Kcnt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnt1
Entrez Gene ID	227632
Full Name	potassium channel, subfamily T, member 1
Synonyms	C030030G16Rik,Slack,Slo2.2,slo2
General protein information	Preferred Names potassium channel, subfamily T, member 1 Names potassium channel subfamily T member 1 potassium channel subunit homolog sodium-activated potassium channel
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 A3
Summary	This gene encodes a member of the Slo potassium channel family that has shown to be activated by both sodium and chloride ions. This channel represents the largest potassium channel subunit yet identified. This channel may be important in development and pain signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006497878.3	XP_006497941.2	potassium channel subfamily T member 1 isoform X2
XM_006497886.3	XP_006497949.2	potassium channel subfamily T member 1 isoform X10
XM_006497895.3	XP_006497958.2	potassium channel subfamily T member 1 isoform X21
XM_006497878.4	XP_006497941.2	potassium channel subfamily T member 1 isoform X2
XM_017317198.2	XP_017172687.1	potassium channel subfamily T member 1 isoform X14
XM_030249674.1	XP_030105534.1	potassium channel subfamily T member 1 isoform X17
XM_006497894.4	XP_006497957.2	potassium channel subfamily T member 1 isoform X22
XM_030249700.1	XP_030105560.1	potassium channel subfamily T member 1 isoform X26
XM_006497877.3	XP_006497940.2	potassium channel subfamily T member 1 isoform X1
XM_006497891.3	XP_006497954.1	potassium channel subfamily T member 1 isoform X14
XM_006497879.4	XP_006497942.2	potassium channel subfamily T member 1 isoform X3
XM_006497880.4	XP_006497943.2	potassium channel subfamily T member 1 isoform X4
XM_006497881.4	XP_006497944.2	potassium channel subfamily T member 1 isoform X5
XM_006497884.4	XP_006497947.2	potassium channel subfamily T member 1 isoform X10
XM_006497891.4	XP_006497954.1	potassium channel subfamily T member 1 isoform X16
XM_006497889.4	XP_006497952.2	potassium channel subfamily T member 1 isoform X18
XM_006497896.4	XP_006497959.1	potassium channel subfamily T member 1 isoform X25
XM_006497879.3	XP_006497942.2	potassium channel subfamily T member 1 isoform X3
XM_006497882.3	XP_006497945.2	potassium channel subfamily T member 1 isoform X6
XM_006497884.3	XP_006497947.2	potassium channel subfamily T member 1 isoform X8
XM_006497889.3	XP_006497952.2	potassium channel subfamily T member 1 isoform X15
XM_006497894.3	XP_006497957.2	potassium channel subfamily T member 1 isoform X19
XM_017317210.1	XP_017172699.1	potassium channel subfamily T member 1 isoform X25
XM_030249649.1	XP_030105509.1	potassium channel subfamily T member 1 isoform X8
XM_030249677.1	XP_030105537.1	potassium channel subfamily T member 1 isoform X19
XM_006497893.4	XP_006497956.1	potassium channel subfamily T member 1 isoform X21
XM_030249694.1	XP_030105554.1	potassium channel subfamily T member 1 isoform X25
XM_006497880.3	XP_006497943.2	potassium channel subfamily T member 1 isoform X4
XM_017317195.1	XP_017172684.1	potassium channel subfamily T member 1 isoform X9
XM_017317196.1	XP_017172685.1	potassium channel subfamily T member 1 isoform X11
XM_017317199.1	XP_017172688.1	potassium channel subfamily T member 1 isoform X16
XM_017317204.1	XP_017172693.1	potassium channel subfamily T member 1 isoform X20
XM_017317206.1	XP_017172695.1	potassium channel subfamily T member 1 isoform X22
XM_017317207.1	XP_017172696.1	potassium channel subfamily T member 1 isoform X24
XM_006497877.4	XP_006497940.2	potassium channel subfamily T member 1 isoform X1
XM_006497883.4	XP_006497946.2	potassium channel subfamily T member 1 isoform X9
XM_006497892.4	XP_006497955.1	potassium channel subfamily T member 1 isoform X20
XM_006497895.4	XP_006497958.2	potassium channel subfamily T member 1 isoform X23
XM_030249688.1	XP_030105548.1	potassium channel subfamily T member 1 isoform X24
XM_030249691.1	XP_030105551.1	potassium channel subfamily T member 1 isoform X24
XM_017317210.2	XP_017172699.1	potassium channel subfamily T member 1 isoform X27
NM_001145403.2	NP_001138875.1	potassium channel subfamily T member 1 isoform 2
NM_175462.4	NP_780671.2	potassium channel subfamily T member 1 isoform 1
XM_006497881.3	XP_006497944.2	potassium channel subfamily T member 1 isoform X5
XM_006497883.3	XP_006497946.2	potassium channel subfamily T member 1 isoform X7
XM_017317197.1	XP_017172686.1	potassium channel subfamily T member 1 isoform X12
XM_017317198.1	XP_017172687.1	potassium channel subfamily T member 1 isoform X13
XM_006497892.3	XP_006497955.1	potassium channel subfamily T member 1 isoform X17
XM_006497893.3	XP_006497956.1	potassium channel subfamily T member 1 isoform X18
XM_006497896.3	XP_006497959.1	potassium channel subfamily T member 1 isoform X23
XM_006497882.4	XP_006497945.2	potassium channel subfamily T member 1 isoform X6
XM_030249645.1	XP_030105505.1	potassium channel subfamily T member 1 isoform X7
XM_017317195.2	XP_017172684.1	potassium channel subfamily T member 1 isoform X11
XM_006497886.4	XP_006497949.2	potassium channel subfamily T member 1 isoform X12
XM_017317197.2	XP_017172686.1	potassium channel subfamily T member 1 isoform X13
XM_030249663.1	XP_030105523.1	potassium channel subfamily T member 1 isoform X15
NM_001302351.1	NP_001289280.1	potassium channel subfamily T member 1 isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	KCNT1	NP_065873.2
Caenorhabditis elegans (roundworm)	slo-2	NP_001129924.1
Xenopus tropicalis (tropical clawed frog)	kcnt2	NP_001163984.2
	LOC721830	XP_001118025.2
Mus musculus (house mouse)	Kcnt1	NP_780671.2
Pan troglodytes (chimpanzee)	KCNT1	XP_003312453.2
Canis lupus familiaris (dog)	KCNT1	XP_005625191.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007585	XP_001687885.2
Drosophila melanogaster (fruit fly)	CG42732	NP_001097259.2
Bos taurus (cattle)	KCNT1	XP_002691660.3
Rattus norvegicus (Norway rat)	Kcnt1	NP_068625.1
Gallus gallus (chicken)	KCNT1	NP_989893.1
Danio rerio (zebrafish)	kcnt1	XP_001920632.3

Gene Ontology
Bibliography

Related articles in PubMed

Differential distribution of the sodium-activated potassium channels slick and slack in mouse brain.
Rizzi S, Knaus HG, Schwarzer C
The Journal of comparative neurology524(10)2093-116(2016 Jul)

Knockout of Slo2.2 enhances itch, abolishes KNa current, and increases action potential firing frequency in DRG neurons.
Martinez-Espinosa PL, Wu J, Yang C, Gonzalez-Perez V, Zhou H, Liang H, Xia XM, Lingle CJ
eLife4(2015 Nov)

FoxP1 orchestration of ASD-relevant signaling pathways in the striatum.
Araujo DJ, Anderson AG, Berto S, Runnels W, Harper M, Ammanuel S, Rieger MA, Huang HC, Rajkovich K, Loerwald KW, Dekker JD, Tucker HO, Dougherty JD, Gibson JR, Konopka G
Genes & development29(20)2081-96(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The highest Slack channel expression was detected in the olfactory bulb, lateral septal nuclei, basal ganglia, and distinct areas of the midbrain, brainstem, and cerebellar cortex.
Title: Differential distribution of the sodium-activated potassium channels slick and slack in mouse brain.

The authors find that knockout of Slo2.2, but not Slo2.1, results in enhanced itch and pain responses.
Title: Knockout of Slo2.2 enhances itch, abolishes KNa current, and increases action potential firing frequency in DRG neurons.

Results provide evidence for a role for endogenous Slack channels in higher brain functions, i.e., learning and memory, cognitive flexibility, locomotoric and the ability to initially respond to novel situations and environments
Title: The sodium-activated potassium channel Slack is required for optimal cognitive flexibility in mice.

Global ablation of Slack led to increased hypersensitivity in models of neuropathic pain. Neuropathic pain behaviors were also exaggerated after ablation of Slack selectively in sensory neurons.
Title: Slack channels expressed in sensory neurons control neuropathic pain in mice.

Slick and Slack are expressed at high levels auditory brainstem. Activation of these KNa channels allows temporal accuracy of firing to be increased at high frequencies of stimulation.
Title: Slack and Slick K(Na) channels regulate the accuracy of timing of auditory neurons.

The following Kcnt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu29456	XM_006497896.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu29456	XM_030249694.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu73356	XM_030249677.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu73357	XM_006497894.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu73358	XM_006497895.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu80190	XM_017317204.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu80191	XM_017317206.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu80192	XM_017317207.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu80193	XM_017317210.2 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu87791	XM_030249691.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu87791	XM_030249688.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu87792	XM_030249700.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu29451	XM_006497891.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu29452	XM_006497892.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu29453	XM_006497893.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu73346	XM_006497877.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu73347	XM_006497878.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu73348	XM_006497879.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu73349	XM_006497880.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu73350	XM_006497881.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu73351	XM_006497882.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu73352	XM_006497883.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu73353	XM_006497884.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu73354	XM_006497886.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu73355	XM_006497889.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu80185	XM_017317195.2 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu80186	XM_017317196.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu80187	XM_017317197.2 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu80188	XM_017317198.2 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu80189	XM_017317199.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87787	XM_030249645.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu87788	XM_030249649.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu87789	XM_030249663.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87790	XM_030249674.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu05439	NM_175462.4 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$727.30 $1039.00
OMu06861	NM_001145403.2 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu73345	NM_001302351.1 Latest version!	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu73347	XM_006497878.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu29451	XM_006497891.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu73348	XM_006497879.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu73353	XM_006497884.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu73355	XM_006497889.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu73357	XM_006497894.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu73349	XM_006497880.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu73346	XM_006497877.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu73358	XM_006497895.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu80193	XM_017317210.1	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu73350	XM_006497881.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu73352	XM_006497883.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu80188	XM_017317198.1	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu29452	XM_006497892.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu29453	XM_006497893.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu29456	XM_006497896.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu73351	XM_006497882.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu80185	XM_017317195.1	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu73354	XM_006497886.3	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu80187	XM_017317197.1	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnt1 ( XM_006497896.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497959.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X26, mRNA.

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CloneID	OMu29456
Clone ID Related Accession (Same CDS sequence)	XM_006497896.4 , XM_030249694.1 , XM_006497896.3
Accession Version	XM_006497896.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497896.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGAA GAGTGCAAGT ACGCCATGCT GGCCCTGAAC TGCATCTGCC CGGCCACCTC 
CACCCTCATC ACCCTGCTGG TGCACACGTC CCGTGGCCAG TGAGTGCCCT GTGCCAGACC 
GACTACCACG CCCTGGTCAT CCCGATCAAC CCCACAAGGG AAGGACAGGA GTCTCCCGAG 
CAGTGGCAGC GCACGTACGG GAGGTGCTCG GGCAACGAGG TGTACCACAT TCGCATGGGT 
GACAGTAAAT TCTTCCGGGA GTATGAGGGC AAGAGCTTCA CCTACGCAGC CTTCCACGCG 
CACAAGAAAT ATGGGGTGTG CCTCATCGGG CTGAAGCGTG AGGAGAACAA GAGTATCCTG 
CTGAACCCAG GACCACGGCA CATCCTGGCT GCCTCCGACA CCTGCTTCTA TATCAATATT 
ACCAAGGAGG AGAACTCAGC TTTCATCTTC AAACAGGAGG AGAAGCAGAA GAGACGGGGC 
CTTGCAGGGC AGGCACTATA TGAAGGGCCC TCCCGGCTCC CAGTGCATAG CATCATCGCC 
TCTATGGGGA CAGTGGCCAT GGACCTGCAG AACACAGATT GCCGGCCCTC CCAGGGTGGC 
AGTGGCGGGG ACGGCACAAA GCTGACTCTG CCCACCGAGA ACGGCTCTGG TAGTCGACGC 
CCCAGCATCG CACCCGTTCT GGAGTTGGCA GACAGCTCAG CCCTGTTGCC CTGCGACCTG 
CTGAGTGACC AGTCAGAGGA TGAGGTGACA CCCTCGGACG ACGAGGGGCT CTCTGTGGTT 
GAGTACGTGA AGGGCTATCC CCCCAACTCA CCCTACATTG GCAGCTCCCC GACTTTATGC 
CACCTCCTGC CTGTGAAAGC CCCCTTCTGC TGCCTGCGGT TGGACAAGGG CTGCAAACAC 
AACAGCTACG AGGATGCCAA GGCCTATGGG TTCAAGAACA AGCTGATTAT TGTCTCTGCT 
GAGACGGCAG GCAACGGGCT CTACAACTTC ATCGTGCCTC TGCGCGCCTA CTACCGGTCC 
CGCCGGGAGC TCAACCCTAT CGTGTTGCTG CTTGACAACA AGCCTGACCA CCACTTCCTG 
GAGGCCATCT GTTGCTTCCC CATGGTCTAC TACATGGAGG GATCCGTGGA CAACCTGGAC 
AGCTTACTGC AGTGTGGCAT CATCTATGCT GACAACCTGG TGGTGGTGGA CAAGGAGAGC 
ACTATGAGCG CTGAAGAGGA CTACATGGCA GATGCCAAGA CCATCGTCAA TGTGCAAACC 
ATGTTCCGGC TTTTCCCCAG TCTCAGCATC ACCACGGAGC TCACACACCC TTCCAACATG 
CGGTTCATGC AGTTCCGTGC CAAGGACAGC TACTCTCTGG CTCTTTCCAA ACTTGAAAAG 
CAAGAACGGG AGAACGGCTC CAACCTGGCC TTCATGTTCC GCCTGCCATT TGCTGCTGGT 
CGAGTATTTA GTATCAGCAT GTTGGATACA CTGCTCTACC AGTCCTTCGT GAAGGACTAC 
ATGATCACCA TCACCAGGCT GCTCTTGGGC CTTGATACTA CACCAGGCTC CGGCTACCTC 
TGTGCAATGA AGGTAACCGA GGACGACCTG TGGATCCGCA CTTACGGCCG CCTCTTCCAG 
AAACTCTGCT CCTCCAGCGC CGAGATCCCC ATCGGCATCT ACAGGACCGA GTGCCATGTC 
TTCTCGGAGC CCCATGACGT CAGAGCCCAG GGGGGCCCAG ACATAGCCCT CCTGCCTGCC 
CAGTCTCAGA TCTCGGTGAA CATGGAGGAC TGCGAGGATA CTCGGGAGGC CAAGGGACCC 
TGGGGCACAC GAGCTGCATC TGGCAGTGGC AGCACCCATG GCCGTCACGG GGGCAGTGCT 
GACCCAGTGG AGCACCCACT ACTACGTCGC AAGAGCCTGC AGTGGGCCCG CAAGCTGAGT 
CGCAAGAGCA CCAAGCAGGC AGGGAAGGCA CCTGTGGCCA CAGACTGGAT CACCCAGCAG 
CGGCTCAGCC TGTACCGACG CTCAGAGCGC CAGGAGCTCT CAGAGCTGGT CAAGAACCGA 
ATGAAGCACC TGGGACTGCC CACCACTGGC TATGAGGACG TAGCAAATTT AACAGCCAGT 
GATGTGATGA ATCGGGTAAA CCTGGGATAT TTGCAAGATG AGATGAACGA TCATCACCAG 
AACACCCTTT CCTATGTACT CATCAACCCC CCGCCAGACA CAAGACTGGA ACCCAACGAC 
ATTGTGTACC TCATCCGTTC CGACCCCCTG GCCCATGTGG CCAGCAGCTC CCAGAGTCGG 
AAAAGCAGCT GCAGCAACAA GCTCTCATCC TGTAATCCTG AGACCAGGGA TGAGACCCAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497959.1
CDS	603..2948
Translation	MRGRVQVRHAGPELHLPGHLHPHHPAGAHVPWPVSALCQTDYHALVIPINPTREGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497896.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497896.3

ORF Insert Sequence:

ATGCGAGGAA GAGTGCAAGT ACGCCATGCT GGCCCTGAAC TGCATCTGCC CGGCCACCTC 
CACCCTCATC ACCCTGCTGG TGCACACGTC CCGTGGCCAG TGAGTGCCCT GTGCCAGACC 
GACTACCACG CCCTGGTCAT CCCGATCAAC CCCACAAGGG AAGGACAGGA GTCTCCCGAG 
CAGTGGCAGC GCACGTACGG GAGGTGCTCG GGCAACGAGG TGTACCACAT TCGCATGGGT 
GACAGTAAAT TCTTCCGGGA GTATGAGGGC AAGAGCTTCA CCTACGCAGC CTTCCACGCG 
CACAAGAAAT ATGGGGTGTG CCTCATCGGG CTGAAGCGTG AGGAGAACAA GAGTATCCTG 
CTGAACCCAG GACCACGGCA CATCCTGGCT GCCTCCGACA CCTGCTTCTA TATCAATATT 
ACCAAGGAGG AGAACTCAGC TTTCATCTTC AAACAGGAGG AGAAGCAGAA GAGACGGGGC 
CTTGCAGGGC AGGCACTATA TGAAGGGCCC TCCCGGCTCC CAGTGCATAG CATCATCGCC 
TCTATGGGGA CAGTGGCCAT GGACCTGCAG AACACAGATT GCCGGCCCTC CCAGGGTGGC 
AGTGGCGGGG ACGGCACAAA GCTGACTCTG CCCACCGAGA ACGGCTCTGG TAGTCGACGC 
CCCAGCATCG CACCCGTTCT GGAGTTGGCA GACAGCTCAG CCCTGTTGCC CTGCGACCTG 
CTGAGTGACC AGTCAGAGGA TGAGGTGACA CCCTCGGACG ACGAGGGGCT CTCTGTGGTT 
GAGTACGTGA AGGGCTATCC CCCCAACTCA CCCTACATTG GCAGCTCCCC GACTTTATGC 
CACCTCCTGC CTGTGAAAGC CCCCTTCTGC TGCCTGCGGT TGGACAAGGG CTGCAAACAC 
AACAGCTACG AGGATGCCAA GGCCTATGGG TTCAAGAACA AGCTGATTAT TGTCTCTGCT 
GAGACGGCAG GCAACGGGCT CTACAACTTC ATCGTGCCTC TGCGCGCCTA CTACCGGTCC 
CGCCGGGAGC TCAACCCTAT CGTGTTGCTG CTTGACAACA AGCCTGACCA CCACTTCCTG 
GAGGCCATCT GTTGCTTCCC CATGGTCTAC TACATGGAGG GATCCGTGGA CAACCTGGAC 
AGCTTACTGC AGTGTGGCAT CATCTATGCT GACAACCTGG TGGTGGTGGA CAAGGAGAGC 
ACTATGAGCG CTGAAGAGGA CTACATGGCA GATGCCAAGA CCATCGTCAA TGTGCAAACC 
ATGTTCCGGC TTTTCCCCAG TCTCAGCATC ACCACGGAGC TCACACACCC TTCCAACATG 
CGGTTCATGC AGTTCCGTGC CAAGGACAGC TACTCTCTGG CTCTTTCCAA ACTTGAAAAG 
CAAGAACGGG AGAACGGCTC CAACCTGGCC TTCATGTTCC GCCTGCCATT TGCTGCTGGT 
CGAGTATTTA GTATCAGCAT GTTGGATACA CTGCTCTACC AGTCCTTCGT GAAGGACTAC 
ATGATCACCA TCACCAGGCT GCTCTTGGGC CTTGATACTA CACCAGGCTC CGGCTACCTC 
TGTGCAATGA AGGTAACCGA GGACGACCTG TGGATCCGCA CTTACGGCCG CCTCTTCCAG 
AAACTCTGCT CCTCCAGCGC CGAGATCCCC ATCGGCATCT ACAGGACCGA GTGCCATGTC 
TTCTCGGAGC CCCATGACGT CAGAGCCCAG GGGGGCCCAG ACATAGCCCT CCTGCCTGCC 
CAGTCTCAGA TCTCGGTGAA CATGGAGGAC TGCGAGGATA CTCGGGAGGC CAAGGGACCC 
TGGGGCACAC GAGCTGCATC TGGCAGTGGC AGCACCCATG GCCGTCACGG GGGCAGTGCT 
GACCCAGTGG AGCACCCACT ACTACGTCGC AAGAGCCTGC AGTGGGCCCG CAAGCTGAGT 
CGCAAGAGCA CCAAGCAGGC AGGGAAGGCA CCTGTGGCCA CAGACTGGAT CACCCAGCAG 
CGGCTCAGCC TGTACCGACG CTCAGAGCGC CAGGAGCTCT CAGAGCTGGT CAAGAACCGA 
ATGAAGCACC TGGGACTGCC CACCACTGGC TATGAGGACG TAGCAAATTT AACAGCCAGT 
GATGTGATGA ATCGGGTAAA CCTGGGATAT TTGCAAGATG AGATGAACGA TCATCACCAG 
AACACCCTTT CCTATGTACT CATCAACCCC CCGCCAGACA CAAGACTGGA ACCCAACGAC 
ATTGTGTACC TCATCCGTTC CGACCCCCTG GCCCATGTGG CCAGCAGCTC CCAGAGTCGG 
AAAAGCAGCT GCAGCAACAA GCTCTCATCC TGTAATCCTG AGACCAGGGA TGAGACCCAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497894.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497957.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X22, mRNA.

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CloneID	OMu73357
Clone ID Related Accession (Same CDS sequence)	XM_006497894.4 , XM_006497894.3
Accession Version	XM_006497894.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497894.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497957.2
CDS	215..2758
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDK

Target ORF information:

RefSeq Version	XM_006497894.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497894.3

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497895.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497958.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OMu73358
Clone ID Related Accession (Same CDS sequence)	XM_006497895.3 , XM_006497895.4
Accession Version	XM_006497895.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497895.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGCTGG AAGCAGATTA GGACTATTGT GTCCCTCATC CAAACACCTG 
TGTGCTTCCA GATGGATCCC TTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497958.2
CDS	218..2584
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKAGSRLGLLCPSSKHLCASRWIPC

Target ORF information:

RefSeq Version	XM_006497895.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497895.3

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGCTGG AAGCAGATTA GGACTATTGT GTCCCTCATC CAAACACCTG 
TGTGCTTCCA GATGGATCCC TTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497896.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497959.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OMu29456
Clone ID Related Accession (Same CDS sequence)	XM_006497896.4 , XM_030249694.1 , XM_006497896.3
Accession Version	XM_006497896.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497896.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGAA GAGTGCAAGT ACGCCATGCT GGCCCTGAAC TGCATCTGCC CGGCCACCTC 
CACCCTCATC ACCCTGCTGG TGCACACGTC CCGTGGCCAG TGAGTGCCCT GTGCCAGACC 
GACTACCACG CCCTGGTCAT CCCGATCAAC CCCACAAGGG AAGGACAGGA GTCTCCCGAG 
CAGTGGCAGC GCACGTACGG GAGGTGCTCG GGCAACGAGG TGTACCACAT TCGCATGGGT 
GACAGTAAAT TCTTCCGGGA GTATGAGGGC AAGAGCTTCA CCTACGCAGC CTTCCACGCG 
CACAAGAAAT ATGGGGTGTG CCTCATCGGG CTGAAGCGTG AGGAGAACAA GAGTATCCTG 
CTGAACCCAG GACCACGGCA CATCCTGGCT GCCTCCGACA CCTGCTTCTA TATCAATATT 
ACCAAGGAGG AGAACTCAGC TTTCATCTTC AAACAGGAGG AGAAGCAGAA GAGACGGGGC 
CTTGCAGGGC AGGCACTATA TGAAGGGCCC TCCCGGCTCC CAGTGCATAG CATCATCGCC 
TCTATGGGGA CAGTGGCCAT GGACCTGCAG AACACAGATT GCCGGCCCTC CCAGGGTGGC 
AGTGGCGGGG ACGGCACAAA GCTGACTCTG CCCACCGAGA ACGGCTCTGG TAGTCGACGC 
CCCAGCATCG CACCCGTTCT GGAGTTGGCA GACAGCTCAG CCCTGTTGCC CTGCGACCTG 
CTGAGTGACC AGTCAGAGGA TGAGGTGACA CCCTCGGACG ACGAGGGGCT CTCTGTGGTT 
GAGTACGTGA AGGGCTATCC CCCCAACTCA CCCTACATTG GCAGCTCCCC GACTTTATGC 
CACCTCCTGC CTGTGAAAGC CCCCTTCTGC TGCCTGCGGT TGGACAAGGG CTGCAAACAC 
AACAGCTACG AGGATGCCAA GGCCTATGGG TTCAAGAACA AGCTGATTAT TGTCTCTGCT 
GAGACGGCAG GCAACGGGCT CTACAACTTC ATCGTGCCTC TGCGCGCCTA CTACCGGTCC 
CGCCGGGAGC TCAACCCTAT CGTGTTGCTG CTTGACAACA AGCCTGACCA CCACTTCCTG 
GAGGCCATCT GTTGCTTCCC CATGGTCTAC TACATGGAGG GATCCGTGGA CAACCTGGAC 
AGCTTACTGC AGTGTGGCAT CATCTATGCT GACAACCTGG TGGTGGTGGA CAAGGAGAGC 
ACTATGAGCG CTGAAGAGGA CTACATGGCA GATGCCAAGA CCATCGTCAA TGTGCAAACC 
ATGTTCCGGC TTTTCCCCAG TCTCAGCATC ACCACGGAGC TCACACACCC TTCCAACATG 
CGGTTCATGC AGTTCCGTGC CAAGGACAGC TACTCTCTGG CTCTTTCCAA ACTTGAAAAG 
CAAGAACGGG AGAACGGCTC CAACCTGGCC TTCATGTTCC GCCTGCCATT TGCTGCTGGT 
CGAGTATTTA GTATCAGCAT GTTGGATACA CTGCTCTACC AGTCCTTCGT GAAGGACTAC 
ATGATCACCA TCACCAGGCT GCTCTTGGGC CTTGATACTA CACCAGGCTC CGGCTACCTC 
TGTGCAATGA AGGTAACCGA GGACGACCTG TGGATCCGCA CTTACGGCCG CCTCTTCCAG 
AAACTCTGCT CCTCCAGCGC CGAGATCCCC ATCGGCATCT ACAGGACCGA GTGCCATGTC 
TTCTCGGAGC CCCATGACGT CAGAGCCCAG GGGGGCCCAG ACATAGCCCT CCTGCCTGCC 
CAGTCTCAGA TCTCGGTGAA CATGGAGGAC TGCGAGGATA CTCGGGAGGC CAAGGGACCC 
TGGGGCACAC GAGCTGCATC TGGCAGTGGC AGCACCCATG GCCGTCACGG GGGCAGTGCT 
GACCCAGTGG AGCACCCACT ACTACGTCGC AAGAGCCTGC AGTGGGCCCG CAAGCTGAGT 
CGCAAGAGCA CCAAGCAGGC AGGGAAGGCA CCTGTGGCCA CAGACTGGAT CACCCAGCAG 
CGGCTCAGCC TGTACCGACG CTCAGAGCGC CAGGAGCTCT CAGAGCTGGT CAAGAACCGA 
ATGAAGCACC TGGGACTGCC CACCACTGGC TATGAGGACG TAGCAAATTT AACAGCCAGT 
GATGTGATGA ATCGGGTAAA CCTGGGATAT TTGCAAGATG AGATGAACGA TCATCACCAG 
AACACCCTTT CCTATGTACT CATCAACCCC CCGCCAGACA CAAGACTGGA ACCCAACGAC 
ATTGTGTACC TCATCCGTTC CGACCCCCTG GCCCATGTGG CCAGCAGCTC CCAGAGTCGG 
AAAAGCAGCT GCAGCAACAA GCTCTCATCC TGTAATCCTG AGACCAGGGA TGAGACCCAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497959.1
CDS	603..2948
Translation	MRGRVQVRHAGPELHLPGHLHPHHPAGAHVPWPVSALCQTDYHALVIPINPTREGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497896.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497896.4

ORF Insert Sequence:

ATGCGAGGAA GAGTGCAAGT ACGCCATGCT GGCCCTGAAC TGCATCTGCC CGGCCACCTC 
CACCCTCATC ACCCTGCTGG TGCACACGTC CCGTGGCCAG TGAGTGCCCT GTGCCAGACC 
GACTACCACG CCCTGGTCAT CCCGATCAAC CCCACAAGGG AAGGACAGGA GTCTCCCGAG 
CAGTGGCAGC GCACGTACGG GAGGTGCTCG GGCAACGAGG TGTACCACAT TCGCATGGGT 
GACAGTAAAT TCTTCCGGGA GTATGAGGGC AAGAGCTTCA CCTACGCAGC CTTCCACGCG 
CACAAGAAAT ATGGGGTGTG CCTCATCGGG CTGAAGCGTG AGGAGAACAA GAGTATCCTG 
CTGAACCCAG GACCACGGCA CATCCTGGCT GCCTCCGACA CCTGCTTCTA TATCAATATT 
ACCAAGGAGG AGAACTCAGC TTTCATCTTC AAACAGGAGG AGAAGCAGAA GAGACGGGGC 
CTTGCAGGGC AGGCACTATA TGAAGGGCCC TCCCGGCTCC CAGTGCATAG CATCATCGCC 
TCTATGGGGA CAGTGGCCAT GGACCTGCAG AACACAGATT GCCGGCCCTC CCAGGGTGGC 
AGTGGCGGGG ACGGCACAAA GCTGACTCTG CCCACCGAGA ACGGCTCTGG TAGTCGACGC 
CCCAGCATCG CACCCGTTCT GGAGTTGGCA GACAGCTCAG CCCTGTTGCC CTGCGACCTG 
CTGAGTGACC AGTCAGAGGA TGAGGTGACA CCCTCGGACG ACGAGGGGCT CTCTGTGGTT 
GAGTACGTGA AGGGCTATCC CCCCAACTCA CCCTACATTG GCAGCTCCCC GACTTTATGC 
CACCTCCTGC CTGTGAAAGC CCCCTTCTGC TGCCTGCGGT TGGACAAGGG CTGCAAACAC 
AACAGCTACG AGGATGCCAA GGCCTATGGG TTCAAGAACA AGCTGATTAT TGTCTCTGCT 
GAGACGGCAG GCAACGGGCT CTACAACTTC ATCGTGCCTC TGCGCGCCTA CTACCGGTCC 
CGCCGGGAGC TCAACCCTAT CGTGTTGCTG CTTGACAACA AGCCTGACCA CCACTTCCTG 
GAGGCCATCT GTTGCTTCCC CATGGTCTAC TACATGGAGG GATCCGTGGA CAACCTGGAC 
AGCTTACTGC AGTGTGGCAT CATCTATGCT GACAACCTGG TGGTGGTGGA CAAGGAGAGC 
ACTATGAGCG CTGAAGAGGA CTACATGGCA GATGCCAAGA CCATCGTCAA TGTGCAAACC 
ATGTTCCGGC TTTTCCCCAG TCTCAGCATC ACCACGGAGC TCACACACCC TTCCAACATG 
CGGTTCATGC AGTTCCGTGC CAAGGACAGC TACTCTCTGG CTCTTTCCAA ACTTGAAAAG 
CAAGAACGGG AGAACGGCTC CAACCTGGCC TTCATGTTCC GCCTGCCATT TGCTGCTGGT 
CGAGTATTTA GTATCAGCAT GTTGGATACA CTGCTCTACC AGTCCTTCGT GAAGGACTAC 
ATGATCACCA TCACCAGGCT GCTCTTGGGC CTTGATACTA CACCAGGCTC CGGCTACCTC 
TGTGCAATGA AGGTAACCGA GGACGACCTG TGGATCCGCA CTTACGGCCG CCTCTTCCAG 
AAACTCTGCT CCTCCAGCGC CGAGATCCCC ATCGGCATCT ACAGGACCGA GTGCCATGTC 
TTCTCGGAGC CCCATGACGT CAGAGCCCAG GGGGGCCCAG ACATAGCCCT CCTGCCTGCC 
CAGTCTCAGA TCTCGGTGAA CATGGAGGAC TGCGAGGATA CTCGGGAGGC CAAGGGACCC 
TGGGGCACAC GAGCTGCATC TGGCAGTGGC AGCACCCATG GCCGTCACGG GGGCAGTGCT 
GACCCAGTGG AGCACCCACT ACTACGTCGC AAGAGCCTGC AGTGGGCCCG CAAGCTGAGT 
CGCAAGAGCA CCAAGCAGGC AGGGAAGGCA CCTGTGGCCA CAGACTGGAT CACCCAGCAG 
CGGCTCAGCC TGTACCGACG CTCAGAGCGC CAGGAGCTCT CAGAGCTGGT CAAGAACCGA 
ATGAAGCACC TGGGACTGCC CACCACTGGC TATGAGGACG TAGCAAATTT AACAGCCAGT 
GATGTGATGA ATCGGGTAAA CCTGGGATAT TTGCAAGATG AGATGAACGA TCATCACCAG 
AACACCCTTT CCTATGTACT CATCAACCCC CCGCCAGACA CAAGACTGGA ACCCAACGAC 
ATTGTGTACC TCATCCGTTC CGACCCCCTG GCCCATGTGG CCAGCAGCTC CCAGAGTCGG 
AAAAGCAGCT GCAGCAACAA GCTCTCATCC TGTAATCCTG AGACCAGGGA TGAGACCCAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_030249694.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105554.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OMu29456
Clone ID Related Accession (Same CDS sequence)	XM_006497896.4 , XM_030249694.1 , XM_006497896.3
Accession Version	XM_030249694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGAA GAGTGCAAGT ACGCCATGCT GGCCCTGAAC TGCATCTGCC CGGCCACCTC 
CACCCTCATC ACCCTGCTGG TGCACACGTC CCGTGGCCAG TGAGTGCCCT GTGCCAGACC 
GACTACCACG CCCTGGTCAT CCCGATCAAC CCCACAAGGG AAGGACAGGA GTCTCCCGAG 
CAGTGGCAGC GCACGTACGG GAGGTGCTCG GGCAACGAGG TGTACCACAT TCGCATGGGT 
GACAGTAAAT TCTTCCGGGA GTATGAGGGC AAGAGCTTCA CCTACGCAGC CTTCCACGCG 
CACAAGAAAT ATGGGGTGTG CCTCATCGGG CTGAAGCGTG AGGAGAACAA GAGTATCCTG 
CTGAACCCAG GACCACGGCA CATCCTGGCT GCCTCCGACA CCTGCTTCTA TATCAATATT 
ACCAAGGAGG AGAACTCAGC TTTCATCTTC AAACAGGAGG AGAAGCAGAA GAGACGGGGC 
CTTGCAGGGC AGGCACTATA TGAAGGGCCC TCCCGGCTCC CAGTGCATAG CATCATCGCC 
TCTATGGGGA CAGTGGCCAT GGACCTGCAG AACACAGATT GCCGGCCCTC CCAGGGTGGC 
AGTGGCGGGG ACGGCACAAA GCTGACTCTG CCCACCGAGA ACGGCTCTGG TAGTCGACGC 
CCCAGCATCG CACCCGTTCT GGAGTTGGCA GACAGCTCAG CCCTGTTGCC CTGCGACCTG 
CTGAGTGACC AGTCAGAGGA TGAGGTGACA CCCTCGGACG ACGAGGGGCT CTCTGTGGTT 
GAGTACGTGA AGGGCTATCC CCCCAACTCA CCCTACATTG GCAGCTCCCC GACTTTATGC 
CACCTCCTGC CTGTGAAAGC CCCCTTCTGC TGCCTGCGGT TGGACAAGGG CTGCAAACAC 
AACAGCTACG AGGATGCCAA GGCCTATGGG TTCAAGAACA AGCTGATTAT TGTCTCTGCT 
GAGACGGCAG GCAACGGGCT CTACAACTTC ATCGTGCCTC TGCGCGCCTA CTACCGGTCC 
CGCCGGGAGC TCAACCCTAT CGTGTTGCTG CTTGACAACA AGCCTGACCA CCACTTCCTG 
GAGGCCATCT GTTGCTTCCC CATGGTCTAC TACATGGAGG GATCCGTGGA CAACCTGGAC 
AGCTTACTGC AGTGTGGCAT CATCTATGCT GACAACCTGG TGGTGGTGGA CAAGGAGAGC 
ACTATGAGCG CTGAAGAGGA CTACATGGCA GATGCCAAGA CCATCGTCAA TGTGCAAACC 
ATGTTCCGGC TTTTCCCCAG TCTCAGCATC ACCACGGAGC TCACACACCC TTCCAACATG 
CGGTTCATGC AGTTCCGTGC CAAGGACAGC TACTCTCTGG CTCTTTCCAA ACTTGAAAAG 
CAAGAACGGG AGAACGGCTC CAACCTGGCC TTCATGTTCC GCCTGCCATT TGCTGCTGGT 
CGAGTATTTA GTATCAGCAT GTTGGATACA CTGCTCTACC AGTCCTTCGT GAAGGACTAC 
ATGATCACCA TCACCAGGCT GCTCTTGGGC CTTGATACTA CACCAGGCTC CGGCTACCTC 
TGTGCAATGA AGGTAACCGA GGACGACCTG TGGATCCGCA CTTACGGCCG CCTCTTCCAG 
AAACTCTGCT CCTCCAGCGC CGAGATCCCC ATCGGCATCT ACAGGACCGA GTGCCATGTC 
TTCTCGGAGC CCCATGACGT CAGAGCCCAG GGGGGCCCAG ACATAGCCCT CCTGCCTGCC 
CAGTCTCAGA TCTCGGTGAA CATGGAGGAC TGCGAGGATA CTCGGGAGGC CAAGGGACCC 
TGGGGCACAC GAGCTGCATC TGGCAGTGGC AGCACCCATG GCCGTCACGG GGGCAGTGCT 
GACCCAGTGG AGCACCCACT ACTACGTCGC AAGAGCCTGC AGTGGGCCCG CAAGCTGAGT 
CGCAAGAGCA CCAAGCAGGC AGGGAAGGCA CCTGTGGCCA CAGACTGGAT CACCCAGCAG 
CGGCTCAGCC TGTACCGACG CTCAGAGCGC CAGGAGCTCT CAGAGCTGGT CAAGAACCGA 
ATGAAGCACC TGGGACTGCC CACCACTGGC TATGAGGACG TAGCAAATTT AACAGCCAGT 
GATGTGATGA ATCGGGTAAA CCTGGGATAT TTGCAAGATG AGATGAACGA TCATCACCAG 
AACACCCTTT CCTATGTACT CATCAACCCC CCGCCAGACA CAAGACTGGA ACCCAACGAC 
ATTGTGTACC TCATCCGTTC CGACCCCCTG GCCCATGTGG CCAGCAGCTC CCAGAGTCGG 
AAAAGCAGCT GCAGCAACAA GCTCTCATCC TGTAATCCTG AGACCAGGGA TGAGACCCAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105554.1
CDS	3372..5717
Translation	MRGRVQVRHAGPELHLPGHLHPHHPAGAHVPWPVSALCQTDYHALVIPINPTREGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_030249694.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249694.1

ORF Insert Sequence:

ATGCGAGGAA GAGTGCAAGT ACGCCATGCT GGCCCTGAAC TGCATCTGCC CGGCCACCTC 
CACCCTCATC ACCCTGCTGG TGCACACGTC CCGTGGCCAG TGAGTGCCCT GTGCCAGACC 
GACTACCACG CCCTGGTCAT CCCGATCAAC CCCACAAGGG AAGGACAGGA GTCTCCCGAG 
CAGTGGCAGC GCACGTACGG GAGGTGCTCG GGCAACGAGG TGTACCACAT TCGCATGGGT 
GACAGTAAAT TCTTCCGGGA GTATGAGGGC AAGAGCTTCA CCTACGCAGC CTTCCACGCG 
CACAAGAAAT ATGGGGTGTG CCTCATCGGG CTGAAGCGTG AGGAGAACAA GAGTATCCTG 
CTGAACCCAG GACCACGGCA CATCCTGGCT GCCTCCGACA CCTGCTTCTA TATCAATATT 
ACCAAGGAGG AGAACTCAGC TTTCATCTTC AAACAGGAGG AGAAGCAGAA GAGACGGGGC 
CTTGCAGGGC AGGCACTATA TGAAGGGCCC TCCCGGCTCC CAGTGCATAG CATCATCGCC 
TCTATGGGGA CAGTGGCCAT GGACCTGCAG AACACAGATT GCCGGCCCTC CCAGGGTGGC 
AGTGGCGGGG ACGGCACAAA GCTGACTCTG CCCACCGAGA ACGGCTCTGG TAGTCGACGC 
CCCAGCATCG CACCCGTTCT GGAGTTGGCA GACAGCTCAG CCCTGTTGCC CTGCGACCTG 
CTGAGTGACC AGTCAGAGGA TGAGGTGACA CCCTCGGACG ACGAGGGGCT CTCTGTGGTT 
GAGTACGTGA AGGGCTATCC CCCCAACTCA CCCTACATTG GCAGCTCCCC GACTTTATGC 
CACCTCCTGC CTGTGAAAGC CCCCTTCTGC TGCCTGCGGT TGGACAAGGG CTGCAAACAC 
AACAGCTACG AGGATGCCAA GGCCTATGGG TTCAAGAACA AGCTGATTAT TGTCTCTGCT 
GAGACGGCAG GCAACGGGCT CTACAACTTC ATCGTGCCTC TGCGCGCCTA CTACCGGTCC 
CGCCGGGAGC TCAACCCTAT CGTGTTGCTG CTTGACAACA AGCCTGACCA CCACTTCCTG 
GAGGCCATCT GTTGCTTCCC CATGGTCTAC TACATGGAGG GATCCGTGGA CAACCTGGAC 
AGCTTACTGC AGTGTGGCAT CATCTATGCT GACAACCTGG TGGTGGTGGA CAAGGAGAGC 
ACTATGAGCG CTGAAGAGGA CTACATGGCA GATGCCAAGA CCATCGTCAA TGTGCAAACC 
ATGTTCCGGC TTTTCCCCAG TCTCAGCATC ACCACGGAGC TCACACACCC TTCCAACATG 
CGGTTCATGC AGTTCCGTGC CAAGGACAGC TACTCTCTGG CTCTTTCCAA ACTTGAAAAG 
CAAGAACGGG AGAACGGCTC CAACCTGGCC TTCATGTTCC GCCTGCCATT TGCTGCTGGT 
CGAGTATTTA GTATCAGCAT GTTGGATACA CTGCTCTACC AGTCCTTCGT GAAGGACTAC 
ATGATCACCA TCACCAGGCT GCTCTTGGGC CTTGATACTA CACCAGGCTC CGGCTACCTC 
TGTGCAATGA AGGTAACCGA GGACGACCTG TGGATCCGCA CTTACGGCCG CCTCTTCCAG 
AAACTCTGCT CCTCCAGCGC CGAGATCCCC ATCGGCATCT ACAGGACCGA GTGCCATGTC 
TTCTCGGAGC CCCATGACGT CAGAGCCCAG GGGGGCCCAG ACATAGCCCT CCTGCCTGCC 
CAGTCTCAGA TCTCGGTGAA CATGGAGGAC TGCGAGGATA CTCGGGAGGC CAAGGGACCC 
TGGGGCACAC GAGCTGCATC TGGCAGTGGC AGCACCCATG GCCGTCACGG GGGCAGTGCT 
GACCCAGTGG AGCACCCACT ACTACGTCGC AAGAGCCTGC AGTGGGCCCG CAAGCTGAGT 
CGCAAGAGCA CCAAGCAGGC AGGGAAGGCA CCTGTGGCCA CAGACTGGAT CACCCAGCAG 
CGGCTCAGCC TGTACCGACG CTCAGAGCGC CAGGAGCTCT CAGAGCTGGT CAAGAACCGA 
ATGAAGCACC TGGGACTGCC CACCACTGGC TATGAGGACG TAGCAAATTT AACAGCCAGT 
GATGTGATGA ATCGGGTAAA CCTGGGATAT TTGCAAGATG AGATGAACGA TCATCACCAG 
AACACCCTTT CCTATGTACT CATCAACCCC CCGCCAGACA CAAGACTGGA ACCCAACGAC 
ATTGTGTACC TCATCCGTTC CGACCCCCTG GCCCATGTGG CCAGCAGCTC CCAGAGTCGG 
AAAAGCAGCT GCAGCAACAA GCTCTCATCC TGTAATCCTG AGACCAGGGA TGAGACCCAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_030249677.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105537.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OMu73356
Clone ID Related Accession (Same CDS sequence)	XM_030249677.1
Accession Version	XM_030249677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGG TCAGCTGGAA TGTGGAGAAA GTCCTTCGTG AAGGACTACA TGATCACCAT 
CACCAGGCTG CTCTTGGGCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105537.1
CDS	206..3169
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQVSWNVEKVLREGLHDHHHQAALGP

Target ORF information:

RefSeq Version	XM_030249677.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249677.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGG TCAGCTGGAA TGTGGAGAAA GTCCTTCGTG AAGGACTACA TGATCACCAT 
CACCAGGCTG CTCTTGGGCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497894.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497957.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OMu73357
Clone ID Related Accession (Same CDS sequence)	XM_006497894.4 , XM_006497894.3
Accession Version	XM_006497894.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497894.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497957.2
CDS	211..2754
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDK

Target ORF information:

RefSeq Version	XM_006497894.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497894.4

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497895.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497958.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X24, mRNA.

