTGTCGTCC 
CCGCTGAAAA GGTCCCATTC CCTTGCCAAG CTGGGAAGTC TCAACTTCTC CACGGAGGAC 
CTGTCCAGCG AGGCTGACAC ATCCACCATC GCTGACTCGC AGGACGCCAA GTGTGGTCTC 
TCCTCTTCCT TCTTGCCCGA ACCCCAGTCT GCGCCAAGGG ACCCCGCTGC AACCTCTAAA 
TCATCAGGGA AATCTGCCCC AGAACACTTG AAAAGCCCGT CGAGGGTAAA CAAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ssh1 ( XM_006530288.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530351.1 Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant X3, mRNA.

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CloneID	OMu35870
Clone ID Related Accession (Same CDS sequence)	XM_030254423.1 , XM_006530288.3 , XM_006530288.4
Accession Version	XM_006530288.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein phosphatase Slingshot homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530288.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAAG GAGCTGCCCT CTTCCTACAG CAGGGAAACA GCCCACAGGG CCAGCGGAGT 
CTTCAGCACC CTCACAAGCA TGCAGGTGAT CTGCCTCAGC ACCTGCAAGT GATGATCAAC 
CTCCTGCGTT GTGAAGACAG AATCAAGCTG GCCGTGCGCC TAGAGAGTGT CTGGACCGAC 
CGTGTCCGCT ACATGGTCGT GGTATACACC AGCGGGCGCC AGGACACCGA GGAGAATATT 
CTGCTGGGAG TTGACTTTTC CAGTAAGGAG AGCAAAAGCT GCACGATCGG AATGGTTCTT 
CGACTCTGGA GCGACACCAA GATTCACCTC GATGGGGACG GCGGGTTCAG TGTGAGCACA 
GCGGGCAGGA TGCACATATT CAAGCCAGTG TCTGTCCAGG CCATGTGGTC TGCCCTGCAA 
GTGCTTCACA AGGCCTGCGA AGTGGCCCGG AGGCATAACT ACTTCCCAGG AGGAGTGGCG 
CTCATCTGGG CCACCTACTA TGAGAGCTGC ATCAGCTCGG AGCAGAGCTG CATCAATGAG 
TGGAATGCCA TGCAGGACCT GGAGTCCACG CGGCCCGACT CCCCCGCGCT GTTTGTGGAC 
AAGCCAACCG AGGGCGAAAG AACTGAGCGC CTCATTAAAG CCAAACTCCG GAGCATCATG 
ATGAGCCAGG ACCTTGAAAA TGTGACTTCT AAGGAAATCC GAAATGAGCT GGAGAAGCAA 
ATGAACTGCA ACCTGAAGGA GTTCAAGGAA TTCATCGATA ACGAGATGCT GCTCATCCTG 
GGCCAGATGG ACAAGCCCTC CCTCATCTTT GACCATCTTT ATCTTGGCTC CGAGTGGAAT 
GCATCCAATC TGGAGGAGCT GCAGGGCTCA GGAGTTGACT ACATTTTAAA TGTCACTAGA 
GAAATAGACA ATTTTTTCCC CGGCTTGTTT GCGTACCATA ACATCCGCGT GTACGATGAG 
GAGACCACAG ACCTTCTTGC CCACTGGAAT GAAGCTTATC ATTTCATAAA CAAAGCGAAA 
AGGAATCATT CCAAGTGCCT GGTCCATTGC AAGATGGGCG TCAGCCGATC TGCGTCCACA 
GTCATTGCCT ACGCCATGAA GGAGTTCGGC TGGCCCCTGG AGAAAGCGTA TAACTACGTG 
AAGCAGAAAC GTAGTATCAC TCGGCCCAAC GCAGGCTTTA TGAGGCAGCT GTCTGAGTAC 
GAAGGCATCC TGGATGCAAG CAAACAGCGG CATAACAAAC TATGGCGCCA GCAGCCCACA 
GATGACACCA TCGCAGAGCC CAGTGAGTTC TTGCCAGAGA CCTTGGACGG AGCCCTGGAC 
GCTCAGCTGC CCTGTTTGGA TGATACCACC CACCCTGGGC TCCCGAGAAG TCTGGCCCCA 
GGAGGACCCG CTCTCCCCTG TTGTTTTCGA AGACTCTCGG ACCCCCTCCT CCTTCCCCAC 
CATGATGAAA CGGGTGGCCT GGTCCACTTA GAGGATCTCG AGAAGGATGC TCTGTTAGAG 
GAGGAGGAGT CTCAGCCAGT GGAGGTGCAC AAGCTGGTTC AGCATCCCCA GGAAGGTGCC 
AGGCTGTGTG AAAAGGACGT AAAGAGGAAA CTGGAGTTTG GGAACTCCAA ACCCCGCAGT 
GACTCCTTGC CTCAGGTGGA GGAGCTGGAG AAGGACGGTA GCCCAAGAAC GGGGAGGTGG 
AGGCGGGCCT CCACCCAGCT CGATAGAAGC TTGCTCGACC AGGAAAACCT AAATAACAAC 
AACAGCAAGA GGAGCTGTCC CGATGACTTG GAGCGCGATG CCATGTTTGG AATTCTCAGC 
AAAGTGAAGC CTCCCTACAC ATCCTGTGCC GACTGCATGT ACCCCACAGC TGGTGGGACT 
CCCGAGGCCT ACATGGAGCG ACACGAAGAC CCCAGCTCCT CTGCTATCTG CACCCAGCCA 
ACCTTCCTCC CCCATGTCAC GTCTTCCCCA ATGGCCCACG CGAGCAGCAG GTCCCGAGCT 
CCGGAGAGGC CGGCCTCGGG TCCAGCCAAC ACCTCTCCAT TCCTACTACC AGCAGGCTCC 
AGGAAGCCAG ATGTCAGTGG CTCTGGAGCC GGGGCTGCCC CGGAACCACC AGCAAGCCTT 
CTAGAGCCTT CCAGAGAGAC CTCAAAAGCC CTTCCAAAAT CTCTCCAGTT GAAGAATCCT 
CACTGTGACA AGAACGCTGC CAATATGGAG GTGTCGGCGA AGGAAGAACC GTCGCCTAAG 
AAAGATCCCA AGCCGGCTAA GGACCTGAGG CTTCTGTTCA GTAACGAGGC GGAGAAGCCC 
ACCACCAACA GCTACCTGAT GCAGCATCAG GAGTCCATCA TCCAACTGCA GAAGGCAGGC 
CTGGTCCGAA AGCACACCAA AGAGCTGGAG AGGTTGAAGA GCCTGCCTTC AGACTCACCA 
GCTGCCTGCA GGGACAGCGC CACCTGCAGG CTGGAGGCCA GCATCCCGGA GGAGGGTAGC 
CAGGAGCCCG CACACCCAGC CCTGTGCAGC CAAGCTGGGT CGGAAGAACA GCCTGTAGGG 
GGAACCTTGC AGAAGAGCCC CACGTCTACC CTCCCCCGTT TAGATCACAC CAGTAACTTC 
TCGAAGGACT TCCTGAAGAC CGTGTGTTAC ACCCCCACCT CCTCCTCCAT CAGCTCCAAC 
CTGACCCGGA GCTCCAGCAG CGACAGCATC CACAGCGTCC GAGGGAAGCC TGGGCTGGTG 
AAGCAGCGGG CGCAGGAGAT CGAGACGCGG CTGCGCCTGG CAGGCCTCAC CGTGTCGTCC 
CCGCTGAAAA GGTCCCATTC CCTTGCCAAG CTGGGAAGTC TCAACTTCTC CACGGAGGAC 
CTGTCCAGCG AGGCTGACAC ATCCACCATC GCTGACTCGC AGGACGCCAA GTGTGGTCTC 
TCCTCTTCCT TCTTGCCCGA ACCCCAGTCT GCGCCAAGGG ACCCCGCTGC AACCTCTAAA 
TCATCAGGGA AATCTGCCCC AGAACACTTG AAAAGCCCGT CGAGGGTAAA CAAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530351.1
CDS	438..3437
Translation	MVKGAALFLQQGNSPQGQRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESVWTDRVRYMVVVYTSGRQDTEENILLGVDFSSKESKSCTIGMVLRLWSDTKIHLDGDGGFSVSTAGRMHIFKPVSVQAMWSALQVLHKACEVARRHNYFPGGVALIWATYYESCISSEQSCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEIRNELEKQMNCNLKEFKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYILNVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAMKEFGWPLEKAYNYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQPTDDTIAEPSEFLPETLDGALDAQLPCLDDTTHPGLPRSLAPGGPALPCCFRRLSDPLLLPHHDETGGLVHLEDLEKDALLEEEESQPVEVHKLVQHPQEGARLCEKDVKRKLEFGNSKPRSDSLPQVEELEKDGSPRTGRWRRASTQLDRSLLDQENLNNNNSKRSCPDDLERDAMFGILSKVKPPYTSCADCMYPTAGGTPEAYMERHEDPSSSAICTQPTFLPHVTSSPMAHASSRSRAPERPASGPANTSPFLLPAGSRKPDVSGSGAGAAPEPPASLLEPSRETSKALPKSLQLKNPHCDKNAANMEVSAKEEPSPKKDPKPAKDLRLLFSNEAEKPTTNSYLMQHQESIIQLQKAGLVRKHTKELERLKSLPSDSPAACRDSATCRLEASIPEEGSQEPAHPALCSQAGSEEQPVGGTLQKSPTSTLPRLDHTSNFSKDFLKTVCYTPTSSSISSNLTRSSSSDSIHSVRGKPGLVKQRAQEIETRLRLAGLTVSSPLKRSHSLAKLGSLNFSTEDLSSEADTSTIADSQDAKCGLSSSFLPEPQSAPRDPAATSKSSGKSAPEHLKSPSRVNKS

Target ORF information:

RefSeq Version	XM_006530288.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530288.4

ORF Insert Sequence:

