Twnk cDNA ORF clone, Mus musculus(house mouse)

The following Twnk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Twnk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu55493 NM_001348254.1
Latest version!
Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OMu55493 XM_006527003.3
Latest version!
Mus musculus progressive external ophthalmoplegia 1 (human) (Peo1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OMu83559 NM_001348259.1
Latest version!
Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OMu14986 NM_153796.4
Latest version!
Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $398.30
$569.00
View Old Accession Versions >>
OMu14986 NM_153796.3 Mus musculus progressive external ophthalmoplegia 1 (human) (Peo1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $398.30
$569.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu55493
    Clone ID Related Accession (Same CDS sequence) XM_006527003.3 , NM_001348254.1
    Accession Version NM_001348254.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-24
    Organism Mus musculus(house mouse)
    Product twinkle protein, mitochondrial isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132957.3, AK054159.1 and AV002195.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (3) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACCC AGTTTGCTGT GACACGGCTG GAAGAGCAAC TGGACAAGTA TGAAGAGTGG 
    GCAGACCGTT TTGAGGACCT GCCTCTCTAC TTCATGACTT TCCACGGACA ACAGAGCATC
    AGGTCCGTCA TAGACACAAT GCAACATGCT GTCTACGTCT ATGACGTCTG TCATGTAGTC
    ATTGACAACT TGCAGTTCAT GATGGGTCAC GAGCAGCTCT CCTCTGACAG GATTGCAGCT
    CAAGACTACA TTGTTGGAGC CTTTCGGAAG TTTGCTACAG ACAACAGTTG CCACGTGACT
    CTGGTCATTC ACCCTCGGAA AGAAGACGAC GACAAGGAAC TGCAGACAGC ATCCATTTTC
    GGCTCAGCCA AAGCAAGCCA AGAAGCAGAC AATGTTCTGA TCTTACAGGA TAGGAAACTG
    GTGACTGGGC CTGGGAAACG GTATCTGCAG GTGTCCAAGA ATCGCTTTGA TGGAGACGTA
    GGTGTCTTCC CACTGGAGTT CAACAAGAAT TCCCTTACCT TCTCCATCCC ACCCAAGAGC
    AAAGCCCGAC TCAAGAAGAT CAAGGACGAC AATGGACTAG TGGCCAAAAA ATCCTCTTCT
    GGCAAAAAAG GGGCTGCGCA CCAGAACCCT GAGATTTGCT TAGGCCAGGA CCCCAGCCCC
    GCCCAGCCAG ATACCTCCAA GTCCTCAGGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001335183.1
    CDS645..1337
    Translation

    Target ORF information:

    RefSeq Version NM_001348254.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001348254.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACCC AGTTTGCTGT GACACGGCTG GAAGAGCAAC TGGACAAGTA TGAAGAGTGG 
