Mib1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mib1
Entrez Gene ID	225164
Full Name	mindbomb E3 ubiquitin protein ligase 1
Synonyms	DIP-1,E430019M12Rik,Mib,mKIAA1323
General protein information	Preferred Names mindbomb E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase MIB1 DAPK-interacting protein-1 RING-type E3 ubiquitin transferase MIB1 mind bomb homolog 1 mind bomb-1 mindbomb homolog 1 skeletrophin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006525811.4	XP_006525874.1	E3 ubiquitin-protein ligase MIB1 isoform X1
NM_144860.3	NP_659109.2	E3 ubiquitin-protein ligase MIB1
NM_001364997.1	NP_001351926.1	E3 ubiquitin-protein ligase MIB1
NM_144860.2	NP_659109.2	E3 ubiquitin-protein ligase MIB1
XM_006525811.3	XP_006525874.1	E3 ubiquitin-protein ligase MIB1 isoform X1
XM_006525810.2	XP_006525873.1	E3 ubiquitin-protein ligase MIB1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MIB1	NP_065825.1
Mus musculus (house mouse)	Mib1	NP_659109.2
Gallus gallus (chicken)	MIB1	XP_419157.3
Danio rerio (zebrafish)	mib	NP_775393.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011932	XP_320601.4
Canis lupus familiaris (dog)	MIB1	XP_547643.4
Drosophila melanogaster (fruit fly)	mib1	NP_648826.2
Xenopus tropicalis (tropical clawed frog)	mib1	NP_001123407.1
Pan troglodytes (chimpanzee)	MIB1	XP_003315915.1
	MIB1	XP_001092086.1
Bos taurus (cattle)	MIB1	NP_001192959.1
Rattus norvegicus (Norway rat)	Mib1	NP_001100875.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of Usp9x disrupts cell adhesion, and components of the Wnt and Notch signaling pathways in neural progenitors.
Premarathne S, Murtaza M, Matigian N, Jolly LA, Wood SA
Scientific reports7(1)8109(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mib1 is necessary for the Notch activation in adjacent retinal progenitor cell (RPC)The retinal pigment epithelium (RPE) expresses active Notch ligands for adjacent RPC
Title: The Retinal Pigment Epithelium Is a Notch Signaling Niche in the Mouse Retina.

Deletion of Mib1 resulted in an increase in the fraction of pendrin positive intercalated cells in the kidney.
Title: Altered response of pendrin-positive intercalated cells in the kidney of Hoxb7-Cre;Mib1f/f mice.

Loss of MIB1 and dismantling of the apical secretory apparatus was the earliest quantifiable aberration in zymogenic cells undergoing transition to a precancerous metaplastic state in mouse.
Title: The ubiquitin ligase Mindbomb 1 coordinates gastrointestinal secretory cell maturation.

Mib1 is a major component of the signal-sending cells required to provide Notch ligand activity for specifying neurons and glia in the spinal cord
Title: The E3 ligase Mind bomb-1 (Mib1) modulates Delta-Notch signaling to control neurogenesis and gliogenesis in the developing spinal cord.

In contrast to delta-like 1 (Dll1) protein, delta-like 4 (deltall4) function relies on a combination of factors, including strong Mindbomb1 (Mib1) association, ubiquitination, and internalization and recycling back to the cell surface, to engage Notch1 effectively.
Title: Role of recycling, Mindbomb1 association, and exclusion from lipid rafts of ?-like 4 for effective Notch signaling to drive T cell development.

The following Mib1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mib1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18519	NM_001364997.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu18519	NM_144860.3 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu18519	XM_006525811.4 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu18519	XM_006525810.2 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu18519	NM_144860.2	Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu18519	XM_006525811.3	Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mib1 ( NM_144860.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_659109.2 Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18519
Clone ID Related Accession (Same CDS sequence)	XM_006525811.4 , NM_144860.3 , NM_001364997.1 , NM_144860.2 , XM_006525811.3 , XM_006525810.2
Accession Version	NM_144860.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY245539.1 and AC140316.3. On Jul 20, 2018 this sequence version replaced NM_144860.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY245539.1, AY147848.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTAACT CCCGCAACAA CCGAGTGATG GTGGAGGGGG TCGGCGCCCG CGTAGTGCGC 
GGCCCGGACT GGAAGTGGGG CAAGCAGGAC GGCGGCGAGG GCCATGTAGG CACTGTACGG 
AGCTTCGAGA GTCCGGAGGA GGTCGTGGTG GTGTGGGACA ACGGCACGGC CGCCAACTAC 
CGCTGCTCAG GGGCCTACGA CCTGCGTATC CTGGACAGCG CGCCCACCGG CATCAAGCAT 
GATGGAACTA TGTGTGATAC CTGCCGCCAG CAACCAATCA TTGGCATTCG ATGGAAATGT 
GCAGAGTGCA CCAATTATGA TTTGTGCACT GTTTGTTATC ATGGAGATAA ACATCACTTA 
AGGCATCGCT TTTATAGGAT TACTACACCA GGAAGTGAAC GGGTTCTGTT AGAGTCTCGT 
AGAAAATCTA AGAAGATTAC AGCCAGAGGA ATCTTTGCTG GTGCCAGAGT GGTGCGAGGA 
GTGGACTGGC AGTGGGAAGA TCAAGATGGA GGCAATGGGC GTAGAGGAAA GGTCACGGAG 
ATCCAGGACT GGAGTGCATC CAGCCCACAC AGTGCAGCCT ATGTTCTCTG GGATAACGGT 
GCTAAGAACC TTTACAGGGT TGGCTTTGAG GGCATGTCTG ATCTCAAGTG TGTCCAGGAT 
GCCAAAGGAG GTTCTTTCTA CAGAGATCAT TGCCCTGTGC TAGGTGAGCA GAATGGCAAC 
AGGAATCCTG GTGGATTGCA GATTGGTGAC CTGGTAAATA TAGACCTTGA TCTAGAAATT 
GTCCAGTCTT TACAGCACGG TCATGGAGGA TGGACCGATG GCATGTTTGA GACTTTAACT 
ACAACTGGAA CCGTCTGTGG CATTGATGAA GATCATGACA TTGTAGTACA GTATCCAAGT 
GGCAATAGAT GGACTTTCAA TCCTGCTGTT CTCACTAAGG CAAACATTGT CCGAAGTGGG 
GATGCTGCAC AGGGTGCAGA AGGAGGCACC TCACAGTTTC AAGTGGGTGA TCTTGTGCAA 
GTTTGTTATG ATCTGGAAAG AATTAAACTT CTCCAAAGAG GACATGGTGA ATGGGCTGAA 
GCAATGCTTC CAACTTTAGG TAAAGTTGGC CGAGTACAAC AGATTTATTC GGACAGTGAC 
TTAAAGGTGG AAGTCTGTGG AACATCTTGG ACGTATAACC CTGCAGCAGT TTCCAAGGTG 
GCCCCTGCAG GATCAGCCAT TAGCAATGCA TCTGGTGAAA GACTCTCCCA GCTTCTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGTGAC CTCAATGAAG AGCTGGTTAA GGCTGCTGCC 
AATGGAGACG TGGCTAAAGT GGAAGATTTG CTGAAAAGAC CAGATGTGGA TGTAAACGGA 
CAGTGTGCTG GCCACACAGC AATGCAAGCT GCTAGTCAGA ACGGACATGT TGATATTTTG 
AAATTACTTT TGAAGCAAAA TGTTGATGTT GAAGCAGAGG ATAAAGATGG AGATCGAGCC 
GTTCACCATG CAGCCTTTGG AGATGAGGGT GCTGTCATAG AAGTCCTACA CCGAGGCAGT 
GCTGACCTGA ATGCTCGTAA TAAGCGTAGA CAGACGCCTC TTCATATTGC TGTCAATAAA 
GGTCATCTTC AAGTTGTGAA GACTTTGTTG GATTTTGGTT GTCATCCCAG TCTCCAGGAT 
TCTGAAGGTG ACACCCCTCT CCATGATGCC ATAAGTAAGA AACGGGATGA CATTCTGGCA 
GTTCTTCTGG AAGCTGGAGC AGATGTGACC ATCACGAACA ATAACGGGTT CAATGCCCTG 
CACCATGCTG CACTGAGAGG GAACCCCAGT GCAATGCGTG TTTTACTCTC AAAATTACCA 
AGACCATGGA TTGTGGATGA GAAGAAAGAT GATGGTTATA CTGCCTTGCA CCTGGCTGCC 
CTGAATAATC ATGTGGAAGT GGCTGAGCTG TTGGTGCATC AGGGAAATGC AAACCTGGAT 
ATCCAGAATG TGAACCAGCA AACAGCCCTA CACCTTGCTG TTGAACGGCA ACACACCCAG 
ATTGTTAGGC TTTTGGTCCG TGCAGGTGCT AAGCTAGACA TTCAGGATAA GGATGGAGAT 
ACTCCTCTGC ATGAAGCTTT GAGACATCAT ACTTTGTCTC AACTACGTCA GCTACAAGAC 
ATGCAAGATG TGGGCAAGGT AGACGCTGCC TGGGAACCTT CAAAAAACAC ATTAATAATG 
GGACTTGGTA CCCAGGGGGC TGAGAAGAAG AGTGCAGCAT CCATTGCGTG TTTCTTGGCA 
GCCAATGGTG CTGATCTCAG CATTCGAAAT AAGAAGGGTC AGTCACCACT GGATCTCTGT 
CCTGACCCAA GTCTCTGCAA AGCTTTGGCA AAATGTCATA AGGAAAAAGT CAGTGGCCAA 
GTGGGTTCTC GGAGTCCTTC TATGATTAGC AACGATTCTG AAACTTTAGA GGAATGTATG 
GTGTGCTCAG ACATGAAGAG AGATACTCTT TTTGGTCCGT GTGGACACAT TGCAACCTGT 
TCTTTGTGTT CCCCTCGTGT CAAGAAATGC CTCATTTGTA AAGAGCAGGT TCAGTCCAGG 
ACAAAGATTG AAGAATGTGT GGTGTGCTCC GACAAGAAAG CAGCTGTTCT CTTTCAACCT 
TGTGGGCACA TGTGTGCTTG TGAGAACTGT GCCAGCCTGA TGAAAAAGTG TGTGCAGTGT 
CGGGCAGTGG TTGAGCGGAG GGTGCCTTTC ATCACGTGCT GTGGTGGCAA GAGCTCAGAA 
GACCCCTCTG ATGAAATCTC AAGTGGGAAT ATCCCAGTGC TGCAGAAGGA CAAAGACAAC 
ACCAATGTTA ACGCAGATGT GCAGAAACTG CAGCAGCAGC TTCAGGACAT TAAGGAGCAG 
ACCATGTGCC CTGTGTGCTT GGATCGCCTG AAGAACATGA TTTTCCTCTG TGGCCATGGA 
ACATGCCAGC TCTGTGGAGA CCGGATGAGT GAATGCCCAA TTTGTCGCAA GGCAATTGAA 
CGAAGGATTC TTTTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659109.2
CDS	681..3701
Translation	MSNSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFITCCGGKSSEDPSDEISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	NM_144860.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144860.3