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CloneID	OMu73358
Clone ID Related Accession (Same CDS sequence)	XM_006497895.3 , XM_006497895.4
Accession Version	XM_006497895.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497895.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGCTGG AAGCAGATTA GGACTATTGT GTCCCTCATC CAAACACCTG 
TGTGCTTCCA GATGGATCCC TTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497958.2
CDS	215..2581
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKAGSRLGLLCPSSKHLCASRWIPC

Target ORF information:

RefSeq Version	XM_006497895.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497895.4

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGCTGG AAGCAGATTA GGACTATTGT GTCCCTCATC CAAACACCTG 
TGTGCTTCCA GATGGATCCC TTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_017317204.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172693.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OMu80190
Clone ID Related Accession (Same CDS sequence)	XM_017317204.1
Accession Version	XM_017317204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAATG ACTTCCACCG TGCCATCCTA CGCACACAGT CAGCCATGTT CAACCAGGTG 
CTCATCCTGT TCTGCACCCT GCTGTGCCTG GTCTTCACAG GGACCTGTGG GATCCAGCAC 
CTGGAGCGAG CAGGTGGCAA CTTGAACCTG CTGACCTCCT TCTACTTCTG CATCGTGACT 
TTCTCAACTG TGGGCTTCGG TGATGTGACG CCCAAGATCT GGCCATCCCA GCTCCTGGTG 
GTCATCCTGA TCTGTGTCAC CCTTGTGGTG CTCCCACTGC AATTTGAAGA GCTTGTCTAC 
CTCTGGATGG AGCGTCAGAA GTCAGGGGGC AACTATAGCC GCCACCGAGC ACGGACGGAG 
AAGCACGTAG TCCTGTGTGT GAGCTCCCTC AAGATTGATC TCCTCATGGA CTTCCTGAAT 
GAGTTCTATG CCCATCCCCG GCTCCAGGAC TACTACGTGG TCATCCTGTG TCCCTCTGAA 
ATGGACGTCC AGGTGCGCAG GGTGCTGCAG ATTCCCCTGT GGTCCCAGCG GGTCATCTAC 
CTCCAGGGCT CTGCCCTCAA GGACCAGGAT CTCATGCGAG CCAAGATGGA CAACGGAGAG 
GCCTGCTTTA TCCTCAGCAG CAGGAATGAG GTGGACCGCA CAGCTGCGGA TCACCAGACC 
ATCCTTCGAG CCTGGGCTGT GAAGGACTTT GCCCCCAACT GTCCCCTCTA TGTCCAGATC 
CTCAAGCCCG AAAACAAGTT TCACGTCAAA TTTGCTGACC ACGTGGTATG CGAGGAAGAG 
TGCAAGTACG CCATGCTGGC CCTGAACTGC ATCTGCCCGG CCACCTCCAC CCTCATCACC 
CTGCTGGTGC ACACGTCCCG TGGCCAGGAA GGACAGGAGT CTCCCGAGCA GTGGCAGCGC 
ACGTACGGGA GGTGCTCGGG CAACGAGGTG TACCACATTC GCATGGGTGA CAGTAAATTC 
TTCCGGGAGT ATGAGGGCAA GAGCTTCACC TACGCAGCCT TCCACGCGCA CAAGAAATAT 
GGGGTGTGCC TCATCGGGCT GAAGCGTGAG GAGAACAAGA GTATCCTGCT GAACCCAGGA 
CCACGGCACA TCCTGGCTGC CTCCGACACC TGCTTCTATA TCAATATTAC CAAGGAGGAG 
AACTCAGCTT TCATCTTCAA ACAGGAGGAG AAGCAGAAGA GACGGGGCCT TGCAGGGCAG 
GCACTATATG AAGGGCCCTC CCGGCTCCCA GTGCATAGCA TCATCGCCTC TATGGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA 
AAGCTGACTC TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GATGAGGTGA CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT 
CCCCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC 
AAGGCCTATG GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT 
ATCGTGTTGC TGCTTGACAA CAACCTGGAC AGCTTACTGC AGTGTGGCAT CATCTATGCT 
GACAACCTGG TGGTGGTGGA CAAGGAGAGC ACTATGAGCG CTGAAGAGGA CTACATGGCA 
GATGCCAAGA CCATCGTCAA TGTGCAAACC ATGTTCCGGC TTTTCCCCAG TCTCAGCATC 
ACCACGGAGC TCACACACCC TTCCAACATG CGGTTCATGC AGTTCCGTGC CAAGGACAGC 
TACTCTCTGG CTCTTTCCAA ACTTGAAAAG CAAGAACGGG AGAACGGCTC CAACCTGGCC 
TTCATGTTCC GCCTGCCATT TGCTGCTGGT CGAGTATTTA GTATCAGCAT GTTGGATACA 
CTGCTCTACC AGTCCTTCGT GAAGGACTAC ATGATCACCA TCACCAGGCT GCTCTTGGGC 
CTTGATACTA CACCAGGCTC CGGCTACCTC TGTGCAATGA AGGTAACCGA GGACGACCTG 
TGGATCCGCA CTTACGGCCG CCTCTTCCAG AAACTCTGCT CCTCCAGCGC CGAGATCCCC 
ATCGGCATCT ACAGGACCGA GTGCCATGTC TTCTCGGAGC CCCATGACGT CAGAGCCCAG 
TCTCAGATCT CGGTGAACAT GGAGGACTGC GAGGATACTC GGGAGGCCAA GGGACCCTGG 
GGCACACGAG CTGCATCTGG CAGTGGCAGC ACCCATGGCC GTCACGGGGG CAGTGCTGAC 
CCAGTGGAGC ACCCACTACT ACGTCGCAAG AGCCTGCAGT GGGCCCGCAA GCTGAGTCGC 
AAGAGCACCA AGCAGGCAGG GAAGGCACCT GTGGCCACAG ACTGGATCAC CCAGCAGCGG 
CTCAGCCTGT ACCGACGCTC AGAGCGCCAG GAGCTCTCAG AGCTGGTCAA GAACCGAATG 
AAGCACCTGG GACTGCCCAC CACTGGCTAT GATGAGATGA ACGATCATCA CCAGAACACC 
CTTTCCTATG TACTCATCAA CCCCCCGCCA GACACAAGAC TGGAACCCAA CGACATTGTG 
TACCTCATCC GTTCCGACCC CCTGGCCCAT GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC 
AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172693.1
CDS	2719..5598
Translation	MINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_017317204.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317204.1

ORF Insert Sequence:

ATGATTAATG ACTTCCACCG TGCCATCCTA CGCACACAGT CAGCCATGTT CAACCAGGTG 
CTCATCCTGT TCTGCACCCT GCTGTGCCTG GTCTTCACAG GGACCTGTGG GATCCAGCAC 
CTGGAGCGAG CAGGTGGCAA CTTGAACCTG CTGACCTCCT TCTACTTCTG CATCGTGACT 
TTCTCAACTG TGGGCTTCGG TGATGTGACG CCCAAGATCT GGCCATCCCA GCTCCTGGTG 
GTCATCCTGA TCTGTGTCAC CCTTGTGGTG CTCCCACTGC AATTTGAAGA GCTTGTCTAC 
CTCTGGATGG AGCGTCAGAA GTCAGGGGGC AACTATAGCC GCCACCGAGC ACGGACGGAG 
AAGCACGTAG TCCTGTGTGT GAGCTCCCTC AAGATTGATC TCCTCATGGA CTTCCTGAAT 
GAGTTCTATG CCCATCCCCG GCTCCAGGAC TACTACGTGG TCATCCTGTG TCCCTCTGAA 
ATGGACGTCC AGGTGCGCAG GGTGCTGCAG ATTCCCCTGT GGTCCCAGCG GGTCATCTAC 
CTCCAGGGCT CTGCCCTCAA GGACCAGGAT CTCATGCGAG CCAAGATGGA CAACGGAGAG 
GCCTGCTTTA TCCTCAGCAG CAGGAATGAG GTGGACCGCA CAGCTGCGGA TCACCAGACC 
ATCCTTCGAG CCTGGGCTGT GAAGGACTTT GCCCCCAACT GTCCCCTCTA TGTCCAGATC 
CTCAAGCCCG AAAACAAGTT TCACGTCAAA TTTGCTGACC ACGTGGTATG CGAGGAAGAG 
TGCAAGTACG CCATGCTGGC CCTGAACTGC ATCTGCCCGG CCACCTCCAC CCTCATCACC 
CTGCTGGTGC ACACGTCCCG TGGCCAGGAA GGACAGGAGT CTCCCGAGCA GTGGCAGCGC 
ACGTACGGGA GGTGCTCGGG CAACGAGGTG TACCACATTC GCATGGGTGA CAGTAAATTC 
TTCCGGGAGT ATGAGGGCAA GAGCTTCACC TACGCAGCCT TCCACGCGCA CAAGAAATAT 
GGGGTGTGCC TCATCGGGCT GAAGCGTGAG GAGAACAAGA GTATCCTGCT GAACCCAGGA 
CCACGGCACA TCCTGGCTGC CTCCGACACC TGCTTCTATA TCAATATTAC CAAGGAGGAG 
AACTCAGCTT TCATCTTCAA ACAGGAGGAG AAGCAGAAGA GACGGGGCCT TGCAGGGCAG 
GCACTATATG AAGGGCCCTC CCGGCTCCCA GTGCATAGCA TCATCGCCTC TATGGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA 
AAGCTGACTC TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GATGAGGTGA CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT 
CCCCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC 
AAGGCCTATG GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT 
ATCGTGTTGC TGCTTGACAA CAACCTGGAC AGCTTACTGC AGTGTGGCAT CATCTATGCT 
GACAACCTGG TGGTGGTGGA CAAGGAGAGC ACTATGAGCG CTGAAGAGGA CTACATGGCA 
GATGCCAAGA CCATCGTCAA TGTGCAAACC ATGTTCCGGC TTTTCCCCAG TCTCAGCATC 
ACCACGGAGC TCACACACCC TTCCAACATG CGGTTCATGC AGTTCCGTGC CAAGGACAGC 
TACTCTCTGG CTCTTTCCAA ACTTGAAAAG CAAGAACGGG AGAACGGCTC CAACCTGGCC 
TTCATGTTCC GCCTGCCATT TGCTGCTGGT CGAGTATTTA GTATCAGCAT GTTGGATACA 
CTGCTCTACC AGTCCTTCGT GAAGGACTAC ATGATCACCA TCACCAGGCT GCTCTTGGGC 
CTTGATACTA CACCAGGCTC CGGCTACCTC TGTGCAATGA AGGTAACCGA GGACGACCTG 
TGGATCCGCA CTTACGGCCG CCTCTTCCAG AAACTCTGCT CCTCCAGCGC CGAGATCCCC 
ATCGGCATCT ACAGGACCGA GTGCCATGTC TTCTCGGAGC CCCATGACGT CAGAGCCCAG 
TCTCAGATCT CGGTGAACAT GGAGGACTGC GAGGATACTC GGGAGGCCAA GGGACCCTGG 
GGCACACGAG CTGCATCTGG CAGTGGCAGC ACCCATGGCC GTCACGGGGG CAGTGCTGAC 
CCAGTGGAGC ACCCACTACT ACGTCGCAAG AGCCTGCAGT GGGCCCGCAA GCTGAGTCGC 
AAGAGCACCA AGCAGGCAGG GAAGGCACCT GTGGCCACAG ACTGGATCAC CCAGCAGCGG 
CTCAGCCTGT ACCGACGCTC AGAGCGCCAG GAGCTCTCAG AGCTGGTCAA GAACCGAATG 
AAGCACCTGG GACTGCCCAC CACTGGCTAT GATGAGATGA ACGATCATCA CCAGAACACC 
CTTTCCTATG TACTCATCAA CCCCCCGCCA GACACAAGAC TGGAACCCAA CGACATTGTG 
TACCTCATCC GTTCCGACCC CCTGGCCCAT GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC 
AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_017317206.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172695.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OMu80191
Clone ID Related Accession (Same CDS sequence)	XM_017317206.1
Accession Version	XM_017317206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACG GAGAGGCCTG CTTTATCCTC AGCAGCAGGA ATGAGGTGGA CCGCACAGCT 
GCGGATCACC AGACCATCCT TCGAGCCTGG GCTGTGAAGG ACTTTGCCCC CAACTGTCCC 
CTCTATGTCC AGATCCTCAA GCCCGAAAAC AAGTTTCACG TCAAATTTGC TGACCACGTG 
GTATGCGAGG AAGAGTGCAA GTACGCCATG CTGGCCCTGA ACTGCATCTG CCCGGCCACC 
TCCACCCTCA TCACCCTGCT GGTGCACACG TCCCGTGGCC AGGAAGGACA GGAGTCTCCC 
GAGCAGTGGC AGCGCACGTA CGGGAGGTGC TCGGGCAACG AGGTGTACCA CATTCGCATG 
GGTGACAGTA AATTCTTCCG GGAGTATGAG GGCAAGAGCT TCACCTACGC AGCCTTCCAC 
GCGCACAAGA AATATGGGGT GTGCCTCATC GGGCTGAAGC GTGAGGAGAA CAAGAGTATC 
CTGCTGAACC CAGGACCACG GCACATCCTG GCTGCCTCCG ACACCTGCTT CTATATCAAT 
ATTACCAAGG AGGAGAACTC AGCTTTCATC TTCAAACAGG AGGAGAAGCA GAAGAGACGG 
GGCCTTGCAG GGCAGGCACT ATATGAAGGG CCCTCCCGGC TCCCAGTGCA TAGCATCATC 
GCCTCTATGG GGACAGTGGC CATGGACCTG CAGAACACAG ATTGCCGGCC CTCCCAGGGT 
GGCAGTGGCG GGGACGGCAC AAAGCTGACT CTGCCCACCG AGAACGGCTC TGGTAGTCGA 
CGCCCCAGCA TCGCACCCGT TCTGGAGTTG GCAGACAGCT CAGCCCTGTT GCCCTGCGAC 
CTGCTGAGTG ACCAGTCAGA GGATGAGGTG ACACCCTCGG ACGACGAGGG GCTCTCTGTG 
GTTGAGTACG TGAAGGGCTA TCCCCCCAAC TCACCCTACA TTGGCAGCTC CCCGACTTTA 
TGCCACCTCC TGCCTGTGAA AGCCCCCTTC TGCTGCCTGC GGTTGGACAA GGGCTGCAAA 
CACAACAGCT ACGAGGATGC CAAGGCCTAT GGGTTCAAGA ACAAGCTGAT TATTGTCTCT 
GCTGAGACGG CAGGCAACGG GCTCTACAAC TTCATCGTGC CTCTGCGCGC CTACTACCGG 
TCCCGCCGGG AGCTCAACCC TATCGTGTTG CTGCTTGACA ACAAGCCTGA CCACCACTTC 
CTGGAGGCCA TCTGTTGCTT CCCCATGGTC TACTACATGG AGGGATCCGT GGACAACCTG 
GACAGCTTAC TGCAGTGTGG CATCATCTAT GCTGACAACC TGGTGGTGGT GGACAAGGAG 
AGCACTATGA GCGCTGAAGA GGACTACATG GCAGATGCCA AGACCATCGT CAATGTGCAA 
ACCATGTTCC GGCTTTTCCC CAGTCTCAGC ATCACCACGG AGCTCACACA CCCTTCCAAC 
ATGCGGTTCA TGCAGTTCCG TGCCAAGGAC AGCTACTCTC TGGCTCTTTC CAAACTTGAA 
AAGCAAGAAC GGGAGAACGG CTCCAACCTG GCCTTCATGT TCCGCCTGCC ATTTGCTGCT 
GGTCGAGTAT TTAGTATCAG CATGTTGGAT ACACTGCTCT ACCAGTCCTT CGTGAAGGAC 
TACATGATCA CCATCACCAG GCTGCTCTTG GGCCTTGATA CTACACCAGG CTCCGGCTAC 
CTCTGTGCAA TGAAGGTAAC CGAGGACGAC CTGTGGATCC GCACTTACGG CCGCCTCTTC 
CAGAAACTCT GCTCCTCCAG CGCCGAGATC CCCATCGGCA TCTACAGGAC CGAGTGCCAT 
GTCTTCTCGG AGCCCCATGA CGTCAGAGCC CAGTCTCAGA TCTCGGTGAA CATGGAGGAC 
TGCGAGGATA CTCGGGAGGC CAAGGGACCC TGGGGCACAC GAGCTGCATC TGGCAGTGGC 
AGCACCCATG GCCGTCACGG GGGCAGTGCT GACCCAGTGG AGCACCCACT ACTACGTCGC 
AAGAGCCTGC AGTGGGCCCG CAAGCTGAGT CGCAAGAGCA CCAAGCAGGC AGGGAAGGCA 
CCTGTGGCCA CAGACTGGAT CACCCAGCAG CGGCTCAGCC TGTACCGACG CTCAGAGCGC 
CAGGAGCTCT CAGAGCTGGT CAAGAACCGA ATGAAGCACC TGGGACTGCC CACCACTGGC 
TATGAGGACG TAGCAAATTT AACAGCCAGT GATGTGATGA ATCGGGTAAA CCTGGGATAT 
TTGCAAGATG AGATGAACGA TCATCACCAG AACACCCTTT CCTATGTACT CATCAACCCC 
CCGCCAGACA CAAGACTGGA ACCCAACGAC ATTGTGTACC TCATCCGTTC CGACCCCCTG 
GCCCATGTGG CCAGCAGCTC CCAGAGTCGG AAAAGCAGCT GCAGCAACAA GCTCTCATCC 
TGTAATCCTG AGACCAGGGA TGAGACCCAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172695.1
CDS	3304..5739
Translation	MDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_017317206.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317206.1

ORF Insert Sequence:

ATGGACAACG GAGAGGCCTG CTTTATCCTC AGCAGCAGGA ATGAGGTGGA CCGCACAGCT 
GCGGATCACC AGACCATCCT TCGAGCCTGG GCTGTGAAGG ACTTTGCCCC CAACTGTCCC 
CTCTATGTCC AGATCCTCAA GCCCGAAAAC AAGTTTCACG TCAAATTTGC TGACCACGTG 
GTATGCGAGG AAGAGTGCAA GTACGCCATG CTGGCCCTGA ACTGCATCTG CCCGGCCACC 
TCCACCCTCA TCACCCTGCT GGTGCACACG TCCCGTGGCC AGGAAGGACA GGAGTCTCCC 
GAGCAGTGGC AGCGCACGTA CGGGAGGTGC TCGGGCAACG AGGTGTACCA CATTCGCATG 
GGTGACAGTA AATTCTTCCG GGAGTATGAG GGCAAGAGCT TCACCTACGC AGCCTTCCAC 
GCGCACAAGA AATATGGGGT GTGCCTCATC GGGCTGAAGC GTGAGGAGAA CAAGAGTATC 
CTGCTGAACC CAGGACCACG GCACATCCTG GCTGCCTCCG ACACCTGCTT CTATATCAAT 
ATTACCAAGG AGGAGAACTC AGCTTTCATC TTCAAACAGG AGGAGAAGCA GAAGAGACGG 
GGCCTTGCAG GGCAGGCACT ATATGAAGGG CCCTCCCGGC TCCCAGTGCA TAGCATCATC 
GCCTCTATGG GGACAGTGGC CATGGACCTG CAGAACACAG ATTGCCGGCC CTCCCAGGGT 
GGCAGTGGCG GGGACGGCAC AAAGCTGACT CTGCCCACCG AGAACGGCTC TGGTAGTCGA 
CGCCCCAGCA TCGCACCCGT TCTGGAGTTG GCAGACAGCT CAGCCCTGTT GCCCTGCGAC 
CTGCTGAGTG ACCAGTCAGA GGATGAGGTG ACACCCTCGG ACGACGAGGG GCTCTCTGTG 
GTTGAGTACG TGAAGGGCTA TCCCCCCAAC TCACCCTACA TTGGCAGCTC CCCGACTTTA 
TGCCACCTCC TGCCTGTGAA AGCCCCCTTC TGCTGCCTGC GGTTGGACAA GGGCTGCAAA 
CACAACAGCT ACGAGGATGC CAAGGCCTAT GGGTTCAAGA ACAAGCTGAT TATTGTCTCT 
GCTGAGACGG CAGGCAACGG GCTCTACAAC TTCATCGTGC CTCTGCGCGC CTACTACCGG 
TCCCGCCGGG AGCTCAACCC TATCGTGTTG CTGCTTGACA ACAAGCCTGA CCACCACTTC 
CTGGAGGCCA TCTGTTGCTT CCCCATGGTC TACTACATGG AGGGATCCGT GGACAACCTG 
GACAGCTTAC TGCAGTGTGG CATCATCTAT GCTGACAACC TGGTGGTGGT GGACAAGGAG 
AGCACTATGA GCGCTGAAGA GGACTACATG GCAGATGCCA AGACCATCGT CAATGTGCAA 
ACCATGTTCC GGCTTTTCCC CAGTCTCAGC ATCACCACGG AGCTCACACA CCCTTCCAAC 
ATGCGGTTCA TGCAGTTCCG TGCCAAGGAC AGCTACTCTC TGGCTCTTTC CAAACTTGAA 
AAGCAAGAAC GGGAGAACGG CTCCAACCTG GCCTTCATGT TCCGCCTGCC ATTTGCTGCT 
GGTCGAGTAT TTAGTATCAG CATGTTGGAT ACACTGCTCT ACCAGTCCTT CGTGAAGGAC 
TACATGATCA CCATCACCAG GCTGCTCTTG GGCCTTGATA CTACACCAGG CTCCGGCTAC 
CTCTGTGCAA TGAAGGTAAC CGAGGACGAC CTGTGGATCC GCACTTACGG CCGCCTCTTC 
CAGAAACTCT GCTCCTCCAG CGCCGAGATC CCCATCGGCA TCTACAGGAC CGAGTGCCAT 
GTCTTCTCGG AGCCCCATGA CGTCAGAGCC CAGTCTCAGA TCTCGGTGAA CATGGAGGAC 
TGCGAGGATA CTCGGGAGGC CAAGGGACCC TGGGGCACAC GAGCTGCATC TGGCAGTGGC 
AGCACCCATG GCCGTCACGG GGGCAGTGCT GACCCAGTGG AGCACCCACT ACTACGTCGC 
AAGAGCCTGC AGTGGGCCCG CAAGCTGAGT CGCAAGAGCA CCAAGCAGGC AGGGAAGGCA 
CCTGTGGCCA CAGACTGGAT CACCCAGCAG CGGCTCAGCC TGTACCGACG CTCAGAGCGC 
CAGGAGCTCT CAGAGCTGGT CAAGAACCGA ATGAAGCACC TGGGACTGCC CACCACTGGC 
TATGAGGACG TAGCAAATTT AACAGCCAGT GATGTGATGA ATCGGGTAAA CCTGGGATAT 
TTGCAAGATG AGATGAACGA TCATCACCAG AACACCCTTT CCTATGTACT CATCAACCCC 
CCGCCAGACA CAAGACTGGA ACCCAACGAC ATTGTGTACC TCATCCGTTC CGACCCCCTG 
GCCCATGTGG CCAGCAGCTC CCAGAGTCGG AAAAGCAGCT GCAGCAACAA GCTCTCATCC 
TGTAATCCTG AGACCAGGGA TGAGACCCAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_017317207.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172696.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OMu80192
Clone ID Related Accession (Same CDS sequence)	XM_017317207.1
Accession Version	XM_017317207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGAA GAGTGCAAGT ACGCCATGCT GGCCCTGAAC TGCATCTGCC CGGCCACCTC 
CACCCTCATC ACCCTGCTGG TGCACACGTC CCGTGGCCAG TGAGTGCCCT GTGCCAGACC 
GACTACCACG CCCTGGTCAT CCCGATCAAC CCCACAAGGG AAGGACAGGA GTCTCCCGAG 
CAGTGGCAGC GCACGTACGG GAGGTGCTCG GGCAACGAGG TGTACCACAT TCGCATGGGT 
GACAGTAAAT TCTTCCGGGA GTATGAGGGC AAGAGCTTCA CCTACGCAGC CTTCCACGCG 
CACAAGAAAT ATGGGGTGTG CCTCATCGGG CTGAAGCGTG AGGAGAACAA GAGTATCCTG 
CTGAACCCAG GACCACGGCA CATCCTGGCT GCCTCCGACA CCTGCTTCTA TATCAATATT 
ACCAAGGAGG AGAACTCAGC TTTCATCTTC AAACAGGAGG AGAAGCAGAA GAGACGGGGC 
CTTGCAGGGC AGGCACTATA TGAAGGGCCC TCCCGGCTCC CAGTGCATAG CATCATCGCC 
TCTATGGGGA CAGTGGCCAT GGACCTGCAG AACACAGATT GCCGGCCCTC CCAGGGTGGC 
AGTGGCGGGG ACGGCACAAA GCTGACTCTG CCCACCGAGA ACGGCTCTGG TAGTCGACGC 
CCCAGCATCG CACCCGTTCT GGAGTTGGCA GACAGCTCAG CCCTGTTGCC CTGCGACCTG 
CTGAGTGACC AGTCAGAGGA TGAGGTGACA CCCTCGGACG ACGAGGGGCT CTCTGTGGTT 
GAGTACGTGA AGGGCTATCC CCCCAACTCA CCCTACATTG GCAGCTCCCC GACTTTATGC 
CACCTCCTGC CTGTGAAAGC CCCCTTCTGC TGCCTGCGGT TGGACAAGGG CTGCAAACAC 
AACAGCTACG AGGATGCCAA GGCCTATGGG TTCAAGAACA AGCTGATTAT TGTCTCTGCT 
GAGACGGCAG GCAACGGGCT CTACAACTTC ATCGTGCCTC TGCGCGCCTA CTACCGGTCC 
CGCCGGGAGC TCAACCCTAT CGTGTTGCTG CTTGACAACA AGCCTGACCA CCACTTCCTG 
GAGGCCATCT GTTGCTTCCC CATGGTCTAC TACATGGAGG GATCCGTGGA CAACCTGGAC 
AGCTTACTGC AGTGTGGCAT CATCTATGCT GACAACCTGG TGGTGGTGGA CAAGGAGAGC 
ACTATGAGCG CTGAAGAGGA CTACATGGCA GATGCCAAGA CCATCGTCAA TGTGCAAACC 
ATGTTCCGGC TTTTCCCCAG TCTCAGCATC ACCACGGAGC TCACACACCC TTCCAACATG 
CGGTTCATGC AGTTCCGTGC CAAGGACAGC TACTCTCTGG CTCTTTCCAA ACTTGAAAAG 
CAAGAACGGG AGAACGGCTC CAACCTGGCC TTCATGTTCC GCCTGCCATT TGCTGCTGGT 
CGAGTATTTA GTATCAGCAT GTTGGATACA CTGCTCTACC AGTCCTTCGT GAAGGACTAC 
ATGATCACCA TCACCAGGCT GCTCTTGGGC CTTGATACTA CACCAGGCTC CGGCTACCTC 
TGTGCAATGA AGGTAACCGA GGACGACCTG TGGATCCGCA CTTACGGCCG CCTCTTCCAG 
AAACTCTGCT CCTCCAGCGC CGAGATCCCC ATCGGCATCT ACAGGACCGA GTGCCATGTC 
TTCTCGGAGC CCCATGACGT CAGAGCCCAG TCTCAGATCT CGGTGAACAT GGAGGACTGC 
GAGGATACTC GGGAGGCCAA GGGACCCTGG GGCACACGAG CTGCATCTGG CAGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCAGTGGAGC ACCCACTACT ACGTCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGTCGC AAGAGCACCA AGCAGGCAGG GAAGGCACCT 
GTGGCCACAG ACTGGATCAC CCAGCAGCGG CTCAGCCTGT ACCGACGCTC AGAGCGCCAG 
GAGCTCTCAG AGCTGGTCAA GAACCGAATG AAGCACCTGG GACTGCCCAC CACTGGCTAT 
GATGAGATGA ACGATCATCA CCAGAACACC CTTTCCTATG TACTCATCAA CCCCCCGCCA 
GACACAAGAC TGGAACCCAA CGACATTGTG TACCTCATCC GTTCCGACCC CCTGGCCCAT 
GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT 
CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172696.1
CDS	888..3137
Translation	MRGRVQVRHAGPELHLPGHLHPHHPAGAHVPWPVSALCQTDYHALVIPINPTREGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_017317207.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317207.1

ORF Insert Sequence:

ATGCGAGGAA GAGTGCAAGT ACGCCATGCT GGCCCTGAAC TGCATCTGCC CGGCCACCTC 
CACCCTCATC ACCCTGCTGG TGCACACGTC CCGTGGCCAG TGAGTGCCCT GTGCCAGACC 
GACTACCACG CCCTGGTCAT CCCGATCAAC CCCACAAGGG AAGGACAGGA GTCTCCCGAG 
CAGTGGCAGC GCACGTACGG GAGGTGCTCG GGCAACGAGG TGTACCACAT TCGCATGGGT 
GACAGTAAAT TCTTCCGGGA GTATGAGGGC AAGAGCTTCA CCTACGCAGC CTTCCACGCG 
CACAAGAAAT ATGGGGTGTG CCTCATCGGG CTGAAGCGTG AGGAGAACAA GAGTATCCTG 
CTGAACCCAG GACCACGGCA CATCCTGGCT GCCTCCGACA CCTGCTTCTA TATCAATATT 
ACCAAGGAGG AGAACTCAGC TTTCATCTTC AAACAGGAGG AGAAGCAGAA GAGACGGGGC 
CTTGCAGGGC AGGCACTATA TGAAGGGCCC TCCCGGCTCC CAGTGCATAG CATCATCGCC 
TCTATGGGGA CAGTGGCCAT GGACCTGCAG AACACAGATT GCCGGCCCTC CCAGGGTGGC 
AGTGGCGGGG ACGGCACAAA GCTGACTCTG CCCACCGAGA ACGGCTCTGG TAGTCGACGC 
CCCAGCATCG CACCCGTTCT GGAGTTGGCA GACAGCTCAG CCCTGTTGCC CTGCGACCTG 
CTGAGTGACC AGTCAGAGGA TGAGGTGACA CCCTCGGACG ACGAGGGGCT CTCTGTGGTT 
GAGTACGTGA AGGGCTATCC CCCCAACTCA CCCTACATTG GCAGCTCCCC GACTTTATGC 
CACCTCCTGC CTGTGAAAGC CCCCTTCTGC TGCCTGCGGT TGGACAAGGG CTGCAAACAC 
AACAGCTACG AGGATGCCAA GGCCTATGGG TTCAAGAACA AGCTGATTAT TGTCTCTGCT 
GAGACGGCAG GCAACGGGCT CTACAACTTC ATCGTGCCTC TGCGCGCCTA CTACCGGTCC 
CGCCGGGAGC TCAACCCTAT CGTGTTGCTG CTTGACAACA AGCCTGACCA CCACTTCCTG 
GAGGCCATCT GTTGCTTCCC CATGGTCTAC TACATGGAGG GATCCGTGGA CAACCTGGAC 
AGCTTACTGC AGTGTGGCAT CATCTATGCT GACAACCTGG TGGTGGTGGA CAAGGAGAGC 
ACTATGAGCG CTGAAGAGGA CTACATGGCA GATGCCAAGA CCATCGTCAA TGTGCAAACC 
ATGTTCCGGC TTTTCCCCAG TCTCAGCATC ACCACGGAGC TCACACACCC TTCCAACATG 
CGGTTCATGC AGTTCCGTGC CAAGGACAGC TACTCTCTGG CTCTTTCCAA ACTTGAAAAG 
CAAGAACGGG AGAACGGCTC CAACCTGGCC TTCATGTTCC GCCTGCCATT TGCTGCTGGT 
CGAGTATTTA GTATCAGCAT GTTGGATACA CTGCTCTACC AGTCCTTCGT GAAGGACTAC 
ATGATCACCA TCACCAGGCT GCTCTTGGGC CTTGATACTA CACCAGGCTC CGGCTACCTC 
TGTGCAATGA AGGTAACCGA GGACGACCTG TGGATCCGCA CTTACGGCCG CCTCTTCCAG 
AAACTCTGCT CCTCCAGCGC CGAGATCCCC ATCGGCATCT ACAGGACCGA GTGCCATGTC 
TTCTCGGAGC CCCATGACGT CAGAGCCCAG TCTCAGATCT CGGTGAACAT GGAGGACTGC 
GAGGATACTC GGGAGGCCAA GGGACCCTGG GGCACACGAG CTGCATCTGG CAGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCAGTGGAGC ACCCACTACT ACGTCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGTCGC AAGAGCACCA AGCAGGCAGG GAAGGCACCT 
GTGGCCACAG ACTGGATCAC CCAGCAGCGG CTCAGCCTGT ACCGACGCTC AGAGCGCCAG 
GAGCTCTCAG AGCTGGTCAA GAACCGAATG AAGCACCTGG GACTGCCCAC CACTGGCTAT 
GATGAGATGA ACGATCATCA CCAGAACACC CTTTCCTATG TACTCATCAA CCCCCCGCCA 
GACACAAGAC TGGAACCCAA CGACATTGTG TACCTCATCC GTTCCGACCC CCTGGCCCAT 
GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT 
CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_017317210.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172699.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OMu80193
Clone ID Related Accession (Same CDS sequence)	XM_017317210.1 , XM_017317210.2
Accession Version	XM_017317210.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACA GTAAATTCTT CCGGGAGTAT GAGGGCAAGA GCTTCACCTA CGCAGCCTTC 
CACGCGCACA AGAAATATGG GGTGTGCCTC ATCGGGCTGA AGCGTGAGGA GAACAAGAGT 
ATCCTGCTGA ACCCAGGACC ACGGCACATC CTGGCTGCCT CCGACACCTG CTTCTATATC 
AATATTACCA AGGAGGAGAA CTCAGCTTTC ATCTTCAAAC AGGAGGAGAA GCAGAAGAGA 
CGGGGCCTTG CAGGGCAGGC ACTATATGAA GGGCCCTCCC GGCTCCCAGT GCATAGCATC 
ATCGCCTCTA TGGGGACAGT GGCCATGGAC CTGCAGAACA CAGATTGCCG GCCCTCCCAG 
GGTGGCAGTG GCGGGGACGG CACAAAGCTG ACTCTGCCCA CCGAGAACGG CTCTGGTAGT 
CGACGCCCCA GCATCGCACC CGTTCTGGAG TTGGCAGACA GCTCAGCCCT GTTGCCCTGC 
GACCTGCTGA GTGACCAGTC AGAGGATGAG GTGACACCCT CGGACGACGA GGGGCTCTCT 
GTGGTTGAGT ACGTGAAGGG CTATCCCCCC AACTCACCCT ACATTGGCAG CTCCCCGACT 
TTATGCCACC TCCTGCCTGT GAAAGCCCCC TTCTGCTGCC TGCGGTTGGA CAAGGGCTGC 
AAACACAACA GCTACGAGGA TGCCAAGGCC TATGGGTTCA AGAACAAGCT GATTATTGTC 
TCTGCTGAGA CGGCAGGCAA CGGGCTCTAC AACTTCATCG TGCCTCTGCG CGCCTACTAC 
CGGTCCCGCC GGGAGCTCAA CCCTATCGTG TTGCTGCTTG ACAACAAGCC TGACCACCAC 
TTCCTGGAGG CCATCTGTTG CTTCCCCATG GTCTACTACA TGGAGGGATC CGTGGACAAC 
CTGGACAGCT TACTGCAGTG TGGCATCATC TATGCTGACA ACCTGGTGGT GGTGGACAAG 
GAGAGCACTA TGAGCGCTGA AGAGGACTAC ATGGCAGATG CCAAGACCAT CGTCAATGTG 
CAAACCATGT TCCGGCTTTT CCCCAGTCTC AGCATCACCA CGGAGCTCAC ACACCCTTCC 
AACATGCGGT TCATGCAGTT CCGTGCCAAG GACAGCTACT CTCTGGCTCT TTCCAAACTT 
GAAAAGCAAG AACGGGAGAA CGGCTCCAAC CTGGCCTTCA TGTTCCGCCT GCCATTTGCT 
GCTGGTCGAG TATTTAGTAT CAGCATGTTG GATACACTGC TCTACCAGTC CTTCGTGAAG 
GACTACATGA TCACCATCAC CAGGCTGCTC TTGGGCCTTG ATACTACACC AGGCTCCGGC 
TACCTCTGTG CAATGAAGGT AACCGAGGAC GACCTGTGGA TCCGCACTTA CGGCCGCCTC 
TTCCAGAAAC TCTGCTCCTC CAGCGCCGAG ATCCCCATCG GCATCTACAG GACCGAGTGC 
CATGTCTTCT CGGAGCCCCA TGACGTCAGA GCCCAGGGGG GCCCAGACAT AGCCCTCCTG 
CCTGCCCAGT CTCAGATCTC GGTGAACATG GAGGACTGCG AGGATACTCG GGAGGCCAAG 
GGACCCTGGG GCACACGAGC TGCATCTGGC AGTGGCAGCA CCCATGGCCG TCACGGGGGC 
AGTGCTGACC CAGTGGAGCA CCCACTACTA CGTCGCAAGA GCCTGCAGTG GGCCCGCAAG 
CTGAGTCGCA AGAGCACCAA GCAGGCAGGG AAGGCACCTG TGGCCACAGA CTGGATCACC 
CAGCAGCGGC TCAGCCTGTA CCGACGCTCA GAGCGCCAGG AGCTCTCAGA GCTGGTCAAG 
AACCGAATGA AGCACCTGGG ACTGCCCACC ACTGGCTATG AGGACGTAGC AAATTTAACA 
GCCAGTGATG TGATGAATCG GGTAAACCTG GGATATTTGC AAGATGAGAT GAACGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAACCC 
AACGACATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAGCTC TCATCCTGTA ATCCTGAGAC CAGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172699.1
CDS	325..2436
Translation	MGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_017317210.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317210.1

ORF Insert Sequence:

ATGGGTGACA GTAAATTCTT CCGGGAGTAT GAGGGCAAGA GCTTCACCTA CGCAGCCTTC 
CACGCGCACA AGAAATATGG GGTGTGCCTC ATCGGGCTGA AGCGTGAGGA GAACAAGAGT 
ATCCTGCTGA ACCCAGGACC ACGGCACATC CTGGCTGCCT CCGACACCTG CTTCTATATC 
AATATTACCA AGGAGGAGAA CTCAGCTTTC ATCTTCAAAC AGGAGGAGAA GCAGAAGAGA 
CGGGGCCTTG CAGGGCAGGC ACTATATGAA GGGCCCTCCC GGCTCCCAGT GCATAGCATC 
ATCGCCTCTA TGGGGACAGT GGCCATGGAC CTGCAGAACA CAGATTGCCG GCCCTCCCAG 
GGTGGCAGTG GCGGGGACGG CACAAAGCTG ACTCTGCCCA CCGAGAACGG CTCTGGTAGT 
CGACGCCCCA GCATCGCACC CGTTCTGGAG TTGGCAGACA GCTCAGCCCT GTTGCCCTGC 
GACCTGCTGA GTGACCAGTC AGAGGATGAG GTGACACCCT CGGACGACGA GGGGCTCTCT 
GTGGTTGAGT ACGTGAAGGG CTATCCCCCC AACTCACCCT ACATTGGCAG CTCCCCGACT 
TTATGCCACC TCCTGCCTGT GAAAGCCCCC TTCTGCTGCC TGCGGTTGGA CAAGGGCTGC 
AAACACAACA GCTACGAGGA TGCCAAGGCC TATGGGTTCA AGAACAAGCT GATTATTGTC 
TCTGCTGAGA CGGCAGGCAA CGGGCTCTAC AACTTCATCG TGCCTCTGCG CGCCTACTAC 
CGGTCCCGCC GGGAGCTCAA CCCTATCGTG TTGCTGCTTG ACAACAAGCC TGACCACCAC 
TTCCTGGAGG CCATCTGTTG CTTCCCCATG GTCTACTACA TGGAGGGATC CGTGGACAAC 
CTGGACAGCT TACTGCAGTG TGGCATCATC TATGCTGACA ACCTGGTGGT GGTGGACAAG 
GAGAGCACTA TGAGCGCTGA AGAGGACTAC ATGGCAGATG CCAAGACCAT CGTCAATGTG 
CAAACCATGT TCCGGCTTTT CCCCAGTCTC AGCATCACCA CGGAGCTCAC ACACCCTTCC 
AACATGCGGT TCATGCAGTT CCGTGCCAAG GACAGCTACT CTCTGGCTCT TTCCAAACTT 
GAAAAGCAAG AACGGGAGAA CGGCTCCAAC CTGGCCTTCA TGTTCCGCCT GCCATTTGCT 
GCTGGTCGAG TATTTAGTAT CAGCATGTTG GATACACTGC TCTACCAGTC CTTCGTGAAG 
GACTACATGA TCACCATCAC CAGGCTGCTC TTGGGCCTTG ATACTACACC AGGCTCCGGC 
TACCTCTGTG CAATGAAGGT AACCGAGGAC GACCTGTGGA TCCGCACTTA CGGCCGCCTC 
TTCCAGAAAC TCTGCTCCTC CAGCGCCGAG ATCCCCATCG GCATCTACAG GACCGAGTGC 
CATGTCTTCT CGGAGCCCCA TGACGTCAGA GCCCAGGGGG GCCCAGACAT AGCCCTCCTG 
CCTGCCCAGT CTCAGATCTC GGTGAACATG GAGGACTGCG AGGATACTCG GGAGGCCAAG 
GGACCCTGGG GCACACGAGC TGCATCTGGC AGTGGCAGCA CCCATGGCCG TCACGGGGGC 
AGTGCTGACC CAGTGGAGCA CCCACTACTA CGTCGCAAGA GCCTGCAGTG GGCCCGCAAG 
CTGAGTCGCA AGAGCACCAA GCAGGCAGGG AAGGCACCTG TGGCCACAGA CTGGATCACC 
CAGCAGCGGC TCAGCCTGTA CCGACGCTCA GAGCGCCAGG AGCTCTCAGA GCTGGTCAAG 
AACCGAATGA AGCACCTGGG ACTGCCCACC ACTGGCTATG AGGACGTAGC AAATTTAACA 
GCCAGTGATG TGATGAATCG GGTAAACCTG GGATATTTGC AAGATGAGAT GAACGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAACCC 
AACGACATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAGCTC TCATCCTGTA ATCCTGAGAC CAGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_017317210.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172699.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X30, mRNA.

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CloneID	OMu80193
Clone ID Related Accession (Same CDS sequence)	XM_017317210.1 , XM_017317210.2
Accession Version	XM_017317210.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317210.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACA GTAAATTCTT CCGGGAGTAT GAGGGCAAGA GCTTCACCTA CGCAGCCTTC 
CACGCGCACA AGAAATATGG GGTGTGCCTC ATCGGGCTGA AGCGTGAGGA GAACAAGAGT 
ATCCTGCTGA ACCCAGGACC ACGGCACATC CTGGCTGCCT CCGACACCTG CTTCTATATC 
AATATTACCA AGGAGGAGAA CTCAGCTTTC ATCTTCAAAC AGGAGGAGAA GCAGAAGAGA 
CGGGGCCTTG CAGGGCAGGC ACTATATGAA GGGCCCTCCC GGCTCCCAGT GCATAGCATC 
ATCGCCTCTA TGGGGACAGT GGCCATGGAC CTGCAGAACA CAGATTGCCG GCCCTCCCAG 
GGTGGCAGTG GCGGGGACGG CACAAAGCTG ACTCTGCCCA CCGAGAACGG CTCTGGTAGT 
CGACGCCCCA GCATCGCACC CGTTCTGGAG TTGGCAGACA GCTCAGCCCT GTTGCCCTGC 
GACCTGCTGA GTGACCAGTC AGAGGATGAG GTGACACCCT CGGACGACGA GGGGCTCTCT 
GTGGTTGAGT ACGTGAAGGG CTATCCCCCC AACTCACCCT ACATTGGCAG CTCCCCGACT 
TTATGCCACC TCCTGCCTGT GAAAGCCCCC TTCTGCTGCC TGCGGTTGGA CAAGGGCTGC 
AAACACAACA GCTACGAGGA TGCCAAGGCC TATGGGTTCA AGAACAAGCT GATTATTGTC 
TCTGCTGAGA CGGCAGGCAA CGGGCTCTAC AACTTCATCG TGCCTCTGCG CGCCTACTAC 
CGGTCCCGCC GGGAGCTCAA CCCTATCGTG TTGCTGCTTG ACAACAAGCC TGACCACCAC 
TTCCTGGAGG CCATCTGTTG CTTCCCCATG GTCTACTACA TGGAGGGATC CGTGGACAAC 
CTGGACAGCT TACTGCAGTG TGGCATCATC TATGCTGACA ACCTGGTGGT GGTGGACAAG 
GAGAGCACTA TGAGCGCTGA AGAGGACTAC ATGGCAGATG CCAAGACCAT CGTCAATGTG 
CAAACCATGT TCCGGCTTTT CCCCAGTCTC AGCATCACCA CGGAGCTCAC ACACCCTTCC 
AACATGCGGT TCATGCAGTT CCGTGCCAAG GACAGCTACT CTCTGGCTCT TTCCAAACTT 
GAAAAGCAAG AACGGGAGAA CGGCTCCAAC CTGGCCTTCA TGTTCCGCCT GCCATTTGCT 
GCTGGTCGAG TATTTAGTAT CAGCATGTTG GATACACTGC TCTACCAGTC CTTCGTGAAG 
GACTACATGA TCACCATCAC CAGGCTGCTC TTGGGCCTTG ATACTACACC AGGCTCCGGC 
TACCTCTGTG CAATGAAGGT AACCGAGGAC GACCTGTGGA TCCGCACTTA CGGCCGCCTC 
TTCCAGAAAC TCTGCTCCTC CAGCGCCGAG ATCCCCATCG GCATCTACAG GACCGAGTGC 
CATGTCTTCT CGGAGCCCCA TGACGTCAGA GCCCAGGGGG GCCCAGACAT AGCCCTCCTG 
CCTGCCCAGT CTCAGATCTC GGTGAACATG GAGGACTGCG AGGATACTCG GGAGGCCAAG 
GGACCCTGGG GCACACGAGC TGCATCTGGC AGTGGCAGCA CCCATGGCCG TCACGGGGGC 
AGTGCTGACC CAGTGGAGCA CCCACTACTA CGTCGCAAGA GCCTGCAGTG GGCCCGCAAG 
CTGAGTCGCA AGAGCACCAA GCAGGCAGGG AAGGCACCTG TGGCCACAGA CTGGATCACC 
CAGCAGCGGC TCAGCCTGTA CCGACGCTCA GAGCGCCAGG AGCTCTCAGA GCTGGTCAAG 
AACCGAATGA AGCACCTGGG ACTGCCCACC ACTGGCTATG AGGACGTAGC AAATTTAACA 
GCCAGTGATG TGATGAATCG GGTAAACCTG GGATATTTGC AAGATGAGAT GAACGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAACCC 
AACGACATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAGCTC TCATCCTGTA ATCCTGAGAC CAGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172699.1
CDS	325..2436
Translation	MGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_017317210.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317210.2

ORF Insert Sequence:

ATGGGTGACA GTAAATTCTT CCGGGAGTAT GAGGGCAAGA GCTTCACCTA CGCAGCCTTC 
CACGCGCACA AGAAATATGG GGTGTGCCTC ATCGGGCTGA AGCGTGAGGA GAACAAGAGT 
ATCCTGCTGA ACCCAGGACC ACGGCACATC CTGGCTGCCT CCGACACCTG CTTCTATATC 
AATATTACCA AGGAGGAGAA CTCAGCTTTC ATCTTCAAAC AGGAGGAGAA GCAGAAGAGA 
CGGGGCCTTG CAGGGCAGGC ACTATATGAA GGGCCCTCCC GGCTCCCAGT GCATAGCATC 
ATCGCCTCTA TGGGGACAGT GGCCATGGAC CTGCAGAACA CAGATTGCCG GCCCTCCCAG 
GGTGGCAGTG GCGGGGACGG CACAAAGCTG ACTCTGCCCA CCGAGAACGG CTCTGGTAGT 
CGACGCCCCA GCATCGCACC CGTTCTGGAG TTGGCAGACA GCTCAGCCCT GTTGCCCTGC 
GACCTGCTGA GTGACCAGTC AGAGGATGAG GTGACACCCT CGGACGACGA GGGGCTCTCT 
GTGGTTGAGT ACGTGAAGGG CTATCCCCCC AACTCACCCT ACATTGGCAG CTCCCCGACT 
TTATGCCACC TCCTGCCTGT GAAAGCCCCC TTCTGCTGCC TGCGGTTGGA CAAGGGCTGC 
AAACACAACA GCTACGAGGA TGCCAAGGCC TATGGGTTCA AGAACAAGCT GATTATTGTC 
TCTGCTGAGA CGGCAGGCAA CGGGCTCTAC AACTTCATCG TGCCTCTGCG CGCCTACTAC 
CGGTCCCGCC GGGAGCTCAA CCCTATCGTG TTGCTGCTTG ACAACAAGCC TGACCACCAC 
TTCCTGGAGG CCATCTGTTG CTTCCCCATG GTCTACTACA TGGAGGGATC CGTGGACAAC 
CTGGACAGCT TACTGCAGTG TGGCATCATC TATGCTGACA ACCTGGTGGT GGTGGACAAG 
GAGAGCACTA TGAGCGCTGA AGAGGACTAC ATGGCAGATG CCAAGACCAT CGTCAATGTG 
CAAACCATGT TCCGGCTTTT CCCCAGTCTC AGCATCACCA CGGAGCTCAC ACACCCTTCC 
AACATGCGGT TCATGCAGTT CCGTGCCAAG GACAGCTACT CTCTGGCTCT TTCCAAACTT 
GAAAAGCAAG AACGGGAGAA CGGCTCCAAC CTGGCCTTCA TGTTCCGCCT GCCATTTGCT 
GCTGGTCGAG TATTTAGTAT CAGCATGTTG GATACACTGC TCTACCAGTC CTTCGTGAAG 
GACTACATGA TCACCATCAC CAGGCTGCTC TTGGGCCTTG ATACTACACC AGGCTCCGGC 
TACCTCTGTG CAATGAAGGT AACCGAGGAC GACCTGTGGA TCCGCACTTA CGGCCGCCTC 
TTCCAGAAAC TCTGCTCCTC CAGCGCCGAG ATCCCCATCG GCATCTACAG GACCGAGTGC 
CATGTCTTCT CGGAGCCCCA TGACGTCAGA GCCCAGGGGG GCCCAGACAT AGCCCTCCTG 
CCTGCCCAGT CTCAGATCTC GGTGAACATG GAGGACTGCG AGGATACTCG GGAGGCCAAG 
GGACCCTGGG GCACACGAGC TGCATCTGGC AGTGGCAGCA CCCATGGCCG TCACGGGGGC 
AGTGCTGACC CAGTGGAGCA CCCACTACTA CGTCGCAAGA GCCTGCAGTG GGCCCGCAAG 
CTGAGTCGCA AGAGCACCAA GCAGGCAGGG AAGGCACCTG TGGCCACAGA CTGGATCACC 
CAGCAGCGGC TCAGCCTGTA CCGACGCTCA GAGCGCCAGG AGCTCTCAGA GCTGGTCAAG 
AACCGAATGA AGCACCTGGG ACTGCCCACC ACTGGCTATG AGGACGTAGC AAATTTAACA 
GCCAGTGATG TGATGAATCG GGTAAACCTG GGATATTTGC AAGATGAGAT GAACGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAACCC 
AACGACATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAGCTC TCATCCTGTA ATCCTGAGAC CAGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_030249688.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105548.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X25, mRNA.

