Twnk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Twnk
Entrez Gene ID	226153
Full Name	twinkle mtDNA helicase
Synonyms	D19Ertd626e,PEO,Peo1,Twinl
General protein information	Preferred Names twinkle mtDNA helicase Names twinkle protein, mitochondrial T7 gp4-like protein with intramitochondrial nucleoid localization progressive external ophthalmoplegia 1 protein homolog putative T7-like mitochodrial DNA helicase twinky
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 C3\|19 38.19 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006527003.3	XP_006527066.1	twinkle protein, mitochondrial isoform X1
NM_001348259.1	NP_001335188.1	twinkle protein, mitochondrial isoform 2
NM_001348254.1	NP_001335183.1	twinkle protein, mitochondrial isoform 3
NM_153796.3	NP_722491.2	twinkle protein, mitochondrial
NM_153796.4	NP_722491.2	twinkle protein, mitochondrial isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Peo1	NP_722491.2
Rattus norvegicus (Norway rat)	Peo1	NP_001101069.1
Drosophila melanogaster (fruit fly)	CG5924	NP_609318.1
Homo sapiens (human)	C10orf2	NP_068602.2
Pan troglodytes (chimpanzee)	C10H10orf2	XP_003951780.1
Canis lupus familiaris (dog)	C28H10orf2	XP_543974.1
Danio rerio (zebrafish)	peo1	NP_001264527.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010720	XP_311434.3
Caenorhabditis elegans (roundworm)	F46G11.1	NP_508973.3
Gallus gallus (chicken)	PEO1	NP_001026515.1
Xenopus tropicalis (tropical clawed frog)	LOC100491229	XP_002938766.1
	C9H10orf2	XP_001109287.1
Bos taurus (cattle)	C26H10orf2	NP_001091933.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptomic and proteomic landscape of mitochondrial dysfunction reveals secondary coenzyme Q deficiency in mammals.
K?hl I, Miranda M, Atanassov I, Kuznetsova I, Hinze Y, Mourier A, Filipovska A, Larsson NG
eLife6(2017 Nov)

Twinkle overexpression prevents cardiac rupture after myocardial infarction by alleviating impaired mitochondrial biogenesis.
Inoue T, Ikeda M, Ide T, Fujino T, Matsuo Y, Arai S, Saku K, Sunagawa K
American journal of physiology. Heart and circulatory physiology311(3)H509-19(2016 Sep)

Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.
Nikkanen J, Forsstr?m S, Euro L, Paetau I, Kohnz RA, Wang L, Chilov D, Viinam?ki J, Roivainen A, Marjam?ki P, Liljenb?ck H, Ahola S, Buzkova J, Terzioglu M, Khan NA, Pirnes-Karhu S, Paetau A, L?nnqvist T, Sajantila A, Isohanni P, Tyynismaa H, Nomura DK, Battersby BJ, Velagapudi V, Carroll CJ, Suomalainen A
Cell metabolism23(4)635-48(2016 Apr)

Regulation of DNA replication at the end of the mitochondrial D-loop involves the helicase TWINKLE and a conserved sequence element.
Jemt E, Persson ?, Shi Y, Mehmedovic M, Uhler JP, D?vila L?pez M, Freyer C, Gustafsson CM, Samuelsson T, Falkenberg M
Nucleic acids research43(19)9262-75(2015 Oct)

Overexpression of TFAM or twinkle increases mtDNA copy number and facilitates cardioprotection associated with limited mitochondrial oxidative stress.
Ikeda M, Ide T, Fujino T, Arai S, Saku K, Kakino T, Tyynismaa H, Yamasaki T, Yamada K, Kang D, Suomalainen A, Sunagawa K
PloS one10(3)e0119687(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Twinkle overexpression alleviated impaired mitochondrial biogenesis in the border area through maintained mtDNA copy number and thereby prevented cardiac rupture accompanied by the reduction of apoptosis and oxidative stress, and suppression of MMP activity.
Title: Twinkle overexpression prevents cardiac rupture after myocardial infarction by alleviating impaired mitochondrial biogenesis.

Twinkle overexpression results in increased mtDNA copy number and facilitates cardioprotection associated with limited mitochondrial oxidative stress.
Title: Overexpression of TFAM or twinkle increases mtDNA copy number and facilitates cardioprotection associated with limited mitochondrial oxidative stress.

study describes findings in a family with late onset parkinsonism and progressive external ophthalmoplegia, with mutation of G1750A in the exon 1 of PEO1 gene
Title: Twinkle mutation in an Italian family with external progressive ophthalmoplegia and parkinsonism: a case report and an update on the state of art.

TWINKLE is essential for nascent H-strand synthesis in the D-loop, thus showing that there is no separate DNA helicase responsible for replication of this region.
Title: TWINKLE is an essential mitochondrial helicase required for synthesis of nascent D-loop strands and complete mtDNA replication.

mammalian Twinkle is important for mitochondrial integrity in DA neurons and provide a novel mouse model in which increased mtDNA deletions may lead to DA neuron degeneration and parkinsonism.
Title: Mutant Twinkle increases dopaminergic neurodegeneration, mtDNA deletions and modulates Parkin expression.