    GCAGACCGTT TTGAGGACCT GCCTCTCTAC TTCATGACTT TCCACGGACA ACAGAGCATC
    AGGTCCGTCA TAGACACAAT GCAACATGCT GTCTACGTCT ATGACGTCTG TCATGTAGTC
    ATTGACAACT TGCAGTTCAT GATGGGTCAC GAGCAGCTCT CCTCTGACAG GATTGCAGCT
    CAAGACTACA TTGTTGGAGC CTTTCGGAAG TTTGCTACAG ACAACAGTTG CCACGTGACT
    CTGGTCATTC ACCCTCGGAA AGAAGACGAC GACAAGGAAC TGCAGACAGC ATCCATTTTC
    GGCTCAGCCA AAGCAAGCCA AGAAGCAGAC AATGTTCTGA TCTTACAGGA TAGGAAACTG
    GTGACTGGGC CTGGGAAACG GTATCTGCAG GTGTCCAAGA ATCGCTTTGA TGGAGACGTA
    GGTGTCTTCC CACTGGAGTT CAACAAGAAT TCCCTTACCT TCTCCATCCC ACCCAAGAGC
    AAAGCCCGAC TCAAGAAGAT CAAGGACGAC AATGGACTAG TGGCCAAAAA ATCCTCTTCT
    GGCAAAAAAG GGGCTGCGCA CCAGAACCCT GAGATTTGCT TAGGCCAGGA CCCCAGCCCC
    GCCCAGCCAG ATACCTCCAA GTCCTCAGGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu55493
    Clone ID Related Accession (Same CDS sequence) XM_006527003.3 , NM_001348254.1
    Accession Version XM_006527003.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product twinkle protein, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527003.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACCC AGTTTGCTGT GACACGGCTG GAAGAGCAAC TGGACAAGTA TGAAGAGTGG 
    GCAGACCGTT TTGAGGACCT GCCTCTCTAC TTCATGACTT TCCACGGACA ACAGAGCATC
    AGGTCCGTCA TAGACACAAT GCAACATGCT GTCTACGTCT ATGACGTCTG TCATGTAGTC
    ATTGACAACT TGCAGTTCAT GATGGGTCAC GAGCAGCTCT CCTCTGACAG GATTGCAGCT
    CAAGACTACA TTGTTGGAGC CTTTCGGAAG TTTGCTACAG ACAACAGTTG CCACGTGACT
    CTGGTCATTC ACCCTCGGAA AGAAGACGAC GACAAGGAAC TGCAGACAGC ATCCATTTTC
    GGCTCAGCCA AAGCAAGCCA AGAAGCAGAC AATGTTCTGA TCTTACAGGA TAGGAAACTG
    GTGACTGGGC CTGGGAAACG GTATCTGCAG GTGTCCAAGA ATCGCTTTGA TGGAGACGTA
    GGTGTCTTCC CACTGGAGTT CAACAAGAAT TCCCTTACCT TCTCCATCCC ACCCAAGAGC
    AAAGCCCGAC TCAAGAAGAT CAAGGACGAC AATGGACTAG TGGCCAAAAA ATCCTCTTCT
    GGCAAAAAAG GGGCTGCGCA CCAGAACCCT GAGATTTGCT TAGGCCAGGA CCCCAGCCCC
    GCCCAGCCAG ATACCTCCAA GTCCTCAGGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527066.1
    CDS646..1338
    Translation

    Target ORF information:

    RefSeq Version XM_006527003.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus progressive external ophthalmoplegia 1 (human) (Peo1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527003.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACCC AGTTTGCTGT GACACGGCTG GAAGAGCAAC TGGACAAGTA TGAAGAGTGG 
    GCAGACCGTT TTGAGGACCT GCCTCTCTAC TTCATGACTT TCCACGGACA ACAGAGCATC