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CloneID	OMu87791
Clone ID Related Accession (Same CDS sequence)	XM_030249688.1 , XM_030249691.1
Accession Version	XM_030249688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACG GAGAGGCCTG CTTTATCCTC AGCAGCAGGA ATGAGGTGGA CCGCACAGCT 
GCGGATCACC AGACCATCCT TCGAGCCTGG GCTGTGAAGG ACTTTGCCCC CAACTGTCCC 
CTCTATGTCC AGATCCTCAA GCCCGAAAAC AAGTTTCACG TCAAATTTGC TGACCACGTG 
GTATGCGAGG AAGAGTGCAA GTACGCCATG CTGGCCCTGA ACTGCATCTG CCCGGCCACC 
TCCACCCTCA TCACCCTGCT GGTGCACACG TCCCGTGGCC AGGAAGGACA GGAGTCTCCC 
GAGCAGTGGC AGCGCACGTA CGGGAGGTGC TCGGGCAACG AGGTGTACCA CATTCGCATG 
GGTGACAGTA AATTCTTCCG GGAGTATGAG GGCAAGAGCT TCACCTACGC AGCCTTCCAC 
GCGCACAAGA AATATGGGGT GTGCCTCATC GGGCTGAAGC GTGAGGAGAA CAAGAGTATC 
CTGCTGAACC CAGGACCACG GCACATCCTG GCTGCCTCCG ACACCTGCTT CTATATCAAT 
ATTACCAAGG AGGAGAACTC AGCTTTCATC TTCAAACAGG AGGAGAAGCA GAAGAGACGG 
GGCCTTGCAG GGCAGGCACT ATATGAAGGG CCCTCCCGGC TCCCAGTGCA TAGCATCATC 
GCCTCTATGG GGACAGTGGC CATGGACCTG CAGAACACAG ATTGCCGGCC CTCCCAGGGT 
GGCAGTGGCG GGGACGGCAC AAAGCTGACT CTGCCCACCG AGAACGGCTC TGGTAGTCGA 
CGCCCCAGCA TCGCACCCGT TCTGGAGTTG GCAGACAGCT CAGCCCTGTT GCCCTGCGAC 
CTGCTGAGTG ACCAGTCAGA GGATGAGGTG ACACCCTCGG ACGACGAGGG GCTCTCTGTG 
GTTGAGTACG TGAAGGGCTA TCCCCCCAAC TCACCCTACA TTGGCAGCTC CCCGACTTTA 
TGCCACCTCC TGCCTGTGAA AGCCCCCTTC TGCTGCCTGC GGTTGGACAA GGGCTGCAAA 
CACAACAGCT ACGAGGATGC CAAGGCCTAT GGGTTCAAGA ACAAGCTGAT TATTGTCTCT 
GCTGAGACGG CAGGCAACGG GCTCTACAAC TTCATCGTGC CTCTGCGCGC CTACTACCGG 
TCCCGCCGGG AGCTCAACCC TATCGTGTTG CTGCTTGACA ACAAGCCTGA CCACCACTTC 
CTGGAGGCCA TCTGTTGCTT CCCCATGGTC TACTACATGG AGGGATCCGT GGACAACCTG 
GACAGCTTAC TGCAGTGTGG CATCATCTAT GCTGACAACC TGGTGGTGGT GGACAAGGAG 
AGCACTATGA GCGCTGAAGA GGACTACATG GCAGATGCCA AGACCATCGT CAATGTGCAA 
ACCATGTTCC GGCTTTTCCC CAGTCTCAGC ATCACCACGG AGCTCACACA CCCTTCCAAC 
ATGCGGTTCA TGCAGTTCCG TGCCAAGGAC AGCTACTCTC TGGCTCTTTC CAAACTTGAA 
AAGCAAGAAC GGGAGAACGG CTCCAACCTG GCCTTCATGT TCCGCCTGCC ATTTGCTGCT 
GGTCGAGTAT TTAGTATCAG CATGTTGGAT ACACTGCTCT ACCAGTCCTT CGTGAAGGAC 
TACATGATCA CCATCACCAG GCTGCTCTTG GGCCTTGATA CTACACCAGG CTCCGGCTAC 
CTCTGTGCAA TGAAGGTAAC CGAGGACGAC CTGTGGATCC GCACTTACGG CCGCCTCTTC 
CAGAAACTCT GCTCCTCCAG CGCCGAGATC CCCATCGGCA TCTACAGGAC CGAGTGCCAT 
GTCTTCTCGG AGCCCCATGA CGTCAGAGCC CAGGGGGGCC CAGACATAGC CCTCCTGCCT 
GCCCAGTCTC AGATCTCGGT GAACATGGAG GACTGCGAGG ATACTCGGGA GGCCAAGGGA 
CCCTGGGGCA CACGAGCTGC ATCTGGCAGT GGCAGCACCC ATGGCCGTCA CGGGGGCAGT 
GCTGACCCAG TGGAGCACCC ACTACTACGT CGCAAGAGCC TGCAGTGGGC CCGCAAGCTG 
AGTCGCAAGA GCACCAAGCA GGCAGGGAAG GCACCTGTGG CCACAGACTG GATCACCCAG 
CAGCGGCTCA GCCTGTACCG ACGCTCAGAG CGCCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGAATGAAGC ACCTGGGACT GCCCACCACT GGCTATGAGG ACGTAGCAAA TTTAACAGCC 
AGTGATGTGA TGAATCGGGT AAACCTGGGA TATTTGCAAG ATGAGATGAA CGATCATCAC 
CAGAACACCC TTTCCTATGT ACTCATCAAC CCCCCGCCAG ACACAAGACT GGAACCCAAC 
GACATTGTGT ACCTCATCCG TTCCGACCCC CTGGCCCATG TGGCCAGCAG CTCCCAGAGT 
CGGAAAAGCA GCTGCAGCAA CAAGCTCTCA TCCTGTAATC CTGAGACCAG GGATGAGACC 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105548.1
CDS	3319..5787
Translation	MDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_030249688.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249688.1

ORF Insert Sequence:

ATGGACAACG GAGAGGCCTG CTTTATCCTC AGCAGCAGGA ATGAGGTGGA CCGCACAGCT 
GCGGATCACC AGACCATCCT TCGAGCCTGG GCTGTGAAGG ACTTTGCCCC CAACTGTCCC 
CTCTATGTCC AGATCCTCAA GCCCGAAAAC AAGTTTCACG TCAAATTTGC TGACCACGTG 
GTATGCGAGG AAGAGTGCAA GTACGCCATG CTGGCCCTGA ACTGCATCTG CCCGGCCACC 
TCCACCCTCA TCACCCTGCT GGTGCACACG TCCCGTGGCC AGGAAGGACA GGAGTCTCCC 
GAGCAGTGGC AGCGCACGTA CGGGAGGTGC TCGGGCAACG AGGTGTACCA CATTCGCATG 
GGTGACAGTA AATTCTTCCG GGAGTATGAG GGCAAGAGCT TCACCTACGC AGCCTTCCAC 
GCGCACAAGA AATATGGGGT GTGCCTCATC GGGCTGAAGC GTGAGGAGAA CAAGAGTATC 
CTGCTGAACC CAGGACCACG GCACATCCTG GCTGCCTCCG ACACCTGCTT CTATATCAAT 
ATTACCAAGG AGGAGAACTC AGCTTTCATC TTCAAACAGG AGGAGAAGCA GAAGAGACGG 
GGCCTTGCAG GGCAGGCACT ATATGAAGGG CCCTCCCGGC TCCCAGTGCA TAGCATCATC 
GCCTCTATGG GGACAGTGGC CATGGACCTG CAGAACACAG ATTGCCGGCC CTCCCAGGGT 
GGCAGTGGCG GGGACGGCAC AAAGCTGACT CTGCCCACCG AGAACGGCTC TGGTAGTCGA 
CGCCCCAGCA TCGCACCCGT TCTGGAGTTG GCAGACAGCT CAGCCCTGTT GCCCTGCGAC 
CTGCTGAGTG ACCAGTCAGA GGATGAGGTG ACACCCTCGG ACGACGAGGG GCTCTCTGTG 
GTTGAGTACG TGAAGGGCTA TCCCCCCAAC TCACCCTACA TTGGCAGCTC CCCGACTTTA 
TGCCACCTCC TGCCTGTGAA AGCCCCCTTC TGCTGCCTGC GGTTGGACAA GGGCTGCAAA 
CACAACAGCT ACGAGGATGC CAAGGCCTAT GGGTTCAAGA ACAAGCTGAT TATTGTCTCT 
GCTGAGACGG CAGGCAACGG GCTCTACAAC TTCATCGTGC CTCTGCGCGC CTACTACCGG 
TCCCGCCGGG AGCTCAACCC TATCGTGTTG CTGCTTGACA ACAAGCCTGA CCACCACTTC 
CTGGAGGCCA TCTGTTGCTT CCCCATGGTC TACTACATGG AGGGATCCGT GGACAACCTG 
GACAGCTTAC TGCAGTGTGG CATCATCTAT GCTGACAACC TGGTGGTGGT GGACAAGGAG 
AGCACTATGA GCGCTGAAGA GGACTACATG GCAGATGCCA AGACCATCGT CAATGTGCAA 
ACCATGTTCC GGCTTTTCCC CAGTCTCAGC ATCACCACGG AGCTCACACA CCCTTCCAAC 
ATGCGGTTCA TGCAGTTCCG TGCCAAGGAC AGCTACTCTC TGGCTCTTTC CAAACTTGAA 
AAGCAAGAAC GGGAGAACGG CTCCAACCTG GCCTTCATGT TCCGCCTGCC ATTTGCTGCT 
GGTCGAGTAT TTAGTATCAG CATGTTGGAT ACACTGCTCT ACCAGTCCTT CGTGAAGGAC 
TACATGATCA CCATCACCAG GCTGCTCTTG GGCCTTGATA CTACACCAGG CTCCGGCTAC 
CTCTGTGCAA TGAAGGTAAC CGAGGACGAC CTGTGGATCC GCACTTACGG CCGCCTCTTC 
CAGAAACTCT GCTCCTCCAG CGCCGAGATC CCCATCGGCA TCTACAGGAC CGAGTGCCAT 
GTCTTCTCGG AGCCCCATGA CGTCAGAGCC CAGGGGGGCC CAGACATAGC CCTCCTGCCT 
GCCCAGTCTC AGATCTCGGT GAACATGGAG GACTGCGAGG ATACTCGGGA GGCCAAGGGA 
CCCTGGGGCA CACGAGCTGC ATCTGGCAGT GGCAGCACCC ATGGCCGTCA CGGGGGCAGT 
GCTGACCCAG TGGAGCACCC ACTACTACGT CGCAAGAGCC TGCAGTGGGC CCGCAAGCTG 
AGTCGCAAGA GCACCAAGCA GGCAGGGAAG GCACCTGTGG CCACAGACTG GATCACCCAG 
CAGCGGCTCA GCCTGTACCG ACGCTCAGAG CGCCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGAATGAAGC ACCTGGGACT GCCCACCACT GGCTATGAGG ACGTAGCAAA TTTAACAGCC 
AGTGATGTGA TGAATCGGGT AAACCTGGGA TATTTGCAAG ATGAGATGAA CGATCATCAC 
CAGAACACCC TTTCCTATGT ACTCATCAAC CCCCCGCCAG ACACAAGACT GGAACCCAAC 
GACATTGTGT ACCTCATCCG TTCCGACCCC CTGGCCCATG TGGCCAGCAG CTCCCAGAGT 
CGGAAAAGCA GCTGCAGCAA CAAGCTCTCA TCCTGTAATC CTGAGACCAG GGATGAGACC 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_030249691.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105551.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OMu87791
Clone ID Related Accession (Same CDS sequence)	XM_030249688.1 , XM_030249691.1
Accession Version	XM_030249691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACG GAGAGGCCTG CTTTATCCTC AGCAGCAGGA ATGAGGTGGA CCGCACAGCT 
GCGGATCACC AGACCATCCT TCGAGCCTGG GCTGTGAAGG ACTTTGCCCC CAACTGTCCC 
CTCTATGTCC AGATCCTCAA GCCCGAAAAC AAGTTTCACG TCAAATTTGC TGACCACGTG 
GTATGCGAGG AAGAGTGCAA GTACGCCATG CTGGCCCTGA ACTGCATCTG CCCGGCCACC 
TCCACCCTCA TCACCCTGCT GGTGCACACG TCCCGTGGCC AGGAAGGACA GGAGTCTCCC 
GAGCAGTGGC AGCGCACGTA CGGGAGGTGC TCGGGCAACG AGGTGTACCA CATTCGCATG 
GGTGACAGTA AATTCTTCCG GGAGTATGAG GGCAAGAGCT TCACCTACGC AGCCTTCCAC 
GCGCACAAGA AATATGGGGT GTGCCTCATC GGGCTGAAGC GTGAGGAGAA CAAGAGTATC 
CTGCTGAACC CAGGACCACG GCACATCCTG GCTGCCTCCG ACACCTGCTT CTATATCAAT 
ATTACCAAGG AGGAGAACTC AGCTTTCATC TTCAAACAGG AGGAGAAGCA GAAGAGACGG 
GGCCTTGCAG GGCAGGCACT ATATGAAGGG CCCTCCCGGC TCCCAGTGCA TAGCATCATC 
GCCTCTATGG GGACAGTGGC CATGGACCTG CAGAACACAG ATTGCCGGCC CTCCCAGGGT 
GGCAGTGGCG GGGACGGCAC AAAGCTGACT CTGCCCACCG AGAACGGCTC TGGTAGTCGA 
CGCCCCAGCA TCGCACCCGT TCTGGAGTTG GCAGACAGCT CAGCCCTGTT GCCCTGCGAC 
CTGCTGAGTG ACCAGTCAGA GGATGAGGTG ACACCCTCGG ACGACGAGGG GCTCTCTGTG 
GTTGAGTACG TGAAGGGCTA TCCCCCCAAC TCACCCTACA TTGGCAGCTC CCCGACTTTA 
TGCCACCTCC TGCCTGTGAA AGCCCCCTTC TGCTGCCTGC GGTTGGACAA GGGCTGCAAA 
CACAACAGCT ACGAGGATGC CAAGGCCTAT GGGTTCAAGA ACAAGCTGAT TATTGTCTCT 
GCTGAGACGG CAGGCAACGG GCTCTACAAC TTCATCGTGC CTCTGCGCGC CTACTACCGG 
TCCCGCCGGG AGCTCAACCC TATCGTGTTG CTGCTTGACA ACAAGCCTGA CCACCACTTC 
CTGGAGGCCA TCTGTTGCTT CCCCATGGTC TACTACATGG AGGGATCCGT GGACAACCTG 
GACAGCTTAC TGCAGTGTGG CATCATCTAT GCTGACAACC TGGTGGTGGT GGACAAGGAG 
AGCACTATGA GCGCTGAAGA GGACTACATG GCAGATGCCA AGACCATCGT CAATGTGCAA 
ACCATGTTCC GGCTTTTCCC CAGTCTCAGC ATCACCACGG AGCTCACACA CCCTTCCAAC 
ATGCGGTTCA TGCAGTTCCG TGCCAAGGAC AGCTACTCTC TGGCTCTTTC CAAACTTGAA 
AAGCAAGAAC GGGAGAACGG CTCCAACCTG GCCTTCATGT TCCGCCTGCC ATTTGCTGCT 
GGTCGAGTAT TTAGTATCAG CATGTTGGAT ACACTGCTCT ACCAGTCCTT CGTGAAGGAC 
TACATGATCA CCATCACCAG GCTGCTCTTG GGCCTTGATA CTACACCAGG CTCCGGCTAC 
CTCTGTGCAA TGAAGGTAAC CGAGGACGAC CTGTGGATCC GCACTTACGG CCGCCTCTTC 
CAGAAACTCT GCTCCTCCAG CGCCGAGATC CCCATCGGCA TCTACAGGAC CGAGTGCCAT 
GTCTTCTCGG AGCCCCATGA CGTCAGAGCC CAGGGGGGCC CAGACATAGC CCTCCTGCCT 
GCCCAGTCTC AGATCTCGGT GAACATGGAG GACTGCGAGG ATACTCGGGA GGCCAAGGGA 
CCCTGGGGCA CACGAGCTGC ATCTGGCAGT GGCAGCACCC ATGGCCGTCA CGGGGGCAGT 
GCTGACCCAG TGGAGCACCC ACTACTACGT CGCAAGAGCC TGCAGTGGGC CCGCAAGCTG 
AGTCGCAAGA GCACCAAGCA GGCAGGGAAG GCACCTGTGG CCACAGACTG GATCACCCAG 
CAGCGGCTCA GCCTGTACCG ACGCTCAGAG CGCCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGAATGAAGC ACCTGGGACT GCCCACCACT GGCTATGAGG ACGTAGCAAA TTTAACAGCC 
AGTGATGTGA TGAATCGGGT AAACCTGGGA TATTTGCAAG ATGAGATGAA CGATCATCAC 
CAGAACACCC TTTCCTATGT ACTCATCAAC CCCCCGCCAG ACACAAGACT GGAACCCAAC 
GACATTGTGT ACCTCATCCG TTCCGACCCC CTGGCCCATG TGGCCAGCAG CTCCCAGAGT 
CGGAAAAGCA GCTGCAGCAA CAAGCTCTCA TCCTGTAATC CTGAGACCAG GGATGAGACC 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105551.1
CDS	1430..3898
Translation	MDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_030249691.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249691.1

ORF Insert Sequence:

ATGGACAACG GAGAGGCCTG CTTTATCCTC AGCAGCAGGA ATGAGGTGGA CCGCACAGCT 
GCGGATCACC AGACCATCCT TCGAGCCTGG GCTGTGAAGG ACTTTGCCCC CAACTGTCCC 
CTCTATGTCC AGATCCTCAA GCCCGAAAAC AAGTTTCACG TCAAATTTGC TGACCACGTG 
GTATGCGAGG AAGAGTGCAA GTACGCCATG CTGGCCCTGA ACTGCATCTG CCCGGCCACC 
TCCACCCTCA TCACCCTGCT GGTGCACACG TCCCGTGGCC AGGAAGGACA GGAGTCTCCC 
GAGCAGTGGC AGCGCACGTA CGGGAGGTGC TCGGGCAACG AGGTGTACCA CATTCGCATG 
GGTGACAGTA AATTCTTCCG GGAGTATGAG GGCAAGAGCT TCACCTACGC AGCCTTCCAC 
GCGCACAAGA AATATGGGGT GTGCCTCATC GGGCTGAAGC GTGAGGAGAA CAAGAGTATC 
CTGCTGAACC CAGGACCACG GCACATCCTG GCTGCCTCCG ACACCTGCTT CTATATCAAT 
ATTACCAAGG AGGAGAACTC AGCTTTCATC TTCAAACAGG AGGAGAAGCA GAAGAGACGG 
GGCCTTGCAG GGCAGGCACT ATATGAAGGG CCCTCCCGGC TCCCAGTGCA TAGCATCATC 
GCCTCTATGG GGACAGTGGC CATGGACCTG CAGAACACAG ATTGCCGGCC CTCCCAGGGT 
GGCAGTGGCG GGGACGGCAC AAAGCTGACT CTGCCCACCG AGAACGGCTC TGGTAGTCGA 
CGCCCCAGCA TCGCACCCGT TCTGGAGTTG GCAGACAGCT CAGCCCTGTT GCCCTGCGAC 
CTGCTGAGTG ACCAGTCAGA GGATGAGGTG ACACCCTCGG ACGACGAGGG GCTCTCTGTG 
GTTGAGTACG TGAAGGGCTA TCCCCCCAAC TCACCCTACA TTGGCAGCTC CCCGACTTTA 
TGCCACCTCC TGCCTGTGAA AGCCCCCTTC TGCTGCCTGC GGTTGGACAA GGGCTGCAAA 
CACAACAGCT ACGAGGATGC CAAGGCCTAT GGGTTCAAGA ACAAGCTGAT TATTGTCTCT 
GCTGAGACGG CAGGCAACGG GCTCTACAAC TTCATCGTGC CTCTGCGCGC CTACTACCGG 
TCCCGCCGGG AGCTCAACCC TATCGTGTTG CTGCTTGACA ACAAGCCTGA CCACCACTTC 
CTGGAGGCCA TCTGTTGCTT CCCCATGGTC TACTACATGG AGGGATCCGT GGACAACCTG 
GACAGCTTAC TGCAGTGTGG CATCATCTAT GCTGACAACC TGGTGGTGGT GGACAAGGAG 
AGCACTATGA GCGCTGAAGA GGACTACATG GCAGATGCCA AGACCATCGT CAATGTGCAA 
ACCATGTTCC GGCTTTTCCC CAGTCTCAGC ATCACCACGG AGCTCACACA CCCTTCCAAC 
ATGCGGTTCA TGCAGTTCCG TGCCAAGGAC AGCTACTCTC TGGCTCTTTC CAAACTTGAA 
AAGCAAGAAC GGGAGAACGG CTCCAACCTG GCCTTCATGT TCCGCCTGCC ATTTGCTGCT 
GGTCGAGTAT TTAGTATCAG CATGTTGGAT ACACTGCTCT ACCAGTCCTT CGTGAAGGAC 
TACATGATCA CCATCACCAG GCTGCTCTTG GGCCTTGATA CTACACCAGG CTCCGGCTAC 
CTCTGTGCAA TGAAGGTAAC CGAGGACGAC CTGTGGATCC GCACTTACGG CCGCCTCTTC 
CAGAAACTCT GCTCCTCCAG CGCCGAGATC CCCATCGGCA TCTACAGGAC CGAGTGCCAT 
GTCTTCTCGG AGCCCCATGA CGTCAGAGCC CAGGGGGGCC CAGACATAGC CCTCCTGCCT 
GCCCAGTCTC AGATCTCGGT GAACATGGAG GACTGCGAGG ATACTCGGGA GGCCAAGGGA 
CCCTGGGGCA CACGAGCTGC ATCTGGCAGT GGCAGCACCC ATGGCCGTCA CGGGGGCAGT 
GCTGACCCAG TGGAGCACCC ACTACTACGT CGCAAGAGCC TGCAGTGGGC CCGCAAGCTG 
AGTCGCAAGA GCACCAAGCA GGCAGGGAAG GCACCTGTGG CCACAGACTG GATCACCCAG 
CAGCGGCTCA GCCTGTACCG ACGCTCAGAG CGCCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGAATGAAGC ACCTGGGACT GCCCACCACT GGCTATGAGG ACGTAGCAAA TTTAACAGCC 
AGTGATGTGA TGAATCGGGT AAACCTGGGA TATTTGCAAG ATGAGATGAA CGATCATCAC 
CAGAACACCC TTTCCTATGT ACTCATCAAC CCCCCGCCAG ACACAAGACT GGAACCCAAC 
GACATTGTGT ACCTCATCCG TTCCGACCCC CTGGCCCATG TGGCCAGCAG CTCCCAGAGT 
CGGAAAAGCA GCTGCAGCAA CAAGCTCTCA TCCTGTAATC CTGAGACCAG GGATGAGACC 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_030249700.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105560.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OMu87792
Clone ID Related Accession (Same CDS sequence)	XM_030249700.1
Accession Version	XM_030249700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGAA GAGTGCAAGT ACGCCATGCT GGCCCTGAAC TGCATCTGCC CGGCCACCTC 
CACCCTCATC ACCCTGCTGG TGCACACGTC CCGTGGCCAG TGAGTGCCCT GTGCCAGACC 
GACTACCACG CCCTGGTCAT CCCGATCAAC CCCACAAGGG AAGGACAGGA GTCTCCCGAG 
CAGTGGCAGC GCACGTACGG GAGGTGCTCG GGCAACGAGG TGTACCACAT TCGCATGGGT 
GACAGTAAAT TCTTCCGGGA GTATGAGGGC AAGAGCTTCA CCTACGCAGC CTTCCACGCG 
CACAAGAAAT ATGGGGTGTG CCTCATCGGG CTGAAGCGTG AGGAGAACAA GAGTATCCTG 
CTGAACCCAG GACCACGGCA CATCCTGGCT GCCTCCGACA CCTGCTTCTA TATCAATATT 
ACCAAGGAGG AGAACTCAGC TTTCATCTTC AAACAGGAGG AGAAGCAGAA GAGACGGGGC 
CTTGCAGGGC AGGCACTATA TGAAGGGCCC TCCCGGCTCC CAGTGCATAG CATCATCGCC 
TCTATGGGGA CAGTGGCCAT GGACCTGCAG AACACAGATT GCCGGCCCTC CCAGGGTGGC 
AGTGGCGGGG ACGGCACAAA GCTGACTCTG CCCACCGAGA ACGGCTCTGG TAGTCGACGC 
CCCAGCATCG CACCCGTTCT GGAGTTGGCA GACAGCTCAG CCCTGTTGCC CTGCGACCTG 
CTGAGTGACC AGTCAGAGGA TGAGGTGACA CCCTCGGACG ACGAGGGGCT CTCTGTGGTT 
GAGTACGTGA AGGGCTATCC CCCCAACTCA CCCTACATTG GCAGCTCCCC GACTTTATGC 
CACCTCCTGC CTGTGAAAGC CCCCTTCTGC TGCCTGCGGT TGGACAAGGG CTGCAAACAC 
AACAGCTACG AGGATGCCAA GGCCTATGGG TTCAAGAACA AGCTGATTAT TGTCTCTGCT 
GAGACGGCAG GCAACGGGCT CTACAACTTC ATCGTGCCTC TGCGCGCCTA CTACCGGTCC 
CGCCGGGAGC TCAACCCTAT CGTGTTGCTG CTTGACAACA ACCTGGACAG CTTACTGCAG 
TGTGGCATCA TCTATGCTGA CAACCTGGTG GTGGTGGACA AGGAGAGCAC TATGAGCGCT 
GAAGAGGACT ACATGGCAGA TGCCAAGACC ATCGTCAATG TGCAAACCAT GTTCCGGCTT 
TTCCCCAGTC TCAGCATCAC CACGGAGCTC ACACACCCTT CCAACATGCG GTTCATGCAG 
TTCCGTGCCA AGGACAGCTA CTCTCTGGCT CTTTCCAAAC TTGAAAAGCA AGAACGGGAG 
AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCATTTG CTGCTGGTCG AGTATTTAGT 
ATCAGCATGT TGGATACACT GCTCTACCAG TCCTTCGTGA AGGACTACAT GATCACCATC 
ACCAGGCTGC TCTTGGGCCT TGATACTACA CCAGGCTCCG GCTACCTCTG TGCAATGAAG 
GTAACCGAGG ACGACCTGTG GATCCGCACT TACGGCCGCC TCTTCCAGAA ACTCTGCTCC 
TCCAGCGCCG AGATCCCCAT CGGCATCTAC AGGACCGAGT GCCATGTCTT CTCGGAGCCC 
CATGACGTCA GAGCCCAGGG GGGCCCAGAC ATAGCCCTCC TGCCTGCCCA GTCTCAGATC 
TCGGTGAACA TGGAGGACTG CGAGGATACT CGGGAGGCCA AGGGACCCTG GGGCACACGA 
GCTGCATCTG GCAGTGGCAG CACCCATGGC CGTCACGGGG GCAGTGCTGA CCCAGTGGAG 
CACCCACTAC TACGTCGCAA GAGCCTGCAG TGGGCCCGCA AGCTGAGTCG CAAGAGCACC 
AAGCAGGCAG GGAAGGCACC TGTGGCCACA GACTGGATCA CCCAGCAGCG GCTCAGCCTG 
TACCGACGCT CAGAGCGCCA GGAGCTCTCA GAGCTGGTCA AGAACCGAAT GAAGCACCTG 
GGACTGCCCA CCACTGGCTA TGAGGACGTA GCAAATTTAA CAGCCAGTGA TGTGATGAAT 
CGGGTAAACC TGGGATATTT GCAAGATGAG ATGAACGATC ATCACCAGAA CACCCTTTCC 
TATGTACTCA TCAACCCCCC GCCAGACACA AGACTGGAAC CCAACGACAT TGTGTACCTC 
ATCCGTTCCG ACCCCCTGGC CCATGTGGCC AGCAGCTCCC AGAGTCGGAA AAGCAGCTGC 
AGCAACAAGC TCTCATCCTG TAATCCTGAG ACCAGGGATG AGACCCAGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105560.1
CDS	603..2876
Translation	MRGRVQVRHAGPELHLPGHLHPHHPAGAHVPWPVSALCQTDYHALVIPINPTREGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_030249700.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249700.1

ORF Insert Sequence:

ATGCGAGGAA GAGTGCAAGT ACGCCATGCT GGCCCTGAAC TGCATCTGCC CGGCCACCTC 
CACCCTCATC ACCCTGCTGG TGCACACGTC CCGTGGCCAG TGAGTGCCCT GTGCCAGACC 
GACTACCACG CCCTGGTCAT CCCGATCAAC CCCACAAGGG AAGGACAGGA GTCTCCCGAG 
CAGTGGCAGC GCACGTACGG GAGGTGCTCG GGCAACGAGG TGTACCACAT TCGCATGGGT 
GACAGTAAAT TCTTCCGGGA GTATGAGGGC AAGAGCTTCA CCTACGCAGC CTTCCACGCG 
CACAAGAAAT ATGGGGTGTG CCTCATCGGG CTGAAGCGTG AGGAGAACAA GAGTATCCTG 
CTGAACCCAG GACCACGGCA CATCCTGGCT GCCTCCGACA CCTGCTTCTA TATCAATATT 
ACCAAGGAGG AGAACTCAGC TTTCATCTTC AAACAGGAGG AGAAGCAGAA GAGACGGGGC 
CTTGCAGGGC AGGCACTATA TGAAGGGCCC TCCCGGCTCC CAGTGCATAG CATCATCGCC 
TCTATGGGGA CAGTGGCCAT GGACCTGCAG AACACAGATT GCCGGCCCTC CCAGGGTGGC 
AGTGGCGGGG ACGGCACAAA GCTGACTCTG CCCACCGAGA ACGGCTCTGG TAGTCGACGC 
CCCAGCATCG CACCCGTTCT GGAGTTGGCA GACAGCTCAG CCCTGTTGCC CTGCGACCTG 
CTGAGTGACC AGTCAGAGGA TGAGGTGACA CCCTCGGACG ACGAGGGGCT CTCTGTGGTT 
GAGTACGTGA AGGGCTATCC CCCCAACTCA CCCTACATTG GCAGCTCCCC GACTTTATGC 
CACCTCCTGC CTGTGAAAGC CCCCTTCTGC TGCCTGCGGT TGGACAAGGG CTGCAAACAC 
AACAGCTACG AGGATGCCAA GGCCTATGGG TTCAAGAACA AGCTGATTAT TGTCTCTGCT 
GAGACGGCAG GCAACGGGCT CTACAACTTC ATCGTGCCTC TGCGCGCCTA CTACCGGTCC 
CGCCGGGAGC TCAACCCTAT CGTGTTGCTG CTTGACAACA ACCTGGACAG CTTACTGCAG 
TGTGGCATCA TCTATGCTGA CAACCTGGTG GTGGTGGACA AGGAGAGCAC TATGAGCGCT 
GAAGAGGACT ACATGGCAGA TGCCAAGACC ATCGTCAATG TGCAAACCAT GTTCCGGCTT 
TTCCCCAGTC TCAGCATCAC CACGGAGCTC ACACACCCTT CCAACATGCG GTTCATGCAG 
TTCCGTGCCA AGGACAGCTA CTCTCTGGCT CTTTCCAAAC TTGAAAAGCA AGAACGGGAG 
AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCATTTG CTGCTGGTCG AGTATTTAGT 
ATCAGCATGT TGGATACACT GCTCTACCAG TCCTTCGTGA AGGACTACAT GATCACCATC 
ACCAGGCTGC TCTTGGGCCT TGATACTACA CCAGGCTCCG GCTACCTCTG TGCAATGAAG 
GTAACCGAGG ACGACCTGTG GATCCGCACT TACGGCCGCC TCTTCCAGAA ACTCTGCTCC 
TCCAGCGCCG AGATCCCCAT CGGCATCTAC AGGACCGAGT GCCATGTCTT CTCGGAGCCC 
CATGACGTCA GAGCCCAGGG GGGCCCAGAC ATAGCCCTCC TGCCTGCCCA GTCTCAGATC 
TCGGTGAACA TGGAGGACTG CGAGGATACT CGGGAGGCCA AGGGACCCTG GGGCACACGA 
GCTGCATCTG GCAGTGGCAG CACCCATGGC CGTCACGGGG GCAGTGCTGA CCCAGTGGAG 
CACCCACTAC TACGTCGCAA GAGCCTGCAG TGGGCCCGCA AGCTGAGTCG CAAGAGCACC 
AAGCAGGCAG GGAAGGCACC TGTGGCCACA GACTGGATCA CCCAGCAGCG GCTCAGCCTG 
TACCGACGCT CAGAGCGCCA GGAGCTCTCA GAGCTGGTCA AGAACCGAAT GAAGCACCTG 
GGACTGCCCA CCACTGGCTA TGAGGACGTA GCAAATTTAA CAGCCAGTGA TGTGATGAAT 
CGGGTAAACC TGGGATATTT GCAAGATGAG ATGAACGATC ATCACCAGAA CACCCTTTCC 
TATGTACTCA TCAACCCCCC GCCAGACACA AGACTGGAAC CCAACGACAT TGTGTACCTC 
ATCCGTTCCG ACCCCCTGGC CCATGTGGCC AGCAGCTCCC AGAGTCGGAA AAGCAGCTGC 
AGCAACAAGC TCTCATCCTG TAATCCTGAG ACCAGGGATG AGACCCAGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497891.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497954.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X16, mRNA.

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CloneID	OMu29451
Clone ID Related Accession (Same CDS sequence)	XM_006497891.3 , XM_006497891.4
Accession Version	XM_006497891.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497891.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGACC TGGACACGGA GGTGCTGCCC TTGCCGCCAA GGTACCGATT CCGTGACCTG 
CTTTTAGGAG ACCAGCCATT CCCGAACGAC GATAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GCAGGACTAC TACGTGGTCA TCCTGTGTCC CTCTGAAATG 
GACGTCCAGG TGCGCAGGGT GCTGCAGATT CCCCTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCTG CCCTCAAGGA CCAGGATCTC ATGCGAGCCA AGATGGACAA CGGAGAGGCC 
TGCTTTATCC TCAGCAGCAG GAATGAGGTG GACCGCACAG CTGCGGATCA CCAGACCATC 
CTTCGAGCCT GGGCTGTGAA GGACTTTGCC CCCAACTGTC CCCTCTATGT CCAGATCCTC 
AAGCCCGAAA ACAAGTTTCA CGTCAAATTT GCTGACCACG TGGTATGCGA GGAAGAGTGC 
AAGTACGCCA TGCTGGCCCT GAACTGCATC TGCCCGGCCA CCTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGTGG CCAGGAAGGA CAGGAGTCTC CCGAGCAGTG GCAGCGCACG 
TACGGGAGGT GCTCGGGCAA CGAGGTGTAC CACATTCGCA TGGGTGACAG TAAATTCTTC 
CGGGAGTATG AGGGCAAGAG CTTCACCTAC GCAGCCTTCC ACGCGCACAA GAAATATGGG 
GTGTGCCTCA TCGGGCTGAA GCGTGAGGAG AACAAGAGTA TCCTGCTGAA CCCAGGACCA 
CGGCACATCC TGGCTGCCTC CGACACCTGC TTCTATATCA ATATTACCAA GGAGGAGAAC 
TCAGCTTTCA TCTTCAAACA GGAGGAGAAG CAGAAGAGAC GGGGCCTTGC AGGGCAGGCA 
CTATATGAAG GGCCCTCCCG GCTCCCAGTG CATAGCATCA TCGCCTCTAT GGGGACAGTG 
GCCATGGACC TGCAGAACAC AGATTGCCGG CCCTCCCAGG GTGGCAGTGG CGGGGACGGC 
ACAAAGCTGA CTCTGCCCAC CGAGAACGGC TCTGGTAGTC GACGCCCCAG CATCGCACCC 
GTTCTGGAGT TGGCAGACAG CTCAGCCCTG TTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGATGAGG TGACACCCTC GGACGACGAG GGGCTCTCTG TGGTTGAGTA CGTGAAGGGC 
TATCCCCCCA ACTCACCCTA CATTGGCAGC TCCCCGACTT TATGCCACCT CCTGCCTGTG 
AAAGCCCCCT TCTGCTGCCT GCGGTTGGAC AAGGGCTGCA AACACAACAG CTACGAGGAT 
GCCAAGGCCT ATGGGTTCAA GAACAAGCTG ATTATTGTCT CTGCTGAGAC GGCAGGCAAC 
GGGCTCTACA ACTTCATCGT GCCTCTGCGC GCCTACTACC GGTCCCGCCG GGAGCTCAAC 
CCTATCGTGT TGCTGCTTGA CAACAAGCCT GACCACCACT TCCTGGAGGC CATCTGTTGC 
TTCCCCATGG TCTACTACAT GGAGGGATCC GTGGACAACC TGGACAGCTT ACTGCAGTGT 
GGCATCATCT ATGCTGACAA CCTGGTGGTG GTGGACAAGG AGAGCACTAT GAGCGCTGAA 
GAGGACTACA TGGCAGATGC CAAGACCATC GTCAATGTGC AAACCATGTT CCGGCTTTTC 
CCCAGTCTCA GCATCACCAC GGAGCTCACA CACCCTTCCA ACATGCGGTT CATGCAGTTC 
CGTGCCAAGG ACAGCTACTC TCTGGCTCTT TCCAAACTTG AAAAGCAAGA ACGGGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCATTTGCTG CTGGTCGAGT ATTTAGTATC 
AGCATGTTGG ATACACTGCT CTACCAGTCC TTCGTGAAGG ACTACATGAT CACCATCACC 
AGGCTGCTCT TGGGCCTTGA TACTACACCA GGCTCCGGCT ACCTCTGTGC AATGAAGGTA 
ACCGAGGACG ACCTGTGGAT CCGCACTTAC GGCCGCCTCT TCCAGAAACT CTGCTCCTCC 
AGCGCCGAGA TCCCCATCGG CATCTACAGG ACCGAGTGCC ATGTCTTCTC GGAGCCCCAT 
GACGTCAGAG CCCAGGGGGG CCCAGACATA GCCCTCCTGC CTGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGACTGCGA GGATACTCGG GAGGCCAAGG GACCCTGGGG CACACGAGCT 
GCATCTGGCA GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC AGTGGAGCAC 
CCACTACTAC GTCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGTCGCAA GAGCACCAAG 
CAGGCAGGGA AGGCACCTGT GGCCACAGAC TGGATCACCC AGCAGCGGCT CAGCCTGTAC 
CGACGCTCAG AGCGCCAGGA GCTCTCAGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGA 
CTGCCCACCA CTGGCTATGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AACGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAACCCA ACGACATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA TGTGGCCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAGCTCT CATCCTGTAA TCCTGAGACC AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497954.1
CDS	140..3790
Translation	MNDLDTEVLPLPPRYRFRDLLLGDQPFPNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497891.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497891.4

ORF Insert Sequence:

ATGAACGACC TGGACACGGA GGTGCTGCCC TTGCCGCCAA GGTACCGATT CCGTGACCTG 
CTTTTAGGAG ACCAGCCATT CCCGAACGAC GATAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GCAGGACTAC TACGTGGTCA TCCTGTGTCC CTCTGAAATG 
GACGTCCAGG TGCGCAGGGT GCTGCAGATT CCCCTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCTG CCCTCAAGGA CCAGGATCTC ATGCGAGCCA AGATGGACAA CGGAGAGGCC 
TGCTTTATCC TCAGCAGCAG GAATGAGGTG GACCGCACAG CTGCGGATCA CCAGACCATC 
CTTCGAGCCT GGGCTGTGAA GGACTTTGCC CCCAACTGTC CCCTCTATGT CCAGATCCTC 
AAGCCCGAAA ACAAGTTTCA CGTCAAATTT GCTGACCACG TGGTATGCGA GGAAGAGTGC 
AAGTACGCCA TGCTGGCCCT GAACTGCATC TGCCCGGCCA CCTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGTGG CCAGGAAGGA CAGGAGTCTC CCGAGCAGTG GCAGCGCACG 
TACGGGAGGT GCTCGGGCAA CGAGGTGTAC CACATTCGCA TGGGTGACAG TAAATTCTTC 
CGGGAGTATG AGGGCAAGAG CTTCACCTAC GCAGCCTTCC ACGCGCACAA GAAATATGGG 
GTGTGCCTCA TCGGGCTGAA GCGTGAGGAG AACAAGAGTA TCCTGCTGAA CCCAGGACCA 
CGGCACATCC TGGCTGCCTC CGACACCTGC TTCTATATCA ATATTACCAA GGAGGAGAAC 
TCAGCTTTCA TCTTCAAACA GGAGGAGAAG CAGAAGAGAC GGGGCCTTGC AGGGCAGGCA 
CTATATGAAG GGCCCTCCCG GCTCCCAGTG CATAGCATCA TCGCCTCTAT GGGGACAGTG 
GCCATGGACC TGCAGAACAC AGATTGCCGG CCCTCCCAGG GTGGCAGTGG CGGGGACGGC 
ACAAAGCTGA CTCTGCCCAC CGAGAACGGC TCTGGTAGTC GACGCCCCAG CATCGCACCC 
GTTCTGGAGT TGGCAGACAG CTCAGCCCTG TTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGATGAGG TGACACCCTC GGACGACGAG GGGCTCTCTG TGGTTGAGTA CGTGAAGGGC 
TATCCCCCCA ACTCACCCTA CATTGGCAGC TCCCCGACTT TATGCCACCT CCTGCCTGTG 
AAAGCCCCCT TCTGCTGCCT GCGGTTGGAC AAGGGCTGCA AACACAACAG CTACGAGGAT 
GCCAAGGCCT ATGGGTTCAA GAACAAGCTG ATTATTGTCT CTGCTGAGAC GGCAGGCAAC 
GGGCTCTACA ACTTCATCGT GCCTCTGCGC GCCTACTACC GGTCCCGCCG GGAGCTCAAC 
CCTATCGTGT TGCTGCTTGA CAACAAGCCT GACCACCACT TCCTGGAGGC CATCTGTTGC 
TTCCCCATGG TCTACTACAT GGAGGGATCC GTGGACAACC TGGACAGCTT ACTGCAGTGT 
GGCATCATCT ATGCTGACAA CCTGGTGGTG GTGGACAAGG AGAGCACTAT GAGCGCTGAA 
GAGGACTACA TGGCAGATGC CAAGACCATC GTCAATGTGC AAACCATGTT CCGGCTTTTC 
CCCAGTCTCA GCATCACCAC GGAGCTCACA CACCCTTCCA ACATGCGGTT CATGCAGTTC 
CGTGCCAAGG ACAGCTACTC TCTGGCTCTT TCCAAACTTG AAAAGCAAGA ACGGGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCATTTGCTG CTGGTCGAGT ATTTAGTATC 
AGCATGTTGG ATACACTGCT CTACCAGTCC TTCGTGAAGG ACTACATGAT CACCATCACC 
AGGCTGCTCT TGGGCCTTGA TACTACACCA GGCTCCGGCT ACCTCTGTGC AATGAAGGTA 
ACCGAGGACG ACCTGTGGAT CCGCACTTAC GGCCGCCTCT TCCAGAAACT CTGCTCCTCC 
AGCGCCGAGA TCCCCATCGG CATCTACAGG ACCGAGTGCC ATGTCTTCTC GGAGCCCCAT 
GACGTCAGAG CCCAGGGGGG CCCAGACATA GCCCTCCTGC CTGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGACTGCGA GGATACTCGG GAGGCCAAGG GACCCTGGGG CACACGAGCT 
GCATCTGGCA GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC AGTGGAGCAC 
CCACTACTAC GTCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGTCGCAA GAGCACCAAG 
CAGGCAGGGA AGGCACCTGT GGCCACAGAC TGGATCACCC AGCAGCGGCT CAGCCTGTAC 
CGACGCTCAG AGCGCCAGGA GCTCTCAGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGA 
CTGCCCACCA CTGGCTATGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AACGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAACCCA ACGACATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA TGTGGCCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAGCTCT CATCCTGTAA TCCTGAGACC AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497892.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497955.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X20, mRNA.

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CloneID	OMu29452
Clone ID Related Accession (Same CDS sequence)	XM_006497892.4 , XM_006497892.3
Accession Version	XM_006497892.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497892.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGACC TGGACACGGA GGTGCTGCCC TTGCCGCCAA GGTACCGATT CCGTGACCTG 
CTTTTAGGAG ACCAGCCATT CCCGAACGAC GATAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC 
GGGAGGTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG 
TGCCTCATCG GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG 
CACATCCTGG CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA 
TATGAAGGGC CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA 
AAGCTGACTC TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GATGAGGTGA CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT 
CCCCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC 
AAGGCCTATG GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT 
ATCGTGTTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGTTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTACT GCAGTGTGGC 
ATCATCTATG CTGACAACCT GGTGGTGGTG GACAAGGAGA GCACTATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATCGTC AATGTGCAAA CCATGTTCCG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACACAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGT 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGAGTATT TAGTATCAGC 
ATGTTGGATA CACTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTCTTGG GCCTTGATAC TACACCAGGC TCCGGCTACC TCTGTGCAAT GAAGGTAACC 
GAGGACGACC TGTGGATCCG CACTTACGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCCGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCGGA GCCCCATGAC 
GTCAGAGCCC AGTCTCAGAT CTCGGTGAAC ATGGAGGACT GCGAGGATAC TCGGGAGGCC 
AAGGGACCCT GGGGCACACG AGCTGCATCT GGCAGTGGCA GCACCCATGG CCGTCACGGG 
GGCAGTGCTG ACCCAGTGGA GCACCCACTA CTACGTCGCA AGAGCCTGCA GTGGGCCCGC 
AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA GGGAAGGCAC CTGTGGCCAC AGACTGGATC 
ACCCAGCAGC GGCTCAGCCT GTACCGACGC TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGAA TGAAGCACCT GGGACTGCCC ACCACTGGCT ATGATGAGAT GAACGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAACCC 
AACGACATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAGCTC TCATCCTGTA ATCCTGAGAC CAGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497955.1
CDS	178..3729
Translation	MNDLDTEVLPLPPRYRFRDLLLGDQPFPNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497892.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497892.4

ORF Insert Sequence:

ATGAACGACC TGGACACGGA GGTGCTGCCC TTGCCGCCAA GGTACCGATT CCGTGACCTG 
CTTTTAGGAG ACCAGCCATT CCCGAACGAC GATAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC 
GGGAGGTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG 
TGCCTCATCG GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG 
CACATCCTGG CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA 
TATGAAGGGC CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA 
AAGCTGACTC TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GATGAGGTGA CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT 
CCCCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC 
AAGGCCTATG GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT 
ATCGTGTTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGTTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTACT GCAGTGTGGC 
ATCATCTATG CTGACAACCT GGTGGTGGTG GACAAGGAGA GCACTATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATCGTC AATGTGCAAA CCATGTTCCG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACACAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGT 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGAGTATT TAGTATCAGC 
ATGTTGGATA CACTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTCTTGG GCCTTGATAC TACACCAGGC TCCGGCTACC TCTGTGCAAT GAAGGTAACC 
GAGGACGACC TGTGGATCCG CACTTACGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCCGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCGGA GCCCCATGAC 
GTCAGAGCCC AGTCTCAGAT CTCGGTGAAC ATGGAGGACT GCGAGGATAC TCGGGAGGCC 
AAGGGACCCT GGGGCACACG AGCTGCATCT GGCAGTGGCA GCACCCATGG CCGTCACGGG 
GGCAGTGCTG ACCCAGTGGA GCACCCACTA CTACGTCGCA AGAGCCTGCA GTGGGCCCGC 
AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA GGGAAGGCAC CTGTGGCCAC AGACTGGATC 
ACCCAGCAGC GGCTCAGCCT GTACCGACGC TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGAA TGAAGCACCT GGGACTGCCC ACCACTGGCT ATGATGAGAT GAACGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAACCC 
AACGACATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAGCTC TCATCCTGTA ATCCTGAGAC CAGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497893.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497956.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OMu29453
Clone ID Related Accession (Same CDS sequence)	XM_006497893.4 , XM_006497893.3
Accession Version	XM_006497893.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497893.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAACC AGGTGCTCAT CCTGTTCTGC ACCCTGCTGT GCCTGGTCTT CACAGGGACC 
TGTGGGATCC AGCACCTGGA GCGAGCAGGT GGCAACTTGA ACCTGCTGAC CTCCTTCTAC 
TTCTGCATCG TGACTTTCTC AACTGTGGGC TTCGGTGATG TGACGCCCAA GATCTGGCCA 
TCCCAGCTCC TGGTGGTCAT CCTGATCTGT GTCACCCTTG TGGTGCTCCC ACTGCAATTT 
GAAGAGCTTG TCTACCTCTG GATGGAGCGT CAGAAGTCAG GGGGCAACTA TAGCCGCCAC 
CGAGCACGGA CGGAGAAGCA CGTAGTCCTG TGTGTGAGCT CCCTCAAGAT TGATCTCCTC 
ATGGACTTCC TGAATGAGTT CTATGCCCAT CCCCGGCTCC AGCAGGACTA CTACGTGGTC 
ATCCTGTGTC CCTCTGAAAT GGACGTCCAG GTGCGCAGGG TGCTGCAGAT TCCCCTGTGG 
TCCCAGCGGG TCATCTACCT CCAGGGCTCT GCCCTCAAGG ACCAGGATCT CATGCGAGCC 
AAGATGGACA ACGGAGAGGC CTGCTTTATC CTCAGCAGCA GGAATGAGGT GGACCGCACA 
GCTGCGGATC ACCAGACCAT CCTTCGAGCC TGGGCTGTGA AGGACTTTGC CCCCAACTGT 
CCCCTCTATG TCCAGATCCT CAAGCCCGAA AACAAGTTTC ACGTCAAATT TGCTGACCAC 
GTGGTATGCG AGGAAGAGTG CAAGTACGCC ATGCTGGCCC TGAACTGCAT CTGCCCGGCC 
ACCTCCACCC TCATCACCCT GCTGGTGCAC ACGTCCCGTG GCCAGGAAGG ACAGGAGTCT 
CCCGAGCAGT GGCAGCGCAC GTACGGGAGG TGCTCGGGCA ACGAGGTGTA CCACATTCGC 
ATGGGTGACA GTAAATTCTT CCGGGAGTAT GAGGGCAAGA GCTTCACCTA CGCAGCCTTC 
CACGCGCACA AGAAATATGG GGTGTGCCTC ATCGGGCTGA AGCGTGAGGA GAACAAGAGT 
ATCCTGCTGA ACCCAGGACC ACGGCACATC CTGGCTGCCT CCGACACCTG CTTCTATATC 
AATATTACCA AGGAGGAGAA CTCAGCTTTC ATCTTCAAAC AGGAGGAGAA GCAGAAGAGA 
CGGGGCCTTG CAGGGCAGGC ACTATATGAA GGGCCCTCCC GGCTCCCAGT GCATAGCATC 
ATCGCCTCTA TGGGGACAGT GGCCATGGAC CTGCAGAACA CAGATTGCCG GCCCTCCCAG 
GGTGGCAGTG GCGGGGACGG CACAAAGCTG ACTCTGCCCA CCGAGAACGG CTCTGGTAGT 
CGACGCCCCA GCATCGCACC CGTTCTGGAG TTGGCAGACA GCTCAGCCCT GTTGCCCTGC 
GACCTGCTGA GTGACCAGTC AGAGGATGAG GTGACACCCT CGGACGACGA GGGGCTCTCT 
GTGGTTGAGT ACGTGAAGGG CTATCCCCCC AACTCACCCT ACATTGGCAG CTCCCCGACT 
TTATGCCACC TCCTGCCTGT GAAAGCCCCC TTCTGCTGCC TGCGGTTGGA CAAGGGCTGC 
AAACACAACA GCTACGAGGA TGCCAAGGCC TATGGGTTCA AGAACAAGCT GATTATTGTC 
TCTGCTGAGA CGGCAGGCAA CGGGCTCTAC AACTTCATCG TGCCTCTGCG CGCCTACTAC 
CGGTCCCGCC GGGAGCTCAA CCCTATCGTG TTGCTGCTTG ACAACAAGCC TGACCACCAC 
TTCCTGGAGG CCATCTGTTG CTTCCCCATG GTCTACTACA TGGAGGGATC CGTGGACAAC 
CTGGACAGCT TACTGCAGTG TGGCATCATC TATGCTGACA ACCTGGTGGT GGTGGACAAG 
GAGAGCACTA TGAGCGCTGA AGAGGACTAC ATGGCAGATG CCAAGACCAT CGTCAATGTG 
CAAACCATGT TCCGGCTTTT CCCCAGTCTC AGCATCACCA CGGAGCTCAC ACACCCTTCC 
AACATGCGGT TCATGCAGTT CCGTGCCAAG GACAGCTACT CTCTGGCTCT TTCCAAACTT 
GAAAAGCAAG AACGGGAGAA CGGCTCCAAC CTGGCCTTCA TGTTCCGCCT GCCATTTGCT 
GCTGGTCGAG TATTTAGTAT CAGCATGTTG GATACACTGC TCTACCAGTC CTTCGTGAAG 
GACTACATGA TCACCATCAC CAGGCTGCTC TTGGGCCTTG ATACTACACC AGGCTCCGGC 
TACCTCTGTG CAATGAAGGT AACCGAGGAC GACCTGTGGA TCCGCACTTA CGGCCGCCTC 
TTCCAGAAAC TCTGCTCCTC CAGCGCCGAG ATCCCCATCG GCATCTACAG GACCGAGTGC 
CATGTCTTCT CGGAGCCCCA TGACGTCAGA GCCCAGGGGG GCCCAGACAT AGCCCTCCTG 
CCTGCCCAGT CTCAGATCTC GGTGAACATG GAGGACTGCG AGGATACTCG GGAGGCCAAG 
GGACCCTGGG GCACACGAGC TGCATCTGGC AGTGGCAGCA CCCATGGCCG TCACGGGGGC 
AGTGCTGACC CAGTGGAGCA CCCACTACTA CGTCGCAAGA GCCTGCAGTG GGCCCGCAAG 
CTGAGTCGCA AGAGCACCAA GCAGGCAGGG AAGGCACCTG TGGCCACAGA CTGGATCACC 
CAGCAGCGGC TCAGCCTGTA CCGACGCTCA GAGCGCCAGG AGCTCTCAGA GCTGGTCAAG 
AACCGAATGA AGCACCTGGG ACTGCCCACC ACTGGCTATG AGGACGTAGC AAATTTAACA 
GCCAGTGATG TGATGAATCG GGTAAACCTG GGATATTTGC AAGATGAGAT GAACGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAACCC 
AACGACATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAGCTC TCATCCTGTA ATCCTGAGAC CAGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497956.1
CDS	2819..5830
Translation	MFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497893.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497893.4

ORF Insert Sequence:

ATGTTCAACC AGGTGCTCAT CCTGTTCTGC ACCCTGCTGT GCCTGGTCTT CACAGGGACC 
TGTGGGATCC AGCACCTGGA GCGAGCAGGT GGCAACTTGA ACCTGCTGAC CTCCTTCTAC 
TTCTGCATCG TGACTTTCTC AACTGTGGGC TTCGGTGATG TGACGCCCAA GATCTGGCCA 
TCCCAGCTCC TGGTGGTCAT CCTGATCTGT GTCACCCTTG TGGTGCTCCC ACTGCAATTT 
GAAGAGCTTG TCTACCTCTG GATGGAGCGT CAGAAGTCAG GGGGCAACTA TAGCCGCCAC 
CGAGCACGGA CGGAGAAGCA CGTAGTCCTG TGTGTGAGCT CCCTCAAGAT TGATCTCCTC 
ATGGACTTCC TGAATGAGTT CTATGCCCAT CCCCGGCTCC AGCAGGACTA CTACGTGGTC 
ATCCTGTGTC CCTCTGAAAT GGACGTCCAG GTGCGCAGGG TGCTGCAGAT TCCCCTGTGG 
TCCCAGCGGG TCATCTACCT CCAGGGCTCT GCCCTCAAGG ACCAGGATCT CATGCGAGCC 
AAGATGGACA ACGGAGAGGC CTGCTTTATC CTCAGCAGCA GGAATGAGGT GGACCGCACA 
GCTGCGGATC ACCAGACCAT CCTTCGAGCC TGGGCTGTGA AGGACTTTGC CCCCAACTGT 
CCCCTCTATG TCCAGATCCT CAAGCCCGAA AACAAGTTTC ACGTCAAATT TGCTGACCAC 
GTGGTATGCG AGGAAGAGTG CAAGTACGCC ATGCTGGCCC TGAACTGCAT CTGCCCGGCC 
ACCTCCACCC TCATCACCCT GCTGGTGCAC ACGTCCCGTG GCCAGGAAGG ACAGGAGTCT 
CCCGAGCAGT GGCAGCGCAC GTACGGGAGG TGCTCGGGCA ACGAGGTGTA CCACATTCGC 
ATGGGTGACA GTAAATTCTT CCGGGAGTAT GAGGGCAAGA GCTTCACCTA CGCAGCCTTC 
CACGCGCACA AGAAATATGG GGTGTGCCTC ATCGGGCTGA AGCGTGAGGA GAACAAGAGT 
ATCCTGCTGA ACCCAGGACC ACGGCACATC CTGGCTGCCT CCGACACCTG CTTCTATATC 
AATATTACCA AGGAGGAGAA CTCAGCTTTC ATCTTCAAAC AGGAGGAGAA GCAGAAGAGA 
CGGGGCCTTG CAGGGCAGGC ACTATATGAA GGGCCCTCCC GGCTCCCAGT GCATAGCATC 
ATCGCCTCTA TGGGGACAGT GGCCATGGAC CTGCAGAACA CAGATTGCCG GCCCTCCCAG 
GGTGGCAGTG GCGGGGACGG CACAAAGCTG ACTCTGCCCA CCGAGAACGG CTCTGGTAGT 
CGACGCCCCA GCATCGCACC CGTTCTGGAG TTGGCAGACA GCTCAGCCCT GTTGCCCTGC 
GACCTGCTGA GTGACCAGTC AGAGGATGAG GTGACACCCT CGGACGACGA GGGGCTCTCT 
GTGGTTGAGT ACGTGAAGGG CTATCCCCCC AACTCACCCT ACATTGGCAG CTCCCCGACT 
TTATGCCACC TCCTGCCTGT GAAAGCCCCC TTCTGCTGCC TGCGGTTGGA CAAGGGCTGC 
AAACACAACA GCTACGAGGA TGCCAAGGCC TATGGGTTCA AGAACAAGCT GATTATTGTC 
TCTGCTGAGA CGGCAGGCAA CGGGCTCTAC AACTTCATCG TGCCTCTGCG CGCCTACTAC 
CGGTCCCGCC GGGAGCTCAA CCCTATCGTG TTGCTGCTTG ACAACAAGCC TGACCACCAC 
TTCCTGGAGG CCATCTGTTG CTTCCCCATG GTCTACTACA TGGAGGGATC CGTGGACAAC 
CTGGACAGCT TACTGCAGTG TGGCATCATC TATGCTGACA ACCTGGTGGT GGTGGACAAG 
GAGAGCACTA TGAGCGCTGA AGAGGACTAC ATGGCAGATG CCAAGACCAT CGTCAATGTG 
CAAACCATGT TCCGGCTTTT CCCCAGTCTC AGCATCACCA CGGAGCTCAC ACACCCTTCC 
AACATGCGGT TCATGCAGTT CCGTGCCAAG GACAGCTACT CTCTGGCTCT TTCCAAACTT 
GAAAAGCAAG AACGGGAGAA CGGCTCCAAC CTGGCCTTCA TGTTCCGCCT GCCATTTGCT 
GCTGGTCGAG TATTTAGTAT CAGCATGTTG GATACACTGC TCTACCAGTC CTTCGTGAAG 
GACTACATGA TCACCATCAC CAGGCTGCTC TTGGGCCTTG ATACTACACC AGGCTCCGGC 
TACCTCTGTG CAATGAAGGT AACCGAGGAC GACCTGTGGA TCCGCACTTA CGGCCGCCTC 
TTCCAGAAAC TCTGCTCCTC CAGCGCCGAG ATCCCCATCG GCATCTACAG GACCGAGTGC 
CATGTCTTCT CGGAGCCCCA TGACGTCAGA GCCCAGGGGG GCCCAGACAT AGCCCTCCTG 
CCTGCCCAGT CTCAGATCTC GGTGAACATG GAGGACTGCG AGGATACTCG GGAGGCCAAG 
GGACCCTGGG GCACACGAGC TGCATCTGGC AGTGGCAGCA CCCATGGCCG TCACGGGGGC 
AGTGCTGACC CAGTGGAGCA CCCACTACTA CGTCGCAAGA GCCTGCAGTG GGCCCGCAAG 
CTGAGTCGCA AGAGCACCAA GCAGGCAGGG AAGGCACCTG TGGCCACAGA CTGGATCACC 
CAGCAGCGGC TCAGCCTGTA CCGACGCTCA GAGCGCCAGG AGCTCTCAGA GCTGGTCAAG 
AACCGAATGA AGCACCTGGG ACTGCCCACC ACTGGCTATG AGGACGTAGC AAATTTAACA 
GCCAGTGATG TGATGAATCG GGTAAACCTG GGATATTTGC AAGATGAGAT GAACGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAACCC 
AACGACATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAGCTC TCATCCTGTA ATCCTGAGAC CAGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497877.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497940.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73346
Clone ID Related Accession (Same CDS sequence)	XM_006497877.3 , XM_006497877.4
Accession Version	XM_006497877.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497877.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGT CCTTCGTGAA GGACTACATG ATCACCATCA CCAGGCTGCT CTTGGGCCTT 
GATACTACAC CAGGCTCCGG CTACCTCTGT GCAATGAAGG TAACCGAGGA CGACCTGTGG 
ATCCGCACTT ACGGCCGCCT CTTCCAGAAA CTCTGCTCCT CCAGCGCCGA GATCCCCATC 
GGCATCTACA GGACCGAGTG CCATGTCTTC TCGGAGCCCC ATGACGTCAG AGCCCAGGGG 
GGCCCAGACA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGACTGC 
GAGGATACTC GGGAGGCCAA GGGACCCTGG GGCACACGAG CTGCATCTGG CAGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCAGTGGAGC ACCCACTACT ACGTCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGTCGC AAGAGCACCA AGCAGGCAGG GAAGGCACCT 
GTGGCCACAG ACTGGATCAC CCAGCAGCGG CTCAGCCTGT ACCGACGCTC AGAGCGCCAG 
GAGCTCTCAG AGCTGGTCAA GAACCGAATG AAGCACCTGG GACTGCCCAC CACTGGCTAT 
GAGGACGTAG CAAATTTAAC AGCCAGTGAT GTGATGAATC GGGTAAACCT GGGATATTTG 
CAAGATGAGA TGAACGATCA TCACCAGAAC ACCCTTTCCT ATGTACTCAT CAACCCCCCG 
CCAGACACAA GACTGGAACC CAACGACATT GTGTACCTCA TCCGTTCCGA CCCCCTGGCC 
CATGTGGCCA GCAGCTCCCA GAGTCGGAAA AGCAGCTGCA GCAACAAGCT CTCATCCTGT 
AATCCTGAGA CCAGGGATGA GACCCAGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497940.2
CDS	58..3810
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497877.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497877.4

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGT CCTTCGTGAA GGACTACATG ATCACCATCA CCAGGCTGCT CTTGGGCCTT 
GATACTACAC CAGGCTCCGG CTACCTCTGT GCAATGAAGG TAACCGAGGA CGACCTGTGG 
ATCCGCACTT ACGGCCGCCT CTTCCAGAAA CTCTGCTCCT CCAGCGCCGA GATCCCCATC 
GGCATCTACA GGACCGAGTG CCATGTCTTC TCGGAGCCCC ATGACGTCAG AGCCCAGGGG 
GGCCCAGACA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGACTGC 
GAGGATACTC GGGAGGCCAA GGGACCCTGG GGCACACGAG CTGCATCTGG CAGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCAGTGGAGC ACCCACTACT ACGTCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGTCGC AAGAGCACCA AGCAGGCAGG GAAGGCACCT 
GTGGCCACAG ACTGGATCAC CCAGCAGCGG CTCAGCCTGT ACCGACGCTC AGAGCGCCAG 
GAGCTCTCAG AGCTGGTCAA GAACCGAATG AAGCACCTGG GACTGCCCAC CACTGGCTAT 
GAGGACGTAG CAAATTTAAC AGCCAGTGAT GTGATGAATC GGGTAAACCT GGGATATTTG 
CAAGATGAGA TGAACGATCA TCACCAGAAC ACCCTTTCCT ATGTACTCAT CAACCCCCCG 
CCAGACACAA GACTGGAACC CAACGACATT GTGTACCTCA TCCGTTCCGA CCCCCTGGCC 
CATGTGGCCA GCAGCTCCCA GAGTCGGAAA AGCAGCTGCA GCAACAAGCT CTCATCCTGT 
AATCCTGAGA CCAGGGATGA GACCCAGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497878.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497941.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X2, mRNA.

