Aldob cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aldob
Entrez Gene ID	230163
Full Name	aldolase B, fructose-bisphosphate
Synonyms	Aldo-2,Aldo2,BC016435
General protein information	Preferred Names aldolase B, fructose-bisphosphate Names fructose-bisphosphate aldolase B aldolase 2, B isoform liver-type aldolase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 B1\|4 26.57 cM
Summary	This gene encodes a subunit of the homotetrameric enzyme aldolase B, an isozyme of the class I fructose 1,6-bisphosphate aldolase enzyme. This enzyme catalyzes the conversion of fructose 1,6-bisphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Homozygous knockout mice for this gene exhibit liver damage and death following fructose ingestion. A pseudogene of this gene has been identified in the genome. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_144903.3	NP_659152.1	fructose-bisphosphate aldolase B

Pathways from BioSystems

Homologies

Homo sapiens (human)	ALDOB	NP_000026.2
Gallus gallus (chicken)	ALDOB	NP_001007978.1
Canis lupus familiaris (dog)	ALDOB	XP_867333.3
Bos taurus (cattle)	ALDOB	NP_001029657.1
Mus musculus (house mouse)	Aldob	NP_659152.1
Rattus norvegicus (Norway rat)	Aldob	NP_036628.2
Danio rerio (zebrafish)	aldob	NP_919348.3
	ALDOB	XP_001111095.1
Pan troglodytes (chimpanzee)	ALDOB	XP_001136159.1
Xenopus tropicalis (tropical clawed frog)	aldob	NP_989131.1

Gene Ontology
Bibliography

Related articles in PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Rewiring of embryonic glucose metabolism via suppression of PFK-1 and aldolase during mouse chorioallantoic branching.
Miyazawa H, Yamaguchi Y, Sugiura Y, Honda K, Kondo K, Matsuda F, Yamamoto T, Suematsu M, Miura M
Development (Cambridge, England)144(1)63-73(2017 01)

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Bowman CE, Zhao L, Hartung T, Wolfgang MJ
Molecular and cellular biology36(15)2089-104(2016 Aug)

Aldolase-B knockout in mice phenocopies hereditary fructose intolerance in humans.
Oppelt SA, Sennott EM, Tolan DR
Molecular genetics and metabolism114(3)445-50(2015 Mar)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NKCC2 surface expression in mammalian cells is shown to be downregulated by a novel interaction with aldolase B
Title: NKCC2 surface expression in mammalian cells: down-regulation by novel interaction with aldolase B.

characterization of the aldolase B gene enhancer
Title: In vivo functional characterization of the aldolase B gene enhancer.

The following Aldob gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aldob cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14611	NM_144903.3 Latest version!	Mus musculus aldolase B, fructose-bisphosphate (Aldob), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aldob ( NM_144903.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_659152.1 Mus musculus aldolase B, fructose-bisphosphate (Aldob), mRNA.

Price & Availability↑

CloneID	OMu14611
Clone ID Related Accession (Same CDS sequence)	NM_144903.3
Accession Version	NM_144903.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	fructose-bisphosphate aldolase B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK168997.1, AK167566.1 and AL772310.27. On Aug 21, 2015 this sequence version replaced NM_144903.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK149395.1, AK167566.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCACC GATTTCCAGC CCTCACCCCA GAGCAGAAGA AGGAGCTCTC TGAAATTGCC 
CAGCGCATTG TTGCCAATGG GAAGGGCATC CTGGCTGCAG ATGAATCTGT GGGTACCATG 
GGAAACCGCC TGCAAAGGAT AAAGGTGGAG AACACCGAAG AGAACCGAAG GCAGTTCCGA 
GAACTCCTCT TTAGTGTGGA CAATTCCATT AGCCAGAGCA TCGGCGGAGT GATCCTTTTC 
CACGAGACCC TCTACCAGAA GGACAGCCAG GGAAATCTGT TCAGAAACGT TCTCAAGGAG 
AAGGGAATTG TGGTGGGCAT CAAGTTGGAC CAAGGAGGTG CCCCGCTTGC AGGAACAAAC 
AAGGAAACCA CCATTCAAGG GCTTGATGGC CTCTCTGAAC GCTGTGCTCA GTACAAGAAA 
GATGGAGTTG ACTTTGGGAA GTGGCGTGCT GTGTTGAGGA TTGCTGACCA GTGTCCTTCC 
AGCCTTGCTA TCCAAGAAAA TGCCAATGCT CTGGCTCGCT ATGCCAGCAT CTGTCAGCAG 
AATGGGCTGG TCCCTATTGT TGAGCCAGAG GTGCTTCCTG ACGGAGACCA TGACCTGGAG 
CACTGCCAGT ATGTTTCTGA GAAGGTCCTG GCTGCTGTCT ACAAGGCTCT CAATGATCAT 
CATGTTTACC TAGAAGGCAC CTTGCTAAAG CCAAACATGG TGACTGCTGG ACATGCCTGC 
ACGAAGAAGT ATACACCAGA GCAGGTGGCT ATGGCCACTG TCACAGCTCT CCACAGAACT 
GTTCCTGCAG CTGTTCCTGG TATCTGCTTT TTGTCTGGAG GTATGAGTGA GGAGGATGCT 
ACACTTAACC TCAATGCTAT CAACCGTTGC CCTCTACCAA GGCCCTGGAA ACTAAGCTTT 
TCATATGGAA GAGCCCTCCA GGCCAGTGCA TTGGCTGCCT GGGGTGGCAA GGCTGCAAAC 
AAGAAGGCAA CCCAGGAAGC TTTCATGAAG CGGGCTATGG CTAACTGCCA GGCGGCCCAA 
GGACAGTACG TTCACACAGG CTCTTCAGGT GCTGCTGCCA CCCAGTCGCT CTTCACAGCC 
TCCTACACCT ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659152.1
CDS	124..1218
Translation	MAHRFPALTPEQKKELSEIAQRIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFRELLFSVDNSISQSIGGVILFHETLYQKDSQGNLFRNVLKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLAIQENANALARYASICQQNGLVPIVEPEVLPDGDHDLEHCQYVSEKVLAAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDATLNLNAINRCPLPRPWKLSFSYGRALQASALAAWGGKAANKKATQEAFMKRAMANCQAAQGQYVHTGSSGAAATQSLFTASYTY