The following Twnk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Twnk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu55493	NM_001348254.1 Latest version!	Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu55493	XM_006527003.3 Latest version!	Mus musculus progressive external ophthalmoplegia 1 (human) (Peo1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu83559	NM_001348259.1 Latest version!	Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu14986	NM_153796.4 Latest version!	Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$398.30 $569.00
View Old Accession Versions >>
OMu14986	NM_153796.3	Mus musculus progressive external ophthalmoplegia 1 (human) (Peo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$398.30 $569.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Twnk ( NM_001348254.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001335183.1 Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu55493
Clone ID Related Accession (Same CDS sequence)	XM_006527003.3 , NM_001348254.1
Accession Version	NM_001348254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	twinkle protein, mitochondrial isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132957.3, AK054159.1 and AV002195.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (3) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGACCC AGTTTGCTGT GACACGGCTG GAAGAGCAAC TGGACAAGTA TGAAGAGTGG 
GCAGACCGTT TTGAGGACCT GCCTCTCTAC TTCATGACTT TCCACGGACA ACAGAGCATC 
AGGTCCGTCA TAGACACAAT GCAACATGCT GTCTACGTCT ATGACGTCTG TCATGTAGTC 
ATTGACAACT TGCAGTTCAT GATGGGTCAC GAGCAGCTCT CCTCTGACAG GATTGCAGCT 
CAAGACTACA TTGTTGGAGC CTTTCGGAAG TTTGCTACAG ACAACAGTTG CCACGTGACT 
CTGGTCATTC ACCCTCGGAA AGAAGACGAC GACAAGGAAC TGCAGACAGC ATCCATTTTC 
GGCTCAGCCA AAGCAAGCCA AGAAGCAGAC AATGTTCTGA TCTTACAGGA TAGGAAACTG 
GTGACTGGGC CTGGGAAACG GTATCTGCAG GTGTCCAAGA ATCGCTTTGA TGGAGACGTA 
GGTGTCTTCC CACTGGAGTT CAACAAGAAT TCCCTTACCT TCTCCATCCC ACCCAAGAGC 
AAAGCCCGAC TCAAGAAGAT CAAGGACGAC AATGGACTAG TGGCCAAAAA ATCCTCTTCT 
GGCAAAAAAG GGGCTGCGCA CCAGAACCCT GAGATTTGCT TAGGCCAGGA CCCCAGCCCC 
GCCCAGCCAG ATACCTCCAA GTCCTCAGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335183.1
CDS	645..1337
Translation	MLTQFAVTRLEEQLDKYEEWADRFEDLPLYFMTFHGQQSIRSVIDTMQHAVYVYDVCHVVIDNLQFMMGHEQLSSDRIAAQDYIVGAFRKFATDNSCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKSKARLKKIKDDNGLVAKKSSSGKKGAAHQNPEICLGQDPSPAQPDTSKSSG

Target ORF information:

RefSeq Version	NM_001348254.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348254.1

ORF Insert Sequence:

ATGCTGACCC AGTTTGCTGT GACACGGCTG GAAGAGCAAC TGGACAAGTA TGAAGAGTGG 
GCAGACCGTT TTGAGGACCT GCCTCTCTAC TTCATGACTT TCCACGGACA ACAGAGCATC 
AGGTCCGTCA TAGACACAAT GCAACATGCT GTCTACGTCT ATGACGTCTG TCATGTAGTC 
ATTGACAACT TGCAGTTCAT GATGGGTCAC GAGCAGCTCT CCTCTGACAG GATTGCAGCT 
CAAGACTACA TTGTTGGAGC CTTTCGGAAG TTTGCTACAG ACAACAGTTG CCACGTGACT 
CTGGTCATTC ACCCTCGGAA AGAAGACGAC GACAAGGAAC TGCAGACAGC ATCCATTTTC 
GGCTCAGCCA AAGCAAGCCA AGAAGCAGAC AATGTTCTGA TCTTACAGGA TAGGAAACTG 
GTGACTGGGC CTGGGAAACG GTATCTGCAG GTGTCCAAGA ATCGCTTTGA TGGAGACGTA 
GGTGTCTTCC CACTGGAGTT CAACAAGAAT TCCCTTACCT TCTCCATCCC ACCCAAGAGC 
AAAGCCCGAC TCAAGAAGAT CAAGGACGAC AATGGACTAG TGGCCAAAAA ATCCTCTTCT 
GGCAAAAAAG GGGCTGCGCA CCAGAACCCT GAGATTTGCT TAGGCCAGGA CCCCAGCCCC 
GCCCAGCCAG ATACCTCCAA GTCCTCAGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Twnk ( XM_006527003.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527066.1 Mus musculus progressive external ophthalmoplegia 1 (human) (Peo1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55493
Clone ID Related Accession (Same CDS sequence)	XM_006527003.3 , NM_001348254.1
Accession Version	XM_006527003.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	twinkle protein, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527003.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACCC AGTTTGCTGT GACACGGCTG GAAGAGCAAC TGGACAAGTA TGAAGAGTGG 
GCAGACCGTT TTGAGGACCT GCCTCTCTAC TTCATGACTT TCCACGGACA ACAGAGCATC 
AGGTCCGTCA TAGACACAAT GCAACATGCT GTCTACGTCT ATGACGTCTG TCATGTAGTC 
ATTGACAACT TGCAGTTCAT GATGGGTCAC GAGCAGCTCT CCTCTGACAG GATTGCAGCT 
CAAGACTACA TTGTTGGAGC CTTTCGGAAG TTTGCTACAG ACAACAGTTG CCACGTGACT 
CTGGTCATTC ACCCTCGGAA AGAAGACGAC GACAAGGAAC TGCAGACAGC ATCCATTTTC 
GGCTCAGCCA AAGCAAGCCA AGAAGCAGAC AATGTTCTGA TCTTACAGGA TAGGAAACTG 
GTGACTGGGC CTGGGAAACG GTATCTGCAG GTGTCCAAGA ATCGCTTTGA TGGAGACGTA 
GGTGTCTTCC CACTGGAGTT CAACAAGAAT TCCCTTACCT TCTCCATCCC ACCCAAGAGC 
AAAGCCCGAC TCAAGAAGAT CAAGGACGAC AATGGACTAG TGGCCAAAAA ATCCTCTTCT 
GGCAAAAAAG GGGCTGCGCA CCAGAACCCT GAGATTTGCT TAGGCCAGGA CCCCAGCCCC 
GCCCAGCCAG ATACCTCCAA GTCCTCAGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527066.1
CDS	646..1338
Translation	MLTQFAVTRLEEQLDKYEEWADRFEDLPLYFMTFHGQQSIRSVIDTMQHAVYVYDVCHVVIDNLQFMMGHEQLSSDRIAAQDYIVGAFRKFATDNSCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKSKARLKKIKDDNGLVAKKSSSGKKGAAHQNPEICLGQDPSPAQPDTSKSSG

