Madd cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Madd
Entrez Gene ID	228355
Full Name	MAP-kinase activating death domain
Synonyms	9630059K23Rik,IG20
General protein information	Preferred Names MAP-kinase activating death domain Names MAP kinase-activating death domain protein Rab3 GDP/GTP exchange protein Rab3 GEP insulinoma-glucagonoma protein 20 madd rab3 GDP/GTP exchange factor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017317619.1	XP_017173108.1	MAP kinase-activating death domain protein isoform X1
XM_017317624.1	XP_017173113.1	MAP kinase-activating death domain protein isoform X5
XM_017317625.1	XP_017173114.1	MAP kinase-activating death domain protein isoform X6
XM_030250204.1	XP_030106064.1	MAP kinase-activating death domain protein isoform X1
XM_030250207.1	XP_030106067.1	MAP kinase-activating death domain protein isoform X3
XM_030250217.1	XP_030106077.1	MAP kinase-activating death domain protein isoform X11
XM_030250233.1	XP_030106093.1	MAP kinase-activating death domain protein isoform X22
XM_030250239.1	XP_030106099.1	MAP kinase-activating death domain protein isoform X26
XM_030250241.1	XP_030106101.1	MAP kinase-activating death domain protein isoform X28
XM_030250245.1	XP_030106105.1	MAP kinase-activating death domain protein isoform X31
XM_030250248.1	XP_030106108.1	MAP kinase-activating death domain protein isoform X34
XM_030250249.1	XP_030106109.1	MAP kinase-activating death domain protein isoform X35
XM_030250251.1	XP_030106111.1	MAP kinase-activating death domain protein isoform X36
XM_030250253.1	XP_030106113.1	MAP kinase-activating death domain protein isoform X37
NM_001177719.1	NP_001171190.1	MAP kinase-activating death domain protein isoform 10
XM_017317620.1	XP_017173109.1	MAP kinase-activating death domain protein isoform X2
XM_017317626.1	XP_017173115.1	MAP kinase-activating death domain protein isoform X7
XM_017317628.1	XP_017173117.1	MAP kinase-activating death domain protein isoform X9
XM_017317629.1	XP_017173118.1	MAP kinase-activating death domain protein isoform X10
XM_030250208.1	XP_030106068.1	MAP kinase-activating death domain protein isoform X4
XM_030250211.1	XP_030106071.1	MAP kinase-activating death domain protein isoform X7
XM_030250213.1	XP_030106073.1	MAP kinase-activating death domain protein isoform X8
XM_030250220.1	XP_030106080.1	MAP kinase-activating death domain protein isoform X14
XM_030250225.1	XP_030106085.1	MAP kinase-activating death domain protein isoform X19
XM_030250237.1	XP_030106097.1	MAP kinase-activating death domain protein isoform X24
XM_030250246.1	XP_030106106.1	MAP kinase-activating death domain protein isoform X32
NM_001177720.1	NP_001171191.1	MAP kinase-activating death domain protein isoform 11
XM_017317627.1	XP_017173116.1	MAP kinase-activating death domain protein isoform X8
XM_017317630.1	XP_017173119.1	MAP kinase-activating death domain protein isoform X11
XM_017317632.1	XP_017173121.1	MAP kinase-activating death domain protein isoform X12
XM_030250206.1	XP_030106066.1	MAP kinase-activating death domain protein isoform X2
XM_030250214.1	XP_030106074.1	MAP kinase-activating death domain protein isoform X9
XM_030250216.1	XP_030106076.1	MAP kinase-activating death domain protein isoform X10
XM_030250223.1	XP_030106083.1	MAP kinase-activating death domain protein isoform X17
XM_030250227.1	XP_030106087.1	MAP kinase-activating death domain protein isoform X20
XM_030250230.1	XP_030106090.1	MAP kinase-activating death domain protein isoform X21
XM_030250238.1	XP_030106098.1	MAP kinase-activating death domain protein isoform X25
XM_030250257.1	XP_030106117.1	MAP kinase-activating death domain protein isoform X38
NM_145527.4	NP_663502.3	MAP kinase-activating death domain protein isoform 9
XM_030250210.1	XP_030106070.1	MAP kinase-activating death domain protein isoform X6
XM_030250212.1	XP_030106072.1	MAP kinase-activating death domain protein isoform X8
XM_030250219.1	XP_030106079.1	MAP kinase-activating death domain protein isoform X13
XM_030250221.1	XP_030106081.1	MAP kinase-activating death domain protein isoform X15
XM_030250224.1	XP_030106084.1	MAP kinase-activating death domain protein isoform X18
XM_030250242.1	XP_030106102.1	MAP kinase-activating death domain protein isoform X29
XM_030250247.1	XP_030106107.1	MAP kinase-activating death domain protein isoform X33
XM_017317621.1	XP_017173110.1	MAP kinase-activating death domain protein isoform X3
XM_017317623.1	XP_017173112.1	MAP kinase-activating death domain protein isoform X4
XM_017317634.1	XP_017173123.1	MAP kinase-activating death domain protein isoform X13
XM_030250202.1	XP_030106062.1	MAP kinase-activating death domain protein isoform X1
XM_030250203.1	XP_030106063.1	MAP kinase-activating death domain protein isoform X1
XM_030250205.1	XP_030106065.1	MAP kinase-activating death domain protein isoform X1
XM_030250209.1	XP_030106069.1	MAP kinase-activating death domain protein isoform X5
XM_030250218.1	XP_030106078.1	MAP kinase-activating death domain protein isoform X12
XM_030250222.1	XP_030106082.1	MAP kinase-activating death domain protein isoform X16
XM_030250236.1	XP_030106096.1	MAP kinase-activating death domain protein isoform X23
XM_030250240.1	XP_030106100.1	MAP kinase-activating death domain protein isoform X27
XM_030250244.1	XP_030106104.1	MAP kinase-activating death domain protein isoform X30

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MADD	NP_001180007.1
Rattus norvegicus (Norway rat)	Madd	NP_446037.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000630	XP_311160.5
Caenorhabditis elegans (roundworm)	aex-3	NP_741710.1
Xenopus tropicalis (tropical clawed frog)	madd	NP_001072717.1
Mus musculus (house mouse)	Madd	NP_001171191.1
Danio rerio (zebrafish)	madd	XP_005169012.1
	MADD	XP_001107689.1
Homo sapiens (human)	MADD	NP_003673.3
Pan troglodytes (chimpanzee)	MADD	XP_003951918.1
Canis lupus familiaris (dog)	MADD	XP_005631300.1
Gallus gallus (chicken)	MADD	XP_004941623.1
Drosophila melanogaster (fruit fly)	rab3-GEF	NP_001096983.2

Gene Ontology
Bibliography

Related articles in PubMed

IG20/MADD plays a critical role in glucose-induced insulin secretion.
Li LC, Wang Y, Carr R, Haddad CS, Li Z, Qian L, Oberholzer J, Maker AV, Wang Q, Prabhakar BS
Diabetes63(5)1612-23(2014 May)

A mutation in the Srrm4 gene causes alternative splicing defects and deafness in the Bronx waltzer mouse.
Nakano Y, Jahan I, Bonde G, Sun X, Hildebrand MS, Engelhardt JF, Smith RJ, Cornell RA, Fritzsch B, B?nfi B
PLoS genetics8(10)e1002966(2012)

KIF1Bbeta- and KIF1A-mediated axonal transport of presynaptic regulator Rab3 occurs in a GTP-dependent manner through DENN/MADD.
Niwa S, Tanaka Y, Hirokawa N
Nature cell biology10(11)1269-79(2008 Nov)

Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.
Munton RP, Tweedie-Cullen R, Livingstone-Zatchej M, Weinandy F, Waidelich M, Longo D, Gehrig P, Potthast F, Rutishauser D, Gerrits B, Panse C, Schlapbach R, Mansuy IM
Molecular & cellular proteomics : MCP6(2)283-93(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings indicated that IG20/MADD plays a critical role in glucose-induced insulin release from beta-cells and that its functional disruption can cause type 2 diabetes.
Title: IG20/MADD plays a critical role in glucose-induced insulin secretion.

DENN has anti-apoptotic and cell survival roles in tumor cells
Title: Antisense abrogation of DENN expression induces apoptosis of leukemia cells in vitro, causes tumor regression in vivo and alters the transcription of genes involved in apoptosis and the cell cycle.

role of Rab3 GEP in regulating neurotransmitter release in autapses of mouse hippocampal neurons in culture
Title: A GDP/GTP exchange protein for the Rab3 small G protein family up-regulates a postdocking step of synaptic exocytosis in central synapses.

The following Madd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Madd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu73396	XM_030250257.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X42, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu11735	NM_145527.4 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$916.30 $1309.00
OMu00759	XM_030250212.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu00759	XM_030250213.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu11735	XM_017317628.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$916.30 $1309.00
OMu30279	XM_030250207.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu30283	XM_030250227.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu30287	XM_030250238.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu30288	XM_030250240.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu30289	XM_030250242.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu30291	XM_030250245.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu30292	XM_030250247.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu30293	XM_030250248.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu30294	XM_030250251.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu73390	XM_030250202.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu73390	XM_030250204.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu73390	XM_030250203.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu73390	XM_030250205.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu73391	XM_030250206.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu73392	XM_030250211.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu73393	XM_030250219.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu73394	XM_030250237.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu73395	XM_030250241.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87823	XM_030250208.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87824	XM_030250209.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87825	XM_030250210.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87826	XM_030250214.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87827	XM_030250216.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87828	XM_030250217.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87829	XM_030250218.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87830	XM_030250220.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87831	XM_030250221.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87832	XM_030250222.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87833	XM_030250223.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87834	XM_030250224.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87835	XM_030250225.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87836	XM_030250230.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87837	XM_030250233.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87838	XM_030250236.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87839	XM_030250239.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87840	XM_030250244.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87841	XM_030250246.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87842	XM_030250249.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu87843	XM_030250253.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu00757	NM_001177719.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OMu00759	NM_001177720.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$958.30 $1369.00
OMu00757	XM_017317626.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OMu00759	XM_017317620.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$958.30 $1369.00
OMu30279	XM_017317619.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu30283	XM_017317621.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu30287	XM_017317623.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu30288	XM_017317624.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu30289	XM_017317625.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu30291	XM_017317627.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu30292	XM_017317629.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu30293	XM_017317630.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu30294	XM_017317632.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu73396	XM_017317634.1 Latest version!	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Madd ( XM_030250257.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106117.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X42, mRNA.

Price & Availability↑

CloneID	OMu73396
Clone ID Related Accession (Same CDS sequence)	XM_030250257.1 , XM_017317634.1
Accession Version	XM_030250257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X38
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106117.1
CDS	337..4767
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_030250257.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X42, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250257.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( NM_145527.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663502.3 Mus musculus MAP-kinase activating death domain (Madd), transcript variant 9, mRNA.

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CloneID	OMu11735
Clone ID Related Accession (Same CDS sequence)	XM_017317628.1 , NM_145527.4
Accession Version	NM_145527.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform 9
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK036347.1 and BC063386.1. On Apr 25, 2010 this sequence version replaced NM_145527.3. Transcript Variant: This variant (9) lacks one exon and uses an alternate splice site in the coding region, compared to variant 10. The resulting isoform (9) differs at two internal portions, compared to isoform 10. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC063386.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663502.3
CDS	337..5013
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_145527.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145527.4

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250213.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106073.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X12, mRNA.

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CloneID	OMu00759
Clone ID Related Accession (Same CDS sequence)	XM_017317620.1 , XM_030250213.1 , NM_001177720.1 , XM_030250212.1
Accession Version	XM_030250213.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106073.1
CDS	283..5166
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250213.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250213.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250212.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106072.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X11, mRNA.

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CloneID	OMu00759
Clone ID Related Accession (Same CDS sequence)	XM_017317620.1 , XM_030250213.1 , NM_001177720.1 , XM_030250212.1
Accession Version	XM_030250212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106072.1
CDS	337..5220
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250212.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250212.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_017317628.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173117.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X12, mRNA.

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CloneID	OMu11735
Clone ID Related Accession (Same CDS sequence)	XM_017317628.1 , NM_145527.4
Accession Version	XM_017317628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173117.1
CDS	295..4971
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017317628.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317628.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250207.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106067.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu30279
Clone ID Related Accession (Same CDS sequence)	XM_017317619.1 , XM_030250207.1
Accession Version	XM_030250207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106067.1
CDS	337..5271
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250207.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250207.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250227.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106087.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X24, mRNA.

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CloneID	OMu30283
Clone ID Related Accession (Same CDS sequence)	XM_030250227.1 , XM_017317621.1
Accession Version	XM_030250227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAAG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCCCACG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC CGCAGTTCGT AGCAGTGAAG AAGACCTTCG AACCCCACCT 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106087.1
CDS	337..5154
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250227.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250227.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAAG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCCCACG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC CGCAGTTCGT AGCAGTGAAG AAGACCTTCG AACCCCACCT 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250238.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106098.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X29, mRNA.

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CloneID	OMu30287
Clone ID Related Accession (Same CDS sequence)	XM_030250238.1 , XM_017317623.1
Accession Version	XM_030250238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAAG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCCCACG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC CGCAGTTCGT AGCAGTGAAG AAGACCTTCG AACCCCACCT 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106098.1
CDS	337..5094
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250238.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250238.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAAG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCCCACG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC CGCAGTTCGT AGCAGTGAAG AAGACCTTCG AACCCCACCT 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250240.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106100.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X31, mRNA.

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CloneID	OMu30288
Clone ID Related Accession (Same CDS sequence)	XM_017317624.1 , XM_030250240.1
Accession Version	XM_030250240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGAGTAATA GTTCCGGAGA GACCCTTGGA GCTGACAGTG ACCTGAGCAG CAATGCAGGT 
GACGGGCCAG GAGGCGAAGG CAGCGCCCAC CTGGCAAGTT CCCGGGCCAC TCTGTCCGAC 
AGTGAAATCG AAACCAATTC TGCTACAAGC GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAAGGAGA AGCCGGCTGG TAGTCCGATT CGCTCTTCCG AAGACGTAAG CCAGCGAGTC 
TATCTCTATG AGGGACTGCT AGGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106100.1
CDS	337..5091
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250240.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250240.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGAGTAATA GTTCCGGAGA GACCCTTGGA GCTGACAGTG ACCTGAGCAG CAATGCAGGT 
GACGGGCCAG GAGGCGAAGG CAGCGCCCAC CTGGCAAGTT CCCGGGCCAC TCTGTCCGAC 
AGTGAAATCG AAACCAATTC TGCTACAAGC GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAAGGAGA AGCCGGCTGG TAGTCCGATT CGCTCTTCCG AAGACGTAAG CCAGCGAGTC 
TATCTCTATG AGGGACTGCT AGGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250242.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106102.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OMu30289
Clone ID Related Accession (Same CDS sequence)	XM_017317625.1 , XM_030250242.1
Accession Version	XM_030250242.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106102.1
CDS	337..5073
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_030250242.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250242.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250245.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106105.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X35, mRNA.

Price & Availability↑

CloneID	OMu30291
Clone ID Related Accession (Same CDS sequence)	XM_030250245.1 , XM_017317627.1
Accession Version	XM_030250245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GTATTTGGGC TAAATACTCT AATGGAGATT GTTACTGAAG CCGGCCCTGG GAGTGGTGAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTTC GAGGCGTCTT TGTCCTGGAA GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC AATGGCCCAC GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CCGCAGTTCG TAGCAGTGAA GAAGACCTTC GAACCCCACC TCGGCCTGTC 
TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106105.1
CDS	337..5025
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250245.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250245.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GTATTTGGGC TAAATACTCT AATGGAGATT GTTACTGAAG CCGGCCCTGG GAGTGGTGAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTTC GAGGCGTCTT TGTCCTGGAA GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC AATGGCCCAC GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CCGCAGTTCG TAGCAGTGAA GAAGACCTTC GAACCCCACC TCGGCCTGTC 
TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250247.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106107.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X37, mRNA.

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CloneID	OMu30292
Clone ID Related Accession (Same CDS sequence)	XM_017317629.1 , XM_030250247.1
Accession Version	XM_030250247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTTC GAGGCGTCTT TGTCCTGGAA GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC AATGGCCCAC GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CCGCAGTTCG TAGCAGTGAA GAAGACCTTC GAACCCCACC TCGGCCTGTC 
TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106107.1
CDS	337..4965
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250247.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250247.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTTC GAGGCGTCTT TGTCCTGGAA GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC AATGGCCCAC GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CCGCAGTTCG TAGCAGTGAA GAAGACCTTC GAACCCCACC TCGGCCTGTC 
TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250248.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106108.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X38, mRNA.

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CloneID	OMu30293
Clone ID Related Accession (Same CDS sequence)	XM_030250248.1 , XM_017317630.1
Accession Version	XM_030250248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGAGTAAT 
AGTTCCGGAG AGACCCTTGG AGCTGACAGT GACCTGAGCA GCAATGCAGG TGACGGGCCA 
GGAGGCGAAG GCAGCGCCCA CCTGGCAAGT TCCCGGGCCA CTCTGTCCGA CAGTGAAATC 
GAAACCAATT CTGCTACAAG CGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAAGGAG 
AAGCCGGCTG GTAGTCCGAT TCGCTCTTCC GAAGACGTAA GCCAGCGAGT CTATCTCTAT 
GAGGGACTGC TAGGCAAAGA ACGTTCTACC TTATGGGACC AAATGCAGTT CTGGGAAGAT 
GCATTCTTAG ATGCTGTAAT GTTGGAAAGA GAAGGAATGG GTATGGACCA GGGTCCTCAG 
GAAATGATCG ACAGGTACCT CTCCCTAGGG GAACACGACC GGAAGCGCCT GGAGGATGAT 
GAAGACCGCT TGCTGGCCAC ACTTTTACAC AACCTCATAT CCTATATGCT GCTGATGAAG 
GTGAACAAGA ATGACATCAG GAAGAAAGTA CGGCGCCTAA TGGGAAAGTC CCACATTGGG 
CTGGTGTACA GCCAGCAAGT CAATGAGGTG CTCGATCAGC TGAACAGCCT GAATGGACGT 
GATCTCTCCA TCCGGTCCAG CGGCAGCCGG CACATGAAGA AGCAGACTTT TGTTGTGCAT 
GCAGGGACGG ACACAAATGG AGACATCTTT TTCATGGAAG TGTGTGATGA CTGCGTGGTG 
TTGCGTAGTA ACATTGGGAC GGTGTACGAG CGCTGGTGGT ATGAGAAGCT CATCAATATG 
ACCTACTGTC CCAAGACCAA GGTCCTGTGC TTATGGCGGA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACCAA GAAGTGTCGA GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAACGTGCTG CTGCCCGACA GCAAAGCATC AAACCTGGGC CTGAGCTAGG CGGTGAGTTC 
CCTGTGCAGG ACATGAAGAC TGGAGAGGGT GGCTTGCTTC AAGTCACCCT GGAAGGGATC 
AATCTCAAGT TCATGCACAA CCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAAGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACGCCAAT GGCCCACGAG ATCTGCTACT CTGTGTTGTG TCTCTTCTCG 
TATGTGGCCG CAGTTCGTAG CAGTGAAGAA GACCTTCGAA CCCCACCTCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106108.1
CDS	337..4962
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250248.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250248.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGAGTAAT 
AGTTCCGGAG AGACCCTTGG AGCTGACAGT GACCTGAGCA GCAATGCAGG TGACGGGCCA 
GGAGGCGAAG GCAGCGCCCA CCTGGCAAGT TCCCGGGCCA CTCTGTCCGA CAGTGAAATC 
GAAACCAATT CTGCTACAAG CGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAAGGAG 
AAGCCGGCTG GTAGTCCGAT TCGCTCTTCC GAAGACGTAA GCCAGCGAGT CTATCTCTAT 
GAGGGACTGC TAGGCAAAGA ACGTTCTACC TTATGGGACC AAATGCAGTT CTGGGAAGAT 
GCATTCTTAG ATGCTGTAAT GTTGGAAAGA GAAGGAATGG GTATGGACCA GGGTCCTCAG 
GAAATGATCG ACAGGTACCT CTCCCTAGGG GAACACGACC GGAAGCGCCT GGAGGATGAT 
GAAGACCGCT TGCTGGCCAC ACTTTTACAC AACCTCATAT CCTATATGCT GCTGATGAAG 
GTGAACAAGA ATGACATCAG GAAGAAAGTA CGGCGCCTAA TGGGAAAGTC CCACATTGGG 
CTGGTGTACA GCCAGCAAGT CAATGAGGTG CTCGATCAGC TGAACAGCCT GAATGGACGT 
GATCTCTCCA TCCGGTCCAG CGGCAGCCGG CACATGAAGA AGCAGACTTT TGTTGTGCAT 
GCAGGGACGG ACACAAATGG AGACATCTTT TTCATGGAAG TGTGTGATGA CTGCGTGGTG 
TTGCGTAGTA ACATTGGGAC GGTGTACGAG CGCTGGTGGT ATGAGAAGCT CATCAATATG 
ACCTACTGTC CCAAGACCAA GGTCCTGTGC TTATGGCGGA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACCAA GAAGTGTCGA GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAACGTGCTG CTGCCCGACA GCAAAGCATC AAACCTGGGC CTGAGCTAGG CGGTGAGTTC 
CCTGTGCAGG ACATGAAGAC TGGAGAGGGT GGCTTGCTTC AAGTCACCCT GGAAGGGATC 
AATCTCAAGT TCATGCACAA CCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAAGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACGCCAAT GGCCCACGAG ATCTGCTACT CTGTGTTGTG TCTCTTCTCG 
TATGTGGCCG CAGTTCGTAG CAGTGAAGAA GACCTTCGAA CCCCACCTCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250251.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106111.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X40, mRNA.

