Ece1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ece1
Entrez Gene ID	230857
Full Name	endothelin converting enzyme 1
Synonyms	AW322500,ECE-1,ECE-1a,ECE-1b,ECE-1d
General protein information	Preferred Names endothelin converting enzyme 1 Names endothelin-converting enzyme 1 endothelin-converting enzyme-1a endothelin-converting enzyme-1b endothelin-converting enzyme-1d
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006538765.1	XP_006538828.1	endothelin-converting enzyme 1 isoform X1
XM_006538767.3	XP_006538830.1	endothelin-converting enzyme 1 isoform X2
XM_006538767.2	XP_006538830.1	endothelin-converting enzyme 1 isoform X1
NM_001369179.1	NP_001356108.1	endothelin-converting enzyme 1 isoform c
NM_001369178.1	NP_001356107.1	endothelin-converting enzyme 1 isoform b
NM_001369177.1	NP_001356106.1	endothelin-converting enzyme 1 isoform a
NM_199307.2	NP_955011.1	endothelin-converting enzyme 1 isoform c

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ECE1	XP_001162780.1
Mus musculus (house mouse)	Ece1	NP_955011.1
Bos taurus (cattle)	ECE1	NP_851352.1
Rattus norvegicus (Norway rat)	Ece1	NP_446048.2
Danio rerio (zebrafish)	ece1	NP_001071260.1
	ECE1	XP_001099089.1
Gallus gallus (chicken)	ECE1	NP_990048.1
Canis lupus familiaris (dog)	ECE1	XP_544514.3
Drosophila melanogaster (fruit fly)	Nep3	NP_001245776.1
Homo sapiens (human)	ECE1	NP_001106820.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007796	XP_317711.1

Gene Ontology
Bibliography

Related articles in PubMed

Spontaneous recombinase activity of Cre-ERT2 in vivo.
Kristianto J, Johnson MG, Zastrow RK, Radcliff AB, Blank RD
Transgenic research26(3)411-417(2017 Jun)

Nkx2.5 regulates endothelin converting enzyme-1 during pharyngeal arch patterning.
Ikl? JM, Tavares AL, King M, Ding H, Colombo S, Firulli BA, Firulli AB, Targoff KL, Yelon D, Clouthier DE
Genesis (New York, N.Y. : 2000)55(3)(2017 Mar)

Major amyloid-?-degrading enzymes, endothelin-converting enzyme-2 and neprilysin, are expressed by distinct populations of GABAergic interneurons in hippocampus and neocortex.
Pacheco-Quinto J, Eckman CB, Eckman EA
Neurobiology of aging4883-92(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It is a amyloid beta degrading enzyme and is expressed by distinct populations of GABAergic interneurons in hippocampus and neocortex.
Title: Major amyloid-?-degrading enzymes, endothelin-converting enzyme-2 and neprilysin, are expressed by distinct populations of GABAergic interneurons in hippocampus and neocortex.

The cardiac expression of prepro-endothelin-1 mRNA, endothelin-converting enzyme-1 mRNA, and protein and endothelin receptors A and B mRNA was increased in 18-week-old obese C57BL/6 mice compared to animals with normal weight
Title: Increased gene expression of the cardiac endothelin system in obese mice.

identify the neural peptidase ECE-1 as a negative regulator of itch on sensory nerves by directly regulating ET-1-induced pruritus
Title: Neural peptidase endothelin-converting enzyme 1 regulates endothelin 1-induced pruritus.

differences in hemodynamic adn ece1 expression between HcB-8 and HcB-23 arteries
Title: Blood pressure, artery size, and artery compliance parallel bone size and strength in mice with differing ece1 expression.

Endothelin-converting enzyme-1 gene ablation attenuates pulmonary fibrosis via CGRP-cAMP/EPAC1 pathway.
Title: Endothelin-converting enzyme-1 gene ablation attenuates pulmonary fibrosis via CGRP-cAMP/EPAC1 pathway.

The following Ece1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ece1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu34125	NM_001369177.1 Latest version!	Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu34125	XM_006538765.1 Latest version!	Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu09434	NM_001369179.1 Latest version!	Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu09434	NM_199307.2 Latest version!	Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu84948	NM_001369178.1 Latest version!	Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu09434	XM_006538767.3 Latest version!	Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
View Old Accession Versions >>
OMu09434	XM_006538767.2	Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ece1 ( NM_001369177.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001356106.1 Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu34125
Clone ID Related Accession (Same CDS sequence)	XM_006538765.1 , NM_001369177.1
Accession Version	NM_001369177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	endothelin-converting enzyme 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL807764.9. On Mar 22, 2019 this sequence version replaced XM_006538765.1. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.1452805.1, SRR5314776.72352.5 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCGGACCG TGTGGTCGCC GCTGGCCGCC GCGCTGGCCG CTCTGGGGAT GTCAACCTAC 
AAGCGGGCCA CGCTGGACGA AGAGGATCTG GTGGACTCAC TCTCCGAGGG CGATGTGTAC 
CCCAATGGCC TACAGGTGAA CTTCCGCAGC TCCCGGAGCG GCCAGAGGTG CTGGGCAGCT 
CGGACCTCGG TGGAGAAGCG GCTGGTGGTG CTGGTGACGC TTCTGGCGGC AGGGTTGGTG 
GCCTGCCTGG CAGCCCTGGG CATCCAGTAC CAGACAAGAA CGCCTCCGGT ATGTCTGACG 
GAGGCCTGTG TCTCAGTGAC CAGCTCTATC CTAAACTCCA TGGACCCCAC AGTAGACCCG 
TGCCAGGACT TCTTCAGCTA CGCCTGCGGG GGCTGGATCA AGGCCAACCC CGTGCCCGAT 
GGCCACTCAC GCTGGGGAAC CTTCAGCAAC CTCTGGGAAC ACAACCAAGC TGTCATCAAG 
CATCTACTGG AAAATGCCAC GGCCAGTGTG AGCGAGGCAG AGAGAAAAGC CCAAGTGTAC 
TACCGTGCGT GCATGAACGA AACTAGGATT GAGGAGCTTC GGGCCAAGCC CCTGATGGAG 
CTGATTGAGA AGCTGGGAGG CTGGAATATC ACAGGACCCT GGGCCAAGGA CAACTTCCAG 
GACACACTGC AGGTGGTCAC AGCTCACTAC CGCACCTCAC CCTTCTTCTC TGTTTATGTC 
AGCGCGGACT CCAAGAATTC CAACAGCAAT GTGATCCAGG TGGACCAGTC CGGCCTTGGC 
TTGCCCTCCA GAGACTATTA CCTGAACAAG ACAGAGAATG AAAAGGTCCT GACTGGATAC 
CTGAACTACA TGGTCCAGCT GGGGAAGCTC CTGGGCGGTG GGGACGAGGA TGCCATCCGG 
CCACAGATGC AGCAGATCCT GGATTTTGAG ACGGCGCTGG CCAACATCAC CATCCCCCAG 
GAGAAGCGCA GGGATGAAGA ACTCATCTAC CACAAAGTCA CGGCTGCCGA GCTGCAGACC 
TTGGCACCTG CCATCAACTG GTTACCCTTT CTGAACACCA TTTTTTACCC AGTGGAGATC 
AATGAGTCTG AGCCAATCGT GGTCTACGAC AAGGAATACT TGAGACAAGT CTCCACCCTC 
ATCAACAACA CCGACAAATG TCTGCTCAAC AACTACATGA TGTGGAACCT GGTACGCAAA 
ACAAGCTCCT TCCTGGACCA GCGCTTTCAG GACGCCGACG AGAAGTTCAT GGAGGTTATG 
TATGGGACGA AGAAGACCTG TATTCCCCGC TGGAAGTTTT GTGTGAGTGA CACGGAAAAC 
AACCTGGGCT TTGCCCTGGG ACCCATGTTT GTGAAAGCAA CCTTTGCAGA AGACAGTAAG 
AATATAGCCA GTGAGATCAT CATGGAGATC AAGAAGGCGT TTGAGGAGAG TCTGAGCACT 
CTGAAATGGA TGGATGAAGA GACTCGGAGA TCAGCCAAGG AGAAGGCGGA TGCTATCTAC 
AACATGATAG GCTACCCCAA CTTCATCATG GACCCCAAGG AGCTGGACAA AGTCTTCAAT 
GACTACACAG CAGTTCCTGA TCTCTACTTT GAGAACGCCA TGCGCTTTTT CAACTTCTCA 
TGGAGGGTCA CAGCTGACCA GCTCAGAAAA GCTCCCAACA GAGATCAGTG GAGTATGACG 
CCGCCCATGG TGAACGCTTA CTACTCGCCC ACCAAGAACG AGATCGTGTT TCCAGCTGGA 
ATACTGCAGG CGCCATTTTA TACCCGCTCT TCACCCAACG CCTTGAACTT CGGTGGCATT 
GGTGTCGTAG TGGGCCACGA GCTGACTCAC GCTTTCGATG ATCAAGGTCG GGAGTACGAC 
AAGGATGGGA ACCTTCGACC CTGGTGGAAG AACTCGTCCG TGGAGGCGTT CAAGCAGCAG 
ACGGAGTGCA TGGTGCAGCA ATACAGCAAC TACAGCGTGA ACGGGGAGCC CGTGAACGGT 
CGACACACCC TCGGGGAGAA CATTGCCGAC AACGGGGGAC TCAAGGCGGC CTACCGGGCG 
TACCAGAACT GGGTAAAGAA GAACGGAGCT GAGCAGACGC TGCCCACCCT GGGTCTCACC 
AGCAACCAGC TCTTCTTCCT AGGATTTGCA CAGGTCTGGT GCTCTGTCCG CACACCTGAG 
AGCTCCCACG AAGGCCTCAT CACGGATCCG CACAGCCCCT CTCGCTTCCG GGTCATCGGC 
TCGCTCTCCA ACTCCAAGGA GTTCTCAGAA CACTTTCGCT GCCCGCCTGG CTCTCCCATG 
AACCCTCATC ACAAGTGCGA AGTCTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356106.1
CDS	25..2334
Translation	MRTVWSPLAAALAALGMSTYKRATLDEEDLVDSLSEGDVYPNGLQVNFRSSRSGQRCWAARTSVEKRLVVLVTLLAAGLVACLAALGIQYQTRTPPVCLTEACVSVTSSILNSMDPTVDPCQDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAVIKHLLENATASVSEAERKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEDAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLRQVSTLINNTDKCLLNNYMMWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCIPRWKFCVSDTENNLGFALGPMFVKATFAEDSKNIASEIIMEIKKAFEESLSTLKWMDEETRRSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVQQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTSNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	NM_001369177.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369177.1

ORF Insert Sequence:

ATGCGGACCG TGTGGTCGCC GCTGGCCGCC GCGCTGGCCG CTCTGGGGAT GTCAACCTAC 
AAGCGGGCCA CGCTGGACGA AGAGGATCTG GTGGACTCAC TCTCCGAGGG CGATGTGTAC 
CCCAATGGCC TACAGGTGAA CTTCCGCAGC TCCCGGAGCG GCCAGAGGTG CTGGGCAGCT 
CGGACCTCGG TGGAGAAGCG GCTGGTGGTG CTGGTGACGC TTCTGGCGGC AGGGTTGGTG 
GCCTGCCTGG CAGCCCTGGG CATCCAGTAC CAGACAAGAA CGCCTCCGGT ATGTCTGACG 
GAGGCCTGTG TCTCAGTGAC CAGCTCTATC CTAAACTCCA TGGACCCCAC AGTAGACCCG 
TGCCAGGACT TCTTCAGCTA CGCCTGCGGG GGCTGGATCA AGGCCAACCC CGTGCCCGAT 
GGCCACTCAC GCTGGGGAAC CTTCAGCAAC CTCTGGGAAC ACAACCAAGC TGTCATCAAG 
CATCTACTGG AAAATGCCAC GGCCAGTGTG AGCGAGGCAG AGAGAAAAGC CCAAGTGTAC 
TACCGTGCGT GCATGAACGA AACTAGGATT GAGGAGCTTC GGGCCAAGCC CCTGATGGAG 
CTGATTGAGA AGCTGGGAGG CTGGAATATC ACAGGACCCT GGGCCAAGGA CAACTTCCAG 
GACACACTGC AGGTGGTCAC AGCTCACTAC CGCACCTCAC CCTTCTTCTC TGTTTATGTC 
AGCGCGGACT CCAAGAATTC CAACAGCAAT GTGATCCAGG TGGACCAGTC CGGCCTTGGC 
TTGCCCTCCA GAGACTATTA CCTGAACAAG ACAGAGAATG AAAAGGTCCT GACTGGATAC 
CTGAACTACA TGGTCCAGCT GGGGAAGCTC CTGGGCGGTG GGGACGAGGA TGCCATCCGG 
CCACAGATGC AGCAGATCCT GGATTTTGAG ACGGCGCTGG CCAACATCAC CATCCCCCAG 
GAGAAGCGCA GGGATGAAGA ACTCATCTAC CACAAAGTCA CGGCTGCCGA GCTGCAGACC 
TTGGCACCTG CCATCAACTG GTTACCCTTT CTGAACACCA TTTTTTACCC AGTGGAGATC 
AATGAGTCTG AGCCAATCGT GGTCTACGAC AAGGAATACT TGAGACAAGT CTCCACCCTC 
ATCAACAACA CCGACAAATG TCTGCTCAAC AACTACATGA TGTGGAACCT GGTACGCAAA 
ACAAGCTCCT TCCTGGACCA GCGCTTTCAG GACGCCGACG AGAAGTTCAT GGAGGTTATG 
TATGGGACGA AGAAGACCTG TATTCCCCGC TGGAAGTTTT GTGTGAGTGA CACGGAAAAC 
AACCTGGGCT TTGCCCTGGG ACCCATGTTT GTGAAAGCAA CCTTTGCAGA AGACAGTAAG 
AATATAGCCA GTGAGATCAT CATGGAGATC AAGAAGGCGT TTGAGGAGAG TCTGAGCACT 
CTGAAATGGA TGGATGAAGA GACTCGGAGA TCAGCCAAGG AGAAGGCGGA TGCTATCTAC 
AACATGATAG GCTACCCCAA CTTCATCATG GACCCCAAGG AGCTGGACAA AGTCTTCAAT 
GACTACACAG CAGTTCCTGA TCTCTACTTT GAGAACGCCA TGCGCTTTTT CAACTTCTCA 
TGGAGGGTCA CAGCTGACCA GCTCAGAAAA GCTCCCAACA GAGATCAGTG GAGTATGACG 
CCGCCCATGG TGAACGCTTA CTACTCGCCC ACCAAGAACG AGATCGTGTT TCCAGCTGGA 
ATACTGCAGG CGCCATTTTA TACCCGCTCT TCACCCAACG CCTTGAACTT CGGTGGCATT 
GGTGTCGTAG TGGGCCACGA GCTGACTCAC GCTTTCGATG ATCAAGGTCG GGAGTACGAC 
AAGGATGGGA ACCTTCGACC CTGGTGGAAG AACTCGTCCG TGGAGGCGTT CAAGCAGCAG 
ACGGAGTGCA TGGTGCAGCA ATACAGCAAC TACAGCGTGA ACGGGGAGCC CGTGAACGGT 
CGACACACCC TCGGGGAGAA CATTGCCGAC AACGGGGGAC TCAAGGCGGC CTACCGGGCG 
TACCAGAACT GGGTAAAGAA GAACGGAGCT GAGCAGACGC TGCCCACCCT GGGTCTCACC 
AGCAACCAGC TCTTCTTCCT AGGATTTGCA CAGGTCTGGT GCTCTGTCCG CACACCTGAG 
AGCTCCCACG AAGGCCTCAT CACGGATCCG CACAGCCCCT CTCGCTTCCG GGTCATCGGC 
TCGCTCTCCA ACTCCAAGGA GTTCTCAGAA CACTTTCGCT GCCCGCCTGG CTCTCCCATG 
AACCCTCATC ACAAGTGCGA AGTCTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ece1 ( XM_006538765.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538828.1 Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34125
Clone ID Related Accession (Same CDS sequence)	XM_006538765.1 , NM_001369177.1
Accession Version	XM_006538765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	endothelin-converting enzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGACCG TGTGGTCGCC GCTGGCCGCC GCGCTGGCCG CTCTGGGGAT GTCAACCTAC 
AAGCGGGCCA CGCTGGACGA AGAGGATCTG GTGGACTCAC TCTCCGAGGG CGATGTGTAC 
CCCAATGGCC TACAGGTGAA CTTCCGCAGC TCCCGGAGCG GCCAGAGGTG CTGGGCAGCT 
CGGACCTCGG TGGAGAAGCG GCTGGTGGTG CTGGTGACGC TTCTGGCGGC AGGGTTGGTG 
GCCTGCCTGG CAGCCCTGGG CATCCAGTAC CAGACAAGAA CGCCTCCGGT ATGTCTGACG 
GAGGCCTGTG TCTCAGTGAC CAGCTCTATC CTAAACTCCA TGGACCCCAC AGTAGACCCG 
TGCCAGGACT TCTTCAGCTA CGCCTGCGGG GGCTGGATCA AGGCCAACCC CGTGCCCGAT 
GGCCACTCAC GCTGGGGAAC CTTCAGCAAC CTCTGGGAAC ACAACCAAGC TGTCATCAAG 
CATCTACTGG AAAATGCCAC GGCCAGTGTG AGCGAGGCAG AGAGAAAAGC CCAAGTGTAC 
TACCGTGCGT GCATGAACGA AACTAGGATT GAGGAGCTTC GGGCCAAGCC CCTGATGGAG 
CTGATTGAGA AGCTGGGAGG CTGGAATATC ACAGGACCCT GGGCCAAGGA CAACTTCCAG 
GACACACTGC AGGTGGTCAC AGCTCACTAC CGCACCTCAC CCTTCTTCTC TGTTTATGTC 
AGCGCGGACT CCAAGAATTC CAACAGCAAT GTGATCCAGG TGGACCAGTC CGGCCTTGGC 
TTGCCCTCCA GAGACTATTA CCTGAACAAG ACAGAGAATG AAAAGGTCCT GACTGGATAC 
CTGAACTACA TGGTCCAGCT GGGGAAGCTC CTGGGCGGTG GGGACGAGGA TGCCATCCGG 
CCACAGATGC AGCAGATCCT GGATTTTGAG ACGGCGCTGG CCAACATCAC CATCCCCCAG 
GAGAAGCGCA GGGATGAAGA ACTCATCTAC CACAAAGTCA CGGCTGCCGA GCTGCAGACC 
TTGGCACCTG CCATCAACTG GTTACCCTTT CTGAACACCA TTTTTTACCC AGTGGAGATC 
AATGAGTCTG AGCCAATCGT GGTCTACGAC AAGGAATACT TGAGACAAGT CTCCACCCTC 
ATCAACAACA CCGACAAATG TCTGCTCAAC AACTACATGA TGTGGAACCT GGTACGCAAA 
ACAAGCTCCT TCCTGGACCA GCGCTTTCAG GACGCCGACG AGAAGTTCAT GGAGGTTATG 
TATGGGACGA AGAAGACCTG TATTCCCCGC TGGAAGTTTT GTGTGAGTGA CACGGAAAAC 
AACCTGGGCT TTGCCCTGGG ACCCATGTTT GTGAAAGCAA CCTTTGCAGA AGACAGTAAG 
AATATAGCCA GTGAGATCAT CATGGAGATC AAGAAGGCGT TTGAGGAGAG TCTGAGCACT 
CTGAAATGGA TGGATGAAGA GACTCGGAGA TCAGCCAAGG AGAAGGCGGA TGCTATCTAC 
AACATGATAG GCTACCCCAA CTTCATCATG GACCCCAAGG AGCTGGACAA AGTCTTCAAT 
GACTACACAG CAGTTCCTGA TCTCTACTTT GAGAACGCCA TGCGCTTTTT CAACTTCTCA 
TGGAGGGTCA CAGCTGACCA GCTCAGAAAA GCTCCCAACA GAGATCAGTG GAGTATGACG 
CCGCCCATGG TGAACGCTTA CTACTCGCCC ACCAAGAACG AGATCGTGTT TCCAGCTGGA 
ATACTGCAGG CGCCATTTTA TACCCGCTCT TCACCCAACG CCTTGAACTT CGGTGGCATT 
GGTGTCGTAG TGGGCCACGA GCTGACTCAC GCTTTCGATG ATCAAGGTCG GGAGTACGAC 
AAGGATGGGA ACCTTCGACC CTGGTGGAAG AACTCGTCCG TGGAGGCGTT CAAGCAGCAG 
ACGGAGTGCA TGGTGCAGCA ATACAGCAAC TACAGCGTGA ACGGGGAGCC CGTGAACGGT 
CGACACACCC TCGGGGAGAA CATTGCCGAC AACGGGGGAC TCAAGGCGGC CTACCGGGCG 
TACCAGAACT GGGTAAAGAA GAACGGAGCT GAGCAGACGC TGCCCACCCT GGGTCTCACC 
AGCAACCAGC TCTTCTTCCT AGGATTTGCA CAGGTCTGGT GCTCTGTCCG CACACCTGAG 
AGCTCCCACG AAGGCCTCAT CACGGATCCG CACAGCCCCT CTCGCTTCCG GGTCATCGGC 
TCGCTCTCCA ACTCCAAGGA GTTCTCAGAA CACTTTCGCT GCCCGCCTGG CTCTCCCATG 
AACCCTCATC ACAAGTGCGA AGTCTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538828.1
CDS	107..2416
Translation	MRTVWSPLAAALAALGMSTYKRATLDEEDLVDSLSEGDVYPNGLQVNFRSSRSGQRCWAARTSVEKRLVVLVTLLAAGLVACLAALGIQYQTRTPPVCLTEACVSVTSSILNSMDPTVDPCQDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAVIKHLLENATASVSEAERKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEDAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLRQVSTLINNTDKCLLNNYMMWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCIPRWKFCVSDTENNLGFALGPMFVKATFAEDSKNIASEIIMEIKKAFEESLSTLKWMDEETRRSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVQQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTSNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	XM_006538765.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538765.1