Target ORF information:

RefSeq Version	XM_006527003.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus progressive external ophthalmoplegia 1 (human) (Peo1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527003.3

ORF Insert Sequence:

ATGCTGACCC AGTTTGCTGT GACACGGCTG GAAGAGCAAC TGGACAAGTA TGAAGAGTGG 
GCAGACCGTT TTGAGGACCT GCCTCTCTAC TTCATGACTT TCCACGGACA ACAGAGCATC 
AGGTCCGTCA TAGACACAAT GCAACATGCT GTCTACGTCT ATGACGTCTG TCATGTAGTC 
ATTGACAACT TGCAGTTCAT GATGGGTCAC GAGCAGCTCT CCTCTGACAG GATTGCAGCT 
CAAGACTACA TTGTTGGAGC CTTTCGGAAG TTTGCTACAG ACAACAGTTG CCACGTGACT 
CTGGTCATTC ACCCTCGGAA AGAAGACGAC GACAAGGAAC TGCAGACAGC ATCCATTTTC 
GGCTCAGCCA AAGCAAGCCA AGAAGCAGAC AATGTTCTGA TCTTACAGGA TAGGAAACTG 
GTGACTGGGC CTGGGAAACG GTATCTGCAG GTGTCCAAGA ATCGCTTTGA TGGAGACGTA 
GGTGTCTTCC CACTGGAGTT CAACAAGAAT TCCCTTACCT TCTCCATCCC ACCCAAGAGC 
AAAGCCCGAC TCAAGAAGAT CAAGGACGAC AATGGACTAG TGGCCAAAAA ATCCTCTTCT 
GGCAAAAAAG GGGCTGCGCA CCAGAACCCT GAGATTTGCT TAGGCCAGGA CCCCAGCCCC 
GCCCAGCCAG ATACCTCCAA GTCCTCAGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Twnk ( NM_001348259.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001335188.1 Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu83559
Clone ID Related Accession (Same CDS sequence)	NM_001348259.1
Accession Version	NM_001348259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	twinkle protein, mitochondrial isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132957.3, BC034909.1 and AV002195.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGCTGCC AGACTCTCCT CTTAACTCAG AGCTTTGTCC GTCCCCCTAT AGGGCCAACA 
GGCAGTGGCA AGACAACATT CATCAGTGAG TATGCCCTGG ACTTGTGTAC CCAGGGAGTG 
AACACACTGT GGGGTAGCTT TGAGATCAGC AACGTGAGAC TAGCCCGGGT CATGCTGACC 
CAGTTTGCTG TGACACGGCT GGAAGAGCAA CTGGACAAGT ATGAAGAGTG GGCAGACCGT 
TTTGAGGACC TGCCTCTCTA CTTCATGACT TTCCACGGAC AACAGAGCAT CAGGTCCGTC 
ATAGACACAA TGCAACATGC TGTCTACGTC TATGACGTCT GTCATGTAGT CATTGACAAC 
TTGCAGTTCA TGATGGGTCA CGAGCAGCTC TCCTCTGACA GGATTGCAGC TCAAGACTAC 
ATTGTTGGAG CCTTTCGGAA GTTTGCTACA GACAACAGTT GCCACGTGAC TCTGGTCATT 
CACCCTCGGA AAGAAGACGA CGACAAGGAA CTGCAGACAG CATCCATTTT CGGCTCAGCC 
AAAGCAAGCC AAGAAGCAGA CAATGTTCTG ATCTTACAGG ATAGGAAACT GGTGACTGGG 
CCTGGGAAAC GGTATCTGCA GGTGTCCAAG AATCGCTTTG ATGGAGACGT AGGTGTCTTC 
CCACTGGAGT TCAACAAGAA TTCCCTTACC TTCTCCATCC CACCCAAGAG CAAAGCCCGA 
CTCAAGAAGA TCAAGGACGA CAATGGACTA GTGGCCAAAA AATCCTCTTC TGGCAAAAAA 
GGGGCTGCGC ACCAGAACCC TGAGATTTGC TTAGGCCAGG ACCCCAGCCC CGCCCAGCCA 
GATACCTCCA AGTCCTCAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335188.1
CDS	112..975
Translation	MCCQTLLLTQSFVRPPIGPTGSGKTTFISEYALDLCTQGVNTLWGSFEISNVRLARVMLTQFAVTRLEEQLDKYEEWADRFEDLPLYFMTFHGQQSIRSVIDTMQHAVYVYDVCHVVIDNLQFMMGHEQLSSDRIAAQDYIVGAFRKFATDNSCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKSKARLKKIKDDNGLVAKKSSSGKKGAAHQNPEICLGQDPSPAQPDTSKSSG

Target ORF information:

