Opalin cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Opalin
Entrez Gene ID	226115
Full Name	oligodendrocytic myelin paranodal and inner loop protein
Synonyms	Tmem10,Tmp10
General protein information	Preferred Names oligodendrocytic myelin paranodal and inner loop protein Names opalin transmembrane protein 10 transmembrane protein TMP10
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_153520.1	NP_705740.1	opalin
NM_153520.2	NP_705740.1	opalin

Pathways from BioSystems

Homologies

Homo sapiens (human)	OPALIN	NP_149984.1
Pan troglodytes (chimpanzee)	OPALIN	NP_001238889.1
Canis lupus familiaris (dog)	OPALIN	XP_850811.1
Mus musculus (house mouse)	Opalin	NP_705740.1
	OPALIN	XP_001093096.2
Bos taurus (cattle)	OPALIN	NP_001069891.1
Rattus norvegicus (Norway rat)	Opalin	NP_001017386.1

Gene Ontology
Bibliography

Related articles in PubMed

Motor phenotypes and molecular networks associated with germline deficiency of Ciz1.
Xiao J, Vemula SR, Xue Y, Khan MM, Kuruvilla KP, Marquez-Lona EM, Cobb MR, LeDoux MS
Experimental neurology283(Pt A)110-20(2016 Sep)

Functional Characterization of DNA Methylation in the Oligodendrocyte Lineage.
Moyon S, Huynh JL, Dutta D, Zhang F, Ma D, Yoo S, Lawrence R, Wegner M, John GR, Emery B, Lubetzki C, Franklin RJ, Fan G, Zhu J, Dupree JL, Casaccia P
Cell reports(2016 Apr)

Mammalian-Specific Central Myelin Protein Opalin Is Redundant for Normal Myelination: Structural and Behavioral Assessments.
Yoshikawa F, Sato Y, Tohyama K, Akagi T, Furuse T, Sadakata T, Tanaka M, Shinoda Y, Hashikawa T, Itohara S, Sano Y, Ghandour MS, Wakana S, Furuichi T
PloS one11(11)e0166732(2016)

Oligodendrocyte precursor cell-intrinsic effect of Rheb1 controls differentiation and mediates mTORC1-dependent myelination in brain.
Zou Y, Jiang W, Wang J, Li Z, Zhang J, Bu J, Zou J, Zhou L, Yu S, Cui Y, Yang W, Luo L, Lu QR, Liu Y, Chen M, Worley PF, Xiao B
The Journal of neuroscience : the official journal of the Society for Neuroscience34(47)15764-78(2014 Nov)

Gene and MicroRNA transcriptome analysis of Parkinson's related LRRK2 mouse models.
Dorval V, Mandemakers W, Jolivette F, Coudert L, Mazroui R, De Strooper B, H?bert SS
PloS one9(1)e85510(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study demonstrates that Tmem10 can be used as a specific marker for myelinating oligodendrocytes and perhaps for the evaluation of myelination diseases, such as multiple sclerosis
Title: Identification of Tmem10 as a novel late-stage oligodendrocytes marker for detecting hypomyelination.

Tmem10 plays a possible role in oligodendrocyte differentiation and myelination of the central nervous system.
Title: Identification of Tmem10/Opalin as an oligodendrocyte enriched gene using expression profiling combined with genetic cell ablation.

highly sialylglycosylated Opalin has a role in an intermembranous function of the myelin paranodal loops in the central nervous system
Title: Opalin, a transmembrane sialylglycoprotein located in the central nervous system myelin paranodal loop membrane.

Together, our results demonstrate that Tmem10 is a novel marker for in vitro generated oligodendrocytes.
Title: Identification of Tmem10/Opalin as a novel marker for oligodendrocytes using gene expression profiling.

The following Opalin gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Opalin cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02379	NM_153520.2 Latest version!	Mus musculus oligodendrocytic myelin paranodal and inner loop protein (Opalin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OMu02379	NM_153520.1	Mus musculus oligodendrocytic myelin paranodal and inner loop protein (Opalin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Opalin ( NM_153520.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_705740.1 Mus musculus oligodendrocytic myelin paranodal and inner loop protein (Opalin), mRNA.