ATGGTGAAAG GAGCTGCCCT CTTCCTACAG CAGGGAAACA GCCCACAGGG CCAGCGGAGT 
CTTCAGCACC CTCACAAGCA TGCAGGTGAT CTGCCTCAGC ACCTGCAAGT GATGATCAAC 
CTCCTGCGTT GTGAAGACAG AATCAAGCTG GCCGTGCGCC TAGAGAGTGT CTGGACCGAC 
CGTGTCCGCT ACATGGTCGT GGTATACACC AGCGGGCGCC AGGACACCGA GGAGAATATT 
CTGCTGGGAG TTGACTTTTC CAGTAAGGAG AGCAAAAGCT GCACGATCGG AATGGTTCTT 
CGACTCTGGA GCGACACCAA GATTCACCTC GATGGGGACG GCGGGTTCAG TGTGAGCACA 
GCGGGCAGGA TGCACATATT CAAGCCAGTG TCTGTCCAGG CCATGTGGTC TGCCCTGCAA 
GTGCTTCACA AGGCCTGCGA AGTGGCCCGG AGGCATAACT ACTTCCCAGG AGGAGTGGCG 
CTCATCTGGG CCACCTACTA TGAGAGCTGC ATCAGCTCGG AGCAGAGCTG CATCAATGAG 
TGGAATGCCA TGCAGGACCT GGAGTCCACG CGGCCCGACT CCCCCGCGCT GTTTGTGGAC 
AAGCCAACCG AGGGCGAAAG AACTGAGCGC CTCATTAAAG CCAAACTCCG GAGCATCATG 
ATGAGCCAGG ACCTTGAAAA TGTGACTTCT AAGGAAATCC GAAATGAGCT GGAGAAGCAA 
ATGAACTGCA ACCTGAAGGA GTTCAAGGAA TTCATCGATA ACGAGATGCT GCTCATCCTG 
GGCCAGATGG ACAAGCCCTC CCTCATCTTT GACCATCTTT ATCTTGGCTC CGAGTGGAAT 
GCATCCAATC TGGAGGAGCT GCAGGGCTCA GGAGTTGACT ACATTTTAAA TGTCACTAGA 
GAAATAGACA ATTTTTTCCC CGGCTTGTTT GCGTACCATA ACATCCGCGT GTACGATGAG 
GAGACCACAG ACCTTCTTGC CCACTGGAAT GAAGCTTATC ATTTCATAAA CAAAGCGAAA 
AGGAATCATT CCAAGTGCCT GGTCCATTGC AAGATGGGCG TCAGCCGATC TGCGTCCACA 
GTCATTGCCT ACGCCATGAA GGAGTTCGGC TGGCCCCTGG AGAAAGCGTA TAACTACGTG 
AAGCAGAAAC GTAGTATCAC TCGGCCCAAC GCAGGCTTTA TGAGGCAGCT GTCTGAGTAC 
GAAGGCATCC TGGATGCAAG CAAACAGCGG CATAACAAAC TATGGCGCCA GCAGCCCACA 
GATGACACCA TCGCAGAGCC CAGTGAGTTC TTGCCAGAGA CCTTGGACGG AGCCCTGGAC 
GCTCAGCTGC CCTGTTTGGA TGATACCACC CACCCTGGGC TCCCGAGAAG TCTGGCCCCA 
GGAGGACCCG CTCTCCCCTG TTGTTTTCGA AGACTCTCGG ACCCCCTCCT CCTTCCCCAC 
CATGATGAAA CGGGTGGCCT GGTCCACTTA GAGGATCTCG AGAAGGATGC TCTGTTAGAG 
GAGGAGGAGT CTCAGCCAGT GGAGGTGCAC AAGCTGGTTC AGCATCCCCA GGAAGGTGCC 
AGGCTGTGTG AAAAGGACGT AAAGAGGAAA CTGGAGTTTG GGAACTCCAA ACCCCGCAGT 
GACTCCTTGC CTCAGGTGGA GGAGCTGGAG AAGGACGGTA GCCCAAGAAC GGGGAGGTGG 
AGGCGGGCCT CCACCCAGCT CGATAGAAGC TTGCTCGACC AGGAAAACCT AAATAACAAC 
AACAGCAAGA GGAGCTGTCC CGATGACTTG GAGCGCGATG CCATGTTTGG AATTCTCAGC 
AAAGTGAAGC CTCCCTACAC ATCCTGTGCC GACTGCATGT ACCCCACAGC TGGTGGGACT 
CCCGAGGCCT ACATGGAGCG ACACGAAGAC CCCAGCTCCT CTGCTATCTG CACCCAGCCA 
ACCTTCCTCC CCCATGTCAC GTCTTCCCCA ATGGCCCACG CGAGCAGCAG GTCCCGAGCT 
CCGGAGAGGC CGGCCTCGGG TCCAGCCAAC ACCTCTCCAT TCCTACTACC AGCAGGCTCC 
AGGAAGCCAG ATGTCAGTGG CTCTGGAGCC GGGGCTGCCC CGGAACCACC AGCAAGCCTT 
CTAGAGCCTT CCAGAGAGAC CTCAAAAGCC CTTCCAAAAT CTCTCCAGTT GAAGAATCCT 
CACTGTGACA AGAACGCTGC CAATATGGAG GTGTCGGCGA AGGAAGAACC GTCGCCTAAG 
AAAGATCCCA AGCCGGCTAA GGACCTGAGG CTTCTGTTCA GTAACGAGGC GGAGAAGCCC 
ACCACCAACA GCTACCTGAT GCAGCATCAG GAGTCCATCA TCCAACTGCA GAAGGCAGGC 
CTGGTCCGAA AGCACACCAA AGAGCTGGAG AGGTTGAAGA GCCTGCCTTC AGACTCACCA 
GCTGCCTGCA GGGACAGCGC CACCTGCAGG CTGGAGGCCA GCATCCCGGA GGAGGGTAGC 
CAGGAGCCCG CACACCCAGC CCTGTGCAGC CAAGCTGGGT CGGAAGAACA GCCTGTAGGG 
GGAACCTTGC AGAAGAGCCC CACGTCTACC CTCCCCCGTT TAGATCACAC CAGTAACTTC 
TCGAAGGACT TCCTGAAGAC CGTGTGTTAC ACCCCCACCT CCTCCTCCAT CAGCTCCAAC 
CTGACCCGGA GCTCCAGCAG CGACAGCATC CACAGCGTCC GAGGGAAGCC TGGGCTGGTG 
AAGCAGCGGG CGCAGGAGAT CGAGACGCGG CTGCGCCTGG CAGGCCTCAC CGTGTCGTCC 
CCGCTGAAAA GGTCCCATTC CCTTGCCAAG CTGGGAAGTC TCAACTTCTC CACGGAGGAC 
CTGTCCAGCG AGGCTGACAC ATCCACCATC GCTGACTCGC AGGACGCCAA GTGTGGTCTC 
TCCTCTTCCT TCTTGCCCGA ACCCCAGTCT GCGCCAAGGG ACCCCGCTGC AACCTCTAAA 
TCATCAGGGA AATCTGCCCC AGAACACTTG AAAAGCCCGT CGAGGGTAAA CAAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ssh1 ( NM_001363469.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350398.1 Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant 2, mRNA.

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CloneID	OMu84386
Clone ID Related Accession (Same CDS sequence)	NM_001363469.1
Accession Version	NM_001363469.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	protein phosphatase Slingshot homolog 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY282063.1, AK173144.1, AC145559.4, AK087447.1 and BB078364.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK173144.1, AK170787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGG TGACCCTTCA GCGCTCTCCC ACGCCCAGCG CCGCGTCTTC CTCCGCAAGC 
AACAGCGAGT TGGAGGCTGG CAGCGATGAA GAACGGAAAT TGAACCTCAG CTTGAGTGAG 
AGCTTTTTCA TGGTGAAAGG AGCTGCCCTC TTCCTACAGC AGGGAAACAG CCCACAGGGC 
CAGCGGAGTC TTCAGCACCC TCACAAGCAT GCAGGTGATC TGCCTCAGCA CCTGCAAGTG 
ATGATCAACC TCCTGCGTTG TGAAGACAGA ATCAAGCTGG CCGTGCGCCT AGAGAGTGTC 
TGGACCGACC GTGTCCGCTA CATGGTCGTG GTATACACCA GCGGGCGCCA GGACACCGAG 
GAGAATATTC TGCTGGGAGT TGACTTTTCC AGTAAGGAGA GCAAAAGCTG CACGATCGGA 
ATGGTTCTTC GACTCTGGAG CGACACCAAG ATTCACCTCG ATGGGGACGG GTCTGCCCTG 
CAAGTGCTTC ACAAGGCCTG CGAAGTGGCC CGGAGGCATA ACTACTTCCC AGGAGGAGTG 
GCGCTCATCT GGGCCACCTA CTATGAGAGC TGCATCAGCT CGGAGCAGAG CTGCATCAAT 
GAGTGGAATG CCATGCAGGA CCTGGAGTCC ACGCGGCCCG ACTCCCCCGC GCTGTTTGTG 
GACAAGCCAA CCGAGGGCGA AAGAACTGAG CGCCTCATTA AAGCCAAACT CCGGAGCATC 
ATGATGAGCC AGGACCTTGA AAATGTGACT TCTAAGGAAA TCCGAAATGA GCTGGAGAAG 
CAAATGAACT GCAACCTGAA GGAGTTCAAG GAATTCATCG ATAACGAGAT GCTGCTCATC 
CTGGGCCAGA TGGACAAGCC CTCCCTCATC TTTGACCATC TTTATCTTGG CTCCGAGTGG 
AATGCATCCA ATCTGGAGGA GCTGCAGGGC TCAGGAGTTG ACTACATTTT AAATGTCACT 
AGAGAAATAG ACAATTTTTT CCCCGGCTTG TTTGCGTACC ATAACATCCG CGTGTACGAT 
GAGGAGACCA CAGACCTTCT TGCCCACTGG AATGAAGCTT ATCATTTCAT AAACAAAGCG 
AAAAGGAATC ATTCCAAGTG CCTGGTCCAT TGCAAGATGG GCGTCAGCCG ATCTGCGTCC 
ACAGTCATTG CCTACGCCAT GAAGGAGTTC GGCTGGCCCC TGGAGAAAGC GTATAACTAC 
GTGAAGCAGA AACGTAGTAT CACTCGGCCC AACGCAGGCT TTATGAGGCA GCTGTCTGAG 
TACGAAGGCA TCCTGGATGC AAGCAAACAG CGGCATAACA AACTATGGCG CCAGCAGCCC 
ACAGATGACA CCATCGCAGA GCCCAGTGAG TTCTTGCCAG AGACCTTGGA CGGAGCCCTG 
GACGCTCAGC TGCCCTGTTT GGATGATACC ACCCACCCTG GGCTCCCGAG AAGTCTGGCC 
CCAGGAGGAC CCGCTCTCCC CTGTTGTTTT CGAAGACTCT CGGACCCCCT CCTCCTTCCC 
CACCATGATG AAACGGGTGG CCTGGTCCAC TTAGAGGATC TCGAGAAGGA TGCTCTGTTA 
GAGGAGGAGG AGTCTCAGCC AGTGGAGGTG CACAAGCTGG TTCAGCATCC CCAGGAAGGT 
GCCAGGCTGT GTGAAAAGGA CGTAAAGAGG AAACTGGAGT TTGGGAACTC CAAACCCCGC 
AGTGACTCCT TGCCTCAGGT GGAGGAGCTG GAGAAGGACG GTAGCCCAAG AACGGGGAGG 
TGGAGGCGGG CCTCCACCCA GCTCGATAGA AGCTTGCTCG ACCAGGAAAA CCTAAATAAC 
AACAACAGCA AGAGGAGCTG TCCCGATGAC TTGGAGCGCG ATGCCATGTT TGGAATTCTC 
AGCAAAGTGA AGCCTCCCTA CACATCCTGT GCCGACTGCA TGTACCCCAC AGCTGGTGGG 
ACTCCCGAGG CCTACATGGA GCGACACGAA GACCCCAGCT CCTCTGCTAT CTGCACCCAG 
CCAACCTTCC TCCCCCATGT CACGTCTTCC CCAATGGCCC ACGCGAGCAG CAGGTCCCGA 
GCTCCGGAGA GGCCGGCCTC GGGTCCAGCC AACACCTCTC CATTCCTACT ACCAGCAGGC 
TCCAGGAAGC CAGATGTCAG TGGCTCTGGA GCCGGGGCTG CCCCGGAACC ACCAGCAAGC 
CTTCTAGAGC CTTCCAGAGA GACCTCAAAA GCCCTTCCAA AATCTCTCCA GTTGAAGAAT 
CCTCACTGTG ACAAGAACGC TGCCAATATG GAGGTGTCGG CGAAGGAAGA ACCGTCGCCT 
AAGAAAGATC CCAAGCCGGC TAAGGACCTG AGGCTTCTGT TCAGTAACGA GGCGGAGAAG 
CCCACCACCA ACAGCTACCT GATGCAGCAT CAGGAGTCCA TCATCCAACT GCAGAAGGCA 
GGCCTGGTCC GAAAGCACAC CAAAGAGCTG GAGAGGTTGA AGAGCCTGCC TTCAGACTCA 
CCAGCTGCCT GCAGGGACAG CGCCACCTGC AGGCTGGAGG CCAGCATCCC GGAGGAGGGT 
AGCCAGGAGC CCGCACACCC AGCCCTGTGC AGCCAAGCTG GGTCGGAAGA ACAGCCTGTA 
GGGGGAACCT TGCAGAAGAG CCCCACGTCT ACCCTCCCCC GTTTAGATCA CACCAGTAAC 
TTCTCGAAGG ACTTCCTGAA GACCGTGTGT TACACCCCCA CCTCCTCCTC CATCAGCTCC 
AACCTGACCC GGAGCTCCAG CAGCGACAGC ATCCACAGCG TCCGAGGGAA GCCTGGGCTG 
GTGAAGCAGC GGGCGCAGGA GATCGAGACG CGGCTGCGCC TGGCAGGCCT CACCGTGTCG 
TCCCCGCTGA AAAGGTCCCA TTCCCTTGCC AAGCTGGGAA GTCTCAACTT CTCCACGGAG 
GACCTGTCCA GCGAGGCTGA CACATCCACC ATCGCTGACT CGCAGGACGC CAAGTGTGGT 
CTCTCCTCTT CCTTCTTGCC CGAACCCCAG TCTGCGCCAA GGGACCCCGC TGCAACCTCT 
AAATCATCAG GGAAATCTGC CCCAGAACAC TTGAAAAGCC CGTCGAGGGT AAACAAAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350398.1
CDS	59..3121
Translation	MALVTLQRSPTPSAASSSASNSELEAGSDEERKLNLSLSESFFMVKGAALFLQQGNSPQGQRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESVWTDRVRYMVVVYTSGRQDTEENILLGVDFSSKESKSCTIGMVLRLWSDTKIHLDGDGSALQVLHKACEVARRHNYFPGGVALIWATYYESCISSEQSCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEIRNELEKQMNCNLKEFKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYILNVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAMKEFGWPLEKAYNYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQPTDDTIAEPSEFLPETLDGALDAQLPCLDDTTHPGLPRSLAPGGPALPCCFRRLSDPLLLPHHDETGGLVHLEDLEKDALLEEEESQPVEVHKLVQHPQEGARLCEKDVKRKLEFGNSKPRSDSLPQVEELEKDGSPRTGRWRRASTQLDRSLLDQENLNNNNSKRSCPDDLERDAMFGILSKVKPPYTSCADCMYPTAGGTPEAYMERHEDPSSSAICTQPTFLPHVTSSPMAHASSRSRAPERPASGPANTSPFLLPAGSRKPDVSGSGAGAAPEPPASLLEPSRETSKALPKSLQLKNPHCDKNAANMEVSAKEEPSPKKDPKPAKDLRLLFSNEAEKPTTNSYLMQHQESIIQLQKAGLVRKHTKELERLKSLPSDSPAACRDSATCRLEASIPEEGSQEPAHPALCSQAGSEEQPVGGTLQKSPTSTLPRLDHTSNFSKDFLKTVCYTPTSSSISSNLTRSSSSDSIHSVRGKPGLVKQRAQEIETRLRLAGLTVSSPLKRSHSLAKLGSLNFSTEDLSSEADTSTIADSQDAKCGLSSSFLPEPQSAPRDPAATSKSSGKSAPEHLKSPSRVNKS