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CloneID	OMu30294
Clone ID Related Accession (Same CDS sequence)	XM_030250251.1 , XM_017317632.1
Accession Version	XM_030250251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTGAGTAA TAGTTCCGGA 
GAGACCCTTG GAGCTGACAG TGACCTGAGC AGCAATGCAG GTGACGGGCC AGGAGGCGAA 
GGCAGCGCCC ACCTGGCAAG TTCCCGGGCC ACTCTGTCCG ACAGTGAAAT CGAAACCAAT 
TCTGCTACAA GCGCCATCTT TGGTAAAGCC CATAGCTTGA AGCCAAAGGA GAAGCCGGCT 
GGTAGTCCGA TTCGCTCTTC CGAAGACGTA AGCCAGCGAG TCTATCTCTA TGAGGGACTG 
CTAGGCAAAG AACGTTCTAC CTTATGGGAC CAAATGCAGT TCTGGGAAGA TGCATTCTTA 
GATGCTGTAA TGTTGGAAAG AGAAGGAATG GGTATGGACC AGGGTCCTCA GGAAATGATC 
GACAGGTACC TCTCCCTAGG GGAACACGAC CGGAAGCGCC TGGAGGATGA TGAAGACCGC 
TTGCTGGCCA CACTTTTACA CAACCTCATA TCCTATATGC TGCTGATGAA GGTGAACAAG 
AATGACATCA GGAAGAAAGT ACGGCGCCTA ATGGGAAAGT CCCACATTGG GCTGGTGTAC 
AGCCAGCAAG TCAATGAGGT GCTCGATCAG CTGAACAGCC TGAATGGACG TGATCTCTCC 
ATCCGGTCCA GCGGCAGCCG GCACATGAAG AAGCAGACTT TTGTTGTGCA TGCAGGGACG 
GACACAAATG GAGACATCTT TTTCATGGAA GTGTGTGATG ACTGCGTGGT GTTGCGTAGT 
AACATTGGGA CGGTGTACGA GCGCTGGTGG TATGAGAAGC TCATCAATAT GACCTACTGT 
CCCAAGACCA AGGTCCTGTG CTTATGGCGG AGAAATGGCT CTGAGACCCA GCTCAACAAG 
TTCTATACCA AGAAGTGTCG AGAGCTGTAC TACTGTGTGA AGGACAGCAT GGAACGTGCT 
GCTGCCCGAC AGCAAAGCAT CAAACCTGGG CCTGAGCTAG GCGGTGAGTT CCCTGTGCAG 
GACATGAAGA CTGGAGAGGG TGGCTTGCTT CAAGTCACCC TGGAAGGGAT CAATCTCAAG 
TTCATGCACA ACCAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTTCGA 
GGCGTCTTTG TCCTGGAAGA ATTTGTTCCT GAAATTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCCCACGA GATCTGCTAC TCTGTGTTGT GTCTCTTCTC GTATGTGGCC 
GCAGTTCGTA GCAGTGAAGA AGACCTTCGA ACCCCACCTC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106111.1
CDS	337..4953
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250251.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250251.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTGAGTAA TAGTTCCGGA 
GAGACCCTTG GAGCTGACAG TGACCTGAGC AGCAATGCAG GTGACGGGCC AGGAGGCGAA 
GGCAGCGCCC ACCTGGCAAG TTCCCGGGCC ACTCTGTCCG ACAGTGAAAT CGAAACCAAT 
TCTGCTACAA GCGCCATCTT TGGTAAAGCC CATAGCTTGA AGCCAAAGGA GAAGCCGGCT 
GGTAGTCCGA TTCGCTCTTC CGAAGACGTA AGCCAGCGAG TCTATCTCTA TGAGGGACTG 
CTAGGCAAAG AACGTTCTAC CTTATGGGAC CAAATGCAGT TCTGGGAAGA TGCATTCTTA 
GATGCTGTAA TGTTGGAAAG AGAAGGAATG GGTATGGACC AGGGTCCTCA GGAAATGATC 
GACAGGTACC TCTCCCTAGG GGAACACGAC CGGAAGCGCC TGGAGGATGA TGAAGACCGC 
TTGCTGGCCA CACTTTTACA CAACCTCATA TCCTATATGC TGCTGATGAA GGTGAACAAG 
AATGACATCA GGAAGAAAGT ACGGCGCCTA ATGGGAAAGT CCCACATTGG GCTGGTGTAC 
AGCCAGCAAG TCAATGAGGT GCTCGATCAG CTGAACAGCC TGAATGGACG TGATCTCTCC 
ATCCGGTCCA GCGGCAGCCG GCACATGAAG AAGCAGACTT TTGTTGTGCA TGCAGGGACG 
GACACAAATG GAGACATCTT TTTCATGGAA GTGTGTGATG ACTGCGTGGT GTTGCGTAGT 
AACATTGGGA CGGTGTACGA GCGCTGGTGG TATGAGAAGC TCATCAATAT GACCTACTGT 
CCCAAGACCA AGGTCCTGTG CTTATGGCGG AGAAATGGCT CTGAGACCCA GCTCAACAAG 
TTCTATACCA AGAAGTGTCG AGAGCTGTAC TACTGTGTGA AGGACAGCAT GGAACGTGCT 
GCTGCCCGAC AGCAAAGCAT CAAACCTGGG CCTGAGCTAG GCGGTGAGTT CCCTGTGCAG 
GACATGAAGA CTGGAGAGGG TGGCTTGCTT CAAGTCACCC TGGAAGGGAT CAATCTCAAG 
TTCATGCACA ACCAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTTCGA 
GGCGTCTTTG TCCTGGAAGA ATTTGTTCCT GAAATTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCCCACGA GATCTGCTAC TCTGTGTTGT GTCTCTTCTC GTATGTGGCC 
GCAGTTCGTA GCAGTGAAGA AGACCTTCGA ACCCCACCTC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250204.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106064.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X3, mRNA.

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CloneID	OMu73390
Clone ID Related Accession (Same CDS sequence)	XM_030250204.1 , XM_030250202.1 , XM_030250203.1 , XM_030250205.1
Accession Version	XM_030250204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106064.1
CDS	343..5286
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250204.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250204.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250202.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106062.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73390
Clone ID Related Accession (Same CDS sequence)	XM_030250204.1 , XM_030250202.1 , XM_030250203.1 , XM_030250205.1
Accession Version	XM_030250202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106062.1
CDS	337..5280
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250202.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250202.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250203.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106063.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X2, mRNA.

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CloneID	OMu73390
Clone ID Related Accession (Same CDS sequence)	XM_030250204.1 , XM_030250202.1 , XM_030250203.1 , XM_030250205.1
Accession Version	XM_030250203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106063.1
CDS	283..5226
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250203.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250203.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250205.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106065.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X4, mRNA.

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CloneID	OMu73390
Clone ID Related Accession (Same CDS sequence)	XM_030250204.1 , XM_030250202.1 , XM_030250203.1 , XM_030250205.1
Accession Version	XM_030250205.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106065.1
CDS	159..5102
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250205.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250205.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250206.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106066.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X5, mRNA.

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CloneID	OMu73391
Clone ID Related Accession (Same CDS sequence)	XM_030250206.1
Accession Version	XM_030250206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGCTAGA GGATGCCGCT 
ATGGAGACCT TTTCTCTGAG CAAAGAACGT TCTACCTTAT GGGACCAAAT GCAGTTCTGG 
GAAGATGCAT TCTTAGATGC TGTAATGTTG GAAAGAGAAG GAATGGGTAT GGACCAGGGT 
CCTCAGGAAA TGATCGACAG GTACCTCTCC CTAGGGGAAC ACGACCGGAA GCGCCTGGAG 
GATGATGAAG ACCGCTTGCT GGCCACACTT TTACACAACC TCATATCCTA TATGCTGCTG 
ATGAAGGTGA ACAAGAATGA CATCAGGAAG AAAGTACGGC GCCTAATGGG AAAGTCCCAC 
ATTGGGCTGG TGTACAGCCA GCAAGTCAAT GAGGTGCTCG ATCAGCTGAA CAGCCTGAAT 
GGACGTGATC TCTCCATCCG GTCCAGCGGC AGCCGGCACA TGAAGAAGCA GACTTTTGTT 
GTGCATGCAG GGACGGACAC AAATGGAGAC ATCTTTTTCA TGGAAGTGTG TGATGACTGC 
GTGGTGTTGC GTAGTAACAT TGGGACGGTG TACGAGCGCT GGTGGTATGA GAAGCTCATC 
AATATGACCT ACTGTCCCAA GACCAAGGTC CTGTGCTTAT GGCGGAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACCAAGAAG TGTCGAGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAAC GTGCTGCTGC CCGACAGCAA AGCATCAAAC CTGGGCCTGA GCTAGGCGGT 
GAGTTCCCTG TGCAGGACAT GAAGACTGGA GAGGGTGGCT TGCTTCAAGT CACCCTGGAA 
GGGATCAATC TCAAGTTCAT GCACAACCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AAGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCCC ACGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCCGCAGTT CGTAGCAGTG AAGAAGACCT TCGAACCCCA 
CCTCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106066.1
CDS	337..5277
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250206.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250206.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGCTAGA GGATGCCGCT 
ATGGAGACCT TTTCTCTGAG CAAAGAACGT TCTACCTTAT GGGACCAAAT GCAGTTCTGG 
GAAGATGCAT TCTTAGATGC TGTAATGTTG GAAAGAGAAG GAATGGGTAT GGACCAGGGT 
CCTCAGGAAA TGATCGACAG GTACCTCTCC CTAGGGGAAC ACGACCGGAA GCGCCTGGAG 
GATGATGAAG ACCGCTTGCT GGCCACACTT TTACACAACC TCATATCCTA TATGCTGCTG 
ATGAAGGTGA ACAAGAATGA CATCAGGAAG AAAGTACGGC GCCTAATGGG AAAGTCCCAC 
ATTGGGCTGG TGTACAGCCA GCAAGTCAAT GAGGTGCTCG ATCAGCTGAA CAGCCTGAAT 
GGACGTGATC TCTCCATCCG GTCCAGCGGC AGCCGGCACA TGAAGAAGCA GACTTTTGTT 
GTGCATGCAG GGACGGACAC AAATGGAGAC ATCTTTTTCA TGGAAGTGTG TGATGACTGC 
GTGGTGTTGC GTAGTAACAT TGGGACGGTG TACGAGCGCT GGTGGTATGA GAAGCTCATC 
AATATGACCT ACTGTCCCAA GACCAAGGTC CTGTGCTTAT GGCGGAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACCAAGAAG TGTCGAGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAAC GTGCTGCTGC CCGACAGCAA AGCATCAAAC CTGGGCCTGA GCTAGGCGGT 
GAGTTCCCTG TGCAGGACAT GAAGACTGGA GAGGGTGGCT TGCTTCAAGT CACCCTGGAA 
GGGATCAATC TCAAGTTCAT GCACAACCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AAGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCCC ACGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCCGCAGTT CGTAGCAGTG AAGAAGACCT TCGAACCCCA 
CCTCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250211.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106071.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X10, mRNA.

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CloneID	OMu73392
Clone ID Related Accession (Same CDS sequence)	XM_030250211.1
Accession Version	XM_030250211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGAGTAATA GTTCCGGAGA GACCCTTGGA GCTGACAGTG ACCTGAGCAG CAATGCAGGT 
GACGGGCCAG GAGGCGAAGG CAGCGCCCAC CTGGCAAGTT CCCGGGCCAC TCTGTCCGAC 
AGTGAAATCG AAACCAATTC TGCTACAAGC GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAAGGAGA AGCCGGCTGG TAGTCCGATT CGCTCTTCCG AAGACGTAAG CCAGCGAGTC 
TATCTCTATG AGGGACTGCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GCTAGAGGAT 
GCCGCTATGG AGACCTTTTC TCTGAGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAAGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACGCCAA TGGCCCACGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCC GCAGTTCGTA GCAGTGAAGA AGACCTTCGA 
ACCCCACCTC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106071.1
CDS	337..5223
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250211.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250211.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGAGTAATA GTTCCGGAGA GACCCTTGGA GCTGACAGTG ACCTGAGCAG CAATGCAGGT 
GACGGGCCAG GAGGCGAAGG CAGCGCCCAC CTGGCAAGTT CCCGGGCCAC TCTGTCCGAC 
AGTGAAATCG AAACCAATTC TGCTACAAGC GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAAGGAGA AGCCGGCTGG TAGTCCGATT CGCTCTTCCG AAGACGTAAG CCAGCGAGTC 
TATCTCTATG AGGGACTGCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GCTAGAGGAT 
GCCGCTATGG AGACCTTTTC TCTGAGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAAGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACGCCAA TGGCCCACGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCC GCAGTTCGTA GCAGTGAAGA AGACCTTCGA 
ACCCCACCTC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250219.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106079.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OMu73393
Clone ID Related Accession (Same CDS sequence)	XM_030250219.1
Accession Version	XM_030250219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAAG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCCCACG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC CGCAGTTCGT AGCAGTGAAG AAGACCTTCG AACCCCACCT 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106079.1
CDS	337..5214
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250219.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250219.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAAG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCCCACG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC CGCAGTTCGT AGCAGTGAAG AAGACCTTCG AACCCCACCT 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250237.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106097.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X28, mRNA.

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CloneID	OMu73394
Clone ID Related Accession (Same CDS sequence)	XM_030250237.1
Accession Version	XM_030250237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAAGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACGCCAA TGGCCCACGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCC GCAGTTCGTA GCAGTGAAGA AGACCTTCGA 
ACCCCACCTC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106097.1
CDS	337..5103
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250237.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250237.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAAGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACGCCAA TGGCCCACGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCC GCAGTTCGTA GCAGTGAAGA AGACCTTCGA 
ACCCCACCTC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250241.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106101.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X32, mRNA.

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CloneID	OMu73395
Clone ID Related Accession (Same CDS sequence)	XM_030250241.1
Accession Version	XM_030250241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTGAGTAAT 
AGTTCCGGAG AGACCCTTGG AGCTGACAGT GACCTGAGCA GCAATGCAGG TGACGGGCCA 
GGAGGCGAAG GCAGCGCCCA CCTGGCAAGT TCCCGGGCCA CTCTGTCCGA CAGTGAAATC 
GAAACCAATT CTGCTACAAG CGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAAGGAG 
AAGCCGGCTG GTAGTCCGAT TCGCTCTTCC GAAGACGTAA GCCAGCGAGT CTATCTCTAT 
GAGGGACTGC TAGGCAAAGA ACGTTCTACC TTATGGGACC AAATGCAGTT CTGGGAAGAT 
GCATTCTTAG ATGCTGTAAT GTTGGAAAGA GAAGGAATGG GTATGGACCA GGGTCCTCAG 
GAAATGATCG ACAGGTACCT CTCCCTAGGG GAACACGACC GGAAGCGCCT GGAGGATGAT 
GAAGACCGCT TGCTGGCCAC ACTTTTACAC AACCTCATAT CCTATATGCT GCTGATGAAG 
GTGAACAAGA ATGACATCAG GAAGAAAGTA CGGCGCCTAA TGGGAAAGTC CCACATTGGG 
CTGGTGTACA GCCAGCAAGT CAATGAGGTG CTCGATCAGC TGAACAGCCT GAATGGACGT 
GATCTCTCCA TCCGGTCCAG CGGCAGCCGG CACATGAAGA AGCAGACTTT TGTTGTGCAT 
GCAGGGACGG ACACAAATGG AGACATCTTT TTCATGGAAG TGTGTGATGA CTGCGTGGTG 
TTGCGTAGTA ACATTGGGAC GGTGTACGAG CGCTGGTGGT ATGAGAAGCT CATCAATATG 
ACCTACTGTC CCAAGACCAA GGTCCTGTGC TTATGGCGGA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACCAA GAAGTGTCGA GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAACGTGCTG CTGCCCGACA GCAAAGCATC AAACCTGGGC CTGAGCTAGG CGGTGAGTTC 
CCTGTGCAGG ACATGAAGAC TGGAGAGGGT GGCTTGCTTC AAGTCACCCT GGAAGGGATC 
AATCTCAAGT TCATGCACAA CCAGGAGCGG AAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106101.1
CDS	337..5091
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250241.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250241.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTGAGTAAT 
AGTTCCGGAG AGACCCTTGG AGCTGACAGT GACCTGAGCA GCAATGCAGG TGACGGGCCA 
GGAGGCGAAG GCAGCGCCCA CCTGGCAAGT TCCCGGGCCA CTCTGTCCGA CAGTGAAATC 
GAAACCAATT CTGCTACAAG CGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAAGGAG 
AAGCCGGCTG GTAGTCCGAT TCGCTCTTCC GAAGACGTAA GCCAGCGAGT CTATCTCTAT 
GAGGGACTGC TAGGCAAAGA ACGTTCTACC TTATGGGACC AAATGCAGTT CTGGGAAGAT 
GCATTCTTAG ATGCTGTAAT GTTGGAAAGA GAAGGAATGG GTATGGACCA GGGTCCTCAG 
GAAATGATCG ACAGGTACCT CTCCCTAGGG GAACACGACC GGAAGCGCCT GGAGGATGAT 
GAAGACCGCT TGCTGGCCAC ACTTTTACAC AACCTCATAT CCTATATGCT GCTGATGAAG 
GTGAACAAGA ATGACATCAG GAAGAAAGTA CGGCGCCTAA TGGGAAAGTC CCACATTGGG 
CTGGTGTACA GCCAGCAAGT CAATGAGGTG CTCGATCAGC TGAACAGCCT GAATGGACGT 
GATCTCTCCA TCCGGTCCAG CGGCAGCCGG CACATGAAGA AGCAGACTTT TGTTGTGCAT 
GCAGGGACGG ACACAAATGG AGACATCTTT TTCATGGAAG TGTGTGATGA CTGCGTGGTG 
TTGCGTAGTA ACATTGGGAC GGTGTACGAG CGCTGGTGGT ATGAGAAGCT CATCAATATG 
ACCTACTGTC CCAAGACCAA GGTCCTGTGC TTATGGCGGA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACCAA GAAGTGTCGA GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAACGTGCTG CTGCCCGACA GCAAAGCATC AAACCTGGGC CTGAGCTAGG CGGTGAGTTC 
CCTGTGCAGG ACATGAAGAC TGGAGAGGGT GGCTTGCTTC AAGTCACCCT GGAAGGGATC 
AATCTCAAGT TCATGCACAA CCAGGAGCGG AAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250208.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106068.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X7, mRNA.