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CloneID	OMu73347
Clone ID Related Accession (Same CDS sequence)	XM_006497878.3 , XM_006497878.4
Accession Version	XM_006497878.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497878.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGTTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTA CTGCAGTGTG GCATCATCTA TGCTGACAAC 
CTGGTGGTGG TGGACAAGGA GAGCACTATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATCG TCAATGTGCA AACCATGTTC CGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACAC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GTGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGAGTA TTTAGTATCA GCATGTTGGA TACACTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTCTT GGGCCTTGAT 
ACTACACCAG GCTCCGGCTA CCTCTGTGCA ATGAAGGTAA CCGAGGACGA CCTGTGGATC 
CGCACTTACG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCCGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCG GAGCCCCATG ACGTCAGAGC CCAGGGGGGC 
CCAGACATAG CCCTCCTGCC TGCCCAGTCT CAGATCTCGG TGAACATGGA GGACTGCGAG 
GATACTCGGG AGGCCAAGGG ACCCTGGGGC ACACGAGCTG CATCTGGCAG TGGCAGCACC 
CATGGCCGTC ACGGGGGCAG TGCTGACCCA GTGGAGCACC CACTACTACG TCGCAAGAGC 
CTGCAGTGGG CCCGCAAGCT GAGTCGCAAG AGCACCAAGC AGGCAGGGAA GGCACCTGTG 
GCCACAGACT GGATCACCCA GCAGCGGCTC AGCCTGTACC GACGCTCAGA GCGCCAGGAG 
CTCTCAGAGC TGGTCAAGAA CCGAATGAAG CACCTGGGAC TGCCCACCAC TGGCTATGAG 
GACGTAGCAA ATTTAACAGC CAGTGATGTG ATGAATCGGG TAAACCTGGG ATATTTGCAA 
GATGAGATGA ACGATCATCA CCAGAACACC CTTTCCTATG TACTCATCAA CCCCCCGCCA 
GACACAAGAC TGGAACCCAA CGACATTGTG TACCTCATCC GTTCCGACCC CCTGGCCCAT 
GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT 
CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497941.2
CDS	57..3806
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497878.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497878.4

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGTTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTA CTGCAGTGTG GCATCATCTA TGCTGACAAC 
CTGGTGGTGG TGGACAAGGA GAGCACTATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATCG TCAATGTGCA AACCATGTTC CGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACAC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GTGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGAGTA TTTAGTATCA GCATGTTGGA TACACTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTCTT GGGCCTTGAT 
ACTACACCAG GCTCCGGCTA CCTCTGTGCA ATGAAGGTAA CCGAGGACGA CCTGTGGATC 
CGCACTTACG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCCGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCG GAGCCCCATG ACGTCAGAGC CCAGGGGGGC 
CCAGACATAG CCCTCCTGCC TGCCCAGTCT CAGATCTCGG TGAACATGGA GGACTGCGAG 
GATACTCGGG AGGCCAAGGG ACCCTGGGGC ACACGAGCTG CATCTGGCAG TGGCAGCACC 
CATGGCCGTC ACGGGGGCAG TGCTGACCCA GTGGAGCACC CACTACTACG TCGCAAGAGC 
CTGCAGTGGG CCCGCAAGCT GAGTCGCAAG AGCACCAAGC AGGCAGGGAA GGCACCTGTG 
GCCACAGACT GGATCACCCA GCAGCGGCTC AGCCTGTACC GACGCTCAGA GCGCCAGGAG 
CTCTCAGAGC TGGTCAAGAA CCGAATGAAG CACCTGGGAC TGCCCACCAC TGGCTATGAG 
GACGTAGCAA ATTTAACAGC CAGTGATGTG ATGAATCGGG TAAACCTGGG ATATTTGCAA 
GATGAGATGA ACGATCATCA CCAGAACACC CTTTCCTATG TACTCATCAA CCCCCCGCCA 
GACACAAGAC TGGAACCCAA CGACATTGTG TACCTCATCC GTTCCGACCC CCTGGCCCAT 
GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT 
CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497879.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497942.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu73348
Clone ID Related Accession (Same CDS sequence)	XM_006497879.4 , XM_006497879.3
Accession Version	XM_006497879.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497879.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGTGGCCA TGGACCTGCA GAACACAGAT 
TGCCGGCCCT CCCAGGGTGG CAGTGGCGGG GACGGCACAA AGCTGACTCT GCCCACCGAG 
AACGGCTCTG GTAGTCGACG CCCCAGCATC GCACCCGTTC TGGAGTTGGC AGACAGCTCA 
GCCCTGTTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ATGAGGTGAC ACCCTCGGAC 
GACGAGGGGC TCTCTGTGGT TGAGTACGTG AAGGGCTATC CCCCCAACTC ACCCTACATT 
GGCAGCTCCC CGACTTTATG CCACCTCCTG CCTGTGAAAG CCCCCTTCTG CTGCCTGCGG 
TTGGACAAGG GCTGCAAACA CAACAGCTAC GAGGATGCCA AGGCCTATGG GTTCAAGAAC 
AAGCTGATTA TTGTCTCTGC TGAGACGGCA GGCAACGGGC TCTACAACTT CATCGTGCCT 
CTGCGCGCCT ACTACCGGTC CCGCCGGGAG CTCAACCCTA TCGTGTTGCT GCTTGACAAC 
AAGCCTGACC ACCACTTCCT GGAGGCCATC TGTTGCTTCC CCATGGTCTA CTACATGGAG 
GGATCCGTGG ACAACCTGGA CAGCTTACTG CAGTGTGGCA TCATCTATGC TGACAACCTG 
GTGGTGGTGG ACAAGGAGAG CACTATGAGC GCTGAAGAGG ACTACATGGC AGATGCCAAG 
ACCATCGTCA ATGTGCAAAC CATGTTCCGG CTTTTCCCCA GTCTCAGCAT CACCACGGAG 
CTCACACACC CTTCCAACAT GCGGTTCATG CAGTTCCGTG CCAAGGACAG CTACTCTCTG 
GCTCTTTCCA AACTTGAAAA GCAAGAACGG GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCAT TTGCTGCTGG TCGAGTATTT AGTATCAGCA TGTTGGATAC ACTGCTCTAC 
CAGTCCTTCG TGAAGGACTA CATGATCACC ATCACCAGGC TGCTCTTGGG CCTTGATACT 
ACACCAGGCT CCGGCTACCT CTGTGCAATG AAGGTAACCG AGGACGACCT GTGGATCCGC 
ACTTACGGCC GCCTCTTCCA GAAACTCTGC TCCTCCAGCG CCGAGATCCC CATCGGCATC 
TACAGGACCG AGTGCCATGT CTTCTCGGAG CCCCATGACG TCAGAGCCCA GGGGGGCCCA 
GACATAGCCC TCCTGCCTGC CCAGTCTCAG ATCTCGGTGA ACATGGAGGA CTGCGAGGAT 
ACTCGGGAGG CCAAGGGACC CTGGGGCACA CGAGCTGCAT CTGGCAGTGG CAGCACCCAT 
GGCCGTCACG GGGGCAGTGC TGACCCAGTG GAGCACCCAC TACTACGTCG CAAGAGCCTG 
CAGTGGGCCC GCAAGCTGAG TCGCAAGAGC ACCAAGCAGG CAGGGAAGGC ACCTGTGGCC 
ACAGACTGGA TCACCCAGCA GCGGCTCAGC CTGTACCGAC GCTCAGAGCG CCAGGAGCTC 
TCAGAGCTGG TCAAGAACCG AATGAAGCAC CTGGGACTGC CCACCACTGG CTATGAGGAC 
GTAGCAAATT TAACAGCCAG TGATGTGATG AATCGGGTAA ACCTGGGATA TTTGCAAGAT 
GAGATGAACG ATCATCACCA GAACACCCTT TCCTATGTAC TCATCAACCC CCCGCCAGAC 
ACAAGACTGG AACCCAACGA CATTGTGTAC CTCATCCGTT CCGACCCCCT GGCCCATGTG 
GCCAGCAGCT CCCAGAGTCG GAAAAGCAGC TGCAGCAACA AGCTCTCATC CTGTAATCCT 
GAGACCAGGG ATGAGACCCA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497942.2
CDS	59..3805
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497879.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497879.4

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGTGGCCA TGGACCTGCA GAACACAGAT 
TGCCGGCCCT CCCAGGGTGG CAGTGGCGGG GACGGCACAA AGCTGACTCT GCCCACCGAG 
AACGGCTCTG GTAGTCGACG CCCCAGCATC GCACCCGTTC TGGAGTTGGC AGACAGCTCA 
GCCCTGTTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ATGAGGTGAC ACCCTCGGAC 
GACGAGGGGC TCTCTGTGGT TGAGTACGTG AAGGGCTATC CCCCCAACTC ACCCTACATT 
GGCAGCTCCC CGACTTTATG CCACCTCCTG CCTGTGAAAG CCCCCTTCTG CTGCCTGCGG 
TTGGACAAGG GCTGCAAACA CAACAGCTAC GAGGATGCCA AGGCCTATGG GTTCAAGAAC 
AAGCTGATTA TTGTCTCTGC TGAGACGGCA GGCAACGGGC TCTACAACTT CATCGTGCCT 
CTGCGCGCCT ACTACCGGTC CCGCCGGGAG CTCAACCCTA TCGTGTTGCT GCTTGACAAC 
AAGCCTGACC ACCACTTCCT GGAGGCCATC TGTTGCTTCC CCATGGTCTA CTACATGGAG 
GGATCCGTGG ACAACCTGGA CAGCTTACTG CAGTGTGGCA TCATCTATGC TGACAACCTG 
GTGGTGGTGG ACAAGGAGAG CACTATGAGC GCTGAAGAGG ACTACATGGC AGATGCCAAG 
ACCATCGTCA ATGTGCAAAC CATGTTCCGG CTTTTCCCCA GTCTCAGCAT CACCACGGAG 
CTCACACACC CTTCCAACAT GCGGTTCATG CAGTTCCGTG CCAAGGACAG CTACTCTCTG 
GCTCTTTCCA AACTTGAAAA GCAAGAACGG GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCAT TTGCTGCTGG TCGAGTATTT AGTATCAGCA TGTTGGATAC ACTGCTCTAC 
CAGTCCTTCG TGAAGGACTA CATGATCACC ATCACCAGGC TGCTCTTGGG CCTTGATACT 
ACACCAGGCT CCGGCTACCT CTGTGCAATG AAGGTAACCG AGGACGACCT GTGGATCCGC 
ACTTACGGCC GCCTCTTCCA GAAACTCTGC TCCTCCAGCG CCGAGATCCC CATCGGCATC 
TACAGGACCG AGTGCCATGT CTTCTCGGAG CCCCATGACG TCAGAGCCCA GGGGGGCCCA 
GACATAGCCC TCCTGCCTGC CCAGTCTCAG ATCTCGGTGA ACATGGAGGA CTGCGAGGAT 
ACTCGGGAGG CCAAGGGACC CTGGGGCACA CGAGCTGCAT CTGGCAGTGG CAGCACCCAT 
GGCCGTCACG GGGGCAGTGC TGACCCAGTG GAGCACCCAC TACTACGTCG CAAGAGCCTG 
CAGTGGGCCC GCAAGCTGAG TCGCAAGAGC ACCAAGCAGG CAGGGAAGGC ACCTGTGGCC 
ACAGACTGGA TCACCCAGCA GCGGCTCAGC CTGTACCGAC GCTCAGAGCG CCAGGAGCTC 
TCAGAGCTGG TCAAGAACCG AATGAAGCAC CTGGGACTGC CCACCACTGG CTATGAGGAC 
GTAGCAAATT TAACAGCCAG TGATGTGATG AATCGGGTAA ACCTGGGATA TTTGCAAGAT 
GAGATGAACG ATCATCACCA GAACACCCTT TCCTATGTAC TCATCAACCC CCCGCCAGAC 
ACAAGACTGG AACCCAACGA CATTGTGTAC CTCATCCGTT CCGACCCCCT GGCCCATGTG 
GCCAGCAGCT CCCAGAGTCG GAAAAGCAGC TGCAGCAACA AGCTCTCATC CTGTAATCCT 
GAGACCAGGG ATGAGACCCA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497880.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497943.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu73349
Clone ID Related Accession (Same CDS sequence)	XM_006497880.4 , XM_006497880.3
Accession Version	XM_006497880.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497880.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGT CCTTCGTGAA GGACTACATG ATCACCATCA CCAGGCTGCT CTTGGGCCTT 
GATACTACAC CAGGCTCCGG CTACCTCTGT GCAATGAAGG TAACCGAGGA CGACCTGTGG 
ATCCGCACTT ACGGCCGCCT CTTCCAGAAA CTCTGCTCCT CCAGCGCCGA GATCCCCATC 
GGCATCTACA GGACCGAGTG CCATGTCTTC TCGGAGCCCC ATGACGTCAG AGCCCAGTCT 
CAGATCTCGG TGAACATGGA GGACTGCGAG GATACTCGGG AGGCCAAGGG ACCCTGGGGC 
ACACGAGCTG CATCTGGCAG TGGCAGCACC CATGGCCGTC ACGGGGGCAG TGCTGACCCA 
GTGGAGCACC CACTACTACG TCGCAAGAGC CTGCAGTGGG CCCGCAAGCT GAGTCGCAAG 
AGCACCAAGC AGGCAGGGAA GGCACCTGTG GCCACAGACT GGATCACCCA GCAGCGGCTC 
AGCCTGTACC GACGCTCAGA GCGCCAGGAG CTCTCAGAGC TGGTCAAGAA CCGAATGAAG 
CACCTGGGAC TGCCCACCAC TGGCTATGAG GACGTAGCAA ATTTAACAGC CAGTGATGTG 
ATGAATCGGG TAAACCTGGG ATATTTGCAA GATGAGATGA ACGATCATCA CCAGAACACC 
CTTTCCTATG TACTCATCAA CCCCCCGCCA GACACAAGAC TGGAACCCAA CGACATTGTG 
TACCTCATCC GTTCCGACCC CCTGGCCCAT GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC 
AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497943.2
CDS	57..3776
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497880.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497880.4

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGT CCTTCGTGAA GGACTACATG ATCACCATCA CCAGGCTGCT CTTGGGCCTT 
GATACTACAC CAGGCTCCGG CTACCTCTGT GCAATGAAGG TAACCGAGGA CGACCTGTGG 
ATCCGCACTT ACGGCCGCCT CTTCCAGAAA CTCTGCTCCT CCAGCGCCGA GATCCCCATC 
GGCATCTACA GGACCGAGTG CCATGTCTTC TCGGAGCCCC ATGACGTCAG AGCCCAGTCT 
CAGATCTCGG TGAACATGGA GGACTGCGAG GATACTCGGG AGGCCAAGGG ACCCTGGGGC 
ACACGAGCTG CATCTGGCAG TGGCAGCACC CATGGCCGTC ACGGGGGCAG TGCTGACCCA 
GTGGAGCACC CACTACTACG TCGCAAGAGC CTGCAGTGGG CCCGCAAGCT GAGTCGCAAG 
AGCACCAAGC AGGCAGGGAA GGCACCTGTG GCCACAGACT GGATCACCCA GCAGCGGCTC 
AGCCTGTACC GACGCTCAGA GCGCCAGGAG CTCTCAGAGC TGGTCAAGAA CCGAATGAAG 
CACCTGGGAC TGCCCACCAC TGGCTATGAG GACGTAGCAA ATTTAACAGC CAGTGATGTG 
ATGAATCGGG TAAACCTGGG ATATTTGCAA GATGAGATGA ACGATCATCA CCAGAACACC 
CTTTCCTATG TACTCATCAA CCCCCCGCCA GACACAAGAC TGGAACCCAA CGACATTGTG 
TACCTCATCC GTTCCGACCC CCTGGCCCAT GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC 
AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497881.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497944.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X5, mRNA.

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CloneID	OMu73350
Clone ID Related Accession (Same CDS sequence)	XM_006497881.4 , XM_006497881.3
Accession Version	XM_006497881.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497881.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GTGGCCATGG ACCTGCAGAA CACAGATTGC 
CGGCCCTCCC AGGGTGGCAG TGGCGGGGAC GGCACAAAGC TGACTCTGCC CACCGAGAAC 
GGCTCTGGTA GTCGACGCCC CAGCATCGCA CCCGTTCTGG AGTTGGCAGA CAGCTCAGCC 
CTGTTGCCCT GCGACCTGCT GAGTGACCAG TCAGAGGATG AGGTGACACC CTCGGACGAC 
GAGGGGCTCT CTGTGGTTGA GTACGTGAAG GGCTATCCCC CCAACTCACC CTACATTGGC 
AGCTCCCCGA CTTTATGCCA CCTCCTGCCT GTGAAAGCCC CCTTCTGCTG CCTGCGGTTG 
GACAAGGGCT GCAAACACAA CAGCTACGAG GATGCCAAGG CCTATGGGTT CAAGAACAAG 
CTGATTATTG TCTCTGCTGA GACGGCAGGC AACGGGCTCT ACAACTTCAT CGTGCCTCTG 
CGCGCCTACT ACCGGTCCCG CCGGGAGCTC AACCCTATCG TGTTGCTGCT TGACAACAAG 
CCTGACCACC ACTTCCTGGA GGCCATCTGT TGCTTCCCCA TGGTCTACTA CATGGAGGGA 
TCCGTGGACA ACCTGGACAG CTTACTGCAG TGTGGCATCA TCTATGCTGA CAACCTGGTG 
GTGGTGGACA AGGAGAGCAC TATGAGCGCT GAAGAGGACT ACATGGCAGA TGCCAAGACC 
ATCGTCAATG TGCAAACCAT GTTCCGGCTT TTCCCCAGTC TCAGCATCAC CACGGAGCTC 
ACACACCCTT CCAACATGCG GTTCATGCAG TTCCGTGCCA AGGACAGCTA CTCTCTGGCT 
CTTTCCAAAC TTGAAAAGCA AGAACGGGAG AACGGCTCCA ACCTGGCCTT CATGTTCCGC 
CTGCCATTTG CTGCTGGTCG AGTATTTAGT ATCAGCATGT TGGATACACT GCTCTACCAG 
TCCTTCGTGA AGGACTACAT GATCACCATC ACCAGGCTGC TCTTGGGCCT TGATACTACA 
CCAGGCTCCG GCTACCTCTG TGCAATGAAG GTAACCGAGG ACGACCTGTG GATCCGCACT 
TACGGCCGCC TCTTCCAGAA ACTCTGCTCC TCCAGCGCCG AGATCCCCAT CGGCATCTAC 
AGGACCGAGT GCCATGTCTT CTCGGAGCCC CATGACGTCA GAGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGACTGCGA GGATACTCGG GAGGCCAAGG GACCCTGGGG CACACGAGCT 
GCATCTGGCA GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC AGTGGAGCAC 
CCACTACTAC GTCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGTCGCAA GAGCACCAAG 
CAGGCAGGGA AGGCACCTGT GGCCACAGAC TGGATCACCC AGCAGCGGCT CAGCCTGTAC 
CGACGCTCAG AGCGCCAGGA GCTCTCAGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGA 
CTGCCCACCA CTGGCTATGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AACGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAACCCA ACGACATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA TGTGGCCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAGCTCT CATCCTGTAA TCCTGAGACC AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497944.2
CDS	57..3767
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497881.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497881.4

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GTGGCCATGG ACCTGCAGAA CACAGATTGC 
CGGCCCTCCC AGGGTGGCAG TGGCGGGGAC GGCACAAAGC TGACTCTGCC CACCGAGAAC 
GGCTCTGGTA GTCGACGCCC CAGCATCGCA CCCGTTCTGG AGTTGGCAGA CAGCTCAGCC 
CTGTTGCCCT GCGACCTGCT GAGTGACCAG TCAGAGGATG AGGTGACACC CTCGGACGAC 
GAGGGGCTCT CTGTGGTTGA GTACGTGAAG GGCTATCCCC CCAACTCACC CTACATTGGC 
AGCTCCCCGA CTTTATGCCA CCTCCTGCCT GTGAAAGCCC CCTTCTGCTG CCTGCGGTTG 
GACAAGGGCT GCAAACACAA CAGCTACGAG GATGCCAAGG CCTATGGGTT CAAGAACAAG 
CTGATTATTG TCTCTGCTGA GACGGCAGGC AACGGGCTCT ACAACTTCAT CGTGCCTCTG 
CGCGCCTACT ACCGGTCCCG CCGGGAGCTC AACCCTATCG TGTTGCTGCT TGACAACAAG 
CCTGACCACC ACTTCCTGGA GGCCATCTGT TGCTTCCCCA TGGTCTACTA CATGGAGGGA 
TCCGTGGACA ACCTGGACAG CTTACTGCAG TGTGGCATCA TCTATGCTGA CAACCTGGTG 
GTGGTGGACA AGGAGAGCAC TATGAGCGCT GAAGAGGACT ACATGGCAGA TGCCAAGACC 
ATCGTCAATG TGCAAACCAT GTTCCGGCTT TTCCCCAGTC TCAGCATCAC CACGGAGCTC 
ACACACCCTT CCAACATGCG GTTCATGCAG TTCCGTGCCA AGGACAGCTA CTCTCTGGCT 
CTTTCCAAAC TTGAAAAGCA AGAACGGGAG AACGGCTCCA ACCTGGCCTT CATGTTCCGC 
CTGCCATTTG CTGCTGGTCG AGTATTTAGT ATCAGCATGT TGGATACACT GCTCTACCAG 
TCCTTCGTGA AGGACTACAT GATCACCATC ACCAGGCTGC TCTTGGGCCT TGATACTACA 
CCAGGCTCCG GCTACCTCTG TGCAATGAAG GTAACCGAGG ACGACCTGTG GATCCGCACT 
TACGGCCGCC TCTTCCAGAA ACTCTGCTCC TCCAGCGCCG AGATCCCCAT CGGCATCTAC 
AGGACCGAGT GCCATGTCTT CTCGGAGCCC CATGACGTCA GAGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGACTGCGA GGATACTCGG GAGGCCAAGG GACCCTGGGG CACACGAGCT 
GCATCTGGCA GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC AGTGGAGCAC 
CCACTACTAC GTCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGTCGCAA GAGCACCAAG 
CAGGCAGGGA AGGCACCTGT GGCCACAGAC TGGATCACCC AGCAGCGGCT CAGCCTGTAC 
CGACGCTCAG AGCGCCAGGA GCTCTCAGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGA 
CTGCCCACCA CTGGCTATGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AACGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAACCCA ACGACATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA TGTGGCCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAGCTCT CATCCTGTAA TCCTGAGACC AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497882.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497945.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu73351
Clone ID Related Accession (Same CDS sequence)	XM_006497882.3 , XM_006497882.4
Accession Version	XM_006497882.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497882.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GCAGGACTAC TACGTGGTCA TCCTGTGTCC CTCTGAAATG 
GACGTCCAGG TGCGCAGGGT GCTGCAGATT CCCCTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCTG CCCTCAAGGA CCAGGATCTC ATGCGAGCCA AGATGGACAA CGGAGAGGCC 
TGCTTTATCC TCAGCAGCAG GAATGAGGTG GACCGCACAG CTGCGGATCA CCAGACCATC 
CTTCGAGCCT GGGCTGTGAA GGACTTTGCC CCCAACTGTC CCCTCTATGT CCAGATCCTC 
AAGCCCGAAA ACAAGTTTCA CGTCAAATTT GCTGACCACG TGGTATGCGA GGAAGAGTGC 
AAGTACGCCA TGCTGGCCCT GAACTGCATC TGCCCGGCCA CCTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGTGG CCAGGAAGGA CAGGAGTCTC CCGAGCAGTG GCAGCGCACG 
TACGGGAGGT GCTCGGGCAA CGAGGTGTAC CACATTCGCA TGGGTGACAG TAAATTCTTC 
CGGGAGTATG AGGGCAAGAG CTTCACCTAC GCAGCCTTCC ACGCGCACAA GAAATATGGG 
GTGTGCCTCA TCGGGCTGAA GCGTGAGGAG AACAAGAGTA TCCTGCTGAA CCCAGGACCA 
CGGCACATCC TGGCTGCCTC CGACACCTGC TTCTATATCA ATATTACCAA GGAGGAGAAC 
TCAGCTTTCA TCTTCAAACA GGAGGAGAAG CAGAAGAGAC GGGGCCTTGC AGGGCAGGCA 
CTATATGAAG GGCCCTCCCG GCTCCCAGTG CATAGCATCA TCGCCTCTAT GGGGACAGTG 
GCCATGGACC TGCAGAACAC AGATTGCCGG CCCTCCCAGG GTGGCAGTGG CGGGGACGGC 
ACAAAGCTGA CTCTGCCCAC CGAGAACGGC TCTGGTAGTC GACGCCCCAG CATCGCACCC 
GTTCTGGAGT TGGCAGACAG CTCAGCCCTG TTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGATGAGG TGACACCCTC GGACGACGAG GGGCTCTCTG TGGTTGAGTA CGTGAAGGGC 
TATCCCCCCA ACTCACCCTA CATTGGCAGC TCCCCGACTT TATGCCACCT CCTGCCTGTG 
AAAGCCCCCT TCTGCTGCCT GCGGTTGGAC AAGGGCTGCA AACACAACAG CTACGAGGAT 
GCCAAGGCCT ATGGGTTCAA GAACAAGCTG ATTATTGTCT CTGCTGAGAC GGCAGGCAAC 
GGGCTCTACA ACTTCATCGT GCCTCTGCGC GCCTACTACC GGTCCCGCCG GGAGCTCAAC 
CCTATCGTGT TGCTGCTTGA CAACAAGCCT GACCACCACT TCCTGGAGGC CATCTGTTGC 
TTCCCCATGG TCTACTACAT GGAGGGATCC GTGGACAACC TGGACAGCTT ACTGCAGTGT 
GGCATCATCT ATGCTGACAA CCTGGTGGTG GTGGACAAGG AGAGCACTAT GAGCGCTGAA 
GAGGACTACA TGGCAGATGC CAAGACCATC GTCAATGTGC AAACCATGTT CCGGCTTTTC 
CCCAGTCTCA GCATCACCAC GGAGCTCACA CACCCTTCCA ACATGCGGTT CATGCAGTTC 
CGTGCCAAGG ACAGCTACTC TCTGGCTCTT TCCAAACTTG AAAAGCAAGA ACGGGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCATTTGCTG CTGGTCGAGT ATTTAGTATC 
AGCATGTTGG ATACACTGCT CTACCAGTCC TTCGTGAAGG ACTACATGAT CACCATCACC 
AGGCTGCTCT TGGGCCTTGA TACTACACCA GGCTCCGGCT ACCTCTGTGC AATGAAGGTA 
ACCGAGGACG ACCTGTGGAT CCGCACTTAC GGCCGCCTCT TCCAGAAACT CTGCTCCTCC 
AGCGCCGAGA TCCCCATCGG CATCTACAGG ACCGAGTGCC ATGTCTTCTC GGAGCCCCAT 
GACGTCAGAG CCCAGGGGGG CCCAGACATA GCCCTCCTGC CTGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGACTGCGA GGATACTCGG GAGGCCAAGG GACCCTGGGG CACACGAGCT 
GCATCTGGCA GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC AGTGGAGCAC 
CCACTACTAC GTCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGTCGCAA GAGCACCAAG 
CAGGCAGGGA AGGCACCTGT GGCCACAGAC TGGATCACCC AGCAGCGGCT CAGCCTGTAC 
CGACGCTCAG AGCGCCAGGA GCTCTCAGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGA 
CTGCCCACCA CTGGCTATGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AACGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAACCCA ACGACATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA TGTGGCCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAGCTCT CATCCTGTAA TCCTGAGACC AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497945.2
CDS	58..3768
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497882.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497882.4

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GCAGGACTAC TACGTGGTCA TCCTGTGTCC CTCTGAAATG 
GACGTCCAGG TGCGCAGGGT GCTGCAGATT CCCCTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCTG CCCTCAAGGA CCAGGATCTC ATGCGAGCCA AGATGGACAA CGGAGAGGCC 
TGCTTTATCC TCAGCAGCAG GAATGAGGTG GACCGCACAG CTGCGGATCA CCAGACCATC 
CTTCGAGCCT GGGCTGTGAA GGACTTTGCC CCCAACTGTC CCCTCTATGT CCAGATCCTC 
AAGCCCGAAA ACAAGTTTCA CGTCAAATTT GCTGACCACG TGGTATGCGA GGAAGAGTGC 
AAGTACGCCA TGCTGGCCCT GAACTGCATC TGCCCGGCCA CCTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGTGG CCAGGAAGGA CAGGAGTCTC CCGAGCAGTG GCAGCGCACG 
TACGGGAGGT GCTCGGGCAA CGAGGTGTAC CACATTCGCA TGGGTGACAG TAAATTCTTC 
CGGGAGTATG AGGGCAAGAG CTTCACCTAC GCAGCCTTCC ACGCGCACAA GAAATATGGG 
GTGTGCCTCA TCGGGCTGAA GCGTGAGGAG AACAAGAGTA TCCTGCTGAA CCCAGGACCA 
CGGCACATCC TGGCTGCCTC CGACACCTGC TTCTATATCA ATATTACCAA GGAGGAGAAC 
TCAGCTTTCA TCTTCAAACA GGAGGAGAAG CAGAAGAGAC GGGGCCTTGC AGGGCAGGCA 
CTATATGAAG GGCCCTCCCG GCTCCCAGTG CATAGCATCA TCGCCTCTAT GGGGACAGTG 
GCCATGGACC TGCAGAACAC AGATTGCCGG CCCTCCCAGG GTGGCAGTGG CGGGGACGGC 
ACAAAGCTGA CTCTGCCCAC CGAGAACGGC TCTGGTAGTC GACGCCCCAG CATCGCACCC 
GTTCTGGAGT TGGCAGACAG CTCAGCCCTG TTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGATGAGG TGACACCCTC GGACGACGAG GGGCTCTCTG TGGTTGAGTA CGTGAAGGGC 
TATCCCCCCA ACTCACCCTA CATTGGCAGC TCCCCGACTT TATGCCACCT CCTGCCTGTG 
AAAGCCCCCT TCTGCTGCCT GCGGTTGGAC AAGGGCTGCA AACACAACAG CTACGAGGAT 
GCCAAGGCCT ATGGGTTCAA GAACAAGCTG ATTATTGTCT CTGCTGAGAC GGCAGGCAAC 
GGGCTCTACA ACTTCATCGT GCCTCTGCGC GCCTACTACC GGTCCCGCCG GGAGCTCAAC 
CCTATCGTGT TGCTGCTTGA CAACAAGCCT GACCACCACT TCCTGGAGGC CATCTGTTGC 
TTCCCCATGG TCTACTACAT GGAGGGATCC GTGGACAACC TGGACAGCTT ACTGCAGTGT 
GGCATCATCT ATGCTGACAA CCTGGTGGTG GTGGACAAGG AGAGCACTAT GAGCGCTGAA 
GAGGACTACA TGGCAGATGC CAAGACCATC GTCAATGTGC AAACCATGTT CCGGCTTTTC 
CCCAGTCTCA GCATCACCAC GGAGCTCACA CACCCTTCCA ACATGCGGTT CATGCAGTTC 
CGTGCCAAGG ACAGCTACTC TCTGGCTCTT TCCAAACTTG AAAAGCAAGA ACGGGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCATTTGCTG CTGGTCGAGT ATTTAGTATC 
AGCATGTTGG ATACACTGCT CTACCAGTCC TTCGTGAAGG ACTACATGAT CACCATCACC 
AGGCTGCTCT TGGGCCTTGA TACTACACCA GGCTCCGGCT ACCTCTGTGC AATGAAGGTA 
ACCGAGGACG ACCTGTGGAT CCGCACTTAC GGCCGCCTCT TCCAGAAACT CTGCTCCTCC 
AGCGCCGAGA TCCCCATCGG CATCTACAGG ACCGAGTGCC ATGTCTTCTC GGAGCCCCAT 
GACGTCAGAG CCCAGGGGGG CCCAGACATA GCCCTCCTGC CTGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGACTGCGA GGATACTCGG GAGGCCAAGG GACCCTGGGG CACACGAGCT 
GCATCTGGCA GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC AGTGGAGCAC 
CCACTACTAC GTCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGTCGCAA GAGCACCAAG 
CAGGCAGGGA AGGCACCTGT GGCCACAGAC TGGATCACCC AGCAGCGGCT CAGCCTGTAC 
CGACGCTCAG AGCGCCAGGA GCTCTCAGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGA 
CTGCCCACCA CTGGCTATGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AACGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAACCCA ACGACATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA TGTGGCCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAGCTCT CATCCTGTAA TCCTGAGACC AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497883.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497946.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu73352
Clone ID Related Accession (Same CDS sequence)	XM_006497883.4 , XM_006497883.3
Accession Version	XM_006497883.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497883.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGT CCTTCGTGAA GGACTACATG ATCACCATCA CCAGGCTGCT CTTGGGCCTT 
GATACTACAC CAGGCTCCGG CTACCTCTGT GCAATGAAGG TAACCGAGGA CGACCTGTGG 
ATCCGCACTT ACGGCCGCCT CTTCCAGAAA CTCTGCTCCT CCAGCGCCGA GATCCCCATC 
GGCATCTACA GGACCGAGTG CCATGTCTTC TCGGAGCCCC ATGACGTCAG AGCCCAGGGG 
GGCCCAGACA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGACTGC 
GAGGATACTC GGGAGGCCAA GGGACCCTGG GGCACACGAG CTGCATCTGG CAGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCAGTGGAGC ACCCACTACT ACGTCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGTCGC AAGAGCACCA AGCAGGCAGG GAAGGCACCT 
GTGGCCACAG ACTGGATCAC CCAGCAGCGG CTCAGCCTGT ACCGACGCTC AGAGCGCCAG 
GAGCTCTCAG AGCTGGTCAA GAACCGAATG AAGCACCTGG GACTGCCCAC CACTGGCTAT 
GATGAGATGA ACGATCATCA CCAGAACACC CTTTCCTATG TACTCATCAA CCCCCCGCCA 
GACACAAGAC TGGAACCCAA CGACATTGTG TACCTCATCC GTTCCGACCC CCTGGCCCAT 
GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT 
CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497946.2
CDS	202..3891
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497883.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497883.4

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGT CCTTCGTGAA GGACTACATG ATCACCATCA CCAGGCTGCT CTTGGGCCTT 
GATACTACAC CAGGCTCCGG CTACCTCTGT GCAATGAAGG TAACCGAGGA CGACCTGTGG 
ATCCGCACTT ACGGCCGCCT CTTCCAGAAA CTCTGCTCCT CCAGCGCCGA GATCCCCATC 
GGCATCTACA GGACCGAGTG CCATGTCTTC TCGGAGCCCC ATGACGTCAG AGCCCAGGGG 
GGCCCAGACA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGACTGC 
GAGGATACTC GGGAGGCCAA GGGACCCTGG GGCACACGAG CTGCATCTGG CAGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCAGTGGAGC ACCCACTACT ACGTCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGTCGC AAGAGCACCA AGCAGGCAGG GAAGGCACCT 
GTGGCCACAG ACTGGATCAC CCAGCAGCGG CTCAGCCTGT ACCGACGCTC AGAGCGCCAG 
GAGCTCTCAG AGCTGGTCAA GAACCGAATG AAGCACCTGG GACTGCCCAC CACTGGCTAT 
GATGAGATGA ACGATCATCA CCAGAACACC CTTTCCTATG TACTCATCAA CCCCCCGCCA 
GACACAAGAC TGGAACCCAA CGACATTGTG TACCTCATCC GTTCCGACCC CCTGGCCCAT 
GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT 
CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497884.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497947.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X10, mRNA.

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CloneID	OMu73353
Clone ID Related Accession (Same CDS sequence)	XM_006497884.4 , XM_006497884.3
Accession Version	XM_006497884.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497884.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAACC TGGACAGCTT ACTGCAGTGT GGCATCATCT ATGCTGACAA CCTGGTGGTG 
GTGGACAAGG AGAGCACTAT GAGCGCTGAA GAGGACTACA TGGCAGATGC CAAGACCATC 
GTCAATGTGC AAACCATGTT CCGGCTTTTC CCCAGTCTCA GCATCACCAC GGAGCTCACA 
CACCCTTCCA ACATGCGGTT CATGCAGTTC CGTGCCAAGG ACAGCTACTC TCTGGCTCTT 
TCCAAACTTG AAAAGCAAGA ACGGGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG 
CCATTTGCTG CTGGTCGAGT ATTTAGTATC AGCATGTTGG ATACACTGCT CTACCAGTCC 
TTCGTGAAGG ACTACATGAT CACCATCACC AGGCTGCTCT TGGGCCTTGA TACTACACCA 
GGCTCCGGCT ACCTCTGTGC AATGAAGGTA ACCGAGGACG ACCTGTGGAT CCGCACTTAC 
GGCCGCCTCT TCCAGAAACT CTGCTCCTCC AGCGCCGAGA TCCCCATCGG CATCTACAGG 
ACCGAGTGCC ATGTCTTCTC GGAGCCCCAT GACGTCAGAG CCCAGGGGGG CCCAGACATA 
GCCCTCCTGC CTGCCCAGTC TCAGATCTCG GTGAACATGG AGGACTGCGA GGATACTCGG 
GAGGCCAAGG GACCCTGGGG CACACGAGCT GCATCTGGCA GTGGCAGCAC CCATGGCCGT 
CACGGGGGCA GTGCTGACCC AGTGGAGCAC CCACTACTAC GTCGCAAGAG CCTGCAGTGG 
GCCCGCAAGC TGAGTCGCAA GAGCACCAAG CAGGCAGGGA AGGCACCTGT GGCCACAGAC 
TGGATCACCC AGCAGCGGCT CAGCCTGTAC CGACGCTCAG AGCGCCAGGA GCTCTCAGAG 
CTGGTCAAGA ACCGAATGAA GCACCTGGGA CTGCCCACCA CTGGCTATGA GGACGTAGCA 
AATTTAACAG CCAGTGATGT GATGAATCGG GTAAACCTGG GATATTTGCA AGATGAGATG 
AACGATCATC ACCAGAACAC CCTTTCCTAT GTACTCATCA ACCCCCCGCC AGACACAAGA 
CTGGAACCCA ACGACATTGT GTACCTCATC CGTTCCGACC CCCTGGCCCA TGTGGCCAGC 
AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC AACAAGCTCT CATCCTGTAA TCCTGAGACC 
AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497947.2
CDS	201..3881
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497884.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497884.4

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAACC TGGACAGCTT ACTGCAGTGT GGCATCATCT ATGCTGACAA CCTGGTGGTG 
GTGGACAAGG AGAGCACTAT GAGCGCTGAA GAGGACTACA TGGCAGATGC CAAGACCATC 
GTCAATGTGC AAACCATGTT CCGGCTTTTC CCCAGTCTCA GCATCACCAC GGAGCTCACA 
CACCCTTCCA ACATGCGGTT CATGCAGTTC CGTGCCAAGG ACAGCTACTC TCTGGCTCTT 
TCCAAACTTG AAAAGCAAGA ACGGGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG 
CCATTTGCTG CTGGTCGAGT ATTTAGTATC AGCATGTTGG ATACACTGCT CTACCAGTCC 
TTCGTGAAGG ACTACATGAT CACCATCACC AGGCTGCTCT TGGGCCTTGA TACTACACCA 
GGCTCCGGCT ACCTCTGTGC AATGAAGGTA ACCGAGGACG ACCTGTGGAT CCGCACTTAC 
GGCCGCCTCT TCCAGAAACT CTGCTCCTCC AGCGCCGAGA TCCCCATCGG CATCTACAGG 
ACCGAGTGCC ATGTCTTCTC GGAGCCCCAT GACGTCAGAG CCCAGGGGGG CCCAGACATA 
GCCCTCCTGC CTGCCCAGTC TCAGATCTCG GTGAACATGG AGGACTGCGA GGATACTCGG 
GAGGCCAAGG GACCCTGGGG CACACGAGCT GCATCTGGCA GTGGCAGCAC CCATGGCCGT 
CACGGGGGCA GTGCTGACCC AGTGGAGCAC CCACTACTAC GTCGCAAGAG CCTGCAGTGG 
GCCCGCAAGC TGAGTCGCAA GAGCACCAAG CAGGCAGGGA AGGCACCTGT GGCCACAGAC 
TGGATCACCC AGCAGCGGCT CAGCCTGTAC CGACGCTCAG AGCGCCAGGA GCTCTCAGAG 
CTGGTCAAGA ACCGAATGAA GCACCTGGGA CTGCCCACCA CTGGCTATGA GGACGTAGCA 
AATTTAACAG CCAGTGATGT GATGAATCGG GTAAACCTGG GATATTTGCA AGATGAGATG 
AACGATCATC ACCAGAACAC CCTTTCCTAT GTACTCATCA ACCCCCCGCC AGACACAAGA 
CTGGAACCCA ACGACATTGT GTACCTCATC CGTTCCGACC CCCTGGCCCA TGTGGCCAGC 
AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC AACAAGCTCT CATCCTGTAA TCCTGAGACC 
AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497886.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497949.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu73354
Clone ID Related Accession (Same CDS sequence)	XM_006497886.3 , XM_006497886.4
Accession Version	XM_006497886.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497886.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGAA TGACTTCCAC CGTGCCATCC TACGCACACA GTCAGCCATG 
TTCAACCAGG TGCTCATCCT GTTCTGCACC CTGCTGTGCC TGGTCTTCAC AGGGACCTGT 
GGGATCCAGC ACCTGGAGCG AGCAGGTGGC AACTTGAACC TGCTGACCTC CTTCTACTTC 
TGCATCGTGA CTTTCTCAAC TGTGGGCTTC GGTGATGTGA CGCCCAAGAT CTGGCCATCC 
CAGCTCCTGG TGGTCATCCT GATCTGTGTC ACCCTTGTGG TGCTCCCACT GCAATTTGAA 
GAGCTTGTCT ACCTCTGGAT GGAGCGTCAG AAGTCAGGGG GCAACTATAG CCGCCACCGA 
GCACGGACGG AGAAGCACGT AGTCCTGTGT GTGAGCTCCC TCAAGATTGA TCTCCTCATG 
GACTTCCTGA ATGAGTTCTA TGCCCATCCC CGGCTCCAGC AGGACTACTA CGTGGTCATC 
CTGTGTCCCT CTGAAATGGA CGTCCAGGTG CGCAGGGTGC TGCAGATTCC CCTGTGGTCC 
CAGCGGGTCA TCTACCTCCA GGGCTCTGCC CTCAAGGACC AGGATCTCAT GCGAGCCAAG 
ATGGACAACG GAGAGGCCTG CTTTATCCTC AGCAGCAGGA ATGAGGTGGA CCGCACAGCT 
GCGGATCACC AGACCATCCT TCGAGCCTGG GCTGTGAAGG ACTTTGCCCC CAACTGTCCC 
CTCTATGTCC AGATCCTCAA GCCCGAAAAC AAGTTTCACG TCAAATTTGC TGACCACGTG 
GTATGCGAGG AAGAGTGCAA GTACGCCATG CTGGCCCTGA ACTGCATCTG CCCGGCCACC 
TCCACCCTCA TCACCCTGCT GGTGCACACG TCCCGTGGCC AGGAAGGACA GGAGTCTCCC 
GAGCAGTGGC AGCGCACGTA CGGGAGGTGC TCGGGCAACG AGGTGTACCA CATTCGCATG 
GGTGACAGTA AATTCTTCCG GGAGTATGAG GGCAAGAGCT TCACCTACGC AGCCTTCCAC 
GCGCACAAGA AATATGGGGT GTGCCTCATC GGGCTGAAGC GTGAGGAGAA CAAGAGTATC 
CTGCTGAACC CAGGACCACG GCACATCCTG GCTGCCTCCG ACACCTGCTT CTATATCAAT 
ATTACCAAGG AGGAGAACTC AGCTTTCATC TTCAAACAGG AGGAGAAGCA GAAGAGACGG 
GGCCTTGCAG GGCAGGCACT ATATGAAGGG CCCTCCCGGC TCCCAGTGCA TAGCATCATC 
GCCTCTATGG GGACAGTGGC CATGGACCTG CAGAACACAG ATTGCCGGCC CTCCCAGGGT 
GGCAGTGGCG GGGACGGCAC AAAGCTGACT CTGCCCACCG AGAACGGCTC TGGTAGTCGA 
CGCCCCAGCA TCGCACCCGT TCTGGAGTTG GCAGACAGCT CAGCCCTGTT GCCCTGCGAC 
CTGCTGAGTG ACCAGTCAGA GGATGAGGTG ACACCCTCGG ACGACGAGGG GCTCTCTGTG 
GTTGAGTACG TGAAGGGCTA TCCCCCCAAC TCACCCTACA TTGGCAGCTC CCCGACTTTA 
TGCCACCTCC TGCCTGTGAA AGCCCCCTTC TGCTGCCTGC GGTTGGACAA GGGCTGCAAA 
CACAACAGCT ACGAGGATGC CAAGGCCTAT GGGTTCAAGA ACAAGCTGAT TATTGTCTCT 
GCTGAGACGG CAGGCAACGG GCTCTACAAC TTCATCGTGC CTCTGCGCGC CTACTACCGG 
TCCCGCCGGG AGCTCAACCC TATCGTGTTG CTGCTTGACA ACAAGCCTGA CCACCACTTC 
CTGGAGGCCA TCTGTTGCTT CCCCATGGTC TACTACATGG AGGGATCCGT GGACAACCTG 
GACAGCTTAC TGCAGTGTGG CATCATCTAT GCTGACAACC TGGTGGTGGT GGACAAGGAG 
AGCACTATGA GCGCTGAAGA GGACTACATG GCAGATGCCA AGACCATCGT CAATGTGCAA 
ACCATGTTCC GGCTTTTCCC CAGTCTCAGC ATCACCACGG AGCTCACACA CCCTTCCAAC 
ATGCGGTTCA TGCAGTTCCG TGCCAAGGAC AGCTACTCTC TGGCTCTTTC CAAACTTGAA 
AAGCAAGAAC GGGAGAACGG CTCCAACCTG GCCTTCATGT TCCGCCTGCC ATTTGCTGCT 
GGTCGAGTAT TTAGTATCAG CATGTTGGAT ACACTGCTCT ACCAGTCCTT CGTGAAGGAC 
TACATGATCA CCATCACCAG GCTGCTCTTG GGCCTTGATA CTACACCAGG CTCCGGCTAC 
CTCTGTGCAA TGAAGGTAAC CGAGGACGAC CTGTGGATCC GCACTTACGG CCGCCTCTTC 
CAGAAACTCT GCTCCTCCAG CGCCGAGATC CCCATCGGCA TCTACAGGAC CGAGTGCCAT 
GTCTTCTCGG AGCCCCATGA CGTCAGAGCC CAGGGGGGCC CAGACATAGC CCTCCTGCCT 
GCCCAGTCTC AGATCTCGGT GAACATGGAG GACTGCGAGG ATACTCGGGA GGCCAAGGGA 
CCCTGGGGCA CACGAGCTGC ATCTGGCAGT GGCAGCACCC ATGGCCGTCA CGGGGGCAGT 
GCTGACCCAG TGGAGCACCC ACTACTACGT CGCAAGAGCC TGCAGTGGGC CCGCAAGCTG 
AGTCGCAAGA GCACCAAGCA GGCAGGGAAG GCACCTGTGG CCACAGACTG GATCACCCAG 
CAGCGGCTCA GCCTGTACCG ACGCTCAGAG CGCCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGAATGAAGC ACCTGGGACT GCCCACCACT GGCTATGAGG ACGTAGCAAA TTTAACAGCC 
AGTGATGTGA TGAATCGGGT AAACCTGGGA TATTTGCAAG ATGAGATGAA CGATCATCAC 
CAGAACACCC TTTCCTATGT ACTCATCAAC CCCCCGCCAG ACACAAGACT GGAACCCAAC 
GACATTGTGT ACCTCATCCG TTCCGACCCC CTGGCCCATG TGGCCAGCAG CTCCCAGAGT 
CGGAAAAGCA GCTGCAGCAA CAAGCTCTCA TCCTGTAATC CTGAGACCAG GGATGAGACC 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497949.2
CDS	59..3727
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITNDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497886.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497886.4