ORF Insert Sequence:

ATGCGGACCG TGTGGTCGCC GCTGGCCGCC GCGCTGGCCG CTCTGGGGAT GTCAACCTAC 
AAGCGGGCCA CGCTGGACGA AGAGGATCTG GTGGACTCAC TCTCCGAGGG CGATGTGTAC 
CCCAATGGCC TACAGGTGAA CTTCCGCAGC TCCCGGAGCG GCCAGAGGTG CTGGGCAGCT 
CGGACCTCGG TGGAGAAGCG GCTGGTGGTG CTGGTGACGC TTCTGGCGGC AGGGTTGGTG 
GCCTGCCTGG CAGCCCTGGG CATCCAGTAC CAGACAAGAA CGCCTCCGGT ATGTCTGACG 
GAGGCCTGTG TCTCAGTGAC CAGCTCTATC CTAAACTCCA TGGACCCCAC AGTAGACCCG 
TGCCAGGACT TCTTCAGCTA CGCCTGCGGG GGCTGGATCA AGGCCAACCC CGTGCCCGAT 
GGCCACTCAC GCTGGGGAAC CTTCAGCAAC CTCTGGGAAC ACAACCAAGC TGTCATCAAG 
CATCTACTGG AAAATGCCAC GGCCAGTGTG AGCGAGGCAG AGAGAAAAGC CCAAGTGTAC 
TACCGTGCGT GCATGAACGA AACTAGGATT GAGGAGCTTC GGGCCAAGCC CCTGATGGAG 
CTGATTGAGA AGCTGGGAGG CTGGAATATC ACAGGACCCT GGGCCAAGGA CAACTTCCAG 
GACACACTGC AGGTGGTCAC AGCTCACTAC CGCACCTCAC CCTTCTTCTC TGTTTATGTC 
AGCGCGGACT CCAAGAATTC CAACAGCAAT GTGATCCAGG TGGACCAGTC CGGCCTTGGC 
TTGCCCTCCA GAGACTATTA CCTGAACAAG ACAGAGAATG AAAAGGTCCT GACTGGATAC 
CTGAACTACA TGGTCCAGCT GGGGAAGCTC CTGGGCGGTG GGGACGAGGA TGCCATCCGG 
CCACAGATGC AGCAGATCCT GGATTTTGAG ACGGCGCTGG CCAACATCAC CATCCCCCAG 
GAGAAGCGCA GGGATGAAGA ACTCATCTAC CACAAAGTCA CGGCTGCCGA GCTGCAGACC 
TTGGCACCTG CCATCAACTG GTTACCCTTT CTGAACACCA TTTTTTACCC AGTGGAGATC 
AATGAGTCTG AGCCAATCGT GGTCTACGAC AAGGAATACT TGAGACAAGT CTCCACCCTC 
ATCAACAACA CCGACAAATG TCTGCTCAAC AACTACATGA TGTGGAACCT GGTACGCAAA 
ACAAGCTCCT TCCTGGACCA GCGCTTTCAG GACGCCGACG AGAAGTTCAT GGAGGTTATG 
TATGGGACGA AGAAGACCTG TATTCCCCGC TGGAAGTTTT GTGTGAGTGA CACGGAAAAC 
AACCTGGGCT TTGCCCTGGG ACCCATGTTT GTGAAAGCAA CCTTTGCAGA AGACAGTAAG 
AATATAGCCA GTGAGATCAT CATGGAGATC AAGAAGGCGT TTGAGGAGAG TCTGAGCACT 
CTGAAATGGA TGGATGAAGA GACTCGGAGA TCAGCCAAGG AGAAGGCGGA TGCTATCTAC 
AACATGATAG GCTACCCCAA CTTCATCATG GACCCCAAGG AGCTGGACAA AGTCTTCAAT 
GACTACACAG CAGTTCCTGA TCTCTACTTT GAGAACGCCA TGCGCTTTTT CAACTTCTCA 
TGGAGGGTCA CAGCTGACCA GCTCAGAAAA GCTCCCAACA GAGATCAGTG GAGTATGACG 
CCGCCCATGG TGAACGCTTA CTACTCGCCC ACCAAGAACG AGATCGTGTT TCCAGCTGGA 
ATACTGCAGG CGCCATTTTA TACCCGCTCT TCACCCAACG CCTTGAACTT CGGTGGCATT 
GGTGTCGTAG TGGGCCACGA GCTGACTCAC GCTTTCGATG ATCAAGGTCG GGAGTACGAC 
AAGGATGGGA ACCTTCGACC CTGGTGGAAG AACTCGTCCG TGGAGGCGTT CAAGCAGCAG 
ACGGAGTGCA TGGTGCAGCA ATACAGCAAC TACAGCGTGA ACGGGGAGCC CGTGAACGGT 
CGACACACCC TCGGGGAGAA CATTGCCGAC AACGGGGGAC TCAAGGCGGC CTACCGGGCG 
TACCAGAACT GGGTAAAGAA GAACGGAGCT GAGCAGACGC TGCCCACCCT GGGTCTCACC 
AGCAACCAGC TCTTCTTCCT AGGATTTGCA CAGGTCTGGT GCTCTGTCCG CACACCTGAG 
AGCTCCCACG AAGGCCTCAT CACGGATCCG CACAGCCCCT CTCGCTTCCG GGTCATCGGC 
TCGCTCTCCA ACTCCAAGGA GTTCTCAGAA CACTTTCGCT GCCCGCCTGG CTCTCCCATG 
AACCCTCATC ACAAGTGCGA AGTCTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ece1 ( NM_001369179.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001356108.1 Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant 4, mRNA.

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CloneID	OMu09434
Clone ID Related Accession (Same CDS sequence)	XM_006538767.3 , XM_006538767.2 , NM_001369179.1 , NM_199307.2
Accession Version	NM_001369179.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	endothelin-converting enzyme 1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL807764.9. On Mar 22, 2019 this sequence version replaced XM_006538767.3. Transcript Variant: This variant (4), as well as variant 3, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5286961.75690.1, SRR7652917.588378.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCAACCT ACAAGCGGGC CACGCTGGAC GAAGAGGATC TGGTGGACTC ACTCTCCGAG 
GGCGATGTGT ACCCCAATGG CCTACAGGTG AACTTCCGCA GCTCCCGGAG CGGCCAGAGG 
TGCTGGGCAG CTCGGACCTC GGTGGAGAAG CGGCTGGTGG TGCTGGTGAC GCTTCTGGCG 
GCAGGGTTGG TGGCCTGCCT GGCAGCCCTG GGCATCCAGT ACCAGACAAG AACGCCTCCG 
GTATGTCTGA CGGAGGCCTG TGTCTCAGTG ACCAGCTCTA TCCTAAACTC CATGGACCCC 
ACAGTAGACC CGTGCCAGGA CTTCTTCAGC TACGCCTGCG GGGGCTGGAT CAAGGCCAAC 
CCCGTGCCCG ATGGCCACTC ACGCTGGGGA ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCTGTCATCA AGCATCTACT GGAAAATGCC ACGGCCAGTG TGAGCGAGGC AGAGAGAAAA 
GCCCAAGTGT ACTACCGTGC GTGCATGAAC GAAACTAGGA TTGAGGAGCT TCGGGCCAAG 
CCCCTGATGG AGCTGATTGA GAAGCTGGGA GGCTGGAATA TCACAGGACC CTGGGCCAAG 
GACAACTTCC AGGACACACT GCAGGTGGTC ACAGCTCACT ACCGCACCTC ACCCTTCTTC 
TCTGTTTATG TCAGCGCGGA CTCCAAGAAT TCCAACAGCA ATGTGATCCA GGTGGACCAG 
TCCGGCCTTG GCTTGCCCTC CAGAGACTAT TACCTGAACA AGACAGAGAA TGAAAAGGTC 
CTGACTGGAT ACCTGAACTA CATGGTCCAG CTGGGGAAGC TCCTGGGCGG TGGGGACGAG 
GATGCCATCC GGCCACAGAT GCAGCAGATC CTGGATTTTG AGACGGCGCT GGCCAACATC 
ACCATCCCCC AGGAGAAGCG CAGGGATGAA GAACTCATCT ACCACAAAGT CACGGCTGCC 
GAGCTGCAGA CCTTGGCACC TGCCATCAAC TGGTTACCCT TTCTGAACAC CATTTTTTAC 
CCAGTGGAGA TCAATGAGTC TGAGCCAATC GTGGTCTACG ACAAGGAATA CTTGAGACAA 
GTCTCCACCC TCATCAACAA CACCGACAAA TGTCTGCTCA ACAACTACAT GATGTGGAAC 
CTGGTACGCA AAACAAGCTC CTTCCTGGAC CAGCGCTTTC AGGACGCCGA CGAGAAGTTC 
ATGGAGGTTA TGTATGGGAC GAAGAAGACC TGTATTCCCC GCTGGAAGTT TTGTGTGAGT 
GACACGGAAA ACAACCTGGG CTTTGCCCTG GGACCCATGT TTGTGAAAGC AACCTTTGCA 
GAAGACAGTA AGAATATAGC CAGTGAGATC ATCATGGAGA TCAAGAAGGC GTTTGAGGAG 
AGTCTGAGCA CTCTGAAATG GATGGATGAA GAGACTCGGA GATCAGCCAA GGAGAAGGCG 
GATGCTATCT ACAACATGAT AGGCTACCCC AACTTCATCA TGGACCCCAA GGAGCTGGAC 
AAAGTCTTCA ATGACTACAC AGCAGTTCCT GATCTCTACT TTGAGAACGC CATGCGCTTT 
TTCAACTTCT CATGGAGGGT CACAGCTGAC CAGCTCAGAA AAGCTCCCAA CAGAGATCAG 
TGGAGTATGA CGCCGCCCAT GGTGAACGCT TACTACTCGC CCACCAAGAA CGAGATCGTG 
TTTCCAGCTG GAATACTGCA GGCGCCATTT TATACCCGCT CTTCACCCAA CGCCTTGAAC 
TTCGGTGGCA TTGGTGTCGT AGTGGGCCAC GAGCTGACTC ACGCTTTCGA TGATCAAGGT 
CGGGAGTACG ACAAGGATGG GAACCTTCGA CCCTGGTGGA AGAACTCGTC CGTGGAGGCG 
TTCAAGCAGC AGACGGAGTG CATGGTGCAG CAATACAGCA ACTACAGCGT GAACGGGGAG 
CCCGTGAACG GTCGACACAC CCTCGGGGAG AACATTGCCG ACAACGGGGG ACTCAAGGCG 
GCCTACCGGG CGTACCAGAA CTGGGTAAAG AAGAACGGAG CTGAGCAGAC GCTGCCCACC 
CTGGGTCTCA CCAGCAACCA GCTCTTCTTC CTAGGATTTG CACAGGTCTG GTGCTCTGTC 
CGCACACCTG AGAGCTCCCA CGAAGGCCTC ATCACGGATC CGCACAGCCC CTCTCGCTTC 
CGGGTCATCG GCTCGCTCTC CAACTCCAAG GAGTTCTCAG AACACTTTCG CTGCCCGCCT 
GGCTCTCCCA TGAACCCTCA TCACAAGTGC GAAGTCTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356108.1
CDS	103..2364
Translation	MSTYKRATLDEEDLVDSLSEGDVYPNGLQVNFRSSRSGQRCWAARTSVEKRLVVLVTLLAAGLVACLAALGIQYQTRTPPVCLTEACVSVTSSILNSMDPTVDPCQDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAVIKHLLENATASVSEAERKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEDAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLRQVSTLINNTDKCLLNNYMMWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCIPRWKFCVSDTENNLGFALGPMFVKATFAEDSKNIASEIIMEIKKAFEESLSTLKWMDEETRRSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVQQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTSNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	NM_001369179.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369179.1