    AGGTCCGTCA TAGACACAAT GCAACATGCT GTCTACGTCT ATGACGTCTG TCATGTAGTC
    ATTGACAACT TGCAGTTCAT GATGGGTCAC GAGCAGCTCT CCTCTGACAG GATTGCAGCT
    CAAGACTACA TTGTTGGAGC CTTTCGGAAG TTTGCTACAG ACAACAGTTG CCACGTGACT
    CTGGTCATTC ACCCTCGGAA AGAAGACGAC GACAAGGAAC TGCAGACAGC ATCCATTTTC
    GGCTCAGCCA AAGCAAGCCA AGAAGCAGAC AATGTTCTGA TCTTACAGGA TAGGAAACTG
    GTGACTGGGC CTGGGAAACG GTATCTGCAG GTGTCCAAGA ATCGCTTTGA TGGAGACGTA
    GGTGTCTTCC CACTGGAGTT CAACAAGAAT TCCCTTACCT TCTCCATCCC ACCCAAGAGC
    AAAGCCCGAC TCAAGAAGAT CAAGGACGAC AATGGACTAG TGGCCAAAAA ATCCTCTTCT
    GGCAAAAAAG GGGCTGCGCA CCAGAACCCT GAGATTTGCT TAGGCCAGGA CCCCAGCCCC
    GCCCAGCCAG ATACCTCCAA GTCCTCAGGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu83559
    Clone ID Related Accession (Same CDS sequence) NM_001348259.1
    Accession Version NM_001348259.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-09
    Organism Mus musculus(house mouse)
    Product twinkle protein, mitochondrial isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132957.3, BC034909.1 and AV002195.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTGCTGCC AGACTCTCCT CTTAACTCAG AGCTTTGTCC GTCCCCCTAT AGGGCCAACA 
    GGCAGTGGCA AGACAACATT CATCAGTGAG TATGCCCTGG ACTTGTGTAC CCAGGGAGTG
    AACACACTGT GGGGTAGCTT TGAGATCAGC AACGTGAGAC TAGCCCGGGT CATGCTGACC
    CAGTTTGCTG TGACACGGCT GGAAGAGCAA CTGGACAAGT ATGAAGAGTG GGCAGACCGT
    TTTGAGGACC TGCCTCTCTA CTTCATGACT TTCCACGGAC AACAGAGCAT CAGGTCCGTC
    ATAGACACAA TGCAACATGC TGTCTACGTC TATGACGTCT GTCATGTAGT CATTGACAAC
    TTGCAGTTCA TGATGGGTCA CGAGCAGCTC TCCTCTGACA GGATTGCAGC TCAAGACTAC
    ATTGTTGGAG CCTTTCGGAA GTTTGCTACA GACAACAGTT GCCACGTGAC TCTGGTCATT
    CACCCTCGGA AAGAAGACGA CGACAAGGAA CTGCAGACAG CATCCATTTT CGGCTCAGCC
    AAAGCAAGCC AAGAAGCAGA CAATGTTCTG ATCTTACAGG ATAGGAAACT GGTGACTGGG
    CCTGGGAAAC GGTATCTGCA GGTGTCCAAG AATCGCTTTG ATGGAGACGT AGGTGTCTTC
    CCACTGGAGT TCAACAAGAA TTCCCTTACC TTCTCCATCC CACCCAAGAG CAAAGCCCGA
    CTCAAGAAGA TCAAGGACGA CAATGGACTA GTGGCCAAAA AATCCTCTTC TGGCAAAAAA
    GGGGCTGCGC ACCAGAACCC TGAGATTTGC TTAGGCCAGG ACCCCAGCCC CGCCCAGCCA
    GATACCTCCA AGTCCTCAGG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001335188.1
    CDS112..975
    Translation

    Target ORF information:

    RefSeq Version NM_001348259.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001348259.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTGCTGCC AGACTCTCCT CTTAACTCAG AGCTTTGTCC GTCCCCCTAT AGGGCCAACA 