Target ORF information:

RefSeq Version	NM_001363469.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363469.1

ORF Insert Sequence:

ATGGCCTTGG TGACCCTTCA GCGCTCTCCC ACGCCCAGCG CCGCGTCTTC CTCCGCAAGC 
AACAGCGAGT TGGAGGCTGG CAGCGATGAA GAACGGAAAT TGAACCTCAG CTTGAGTGAG 
AGCTTTTTCA TGGTGAAAGG AGCTGCCCTC TTCCTACAGC AGGGAAACAG CCCACAGGGC 
CAGCGGAGTC TTCAGCACCC TCACAAGCAT GCAGGTGATC TGCCTCAGCA CCTGCAAGTG 
ATGATCAACC TCCTGCGTTG TGAAGACAGA ATCAAGCTGG CCGTGCGCCT AGAGAGTGTC 
TGGACCGACC GTGTCCGCTA CATGGTCGTG GTATACACCA GCGGGCGCCA GGACACCGAG 
GAGAATATTC TGCTGGGAGT TGACTTTTCC AGTAAGGAGA GCAAAAGCTG CACGATCGGA 
ATGGTTCTTC GACTCTGGAG CGACACCAAG ATTCACCTCG ATGGGGACGG GTCTGCCCTG 
CAAGTGCTTC ACAAGGCCTG CGAAGTGGCC CGGAGGCATA ACTACTTCCC AGGAGGAGTG 
GCGCTCATCT GGGCCACCTA CTATGAGAGC TGCATCAGCT CGGAGCAGAG CTGCATCAAT 
GAGTGGAATG CCATGCAGGA CCTGGAGTCC ACGCGGCCCG ACTCCCCCGC GCTGTTTGTG 
GACAAGCCAA CCGAGGGCGA AAGAACTGAG CGCCTCATTA AAGCCAAACT CCGGAGCATC 
ATGATGAGCC AGGACCTTGA AAATGTGACT TCTAAGGAAA TCCGAAATGA GCTGGAGAAG 
CAAATGAACT GCAACCTGAA GGAGTTCAAG GAATTCATCG ATAACGAGAT GCTGCTCATC 
CTGGGCCAGA TGGACAAGCC CTCCCTCATC TTTGACCATC TTTATCTTGG CTCCGAGTGG 
AATGCATCCA ATCTGGAGGA GCTGCAGGGC TCAGGAGTTG ACTACATTTT AAATGTCACT 
AGAGAAATAG ACAATTTTTT CCCCGGCTTG TTTGCGTACC ATAACATCCG CGTGTACGAT 
GAGGAGACCA CAGACCTTCT TGCCCACTGG AATGAAGCTT ATCATTTCAT AAACAAAGCG 
AAAAGGAATC ATTCCAAGTG CCTGGTCCAT TGCAAGATGG GCGTCAGCCG ATCTGCGTCC 
ACAGTCATTG CCTACGCCAT GAAGGAGTTC GGCTGGCCCC TGGAGAAAGC GTATAACTAC 
GTGAAGCAGA AACGTAGTAT CACTCGGCCC AACGCAGGCT TTATGAGGCA GCTGTCTGAG 
TACGAAGGCA TCCTGGATGC AAGCAAACAG CGGCATAACA AACTATGGCG CCAGCAGCCC 
ACAGATGACA CCATCGCAGA GCCCAGTGAG TTCTTGCCAG AGACCTTGGA CGGAGCCCTG 
GACGCTCAGC TGCCCTGTTT GGATGATACC ACCCACCCTG GGCTCCCGAG AAGTCTGGCC 
CCAGGAGGAC CCGCTCTCCC CTGTTGTTTT CGAAGACTCT CGGACCCCCT CCTCCTTCCC 
CACCATGATG AAACGGGTGG CCTGGTCCAC TTAGAGGATC TCGAGAAGGA TGCTCTGTTA 
GAGGAGGAGG AGTCTCAGCC AGTGGAGGTG CACAAGCTGG TTCAGCATCC CCAGGAAGGT 
GCCAGGCTGT GTGAAAAGGA CGTAAAGAGG AAACTGGAGT TTGGGAACTC CAAACCCCGC 
AGTGACTCCT TGCCTCAGGT GGAGGAGCTG GAGAAGGACG GTAGCCCAAG AACGGGGAGG 
TGGAGGCGGG CCTCCACCCA GCTCGATAGA AGCTTGCTCG ACCAGGAAAA CCTAAATAAC 
AACAACAGCA AGAGGAGCTG TCCCGATGAC TTGGAGCGCG ATGCCATGTT TGGAATTCTC 
AGCAAAGTGA AGCCTCCCTA CACATCCTGT GCCGACTGCA TGTACCCCAC AGCTGGTGGG 
ACTCCCGAGG CCTACATGGA GCGACACGAA GACCCCAGCT CCTCTGCTAT CTGCACCCAG 
CCAACCTTCC TCCCCCATGT CACGTCTTCC CCAATGGCCC ACGCGAGCAG CAGGTCCCGA 
GCTCCGGAGA GGCCGGCCTC GGGTCCAGCC AACACCTCTC CATTCCTACT ACCAGCAGGC 
TCCAGGAAGC CAGATGTCAG TGGCTCTGGA GCCGGGGCTG CCCCGGAACC ACCAGCAAGC 
CTTCTAGAGC CTTCCAGAGA GACCTCAAAA GCCCTTCCAA AATCTCTCCA GTTGAAGAAT 
CCTCACTGTG ACAAGAACGC TGCCAATATG GAGGTGTCGG CGAAGGAAGA ACCGTCGCCT 
AAGAAAGATC CCAAGCCGGC TAAGGACCTG AGGCTTCTGT TCAGTAACGA GGCGGAGAAG 
CCCACCACCA ACAGCTACCT GATGCAGCAT CAGGAGTCCA TCATCCAACT GCAGAAGGCA 
GGCCTGGTCC GAAAGCACAC CAAAGAGCTG GAGAGGTTGA AGAGCCTGCC TTCAGACTCA 
CCAGCTGCCT GCAGGGACAG CGCCACCTGC AGGCTGGAGG CCAGCATCCC GGAGGAGGGT 
AGCCAGGAGC CCGCACACCC AGCCCTGTGC AGCCAAGCTG GGTCGGAAGA ACAGCCTGTA 
GGGGGAACCT TGCAGAAGAG CCCCACGTCT ACCCTCCCCC GTTTAGATCA CACCAGTAAC 
TTCTCGAAGG ACTTCCTGAA GACCGTGTGT TACACCCCCA CCTCCTCCTC CATCAGCTCC 
AACCTGACCC GGAGCTCCAG CAGCGACAGC ATCCACAGCG TCCGAGGGAA GCCTGGGCTG 
GTGAAGCAGC GGGCGCAGGA GATCGAGACG CGGCTGCGCC TGGCAGGCCT CACCGTGTCG 
TCCCCGCTGA AAAGGTCCCA TTCCCTTGCC AAGCTGGGAA GTCTCAACTT CTCCACGGAG 
GACCTGTCCA GCGAGGCTGA CACATCCACC ATCGCTGACT CGCAGGACGC CAAGTGTGGT 
CTCTCCTCTT CCTTCTTGCC CGAACCCCAG TCTGCGCCAA GGGACCCCGC TGCAACCTCT 
AAATCATCAG GGAAATCTGC CCCAGAACAC TTGAAAAGCC CGTCGAGGGT AAACAAAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ssh1 ( XM_011248193.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246495.1 Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant X1, mRNA.

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CloneID	OMu73571
Clone ID Related Accession (Same CDS sequence)	XM_011248193.2 , XM_011248193.3
Accession Version	XM_011248193.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein phosphatase Slingshot homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248193.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATCC TTGTTTTGCT GGCTCCAGAC TTGCTGTGTA TTGAAGGGGT GACCTTGAAC 
TCTGATCACC TGGTTTCCCT CACCCGACTG AGTGCCAGGA TTACAGCTGT GGTGTGCATC 
ACCACACCTG GCTTGTGGCG GGCCAGAGAC CTTCATGATT GCTCAACAAG CATCCTACCA 
ACCGAGCTAC ATCCCGGCCT CTGCTTGAGT GAGAGCTTTT TCATGGTGAA AGGAGCTGCC 
CTCTTCCTAC AGCAGGGAAA CAGCCCACAG GGCCAGCGGA GTCTTCAGCA CCCTCACAAG 
CATGCAGGTG ATCTGCCTCA GCACCTGCAA GTGATGATCA ACCTCCTGCG TTGTGAAGAC 
AGAATCAAGC TGGCCGTGCG CCTAGAGAGT GTCTGGACCG ACCGTGTCCG CTACATGGTC 
GTGGTATACA CCAGCGGGCG CCAGGACACC GAGGAGAATA TTCTGCTGGG AGTTGACTTT 
TCCAGTAAGG AGAGCAAAAG CTGCACGATC GGAATGGTTC TTCGACTCTG GAGCGACACC 
AAGATTCACC TCGATGGGGA CGGCGGGTTC AGTGTGAGCA CAGCGGGCAG GATGCACATA 
TTCAAGCCAG TGTCTGTCCA GGCCATGTGG TCTGCCCTGC AAGTGCTTCA CAAGGCCTGC 
GAAGTGGCCC GGAGGCATAA CTACTTCCCA GGAGGAGTGG CGCTCATCTG GGCCACCTAC 
TATGAGAGCT GCATCAGCTC GGAGCAGAGC TGCATCAATG AGTGGAATGC CATGCAGGAC 
CTGGAGTCCA CGCGGCCCGA CTCCCCCGCG CTGTTTGTGG ACAAGCCAAC CGAGGGCGAA 
AGAACTGAGC GCCTCATTAA AGCCAAACTC CGGAGCATCA TGATGAGCCA GGACCTTGAA 
AATGTGACTT CTAAGGAAAT CCGAAATGAG CTGGAGAAGC AAATGAACTG CAACCTGAAG 
GAGTTCAAGG AATTCATCGA TAACGAGATG CTGCTCATCC TGGGCCAGAT GGACAAGCCC 
TCCCTCATCT TTGACCATCT TTATCTTGGC TCCGAGTGGA ATGCATCCAA TCTGGAGGAG 
CTGCAGGGCT CAGGAGTTGA CTACATTTTA AATGTCACTA GAGAAATAGA CAATTTTTTC 
CCCGGCTTGT TTGCGTACCA TAACATCCGC GTGTACGATG AGGAGACCAC AGACCTTCTT 
GCCCACTGGA ATGAAGCTTA TCATTTCATA AACAAAGCGA AAAGGAATCA TTCCAAGTGC 
CTGGTCCATT GCAAGATGGG CGTCAGCCGA TCTGCGTCCA CAGTCATTGC CTACGCCATG 
AAGGAGTTCG GCTGGCCCCT GGAGAAAGCG TATAACTACG TGAAGCAGAA ACGTAGTATC 
ACTCGGCCCA ACGCAGGCTT TATGAGGCAG CTGTCTGAGT ACGAAGGCAT CCTGGATGCA 
AGCAAACAGC GGCATAACAA ACTATGGCGC CAGCAGCCCA CAGATGACAC CATCGCAGAG 
CCCAGTGAGT TCTTGCCAGA GACCTTGGAC GGAGCCCTGG ACGCTCAGCT GCCCTGTTTG 
GATGATACCA CCCACCCTGG GCTCCCGAGA AGTCTGGCCC CAGGAGGACC CGCTCTCCCC 
TGTTGTTTTC GAAGACTCTC GGACCCCCTC CTCCTTCCCC ACCATGATGA AACGGGTGGC 
CTGGTCCACT TAGAGGATCT CGAGAAGGAT GCTCTGTTAG AGGAGGAGGA GTCTCAGCCA 
GTGGAGGTGC ACAAGCTGGT TCAGCATCCC CAGGAAGGTG CCAGGCTGTG TGAAAAGGAC 
GTAAAGAGGA AACTGGAGTT TGGGAACTCC AAACCCCGCA GTGACTCCTT GCCTCAGGTG 
GAGGAGCTGG AGAAGGACGG TAGCCCAAGA ACGGGGAGGT GGAGGCGGGC CTCCACCCAG 
CTCGATAGAA GCTTGCTCGA CCAGGAAAAC CTAAATAACA ACAACAGCAA GAGGAGCTGT 
CCCGATGACT TGGAGCGCGA TGCCATGTTT GGAATTCTCA GCAAAGTGAA GCCTCCCTAC 
ACATCCTGTG CCGACTGCAT GTACCCCACA GCTGGTGGGA CTCCCGAGGC CTACATGGAG 
CGACACGAAG ACCCCAGCTC CTCTGCTATC TGCACCCAGC CAACCTTCCT CCCCCATGTC 
ACGTCTTCCC CAATGGCCCA CGCGAGCAGC AGGTCCCGAG CTCCGGAGAG GCCGGCCTCG 
GGTCCAGCCA ACACCTCTCC ATTCCTACTA CCAGCAGGCT CCAGGAAGCC AGATGTCAGT 
GGCTCTGGAG CCGGGGCTGC CCCGGAACCA CCAGCAAGCC TTCTAGAGCC TTCCAGAGAG 
ACCTCAAAAG CCCTTCCAAA ATCTCTCCAG TTGAAGAATC CTCACTGTGA CAAGAACGCT 
GCCAATATGG AGGTGTCGGC GAAGGAAGAA CCGTCGCCTA AGAAAGATCC CAAGCCGGCT 
AAGGACCTGA GGCTTCTGTT CAGTAACGAG GCGGAGAAGC CCACCACCAA CAGCTACCTG 
ATGCAGCATC AGGAGTCCAT CATCCAACTG CAGAAGGCAG GCCTGGTCCG AAAGCACACC 
AAAGAGCTGG AGAGGTTGAA GAGCCTGCCT TCAGACTCAC CAGCTGCCTG CAGGGACAGC 
GCCACCTGCA GGCTGGAGGC CAGCATCCCG GAGGAGGGTA GCCAGGAGCC CGCACACCCA 
GCCCTGTGCA GCCAAGCTGG GTCGGAAGAA CAGCCTGTAG GGGGAACCTT GCAGAAGAGC 
CCCACGTCTA CCCTCCCCCG TTTAGATCAC ACCAGTAACT TCTCGAAGGA CTTCCTGAAG 
ACCGTGTGTT ACACCCCCAC CTCCTCCTCC ATCAGCTCCA ACCTGACCCG GAGCTCCAGC 
AGCGACAGCA TCCACAGCGT CCGAGGGAAG CCTGGGCTGG TGAAGCAGCG GGCGCAGGAG 
ATCGAGACGC GGCTGCGCCT GGCAGGCCTC ACCGTGTCGT CCCCGCTGAA AAGGTCCCAT 
TCCCTTGCCA AGCTGGGAAG TCTCAACTTC TCCACGGAGG ACCTGTCCAG CGAGGCTGAC 
ACATCCACCA TCGCTGACTC GCAGGACGCC AAGTGTGGTC TCTCCTCTTC CTTCTTGCCC 
GAACCCCAGT CTGCGCCAAG GGACCCCGCT GCAACCTCTA AATCATCAGG GAAATCTGCC 
CCAGAACACT TGAAAAGCCC GTCGAGGGTA AACAAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246495.1
CDS	100..3321
Translation	MMILVLLAPDLLCIEGVTLNSDHLVSLTRLSARITAVVCITTPGLWRARDLHDCSTSILPTELHPGLCLSESFFMVKGAALFLQQGNSPQGQRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESVWTDRVRYMVVVYTSGRQDTEENILLGVDFSSKESKSCTIGMVLRLWSDTKIHLDGDGGFSVSTAGRMHIFKPVSVQAMWSALQVLHKACEVARRHNYFPGGVALIWATYYESCISSEQSCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEIRNELEKQMNCNLKEFKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYILNVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAMKEFGWPLEKAYNYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQPTDDTIAEPSEFLPETLDGALDAQLPCLDDTTHPGLPRSLAPGGPALPCCFRRLSDPLLLPHHDETGGLVHLEDLEKDALLEEEESQPVEVHKLVQHPQEGARLCEKDVKRKLEFGNSKPRSDSLPQVEELEKDGSPRTGRWRRASTQLDRSLLDQENLNNNNSKRSCPDDLERDAMFGILSKVKPPYTSCADCMYPTAGGTPEAYMERHEDPSSSAICTQPTFLPHVTSSPMAHASSRSRAPERPASGPANTSPFLLPAGSRKPDVSGSGAGAAPEPPASLLEPSRETSKALPKSLQLKNPHCDKNAANMEVSAKEEPSPKKDPKPAKDLRLLFSNEAEKPTTNSYLMQHQESIIQLQKAGLVRKHTKELERLKSLPSDSPAACRDSATCRLEASIPEEGSQEPAHPALCSQAGSEEQPVGGTLQKSPTSTLPRLDHTSNFSKDFLKTVCYTPTSSSISSNLTRSSSSDSIHSVRGKPGLVKQRAQEIETRLRLAGLTVSSPLKRSHSLAKLGSLNFSTEDLSSEADTSTIADSQDAKCGLSSSFLPEPQSAPRDPAATSKSSGKSAPEHLKSPSRVNKS