ORF Insert Sequence:

ATGAGTAACT CCCGCAACAA CCGAGTGATG GTGGAGGGGG TCGGCGCCCG CGTAGTGCGC 
GGCCCGGACT GGAAGTGGGG CAAGCAGGAC GGCGGCGAGG GCCATGTAGG CACTGTACGG 
AGCTTCGAGA GTCCGGAGGA GGTCGTGGTG GTGTGGGACA ACGGCACGGC CGCCAACTAC 
CGCTGCTCAG GGGCCTACGA CCTGCGTATC CTGGACAGCG CGCCCACCGG CATCAAGCAT 
GATGGAACTA TGTGTGATAC CTGCCGCCAG CAACCAATCA TTGGCATTCG ATGGAAATGT 
GCAGAGTGCA CCAATTATGA TTTGTGCACT GTTTGTTATC ATGGAGATAA ACATCACTTA 
AGGCATCGCT TTTATAGGAT TACTACACCA GGAAGTGAAC GGGTTCTGTT AGAGTCTCGT 
AGAAAATCTA AGAAGATTAC AGCCAGAGGA ATCTTTGCTG GTGCCAGAGT GGTGCGAGGA 
GTGGACTGGC AGTGGGAAGA TCAAGATGGA GGCAATGGGC GTAGAGGAAA GGTCACGGAG 
ATCCAGGACT GGAGTGCATC CAGCCCACAC AGTGCAGCCT ATGTTCTCTG GGATAACGGT 
GCTAAGAACC TTTACAGGGT TGGCTTTGAG GGCATGTCTG ATCTCAAGTG TGTCCAGGAT 
GCCAAAGGAG GTTCTTTCTA CAGAGATCAT TGCCCTGTGC TAGGTGAGCA GAATGGCAAC 
AGGAATCCTG GTGGATTGCA GATTGGTGAC CTGGTAAATA TAGACCTTGA TCTAGAAATT 
GTCCAGTCTT TACAGCACGG TCATGGAGGA TGGACCGATG GCATGTTTGA GACTTTAACT 
ACAACTGGAA CCGTCTGTGG CATTGATGAA GATCATGACA TTGTAGTACA GTATCCAAGT 
GGCAATAGAT GGACTTTCAA TCCTGCTGTT CTCACTAAGG CAAACATTGT CCGAAGTGGG 
GATGCTGCAC AGGGTGCAGA AGGAGGCACC TCACAGTTTC AAGTGGGTGA TCTTGTGCAA 
GTTTGTTATG ATCTGGAAAG AATTAAACTT CTCCAAAGAG GACATGGTGA ATGGGCTGAA 
GCAATGCTTC CAACTTTAGG TAAAGTTGGC CGAGTACAAC AGATTTATTC GGACAGTGAC 
TTAAAGGTGG AAGTCTGTGG AACATCTTGG ACGTATAACC CTGCAGCAGT TTCCAAGGTG 
GCCCCTGCAG GATCAGCCAT TAGCAATGCA TCTGGTGAAA GACTCTCCCA GCTTCTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGTGAC CTCAATGAAG AGCTGGTTAA GGCTGCTGCC 
AATGGAGACG TGGCTAAAGT GGAAGATTTG CTGAAAAGAC CAGATGTGGA TGTAAACGGA 
CAGTGTGCTG GCCACACAGC AATGCAAGCT GCTAGTCAGA ACGGACATGT TGATATTTTG 
AAATTACTTT TGAAGCAAAA TGTTGATGTT GAAGCAGAGG ATAAAGATGG AGATCGAGCC 
GTTCACCATG CAGCCTTTGG AGATGAGGGT GCTGTCATAG AAGTCCTACA CCGAGGCAGT 
GCTGACCTGA ATGCTCGTAA TAAGCGTAGA CAGACGCCTC TTCATATTGC TGTCAATAAA 
GGTCATCTTC AAGTTGTGAA GACTTTGTTG GATTTTGGTT GTCATCCCAG TCTCCAGGAT 
TCTGAAGGTG ACACCCCTCT CCATGATGCC ATAAGTAAGA AACGGGATGA CATTCTGGCA 
GTTCTTCTGG AAGCTGGAGC AGATGTGACC ATCACGAACA ATAACGGGTT CAATGCCCTG 
CACCATGCTG CACTGAGAGG GAACCCCAGT GCAATGCGTG TTTTACTCTC AAAATTACCA 
AGACCATGGA TTGTGGATGA GAAGAAAGAT GATGGTTATA CTGCCTTGCA CCTGGCTGCC 
CTGAATAATC ATGTGGAAGT GGCTGAGCTG TTGGTGCATC AGGGAAATGC AAACCTGGAT 
ATCCAGAATG TGAACCAGCA AACAGCCCTA CACCTTGCTG TTGAACGGCA ACACACCCAG 
ATTGTTAGGC TTTTGGTCCG TGCAGGTGCT AAGCTAGACA TTCAGGATAA GGATGGAGAT 
ACTCCTCTGC ATGAAGCTTT GAGACATCAT ACTTTGTCTC AACTACGTCA GCTACAAGAC 
ATGCAAGATG TGGGCAAGGT AGACGCTGCC TGGGAACCTT CAAAAAACAC ATTAATAATG 
GGACTTGGTA CCCAGGGGGC TGAGAAGAAG AGTGCAGCAT CCATTGCGTG TTTCTTGGCA 
GCCAATGGTG CTGATCTCAG CATTCGAAAT AAGAAGGGTC AGTCACCACT GGATCTCTGT 
CCTGACCCAA GTCTCTGCAA AGCTTTGGCA AAATGTCATA AGGAAAAAGT CAGTGGCCAA 
GTGGGTTCTC GGAGTCCTTC TATGATTAGC AACGATTCTG AAACTTTAGA GGAATGTATG 
GTGTGCTCAG ACATGAAGAG AGATACTCTT TTTGGTCCGT GTGGACACAT TGCAACCTGT 
TCTTTGTGTT CCCCTCGTGT CAAGAAATGC CTCATTTGTA AAGAGCAGGT TCAGTCCAGG 
ACAAAGATTG AAGAATGTGT GGTGTGCTCC GACAAGAAAG CAGCTGTTCT CTTTCAACCT 
TGTGGGCACA TGTGTGCTTG TGAGAACTGT GCCAGCCTGA TGAAAAAGTG TGTGCAGTGT 
CGGGCAGTGG TTGAGCGGAG GGTGCCTTTC ATCACGTGCT GTGGTGGCAA GAGCTCAGAA 
GACCCCTCTG ATGAAATCTC AAGTGGGAAT ATCCCAGTGC TGCAGAAGGA CAAAGACAAC 
ACCAATGTTA ACGCAGATGT GCAGAAACTG CAGCAGCAGC TTCAGGACAT TAAGGAGCAG 
ACCATGTGCC CTGTGTGCTT GGATCGCCTG AAGAACATGA TTTTCCTCTG TGGCCATGGA 
ACATGCCAGC TCTGTGGAGA CCGGATGAGT GAATGCCCAA TTTGTCGCAA GGCAATTGAA 
CGAAGGATTC TTTTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib1 ( NM_001364997.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001351926.1 Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18519
Clone ID Related Accession (Same CDS sequence)	XM_006525811.4 , NM_144860.3 , NM_001364997.1 , NM_144860.2 , XM_006525811.3 , XM_006525810.2