    GGCAGTGGCA AGACAACATT CATCAGTGAG TATGCCCTGG ACTTGTGTAC CCAGGGAGTG
    AACACACTGT GGGGTAGCTT TGAGATCAGC AACGTGAGAC TAGCCCGGGT CATGCTGACC
    CAGTTTGCTG TGACACGGCT GGAAGAGCAA CTGGACAAGT ATGAAGAGTG GGCAGACCGT
    TTTGAGGACC TGCCTCTCTA CTTCATGACT TTCCACGGAC AACAGAGCAT CAGGTCCGTC
    ATAGACACAA TGCAACATGC TGTCTACGTC TATGACGTCT GTCATGTAGT CATTGACAAC
    TTGCAGTTCA TGATGGGTCA CGAGCAGCTC TCCTCTGACA GGATTGCAGC TCAAGACTAC
    ATTGTTGGAG CCTTTCGGAA GTTTGCTACA GACAACAGTT GCCACGTGAC TCTGGTCATT
    CACCCTCGGA AAGAAGACGA CGACAAGGAA CTGCAGACAG CATCCATTTT CGGCTCAGCC
    AAAGCAAGCC AAGAAGCAGA CAATGTTCTG ATCTTACAGG ATAGGAAACT GGTGACTGGG
    CCTGGGAAAC GGTATCTGCA GGTGTCCAAG AATCGCTTTG ATGGAGACGT AGGTGTCTTC
    CCACTGGAGT TCAACAAGAA TTCCCTTACC TTCTCCATCC CACCCAAGAG CAAAGCCCGA
    CTCAAGAAGA TCAAGGACGA CAATGGACTA GTGGCCAAAA AATCCTCTTC TGGCAAAAAA
    GGGGCTGCGC ACCAGAACCC TGAGATTTGC TTAGGCCAGG ACCCCAGCCC CGCCCAGCCA
    GATACCTCCA AGTCCTCAGG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu14986
    Clone ID Related Accession (Same CDS sequence) NM_153796.3 , NM_153796.4
    Accession Version NM_153796.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-08-04
    Organism Mus musculus(House mouse)
    Product twinkle protein, mitochondrial
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGCTTC TTCTCCGACG TGCATATCCC CTCCGGATCT TGCTGCCACT GCGTGGCGAG 
    TGGGTGGGTA GGAGGGGCCT GCCCAGAAGC TTGGCGCCAG GCCCTCCCCG CAGAAGGTAC
    AGAAAAGAAG CGCTCCCAGC CTTAGAGATG CCAGTGTCGC CGGTGACTAC GACTGAAATC
    CGCCAGTATT TACGTGCACA TGGGATCCCC TTCCAGGATG GCCACAGCTG CCTTCGAGCA
    CCAAGTCCCT TTGTGGTGTC CTCAGACATC AAAAATGAGA AAAAGGACGC TCCCACTTCC
    TTCTGCCTCT TCATTGACAA GACCACAGGA CACTTTCTTT GCATGACCAG CCTAGCAGAA
    GGGAGCTGGG AAGACTTGCA GGCCAGTGTG GAGGGACGAG GGGATGGGGC CAAAGAGGGT
    GTCCTACTTA GGGAAGGCCC AGAAGCTGAG GTTAGGGAGG AGGTCTTGAG GATCTGGAAC
    CGAGCCATAC CTCTTTGGGA GCTTCCAGAC CCTGAGGAAG CCCAGCTAGC TCGTGTGATG
    TTTGGCCTTA CCAAGGTGAC AGATGACACA CTCAGGCGAT TCAGTGTCCG CTATCTTCGA
    TCTGCTCGAA GTCTGGTCTT CCCTTGGTTC ACCCCTGGGA GCTCAGGATT ACGTGGCCTG
    AAGCTACTAG GGGCTGAGGG TCAGGAAAAT GGAGTACAGT ATGTAGAGAC CACCATCCCC
    CGGCCTGGTG TCTATCACAA CCTATTTGGA TTACCACTGA TCAGCCGGAG AGATACTGAG
    GTGGTTGTGA CAAGTCGTGA ACTGGACAGC CTGGCCTTGA GCCAGTCGAC GGGGCTGCCA
    ACCCTTTCCC TACCCCGAGG GACAGTCTGC TTACCCCCAG CCTTACTGCC TTACCTTGAA
    CAGTTCCGTC GGATTGTGTT CTGGTTAGGA GATGACCTTC GATCCTGGGA AGCTGCCAAA