Target ORF information:

RefSeq Version	XM_011248193.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248193.3

ORF Insert Sequence:

ATGATGATCC TTGTTTTGCT GGCTCCAGAC TTGCTGTGTA TTGAAGGGGT GACCTTGAAC 
TCTGATCACC TGGTTTCCCT CACCCGACTG AGTGCCAGGA TTACAGCTGT GGTGTGCATC 
ACCACACCTG GCTTGTGGCG GGCCAGAGAC CTTCATGATT GCTCAACAAG CATCCTACCA 
ACCGAGCTAC ATCCCGGCCT CTGCTTGAGT GAGAGCTTTT TCATGGTGAA AGGAGCTGCC 
CTCTTCCTAC AGCAGGGAAA CAGCCCACAG GGCCAGCGGA GTCTTCAGCA CCCTCACAAG 
CATGCAGGTG ATCTGCCTCA GCACCTGCAA GTGATGATCA ACCTCCTGCG TTGTGAAGAC 
AGAATCAAGC TGGCCGTGCG CCTAGAGAGT GTCTGGACCG ACCGTGTCCG CTACATGGTC 
GTGGTATACA CCAGCGGGCG CCAGGACACC GAGGAGAATA TTCTGCTGGG AGTTGACTTT 
TCCAGTAAGG AGAGCAAAAG CTGCACGATC GGAATGGTTC TTCGACTCTG GAGCGACACC 
AAGATTCACC TCGATGGGGA CGGCGGGTTC AGTGTGAGCA CAGCGGGCAG GATGCACATA 
TTCAAGCCAG TGTCTGTCCA GGCCATGTGG TCTGCCCTGC AAGTGCTTCA CAAGGCCTGC 
GAAGTGGCCC GGAGGCATAA CTACTTCCCA GGAGGAGTGG CGCTCATCTG GGCCACCTAC 
TATGAGAGCT GCATCAGCTC GGAGCAGAGC TGCATCAATG AGTGGAATGC CATGCAGGAC 
CTGGAGTCCA CGCGGCCCGA CTCCCCCGCG CTGTTTGTGG ACAAGCCAAC CGAGGGCGAA 
AGAACTGAGC GCCTCATTAA AGCCAAACTC CGGAGCATCA TGATGAGCCA GGACCTTGAA 
AATGTGACTT CTAAGGAAAT CCGAAATGAG CTGGAGAAGC AAATGAACTG CAACCTGAAG 
GAGTTCAAGG AATTCATCGA TAACGAGATG CTGCTCATCC TGGGCCAGAT GGACAAGCCC 
TCCCTCATCT TTGACCATCT TTATCTTGGC TCCGAGTGGA ATGCATCCAA TCTGGAGGAG 
CTGCAGGGCT CAGGAGTTGA CTACATTTTA AATGTCACTA GAGAAATAGA CAATTTTTTC 
CCCGGCTTGT TTGCGTACCA TAACATCCGC GTGTACGATG AGGAGACCAC AGACCTTCTT 
GCCCACTGGA ATGAAGCTTA TCATTTCATA AACAAAGCGA AAAGGAATCA TTCCAAGTGC 
CTGGTCCATT GCAAGATGGG CGTCAGCCGA TCTGCGTCCA CAGTCATTGC CTACGCCATG 
AAGGAGTTCG GCTGGCCCCT GGAGAAAGCG TATAACTACG TGAAGCAGAA ACGTAGTATC 
ACTCGGCCCA ACGCAGGCTT TATGAGGCAG CTGTCTGAGT ACGAAGGCAT CCTGGATGCA 
AGCAAACAGC GGCATAACAA ACTATGGCGC CAGCAGCCCA CAGATGACAC CATCGCAGAG 
CCCAGTGAGT TCTTGCCAGA GACCTTGGAC GGAGCCCTGG ACGCTCAGCT GCCCTGTTTG 
GATGATACCA CCCACCCTGG GCTCCCGAGA AGTCTGGCCC CAGGAGGACC CGCTCTCCCC 
TGTTGTTTTC GAAGACTCTC GGACCCCCTC CTCCTTCCCC ACCATGATGA AACGGGTGGC 
CTGGTCCACT TAGAGGATCT CGAGAAGGAT GCTCTGTTAG AGGAGGAGGA GTCTCAGCCA 
GTGGAGGTGC ACAAGCTGGT TCAGCATCCC CAGGAAGGTG CCAGGCTGTG TGAAAAGGAC 
GTAAAGAGGA AACTGGAGTT TGGGAACTCC AAACCCCGCA GTGACTCCTT GCCTCAGGTG 
GAGGAGCTGG AGAAGGACGG TAGCCCAAGA ACGGGGAGGT GGAGGCGGGC CTCCACCCAG 
CTCGATAGAA GCTTGCTCGA CCAGGAAAAC CTAAATAACA ACAACAGCAA GAGGAGCTGT 
CCCGATGACT TGGAGCGCGA TGCCATGTTT GGAATTCTCA GCAAAGTGAA GCCTCCCTAC 
ACATCCTGTG CCGACTGCAT GTACCCCACA GCTGGTGGGA CTCCCGAGGC CTACATGGAG 
CGACACGAAG ACCCCAGCTC CTCTGCTATC TGCACCCAGC CAACCTTCCT CCCCCATGTC 
ACGTCTTCCC CAATGGCCCA CGCGAGCAGC AGGTCCCGAG CTCCGGAGAG GCCGGCCTCG 
GGTCCAGCCA ACACCTCTCC ATTCCTACTA CCAGCAGGCT CCAGGAAGCC AGATGTCAGT 
GGCTCTGGAG CCGGGGCTGC CCCGGAACCA CCAGCAAGCC TTCTAGAGCC TTCCAGAGAG 
ACCTCAAAAG CCCTTCCAAA ATCTCTCCAG TTGAAGAATC CTCACTGTGA CAAGAACGCT 
GCCAATATGG AGGTGTCGGC GAAGGAAGAA CCGTCGCCTA AGAAAGATCC CAAGCCGGCT 
AAGGACCTGA GGCTTCTGTT CAGTAACGAG GCGGAGAAGC CCACCACCAA CAGCTACCTG 
ATGCAGCATC AGGAGTCCAT CATCCAACTG CAGAAGGCAG GCCTGGTCCG AAAGCACACC 
AAAGAGCTGG AGAGGTTGAA GAGCCTGCCT TCAGACTCAC CAGCTGCCTG CAGGGACAGC 
GCCACCTGCA GGCTGGAGGC CAGCATCCCG GAGGAGGGTA GCCAGGAGCC CGCACACCCA 
GCCCTGTGCA GCCAAGCTGG GTCGGAAGAA CAGCCTGTAG GGGGAACCTT GCAGAAGAGC 
CCCACGTCTA CCCTCCCCCG TTTAGATCAC ACCAGTAACT TCTCGAAGGA CTTCCTGAAG 
ACCGTGTGTT ACACCCCCAC CTCCTCCTCC ATCAGCTCCA ACCTGACCCG GAGCTCCAGC 
AGCGACAGCA TCCACAGCGT CCGAGGGAAG CCTGGGCTGG TGAAGCAGCG GGCGCAGGAG 
ATCGAGACGC GGCTGCGCCT GGCAGGCCTC ACCGTGTCGT CCCCGCTGAA AAGGTCCCAT 
TCCCTTGCCA AGCTGGGAAG TCTCAACTTC TCCACGGAGG ACCTGTCCAG CGAGGCTGAC 
ACATCCACCA TCGCTGACTC GCAGGACGCC AAGTGTGGTC TCTCCTCTTC CTTCTTGCCC 
GAACCCCAGT CTGCGCCAAG GGACCCCGCT GCAACCTCTA AATCATCAGG GAAATCTGCC 
CCAGAACACT TGAAAAGCCC GTCGAGGGTA AACAAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ssh1 ( NM_198109.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_932777.2 Mus musculus slingshot protein phosphatase 1 (Ssh1), mRNA.