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGAA TGACTTCCAC CGTGCCATCC TACGCACACA GTCAGCCATG 
TTCAACCAGG TGCTCATCCT GTTCTGCACC CTGCTGTGCC TGGTCTTCAC AGGGACCTGT 
GGGATCCAGC ACCTGGAGCG AGCAGGTGGC AACTTGAACC TGCTGACCTC CTTCTACTTC 
TGCATCGTGA CTTTCTCAAC TGTGGGCTTC GGTGATGTGA CGCCCAAGAT CTGGCCATCC 
CAGCTCCTGG TGGTCATCCT GATCTGTGTC ACCCTTGTGG TGCTCCCACT GCAATTTGAA 
GAGCTTGTCT ACCTCTGGAT GGAGCGTCAG AAGTCAGGGG GCAACTATAG CCGCCACCGA 
GCACGGACGG AGAAGCACGT AGTCCTGTGT GTGAGCTCCC TCAAGATTGA TCTCCTCATG 
GACTTCCTGA ATGAGTTCTA TGCCCATCCC CGGCTCCAGC AGGACTACTA CGTGGTCATC 
CTGTGTCCCT CTGAAATGGA CGTCCAGGTG CGCAGGGTGC TGCAGATTCC CCTGTGGTCC 
CAGCGGGTCA TCTACCTCCA GGGCTCTGCC CTCAAGGACC AGGATCTCAT GCGAGCCAAG 
ATGGACAACG GAGAGGCCTG CTTTATCCTC AGCAGCAGGA ATGAGGTGGA CCGCACAGCT 
GCGGATCACC AGACCATCCT TCGAGCCTGG GCTGTGAAGG ACTTTGCCCC CAACTGTCCC 
CTCTATGTCC AGATCCTCAA GCCCGAAAAC AAGTTTCACG TCAAATTTGC TGACCACGTG 
GTATGCGAGG AAGAGTGCAA GTACGCCATG CTGGCCCTGA ACTGCATCTG CCCGGCCACC 
TCCACCCTCA TCACCCTGCT GGTGCACACG TCCCGTGGCC AGGAAGGACA GGAGTCTCCC 
GAGCAGTGGC AGCGCACGTA CGGGAGGTGC TCGGGCAACG AGGTGTACCA CATTCGCATG 
GGTGACAGTA AATTCTTCCG GGAGTATGAG GGCAAGAGCT TCACCTACGC AGCCTTCCAC 
GCGCACAAGA AATATGGGGT GTGCCTCATC GGGCTGAAGC GTGAGGAGAA CAAGAGTATC 
CTGCTGAACC CAGGACCACG GCACATCCTG GCTGCCTCCG ACACCTGCTT CTATATCAAT 
ATTACCAAGG AGGAGAACTC AGCTTTCATC TTCAAACAGG AGGAGAAGCA GAAGAGACGG 
GGCCTTGCAG GGCAGGCACT ATATGAAGGG CCCTCCCGGC TCCCAGTGCA TAGCATCATC 
GCCTCTATGG GGACAGTGGC CATGGACCTG CAGAACACAG ATTGCCGGCC CTCCCAGGGT 
GGCAGTGGCG GGGACGGCAC AAAGCTGACT CTGCCCACCG AGAACGGCTC TGGTAGTCGA 
CGCCCCAGCA TCGCACCCGT TCTGGAGTTG GCAGACAGCT CAGCCCTGTT GCCCTGCGAC 
CTGCTGAGTG ACCAGTCAGA GGATGAGGTG ACACCCTCGG ACGACGAGGG GCTCTCTGTG 
GTTGAGTACG TGAAGGGCTA TCCCCCCAAC TCACCCTACA TTGGCAGCTC CCCGACTTTA 
TGCCACCTCC TGCCTGTGAA AGCCCCCTTC TGCTGCCTGC GGTTGGACAA GGGCTGCAAA 
CACAACAGCT ACGAGGATGC CAAGGCCTAT GGGTTCAAGA ACAAGCTGAT TATTGTCTCT 
GCTGAGACGG CAGGCAACGG GCTCTACAAC TTCATCGTGC CTCTGCGCGC CTACTACCGG 
TCCCGCCGGG AGCTCAACCC TATCGTGTTG CTGCTTGACA ACAAGCCTGA CCACCACTTC 
CTGGAGGCCA TCTGTTGCTT CCCCATGGTC TACTACATGG AGGGATCCGT GGACAACCTG 
GACAGCTTAC TGCAGTGTGG CATCATCTAT GCTGACAACC TGGTGGTGGT GGACAAGGAG 
AGCACTATGA GCGCTGAAGA GGACTACATG GCAGATGCCA AGACCATCGT CAATGTGCAA 
ACCATGTTCC GGCTTTTCCC CAGTCTCAGC ATCACCACGG AGCTCACACA CCCTTCCAAC 
ATGCGGTTCA TGCAGTTCCG TGCCAAGGAC AGCTACTCTC TGGCTCTTTC CAAACTTGAA 
AAGCAAGAAC GGGAGAACGG CTCCAACCTG GCCTTCATGT TCCGCCTGCC ATTTGCTGCT 
GGTCGAGTAT TTAGTATCAG CATGTTGGAT ACACTGCTCT ACCAGTCCTT CGTGAAGGAC 
TACATGATCA CCATCACCAG GCTGCTCTTG GGCCTTGATA CTACACCAGG CTCCGGCTAC 
CTCTGTGCAA TGAAGGTAAC CGAGGACGAC CTGTGGATCC GCACTTACGG CCGCCTCTTC 
CAGAAACTCT GCTCCTCCAG CGCCGAGATC CCCATCGGCA TCTACAGGAC CGAGTGCCAT 
GTCTTCTCGG AGCCCCATGA CGTCAGAGCC CAGGGGGGCC CAGACATAGC CCTCCTGCCT 
GCCCAGTCTC AGATCTCGGT GAACATGGAG GACTGCGAGG ATACTCGGGA GGCCAAGGGA 
CCCTGGGGCA CACGAGCTGC ATCTGGCAGT GGCAGCACCC ATGGCCGTCA CGGGGGCAGT 
GCTGACCCAG TGGAGCACCC ACTACTACGT CGCAAGAGCC TGCAGTGGGC CCGCAAGCTG 
AGTCGCAAGA GCACCAAGCA GGCAGGGAAG GCACCTGTGG CCACAGACTG GATCACCCAG 
CAGCGGCTCA GCCTGTACCG ACGCTCAGAG CGCCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGAATGAAGC ACCTGGGACT GCCCACCACT GGCTATGAGG ACGTAGCAAA TTTAACAGCC 
AGTGATGTGA TGAATCGGGT AAACCTGGGA TATTTGCAAG ATGAGATGAA CGATCATCAC 
CAGAACACCC TTTCCTATGT ACTCATCAAC CCCCCGCCAG ACACAAGACT GGAACCCAAC 
GACATTGTGT ACCTCATCCG TTCCGACCCC CTGGCCCATG TGGCCAGCAG CTCCCAGAGT 
CGGAAAAGCA GCTGCAGCAA CAAGCTCTCA TCCTGTAATC CTGAGACCAG GGATGAGACC 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497889.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497952.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OMu73355
Clone ID Related Accession (Same CDS sequence)	XM_006497889.4 , XM_006497889.3
Accession Version	XM_006497889.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497889.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGGGAC AGTGGCCATG GACCTGCAGA ACACAGATTG CCGGCCCTCC 
CAGGGTGGCA GTGGCGGGGA CGGCACAAAG CTGACTCTGC CCACCGAGAA CGGCTCTGGT 
AGTCGACGCC CCAGCATCGC ACCCGTTCTG GAGTTGGCAG ACAGCTCAGC CCTGTTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAT GAGGTGACAC CCTCGGACGA CGAGGGGCTC 
TCTGTGGTTG AGTACGTGAA GGGCTATCCC CCCAACTCAC CCTACATTGG CAGCTCCCCG 
ACTTTATGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGTT GGACAAGGGC 
TGCAAACACA ACAGCTACGA GGATGCCAAG GCCTATGGGT TCAAGAACAA GCTGATTATT 
GTCTCTGCTG AGACGGCAGG CAACGGGCTC TACAACTTCA TCGTGCCTCT GCGCGCCTAC 
TACCGGTCCC GCCGGGAGCT CAACCCTATC GTGTTGCTGC TTGACAACAA GCCTGACCAC 
CACTTCCTGG AGGCCATCTG TTGCTTCCCC ATGGTCTACT ACATGGAGGG ATCCGTGGAC 
AACCTGGACA GCTTACTGCA GTGTGGCATC ATCTATGCTG ACAACCTGGT GGTGGTGGAC 
AAGGAGAGCA CTATGAGCGC TGAAGAGGAC TACATGGCAG ATGCCAAGAC CATCGTCAAT 
GTGCAAACCA TGTTCCGGCT TTTCCCCAGT CTCAGCATCA CCACGGAGCT CACACACCCT 
TCCAACATGC GGTTCATGCA GTTCCGTGCC AAGGACAGCT ACTCTCTGGC TCTTTCCAAA 
CTTGAAAAGC AAGAACGGGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCATTT 
GCTGCTGGTC GAGTATTTAG TATCAGCATG TTGGATACAC TGCTCTACCA GTCCTTCGTG 
AAGGACTACA TGATCACCAT CACCAGGCTG CTCTTGGGCC TTGATACTAC ACCAGGCTCC 
GGCTACCTCT GTGCAATGAA GGTAACCGAG GACGACCTGT GGATCCGCAC TTACGGCCGC 
CTCTTCCAGA AACTCTGCTC CTCCAGCGCC GAGATCCCCA TCGGCATCTA CAGGACCGAG 
TGCCATGTCT TCTCGGAGCC CCATGACGTC AGAGCCCAGG GGGGCCCAGA CATAGCCCTC 
CTGCCTGCCC AGTCTCAGAT CTCGGTGAAC ATGGAGGACT GCGAGGATAC TCGGGAGGCC 
AAGGGACCCT GGGGCACACG AGCTGCATCT GGCAGTGGCA GCACCCATGG CCGTCACGGG 
GGCAGTGCTG ACCCAGTGGA GCACCCACTA CTACGTCGCA AGAGCCTGCA GTGGGCCCGC 
AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA GGGAAGGCAC CTGTGGCCAC AGACTGGATC 
ACCCAGCAGC GGCTCAGCCT GTACCGACGC TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGAA TGAAGCACCT GGGACTGCCC ACCACTGGCT ATGAGGACGT AGCAAATTTA 
ACAGCCAGTG ATGTGATGAA TCGGGTAAAC CTGGGATATT TGCAAGATGA GATGAACGAT 
CATCACCAGA ACACCCTTTC CTATGTACTC ATCAACCCCC CGCCAGACAC AAGACTGGAA 
CCCAACGACA TTGTGTACCT CATCCGTTCC GACCCCCTGG CCCATGTGGC CAGCAGCTCC 
CAGAGTCGGA AAAGCAGCTG CAGCAACAAG CTCTCATCCT GTAATCCTGA GACCAGGGAT 
GAGACCCAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497952.2
CDS	200..3454
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497889.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497889.4

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGGGAC AGTGGCCATG GACCTGCAGA ACACAGATTG CCGGCCCTCC 
CAGGGTGGCA GTGGCGGGGA CGGCACAAAG CTGACTCTGC CCACCGAGAA CGGCTCTGGT 
AGTCGACGCC CCAGCATCGC ACCCGTTCTG GAGTTGGCAG ACAGCTCAGC CCTGTTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAT GAGGTGACAC CCTCGGACGA CGAGGGGCTC 
TCTGTGGTTG AGTACGTGAA GGGCTATCCC CCCAACTCAC CCTACATTGG CAGCTCCCCG 
ACTTTATGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGTT GGACAAGGGC 
TGCAAACACA ACAGCTACGA GGATGCCAAG GCCTATGGGT TCAAGAACAA GCTGATTATT 
GTCTCTGCTG AGACGGCAGG CAACGGGCTC TACAACTTCA TCGTGCCTCT GCGCGCCTAC 
TACCGGTCCC GCCGGGAGCT CAACCCTATC GTGTTGCTGC TTGACAACAA GCCTGACCAC 
CACTTCCTGG AGGCCATCTG TTGCTTCCCC ATGGTCTACT ACATGGAGGG ATCCGTGGAC 
AACCTGGACA GCTTACTGCA GTGTGGCATC ATCTATGCTG ACAACCTGGT GGTGGTGGAC 
AAGGAGAGCA CTATGAGCGC TGAAGAGGAC TACATGGCAG ATGCCAAGAC CATCGTCAAT 
GTGCAAACCA TGTTCCGGCT TTTCCCCAGT CTCAGCATCA CCACGGAGCT CACACACCCT 
TCCAACATGC GGTTCATGCA GTTCCGTGCC AAGGACAGCT ACTCTCTGGC TCTTTCCAAA 
CTTGAAAAGC AAGAACGGGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCATTT 
GCTGCTGGTC GAGTATTTAG TATCAGCATG TTGGATACAC TGCTCTACCA GTCCTTCGTG 
AAGGACTACA TGATCACCAT CACCAGGCTG CTCTTGGGCC TTGATACTAC ACCAGGCTCC 
GGCTACCTCT GTGCAATGAA GGTAACCGAG GACGACCTGT GGATCCGCAC TTACGGCCGC 
CTCTTCCAGA AACTCTGCTC CTCCAGCGCC GAGATCCCCA TCGGCATCTA CAGGACCGAG 
TGCCATGTCT TCTCGGAGCC CCATGACGTC AGAGCCCAGG GGGGCCCAGA CATAGCCCTC 
CTGCCTGCCC AGTCTCAGAT CTCGGTGAAC ATGGAGGACT GCGAGGATAC TCGGGAGGCC 
AAGGGACCCT GGGGCACACG AGCTGCATCT GGCAGTGGCA GCACCCATGG CCGTCACGGG 
GGCAGTGCTG ACCCAGTGGA GCACCCACTA CTACGTCGCA AGAGCCTGCA GTGGGCCCGC 
AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA GGGAAGGCAC CTGTGGCCAC AGACTGGATC 
ACCCAGCAGC GGCTCAGCCT GTACCGACGC TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGAA TGAAGCACCT GGGACTGCCC ACCACTGGCT ATGAGGACGT AGCAAATTTA 
ACAGCCAGTG ATGTGATGAA TCGGGTAAAC CTGGGATATT TGCAAGATGA GATGAACGAT 
CATCACCAGA ACACCCTTTC CTATGTACTC ATCAACCCCC CGCCAGACAC AAGACTGGAA 
CCCAACGACA TTGTGTACCT CATCCGTTCC GACCCCCTGG CCCATGTGGC CAGCAGCTCC 
CAGAGTCGGA AAAGCAGCTG CAGCAACAAG CTCTCATCCT GTAATCCTGA GACCAGGGAT 
GAGACCCAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_017317195.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172684.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X9, mRNA.

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CloneID	OMu80185
Clone ID Related Accession (Same CDS sequence)	XM_017317195.1 , XM_017317195.2
Accession Version	XM_017317195.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAACCTGG ACAGCTTACT GCAGTGTGGC ATCATCTATG CTGACAACCT GGTGGTGGTG 
GACAAGGAGA GCACTATGAG CGCTGAAGAG GACTACATGG CAGATGCCAA GACCATCGTC 
AATGTGCAAA CCATGTTCCG GCTTTTCCCC AGTCTCAGCA TCACCACGGA GCTCACACAC 
CCTTCCAACA TGCGGTTCAT GCAGTTCCGT GCCAAGGACA GCTACTCTCT GGCTCTTTCC 
AAACTTGAAA AGCAAGAACG GGAGAACGGC TCCAACCTGG CCTTCATGTT CCGCCTGCCA 
TTTGCTGCTG GTCGAGTATT TAGTATCAGC ATGTTGGATA CACTGCTCTA CCAGTCCTTC 
GTGAAGGACT ACATGATCAC CATCACCAGG CTGCTCTTGG GCCTTGATAC TACACCAGGC 
TCCGGCTACC TCTGTGCAAT GAAGGTAACC GAGGACGACC TGTGGATCCG CACTTACGGC 
CGCCTCTTCC AGAAACTCTG CTCCTCCAGC GCCGAGATCC CCATCGGCAT CTACAGGACC 
GAGTGCCATG TCTTCTCGGA GCCCCATGAC GTCAGAGCCC AGGGGGGCCC AGACATAGCC 
CTCCTGCCTG CCCAGTCTCA GATCTCGGTG AACATGGAGG ACTGCGAGGA TACTCGGGAG 
GCCAAGGGAC CCTGGGGCAC ACGAGCTGCA TCTGGCAGTG GCAGCACCCA TGGCCGTCAC 
GGGGGCAGTG CTGACCCAGT GGAGCACCCA CTACTACGTC GCAAGAGCCT GCAGTGGGCC 
CGCAAGCTGA GTCGCAAGAG CACCAAGCAG GCAGGGAAGG CACCTGTGGC CACAGACTGG 
ATCACCCAGC AGCGGCTCAG CCTGTACCGA CGCTCAGAGC GCCAGGAGCT CTCAGAGCTG 
GTCAAGAACC GAATGAAGCA CCTGGGACTG CCCACCACTG GCTATGAGGA CGTAGCAAAT 
TTAACAGCCA GTGATGTGAT GAATCGGGTA AACCTGGGAT ATTTGCAAGA TGAGATGAAC 
GATCATCACC AGAACACCCT TTCCTATGTA CTCATCAACC CCCCGCCAGA CACAAGACTG 
GAACCCAACG ACATTGTGTA CCTCATCCGT TCCGACCCCC TGGCCCATGT GGCCAGCAGC 
TCCCAGAGTC GGAAAAGCAG CTGCAGCAAC AAGCTCTCAT CCTGTAATCC TGAGACCAGG 
GATGAGACCC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172684.1
CDS	202..3879
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_017317195.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317195.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAACCTGG ACAGCTTACT GCAGTGTGGC ATCATCTATG CTGACAACCT GGTGGTGGTG 
GACAAGGAGA GCACTATGAG CGCTGAAGAG GACTACATGG CAGATGCCAA GACCATCGTC 
AATGTGCAAA CCATGTTCCG GCTTTTCCCC AGTCTCAGCA TCACCACGGA GCTCACACAC 
CCTTCCAACA TGCGGTTCAT GCAGTTCCGT GCCAAGGACA GCTACTCTCT GGCTCTTTCC 
AAACTTGAAA AGCAAGAACG GGAGAACGGC TCCAACCTGG CCTTCATGTT CCGCCTGCCA 
TTTGCTGCTG GTCGAGTATT TAGTATCAGC ATGTTGGATA CACTGCTCTA CCAGTCCTTC 
GTGAAGGACT ACATGATCAC CATCACCAGG CTGCTCTTGG GCCTTGATAC TACACCAGGC 
TCCGGCTACC TCTGTGCAAT GAAGGTAACC GAGGACGACC TGTGGATCCG CACTTACGGC 
CGCCTCTTCC AGAAACTCTG CTCCTCCAGC GCCGAGATCC CCATCGGCAT CTACAGGACC 
GAGTGCCATG TCTTCTCGGA GCCCCATGAC GTCAGAGCCC AGGGGGGCCC AGACATAGCC 
CTCCTGCCTG CCCAGTCTCA GATCTCGGTG AACATGGAGG ACTGCGAGGA TACTCGGGAG 
GCCAAGGGAC CCTGGGGCAC ACGAGCTGCA TCTGGCAGTG GCAGCACCCA TGGCCGTCAC 
GGGGGCAGTG CTGACCCAGT GGAGCACCCA CTACTACGTC GCAAGAGCCT GCAGTGGGCC 
CGCAAGCTGA GTCGCAAGAG CACCAAGCAG GCAGGGAAGG CACCTGTGGC CACAGACTGG 
ATCACCCAGC AGCGGCTCAG CCTGTACCGA CGCTCAGAGC GCCAGGAGCT CTCAGAGCTG 
GTCAAGAACC GAATGAAGCA CCTGGGACTG CCCACCACTG GCTATGAGGA CGTAGCAAAT 
TTAACAGCCA GTGATGTGAT GAATCGGGTA AACCTGGGAT ATTTGCAAGA TGAGATGAAC 
GATCATCACC AGAACACCCT TTCCTATGTA CTCATCAACC CCCCGCCAGA CACAAGACTG 
GAACCCAACG ACATTGTGTA CCTCATCCGT TCCGACCCCC TGGCCCATGT GGCCAGCAGC 
TCCCAGAGTC GGAAAAGCAG CTGCAGCAAC AAGCTCTCAT CCTGTAATCC TGAGACCAGG 
GATGAGACCC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_017317195.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172684.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X11, mRNA.

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CloneID	OMu80185
Clone ID Related Accession (Same CDS sequence)	XM_017317195.1 , XM_017317195.2
Accession Version	XM_017317195.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAACCTGG ACAGCTTACT GCAGTGTGGC ATCATCTATG CTGACAACCT GGTGGTGGTG 
GACAAGGAGA GCACTATGAG CGCTGAAGAG GACTACATGG CAGATGCCAA GACCATCGTC 
AATGTGCAAA CCATGTTCCG GCTTTTCCCC AGTCTCAGCA TCACCACGGA GCTCACACAC 
CCTTCCAACA TGCGGTTCAT GCAGTTCCGT GCCAAGGACA GCTACTCTCT GGCTCTTTCC 
AAACTTGAAA AGCAAGAACG GGAGAACGGC TCCAACCTGG CCTTCATGTT CCGCCTGCCA 
TTTGCTGCTG GTCGAGTATT TAGTATCAGC ATGTTGGATA CACTGCTCTA CCAGTCCTTC 
GTGAAGGACT ACATGATCAC CATCACCAGG CTGCTCTTGG GCCTTGATAC TACACCAGGC 
TCCGGCTACC TCTGTGCAAT GAAGGTAACC GAGGACGACC TGTGGATCCG CACTTACGGC 
CGCCTCTTCC AGAAACTCTG CTCCTCCAGC GCCGAGATCC CCATCGGCAT CTACAGGACC 
GAGTGCCATG TCTTCTCGGA GCCCCATGAC GTCAGAGCCC AGGGGGGCCC AGACATAGCC 
CTCCTGCCTG CCCAGTCTCA GATCTCGGTG AACATGGAGG ACTGCGAGGA TACTCGGGAG 
GCCAAGGGAC CCTGGGGCAC ACGAGCTGCA TCTGGCAGTG GCAGCACCCA TGGCCGTCAC 
GGGGGCAGTG CTGACCCAGT GGAGCACCCA CTACTACGTC GCAAGAGCCT GCAGTGGGCC 
CGCAAGCTGA GTCGCAAGAG CACCAAGCAG GCAGGGAAGG CACCTGTGGC CACAGACTGG 
ATCACCCAGC AGCGGCTCAG CCTGTACCGA CGCTCAGAGC GCCAGGAGCT CTCAGAGCTG 
GTCAAGAACC GAATGAAGCA CCTGGGACTG CCCACCACTG GCTATGAGGA CGTAGCAAAT 
TTAACAGCCA GTGATGTGAT GAATCGGGTA AACCTGGGAT ATTTGCAAGA TGAGATGAAC 
GATCATCACC AGAACACCCT TTCCTATGTA CTCATCAACC CCCCGCCAGA CACAAGACTG 
GAACCCAACG ACATTGTGTA CCTCATCCGT TCCGACCCCC TGGCCCATGT GGCCAGCAGC 
TCCCAGAGTC GGAAAAGCAG CTGCAGCAAC AAGCTCTCAT CCTGTAATCC TGAGACCAGG 
GATGAGACCC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172684.1
CDS	200..3877
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_017317195.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317195.2

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAACCTGG ACAGCTTACT GCAGTGTGGC ATCATCTATG CTGACAACCT GGTGGTGGTG 
GACAAGGAGA GCACTATGAG CGCTGAAGAG GACTACATGG CAGATGCCAA GACCATCGTC 
AATGTGCAAA CCATGTTCCG GCTTTTCCCC AGTCTCAGCA TCACCACGGA GCTCACACAC 
CCTTCCAACA TGCGGTTCAT GCAGTTCCGT GCCAAGGACA GCTACTCTCT GGCTCTTTCC 
AAACTTGAAA AGCAAGAACG GGAGAACGGC TCCAACCTGG CCTTCATGTT CCGCCTGCCA 
TTTGCTGCTG GTCGAGTATT TAGTATCAGC ATGTTGGATA CACTGCTCTA CCAGTCCTTC 
GTGAAGGACT ACATGATCAC CATCACCAGG CTGCTCTTGG GCCTTGATAC TACACCAGGC 
TCCGGCTACC TCTGTGCAAT GAAGGTAACC GAGGACGACC TGTGGATCCG CACTTACGGC 
CGCCTCTTCC AGAAACTCTG CTCCTCCAGC GCCGAGATCC CCATCGGCAT CTACAGGACC 
GAGTGCCATG TCTTCTCGGA GCCCCATGAC GTCAGAGCCC AGGGGGGCCC AGACATAGCC 
CTCCTGCCTG CCCAGTCTCA GATCTCGGTG AACATGGAGG ACTGCGAGGA TACTCGGGAG 
GCCAAGGGAC CCTGGGGCAC ACGAGCTGCA TCTGGCAGTG GCAGCACCCA TGGCCGTCAC 
GGGGGCAGTG CTGACCCAGT GGAGCACCCA CTACTACGTC GCAAGAGCCT GCAGTGGGCC 
CGCAAGCTGA GTCGCAAGAG CACCAAGCAG GCAGGGAAGG CACCTGTGGC CACAGACTGG 
ATCACCCAGC AGCGGCTCAG CCTGTACCGA CGCTCAGAGC GCCAGGAGCT CTCAGAGCTG 
GTCAAGAACC GAATGAAGCA CCTGGGACTG CCCACCACTG GCTATGAGGA CGTAGCAAAT 
TTAACAGCCA GTGATGTGAT GAATCGGGTA AACCTGGGAT ATTTGCAAGA TGAGATGAAC 
GATCATCACC AGAACACCCT TTCCTATGTA CTCATCAACC CCCCGCCAGA CACAAGACTG 
GAACCCAACG ACATTGTGTA CCTCATCCGT TCCGACCCCC TGGCCCATGT GGCCAGCAGC 
TCCCAGAGTC GGAAAAGCAG CTGCAGCAAC AAGCTCTCAT CCTGTAATCC TGAGACCAGG 
GATGAGACCC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_017317196.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172685.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X11, mRNA.

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CloneID	OMu80186
Clone ID Related Accession (Same CDS sequence)	XM_017317196.1
Accession Version	XM_017317196.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGT CCTTCGTGAA GGACTACATG ATCACCATCA CCAGGCTGCT CTTGGGCCTT 
GATACTACAC CAGGCTCCGG CTACCTCTGT GCAATGAAGG TAACCGAGGA CGACCTGTGG 
ATCCGCACTT ACGGCCGCCT CTTCCAGAAA CTCTGCTCCT CCAGCGCCGA GATCCCCATC 
GGCATCTACA GGACCGAGTG CCATGTCTTC TCGGAGCCCC ATGACGTCAG AGCCCAGTCT 
CAGATCTCGG TGAACATGGA GGACTGCGAG GATACTCGGG AGGCCAAGGG ACCCTGGGGC 
ACACGAGCTG CATCTGGCAG TGGCAGCACC CATGGCCGTC ACGGGGGCAG TGCTGACCCA 
GTGGAGCACC CACTACTACG TCGCAAGAGC CTGCAGTGGG CCCGCAAGCT GAGTCGCAAG 
AGCACCAAGC AGGCAGGGAA GGCACCTGTG GCCACAGACT GGATCACCCA GCAGCGGCTC 
AGCCTGTACC GACGCTCAGA GCGCCAGGAG CTCTCAGAGC TGGTCAAGAA CCGAATGAAG 
CACCTGGGAC TGCCCACCAC TGGCTATGAT GAGATGAACG ATCATCACCA GAACACCCTT 
TCCTATGTAC TCATCAACCC CCCGCCAGAC ACAAGACTGG AACCCAACGA CATTGTGTAC 
CTCATCCGTT CCGACCCCCT GGCCCATGTG GCCAGCAGCT CCCAGAGTCG GAAAAGCAGC 
TGCAGCAACA AGCTCTCATC CTGTAATCCT GAGACCAGGG ATGAGACCCA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172685.1
CDS	202..3858
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_017317196.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317196.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGT CCTTCGTGAA GGACTACATG ATCACCATCA CCAGGCTGCT CTTGGGCCTT 
GATACTACAC CAGGCTCCGG CTACCTCTGT GCAATGAAGG TAACCGAGGA CGACCTGTGG 
ATCCGCACTT ACGGCCGCCT CTTCCAGAAA CTCTGCTCCT CCAGCGCCGA GATCCCCATC 
GGCATCTACA GGACCGAGTG CCATGTCTTC TCGGAGCCCC ATGACGTCAG AGCCCAGTCT 
CAGATCTCGG TGAACATGGA GGACTGCGAG GATACTCGGG AGGCCAAGGG ACCCTGGGGC 
ACACGAGCTG CATCTGGCAG TGGCAGCACC CATGGCCGTC ACGGGGGCAG TGCTGACCCA 
GTGGAGCACC CACTACTACG TCGCAAGAGC CTGCAGTGGG CCCGCAAGCT GAGTCGCAAG 
AGCACCAAGC AGGCAGGGAA GGCACCTGTG GCCACAGACT GGATCACCCA GCAGCGGCTC 
AGCCTGTACC GACGCTCAGA GCGCCAGGAG CTCTCAGAGC TGGTCAAGAA CCGAATGAAG 
CACCTGGGAC TGCCCACCAC TGGCTATGAT GAGATGAACG ATCATCACCA GAACACCCTT 
TCCTATGTAC TCATCAACCC CCCGCCAGAC ACAAGACTGG AACCCAACGA CATTGTGTAC 
CTCATCCGTT CCGACCCCCT GGCCCATGTG GCCAGCAGCT CCCAGAGTCG GAAAAGCAGC 
TGCAGCAACA AGCTCTCATC CTGTAATCCT GAGACCAGGG ATGAGACCCA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_017317197.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172686.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X12, mRNA.

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CloneID	OMu80187
Clone ID Related Accession (Same CDS sequence)	XM_017317197.1 , XM_017317197.2
Accession Version	XM_017317197.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGTTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTA CTGCAGTGTG GCATCATCTA TGCTGACAAC 
CTGGTGGTGG TGGACAAGGA GAGCACTATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATCG TCAATGTGCA AACCATGTTC CGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACAC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GTGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGAGTA TTTAGTATCA GCATGTTGGA TACACTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTCTT GGGCCTTGAT 
ACTACACCAG GCTCCGGCTA CCTCTGTGCA ATGAAGGTAA CCGAGGACGA CCTGTGGATC 
CGCACTTACG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCCGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCG GAGCCCCATG ACGTCAGAGC CCAGTCTCAG 
ATCTCGGTGA ACATGGAGGA CTGCGAGGAT ACTCGGGAGG CCAAGGGACC CTGGGGCACA 
CGAGCTGCAT CTGGCAGTGG CAGCACCCAT GGCCGTCACG GGGGCAGTGC TGACCCAGTG 
GAGCACCCAC TACTACGTCG CAAGAGCCTG CAGTGGGCCC GCAAGCTGAG TCGCAAGAGC 
ACCAAGCAGG CAGGGAAGGC ACCTGTGGCC ACAGACTGGA TCACCCAGCA GCGGCTCAGC 
CTGTACCGAC GCTCAGAGCG CCAGGAGCTC TCAGAGCTGG TCAAGAACCG AATGAAGCAC 
CTGGGACTGC CCACCACTGG CTATGATGAG ATGAACGATC ATCACCAGAA CACCCTTTCC 
TATGTACTCA TCAACCCCCC GCCAGACACA AGACTGGAAC CCAACGACAT TGTGTACCTC 
ATCCGTTCCG ACCCCCTGGC CCATGTGGCC AGCAGCTCCC AGAGTCGGAA AAGCAGCTGC 
AGCAACAAGC TCTCATCCTG TAATCCTGAG ACCAGGGATG AGACCCAGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172686.1
CDS	59..3712
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_017317197.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317197.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGTTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTA CTGCAGTGTG GCATCATCTA TGCTGACAAC 
CTGGTGGTGG TGGACAAGGA GAGCACTATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATCG TCAATGTGCA AACCATGTTC CGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACAC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GTGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGAGTA TTTAGTATCA GCATGTTGGA TACACTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTCTT GGGCCTTGAT 
ACTACACCAG GCTCCGGCTA CCTCTGTGCA ATGAAGGTAA CCGAGGACGA CCTGTGGATC 
CGCACTTACG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCCGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCG GAGCCCCATG ACGTCAGAGC CCAGTCTCAG 
ATCTCGGTGA ACATGGAGGA CTGCGAGGAT ACTCGGGAGG CCAAGGGACC CTGGGGCACA 
CGAGCTGCAT CTGGCAGTGG CAGCACCCAT GGCCGTCACG GGGGCAGTGC TGACCCAGTG 
GAGCACCCAC TACTACGTCG CAAGAGCCTG CAGTGGGCCC GCAAGCTGAG TCGCAAGAGC 
ACCAAGCAGG CAGGGAAGGC ACCTGTGGCC ACAGACTGGA TCACCCAGCA GCGGCTCAGC 
CTGTACCGAC GCTCAGAGCG CCAGGAGCTC TCAGAGCTGG TCAAGAACCG AATGAAGCAC 
CTGGGACTGC CCACCACTGG CTATGATGAG ATGAACGATC ATCACCAGAA CACCCTTTCC 
TATGTACTCA TCAACCCCCC GCCAGACACA AGACTGGAAC CCAACGACAT TGTGTACCTC 
ATCCGTTCCG ACCCCCTGGC CCATGTGGCC AGCAGCTCCC AGAGTCGGAA AAGCAGCTGC 
AGCAACAAGC TCTCATCCTG TAATCCTGAG ACCAGGGATG AGACCCAGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_017317197.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172686.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X13, mRNA.

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CloneID	OMu80187
Clone ID Related Accession (Same CDS sequence)	XM_017317197.1 , XM_017317197.2
Accession Version	XM_017317197.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGTTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTA CTGCAGTGTG GCATCATCTA TGCTGACAAC 
CTGGTGGTGG TGGACAAGGA GAGCACTATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATCG TCAATGTGCA AACCATGTTC CGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACAC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GTGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGAGTA TTTAGTATCA GCATGTTGGA TACACTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTCTT GGGCCTTGAT 
ACTACACCAG GCTCCGGCTA CCTCTGTGCA ATGAAGGTAA CCGAGGACGA CCTGTGGATC 
CGCACTTACG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCCGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCG GAGCCCCATG ACGTCAGAGC CCAGTCTCAG 
ATCTCGGTGA ACATGGAGGA CTGCGAGGAT ACTCGGGAGG CCAAGGGACC CTGGGGCACA 
CGAGCTGCAT CTGGCAGTGG CAGCACCCAT GGCCGTCACG GGGGCAGTGC TGACCCAGTG 
GAGCACCCAC TACTACGTCG CAAGAGCCTG CAGTGGGCCC GCAAGCTGAG TCGCAAGAGC 
ACCAAGCAGG CAGGGAAGGC ACCTGTGGCC ACAGACTGGA TCACCCAGCA GCGGCTCAGC 
CTGTACCGAC GCTCAGAGCG CCAGGAGCTC TCAGAGCTGG TCAAGAACCG AATGAAGCAC 
CTGGGACTGC CCACCACTGG CTATGATGAG ATGAACGATC ATCACCAGAA CACCCTTTCC 
TATGTACTCA TCAACCCCCC GCCAGACACA AGACTGGAAC CCAACGACAT TGTGTACCTC 
ATCCGTTCCG ACCCCCTGGC CCATGTGGCC AGCAGCTCCC AGAGTCGGAA AAGCAGCTGC 
AGCAACAAGC TCTCATCCTG TAATCCTGAG ACCAGGGATG AGACCCAGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172686.1
CDS	59..3712
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_017317197.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317197.2

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGTTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTA CTGCAGTGTG GCATCATCTA TGCTGACAAC 
CTGGTGGTGG TGGACAAGGA GAGCACTATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATCG TCAATGTGCA AACCATGTTC CGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACAC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GTGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGAGTA TTTAGTATCA GCATGTTGGA TACACTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTCTT GGGCCTTGAT 
ACTACACCAG GCTCCGGCTA CCTCTGTGCA ATGAAGGTAA CCGAGGACGA CCTGTGGATC 
CGCACTTACG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCCGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCG GAGCCCCATG ACGTCAGAGC CCAGTCTCAG 
ATCTCGGTGA ACATGGAGGA CTGCGAGGAT ACTCGGGAGG CCAAGGGACC CTGGGGCACA 
CGAGCTGCAT CTGGCAGTGG CAGCACCCAT GGCCGTCACG GGGGCAGTGC TGACCCAGTG 
GAGCACCCAC TACTACGTCG CAAGAGCCTG CAGTGGGCCC GCAAGCTGAG TCGCAAGAGC 
ACCAAGCAGG CAGGGAAGGC ACCTGTGGCC ACAGACTGGA TCACCCAGCA GCGGCTCAGC 
CTGTACCGAC GCTCAGAGCG CCAGGAGCTC TCAGAGCTGG TCAAGAACCG AATGAAGCAC 
CTGGGACTGC CCACCACTGG CTATGATGAG ATGAACGATC ATCACCAGAA CACCCTTTCC 
TATGTACTCA TCAACCCCCC GCCAGACACA AGACTGGAAC CCAACGACAT TGTGTACCTC 
ATCCGTTCCG ACCCCCTGGC CCATGTGGCC AGCAGCTCCC AGAGTCGGAA AAGCAGCTGC 
AGCAACAAGC TCTCATCCTG TAATCCTGAG ACCAGGGATG AGACCCAGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_017317198.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172687.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X14, mRNA.

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CloneID	OMu80188
Clone ID Related Accession (Same CDS sequence)	XM_017317198.2 , XM_017317198.1
Accession Version	XM_017317198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGGGAA GGACAGGAGT CTCCCGAGCA GTGGCAGCGC ACGTACGGGA 
GGTGCTCGGG CAACGAGGTG TACCACATTC GCATGGGTGA CAGTAAATTC TTCCGGGAGT 
ATGAGGGCAA GAGCTTCACC TACGCAGCCT TCCACGCGCA CAAGAAGGAC AGTGGCCATG 
GACCTGCAGA ACACAGATTG CCGGCCCTCC CAGGGTGGCA GTGGCGGGGA CGGCACAAAG 
CTGACTCTGC CCACCGAGAA CGGCTCTGGT AGTCGACGCC CCAGCATCGC ACCCGTTCTG 
GAGTTGGCAG ACAGCTCAGC CCTGTTGCCC TGCGACCTGC TGAGTGACCA GTCAGAGGAT 
GAGGTGACAC CCTCGGACGA CGAGGGGCTC TCTGTGGTTG AGTACGTGAA GGGCTATCCC 
CCCAACTCAC CCTACATTGG CAGCTCCCCG ACTTTATGCC ACCTCCTGCC TGTGAAAGCC 
CCCTTCTGCT GCCTGCGGTT GGACAAGGGC TGCAAACACA ACAGCTACGA GGATGCCAAG 
GCCTATGGGT TCAAGAACAA GCTGATTATT GTCTCTGCTG AGACGGCAGG CAACGGGCTC 
TACAACTTCA TCGTGCCTCT GCGCGCCTAC TACCGGTCCC GCCGGGAGCT CAACCCTATC 
GTGTTGCTGC TTGACAACAA GCCTGACCAC CACTTCCTGG AGGCCATCTG TTGCTTCCCC 
ATGGTCTACT ACATGGAGGG ATCCGTGGAC AACCTGGACA GCTTACTGCA GTGTGGCATC 
ATCTATGCTG ACAACCTGGT GGTGGTGGAC AAGGAGAGCA CTATGAGCGC TGAAGAGGAC 
TACATGGCAG ATGCCAAGAC CATCGTCAAT GTGCAAACCA TGTTCCGGCT TTTCCCCAGT 
CTCAGCATCA CCACGGAGCT CACACACCCT TCCAACATGC GGTTCATGCA GTTCCGTGCC 
AAGGACAGCT ACTCTCTGGC TCTTTCCAAA CTTGAAAAGC AAGAACGGGA GAACGGCTCC 
AACCTGGCCT TCATGTTCCG CCTGCCATTT GCTGCTGGTC GAGTATTTAG TATCAGCATG 
TTGGATACAC TGCTCTACCA GTCCTTCGTG AAGGACTACA TGATCACCAT CACCAGGCTG 
CTCTTGGGCC TTGATACTAC ACCAGGCTCC GGCTACCTCT GTGCAATGAA GGTAACCGAG 
GACGACCTGT GGATCCGCAC TTACGGCCGC CTCTTCCAGA AACTCTGCTC CTCCAGCGCC 
GAGATCCCCA TCGGCATCTA CAGGACCGAG TGCCATGTCT TCTCGGAGCC CCATGACGTC 
AGAGCCCAGG GGGGCCCAGA CATAGCCCTC CTGCCTGCCC AGTCTCAGAT CTCGGTGAAC 
ATGGAGGACT GCGAGGATAC TCGGGAGGCC AAGGGACCCT GGGGCACACG AGCTGCATCT 
GGCAGTGGCA GCACCCATGG CCGTCACGGG GGCAGTGCTG ACCCAGTGGA GCACCCACTA 
CTACGTCGCA AGAGCCTGCA GTGGGCCCGC AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA 
GGGAAGGCAC CTGTGGCCAC AGACTGGATC ACCCAGCAGC GGCTCAGCCT GTACCGACGC 
TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC AAGAACCGAA TGAAGCACCT GGGACTGCCC 
ACCACTGGCT ATGAGGACGT AGCAAATTTA ACAGCCAGTG ATGTGATGAA TCGGGTAAAC 
CTGGGATATT TGCAAGATGA GATGAACGAT CATCACCAGA ACACCCTTTC CTATGTACTC 
ATCAACCCCC CGCCAGACAC AAGACTGGAA CCCAACGACA TTGTGTACCT CATCCGTTCC 
GACCCCCTGG CCCATGTGGC CAGCAGCTCC CAGAGTCGGA AAAGCAGCTG CAGCAACAAG 
CTCTCATCCT GTAATCCTGA GACCAGGGAT GAGACCCAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172687.1
CDS	200..3604
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAGKDRSLPSSGSARTGGARATRCTTFAWVTVNSSGSMRARASPTQPSTRTRRTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_017317198.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317198.2