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CloneID	OMu87823
Clone ID Related Accession (Same CDS sequence)	XM_030250208.1
Accession Version	XM_030250208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGTTCC 
GGAGAGACCC TTGGAGCTGA CAGTGACCTG AGCAGCAATG CAGGTGACGG GCCAGGAGGC 
GAAGGCAGCG CCCACCTGGC AAGTTCCCGG GCCACTCTGT CCGACAGTGA AATCGAAACC 
AATTCTGCTA CAAGCGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAA GGAGAAGCCG 
GCTGGTAGTC CGATTCGCTC TTCCGAAGAC GTAAGCCAGC GAGTCTATCT CTATGAGGGA 
CTGCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGCTAG AGGATGCCGC TATGGAGACC 
TTTTCTCTGA GCAAAGAACG TTCTACCTTA TGGGACCAAA TGCAGTTCTG GGAAGATGCA 
TTCTTAGATG CTGTAATGTT GGAAAGAGAA GGAATGGGTA TGGACCAGGG TCCTCAGGAA 
ATGATCGACA GGTACCTCTC CCTAGGGGAA CACGACCGGA AGCGCCTGGA GGATGATGAA 
GACCGCTTGC TGGCCACACT TTTACACAAC CTCATATCCT ATATGCTGCT GATGAAGGTG 
AACAAGAATG ACATCAGGAA GAAAGTACGG CGCCTAATGG GAAAGTCCCA CATTGGGCTG 
GTGTACAGCC AGCAAGTCAA TGAGGTGCTC GATCAGCTGA ACAGCCTGAA TGGACGTGAT 
CTCTCCATCC GGTCCAGCGG CAGCCGGCAC ATGAAGAAGC AGACTTTTGT TGTGCATGCA 
GGGACGGACA CAAATGGAGA CATCTTTTTC ATGGAAGTGT GTGATGACTG CGTGGTGTTG 
CGTAGTAACA TTGGGACGGT GTACGAGCGC TGGTGGTATG AGAAGCTCAT CAATATGACC 
TACTGTCCCA AGACCAAGGT CCTGTGCTTA TGGCGGAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACCAAGAA GTGTCGAGAG CTGTACTACT GTGTGAAGGA CAGCATGGAA 
CGTGCTGCTG CCCGACAGCA AAGCATCAAA CCTGGGCCTG AGCTAGGCGG TGAGTTCCCT 
GTGCAGGACA TGAAGACTGG AGAGGGTGGC TTGCTTCAAG TCACCCTGGA AGGGATCAAT 
CTCAAGTTCA TGCACAACCA GGAGCGGAAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TTCGAGGCGT CTTTGTCCTG GAAGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCC CACGAGATCT GCTACTCTGT GTTGTGTCTC 
TTCTCGTATG TGGCCGCAGT TCGTAGCAGT GAAGAAGACC TTCGAACCCC ACCTCGGCCT 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106068.1
CDS	337..5268
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250208.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250208.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGTTCC 
GGAGAGACCC TTGGAGCTGA CAGTGACCTG AGCAGCAATG CAGGTGACGG GCCAGGAGGC 
GAAGGCAGCG CCCACCTGGC AAGTTCCCGG GCCACTCTGT CCGACAGTGA AATCGAAACC 
AATTCTGCTA CAAGCGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAA GGAGAAGCCG 
GCTGGTAGTC CGATTCGCTC TTCCGAAGAC GTAAGCCAGC GAGTCTATCT CTATGAGGGA 
CTGCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGCTAG AGGATGCCGC TATGGAGACC 
TTTTCTCTGA GCAAAGAACG TTCTACCTTA TGGGACCAAA TGCAGTTCTG GGAAGATGCA 
TTCTTAGATG CTGTAATGTT GGAAAGAGAA GGAATGGGTA TGGACCAGGG TCCTCAGGAA 
ATGATCGACA GGTACCTCTC CCTAGGGGAA CACGACCGGA AGCGCCTGGA GGATGATGAA 
GACCGCTTGC TGGCCACACT TTTACACAAC CTCATATCCT ATATGCTGCT GATGAAGGTG 
AACAAGAATG ACATCAGGAA GAAAGTACGG CGCCTAATGG GAAAGTCCCA CATTGGGCTG 
GTGTACAGCC AGCAAGTCAA TGAGGTGCTC GATCAGCTGA ACAGCCTGAA TGGACGTGAT 
CTCTCCATCC GGTCCAGCGG CAGCCGGCAC ATGAAGAAGC AGACTTTTGT TGTGCATGCA 
GGGACGGACA CAAATGGAGA CATCTTTTTC ATGGAAGTGT GTGATGACTG CGTGGTGTTG 
CGTAGTAACA TTGGGACGGT GTACGAGCGC TGGTGGTATG AGAAGCTCAT CAATATGACC 
TACTGTCCCA AGACCAAGGT CCTGTGCTTA TGGCGGAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACCAAGAA GTGTCGAGAG CTGTACTACT GTGTGAAGGA CAGCATGGAA 
CGTGCTGCTG CCCGACAGCA AAGCATCAAA CCTGGGCCTG AGCTAGGCGG TGAGTTCCCT 
GTGCAGGACA TGAAGACTGG AGAGGGTGGC TTGCTTCAAG TCACCCTGGA AGGGATCAAT 
CTCAAGTTCA TGCACAACCA GGAGCGGAAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TTCGAGGCGT CTTTGTCCTG GAAGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCC CACGAGATCT GCTACTCTGT GTTGTGTCTC 
TTCTCGTATG TGGCCGCAGT TCGTAGCAGT GAAGAAGACC TTCGAACCCC ACCTCGGCCT 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250209.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106069.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X8, mRNA.

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CloneID	OMu87824
Clone ID Related Accession (Same CDS sequence)	XM_030250209.1
Accession Version	XM_030250209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGTTCC 
GGAGAGACCC TTGGAGCTGA CAGTGACCTG AGCAGCAATG CAGGTGACGG GCCAGGAGGC 
GAAGGCAGCG CCCACCTGGC AAGTTCCCGG GCCACTCTGT CCGACAGTGA AATCGAAACC 
AATTCTGCTA CAAGCGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAA GGAGAAGCCG 
GCTGGTAGTC CGATTCGCTC TTCCGAAGAC GTAAGCCAGC GAGTCTATCT CTATGAGGGA 
CTGCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGCTAG AGGATGCCGC TATGGAGACC 
TTTTCTCTGA GCAAAGAACG TTCTACCTTA TGGGACCAAA TGCAGTTCTG GGAAGATGCA 
TTCTTAGATG CTGTAATGTT GGAAAGAGAA GGAATGGGTA TGGACCAGGG TCCTCAGGAA 
ATGATCGACA GGTACCTCTC CCTAGGGGAA CACGACCGGA AGCGCCTGGA GGATGATGAA 
GACCGCTTGC TGGCCACACT TTTACACAAC CTCATATCCT ATATGCTGCT GATGAAGGTG 
AACAAGAATG ACATCAGGAA GAAAGTACGG CGCCTAATGG GAAAGTCCCA CATTGGGCTG 
GTGTACAGCC AGCAAGTCAA TGAGGTGCTC GATCAGCTGA ACAGCCTGAA TGGACGTGAT 
CTCTCCATCC GGTCCAGCGG CAGCCGGCAC ATGAAGAAGC AGACTTTTGT TGTGCATGCA 
GGGACGGACA CAAATGGAGA CATCTTTTTC ATGGAAGTGT GTGATGACTG CGTGGTGTTG 
CGTAGTAACA TTGGGACGGT GTACGAGCGC TGGTGGTATG AGAAGCTCAT CAATATGACC 
TACTGTCCCA AGACCAAGGT CCTGTGCTTA TGGCGGAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACCAAGAA GTGTCGAGAG CTGTACTACT GTGTGAAGGA CAGCATGGAA 
CGTGCTGCTG CCCGACAGCA AAGCATCAAA CCTGGGCCTG AGCTAGGCGG TGAGTTCCCT 
GTGCAGGACA TGAAGACTGG AGAGGGTGGC TTGCTTCAAG TCACCCTGGA AGGGATCAAT 
CTCAAGTTCA TGCACAACCA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACA 
GTTCGAGGCG TCTTTGTCCT GGAAGAATTT GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC 
AAGTACAAGA CGCCAATGGC CCACGAGATC TGCTACTCTG TGTTGTGTCT CTTCTCGTAT 
GTGGCCGCAG TTCGTAGCAG TGAAGAAGAC CTTCGAACCC CACCTCGGCC TGTCTCTAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106069.1
CDS	337..5259
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250209.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250209.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGTTCC 
GGAGAGACCC TTGGAGCTGA CAGTGACCTG AGCAGCAATG CAGGTGACGG GCCAGGAGGC 
GAAGGCAGCG CCCACCTGGC AAGTTCCCGG GCCACTCTGT CCGACAGTGA AATCGAAACC 
AATTCTGCTA CAAGCGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAA GGAGAAGCCG 
GCTGGTAGTC CGATTCGCTC TTCCGAAGAC GTAAGCCAGC GAGTCTATCT CTATGAGGGA 
CTGCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGCTAG AGGATGCCGC TATGGAGACC 
TTTTCTCTGA GCAAAGAACG TTCTACCTTA TGGGACCAAA TGCAGTTCTG GGAAGATGCA 
TTCTTAGATG CTGTAATGTT GGAAAGAGAA GGAATGGGTA TGGACCAGGG TCCTCAGGAA 
ATGATCGACA GGTACCTCTC CCTAGGGGAA CACGACCGGA AGCGCCTGGA GGATGATGAA 
GACCGCTTGC TGGCCACACT TTTACACAAC CTCATATCCT ATATGCTGCT GATGAAGGTG 
AACAAGAATG ACATCAGGAA GAAAGTACGG CGCCTAATGG GAAAGTCCCA CATTGGGCTG 
GTGTACAGCC AGCAAGTCAA TGAGGTGCTC GATCAGCTGA ACAGCCTGAA TGGACGTGAT 
CTCTCCATCC GGTCCAGCGG CAGCCGGCAC ATGAAGAAGC AGACTTTTGT TGTGCATGCA 
GGGACGGACA CAAATGGAGA CATCTTTTTC ATGGAAGTGT GTGATGACTG CGTGGTGTTG 
CGTAGTAACA TTGGGACGGT GTACGAGCGC TGGTGGTATG AGAAGCTCAT CAATATGACC 
TACTGTCCCA AGACCAAGGT CCTGTGCTTA TGGCGGAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACCAAGAA GTGTCGAGAG CTGTACTACT GTGTGAAGGA CAGCATGGAA 
CGTGCTGCTG CCCGACAGCA AAGCATCAAA CCTGGGCCTG AGCTAGGCGG TGAGTTCCCT 
GTGCAGGACA TGAAGACTGG AGAGGGTGGC TTGCTTCAAG TCACCCTGGA AGGGATCAAT 
CTCAAGTTCA TGCACAACCA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACA 
GTTCGAGGCG TCTTTGTCCT GGAAGAATTT GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC 
AAGTACAAGA CGCCAATGGC CCACGAGATC TGCTACTCTG TGTTGTGTCT CTTCTCGTAT 
GTGGCCGCAG TTCGTAGCAG TGAAGAAGAC CTTCGAACCC CACCTCGGCC TGTCTCTAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250210.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106070.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu87825
Clone ID Related Accession (Same CDS sequence)	XM_030250210.1
Accession Version	XM_030250210.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGCTAGAG 
GATGCCGCTA TGGAGACCTT TTCTCTGAGC AAAGAACGTT CTACCTTATG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTAATGTTGG AAAGAGAAGG AATGGGTATG 
GACCAGGGTC CTCAGGAAAT GATCGACAGG TACCTCTCCC TAGGGGAACA CGACCGGAAG 
CGCCTGGAGG ATGATGAAGA CCGCTTGCTG GCCACACTTT TACACAACCT CATATCCTAT 
ATGCTGCTGA TGAAGGTGAA CAAGAATGAC ATCAGGAAGA AAGTACGGCG CCTAATGGGA 
AAGTCCCACA TTGGGCTGGT GTACAGCCAG CAAGTCAATG AGGTGCTCGA TCAGCTGAAC 
AGCCTGAATG GACGTGATCT CTCCATCCGG TCCAGCGGCA GCCGGCACAT GAAGAAGCAG 
ACTTTTGTTG TGCATGCAGG GACGGACACA AATGGAGACA TCTTTTTCAT GGAAGTGTGT 
GATGACTGCG TGGTGTTGCG TAGTAACATT GGGACGGTGT ACGAGCGCTG GTGGTATGAG 
AAGCTCATCA ATATGACCTA CTGTCCCAAG ACCAAGGTCC TGTGCTTATG GCGGAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACCAAGAAGT GTCGAGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAACG TGCTGCTGCC CGACAGCAAA GCATCAAACC TGGGCCTGAG 
CTAGGCGGTG AGTTCCCTGT GCAGGACATG AAGACTGGAG AGGGTGGCTT GCTTCAAGTC 
ACCCTGGAAG GGATCAATCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTT CGAGGCGTCT TTGTCCTGGA AGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACGC CAATGGCCCA CGAGATCTGC 
TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCCGCAGTTC GTAGCAGTGA AGAAGACCTT 
CGAACCCCAC CTCGGCCTGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106070.1
CDS	337..5226
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250210.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250210.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGCTAGAG 
GATGCCGCTA TGGAGACCTT TTCTCTGAGC AAAGAACGTT CTACCTTATG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTAATGTTGG AAAGAGAAGG AATGGGTATG 
GACCAGGGTC CTCAGGAAAT GATCGACAGG TACCTCTCCC TAGGGGAACA CGACCGGAAG 
CGCCTGGAGG ATGATGAAGA CCGCTTGCTG GCCACACTTT TACACAACCT CATATCCTAT 
ATGCTGCTGA TGAAGGTGAA CAAGAATGAC ATCAGGAAGA AAGTACGGCG CCTAATGGGA 
AAGTCCCACA TTGGGCTGGT GTACAGCCAG CAAGTCAATG AGGTGCTCGA TCAGCTGAAC 
AGCCTGAATG GACGTGATCT CTCCATCCGG TCCAGCGGCA GCCGGCACAT GAAGAAGCAG 
ACTTTTGTTG TGCATGCAGG GACGGACACA AATGGAGACA TCTTTTTCAT GGAAGTGTGT 
GATGACTGCG TGGTGTTGCG TAGTAACATT GGGACGGTGT ACGAGCGCTG GTGGTATGAG 
AAGCTCATCA ATATGACCTA CTGTCCCAAG ACCAAGGTCC TGTGCTTATG GCGGAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACCAAGAAGT GTCGAGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAACG TGCTGCTGCC CGACAGCAAA GCATCAAACC TGGGCCTGAG 
CTAGGCGGTG AGTTCCCTGT GCAGGACATG AAGACTGGAG AGGGTGGCTT GCTTCAAGTC 
ACCCTGGAAG GGATCAATCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTT CGAGGCGTCT TTGTCCTGGA AGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACGC CAATGGCCCA CGAGATCTGC 
TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCCGCAGTTC GTAGCAGTGA AGAAGACCTT 
CGAACCCCAC CTCGGCCTGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250214.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106074.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu87826
Clone ID Related Accession (Same CDS sequence)	XM_030250214.1
Accession Version	XM_030250214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGCTAGA GGATGCCGCT 
ATGGAGACCT TTTCTCTGAG CAAAGAACGT TCTACCTTAT GGGACCAAAT GCAGTTCTGG 
GAAGATGCAT TCTTAGATGC TGTAATGTTG GAAAGAGAAG GAATGGGTAT GGACCAGGGT 
CCTCAGGAAA TGATCGACAG GTACCTCTCC CTAGGGGAAC ACGACCGGAA GCGCCTGGAG 
GATGATGAAG ACCGCTTGCT GGCCACACTT TTACACAACC TCATATCCTA TATGCTGCTG 
ATGAAGGTGA ACAAGAATGA CATCAGGAAG AAAGTACGGC GCCTAATGGG AAAGTCCCAC 
ATTGGGCTGG TGTACAGCCA GCAAGTCAAT GAGGTGCTCG ATCAGCTGAA CAGCCTGAAT 
GGACGTGATC TCTCCATCCG GTCCAGCGGC AGCCGGCACA TGAAGAAGCA GACTTTTGTT 
GTGCATGCAG GGACGGACAC AAATGGAGAC ATCTTTTTCA TGGAAGTGTG TGATGACTGC 
GTGGTGTTGC GTAGTAACAT TGGGACGGTG TACGAGCGCT GGTGGTATGA GAAGCTCATC 
AATATGACCT ACTGTCCCAA GACCAAGGTC CTGTGCTTAT GGCGGAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACCAAGAAG TGTCGAGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAAC GTGCTGCTGC CCGACAGCAA AGCATCAAAC CTGGGCCTGA GCTAGGCGGT 
GAGTTCCCTG TGCAGGACAT GAAGACTGGA GAGGGTGGCT TGCTTCAAGT CACCCTGGAA 
GGGATCAATC TCAAGTTCAT GCACAACCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AAGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCCC ACGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCCGCAGTT CGTAGCAGTG AAGAAGACCT TCGAACCCCA 
CCTCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106074.1
CDS	337..5217
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250214.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250214.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGCTAGA GGATGCCGCT 
ATGGAGACCT TTTCTCTGAG CAAAGAACGT TCTACCTTAT GGGACCAAAT GCAGTTCTGG 
GAAGATGCAT TCTTAGATGC TGTAATGTTG GAAAGAGAAG GAATGGGTAT GGACCAGGGT 
CCTCAGGAAA TGATCGACAG GTACCTCTCC CTAGGGGAAC ACGACCGGAA GCGCCTGGAG 
GATGATGAAG ACCGCTTGCT GGCCACACTT TTACACAACC TCATATCCTA TATGCTGCTG 
ATGAAGGTGA ACAAGAATGA CATCAGGAAG AAAGTACGGC GCCTAATGGG AAAGTCCCAC 
ATTGGGCTGG TGTACAGCCA GCAAGTCAAT GAGGTGCTCG ATCAGCTGAA CAGCCTGAAT 
GGACGTGATC TCTCCATCCG GTCCAGCGGC AGCCGGCACA TGAAGAAGCA GACTTTTGTT 
GTGCATGCAG GGACGGACAC AAATGGAGAC ATCTTTTTCA TGGAAGTGTG TGATGACTGC 
GTGGTGTTGC GTAGTAACAT TGGGACGGTG TACGAGCGCT GGTGGTATGA GAAGCTCATC 
AATATGACCT ACTGTCCCAA GACCAAGGTC CTGTGCTTAT GGCGGAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACCAAGAAG TGTCGAGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAAC GTGCTGCTGC CCGACAGCAA AGCATCAAAC CTGGGCCTGA GCTAGGCGGT 
GAGTTCCCTG TGCAGGACAT GAAGACTGGA GAGGGTGGCT TGCTTCAAGT CACCCTGGAA 
GGGATCAATC TCAAGTTCAT GCACAACCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AAGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCCC ACGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCCGCAGTT CGTAGCAGTG AAGAAGACCT TCGAACCCCA 
CCTCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250216.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106076.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X14, mRNA.