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CloneID	OMu08870
Clone ID Related Accession (Same CDS sequence)	NM_198109.4 , NM_198109.5
Accession Version	NM_198109.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Mus musculus(house mouse)
Product	protein phosphatase Slingshot homolog 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC145559.4. On May 2, 2007 this sequence version replaced NM_198109.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB099287.1, AK089308.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTTGG TGACCCTTCA GCGCTCTCCC ACGCCCAGCG CCGCGTCTTC CTCCGCAAGC 
AACAGCGAGT TGGAGGCTGG CAGCGATGAA GAACGGAAAT TGAACCTCAG CTTGAGTGAG 
AGCTTTTTCA TGGTGAAAGG AGCTGCCCTC TTCCTACAGC AGGGAAACAG CCCACAGGGC 
CAGCGGAGTC TTCAGCACCC TCACAAGCAT GCAGGTGATC TGCCTCAGCA CCTGCAAGTG 
ATGATCAACC TCCTGCGTTG TGAAGACAGA ATCAAGCTGG CCGTGCGCCT AGAGAGTGTC 
TGGACCGACC GTGTCCGCTA CATGGTCGTG GTATACACCA GCGGGCGCCA GGACACCGAG 
GAGAATATTC TGCTGGGAGT TGACTTTTCC AGTAAGGAGA GCAAAAGCTG CACGATCGGA 
ATGGTTCTTC GACTCTGGAG CGACACCAAG ATTCACCTCG ATGGGGACGG CGGGTTCAGT 
GTGAGCACAG CGGGCAGGAT GCACATATTC AAGCCAGTGT CTGTCCAGGC CATGTGGTCT 
GCCCTGCAAG TGCTTCACAA GGCCTGCGAA GTGGCCCGGA GGCATAACTA CTTCCCAGGA 
GGAGTGGCGC TCATCTGGGC CACCTACTAT GAGAGCTGCA TCAGCTCGGA GCAGAGCTGC 
ATCAATGAGT GGAATGCCAT GCAGGACCTG GAGTCCACGC GGCCCGACTC CCCCGCGCTG 
TTTGTGGACA AGCCAACCGA GGGCGAAAGA ACTGAGCGCC TCATTAAAGC CAAACTCCGG 
AGCATCATGA TGAGCCAGGA CCTTGAAAAT GTGACTTCTA AGGAAATCCG AAATGAGCTG 
GAGAAGCAAA TGAACTGCAA CCTGAAGGAG TTCAAGGAAT TCATCGATAA CGAGATGCTG 
CTCATCCTGG GCCAGATGGA CAAGCCCTCC CTCATCTTTG ACCATCTTTA TCTTGGCTCC 
GAGTGGAATG CATCCAATCT GGAGGAGCTG CAGGGCTCAG GAGTTGACTA CATTTTAAAT 
GTCACTAGAG AAATAGACAA TTTTTTCCCC GGCTTGTTTG CGTACCATAA CATCCGCGTG 
TACGATGAGG AGACCACAGA CCTTCTTGCC CACTGGAATG AAGCTTATCA TTTCATAAAC 
AAAGCGAAAA GGAATCATTC CAAGTGCCTG GTCCATTGCA AGATGGGCGT CAGCCGATCT 
GCGTCCACAG TCATTGCCTA CGCCATGAAG GAGTTCGGCT GGCCCCTGGA GAAAGCGTAT 
AACTACGTGA AGCAGAAACG TAGTATCACT CGGCCCAACG CAGGCTTTAT GAGGCAGCTG 
TCTGAGTACG AAGGCATCCT GGATGCAAGC AAACAGCGGC ATAACAAACT ATGGCGCCAG 
CAGCCCACAG ATGACACCAT CGCAGAGCCC AGTGAGTTCT TGCCAGAGAC CTTGGACGGA 
GCCCTGGACG CTCAGCTGCC CTGTTTGGAT GATACCACCC ACCCTGGGCT CCCGAGAAGT 
CTGGCCCCAG GAGGACCCGC TCTCCCCTGT TGTTTTCGAA GACTCTCGGA CCCCCTCCTC 
CTTCCCCACC ATGATGAAAC GGGTGGCCTG GTCCACTTAG AGGATCTCGA GAAGGATGCT 
CTGTTAGAGG AGGAGGAGTC TCAGCCAGTG GAGGTGCACA AGCTGGTTCA GCATCCCCAG 
GAAGGTGCCA GGCTGTGTGA AAAGGACGTA AAGAGGAAAC TGGAGTTTGG GAACTCCAAA 
CCCCGCAGTG ACTCCTTGCC TCAGGTGGAG GAGCTGGAGA AGGACGGTAG CCCAAGAACG 
GGGAGGTGGA GGCGGGCCTC CACCCAGCTC GATAGAAGCT TGCTCGACCA GGAAAACCTA 
AATAACAACA ACAGCAAGAG GAGCTGTCCC GATGACTTGG AGCGCGATGC CATGTTTGGA 
ATTCTCAGCA AAGTGAAGCC TCCCTACACA TCCTGTGCCG ACTGCATGTA CCCCACAGCT 
GGTGGGACTC CCGAGGCCTA CATGGAGCGA CACGAAGACC CCAGCTCCTC TGCTATCTGC 
ACCCAGCCAA CCTTCCTCCC CCATGTCACG TCTTCCCCAA TGGCCCACGC GAGCAGCAGG 
TCCCGAGCTC CGGAGAGGCC GGCCTCGGGT CCAGCCAACA CCTCTCCATT CCTACTACCA 
GCAGGCTCCA GGAAGCCAGA TGTCAGTGGC TCTGGAGCCG GGGCTGCCCC GGAACCACCA 
GCAAGCCTTC TAGAGCCTTC CAGAGAGACC TCAAAAGCCC TTCCAAAATC TCTCCAGTTG 
AAGAATCCTC ACTGTGACAA GAACGCTGCC AATATGGAGG TGTCGGCGAA GGAAGAACCG 
TCGCCTAAGA AAGATCCCAA GCCGGCTAAG GACCTGAGGC TTCTGTTCAG TAACGAGGCG 
GAGAAGCCCA CCACCAACAG CTACCTGATG CAGCATCAGG AGTCCATCAT CCAACTGCAG 
AAGGCAGGCC TGGTCCGAAA GCACACCAAA GAGCTGGAGA GGTTGAAGAG CCTGCCTTCA 
GACTCACCAG CTGCCTGCAG GGACAGCGCC ACCTGCAGGC TGGAGGCCAG CATCCCGGAG 
GAGGGTAGCC AGGAGCCCGC ACACCCAGCC CTGTGCAGCC AAGCTGGGTC GGAAGAACAG 
CCTGTAGGGG GAACCTTGCA GAAGAGCCCC ACGTCTACCC TCCCCCGTTT AGATCACACC 
AGTAACTTCT CGAAGGACTT CCTGAAGACC GTGTGTTACA CCCCCACCTC CTCCTCCATC 
AGCTCCAACC TGACCCGGAG CTCCAGCAGC GACAGCATCC ACAGCGTCCG AGGGAAGCCT 
GGGCTGGTGA AGCAGCGGGC GCAGGAGATC GAGACGCGGC TGCGCCTGGC AGGCCTCACC 
GTGTCGTCCC CGCTGAAAAG GTCCCATTCC CTTGCCAAGC TGGGAAGTCT CAACTTCTCC 
ACGGAGGACC TGTCCAGCGA GGCTGACACA TCCACCATCG CTGACTCGCA GGACGCCAAG 
TGTGGTCTCT CCTCTTCCTT CTTGCCCGAA CCCCAGTCTG CGCCAAGGGA CCCCGCTGCA 
ACCTCTAAAT CATCAGGGAA ATCTGCCCCA GAACACTTGA AAAGCCCGTC GAGGGTAAAC 
AAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_932777.2
CDS	26..3154
Translation	MALVTLQRSPTPSAASSSASNSELEAGSDEERKLNLSLSESFFMVKGAALFLQQGNSPQGQRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESVWTDRVRYMVVVYTSGRQDTEENILLGVDFSSKESKSCTIGMVLRLWSDTKIHLDGDGGFSVSTAGRMHIFKPVSVQAMWSALQVLHKACEVARRHNYFPGGVALIWATYYESCISSEQSCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEIRNELEKQMNCNLKEFKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYILNVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAMKEFGWPLEKAYNYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQPTDDTIAEPSEFLPETLDGALDAQLPCLDDTTHPGLPRSLAPGGPALPCCFRRLSDPLLLPHHDETGGLVHLEDLEKDALLEEEESQPVEVHKLVQHPQEGARLCEKDVKRKLEFGNSKPRSDSLPQVEELEKDGSPRTGRWRRASTQLDRSLLDQENLNNNNSKRSCPDDLERDAMFGILSKVKPPYTSCADCMYPTAGGTPEAYMERHEDPSSSAICTQPTFLPHVTSSPMAHASSRSRAPERPASGPANTSPFLLPAGSRKPDVSGSGAGAAPEPPASLLEPSRETSKALPKSLQLKNPHCDKNAANMEVSAKEEPSPKKDPKPAKDLRLLFSNEAEKPTTNSYLMQHQESIIQLQKAGLVRKHTKELERLKSLPSDSPAACRDSATCRLEASIPEEGSQEPAHPALCSQAGSEEQPVGGTLQKSPTSTLPRLDHTSNFSKDFLKTVCYTPTSSSISSNLTRSSSSDSIHSVRGKPGLVKQRAQEIETRLRLAGLTVSSPLKRSHSLAKLGSLNFSTEDLSSEADTSTIADSQDAKCGLSSSFLPEPQSAPRDPAATSKSSGKSAPEHLKSPSRVNKS

Target ORF information:

RefSeq Version	NM_198109.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus slingshot protein phosphatase 1 (Ssh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198109.4

ORF Insert Sequence:

ATGGCCTTGG TGACCCTTCA GCGCTCTCCC ACGCCCAGCG CCGCGTCTTC CTCCGCAAGC 
AACAGCGAGT TGGAGGCTGG CAGCGATGAA GAACGGAAAT TGAACCTCAG CTTGAGTGAG 
AGCTTTTTCA TGGTGAAAGG AGCTGCCCTC TTCCTACAGC AGGGAAACAG CCCACAGGGC 
CAGCGGAGTC TTCAGCACCC TCACAAGCAT GCAGGTGATC TGCCTCAGCA CCTGCAAGTG 
ATGATCAACC TCCTGCGTTG TGAAGACAGA ATCAAGCTGG CCGTGCGCCT AGAGAGTGTC 
TGGACCGACC GTGTCCGCTA CATGGTCGTG GTATACACCA GCGGGCGCCA GGACACCGAG 
GAGAATATTC TGCTGGGAGT TGACTTTTCC AGTAAGGAGA GCAAAAGCTG CACGATCGGA 
ATGGTTCTTC GACTCTGGAG CGACACCAAG ATTCACCTCG ATGGGGACGG CGGGTTCAGT 
GTGAGCACAG CGGGCAGGAT GCACATATTC AAGCCAGTGT CTGTCCAGGC CATGTGGTCT 
GCCCTGCAAG TGCTTCACAA GGCCTGCGAA GTGGCCCGGA GGCATAACTA CTTCCCAGGA 
GGAGTGGCGC TCATCTGGGC CACCTACTAT GAGAGCTGCA TCAGCTCGGA GCAGAGCTGC 
ATCAATGAGT GGAATGCCAT GCAGGACCTG GAGTCCACGC GGCCCGACTC CCCCGCGCTG 
TTTGTGGACA AGCCAACCGA GGGCGAAAGA ACTGAGCGCC TCATTAAAGC CAAACTCCGG 
AGCATCATGA TGAGCCAGGA CCTTGAAAAT GTGACTTCTA AGGAAATCCG AAATGAGCTG 
GAGAAGCAAA TGAACTGCAA CCTGAAGGAG TTCAAGGAAT TCATCGATAA CGAGATGCTG 
CTCATCCTGG GCCAGATGGA CAAGCCCTCC CTCATCTTTG ACCATCTTTA TCTTGGCTCC 
GAGTGGAATG CATCCAATCT GGAGGAGCTG CAGGGCTCAG GAGTTGACTA CATTTTAAAT 
GTCACTAGAG AAATAGACAA TTTTTTCCCC GGCTTGTTTG CGTACCATAA CATCCGCGTG 
TACGATGAGG AGACCACAGA CCTTCTTGCC CACTGGAATG AAGCTTATCA TTTCATAAAC 
AAAGCGAAAA GGAATCATTC CAAGTGCCTG GTCCATTGCA AGATGGGCGT CAGCCGATCT 
GCGTCCACAG TCATTGCCTA CGCCATGAAG GAGTTCGGCT GGCCCCTGGA GAAAGCGTAT 
AACTACGTGA AGCAGAAACG TAGTATCACT CGGCCCAACG CAGGCTTTAT GAGGCAGCTG 
TCTGAGTACG AAGGCATCCT GGATGCAAGC AAACAGCGGC ATAACAAACT ATGGCGCCAG 
CAGCCCACAG ATGACACCAT CGCAGAGCCC AGTGAGTTCT TGCCAGAGAC CTTGGACGGA 
GCCCTGGACG CTCAGCTGCC CTGTTTGGAT GATACCACCC ACCCTGGGCT CCCGAGAAGT 
CTGGCCCCAG GAGGACCCGC TCTCCCCTGT TGTTTTCGAA GACTCTCGGA CCCCCTCCTC 
CTTCCCCACC ATGATGAAAC GGGTGGCCTG GTCCACTTAG AGGATCTCGA GAAGGATGCT 
CTGTTAGAGG AGGAGGAGTC TCAGCCAGTG GAGGTGCACA AGCTGGTTCA GCATCCCCAG 
GAAGGTGCCA GGCTGTGTGA AAAGGACGTA AAGAGGAAAC TGGAGTTTGG GAACTCCAAA 
CCCCGCAGTG ACTCCTTGCC TCAGGTGGAG GAGCTGGAGA AGGACGGTAG CCCAAGAACG 
GGGAGGTGGA GGCGGGCCTC CACCCAGCTC GATAGAAGCT TGCTCGACCA GGAAAACCTA 
AATAACAACA ACAGCAAGAG GAGCTGTCCC GATGACTTGG AGCGCGATGC CATGTTTGGA 
ATTCTCAGCA AAGTGAAGCC TCCCTACACA TCCTGTGCCG ACTGCATGTA CCCCACAGCT 
GGTGGGACTC CCGAGGCCTA CATGGAGCGA CACGAAGACC CCAGCTCCTC TGCTATCTGC 
ACCCAGCCAA CCTTCCTCCC CCATGTCACG TCTTCCCCAA TGGCCCACGC GAGCAGCAGG 
TCCCGAGCTC CGGAGAGGCC GGCCTCGGGT CCAGCCAACA CCTCTCCATT CCTACTACCA 
GCAGGCTCCA GGAAGCCAGA TGTCAGTGGC TCTGGAGCCG GGGCTGCCCC GGAACCACCA 
GCAAGCCTTC TAGAGCCTTC CAGAGAGACC TCAAAAGCCC TTCCAAAATC TCTCCAGTTG 
AAGAATCCTC ACTGTGACAA GAACGCTGCC AATATGGAGG TGTCGGCGAA GGAAGAACCG 
TCGCCTAAGA AAGATCCCAA GCCGGCTAAG GACCTGAGGC TTCTGTTCAG TAACGAGGCG 
GAGAAGCCCA CCACCAACAG CTACCTGATG CAGCATCAGG AGTCCATCAT CCAACTGCAG 
AAGGCAGGCC TGGTCCGAAA GCACACCAAA GAGCTGGAGA GGTTGAAGAG CCTGCCTTCA 
GACTCACCAG CTGCCTGCAG GGACAGCGCC ACCTGCAGGC TGGAGGCCAG CATCCCGGAG 
GAGGGTAGCC AGGAGCCCGC ACACCCAGCC CTGTGCAGCC AAGCTGGGTC GGAAGAACAG 
CCTGTAGGGG GAACCTTGCA GAAGAGCCCC ACGTCTACCC TCCCCCGTTT AGATCACACC 
AGTAACTTCT CGAAGGACTT CCTGAAGACC GTGTGTTACA CCCCCACCTC CTCCTCCATC 
AGCTCCAACC TGACCCGGAG CTCCAGCAGC GACAGCATCC ACAGCGTCCG AGGGAAGCCT 
GGGCTGGTGA AGCAGCGGGC GCAGGAGATC GAGACGCGGC TGCGCCTGGC AGGCCTCACC 
GTGTCGTCCC CGCTGAAAAG GTCCCATTCC CTTGCCAAGC TGGGAAGTCT CAACTTCTCC 
ACGGAGGACC TGTCCAGCGA GGCTGACACA TCCACCATCG CTGACTCGCA GGACGCCAAG 
TGTGGTCTCT CCTCTTCCTT CTTGCCCGAA CCCCAGTCTG CGCCAAGGGA CCCCGCTGCA 
ACCTCTAAAT CATCAGGGAA ATCTGCCCCA GAACACTTGA AAAGCCCGTC GAGGGTAAAC 
AAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ssh1 ( NM_198109.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_932777.2 Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant 1, mRNA.