RefSeq Version	NM_001348259.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348259.1

ORF Insert Sequence:

ATGTGCTGCC AGACTCTCCT CTTAACTCAG AGCTTTGTCC GTCCCCCTAT AGGGCCAACA 
GGCAGTGGCA AGACAACATT CATCAGTGAG TATGCCCTGG ACTTGTGTAC CCAGGGAGTG 
AACACACTGT GGGGTAGCTT TGAGATCAGC AACGTGAGAC TAGCCCGGGT CATGCTGACC 
CAGTTTGCTG TGACACGGCT GGAAGAGCAA CTGGACAAGT ATGAAGAGTG GGCAGACCGT 
TTTGAGGACC TGCCTCTCTA CTTCATGACT TTCCACGGAC AACAGAGCAT CAGGTCCGTC 
ATAGACACAA TGCAACATGC TGTCTACGTC TATGACGTCT GTCATGTAGT CATTGACAAC 
TTGCAGTTCA TGATGGGTCA CGAGCAGCTC TCCTCTGACA GGATTGCAGC TCAAGACTAC 
ATTGTTGGAG CCTTTCGGAA GTTTGCTACA GACAACAGTT GCCACGTGAC TCTGGTCATT 
CACCCTCGGA AAGAAGACGA CGACAAGGAA CTGCAGACAG CATCCATTTT CGGCTCAGCC 
AAAGCAAGCC AAGAAGCAGA CAATGTTCTG ATCTTACAGG ATAGGAAACT GGTGACTGGG 
CCTGGGAAAC GGTATCTGCA GGTGTCCAAG AATCGCTTTG ATGGAGACGT AGGTGTCTTC 
CCACTGGAGT TCAACAAGAA TTCCCTTACC TTCTCCATCC CACCCAAGAG CAAAGCCCGA 
CTCAAGAAGA TCAAGGACGA CAATGGACTA GTGGCCAAAA AATCCTCTTC TGGCAAAAAA 
GGGGCTGCGC ACCAGAACCC TGAGATTTGC TTAGGCCAGG ACCCCAGCCC CGCCCAGCCA 
GATACCTCCA AGTCCTCAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Twnk ( NM_153796.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_722491.2 Mus musculus progressive external ophthalmoplegia 1 (human) (Peo1), mRNA.

Price & Availability↑

CloneID	OMu14986
Clone ID Related Accession (Same CDS sequence)	NM_153796.3 , NM_153796.4
Accession Version	NM_153796.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-04
Organism	Mus musculus(House mouse)
Product	twinkle protein, mitochondrial
Comment

ATGTGGCTTC TTCTCCGACG TGCATATCCC CTCCGGATCT TGCTGCCACT GCGTGGCGAG 
TGGGTGGGTA GGAGGGGCCT GCCCAGAAGC TTGGCGCCAG GCCCTCCCCG CAGAAGGTAC 
AGAAAAGAAG CGCTCCCAGC CTTAGAGATG CCAGTGTCGC CGGTGACTAC GACTGAAATC 
CGCCAGTATT TACGTGCACA TGGGATCCCC TTCCAGGATG GCCACAGCTG CCTTCGAGCA 
CCAAGTCCCT TTGTGGTGTC CTCAGACATC AAAAATGAGA AAAAGGACGC TCCCACTTCC 
TTCTGCCTCT TCATTGACAA GACCACAGGA CACTTTCTTT GCATGACCAG CCTAGCAGAA 
GGGAGCTGGG AAGACTTGCA GGCCAGTGTG GAGGGACGAG GGGATGGGGC CAAAGAGGGT 
GTCCTACTTA GGGAAGGCCC AGAAGCTGAG GTTAGGGAGG AGGTCTTGAG GATCTGGAAC 
CGAGCCATAC CTCTTTGGGA GCTTCCAGAC CCTGAGGAAG CCCAGCTAGC TCGTGTGATG 
TTTGGCCTTA CCAAGGTGAC AGATGACACA CTCAGGCGAT TCAGTGTCCG CTATCTTCGA 
TCTGCTCGAA GTCTGGTCTT CCCTTGGTTC ACCCCTGGGA GCTCAGGATT ACGTGGCCTG 
AAGCTACTAG GGGCTGAGGG TCAGGAAAAT GGAGTACAGT ATGTAGAGAC CACCATCCCC 
CGGCCTGGTG TCTATCACAA CCTATTTGGA TTACCACTGA TCAGCCGGAG AGATACTGAG 
GTGGTTGTGA CAAGTCGTGA ACTGGACAGC CTGGCCTTGA GCCAGTCGAC GGGGCTGCCA 
ACCCTTTCCC TACCCCGAGG GACAGTCTGC TTACCCCCAG CCTTACTGCC TTACCTTGAA 
CAGTTCCGTC GGATTGTGTT CTGGTTAGGA GATGACCTTC GATCCTGGGA AGCTGCCAAA 
CTGTTTGCTC GAAAACTCAA CCCCAAGCGA TGCTCTCTGG TGAGGCCTGG GAACCAACAG 
CCTCGTCCCC TGGAGGCCTT AAACCAAGGC TTAAGTCTTC CCCGTATTCT TCGTACCGCC 
CTACCTGCCT GGCACAAGTC TATTGTGTCT TTTCGGCAGC TCCGAGAGGA GGTTTTAGGA 
GAACTGTCAA ATGTAGAGCA AGCGGCTGGT GTCCGTTGGA GCCGCTTCCC AGACCTGAAT 
CGTCTGCTGA AGGGACATCG GAAGGGCGAG TTGACGGTCT TTACAGGGCC AACAGGCAGT 
GGCAAGACAA CATTCATCAG TGAGTATGCC CTGGACTTGT GTACCCAGGG AGTGAACACA 
CTGTGGGGTA GCTTTGAGAT CAGCAACGTG AGACTAGCCC GGGTCATGCT GACCCAGTTT 
GCTGTGACAC GGCTGGAAGA GCAACTGGAC AAGTATGAAG AGTGGGCAGA CCGTTTTGAG 
GACCTGCCTC TCTACTTCAT GACTTTCCAC GGACAACAGA GCATCAGGTC CGTCATAGAC 
ACAATGCAAC ATGCTGTCTA CGTCTATGAC GTCTGTCATG TAGTCATTGA CAACTTGCAG 
TTCATGATGG GTCACGAGCA GCTCTCCTCT GACAGGATTG CAGCTCAAGA CTACATTGTT 
GGAGCCTTTC GGAAGTTTGC TACAGACAAC AGTTGCCACG TGACTCTGGT CATTCACCCT 
CGGAAAGAAG ACGACGACAA GGAACTGCAG ACAGCATCCA TTTTCGGCTC AGCCAAAGCA 
AGCCAAGAAG CAGACAATGT TCTGATCTTA CAGGATAGGA AACTGGTGAC TGGGCCTGGG 
AAACGGTATC TGCAGGTGTC CAAGAATCGC TTTGATGGAG ACGTAGGTGT CTTCCCACTG 
GAGTTCAACA AGAATTCCCT TACCTTCTCC ATCCCACCCA AGAGCAAAGC CCGACTCAAG 
AAGATCAAGG ACGACAATGG ACTAGTGGCC AAAAAATCCT CTTCTGGCAA AAAAGGGGCT 
GCGCACCAGA ACCCTGAGAT TTGCTTAGGC CAGGACCCCA GCCCCGCCCA GCCAGATACC 
TCCAAGTCCT CAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_722491.2