Price & Availability↑

CloneID	OMu02379
Clone ID Related Accession (Same CDS sequence)	NM_153520.1 , NM_153520.2
Accession Version	NM_153520.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-14
Organism	Mus musculus(house mouse)
Product	opalin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BK000002.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BK000002.1, AK140031.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849383 [ECO:0000350] ##Evidence-Data-END##

ATGAGTTTTT CACTGAACTT TACACTGCCA TCGAATACGA CATCCTCTCC AGTTGTCACG 
AGTGCCAAAG CCACAGACTG TGGTCCCTCT ATTGGACTGG CAGCCGGAAT CCCATCCCTG 
CTGGCCACAG CCCTGCTGGT GGCTTTGCTG TTTACCTTGA TCCAGCGAAG AAGAACCATC 
GATGATGAGC CCGTAGAGGA GACTGAGATA CCATGTGAAA TTTCAGAACT CTATGACAAT 
CCCAAAATAT CTGAGAATCC TAGGCGGTCA CCCACACATG AAATGAACCC ACGGGGCAGC 
CAAGAAGGGC ACATATATGT GAAGACTGTC TCGGGAAGCG AGGAGCCCCT GCCGGACACC 
TACCGTCCTC CTGAAGAACT AGAAAGGAGA AGAGGACTTT GGTGGCTTGT ACCCAGCCTG 
AGCCTAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705740.1
CDS	78..509
Translation	MSFSLNFTLPSNTTSSPVVTSAKATDCGPSIGLAAGIPSLLATALLVALLFTLIQRRRTIDDEPVEETEIPCEISELYDNPKISENPRRSPTHEMNPRGSQEGHIYVKTVSGSEEPLPDTYRPPEELERRRGLWWLVPSLSLE

Target ORF information:

RefSeq Version	NM_153520.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus oligodendrocytic myelin paranodal and inner loop protein (Opalin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153520.1

ORF Insert Sequence:

ATGAGTTTTT CACTGAACTT TACACTGCCA TCGAATACGA CATCCTCTCC AGTTGTCACG 
AGTGCCAAAG CCACAGACTG TGGTCCCTCT ATTGGACTGG CAGCCGGAAT CCCATCCCTG 
CTGGCCACAG CCCTGCTGGT GGCTTTGCTG TTTACCTTGA TCCAGCGAAG AAGAACCATC 
GATGATGAGC CCGTAGAGGA GACTGAGATA CCATGTGAAA TTTCAGAACT CTATGACAAT 
CCCAAAATAT CTGAGAATCC TAGGCGGTCA CCCACACATG AAATGAACCC ACGGGGCAGC 
CAAGAAGGGC ACATATATGT GAAGACTGTC TCGGGAAGCG AGGAGCCCCT GCCGGACACC 
TACCGTCCTC CTGAAGAACT AGAAAGGAGA AGAGGACTTT GGTGGCTTGT ACCCAGCCTG 
AGCCTAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Opalin ( NM_153520.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_705740.1 Mus musculus oligodendrocytic myelin paranodal and inner loop protein (Opalin), mRNA.

Price & Availability↑

CloneID	OMu02379
Clone ID Related Accession (Same CDS sequence)	NM_153520.1 , NM_153520.2
Accession Version	NM_153520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Mus musculus(house mouse)
Product	opalin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121873.3. On Feb 7, 2019 this sequence version replaced NM_153520.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK140031.1, BK000002.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849383 [ECO:0000350] ##Evidence-Data-END##