Accession Version	NM_001364997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105947.11 and AC140316.3. On Jul 20, 2018 this sequence version replaced XM_006525810.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY149907.1, AY974091.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGTAACT CCCGCAACAA CCGAGTGATG GTGGAGGGGG TCGGCGCCCG CGTAGTGCGC 
GGCCCGGACT GGAAGTGGGG CAAGCAGGAC GGCGGCGAGG GCCATGTAGG CACTGTACGG 
AGCTTCGAGA GTCCGGAGGA GGTCGTGGTG GTGTGGGACA ACGGCACGGC CGCCAACTAC 
CGCTGCTCAG GGGCCTACGA CCTGCGTATC CTGGACAGCG CGCCCACCGG CATCAAGCAT 
GATGGAACTA TGTGTGATAC CTGCCGCCAG CAACCAATCA TTGGCATTCG ATGGAAATGT 
GCAGAGTGCA CCAATTATGA TTTGTGCACT GTTTGTTATC ATGGAGATAA ACATCACTTA 
AGGCATCGCT TTTATAGGAT TACTACACCA GGAAGTGAAC GGGTTCTGTT AGAGTCTCGT 
AGAAAATCTA AGAAGATTAC AGCCAGAGGA ATCTTTGCTG GTGCCAGAGT GGTGCGAGGA 
GTGGACTGGC AGTGGGAAGA TCAAGATGGA GGCAATGGGC GTAGAGGAAA GGTCACGGAG 
ATCCAGGACT GGAGTGCATC CAGCCCACAC AGTGCAGCCT ATGTTCTCTG GGATAACGGT 
GCTAAGAACC TTTACAGGGT TGGCTTTGAG GGCATGTCTG ATCTCAAGTG TGTCCAGGAT 
GCCAAAGGAG GTTCTTTCTA CAGAGATCAT TGCCCTGTGC TAGGTGAGCA GAATGGCAAC 
AGGAATCCTG GTGGATTGCA GATTGGTGAC CTGGTAAATA TAGACCTTGA TCTAGAAATT 
GTCCAGTCTT TACAGCACGG TCATGGAGGA TGGACCGATG GCATGTTTGA GACTTTAACT 
ACAACTGGAA CCGTCTGTGG CATTGATGAA GATCATGACA TTGTAGTACA GTATCCAAGT 
GGCAATAGAT GGACTTTCAA TCCTGCTGTT CTCACTAAGG CAAACATTGT CCGAAGTGGG 
GATGCTGCAC AGGGTGCAGA AGGAGGCACC TCACAGTTTC AAGTGGGTGA TCTTGTGCAA 
GTTTGTTATG ATCTGGAAAG AATTAAACTT CTCCAAAGAG GACATGGTGA ATGGGCTGAA 
GCAATGCTTC CAACTTTAGG TAAAGTTGGC CGAGTACAAC AGATTTATTC GGACAGTGAC 
TTAAAGGTGG AAGTCTGTGG AACATCTTGG ACGTATAACC CTGCAGCAGT TTCCAAGGTG 
GCCCCTGCAG GATCAGCCAT TAGCAATGCA TCTGGTGAAA GACTCTCCCA GCTTCTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGTGAC CTCAATGAAG AGCTGGTTAA GGCTGCTGCC 
AATGGAGACG TGGCTAAAGT GGAAGATTTG CTGAAAAGAC CAGATGTGGA TGTAAACGGA 
CAGTGTGCTG GCCACACAGC AATGCAAGCT GCTAGTCAGA ACGGACATGT TGATATTTTG 
AAATTACTTT TGAAGCAAAA TGTTGATGTT GAAGCAGAGG ATAAAGATGG AGATCGAGCC 
GTTCACCATG CAGCCTTTGG AGATGAGGGT GCTGTCATAG AAGTCCTACA CCGAGGCAGT 
GCTGACCTGA ATGCTCGTAA TAAGCGTAGA CAGACGCCTC TTCATATTGC TGTCAATAAA 
GGTCATCTTC AAGTTGTGAA GACTTTGTTG GATTTTGGTT GTCATCCCAG TCTCCAGGAT 
TCTGAAGGTG ACACCCCTCT CCATGATGCC ATAAGTAAGA AACGGGATGA CATTCTGGCA 
GTTCTTCTGG AAGCTGGAGC AGATGTGACC ATCACGAACA ATAACGGGTT CAATGCCCTG 
CACCATGCTG CACTGAGAGG GAACCCCAGT GCAATGCGTG TTTTACTCTC AAAATTACCA 
AGACCATGGA TTGTGGATGA GAAGAAAGAT GATGGTTATA CTGCCTTGCA CCTGGCTGCC 
CTGAATAATC ATGTGGAAGT GGCTGAGCTG TTGGTGCATC AGGGAAATGC AAACCTGGAT 
ATCCAGAATG TGAACCAGCA AACAGCCCTA CACCTTGCTG TTGAACGGCA ACACACCCAG 
ATTGTTAGGC TTTTGGTCCG TGCAGGTGCT AAGCTAGACA TTCAGGATAA GGATGGAGAT 
ACTCCTCTGC ATGAAGCTTT GAGACATCAT ACTTTGTCTC AACTACGTCA GCTACAAGAC 
ATGCAAGATG TGGGCAAGGT AGACGCTGCC TGGGAACCTT CAAAAAACAC ATTAATAATG 
GGACTTGGTA CCCAGGGGGC TGAGAAGAAG AGTGCAGCAT CCATTGCGTG TTTCTTGGCA 
GCCAATGGTG CTGATCTCAG CATTCGAAAT AAGAAGGGTC AGTCACCACT GGATCTCTGT 
CCTGACCCAA GTCTCTGCAA AGCTTTGGCA AAATGTCATA AGGAAAAAGT CAGTGGCCAA 
GTGGGTTCTC GGAGTCCTTC TATGATTAGC AACGATTCTG AAACTTTAGA GGAATGTATG 
GTGTGCTCAG ACATGAAGAG AGATACTCTT TTTGGTCCGT GTGGACACAT TGCAACCTGT 
TCTTTGTGTT CCCCTCGTGT CAAGAAATGC CTCATTTGTA AAGAGCAGGT TCAGTCCAGG 
ACAAAGATTG AAGAATGTGT GGTGTGCTCC GACAAGAAAG CAGCTGTTCT CTTTCAACCT 
TGTGGGCACA TGTGTGCTTG TGAGAACTGT GCCAGCCTGA TGAAAAAGTG TGTGCAGTGT 
CGGGCAGTGG TTGAGCGGAG GGTGCCTTTC ATCACGTGCT GTGGTGGCAA GAGCTCAGAA 
GACCCCTCTG ATGAAATCTC AAGTGGGAAT ATCCCAGTGC TGCAGAAGGA CAAAGACAAC 
ACCAATGTTA ACGCAGATGT GCAGAAACTG CAGCAGCAGC TTCAGGACAT TAAGGAGCAG 
ACCATGTGCC CTGTGTGCTT GGATCGCCTG AAGAACATGA TTTTCCTCTG TGGCCATGGA 
ACATGCCAGC TCTGTGGAGA CCGGATGAGT GAATGCCCAA TTTGTCGCAA GGCAATTGAA 
CGAAGGATTC TTTTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351926.1
CDS	681..3701
Translation	MSNSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFITCCGGKSSEDPSDEISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	NM_001364997.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364997.1

ORF Insert Sequence:

ATGAGTAACT CCCGCAACAA CCGAGTGATG GTGGAGGGGG TCGGCGCCCG CGTAGTGCGC 
GGCCCGGACT GGAAGTGGGG CAAGCAGGAC GGCGGCGAGG GCCATGTAGG CACTGTACGG 
AGCTTCGAGA GTCCGGAGGA GGTCGTGGTG GTGTGGGACA ACGGCACGGC CGCCAACTAC 
CGCTGCTCAG GGGCCTACGA CCTGCGTATC CTGGACAGCG CGCCCACCGG CATCAAGCAT 
GATGGAACTA TGTGTGATAC CTGCCGCCAG CAACCAATCA TTGGCATTCG ATGGAAATGT 
GCAGAGTGCA CCAATTATGA TTTGTGCACT GTTTGTTATC ATGGAGATAA ACATCACTTA 
AGGCATCGCT TTTATAGGAT TACTACACCA GGAAGTGAAC GGGTTCTGTT AGAGTCTCGT 
AGAAAATCTA AGAAGATTAC AGCCAGAGGA ATCTTTGCTG GTGCCAGAGT GGTGCGAGGA 
GTGGACTGGC AGTGGGAAGA TCAAGATGGA GGCAATGGGC GTAGAGGAAA GGTCACGGAG 
ATCCAGGACT GGAGTGCATC CAGCCCACAC AGTGCAGCCT ATGTTCTCTG GGATAACGGT 
GCTAAGAACC TTTACAGGGT TGGCTTTGAG GGCATGTCTG ATCTCAAGTG TGTCCAGGAT 
GCCAAAGGAG GTTCTTTCTA CAGAGATCAT TGCCCTGTGC TAGGTGAGCA GAATGGCAAC 
AGGAATCCTG GTGGATTGCA GATTGGTGAC CTGGTAAATA TAGACCTTGA TCTAGAAATT 
GTCCAGTCTT TACAGCACGG TCATGGAGGA TGGACCGATG GCATGTTTGA GACTTTAACT 
ACAACTGGAA CCGTCTGTGG CATTGATGAA GATCATGACA TTGTAGTACA GTATCCAAGT 
GGCAATAGAT GGACTTTCAA TCCTGCTGTT CTCACTAAGG CAAACATTGT CCGAAGTGGG 
GATGCTGCAC AGGGTGCAGA AGGAGGCACC TCACAGTTTC AAGTGGGTGA TCTTGTGCAA 
GTTTGTTATG ATCTGGAAAG AATTAAACTT CTCCAAAGAG GACATGGTGA ATGGGCTGAA 
GCAATGCTTC CAACTTTAGG TAAAGTTGGC CGAGTACAAC AGATTTATTC GGACAGTGAC 
TTAAAGGTGG AAGTCTGTGG AACATCTTGG ACGTATAACC CTGCAGCAGT TTCCAAGGTG 
GCCCCTGCAG GATCAGCCAT TAGCAATGCA TCTGGTGAAA GACTCTCCCA GCTTCTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGTGAC CTCAATGAAG AGCTGGTTAA GGCTGCTGCC 
AATGGAGACG TGGCTAAAGT GGAAGATTTG CTGAAAAGAC CAGATGTGGA TGTAAACGGA 
CAGTGTGCTG GCCACACAGC AATGCAAGCT GCTAGTCAGA ACGGACATGT TGATATTTTG 
AAATTACTTT TGAAGCAAAA TGTTGATGTT GAAGCAGAGG ATAAAGATGG AGATCGAGCC 
GTTCACCATG CAGCCTTTGG AGATGAGGGT GCTGTCATAG AAGTCCTACA CCGAGGCAGT 
GCTGACCTGA ATGCTCGTAA TAAGCGTAGA CAGACGCCTC TTCATATTGC TGTCAATAAA 
GGTCATCTTC AAGTTGTGAA GACTTTGTTG GATTTTGGTT GTCATCCCAG TCTCCAGGAT 
TCTGAAGGTG ACACCCCTCT CCATGATGCC ATAAGTAAGA AACGGGATGA CATTCTGGCA 
GTTCTTCTGG AAGCTGGAGC AGATGTGACC ATCACGAACA ATAACGGGTT CAATGCCCTG 
CACCATGCTG CACTGAGAGG GAACCCCAGT GCAATGCGTG TTTTACTCTC AAAATTACCA 
AGACCATGGA TTGTGGATGA GAAGAAAGAT GATGGTTATA CTGCCTTGCA CCTGGCTGCC 
CTGAATAATC ATGTGGAAGT GGCTGAGCTG TTGGTGCATC AGGGAAATGC AAACCTGGAT 
ATCCAGAATG TGAACCAGCA AACAGCCCTA CACCTTGCTG TTGAACGGCA ACACACCCAG 
ATTGTTAGGC TTTTGGTCCG TGCAGGTGCT AAGCTAGACA TTCAGGATAA GGATGGAGAT 
ACTCCTCTGC ATGAAGCTTT GAGACATCAT ACTTTGTCTC AACTACGTCA GCTACAAGAC 
ATGCAAGATG TGGGCAAGGT AGACGCTGCC TGGGAACCTT CAAAAAACAC ATTAATAATG 
GGACTTGGTA CCCAGGGGGC TGAGAAGAAG AGTGCAGCAT CCATTGCGTG TTTCTTGGCA 
GCCAATGGTG CTGATCTCAG CATTCGAAAT AAGAAGGGTC AGTCACCACT GGATCTCTGT 
CCTGACCCAA GTCTCTGCAA AGCTTTGGCA AAATGTCATA AGGAAAAAGT CAGTGGCCAA 
GTGGGTTCTC GGAGTCCTTC TATGATTAGC AACGATTCTG AAACTTTAGA GGAATGTATG 
GTGTGCTCAG ACATGAAGAG AGATACTCTT TTTGGTCCGT GTGGACACAT TGCAACCTGT 
TCTTTGTGTT CCCCTCGTGT CAAGAAATGC CTCATTTGTA AAGAGCAGGT TCAGTCCAGG 
ACAAAGATTG AAGAATGTGT GGTGTGCTCC GACAAGAAAG CAGCTGTTCT CTTTCAACCT 
TGTGGGCACA TGTGTGCTTG TGAGAACTGT GCCAGCCTGA TGAAAAAGTG TGTGCAGTGT 
CGGGCAGTGG TTGAGCGGAG GGTGCCTTTC ATCACGTGCT GTGGTGGCAA GAGCTCAGAA 
GACCCCTCTG ATGAAATCTC AAGTGGGAAT ATCCCAGTGC TGCAGAAGGA CAAAGACAAC 
ACCAATGTTA ACGCAGATGT GCAGAAACTG CAGCAGCAGC TTCAGGACAT TAAGGAGCAG 
ACCATGTGCC CTGTGTGCTT GGATCGCCTG AAGAACATGA TTTTCCTCTG TGGCCATGGA 
ACATGCCAGC TCTGTGGAGA CCGGATGAGT GAATGCCCAA TTTGTCGCAA GGCAATTGAA 
CGAAGGATTC TTTTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib1 ( NM_144860.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_659109.2 Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), mRNA.

Price & Availability↑

CloneID	OMu18519
Clone ID Related Accession (Same CDS sequence)	XM_006525811.4 , NM_144860.3 , NM_001364997.1 , NM_144860.2 , XM_006525811.3 , XM_006525810.2