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CloneID	OMu08870
Clone ID Related Accession (Same CDS sequence)	NM_198109.4 , NM_198109.5
Accession Version	NM_198109.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	protein phosphatase Slingshot homolog 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC145559.4. On May 19, 2018 this sequence version replaced NM_198109.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK171210.1, AK089308.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGG TGACCCTTCA GCGCTCTCCC ACGCCCAGCG CCGCGTCTTC CTCCGCAAGC 
AACAGCGAGT TGGAGGCTGG CAGCGATGAA GAACGGAAAT TGAACCTCAG CTTGAGTGAG 
AGCTTTTTCA TGGTGAAAGG AGCTGCCCTC TTCCTACAGC AGGGAAACAG CCCACAGGGC 
CAGCGGAGTC TTCAGCACCC TCACAAGCAT GCAGGTGATC TGCCTCAGCA CCTGCAAGTG 
ATGATCAACC TCCTGCGTTG TGAAGACAGA ATCAAGCTGG CCGTGCGCCT AGAGAGTGTC 
TGGACCGACC GTGTCCGCTA CATGGTCGTG GTATACACCA GCGGGCGCCA GGACACCGAG 
GAGAATATTC TGCTGGGAGT TGACTTTTCC AGTAAGGAGA GCAAAAGCTG CACGATCGGA 
ATGGTTCTTC GACTCTGGAG CGACACCAAG ATTCACCTCG ATGGGGACGG CGGGTTCAGT 
GTGAGCACAG CGGGCAGGAT GCACATATTC AAGCCAGTGT CTGTCCAGGC CATGTGGTCT 
GCCCTGCAAG TGCTTCACAA GGCCTGCGAA GTGGCCCGGA GGCATAACTA CTTCCCAGGA 
GGAGTGGCGC TCATCTGGGC CACCTACTAT GAGAGCTGCA TCAGCTCGGA GCAGAGCTGC 
ATCAATGAGT GGAATGCCAT GCAGGACCTG GAGTCCACGC GGCCCGACTC CCCCGCGCTG 
TTTGTGGACA AGCCAACCGA GGGCGAAAGA ACTGAGCGCC TCATTAAAGC CAAACTCCGG 
AGCATCATGA TGAGCCAGGA CCTTGAAAAT GTGACTTCTA AGGAAATCCG AAATGAGCTG 
GAGAAGCAAA TGAACTGCAA CCTGAAGGAG TTCAAGGAAT TCATCGATAA CGAGATGCTG 
CTCATCCTGG GCCAGATGGA CAAGCCCTCC CTCATCTTTG ACCATCTTTA TCTTGGCTCC 
GAGTGGAATG CATCCAATCT GGAGGAGCTG CAGGGCTCAG GAGTTGACTA CATTTTAAAT 
GTCACTAGAG AAATAGACAA TTTTTTCCCC GGCTTGTTTG CGTACCATAA CATCCGCGTG 
TACGATGAGG AGACCACAGA CCTTCTTGCC CACTGGAATG AAGCTTATCA TTTCATAAAC 
AAAGCGAAAA GGAATCATTC CAAGTGCCTG GTCCATTGCA AGATGGGCGT CAGCCGATCT 
GCGTCCACAG TCATTGCCTA CGCCATGAAG GAGTTCGGCT GGCCCCTGGA GAAAGCGTAT 
AACTACGTGA AGCAGAAACG TAGTATCACT CGGCCCAACG CAGGCTTTAT GAGGCAGCTG 
TCTGAGTACG AAGGCATCCT GGATGCAAGC AAACAGCGGC ATAACAAACT ATGGCGCCAG 
CAGCCCACAG ATGACACCAT CGCAGAGCCC AGTGAGTTCT TGCCAGAGAC CTTGGACGGA 
GCCCTGGACG CTCAGCTGCC CTGTTTGGAT GATACCACCC ACCCTGGGCT CCCGAGAAGT 
CTGGCCCCAG GAGGACCCGC TCTCCCCTGT TGTTTTCGAA GACTCTCGGA CCCCCTCCTC 
CTTCCCCACC ATGATGAAAC GGGTGGCCTG GTCCACTTAG AGGATCTCGA GAAGGATGCT 
CTGTTAGAGG AGGAGGAGTC TCAGCCAGTG GAGGTGCACA AGCTGGTTCA GCATCCCCAG 
GAAGGTGCCA GGCTGTGTGA AAAGGACGTA AAGAGGAAAC TGGAGTTTGG GAACTCCAAA 
CCCCGCAGTG ACTCCTTGCC TCAGGTGGAG GAGCTGGAGA AGGACGGTAG CCCAAGAACG 
GGGAGGTGGA GGCGGGCCTC CACCCAGCTC GATAGAAGCT TGCTCGACCA GGAAAACCTA 
AATAACAACA ACAGCAAGAG GAGCTGTCCC GATGACTTGG AGCGCGATGC CATGTTTGGA 
ATTCTCAGCA AAGTGAAGCC TCCCTACACA TCCTGTGCCG ACTGCATGTA CCCCACAGCT 
GGTGGGACTC CCGAGGCCTA CATGGAGCGA CACGAAGACC CCAGCTCCTC TGCTATCTGC 
ACCCAGCCAA CCTTCCTCCC CCATGTCACG TCTTCCCCAA TGGCCCACGC GAGCAGCAGG 
TCCCGAGCTC CGGAGAGGCC GGCCTCGGGT CCAGCCAACA CCTCTCCATT CCTACTACCA 
GCAGGCTCCA GGAAGCCAGA TGTCAGTGGC TCTGGAGCCG GGGCTGCCCC GGAACCACCA 
GCAAGCCTTC TAGAGCCTTC CAGAGAGACC TCAAAAGCCC TTCCAAAATC TCTCCAGTTG 
AAGAATCCTC ACTGTGACAA GAACGCTGCC AATATGGAGG TGTCGGCGAA GGAAGAACCG 
TCGCCTAAGA AAGATCCCAA GCCGGCTAAG GACCTGAGGC TTCTGTTCAG TAACGAGGCG 
GAGAAGCCCA CCACCAACAG CTACCTGATG CAGCATCAGG AGTCCATCAT CCAACTGCAG 
AAGGCAGGCC TGGTCCGAAA GCACACCAAA GAGCTGGAGA GGTTGAAGAG CCTGCCTTCA 
GACTCACCAG CTGCCTGCAG GGACAGCGCC ACCTGCAGGC TGGAGGCCAG CATCCCGGAG 
GAGGGTAGCC AGGAGCCCGC ACACCCAGCC CTGTGCAGCC AAGCTGGGTC GGAAGAACAG 
CCTGTAGGGG GAACCTTGCA GAAGAGCCCC ACGTCTACCC TCCCCCGTTT AGATCACACC 
AGTAACTTCT CGAAGGACTT CCTGAAGACC GTGTGTTACA CCCCCACCTC CTCCTCCATC 
AGCTCCAACC TGACCCGGAG CTCCAGCAGC GACAGCATCC ACAGCGTCCG AGGGAAGCCT 
GGGCTGGTGA AGCAGCGGGC GCAGGAGATC GAGACGCGGC TGCGCCTGGC AGGCCTCACC 
GTGTCGTCCC CGCTGAAAAG GTCCCATTCC CTTGCCAAGC TGGGAAGTCT CAACTTCTCC 
ACGGAGGACC TGTCCAGCGA GGCTGACACA TCCACCATCG CTGACTCGCA GGACGCCAAG 
TGTGGTCTCT CCTCTTCCTT CTTGCCCGAA CCCCAGTCTG CGCCAAGGGA CCCCGCTGCA 
ACCTCTAAAT CATCAGGGAA ATCTGCCCCA GAACACTTGA AAAGCCCGTC GAGGGTAAAC 
AAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_932777.2
CDS	59..3187
Translation	MALVTLQRSPTPSAASSSASNSELEAGSDEERKLNLSLSESFFMVKGAALFLQQGNSPQGQRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESVWTDRVRYMVVVYTSGRQDTEENILLGVDFSSKESKSCTIGMVLRLWSDTKIHLDGDGGFSVSTAGRMHIFKPVSVQAMWSALQVLHKACEVARRHNYFPGGVALIWATYYESCISSEQSCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEIRNELEKQMNCNLKEFKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYILNVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAMKEFGWPLEKAYNYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQPTDDTIAEPSEFLPETLDGALDAQLPCLDDTTHPGLPRSLAPGGPALPCCFRRLSDPLLLPHHDETGGLVHLEDLEKDALLEEEESQPVEVHKLVQHPQEGARLCEKDVKRKLEFGNSKPRSDSLPQVEELEKDGSPRTGRWRRASTQLDRSLLDQENLNNNNSKRSCPDDLERDAMFGILSKVKPPYTSCADCMYPTAGGTPEAYMERHEDPSSSAICTQPTFLPHVTSSPMAHASSRSRAPERPASGPANTSPFLLPAGSRKPDVSGSGAGAAPEPPASLLEPSRETSKALPKSLQLKNPHCDKNAANMEVSAKEEPSPKKDPKPAKDLRLLFSNEAEKPTTNSYLMQHQESIIQLQKAGLVRKHTKELERLKSLPSDSPAACRDSATCRLEASIPEEGSQEPAHPALCSQAGSEEQPVGGTLQKSPTSTLPRLDHTSNFSKDFLKTVCYTPTSSSISSNLTRSSSSDSIHSVRGKPGLVKQRAQEIETRLRLAGLTVSSPLKRSHSLAKLGSLNFSTEDLSSEADTSTIADSQDAKCGLSSSFLPEPQSAPRDPAATSKSSGKSAPEHLKSPSRVNKS