    CTGTTTGCTC GAAAACTCAA CCCCAAGCGA TGCTCTCTGG TGAGGCCTGG GAACCAACAG
    CCTCGTCCCC TGGAGGCCTT AAACCAAGGC TTAAGTCTTC CCCGTATTCT TCGTACCGCC
    CTACCTGCCT GGCACAAGTC TATTGTGTCT TTTCGGCAGC TCCGAGAGGA GGTTTTAGGA
    GAACTGTCAA ATGTAGAGCA AGCGGCTGGT GTCCGTTGGA GCCGCTTCCC AGACCTGAAT
    CGTCTGCTGA AGGGACATCG GAAGGGCGAG TTGACGGTCT TTACAGGGCC AACAGGCAGT
    GGCAAGACAA CATTCATCAG TGAGTATGCC CTGGACTTGT GTACCCAGGG AGTGAACACA
    CTGTGGGGTA GCTTTGAGAT CAGCAACGTG AGACTAGCCC GGGTCATGCT GACCCAGTTT
    GCTGTGACAC GGCTGGAAGA GCAACTGGAC AAGTATGAAG AGTGGGCAGA CCGTTTTGAG
    GACCTGCCTC TCTACTTCAT GACTTTCCAC GGACAACAGA GCATCAGGTC CGTCATAGAC
    ACAATGCAAC ATGCTGTCTA CGTCTATGAC GTCTGTCATG TAGTCATTGA CAACTTGCAG
    TTCATGATGG GTCACGAGCA GCTCTCCTCT GACAGGATTG CAGCTCAAGA CTACATTGTT
    GGAGCCTTTC GGAAGTTTGC TACAGACAAC AGTTGCCACG TGACTCTGGT CATTCACCCT
    CGGAAAGAAG ACGACGACAA GGAACTGCAG ACAGCATCCA TTTTCGGCTC AGCCAAAGCA
    AGCCAAGAAG CAGACAATGT TCTGATCTTA CAGGATAGGA AACTGGTGAC TGGGCCTGGG
    AAACGGTATC TGCAGGTGTC CAAGAATCGC TTTGATGGAG ACGTAGGTGT CTTCCCACTG
    GAGTTCAACA AGAATTCCCT TACCTTCTCC ATCCCACCCA AGAGCAAAGC CCGACTCAAG
    AAGATCAAGG ACGACAATGG ACTAGTGGCC AAAAAATCCT CTTCTGGCAA AAAAGGGGCT
    GCGCACCAGA ACCCTGAGAT TTGCTTAGGC CAGGACCCCA GCCCCGCCCA GCCAGATACC
    TCCAAGTCCT CAGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_722491.2
    Translation

    Target ORF information:

    RefSeq Version NM_153796.3
    Organism Mus musculus(House mouse)
    Definition Mus musculus progressive external ophthalmoplegia 1 (human) (Peo1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_153796.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGCTTC TTCTCCGACG TGCATATCCC CTCCGGATCT TGCTGCCACT GCGTGGCGAG 
    TGGGTGGGTA GGAGGGGCCT GCCCAGAAGC TTGGCGCCAG GCCCTCCCCG CAGAAGGTAC
    AGAAAAGAAG CGCTCCCAGC CTTAGAGATG CCAGTGTCGC CGGTGACTAC GACTGAAATC
    CGCCAGTATT TACGTGCACA TGGGATCCCC TTCCAGGATG GCCACAGCTG CCTTCGAGCA
    CCAAGTCCCT TTGTGGTGTC CTCAGACATC AAAAATGAGA AAAAGGACGC TCCCACTTCC
    TTCTGCCTCT TCATTGACAA GACCACAGGA CACTTTCTTT GCATGACCAG CCTAGCAGAA
    GGGAGCTGGG AAGACTTGCA GGCCAGTGTG GAGGGACGAG GGGATGGGGC CAAAGAGGGT
    GTCCTACTTA GGGAAGGCCC AGAAGCTGAG GTTAGGGAGG AGGTCTTGAG GATCTGGAAC
    CGAGCCATAC CTCTTTGGGA GCTTCCAGAC CCTGAGGAAG CCCAGCTAGC TCGTGTGATG
    TTTGGCCTTA CCAAGGTGAC AGATGACACA CTCAGGCGAT TCAGTGTCCG CTATCTTCGA
    TCTGCTCGAA GTCTGGTCTT CCCTTGGTTC ACCCCTGGGA GCTCAGGATT ACGTGGCCTG
    AAGCTACTAG GGGCTGAGGG TCAGGAAAAT GGAGTACAGT ATGTAGAGAC CACCATCCCC