ORF Insert Sequence:

ATGTCAACCT ACAAGCGGGC CACGCTGGAC GAAGAGGATC TGGTGGACTC ACTCTCCGAG 
GGCGATGTGT ACCCCAATGG CCTACAGGTG AACTTCCGCA GCTCCCGGAG CGGCCAGAGG 
TGCTGGGCAG CTCGGACCTC GGTGGAGAAG CGGCTGGTGG TGCTGGTGAC GCTTCTGGCG 
GCAGGGTTGG TGGCCTGCCT GGCAGCCCTG GGCATCCAGT ACCAGACAAG AACGCCTCCG 
GTATGTCTGA CGGAGGCCTG TGTCTCAGTG ACCAGCTCTA TCCTAAACTC CATGGACCCC 
ACAGTAGACC CGTGCCAGGA CTTCTTCAGC TACGCCTGCG GGGGCTGGAT CAAGGCCAAC 
CCCGTGCCCG ATGGCCACTC ACGCTGGGGA ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCTGTCATCA AGCATCTACT GGAAAATGCC ACGGCCAGTG TGAGCGAGGC AGAGAGAAAA 
GCCCAAGTGT ACTACCGTGC GTGCATGAAC GAAACTAGGA TTGAGGAGCT TCGGGCCAAG 
CCCCTGATGG AGCTGATTGA GAAGCTGGGA GGCTGGAATA TCACAGGACC CTGGGCCAAG 
GACAACTTCC AGGACACACT GCAGGTGGTC ACAGCTCACT ACCGCACCTC ACCCTTCTTC 
TCTGTTTATG TCAGCGCGGA CTCCAAGAAT TCCAACAGCA ATGTGATCCA GGTGGACCAG 
TCCGGCCTTG GCTTGCCCTC CAGAGACTAT TACCTGAACA AGACAGAGAA TGAAAAGGTC 
CTGACTGGAT ACCTGAACTA CATGGTCCAG CTGGGGAAGC TCCTGGGCGG TGGGGACGAG 
GATGCCATCC GGCCACAGAT GCAGCAGATC CTGGATTTTG AGACGGCGCT GGCCAACATC 
ACCATCCCCC AGGAGAAGCG CAGGGATGAA GAACTCATCT ACCACAAAGT CACGGCTGCC 
GAGCTGCAGA CCTTGGCACC TGCCATCAAC TGGTTACCCT TTCTGAACAC CATTTTTTAC 
CCAGTGGAGA TCAATGAGTC TGAGCCAATC GTGGTCTACG ACAAGGAATA CTTGAGACAA 
GTCTCCACCC TCATCAACAA CACCGACAAA TGTCTGCTCA ACAACTACAT GATGTGGAAC 
CTGGTACGCA AAACAAGCTC CTTCCTGGAC CAGCGCTTTC AGGACGCCGA CGAGAAGTTC 
ATGGAGGTTA TGTATGGGAC GAAGAAGACC TGTATTCCCC GCTGGAAGTT TTGTGTGAGT 
GACACGGAAA ACAACCTGGG CTTTGCCCTG GGACCCATGT TTGTGAAAGC AACCTTTGCA 
GAAGACAGTA AGAATATAGC CAGTGAGATC ATCATGGAGA TCAAGAAGGC GTTTGAGGAG 
AGTCTGAGCA CTCTGAAATG GATGGATGAA GAGACTCGGA GATCAGCCAA GGAGAAGGCG 
GATGCTATCT ACAACATGAT AGGCTACCCC AACTTCATCA TGGACCCCAA GGAGCTGGAC 
AAAGTCTTCA ATGACTACAC AGCAGTTCCT GATCTCTACT TTGAGAACGC CATGCGCTTT 
TTCAACTTCT CATGGAGGGT CACAGCTGAC CAGCTCAGAA AAGCTCCCAA CAGAGATCAG 
TGGAGTATGA CGCCGCCCAT GGTGAACGCT TACTACTCGC CCACCAAGAA CGAGATCGTG 
TTTCCAGCTG GAATACTGCA GGCGCCATTT TATACCCGCT CTTCACCCAA CGCCTTGAAC 
TTCGGTGGCA TTGGTGTCGT AGTGGGCCAC GAGCTGACTC ACGCTTTCGA TGATCAAGGT 
CGGGAGTACG ACAAGGATGG GAACCTTCGA CCCTGGTGGA AGAACTCGTC CGTGGAGGCG 
TTCAAGCAGC AGACGGAGTG CATGGTGCAG CAATACAGCA ACTACAGCGT GAACGGGGAG 
CCCGTGAACG GTCGACACAC CCTCGGGGAG AACATTGCCG ACAACGGGGG ACTCAAGGCG 
GCCTACCGGG CGTACCAGAA CTGGGTAAAG AAGAACGGAG CTGAGCAGAC GCTGCCCACC 
CTGGGTCTCA CCAGCAACCA GCTCTTCTTC CTAGGATTTG CACAGGTCTG GTGCTCTGTC 
CGCACACCTG AGAGCTCCCA CGAAGGCCTC ATCACGGATC CGCACAGCCC CTCTCGCTTC 
CGGGTCATCG GCTCGCTCTC CAACTCCAAG GAGTTCTCAG AACACTTTCG CTGCCCGCCT 
GGCTCTCCCA TGAACCCTCA TCACAAGTGC GAAGTCTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ece1 ( NM_199307.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_955011.1 Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant 3, mRNA.

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CloneID	OMu09434
Clone ID Related Accession (Same CDS sequence)	XM_006538767.3 , XM_006538767.2 , NM_001369179.1 , NM_199307.2
Accession Version	NM_199307.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	endothelin-converting enzyme 1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK134088.1, BG083532.2, BC060648.1 and BQ749244.1. On Aug 1, 2009 this sequence version replaced NM_199307.1. Transcript Variant: This variant (3), as well as variant 4, encodes isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060648.1, AK134088.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAACCT ACAAGCGGGC CACGCTGGAC GAAGAGGATC TGGTGGACTC ACTCTCCGAG 
GGCGATGTGT ACCCCAATGG CCTACAGGTG AACTTCCGCA GCTCCCGGAG CGGCCAGAGG 
TGCTGGGCAG CTCGGACCTC GGTGGAGAAG CGGCTGGTGG TGCTGGTGAC GCTTCTGGCG 
GCAGGGTTGG TGGCCTGCCT GGCAGCCCTG GGCATCCAGT ACCAGACAAG AACGCCTCCG 
GTATGTCTGA CGGAGGCCTG TGTCTCAGTG ACCAGCTCTA TCCTAAACTC CATGGACCCC 
ACAGTAGACC CGTGCCAGGA CTTCTTCAGC TACGCCTGCG GGGGCTGGAT CAAGGCCAAC 
CCCGTGCCCG ATGGCCACTC ACGCTGGGGA ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCTGTCATCA AGCATCTACT GGAAAATGCC ACGGCCAGTG TGAGCGAGGC AGAGAGAAAA 
GCCCAAGTGT ACTACCGTGC GTGCATGAAC GAAACTAGGA TTGAGGAGCT TCGGGCCAAG 
CCCCTGATGG AGCTGATTGA GAAGCTGGGA GGCTGGAATA TCACAGGACC CTGGGCCAAG 
GACAACTTCC AGGACACACT GCAGGTGGTC ACAGCTCACT ACCGCACCTC ACCCTTCTTC 
TCTGTTTATG TCAGCGCGGA CTCCAAGAAT TCCAACAGCA ATGTGATCCA GGTGGACCAG 
TCCGGCCTTG GCTTGCCCTC CAGAGACTAT TACCTGAACA AGACAGAGAA TGAAAAGGTC 
CTGACTGGAT ACCTGAACTA CATGGTCCAG CTGGGGAAGC TCCTGGGCGG TGGGGACGAG 
GATGCCATCC GGCCACAGAT GCAGCAGATC CTGGATTTTG AGACGGCGCT GGCCAACATC 
ACCATCCCCC AGGAGAAGCG CAGGGATGAA GAACTCATCT ACCACAAAGT CACGGCTGCC 
GAGCTGCAGA CCTTGGCACC TGCCATCAAC TGGTTACCCT TTCTGAACAC CATTTTTTAC 
CCAGTGGAGA TCAATGAGTC TGAGCCAATC GTGGTCTACG ACAAGGAATA CTTGAGACAA 
GTCTCCACCC TCATCAACAA CACCGACAAA TGTCTGCTCA ACAACTACAT GATGTGGAAC 
CTGGTACGCA AAACAAGCTC CTTCCTGGAC CAGCGCTTTC AGGACGCCGA CGAGAAGTTC 
ATGGAGGTTA TGTATGGGAC GAAGAAGACC TGTATTCCCC GCTGGAAGTT TTGTGTGAGT 
GACACGGAAA ACAACCTGGG CTTTGCCCTG GGACCCATGT TTGTGAAAGC AACCTTTGCA 
GAAGACAGTA AGAATATAGC CAGTGAGATC ATCATGGAGA TCAAGAAGGC GTTTGAGGAG 
AGTCTGAGCA CTCTGAAATG GATGGATGAA GAGACTCGGA GATCAGCCAA GGAGAAGGCG 
GATGCTATCT ACAACATGAT AGGCTACCCC AACTTCATCA TGGACCCCAA GGAGCTGGAC 
AAAGTCTTCA ATGACTACAC AGCAGTTCCT GATCTCTACT TTGAGAACGC CATGCGCTTT 
TTCAACTTCT CATGGAGGGT CACAGCTGAC CAGCTCAGAA AAGCTCCCAA CAGAGATCAG 
TGGAGTATGA CGCCGCCCAT GGTGAACGCT TACTACTCGC CCACCAAGAA CGAGATCGTG 
TTTCCAGCTG GAATACTGCA GGCGCCATTT TATACCCGCT CTTCACCCAA CGCCTTGAAC 
TTCGGTGGCA TTGGTGTCGT AGTGGGCCAC GAGCTGACTC ACGCTTTCGA TGATCAAGGT 
CGGGAGTACG ACAAGGATGG GAACCTTCGA CCCTGGTGGA AGAACTCGTC CGTGGAGGCG 
TTCAAGCAGC AGACGGAGTG CATGGTGCAG CAATACAGCA ACTACAGCGT GAACGGGGAG 
CCCGTGAACG GTCGACACAC CCTCGGGGAG AACATTGCCG ACAACGGGGG ACTCAAGGCG 
GCCTACCGGG CGTACCAGAA CTGGGTAAAG AAGAACGGAG CTGAGCAGAC GCTGCCCACC 
CTGGGTCTCA CCAGCAACCA GCTCTTCTTC CTAGGATTTG CACAGGTCTG GTGCTCTGTC 
CGCACACCTG AGAGCTCCCA CGAAGGCCTC ATCACGGATC CGCACAGCCC CTCTCGCTTC 
CGGGTCATCG GCTCGCTCTC CAACTCCAAG GAGTTCTCAG AACACTTTCG CTGCCCGCCT 
GGCTCTCCCA TGAACCCTCA TCACAAGTGC GAAGTCTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955011.1
CDS	232..2493
Translation	MSTYKRATLDEEDLVDSLSEGDVYPNGLQVNFRSSRSGQRCWAARTSVEKRLVVLVTLLAAGLVACLAALGIQYQTRTPPVCLTEACVSVTSSILNSMDPTVDPCQDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAVIKHLLENATASVSEAERKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEDAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLRQVSTLINNTDKCLLNNYMMWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCIPRWKFCVSDTENNLGFALGPMFVKATFAEDSKNIASEIIMEIKKAFEESLSTLKWMDEETRRSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVQQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTSNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	NM_199307.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199307.2