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGGGAA GGACAGGAGT CTCCCGAGCA GTGGCAGCGC ACGTACGGGA 
GGTGCTCGGG CAACGAGGTG TACCACATTC GCATGGGTGA CAGTAAATTC TTCCGGGAGT 
ATGAGGGCAA GAGCTTCACC TACGCAGCCT TCCACGCGCA CAAGAAGGAC AGTGGCCATG 
GACCTGCAGA ACACAGATTG CCGGCCCTCC CAGGGTGGCA GTGGCGGGGA CGGCACAAAG 
CTGACTCTGC CCACCGAGAA CGGCTCTGGT AGTCGACGCC CCAGCATCGC ACCCGTTCTG 
GAGTTGGCAG ACAGCTCAGC CCTGTTGCCC TGCGACCTGC TGAGTGACCA GTCAGAGGAT 
GAGGTGACAC CCTCGGACGA CGAGGGGCTC TCTGTGGTTG AGTACGTGAA GGGCTATCCC 
CCCAACTCAC CCTACATTGG CAGCTCCCCG ACTTTATGCC ACCTCCTGCC TGTGAAAGCC 
CCCTTCTGCT GCCTGCGGTT GGACAAGGGC TGCAAACACA ACAGCTACGA GGATGCCAAG 
GCCTATGGGT TCAAGAACAA GCTGATTATT GTCTCTGCTG AGACGGCAGG CAACGGGCTC 
TACAACTTCA TCGTGCCTCT GCGCGCCTAC TACCGGTCCC GCCGGGAGCT CAACCCTATC 
GTGTTGCTGC TTGACAACAA GCCTGACCAC CACTTCCTGG AGGCCATCTG TTGCTTCCCC 
ATGGTCTACT ACATGGAGGG ATCCGTGGAC AACCTGGACA GCTTACTGCA GTGTGGCATC 
ATCTATGCTG ACAACCTGGT GGTGGTGGAC AAGGAGAGCA CTATGAGCGC TGAAGAGGAC 
TACATGGCAG ATGCCAAGAC CATCGTCAAT GTGCAAACCA TGTTCCGGCT TTTCCCCAGT 
CTCAGCATCA CCACGGAGCT CACACACCCT TCCAACATGC GGTTCATGCA GTTCCGTGCC 
AAGGACAGCT ACTCTCTGGC TCTTTCCAAA CTTGAAAAGC AAGAACGGGA GAACGGCTCC 
AACCTGGCCT TCATGTTCCG CCTGCCATTT GCTGCTGGTC GAGTATTTAG TATCAGCATG 
TTGGATACAC TGCTCTACCA GTCCTTCGTG AAGGACTACA TGATCACCAT CACCAGGCTG 
CTCTTGGGCC TTGATACTAC ACCAGGCTCC GGCTACCTCT GTGCAATGAA GGTAACCGAG 
GACGACCTGT GGATCCGCAC TTACGGCCGC CTCTTCCAGA AACTCTGCTC CTCCAGCGCC 
GAGATCCCCA TCGGCATCTA CAGGACCGAG TGCCATGTCT TCTCGGAGCC CCATGACGTC 
AGAGCCCAGG GGGGCCCAGA CATAGCCCTC CTGCCTGCCC AGTCTCAGAT CTCGGTGAAC 
ATGGAGGACT GCGAGGATAC TCGGGAGGCC AAGGGACCCT GGGGCACACG AGCTGCATCT 
GGCAGTGGCA GCACCCATGG CCGTCACGGG GGCAGTGCTG ACCCAGTGGA GCACCCACTA 
CTACGTCGCA AGAGCCTGCA GTGGGCCCGC AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA 
GGGAAGGCAC CTGTGGCCAC AGACTGGATC ACCCAGCAGC GGCTCAGCCT GTACCGACGC 
TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC AAGAACCGAA TGAAGCACCT GGGACTGCCC 
ACCACTGGCT ATGAGGACGT AGCAAATTTA ACAGCCAGTG ATGTGATGAA TCGGGTAAAC 
CTGGGATATT TGCAAGATGA GATGAACGAT CATCACCAGA ACACCCTTTC CTATGTACTC 
ATCAACCCCC CGCCAGACAC AAGACTGGAA CCCAACGACA TTGTGTACCT CATCCGTTCC 
GACCCCCTGG CCCATGTGGC CAGCAGCTCC CAGAGTCGGA AAAGCAGCTG CAGCAACAAG 
CTCTCATCCT GTAATCCTGA GACCAGGGAT GAGACCCAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_017317198.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172687.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu80188
Clone ID Related Accession (Same CDS sequence)	XM_017317198.2 , XM_017317198.1
Accession Version	XM_017317198.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGGGAA GGACAGGAGT CTCCCGAGCA GTGGCAGCGC ACGTACGGGA 
GGTGCTCGGG CAACGAGGTG TACCACATTC GCATGGGTGA CAGTAAATTC TTCCGGGAGT 
ATGAGGGCAA GAGCTTCACC TACGCAGCCT TCCACGCGCA CAAGAAGGAC AGTGGCCATG 
GACCTGCAGA ACACAGATTG CCGGCCCTCC CAGGGTGGCA GTGGCGGGGA CGGCACAAAG 
CTGACTCTGC CCACCGAGAA CGGCTCTGGT AGTCGACGCC CCAGCATCGC ACCCGTTCTG 
GAGTTGGCAG ACAGCTCAGC CCTGTTGCCC TGCGACCTGC TGAGTGACCA GTCAGAGGAT 
GAGGTGACAC CCTCGGACGA CGAGGGGCTC TCTGTGGTTG AGTACGTGAA GGGCTATCCC 
CCCAACTCAC CCTACATTGG CAGCTCCCCG ACTTTATGCC ACCTCCTGCC TGTGAAAGCC 
CCCTTCTGCT GCCTGCGGTT GGACAAGGGC TGCAAACACA ACAGCTACGA GGATGCCAAG 
GCCTATGGGT TCAAGAACAA GCTGATTATT GTCTCTGCTG AGACGGCAGG CAACGGGCTC 
TACAACTTCA TCGTGCCTCT GCGCGCCTAC TACCGGTCCC GCCGGGAGCT CAACCCTATC 
GTGTTGCTGC TTGACAACAA GCCTGACCAC CACTTCCTGG AGGCCATCTG TTGCTTCCCC 
ATGGTCTACT ACATGGAGGG ATCCGTGGAC AACCTGGACA GCTTACTGCA GTGTGGCATC 
ATCTATGCTG ACAACCTGGT GGTGGTGGAC AAGGAGAGCA CTATGAGCGC TGAAGAGGAC 
TACATGGCAG ATGCCAAGAC CATCGTCAAT GTGCAAACCA TGTTCCGGCT TTTCCCCAGT 
CTCAGCATCA CCACGGAGCT CACACACCCT TCCAACATGC GGTTCATGCA GTTCCGTGCC 
AAGGACAGCT ACTCTCTGGC TCTTTCCAAA CTTGAAAAGC AAGAACGGGA GAACGGCTCC 
AACCTGGCCT TCATGTTCCG CCTGCCATTT GCTGCTGGTC GAGTATTTAG TATCAGCATG 
TTGGATACAC TGCTCTACCA GTCCTTCGTG AAGGACTACA TGATCACCAT CACCAGGCTG 
CTCTTGGGCC TTGATACTAC ACCAGGCTCC GGCTACCTCT GTGCAATGAA GGTAACCGAG 
GACGACCTGT GGATCCGCAC TTACGGCCGC CTCTTCCAGA AACTCTGCTC CTCCAGCGCC 
GAGATCCCCA TCGGCATCTA CAGGACCGAG TGCCATGTCT TCTCGGAGCC CCATGACGTC 
AGAGCCCAGG GGGGCCCAGA CATAGCCCTC CTGCCTGCCC AGTCTCAGAT CTCGGTGAAC 
ATGGAGGACT GCGAGGATAC TCGGGAGGCC AAGGGACCCT GGGGCACACG AGCTGCATCT 
GGCAGTGGCA GCACCCATGG CCGTCACGGG GGCAGTGCTG ACCCAGTGGA GCACCCACTA 
CTACGTCGCA AGAGCCTGCA GTGGGCCCGC AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA 
GGGAAGGCAC CTGTGGCCAC AGACTGGATC ACCCAGCAGC GGCTCAGCCT GTACCGACGC 
TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC AAGAACCGAA TGAAGCACCT GGGACTGCCC 
ACCACTGGCT ATGAGGACGT AGCAAATTTA ACAGCCAGTG ATGTGATGAA TCGGGTAAAC 
CTGGGATATT TGCAAGATGA GATGAACGAT CATCACCAGA ACACCCTTTC CTATGTACTC 
ATCAACCCCC CGCCAGACAC AAGACTGGAA CCCAACGACA TTGTGTACCT CATCCGTTCC 
GACCCCCTGG CCCATGTGGC CAGCAGCTCC CAGAGTCGGA AAAGCAGCTG CAGCAACAAG 
CTCTCATCCT GTAATCCTGA GACCAGGGAT GAGACCCAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172687.1
CDS	202..3606
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAGKDRSLPSSGSARTGGARATRCTTFAWVTVNSSGSMRARASPTQPSTRTRRTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_017317198.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317198.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGGGAA GGACAGGAGT CTCCCGAGCA GTGGCAGCGC ACGTACGGGA 
GGTGCTCGGG CAACGAGGTG TACCACATTC GCATGGGTGA CAGTAAATTC TTCCGGGAGT 
ATGAGGGCAA GAGCTTCACC TACGCAGCCT TCCACGCGCA CAAGAAGGAC AGTGGCCATG 
GACCTGCAGA ACACAGATTG CCGGCCCTCC CAGGGTGGCA GTGGCGGGGA CGGCACAAAG 
CTGACTCTGC CCACCGAGAA CGGCTCTGGT AGTCGACGCC CCAGCATCGC ACCCGTTCTG 
GAGTTGGCAG ACAGCTCAGC CCTGTTGCCC TGCGACCTGC TGAGTGACCA GTCAGAGGAT 
GAGGTGACAC CCTCGGACGA CGAGGGGCTC TCTGTGGTTG AGTACGTGAA GGGCTATCCC 
CCCAACTCAC CCTACATTGG CAGCTCCCCG ACTTTATGCC ACCTCCTGCC TGTGAAAGCC 
CCCTTCTGCT GCCTGCGGTT GGACAAGGGC TGCAAACACA ACAGCTACGA GGATGCCAAG 
GCCTATGGGT TCAAGAACAA GCTGATTATT GTCTCTGCTG AGACGGCAGG CAACGGGCTC 
TACAACTTCA TCGTGCCTCT GCGCGCCTAC TACCGGTCCC GCCGGGAGCT CAACCCTATC 
GTGTTGCTGC TTGACAACAA GCCTGACCAC CACTTCCTGG AGGCCATCTG TTGCTTCCCC 
ATGGTCTACT ACATGGAGGG ATCCGTGGAC AACCTGGACA GCTTACTGCA GTGTGGCATC 
ATCTATGCTG ACAACCTGGT GGTGGTGGAC AAGGAGAGCA CTATGAGCGC TGAAGAGGAC 
TACATGGCAG ATGCCAAGAC CATCGTCAAT GTGCAAACCA TGTTCCGGCT TTTCCCCAGT 
CTCAGCATCA CCACGGAGCT CACACACCCT TCCAACATGC GGTTCATGCA GTTCCGTGCC 
AAGGACAGCT ACTCTCTGGC TCTTTCCAAA CTTGAAAAGC AAGAACGGGA GAACGGCTCC 
AACCTGGCCT TCATGTTCCG CCTGCCATTT GCTGCTGGTC GAGTATTTAG TATCAGCATG 
TTGGATACAC TGCTCTACCA GTCCTTCGTG AAGGACTACA TGATCACCAT CACCAGGCTG 
CTCTTGGGCC TTGATACTAC ACCAGGCTCC GGCTACCTCT GTGCAATGAA GGTAACCGAG 
GACGACCTGT GGATCCGCAC TTACGGCCGC CTCTTCCAGA AACTCTGCTC CTCCAGCGCC 
GAGATCCCCA TCGGCATCTA CAGGACCGAG TGCCATGTCT TCTCGGAGCC CCATGACGTC 
AGAGCCCAGG GGGGCCCAGA CATAGCCCTC CTGCCTGCCC AGTCTCAGAT CTCGGTGAAC 
ATGGAGGACT GCGAGGATAC TCGGGAGGCC AAGGGACCCT GGGGCACACG AGCTGCATCT 
GGCAGTGGCA GCACCCATGG CCGTCACGGG GGCAGTGCTG ACCCAGTGGA GCACCCACTA 
CTACGTCGCA AGAGCCTGCA GTGGGCCCGC AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA 
GGGAAGGCAC CTGTGGCCAC AGACTGGATC ACCCAGCAGC GGCTCAGCCT GTACCGACGC 
TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC AAGAACCGAA TGAAGCACCT GGGACTGCCC 
ACCACTGGCT ATGAGGACGT AGCAAATTTA ACAGCCAGTG ATGTGATGAA TCGGGTAAAC 
CTGGGATATT TGCAAGATGA GATGAACGAT CATCACCAGA ACACCCTTTC CTATGTACTC 
ATCAACCCCC CGCCAGACAC AAGACTGGAA CCCAACGACA TTGTGTACCT CATCCGTTCC 
GACCCCCTGG CCCATGTGGC CAGCAGCTCC CAGAGTCGGA AAAGCAGCTG CAGCAACAAG 
CTCTCATCCT GTAATCCTGA GACCAGGGAT GAGACCCAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_017317199.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172688.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OMu80189
Clone ID Related Accession (Same CDS sequence)	XM_017317199.1
Accession Version	XM_017317199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC 
GGGAGGTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG 
TGCCTCATCG GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG 
CACATCCTGG CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA 
TATGAAGGGC CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA 
AAGCTGACTC TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GATGAGGTGA CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT 
CCCCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC 
AAGGCCTATG GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT 
ATCGTGTTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGTTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTACT GCAGTGTGGC 
ATCATCTATG CTGACAACCT GGTGGTGGTG GACAAGGAGA GCACTATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATCGTC AATGTGCAAA CCATGTTCCG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACACAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGT 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGAGTATT TAGTATCAGC 
ATGTTGGATA CACTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTCTTGG GCCTTGATAC TACACCAGGC TCCGGCTACC TCTGTGCAAT GAAGGTAACC 
GAGGACGACC TGTGGATCCG CACTTACGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCCGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCGGA GCCCCATGAC 
GTCAGAGCCC AGTCTCAGAT CTCGGTGAAC ATGGAGGACT GCGAGGATAC TCGGGAGGCC 
AAGGGACCCT GGGGCACACG AGCTGCATCT GGCAGTGGCA GCACCCATGG CCGTCACGGG 
GGCAGTGCTG ACCCAGTGGA GCACCCACTA CTACGTCGCA AGAGCCTGCA GTGGGCCCGC 
AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA GGGAAGGCAC CTGTGGCCAC AGACTGGATC 
ACCCAGCAGC GGCTCAGCCT GTACCGACGC TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGAA TGAAGCACCT GGGACTGCCC ACCACTGGCT ATGATGAGAT GAACGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAACCC 
AACGACATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAGCTC TCATCCTGTA ATCCTGAGAC CAGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172688.1
CDS	2420..5611
Translation	MALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_017317199.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317199.1

ORF Insert Sequence:

ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC 
GGGAGGTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG 
TGCCTCATCG GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG 
CACATCCTGG CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA 
TATGAAGGGC CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA 
AAGCTGACTC TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GATGAGGTGA CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT 
CCCCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC 
AAGGCCTATG GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT 
ATCGTGTTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGTTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTACT GCAGTGTGGC 
ATCATCTATG CTGACAACCT GGTGGTGGTG GACAAGGAGA GCACTATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATCGTC AATGTGCAAA CCATGTTCCG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACACAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGT 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGAGTATT TAGTATCAGC 
ATGTTGGATA CACTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTCTTGG GCCTTGATAC TACACCAGGC TCCGGCTACC TCTGTGCAAT GAAGGTAACC 
GAGGACGACC TGTGGATCCG CACTTACGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCCGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCGGA GCCCCATGAC 
GTCAGAGCCC AGTCTCAGAT CTCGGTGAAC ATGGAGGACT GCGAGGATAC TCGGGAGGCC 
AAGGGACCCT GGGGCACACG AGCTGCATCT GGCAGTGGCA GCACCCATGG CCGTCACGGG 
GGCAGTGCTG ACCCAGTGGA GCACCCACTA CTACGTCGCA AGAGCCTGCA GTGGGCCCGC 
AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA GGGAAGGCAC CTGTGGCCAC AGACTGGATC 
ACCCAGCAGC GGCTCAGCCT GTACCGACGC TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGAA TGAAGCACCT GGGACTGCCC ACCACTGGCT ATGATGAGAT GAACGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAACCC 
AACGACATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAGCTC TCATCCTGTA ATCCTGAGAC CAGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_030249645.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105505.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu87787
Clone ID Related Accession (Same CDS sequence)	XM_030249645.1
Accession Version	XM_030249645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC 
GGGAGGTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG 
TGCCTCATCG GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG 
CACATCCTGG CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA 
TATGAAGGGC CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA 
AAGCTGACTC TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GATGAGGTGA CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT 
CCCCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC 
AAGGCCTATG GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT 
ATCGTGTTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGTTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTACT GCAGTGTGGC 
ATCATCTATG CTGACAACCT GGTGGTGGTG GACAAGGAGA GCACTATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATCGTC AATGTGCAAA CCATGTTCCG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACACAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGT 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGAGTATT TAGTATCAGC 
ATGTTGGATA CACTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTCTTGG GCCTTGATAC TACACCAGGC TCCGGCTACC TCTGTGCAAT GAAGGTAACC 
GAGGACGACC TGTGGATCCG CACTTACGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCCGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCGGA GCCCCATGAC 
GTCAGAGCCC AGGGGGGCCC AGACATAGCC CTCCTGCCTG CCCAGTCTCA GATCTCGGTG 
AACATGGAGG ACTGCGAGGA TACTCGGGAG GCCAAGGGAC CCTGGGGCAC ACGAGCTGCA 
TCTGGCAGTG GCAGCACCCA TGGCCGTCAC GGGGGCAGTG CTGACCCAGT GGAGCACCCA 
CTACTACGTC GCAAGAGCCT GCAGTGGGCC CGCAAGCTGA GTCGCAAGAG CACCAAGCAG 
GCAGGGAAGG CACCTGTGGC CACAGACTGG ATCACCCAGC AGCGGCTCAG CCTGTACCGA 
CGCTCAGAGC GCCAGGAGCT CTCAGAGCTG GTCAAGAACC GAATGAAGCA CCTGGGACTG 
CCCACCACTG GCTATGAGGA CGTAGCAAAT TTAACAGCCA GTGATGTGAT GAATCGGGTA 
AACCTGGGAT ATTTGCAAGA TGAGATGAAC GATCATCACC AGAACACCCT TTCCTATGTA 
CTCATCAACC CCCCGCCAGA CACAAGACTG GAACCCAACG ACATTGTGTA CCTCATCCGT 
TCCGACCCCC TGGCCCATGT GGCCAGCAGC TCCCAGAGTC GGAAAAGCAG CTGCAGCAAC 
AAGCTCTCAT CCTGTAATCC TGAGACCAGG GATGAGACCC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105505.1
CDS	57..3764
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_030249645.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249645.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC 
GGGAGGTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG 
TGCCTCATCG GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG 
CACATCCTGG CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA 
TATGAAGGGC CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA 
AAGCTGACTC TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GATGAGGTGA CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT 
CCCCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC 
AAGGCCTATG GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT 
ATCGTGTTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGTTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTACT GCAGTGTGGC 
ATCATCTATG CTGACAACCT GGTGGTGGTG GACAAGGAGA GCACTATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATCGTC AATGTGCAAA CCATGTTCCG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACACAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGT 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGAGTATT TAGTATCAGC 
ATGTTGGATA CACTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTCTTGG GCCTTGATAC TACACCAGGC TCCGGCTACC TCTGTGCAAT GAAGGTAACC 
GAGGACGACC TGTGGATCCG CACTTACGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCCGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCGGA GCCCCATGAC 
GTCAGAGCCC AGGGGGGCCC AGACATAGCC CTCCTGCCTG CCCAGTCTCA GATCTCGGTG 
AACATGGAGG ACTGCGAGGA TACTCGGGAG GCCAAGGGAC CCTGGGGCAC ACGAGCTGCA 
TCTGGCAGTG GCAGCACCCA TGGCCGTCAC GGGGGCAGTG CTGACCCAGT GGAGCACCCA 
CTACTACGTC GCAAGAGCCT GCAGTGGGCC CGCAAGCTGA GTCGCAAGAG CACCAAGCAG 
GCAGGGAAGG CACCTGTGGC CACAGACTGG ATCACCCAGC AGCGGCTCAG CCTGTACCGA 
CGCTCAGAGC GCCAGGAGCT CTCAGAGCTG GTCAAGAACC GAATGAAGCA CCTGGGACTG 
CCCACCACTG GCTATGAGGA CGTAGCAAAT TTAACAGCCA GTGATGTGAT GAATCGGGTA 
AACCTGGGAT ATTTGCAAGA TGAGATGAAC GATCATCACC AGAACACCCT TTCCTATGTA 
CTCATCAACC CCCCGCCAGA CACAAGACTG GAACCCAACG ACATTGTGTA CCTCATCCGT 
TCCGACCCCC TGGCCCATGT GGCCAGCAGC TCCCAGAGTC GGAAAAGCAG CTGCAGCAAC 
AAGCTCTCAT CCTGTAATCC TGAGACCAGG GATGAGACCC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_030249649.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105509.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu87788
Clone ID Related Accession (Same CDS sequence)	XM_030249649.1
Accession Version	XM_030249649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC 
GGGAGGTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG 
TGCCTCATCG GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG 
CACATCCTGG CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA 
TATGAAGGGC CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGT GGCCATGGAC 
CTGCAGAACA CAGATTGCCG GCCCTCCCAG GGTGGCAGTG GCGGGGACGG CACAAAGCTG 
ACTCTGCCCA CCGAGAACGG CTCTGGTAGT CGACGCCCCA GCATCGCACC CGTTCTGGAG 
TTGGCAGACA GCTCAGCCCT GTTGCCCTGC GACCTGCTGA GTGACCAGTC AGAGGATGAG 
GTGACACCCT CGGACGACGA GGGGCTCTCT GTGGTTGAGT ACGTGAAGGG CTATCCCCCC 
AACTCACCCT ACATTGGCAG CTCCCCGACT TTATGCCACC TCCTGCCTGT GAAAGCCCCC 
TTCTGCTGCC TGCGGTTGGA CAAGGGCTGC AAACACAACA GCTACGAGGA TGCCAAGGCC 
TATGGGTTCA AGAACAAGCT GATTATTGTC TCTGCTGAGA CGGCAGGCAA CGGGCTCTAC 
AACTTCATCG TGCCTCTGCG CGCCTACTAC CGGTCCCGCC GGGAGCTCAA CCCTATCGTG 
TTGCTGCTTG ACAACAAGCC TGACCACCAC TTCCTGGAGG CCATCTGTTG CTTCCCCATG 
GTCTACTACA TGGAGGGATC CGTGGACAAC CTGGACAGCT TACTGCAGTG TGGCATCATC 
TATGCTGACA ACCTGGTGGT GGTGGACAAG GAGAGCACTA TGAGCGCTGA AGAGGACTAC 
ATGGCAGATG CCAAGACCAT CGTCAATGTG CAAACCATGT TCCGGCTTTT CCCCAGTCTC 
AGCATCACCA CGGAGCTCAC ACACCCTTCC AACATGCGGT TCATGCAGTT CCGTGCCAAG 
GACAGCTACT CTCTGGCTCT TTCCAAACTT GAAAAGCAAG AACGGGAGAA CGGCTCCAAC 
CTGGCCTTCA TGTTCCGCCT GCCATTTGCT GCTGGTCGAG TATTTAGTAT CAGCATGTTG 
GATACACTGC TCTACCAGTC CTTCGTGAAG GACTACATGA TCACCATCAC CAGGCTGCTC 
TTGGGCCTTG ATACTACACC AGGCTCCGGC TACCTCTGTG CAATGAAGGT AACCGAGGAC 
GACCTGTGGA TCCGCACTTA CGGCCGCCTC TTCCAGAAAC TCTGCTCCTC CAGCGCCGAG 
ATCCCCATCG GCATCTACAG GACCGAGTGC CATGTCTTCT CGGAGCCCCA TGACGTCAGA 
GCCCAGGGGG GCCCAGACAT AGCCCTCCTG CCTGCCCAGT CTCAGATCTC GGTGAACATG 
GAGGACTGCG AGGATACTCG GGAGGCCAAG GGACCCTGGG GCACACGAGC TGCATCTGGC 
AGTGGCAGCA CCCATGGCCG TCACGGGGGC AGTGCTGACC CAGTGGAGCA CCCACTACTA 
CGTCGCAAGA GCCTGCAGTG GGCCCGCAAG CTGAGTCGCA AGAGCACCAA GCAGGCAGGG 
AAGGCACCTG TGGCCACAGA CTGGATCACC CAGCAGCGGC TCAGCCTGTA CCGACGCTCA 
GAGCGCCAGG AGCTCTCAGA GCTGGTCAAG AACCGAATGA AGCACCTGGG ACTGCCCACC 
ACTGGCTATG AGGACGTAGC AAATTTAACA GCCAGTGATG TGATGAATCG GGTAAACCTG 
GGATATTTGC AAGATGAGAT GAACGATCAT CACCAGAACA CCCTTTCCTA TGTACTCATC 
AACCCCCCGC CAGACACAAG ACTGGAACCC AACGACATTG TGTACCTCAT CCGTTCCGAC 
CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG AGTCGGAAAA GCAGCTGCAG CAACAAGCTC 
TCATCCTGTA ATCCTGAGAC CAGGGATGAG ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105509.1
CDS	57..3758
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_030249649.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249649.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC 
GGGAGGTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG 
TGCCTCATCG GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG 
CACATCCTGG CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA 
TATGAAGGGC CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGT GGCCATGGAC 
CTGCAGAACA CAGATTGCCG GCCCTCCCAG GGTGGCAGTG GCGGGGACGG CACAAAGCTG 
ACTCTGCCCA CCGAGAACGG CTCTGGTAGT CGACGCCCCA GCATCGCACC CGTTCTGGAG 
TTGGCAGACA GCTCAGCCCT GTTGCCCTGC GACCTGCTGA GTGACCAGTC AGAGGATGAG 
GTGACACCCT CGGACGACGA GGGGCTCTCT GTGGTTGAGT ACGTGAAGGG CTATCCCCCC 
AACTCACCCT ACATTGGCAG CTCCCCGACT TTATGCCACC TCCTGCCTGT GAAAGCCCCC 
TTCTGCTGCC TGCGGTTGGA CAAGGGCTGC AAACACAACA GCTACGAGGA TGCCAAGGCC 
TATGGGTTCA AGAACAAGCT GATTATTGTC TCTGCTGAGA CGGCAGGCAA CGGGCTCTAC 
AACTTCATCG TGCCTCTGCG CGCCTACTAC CGGTCCCGCC GGGAGCTCAA CCCTATCGTG 
TTGCTGCTTG ACAACAAGCC TGACCACCAC TTCCTGGAGG CCATCTGTTG CTTCCCCATG 
GTCTACTACA TGGAGGGATC CGTGGACAAC CTGGACAGCT TACTGCAGTG TGGCATCATC 
TATGCTGACA ACCTGGTGGT GGTGGACAAG GAGAGCACTA TGAGCGCTGA AGAGGACTAC 
ATGGCAGATG CCAAGACCAT CGTCAATGTG CAAACCATGT TCCGGCTTTT CCCCAGTCTC 
AGCATCACCA CGGAGCTCAC ACACCCTTCC AACATGCGGT TCATGCAGTT CCGTGCCAAG 
GACAGCTACT CTCTGGCTCT TTCCAAACTT GAAAAGCAAG AACGGGAGAA CGGCTCCAAC 
CTGGCCTTCA TGTTCCGCCT GCCATTTGCT GCTGGTCGAG TATTTAGTAT CAGCATGTTG 
GATACACTGC TCTACCAGTC CTTCGTGAAG GACTACATGA TCACCATCAC CAGGCTGCTC 
TTGGGCCTTG ATACTACACC AGGCTCCGGC TACCTCTGTG CAATGAAGGT AACCGAGGAC 
GACCTGTGGA TCCGCACTTA CGGCCGCCTC TTCCAGAAAC TCTGCTCCTC CAGCGCCGAG 
ATCCCCATCG GCATCTACAG GACCGAGTGC CATGTCTTCT CGGAGCCCCA TGACGTCAGA 
GCCCAGGGGG GCCCAGACAT AGCCCTCCTG CCTGCCCAGT CTCAGATCTC GGTGAACATG 
GAGGACTGCG AGGATACTCG GGAGGCCAAG GGACCCTGGG GCACACGAGC TGCATCTGGC 
AGTGGCAGCA CCCATGGCCG TCACGGGGGC AGTGCTGACC CAGTGGAGCA CCCACTACTA 
CGTCGCAAGA GCCTGCAGTG GGCCCGCAAG CTGAGTCGCA AGAGCACCAA GCAGGCAGGG 
AAGGCACCTG TGGCCACAGA CTGGATCACC CAGCAGCGGC TCAGCCTGTA CCGACGCTCA 
GAGCGCCAGG AGCTCTCAGA GCTGGTCAAG AACCGAATGA AGCACCTGGG ACTGCCCACC 
ACTGGCTATG AGGACGTAGC AAATTTAACA GCCAGTGATG TGATGAATCG GGTAAACCTG 
GGATATTTGC AAGATGAGAT GAACGATCAT CACCAGAACA CCCTTTCCTA TGTACTCATC 
AACCCCCCGC CAGACACAAG ACTGGAACCC AACGACATTG TGTACCTCAT CCGTTCCGAC 
CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG AGTCGGAAAA GCAGCTGCAG CAACAAGCTC 
TCATCCTGTA ATCCTGAGAC CAGGGATGAG ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_030249663.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105523.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X15, mRNA.

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CloneID	OMu87789
Clone ID Related Accession (Same CDS sequence)	XM_030249663.1
Accession Version	XM_030249663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GGGAAGGACA GGAGTCTCCC GAGCAGTGGC 
AGCGCACGTA CGGGAGGTGC TCGGGCAACG AGGTGTACCA CATTCGCATG GGTGACAGTA 
AATTCTTCCG GGAGTATGAG GGCAAGAGCT TCACCTACGC AGCCTTCCAC GCGCACAAGA 
AGGACAGTGG CCATGGACCT GCAGAACACA GATTGCCGGC CCTCCCAGGG TGGCAGTGGC 
GGGGACGGCA CAAAGCTGAC TCTGCCCACC GAGAACGGCT CTGGTAGTCG ACGCCCCAGC 
ATCGCACCCG TTCTGGAGTT GGCAGACAGC TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT 
GACCAGTCAG AGGATGAGGT GACACCCTCG GACGACGAGG GGCTCTCTGT GGTTGAGTAC 
GTGAAGGGCT ATCCCCCCAA CTCACCCTAC ATTGGCAGCT CCCCGACTTT ATGCCACCTC 
CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG CGGTTGGACA AGGGCTGCAA ACACAACAGC 
TACGAGGATG CCAAGGCCTA TGGGTTCAAG AACAAGCTGA TTATTGTCTC TGCTGAGACG 
GCAGGCAACG GGCTCTACAA CTTCATCGTG CCTCTGCGCG CCTACTACCG GTCCCGCCGG 
GAGCTCAACC CTATCGTGTT GCTGCTTGAC AACAAGCCTG ACCACCACTT CCTGGAGGCC 
ATCTGTTGCT TCCCCATGGT CTACTACATG GAGGGATCCG TGGACAACCT GGACAGCTTA 
CTGCAGTGTG GCATCATCTA TGCTGACAAC CTGGTGGTGG TGGACAAGGA GAGCACTATG 
AGCGCTGAAG AGGACTACAT GGCAGATGCC AAGACCATCG TCAATGTGCA AACCATGTTC 
CGGCTTTTCC CCAGTCTCAG CATCACCACG GAGCTCACAC ACCCTTCCAA CATGCGGTTC 
ATGCAGTTCC GTGCCAAGGA CAGCTACTCT CTGGCTCTTT CCAAACTTGA AAAGCAAGAA 
CGGGAGAACG GCTCCAACCT GGCCTTCATG TTCCGCCTGC CATTTGCTGC TGGTCGAGTA 
TTTAGTATCA GCATGTTGGA TACACTGCTC TACCAGTCCT TCGTGAAGGA CTACATGATC 
ACCATCACCA GGCTGCTCTT GGGCCTTGAT ACTACACCAG GCTCCGGCTA CCTCTGTGCA 
ATGAAGGTAA CCGAGGACGA CCTGTGGATC CGCACTTACG GCCGCCTCTT CCAGAAACTC 
TGCTCCTCCA GCGCCGAGAT CCCCATCGGC ATCTACAGGA CCGAGTGCCA TGTCTTCTCG 
GAGCCCCATG ACGTCAGAGC CCAGGGGGGC CCAGACATAG CCCTCCTGCC TGCCCAGTCT 
CAGATCTCGG TGAACATGGA GGACTGCGAG GATACTCGGG AGGCCAAGGG ACCCTGGGGC 
ACACGAGCTG CATCTGGCAG TGGCAGCACC CATGGCCGTC ACGGGGGCAG TGCTGACCCA 
GTGGAGCACC CACTACTACG TCGCAAGAGC CTGCAGTGGG CCCGCAAGCT GAGTCGCAAG 
AGCACCAAGC AGGCAGGGAA GGCACCTGTG GCCACAGACT GGATCACCCA GCAGCGGCTC 
AGCCTGTACC GACGCTCAGA GCGCCAGGAG CTCTCAGAGC TGGTCAAGAA CCGAATGAAG 
CACCTGGGAC TGCCCACCAC TGGCTATGAG GACGTAGCAA ATTTAACAGC CAGTGATGTG 
ATGAATCGGG TAAACCTGGG ATATTTGCAA GATGAGATGA ACGATCATCA CCAGAACACC 
CTTTCCTATG TACTCATCAA CCCCCCGCCA GACACAAGAC TGGAACCCAA CGACATTGTG 
TACCTCATCC GTTCCGACCC CCTGGCCCAT GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC 
AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105523.1
CDS	59..3418
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAGKDRSLPSSGSARTGGARATRCTTFAWVTVNSSGSMRARASPTQPSTRTRRTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_030249663.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249663.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GGGAAGGACA GGAGTCTCCC GAGCAGTGGC 
AGCGCACGTA CGGGAGGTGC TCGGGCAACG AGGTGTACCA CATTCGCATG GGTGACAGTA 
AATTCTTCCG GGAGTATGAG GGCAAGAGCT TCACCTACGC AGCCTTCCAC GCGCACAAGA 
AGGACAGTGG CCATGGACCT GCAGAACACA GATTGCCGGC CCTCCCAGGG TGGCAGTGGC 
GGGGACGGCA CAAAGCTGAC TCTGCCCACC GAGAACGGCT CTGGTAGTCG ACGCCCCAGC 
ATCGCACCCG TTCTGGAGTT GGCAGACAGC TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT 
GACCAGTCAG AGGATGAGGT GACACCCTCG GACGACGAGG GGCTCTCTGT GGTTGAGTAC 
GTGAAGGGCT ATCCCCCCAA CTCACCCTAC ATTGGCAGCT CCCCGACTTT ATGCCACCTC 
CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG CGGTTGGACA AGGGCTGCAA ACACAACAGC 
TACGAGGATG CCAAGGCCTA TGGGTTCAAG AACAAGCTGA TTATTGTCTC TGCTGAGACG 
GCAGGCAACG GGCTCTACAA CTTCATCGTG CCTCTGCGCG CCTACTACCG GTCCCGCCGG 
GAGCTCAACC CTATCGTGTT GCTGCTTGAC AACAAGCCTG ACCACCACTT CCTGGAGGCC 
ATCTGTTGCT TCCCCATGGT CTACTACATG GAGGGATCCG TGGACAACCT GGACAGCTTA 
CTGCAGTGTG GCATCATCTA TGCTGACAAC CTGGTGGTGG TGGACAAGGA GAGCACTATG 
AGCGCTGAAG AGGACTACAT GGCAGATGCC AAGACCATCG TCAATGTGCA AACCATGTTC 
CGGCTTTTCC CCAGTCTCAG CATCACCACG GAGCTCACAC ACCCTTCCAA CATGCGGTTC 
ATGCAGTTCC GTGCCAAGGA CAGCTACTCT CTGGCTCTTT CCAAACTTGA AAAGCAAGAA 
CGGGAGAACG GCTCCAACCT GGCCTTCATG TTCCGCCTGC CATTTGCTGC TGGTCGAGTA 
TTTAGTATCA GCATGTTGGA TACACTGCTC TACCAGTCCT TCGTGAAGGA CTACATGATC 
ACCATCACCA GGCTGCTCTT GGGCCTTGAT ACTACACCAG GCTCCGGCTA CCTCTGTGCA 
ATGAAGGTAA CCGAGGACGA CCTGTGGATC CGCACTTACG GCCGCCTCTT CCAGAAACTC 
TGCTCCTCCA GCGCCGAGAT CCCCATCGGC ATCTACAGGA CCGAGTGCCA TGTCTTCTCG 
GAGCCCCATG ACGTCAGAGC CCAGGGGGGC CCAGACATAG CCCTCCTGCC TGCCCAGTCT 
CAGATCTCGG TGAACATGGA GGACTGCGAG GATACTCGGG AGGCCAAGGG ACCCTGGGGC 
ACACGAGCTG CATCTGGCAG TGGCAGCACC CATGGCCGTC ACGGGGGCAG TGCTGACCCA 
GTGGAGCACC CACTACTACG TCGCAAGAGC CTGCAGTGGG CCCGCAAGCT GAGTCGCAAG 
AGCACCAAGC AGGCAGGGAA GGCACCTGTG GCCACAGACT GGATCACCCA GCAGCGGCTC 
AGCCTGTACC GACGCTCAGA GCGCCAGGAG CTCTCAGAGC TGGTCAAGAA CCGAATGAAG 
CACCTGGGAC TGCCCACCAC TGGCTATGAG GACGTAGCAA ATTTAACAGC CAGTGATGTG 
ATGAATCGGG TAAACCTGGG ATATTTGCAA GATGAGATGA ACGATCATCA CCAGAACACC 
CTTTCCTATG TACTCATCAA CCCCCCGCCA GACACAAGAC TGGAACCCAA CGACATTGTG 
TACCTCATCC GTTCCGACCC CCTGGCCCAT GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC 
AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_030249674.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105534.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X17, mRNA.

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CloneID	OMu87790
Clone ID Related Accession (Same CDS sequence)	XM_030249674.1
Accession Version	XM_030249674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGACC TGGACACGGA GGTGCTGCCC TTGCCGCCAA GGTACCGATT CCGTGACCTG 
CTTTTAGGAG ACCAGCCATT CCCGAACGAC GATAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC 
GGGAGGTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG 
TGCCTCATCG GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG 
CACATCCTGG CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA 
TATGAAGGGC CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA 
AAGCTGACTC TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GATGAGGTGA CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT 
CCCCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC 
AAGGCCTATG GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT 
ATCGTGTTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGTTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTACT GCAGTGTGGC 
ATCATCTATG CTGACAACCT GGTGGTGGTG GACAAGGAGA GCACTATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATCGTC AATGTGCAAA CCATGTTCCG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACACAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGT 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGAGTATT TAGTATCAGC 
ATGTTGGATA CACTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTCTTGG GCCTTGATAC TACACCAGGC TCCGGCTACC TCTGTGCAAT GAAGGTAACC 
GAGGACGACC TGTGGATCCG CACTTACGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCCGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCGGA GCCCCATGAC 
GTCAGAGCCC AGGGGGGCCC AGACATAGCC CTCCTGCCTG CCCAGTCTCA GATCTCGGTG 
AACATGGAGG ACTGCGAGGA TACTCGGGAG GCCAAGGGAC CCTGGGGCAC ACGAGCTGCA 
TCTGGCAGTG GCAGCACCCA TGGCCGTCAC GGGGGCAGTG CTGACCCAGT GGAGCACCCA 
CTACTACGTC GCAAGAGCCT GCAGTGGGCC CGCAAGCTGA GTCGCAAGAG CACCAAGCAG 
GCAGGGAAGG CACCTGTGGC CACAGACTGG ATCACCCAGC AGCGGCTCAG CCTGTACCGA 
CGCTCAGAGC GCCAGGAGCT CTCAGAGCTG GTCAAGAACC GAATGAAGCA CCTGGGACTG 
CCCACCACTG GCTATGAGGA CGTAGCAAAT TTAACAGCCA GTGATGTGAT GAATCGGGTA 
AACCTGGGAT ATTTGCAAGA TGAGATGAAC GATCATCACC AGAACACCCT TTCCTATGTA 
CTCATCAACC CCCCGCCAGA CACAAGACTG GAACCCAACG ACATTGTGTA CCTCATCCGT 
TCCGACCCCC TGGCCCATGT GGCCAGCAGC TCCCAGAGTC GGAAAAGCAG CTGCAGCAAC 
AAGCTCTCAT CCTGTAATCC TGAGACCAGG GATGAGACCC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105534.1
CDS	140..3787
Translation	MNDLDTEVLPLPPRYRFRDLLLGDQPFPNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_030249674.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249674.1

ORF Insert Sequence:

ATGAACGACC TGGACACGGA GGTGCTGCCC TTGCCGCCAA GGTACCGATT CCGTGACCTG 
CTTTTAGGAG ACCAGCCATT CCCGAACGAC GATAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC 
GGGAGGTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG 
TGCCTCATCG GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG 
CACATCCTGG CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA 
TATGAAGGGC CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA 
AAGCTGACTC TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GATGAGGTGA CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT 
CCCCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC 
AAGGCCTATG GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT 
ATCGTGTTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGTTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTACT GCAGTGTGGC 
ATCATCTATG CTGACAACCT GGTGGTGGTG GACAAGGAGA GCACTATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATCGTC AATGTGCAAA CCATGTTCCG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACACAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGT 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGAGTATT TAGTATCAGC 
ATGTTGGATA CACTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTCTTGG GCCTTGATAC TACACCAGGC TCCGGCTACC TCTGTGCAAT GAAGGTAACC 
GAGGACGACC TGTGGATCCG CACTTACGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCCGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCGGA GCCCCATGAC 
GTCAGAGCCC AGGGGGGCCC AGACATAGCC CTCCTGCCTG CCCAGTCTCA GATCTCGGTG 
AACATGGAGG ACTGCGAGGA TACTCGGGAG GCCAAGGGAC CCTGGGGCAC ACGAGCTGCA 
TCTGGCAGTG GCAGCACCCA TGGCCGTCAC GGGGGCAGTG CTGACCCAGT GGAGCACCCA 
CTACTACGTC GCAAGAGCCT GCAGTGGGCC CGCAAGCTGA GTCGCAAGAG CACCAAGCAG 
GCAGGGAAGG CACCTGTGGC CACAGACTGG ATCACCCAGC AGCGGCTCAG CCTGTACCGA 
CGCTCAGAGC GCCAGGAGCT CTCAGAGCTG GTCAAGAACC GAATGAAGCA CCTGGGACTG 
CCCACCACTG GCTATGAGGA CGTAGCAAAT TTAACAGCCA GTGATGTGAT GAATCGGGTA 
AACCTGGGAT ATTTGCAAGA TGAGATGAAC GATCATCACC AGAACACCCT TTCCTATGTA 
CTCATCAACC CCCCGCCAGA CACAAGACTG GAACCCAACG ACATTGTGTA CCTCATCCGT 
TCCGACCCCC TGGCCCATGT GGCCAGCAGC TCCCAGAGTC GGAAAAGCAG CTGCAGCAAC 
AAGCTCTCAT CCTGTAATCC TGAGACCAGG GATGAGACCC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( NM_175462.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780671.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 1, mRNA.

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CloneID	OMu05439
Clone ID Related Accession (Same CDS sequence)	NM_175462.4
Accession Version	NM_175462.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC141190.1, FJ628361.1, AK048406.1, CD363556.1 and BI134322.1. On Oct 15, 2014 this sequence version replaced NM_175462.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGTTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTA CTGCAGTGTG GCATCATCTA TGCTGACAAC 
CTGGTGGTGG TGGACAAGGA GAGCACTATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATCG TCAATGTGCA AACCATGTTC CGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACAC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GTGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGAGTA TTTAGTATCA GCATGTTGGA TACACTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTCTT GGGCCTTGAT 
ACTACACCAG GCTCCGGCTA CCTCTGTGCA ATGAAGGTAA CCGAGGACGA CCTGTGGATC 
CGCACTTACG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCCGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCG GAGCCCCATG ACGTCAGAGC CCAGTCTCAG 
ATCTCGGTGA ACATGGAGGA CTGCGAGGAT ACTCGGGAGG CCAAGGGACC CTGGGGCACA 
CGAGCTGCAT CTGGCAGTGG CAGCACCCAT GGCCGTCACG GGGGCAGTGC TGACCCAGTG 
GAGCACCCAC TACTACGTCG CAAGAGCCTG CAGTGGGCCC GCAAGCTGAG TCGCAAGAGC 
ACCAAGCAGG CAGGGAAGGC ACCTGTGGCC ACAGACTGGA TCACCCAGCA GCGGCTCAGC 
CTGTACCGAC GCTCAGAGCG CCAGGAGCTC TCAGAGCTGG TCAAGAACCG AATGAAGCAC 
CTGGGACTGC CCACCACTGG CTATGAGGAC GTAGCAAATT TAACAGCCAG TGATGTGATG 
AATCGGGTAA ACCTGGGATA TTTGCAAGAT GAGATGAACG ATCATCACCA GAACACCCTT 
TCCTATGTAC TCATCAACCC CCCGCCAGAC ACAAGACTGG AACCCAACGA CATTGTGTAC 
CTCATCCGTT CCGACCCCCT GGCCCATGTG GCCAGCAGCT CCCAGAGTCG GAAAAGCAGC 
TGCAGCAACA AGCTCTCATC CTGTAATCCT GAGACCAGGG ATGAGACCCA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780671.2
CDS	99..3815
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	NM_175462.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175462.4

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGTTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTA CTGCAGTGTG GCATCATCTA TGCTGACAAC 
CTGGTGGTGG TGGACAAGGA GAGCACTATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATCG TCAATGTGCA AACCATGTTC CGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACAC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GTGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGAGTA TTTAGTATCA GCATGTTGGA TACACTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTCTT GGGCCTTGAT 
ACTACACCAG GCTCCGGCTA CCTCTGTGCA ATGAAGGTAA CCGAGGACGA CCTGTGGATC 
CGCACTTACG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCCGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCG GAGCCCCATG ACGTCAGAGC CCAGTCTCAG 
ATCTCGGTGA ACATGGAGGA CTGCGAGGAT ACTCGGGAGG CCAAGGGACC CTGGGGCACA 
CGAGCTGCAT CTGGCAGTGG CAGCACCCAT GGCCGTCACG GGGGCAGTGC TGACCCAGTG 
GAGCACCCAC TACTACGTCG CAAGAGCCTG CAGTGGGCCC GCAAGCTGAG TCGCAAGAGC 
ACCAAGCAGG CAGGGAAGGC ACCTGTGGCC ACAGACTGGA TCACCCAGCA GCGGCTCAGC 
CTGTACCGAC GCTCAGAGCG CCAGGAGCTC TCAGAGCTGG TCAAGAACCG AATGAAGCAC 
CTGGGACTGC CCACCACTGG CTATGAGGAC GTAGCAAATT TAACAGCCAG TGATGTGATG 
AATCGGGTAA ACCTGGGATA TTTGCAAGAT GAGATGAACG ATCATCACCA GAACACCCTT 
TCCTATGTAC TCATCAACCC CCCGCCAGAC ACAAGACTGG AACCCAACGA CATTGTGTAC 
CTCATCCGTT CCGACCCCCT GGCCCATGTG GCCAGCAGCT CCCAGAGTCG GAAAAGCAGC 
TGCAGCAACA AGCTCTCATC CTGTAATCCT GAGACCAGGG ATGAGACCCA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( NM_001145403.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001138875.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 2, mRNA.

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CloneID	OMu06861
Clone ID Related Accession (Same CDS sequence)	NM_001145403.2
Accession Version	NM_001145403.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY261633.1, CJ109324.1, FJ628361.1, AK048406.1, CD363556.1 and BI134322.1. On Oct 15, 2014 this sequence version replaced NM_001145403.1. Transcript Variant: This variant (2) contains an alternate 5' terminal exon, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164136 [ECO:0000348] ##Evidence-Data-END##

ATGAACGACC TGGACACGGA GGTGCTGCCC TTGCCGCCAA GGTACCGATT CCGTGACCTG 
CTTTTAGGAG ACCAGCCATT CCCGAACGAC GATAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGTTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTA CTGCAGTGTG GCATCATCTA TGCTGACAAC 
CTGGTGGTGG TGGACAAGGA GAGCACTATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATCG TCAATGTGCA AACCATGTTC CGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACAC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GTGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGAGTA TTTAGTATCA GCATGTTGGA TACACTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTCTT GGGCCTTGAT 
ACTACACCAG GCTCCGGCTA CCTCTGTGCA ATGAAGGTAA CCGAGGACGA CCTGTGGATC 
CGCACTTACG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCCGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCG GAGCCCCATG ACGTCAGAGC CCAGTCTCAG 
ATCTCGGTGA ACATGGAGGA CTGCGAGGAT ACTCGGGAGG CCAAGGGACC CTGGGGCACA 
CGAGCTGCAT CTGGCAGTGG CAGCACCCAT GGCCGTCACG GGGGCAGTGC TGACCCAGTG 
GAGCACCCAC TACTACGTCG CAAGAGCCTG CAGTGGGCCC GCAAGCTGAG TCGCAAGAGC 
ACCAAGCAGG CAGGGAAGGC ACCTGTGGCC ACAGACTGGA TCACCCAGCA GCGGCTCAGC 
CTGTACCGAC GCTCAGAGCG CCAGGAGCTC TCAGAGCTGG TCAAGAACCG AATGAAGCAC 
CTGGGACTGC CCACCACTGG CTATGAGGAC GTAGCAAATT TAACAGCCAG TGATGTGATG 
AATCGGGTAA ACCTGGGATA TTTGCAAGAT GAGATGAACG ATCATCACCA GAACACCCTT 
TCCTATGTAC TCATCAACCC CCCGCCAGAC ACAAGACTGG AACCCAACGA CATTGTGTAC 
CTCATCCGTT CCGACCCCCT GGCCCATGTG GCCAGCAGCT CCCAGAGTCG GAAAAGCAGC 
TGCAGCAACA AGCTCTCATC CTGTAATCCT GAGACCAGGG ATGAGACCCA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138875.1
CDS	174..3830
Translation	MNDLDTEVLPLPPRYRFRDLLLGDQPFPNDDRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	NM_001145403.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145403.2

ORF Insert Sequence:

ATGAACGACC TGGACACGGA GGTGCTGCCC TTGCCGCCAA GGTACCGATT CCGTGACCTG 
CTTTTAGGAG ACCAGCCATT CCCGAACGAC GATAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGTTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTA CTGCAGTGTG GCATCATCTA TGCTGACAAC 
CTGGTGGTGG TGGACAAGGA GAGCACTATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATCG TCAATGTGCA AACCATGTTC CGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACAC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GTGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGAGTA TTTAGTATCA GCATGTTGGA TACACTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTCTT GGGCCTTGAT 
ACTACACCAG GCTCCGGCTA CCTCTGTGCA ATGAAGGTAA CCGAGGACGA CCTGTGGATC 
CGCACTTACG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCCGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCG GAGCCCCATG ACGTCAGAGC CCAGTCTCAG 
ATCTCGGTGA ACATGGAGGA CTGCGAGGAT ACTCGGGAGG CCAAGGGACC CTGGGGCACA 
CGAGCTGCAT CTGGCAGTGG CAGCACCCAT GGCCGTCACG GGGGCAGTGC TGACCCAGTG 
GAGCACCCAC TACTACGTCG CAAGAGCCTG CAGTGGGCCC GCAAGCTGAG TCGCAAGAGC 
ACCAAGCAGG CAGGGAAGGC ACCTGTGGCC ACAGACTGGA TCACCCAGCA GCGGCTCAGC 
CTGTACCGAC GCTCAGAGCG CCAGGAGCTC TCAGAGCTGG TCAAGAACCG AATGAAGCAC 
CTGGGACTGC CCACCACTGG CTATGAGGAC GTAGCAAATT TAACAGCCAG TGATGTGATG 
AATCGGGTAA ACCTGGGATA TTTGCAAGAT GAGATGAACG ATCATCACCA GAACACCCTT 
TCCTATGTAC TCATCAACCC CCCGCCAGAC ACAAGACTGG AACCCAACGA CATTGTGTAC 
CTCATCCGTT CCGACCCCCT GGCCCATGTG GCCAGCAGCT CCCAGAGTCG GAAAAGCAGC 
TGCAGCAACA AGCTCTCATC CTGTAATCCT GAGACCAGGG ATGAGACCCA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( NM_001302351.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001289280.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu73345
Clone ID Related Accession (Same CDS sequence)	NM_001302351.1
Accession Version	NM_001302351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC141190.1, FJ628361.1, AK048406.1, CD363556.1 and BI134322.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC 
GGGAGGTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG 
TGCCTCATCG GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG 
CACATCCTGG CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA 
TATGAAGGGC CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA 
AAGCTGACTC TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GATGAGGTGA CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT 
CCCCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC 
AAGGCCTATG GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT 
ATCGTGTTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGTTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTACT GCAGTGTGGC 
ATCATCTATG CTGACAACCT GGTGGTGGTG GACAAGGAGA GCACTATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATCGTC AATGTGCAAA CCATGTTCCG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACACAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGT 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGAGTATT TAGTATCAGC 
ATGTTGGATA CACTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTCTTGG GCCTTGATAC TACACCAGGC TCCGGCTACC TCTGTGCAAT GAAGGTAACC 
GAGGACGACC TGTGGATCCG CACTTACGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCCGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCGGA GCCCCATGAC 
GTCAGAGCCC AGTCTCAGAT CTCGGTGAAC ATGGAGGACT GCGAGGATAC TCGGGAGGCC 
AAGGGACCCT GGGGCACACG AGCTGCATCT GGCAGTGGCA GCACCCATGG CCGTCACGGG 
GGCAGTGCTG ACCCAGTGGA GCACCCACTA CTACGTCGCA AGAGCCTGCA GTGGGCCCGC 
AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA GGGAAGGCAC CTGTGGCCAC AGACTGGATC 
ACCCAGCAGC GGCTCAGCCT GTACCGACGC TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGAA TGAAGCACCT GGGACTGCCC ACCACTGGCT ATGAGGACGT AGCAAATTTA 
ACAGCCAGTG ATGTGATGAA TCGGGTAAAC CTGGGATATT TGCAAGATGA GATGAACGAT 
CATCACCAGA ACACCCTTTC CTATGTACTC ATCAACCCCC CGCCAGACAC AAGACTGGAA 
CCCAACGACA TTGTGTACCT CATCCGTTCC GACCCCCTGG CCCATGTGGC CAGCAGCTCC 
CAGAGTCGGA AAAGCAGCTG CAGCAACAAG CTCTCATCCT GTAATCCTGA GACCAGGGAT 
GAGACCCAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289280.1
CDS	99..3773
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	NM_001302351.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302351.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC 
GGGAGGTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG 
TGCCTCATCG GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG 
CACATCCTGG CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA 
TATGAAGGGC CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA 
AAGCTGACTC TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GATGAGGTGA CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT 
CCCCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC 
AAGGCCTATG GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT 
ATCGTGTTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGTTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTACT GCAGTGTGGC 
ATCATCTATG CTGACAACCT GGTGGTGGTG GACAAGGAGA GCACTATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATCGTC AATGTGCAAA CCATGTTCCG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACACAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGT 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGAGTATT TAGTATCAGC 
ATGTTGGATA CACTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTCTTGG GCCTTGATAC TACACCAGGC TCCGGCTACC TCTGTGCAAT GAAGGTAACC 
GAGGACGACC TGTGGATCCG CACTTACGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCCGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCGGA GCCCCATGAC 
GTCAGAGCCC AGTCTCAGAT CTCGGTGAAC ATGGAGGACT GCGAGGATAC TCGGGAGGCC 
AAGGGACCCT GGGGCACACG AGCTGCATCT GGCAGTGGCA GCACCCATGG CCGTCACGGG 
GGCAGTGCTG ACCCAGTGGA GCACCCACTA CTACGTCGCA AGAGCCTGCA GTGGGCCCGC 
AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA GGGAAGGCAC CTGTGGCCAC AGACTGGATC 
ACCCAGCAGC GGCTCAGCCT GTACCGACGC TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGAA TGAAGCACCT GGGACTGCCC ACCACTGGCT ATGAGGACGT AGCAAATTTA 
ACAGCCAGTG ATGTGATGAA TCGGGTAAAC CTGGGATATT TGCAAGATGA GATGAACGAT 
CATCACCAGA ACACCCTTTC CTATGTACTC ATCAACCCCC CGCCAGACAC AAGACTGGAA 
CCCAACGACA TTGTGTACCT CATCCGTTCC GACCCCCTGG CCCATGTGGC CAGCAGCTCC 
CAGAGTCGGA AAAGCAGCTG CAGCAACAAG CTCTCATCCT GTAATCCTGA GACCAGGGAT 
GAGACCCAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497891.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497954.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X14, mRNA.