Target ORF information:

RefSeq Version	NM_198109.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus slingshot protein phosphatase 1 (Ssh1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198109.5

ORF Insert Sequence:

ATGGCCTTGG TGACCCTTCA GCGCTCTCCC ACGCCCAGCG CCGCGTCTTC CTCCGCAAGC 
AACAGCGAGT TGGAGGCTGG CAGCGATGAA GAACGGAAAT TGAACCTCAG CTTGAGTGAG 
AGCTTTTTCA TGGTGAAAGG AGCTGCCCTC TTCCTACAGC AGGGAAACAG CCCACAGGGC 
CAGCGGAGTC TTCAGCACCC TCACAAGCAT GCAGGTGATC TGCCTCAGCA CCTGCAAGTG 
ATGATCAACC TCCTGCGTTG TGAAGACAGA ATCAAGCTGG CCGTGCGCCT AGAGAGTGTC 
TGGACCGACC GTGTCCGCTA CATGGTCGTG GTATACACCA GCGGGCGCCA GGACACCGAG 
GAGAATATTC TGCTGGGAGT TGACTTTTCC AGTAAGGAGA GCAAAAGCTG CACGATCGGA 
ATGGTTCTTC GACTCTGGAG CGACACCAAG ATTCACCTCG ATGGGGACGG CGGGTTCAGT 
GTGAGCACAG CGGGCAGGAT GCACATATTC AAGCCAGTGT CTGTCCAGGC CATGTGGTCT 
GCCCTGCAAG TGCTTCACAA GGCCTGCGAA GTGGCCCGGA GGCATAACTA CTTCCCAGGA 
GGAGTGGCGC TCATCTGGGC CACCTACTAT GAGAGCTGCA TCAGCTCGGA GCAGAGCTGC 
ATCAATGAGT GGAATGCCAT GCAGGACCTG GAGTCCACGC GGCCCGACTC CCCCGCGCTG 
TTTGTGGACA AGCCAACCGA GGGCGAAAGA ACTGAGCGCC TCATTAAAGC CAAACTCCGG 
AGCATCATGA TGAGCCAGGA CCTTGAAAAT GTGACTTCTA AGGAAATCCG AAATGAGCTG 
GAGAAGCAAA TGAACTGCAA CCTGAAGGAG TTCAAGGAAT TCATCGATAA CGAGATGCTG 
CTCATCCTGG GCCAGATGGA CAAGCCCTCC CTCATCTTTG ACCATCTTTA TCTTGGCTCC 
GAGTGGAATG CATCCAATCT GGAGGAGCTG CAGGGCTCAG GAGTTGACTA CATTTTAAAT 
GTCACTAGAG AAATAGACAA TTTTTTCCCC GGCTTGTTTG CGTACCATAA CATCCGCGTG 
TACGATGAGG AGACCACAGA CCTTCTTGCC CACTGGAATG AAGCTTATCA TTTCATAAAC 
AAAGCGAAAA GGAATCATTC CAAGTGCCTG GTCCATTGCA AGATGGGCGT CAGCCGATCT 
GCGTCCACAG TCATTGCCTA CGCCATGAAG GAGTTCGGCT GGCCCCTGGA GAAAGCGTAT 
AACTACGTGA AGCAGAAACG TAGTATCACT CGGCCCAACG CAGGCTTTAT GAGGCAGCTG 
TCTGAGTACG AAGGCATCCT GGATGCAAGC AAACAGCGGC ATAACAAACT ATGGCGCCAG 
CAGCCCACAG ATGACACCAT CGCAGAGCCC AGTGAGTTCT TGCCAGAGAC CTTGGACGGA 
GCCCTGGACG CTCAGCTGCC CTGTTTGGAT GATACCACCC ACCCTGGGCT CCCGAGAAGT 
CTGGCCCCAG GAGGACCCGC TCTCCCCTGT TGTTTTCGAA GACTCTCGGA CCCCCTCCTC 
CTTCCCCACC ATGATGAAAC GGGTGGCCTG GTCCACTTAG AGGATCTCGA GAAGGATGCT 
CTGTTAGAGG AGGAGGAGTC TCAGCCAGTG GAGGTGCACA AGCTGGTTCA GCATCCCCAG 
GAAGGTGCCA GGCTGTGTGA AAAGGACGTA AAGAGGAAAC TGGAGTTTGG GAACTCCAAA 
CCCCGCAGTG ACTCCTTGCC TCAGGTGGAG GAGCTGGAGA AGGACGGTAG CCCAAGAACG 
GGGAGGTGGA GGCGGGCCTC CACCCAGCTC GATAGAAGCT TGCTCGACCA GGAAAACCTA 
AATAACAACA ACAGCAAGAG GAGCTGTCCC GATGACTTGG AGCGCGATGC CATGTTTGGA 
ATTCTCAGCA AAGTGAAGCC TCCCTACACA TCCTGTGCCG ACTGCATGTA CCCCACAGCT 
GGTGGGACTC CCGAGGCCTA CATGGAGCGA CACGAAGACC CCAGCTCCTC TGCTATCTGC 
ACCCAGCCAA CCTTCCTCCC CCATGTCACG TCTTCCCCAA TGGCCCACGC GAGCAGCAGG 
TCCCGAGCTC CGGAGAGGCC GGCCTCGGGT CCAGCCAACA CCTCTCCATT CCTACTACCA 
GCAGGCTCCA GGAAGCCAGA TGTCAGTGGC TCTGGAGCCG GGGCTGCCCC GGAACCACCA 
GCAAGCCTTC TAGAGCCTTC CAGAGAGACC TCAAAAGCCC TTCCAAAATC TCTCCAGTTG 
AAGAATCCTC ACTGTGACAA GAACGCTGCC AATATGGAGG TGTCGGCGAA GGAAGAACCG 
TCGCCTAAGA AAGATCCCAA GCCGGCTAAG GACCTGAGGC TTCTGTTCAG TAACGAGGCG 
GAGAAGCCCA CCACCAACAG CTACCTGATG CAGCATCAGG AGTCCATCAT CCAACTGCAG 
AAGGCAGGCC TGGTCCGAAA GCACACCAAA GAGCTGGAGA GGTTGAAGAG CCTGCCTTCA 
GACTCACCAG CTGCCTGCAG GGACAGCGCC ACCTGCAGGC TGGAGGCCAG CATCCCGGAG 
GAGGGTAGCC AGGAGCCCGC ACACCCAGCC CTGTGCAGCC AAGCTGGGTC GGAAGAACAG 
CCTGTAGGGG GAACCTTGCA GAAGAGCCCC ACGTCTACCC TCCCCCGTTT AGATCACACC 
AGTAACTTCT CGAAGGACTT CCTGAAGACC GTGTGTTACA CCCCCACCTC CTCCTCCATC 
AGCTCCAACC TGACCCGGAG CTCCAGCAGC GACAGCATCC ACAGCGTCCG AGGGAAGCCT 
GGGCTGGTGA AGCAGCGGGC GCAGGAGATC GAGACGCGGC TGCGCCTGGC AGGCCTCACC 
GTGTCGTCCC CGCTGAAAAG GTCCCATTCC CTTGCCAAGC TGGGAAGTCT CAACTTCTCC 
ACGGAGGACC TGTCCAGCGA GGCTGACACA TCCACCATCG CTGACTCGCA GGACGCCAAG 
TGTGGTCTCT CCTCTTCCTT CTTGCCCGAA CCCCAGTCTG CGCCAAGGGA CCCCGCTGCA 
ACCTCTAAAT CATCAGGGAA ATCTGCCCCA GAACACTTGA AAAGCCCGTC GAGGGTAAAC 
AAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ssh1 ( XM_011248193.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246495.1 Mus musculus slingshot homolog 1 (Drosophila) (Ssh1), transcript variant X1, mRNA.

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CloneID	OMu73571
Clone ID Related Accession (Same CDS sequence)	XM_011248193.2 , XM_011248193.3
Accession Version	XM_011248193.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein phosphatase Slingshot homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATCC TTGTTTTGCT GGCTCCAGAC TTGCTGTGTA TTGAAGGGGT GACCTTGAAC 
TCTGATCACC TGGTTTCCCT CACCCGACTG AGTGCCAGGA TTACAGCTGT GGTGTGCATC 
ACCACACCTG GCTTGTGGCG GGCCAGAGAC CTTCATGATT GCTCAACAAG CATCCTACCA 
ACCGAGCTAC ATCCCGGCCT CTGCTTGAGT GAGAGCTTTT TCATGGTGAA AGGAGCTGCC 
CTCTTCCTAC AGCAGGGAAA CAGCCCACAG GGCCAGCGGA GTCTTCAGCA CCCTCACAAG 
CATGCAGGTG ATCTGCCTCA GCACCTGCAA GTGATGATCA ACCTCCTGCG TTGTGAAGAC 
AGAATCAAGC TGGCCGTGCG CCTAGAGAGT GTCTGGACCG ACCGTGTCCG CTACATGGTC 
GTGGTATACA CCAGCGGGCG CCAGGACACC GAGGAGAATA TTCTGCTGGG AGTTGACTTT 
TCCAGTAAGG AGAGCAAAAG CTGCACGATC GGAATGGTTC TTCGACTCTG GAGCGACACC 
AAGATTCACC TCGATGGGGA CGGCGGGTTC AGTGTGAGCA CAGCGGGCAG GATGCACATA 
TTCAAGCCAG TGTCTGTCCA GGCCATGTGG TCTGCCCTGC AAGTGCTTCA CAAGGCCTGC 
GAAGTGGCCC GGAGGCATAA CTACTTCCCA GGAGGAGTGG CGCTCATCTG GGCCACCTAC 
TATGAGAGCT GCATCAGCTC GGAGCAGAGC TGCATCAATG AGTGGAATGC CATGCAGGAC 
CTGGAGTCCA CGCGGCCCGA CTCCCCCGCG CTGTTTGTGG ACAAGCCAAC CGAGGGCGAA 
AGAACTGAGC GCCTCATTAA AGCCAAACTC CGGAGCATCA TGATGAGCCA GGACCTTGAA 
AATGTGACTT CTAAGGAAAT CCGAAATGAG CTGGAGAAGC AAATGAACTG CAACCTGAAG 
GAGTTCAAGG AATTCATCGA TAACGAGATG CTGCTCATCC TGGGCCAGAT GGACAAGCCC 
TCCCTCATCT TTGACCATCT TTATCTTGGC TCCGAGTGGA ATGCATCCAA TCTGGAGGAG 
CTGCAGGGCT CAGGAGTTGA CTACATTTTA AATGTCACTA GAGAAATAGA CAATTTTTTC 
CCCGGCTTGT TTGCGTACCA TAACATCCGC GTGTACGATG AGGAGACCAC AGACCTTCTT 
GCCCACTGGA ATGAAGCTTA TCATTTCATA AACAAAGCGA AAAGGAATCA TTCCAAGTGC 
CTGGTCCATT GCAAGATGGG CGTCAGCCGA TCTGCGTCCA CAGTCATTGC CTACGCCATG 
AAGGAGTTCG GCTGGCCCCT GGAGAAAGCG TATAACTACG TGAAGCAGAA ACGTAGTATC 
ACTCGGCCCA ACGCAGGCTT TATGAGGCAG CTGTCTGAGT ACGAAGGCAT CCTGGATGCA 
AGCAAACAGC GGCATAACAA ACTATGGCGC CAGCAGCCCA CAGATGACAC CATCGCAGAG 
CCCAGTGAGT TCTTGCCAGA GACCTTGGAC GGAGCCCTGG ACGCTCAGCT GCCCTGTTTG 
GATGATACCA CCCACCCTGG GCTCCCGAGA AGTCTGGCCC CAGGAGGACC CGCTCTCCCC 
TGTTGTTTTC GAAGACTCTC GGACCCCCTC CTCCTTCCCC ACCATGATGA AACGGGTGGC 
CTGGTCCACT TAGAGGATCT CGAGAAGGAT GCTCTGTTAG AGGAGGAGGA GTCTCAGCCA 
GTGGAGGTGC ACAAGCTGGT TCAGCATCCC CAGGAAGGTG CCAGGCTGTG TGAAAAGGAC 
GTAAAGAGGA AACTGGAGTT TGGGAACTCC AAACCCCGCA GTGACTCCTT GCCTCAGGTG 
GAGGAGCTGG AGAAGGACGG TAGCCCAAGA ACGGGGAGGT GGAGGCGGGC CTCCACCCAG 
CTCGATAGAA GCTTGCTCGA CCAGGAAAAC CTAAATAACA ACAACAGCAA GAGGAGCTGT 
CCCGATGACT TGGAGCGCGA TGCCATGTTT GGAATTCTCA GCAAAGTGAA GCCTCCCTAC 
ACATCCTGTG CCGACTGCAT GTACCCCACA GCTGGTGGGA CTCCCGAGGC CTACATGGAG 
CGACACGAAG ACCCCAGCTC CTCTGCTATC TGCACCCAGC CAACCTTCCT CCCCCATGTC 
ACGTCTTCCC CAATGGCCCA CGCGAGCAGC AGGTCCCGAG CTCCGGAGAG GCCGGCCTCG 
GGTCCAGCCA ACACCTCTCC ATTCCTACTA CCAGCAGGCT CCAGGAAGCC AGATGTCAGT 
GGCTCTGGAG CCGGGGCTGC CCCGGAACCA CCAGCAAGCC TTCTAGAGCC TTCCAGAGAG 
ACCTCAAAAG CCCTTCCAAA ATCTCTCCAG TTGAAGAATC CTCACTGTGA CAAGAACGCT 
GCCAATATGG AGGTGTCGGC GAAGGAAGAA CCGTCGCCTA AGAAAGATCC CAAGCCGGCT 
AAGGACCTGA GGCTTCTGTT CAGTAACGAG GCGGAGAAGC CCACCACCAA CAGCTACCTG 
ATGCAGCATC AGGAGTCCAT CATCCAACTG CAGAAGGCAG GCCTGGTCCG AAAGCACACC 
AAAGAGCTGG AGAGGTTGAA GAGCCTGCCT TCAGACTCAC CAGCTGCCTG CAGGGACAGC 
GCCACCTGCA GGCTGGAGGC CAGCATCCCG GAGGAGGGTA GCCAGGAGCC CGCACACCCA 
GCCCTGTGCA GCCAAGCTGG GTCGGAAGAA CAGCCTGTAG GGGGAACCTT GCAGAAGAGC 
CCCACGTCTA CCCTCCCCCG TTTAGATCAC ACCAGTAACT TCTCGAAGGA CTTCCTGAAG 
ACCGTGTGTT ACACCCCCAC CTCCTCCTCC ATCAGCTCCA ACCTGACCCG GAGCTCCAGC 
AGCGACAGCA TCCACAGCGT CCGAGGGAAG CCTGGGCTGG TGAAGCAGCG GGCGCAGGAG 
ATCGAGACGC GGCTGCGCCT GGCAGGCCTC ACCGTGTCGT CCCCGCTGAA AAGGTCCCAT 
TCCCTTGCCA AGCTGGGAAG TCTCAACTTC TCCACGGAGG ACCTGTCCAG CGAGGCTGAC 
ACATCCACCA TCGCTGACTC GCAGGACGCC AAGTGTGGTC TCTCCTCTTC CTTCTTGCCC 
GAACCCCAGT CTGCGCCAAG GGACCCCGCT GCAACCTCTA AATCATCAGG GAAATCTGCC 
CCAGAACACT TGAAAAGCCC GTCGAGGGTA AACAAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246495.1
CDS	99..3320
Translation	MMILVLLAPDLLCIEGVTLNSDHLVSLTRLSARITAVVCITTPGLWRARDLHDCSTSILPTELHPGLCLSESFFMVKGAALFLQQGNSPQGQRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESVWTDRVRYMVVVYTSGRQDTEENILLGVDFSSKESKSCTIGMVLRLWSDTKIHLDGDGGFSVSTAGRMHIFKPVSVQAMWSALQVLHKACEVARRHNYFPGGVALIWATYYESCISSEQSCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEIRNELEKQMNCNLKEFKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYILNVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAMKEFGWPLEKAYNYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQPTDDTIAEPSEFLPETLDGALDAQLPCLDDTTHPGLPRSLAPGGPALPCCFRRLSDPLLLPHHDETGGLVHLEDLEKDALLEEEESQPVEVHKLVQHPQEGARLCEKDVKRKLEFGNSKPRSDSLPQVEELEKDGSPRTGRWRRASTQLDRSLLDQENLNNNNSKRSCPDDLERDAMFGILSKVKPPYTSCADCMYPTAGGTPEAYMERHEDPSSSAICTQPTFLPHVTSSPMAHASSRSRAPERPASGPANTSPFLLPAGSRKPDVSGSGAGAAPEPPASLLEPSRETSKALPKSLQLKNPHCDKNAANMEVSAKEEPSPKKDPKPAKDLRLLFSNEAEKPTTNSYLMQHQESIIQLQKAGLVRKHTKELERLKSLPSDSPAACRDSATCRLEASIPEEGSQEPAHPALCSQAGSEEQPVGGTLQKSPTSTLPRLDHTSNFSKDFLKTVCYTPTSSSISSNLTRSSSSDSIHSVRGKPGLVKQRAQEIETRLRLAGLTVSSPLKRSHSLAKLGSLNFSTEDLSSEADTSTIADSQDAKCGLSSSFLPEPQSAPRDPAATSKSSGKSAPEHLKSPSRVNKS