Accession Version	NM_144860.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY245539.1. On or before May 4, 2006 this sequence version replaced XM_976932.1, NM_144860.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY245539.1, AY147848.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAGTAACT CCCGCAACAA CCGAGTGATG GTGGAGGGGG TCGGCGCCCG CGTAGTGCGC 
GGCCCGGACT GGAAGTGGGG CAAGCAGGAC GGCGGCGAGG GCCATGTAGG CACTGTACGG 
AGCTTCGAGA GTCCGGAGGA GGTCGTGGTG GTGTGGGACA ACGGCACGGC CGCCAACTAC 
CGCTGCTCAG GGGCCTACGA CCTGCGTATC CTGGACAGCG CGCCCACCGG CATCAAGCAT 
GATGGAACTA TGTGTGATAC CTGCCGCCAG CAACCAATCA TTGGCATTCG ATGGAAATGT 
GCAGAGTGCA CCAATTATGA TTTGTGCACT GTTTGTTATC ATGGAGATAA ACATCACTTA 
AGGCATCGCT TTTATAGGAT TACTACACCA GGAAGTGAAC GGGTTCTGTT AGAGTCTCGT 
AGAAAATCTA AGAAGATTAC AGCCAGAGGA ATCTTTGCTG GTGCCAGAGT GGTGCGAGGA 
GTGGACTGGC AGTGGGAAGA TCAAGATGGA GGCAATGGGC GTAGAGGAAA GGTCACGGAG 
ATCCAGGACT GGAGTGCATC CAGCCCACAC AGTGCAGCCT ATGTTCTCTG GGATAACGGT 
GCTAAGAACC TTTACAGGGT TGGCTTTGAG GGCATGTCTG ATCTCAAGTG TGTCCAGGAT 
GCCAAAGGAG GTTCTTTCTA CAGAGATCAT TGCCCTGTGC TAGGTGAGCA GAATGGCAAC 
AGGAATCCTG GTGGATTGCA GATTGGTGAC CTGGTAAATA TAGACCTTGA TCTAGAAATT 
GTCCAGTCTT TACAGCACGG TCATGGAGGA TGGACCGATG GCATGTTTGA GACTTTAACT 
ACAACTGGAA CCGTCTGTGG CATTGATGAA GATCATGACA TTGTAGTACA GTATCCAAGT 
GGCAATAGAT GGACTTTCAA TCCTGCTGTT CTCACTAAGG CAAACATTGT CCGAAGTGGG 
GATGCTGCAC AGGGTGCAGA AGGAGGCACC TCACAGTTTC AAGTGGGTGA TCTTGTGCAA 
GTTTGTTATG ATCTGGAAAG AATTAAACTT CTCCAAAGAG GACATGGTGA ATGGGCTGAA 
GCAATGCTTC CAACTTTAGG TAAAGTTGGC CGAGTACAAC AGATTTATTC GGACAGTGAC 
TTAAAGGTGG AAGTCTGTGG AACATCTTGG ACGTATAACC CTGCAGCAGT TTCCAAGGTG 
GCCCCTGCAG GATCAGCCAT TAGCAATGCA TCTGGTGAAA GACTCTCCCA GCTTCTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGTGAC CTCAATGAAG AGCTGGTTAA GGCTGCTGCC 
AATGGAGACG TGGCTAAAGT GGAAGATTTG CTGAAAAGAC CAGATGTGGA TGTAAACGGA 
CAGTGTGCTG GCCACACAGC AATGCAAGCT GCTAGTCAGA ACGGACATGT TGATATTTTG 
AAATTACTTT TGAAGCAAAA TGTTGATGTT GAAGCAGAGG ATAAAGATGG AGATCGAGCC 
GTTCACCATG CAGCCTTTGG AGATGAGGGT GCTGTCATAG AAGTCCTACA CCGAGGCAGT 
GCTGACCTGA ATGCTCGTAA TAAGCGTAGA CAGACGCCTC TTCATATTGC TGTCAATAAA 
GGTCATCTTC AAGTTGTGAA GACTTTGTTG GATTTTGGTT GTCATCCCAG TCTCCAGGAT 
TCTGAAGGTG ACACCCCTCT CCATGATGCC ATAAGTAAGA AACGGGATGA CATTCTGGCA 
GTTCTTCTGG AAGCTGGAGC AGATGTGACC ATCACGAACA ATAACGGGTT CAATGCCCTG 
CACCATGCTG CACTGAGAGG GAACCCCAGT GCAATGCGTG TTTTACTCTC AAAATTACCA 
AGACCATGGA TTGTGGATGA GAAGAAAGAT GATGGTTATA CTGCCTTGCA CCTGGCTGCC 
CTGAATAATC ATGTGGAAGT GGCTGAGCTG TTGGTGCATC AGGGAAATGC AAACCTGGAT 
ATCCAGAATG TGAACCAGCA AACAGCCCTA CACCTTGCTG TTGAACGGCA ACACACCCAG 
ATTGTTAGGC TTTTGGTCCG TGCAGGTGCT AAGCTAGACA TTCAGGATAA GGATGGAGAT 
ACTCCTCTGC ATGAAGCTTT GAGACATCAT ACTTTGTCTC AACTACGTCA GCTACAAGAC 
ATGCAAGATG TGGGCAAGGT AGACGCTGCC TGGGAACCTT CAAAAAACAC ATTAATAATG 
GGACTTGGTA CCCAGGGGGC TGAGAAGAAG AGTGCAGCAT CCATTGCGTG TTTCTTGGCA 
GCCAATGGTG CTGATCTCAG CATTCGAAAT AAGAAGGGTC AGTCACCACT GGATCTCTGT 
CCTGACCCAA GTCTCTGCAA AGCTTTGGCA AAATGTCATA AGGAAAAAGT CAGTGGCCAA 
GTGGGTTCTC GGAGTCCTTC TATGATTAGC AACGATTCTG AAACTTTAGA GGAATGTATG 
GTGTGCTCAG ACATGAAGAG AGATACTCTT TTTGGTCCGT GTGGACACAT TGCAACCTGT 
TCTTTGTGTT CCCCTCGTGT CAAGAAATGC CTCATTTGTA AAGAGCAGGT TCAGTCCAGG 
ACAAAGATTG AAGAATGTGT GGTGTGCTCC GACAAGAAAG CAGCTGTTCT CTTTCAACCT 
TGTGGGCACA TGTGTGCTTG TGAGAACTGT GCCAGCCTGA TGAAAAAGTG TGTGCAGTGT 
CGGGCAGTGG TTGAGCGGAG GGTGCCTTTC ATCACGTGCT GTGGTGGCAA GAGCTCAGAA 
GACCCCTCTG ATGAAATCTC AAGTGGGAAT ATCCCAGTGC TGCAGAAGGA CAAAGACAAC 
ACCAATGTTA ACGCAGATGT GCAGAAACTG CAGCAGCAGC TTCAGGACAT TAAGGAGCAG 
ACCATGTGCC CTGTGTGCTT GGATCGCCTG AAGAACATGA TTTTCCTCTG TGGCCATGGA 
ACATGCCAGC TCTGTGGAGA CCGGATGAGT GAATGCCCAA TTTGTCGCAA GGCAATTGAA 
CGAAGGATTC TTTTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659109.2
CDS	681..3701
Translation	MSNSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFITCCGGKSSEDPSDEISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	NM_144860.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144860.2