    CGGCCTGGTG TCTATCACAA CCTATTTGGA TTACCACTGA TCAGCCGGAG AGATACTGAG
    GTGGTTGTGA CAAGTCGTGA ACTGGACAGC CTGGCCTTGA GCCAGTCGAC GGGGCTGCCA
    ACCCTTTCCC TACCCCGAGG GACAGTCTGC TTACCCCCAG CCTTACTGCC TTACCTTGAA
    CAGTTCCGTC GGATTGTGTT CTGGTTAGGA GATGACCTTC GATCCTGGGA AGCTGCCAAA
    CTGTTTGCTC GAAAACTCAA CCCCAAGCGA TGCTCTCTGG TGAGGCCTGG GAACCAACAG
    CCTCGTCCCC TGGAGGCCTT AAACCAAGGC TTAAGTCTTC CCCGTATTCT TCGTACCGCC
    CTACCTGCCT GGCACAAGTC TATTGTGTCT TTTCGGCAGC TCCGAGAGGA GGTTTTAGGA
    GAACTGTCAA ATGTAGAGCA AGCGGCTGGT GTCCGTTGGA GCCGCTTCCC AGACCTGAAT
    CGTCTGCTGA AGGGACATCG GAAGGGCGAG TTGACGGTCT TTACAGGGCC AACAGGCAGT
    GGCAAGACAA CATTCATCAG TGAGTATGCC CTGGACTTGT GTACCCAGGG AGTGAACACA
    CTGTGGGGTA GCTTTGAGAT CAGCAACGTG AGACTAGCCC GGGTCATGCT GACCCAGTTT
    GCTGTGACAC GGCTGGAAGA GCAACTGGAC AAGTATGAAG AGTGGGCAGA CCGTTTTGAG
    GACCTGCCTC TCTACTTCAT GACTTTCCAC GGACAACAGA GCATCAGGTC CGTCATAGAC
    ACAATGCAAC ATGCTGTCTA CGTCTATGAC GTCTGTCATG TAGTCATTGA CAACTTGCAG
    TTCATGATGG GTCACGAGCA GCTCTCCTCT GACAGGATTG CAGCTCAAGA CTACATTGTT
    GGAGCCTTTC GGAAGTTTGC TACAGACAAC AGTTGCCACG TGACTCTGGT CATTCACCCT
    CGGAAAGAAG ACGACGACAA GGAACTGCAG ACAGCATCCA TTTTCGGCTC AGCCAAAGCA
    AGCCAAGAAG CAGACAATGT TCTGATCTTA CAGGATAGGA AACTGGTGAC TGGGCCTGGG
    AAACGGTATC TGCAGGTGTC CAAGAATCGC TTTGATGGAG ACGTAGGTGT CTTCCCACTG
    GAGTTCAACA AGAATTCCCT TACCTTCTCC ATCCCACCCA AGAGCAAAGC CCGACTCAAG
    AAGATCAAGG ACGACAATGG ACTAGTGGCC AAAAAATCCT CTTCTGGCAA AAAAGGGGCT
    GCGCACCAGA ACCCTGAGAT TTGCTTAGGC CAGGACCCCA GCCCCGCCCA GCCAGATACC
    TCCAAGTCCT CAGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu14986
    Clone ID Related Accession (Same CDS sequence) NM_153796.3 , NM_153796.4
    Accession Version NM_153796.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-09
    Organism Mus musculus(house mouse)
    Product twinkle protein, mitochondrial isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132957.3, CA534850.1, BF225236.1, CA537258.1, AK054159.1 and AV002195.1. On Feb 8, 2017 this sequence version replaced NM_153796.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGCTTC TTCTCCGACG TGCATATCCC CTCCGGATCT TGCTGCCACT GCGTGGCGAG 
    TGGGTGGGTA GGAGGGGCCT GCCCAGAAGC TTGGCGCCAG GCCCTCCCCG CAGAAGGTAC
    AGAAAAGAAG CGCTCCCAGC CTTAGAGATG CCAGTGTCGC CGGTGACTAC GACTGAAATC
    CGCCAGTATT TACGTGCACA TGGGATCCCC TTCCAGGATG GCCACAGCTG CCTTCGAGCA
    CCAAGTCCCT TTGTGGTGTC CTCAGACATC AAAAATGAGA AAAAGGACGC TCCCACTTCC