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CloneID	OMu87827
Clone ID Related Accession (Same CDS sequence)	XM_030250216.1
Accession Version	XM_030250216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG CAAAGAACGT TCTACCTTAT GGGACCAAAT GCAGTTCTGG 
GAAGATGCAT TCTTAGATGC TGTAATGTTG GAAAGAGAAG GAATGGGTAT GGACCAGGGT 
CCTCAGGAAA TGATCGACAG GTACCTCTCC CTAGGGGAAC ACGACCGGAA GCGCCTGGAG 
GATGATGAAG ACCGCTTGCT GGCCACACTT TTACACAACC TCATATCCTA TATGCTGCTG 
ATGAAGGTGA ACAAGAATGA CATCAGGAAG AAAGTACGGC GCCTAATGGG AAAGTCCCAC 
ATTGGGCTGG TGTACAGCCA GCAAGTCAAT GAGGTGCTCG ATCAGCTGAA CAGCCTGAAT 
GGACGTGATC TCTCCATCCG GTCCAGCGGC AGCCGGCACA TGAAGAAGCA GACTTTTGTT 
GTGCATGCAG GGACGGACAC AAATGGAGAC ATCTTTTTCA TGGAAGTGTG TGATGACTGC 
GTGGTGTTGC GTAGTAACAT TGGGACGGTG TACGAGCGCT GGTGGTATGA GAAGCTCATC 
AATATGACCT ACTGTCCCAA GACCAAGGTC CTGTGCTTAT GGCGGAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACCAAGAAG TGTCGAGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAAC GTGCTGCTGC CCGACAGCAA AGCATCAAAC CTGGGCCTGA GCTAGGCGGT 
GAGTTCCCTG TGCAGGACAT GAAGACTGGA GAGGGTGGCT TGCTTCAAGT CACCCTGGAA 
GGGATCAATC TCAAGTTCAT GCACAACCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AAGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCCC ACGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCCGCAGTT CGTAGCAGTG AAGAAGACCT TCGAACCCCA 
CCTCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106076.1
CDS	337..5217
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250216.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250216.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG CAAAGAACGT TCTACCTTAT GGGACCAAAT GCAGTTCTGG 
GAAGATGCAT TCTTAGATGC TGTAATGTTG GAAAGAGAAG GAATGGGTAT GGACCAGGGT 
CCTCAGGAAA TGATCGACAG GTACCTCTCC CTAGGGGAAC ACGACCGGAA GCGCCTGGAG 
GATGATGAAG ACCGCTTGCT GGCCACACTT TTACACAACC TCATATCCTA TATGCTGCTG 
ATGAAGGTGA ACAAGAATGA CATCAGGAAG AAAGTACGGC GCCTAATGGG AAAGTCCCAC 
ATTGGGCTGG TGTACAGCCA GCAAGTCAAT GAGGTGCTCG ATCAGCTGAA CAGCCTGAAT 
GGACGTGATC TCTCCATCCG GTCCAGCGGC AGCCGGCACA TGAAGAAGCA GACTTTTGTT 
GTGCATGCAG GGACGGACAC AAATGGAGAC ATCTTTTTCA TGGAAGTGTG TGATGACTGC 
GTGGTGTTGC GTAGTAACAT TGGGACGGTG TACGAGCGCT GGTGGTATGA GAAGCTCATC 
AATATGACCT ACTGTCCCAA GACCAAGGTC CTGTGCTTAT GGCGGAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACCAAGAAG TGTCGAGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAAC GTGCTGCTGC CCGACAGCAA AGCATCAAAC CTGGGCCTGA GCTAGGCGGT 
GAGTTCCCTG TGCAGGACAT GAAGACTGGA GAGGGTGGCT TGCTTCAAGT CACCCTGGAA 
GGGATCAATC TCAAGTTCAT GCACAACCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AAGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCCC ACGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCCGCAGTT CGTAGCAGTG AAGAAGACCT TCGAACCCCA 
CCTCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250217.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106077.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X15, mRNA.

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CloneID	OMu87828
Clone ID Related Accession (Same CDS sequence)	XM_030250217.1
Accession Version	XM_030250217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGAGTAATA GTTCCGGAGA GACCCTTGGA GCTGACAGTG ACCTGAGCAG CAATGCAGGT 
GACGGGCCAG GAGGCGAAGG CAGCGCCCAC CTGGCAAGTT CCCGGGCCAC TCTGTCCGAC 
AGTGAAATCG AAACCAATTC TGCTACAAGC GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAAGGAGA AGCCGGCTGG TAGTCCGATT CGCTCTTCCG AAGACGTAAG CCAGCGAGTC 
TATCTCTATG AGGGACTGCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GCTAGAGGAT 
GCCGCTATGG AGACCTTTTC TCTGAGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAAG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCCCACG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC CGCAGTTCGT AGCAGTGAAG AAGACCTTCG AACCCCACCT 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106077.1
CDS	337..5214
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250217.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250217.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGAGTAATA GTTCCGGAGA GACCCTTGGA GCTGACAGTG ACCTGAGCAG CAATGCAGGT 
GACGGGCCAG GAGGCGAAGG CAGCGCCCAC CTGGCAAGTT CCCGGGCCAC TCTGTCCGAC 
AGTGAAATCG AAACCAATTC TGCTACAAGC GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAAGGAGA AGCCGGCTGG TAGTCCGATT CGCTCTTCCG AAGACGTAAG CCAGCGAGTC 
TATCTCTATG AGGGACTGCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GCTAGAGGAT 
GCCGCTATGG AGACCTTTTC TCTGAGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAAG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCCCACG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC CGCAGTTCGT AGCAGTGAAG AAGACCTTCG AACCCCACCT 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250218.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106078.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X16, mRNA.

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CloneID	OMu87829
Clone ID Related Accession (Same CDS sequence)	XM_030250218.1
Accession Version	XM_030250218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTGAGTAAT 
AGTTCCGGAG AGACCCTTGG AGCTGACAGT GACCTGAGCA GCAATGCAGG TGACGGGCCA 
GGAGGCGAAG GCAGCGCCCA CCTGGCAAGT TCCCGGGCCA CTCTGTCCGA CAGTGAAATC 
GAAACCAATT CTGCTACAAG CGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAAGGAG 
AAGCCGGCTG GTAGTCCGAT TCGCTCTTCC GAAGACGTAA GCCAGCGAGT CTATCTCTAT 
GAGGGACTGC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGCTAGAGGA TGCCGCTATG 
GAGACCTTTT CTCTGAGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAAG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCCCACG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC CGCAGTTCGT AGCAGTGAAG AAGACCTTCG AACCCCACCT 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106078.1
CDS	337..5214
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250218.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250218.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTGAGTAAT 
AGTTCCGGAG AGACCCTTGG AGCTGACAGT GACCTGAGCA GCAATGCAGG TGACGGGCCA 
GGAGGCGAAG GCAGCGCCCA CCTGGCAAGT TCCCGGGCCA CTCTGTCCGA CAGTGAAATC 
GAAACCAATT CTGCTACAAG CGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAAGGAG 
AAGCCGGCTG GTAGTCCGAT TCGCTCTTCC GAAGACGTAA GCCAGCGAGT CTATCTCTAT 
GAGGGACTGC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGCTAGAGGA TGCCGCTATG 
GAGACCTTTT CTCTGAGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAAG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCCCACG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC CGCAGTTCGT AGCAGTGAAG AAGACCTTCG AACCCCACCT 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250220.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106080.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X18, mRNA.

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CloneID	OMu87830
Clone ID Related Accession (Same CDS sequence)	XM_030250220.1
Accession Version	XM_030250220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG CAAAGAACGT TCTACCTTAT GGGACCAAAT GCAGTTCTGG 
GAAGATGCAT TCTTAGATGC TGTAATGTTG GAAAGAGAAG GAATGGGTAT GGACCAGGGT 
CCTCAGGAAA TGATCGACAG GTACCTCTCC CTAGGGGAAC ACGACCGGAA GCGCCTGGAG 
GATGATGAAG ACCGCTTGCT GGCCACACTT TTACACAACC TCATATCCTA TATGCTGCTG 
ATGAAGGTGA ACAAGAATGA CATCAGGAAG AAAGTACGGC GCCTAATGGG AAAGTCCCAC 
ATTGGGCTGG TGTACAGCCA GCAAGTCAAT GAGGTGCTCG ATCAGCTGAA CAGCCTGAAT 
GGACGTGATC TCTCCATCCG GTCCAGCGGC AGCCGGCACA TGAAGAAGCA GACTTTTGTT 
GTGCATGCAG GGACGGACAC AAATGGAGAC ATCTTTTTCA TGGAAGTGTG TGATGACTGC 
GTGGTGTTGC GTAGTAACAT TGGGACGGTG TACGAGCGCT GGTGGTATGA GAAGCTCATC 
AATATGACCT ACTGTCCCAA GACCAAGGTC CTGTGCTTAT GGCGGAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACCAAGAAG TGTCGAGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAAC GTGCTGCTGC CCGACAGCAA AGCATCAAAC CTGGGCCTGA GCTAGGCGGT 
GAGTTCCCTG TGCAGGACAT GAAGACTGGA GAGGGTGGCT TGCTTCAAGT CACCCTGGAA 
GGGATCAATC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TTCGAGGCGT CTTTGTCCTG GAAGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCC CACGAGATCT GCTACTCTGT GTTGTGTCTC 
TTCTCGTATG TGGCCGCAGT TCGTAGCAGT GAAGAAGACC TTCGAACCCC ACCTCGGCCT 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106080.1
CDS	337..5208
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250220.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250220.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG CAAAGAACGT TCTACCTTAT GGGACCAAAT GCAGTTCTGG 
GAAGATGCAT TCTTAGATGC TGTAATGTTG GAAAGAGAAG GAATGGGTAT GGACCAGGGT 
CCTCAGGAAA TGATCGACAG GTACCTCTCC CTAGGGGAAC ACGACCGGAA GCGCCTGGAG 
GATGATGAAG ACCGCTTGCT GGCCACACTT TTACACAACC TCATATCCTA TATGCTGCTG 
ATGAAGGTGA ACAAGAATGA CATCAGGAAG AAAGTACGGC GCCTAATGGG AAAGTCCCAC 
ATTGGGCTGG TGTACAGCCA GCAAGTCAAT GAGGTGCTCG ATCAGCTGAA CAGCCTGAAT 
GGACGTGATC TCTCCATCCG GTCCAGCGGC AGCCGGCACA TGAAGAAGCA GACTTTTGTT 
GTGCATGCAG GGACGGACAC AAATGGAGAC ATCTTTTTCA TGGAAGTGTG TGATGACTGC 
GTGGTGTTGC GTAGTAACAT TGGGACGGTG TACGAGCGCT GGTGGTATGA GAAGCTCATC 
AATATGACCT ACTGTCCCAA GACCAAGGTC CTGTGCTTAT GGCGGAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACCAAGAAG TGTCGAGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAAC GTGCTGCTGC CCGACAGCAA AGCATCAAAC CTGGGCCTGA GCTAGGCGGT 
GAGTTCCCTG TGCAGGACAT GAAGACTGGA GAGGGTGGCT TGCTTCAAGT CACCCTGGAA 
GGGATCAATC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TTCGAGGCGT CTTTGTCCTG GAAGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCC CACGAGATCT GCTACTCTGT GTTGTGTCTC 
TTCTCGTATG TGGCCGCAGT TCGTAGCAGT GAAGAAGACC TTCGAACCCC ACCTCGGCCT 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250221.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106081.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OMu87831
Clone ID Related Accession (Same CDS sequence)	XM_030250221.1
Accession Version	XM_030250221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGTTCC 
GGAGAGACCC TTGGAGCTGA CAGTGACCTG AGCAGCAATG CAGGTGACGG GCCAGGAGGC 
GAAGGCAGCG CCCACCTGGC AAGTTCCCGG GCCACTCTGT CCGACAGTGA AATCGAAACC 
AATTCTGCTA CAAGCGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAA GGAGAAGCCG 
GCTGGTAGTC CGATTCGCTC TTCCGAAGAC GTAAGCCAGC GAGTCTATCT CTATGAGGGA 
CTGCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGCTAG AGGATGCCGC TATGGAGACC 
TTTTCTCTGA GCAAAGAACG TTCTACCTTA TGGGACCAAA TGCAGTTCTG GGAAGATGCA 
TTCTTAGATG CTGTAATGTT GGAAAGAGAA GGAATGGGTA TGGACCAGGG TCCTCAGGAA 
ATGATCGACA GGTACCTCTC CCTAGGGGAA CACGACCGGA AGCGCCTGGA GGATGATGAA 
GACCGCTTGC TGGCCACACT TTTACACAAC CTCATATCCT ATATGCTGCT GATGAAGGTG 
AACAAGAATG ACATCAGGAA GAAAGTACGG CGCCTAATGG GAAAGTCCCA CATTGGGCTG 
GTGTACAGCC AGCAAGTCAA TGAGGTGCTC GATCAGCTGA ACAGCCTGAA TGGACGTGAT 
CTCTCCATCC GGTCCAGCGG CAGCCGGCAC ATGAAGAAGC AGACTTTTGT TGTGCATGCA 
GGGACGGACA CAAATGGAGA CATCTTTTTC ATGGAAGTGT GTGATGACTG CGTGGTGTTG 
CGTAGTAACA TTGGGACGGT GTACGAGCGC TGGTGGTATG AGAAGCTCAT CAATATGACC 
TACTGTCCCA AGACCAAGGT CCTGTGCTTA TGGCGGAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACCAAGAA GTGTCGAGAG CTGTACTACT GTGTGAAGGA CAGCATGGAA 
CGTGCTGCTG CCCGACAGCA AAGCATCAAA CCTGGGCCTG AGCTAGGCGG TGAGTTCCCT 
GTGCAGGACA TGAAGACTGG AGAGGGTGGC TTGCTTCAAG TCACCCTGGA AGGGATCAAT 
CTCAAGTTCA TGCACAACCA GGAGCGGAAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TTCGAGGCGT CTTTGTCCTG GAAGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCC CACGAGATCT GCTACTCTGT GTTGTGTCTC 
TTCTCGTATG TGGCCGCAGT TCGTAGCAGT GAAGAAGACC TTCGAACCCC ACCTCGGCCT 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106081.1
CDS	337..5208
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250221.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250221.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGTTCC 
GGAGAGACCC TTGGAGCTGA CAGTGACCTG AGCAGCAATG CAGGTGACGG GCCAGGAGGC 
GAAGGCAGCG CCCACCTGGC AAGTTCCCGG GCCACTCTGT CCGACAGTGA AATCGAAACC 
AATTCTGCTA CAAGCGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAA GGAGAAGCCG 
GCTGGTAGTC CGATTCGCTC TTCCGAAGAC GTAAGCCAGC GAGTCTATCT CTATGAGGGA 
CTGCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGCTAG AGGATGCCGC TATGGAGACC 
TTTTCTCTGA GCAAAGAACG TTCTACCTTA TGGGACCAAA TGCAGTTCTG GGAAGATGCA 
TTCTTAGATG CTGTAATGTT GGAAAGAGAA GGAATGGGTA TGGACCAGGG TCCTCAGGAA 
ATGATCGACA GGTACCTCTC CCTAGGGGAA CACGACCGGA AGCGCCTGGA GGATGATGAA 
GACCGCTTGC TGGCCACACT TTTACACAAC CTCATATCCT ATATGCTGCT GATGAAGGTG 
AACAAGAATG ACATCAGGAA GAAAGTACGG CGCCTAATGG GAAAGTCCCA CATTGGGCTG 
GTGTACAGCC AGCAAGTCAA TGAGGTGCTC GATCAGCTGA ACAGCCTGAA TGGACGTGAT 
CTCTCCATCC GGTCCAGCGG CAGCCGGCAC ATGAAGAAGC AGACTTTTGT TGTGCATGCA 
GGGACGGACA CAAATGGAGA CATCTTTTTC ATGGAAGTGT GTGATGACTG CGTGGTGTTG 
CGTAGTAACA TTGGGACGGT GTACGAGCGC TGGTGGTATG AGAAGCTCAT CAATATGACC 
TACTGTCCCA AGACCAAGGT CCTGTGCTTA TGGCGGAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACCAAGAA GTGTCGAGAG CTGTACTACT GTGTGAAGGA CAGCATGGAA 
CGTGCTGCTG CCCGACAGCA AAGCATCAAA CCTGGGCCTG AGCTAGGCGG TGAGTTCCCT 
GTGCAGGACA TGAAGACTGG AGAGGGTGGC TTGCTTCAAG TCACCCTGGA AGGGATCAAT 
CTCAAGTTCA TGCACAACCA GGAGCGGAAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TTCGAGGCGT CTTTGTCCTG GAAGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCAATGGCC CACGAGATCT GCTACTCTGT GTTGTGTCTC 
TTCTCGTATG TGGCCGCAGT TCGTAGCAGT GAAGAAGACC TTCGAACCCC ACCTCGGCCT 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250222.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106082.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X20, mRNA.

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CloneID	OMu87832
Clone ID Related Accession (Same CDS sequence)	XM_030250222.1
Accession Version	XM_030250222.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGTTCC 
GGAGAGACCC TTGGAGCTGA CAGTGACCTG AGCAGCAATG CAGGTGACGG GCCAGGAGGC 
GAAGGCAGCG CCCACCTGGC AAGTTCCCGG GCCACTCTGT CCGACAGTGA AATCGAAACC 
AATTCTGCTA CAAGCGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAA GGAGAAGCCG 
GCTGGTAGTC CGATTCGCTC TTCCGAAGAC GTAAGCCAGC GAGTCTATCT CTATGAGGGA 
CTGCTAGGCA AAGAACGTTC TACCTTATGG GACCAAATGC AGTTCTGGGA AGATGCATTC 
TTAGATGCTG TAATGTTGGA AAGAGAAGGA ATGGGTATGG ACCAGGGTCC TCAGGAAATG 
ATCGACAGGT ACCTCTCCCT AGGGGAACAC GACCGGAAGC GCCTGGAGGA TGATGAAGAC 
CGCTTGCTGG CCACACTTTT ACACAACCTC ATATCCTATA TGCTGCTGAT GAAGGTGAAC 
AAGAATGACA TCAGGAAGAA AGTACGGCGC CTAATGGGAA AGTCCCACAT TGGGCTGGTG 
TACAGCCAGC AAGTCAATGA GGTGCTCGAT CAGCTGAACA GCCTGAATGG ACGTGATCTC 
TCCATCCGGT CCAGCGGCAG CCGGCACATG AAGAAGCAGA CTTTTGTTGT GCATGCAGGG 
ACGGACACAA ATGGAGACAT CTTTTTCATG GAAGTGTGTG ATGACTGCGT GGTGTTGCGT 
AGTAACATTG GGACGGTGTA CGAGCGCTGG TGGTATGAGA AGCTCATCAA TATGACCTAC 
TGTCCCAAGA CCAAGGTCCT GTGCTTATGG CGGAGAAATG GCTCTGAGAC CCAGCTCAAC 
AAGTTCTATA CCAAGAAGTG TCGAGAGCTG TACTACTGTG TGAAGGACAG CATGGAACGT 
GCTGCTGCCC GACAGCAAAG CATCAAACCT GGGCCTGAGC TAGGCGGTGA GTTCCCTGTG 
CAGGACATGA AGACTGGAGA GGGTGGCTTG CTTCAAGTCA CCCTGGAAGG GATCAATCTC 
AAGTTCATGC ACAACCAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTT 
CGAGGCGTCT TTGTCCTGGA AGAATTTGTT CCTGAAATTA AAGAAGTGGT GAGCCACAAG 
TACAAGACGC CAATGGCCCA CGAGATCTGC TACTCTGTGT TGTGTCTCTT CTCGTATGTG 
GCCGCAGTTC GTAGCAGTGA AGAAGACCTT CGAACCCCAC CTCGGCCTGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106082.1
CDS	337..5196
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250222.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250222.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGTTCC 
GGAGAGACCC TTGGAGCTGA CAGTGACCTG AGCAGCAATG CAGGTGACGG GCCAGGAGGC 
GAAGGCAGCG CCCACCTGGC AAGTTCCCGG GCCACTCTGT CCGACAGTGA AATCGAAACC 
AATTCTGCTA CAAGCGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAA GGAGAAGCCG 
GCTGGTAGTC CGATTCGCTC TTCCGAAGAC GTAAGCCAGC GAGTCTATCT CTATGAGGGA 
CTGCTAGGCA AAGAACGTTC TACCTTATGG GACCAAATGC AGTTCTGGGA AGATGCATTC 
TTAGATGCTG TAATGTTGGA AAGAGAAGGA ATGGGTATGG ACCAGGGTCC TCAGGAAATG 
ATCGACAGGT ACCTCTCCCT AGGGGAACAC GACCGGAAGC GCCTGGAGGA TGATGAAGAC 
CGCTTGCTGG CCACACTTTT ACACAACCTC ATATCCTATA TGCTGCTGAT GAAGGTGAAC 
AAGAATGACA TCAGGAAGAA AGTACGGCGC CTAATGGGAA AGTCCCACAT TGGGCTGGTG 
TACAGCCAGC AAGTCAATGA GGTGCTCGAT CAGCTGAACA GCCTGAATGG ACGTGATCTC 
TCCATCCGGT CCAGCGGCAG CCGGCACATG AAGAAGCAGA CTTTTGTTGT GCATGCAGGG 
ACGGACACAA ATGGAGACAT CTTTTTCATG GAAGTGTGTG ATGACTGCGT GGTGTTGCGT 
AGTAACATTG GGACGGTGTA CGAGCGCTGG TGGTATGAGA AGCTCATCAA TATGACCTAC 
TGTCCCAAGA CCAAGGTCCT GTGCTTATGG CGGAGAAATG GCTCTGAGAC CCAGCTCAAC 
AAGTTCTATA CCAAGAAGTG TCGAGAGCTG TACTACTGTG TGAAGGACAG CATGGAACGT 
GCTGCTGCCC GACAGCAAAG CATCAAACCT GGGCCTGAGC TAGGCGGTGA GTTCCCTGTG 
CAGGACATGA AGACTGGAGA GGGTGGCTTG CTTCAAGTCA CCCTGGAAGG GATCAATCTC 
AAGTTCATGC ACAACCAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTT 
CGAGGCGTCT TTGTCCTGGA AGAATTTGTT CCTGAAATTA AAGAAGTGGT GAGCCACAAG 
TACAAGACGC CAATGGCCCA CGAGATCTGC TACTCTGTGT TGTGTCTCTT CTCGTATGTG 
GCCGCAGTTC GTAGCAGTGA AGAAGACCTT CGAACCCCAC CTCGGCCTGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250223.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106083.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X21, mRNA.