Target ORF information:

RefSeq Version	NM_144903.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus aldolase B, fructose-bisphosphate (Aldob), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144903.3

ORF Insert Sequence:

ATGGCTCACC GATTTCCAGC CCTCACCCCA GAGCAGAAGA AGGAGCTCTC TGAAATTGCC 
CAGCGCATTG TTGCCAATGG GAAGGGCATC CTGGCTGCAG ATGAATCTGT GGGTACCATG 
GGAAACCGCC TGCAAAGGAT AAAGGTGGAG AACACCGAAG AGAACCGAAG GCAGTTCCGA 
GAACTCCTCT TTAGTGTGGA CAATTCCATT AGCCAGAGCA TCGGCGGAGT GATCCTTTTC 
CACGAGACCC TCTACCAGAA GGACAGCCAG GGAAATCTGT TCAGAAACGT TCTCAAGGAG 
AAGGGAATTG TGGTGGGCAT CAAGTTGGAC CAAGGAGGTG CCCCGCTTGC AGGAACAAAC 
AAGGAAACCA CCATTCAAGG GCTTGATGGC CTCTCTGAAC GCTGTGCTCA GTACAAGAAA 
GATGGAGTTG ACTTTGGGAA GTGGCGTGCT GTGTTGAGGA TTGCTGACCA GTGTCCTTCC 
AGCCTTGCTA TCCAAGAAAA TGCCAATGCT CTGGCTCGCT ATGCCAGCAT CTGTCAGCAG 
AATGGGCTGG TCCCTATTGT TGAGCCAGAG GTGCTTCCTG ACGGAGACCA TGACCTGGAG 
CACTGCCAGT ATGTTTCTGA GAAGGTCCTG GCTGCTGTCT ACAAGGCTCT CAATGATCAT 
CATGTTTACC TAGAAGGCAC CTTGCTAAAG CCAAACATGG TGACTGCTGG ACATGCCTGC 
ACGAAGAAGT ATACACCAGA GCAGGTGGCT ATGGCCACTG TCACAGCTCT CCACAGAACT 
GTTCCTGCAG CTGTTCCTGG TATCTGCTTT TTGTCTGGAG GTATGAGTGA GGAGGATGCT 
ACACTTAACC TCAATGCTAT CAACCGTTGC CCTCTACCAA GGCCCTGGAA ACTAAGCTTT 
TCATATGGAA GAGCCCTCCA GGCCAGTGCA TTGGCTGCCT GGGGTGGCAA GGCTGCAAAC 
AAGAAGGCAA CCCAGGAAGC TTTCATGAAG CGGGCTATGG CTAACTGCCA GGCGGCCCAA 
GGACAGTACG TTCACACAGG CTCTTCAGGT GCTGCTGCCA CCCAGTCGCT CTTCACAGCC 
TCCTACACCT ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Rewiring of embryonic glucose metabolism via suppression of PFK-1 and aldolase during mouse chorioallantoic branching.
Development (Cambridge, England)144(1)63-73(2017 01)
Miyazawa H,Yamaguchi Y,Sugiura Y,Honda K,Kondo K,Matsuda F,Yamamoto T,Suematsu M,Miura M

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Molecular and cellular biology36(15)2089-104(2016 Aug)
Bowman CE,Zhao L,Hartung T,Wolfgang MJ

Aldolase-B knockout in mice phenocopies hereditary fructose intolerance in humans.
Molecular genetics and metabolism114(3)445-50(2015 Mar)
Oppelt SA,Sennott EM,Tolan DR

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

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Aldob cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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