    TTCTGCCTCT TCATTGACAA GACCACAGGA CACTTTCTTT GCATGACCAG CCTAGCAGAA
    GGGAGCTGGG AAGACTTGCA GGCCAGTGTG GAGGGACGAG GGGATGGGGC CAAAGAGGGT
    GTCCTACTTA GGGAAGGCCC AGAAGCTGAG GTTAGGGAGG AGGTCTTGAG GATCTGGAAC
    CGAGCCATAC CTCTTTGGGA GCTTCCAGAC CCTGAGGAAG CCCAGCTAGC TCGTGTGATG
    TTTGGCCTTA CCAAGGTGAC AGATGACACA CTCAGGCGAT TCAGTGTCCG CTATCTTCGA
    TCTGCTCGAA GTCTGGTCTT CCCTTGGTTC ACCCCTGGGA GCTCAGGATT ACGTGGCCTG
    AAGCTACTAG GGGCTGAGGG TCAGGAAAAT GGAGTACAGT ATGTAGAGAC CACCATCCCC
    CGGCCTGGTG TCTATCACAA CCTATTTGGA TTACCACTGA TCAGCCGGAG AGATACTGAG
    GTGGTTGTGA CAAGTCGTGA ACTGGACAGC CTGGCCTTGA GCCAGTCGAC GGGGCTGCCA
    ACCCTTTCCC TACCCCGAGG GACAGTCTGC TTACCCCCAG CCTTACTGCC TTACCTTGAA
    CAGTTCCGTC GGATTGTGTT CTGGTTAGGA GATGACCTTC GATCCTGGGA AGCTGCCAAA
    CTGTTTGCTC GAAAACTCAA CCCCAAGCGA TGCTCTCTGG TGAGGCCTGG GAACCAACAG
    CCTCGTCCCC TGGAGGCCTT AAACCAAGGC TTAAGTCTTC CCCGTATTCT TCGTACCGCC
    CTACCTGCCT GGCACAAGTC TATTGTGTCT TTTCGGCAGC TCCGAGAGGA GGTTTTAGGA
    GAACTGTCAA ATGTAGAGCA AGCGGCTGGT GTCCGTTGGA GCCGCTTCCC AGACCTGAAT
    CGTCTGCTGA AGGGACATCG GAAGGGCGAG TTGACGGTCT TTACAGGGCC AACAGGCAGT
    GGCAAGACAA CATTCATCAG TGAGTATGCC CTGGACTTGT GTACCCAGGG AGTGAACACA
    CTGTGGGGTA GCTTTGAGAT CAGCAACGTG AGACTAGCCC GGGTCATGCT GACCCAGTTT
    GCTGTGACAC GGCTGGAAGA GCAACTGGAC AAGTATGAAG AGTGGGCAGA CCGTTTTGAG
    GACCTGCCTC TCTACTTCAT GACTTTCCAC GGACAACAGA GCATCAGGTC CGTCATAGAC
    ACAATGCAAC ATGCTGTCTA CGTCTATGAC GTCTGTCATG TAGTCATTGA CAACTTGCAG
    TTCATGATGG GTCACGAGCA GCTCTCCTCT GACAGGATTG CAGCTCAAGA CTACATTGTT
    GGAGCCTTTC GGAAGTTTGC TACAGACAAC AGTTGCCACG TGACTCTGGT CATTCACCCT
    CGGAAAGAAG ACGACGACAA GGAACTGCAG ACAGCATCCA TTTTCGGCTC AGCCAAAGCA
    AGCCAAGAAG CAGACAATGT TCTGATCTTA CAGGATAGGA AACTGGTGAC TGGGCCTGGG
    AAACGGTATC TGCAGGTGTC CAAGAATCGC TTTGATGGAG ACGTAGGTGT CTTCCCACTG
    GAGTTCAACA AGAATTCCCT TACCTTCTCC ATCCCACCCA AGAGCAAAGC CCGACTCAAG
    AAGATCAAGG ACGACAATGG ACTAGTGGCC AAAAAATCCT CTTCTGGCAA AAAAGGGGCT
    GCGCACCAGA ACCCTGAGAT TTGCTTAGGC CAGGACCCCA GCCCCGCCCA GCCAGATACC
    TCCAAGTCCT CAGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_722491.2
    CDS573..2630
    Translation

    Target ORF information:

    RefSeq Version NM_153796.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus twinkle mtDNA helicase (Twnk), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_153796.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGCTTC TTCTCCGACG TGCATATCCC CTCCGGATCT TGCTGCCACT GCGTGGCGAG 