Translation

MWLLLRRAYPLRILLPLRGEWVGRRGLPRSLAPGPPRRRYRKEALPALEMPVSPVTTTEIRQYLRAHGIPFQDGHSCLRAPSPFVVSSDIKNEKKDAPTSFCLFIDKTTGHFLCMTSLAEGSWEDLQASVEGRGDGAKEGVLLREGPEAEVREEVLRIWNRAIPLWELPDPEEAQLARVMFGLTKVTDDTLRRFSVRYLRSARSLVFPWFTPGSSGLRGLKLLGAEGQENGVQYVETTIPRPGVYHNLFGLPLISRRDTEVVVTSRELDSLALSQSTGLPTLSLPRGTVCLPPALLPYLEQFRRIVFWLGDDLRSWEAAKLFARKLNPKRCSLVRPGNQQPRPLEALNQGLSLPRILRTALPAWHKSIVSFRQLREEVLGELSNVEQAAGVRWSRFPDLNRLLKGHRKGELTVFTGPTGSGKTTFISEYALDLCTQGVNTLWGSFEISNVRLARVMLTQFAVTRLEEQLDKYEEWADRFEDLPLYFMTFHGQQSIRSVIDTMQHAVYVYDVCHVVIDNLQFMMGHEQLSSDRIAAQDYIVGAFRKFATDNSCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKSKARLKKIKDDNGLVAKKSSSGKKGAAHQNPEICLGQDPSPAQPDTSKSSG

Target ORF information:

RefSeq Version	NM_153796.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus progressive external ophthalmoplegia 1 (human) (Peo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153796.3

ORF Insert Sequence:

ATGTGGCTTC TTCTCCGACG TGCATATCCC CTCCGGATCT TGCTGCCACT GCGTGGCGAG 
TGGGTGGGTA GGAGGGGCCT GCCCAGAAGC TTGGCGCCAG GCCCTCCCCG CAGAAGGTAC 
AGAAAAGAAG CGCTCCCAGC CTTAGAGATG CCAGTGTCGC CGGTGACTAC GACTGAAATC 
CGCCAGTATT TACGTGCACA TGGGATCCCC TTCCAGGATG GCCACAGCTG CCTTCGAGCA 
CCAAGTCCCT TTGTGGTGTC CTCAGACATC AAAAATGAGA AAAAGGACGC TCCCACTTCC 
TTCTGCCTCT TCATTGACAA GACCACAGGA CACTTTCTTT GCATGACCAG CCTAGCAGAA 
GGGAGCTGGG AAGACTTGCA GGCCAGTGTG GAGGGACGAG GGGATGGGGC CAAAGAGGGT 
GTCCTACTTA GGGAAGGCCC AGAAGCTGAG GTTAGGGAGG AGGTCTTGAG GATCTGGAAC 
CGAGCCATAC CTCTTTGGGA GCTTCCAGAC CCTGAGGAAG CCCAGCTAGC TCGTGTGATG 
TTTGGCCTTA CCAAGGTGAC AGATGACACA CTCAGGCGAT TCAGTGTCCG CTATCTTCGA 
TCTGCTCGAA GTCTGGTCTT CCCTTGGTTC ACCCCTGGGA GCTCAGGATT ACGTGGCCTG 
AAGCTACTAG GGGCTGAGGG TCAGGAAAAT GGAGTACAGT ATGTAGAGAC CACCATCCCC 
CGGCCTGGTG TCTATCACAA CCTATTTGGA TTACCACTGA TCAGCCGGAG AGATACTGAG 
GTGGTTGTGA CAAGTCGTGA ACTGGACAGC CTGGCCTTGA GCCAGTCGAC GGGGCTGCCA 
ACCCTTTCCC TACCCCGAGG GACAGTCTGC TTACCCCCAG CCTTACTGCC TTACCTTGAA 
CAGTTCCGTC GGATTGTGTT CTGGTTAGGA GATGACCTTC GATCCTGGGA AGCTGCCAAA 
CTGTTTGCTC GAAAACTCAA CCCCAAGCGA TGCTCTCTGG TGAGGCCTGG GAACCAACAG 
CCTCGTCCCC TGGAGGCCTT AAACCAAGGC TTAAGTCTTC CCCGTATTCT TCGTACCGCC 
CTACCTGCCT GGCACAAGTC TATTGTGTCT TTTCGGCAGC TCCGAGAGGA GGTTTTAGGA 
GAACTGTCAA ATGTAGAGCA AGCGGCTGGT GTCCGTTGGA GCCGCTTCCC AGACCTGAAT 
CGTCTGCTGA AGGGACATCG GAAGGGCGAG TTGACGGTCT TTACAGGGCC AACAGGCAGT 
GGCAAGACAA CATTCATCAG TGAGTATGCC CTGGACTTGT GTACCCAGGG AGTGAACACA 
CTGTGGGGTA GCTTTGAGAT CAGCAACGTG AGACTAGCCC GGGTCATGCT GACCCAGTTT 
GCTGTGACAC GGCTGGAAGA GCAACTGGAC AAGTATGAAG AGTGGGCAGA CCGTTTTGAG 
GACCTGCCTC TCTACTTCAT GACTTTCCAC GGACAACAGA GCATCAGGTC CGTCATAGAC 
ACAATGCAAC ATGCTGTCTA CGTCTATGAC GTCTGTCATG TAGTCATTGA CAACTTGCAG 
TTCATGATGG GTCACGAGCA GCTCTCCTCT GACAGGATTG CAGCTCAAGA CTACATTGTT 
GGAGCCTTTC GGAAGTTTGC TACAGACAAC AGTTGCCACG TGACTCTGGT CATTCACCCT 
CGGAAAGAAG ACGACGACAA GGAACTGCAG ACAGCATCCA TTTTCGGCTC AGCCAAAGCA 
AGCCAAGAAG CAGACAATGT TCTGATCTTA CAGGATAGGA AACTGGTGAC TGGGCCTGGG 
AAACGGTATC TGCAGGTGTC CAAGAATCGC TTTGATGGAG ACGTAGGTGT CTTCCCACTG 
GAGTTCAACA AGAATTCCCT TACCTTCTCC ATCCCACCCA AGAGCAAAGC CCGACTCAAG 
AAGATCAAGG ACGACAATGG ACTAGTGGCC AAAAAATCCT CTTCTGGCAA AAAAGGGGCT 
GCGCACCAGA ACCCTGAGAT TTGCTTAGGC CAGGACCCCA GCCCCGCCCA GCCAGATACC 
TCCAAGTCCT CAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Twnk ( NM_153796.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_722491.2 Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 1, mRNA.