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CloneID	OMu87833
Clone ID Related Accession (Same CDS sequence)	XM_030250223.1
Accession Version	XM_030250223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGCTAGAG 
GATGCCGCTA TGGAGACCTT TTCTCTGAGC AAAGAACGTT CTACCTTATG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTAATGTTGG AAAGAGAAGG AATGGGTATG 
GACCAGGGTC CTCAGGAAAT GATCGACAGG TACCTCTCCC TAGGGGAACA CGACCGGAAG 
CGCCTGGAGG ATGATGAAGA CCGCTTGCTG GCCACACTTT TACACAACCT CATATCCTAT 
ATGCTGCTGA TGAAGGTGAA CAAGAATGAC ATCAGGAAGA AAGTACGGCG CCTAATGGGA 
AAGTCCCACA TTGGGCTGGT GTACAGCCAG CAAGTCAATG AGGTGCTCGA TCAGCTGAAC 
AGCCTGAATG GACGTGATCT CTCCATCCGG TCCAGCGGCA GCCGGCACAT GAAGAAGCAG 
ACTTTTGTTG TGCATGCAGG GACGGACACA AATGGAGACA TCTTTTTCAT GGAAGTGTGT 
GATGACTGCG TGGTGTTGCG TAGTAACATT GGGACGGTGT ACGAGCGCTG GTGGTATGAG 
AAGCTCATCA ATATGACCTA CTGTCCCAAG ACCAAGGTCC TGTGCTTATG GCGGAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACCAAGAAGT GTCGAGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAACG TGCTGCTGCC CGACAGCAAA GCATCAAACC TGGGCCTGAG 
CTAGGCGGTG AGTTCCCTGT GCAGGACATG AAGACTGGAG AGGGTGGCTT GCTTCAAGTC 
ACCCTGGAAG GGATCAATCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTT CGAGGCGTCT TTGTCCTGGA AGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACGC CAATGGCCCA CGAGATCTGC 
TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCCGCAGTTC GTAGCAGTGA AGAAGACCTT 
CGAACCCCAC CTCGGCCTGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106083.1
CDS	337..5166
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250223.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250223.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGCTAGAG 
GATGCCGCTA TGGAGACCTT TTCTCTGAGC AAAGAACGTT CTACCTTATG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTAATGTTGG AAAGAGAAGG AATGGGTATG 
GACCAGGGTC CTCAGGAAAT GATCGACAGG TACCTCTCCC TAGGGGAACA CGACCGGAAG 
CGCCTGGAGG ATGATGAAGA CCGCTTGCTG GCCACACTTT TACACAACCT CATATCCTAT 
ATGCTGCTGA TGAAGGTGAA CAAGAATGAC ATCAGGAAGA AAGTACGGCG CCTAATGGGA 
AAGTCCCACA TTGGGCTGGT GTACAGCCAG CAAGTCAATG AGGTGCTCGA TCAGCTGAAC 
AGCCTGAATG GACGTGATCT CTCCATCCGG TCCAGCGGCA GCCGGCACAT GAAGAAGCAG 
ACTTTTGTTG TGCATGCAGG GACGGACACA AATGGAGACA TCTTTTTCAT GGAAGTGTGT 
GATGACTGCG TGGTGTTGCG TAGTAACATT GGGACGGTGT ACGAGCGCTG GTGGTATGAG 
AAGCTCATCA ATATGACCTA CTGTCCCAAG ACCAAGGTCC TGTGCTTATG GCGGAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACCAAGAAGT GTCGAGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAACG TGCTGCTGCC CGACAGCAAA GCATCAAACC TGGGCCTGAG 
CTAGGCGGTG AGTTCCCTGT GCAGGACATG AAGACTGGAG AGGGTGGCTT GCTTCAAGTC 
ACCCTGGAAG GGATCAATCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTT CGAGGCGTCT TTGTCCTGGA AGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACGC CAATGGCCCA CGAGATCTGC 
TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCCGCAGTTC GTAGCAGTGA AGAAGACCTT 
CGAACCCCAC CTCGGCCTGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250224.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106084.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X22, mRNA.

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CloneID	OMu87834
Clone ID Related Accession (Same CDS sequence)	XM_030250224.1
Accession Version	XM_030250224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAAGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACGCCAA TGGCCCACGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCC GCAGTTCGTA GCAGTGAAGA AGACCTTCGA 
ACCCCACCTC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106084.1
CDS	337..5163
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250224.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250224.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAAGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACGCCAA TGGCCCACGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCC GCAGTTCGTA GCAGTGAAGA AGACCTTCGA 
ACCCCACCTC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250225.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106085.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X23, mRNA.

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CloneID	OMu87835
Clone ID Related Accession (Same CDS sequence)	XM_030250225.1
Accession Version	XM_030250225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGCTAGAG 
GATGCCGCTA TGGAGACCTT TTCTCTGAGC AAAGAACGTT CTACCTTATG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTAATGTTGG AAAGAGAAGG AATGGGTATG 
GACCAGGGTC CTCAGGAAAT GATCGACAGG TACCTCTCCC TAGGGGAACA CGACCGGAAG 
CGCCTGGAGG ATGATGAAGA CCGCTTGCTG GCCACACTTT TACACAACCT CATATCCTAT 
ATGCTGCTGA TGAAGGTGAA CAAGAATGAC ATCAGGAAGA AAGTACGGCG CCTAATGGGA 
AAGTCCCACA TTGGGCTGGT GTACAGCCAG CAAGTCAATG AGGTGCTCGA TCAGCTGAAC 
AGCCTGAATG GACGTGATCT CTCCATCCGG TCCAGCGGCA GCCGGCACAT GAAGAAGCAG 
ACTTTTGTTG TGCATGCAGG GACGGACACA AATGGAGACA TCTTTTTCAT GGAAGTGTGT 
GATGACTGCG TGGTGTTGCG TAGTAACATT GGGACGGTGT ACGAGCGCTG GTGGTATGAG 
AAGCTCATCA ATATGACCTA CTGTCCCAAG ACCAAGGTCC TGTGCTTATG GCGGAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACCAAGAAGT GTCGAGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAACG TGCTGCTGCC CGACAGCAAA GCATCAAACC TGGGCCTGAG 
CTAGGCGGTG AGTTCCCTGT GCAGGACATG AAGACTGGAG AGGGTGGCTT GCTTCAAGTC 
ACCCTGGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AAGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCCC ACGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCCGCAGTT CGTAGCAGTG AAGAAGACCT TCGAACCCCA 
CCTCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106085.1
CDS	337..5157
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250225.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250225.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGCTAGAG 
GATGCCGCTA TGGAGACCTT TTCTCTGAGC AAAGAACGTT CTACCTTATG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTAATGTTGG AAAGAGAAGG AATGGGTATG 
GACCAGGGTC CTCAGGAAAT GATCGACAGG TACCTCTCCC TAGGGGAACA CGACCGGAAG 
CGCCTGGAGG ATGATGAAGA CCGCTTGCTG GCCACACTTT TACACAACCT CATATCCTAT 
ATGCTGCTGA TGAAGGTGAA CAAGAATGAC ATCAGGAAGA AAGTACGGCG CCTAATGGGA 
AAGTCCCACA TTGGGCTGGT GTACAGCCAG CAAGTCAATG AGGTGCTCGA TCAGCTGAAC 
AGCCTGAATG GACGTGATCT CTCCATCCGG TCCAGCGGCA GCCGGCACAT GAAGAAGCAG 
ACTTTTGTTG TGCATGCAGG GACGGACACA AATGGAGACA TCTTTTTCAT GGAAGTGTGT 
GATGACTGCG TGGTGTTGCG TAGTAACATT GGGACGGTGT ACGAGCGCTG GTGGTATGAG 
AAGCTCATCA ATATGACCTA CTGTCCCAAG ACCAAGGTCC TGTGCTTATG GCGGAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACCAAGAAGT GTCGAGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAACG TGCTGCTGCC CGACAGCAAA GCATCAAACC TGGGCCTGAG 
CTAGGCGGTG AGTTCCCTGT GCAGGACATG AAGACTGGAG AGGGTGGCTT GCTTCAAGTC 
ACCCTGGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AAGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCAATGGCCC ACGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCCGCAGTT CGTAGCAGTG AAGAAGACCT TCGAACCCCA 
CCTCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250230.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106090.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OMu87836
Clone ID Related Accession (Same CDS sequence)	XM_030250230.1
Accession Version	XM_030250230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GTATTTGGGC TAAATACTCT AATGGAGATT GTTACTGAAG CCGGCCCTGG GAGTGGTGAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAGGCT TTGGAAAAAC AGAGGCCAGA GGGCATCAAG 
CCTGTCTTTG ACCTGGGTGA GACAGAGGAG AAAAAGTCCC AGATGAGCGC AGACAGTGGT 
GTGAGCCTGA CATCTGCCTC CCAGAGGACT GATCAAGACT CTGTCATCGG TGTGAGTCCA 
GCTGTTATGA TCCGAAGCTC AAGTCAGGAT TCTGAAGTTA GCACCGTGGT GAGTAATAGT 
TCCGGAGAGA CCCTTGGAGC TGACAGTGAC CTGAGCAGCA ATGCAGGTGA CGGGCCAGGA 
GGCGAAGGCA GCGCCCACCT GGCAAGTTCC CGGGCCACTC TGTCCGACAG TGAAATCGAA 
ACCAATTCTG CTACAAGCGC CATCTTTGGT AAAGCCCATA GCTTGAAGCC AAAGGAGAAG 
CCGGCTGGTA GTCCGATTCG CTCTTCCGAA GACGTAAGCC AGCGAGTCTA TCTCTATGAG 
GGACTGCTAG GAAGGGACAA AGGATCCATG TGGGACCAGC TAGAGGATGC CGCTATGGAG 
ACCTTTTCTC TGAGCAAAGA ACGTTCTACC TTATGGGACC AAATGCAGTT CTGGGAAGAT 
GCATTCTTAG ATGCTGTAAT GTTGGAAAGA GAAGGAATGG GTATGGACCA GGGTCCTCAG 
GAAATGATCG ACAGGTACCT CTCCCTAGGG GAACACGACC GGAAGCGCCT GGAGGATGAT 
GAAGACCGCT TGCTGGCCAC ACTTTTACAC AACCTCATAT CCTATATGCT GCTGATGAAG 
GTGAACAAGA ATGACATCAG GAAGAAAGTA CGGCGCCTAA TGGGAAAGTC CCACATTGGG 
CTGGTGTACA GCCAGCAAGT CAATGAGGTG CTCGATCAGC TGAACAGCCT GAATGGACGT 
GATCTCTCCA TCCGGTCCAG CGGCAGCCGG CACATGAAGA AGCAGACTTT TGTTGTGCAT 
GCAGGGACGG ACACAAATGG AGACATCTTT TTCATGGAAG TGTGTGATGA CTGCGTGGTG 
TTGCGTAGTA ACATTGGGAC GGTGTACGAG CGCTGGTGGT ATGAGAAGCT CATCAATATG 
ACCTACTGTC CCAAGACCAA GGTCCTGTGC TTATGGCGGA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACCAA GAAGTGTCGA GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAACGTGCTG CTGCCCGACA GCAAAGCATC AAACCTGGGC CTGAGCTAGG CGGTGAGTTC 
CCTGTGCAGG ACATGAAGAC TGGAGAGGGT GGCTTGCTTC AAGTCACCCT GGAAGGGATC 
AATCTCAAGT TCATGCACAA CCAGGAGCGG AAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106090.1
CDS	337..5151
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250230.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250230.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GTATTTGGGC TAAATACTCT AATGGAGATT GTTACTGAAG CCGGCCCTGG GAGTGGTGAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAGGCT TTGGAAAAAC AGAGGCCAGA GGGCATCAAG 
CCTGTCTTTG ACCTGGGTGA GACAGAGGAG AAAAAGTCCC AGATGAGCGC AGACAGTGGT 
GTGAGCCTGA CATCTGCCTC CCAGAGGACT GATCAAGACT CTGTCATCGG TGTGAGTCCA 
GCTGTTATGA TCCGAAGCTC AAGTCAGGAT TCTGAAGTTA GCACCGTGGT GAGTAATAGT 
TCCGGAGAGA CCCTTGGAGC TGACAGTGAC CTGAGCAGCA ATGCAGGTGA CGGGCCAGGA 
GGCGAAGGCA GCGCCCACCT GGCAAGTTCC CGGGCCACTC TGTCCGACAG TGAAATCGAA 
ACCAATTCTG CTACAAGCGC CATCTTTGGT AAAGCCCATA GCTTGAAGCC AAAGGAGAAG 
CCGGCTGGTA GTCCGATTCG CTCTTCCGAA GACGTAAGCC AGCGAGTCTA TCTCTATGAG 
GGACTGCTAG GAAGGGACAA AGGATCCATG TGGGACCAGC TAGAGGATGC CGCTATGGAG 
ACCTTTTCTC TGAGCAAAGA ACGTTCTACC TTATGGGACC AAATGCAGTT CTGGGAAGAT 
GCATTCTTAG ATGCTGTAAT GTTGGAAAGA GAAGGAATGG GTATGGACCA GGGTCCTCAG 
GAAATGATCG ACAGGTACCT CTCCCTAGGG GAACACGACC GGAAGCGCCT GGAGGATGAT 
GAAGACCGCT TGCTGGCCAC ACTTTTACAC AACCTCATAT CCTATATGCT GCTGATGAAG 
GTGAACAAGA ATGACATCAG GAAGAAAGTA CGGCGCCTAA TGGGAAAGTC CCACATTGGG 
CTGGTGTACA GCCAGCAAGT CAATGAGGTG CTCGATCAGC TGAACAGCCT GAATGGACGT 
GATCTCTCCA TCCGGTCCAG CGGCAGCCGG CACATGAAGA AGCAGACTTT TGTTGTGCAT 
GCAGGGACGG ACACAAATGG AGACATCTTT TTCATGGAAG TGTGTGATGA CTGCGTGGTG 
TTGCGTAGTA ACATTGGGAC GGTGTACGAG CGCTGGTGGT ATGAGAAGCT CATCAATATG 
ACCTACTGTC CCAAGACCAA GGTCCTGTGC TTATGGCGGA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACCAA GAAGTGTCGA GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAACGTGCTG CTGCCCGACA GCAAAGCATC AAACCTGGGC CTGAGCTAGG CGGTGAGTTC 
CCTGTGCAGG ACATGAAGAC TGGAGAGGGT GGCTTGCTTC AAGTCACCCT GGAAGGGATC 
AATCTCAAGT TCATGCACAA CCAGGAGCGG AAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250233.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106093.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OMu87837
Clone ID Related Accession (Same CDS sequence)	XM_030250233.1
Accession Version	XM_030250233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGAGTAATA GTTCCGGAGA GACCCTTGGA GCTGACAGTG ACCTGAGCAG CAATGCAGGT 
GACGGGCCAG GAGGCGAAGG CAGCGCCCAC CTGGCAAGTT CCCGGGCCAC TCTGTCCGAC 
AGTGAAATCG AAACCAATTC TGCTACAAGC GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAAGGAGA AGCCGGCTGG TAGTCCGATT CGCTCTTCCG AAGACGTAAG CCAGCGAGTC 
TATCTCTATG AGGGACTGCT AGGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106093.1
CDS	337..5151
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250233.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250233.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGAGTAATA GTTCCGGAGA GACCCTTGGA GCTGACAGTG ACCTGAGCAG CAATGCAGGT 
GACGGGCCAG GAGGCGAAGG CAGCGCCCAC CTGGCAAGTT CCCGGGCCAC TCTGTCCGAC 
AGTGAAATCG AAACCAATTC TGCTACAAGC GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAAGGAGA AGCCGGCTGG TAGTCCGATT CGCTCTTCCG AAGACGTAAG CCAGCGAGTC 
TATCTCTATG AGGGACTGCT AGGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250236.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106096.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X27, mRNA.

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CloneID	OMu87838
Clone ID Related Accession (Same CDS sequence)	XM_030250236.1
Accession Version	XM_030250236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTGAGTAAT 
AGTTCCGGAG AGACCCTTGG AGCTGACAGT GACCTGAGCA GCAATGCAGG TGACGGGCCA 
GGAGGCGAAG GCAGCGCCCA CCTGGCAAGT TCCCGGGCCA CTCTGTCCGA CAGTGAAATC 
GAAACCAATT CTGCTACAAG CGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAAGGAG 
AAGCCGGCTG GTAGTCCGAT TCGCTCTTCC GAAGACGTAA GCCAGCGAGT CTATCTCTAT 
GAGGGACTGC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGCTAGAGGA TGCCGCTATG 
GAGACCTTTT CTCTGAGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTTC GAGGCGTCTT TGTCCTGGAA GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC AATGGCCCAC GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CCGCAGTTCG TAGCAGTGAA GAAGACCTTC GAACCCCACC TCGGCCTGTC 
TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106096.1
CDS	337..5145
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250236.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250236.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTGAGTAAT 
AGTTCCGGAG AGACCCTTGG AGCTGACAGT GACCTGAGCA GCAATGCAGG TGACGGGCCA 
GGAGGCGAAG GCAGCGCCCA CCTGGCAAGT TCCCGGGCCA CTCTGTCCGA CAGTGAAATC 
GAAACCAATT CTGCTACAAG CGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAAGGAG 
AAGCCGGCTG GTAGTCCGAT TCGCTCTTCC GAAGACGTAA GCCAGCGAGT CTATCTCTAT 
GAGGGACTGC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGCTAGAGGA TGCCGCTATG 
GAGACCTTTT CTCTGAGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTTC GAGGCGTCTT TGTCCTGGAA GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC AATGGCCCAC GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CCGCAGTTCG TAGCAGTGAA GAAGACCTTC GAACCCCACC TCGGCCTGTC 
TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250239.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106099.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X30, mRNA.