    TGGGTGGGTA GGAGGGGCCT GCCCAGAAGC TTGGCGCCAG GCCCTCCCCG CAGAAGGTAC
    AGAAAAGAAG CGCTCCCAGC CTTAGAGATG CCAGTGTCGC CGGTGACTAC GACTGAAATC
    CGCCAGTATT TACGTGCACA TGGGATCCCC TTCCAGGATG GCCACAGCTG CCTTCGAGCA
    CCAAGTCCCT TTGTGGTGTC CTCAGACATC AAAAATGAGA AAAAGGACGC TCCCACTTCC
    TTCTGCCTCT TCATTGACAA GACCACAGGA CACTTTCTTT GCATGACCAG CCTAGCAGAA
    GGGAGCTGGG AAGACTTGCA GGCCAGTGTG GAGGGACGAG GGGATGGGGC CAAAGAGGGT
    GTCCTACTTA GGGAAGGCCC AGAAGCTGAG GTTAGGGAGG AGGTCTTGAG GATCTGGAAC
    CGAGCCATAC CTCTTTGGGA GCTTCCAGAC CCTGAGGAAG CCCAGCTAGC TCGTGTGATG
    TTTGGCCTTA CCAAGGTGAC AGATGACACA CTCAGGCGAT TCAGTGTCCG CTATCTTCGA
    TCTGCTCGAA GTCTGGTCTT CCCTTGGTTC ACCCCTGGGA GCTCAGGATT ACGTGGCCTG
    AAGCTACTAG GGGCTGAGGG TCAGGAAAAT GGAGTACAGT ATGTAGAGAC CACCATCCCC
    CGGCCTGGTG TCTATCACAA CCTATTTGGA TTACCACTGA TCAGCCGGAG AGATACTGAG
    GTGGTTGTGA CAAGTCGTGA ACTGGACAGC CTGGCCTTGA GCCAGTCGAC GGGGCTGCCA
    ACCCTTTCCC TACCCCGAGG GACAGTCTGC TTACCCCCAG CCTTACTGCC TTACCTTGAA
    CAGTTCCGTC GGATTGTGTT CTGGTTAGGA GATGACCTTC GATCCTGGGA AGCTGCCAAA
    CTGTTTGCTC GAAAACTCAA CCCCAAGCGA TGCTCTCTGG TGAGGCCTGG GAACCAACAG
    CCTCGTCCCC TGGAGGCCTT AAACCAAGGC TTAAGTCTTC CCCGTATTCT TCGTACCGCC
    CTACCTGCCT GGCACAAGTC TATTGTGTCT TTTCGGCAGC TCCGAGAGGA GGTTTTAGGA
    GAACTGTCAA ATGTAGAGCA AGCGGCTGGT GTCCGTTGGA GCCGCTTCCC AGACCTGAAT
    CGTCTGCTGA AGGGACATCG GAAGGGCGAG TTGACGGTCT TTACAGGGCC AACAGGCAGT
    GGCAAGACAA CATTCATCAG TGAGTATGCC CTGGACTTGT GTACCCAGGG AGTGAACACA
    CTGTGGGGTA GCTTTGAGAT CAGCAACGTG AGACTAGCCC GGGTCATGCT GACCCAGTTT
    GCTGTGACAC GGCTGGAAGA GCAACTGGAC AAGTATGAAG AGTGGGCAGA CCGTTTTGAG
    GACCTGCCTC TCTACTTCAT GACTTTCCAC GGACAACAGA GCATCAGGTC CGTCATAGAC
    ACAATGCAAC ATGCTGTCTA CGTCTATGAC GTCTGTCATG TAGTCATTGA CAACTTGCAG
    TTCATGATGG GTCACGAGCA GCTCTCCTCT GACAGGATTG CAGCTCAAGA CTACATTGTT
    GGAGCCTTTC GGAAGTTTGC TACAGACAAC AGTTGCCACG TGACTCTGGT CATTCACCCT
    CGGAAAGAAG ACGACGACAA GGAACTGCAG ACAGCATCCA TTTTCGGCTC AGCCAAAGCA
    AGCCAAGAAG CAGACAATGT TCTGATCTTA CAGGATAGGA AACTGGTGAC TGGGCCTGGG
    AAACGGTATC TGCAGGTGTC CAAGAATCGC TTTGATGGAG ACGTAGGTGT CTTCCCACTG
    GAGTTCAACA AGAATTCCCT TACCTTCTCC ATCCCACCCA AGAGCAAAGC CCGACTCAAG
    AAGATCAAGG ACGACAATGG ACTAGTGGCC AAAAAATCCT CTTCTGGCAA AAAAGGGGCT
    GCGCACCAGA ACCCTGAGAT TTGCTTAGGC CAGGACCCCA GCCCCGCCCA GCCAGATACC
    TCCAAGTCCT CAGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.