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CloneID	OMu29451
Clone ID Related Accession (Same CDS sequence)	XM_006497891.3 , XM_006497891.4
Accession Version	XM_006497891.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497891.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGACC TGGACACGGA GGTGCTGCCC TTGCCGCCAA GGTACCGATT CCGTGACCTG 
CTTTTAGGAG ACCAGCCATT CCCGAACGAC GATAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GCAGGACTAC TACGTGGTCA TCCTGTGTCC CTCTGAAATG 
GACGTCCAGG TGCGCAGGGT GCTGCAGATT CCCCTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCTG CCCTCAAGGA CCAGGATCTC ATGCGAGCCA AGATGGACAA CGGAGAGGCC 
TGCTTTATCC TCAGCAGCAG GAATGAGGTG GACCGCACAG CTGCGGATCA CCAGACCATC 
CTTCGAGCCT GGGCTGTGAA GGACTTTGCC CCCAACTGTC CCCTCTATGT CCAGATCCTC 
AAGCCCGAAA ACAAGTTTCA CGTCAAATTT GCTGACCACG TGGTATGCGA GGAAGAGTGC 
AAGTACGCCA TGCTGGCCCT GAACTGCATC TGCCCGGCCA CCTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGTGG CCAGGAAGGA CAGGAGTCTC CCGAGCAGTG GCAGCGCACG 
TACGGGAGGT GCTCGGGCAA CGAGGTGTAC CACATTCGCA TGGGTGACAG TAAATTCTTC 
CGGGAGTATG AGGGCAAGAG CTTCACCTAC GCAGCCTTCC ACGCGCACAA GAAATATGGG 
GTGTGCCTCA TCGGGCTGAA GCGTGAGGAG AACAAGAGTA TCCTGCTGAA CCCAGGACCA 
CGGCACATCC TGGCTGCCTC CGACACCTGC TTCTATATCA ATATTACCAA GGAGGAGAAC 
TCAGCTTTCA TCTTCAAACA GGAGGAGAAG CAGAAGAGAC GGGGCCTTGC AGGGCAGGCA 
CTATATGAAG GGCCCTCCCG GCTCCCAGTG CATAGCATCA TCGCCTCTAT GGGGACAGTG 
GCCATGGACC TGCAGAACAC AGATTGCCGG CCCTCCCAGG GTGGCAGTGG CGGGGACGGC 
ACAAAGCTGA CTCTGCCCAC CGAGAACGGC TCTGGTAGTC GACGCCCCAG CATCGCACCC 
GTTCTGGAGT TGGCAGACAG CTCAGCCCTG TTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGATGAGG TGACACCCTC GGACGACGAG GGGCTCTCTG TGGTTGAGTA CGTGAAGGGC 
TATCCCCCCA ACTCACCCTA CATTGGCAGC TCCCCGACTT TATGCCACCT CCTGCCTGTG 
AAAGCCCCCT TCTGCTGCCT GCGGTTGGAC AAGGGCTGCA AACACAACAG CTACGAGGAT 
GCCAAGGCCT ATGGGTTCAA GAACAAGCTG ATTATTGTCT CTGCTGAGAC GGCAGGCAAC 
GGGCTCTACA ACTTCATCGT GCCTCTGCGC GCCTACTACC GGTCCCGCCG GGAGCTCAAC 
CCTATCGTGT TGCTGCTTGA CAACAAGCCT GACCACCACT TCCTGGAGGC CATCTGTTGC 
TTCCCCATGG TCTACTACAT GGAGGGATCC GTGGACAACC TGGACAGCTT ACTGCAGTGT 
GGCATCATCT ATGCTGACAA CCTGGTGGTG GTGGACAAGG AGAGCACTAT GAGCGCTGAA 
GAGGACTACA TGGCAGATGC CAAGACCATC GTCAATGTGC AAACCATGTT CCGGCTTTTC 
CCCAGTCTCA GCATCACCAC GGAGCTCACA CACCCTTCCA ACATGCGGTT CATGCAGTTC 
CGTGCCAAGG ACAGCTACTC TCTGGCTCTT TCCAAACTTG AAAAGCAAGA ACGGGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCATTTGCTG CTGGTCGAGT ATTTAGTATC 
AGCATGTTGG ATACACTGCT CTACCAGTCC TTCGTGAAGG ACTACATGAT CACCATCACC 
AGGCTGCTCT TGGGCCTTGA TACTACACCA GGCTCCGGCT ACCTCTGTGC AATGAAGGTA 
ACCGAGGACG ACCTGTGGAT CCGCACTTAC GGCCGCCTCT TCCAGAAACT CTGCTCCTCC 
AGCGCCGAGA TCCCCATCGG CATCTACAGG ACCGAGTGCC ATGTCTTCTC GGAGCCCCAT 
GACGTCAGAG CCCAGGGGGG CCCAGACATA GCCCTCCTGC CTGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGACTGCGA GGATACTCGG GAGGCCAAGG GACCCTGGGG CACACGAGCT 
GCATCTGGCA GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC AGTGGAGCAC 
CCACTACTAC GTCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGTCGCAA GAGCACCAAG 
CAGGCAGGGA AGGCACCTGT GGCCACAGAC TGGATCACCC AGCAGCGGCT CAGCCTGTAC 
CGACGCTCAG AGCGCCAGGA GCTCTCAGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGA 
CTGCCCACCA CTGGCTATGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AACGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAACCCA ACGACATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA TGTGGCCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAGCTCT CATCCTGTAA TCCTGAGACC AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497954.1
CDS	153..3803
Translation	MNDLDTEVLPLPPRYRFRDLLLGDQPFPNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497891.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497891.3

ORF Insert Sequence:

ATGAACGACC TGGACACGGA GGTGCTGCCC TTGCCGCCAA GGTACCGATT CCGTGACCTG 
CTTTTAGGAG ACCAGCCATT CCCGAACGAC GATAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GCAGGACTAC TACGTGGTCA TCCTGTGTCC CTCTGAAATG 
GACGTCCAGG TGCGCAGGGT GCTGCAGATT CCCCTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCTG CCCTCAAGGA CCAGGATCTC ATGCGAGCCA AGATGGACAA CGGAGAGGCC 
TGCTTTATCC TCAGCAGCAG GAATGAGGTG GACCGCACAG CTGCGGATCA CCAGACCATC 
CTTCGAGCCT GGGCTGTGAA GGACTTTGCC CCCAACTGTC CCCTCTATGT CCAGATCCTC 
AAGCCCGAAA ACAAGTTTCA CGTCAAATTT GCTGACCACG TGGTATGCGA GGAAGAGTGC 
AAGTACGCCA TGCTGGCCCT GAACTGCATC TGCCCGGCCA CCTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGTGG CCAGGAAGGA CAGGAGTCTC CCGAGCAGTG GCAGCGCACG 
TACGGGAGGT GCTCGGGCAA CGAGGTGTAC CACATTCGCA TGGGTGACAG TAAATTCTTC 
CGGGAGTATG AGGGCAAGAG CTTCACCTAC GCAGCCTTCC ACGCGCACAA GAAATATGGG 
GTGTGCCTCA TCGGGCTGAA GCGTGAGGAG AACAAGAGTA TCCTGCTGAA CCCAGGACCA 
CGGCACATCC TGGCTGCCTC CGACACCTGC TTCTATATCA ATATTACCAA GGAGGAGAAC 
TCAGCTTTCA TCTTCAAACA GGAGGAGAAG CAGAAGAGAC GGGGCCTTGC AGGGCAGGCA 
CTATATGAAG GGCCCTCCCG GCTCCCAGTG CATAGCATCA TCGCCTCTAT GGGGACAGTG 
GCCATGGACC TGCAGAACAC AGATTGCCGG CCCTCCCAGG GTGGCAGTGG CGGGGACGGC 
ACAAAGCTGA CTCTGCCCAC CGAGAACGGC TCTGGTAGTC GACGCCCCAG CATCGCACCC 
GTTCTGGAGT TGGCAGACAG CTCAGCCCTG TTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGATGAGG TGACACCCTC GGACGACGAG GGGCTCTCTG TGGTTGAGTA CGTGAAGGGC 
TATCCCCCCA ACTCACCCTA CATTGGCAGC TCCCCGACTT TATGCCACCT CCTGCCTGTG 
AAAGCCCCCT TCTGCTGCCT GCGGTTGGAC AAGGGCTGCA AACACAACAG CTACGAGGAT 
GCCAAGGCCT ATGGGTTCAA GAACAAGCTG ATTATTGTCT CTGCTGAGAC GGCAGGCAAC 
GGGCTCTACA ACTTCATCGT GCCTCTGCGC GCCTACTACC GGTCCCGCCG GGAGCTCAAC 
CCTATCGTGT TGCTGCTTGA CAACAAGCCT GACCACCACT TCCTGGAGGC CATCTGTTGC 
TTCCCCATGG TCTACTACAT GGAGGGATCC GTGGACAACC TGGACAGCTT ACTGCAGTGT 
GGCATCATCT ATGCTGACAA CCTGGTGGTG GTGGACAAGG AGAGCACTAT GAGCGCTGAA 
GAGGACTACA TGGCAGATGC CAAGACCATC GTCAATGTGC AAACCATGTT CCGGCTTTTC 
CCCAGTCTCA GCATCACCAC GGAGCTCACA CACCCTTCCA ACATGCGGTT CATGCAGTTC 
CGTGCCAAGG ACAGCTACTC TCTGGCTCTT TCCAAACTTG AAAAGCAAGA ACGGGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCATTTGCTG CTGGTCGAGT ATTTAGTATC 
AGCATGTTGG ATACACTGCT CTACCAGTCC TTCGTGAAGG ACTACATGAT CACCATCACC 
AGGCTGCTCT TGGGCCTTGA TACTACACCA GGCTCCGGCT ACCTCTGTGC AATGAAGGTA 
ACCGAGGACG ACCTGTGGAT CCGCACTTAC GGCCGCCTCT TCCAGAAACT CTGCTCCTCC 
AGCGCCGAGA TCCCCATCGG CATCTACAGG ACCGAGTGCC ATGTCTTCTC GGAGCCCCAT 
GACGTCAGAG CCCAGGGGGG CCCAGACATA GCCCTCCTGC CTGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGACTGCGA GGATACTCGG GAGGCCAAGG GACCCTGGGG CACACGAGCT 
GCATCTGGCA GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC AGTGGAGCAC 
CCACTACTAC GTCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGTCGCAA GAGCACCAAG 
CAGGCAGGGA AGGCACCTGT GGCCACAGAC TGGATCACCC AGCAGCGGCT CAGCCTGTAC 
CGACGCTCAG AGCGCCAGGA GCTCTCAGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGA 
CTGCCCACCA CTGGCTATGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AACGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAACCCA ACGACATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA TGTGGCCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAGCTCT CATCCTGTAA TCCTGAGACC AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497892.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497955.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X19, mRNA.

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CloneID	OMu29452
Clone ID Related Accession (Same CDS sequence)	XM_006497892.4 , XM_006497892.3
Accession Version	XM_006497892.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497892.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGACC TGGACACGGA GGTGCTGCCC TTGCCGCCAA GGTACCGATT CCGTGACCTG 
CTTTTAGGAG ACCAGCCATT CCCGAACGAC GATAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC 
GGGAGGTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG 
TGCCTCATCG GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG 
CACATCCTGG CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA 
TATGAAGGGC CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA 
AAGCTGACTC TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GATGAGGTGA CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT 
CCCCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC 
AAGGCCTATG GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT 
ATCGTGTTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGTTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTACT GCAGTGTGGC 
ATCATCTATG CTGACAACCT GGTGGTGGTG GACAAGGAGA GCACTATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATCGTC AATGTGCAAA CCATGTTCCG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACACAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGT 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGAGTATT TAGTATCAGC 
ATGTTGGATA CACTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTCTTGG GCCTTGATAC TACACCAGGC TCCGGCTACC TCTGTGCAAT GAAGGTAACC 
GAGGACGACC TGTGGATCCG CACTTACGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCCGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCGGA GCCCCATGAC 
GTCAGAGCCC AGTCTCAGAT CTCGGTGAAC ATGGAGGACT GCGAGGATAC TCGGGAGGCC 
AAGGGACCCT GGGGCACACG AGCTGCATCT GGCAGTGGCA GCACCCATGG CCGTCACGGG 
GGCAGTGCTG ACCCAGTGGA GCACCCACTA CTACGTCGCA AGAGCCTGCA GTGGGCCCGC 
AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA GGGAAGGCAC CTGTGGCCAC AGACTGGATC 
ACCCAGCAGC GGCTCAGCCT GTACCGACGC TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGAA TGAAGCACCT GGGACTGCCC ACCACTGGCT ATGATGAGAT GAACGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAACCC 
AACGACATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAGCTC TCATCCTGTA ATCCTGAGAC CAGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497955.1
CDS	178..3729
Translation	MNDLDTEVLPLPPRYRFRDLLLGDQPFPNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497892.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497892.3

ORF Insert Sequence:

ATGAACGACC TGGACACGGA GGTGCTGCCC TTGCCGCCAA GGTACCGATT CCGTGACCTG 
CTTTTAGGAG ACCAGCCATT CCCGAACGAC GATAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC TGAAATGGAC 
GTCCAGGTGC GCAGGGTGCT GCAGATTCCC CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGATCTCATG CGAGCCAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAGGA CTTTGCCCCC AACTGTCCCC TCTATGTCCA GATCCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCACGTGG TATGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGTGGCCA GGAAGGACAG GAGTCTCCCG AGCAGTGGCA GCGCACGTAC 
GGGAGGTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA ATATGGGGTG 
TGCCTCATCG GGCTGAAGCG TGAGGAGAAC AAGAGTATCC TGCTGAACCC AGGACCACGG 
CACATCCTGG CTGCCTCCGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAACAGGA GGAGAAGCAG AAGAGACGGG GCCTTGCAGG GCAGGCACTA 
TATGAAGGGC CCTCCCGGCT CCCAGTGCAT AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGCGG GGACGGCACA 
AAGCTGACTC TGCCCACCGA GAACGGCTCT GGTAGTCGAC GCCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GATGAGGTGA CACCCTCGGA CGACGAGGGG CTCTCTGTGG TTGAGTACGT GAAGGGCTAT 
CCCCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GTTGGACAAG GGCTGCAAAC ACAACAGCTA CGAGGATGCC 
AAGGCCTATG GGTTCAAGAA CAAGCTGATT ATTGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGCGCC TACTACCGGT CCCGCCGGGA GCTCAACCCT 
ATCGTGTTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGTTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTACT GCAGTGTGGC 
ATCATCTATG CTGACAACCT GGTGGTGGTG GACAAGGAGA GCACTATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATCGTC AATGTGCAAA CCATGTTCCG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACACAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGT 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGAGTATT TAGTATCAGC 
ATGTTGGATA CACTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTCTTGG GCCTTGATAC TACACCAGGC TCCGGCTACC TCTGTGCAAT GAAGGTAACC 
GAGGACGACC TGTGGATCCG CACTTACGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCCGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCGGA GCCCCATGAC 
GTCAGAGCCC AGTCTCAGAT CTCGGTGAAC ATGGAGGACT GCGAGGATAC TCGGGAGGCC 
AAGGGACCCT GGGGCACACG AGCTGCATCT GGCAGTGGCA GCACCCATGG CCGTCACGGG 
GGCAGTGCTG ACCCAGTGGA GCACCCACTA CTACGTCGCA AGAGCCTGCA GTGGGCCCGC 
AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA GGGAAGGCAC CTGTGGCCAC AGACTGGATC 
ACCCAGCAGC GGCTCAGCCT GTACCGACGC TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGAA TGAAGCACCT GGGACTGCCC ACCACTGGCT ATGATGAGAT GAACGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAACCC 
AACGACATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAGCTC TCATCCTGTA ATCCTGAGAC CAGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497893.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497956.1 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OMu29453
Clone ID Related Accession (Same CDS sequence)	XM_006497893.4 , XM_006497893.3
Accession Version	XM_006497893.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497893.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAACC AGGTGCTCAT CCTGTTCTGC ACCCTGCTGT GCCTGGTCTT CACAGGGACC 
TGTGGGATCC AGCACCTGGA GCGAGCAGGT GGCAACTTGA ACCTGCTGAC CTCCTTCTAC 
TTCTGCATCG TGACTTTCTC AACTGTGGGC TTCGGTGATG TGACGCCCAA GATCTGGCCA 
TCCCAGCTCC TGGTGGTCAT CCTGATCTGT GTCACCCTTG TGGTGCTCCC ACTGCAATTT 
GAAGAGCTTG TCTACCTCTG GATGGAGCGT CAGAAGTCAG GGGGCAACTA TAGCCGCCAC 
CGAGCACGGA CGGAGAAGCA CGTAGTCCTG TGTGTGAGCT CCCTCAAGAT TGATCTCCTC 
ATGGACTTCC TGAATGAGTT CTATGCCCAT CCCCGGCTCC AGCAGGACTA CTACGTGGTC 
ATCCTGTGTC CCTCTGAAAT GGACGTCCAG GTGCGCAGGG TGCTGCAGAT TCCCCTGTGG 
TCCCAGCGGG TCATCTACCT CCAGGGCTCT GCCCTCAAGG ACCAGGATCT CATGCGAGCC 
AAGATGGACA ACGGAGAGGC CTGCTTTATC CTCAGCAGCA GGAATGAGGT GGACCGCACA 
GCTGCGGATC ACCAGACCAT CCTTCGAGCC TGGGCTGTGA AGGACTTTGC CCCCAACTGT 
CCCCTCTATG TCCAGATCCT CAAGCCCGAA AACAAGTTTC ACGTCAAATT TGCTGACCAC 
GTGGTATGCG AGGAAGAGTG CAAGTACGCC ATGCTGGCCC TGAACTGCAT CTGCCCGGCC 
ACCTCCACCC TCATCACCCT GCTGGTGCAC ACGTCCCGTG GCCAGGAAGG ACAGGAGTCT 
CCCGAGCAGT GGCAGCGCAC GTACGGGAGG TGCTCGGGCA ACGAGGTGTA CCACATTCGC 
ATGGGTGACA GTAAATTCTT CCGGGAGTAT GAGGGCAAGA GCTTCACCTA CGCAGCCTTC 
CACGCGCACA AGAAATATGG GGTGTGCCTC ATCGGGCTGA AGCGTGAGGA GAACAAGAGT 
ATCCTGCTGA ACCCAGGACC ACGGCACATC CTGGCTGCCT CCGACACCTG CTTCTATATC 
AATATTACCA AGGAGGAGAA CTCAGCTTTC ATCTTCAAAC AGGAGGAGAA GCAGAAGAGA 
CGGGGCCTTG CAGGGCAGGC ACTATATGAA GGGCCCTCCC GGCTCCCAGT GCATAGCATC 
ATCGCCTCTA TGGGGACAGT GGCCATGGAC CTGCAGAACA CAGATTGCCG GCCCTCCCAG 
GGTGGCAGTG GCGGGGACGG CACAAAGCTG ACTCTGCCCA CCGAGAACGG CTCTGGTAGT 
CGACGCCCCA GCATCGCACC CGTTCTGGAG TTGGCAGACA GCTCAGCCCT GTTGCCCTGC 
GACCTGCTGA GTGACCAGTC AGAGGATGAG GTGACACCCT CGGACGACGA GGGGCTCTCT 
GTGGTTGAGT ACGTGAAGGG CTATCCCCCC AACTCACCCT ACATTGGCAG CTCCCCGACT 
TTATGCCACC TCCTGCCTGT GAAAGCCCCC TTCTGCTGCC TGCGGTTGGA CAAGGGCTGC 
AAACACAACA GCTACGAGGA TGCCAAGGCC TATGGGTTCA AGAACAAGCT GATTATTGTC 
TCTGCTGAGA CGGCAGGCAA CGGGCTCTAC AACTTCATCG TGCCTCTGCG CGCCTACTAC 
CGGTCCCGCC GGGAGCTCAA CCCTATCGTG TTGCTGCTTG ACAACAAGCC TGACCACCAC 
TTCCTGGAGG CCATCTGTTG CTTCCCCATG GTCTACTACA TGGAGGGATC CGTGGACAAC 
CTGGACAGCT TACTGCAGTG TGGCATCATC TATGCTGACA ACCTGGTGGT GGTGGACAAG 
GAGAGCACTA TGAGCGCTGA AGAGGACTAC ATGGCAGATG CCAAGACCAT CGTCAATGTG 
CAAACCATGT TCCGGCTTTT CCCCAGTCTC AGCATCACCA CGGAGCTCAC ACACCCTTCC 
AACATGCGGT TCATGCAGTT CCGTGCCAAG GACAGCTACT CTCTGGCTCT TTCCAAACTT 
GAAAAGCAAG AACGGGAGAA CGGCTCCAAC CTGGCCTTCA TGTTCCGCCT GCCATTTGCT 
GCTGGTCGAG TATTTAGTAT CAGCATGTTG GATACACTGC TCTACCAGTC CTTCGTGAAG 
GACTACATGA TCACCATCAC CAGGCTGCTC TTGGGCCTTG ATACTACACC AGGCTCCGGC 
TACCTCTGTG CAATGAAGGT AACCGAGGAC GACCTGTGGA TCCGCACTTA CGGCCGCCTC 
TTCCAGAAAC TCTGCTCCTC CAGCGCCGAG ATCCCCATCG GCATCTACAG GACCGAGTGC 
CATGTCTTCT CGGAGCCCCA TGACGTCAGA GCCCAGGGGG GCCCAGACAT AGCCCTCCTG 
CCTGCCCAGT CTCAGATCTC GGTGAACATG GAGGACTGCG AGGATACTCG GGAGGCCAAG 
GGACCCTGGG GCACACGAGC TGCATCTGGC AGTGGCAGCA CCCATGGCCG TCACGGGGGC 
AGTGCTGACC CAGTGGAGCA CCCACTACTA CGTCGCAAGA GCCTGCAGTG GGCCCGCAAG 
CTGAGTCGCA AGAGCACCAA GCAGGCAGGG AAGGCACCTG TGGCCACAGA CTGGATCACC 
CAGCAGCGGC TCAGCCTGTA CCGACGCTCA GAGCGCCAGG AGCTCTCAGA GCTGGTCAAG 
AACCGAATGA AGCACCTGGG ACTGCCCACC ACTGGCTATG AGGACGTAGC AAATTTAACA 
GCCAGTGATG TGATGAATCG GGTAAACCTG GGATATTTGC AAGATGAGAT GAACGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAACCC 
AACGACATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAGCTC TCATCCTGTA ATCCTGAGAC CAGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497956.1
CDS	160..3171
Translation	MFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497893.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497893.3

ORF Insert Sequence:

ATGTTCAACC AGGTGCTCAT CCTGTTCTGC ACCCTGCTGT GCCTGGTCTT CACAGGGACC 
TGTGGGATCC AGCACCTGGA GCGAGCAGGT GGCAACTTGA ACCTGCTGAC CTCCTTCTAC 
TTCTGCATCG TGACTTTCTC AACTGTGGGC TTCGGTGATG TGACGCCCAA GATCTGGCCA 
TCCCAGCTCC TGGTGGTCAT CCTGATCTGT GTCACCCTTG TGGTGCTCCC ACTGCAATTT 
GAAGAGCTTG TCTACCTCTG GATGGAGCGT CAGAAGTCAG GGGGCAACTA TAGCCGCCAC 
CGAGCACGGA CGGAGAAGCA CGTAGTCCTG TGTGTGAGCT CCCTCAAGAT TGATCTCCTC 
ATGGACTTCC TGAATGAGTT CTATGCCCAT CCCCGGCTCC AGCAGGACTA CTACGTGGTC 
ATCCTGTGTC CCTCTGAAAT GGACGTCCAG GTGCGCAGGG TGCTGCAGAT TCCCCTGTGG 
TCCCAGCGGG TCATCTACCT CCAGGGCTCT GCCCTCAAGG ACCAGGATCT CATGCGAGCC 
AAGATGGACA ACGGAGAGGC CTGCTTTATC CTCAGCAGCA GGAATGAGGT GGACCGCACA 
GCTGCGGATC ACCAGACCAT CCTTCGAGCC TGGGCTGTGA AGGACTTTGC CCCCAACTGT 
CCCCTCTATG TCCAGATCCT CAAGCCCGAA AACAAGTTTC ACGTCAAATT TGCTGACCAC 
GTGGTATGCG AGGAAGAGTG CAAGTACGCC ATGCTGGCCC TGAACTGCAT CTGCCCGGCC 
ACCTCCACCC TCATCACCCT GCTGGTGCAC ACGTCCCGTG GCCAGGAAGG ACAGGAGTCT 
CCCGAGCAGT GGCAGCGCAC GTACGGGAGG TGCTCGGGCA ACGAGGTGTA CCACATTCGC 
ATGGGTGACA GTAAATTCTT CCGGGAGTAT GAGGGCAAGA GCTTCACCTA CGCAGCCTTC 
CACGCGCACA AGAAATATGG GGTGTGCCTC ATCGGGCTGA AGCGTGAGGA GAACAAGAGT 
ATCCTGCTGA ACCCAGGACC ACGGCACATC CTGGCTGCCT CCGACACCTG CTTCTATATC 
AATATTACCA AGGAGGAGAA CTCAGCTTTC ATCTTCAAAC AGGAGGAGAA GCAGAAGAGA 
CGGGGCCTTG CAGGGCAGGC ACTATATGAA GGGCCCTCCC GGCTCCCAGT GCATAGCATC 
ATCGCCTCTA TGGGGACAGT GGCCATGGAC CTGCAGAACA CAGATTGCCG GCCCTCCCAG 
GGTGGCAGTG GCGGGGACGG CACAAAGCTG ACTCTGCCCA CCGAGAACGG CTCTGGTAGT 
CGACGCCCCA GCATCGCACC CGTTCTGGAG TTGGCAGACA GCTCAGCCCT GTTGCCCTGC 
GACCTGCTGA GTGACCAGTC AGAGGATGAG GTGACACCCT CGGACGACGA GGGGCTCTCT 
GTGGTTGAGT ACGTGAAGGG CTATCCCCCC AACTCACCCT ACATTGGCAG CTCCCCGACT 
TTATGCCACC TCCTGCCTGT GAAAGCCCCC TTCTGCTGCC TGCGGTTGGA CAAGGGCTGC 
AAACACAACA GCTACGAGGA TGCCAAGGCC TATGGGTTCA AGAACAAGCT GATTATTGTC 
TCTGCTGAGA CGGCAGGCAA CGGGCTCTAC AACTTCATCG TGCCTCTGCG CGCCTACTAC 
CGGTCCCGCC GGGAGCTCAA CCCTATCGTG TTGCTGCTTG ACAACAAGCC TGACCACCAC 
TTCCTGGAGG CCATCTGTTG CTTCCCCATG GTCTACTACA TGGAGGGATC CGTGGACAAC 
CTGGACAGCT TACTGCAGTG TGGCATCATC TATGCTGACA ACCTGGTGGT GGTGGACAAG 
GAGAGCACTA TGAGCGCTGA AGAGGACTAC ATGGCAGATG CCAAGACCAT CGTCAATGTG 
CAAACCATGT TCCGGCTTTT CCCCAGTCTC AGCATCACCA CGGAGCTCAC ACACCCTTCC 
AACATGCGGT TCATGCAGTT CCGTGCCAAG GACAGCTACT CTCTGGCTCT TTCCAAACTT 
GAAAAGCAAG AACGGGAGAA CGGCTCCAAC CTGGCCTTCA TGTTCCGCCT GCCATTTGCT 
GCTGGTCGAG TATTTAGTAT CAGCATGTTG GATACACTGC TCTACCAGTC CTTCGTGAAG 
GACTACATGA TCACCATCAC CAGGCTGCTC TTGGGCCTTG ATACTACACC AGGCTCCGGC 
TACCTCTGTG CAATGAAGGT AACCGAGGAC GACCTGTGGA TCCGCACTTA CGGCCGCCTC 
TTCCAGAAAC TCTGCTCCTC CAGCGCCGAG ATCCCCATCG GCATCTACAG GACCGAGTGC 
CATGTCTTCT CGGAGCCCCA TGACGTCAGA GCCCAGGGGG GCCCAGACAT AGCCCTCCTG 
CCTGCCCAGT CTCAGATCTC GGTGAACATG GAGGACTGCG AGGATACTCG GGAGGCCAAG 
GGACCCTGGG GCACACGAGC TGCATCTGGC AGTGGCAGCA CCCATGGCCG TCACGGGGGC 
AGTGCTGACC CAGTGGAGCA CCCACTACTA CGTCGCAAGA GCCTGCAGTG GGCCCGCAAG 
CTGAGTCGCA AGAGCACCAA GCAGGCAGGG AAGGCACCTG TGGCCACAGA CTGGATCACC 
CAGCAGCGGC TCAGCCTGTA CCGACGCTCA GAGCGCCAGG AGCTCTCAGA GCTGGTCAAG 
AACCGAATGA AGCACCTGGG ACTGCCCACC ACTGGCTATG AGGACGTAGC AAATTTAACA 
GCCAGTGATG TGATGAATCG GGTAAACCTG GGATATTTGC AAGATGAGAT GAACGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAACCC 
AACGACATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ATGTGGCCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAGCTC TCATCCTGTA ATCCTGAGAC CAGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497877.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497940.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X1, mRNA.

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CloneID	OMu73346
Clone ID Related Accession (Same CDS sequence)	XM_006497877.3 , XM_006497877.4
Accession Version	XM_006497877.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497877.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGT CCTTCGTGAA GGACTACATG ATCACCATCA CCAGGCTGCT CTTGGGCCTT 
GATACTACAC CAGGCTCCGG CTACCTCTGT GCAATGAAGG TAACCGAGGA CGACCTGTGG 
ATCCGCACTT ACGGCCGCCT CTTCCAGAAA CTCTGCTCCT CCAGCGCCGA GATCCCCATC 
GGCATCTACA GGACCGAGTG CCATGTCTTC TCGGAGCCCC ATGACGTCAG AGCCCAGGGG 
GGCCCAGACA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGACTGC 
GAGGATACTC GGGAGGCCAA GGGACCCTGG GGCACACGAG CTGCATCTGG CAGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCAGTGGAGC ACCCACTACT ACGTCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGTCGC AAGAGCACCA AGCAGGCAGG GAAGGCACCT 
GTGGCCACAG ACTGGATCAC CCAGCAGCGG CTCAGCCTGT ACCGACGCTC AGAGCGCCAG 
GAGCTCTCAG AGCTGGTCAA GAACCGAATG AAGCACCTGG GACTGCCCAC CACTGGCTAT 
GAGGACGTAG CAAATTTAAC AGCCAGTGAT GTGATGAATC GGGTAAACCT GGGATATTTG 
CAAGATGAGA TGAACGATCA TCACCAGAAC ACCCTTTCCT ATGTACTCAT CAACCCCCCG 
CCAGACACAA GACTGGAACC CAACGACATT GTGTACCTCA TCCGTTCCGA CCCCCTGGCC 
CATGTGGCCA GCAGCTCCCA GAGTCGGAAA AGCAGCTGCA GCAACAAGCT CTCATCCTGT 
AATCCTGAGA CCAGGGATGA GACCCAGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497940.2
CDS	58..3810
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497877.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497877.3

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGT CCTTCGTGAA GGACTACATG ATCACCATCA CCAGGCTGCT CTTGGGCCTT 
GATACTACAC CAGGCTCCGG CTACCTCTGT GCAATGAAGG TAACCGAGGA CGACCTGTGG 
ATCCGCACTT ACGGCCGCCT CTTCCAGAAA CTCTGCTCCT CCAGCGCCGA GATCCCCATC 
GGCATCTACA GGACCGAGTG CCATGTCTTC TCGGAGCCCC ATGACGTCAG AGCCCAGGGG 
GGCCCAGACA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGACTGC 
GAGGATACTC GGGAGGCCAA GGGACCCTGG GGCACACGAG CTGCATCTGG CAGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCAGTGGAGC ACCCACTACT ACGTCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGTCGC AAGAGCACCA AGCAGGCAGG GAAGGCACCT 
GTGGCCACAG ACTGGATCAC CCAGCAGCGG CTCAGCCTGT ACCGACGCTC AGAGCGCCAG 
GAGCTCTCAG AGCTGGTCAA GAACCGAATG AAGCACCTGG GACTGCCCAC CACTGGCTAT 
GAGGACGTAG CAAATTTAAC AGCCAGTGAT GTGATGAATC GGGTAAACCT GGGATATTTG 
CAAGATGAGA TGAACGATCA TCACCAGAAC ACCCTTTCCT ATGTACTCAT CAACCCCCCG 
CCAGACACAA GACTGGAACC CAACGACATT GTGTACCTCA TCCGTTCCGA CCCCCTGGCC 
CATGTGGCCA GCAGCTCCCA GAGTCGGAAA AGCAGCTGCA GCAACAAGCT CTCATCCTGT 
AATCCTGAGA CCAGGGATGA GACCCAGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497878.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497941.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu73347
Clone ID Related Accession (Same CDS sequence)	XM_006497878.3 , XM_006497878.4
Accession Version	XM_006497878.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497878.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGTTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTA CTGCAGTGTG GCATCATCTA TGCTGACAAC 
CTGGTGGTGG TGGACAAGGA GAGCACTATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATCG TCAATGTGCA AACCATGTTC CGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACAC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GTGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGAGTA TTTAGTATCA GCATGTTGGA TACACTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTCTT GGGCCTTGAT 
ACTACACCAG GCTCCGGCTA CCTCTGTGCA ATGAAGGTAA CCGAGGACGA CCTGTGGATC 
CGCACTTACG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCCGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCG GAGCCCCATG ACGTCAGAGC CCAGGGGGGC 
CCAGACATAG CCCTCCTGCC TGCCCAGTCT CAGATCTCGG TGAACATGGA GGACTGCGAG 
GATACTCGGG AGGCCAAGGG ACCCTGGGGC ACACGAGCTG CATCTGGCAG TGGCAGCACC 
CATGGCCGTC ACGGGGGCAG TGCTGACCCA GTGGAGCACC CACTACTACG TCGCAAGAGC 
CTGCAGTGGG CCCGCAAGCT GAGTCGCAAG AGCACCAAGC AGGCAGGGAA GGCACCTGTG 
GCCACAGACT GGATCACCCA GCAGCGGCTC AGCCTGTACC GACGCTCAGA GCGCCAGGAG 
CTCTCAGAGC TGGTCAAGAA CCGAATGAAG CACCTGGGAC TGCCCACCAC TGGCTATGAG 
GACGTAGCAA ATTTAACAGC CAGTGATGTG ATGAATCGGG TAAACCTGGG ATATTTGCAA 
GATGAGATGA ACGATCATCA CCAGAACACC CTTTCCTATG TACTCATCAA CCCCCCGCCA 
GACACAAGAC TGGAACCCAA CGACATTGTG TACCTCATCC GTTCCGACCC CCTGGCCCAT 
GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT 
CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497941.2
CDS	57..3806
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497878.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497878.3

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGC GGGGACGGCA CAAAGCTGAC TCTGCCCACC 
GAGAACGGCT CTGGTAGTCG ACGCCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGATGAGGT GACACCCTCG 
GACGACGAGG GGCTCTCTGT GGTTGAGTAC GTGAAGGGCT ATCCCCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGTTGGACA AGGGCTGCAA ACACAACAGC TACGAGGATG CCAAGGCCTA TGGGTTCAAG 
AACAAGCTGA TTATTGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGCG CCTACTACCG GTCCCGCCGG GAGCTCAACC CTATCGTGTT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGTTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTA CTGCAGTGTG GCATCATCTA TGCTGACAAC 
CTGGTGGTGG TGGACAAGGA GAGCACTATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATCG TCAATGTGCA AACCATGTTC CGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACAC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GTGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGAGTA TTTAGTATCA GCATGTTGGA TACACTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTCTT GGGCCTTGAT 
ACTACACCAG GCTCCGGCTA CCTCTGTGCA ATGAAGGTAA CCGAGGACGA CCTGTGGATC 
CGCACTTACG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCCGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCG GAGCCCCATG ACGTCAGAGC CCAGGGGGGC 
CCAGACATAG CCCTCCTGCC TGCCCAGTCT CAGATCTCGG TGAACATGGA GGACTGCGAG 
GATACTCGGG AGGCCAAGGG ACCCTGGGGC ACACGAGCTG CATCTGGCAG TGGCAGCACC 
CATGGCCGTC ACGGGGGCAG TGCTGACCCA GTGGAGCACC CACTACTACG TCGCAAGAGC 
CTGCAGTGGG CCCGCAAGCT GAGTCGCAAG AGCACCAAGC AGGCAGGGAA GGCACCTGTG 
GCCACAGACT GGATCACCCA GCAGCGGCTC AGCCTGTACC GACGCTCAGA GCGCCAGGAG 
CTCTCAGAGC TGGTCAAGAA CCGAATGAAG CACCTGGGAC TGCCCACCAC TGGCTATGAG 
GACGTAGCAA ATTTAACAGC CAGTGATGTG ATGAATCGGG TAAACCTGGG ATATTTGCAA 
GATGAGATGA ACGATCATCA CCAGAACACC CTTTCCTATG TACTCATCAA CCCCCCGCCA 
GACACAAGAC TGGAACCCAA CGACATTGTG TACCTCATCC GTTCCGACCC CCTGGCCCAT 
GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT 
CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497879.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497942.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu73348
Clone ID Related Accession (Same CDS sequence)	XM_006497879.4 , XM_006497879.3
Accession Version	XM_006497879.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497879.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGTGGCCA TGGACCTGCA GAACACAGAT 
TGCCGGCCCT CCCAGGGTGG CAGTGGCGGG GACGGCACAA AGCTGACTCT GCCCACCGAG 
AACGGCTCTG GTAGTCGACG CCCCAGCATC GCACCCGTTC TGGAGTTGGC AGACAGCTCA 
GCCCTGTTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ATGAGGTGAC ACCCTCGGAC 
GACGAGGGGC TCTCTGTGGT TGAGTACGTG AAGGGCTATC CCCCCAACTC ACCCTACATT 
GGCAGCTCCC CGACTTTATG CCACCTCCTG CCTGTGAAAG CCCCCTTCTG CTGCCTGCGG 
TTGGACAAGG GCTGCAAACA CAACAGCTAC GAGGATGCCA AGGCCTATGG GTTCAAGAAC 
AAGCTGATTA TTGTCTCTGC TGAGACGGCA GGCAACGGGC TCTACAACTT CATCGTGCCT 
CTGCGCGCCT ACTACCGGTC CCGCCGGGAG CTCAACCCTA TCGTGTTGCT GCTTGACAAC 
AAGCCTGACC ACCACTTCCT GGAGGCCATC TGTTGCTTCC CCATGGTCTA CTACATGGAG 
GGATCCGTGG ACAACCTGGA CAGCTTACTG CAGTGTGGCA TCATCTATGC TGACAACCTG 
GTGGTGGTGG ACAAGGAGAG CACTATGAGC GCTGAAGAGG ACTACATGGC AGATGCCAAG 
ACCATCGTCA ATGTGCAAAC CATGTTCCGG CTTTTCCCCA GTCTCAGCAT CACCACGGAG 
CTCACACACC CTTCCAACAT GCGGTTCATG CAGTTCCGTG CCAAGGACAG CTACTCTCTG 
GCTCTTTCCA AACTTGAAAA GCAAGAACGG GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCAT TTGCTGCTGG TCGAGTATTT AGTATCAGCA TGTTGGATAC ACTGCTCTAC 
CAGTCCTTCG TGAAGGACTA CATGATCACC ATCACCAGGC TGCTCTTGGG CCTTGATACT 
ACACCAGGCT CCGGCTACCT CTGTGCAATG AAGGTAACCG AGGACGACCT GTGGATCCGC 
ACTTACGGCC GCCTCTTCCA GAAACTCTGC TCCTCCAGCG CCGAGATCCC CATCGGCATC 
TACAGGACCG AGTGCCATGT CTTCTCGGAG CCCCATGACG TCAGAGCCCA GGGGGGCCCA 
GACATAGCCC TCCTGCCTGC CCAGTCTCAG ATCTCGGTGA ACATGGAGGA CTGCGAGGAT 
ACTCGGGAGG CCAAGGGACC CTGGGGCACA CGAGCTGCAT CTGGCAGTGG CAGCACCCAT 
GGCCGTCACG GGGGCAGTGC TGACCCAGTG GAGCACCCAC TACTACGTCG CAAGAGCCTG 
CAGTGGGCCC GCAAGCTGAG TCGCAAGAGC ACCAAGCAGG CAGGGAAGGC ACCTGTGGCC 
ACAGACTGGA TCACCCAGCA GCGGCTCAGC CTGTACCGAC GCTCAGAGCG CCAGGAGCTC 
TCAGAGCTGG TCAAGAACCG AATGAAGCAC CTGGGACTGC CCACCACTGG CTATGAGGAC 
GTAGCAAATT TAACAGCCAG TGATGTGATG AATCGGGTAA ACCTGGGATA TTTGCAAGAT 
GAGATGAACG ATCATCACCA GAACACCCTT TCCTATGTAC TCATCAACCC CCCGCCAGAC 
ACAAGACTGG AACCCAACGA CATTGTGTAC CTCATCCGTT CCGACCCCCT GGCCCATGTG 
GCCAGCAGCT CCCAGAGTCG GAAAAGCAGC TGCAGCAACA AGCTCTCATC CTGTAATCCT 
GAGACCAGGG ATGAGACCCA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497942.2
CDS	59..3805
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497879.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497879.3

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGTGGCCA TGGACCTGCA GAACACAGAT 
TGCCGGCCCT CCCAGGGTGG CAGTGGCGGG GACGGCACAA AGCTGACTCT GCCCACCGAG 
AACGGCTCTG GTAGTCGACG CCCCAGCATC GCACCCGTTC TGGAGTTGGC AGACAGCTCA 
GCCCTGTTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ATGAGGTGAC ACCCTCGGAC 
GACGAGGGGC TCTCTGTGGT TGAGTACGTG AAGGGCTATC CCCCCAACTC ACCCTACATT 
GGCAGCTCCC CGACTTTATG CCACCTCCTG CCTGTGAAAG CCCCCTTCTG CTGCCTGCGG 
TTGGACAAGG GCTGCAAACA CAACAGCTAC GAGGATGCCA AGGCCTATGG GTTCAAGAAC 
AAGCTGATTA TTGTCTCTGC TGAGACGGCA GGCAACGGGC TCTACAACTT CATCGTGCCT 
CTGCGCGCCT ACTACCGGTC CCGCCGGGAG CTCAACCCTA TCGTGTTGCT GCTTGACAAC 
AAGCCTGACC ACCACTTCCT GGAGGCCATC TGTTGCTTCC CCATGGTCTA CTACATGGAG 
GGATCCGTGG ACAACCTGGA CAGCTTACTG CAGTGTGGCA TCATCTATGC TGACAACCTG 
GTGGTGGTGG ACAAGGAGAG CACTATGAGC GCTGAAGAGG ACTACATGGC AGATGCCAAG 
ACCATCGTCA ATGTGCAAAC CATGTTCCGG CTTTTCCCCA GTCTCAGCAT CACCACGGAG 
CTCACACACC CTTCCAACAT GCGGTTCATG CAGTTCCGTG CCAAGGACAG CTACTCTCTG 
GCTCTTTCCA AACTTGAAAA GCAAGAACGG GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCAT TTGCTGCTGG TCGAGTATTT AGTATCAGCA TGTTGGATAC ACTGCTCTAC 
CAGTCCTTCG TGAAGGACTA CATGATCACC ATCACCAGGC TGCTCTTGGG CCTTGATACT 
ACACCAGGCT CCGGCTACCT CTGTGCAATG AAGGTAACCG AGGACGACCT GTGGATCCGC 
ACTTACGGCC GCCTCTTCCA GAAACTCTGC TCCTCCAGCG CCGAGATCCC CATCGGCATC 
TACAGGACCG AGTGCCATGT CTTCTCGGAG CCCCATGACG TCAGAGCCCA GGGGGGCCCA 
GACATAGCCC TCCTGCCTGC CCAGTCTCAG ATCTCGGTGA ACATGGAGGA CTGCGAGGAT 
ACTCGGGAGG CCAAGGGACC CTGGGGCACA CGAGCTGCAT CTGGCAGTGG CAGCACCCAT 
GGCCGTCACG GGGGCAGTGC TGACCCAGTG GAGCACCCAC TACTACGTCG CAAGAGCCTG 
CAGTGGGCCC GCAAGCTGAG TCGCAAGAGC ACCAAGCAGG CAGGGAAGGC ACCTGTGGCC 
ACAGACTGGA TCACCCAGCA GCGGCTCAGC CTGTACCGAC GCTCAGAGCG CCAGGAGCTC 
TCAGAGCTGG TCAAGAACCG AATGAAGCAC CTGGGACTGC CCACCACTGG CTATGAGGAC 
GTAGCAAATT TAACAGCCAG TGATGTGATG AATCGGGTAA ACCTGGGATA TTTGCAAGAT 
GAGATGAACG ATCATCACCA GAACACCCTT TCCTATGTAC TCATCAACCC CCCGCCAGAC 
ACAAGACTGG AACCCAACGA CATTGTGTAC CTCATCCGTT CCGACCCCCT GGCCCATGTG 
GCCAGCAGCT CCCAGAGTCG GAAAAGCAGC TGCAGCAACA AGCTCTCATC CTGTAATCCT 
GAGACCAGGG ATGAGACCCA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497880.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497943.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X4, mRNA.

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CloneID	OMu73349
Clone ID Related Accession (Same CDS sequence)	XM_006497880.4 , XM_006497880.3
Accession Version	XM_006497880.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGT CCTTCGTGAA GGACTACATG ATCACCATCA CCAGGCTGCT CTTGGGCCTT 
GATACTACAC CAGGCTCCGG CTACCTCTGT GCAATGAAGG TAACCGAGGA CGACCTGTGG 
ATCCGCACTT ACGGCCGCCT CTTCCAGAAA CTCTGCTCCT CCAGCGCCGA GATCCCCATC 
GGCATCTACA GGACCGAGTG CCATGTCTTC TCGGAGCCCC ATGACGTCAG AGCCCAGTCT 
CAGATCTCGG TGAACATGGA GGACTGCGAG GATACTCGGG AGGCCAAGGG ACCCTGGGGC 
ACACGAGCTG CATCTGGCAG TGGCAGCACC CATGGCCGTC ACGGGGGCAG TGCTGACCCA 
GTGGAGCACC CACTACTACG TCGCAAGAGC CTGCAGTGGG CCCGCAAGCT GAGTCGCAAG 
AGCACCAAGC AGGCAGGGAA GGCACCTGTG GCCACAGACT GGATCACCCA GCAGCGGCTC 
AGCCTGTACC GACGCTCAGA GCGCCAGGAG CTCTCAGAGC TGGTCAAGAA CCGAATGAAG 
CACCTGGGAC TGCCCACCAC TGGCTATGAG GACGTAGCAA ATTTAACAGC CAGTGATGTG 
ATGAATCGGG TAAACCTGGG ATATTTGCAA GATGAGATGA ACGATCATCA CCAGAACACC 
CTTTCCTATG TACTCATCAA CCCCCCGCCA GACACAAGAC TGGAACCCAA CGACATTGTG 
TACCTCATCC GTTCCGACCC CCTGGCCCAT GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC 
AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497943.2
CDS	57..3776
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497880.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497880.3

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGT CCTTCGTGAA GGACTACATG ATCACCATCA CCAGGCTGCT CTTGGGCCTT 
GATACTACAC CAGGCTCCGG CTACCTCTGT GCAATGAAGG TAACCGAGGA CGACCTGTGG 
ATCCGCACTT ACGGCCGCCT CTTCCAGAAA CTCTGCTCCT CCAGCGCCGA GATCCCCATC 
GGCATCTACA GGACCGAGTG CCATGTCTTC TCGGAGCCCC ATGACGTCAG AGCCCAGTCT 
CAGATCTCGG TGAACATGGA GGACTGCGAG GATACTCGGG AGGCCAAGGG ACCCTGGGGC 
ACACGAGCTG CATCTGGCAG TGGCAGCACC CATGGCCGTC ACGGGGGCAG TGCTGACCCA 
GTGGAGCACC CACTACTACG TCGCAAGAGC CTGCAGTGGG CCCGCAAGCT GAGTCGCAAG 
AGCACCAAGC AGGCAGGGAA GGCACCTGTG GCCACAGACT GGATCACCCA GCAGCGGCTC 
AGCCTGTACC GACGCTCAGA GCGCCAGGAG CTCTCAGAGC TGGTCAAGAA CCGAATGAAG 
CACCTGGGAC TGCCCACCAC TGGCTATGAG GACGTAGCAA ATTTAACAGC CAGTGATGTG 
ATGAATCGGG TAAACCTGGG ATATTTGCAA GATGAGATGA ACGATCATCA CCAGAACACC 
CTTTCCTATG TACTCATCAA CCCCCCGCCA GACACAAGAC TGGAACCCAA CGACATTGTG 
TACCTCATCC GTTCCGACCC CCTGGCCCAT GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC 
AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497881.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497944.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu73350
Clone ID Related Accession (Same CDS sequence)	XM_006497881.4 , XM_006497881.3
Accession Version	XM_006497881.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497881.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GTGGCCATGG ACCTGCAGAA CACAGATTGC 
CGGCCCTCCC AGGGTGGCAG TGGCGGGGAC GGCACAAAGC TGACTCTGCC CACCGAGAAC 
GGCTCTGGTA GTCGACGCCC CAGCATCGCA CCCGTTCTGG AGTTGGCAGA CAGCTCAGCC 
CTGTTGCCCT GCGACCTGCT GAGTGACCAG TCAGAGGATG AGGTGACACC CTCGGACGAC 
GAGGGGCTCT CTGTGGTTGA GTACGTGAAG GGCTATCCCC CCAACTCACC CTACATTGGC 
AGCTCCCCGA CTTTATGCCA CCTCCTGCCT GTGAAAGCCC CCTTCTGCTG CCTGCGGTTG 
GACAAGGGCT GCAAACACAA CAGCTACGAG GATGCCAAGG CCTATGGGTT CAAGAACAAG 
CTGATTATTG TCTCTGCTGA GACGGCAGGC AACGGGCTCT ACAACTTCAT CGTGCCTCTG 
CGCGCCTACT ACCGGTCCCG CCGGGAGCTC AACCCTATCG TGTTGCTGCT TGACAACAAG 
CCTGACCACC ACTTCCTGGA GGCCATCTGT TGCTTCCCCA TGGTCTACTA CATGGAGGGA 
TCCGTGGACA ACCTGGACAG CTTACTGCAG TGTGGCATCA TCTATGCTGA CAACCTGGTG 
GTGGTGGACA AGGAGAGCAC TATGAGCGCT GAAGAGGACT ACATGGCAGA TGCCAAGACC 
ATCGTCAATG TGCAAACCAT GTTCCGGCTT TTCCCCAGTC TCAGCATCAC CACGGAGCTC 
ACACACCCTT CCAACATGCG GTTCATGCAG TTCCGTGCCA AGGACAGCTA CTCTCTGGCT 
CTTTCCAAAC TTGAAAAGCA AGAACGGGAG AACGGCTCCA ACCTGGCCTT CATGTTCCGC 
CTGCCATTTG CTGCTGGTCG AGTATTTAGT ATCAGCATGT TGGATACACT GCTCTACCAG 
TCCTTCGTGA AGGACTACAT GATCACCATC ACCAGGCTGC TCTTGGGCCT TGATACTACA 
CCAGGCTCCG GCTACCTCTG TGCAATGAAG GTAACCGAGG ACGACCTGTG GATCCGCACT 
TACGGCCGCC TCTTCCAGAA ACTCTGCTCC TCCAGCGCCG AGATCCCCAT CGGCATCTAC 
AGGACCGAGT GCCATGTCTT CTCGGAGCCC CATGACGTCA GAGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGACTGCGA GGATACTCGG GAGGCCAAGG GACCCTGGGG CACACGAGCT 
GCATCTGGCA GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC AGTGGAGCAC 
CCACTACTAC GTCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGTCGCAA GAGCACCAAG 
CAGGCAGGGA AGGCACCTGT GGCCACAGAC TGGATCACCC AGCAGCGGCT CAGCCTGTAC 
CGACGCTCAG AGCGCCAGGA GCTCTCAGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGA 
CTGCCCACCA CTGGCTATGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AACGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAACCCA ACGACATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA TGTGGCCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAGCTCT CATCCTGTAA TCCTGAGACC AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497944.2
CDS	57..3767
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497881.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497881.3

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCTGAAATGG ACGTCCAGGT GCGCAGGGTG 
CTGCAGATTC CCCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGATCTCA TGCGAGCCAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAG 
GACTTTGCCC CCAACTGTCC CCTCTATGTC CAGATCCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCACGT GGTATGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGTGGC 
CAGGAAGGAC AGGAGTCTCC CGAGCAGTGG CAGCGCACGT ACGGGAGGTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAATATGGGG TGTGCCTCAT CGGGCTGAAG 
CGTGAGGAGA ACAAGAGTAT CCTGCTGAAC CCAGGACCAC GGCACATCCT GGCTGCCTCC 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAACAG 
GAGGAGAAGC AGAAGAGACG GGGCCTTGCA GGGCAGGCAC TATATGAAGG GCCCTCCCGG 
CTCCCAGTGC ATAGCATCAT CGCCTCTATG GTGGCCATGG ACCTGCAGAA CACAGATTGC 
CGGCCCTCCC AGGGTGGCAG TGGCGGGGAC GGCACAAAGC TGACTCTGCC CACCGAGAAC 
GGCTCTGGTA GTCGACGCCC CAGCATCGCA CCCGTTCTGG AGTTGGCAGA CAGCTCAGCC 
CTGTTGCCCT GCGACCTGCT GAGTGACCAG TCAGAGGATG AGGTGACACC CTCGGACGAC 
GAGGGGCTCT CTGTGGTTGA GTACGTGAAG GGCTATCCCC CCAACTCACC CTACATTGGC 
AGCTCCCCGA CTTTATGCCA CCTCCTGCCT GTGAAAGCCC CCTTCTGCTG CCTGCGGTTG 
GACAAGGGCT GCAAACACAA CAGCTACGAG GATGCCAAGG CCTATGGGTT CAAGAACAAG 
CTGATTATTG TCTCTGCTGA GACGGCAGGC AACGGGCTCT ACAACTTCAT CGTGCCTCTG 
CGCGCCTACT ACCGGTCCCG CCGGGAGCTC AACCCTATCG TGTTGCTGCT TGACAACAAG 
CCTGACCACC ACTTCCTGGA GGCCATCTGT TGCTTCCCCA TGGTCTACTA CATGGAGGGA 
TCCGTGGACA ACCTGGACAG CTTACTGCAG TGTGGCATCA TCTATGCTGA CAACCTGGTG 
GTGGTGGACA AGGAGAGCAC TATGAGCGCT GAAGAGGACT ACATGGCAGA TGCCAAGACC 
ATCGTCAATG TGCAAACCAT GTTCCGGCTT TTCCCCAGTC TCAGCATCAC CACGGAGCTC 
ACACACCCTT CCAACATGCG GTTCATGCAG TTCCGTGCCA AGGACAGCTA CTCTCTGGCT 
CTTTCCAAAC TTGAAAAGCA AGAACGGGAG AACGGCTCCA ACCTGGCCTT CATGTTCCGC 
CTGCCATTTG CTGCTGGTCG AGTATTTAGT ATCAGCATGT TGGATACACT GCTCTACCAG 
TCCTTCGTGA AGGACTACAT GATCACCATC ACCAGGCTGC TCTTGGGCCT TGATACTACA 
CCAGGCTCCG GCTACCTCTG TGCAATGAAG GTAACCGAGG ACGACCTGTG GATCCGCACT 
TACGGCCGCC TCTTCCAGAA ACTCTGCTCC TCCAGCGCCG AGATCCCCAT CGGCATCTAC 
AGGACCGAGT GCCATGTCTT CTCGGAGCCC CATGACGTCA GAGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGACTGCGA GGATACTCGG GAGGCCAAGG GACCCTGGGG CACACGAGCT 
GCATCTGGCA GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC AGTGGAGCAC 
CCACTACTAC GTCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGTCGCAA GAGCACCAAG 
CAGGCAGGGA AGGCACCTGT GGCCACAGAC TGGATCACCC AGCAGCGGCT CAGCCTGTAC 
CGACGCTCAG AGCGCCAGGA GCTCTCAGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGA 
CTGCCCACCA CTGGCTATGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AACGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAACCCA ACGACATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA TGTGGCCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAGCTCT CATCCTGTAA TCCTGAGACC AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497882.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497945.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X6, mRNA.