ORF Insert Sequence:

ATGAGTAACT CCCGCAACAA CCGAGTGATG GTGGAGGGGG TCGGCGCCCG CGTAGTGCGC 
GGCCCGGACT GGAAGTGGGG CAAGCAGGAC GGCGGCGAGG GCCATGTAGG CACTGTACGG 
AGCTTCGAGA GTCCGGAGGA GGTCGTGGTG GTGTGGGACA ACGGCACGGC CGCCAACTAC 
CGCTGCTCAG GGGCCTACGA CCTGCGTATC CTGGACAGCG CGCCCACCGG CATCAAGCAT 
GATGGAACTA TGTGTGATAC CTGCCGCCAG CAACCAATCA TTGGCATTCG ATGGAAATGT 
GCAGAGTGCA CCAATTATGA TTTGTGCACT GTTTGTTATC ATGGAGATAA ACATCACTTA 
AGGCATCGCT TTTATAGGAT TACTACACCA GGAAGTGAAC GGGTTCTGTT AGAGTCTCGT 
AGAAAATCTA AGAAGATTAC AGCCAGAGGA ATCTTTGCTG GTGCCAGAGT GGTGCGAGGA 
GTGGACTGGC AGTGGGAAGA TCAAGATGGA GGCAATGGGC GTAGAGGAAA GGTCACGGAG 
ATCCAGGACT GGAGTGCATC CAGCCCACAC AGTGCAGCCT ATGTTCTCTG GGATAACGGT 
GCTAAGAACC TTTACAGGGT TGGCTTTGAG GGCATGTCTG ATCTCAAGTG TGTCCAGGAT 
GCCAAAGGAG GTTCTTTCTA CAGAGATCAT TGCCCTGTGC TAGGTGAGCA GAATGGCAAC 
AGGAATCCTG GTGGATTGCA GATTGGTGAC CTGGTAAATA TAGACCTTGA TCTAGAAATT 
GTCCAGTCTT TACAGCACGG TCATGGAGGA TGGACCGATG GCATGTTTGA GACTTTAACT 
ACAACTGGAA CCGTCTGTGG CATTGATGAA GATCATGACA TTGTAGTACA GTATCCAAGT 
GGCAATAGAT GGACTTTCAA TCCTGCTGTT CTCACTAAGG CAAACATTGT CCGAAGTGGG 
GATGCTGCAC AGGGTGCAGA AGGAGGCACC TCACAGTTTC AAGTGGGTGA TCTTGTGCAA 
GTTTGTTATG ATCTGGAAAG AATTAAACTT CTCCAAAGAG GACATGGTGA ATGGGCTGAA 
GCAATGCTTC CAACTTTAGG TAAAGTTGGC CGAGTACAAC AGATTTATTC GGACAGTGAC 
TTAAAGGTGG AAGTCTGTGG AACATCTTGG ACGTATAACC CTGCAGCAGT TTCCAAGGTG 
GCCCCTGCAG GATCAGCCAT TAGCAATGCA TCTGGTGAAA GACTCTCCCA GCTTCTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGTGAC CTCAATGAAG AGCTGGTTAA GGCTGCTGCC 
AATGGAGACG TGGCTAAAGT GGAAGATTTG CTGAAAAGAC CAGATGTGGA TGTAAACGGA 
CAGTGTGCTG GCCACACAGC AATGCAAGCT GCTAGTCAGA ACGGACATGT TGATATTTTG 
AAATTACTTT TGAAGCAAAA TGTTGATGTT GAAGCAGAGG ATAAAGATGG AGATCGAGCC 
GTTCACCATG CAGCCTTTGG AGATGAGGGT GCTGTCATAG AAGTCCTACA CCGAGGCAGT 
GCTGACCTGA ATGCTCGTAA TAAGCGTAGA CAGACGCCTC TTCATATTGC TGTCAATAAA 
GGTCATCTTC AAGTTGTGAA GACTTTGTTG GATTTTGGTT GTCATCCCAG TCTCCAGGAT 
TCTGAAGGTG ACACCCCTCT CCATGATGCC ATAAGTAAGA AACGGGATGA CATTCTGGCA 
GTTCTTCTGG AAGCTGGAGC AGATGTGACC ATCACGAACA ATAACGGGTT CAATGCCCTG 
CACCATGCTG CACTGAGAGG GAACCCCAGT GCAATGCGTG TTTTACTCTC AAAATTACCA 
AGACCATGGA TTGTGGATGA GAAGAAAGAT GATGGTTATA CTGCCTTGCA CCTGGCTGCC 
CTGAATAATC ATGTGGAAGT GGCTGAGCTG TTGGTGCATC AGGGAAATGC AAACCTGGAT 
ATCCAGAATG TGAACCAGCA AACAGCCCTA CACCTTGCTG TTGAACGGCA ACACACCCAG 
ATTGTTAGGC TTTTGGTCCG TGCAGGTGCT AAGCTAGACA TTCAGGATAA GGATGGAGAT 
ACTCCTCTGC ATGAAGCTTT GAGACATCAT ACTTTGTCTC AACTACGTCA GCTACAAGAC 
ATGCAAGATG TGGGCAAGGT AGACGCTGCC TGGGAACCTT CAAAAAACAC ATTAATAATG 
GGACTTGGTA CCCAGGGGGC TGAGAAGAAG AGTGCAGCAT CCATTGCGTG TTTCTTGGCA 
GCCAATGGTG CTGATCTCAG CATTCGAAAT AAGAAGGGTC AGTCACCACT GGATCTCTGT 
CCTGACCCAA GTCTCTGCAA AGCTTTGGCA AAATGTCATA AGGAAAAAGT CAGTGGCCAA 
GTGGGTTCTC GGAGTCCTTC TATGATTAGC AACGATTCTG AAACTTTAGA GGAATGTATG 
GTGTGCTCAG ACATGAAGAG AGATACTCTT TTTGGTCCGT GTGGACACAT TGCAACCTGT 
TCTTTGTGTT CCCCTCGTGT CAAGAAATGC CTCATTTGTA AAGAGCAGGT TCAGTCCAGG 
ACAAAGATTG AAGAATGTGT GGTGTGCTCC GACAAGAAAG CAGCTGTTCT CTTTCAACCT 
TGTGGGCACA TGTGTGCTTG TGAGAACTGT GCCAGCCTGA TGAAAAAGTG TGTGCAGTGT 
CGGGCAGTGG TTGAGCGGAG GGTGCCTTTC ATCACGTGCT GTGGTGGCAA GAGCTCAGAA 
GACCCCTCTG ATGAAATCTC AAGTGGGAAT ATCCCAGTGC TGCAGAAGGA CAAAGACAAC 
ACCAATGTTA ACGCAGATGT GCAGAAACTG CAGCAGCAGC TTCAGGACAT TAAGGAGCAG 
ACCATGTGCC CTGTGTGCTT GGATCGCCTG AAGAACATGA TTTTCCTCTG TGGCCATGGA 
ACATGCCAGC TCTGTGGAGA CCGGATGAGT GAATGCCCAA TTTGTCGCAA GGCAATTGAA 
CGAAGGATTC TTTTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib1 ( XM_006525811.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525874.1 Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu18519
Clone ID Related Accession (Same CDS sequence)	XM_006525811.4 , NM_144860.3 , NM_001364997.1 , NM_144860.2 , XM_006525811.3 , XM_006525810.2
Accession Version	XM_006525811.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525811.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAACT CCCGCAACAA CCGAGTGATG GTGGAGGGGG TCGGCGCCCG CGTAGTGCGC 
GGCCCGGACT GGAAGTGGGG CAAGCAGGAC GGCGGCGAGG GCCATGTAGG CACTGTACGG 
AGCTTCGAGA GTCCGGAGGA GGTCGTGGTG GTGTGGGACA ACGGCACGGC CGCCAACTAC 
CGCTGCTCAG GGGCCTACGA CCTGCGTATC CTGGACAGCG CGCCCACCGG CATCAAGCAT 
GATGGAACTA TGTGTGATAC CTGCCGCCAG CAACCAATCA TTGGCATTCG ATGGAAATGT 
GCAGAGTGCA CCAATTATGA TTTGTGCACT GTTTGTTATC ATGGAGATAA ACATCACTTA 
AGGCATCGCT TTTATAGGAT TACTACACCA GGAAGTGAAC GGGTTCTGTT AGAGTCTCGT 
AGAAAATCTA AGAAGATTAC AGCCAGAGGA ATCTTTGCTG GTGCCAGAGT GGTGCGAGGA 
GTGGACTGGC AGTGGGAAGA TCAAGATGGA GGCAATGGGC GTAGAGGAAA GGTCACGGAG 
ATCCAGGACT GGAGTGCATC CAGCCCACAC AGTGCAGCCT ATGTTCTCTG GGATAACGGT 
GCTAAGAACC TTTACAGGGT TGGCTTTGAG GGCATGTCTG ATCTCAAGTG TGTCCAGGAT 
GCCAAAGGAG GTTCTTTCTA CAGAGATCAT TGCCCTGTGC TAGGTGAGCA GAATGGCAAC 
AGGAATCCTG GTGGATTGCA GATTGGTGAC CTGGTAAATA TAGACCTTGA TCTAGAAATT 
GTCCAGTCTT TACAGCACGG TCATGGAGGA TGGACCGATG GCATGTTTGA GACTTTAACT 
ACAACTGGAA CCGTCTGTGG CATTGATGAA GATCATGACA TTGTAGTACA GTATCCAAGT 
GGCAATAGAT GGACTTTCAA TCCTGCTGTT CTCACTAAGG CAAACATTGT CCGAAGTGGG 
GATGCTGCAC AGGGTGCAGA AGGAGGCACC TCACAGTTTC AAGTGGGTGA TCTTGTGCAA 
GTTTGTTATG ATCTGGAAAG AATTAAACTT CTCCAAAGAG GACATGGTGA ATGGGCTGAA 
GCAATGCTTC CAACTTTAGG TAAAGTTGGC CGAGTACAAC AGATTTATTC GGACAGTGAC 
TTAAAGGTGG AAGTCTGTGG AACATCTTGG ACGTATAACC CTGCAGCAGT TTCCAAGGTG 
GCCCCTGCAG GATCAGCCAT TAGCAATGCA TCTGGTGAAA GACTCTCCCA GCTTCTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGTGAC CTCAATGAAG AGCTGGTTAA GGCTGCTGCC 
AATGGAGACG TGGCTAAAGT GGAAGATTTG CTGAAAAGAC CAGATGTGGA TGTAAACGGA 
CAGTGTGCTG GCCACACAGC AATGCAAGCT GCTAGTCAGA ACGGACATGT TGATATTTTG 
AAATTACTTT TGAAGCAAAA TGTTGATGTT GAAGCAGAGG ATAAAGATGG AGATCGAGCC 
GTTCACCATG CAGCCTTTGG AGATGAGGGT GCTGTCATAG AAGTCCTACA CCGAGGCAGT 
GCTGACCTGA ATGCTCGTAA TAAGCGTAGA CAGACGCCTC TTCATATTGC TGTCAATAAA 
GGTCATCTTC AAGTTGTGAA GACTTTGTTG GATTTTGGTT GTCATCCCAG TCTCCAGGAT 
TCTGAAGGTG ACACCCCTCT CCATGATGCC ATAAGTAAGA AACGGGATGA CATTCTGGCA 
GTTCTTCTGG AAGCTGGAGC AGATGTGACC ATCACGAACA ATAACGGGTT CAATGCCCTG 
CACCATGCTG CACTGAGAGG GAACCCCAGT GCAATGCGTG TTTTACTCTC AAAATTACCA 
AGACCATGGA TTGTGGATGA GAAGAAAGAT GATGGTTATA CTGCCTTGCA CCTGGCTGCC 
CTGAATAATC ATGTGGAAGT GGCTGAGCTG TTGGTGCATC AGGGAAATGC AAACCTGGAT 
ATCCAGAATG TGAACCAGCA AACAGCCCTA CACCTTGCTG TTGAACGGCA ACACACCCAG 
ATTGTTAGGC TTTTGGTCCG TGCAGGTGCT AAGCTAGACA TTCAGGATAA GGATGGAGAT 
ACTCCTCTGC ATGAAGCTTT GAGACATCAT ACTTTGTCTC AACTACGTCA GCTACAAGAC 
ATGCAAGATG TGGGCAAGGT AGACGCTGCC TGGGAACCTT CAAAAAACAC ATTAATAATG 
GGACTTGGTA CCCAGGGGGC TGAGAAGAAG AGTGCAGCAT CCATTGCGTG TTTCTTGGCA 
GCCAATGGTG CTGATCTCAG CATTCGAAAT AAGAAGGGTC AGTCACCACT GGATCTCTGT 
CCTGACCCAA GTCTCTGCAA AGCTTTGGCA AAATGTCATA AGGAAAAAGT CAGTGGCCAA 
GTGGGTTCTC GGAGTCCTTC TATGATTAGC AACGATTCTG AAACTTTAGA GGAATGTATG 
GTGTGCTCAG ACATGAAGAG AGATACTCTT TTTGGTCCGT GTGGACACAT TGCAACCTGT 
TCTTTGTGTT CCCCTCGTGT CAAGAAATGC CTCATTTGTA AAGAGCAGGT TCAGTCCAGG 
ACAAAGATTG AAGAATGTGT GGTGTGCTCC GACAAGAAAG CAGCTGTTCT CTTTCAACCT 
TGTGGGCACA TGTGTGCTTG TGAGAACTGT GCCAGCCTGA TGAAAAAGTG TGTGCAGTGT 
CGGGCAGTGG TTGAGCGGAG GGTGCCTTTC ATCACGTGCT GTGGTGGCAA GAGCTCAGAA 
GACCCCTCTG ATGAAATCTC AAGTGGGAAT ATCCCAGTGC TGCAGAAGGA CAAAGACAAC 
ACCAATGTTA ACGCAGATGT GCAGAAACTG CAGCAGCAGC TTCAGGACAT TAAGGAGCAG 
ACCATGTGCC CTGTGTGCTT GGATCGCCTG AAGAACATGA TTTTCCTCTG TGGCCATGGA 
ACATGCCAGC TCTGTGGAGA CCGGATGAGT GAATGCCCAA TTTGTCGCAA GGCAATTGAA 
CGAAGGATTC TTTTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525874.1
CDS	174..3194
Translation	MSNSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFITCCGGKSSEDPSDEISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_006525811.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525811.4

ORF Insert Sequence:

ATGAGTAACT CCCGCAACAA CCGAGTGATG GTGGAGGGGG TCGGCGCCCG CGTAGTGCGC 
GGCCCGGACT GGAAGTGGGG CAAGCAGGAC GGCGGCGAGG GCCATGTAGG CACTGTACGG 
AGCTTCGAGA GTCCGGAGGA GGTCGTGGTG GTGTGGGACA ACGGCACGGC CGCCAACTAC 
CGCTGCTCAG GGGCCTACGA CCTGCGTATC CTGGACAGCG CGCCCACCGG CATCAAGCAT 
GATGGAACTA TGTGTGATAC CTGCCGCCAG CAACCAATCA TTGGCATTCG ATGGAAATGT 
GCAGAGTGCA CCAATTATGA TTTGTGCACT GTTTGTTATC ATGGAGATAA ACATCACTTA 
AGGCATCGCT TTTATAGGAT TACTACACCA GGAAGTGAAC GGGTTCTGTT AGAGTCTCGT 
AGAAAATCTA AGAAGATTAC AGCCAGAGGA ATCTTTGCTG GTGCCAGAGT GGTGCGAGGA 
GTGGACTGGC AGTGGGAAGA TCAAGATGGA GGCAATGGGC GTAGAGGAAA GGTCACGGAG 
ATCCAGGACT GGAGTGCATC CAGCCCACAC AGTGCAGCCT ATGTTCTCTG GGATAACGGT 
GCTAAGAACC TTTACAGGGT TGGCTTTGAG GGCATGTCTG ATCTCAAGTG TGTCCAGGAT 
GCCAAAGGAG GTTCTTTCTA CAGAGATCAT TGCCCTGTGC TAGGTGAGCA GAATGGCAAC 
AGGAATCCTG GTGGATTGCA GATTGGTGAC CTGGTAAATA TAGACCTTGA TCTAGAAATT 
GTCCAGTCTT TACAGCACGG TCATGGAGGA TGGACCGATG GCATGTTTGA GACTTTAACT 
ACAACTGGAA CCGTCTGTGG CATTGATGAA GATCATGACA TTGTAGTACA GTATCCAAGT 
GGCAATAGAT GGACTTTCAA TCCTGCTGTT CTCACTAAGG CAAACATTGT CCGAAGTGGG 
GATGCTGCAC AGGGTGCAGA AGGAGGCACC TCACAGTTTC AAGTGGGTGA TCTTGTGCAA 
GTTTGTTATG ATCTGGAAAG AATTAAACTT CTCCAAAGAG GACATGGTGA ATGGGCTGAA 
GCAATGCTTC CAACTTTAGG TAAAGTTGGC CGAGTACAAC AGATTTATTC GGACAGTGAC 
TTAAAGGTGG AAGTCTGTGG AACATCTTGG ACGTATAACC CTGCAGCAGT TTCCAAGGTG 
GCCCCTGCAG GATCAGCCAT TAGCAATGCA TCTGGTGAAA GACTCTCCCA GCTTCTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGTGAC CTCAATGAAG AGCTGGTTAA GGCTGCTGCC 
AATGGAGACG TGGCTAAAGT GGAAGATTTG CTGAAAAGAC CAGATGTGGA TGTAAACGGA 
CAGTGTGCTG GCCACACAGC AATGCAAGCT GCTAGTCAGA ACGGACATGT TGATATTTTG 
AAATTACTTT TGAAGCAAAA TGTTGATGTT GAAGCAGAGG ATAAAGATGG AGATCGAGCC 
GTTCACCATG CAGCCTTTGG AGATGAGGGT GCTGTCATAG AAGTCCTACA CCGAGGCAGT 
GCTGACCTGA ATGCTCGTAA TAAGCGTAGA CAGACGCCTC TTCATATTGC TGTCAATAAA 
GGTCATCTTC AAGTTGTGAA GACTTTGTTG GATTTTGGTT GTCATCCCAG TCTCCAGGAT 
TCTGAAGGTG ACACCCCTCT CCATGATGCC ATAAGTAAGA AACGGGATGA CATTCTGGCA 
GTTCTTCTGG AAGCTGGAGC AGATGTGACC ATCACGAACA ATAACGGGTT CAATGCCCTG 
CACCATGCTG CACTGAGAGG GAACCCCAGT GCAATGCGTG TTTTACTCTC AAAATTACCA 
AGACCATGGA TTGTGGATGA GAAGAAAGAT GATGGTTATA CTGCCTTGCA CCTGGCTGCC 
CTGAATAATC ATGTGGAAGT GGCTGAGCTG TTGGTGCATC AGGGAAATGC AAACCTGGAT 
ATCCAGAATG TGAACCAGCA AACAGCCCTA CACCTTGCTG TTGAACGGCA ACACACCCAG 
ATTGTTAGGC TTTTGGTCCG TGCAGGTGCT AAGCTAGACA TTCAGGATAA GGATGGAGAT 
ACTCCTCTGC ATGAAGCTTT GAGACATCAT ACTTTGTCTC AACTACGTCA GCTACAAGAC 
ATGCAAGATG TGGGCAAGGT AGACGCTGCC TGGGAACCTT CAAAAAACAC ATTAATAATG 
GGACTTGGTA CCCAGGGGGC TGAGAAGAAG AGTGCAGCAT CCATTGCGTG TTTCTTGGCA 
GCCAATGGTG CTGATCTCAG CATTCGAAAT AAGAAGGGTC AGTCACCACT GGATCTCTGT 
CCTGACCCAA GTCTCTGCAA AGCTTTGGCA AAATGTCATA AGGAAAAAGT CAGTGGCCAA 
GTGGGTTCTC GGAGTCCTTC TATGATTAGC AACGATTCTG AAACTTTAGA GGAATGTATG 
GTGTGCTCAG ACATGAAGAG AGATACTCTT TTTGGTCCGT GTGGACACAT TGCAACCTGT 
TCTTTGTGTT CCCCTCGTGT CAAGAAATGC CTCATTTGTA AAGAGCAGGT TCAGTCCAGG 
ACAAAGATTG AAGAATGTGT GGTGTGCTCC GACAAGAAAG CAGCTGTTCT CTTTCAACCT 
TGTGGGCACA TGTGTGCTTG TGAGAACTGT GCCAGCCTGA TGAAAAAGTG TGTGCAGTGT 
CGGGCAGTGG TTGAGCGGAG GGTGCCTTTC ATCACGTGCT GTGGTGGCAA GAGCTCAGAA 
GACCCCTCTG ATGAAATCTC AAGTGGGAAT ATCCCAGTGC TGCAGAAGGA CAAAGACAAC 
ACCAATGTTA ACGCAGATGT GCAGAAACTG CAGCAGCAGC TTCAGGACAT TAAGGAGCAG 
ACCATGTGCC CTGTGTGCTT GGATCGCCTG AAGAACATGA TTTTCCTCTG TGGCCATGGA 
ACATGCCAGC TCTGTGGAGA CCGGATGAGT GAATGCCCAA TTTGTCGCAA GGCAATTGAA 
CGAAGGATTC TTTTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib1 ( XM_006525811.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525874.1 Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu18519
Clone ID Related Accession (Same CDS sequence)	XM_006525811.4 , NM_144860.3 , NM_001364997.1 , NM_144860.2 , XM_006525811.3 , XM_006525810.2
Accession Version	XM_006525811.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525811.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAACT CCCGCAACAA CCGAGTGATG GTGGAGGGGG TCGGCGCCCG CGTAGTGCGC 
GGCCCGGACT GGAAGTGGGG CAAGCAGGAC GGCGGCGAGG GCCATGTAGG CACTGTACGG 
AGCTTCGAGA GTCCGGAGGA GGTCGTGGTG GTGTGGGACA ACGGCACGGC CGCCAACTAC 
CGCTGCTCAG GGGCCTACGA CCTGCGTATC CTGGACAGCG CGCCCACCGG CATCAAGCAT 
GATGGAACTA TGTGTGATAC CTGCCGCCAG CAACCAATCA TTGGCATTCG ATGGAAATGT 
GCAGAGTGCA CCAATTATGA TTTGTGCACT GTTTGTTATC ATGGAGATAA ACATCACTTA 
AGGCATCGCT TTTATAGGAT TACTACACCA GGAAGTGAAC GGGTTCTGTT AGAGTCTCGT 
AGAAAATCTA AGAAGATTAC AGCCAGAGGA ATCTTTGCTG GTGCCAGAGT GGTGCGAGGA 
GTGGACTGGC AGTGGGAAGA TCAAGATGGA GGCAATGGGC GTAGAGGAAA GGTCACGGAG 
ATCCAGGACT GGAGTGCATC CAGCCCACAC AGTGCAGCCT ATGTTCTCTG GGATAACGGT 
GCTAAGAACC TTTACAGGGT TGGCTTTGAG GGCATGTCTG ATCTCAAGTG TGTCCAGGAT 
GCCAAAGGAG GTTCTTTCTA CAGAGATCAT TGCCCTGTGC TAGGTGAGCA GAATGGCAAC 
AGGAATCCTG GTGGATTGCA GATTGGTGAC CTGGTAAATA TAGACCTTGA TCTAGAAATT 
GTCCAGTCTT TACAGCACGG TCATGGAGGA TGGACCGATG GCATGTTTGA GACTTTAACT 
ACAACTGGAA CCGTCTGTGG CATTGATGAA GATCATGACA TTGTAGTACA GTATCCAAGT 
GGCAATAGAT GGACTTTCAA TCCTGCTGTT CTCACTAAGG CAAACATTGT CCGAAGTGGG 
GATGCTGCAC AGGGTGCAGA AGGAGGCACC TCACAGTTTC AAGTGGGTGA TCTTGTGCAA 
GTTTGTTATG ATCTGGAAAG AATTAAACTT CTCCAAAGAG GACATGGTGA ATGGGCTGAA 
GCAATGCTTC CAACTTTAGG TAAAGTTGGC CGAGTACAAC AGATTTATTC GGACAGTGAC 
TTAAAGGTGG AAGTCTGTGG AACATCTTGG ACGTATAACC CTGCAGCAGT TTCCAAGGTG 
GCCCCTGCAG GATCAGCCAT TAGCAATGCA TCTGGTGAAA GACTCTCCCA GCTTCTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGTGAC CTCAATGAAG AGCTGGTTAA GGCTGCTGCC 
AATGGAGACG TGGCTAAAGT GGAAGATTTG CTGAAAAGAC CAGATGTGGA TGTAAACGGA 
CAGTGTGCTG GCCACACAGC AATGCAAGCT GCTAGTCAGA ACGGACATGT TGATATTTTG 
AAATTACTTT TGAAGCAAAA TGTTGATGTT GAAGCAGAGG ATAAAGATGG AGATCGAGCC 
GTTCACCATG CAGCCTTTGG AGATGAGGGT GCTGTCATAG AAGTCCTACA CCGAGGCAGT 
GCTGACCTGA ATGCTCGTAA TAAGCGTAGA CAGACGCCTC TTCATATTGC TGTCAATAAA 
GGTCATCTTC AAGTTGTGAA GACTTTGTTG GATTTTGGTT GTCATCCCAG TCTCCAGGAT 
TCTGAAGGTG ACACCCCTCT CCATGATGCC ATAAGTAAGA AACGGGATGA CATTCTGGCA 
GTTCTTCTGG AAGCTGGAGC AGATGTGACC ATCACGAACA ATAACGGGTT CAATGCCCTG 
CACCATGCTG CACTGAGAGG GAACCCCAGT GCAATGCGTG TTTTACTCTC AAAATTACCA 
AGACCATGGA TTGTGGATGA GAAGAAAGAT GATGGTTATA CTGCCTTGCA CCTGGCTGCC 
CTGAATAATC ATGTGGAAGT GGCTGAGCTG TTGGTGCATC AGGGAAATGC AAACCTGGAT 
ATCCAGAATG TGAACCAGCA AACAGCCCTA CACCTTGCTG TTGAACGGCA ACACACCCAG 
ATTGTTAGGC TTTTGGTCCG TGCAGGTGCT AAGCTAGACA TTCAGGATAA GGATGGAGAT 
ACTCCTCTGC ATGAAGCTTT GAGACATCAT ACTTTGTCTC AACTACGTCA GCTACAAGAC 
ATGCAAGATG TGGGCAAGGT AGACGCTGCC TGGGAACCTT CAAAAAACAC ATTAATAATG 
GGACTTGGTA CCCAGGGGGC TGAGAAGAAG AGTGCAGCAT CCATTGCGTG TTTCTTGGCA 
GCCAATGGTG CTGATCTCAG CATTCGAAAT AAGAAGGGTC AGTCACCACT GGATCTCTGT 
CCTGACCCAA GTCTCTGCAA AGCTTTGGCA AAATGTCATA AGGAAAAAGT CAGTGGCCAA 
GTGGGTTCTC GGAGTCCTTC TATGATTAGC AACGATTCTG AAACTTTAGA GGAATGTATG 
GTGTGCTCAG ACATGAAGAG AGATACTCTT TTTGGTCCGT GTGGACACAT TGCAACCTGT 
TCTTTGTGTT CCCCTCGTGT CAAGAAATGC CTCATTTGTA AAGAGCAGGT TCAGTCCAGG 
ACAAAGATTG AAGAATGTGT GGTGTGCTCC GACAAGAAAG CAGCTGTTCT CTTTCAACCT 
TGTGGGCACA TGTGTGCTTG TGAGAACTGT GCCAGCCTGA TGAAAAAGTG TGTGCAGTGT 
CGGGCAGTGG TTGAGCGGAG GGTGCCTTTC ATCACGTGCT GTGGTGGCAA GAGCTCAGAA 
GACCCCTCTG ATGAAATCTC AAGTGGGAAT ATCCCAGTGC TGCAGAAGGA CAAAGACAAC 
ACCAATGTTA ACGCAGATGT GCAGAAACTG CAGCAGCAGC TTCAGGACAT TAAGGAGCAG 
ACCATGTGCC CTGTGTGCTT GGATCGCCTG AAGAACATGA TTTTCCTCTG TGGCCATGGA 
ACATGCCAGC TCTGTGGAGA CCGGATGAGT GAATGCCCAA TTTGTCGCAA GGCAATTGAA 
CGAAGGATTC TTTTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525874.1
CDS	753..3773
Translation	MSNSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFITCCGGKSSEDPSDEISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_006525811.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525811.3