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CloneID	OMu14986
Clone ID Related Accession (Same CDS sequence)	NM_153796.3 , NM_153796.4
Accession Version	NM_153796.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	twinkle protein, mitochondrial isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132957.3, CA534850.1, BF225236.1, CA537258.1, AK054159.1 and AV002195.1. On Feb 8, 2017 this sequence version replaced NM_153796.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGCTTC TTCTCCGACG TGCATATCCC CTCCGGATCT TGCTGCCACT GCGTGGCGAG 
TGGGTGGGTA GGAGGGGCCT GCCCAGAAGC TTGGCGCCAG GCCCTCCCCG CAGAAGGTAC 
AGAAAAGAAG CGCTCCCAGC CTTAGAGATG CCAGTGTCGC CGGTGACTAC GACTGAAATC 
CGCCAGTATT TACGTGCACA TGGGATCCCC TTCCAGGATG GCCACAGCTG CCTTCGAGCA 
CCAAGTCCCT TTGTGGTGTC CTCAGACATC AAAAATGAGA AAAAGGACGC TCCCACTTCC 
TTCTGCCTCT TCATTGACAA GACCACAGGA CACTTTCTTT GCATGACCAG CCTAGCAGAA 
GGGAGCTGGG AAGACTTGCA GGCCAGTGTG GAGGGACGAG GGGATGGGGC CAAAGAGGGT 
GTCCTACTTA GGGAAGGCCC AGAAGCTGAG GTTAGGGAGG AGGTCTTGAG GATCTGGAAC 
CGAGCCATAC CTCTTTGGGA GCTTCCAGAC CCTGAGGAAG CCCAGCTAGC TCGTGTGATG 
TTTGGCCTTA CCAAGGTGAC AGATGACACA CTCAGGCGAT TCAGTGTCCG CTATCTTCGA 
TCTGCTCGAA GTCTGGTCTT CCCTTGGTTC ACCCCTGGGA GCTCAGGATT ACGTGGCCTG 
AAGCTACTAG GGGCTGAGGG TCAGGAAAAT GGAGTACAGT ATGTAGAGAC CACCATCCCC 
CGGCCTGGTG TCTATCACAA CCTATTTGGA TTACCACTGA TCAGCCGGAG AGATACTGAG 
GTGGTTGTGA CAAGTCGTGA ACTGGACAGC CTGGCCTTGA GCCAGTCGAC GGGGCTGCCA 
ACCCTTTCCC TACCCCGAGG GACAGTCTGC TTACCCCCAG CCTTACTGCC TTACCTTGAA 
CAGTTCCGTC GGATTGTGTT CTGGTTAGGA GATGACCTTC GATCCTGGGA AGCTGCCAAA 
CTGTTTGCTC GAAAACTCAA CCCCAAGCGA TGCTCTCTGG TGAGGCCTGG GAACCAACAG 
CCTCGTCCCC TGGAGGCCTT AAACCAAGGC TTAAGTCTTC CCCGTATTCT TCGTACCGCC 
CTACCTGCCT GGCACAAGTC TATTGTGTCT TTTCGGCAGC TCCGAGAGGA GGTTTTAGGA 
GAACTGTCAA ATGTAGAGCA AGCGGCTGGT GTCCGTTGGA GCCGCTTCCC AGACCTGAAT 
CGTCTGCTGA AGGGACATCG GAAGGGCGAG TTGACGGTCT TTACAGGGCC AACAGGCAGT 
GGCAAGACAA CATTCATCAG TGAGTATGCC CTGGACTTGT GTACCCAGGG AGTGAACACA 
CTGTGGGGTA GCTTTGAGAT CAGCAACGTG AGACTAGCCC GGGTCATGCT GACCCAGTTT 
GCTGTGACAC GGCTGGAAGA GCAACTGGAC AAGTATGAAG AGTGGGCAGA CCGTTTTGAG 
GACCTGCCTC TCTACTTCAT GACTTTCCAC GGACAACAGA GCATCAGGTC CGTCATAGAC 
ACAATGCAAC ATGCTGTCTA CGTCTATGAC GTCTGTCATG TAGTCATTGA CAACTTGCAG 
TTCATGATGG GTCACGAGCA GCTCTCCTCT GACAGGATTG CAGCTCAAGA CTACATTGTT 
GGAGCCTTTC GGAAGTTTGC TACAGACAAC AGTTGCCACG TGACTCTGGT CATTCACCCT 
CGGAAAGAAG ACGACGACAA GGAACTGCAG ACAGCATCCA TTTTCGGCTC AGCCAAAGCA 
AGCCAAGAAG CAGACAATGT TCTGATCTTA CAGGATAGGA AACTGGTGAC TGGGCCTGGG 
AAACGGTATC TGCAGGTGTC CAAGAATCGC TTTGATGGAG ACGTAGGTGT CTTCCCACTG 
GAGTTCAACA AGAATTCCCT TACCTTCTCC ATCCCACCCA AGAGCAAAGC CCGACTCAAG 
AAGATCAAGG ACGACAATGG ACTAGTGGCC AAAAAATCCT CTTCTGGCAA AAAAGGGGCT 
GCGCACCAGA ACCCTGAGAT TTGCTTAGGC CAGGACCCCA GCCCCGCCCA GCCAGATACC 
TCCAAGTCCT CAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722491.2
CDS	573..2630
Translation	MWLLLRRAYPLRILLPLRGEWVGRRGLPRSLAPGPPRRRYRKEALPALEMPVSPVTTTEIRQYLRAHGIPFQDGHSCLRAPSPFVVSSDIKNEKKDAPTSFCLFIDKTTGHFLCMTSLAEGSWEDLQASVEGRGDGAKEGVLLREGPEAEVREEVLRIWNRAIPLWELPDPEEAQLARVMFGLTKVTDDTLRRFSVRYLRSARSLVFPWFTPGSSGLRGLKLLGAEGQENGVQYVETTIPRPGVYHNLFGLPLISRRDTEVVVTSRELDSLALSQSTGLPTLSLPRGTVCLPPALLPYLEQFRRIVFWLGDDLRSWEAAKLFARKLNPKRCSLVRPGNQQPRPLEALNQGLSLPRILRTALPAWHKSIVSFRQLREEVLGELSNVEQAAGVRWSRFPDLNRLLKGHRKGELTVFTGPTGSGKTTFISEYALDLCTQGVNTLWGSFEISNVRLARVMLTQFAVTRLEEQLDKYEEWADRFEDLPLYFMTFHGQQSIRSVIDTMQHAVYVYDVCHVVIDNLQFMMGHEQLSSDRIAAQDYIVGAFRKFATDNSCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKSKARLKKIKDDNGLVAKKSSSGKKGAAHQNPEICLGQDPSPAQPDTSKSSG

Target ORF information:

RefSeq Version	NM_153796.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153796.4

ORF Insert Sequence:

ATGTGGCTTC TTCTCCGACG TGCATATCCC CTCCGGATCT TGCTGCCACT GCGTGGCGAG 
TGGGTGGGTA GGAGGGGCCT GCCCAGAAGC TTGGCGCCAG GCCCTCCCCG CAGAAGGTAC 
AGAAAAGAAG CGCTCCCAGC CTTAGAGATG CCAGTGTCGC CGGTGACTAC GACTGAAATC 
CGCCAGTATT TACGTGCACA TGGGATCCCC TTCCAGGATG GCCACAGCTG CCTTCGAGCA 
CCAAGTCCCT TTGTGGTGTC CTCAGACATC AAAAATGAGA AAAAGGACGC TCCCACTTCC 
TTCTGCCTCT TCATTGACAA GACCACAGGA CACTTTCTTT GCATGACCAG CCTAGCAGAA 
GGGAGCTGGG AAGACTTGCA GGCCAGTGTG GAGGGACGAG GGGATGGGGC CAAAGAGGGT 
GTCCTACTTA GGGAAGGCCC AGAAGCTGAG GTTAGGGAGG AGGTCTTGAG GATCTGGAAC 
CGAGCCATAC CTCTTTGGGA GCTTCCAGAC CCTGAGGAAG CCCAGCTAGC TCGTGTGATG 
TTTGGCCTTA CCAAGGTGAC AGATGACACA CTCAGGCGAT TCAGTGTCCG CTATCTTCGA 
TCTGCTCGAA GTCTGGTCTT CCCTTGGTTC ACCCCTGGGA GCTCAGGATT ACGTGGCCTG 
AAGCTACTAG GGGCTGAGGG TCAGGAAAAT GGAGTACAGT ATGTAGAGAC CACCATCCCC 
CGGCCTGGTG TCTATCACAA CCTATTTGGA TTACCACTGA TCAGCCGGAG AGATACTGAG 
GTGGTTGTGA CAAGTCGTGA ACTGGACAGC CTGGCCTTGA GCCAGTCGAC GGGGCTGCCA 
ACCCTTTCCC TACCCCGAGG GACAGTCTGC TTACCCCCAG CCTTACTGCC TTACCTTGAA 
CAGTTCCGTC GGATTGTGTT CTGGTTAGGA GATGACCTTC GATCCTGGGA AGCTGCCAAA 
CTGTTTGCTC GAAAACTCAA CCCCAAGCGA TGCTCTCTGG TGAGGCCTGG GAACCAACAG 
CCTCGTCCCC TGGAGGCCTT AAACCAAGGC TTAAGTCTTC CCCGTATTCT TCGTACCGCC 
CTACCTGCCT GGCACAAGTC TATTGTGTCT TTTCGGCAGC TCCGAGAGGA GGTTTTAGGA 
GAACTGTCAA ATGTAGAGCA AGCGGCTGGT GTCCGTTGGA GCCGCTTCCC AGACCTGAAT 
CGTCTGCTGA AGGGACATCG GAAGGGCGAG TTGACGGTCT TTACAGGGCC AACAGGCAGT 
GGCAAGACAA CATTCATCAG TGAGTATGCC CTGGACTTGT GTACCCAGGG AGTGAACACA 
CTGTGGGGTA GCTTTGAGAT CAGCAACGTG AGACTAGCCC GGGTCATGCT GACCCAGTTT 
GCTGTGACAC GGCTGGAAGA GCAACTGGAC AAGTATGAAG AGTGGGCAGA CCGTTTTGAG 
GACCTGCCTC TCTACTTCAT GACTTTCCAC GGACAACAGA GCATCAGGTC CGTCATAGAC 
ACAATGCAAC ATGCTGTCTA CGTCTATGAC GTCTGTCATG TAGTCATTGA CAACTTGCAG 
TTCATGATGG GTCACGAGCA GCTCTCCTCT GACAGGATTG CAGCTCAAGA CTACATTGTT 
GGAGCCTTTC GGAAGTTTGC TACAGACAAC AGTTGCCACG TGACTCTGGT CATTCACCCT 
CGGAAAGAAG ACGACGACAA GGAACTGCAG ACAGCATCCA TTTTCGGCTC AGCCAAAGCA 
AGCCAAGAAG CAGACAATGT TCTGATCTTA CAGGATAGGA AACTGGTGAC TGGGCCTGGG 
AAACGGTATC TGCAGGTGTC CAAGAATCGC TTTGATGGAG ACGTAGGTGT CTTCCCACTG 
GAGTTCAACA AGAATTCCCT TACCTTCTCC ATCCCACCCA AGAGCAAAGC CCGACTCAAG 
AAGATCAAGG ACGACAATGG ACTAGTGGCC AAAAAATCCT CTTCTGGCAA AAAAGGGGCT 
GCGCACCAGA ACCCTGAGAT TTGCTTAGGC CAGGACCCCA GCCCCGCCCA GCCAGATACC 
TCCAAGTCCT CAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptomic and proteomic landscape of mitochondrial dysfunction reveals secondary coenzyme Q deficiency in mammals.
eLife6(2017 Nov)
K?hl I,Miranda M,Atanassov I,Kuznetsova I,Hinze Y,Mourier A,Filipovska A,Larsson NG

Twinkle overexpression prevents cardiac rupture after myocardial infarction by alleviating impaired mitochondrial biogenesis.
American journal of physiology. Heart and circulatory physiology311(3)H509-19(2016 Sep)
Inoue T,Ikeda M,Ide T,Fujino T,Matsuo Y,Arai S,Saku K,Sunagawa K

Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.
Cell metabolism23(4)635-48(2016 Apr)
Nikkanen J,Forsstr?m S,Euro L,Paetau I,Kohnz RA,Wang L,Chilov D,Viinam?ki J,Roivainen A,Marjam?ki P,Liljenb?ck H,Ahola S,Buzkova J,Terzioglu M,Khan NA,Pirnes-Karhu S,Paetau A,L?nnqvist T,Sajantila A,Isohanni P,Tyynismaa H,Nomura DK,Battersby BJ,Velagapudi V,Carroll CJ,Suomalainen A

Regulation of DNA replication at the end of the mitochondrial D-loop involves the helicase TWINKLE and a conserved sequence element.
Nucleic acids research43(19)9262-75(2015 Oct)
Jemt E,Persson ?,Shi Y,Mehmedovic M,Uhler JP,D?vila L?pez M,Freyer C,Gustafsson CM,Samuelsson T,Falkenberg M

Overexpression of TFAM or twinkle increases mtDNA copy number and facilitates cardioprotection associated with limited mitochondrial oxidative stress.
PloS one10(3)e0119687(2015)
Ikeda M,Ide T,Fujino T,Arai S,Saku K,Kakino T,Tyynismaa H,Yamasaki T,Yamada K,Kang D,Suomalainen A,Sunagawa K

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Twnk cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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