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CloneID	OMu73351
Clone ID Related Accession (Same CDS sequence)	XM_006497882.3 , XM_006497882.4
Accession Version	XM_006497882.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497882.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GCAGGACTAC TACGTGGTCA TCCTGTGTCC CTCTGAAATG 
GACGTCCAGG TGCGCAGGGT GCTGCAGATT CCCCTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCTG CCCTCAAGGA CCAGGATCTC ATGCGAGCCA AGATGGACAA CGGAGAGGCC 
TGCTTTATCC TCAGCAGCAG GAATGAGGTG GACCGCACAG CTGCGGATCA CCAGACCATC 
CTTCGAGCCT GGGCTGTGAA GGACTTTGCC CCCAACTGTC CCCTCTATGT CCAGATCCTC 
AAGCCCGAAA ACAAGTTTCA CGTCAAATTT GCTGACCACG TGGTATGCGA GGAAGAGTGC 
AAGTACGCCA TGCTGGCCCT GAACTGCATC TGCCCGGCCA CCTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGTGG CCAGGAAGGA CAGGAGTCTC CCGAGCAGTG GCAGCGCACG 
TACGGGAGGT GCTCGGGCAA CGAGGTGTAC CACATTCGCA TGGGTGACAG TAAATTCTTC 
CGGGAGTATG AGGGCAAGAG CTTCACCTAC GCAGCCTTCC ACGCGCACAA GAAATATGGG 
GTGTGCCTCA TCGGGCTGAA GCGTGAGGAG AACAAGAGTA TCCTGCTGAA CCCAGGACCA 
CGGCACATCC TGGCTGCCTC CGACACCTGC TTCTATATCA ATATTACCAA GGAGGAGAAC 
TCAGCTTTCA TCTTCAAACA GGAGGAGAAG CAGAAGAGAC GGGGCCTTGC AGGGCAGGCA 
CTATATGAAG GGCCCTCCCG GCTCCCAGTG CATAGCATCA TCGCCTCTAT GGGGACAGTG 
GCCATGGACC TGCAGAACAC AGATTGCCGG CCCTCCCAGG GTGGCAGTGG CGGGGACGGC 
ACAAAGCTGA CTCTGCCCAC CGAGAACGGC TCTGGTAGTC GACGCCCCAG CATCGCACCC 
GTTCTGGAGT TGGCAGACAG CTCAGCCCTG TTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGATGAGG TGACACCCTC GGACGACGAG GGGCTCTCTG TGGTTGAGTA CGTGAAGGGC 
TATCCCCCCA ACTCACCCTA CATTGGCAGC TCCCCGACTT TATGCCACCT CCTGCCTGTG 
AAAGCCCCCT TCTGCTGCCT GCGGTTGGAC AAGGGCTGCA AACACAACAG CTACGAGGAT 
GCCAAGGCCT ATGGGTTCAA GAACAAGCTG ATTATTGTCT CTGCTGAGAC GGCAGGCAAC 
GGGCTCTACA ACTTCATCGT GCCTCTGCGC GCCTACTACC GGTCCCGCCG GGAGCTCAAC 
CCTATCGTGT TGCTGCTTGA CAACAAGCCT GACCACCACT TCCTGGAGGC CATCTGTTGC 
TTCCCCATGG TCTACTACAT GGAGGGATCC GTGGACAACC TGGACAGCTT ACTGCAGTGT 
GGCATCATCT ATGCTGACAA CCTGGTGGTG GTGGACAAGG AGAGCACTAT GAGCGCTGAA 
GAGGACTACA TGGCAGATGC CAAGACCATC GTCAATGTGC AAACCATGTT CCGGCTTTTC 
CCCAGTCTCA GCATCACCAC GGAGCTCACA CACCCTTCCA ACATGCGGTT CATGCAGTTC 
CGTGCCAAGG ACAGCTACTC TCTGGCTCTT TCCAAACTTG AAAAGCAAGA ACGGGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCATTTGCTG CTGGTCGAGT ATTTAGTATC 
AGCATGTTGG ATACACTGCT CTACCAGTCC TTCGTGAAGG ACTACATGAT CACCATCACC 
AGGCTGCTCT TGGGCCTTGA TACTACACCA GGCTCCGGCT ACCTCTGTGC AATGAAGGTA 
ACCGAGGACG ACCTGTGGAT CCGCACTTAC GGCCGCCTCT TCCAGAAACT CTGCTCCTCC 
AGCGCCGAGA TCCCCATCGG CATCTACAGG ACCGAGTGCC ATGTCTTCTC GGAGCCCCAT 
GACGTCAGAG CCCAGGGGGG CCCAGACATA GCCCTCCTGC CTGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGACTGCGA GGATACTCGG GAGGCCAAGG GACCCTGGGG CACACGAGCT 
GCATCTGGCA GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC AGTGGAGCAC 
CCACTACTAC GTCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGTCGCAA GAGCACCAAG 
CAGGCAGGGA AGGCACCTGT GGCCACAGAC TGGATCACCC AGCAGCGGCT CAGCCTGTAC 
CGACGCTCAG AGCGCCAGGA GCTCTCAGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGA 
CTGCCCACCA CTGGCTATGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AACGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAACCCA ACGACATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA TGTGGCCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAGCTCT CATCCTGTAA TCCTGAGACC AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497945.2
CDS	57..3767
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497882.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497882.3

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTATGTCAAT 
GAGAACACCT TCAAAGAGCG GCTCAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA TATCGTCCGT 
GTCCTGCTTG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACTTTCAATG GCTCATCCTC TGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
ATCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT CAACTGCTGG CTGGCCAAGC ATGCGCTGGA GAACATGATT 
AATGACTTCC ACCGTGCCAT CCTACGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATCCA GCACCTGGAG 
CGAGCAGGTG GCAACTTGAA CCTGCTGACC TCCTTCTACT TCTGCATCGT GACTTTCTCA 
ACTGTGGGCT TCGGTGATGT GACGCCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAATTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGTC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC GGAGAAGCAC 
GTAGTCCTGT GTGTGAGCTC CCTCAAGATT GATCTCCTCA TGGACTTCCT GAATGAGTTC 
TATGCCCATC CCCGGCTCCA GCAGGACTAC TACGTGGTCA TCCTGTGTCC CTCTGAAATG 
GACGTCCAGG TGCGCAGGGT GCTGCAGATT CCCCTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCTG CCCTCAAGGA CCAGGATCTC ATGCGAGCCA AGATGGACAA CGGAGAGGCC 
TGCTTTATCC TCAGCAGCAG GAATGAGGTG GACCGCACAG CTGCGGATCA CCAGACCATC 
CTTCGAGCCT GGGCTGTGAA GGACTTTGCC CCCAACTGTC CCCTCTATGT CCAGATCCTC 
AAGCCCGAAA ACAAGTTTCA CGTCAAATTT GCTGACCACG TGGTATGCGA GGAAGAGTGC 
AAGTACGCCA TGCTGGCCCT GAACTGCATC TGCCCGGCCA CCTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGTGG CCAGGAAGGA CAGGAGTCTC CCGAGCAGTG GCAGCGCACG 
TACGGGAGGT GCTCGGGCAA CGAGGTGTAC CACATTCGCA TGGGTGACAG TAAATTCTTC 
CGGGAGTATG AGGGCAAGAG CTTCACCTAC GCAGCCTTCC ACGCGCACAA GAAATATGGG 
GTGTGCCTCA TCGGGCTGAA GCGTGAGGAG AACAAGAGTA TCCTGCTGAA CCCAGGACCA 
CGGCACATCC TGGCTGCCTC CGACACCTGC TTCTATATCA ATATTACCAA GGAGGAGAAC 
TCAGCTTTCA TCTTCAAACA GGAGGAGAAG CAGAAGAGAC GGGGCCTTGC AGGGCAGGCA 
CTATATGAAG GGCCCTCCCG GCTCCCAGTG CATAGCATCA TCGCCTCTAT GGGGACAGTG 
GCCATGGACC TGCAGAACAC AGATTGCCGG CCCTCCCAGG GTGGCAGTGG CGGGGACGGC 
ACAAAGCTGA CTCTGCCCAC CGAGAACGGC TCTGGTAGTC GACGCCCCAG CATCGCACCC 
GTTCTGGAGT TGGCAGACAG CTCAGCCCTG TTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGATGAGG TGACACCCTC GGACGACGAG GGGCTCTCTG TGGTTGAGTA CGTGAAGGGC 
TATCCCCCCA ACTCACCCTA CATTGGCAGC TCCCCGACTT TATGCCACCT CCTGCCTGTG 
AAAGCCCCCT TCTGCTGCCT GCGGTTGGAC AAGGGCTGCA AACACAACAG CTACGAGGAT 
GCCAAGGCCT ATGGGTTCAA GAACAAGCTG ATTATTGTCT CTGCTGAGAC GGCAGGCAAC 
GGGCTCTACA ACTTCATCGT GCCTCTGCGC GCCTACTACC GGTCCCGCCG GGAGCTCAAC 
CCTATCGTGT TGCTGCTTGA CAACAAGCCT GACCACCACT TCCTGGAGGC CATCTGTTGC 
TTCCCCATGG TCTACTACAT GGAGGGATCC GTGGACAACC TGGACAGCTT ACTGCAGTGT 
GGCATCATCT ATGCTGACAA CCTGGTGGTG GTGGACAAGG AGAGCACTAT GAGCGCTGAA 
GAGGACTACA TGGCAGATGC CAAGACCATC GTCAATGTGC AAACCATGTT CCGGCTTTTC 
CCCAGTCTCA GCATCACCAC GGAGCTCACA CACCCTTCCA ACATGCGGTT CATGCAGTTC 
CGTGCCAAGG ACAGCTACTC TCTGGCTCTT TCCAAACTTG AAAAGCAAGA ACGGGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCATTTGCTG CTGGTCGAGT ATTTAGTATC 
AGCATGTTGG ATACACTGCT CTACCAGTCC TTCGTGAAGG ACTACATGAT CACCATCACC 
AGGCTGCTCT TGGGCCTTGA TACTACACCA GGCTCCGGCT ACCTCTGTGC AATGAAGGTA 
ACCGAGGACG ACCTGTGGAT CCGCACTTAC GGCCGCCTCT TCCAGAAACT CTGCTCCTCC 
AGCGCCGAGA TCCCCATCGG CATCTACAGG ACCGAGTGCC ATGTCTTCTC GGAGCCCCAT 
GACGTCAGAG CCCAGGGGGG CCCAGACATA GCCCTCCTGC CTGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGACTGCGA GGATACTCGG GAGGCCAAGG GACCCTGGGG CACACGAGCT 
GCATCTGGCA GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC AGTGGAGCAC 
CCACTACTAC GTCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGTCGCAA GAGCACCAAG 
CAGGCAGGGA AGGCACCTGT GGCCACAGAC TGGATCACCC AGCAGCGGCT CAGCCTGTAC 
CGACGCTCAG AGCGCCAGGA GCTCTCAGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGA 
CTGCCCACCA CTGGCTATGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AACGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAACCCA ACGACATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA TGTGGCCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAGCTCT CATCCTGTAA TCCTGAGACC AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497883.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497946.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X7, mRNA.

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CloneID	OMu73352
Clone ID Related Accession (Same CDS sequence)	XM_006497883.4 , XM_006497883.3
Accession Version	XM_006497883.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497883.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGT CCTTCGTGAA GGACTACATG ATCACCATCA CCAGGCTGCT CTTGGGCCTT 
GATACTACAC CAGGCTCCGG CTACCTCTGT GCAATGAAGG TAACCGAGGA CGACCTGTGG 
ATCCGCACTT ACGGCCGCCT CTTCCAGAAA CTCTGCTCCT CCAGCGCCGA GATCCCCATC 
GGCATCTACA GGACCGAGTG CCATGTCTTC TCGGAGCCCC ATGACGTCAG AGCCCAGGGG 
GGCCCAGACA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGACTGC 
GAGGATACTC GGGAGGCCAA GGGACCCTGG GGCACACGAG CTGCATCTGG CAGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCAGTGGAGC ACCCACTACT ACGTCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGTCGC AAGAGCACCA AGCAGGCAGG GAAGGCACCT 
GTGGCCACAG ACTGGATCAC CCAGCAGCGG CTCAGCCTGT ACCGACGCTC AGAGCGCCAG 
GAGCTCTCAG AGCTGGTCAA GAACCGAATG AAGCACCTGG GACTGCCCAC CACTGGCTAT 
GATGAGATGA ACGATCATCA CCAGAACACC CTTTCCTATG TACTCATCAA CCCCCCGCCA 
GACACAAGAC TGGAACCCAA CGACATTGTG TACCTCATCC GTTCCGACCC CCTGGCCCAT 
GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT 
CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497946.2
CDS	204..3893
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497883.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497883.3

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAAGC CTGACCACCA CTTCCTGGAG GCCATCTGTT GCTTCCCCAT GGTCTACTAC 
ATGGAGGGAT CCGTGGACAA CCTGGACAGC TTACTGCAGT GTGGCATCAT CTATGCTGAC 
AACCTGGTGG TGGTGGACAA GGAGAGCACT ATGAGCGCTG AAGAGGACTA CATGGCAGAT 
GCCAAGACCA TCGTCAATGT GCAAACCATG TTCCGGCTTT TCCCCAGTCT CAGCATCACC 
ACGGAGCTCA CACACCCTTC CAACATGCGG TTCATGCAGT TCCGTGCCAA GGACAGCTAC 
TCTCTGGCTC TTTCCAAACT TGAAAAGCAA GAACGGGAGA ACGGCTCCAA CCTGGCCTTC 
ATGTTCCGCC TGCCATTTGC TGCTGGTCGA GTATTTAGTA TCAGCATGTT GGATACACTG 
CTCTACCAGT CCTTCGTGAA GGACTACATG ATCACCATCA CCAGGCTGCT CTTGGGCCTT 
GATACTACAC CAGGCTCCGG CTACCTCTGT GCAATGAAGG TAACCGAGGA CGACCTGTGG 
ATCCGCACTT ACGGCCGCCT CTTCCAGAAA CTCTGCTCCT CCAGCGCCGA GATCCCCATC 
GGCATCTACA GGACCGAGTG CCATGTCTTC TCGGAGCCCC ATGACGTCAG AGCCCAGGGG 
GGCCCAGACA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGACTGC 
GAGGATACTC GGGAGGCCAA GGGACCCTGG GGCACACGAG CTGCATCTGG CAGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCAGTGGAGC ACCCACTACT ACGTCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGTCGC AAGAGCACCA AGCAGGCAGG GAAGGCACCT 
GTGGCCACAG ACTGGATCAC CCAGCAGCGG CTCAGCCTGT ACCGACGCTC AGAGCGCCAG 
GAGCTCTCAG AGCTGGTCAA GAACCGAATG AAGCACCTGG GACTGCCCAC CACTGGCTAT 
GATGAGATGA ACGATCATCA CCAGAACACC CTTTCCTATG TACTCATCAA CCCCCCGCCA 
GACACAAGAC TGGAACCCAA CGACATTGTG TACCTCATCC GTTCCGACCC CCTGGCCCAT 
GTGGCCAGCA GCTCCCAGAG TCGGAAAAGC AGCTGCAGCA ACAAGCTCTC ATCCTGTAAT 
CCTGAGACCA GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497884.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497947.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu73353
Clone ID Related Accession (Same CDS sequence)	XM_006497884.4 , XM_006497884.3
Accession Version	XM_006497884.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497884.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAACC TGGACAGCTT ACTGCAGTGT GGCATCATCT ATGCTGACAA CCTGGTGGTG 
GTGGACAAGG AGAGCACTAT GAGCGCTGAA GAGGACTACA TGGCAGATGC CAAGACCATC 
GTCAATGTGC AAACCATGTT CCGGCTTTTC CCCAGTCTCA GCATCACCAC GGAGCTCACA 
CACCCTTCCA ACATGCGGTT CATGCAGTTC CGTGCCAAGG ACAGCTACTC TCTGGCTCTT 
TCCAAACTTG AAAAGCAAGA ACGGGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG 
CCATTTGCTG CTGGTCGAGT ATTTAGTATC AGCATGTTGG ATACACTGCT CTACCAGTCC 
TTCGTGAAGG ACTACATGAT CACCATCACC AGGCTGCTCT TGGGCCTTGA TACTACACCA 
GGCTCCGGCT ACCTCTGTGC AATGAAGGTA ACCGAGGACG ACCTGTGGAT CCGCACTTAC 
GGCCGCCTCT TCCAGAAACT CTGCTCCTCC AGCGCCGAGA TCCCCATCGG CATCTACAGG 
ACCGAGTGCC ATGTCTTCTC GGAGCCCCAT GACGTCAGAG CCCAGGGGGG CCCAGACATA 
GCCCTCCTGC CTGCCCAGTC TCAGATCTCG GTGAACATGG AGGACTGCGA GGATACTCGG 
GAGGCCAAGG GACCCTGGGG CACACGAGCT GCATCTGGCA GTGGCAGCAC CCATGGCCGT 
CACGGGGGCA GTGCTGACCC AGTGGAGCAC CCACTACTAC GTCGCAAGAG CCTGCAGTGG 
GCCCGCAAGC TGAGTCGCAA GAGCACCAAG CAGGCAGGGA AGGCACCTGT GGCCACAGAC 
TGGATCACCC AGCAGCGGCT CAGCCTGTAC CGACGCTCAG AGCGCCAGGA GCTCTCAGAG 
CTGGTCAAGA ACCGAATGAA GCACCTGGGA CTGCCCACCA CTGGCTATGA GGACGTAGCA 
AATTTAACAG CCAGTGATGT GATGAATCGG GTAAACCTGG GATATTTGCA AGATGAGATG 
AACGATCATC ACCAGAACAC CCTTTCCTAT GTACTCATCA ACCCCCCGCC AGACACAAGA 
CTGGAACCCA ACGACATTGT GTACCTCATC CGTTCCGACC CCCTGGCCCA TGTGGCCAGC 
AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC AACAAGCTCT CATCCTGTAA TCCTGAGACC 
AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497947.2
CDS	203..3883
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497884.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497884.3

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGACCA CGTGGTATGC GAGGAAGAGT GCAAGTACGC CATGCTGGCC 
CTGAACTGCA TCTGCCCGGC CACCTCCACC CTCATCACCC TGCTGGTGCA CACGTCCCGT 
GGCCAGGAAG GACAGGAGTC TCCCGAGCAG TGGCAGCGCA CGTACGGGAG GTGCTCGGGC 
AACGAGGTGT ACCACATTCG CATGGGTGAC AGTAAATTCT TCCGGGAGTA TGAGGGCAAG 
AGCTTCACCT ACGCAGCCTT CCACGCGCAC AAGAAATATG GGGTGTGCCT CATCGGGCTG 
AAGCGTGAGG AGAACAAGAG TATCCTGCTG AACCCAGGAC CACGGCACAT CCTGGCTGCC 
TCCGACACCT GCTTCTATAT CAATATTACC AAGGAGGAGA ACTCAGCTTT CATCTTCAAA 
CAGGAGGAGA AGCAGAAGAG ACGGGGCCTT GCAGGGCAGG CACTATATGA AGGGCCCTCC 
CGGCTCCCAG TGCATAGCAT CATCGCCTCT ATGGGGACAG TGGCCATGGA CCTGCAGAAC 
ACAGATTGCC GGCCCTCCCA GGGTGGCAGT GGCGGGGACG GCACAAAGCT GACTCTGCCC 
ACCGAGAACG GCTCTGGTAG TCGACGCCCC AGCATCGCAC CCGTTCTGGA GTTGGCAGAC 
AGCTCAGCCC TGTTGCCCTG CGACCTGCTG AGTGACCAGT CAGAGGATGA GGTGACACCC 
TCGGACGACG AGGGGCTCTC TGTGGTTGAG TACGTGAAGG GCTATCCCCC CAACTCACCC 
TACATTGGCA GCTCCCCGAC TTTATGCCAC CTCCTGCCTG TGAAAGCCCC CTTCTGCTGC 
CTGCGGTTGG ACAAGGGCTG CAAACACAAC AGCTACGAGG ATGCCAAGGC CTATGGGTTC 
AAGAACAAGC TGATTATTGT CTCTGCTGAG ACGGCAGGCA ACGGGCTCTA CAACTTCATC 
GTGCCTCTGC GCGCCTACTA CCGGTCCCGC CGGGAGCTCA ACCCTATCGT GTTGCTGCTT 
GACAACAACC TGGACAGCTT ACTGCAGTGT GGCATCATCT ATGCTGACAA CCTGGTGGTG 
GTGGACAAGG AGAGCACTAT GAGCGCTGAA GAGGACTACA TGGCAGATGC CAAGACCATC 
GTCAATGTGC AAACCATGTT CCGGCTTTTC CCCAGTCTCA GCATCACCAC GGAGCTCACA 
CACCCTTCCA ACATGCGGTT CATGCAGTTC CGTGCCAAGG ACAGCTACTC TCTGGCTCTT 
TCCAAACTTG AAAAGCAAGA ACGGGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG 
CCATTTGCTG CTGGTCGAGT ATTTAGTATC AGCATGTTGG ATACACTGCT CTACCAGTCC 
TTCGTGAAGG ACTACATGAT CACCATCACC AGGCTGCTCT TGGGCCTTGA TACTACACCA 
GGCTCCGGCT ACCTCTGTGC AATGAAGGTA ACCGAGGACG ACCTGTGGAT CCGCACTTAC 
GGCCGCCTCT TCCAGAAACT CTGCTCCTCC AGCGCCGAGA TCCCCATCGG CATCTACAGG 
ACCGAGTGCC ATGTCTTCTC GGAGCCCCAT GACGTCAGAG CCCAGGGGGG CCCAGACATA 
GCCCTCCTGC CTGCCCAGTC TCAGATCTCG GTGAACATGG AGGACTGCGA GGATACTCGG 
GAGGCCAAGG GACCCTGGGG CACACGAGCT GCATCTGGCA GTGGCAGCAC CCATGGCCGT 
CACGGGGGCA GTGCTGACCC AGTGGAGCAC CCACTACTAC GTCGCAAGAG CCTGCAGTGG 
GCCCGCAAGC TGAGTCGCAA GAGCACCAAG CAGGCAGGGA AGGCACCTGT GGCCACAGAC 
TGGATCACCC AGCAGCGGCT CAGCCTGTAC CGACGCTCAG AGCGCCAGGA GCTCTCAGAG 
CTGGTCAAGA ACCGAATGAA GCACCTGGGA CTGCCCACCA CTGGCTATGA GGACGTAGCA 
AATTTAACAG CCAGTGATGT GATGAATCGG GTAAACCTGG GATATTTGCA AGATGAGATG 
AACGATCATC ACCAGAACAC CCTTTCCTAT GTACTCATCA ACCCCCCGCC AGACACAAGA 
CTGGAACCCA ACGACATTGT GTACCTCATC CGTTCCGACC CCCTGGCCCA TGTGGCCAGC 
AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC AACAAGCTCT CATCCTGTAA TCCTGAGACC 
AGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497886.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497949.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X10, mRNA.

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CloneID	OMu73354
Clone ID Related Accession (Same CDS sequence)	XM_006497886.3 , XM_006497886.4
Accession Version	XM_006497886.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGAA TGACTTCCAC CGTGCCATCC TACGCACACA GTCAGCCATG 
TTCAACCAGG TGCTCATCCT GTTCTGCACC CTGCTGTGCC TGGTCTTCAC AGGGACCTGT 
GGGATCCAGC ACCTGGAGCG AGCAGGTGGC AACTTGAACC TGCTGACCTC CTTCTACTTC 
TGCATCGTGA CTTTCTCAAC TGTGGGCTTC GGTGATGTGA CGCCCAAGAT CTGGCCATCC 
CAGCTCCTGG TGGTCATCCT GATCTGTGTC ACCCTTGTGG TGCTCCCACT GCAATTTGAA 
GAGCTTGTCT ACCTCTGGAT GGAGCGTCAG AAGTCAGGGG GCAACTATAG CCGCCACCGA 
GCACGGACGG AGAAGCACGT AGTCCTGTGT GTGAGCTCCC TCAAGATTGA TCTCCTCATG 
GACTTCCTGA ATGAGTTCTA TGCCCATCCC CGGCTCCAGC AGGACTACTA CGTGGTCATC 
CTGTGTCCCT CTGAAATGGA CGTCCAGGTG CGCAGGGTGC TGCAGATTCC CCTGTGGTCC 
CAGCGGGTCA TCTACCTCCA GGGCTCTGCC CTCAAGGACC AGGATCTCAT GCGAGCCAAG 
ATGGACAACG GAGAGGCCTG CTTTATCCTC AGCAGCAGGA ATGAGGTGGA CCGCACAGCT 
GCGGATCACC AGACCATCCT TCGAGCCTGG GCTGTGAAGG ACTTTGCCCC CAACTGTCCC 
CTCTATGTCC AGATCCTCAA GCCCGAAAAC AAGTTTCACG TCAAATTTGC TGACCACGTG 
GTATGCGAGG AAGAGTGCAA GTACGCCATG CTGGCCCTGA ACTGCATCTG CCCGGCCACC 
TCCACCCTCA TCACCCTGCT GGTGCACACG TCCCGTGGCC AGGAAGGACA GGAGTCTCCC 
GAGCAGTGGC AGCGCACGTA CGGGAGGTGC TCGGGCAACG AGGTGTACCA CATTCGCATG 
GGTGACAGTA AATTCTTCCG GGAGTATGAG GGCAAGAGCT TCACCTACGC AGCCTTCCAC 
GCGCACAAGA AATATGGGGT GTGCCTCATC GGGCTGAAGC GTGAGGAGAA CAAGAGTATC 
CTGCTGAACC CAGGACCACG GCACATCCTG GCTGCCTCCG ACACCTGCTT CTATATCAAT 
ATTACCAAGG AGGAGAACTC AGCTTTCATC TTCAAACAGG AGGAGAAGCA GAAGAGACGG 
GGCCTTGCAG GGCAGGCACT ATATGAAGGG CCCTCCCGGC TCCCAGTGCA TAGCATCATC 
GCCTCTATGG GGACAGTGGC CATGGACCTG CAGAACACAG ATTGCCGGCC CTCCCAGGGT 
GGCAGTGGCG GGGACGGCAC AAAGCTGACT CTGCCCACCG AGAACGGCTC TGGTAGTCGA 
CGCCCCAGCA TCGCACCCGT TCTGGAGTTG GCAGACAGCT CAGCCCTGTT GCCCTGCGAC 
CTGCTGAGTG ACCAGTCAGA GGATGAGGTG ACACCCTCGG ACGACGAGGG GCTCTCTGTG 
GTTGAGTACG TGAAGGGCTA TCCCCCCAAC TCACCCTACA TTGGCAGCTC CCCGACTTTA 
TGCCACCTCC TGCCTGTGAA AGCCCCCTTC TGCTGCCTGC GGTTGGACAA GGGCTGCAAA 
CACAACAGCT ACGAGGATGC CAAGGCCTAT GGGTTCAAGA ACAAGCTGAT TATTGTCTCT 
GCTGAGACGG CAGGCAACGG GCTCTACAAC TTCATCGTGC CTCTGCGCGC CTACTACCGG 
TCCCGCCGGG AGCTCAACCC TATCGTGTTG CTGCTTGACA ACAAGCCTGA CCACCACTTC 
CTGGAGGCCA TCTGTTGCTT CCCCATGGTC TACTACATGG AGGGATCCGT GGACAACCTG 
GACAGCTTAC TGCAGTGTGG CATCATCTAT GCTGACAACC TGGTGGTGGT GGACAAGGAG 
AGCACTATGA GCGCTGAAGA GGACTACATG GCAGATGCCA AGACCATCGT CAATGTGCAA 
ACCATGTTCC GGCTTTTCCC CAGTCTCAGC ATCACCACGG AGCTCACACA CCCTTCCAAC 
ATGCGGTTCA TGCAGTTCCG TGCCAAGGAC AGCTACTCTC TGGCTCTTTC CAAACTTGAA 
AAGCAAGAAC GGGAGAACGG CTCCAACCTG GCCTTCATGT TCCGCCTGCC ATTTGCTGCT 
GGTCGAGTAT TTAGTATCAG CATGTTGGAT ACACTGCTCT ACCAGTCCTT CGTGAAGGAC 
TACATGATCA CCATCACCAG GCTGCTCTTG GGCCTTGATA CTACACCAGG CTCCGGCTAC 
CTCTGTGCAA TGAAGGTAAC CGAGGACGAC CTGTGGATCC GCACTTACGG CCGCCTCTTC 
CAGAAACTCT GCTCCTCCAG CGCCGAGATC CCCATCGGCA TCTACAGGAC CGAGTGCCAT 
GTCTTCTCGG AGCCCCATGA CGTCAGAGCC CAGGGGGGCC CAGACATAGC CCTCCTGCCT 
GCCCAGTCTC AGATCTCGGT GAACATGGAG GACTGCGAGG ATACTCGGGA GGCCAAGGGA 
CCCTGGGGCA CACGAGCTGC ATCTGGCAGT GGCAGCACCC ATGGCCGTCA CGGGGGCAGT 
GCTGACCCAG TGGAGCACCC ACTACTACGT CGCAAGAGCC TGCAGTGGGC CCGCAAGCTG 
AGTCGCAAGA GCACCAAGCA GGCAGGGAAG GCACCTGTGG CCACAGACTG GATCACCCAG 
CAGCGGCTCA GCCTGTACCG ACGCTCAGAG CGCCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGAATGAAGC ACCTGGGACT GCCCACCACT GGCTATGAGG ACGTAGCAAA TTTAACAGCC 
AGTGATGTGA TGAATCGGGT AAACCTGGGA TATTTGCAAG ATGAGATGAA CGATCATCAC 
CAGAACACCC TTTCCTATGT ACTCATCAAC CCCCCGCCAG ACACAAGACT GGAACCCAAC 
GACATTGTGT ACCTCATCCG TTCCGACCCC CTGGCCCATG TGGCCAGCAG CTCCCAGAGT 
CGGAAAAGCA GCTGCAGCAA CAAGCTCTCA TCCTGTAATC CTGAGACCAG GGATGAGACC 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497949.2
CDS	59..3727
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITNDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497886.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497886.3

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGAA TGACTTCCAC CGTGCCATCC TACGCACACA GTCAGCCATG 
TTCAACCAGG TGCTCATCCT GTTCTGCACC CTGCTGTGCC TGGTCTTCAC AGGGACCTGT 
GGGATCCAGC ACCTGGAGCG AGCAGGTGGC AACTTGAACC TGCTGACCTC CTTCTACTTC 
TGCATCGTGA CTTTCTCAAC TGTGGGCTTC GGTGATGTGA CGCCCAAGAT CTGGCCATCC 
CAGCTCCTGG TGGTCATCCT GATCTGTGTC ACCCTTGTGG TGCTCCCACT GCAATTTGAA 
GAGCTTGTCT ACCTCTGGAT GGAGCGTCAG AAGTCAGGGG GCAACTATAG CCGCCACCGA 
GCACGGACGG AGAAGCACGT AGTCCTGTGT GTGAGCTCCC TCAAGATTGA TCTCCTCATG 
GACTTCCTGA ATGAGTTCTA TGCCCATCCC CGGCTCCAGC AGGACTACTA CGTGGTCATC 
CTGTGTCCCT CTGAAATGGA CGTCCAGGTG CGCAGGGTGC TGCAGATTCC CCTGTGGTCC 
CAGCGGGTCA TCTACCTCCA GGGCTCTGCC CTCAAGGACC AGGATCTCAT GCGAGCCAAG 
ATGGACAACG GAGAGGCCTG CTTTATCCTC AGCAGCAGGA ATGAGGTGGA CCGCACAGCT 
GCGGATCACC AGACCATCCT TCGAGCCTGG GCTGTGAAGG ACTTTGCCCC CAACTGTCCC 
CTCTATGTCC AGATCCTCAA GCCCGAAAAC AAGTTTCACG TCAAATTTGC TGACCACGTG 
GTATGCGAGG AAGAGTGCAA GTACGCCATG CTGGCCCTGA ACTGCATCTG CCCGGCCACC 
TCCACCCTCA TCACCCTGCT GGTGCACACG TCCCGTGGCC AGGAAGGACA GGAGTCTCCC 
GAGCAGTGGC AGCGCACGTA CGGGAGGTGC TCGGGCAACG AGGTGTACCA CATTCGCATG 
GGTGACAGTA AATTCTTCCG GGAGTATGAG GGCAAGAGCT TCACCTACGC AGCCTTCCAC 
GCGCACAAGA AATATGGGGT GTGCCTCATC GGGCTGAAGC GTGAGGAGAA CAAGAGTATC 
CTGCTGAACC CAGGACCACG GCACATCCTG GCTGCCTCCG ACACCTGCTT CTATATCAAT 
ATTACCAAGG AGGAGAACTC AGCTTTCATC TTCAAACAGG AGGAGAAGCA GAAGAGACGG 
GGCCTTGCAG GGCAGGCACT ATATGAAGGG CCCTCCCGGC TCCCAGTGCA TAGCATCATC 
GCCTCTATGG GGACAGTGGC CATGGACCTG CAGAACACAG ATTGCCGGCC CTCCCAGGGT 
GGCAGTGGCG GGGACGGCAC AAAGCTGACT CTGCCCACCG AGAACGGCTC TGGTAGTCGA 
CGCCCCAGCA TCGCACCCGT TCTGGAGTTG GCAGACAGCT CAGCCCTGTT GCCCTGCGAC 
CTGCTGAGTG ACCAGTCAGA GGATGAGGTG ACACCCTCGG ACGACGAGGG GCTCTCTGTG 
GTTGAGTACG TGAAGGGCTA TCCCCCCAAC TCACCCTACA TTGGCAGCTC CCCGACTTTA 
TGCCACCTCC TGCCTGTGAA AGCCCCCTTC TGCTGCCTGC GGTTGGACAA GGGCTGCAAA 
CACAACAGCT ACGAGGATGC CAAGGCCTAT GGGTTCAAGA ACAAGCTGAT TATTGTCTCT 
GCTGAGACGG CAGGCAACGG GCTCTACAAC TTCATCGTGC CTCTGCGCGC CTACTACCGG 
TCCCGCCGGG AGCTCAACCC TATCGTGTTG CTGCTTGACA ACAAGCCTGA CCACCACTTC 
CTGGAGGCCA TCTGTTGCTT CCCCATGGTC TACTACATGG AGGGATCCGT GGACAACCTG 
GACAGCTTAC TGCAGTGTGG CATCATCTAT GCTGACAACC TGGTGGTGGT GGACAAGGAG 
AGCACTATGA GCGCTGAAGA GGACTACATG GCAGATGCCA AGACCATCGT CAATGTGCAA 
ACCATGTTCC GGCTTTTCCC CAGTCTCAGC ATCACCACGG AGCTCACACA CCCTTCCAAC 
ATGCGGTTCA TGCAGTTCCG TGCCAAGGAC AGCTACTCTC TGGCTCTTTC CAAACTTGAA 
AAGCAAGAAC GGGAGAACGG CTCCAACCTG GCCTTCATGT TCCGCCTGCC ATTTGCTGCT 
GGTCGAGTAT TTAGTATCAG CATGTTGGAT ACACTGCTCT ACCAGTCCTT CGTGAAGGAC 
TACATGATCA CCATCACCAG GCTGCTCTTG GGCCTTGATA CTACACCAGG CTCCGGCTAC 
CTCTGTGCAA TGAAGGTAAC CGAGGACGAC CTGTGGATCC GCACTTACGG CCGCCTCTTC 
CAGAAACTCT GCTCCTCCAG CGCCGAGATC CCCATCGGCA TCTACAGGAC CGAGTGCCAT 
GTCTTCTCGG AGCCCCATGA CGTCAGAGCC CAGGGGGGCC CAGACATAGC CCTCCTGCCT 
GCCCAGTCTC AGATCTCGGT GAACATGGAG GACTGCGAGG ATACTCGGGA GGCCAAGGGA 
CCCTGGGGCA CACGAGCTGC ATCTGGCAGT GGCAGCACCC ATGGCCGTCA CGGGGGCAGT 
GCTGACCCAG TGGAGCACCC ACTACTACGT CGCAAGAGCC TGCAGTGGGC CCGCAAGCTG 
AGTCGCAAGA GCACCAAGCA GGCAGGGAAG GCACCTGTGG CCACAGACTG GATCACCCAG 
CAGCGGCTCA GCCTGTACCG ACGCTCAGAG CGCCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGAATGAAGC ACCTGGGACT GCCCACCACT GGCTATGAGG ACGTAGCAAA TTTAACAGCC 
AGTGATGTGA TGAATCGGGT AAACCTGGGA TATTTGCAAG ATGAGATGAA CGATCATCAC 
CAGAACACCC TTTCCTATGT ACTCATCAAC CCCCCGCCAG ACACAAGACT GGAACCCAAC 
GACATTGTGT ACCTCATCCG TTCCGACCCC CTGGCCCATG TGGCCAGCAG CTCCCAGAGT 
CGGAAAAGCA GCTGCAGCAA CAAGCTCTCA TCCTGTAATC CTGAGACCAG GGATGAGACC 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnt1 ( XM_006497889.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497952.2 Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X15, mRNA.

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CloneID	OMu73355
Clone ID Related Accession (Same CDS sequence)	XM_006497889.4 , XM_006497889.3
Accession Version	XM_006497889.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily T member 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497889.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGGGAC AGTGGCCATG GACCTGCAGA ACACAGATTG CCGGCCCTCC 
CAGGGTGGCA GTGGCGGGGA CGGCACAAAG CTGACTCTGC CCACCGAGAA CGGCTCTGGT 
AGTCGACGCC CCAGCATCGC ACCCGTTCTG GAGTTGGCAG ACAGCTCAGC CCTGTTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAT GAGGTGACAC CCTCGGACGA CGAGGGGCTC 
TCTGTGGTTG AGTACGTGAA GGGCTATCCC CCCAACTCAC CCTACATTGG CAGCTCCCCG 
ACTTTATGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGTT GGACAAGGGC 
TGCAAACACA ACAGCTACGA GGATGCCAAG GCCTATGGGT TCAAGAACAA GCTGATTATT 
GTCTCTGCTG AGACGGCAGG CAACGGGCTC TACAACTTCA TCGTGCCTCT GCGCGCCTAC 
TACCGGTCCC GCCGGGAGCT CAACCCTATC GTGTTGCTGC TTGACAACAA GCCTGACCAC 
CACTTCCTGG AGGCCATCTG TTGCTTCCCC ATGGTCTACT ACATGGAGGG ATCCGTGGAC 
AACCTGGACA GCTTACTGCA GTGTGGCATC ATCTATGCTG ACAACCTGGT GGTGGTGGAC 
AAGGAGAGCA CTATGAGCGC TGAAGAGGAC TACATGGCAG ATGCCAAGAC CATCGTCAAT 
GTGCAAACCA TGTTCCGGCT TTTCCCCAGT CTCAGCATCA CCACGGAGCT CACACACCCT 
TCCAACATGC GGTTCATGCA GTTCCGTGCC AAGGACAGCT ACTCTCTGGC TCTTTCCAAA 
CTTGAAAAGC AAGAACGGGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCATTT 
GCTGCTGGTC GAGTATTTAG TATCAGCATG TTGGATACAC TGCTCTACCA GTCCTTCGTG 
AAGGACTACA TGATCACCAT CACCAGGCTG CTCTTGGGCC TTGATACTAC ACCAGGCTCC 
GGCTACCTCT GTGCAATGAA GGTAACCGAG GACGACCTGT GGATCCGCAC TTACGGCCGC 
CTCTTCCAGA AACTCTGCTC CTCCAGCGCC GAGATCCCCA TCGGCATCTA CAGGACCGAG 
TGCCATGTCT TCTCGGAGCC CCATGACGTC AGAGCCCAGG GGGGCCCAGA CATAGCCCTC 
CTGCCTGCCC AGTCTCAGAT CTCGGTGAAC ATGGAGGACT GCGAGGATAC TCGGGAGGCC 
AAGGGACCCT GGGGCACACG AGCTGCATCT GGCAGTGGCA GCACCCATGG CCGTCACGGG 
GGCAGTGCTG ACCCAGTGGA GCACCCACTA CTACGTCGCA AGAGCCTGCA GTGGGCCCGC 
AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA GGGAAGGCAC CTGTGGCCAC AGACTGGATC 
ACCCAGCAGC GGCTCAGCCT GTACCGACGC TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGAA TGAAGCACCT GGGACTGCCC ACCACTGGCT ATGAGGACGT AGCAAATTTA 
ACAGCCAGTG ATGTGATGAA TCGGGTAAAC CTGGGATATT TGCAAGATGA GATGAACGAT 
CATCACCAGA ACACCCTTTC CTATGTACTC ATCAACCCCC CGCCAGACAC AAGACTGGAA 
CCCAACGACA TTGTGTACCT CATCCGTTCC GACCCCCTGG CCCATGTGGC CAGCAGCTCC 
CAGAGTCGGA AAAGCAGCTG CAGCAACAAG CTCTCATCCT GTAATCCTGA GACCAGGGAT 
GAGACCCAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497952.2
CDS	59..3313
Translation	MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQGGPDIALLPAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006497889.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily T, member 1 (Kcnt1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497889.3

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCG CTGCAGCCCC TGAGGAGCCA CACGGGCTCA GCCCGCTACT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAG TTCTATGTCA ATGAGAACAC CTTCAAAGAG 
CGGCTCAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TATATCGTCC GTGTCCTGCT TGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACTTTCAA TGGCTCATCC 
TCTGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCATCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTCAACTGCT GGCTGGCCAA GCATGCGCTG GAGAACATGA TTAATGACTT CCACCGTGCC 
ATCCTACGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATC CAGCACCTGG AGCGAGCAGG TGGCAACTTG 
AACCTGCTGA CCTCCTTCTA CTTCTGCATC GTGACTTTCT CAACTGTGGG CTTCGGTGAT 
GTGACGCCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAATT TGAAGAGCTT GTCTACCTCT GGATGGAGCG TCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACGGAGAAGC ACGTAGTCCT GTGTGTGAGC 
TCCCTCAAGA TTGATCTCCT CATGGACTTC CTGAATGAGT TCTATGCCCA TCCCCGGCTC 
CAGCAGGACT ACTACGTGGT CATCCTGTGT CCCTCTGAAA TGGACGTCCA GGTGCGCAGG 
GTGCTGCAGA TTCCCCTGTG GTCCCAGCGG GTCATCTACC TCCAGGGCTC TGCCCTCAAG 
GACCAGGATC TCATGCGAGC CAAGATGGAC AACGGAGAGG CCTGCTTTAT CCTCAGCAGC 
AGGAATGAGG TGGACCGCAC AGCTGCGGAT CACCAGACCA TCCTTCGAGC CTGGGCTGTG 
AAGGACTTTG CCCCCAACTG TCCCCTCTAT GTCCAGATCC TCAAGCCCGA AAACAAGTTT 
CACGTCAAAT TTGCTGGGAC AGTGGCCATG GACCTGCAGA ACACAGATTG CCGGCCCTCC 
CAGGGTGGCA GTGGCGGGGA CGGCACAAAG CTGACTCTGC CCACCGAGAA CGGCTCTGGT 
AGTCGACGCC CCAGCATCGC ACCCGTTCTG GAGTTGGCAG ACAGCTCAGC CCTGTTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAT GAGGTGACAC CCTCGGACGA CGAGGGGCTC 
TCTGTGGTTG AGTACGTGAA GGGCTATCCC CCCAACTCAC CCTACATTGG CAGCTCCCCG 
ACTTTATGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGTT GGACAAGGGC 
TGCAAACACA ACAGCTACGA GGATGCCAAG GCCTATGGGT TCAAGAACAA GCTGATTATT 
GTCTCTGCTG AGACGGCAGG CAACGGGCTC TACAACTTCA TCGTGCCTCT GCGCGCCTAC 
TACCGGTCCC GCCGGGAGCT CAACCCTATC GTGTTGCTGC TTGACAACAA GCCTGACCAC 
CACTTCCTGG AGGCCATCTG TTGCTTCCCC ATGGTCTACT ACATGGAGGG ATCCGTGGAC 
AACCTGGACA GCTTACTGCA GTGTGGCATC ATCTATGCTG ACAACCTGGT GGTGGTGGAC 
AAGGAGAGCA CTATGAGCGC TGAAGAGGAC TACATGGCAG ATGCCAAGAC CATCGTCAAT 
GTGCAAACCA TGTTCCGGCT TTTCCCCAGT CTCAGCATCA CCACGGAGCT CACACACCCT 
TCCAACATGC GGTTCATGCA GTTCCGTGCC AAGGACAGCT ACTCTCTGGC TCTTTCCAAA 
CTTGAAAAGC AAGAACGGGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCATTT 
GCTGCTGGTC GAGTATTTAG TATCAGCATG TTGGATACAC TGCTCTACCA GTCCTTCGTG 
AAGGACTACA TGATCACCAT CACCAGGCTG CTCTTGGGCC TTGATACTAC ACCAGGCTCC 
GGCTACCTCT GTGCAATGAA GGTAACCGAG GACGACCTGT GGATCCGCAC TTACGGCCGC 
CTCTTCCAGA AACTCTGCTC CTCCAGCGCC GAGATCCCCA TCGGCATCTA CAGGACCGAG 
TGCCATGTCT TCTCGGAGCC CCATGACGTC AGAGCCCAGG GGGGCCCAGA CATAGCCCTC 
CTGCCTGCCC AGTCTCAGAT CTCGGTGAAC ATGGAGGACT GCGAGGATAC TCGGGAGGCC 
AAGGGACCCT GGGGCACACG AGCTGCATCT GGCAGTGGCA GCACCCATGG CCGTCACGGG 
GGCAGTGCTG ACCCAGTGGA GCACCCACTA CTACGTCGCA AGAGCCTGCA GTGGGCCCGC 
AAGCTGAGTC GCAAGAGCAC CAAGCAGGCA GGGAAGGCAC CTGTGGCCAC AGACTGGATC 
ACCCAGCAGC GGCTCAGCCT GTACCGACGC TCAGAGCGCC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGAA TGAAGCACCT GGGACTGCCC ACCACTGGCT ATGAGGACGT AGCAAATTTA 
ACAGCCAGTG ATGTGATGAA TCGGGTAAAC CTGGGATATT TGCAAGATGA GATGAACGAT 
CATCACCAGA ACACCCTTTC CTATGTACTC ATCAACCCCC CGCCAGACAC AAGACTGGAA 
CCCAACGACA TTGTGTACCT CATCCGTTCC GACCCCCTGG CCCATGTGGC CAGCAGCTCC 
CAGAGTCGGA AAAGCAGCTG CAGCAACAAG CTCTCATCCT GTAATCCTGA GACCAGGGAT 
GAGACCCAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential distribution of the sodium-activated potassium channels slick and slack in mouse brain.
The Journal of comparative neurology524(10)2093-116(2016 Jul)
Rizzi S,Knaus HG,Schwarzer C

Knockout of Slo2.2 enhances itch, abolishes KNa current, and increases action potential firing frequency in DRG neurons.
eLife4(2015 Nov)
Martinez-Espinosa PL,Wu J,Yang C,Gonzalez-Perez V,Zhou H,Liang H,Xia XM,Lingle CJ

FoxP1 orchestration of ASD-relevant signaling pathways in the striatum.
Genes & development29(20)2081-96(2015 Oct)
Araujo DJ,Anderson AG,Berto S,Runnels W,Harper M,Ammanuel S,Rieger MA,Huang HC,Rajkovich K,Loerwald KW,Dekker JD,Tucker HO,Dougherty JD,Gibson JR,Konopka G

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Kcnt1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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