ORF Insert Sequence:

ATGTCAACCT ACAAGCGGGC CACGCTGGAC GAAGAGGATC TGGTGGACTC ACTCTCCGAG 
GGCGATGTGT ACCCCAATGG CCTACAGGTG AACTTCCGCA GCTCCCGGAG CGGCCAGAGG 
TGCTGGGCAG CTCGGACCTC GGTGGAGAAG CGGCTGGTGG TGCTGGTGAC GCTTCTGGCG 
GCAGGGTTGG TGGCCTGCCT GGCAGCCCTG GGCATCCAGT ACCAGACAAG AACGCCTCCG 
GTATGTCTGA CGGAGGCCTG TGTCTCAGTG ACCAGCTCTA TCCTAAACTC CATGGACCCC 
ACAGTAGACC CGTGCCAGGA CTTCTTCAGC TACGCCTGCG GGGGCTGGAT CAAGGCCAAC 
CCCGTGCCCG ATGGCCACTC ACGCTGGGGA ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCTGTCATCA AGCATCTACT GGAAAATGCC ACGGCCAGTG TGAGCGAGGC AGAGAGAAAA 
GCCCAAGTGT ACTACCGTGC GTGCATGAAC GAAACTAGGA TTGAGGAGCT TCGGGCCAAG 
CCCCTGATGG AGCTGATTGA GAAGCTGGGA GGCTGGAATA TCACAGGACC CTGGGCCAAG 
GACAACTTCC AGGACACACT GCAGGTGGTC ACAGCTCACT ACCGCACCTC ACCCTTCTTC 
TCTGTTTATG TCAGCGCGGA CTCCAAGAAT TCCAACAGCA ATGTGATCCA GGTGGACCAG 
TCCGGCCTTG GCTTGCCCTC CAGAGACTAT TACCTGAACA AGACAGAGAA TGAAAAGGTC 
CTGACTGGAT ACCTGAACTA CATGGTCCAG CTGGGGAAGC TCCTGGGCGG TGGGGACGAG 
GATGCCATCC GGCCACAGAT GCAGCAGATC CTGGATTTTG AGACGGCGCT GGCCAACATC 
ACCATCCCCC AGGAGAAGCG CAGGGATGAA GAACTCATCT ACCACAAAGT CACGGCTGCC 
GAGCTGCAGA CCTTGGCACC TGCCATCAAC TGGTTACCCT TTCTGAACAC CATTTTTTAC 
CCAGTGGAGA TCAATGAGTC TGAGCCAATC GTGGTCTACG ACAAGGAATA CTTGAGACAA 
GTCTCCACCC TCATCAACAA CACCGACAAA TGTCTGCTCA ACAACTACAT GATGTGGAAC 
CTGGTACGCA AAACAAGCTC CTTCCTGGAC CAGCGCTTTC AGGACGCCGA CGAGAAGTTC 
ATGGAGGTTA TGTATGGGAC GAAGAAGACC TGTATTCCCC GCTGGAAGTT TTGTGTGAGT 
GACACGGAAA ACAACCTGGG CTTTGCCCTG GGACCCATGT TTGTGAAAGC AACCTTTGCA 
GAAGACAGTA AGAATATAGC CAGTGAGATC ATCATGGAGA TCAAGAAGGC GTTTGAGGAG 
AGTCTGAGCA CTCTGAAATG GATGGATGAA GAGACTCGGA GATCAGCCAA GGAGAAGGCG 
GATGCTATCT ACAACATGAT AGGCTACCCC AACTTCATCA TGGACCCCAA GGAGCTGGAC 
AAAGTCTTCA ATGACTACAC AGCAGTTCCT GATCTCTACT TTGAGAACGC CATGCGCTTT 
TTCAACTTCT CATGGAGGGT CACAGCTGAC CAGCTCAGAA AAGCTCCCAA CAGAGATCAG 
TGGAGTATGA CGCCGCCCAT GGTGAACGCT TACTACTCGC CCACCAAGAA CGAGATCGTG 
TTTCCAGCTG GAATACTGCA GGCGCCATTT TATACCCGCT CTTCACCCAA CGCCTTGAAC 
TTCGGTGGCA TTGGTGTCGT AGTGGGCCAC GAGCTGACTC ACGCTTTCGA TGATCAAGGT 
CGGGAGTACG ACAAGGATGG GAACCTTCGA CCCTGGTGGA AGAACTCGTC CGTGGAGGCG 
TTCAAGCAGC AGACGGAGTG CATGGTGCAG CAATACAGCA ACTACAGCGT GAACGGGGAG 
CCCGTGAACG GTCGACACAC CCTCGGGGAG AACATTGCCG ACAACGGGGG ACTCAAGGCG 
GCCTACCGGG CGTACCAGAA CTGGGTAAAG AAGAACGGAG CTGAGCAGAC GCTGCCCACC 
CTGGGTCTCA CCAGCAACCA GCTCTTCTTC CTAGGATTTG CACAGGTCTG GTGCTCTGTC 
CGCACACCTG AGAGCTCCCA CGAAGGCCTC ATCACGGATC CGCACAGCCC CTCTCGCTTC 
CGGGTCATCG GCTCGCTCTC CAACTCCAAG GAGTTCTCAG AACACTTTCG CTGCCCGCCT 
GGCTCTCCCA TGAACCCTCA TCACAAGTGC GAAGTCTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ece1 ( NM_001369178.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001356107.1 Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant 2, mRNA.