ATGAGTTTTT CACTGAACTT TACACTGCCA TCGAATACGA CATCCTCTCC AGTTGTCACG 
AGTGCCAAAG CCACAGACTG TGGTCCCTCT ATTGGACTGG CAGCCGGAAT CCCATCCCTG 
CTGGCCACAG CCCTGCTGGT GGCTTTGCTG TTTACCTTGA TCCAGCGAAG AAGAACCATC 
GATGATGAGC CCGTAGAGGA GACTGAGATA CCATGTGAAA TTTCAGAACT CTATGACAAT 
CCCAAAATAT CTGAGAATCC TAGGCGGTCA CCCACACATG AAATGAACCC ACGGGGCAGC 
CAAGAAGGGC ACATATATGT GAAGACTGTC TCGGGAAGCG AGGAGCCCCT GCCGGACACC 
TACCGTCCTC CTGAAGAACT AGAAAGGAGA AGAGGACTTT GGTGGCTTGT ACCCAGCCTG 
AGCCTAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705740.1
CDS	78..509
Translation	MSFSLNFTLPSNTTSSPVVTSAKATDCGPSIGLAAGIPSLLATALLVALLFTLIQRRRTIDDEPVEETEIPCEISELYDNPKISENPRRSPTHEMNPRGSQEGHIYVKTVSGSEEPLPDTYRPPEELERRRGLWWLVPSLSLE

Target ORF information:

RefSeq Version	NM_153520.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus oligodendrocytic myelin paranodal and inner loop protein (Opalin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153520.2

ORF Insert Sequence:

ATGAGTTTTT CACTGAACTT TACACTGCCA TCGAATACGA CATCCTCTCC AGTTGTCACG 
AGTGCCAAAG CCACAGACTG TGGTCCCTCT ATTGGACTGG CAGCCGGAAT CCCATCCCTG 
CTGGCCACAG CCCTGCTGGT GGCTTTGCTG TTTACCTTGA TCCAGCGAAG AAGAACCATC 
GATGATGAGC CCGTAGAGGA GACTGAGATA CCATGTGAAA TTTCAGAACT CTATGACAAT 
CCCAAAATAT CTGAGAATCC TAGGCGGTCA CCCACACATG AAATGAACCC ACGGGGCAGC 
CAAGAAGGGC ACATATATGT GAAGACTGTC TCGGGAAGCG AGGAGCCCCT GCCGGACACC 
TACCGTCCTC CTGAAGAACT AGAAAGGAGA AGAGGACTTT GGTGGCTTGT ACCCAGCCTG 
AGCCTAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Motor phenotypes and molecular networks associated with germline deficiency of Ciz1.
Experimental neurology283(Pt A)110-20(2016 Sep)
Xiao J,Vemula SR,Xue Y,Khan MM,Kuruvilla KP,Marquez-Lona EM,Cobb MR,LeDoux MS

Functional Characterization of DNA Methylation in the Oligodendrocyte Lineage.
Cell reports(2016 Apr)
Moyon S,Huynh JL,Dutta D,Zhang F,Ma D,Yoo S,Lawrence R,Wegner M,John GR,Emery B,Lubetzki C,Franklin RJ,Fan G,Zhu J,Dupree JL,Casaccia P

Mammalian-Specific Central Myelin Protein Opalin Is Redundant for Normal Myelination: Structural and Behavioral Assessments.
PloS one11(11)e0166732(2016)
Yoshikawa F,Sato Y,Tohyama K,Akagi T,Furuse T,Sadakata T,Tanaka M,Shinoda Y,Hashikawa T,Itohara S,Sano Y,Ghandour MS,Wakana S,Furuichi T

Oligodendrocyte precursor cell-intrinsic effect of Rheb1 controls differentiation and mediates mTORC1-dependent myelination in brain.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(47)15764-78(2014 Nov)
Zou Y,Jiang W,Wang J,Li Z,Zhang J,Bu J,Zou J,Zhou L,Yu S,Cui Y,Yang W,Luo L,Lu QR,Liu Y,Chen M,Worley PF,Xiao B

Gene and MicroRNA transcriptome analysis of Parkinson's related LRRK2 mouse models.
PloS one9(1)e85510(2014)
Dorval V,Mandemakers W,Jolivette F,Coudert L,Mazroui R,De Strooper B,H?bert SS

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Opalin cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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