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CloneID	OMu87839
Clone ID Related Accession (Same CDS sequence)	XM_030250239.1
Accession Version	XM_030250239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAGGCT TTGGAAAAAC AGAGGCCAGA GGGCATCAAG 
CCTGTCTTTG ACCTGGGTGA GACAGAGGAG AAAAAGTCCC AGATGAGCGC AGACAGTGGT 
GTGAGCCTGA CATCTGCCTC CCAGAGGACT GATCAAGACT CTGTCATCGG TGTGAGTCCA 
GCTGTTATGA TCCGAAGCTC AAGTCAGGAT TCTGAAGTTA GCACCGTGGT GAGTAATAGT 
TCCGGAGAGA CCCTTGGAGC TGACAGTGAC CTGAGCAGCA ATGCAGGTGA CGGGCCAGGA 
GGCGAAGGCA GCGCCCACCT GGCAAGTTCC CGGGCCACTC TGTCCGACAG TGAAATCGAA 
ACCAATTCTG CTACAAGCGC CATCTTTGGT AAAGCCCATA GCTTGAAGCC AAAGGAGAAG 
CCGGCTGGTA GTCCGATTCG CTCTTCCGAA GACGTAAGCC AGCGAGTCTA TCTCTATGAG 
GGACTGCTAG GAAGGGACAA AGGATCCATG TGGGACCAGC TAGAGGATGC CGCTATGGAG 
ACCTTTTCTC TGAGCAAAGA ACGTTCTACC TTATGGGACC AAATGCAGTT CTGGGAAGAT 
GCATTCTTAG ATGCTGTAAT GTTGGAAAGA GAAGGAATGG GTATGGACCA GGGTCCTCAG 
GAAATGATCG ACAGGTACCT CTCCCTAGGG GAACACGACC GGAAGCGCCT GGAGGATGAT 
GAAGACCGCT TGCTGGCCAC ACTTTTACAC AACCTCATAT CCTATATGCT GCTGATGAAG 
GTGAACAAGA ATGACATCAG GAAGAAAGTA CGGCGCCTAA TGGGAAAGTC CCACATTGGG 
CTGGTGTACA GCCAGCAAGT CAATGAGGTG CTCGATCAGC TGAACAGCCT GAATGGACGT 
GATCTCTCCA TCCGGTCCAG CGGCAGCCGG CACATGAAGA AGCAGACTTT TGTTGTGCAT 
GCAGGGACGG ACACAAATGG AGACATCTTT TTCATGGAAG TGTGTGATGA CTGCGTGGTG 
TTGCGTAGTA ACATTGGGAC GGTGTACGAG CGCTGGTGGT ATGAGAAGCT CATCAATATG 
ACCTACTGTC CCAAGACCAA GGTCCTGTGC TTATGGCGGA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACCAA GAAGTGTCGA GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAACGTGCTG CTGCCCGACA GCAAAGCATC AAACCTGGGC CTGAGCTAGG CGGTGAGTTC 
CCTGTGCAGG ACATGAAGAC TGGAGAGGGT GGCTTGCTTC AAGTCACCCT GGAAGGGATC 
AATCTCAAGT TCATGCACAA CCAGGAGCGG AAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106099.1
CDS	337..5091
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_030250239.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250239.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGA ATTGTGGAAC 
AAGCACCAGG AAGTGAAAAA GCAAAAGGCT TTGGAAAAAC AGAGGCCAGA GGGCATCAAG 
CCTGTCTTTG ACCTGGGTGA GACAGAGGAG AAAAAGTCCC AGATGAGCGC AGACAGTGGT 
GTGAGCCTGA CATCTGCCTC CCAGAGGACT GATCAAGACT CTGTCATCGG TGTGAGTCCA 
GCTGTTATGA TCCGAAGCTC AAGTCAGGAT TCTGAAGTTA GCACCGTGGT GAGTAATAGT 
TCCGGAGAGA CCCTTGGAGC TGACAGTGAC CTGAGCAGCA ATGCAGGTGA CGGGCCAGGA 
GGCGAAGGCA GCGCCCACCT GGCAAGTTCC CGGGCCACTC TGTCCGACAG TGAAATCGAA 
ACCAATTCTG CTACAAGCGC CATCTTTGGT AAAGCCCATA GCTTGAAGCC AAAGGAGAAG 
CCGGCTGGTA GTCCGATTCG CTCTTCCGAA GACGTAAGCC AGCGAGTCTA TCTCTATGAG 
GGACTGCTAG GAAGGGACAA AGGATCCATG TGGGACCAGC TAGAGGATGC CGCTATGGAG 
ACCTTTTCTC TGAGCAAAGA ACGTTCTACC TTATGGGACC AAATGCAGTT CTGGGAAGAT 
GCATTCTTAG ATGCTGTAAT GTTGGAAAGA GAAGGAATGG GTATGGACCA GGGTCCTCAG 
GAAATGATCG ACAGGTACCT CTCCCTAGGG GAACACGACC GGAAGCGCCT GGAGGATGAT 
GAAGACCGCT TGCTGGCCAC ACTTTTACAC AACCTCATAT CCTATATGCT GCTGATGAAG 
GTGAACAAGA ATGACATCAG GAAGAAAGTA CGGCGCCTAA TGGGAAAGTC CCACATTGGG 
CTGGTGTACA GCCAGCAAGT CAATGAGGTG CTCGATCAGC TGAACAGCCT GAATGGACGT 
GATCTCTCCA TCCGGTCCAG CGGCAGCCGG CACATGAAGA AGCAGACTTT TGTTGTGCAT 
GCAGGGACGG ACACAAATGG AGACATCTTT TTCATGGAAG TGTGTGATGA CTGCGTGGTG 
TTGCGTAGTA ACATTGGGAC GGTGTACGAG CGCTGGTGGT ATGAGAAGCT CATCAATATG 
ACCTACTGTC CCAAGACCAA GGTCCTGTGC TTATGGCGGA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACCAA GAAGTGTCGA GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAACGTGCTG CTGCCCGACA GCAAAGCATC AAACCTGGGC CTGAGCTAGG CGGTGAGTTC 
CCTGTGCAGG ACATGAAGAC TGGAGAGGGT GGCTTGCTTC AAGTCACCCT GGAAGGGATC 
AATCTCAAGT TCATGCACAA CCAGGAGCGG AAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250244.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106104.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X34, mRNA.

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CloneID	OMu87840
Clone ID Related Accession (Same CDS sequence)	XM_030250244.1
Accession Version	XM_030250244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGTTCC 
GGAGAGACCC TTGGAGCTGA CAGTGACCTG AGCAGCAATG CAGGTGACGG GCCAGGAGGC 
GAAGGCAGCG CCCACCTGGC AAGTTCCCGG GCCACTCTGT CCGACAGTGA AATCGAAACC 
AATTCTGCTA CAAGCGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAA GGAGAAGCCG 
GCTGGTAGTC CGATTCGCTC TTCCGAAGAC GTAAGCCAGC GAGTCTATCT CTATGAGGGA 
CTGCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGCTAG AGGATGCCGC TATGGAGACC 
TTTTCTCTGA GCAAAGAACG TTCTACCTTA TGGGACCAAA TGCAGTTCTG GGAAGATGCA 
TTCTTAGATG CTGTAATGTT GGAAAGAGAA GGAATGGGTA TGGACCAGGG TCCTCAGGAA 
ATGATCGACA GGTACCTCTC CCTAGGGGAA CACGACCGGA AGCGCCTGGA GGATGATGAA 
GACCGCTTGC TGGCCACACT TTTACACAAC CTCATATCCT ATATGCTGCT GATGAAGGTG 
AACAAGAATG ACATCAGGAA GAAAGTACGG CGCCTAATGG GAAAGTCCCA CATTGGGCTG 
GTGTACAGCC AGCAAGTCAA TGAGGTGCTC GATCAGCTGA ACAGCCTGAA TGGACGTGAT 
CTCTCCATCC GGTCCAGCGG CAGCCGGCAC ATGAAGAAGC AGACTTTTGT TGTGCATGCA 
GGGACGGACA CAAATGGAGA CATCTTTTTC ATGGAAGTGT GTGATGACTG CGTGGTGTTG 
CGTAGTAACA TTGGGACGGT GTACGAGCGC TGGTGGTATG AGAAGCTCAT CAATATGACC 
TACTGTCCCA AGACCAAGGT CCTGTGCTTA TGGCGGAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACCAAGAA GTGTCGAGAG CTGTACTACT GTGTGAAGGA CAGCATGGAA 
CGTGCTGCTG CCCGACAGCA AAGCATCAAA CCTGGGCCTG AGCTAGGCGG TGAGTTCCCT 
GTGCAGGACA TGAAGACTGG AGAGGGTGGC TTGCTTCAAG TCACCCTGGA AGGGATCAAT 
CTCAAGTTCA TGCACAACCA GTTCCTGAAA TTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106104.1
CDS	337..5061
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_030250244.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250244.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGTTCC 
GGAGAGACCC TTGGAGCTGA CAGTGACCTG AGCAGCAATG CAGGTGACGG GCCAGGAGGC 
GAAGGCAGCG CCCACCTGGC AAGTTCCCGG GCCACTCTGT CCGACAGTGA AATCGAAACC 
AATTCTGCTA CAAGCGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAA GGAGAAGCCG 
GCTGGTAGTC CGATTCGCTC TTCCGAAGAC GTAAGCCAGC GAGTCTATCT CTATGAGGGA 
CTGCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGCTAG AGGATGCCGC TATGGAGACC 
TTTTCTCTGA GCAAAGAACG TTCTACCTTA TGGGACCAAA TGCAGTTCTG GGAAGATGCA 
TTCTTAGATG CTGTAATGTT GGAAAGAGAA GGAATGGGTA TGGACCAGGG TCCTCAGGAA 
ATGATCGACA GGTACCTCTC CCTAGGGGAA CACGACCGGA AGCGCCTGGA GGATGATGAA 
GACCGCTTGC TGGCCACACT TTTACACAAC CTCATATCCT ATATGCTGCT GATGAAGGTG 
AACAAGAATG ACATCAGGAA GAAAGTACGG CGCCTAATGG GAAAGTCCCA CATTGGGCTG 
GTGTACAGCC AGCAAGTCAA TGAGGTGCTC GATCAGCTGA ACAGCCTGAA TGGACGTGAT 
CTCTCCATCC GGTCCAGCGG CAGCCGGCAC ATGAAGAAGC AGACTTTTGT TGTGCATGCA 
GGGACGGACA CAAATGGAGA CATCTTTTTC ATGGAAGTGT GTGATGACTG CGTGGTGTTG 
CGTAGTAACA TTGGGACGGT GTACGAGCGC TGGTGGTATG AGAAGCTCAT CAATATGACC 
TACTGTCCCA AGACCAAGGT CCTGTGCTTA TGGCGGAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACCAAGAA GTGTCGAGAG CTGTACTACT GTGTGAAGGA CAGCATGGAA 
CGTGCTGCTG CCCGACAGCA AAGCATCAAA CCTGGGCCTG AGCTAGGCGG TGAGTTCCCT 
GTGCAGGACA TGAAGACTGG AGAGGGTGGC TTGCTTCAAG TCACCCTGGA AGGGATCAAT 
CTCAAGTTCA TGCACAACCA GTTCCTGAAA TTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250246.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106106.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X36, mRNA.

Price & Availability↑

CloneID	OMu87841
Clone ID Related Accession (Same CDS sequence)	XM_030250246.1
Accession Version	XM_030250246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGTTCC 
GGAGAGACCC TTGGAGCTGA CAGTGACCTG AGCAGCAATG CAGGTGACGG GCCAGGAGGC 
GAAGGCAGCG CCCACCTGGC AAGTTCCCGG GCCACTCTGT CCGACAGTGA AATCGAAACC 
AATTCTGCTA CAAGCGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAA GGAGAAGCCG 
GCTGGTAGTC CGATTCGCTC TTCCGAAGAC GTAAGCCAGC GAGTCTATCT CTATGAGGGA 
CTGCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGCTAG AGGATGCCGC TATGGAGACC 
TTTTCTCTGA GCAAAGAACG TTCTACCTTA TGGGACCAAA TGCAGTTCTG GGAAGATGCA 
TTCTTAGATG CTGTAATGTT GGAAAGAGAA GGAATGGGTA TGGACCAGGG TCCTCAGGAA 
ATGATCGACA GGTACCTCTC CCTAGGGGAA CACGACCGGA AGCGCCTGGA GGATGATGAA 
GACCGCTTGC TGGCCACACT TTTACACAAC CTCATATCCT ATATGCTGCT GATGAAGGTG 
AACAAGAATG ACATCAGGAA GAAAGTACGG CGCCTAATGG GAAAGTCCCA CATTGGGCTG 
GTGTACAGCC AGCAAGTCAA TGAGGTGCTC GATCAGCTGA ACAGCCTGAA TGGACGTGAT 
CTCTCCATCC GGTCCAGCGG CAGCCGGCAC ATGAAGAAGC AGACTTTTGT TGTGCATGCA 
GGGACGGACA CAAATGGAGA CATCTTTTTC ATGGAAGTGT GTGATGACTG CGTGGTGTTG 
CGTAGTAACA TTGGGACGGT GTACGAGCGC TGGTGGTATG AGAAGCTCAT CAATATGACC 
TACTGTCCCA AGACCAAGGT CCTGTGCTTA TGGCGGAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACCAAGAA GTGTCGAGAG CTGTACTACT GTGTGAAGGA CAGCATGGAA 
CGTGCTGCTG CCCGACAGCA AAGCATCAAA CCTGGGCCTG AGCTAGGCGG TGAGTTCCCT 
GTGCAGGACA TGAAGACTGG AGAGGGTGGC TTGCTTCAAG TCACCCTGGA AGGGATCAAT 
CTCAAGTTCA TGCACAACCA GTTCCTGAAA TTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106106.1
CDS	337..5001
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_030250246.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250246.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGTTCC 
GGAGAGACCC TTGGAGCTGA CAGTGACCTG AGCAGCAATG CAGGTGACGG GCCAGGAGGC 
GAAGGCAGCG CCCACCTGGC AAGTTCCCGG GCCACTCTGT CCGACAGTGA AATCGAAACC 
AATTCTGCTA CAAGCGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAA GGAGAAGCCG 
GCTGGTAGTC CGATTCGCTC TTCCGAAGAC GTAAGCCAGC GAGTCTATCT CTATGAGGGA 
CTGCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGCTAG AGGATGCCGC TATGGAGACC 
TTTTCTCTGA GCAAAGAACG TTCTACCTTA TGGGACCAAA TGCAGTTCTG GGAAGATGCA 
TTCTTAGATG CTGTAATGTT GGAAAGAGAA GGAATGGGTA TGGACCAGGG TCCTCAGGAA 
ATGATCGACA GGTACCTCTC CCTAGGGGAA CACGACCGGA AGCGCCTGGA GGATGATGAA 
GACCGCTTGC TGGCCACACT TTTACACAAC CTCATATCCT ATATGCTGCT GATGAAGGTG 
AACAAGAATG ACATCAGGAA GAAAGTACGG CGCCTAATGG GAAAGTCCCA CATTGGGCTG 
GTGTACAGCC AGCAAGTCAA TGAGGTGCTC GATCAGCTGA ACAGCCTGAA TGGACGTGAT 
CTCTCCATCC GGTCCAGCGG CAGCCGGCAC ATGAAGAAGC AGACTTTTGT TGTGCATGCA 
GGGACGGACA CAAATGGAGA CATCTTTTTC ATGGAAGTGT GTGATGACTG CGTGGTGTTG 
CGTAGTAACA TTGGGACGGT GTACGAGCGC TGGTGGTATG AGAAGCTCAT CAATATGACC 
TACTGTCCCA AGACCAAGGT CCTGTGCTTA TGGCGGAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACCAAGAA GTGTCGAGAG CTGTACTACT GTGTGAAGGA CAGCATGGAA 
CGTGCTGCTG CCCGACAGCA AAGCATCAAA CCTGGGCCTG AGCTAGGCGG TGAGTTCCCT 
GTGCAGGACA TGAAGACTGG AGAGGGTGGC TTGCTTCAAG TCACCCTGGA AGGGATCAAT 
CTCAAGTTCA TGCACAACCA GTTCCTGAAA TTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250249.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106109.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X39, mRNA.

Price & Availability↑

CloneID	OMu87842
Clone ID Related Accession (Same CDS sequence)	XM_030250249.1
Accession Version	XM_030250249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106109.1
CDS	337..4956
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_030250249.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250249.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_030250253.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106113.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X41, mRNA.

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CloneID	OMu87843
Clone ID Related Accession (Same CDS sequence)	XM_030250253.1
Accession Version	XM_030250253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X37
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GTATTTGGGC TAAATACTCT AATGGAGATT GTTACTGAAG CCGGCCCTGG GAGTGGTGAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGAGTAAT 
AGTTCCGGAG AGACCCTTGG AGCTGACAGT GACCTGAGCA GCAATGCAGG TGACGGGCCA 
GGAGGCGAAG GCAGCGCCCA CCTGGCAAGT TCCCGGGCCA CTCTGTCCGA CAGTGAAATC 
GAAACCAATT CTGCTACAAG CGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAAGGAG 
AAGCCGGCTG GTAGTCCGAT TCGCTCTTCC GAAGACGTAA GCCAGCGAGT CTATCTCTAT 
GAGGGACTGC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGCTAGAGGA TGCCGCTATG 
GAGACCTTTT CTCTGAGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106113.1
CDS	337..4887
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_030250253.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250253.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GTATTTGGGC TAAATACTCT AATGGAGATT GTTACTGAAG CCGGCCCTGG GAGTGGTGAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGAGTAAT 
AGTTCCGGAG AGACCCTTGG AGCTGACAGT GACCTGAGCA GCAATGCAGG TGACGGGCCA 
GGAGGCGAAG GCAGCGCCCA CCTGGCAAGT TCCCGGGCCA CTCTGTCCGA CAGTGAAATC 
GAAACCAATT CTGCTACAAG CGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAAGGAG 
AAGCCGGCTG GTAGTCCGAT TCGCTCTTCC GAAGACGTAA GCCAGCGAGT CTATCTCTAT 
GAGGGACTGC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGCTAGAGGA TGCCGCTATG 
GAGACCTTTT CTCTGAGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( NM_001177719.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171190.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant 10, mRNA.

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CloneID	OMu00757
Clone ID Related Accession (Same CDS sequence)	NM_001177719.1 , XM_017317626.1
Accession Version	NM_001177719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform 10
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK036347.1, BC063386.1 and AK122257.1. Transcript Variant: This variant (10) represents the longest transcript and encodes isoform 10. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122257.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGCTAGA GGATGCCGCT 
ATGGAGACCT TTTCTCTGAG CAAAGAACGT TCTACCTTAT GGGACCAAAT GCAGTTCTGG 
GAAGATGCAT TCTTAGATGC TGTAATGTTG GAAAGAGAAG GAATGGGTAT GGACCAGGGT 
CCTCAGGAAA TGATCGACAG GTACCTCTCC CTAGGGGAAC ACGACCGGAA GCGCCTGGAG 
GATGATGAAG ACCGCTTGCT GGCCACACTT TTACACAACC TCATATCCTA TATGCTGCTG 
ATGAAGGTGA ACAAGAATGA CATCAGGAAG AAAGTACGGC GCCTAATGGG AAAGTCCCAC 
ATTGGGCTGG TGTACAGCCA GCAAGTCAAT GAGGTGCTCG ATCAGCTGAA CAGCCTGAAT 
GGACGTGATC TCTCCATCCG GTCCAGCGGC AGCCGGCACA TGAAGAAGCA GACTTTTGTT 
GTGCATGCAG GGACGGACAC AAATGGAGAC ATCTTTTTCA TGGAAGTGTG TGATGACTGC 
GTGGTGTTGC GTAGTAACAT TGGGACGGTG TACGAGCGCT GGTGGTATGA GAAGCTCATC 
AATATGACCT ACTGTCCCAA GACCAAGGTC CTGTGCTTAT GGCGGAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACCAAGAAG TGTCGAGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAAC GTGCTGCTGC CCGACAGCAA AGCATCAAAC CTGGGCCTGA GCTAGGCGGT 
GAGTTCCCTG TGCAGGACAT GAAGACTGGA GAGGGTGGCT TGCTTCAAGT CACCCTGGAA 
GGGATCAATC TCAAGTTCAT GCACAACCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171190.1
CDS	337..5070
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001177719.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177719.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGCTAGA GGATGCCGCT 
ATGGAGACCT TTTCTCTGAG CAAAGAACGT TCTACCTTAT GGGACCAAAT GCAGTTCTGG 
GAAGATGCAT TCTTAGATGC TGTAATGTTG GAAAGAGAAG GAATGGGTAT GGACCAGGGT 
CCTCAGGAAA TGATCGACAG GTACCTCTCC CTAGGGGAAC ACGACCGGAA GCGCCTGGAG 
GATGATGAAG ACCGCTTGCT GGCCACACTT TTACACAACC TCATATCCTA TATGCTGCTG 
ATGAAGGTGA ACAAGAATGA CATCAGGAAG AAAGTACGGC GCCTAATGGG AAAGTCCCAC 
ATTGGGCTGG TGTACAGCCA GCAAGTCAAT GAGGTGCTCG ATCAGCTGAA CAGCCTGAAT 
GGACGTGATC TCTCCATCCG GTCCAGCGGC AGCCGGCACA TGAAGAAGCA GACTTTTGTT 
GTGCATGCAG GGACGGACAC AAATGGAGAC ATCTTTTTCA TGGAAGTGTG TGATGACTGC 
GTGGTGTTGC GTAGTAACAT TGGGACGGTG TACGAGCGCT GGTGGTATGA GAAGCTCATC 
AATATGACCT ACTGTCCCAA GACCAAGGTC CTGTGCTTAT GGCGGAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACCAAGAAG TGTCGAGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAAC GTGCTGCTGC CCGACAGCAA AGCATCAAAC CTGGGCCTGA GCTAGGCGGT 
GAGTTCCCTG TGCAGGACAT GAAGACTGGA GAGGGTGGCT TGCTTCAAGT CACCCTGGAA 
GGGATCAATC TCAAGTTCAT GCACAACCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( NM_001177720.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171191.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant 11, mRNA.