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CloneID	OMu84948
Clone ID Related Accession (Same CDS sequence)	NM_001369178.1
Accession Version	NM_001369178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	endothelin-converting enzyme 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK163790.1 and AL807764.9. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7974084.23047.1, AK163790.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCTCCCC AGAGTCTGGG CCTGCAGCGG GGTTCCTTCT TCCTGGGGAA GAGGGGCCCT 
GGTCTCATGG TCTCGCTGCC CCTCCTGGCT TCTTCCCTGC AGGTGAACTT CCGCAGCTCC 
CGGAGCGGCC AGAGGTGCTG GGCAGCTCGG ACCTCGGTGG AGAAGCGGCT GGTGGTGCTG 
GTGACGCTTC TGGCGGCAGG GTTGGTGGCC TGCCTGGCAG CCCTGGGCAT CCAGTACCAG 
ACAAGAACGC CTCCGGTATG TCTGACGGAG GCCTGTGTCT CAGTGACCAG CTCTATCCTA 
AACTCCATGG ACCCCACAGT AGACCCGTGC CAGGACTTCT TCAGCTACGC CTGCGGGGGC 
TGGATCAAGG CCAACCCCGT GCCCGATGGC CACTCACGCT GGGGAACCTT CAGCAACCTC 
TGGGAACACA ACCAAGCTGT CATCAAGCAT CTACTGGAAA ATGCCACGGC CAGTGTGAGC 
GAGGCAGAGA GAAAAGCCCA AGTGTACTAC CGTGCGTGCA TGAACGAAAC TAGGATTGAG 
GAGCTTCGGG CCAAGCCCCT GATGGAGCTG ATTGAGAAGC TGGGAGGCTG GAATATCACA 
GGACCCTGGG CCAAGGACAA CTTCCAGGAC ACACTGCAGG TGGTCACAGC TCACTACCGC 
ACCTCACCCT TCTTCTCTGT TTATGTCAGC GCGGACTCCA AGAATTCCAA CAGCAATGTG 
ATCCAGGTGG ACCAGTCCGG CCTTGGCTTG CCCTCCAGAG ACTATTACCT GAACAAGACA 
GAGAATGAAA AGGTCCTGAC TGGATACCTG AACTACATGG TCCAGCTGGG GAAGCTCCTG 
GGCGGTGGGG ACGAGGATGC CATCCGGCCA CAGATGCAGC AGATCCTGGA TTTTGAGACG 
GCGCTGGCCA ACATCACCAT CCCCCAGGAG AAGCGCAGGG ATGAAGAACT CATCTACCAC 
AAAGTCACGG CTGCCGAGCT GCAGACCTTG GCACCTGCCA TCAACTGGTT ACCCTTTCTG 
AACACCATTT TTTACCCAGT GGAGATCAAT GAGTCTGAGC CAATCGTGGT CTACGACAAG 
GAATACTTGA GACAAGTCTC CACCCTCATC AACAACACCG ACAAATGTCT GCTCAACAAC 
TACATGATGT GGAACCTGGT ACGCAAAACA AGCTCCTTCC TGGACCAGCG CTTTCAGGAC 
GCCGACGAGA AGTTCATGGA GGTTATGTAT GGGACGAAGA AGACCTGTAT TCCCCGCTGG 
AAGTTTTGTG TGAGTGACAC GGAAAACAAC CTGGGCTTTG CCCTGGGACC CATGTTTGTG 
AAAGCAACCT TTGCAGAAGA CAGTAAGAAT ATAGCCAGTG AGATCATCAT GGAGATCAAG 
AAGGCGTTTG AGGAGAGTCT GAGCACTCTG AAATGGATGG ATGAAGAGAC TCGGAGATCA 
GCCAAGGAGA AGGCGGATGC TATCTACAAC ATGATAGGCT ACCCCAACTT CATCATGGAC 
CCCAAGGAGC TGGACAAAGT CTTCAATGAC TACACAGCAG TTCCTGATCT CTACTTTGAG 
AACGCCATGC GCTTTTTCAA CTTCTCATGG AGGGTCACAG CTGACCAGCT CAGAAAAGCT 
CCCAACAGAG ATCAGTGGAG TATGACGCCG CCCATGGTGA ACGCTTACTA CTCGCCCACC 
AAGAACGAGA TCGTGTTTCC AGCTGGAATA CTGCAGGCGC CATTTTATAC CCGCTCTTCA 
CCCAACGCCT TGAACTTCGG TGGCATTGGT GTCGTAGTGG GCCACGAGCT GACTCACGCT 
TTCGATGATC AAGGTCGGGA GTACGACAAG GATGGGAACC TTCGACCCTG GTGGAAGAAC 
TCGTCCGTGG AGGCGTTCAA GCAGCAGACG GAGTGCATGG TGCAGCAATA CAGCAACTAC 
AGCGTGAACG GGGAGCCCGT GAACGGTCGA CACACCCTCG GGGAGAACAT TGCCGACAAC 
GGGGGACTCA AGGCGGCCTA CCGGGCGTAC CAGAACTGGG TAAAGAAGAA CGGAGCTGAG 
CAGACGCTGC CCACCCTGGG TCTCACCAGC AACCAGCTCT TCTTCCTAGG ATTTGCACAG 
GTCTGGTGCT CTGTCCGCAC ACCTGAGAGC TCCCACGAAG GCCTCATCAC GGATCCGCAC 
AGCCCCTCTC GCTTCCGGGT CATCGGCTCG CTCTCCAACT CCAAGGAGTT CTCAGAACAC 
TTTCGCTGCC CGCCTGGCTC TCCCATGAAC CCTCATCACA AGTGCGAAGT CTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356107.1
CDS	127..2403
Translation	MPPQSLGLQRGSFFLGKRGPGLMVSLPLLASSLQVNFRSSRSGQRCWAARTSVEKRLVVLVTLLAAGLVACLAALGIQYQTRTPPVCLTEACVSVTSSILNSMDPTVDPCQDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAVIKHLLENATASVSEAERKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEDAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLRQVSTLINNTDKCLLNNYMMWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCIPRWKFCVSDTENNLGFALGPMFVKATFAEDSKNIASEIIMEIKKAFEESLSTLKWMDEETRRSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVQQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTSNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	NM_001369178.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369178.1

ORF Insert Sequence:

ATGCCTCCCC AGAGTCTGGG CCTGCAGCGG GGTTCCTTCT TCCTGGGGAA GAGGGGCCCT 
GGTCTCATGG TCTCGCTGCC CCTCCTGGCT TCTTCCCTGC AGGTGAACTT CCGCAGCTCC 
CGGAGCGGCC AGAGGTGCTG GGCAGCTCGG ACCTCGGTGG AGAAGCGGCT GGTGGTGCTG 
GTGACGCTTC TGGCGGCAGG GTTGGTGGCC TGCCTGGCAG CCCTGGGCAT CCAGTACCAG 
ACAAGAACGC CTCCGGTATG TCTGACGGAG GCCTGTGTCT CAGTGACCAG CTCTATCCTA 
AACTCCATGG ACCCCACAGT AGACCCGTGC CAGGACTTCT TCAGCTACGC CTGCGGGGGC 
TGGATCAAGG CCAACCCCGT GCCCGATGGC CACTCACGCT GGGGAACCTT CAGCAACCTC 
TGGGAACACA ACCAAGCTGT CATCAAGCAT CTACTGGAAA ATGCCACGGC CAGTGTGAGC 
GAGGCAGAGA GAAAAGCCCA AGTGTACTAC CGTGCGTGCA TGAACGAAAC TAGGATTGAG 
GAGCTTCGGG CCAAGCCCCT GATGGAGCTG ATTGAGAAGC TGGGAGGCTG GAATATCACA 
GGACCCTGGG CCAAGGACAA CTTCCAGGAC ACACTGCAGG TGGTCACAGC TCACTACCGC 
ACCTCACCCT TCTTCTCTGT TTATGTCAGC GCGGACTCCA AGAATTCCAA CAGCAATGTG 
ATCCAGGTGG ACCAGTCCGG CCTTGGCTTG CCCTCCAGAG ACTATTACCT GAACAAGACA 
GAGAATGAAA AGGTCCTGAC TGGATACCTG AACTACATGG TCCAGCTGGG GAAGCTCCTG 
GGCGGTGGGG ACGAGGATGC CATCCGGCCA CAGATGCAGC AGATCCTGGA TTTTGAGACG 
GCGCTGGCCA ACATCACCAT CCCCCAGGAG AAGCGCAGGG ATGAAGAACT CATCTACCAC 
AAAGTCACGG CTGCCGAGCT GCAGACCTTG GCACCTGCCA TCAACTGGTT ACCCTTTCTG 
AACACCATTT TTTACCCAGT GGAGATCAAT GAGTCTGAGC CAATCGTGGT CTACGACAAG 
GAATACTTGA GACAAGTCTC CACCCTCATC AACAACACCG ACAAATGTCT GCTCAACAAC 
TACATGATGT GGAACCTGGT ACGCAAAACA AGCTCCTTCC TGGACCAGCG CTTTCAGGAC 
GCCGACGAGA AGTTCATGGA GGTTATGTAT GGGACGAAGA AGACCTGTAT TCCCCGCTGG 
AAGTTTTGTG TGAGTGACAC GGAAAACAAC CTGGGCTTTG CCCTGGGACC CATGTTTGTG 
AAAGCAACCT TTGCAGAAGA CAGTAAGAAT ATAGCCAGTG AGATCATCAT GGAGATCAAG 
AAGGCGTTTG AGGAGAGTCT GAGCACTCTG AAATGGATGG ATGAAGAGAC TCGGAGATCA 
GCCAAGGAGA AGGCGGATGC TATCTACAAC ATGATAGGCT ACCCCAACTT CATCATGGAC 
CCCAAGGAGC TGGACAAAGT CTTCAATGAC TACACAGCAG TTCCTGATCT CTACTTTGAG 
AACGCCATGC GCTTTTTCAA CTTCTCATGG AGGGTCACAG CTGACCAGCT CAGAAAAGCT 
CCCAACAGAG ATCAGTGGAG TATGACGCCG CCCATGGTGA ACGCTTACTA CTCGCCCACC 
AAGAACGAGA TCGTGTTTCC AGCTGGAATA CTGCAGGCGC CATTTTATAC CCGCTCTTCA 
CCCAACGCCT TGAACTTCGG TGGCATTGGT GTCGTAGTGG GCCACGAGCT GACTCACGCT 
TTCGATGATC AAGGTCGGGA GTACGACAAG GATGGGAACC TTCGACCCTG GTGGAAGAAC 
TCGTCCGTGG AGGCGTTCAA GCAGCAGACG GAGTGCATGG TGCAGCAATA CAGCAACTAC 
AGCGTGAACG GGGAGCCCGT GAACGGTCGA CACACCCTCG GGGAGAACAT TGCCGACAAC 
GGGGGACTCA AGGCGGCCTA CCGGGCGTAC CAGAACTGGG TAAAGAAGAA CGGAGCTGAG 
CAGACGCTGC CCACCCTGGG TCTCACCAGC AACCAGCTCT TCTTCCTAGG ATTTGCACAG 
GTCTGGTGCT CTGTCCGCAC ACCTGAGAGC TCCCACGAAG GCCTCATCAC GGATCCGCAC 
AGCCCCTCTC GCTTCCGGGT CATCGGCTCG CTCTCCAACT CCAAGGAGTT CTCAGAACAC 
TTTCGCTGCC CGCCTGGCTC TCCCATGAAC CCTCATCACA AGTGCGAAGT CTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ece1 ( XM_006538767.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538830.1 Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant X2, mRNA.