Target ORF information:

RefSeq Version	XM_011248193.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus slingshot homolog 1 (Drosophila) (Ssh1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248193.2

ORF Insert Sequence:

ATGATGATCC TTGTTTTGCT GGCTCCAGAC TTGCTGTGTA TTGAAGGGGT GACCTTGAAC 
TCTGATCACC TGGTTTCCCT CACCCGACTG AGTGCCAGGA TTACAGCTGT GGTGTGCATC 
ACCACACCTG GCTTGTGGCG GGCCAGAGAC CTTCATGATT GCTCAACAAG CATCCTACCA 
ACCGAGCTAC ATCCCGGCCT CTGCTTGAGT GAGAGCTTTT TCATGGTGAA AGGAGCTGCC 
CTCTTCCTAC AGCAGGGAAA CAGCCCACAG GGCCAGCGGA GTCTTCAGCA CCCTCACAAG 
CATGCAGGTG ATCTGCCTCA GCACCTGCAA GTGATGATCA ACCTCCTGCG TTGTGAAGAC 
AGAATCAAGC TGGCCGTGCG CCTAGAGAGT GTCTGGACCG ACCGTGTCCG CTACATGGTC 
GTGGTATACA CCAGCGGGCG CCAGGACACC GAGGAGAATA TTCTGCTGGG AGTTGACTTT 
TCCAGTAAGG AGAGCAAAAG CTGCACGATC GGAATGGTTC TTCGACTCTG GAGCGACACC 
AAGATTCACC TCGATGGGGA CGGCGGGTTC AGTGTGAGCA CAGCGGGCAG GATGCACATA 
TTCAAGCCAG TGTCTGTCCA GGCCATGTGG TCTGCCCTGC AAGTGCTTCA CAAGGCCTGC 
GAAGTGGCCC GGAGGCATAA CTACTTCCCA GGAGGAGTGG CGCTCATCTG GGCCACCTAC 
TATGAGAGCT GCATCAGCTC GGAGCAGAGC TGCATCAATG AGTGGAATGC CATGCAGGAC 
CTGGAGTCCA CGCGGCCCGA CTCCCCCGCG CTGTTTGTGG ACAAGCCAAC CGAGGGCGAA 
AGAACTGAGC GCCTCATTAA AGCCAAACTC CGGAGCATCA TGATGAGCCA GGACCTTGAA 
AATGTGACTT CTAAGGAAAT CCGAAATGAG CTGGAGAAGC AAATGAACTG CAACCTGAAG 
GAGTTCAAGG AATTCATCGA TAACGAGATG CTGCTCATCC TGGGCCAGAT GGACAAGCCC 
TCCCTCATCT TTGACCATCT TTATCTTGGC TCCGAGTGGA ATGCATCCAA TCTGGAGGAG 
CTGCAGGGCT CAGGAGTTGA CTACATTTTA AATGTCACTA GAGAAATAGA CAATTTTTTC 
CCCGGCTTGT TTGCGTACCA TAACATCCGC GTGTACGATG AGGAGACCAC AGACCTTCTT 
GCCCACTGGA ATGAAGCTTA TCATTTCATA AACAAAGCGA AAAGGAATCA TTCCAAGTGC 
CTGGTCCATT GCAAGATGGG CGTCAGCCGA TCTGCGTCCA CAGTCATTGC CTACGCCATG 
AAGGAGTTCG GCTGGCCCCT GGAGAAAGCG TATAACTACG TGAAGCAGAA ACGTAGTATC 
ACTCGGCCCA ACGCAGGCTT TATGAGGCAG CTGTCTGAGT ACGAAGGCAT CCTGGATGCA 
AGCAAACAGC GGCATAACAA ACTATGGCGC CAGCAGCCCA CAGATGACAC CATCGCAGAG 
CCCAGTGAGT TCTTGCCAGA GACCTTGGAC GGAGCCCTGG ACGCTCAGCT GCCCTGTTTG 
GATGATACCA CCCACCCTGG GCTCCCGAGA AGTCTGGCCC CAGGAGGACC CGCTCTCCCC 
TGTTGTTTTC GAAGACTCTC GGACCCCCTC CTCCTTCCCC ACCATGATGA AACGGGTGGC 
CTGGTCCACT TAGAGGATCT CGAGAAGGAT GCTCTGTTAG AGGAGGAGGA GTCTCAGCCA 
GTGGAGGTGC ACAAGCTGGT TCAGCATCCC CAGGAAGGTG CCAGGCTGTG TGAAAAGGAC 
GTAAAGAGGA AACTGGAGTT TGGGAACTCC AAACCCCGCA GTGACTCCTT GCCTCAGGTG 
GAGGAGCTGG AGAAGGACGG TAGCCCAAGA ACGGGGAGGT GGAGGCGGGC CTCCACCCAG 
CTCGATAGAA GCTTGCTCGA CCAGGAAAAC CTAAATAACA ACAACAGCAA GAGGAGCTGT 
CCCGATGACT TGGAGCGCGA TGCCATGTTT GGAATTCTCA GCAAAGTGAA GCCTCCCTAC 
ACATCCTGTG CCGACTGCAT GTACCCCACA GCTGGTGGGA CTCCCGAGGC CTACATGGAG 
CGACACGAAG ACCCCAGCTC CTCTGCTATC TGCACCCAGC CAACCTTCCT CCCCCATGTC 
ACGTCTTCCC CAATGGCCCA CGCGAGCAGC AGGTCCCGAG CTCCGGAGAG GCCGGCCTCG 
GGTCCAGCCA ACACCTCTCC ATTCCTACTA CCAGCAGGCT CCAGGAAGCC AGATGTCAGT 
GGCTCTGGAG CCGGGGCTGC CCCGGAACCA CCAGCAAGCC TTCTAGAGCC TTCCAGAGAG 
ACCTCAAAAG CCCTTCCAAA ATCTCTCCAG TTGAAGAATC CTCACTGTGA CAAGAACGCT 
GCCAATATGG AGGTGTCGGC GAAGGAAGAA CCGTCGCCTA AGAAAGATCC CAAGCCGGCT 
AAGGACCTGA GGCTTCTGTT CAGTAACGAG GCGGAGAAGC CCACCACCAA CAGCTACCTG 
ATGCAGCATC AGGAGTCCAT CATCCAACTG CAGAAGGCAG GCCTGGTCCG AAAGCACACC 
AAAGAGCTGG AGAGGTTGAA GAGCCTGCCT TCAGACTCAC CAGCTGCCTG CAGGGACAGC 
GCCACCTGCA GGCTGGAGGC CAGCATCCCG GAGGAGGGTA GCCAGGAGCC CGCACACCCA 
GCCCTGTGCA GCCAAGCTGG GTCGGAAGAA CAGCCTGTAG GGGGAACCTT GCAGAAGAGC 
CCCACGTCTA CCCTCCCCCG TTTAGATCAC ACCAGTAACT TCTCGAAGGA CTTCCTGAAG 
ACCGTGTGTT ACACCCCCAC CTCCTCCTCC ATCAGCTCCA ACCTGACCCG GAGCTCCAGC 
AGCGACAGCA TCCACAGCGT CCGAGGGAAG CCTGGGCTGG TGAAGCAGCG GGCGCAGGAG 
ATCGAGACGC GGCTGCGCCT GGCAGGCCTC ACCGTGTCGT CCCCGCTGAA AAGGTCCCAT 
TCCCTTGCCA AGCTGGGAAG TCTCAACTTC TCCACGGAGG ACCTGTCCAG CGAGGCTGAC 
ACATCCACCA TCGCTGACTC GCAGGACGCC AAGTGTGGTC TCTCCTCTTC CTTCTTGCCC 
GAACCCCAGT CTGCGCCAAG GGACCCCGCT GCAACCTCTA AATCATCAGG GAAATCTGCC 
CCAGAACACT TGAAAAGCCC GTCGAGGGTA AACAAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Caspase-11 and caspase-1 differentially modulate actin polymerization via RhoA and Slingshot proteins to promote bacterial clearance.
Scientific reports518479(2015 Dec)
Caution K,Gavrilin MA,Tazi M,Kanneganti A,Layman D,Hoque S,Krause K,Amer AO

High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes.
Bone research214034(2014)
Brommage R,Liu J,Hansen GM,Kirkpatrick LL,Potter DG,Sands AT,Zambrowicz B,Powell DR,Vogel P

PLC?, PKD1, and SSH1L transduce RhoA signaling to protect mitochondria from oxidative stress in the heart.
Science signaling6(306)ra108(2013 Dec)
Xiang SY,Ouyang K,Yung BS,Miyamoto S,Smrcka AV,Chen J,Heller Brown J

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Ssh1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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