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CloneID	OMu00759
Clone ID Related Accession (Same CDS sequence)	XM_017317620.1 , XM_030250213.1 , NM_001177720.1 , XM_030250212.1
Accession Version	NM_001177720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform 11
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC063386.1 and BC042212.1. Transcript Variant: This variant (11) lacks one coding exon, has two additional coding exons, and uses an alternate splice site, compared to variant 10. The resulting isoform (11) is longer and differs at several internal portions and the C-terminus, compared to isoform 10. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171191.1
CDS	85..4968
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001177720.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177720.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_017317626.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173115.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu00757
Clone ID Related Accession (Same CDS sequence)	NM_001177719.1 , XM_017317626.1
Accession Version	XM_017317626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGCTAGA GGATGCCGCT 
ATGGAGACCT TTTCTCTGAG CAAAGAACGT TCTACCTTAT GGGACCAAAT GCAGTTCTGG 
GAAGATGCAT TCTTAGATGC TGTAATGTTG GAAAGAGAAG GAATGGGTAT GGACCAGGGT 
CCTCAGGAAA TGATCGACAG GTACCTCTCC CTAGGGGAAC ACGACCGGAA GCGCCTGGAG 
GATGATGAAG ACCGCTTGCT GGCCACACTT TTACACAACC TCATATCCTA TATGCTGCTG 
ATGAAGGTGA ACAAGAATGA CATCAGGAAG AAAGTACGGC GCCTAATGGG AAAGTCCCAC 
ATTGGGCTGG TGTACAGCCA GCAAGTCAAT GAGGTGCTCG ATCAGCTGAA CAGCCTGAAT 
GGACGTGATC TCTCCATCCG GTCCAGCGGC AGCCGGCACA TGAAGAAGCA GACTTTTGTT 
GTGCATGCAG GGACGGACAC AAATGGAGAC ATCTTTTTCA TGGAAGTGTG TGATGACTGC 
GTGGTGTTGC GTAGTAACAT TGGGACGGTG TACGAGCGCT GGTGGTATGA GAAGCTCATC 
AATATGACCT ACTGTCCCAA GACCAAGGTC CTGTGCTTAT GGCGGAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACCAAGAAG TGTCGAGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAAC GTGCTGCTGC CCGACAGCAA AGCATCAAAC CTGGGCCTGA GCTAGGCGGT 
GAGTTCCCTG TGCAGGACAT GAAGACTGGA GAGGGTGGCT TGCTTCAAGT CACCCTGGAA 
GGGATCAATC TCAAGTTCAT GCACAACCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173115.1
CDS	295..5028
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017317626.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317626.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGCTAGA GGATGCCGCT 
ATGGAGACCT TTTCTCTGAG CAAAGAACGT TCTACCTTAT GGGACCAAAT GCAGTTCTGG 
GAAGATGCAT TCTTAGATGC TGTAATGTTG GAAAGAGAAG GAATGGGTAT GGACCAGGGT 
CCTCAGGAAA TGATCGACAG GTACCTCTCC CTAGGGGAAC ACGACCGGAA GCGCCTGGAG 
GATGATGAAG ACCGCTTGCT GGCCACACTT TTACACAACC TCATATCCTA TATGCTGCTG 
ATGAAGGTGA ACAAGAATGA CATCAGGAAG AAAGTACGGC GCCTAATGGG AAAGTCCCAC 
ATTGGGCTGG TGTACAGCCA GCAAGTCAAT GAGGTGCTCG ATCAGCTGAA CAGCCTGAAT 
GGACGTGATC TCTCCATCCG GTCCAGCGGC AGCCGGCACA TGAAGAAGCA GACTTTTGTT 
GTGCATGCAG GGACGGACAC AAATGGAGAC ATCTTTTTCA TGGAAGTGTG TGATGACTGC 
GTGGTGTTGC GTAGTAACAT TGGGACGGTG TACGAGCGCT GGTGGTATGA GAAGCTCATC 
AATATGACCT ACTGTCCCAA GACCAAGGTC CTGTGCTTAT GGCGGAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACCAAGAAG TGTCGAGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAAC GTGCTGCTGC CCGACAGCAA AGCATCAAAC CTGGGCCTGA GCTAGGCGGT 
GAGTTCCCTG TGCAGGACAT GAAGACTGGA GAGGGTGGCT TGCTTCAAGT CACCCTGGAA 
GGGATCAATC TCAAGTTCAT GCACAACCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_017317620.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173109.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu00759
Clone ID Related Accession (Same CDS sequence)	XM_017317620.1 , XM_030250213.1 , NM_001177720.1 , XM_030250212.1
Accession Version	XM_017317620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173109.1
CDS	295..5178
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017317620.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317620.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAAGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCAATGG CCCACGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCCGCA GTTCGTAGCA GTGAAGAAGA CCTTCGAACC 
CCACCTCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_017317619.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173108.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X4, mRNA.

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CloneID	OMu30279
Clone ID Related Accession (Same CDS sequence)	XM_017317619.1 , XM_030250207.1
Accession Version	XM_017317619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173108.1
CDS	295..5229
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017317619.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317619.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_017317621.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173110.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X6, mRNA.

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CloneID	OMu30283
Clone ID Related Accession (Same CDS sequence)	XM_030250227.1 , XM_017317621.1
Accession Version	XM_017317621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAAG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCCCACG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC CGCAGTTCGT AGCAGTGAAG AAGACCTTCG AACCCCACCT 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173110.1
CDS	295..5112
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017317621.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317621.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAAG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCCCACG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC CGCAGTTCGT AGCAGTGAAG AAGACCTTCG AACCCCACCT 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_017317623.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173112.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X7, mRNA.

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CloneID	OMu30287
Clone ID Related Accession (Same CDS sequence)	XM_030250238.1 , XM_017317623.1
Accession Version	XM_017317623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAAG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCCCACG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC CGCAGTTCGT AGCAGTGAAG AAGACCTTCG AACCCCACCT 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173112.1
CDS	295..5052
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017317623.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317623.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCCGG AGAGACCCTT GGAGCTGACA GTGACCTGAG CAGCAATGCA 
GGTGACGGGC CAGGAGGCGA AGGCAGCGCC CACCTGGCAA GTTCCCGGGC CACTCTGTCC 
GACAGTGAAA TCGAAACCAA TTCTGCTACA AGCGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAAGG AGAAGCCGGC TGGTAGTCCG ATTCGCTCTT CCGAAGACGT AAGCCAGCGA 
GTCTATCTCT ATGAGGGACT GCTAGGCAAA GAACGTTCTA CCTTATGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTA ATGTTGGAAA GAGAAGGAAT GGGTATGGAC 
CAGGGTCCTC AGGAAATGAT CGACAGGTAC CTCTCCCTAG GGGAACACGA CCGGAAGCGC 
CTGGAGGATG ATGAAGACCG CTTGCTGGCC ACACTTTTAC ACAACCTCAT ATCCTATATG 
CTGCTGATGA AGGTGAACAA GAATGACATC AGGAAGAAAG TACGGCGCCT AATGGGAAAG 
TCCCACATTG GGCTGGTGTA CAGCCAGCAA GTCAATGAGG TGCTCGATCA GCTGAACAGC 
CTGAATGGAC GTGATCTCTC CATCCGGTCC AGCGGCAGCC GGCACATGAA GAAGCAGACT 
TTTGTTGTGC ATGCAGGGAC GGACACAAAT GGAGACATCT TTTTCATGGA AGTGTGTGAT 
GACTGCGTGG TGTTGCGTAG TAACATTGGG ACGGTGTACG AGCGCTGGTG GTATGAGAAG 
CTCATCAATA TGACCTACTG TCCCAAGACC AAGGTCCTGT GCTTATGGCG GAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACC AAGAAGTGTC GAGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAACGTGC TGCTGCCCGA CAGCAAAGCA TCAAACCTGG GCCTGAGCTA 
GGCGGTGAGT TCCCTGTGCA GGACATGAAG ACTGGAGAGG GTGGCTTGCT TCAAGTCACC 
CTGGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAAG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACGCCA ATGGCCCACG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC CGCAGTTCGT AGCAGTGAAG AAGACCTTCG AACCCCACCT 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_017317624.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173113.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu30288
Clone ID Related Accession (Same CDS sequence)	XM_017317624.1 , XM_030250240.1
Accession Version	XM_017317624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGAGTAATA GTTCCGGAGA GACCCTTGGA GCTGACAGTG ACCTGAGCAG CAATGCAGGT 
GACGGGCCAG GAGGCGAAGG CAGCGCCCAC CTGGCAAGTT CCCGGGCCAC TCTGTCCGAC 
AGTGAAATCG AAACCAATTC TGCTACAAGC GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAAGGAGA AGCCGGCTGG TAGTCCGATT CGCTCTTCCG AAGACGTAAG CCAGCGAGTC 
TATCTCTATG AGGGACTGCT AGGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173113.1
CDS	295..5049
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017317624.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317624.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGGG 
CCAGAGGGCA TCAAGCCTGT CTTTGACCTG GGTGAGACAG AGGAGAAAAA GTCCCAGATG 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GCCTCCCAGA GGACTGATCA AGACTCTGTC 
ATCGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGATTCTGA AGTTAGCACC 
GTGAGTAATA GTTCCGGAGA GACCCTTGGA GCTGACAGTG ACCTGAGCAG CAATGCAGGT 
GACGGGCCAG GAGGCGAAGG CAGCGCCCAC CTGGCAAGTT CCCGGGCCAC TCTGTCCGAC 
AGTGAAATCG AAACCAATTC TGCTACAAGC GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAAGGAGA AGCCGGCTGG TAGTCCGATT CGCTCTTCCG AAGACGTAAG CCAGCGAGTC 
TATCTCTATG AGGGACTGCT AGGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAAGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCAATG GCCCACGAGA TCTGCTACTC TGTGTTGTGT 
CTCTTCTCGT ATGTGGCCGC AGTTCGTAGC AGTGAAGAAG ACCTTCGAAC CCCACCTCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_017317625.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173114.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu30289
Clone ID Related Accession (Same CDS sequence)	XM_017317625.1 , XM_030250242.1
Accession Version	XM_017317625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173114.1
CDS	295..5031
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNPYFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017317625.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317625.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
TCAGTGTGCC AGCGAACCTA TGACAATCCA TACTTCGAGC CCCAGTATGG CTTTCCCCCC 
GAGGAAGATG AGGAGGAGCA GGGGGAGAGC TACACTCCCC GGTTCAGCCA GCATGTCAGT 
GGCAGTCGGG CTCAAAAGCT GCTGCGGCCC AACAGCTTGA AGCTGGCAAG TGACTCAGAC 
GCAGAGTCAG ACTCGAGAGC AAGCTCTCCC AACTCCACTG TCTCTAACAA CAGCACCGAG 
GGCTTCGGGG GCATCATGTC TTTTGCTAGC AGCCTGTATC GGAACCATAG TACAAGCTTC 
AGTCTTTCAA ACCTCACACT GCCCACCAAA GGTGCCCGCG AGAAGACGAC ACCATTCCCC 
AGCCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAATAGGAG GGCCTTAGTG GACCAGAAGT CATCTGTCAT TAAACACAGC 
CCAACCGTGA AAAGAGAACC TTCATCCCCA CAGGGCCGGT CGAGCAACTC TAGCGAGAAC 
CAGCAGTTCC TGAAGGAAGT AGTCCATAGT GTGCTGGACG GCCAGGGAGT GGGCTGGCTC 
AATATGAAGA AGGTACGCCG GCTCCTGGAG AGTGAGCAGC TTAGAGTCTT TGTACTGAGC 
AAGCTGAATC GCGCAGTGCA GTCAGAGGAT GACGCCCGGC AGGATGTCAT CCAAGACGTG 
GAGATCAGCC GGAAGGTGTA CAAGGGGATG CTAGACCTCC TCAAGTGCAC AGTCCTCAGC 
CTCGAGCAGT CCTATGCCCA CGCAGGTCTG GGTGGTATGG CCAGCATCTT TGGGCTTTTG 
GAGATTGCCC AGACCCACTA CTATAGTAAA GAACCAGACA AACGGAAGAG AAGTCCGACA 
GAGAATGTAA ATACCCCAGT TGGCAAGGAT CCTGGTCTGG CTGGGCGGGG GGACCCAAAG 
GCAATGGCCC AGCTAAGGGT CCCTCAACTG GGCCCTCGGG CACCAAGTGC TACAGGAAAG 
GGTCCTAAAG AACTGGATAC CAGAAGTTTA AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA 
TTGTGGAACA AGCACCAGGA AGTGAAAAAG CAAAAGGCTT TGGAAAAACA GAGGCCAGAG 
GGCATCAAGC CTGTCTTTGA CCTGGGTGAG ACAGAGGAGA AAAAGTCCCA GATGAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCTGCCTCC CAGAGGACTG ATCAAGACTC TGTCATCGGT 
GTGAGTCCAG CTGTTATGAT CCGAAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGTT CCGGAGAGAC CCTTGGAGCT GACAGTGACC TGAGCAGCAA TGCAGGTGAC 
GGGCCAGGAG GCGAAGGCAG CGCCCACCTG GCAAGTTCCC GGGCCACTCT GTCCGACAGT 
GAAATCGAAA CCAATTCTGC TACAAGCGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AAGGAGAAGC CGGCTGGTAG TCCGATTCGC TCTTCCGAAG ACGTAAGCCA GCGAGTCTAT 
CTCTATGAGG GACTGCTAGG AAGGGACAAA GGATCCATGT GGGACCAGCT AGAGGATGCC 
GCTATGGAGA CCTTTTCTCT GAGCAAAGAA CGTTCTACCT TATGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTAATG TTGGAAAGAG AAGGAATGGG TATGGACCAG 
GGTCCTCAGG AAATGATCGA CAGGTACCTC TCCCTAGGGG AACACGACCG GAAGCGCCTG 
GAGGATGATG AAGACCGCTT GCTGGCCACA CTTTTACACA ACCTCATATC CTATATGCTG 
CTGATGAAGG TGAACAAGAA TGACATCAGG AAGAAAGTAC GGCGCCTAAT GGGAAAGTCC 
CACATTGGGC TGGTGTACAG CCAGCAAGTC AATGAGGTGC TCGATCAGCT GAACAGCCTG 
AATGGACGTG ATCTCTCCAT CCGGTCCAGC GGCAGCCGGC ACATGAAGAA GCAGACTTTT 
GTTGTGCATG CAGGGACGGA CACAAATGGA GACATCTTTT TCATGGAAGT GTGTGATGAC 
TGCGTGGTGT TGCGTAGTAA CATTGGGACG GTGTACGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAATATGA CCTACTGTCC CAAGACCAAG GTCCTGTGCT TATGGCGGAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACCAAG AAGTGTCGAG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AACGTGCTGC TGCCCGACAG CAAAGCATCA AACCTGGGCC TGAGCTAGGC 
GGTGAGTTCC CTGTGCAGGA CATGAAGACT GGAGAGGGTG GCTTGCTTCA AGTCACCCTG 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_017317627.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173116.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X11, mRNA.

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CloneID	OMu30291
Clone ID Related Accession (Same CDS sequence)	XM_030250245.1 , XM_017317627.1
Accession Version	XM_017317627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GTATTTGGGC TAAATACTCT AATGGAGATT GTTACTGAAG CCGGCCCTGG GAGTGGTGAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTTC GAGGCGTCTT TGTCCTGGAA GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC AATGGCCCAC GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CCGCAGTTCG TAGCAGTGAA GAAGACCTTC GAACCCCACC TCGGCCTGTC 
TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173116.1
CDS	295..4983
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017317627.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317627.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GTATTTGGGC TAAATACTCT AATGGAGATT GTTACTGAAG CCGGCCCTGG GAGTGGTGAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTTC GAGGCGTCTT TGTCCTGGAA GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC AATGGCCCAC GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CCGCAGTTCG TAGCAGTGAA GAAGACCTTC GAACCCCACC TCGGCCTGTC 
TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_017317629.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173118.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X13, mRNA.

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CloneID	OMu30292
Clone ID Related Accession (Same CDS sequence)	XM_017317629.1 , XM_030250247.1
Accession Version	XM_017317629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTTC GAGGCGTCTT TGTCCTGGAA GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC AATGGCCCAC GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CCGCAGTTCG TAGCAGTGAA GAAGACCTTC GAACCCCACC TCGGCCTGTC 
TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173118.1
CDS	295..4923
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017317629.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317629.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTTC GAGGCGTCTT TGTCCTGGAA GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC AATGGCCCAC GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CCGCAGTTCG TAGCAGTGAA GAAGACCTTC GAACCCCACC TCGGCCTGTC 
TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_017317630.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173119.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X14, mRNA.