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CloneID	OMu09434
Clone ID Related Accession (Same CDS sequence)	XM_006538767.3 , XM_006538767.2 , NM_001369179.1 , NM_199307.2
Accession Version	XM_006538767.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	endothelin-converting enzyme 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538767.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACCT ACAAGCGGGC CACGCTGGAC GAAGAGGATC TGGTGGACTC ACTCTCCGAG 
GGCGATGTGT ACCCCAATGG CCTACAGGTG AACTTCCGCA GCTCCCGGAG CGGCCAGAGG 
TGCTGGGCAG CTCGGACCTC GGTGGAGAAG CGGCTGGTGG TGCTGGTGAC GCTTCTGGCG 
GCAGGGTTGG TGGCCTGCCT GGCAGCCCTG GGCATCCAGT ACCAGACAAG AACGCCTCCG 
GTATGTCTGA CGGAGGCCTG TGTCTCAGTG ACCAGCTCTA TCCTAAACTC CATGGACCCC 
ACAGTAGACC CGTGCCAGGA CTTCTTCAGC TACGCCTGCG GGGGCTGGAT CAAGGCCAAC 
CCCGTGCCCG ATGGCCACTC ACGCTGGGGA ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCTGTCATCA AGCATCTACT GGAAAATGCC ACGGCCAGTG TGAGCGAGGC AGAGAGAAAA 
GCCCAAGTGT ACTACCGTGC GTGCATGAAC GAAACTAGGA TTGAGGAGCT TCGGGCCAAG 
CCCCTGATGG AGCTGATTGA GAAGCTGGGA GGCTGGAATA TCACAGGACC CTGGGCCAAG 
GACAACTTCC AGGACACACT GCAGGTGGTC ACAGCTCACT ACCGCACCTC ACCCTTCTTC 
TCTGTTTATG TCAGCGCGGA CTCCAAGAAT TCCAACAGCA ATGTGATCCA GGTGGACCAG 
TCCGGCCTTG GCTTGCCCTC CAGAGACTAT TACCTGAACA AGACAGAGAA TGAAAAGGTC 
CTGACTGGAT ACCTGAACTA CATGGTCCAG CTGGGGAAGC TCCTGGGCGG TGGGGACGAG 
GATGCCATCC GGCCACAGAT GCAGCAGATC CTGGATTTTG AGACGGCGCT GGCCAACATC 
ACCATCCCCC AGGAGAAGCG CAGGGATGAA GAACTCATCT ACCACAAAGT CACGGCTGCC 
GAGCTGCAGA CCTTGGCACC TGCCATCAAC TGGTTACCCT TTCTGAACAC CATTTTTTAC 
CCAGTGGAGA TCAATGAGTC TGAGCCAATC GTGGTCTACG ACAAGGAATA CTTGAGACAA 
GTCTCCACCC TCATCAACAA CACCGACAAA TGTCTGCTCA ACAACTACAT GATGTGGAAC 
CTGGTACGCA AAACAAGCTC CTTCCTGGAC CAGCGCTTTC AGGACGCCGA CGAGAAGTTC 
ATGGAGGTTA TGTATGGGAC GAAGAAGACC TGTATTCCCC GCTGGAAGTT TTGTGTGAGT 
GACACGGAAA ACAACCTGGG CTTTGCCCTG GGACCCATGT TTGTGAAAGC AACCTTTGCA 
GAAGACAGTA AGAATATAGC CAGTGAGATC ATCATGGAGA TCAAGAAGGC GTTTGAGGAG 
AGTCTGAGCA CTCTGAAATG GATGGATGAA GAGACTCGGA GATCAGCCAA GGAGAAGGCG 
GATGCTATCT ACAACATGAT AGGCTACCCC AACTTCATCA TGGACCCCAA GGAGCTGGAC 
AAAGTCTTCA ATGACTACAC AGCAGTTCCT GATCTCTACT TTGAGAACGC CATGCGCTTT 
TTCAACTTCT CATGGAGGGT CACAGCTGAC CAGCTCAGAA AAGCTCCCAA CAGAGATCAG 
TGGAGTATGA CGCCGCCCAT GGTGAACGCT TACTACTCGC CCACCAAGAA CGAGATCGTG 
TTTCCAGCTG GAATACTGCA GGCGCCATTT TATACCCGCT CTTCACCCAA CGCCTTGAAC 
TTCGGTGGCA TTGGTGTCGT AGTGGGCCAC GAGCTGACTC ACGCTTTCGA TGATCAAGGT 
CGGGAGTACG ACAAGGATGG GAACCTTCGA CCCTGGTGGA AGAACTCGTC CGTGGAGGCG 
TTCAAGCAGC AGACGGAGTG CATGGTGCAG CAATACAGCA ACTACAGCGT GAACGGGGAG 
CCCGTGAACG GTCGACACAC CCTCGGGGAG AACATTGCCG ACAACGGGGG ACTCAAGGCG 
GCCTACCGGG CGTACCAGAA CTGGGTAAAG AAGAACGGAG CTGAGCAGAC GCTGCCCACC 
CTGGGTCTCA CCAGCAACCA GCTCTTCTTC CTAGGATTTG CACAGGTCTG GTGCTCTGTC 
CGCACACCTG AGAGCTCCCA CGAAGGCCTC ATCACGGATC CGCACAGCCC CTCTCGCTTC 
CGGGTCATCG GCTCGCTCTC CAACTCCAAG GAGTTCTCAG AACACTTTCG CTGCCCGCCT 
GGCTCTCCCA TGAACCCTCA TCACAAGTGC GAAGTCTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538830.1
CDS	171..2432
Translation	MSTYKRATLDEEDLVDSLSEGDVYPNGLQVNFRSSRSGQRCWAARTSVEKRLVVLVTLLAAGLVACLAALGIQYQTRTPPVCLTEACVSVTSSILNSMDPTVDPCQDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAVIKHLLENATASVSEAERKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEDAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLRQVSTLINNTDKCLLNNYMMWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCIPRWKFCVSDTENNLGFALGPMFVKATFAEDSKNIASEIIMEIKKAFEESLSTLKWMDEETRRSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVQQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTSNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	XM_006538767.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538767.3

ORF Insert Sequence:

ATGTCAACCT ACAAGCGGGC CACGCTGGAC GAAGAGGATC TGGTGGACTC ACTCTCCGAG 
GGCGATGTGT ACCCCAATGG CCTACAGGTG AACTTCCGCA GCTCCCGGAG CGGCCAGAGG 
TGCTGGGCAG CTCGGACCTC GGTGGAGAAG CGGCTGGTGG TGCTGGTGAC GCTTCTGGCG 
GCAGGGTTGG TGGCCTGCCT GGCAGCCCTG GGCATCCAGT ACCAGACAAG AACGCCTCCG 
GTATGTCTGA CGGAGGCCTG TGTCTCAGTG ACCAGCTCTA TCCTAAACTC CATGGACCCC 
ACAGTAGACC CGTGCCAGGA CTTCTTCAGC TACGCCTGCG GGGGCTGGAT CAAGGCCAAC 
CCCGTGCCCG ATGGCCACTC ACGCTGGGGA ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCTGTCATCA AGCATCTACT GGAAAATGCC ACGGCCAGTG TGAGCGAGGC AGAGAGAAAA 
GCCCAAGTGT ACTACCGTGC GTGCATGAAC GAAACTAGGA TTGAGGAGCT TCGGGCCAAG 
CCCCTGATGG AGCTGATTGA GAAGCTGGGA GGCTGGAATA TCACAGGACC CTGGGCCAAG 
GACAACTTCC AGGACACACT GCAGGTGGTC ACAGCTCACT ACCGCACCTC ACCCTTCTTC 
TCTGTTTATG TCAGCGCGGA CTCCAAGAAT TCCAACAGCA ATGTGATCCA GGTGGACCAG 
TCCGGCCTTG GCTTGCCCTC CAGAGACTAT TACCTGAACA AGACAGAGAA TGAAAAGGTC 
CTGACTGGAT ACCTGAACTA CATGGTCCAG CTGGGGAAGC TCCTGGGCGG TGGGGACGAG 
GATGCCATCC GGCCACAGAT GCAGCAGATC CTGGATTTTG AGACGGCGCT GGCCAACATC 
ACCATCCCCC AGGAGAAGCG CAGGGATGAA GAACTCATCT ACCACAAAGT CACGGCTGCC 
GAGCTGCAGA CCTTGGCACC TGCCATCAAC TGGTTACCCT TTCTGAACAC CATTTTTTAC 
CCAGTGGAGA TCAATGAGTC TGAGCCAATC GTGGTCTACG ACAAGGAATA CTTGAGACAA 
GTCTCCACCC TCATCAACAA CACCGACAAA TGTCTGCTCA ACAACTACAT GATGTGGAAC 
CTGGTACGCA AAACAAGCTC CTTCCTGGAC CAGCGCTTTC AGGACGCCGA CGAGAAGTTC 
ATGGAGGTTA TGTATGGGAC GAAGAAGACC TGTATTCCCC GCTGGAAGTT TTGTGTGAGT 
GACACGGAAA ACAACCTGGG CTTTGCCCTG GGACCCATGT TTGTGAAAGC AACCTTTGCA 
GAAGACAGTA AGAATATAGC CAGTGAGATC ATCATGGAGA TCAAGAAGGC GTTTGAGGAG 
AGTCTGAGCA CTCTGAAATG GATGGATGAA GAGACTCGGA GATCAGCCAA GGAGAAGGCG 
GATGCTATCT ACAACATGAT AGGCTACCCC AACTTCATCA TGGACCCCAA GGAGCTGGAC 
AAAGTCTTCA ATGACTACAC AGCAGTTCCT GATCTCTACT TTGAGAACGC CATGCGCTTT 
TTCAACTTCT CATGGAGGGT CACAGCTGAC CAGCTCAGAA AAGCTCCCAA CAGAGATCAG 
TGGAGTATGA CGCCGCCCAT GGTGAACGCT TACTACTCGC CCACCAAGAA CGAGATCGTG 
TTTCCAGCTG GAATACTGCA GGCGCCATTT TATACCCGCT CTTCACCCAA CGCCTTGAAC 
TTCGGTGGCA TTGGTGTCGT AGTGGGCCAC GAGCTGACTC ACGCTTTCGA TGATCAAGGT 
CGGGAGTACG ACAAGGATGG GAACCTTCGA CCCTGGTGGA AGAACTCGTC CGTGGAGGCG 
TTCAAGCAGC AGACGGAGTG CATGGTGCAG CAATACAGCA ACTACAGCGT GAACGGGGAG 
CCCGTGAACG GTCGACACAC CCTCGGGGAG AACATTGCCG ACAACGGGGG ACTCAAGGCG 
GCCTACCGGG CGTACCAGAA CTGGGTAAAG AAGAACGGAG CTGAGCAGAC GCTGCCCACC 
CTGGGTCTCA CCAGCAACCA GCTCTTCTTC CTAGGATTTG CACAGGTCTG GTGCTCTGTC 
CGCACACCTG AGAGCTCCCA CGAAGGCCTC ATCACGGATC CGCACAGCCC CTCTCGCTTC 
CGGGTCATCG GCTCGCTCTC CAACTCCAAG GAGTTCTCAG AACACTTTCG CTGCCCGCCT 
GGCTCTCCCA TGAACCCTCA TCACAAGTGC GAAGTCTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ece1 ( XM_006538767.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538830.1 Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu09434
Clone ID Related Accession (Same CDS sequence)	XM_006538767.3 , XM_006538767.2 , NM_001369179.1 , NM_199307.2
Accession Version	XM_006538767.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	endothelin-converting enzyme 1 isoform X1
Comment