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CloneID	OMu30293
Clone ID Related Accession (Same CDS sequence)	XM_030250248.1 , XM_017317630.1
Accession Version	XM_017317630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGAGTAAT 
AGTTCCGGAG AGACCCTTGG AGCTGACAGT GACCTGAGCA GCAATGCAGG TGACGGGCCA 
GGAGGCGAAG GCAGCGCCCA CCTGGCAAGT TCCCGGGCCA CTCTGTCCGA CAGTGAAATC 
GAAACCAATT CTGCTACAAG CGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAAGGAG 
AAGCCGGCTG GTAGTCCGAT TCGCTCTTCC GAAGACGTAA GCCAGCGAGT CTATCTCTAT 
GAGGGACTGC TAGGCAAAGA ACGTTCTACC TTATGGGACC AAATGCAGTT CTGGGAAGAT 
GCATTCTTAG ATGCTGTAAT GTTGGAAAGA GAAGGAATGG GTATGGACCA GGGTCCTCAG 
GAAATGATCG ACAGGTACCT CTCCCTAGGG GAACACGACC GGAAGCGCCT GGAGGATGAT 
GAAGACCGCT TGCTGGCCAC ACTTTTACAC AACCTCATAT CCTATATGCT GCTGATGAAG 
GTGAACAAGA ATGACATCAG GAAGAAAGTA CGGCGCCTAA TGGGAAAGTC CCACATTGGG 
CTGGTGTACA GCCAGCAAGT CAATGAGGTG CTCGATCAGC TGAACAGCCT GAATGGACGT 
GATCTCTCCA TCCGGTCCAG CGGCAGCCGG CACATGAAGA AGCAGACTTT TGTTGTGCAT 
GCAGGGACGG ACACAAATGG AGACATCTTT TTCATGGAAG TGTGTGATGA CTGCGTGGTG 
TTGCGTAGTA ACATTGGGAC GGTGTACGAG CGCTGGTGGT ATGAGAAGCT CATCAATATG 
ACCTACTGTC CCAAGACCAA GGTCCTGTGC TTATGGCGGA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACCAA GAAGTGTCGA GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAACGTGCTG CTGCCCGACA GCAAAGCATC AAACCTGGGC CTGAGCTAGG CGGTGAGTTC 
CCTGTGCAGG ACATGAAGAC TGGAGAGGGT GGCTTGCTTC AAGTCACCCT GGAAGGGATC 
AATCTCAAGT TCATGCACAA CCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAAGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACGCCAAT GGCCCACGAG ATCTGCTACT CTGTGTTGTG TCTCTTCTCG 
TATGTGGCCG CAGTTCGTAG CAGTGAAGAA GACCTTCGAA CCCCACCTCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173119.1
CDS	295..4920
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017317630.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317630.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGAGTAAT 
AGTTCCGGAG AGACCCTTGG AGCTGACAGT GACCTGAGCA GCAATGCAGG TGACGGGCCA 
GGAGGCGAAG GCAGCGCCCA CCTGGCAAGT TCCCGGGCCA CTCTGTCCGA CAGTGAAATC 
GAAACCAATT CTGCTACAAG CGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAAGGAG 
AAGCCGGCTG GTAGTCCGAT TCGCTCTTCC GAAGACGTAA GCCAGCGAGT CTATCTCTAT 
GAGGGACTGC TAGGCAAAGA ACGTTCTACC TTATGGGACC AAATGCAGTT CTGGGAAGAT 
GCATTCTTAG ATGCTGTAAT GTTGGAAAGA GAAGGAATGG GTATGGACCA GGGTCCTCAG 
GAAATGATCG ACAGGTACCT CTCCCTAGGG GAACACGACC GGAAGCGCCT GGAGGATGAT 
GAAGACCGCT TGCTGGCCAC ACTTTTACAC AACCTCATAT CCTATATGCT GCTGATGAAG 
GTGAACAAGA ATGACATCAG GAAGAAAGTA CGGCGCCTAA TGGGAAAGTC CCACATTGGG 
CTGGTGTACA GCCAGCAAGT CAATGAGGTG CTCGATCAGC TGAACAGCCT GAATGGACGT 
GATCTCTCCA TCCGGTCCAG CGGCAGCCGG CACATGAAGA AGCAGACTTT TGTTGTGCAT 
GCAGGGACGG ACACAAATGG AGACATCTTT TTCATGGAAG TGTGTGATGA CTGCGTGGTG 
TTGCGTAGTA ACATTGGGAC GGTGTACGAG CGCTGGTGGT ATGAGAAGCT CATCAATATG 
ACCTACTGTC CCAAGACCAA GGTCCTGTGC TTATGGCGGA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACCAA GAAGTGTCGA GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAACGTGCTG CTGCCCGACA GCAAAGCATC AAACCTGGGC CTGAGCTAGG CGGTGAGTTC 
CCTGTGCAGG ACATGAAGAC TGGAGAGGGT GGCTTGCTTC AAGTCACCCT GGAAGGGATC 
AATCTCAAGT TCATGCACAA CCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAAGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACGCCAAT GGCCCACGAG ATCTGCTACT CTGTGTTGTG TCTCTTCTCG 
TATGTGGCCG CAGTTCGTAG CAGTGAAGAA GACCTTCGAA CCCCACCTCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_017317632.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173121.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X15, mRNA.

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CloneID	OMu30294
Clone ID Related Accession (Same CDS sequence)	XM_030250251.1 , XM_017317632.1
Accession Version	XM_017317632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTGAGTAA TAGTTCCGGA 
GAGACCCTTG GAGCTGACAG TGACCTGAGC AGCAATGCAG GTGACGGGCC AGGAGGCGAA 
GGCAGCGCCC ACCTGGCAAG TTCCCGGGCC ACTCTGTCCG ACAGTGAAAT CGAAACCAAT 
TCTGCTACAA GCGCCATCTT TGGTAAAGCC CATAGCTTGA AGCCAAAGGA GAAGCCGGCT 
GGTAGTCCGA TTCGCTCTTC CGAAGACGTA AGCCAGCGAG TCTATCTCTA TGAGGGACTG 
CTAGGCAAAG AACGTTCTAC CTTATGGGAC CAAATGCAGT TCTGGGAAGA TGCATTCTTA 
GATGCTGTAA TGTTGGAAAG AGAAGGAATG GGTATGGACC AGGGTCCTCA GGAAATGATC 
GACAGGTACC TCTCCCTAGG GGAACACGAC CGGAAGCGCC TGGAGGATGA TGAAGACCGC 
TTGCTGGCCA CACTTTTACA CAACCTCATA TCCTATATGC TGCTGATGAA GGTGAACAAG 
AATGACATCA GGAAGAAAGT ACGGCGCCTA ATGGGAAAGT CCCACATTGG GCTGGTGTAC 
AGCCAGCAAG TCAATGAGGT GCTCGATCAG CTGAACAGCC TGAATGGACG TGATCTCTCC 
ATCCGGTCCA GCGGCAGCCG GCACATGAAG AAGCAGACTT TTGTTGTGCA TGCAGGGACG 
GACACAAATG GAGACATCTT TTTCATGGAA GTGTGTGATG ACTGCGTGGT GTTGCGTAGT 
AACATTGGGA CGGTGTACGA GCGCTGGTGG TATGAGAAGC TCATCAATAT GACCTACTGT 
CCCAAGACCA AGGTCCTGTG CTTATGGCGG AGAAATGGCT CTGAGACCCA GCTCAACAAG 
TTCTATACCA AGAAGTGTCG AGAGCTGTAC TACTGTGTGA AGGACAGCAT GGAACGTGCT 
GCTGCCCGAC AGCAAAGCAT CAAACCTGGG CCTGAGCTAG GCGGTGAGTT CCCTGTGCAG 
GACATGAAGA CTGGAGAGGG TGGCTTGCTT CAAGTCACCC TGGAAGGGAT CAATCTCAAG 
TTCATGCACA ACCAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTTCGA 
GGCGTCTTTG TCCTGGAAGA ATTTGTTCCT GAAATTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCCCACGA GATCTGCTAC TCTGTGTTGT GTCTCTTCTC GTATGTGGCC 
GCAGTTCGTA GCAGTGAAGA AGACCTTCGA ACCCCACCTC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173121.1
CDS	295..4911
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017317632.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317632.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTGAGTAA TAGTTCCGGA 
GAGACCCTTG GAGCTGACAG TGACCTGAGC AGCAATGCAG GTGACGGGCC AGGAGGCGAA 
GGCAGCGCCC ACCTGGCAAG TTCCCGGGCC ACTCTGTCCG ACAGTGAAAT CGAAACCAAT 
TCTGCTACAA GCGCCATCTT TGGTAAAGCC CATAGCTTGA AGCCAAAGGA GAAGCCGGCT 
GGTAGTCCGA TTCGCTCTTC CGAAGACGTA AGCCAGCGAG TCTATCTCTA TGAGGGACTG 
CTAGGCAAAG AACGTTCTAC CTTATGGGAC CAAATGCAGT TCTGGGAAGA TGCATTCTTA 
GATGCTGTAA TGTTGGAAAG AGAAGGAATG GGTATGGACC AGGGTCCTCA GGAAATGATC 
GACAGGTACC TCTCCCTAGG GGAACACGAC CGGAAGCGCC TGGAGGATGA TGAAGACCGC 
TTGCTGGCCA CACTTTTACA CAACCTCATA TCCTATATGC TGCTGATGAA GGTGAACAAG 
AATGACATCA GGAAGAAAGT ACGGCGCCTA ATGGGAAAGT CCCACATTGG GCTGGTGTAC 
AGCCAGCAAG TCAATGAGGT GCTCGATCAG CTGAACAGCC TGAATGGACG TGATCTCTCC 
ATCCGGTCCA GCGGCAGCCG GCACATGAAG AAGCAGACTT TTGTTGTGCA TGCAGGGACG 
GACACAAATG GAGACATCTT TTTCATGGAA GTGTGTGATG ACTGCGTGGT GTTGCGTAGT 
AACATTGGGA CGGTGTACGA GCGCTGGTGG TATGAGAAGC TCATCAATAT GACCTACTGT 
CCCAAGACCA AGGTCCTGTG CTTATGGCGG AGAAATGGCT CTGAGACCCA GCTCAACAAG 
TTCTATACCA AGAAGTGTCG AGAGCTGTAC TACTGTGTGA AGGACAGCAT GGAACGTGCT 
GCTGCCCGAC AGCAAAGCAT CAAACCTGGG CCTGAGCTAG GCGGTGAGTT CCCTGTGCAG 
GACATGAAGA CTGGAGAGGG TGGCTTGCTT CAAGTCACCC TGGAAGGGAT CAATCTCAAG 
TTCATGCACA ACCAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTTCGA 
GGCGTCTTTG TCCTGGAAGA ATTTGTTCCT GAAATTAAAG AAGTGGTGAG CCACAAGTAC 
AAGACGCCAA TGGCCCACGA GATCTGCTAC TCTGTGTTGT GTCTCTTCTC GTATGTGGCC 
GCAGTTCGTA GCAGTGAAGA AGACCTTCGA ACCCCACCTC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madd ( XM_017317634.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173123.1 Mus musculus MAP-kinase activating death domain (Madd), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu73396
Clone ID Related Accession (Same CDS sequence)	XM_030250257.1 , XM_017317634.1
Accession Version	XM_017317634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	MAP kinase-activating death domain protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173123.1
CDS	295..4725
Translation	MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGSTLQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSSSSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVGPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPKEKPAGSPIRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017317634.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP-kinase activating death domain (Madd), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317634.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGTTCTG CCCTCGGTTA CTTGACTATC TCGTGATCGT AGGGGCGAGG 
CACCCAAGCA GTGACAGTGT GGCTCAGACT CCTGAGCTGC TGCGGAGGTA CCCACTAGAG 
GATCACCCAG AGTTCCCCCT GCCCCCAGAT GTGGTGTTCT TCTGCCAGCC AGAAGGATGT 
CTGAGTGTGC GGCAGCGGCG GATGAGCCTT CGGGACGATA CCTCTTTTGT CTTCACCCTA 
ACCGATAAGG ACACCGGAGT CACCCGCTAT GGCATCTGTG TCAACTTCTA CCGTTCCTTC 
CAAAAGCGAA TGCCAAAGGA AAAGGTGGAA GGCGGAGCAG GACCCCGTGG GAAGGAAGGC 
GCTCACACCT CTGGTGCCTC AGAAGAGGCT GCCGCTGGGA GCTCAGAGAG TGGCTCAACC 
TTGCAGCCGC CTAGTGCTGA CTCCACTCCT GACGTAAACC AGTCTCCTCG GGGCAAACGT 
AGGGCAAAAG CGGGCAGCCG CTCCCGCAAC AGTACCCTGA CATCCCTGTG TGTGCTTAGC 
CACTACCCTT TCTTCTCTAC CTTCAGAGAG TGTCTGTATA CTCTCAAACG TTTGGTAGAC 
TGCTGTAGTG AACGGCTGCT AGGCAAGAAA CTGGGCATCC CTCGAGGTGT ACAAAGGGAC 
ACCATGTGGC GAATCTTTAC TGGATCACTG CTAGTGGAGG AGAAGTCCAG TGCCCTTCTG 
CAAGACCTTC GAGAGATTGA GGCCTGGATC TATCGGTTGC TACGTTCCCC AGTGCCTGTC 
TCTGGGCAGA AGCGAGTGGA CATTGAGGTC CTGCCCCAGG AACTCCAGCA GGCCCTGACA 
TTTGCTCTTC CAGATCCATC TCGATTCACC CTAGTAGATT TCCCTCTTCA CCTTCCCTTG 
GAACTTCTGG GTGTAGATGC TTGTCTTCAG GTGCTAACCT GCATCCTGTT GGAGCACAAG 
GTGGTGCTGC AGTCTCGAGA CTACAATGCA CTCTCCATGT CTGTGATGGC GTTCGTGGCA 
ATGATCTACC CCCTGGAGTA CATGTTCCCG GTCATCCCGC TGCTTCCCAC CTGCATGGCC 
TCGGCAGAGC AGCTACTCTT GGCTCCAACC CCATACATCA TTGGAGTTCC TGCCAGCTTC 
TTCCTCTACA AGCTAGATTT CAAAATGCCC GATGACGTGT GGCTAGTGGA TCTGGACAGC 
AATAGGGTGA TTGCCCCCAC CAATGCAGAA GTGCTACCCA TCCTGCCAGA ACCAGAATCA 
TTAGAGCTGA AAAAACACTT AAAGCAGGCC TTAGCCAGCA TGAGTCTCAA CACCCAGCCC 
ATTCTTAACC TGGAGAAATT CCACGAGGGC CAGGAGATCC CGCTTCTCTT GGGAAGGCCA 
TCTAATGACC TTCAGTCCAC ACCTTCCACT GAATTCAACC CGCTCATTTA TGGCAATGAT 
GTAGATTCAG TCGACGTTGC AACGAGAGTG GCCATGGTAC GTTTCTTCAA CTCTGCTAAC 
GTGCTGCAGG GCTTTCAGAT GCACACACGT ACCCTGCGAC TCTTCCCCCG GCCTGTGGTA 
GCTTTCCAAG CTGGCTCCTT TCTGGCCTCA CGTCCCCGGC AGACTCCCTT TGCTGAGAAA 
CTGGCCAGGA CCCAAGCTGT GGAGTATTTT GGAGAATGGA TCCTGAACCC TTCTAACTAT 
GCCTTCCAGC GGATTCACAA CAATACGTTT GATCCGGCCC TTATTGGTGA CAAGCCAAAG 
TGGTACGCTC ACCAGCTGCA GCCCATCCAT TATCGAGTCT ATGATGGCAA CTCTCAGCTG 
GCCGAGGCCC TGAGTGTGCC CCCGGAGCGC GATTCTGACT CCGACCCGAC GGAGGACAGT 
GGCAGTGATA GTCAGGATTA TGATGACTCA AGCTCTTCTT ACTCCTCCCT TGGGGACTTT 
GTCAGTGAAA TGATGAAATG TGACATCAAT GGAGACACTC CCAATGTGGA TCCTCTGACC 
CATGCAGCCC TGGGAGATGC CAGTGAAGTG GAGATTGACG AGCTGCAGCC CCAGAAGGAG 
GGAGAGGAAC CCGGCCCAGA CAGTGAGAAC TCTCAGGAAA ACCCTCCGCT GCGCTCCAGC 
TCCAGCACCA CGGCCAGCAG CAGCCCCAGC ACCGTTGTCC ACAGTGCCCA CTCTGAAGCT 
GCTGACTCTA CAGAGATGGG AGATAAGGCC ACAGCAGGCA TCTCCAAGCC CCTCCCTCCC 
GTGCCTCCCA GCATTTGCAA ATCTACTGTG GACAGGAGAC AGACAGAGAC TGGAGAGGGG 
GCTCAAAAGC TGCTGCGGCC CAACAGCTTG AAGCTGGCAA GTGACTCAGA CGCAGAGTCA 
GACTCGAGAG CAAGCTCTCC CAACTCCACT GTCTCTAACA ACAGCACCGA GGGCTTCGGG 
GGCATCATGT CTTTTGCTAG CAGCCTGTAT CGGAACCATA GTACAAGCTT CAGTCTTTCA 
AACCTCACAC TGCCCACCAA AGGTGCCCGC GAGAAGACGA CACCATTCCC CAGCCTGAAA 
GGAAATAGGA GGGCCTTAGT GGACCAGAAG TCATCTGTCA TTAAACACAG CCCAACCGTG 
AAAAGAGAAC CTTCATCCCC ACAGGGCCGG TCGAGCAACT CTAGCGAGAA CCAGCAGTTC 
CTGAAGGAAG TAGTCCATAG TGTGCTGGAC GGCCAGGGAG TGGGCTGGCT CAATATGAAG 
AAGGTACGCC GGCTCCTGGA GAGTGAGCAG CTTAGAGTCT TTGTACTGAG CAAGCTGAAT 
CGCGCAGTGC AGTCAGAGGA TGACGCCCGG CAGGATGTCA TCCAAGACGT GGAGATCAGC 
CGGAAGGTGT ACAAGGGGAT GCTAGACCTC CTCAAGTGCA CAGTCCTCAG CCTCGAGCAG 
TCCTATGCCC ACGCAGGTCT GGGTGGTATG GCCAGCATCT TTGGGCTTTT GGAGATTGCC 
CAGACCCACT ACTATAGTAA AGAACCAGAC AAACGGAAGA GAAGTCCGAC AGAGAATGTA 
AATACCCCAG TTGGCAAGGA TCCTGGTCTG GCTGGGCGGG GGGACCCAAA GGCAATGGCC 
CAGCTAAGGG TCCCTCAACT GGGCCCTCGG GCACCAAGTG CTACAGGAAA GGGTCCTAAA 
GAACTGGATA CCAGAAGTTT AAAGGAAGAG AATTTTGTAG CATCTGTTGG GCCAGAGGGC 
ATCAAGCCTG TCTTTGACCT GGGTGAGACA GAGGAGAAAA AGTCCCAGAT GAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGCCTCCCAG AGGACTGATC AAGACTCTGT CATCGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGATTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCCG GAGAGACCCT TGGAGCTGAC AGTGACCTGA GCAGCAATGC AGGTGACGGG 
CCAGGAGGCG AAGGCAGCGC CCACCTGGCA AGTTCCCGGG CCACTCTGTC CGACAGTGAA 
ATCGAAACCA ATTCTGCTAC AAGCGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAAG 
GAGAAGCCGG CTGGTAGTCC GATTCGCTCT TCCGAAGACG TAAGCCAGCG AGTCTATCTC 
TATGAGGGAC TGCTAGGCAA AGAACGTTCT ACCTTATGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT AATGTTGGAA AGAGAAGGAA TGGGTATGGA CCAGGGTCCT 
CAGGAAATGA TCGACAGGTA CCTCTCCCTA GGGGAACACG ACCGGAAGCG CCTGGAGGAT 
GATGAAGACC GCTTGCTGGC CACACTTTTA CACAACCTCA TATCCTATAT GCTGCTGATG 
AAGGTGAACA AGAATGACAT CAGGAAGAAA GTACGGCGCC TAATGGGAAA GTCCCACATT 
GGGCTGGTGT ACAGCCAGCA AGTCAATGAG GTGCTCGATC AGCTGAACAG CCTGAATGGA 
CGTGATCTCT CCATCCGGTC CAGCGGCAGC CGGCACATGA AGAAGCAGAC TTTTGTTGTG 
CATGCAGGGA CGGACACAAA TGGAGACATC TTTTTCATGG AAGTGTGTGA TGACTGCGTG 
GTGTTGCGTA GTAACATTGG GACGGTGTAC GAGCGCTGGT GGTATGAGAA GCTCATCAAT 
ATGACCTACT GTCCCAAGAC CAAGGTCCTG TGCTTATGGC GGAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC CAAGAAGTGT CGAGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAACGTG CTGCTGCCCG ACAGCAAAGC ATCAAACCTG GGCCTGAGCT AGGCGGTGAG 
TTCCCTGTGC AGGACATGAA GACTGGAGAG GGTGGCTTGC TTCAAGTCAC CCTGGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

IG20/MADD plays a critical role in glucose-induced insulin secretion.
Diabetes63(5)1612-23(2014 May)
Li LC,Wang Y,Carr R,Haddad CS,Li Z,Qian L,Oberholzer J,Maker AV,Wang Q,Prabhakar BS

A mutation in the Srrm4 gene causes alternative splicing defects and deafness in the Bronx waltzer mouse.
PLoS genetics8(10)e1002966(2012)
Nakano Y,Jahan I,Bonde G,Sun X,Hildebrand MS,Engelhardt JF,Smith RJ,Cornell RA,Fritzsch B,B?nfi B

KIF1Bbeta- and KIF1A-mediated axonal transport of presynaptic regulator Rab3 occurs in a GTP-dependent manner through DENN/MADD.
Nature cell biology10(11)1269-79(2008 Nov)
Niwa S,Tanaka Y,Hirokawa N

Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.
Molecular & cellular proteomics : MCP6(2)283-93(2007 Feb)
Munton RP,Tweedie-Cullen R,Livingstone-Zatchej M,Weinandy F,Waidelich M,Longo D,Gehrig P,Potthast F,Rutishauser D,Gerrits B,Panse C,Schlapbach R,Mansuy IM

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Madd cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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