ATGTCAACCT ACAAGCGGGC CACGCTGGAC GAAGAGGATC TGGTGGACTC ACTCTCCGAG 
GGCGATGTGT ACCCCAATGG CCTACAGGTG AACTTCCGCA GCTCCCGGAG CGGCCAGAGG 
TGCTGGGCAG CTCGGACCTC GGTGGAGAAG CGGCTGGTGG TGCTGGTGAC GCTTCTGGCG 
GCAGGGTTGG TGGCCTGCCT GGCAGCCCTG GGCATCCAGT ACCAGACAAG AACGCCTCCG 
GTATGTCTGA CGGAGGCCTG TGTCTCAGTG ACCAGCTCTA TCCTAAACTC CATGGACCCC 
ACAGTAGACC CGTGCCAGGA CTTCTTCAGC TACGCCTGCG GGGGCTGGAT CAAGGCCAAC 
CCCGTGCCCG ATGGCCACTC ACGCTGGGGA ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCTGTCATCA AGCATCTACT GGAAAATGCC ACGGCCAGTG TGAGCGAGGC AGAGAGAAAA 
GCCCAAGTGT ACTACCGTGC GTGCATGAAC GAAACTAGGA TTGAGGAGCT TCGGGCCAAG 
CCCCTGATGG AGCTGATTGA GAAGCTGGGA GGCTGGAATA TCACAGGACC CTGGGCCAAG 
GACAACTTCC AGGACACACT GCAGGTGGTC ACAGCTCACT ACCGCACCTC ACCCTTCTTC 
TCTGTTTATG TCAGCGCGGA CTCCAAGAAT TCCAACAGCA ATGTGATCCA GGTGGACCAG 
TCCGGCCTTG GCTTGCCCTC CAGAGACTAT TACCTGAACA AGACAGAGAA TGAAAAGGTC 
CTGACTGGAT ACCTGAACTA CATGGTCCAG CTGGGGAAGC TCCTGGGCGG TGGGGACGAG 
GATGCCATCC GGCCACAGAT GCAGCAGATC CTGGATTTTG AGACGGCGCT GGCCAACATC 
ACCATCCCCC AGGAGAAGCG CAGGGATGAA GAACTCATCT ACCACAAAGT CACGGCTGCC 
GAGCTGCAGA CCTTGGCACC TGCCATCAAC TGGTTACCCT TTCTGAACAC CATTTTTTAC 
CCAGTGGAGA TCAATGAGTC TGAGCCAATC GTGGTCTACG ACAAGGAATA CTTGAGACAA 
GTCTCCACCC TCATCAACAA CACCGACAAA TGTCTGCTCA ACAACTACAT GATGTGGAAC 
CTGGTACGCA AAACAAGCTC CTTCCTGGAC CAGCGCTTTC AGGACGCCGA CGAGAAGTTC 
ATGGAGGTTA TGTATGGGAC GAAGAAGACC TGTATTCCCC GCTGGAAGTT TTGTGTGAGT 
GACACGGAAA ACAACCTGGG CTTTGCCCTG GGACCCATGT TTGTGAAAGC AACCTTTGCA 
GAAGACAGTA AGAATATAGC CAGTGAGATC ATCATGGAGA TCAAGAAGGC GTTTGAGGAG 
AGTCTGAGCA CTCTGAAATG GATGGATGAA GAGACTCGGA GATCAGCCAA GGAGAAGGCG 
GATGCTATCT ACAACATGAT AGGCTACCCC AACTTCATCA TGGACCCCAA GGAGCTGGAC 
AAAGTCTTCA ATGACTACAC AGCAGTTCCT GATCTCTACT TTGAGAACGC CATGCGCTTT 
TTCAACTTCT CATGGAGGGT CACAGCTGAC CAGCTCAGAA AAGCTCCCAA CAGAGATCAG 
TGGAGTATGA CGCCGCCCAT GGTGAACGCT TACTACTCGC CCACCAAGAA CGAGATCGTG 
TTTCCAGCTG GAATACTGCA GGCGCCATTT TATACCCGCT CTTCACCCAA CGCCTTGAAC 
TTCGGTGGCA TTGGTGTCGT AGTGGGCCAC GAGCTGACTC ACGCTTTCGA TGATCAAGGT 
CGGGAGTACG ACAAGGATGG GAACCTTCGA CCCTGGTGGA AGAACTCGTC CGTGGAGGCG 
TTCAAGCAGC AGACGGAGTG CATGGTGCAG CAATACAGCA ACTACAGCGT GAACGGGGAG 
CCCGTGAACG GTCGACACAC CCTCGGGGAG AACATTGCCG ACAACGGGGG ACTCAAGGCG 
GCCTACCGGG CGTACCAGAA CTGGGTAAAG AAGAACGGAG CTGAGCAGAC GCTGCCCACC 
CTGGGTCTCA CCAGCAACCA GCTCTTCTTC CTAGGATTTG CACAGGTCTG GTGCTCTGTC 
CGCACACCTG AGAGCTCCCA CGAAGGCCTC ATCACGGATC CGCACAGCCC CTCTCGCTTC 
CGGGTCATCG GCTCGCTCTC CAACTCCAAG GAGTTCTCAG AACACTTTCG CTGCCCGCCT 
GGCTCTCCCA TGAACCCTCA TCACAAGTGC GAAGTCTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006538830.1

Translation

MSTYKRATLDEEDLVDSLSEGDVYPNGLQVNFRSSRSGQRCWAARTSVEKRLVVLVTLLAAGLVACLAALGIQYQTRTPPVCLTEACVSVTSSILNSMDPTVDPCQDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAVIKHLLENATASVSEAERKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEDAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLRQVSTLINNTDKCLLNNYMMWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCIPRWKFCVSDTENNLGFALGPMFVKATFAEDSKNIASEIIMEIKKAFEESLSTLKWMDEETRRSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVQQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTSNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	XM_006538767.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus endothelin converting enzyme 1 (Ece1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538767.2

ORF Insert Sequence:

ATGTCAACCT ACAAGCGGGC CACGCTGGAC GAAGAGGATC TGGTGGACTC ACTCTCCGAG 
GGCGATGTGT ACCCCAATGG CCTACAGGTG AACTTCCGCA GCTCCCGGAG CGGCCAGAGG 
TGCTGGGCAG CTCGGACCTC GGTGGAGAAG CGGCTGGTGG TGCTGGTGAC GCTTCTGGCG 
GCAGGGTTGG TGGCCTGCCT GGCAGCCCTG GGCATCCAGT ACCAGACAAG AACGCCTCCG 
GTATGTCTGA CGGAGGCCTG TGTCTCAGTG ACCAGCTCTA TCCTAAACTC CATGGACCCC 
ACAGTAGACC CGTGCCAGGA CTTCTTCAGC TACGCCTGCG GGGGCTGGAT CAAGGCCAAC 
CCCGTGCCCG ATGGCCACTC ACGCTGGGGA ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCTGTCATCA AGCATCTACT GGAAAATGCC ACGGCCAGTG TGAGCGAGGC AGAGAGAAAA 
GCCCAAGTGT ACTACCGTGC GTGCATGAAC GAAACTAGGA TTGAGGAGCT TCGGGCCAAG 
CCCCTGATGG AGCTGATTGA GAAGCTGGGA GGCTGGAATA TCACAGGACC CTGGGCCAAG 
GACAACTTCC AGGACACACT GCAGGTGGTC ACAGCTCACT ACCGCACCTC ACCCTTCTTC 
TCTGTTTATG TCAGCGCGGA CTCCAAGAAT TCCAACAGCA ATGTGATCCA GGTGGACCAG 
TCCGGCCTTG GCTTGCCCTC CAGAGACTAT TACCTGAACA AGACAGAGAA TGAAAAGGTC 
CTGACTGGAT ACCTGAACTA CATGGTCCAG CTGGGGAAGC TCCTGGGCGG TGGGGACGAG 
GATGCCATCC GGCCACAGAT GCAGCAGATC CTGGATTTTG AGACGGCGCT GGCCAACATC 
ACCATCCCCC AGGAGAAGCG CAGGGATGAA GAACTCATCT ACCACAAAGT CACGGCTGCC 
GAGCTGCAGA CCTTGGCACC TGCCATCAAC TGGTTACCCT TTCTGAACAC CATTTTTTAC 
CCAGTGGAGA TCAATGAGTC TGAGCCAATC GTGGTCTACG ACAAGGAATA CTTGAGACAA 
GTCTCCACCC TCATCAACAA CACCGACAAA TGTCTGCTCA ACAACTACAT GATGTGGAAC 
CTGGTACGCA AAACAAGCTC CTTCCTGGAC CAGCGCTTTC AGGACGCCGA CGAGAAGTTC 
ATGGAGGTTA TGTATGGGAC GAAGAAGACC TGTATTCCCC GCTGGAAGTT TTGTGTGAGT 
GACACGGAAA ACAACCTGGG CTTTGCCCTG GGACCCATGT TTGTGAAAGC AACCTTTGCA 
GAAGACAGTA AGAATATAGC CAGTGAGATC ATCATGGAGA TCAAGAAGGC GTTTGAGGAG 
AGTCTGAGCA CTCTGAAATG GATGGATGAA GAGACTCGGA GATCAGCCAA GGAGAAGGCG 
GATGCTATCT ACAACATGAT AGGCTACCCC AACTTCATCA TGGACCCCAA GGAGCTGGAC 
AAAGTCTTCA ATGACTACAC AGCAGTTCCT GATCTCTACT TTGAGAACGC CATGCGCTTT 
TTCAACTTCT CATGGAGGGT CACAGCTGAC CAGCTCAGAA AAGCTCCCAA CAGAGATCAG 
TGGAGTATGA CGCCGCCCAT GGTGAACGCT TACTACTCGC CCACCAAGAA CGAGATCGTG 
TTTCCAGCTG GAATACTGCA GGCGCCATTT TATACCCGCT CTTCACCCAA CGCCTTGAAC 
TTCGGTGGCA TTGGTGTCGT AGTGGGCCAC GAGCTGACTC ACGCTTTCGA TGATCAAGGT 
CGGGAGTACG ACAAGGATGG GAACCTTCGA CCCTGGTGGA AGAACTCGTC CGTGGAGGCG 
TTCAAGCAGC AGACGGAGTG CATGGTGCAG CAATACAGCA ACTACAGCGT GAACGGGGAG 
CCCGTGAACG GTCGACACAC CCTCGGGGAG AACATTGCCG ACAACGGGGG ACTCAAGGCG 
GCCTACCGGG CGTACCAGAA CTGGGTAAAG AAGAACGGAG CTGAGCAGAC GCTGCCCACC 
CTGGGTCTCA CCAGCAACCA GCTCTTCTTC CTAGGATTTG CACAGGTCTG GTGCTCTGTC 
CGCACACCTG AGAGCTCCCA CGAAGGCCTC ATCACGGATC CGCACAGCCC CTCTCGCTTC 
CGGGTCATCG GCTCGCTCTC CAACTCCAAG GAGTTCTCAG AACACTTTCG CTGCCCGCCT 
GGCTCTCCCA TGAACCCTCA TCACAAGTGC GAAGTCTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spontaneous recombinase activity of Cre-ERT2 in vivo.
Transgenic research26(3)411-417(2017 Jun)
Kristianto J,Johnson MG,Zastrow RK,Radcliff AB,Blank RD

Nkx2.5 regulates endothelin converting enzyme-1 during pharyngeal arch patterning.
Genesis (New York, N.Y. : 2000)55(3)(2017 Mar)
Ikl? JM,Tavares AL,King M,Ding H,Colombo S,Firulli BA,Firulli AB,Targoff KL,Yelon D,Clouthier DE

Major amyloid-?-degrading enzymes, endothelin-converting enzyme-2 and neprilysin, are expressed by distinct populations of GABAergic interneurons in hippocampus and neocortex.
Neurobiology of aging4883-92(2016 Dec)
Pacheco-Quinto J,Eckman CB,Eckman EA

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Ece1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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