ORF Insert Sequence:

ATGAGTAACT CCCGCAACAA CCGAGTGATG GTGGAGGGGG TCGGCGCCCG CGTAGTGCGC 
GGCCCGGACT GGAAGTGGGG CAAGCAGGAC GGCGGCGAGG GCCATGTAGG CACTGTACGG 
AGCTTCGAGA GTCCGGAGGA GGTCGTGGTG GTGTGGGACA ACGGCACGGC CGCCAACTAC 
CGCTGCTCAG GGGCCTACGA CCTGCGTATC CTGGACAGCG CGCCCACCGG CATCAAGCAT 
GATGGAACTA TGTGTGATAC CTGCCGCCAG CAACCAATCA TTGGCATTCG ATGGAAATGT 
GCAGAGTGCA CCAATTATGA TTTGTGCACT GTTTGTTATC ATGGAGATAA ACATCACTTA 
AGGCATCGCT TTTATAGGAT TACTACACCA GGAAGTGAAC GGGTTCTGTT AGAGTCTCGT 
AGAAAATCTA AGAAGATTAC AGCCAGAGGA ATCTTTGCTG GTGCCAGAGT GGTGCGAGGA 
GTGGACTGGC AGTGGGAAGA TCAAGATGGA GGCAATGGGC GTAGAGGAAA GGTCACGGAG 
ATCCAGGACT GGAGTGCATC CAGCCCACAC AGTGCAGCCT ATGTTCTCTG GGATAACGGT 
GCTAAGAACC TTTACAGGGT TGGCTTTGAG GGCATGTCTG ATCTCAAGTG TGTCCAGGAT 
GCCAAAGGAG GTTCTTTCTA CAGAGATCAT TGCCCTGTGC TAGGTGAGCA GAATGGCAAC 
AGGAATCCTG GTGGATTGCA GATTGGTGAC CTGGTAAATA TAGACCTTGA TCTAGAAATT 
GTCCAGTCTT TACAGCACGG TCATGGAGGA TGGACCGATG GCATGTTTGA GACTTTAACT 
ACAACTGGAA CCGTCTGTGG CATTGATGAA GATCATGACA TTGTAGTACA GTATCCAAGT 
GGCAATAGAT GGACTTTCAA TCCTGCTGTT CTCACTAAGG CAAACATTGT CCGAAGTGGG 
GATGCTGCAC AGGGTGCAGA AGGAGGCACC TCACAGTTTC AAGTGGGTGA TCTTGTGCAA 
GTTTGTTATG ATCTGGAAAG AATTAAACTT CTCCAAAGAG GACATGGTGA ATGGGCTGAA 
GCAATGCTTC CAACTTTAGG TAAAGTTGGC CGAGTACAAC AGATTTATTC GGACAGTGAC 
TTAAAGGTGG AAGTCTGTGG AACATCTTGG ACGTATAACC CTGCAGCAGT TTCCAAGGTG 
GCCCCTGCAG GATCAGCCAT TAGCAATGCA TCTGGTGAAA GACTCTCCCA GCTTCTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGTGAC CTCAATGAAG AGCTGGTTAA GGCTGCTGCC 
AATGGAGACG TGGCTAAAGT GGAAGATTTG CTGAAAAGAC CAGATGTGGA TGTAAACGGA 
CAGTGTGCTG GCCACACAGC AATGCAAGCT GCTAGTCAGA ACGGACATGT TGATATTTTG 
AAATTACTTT TGAAGCAAAA TGTTGATGTT GAAGCAGAGG ATAAAGATGG AGATCGAGCC 
GTTCACCATG CAGCCTTTGG AGATGAGGGT GCTGTCATAG AAGTCCTACA CCGAGGCAGT 
GCTGACCTGA ATGCTCGTAA TAAGCGTAGA CAGACGCCTC TTCATATTGC TGTCAATAAA 
GGTCATCTTC AAGTTGTGAA GACTTTGTTG GATTTTGGTT GTCATCCCAG TCTCCAGGAT 
TCTGAAGGTG ACACCCCTCT CCATGATGCC ATAAGTAAGA AACGGGATGA CATTCTGGCA 
GTTCTTCTGG AAGCTGGAGC AGATGTGACC ATCACGAACA ATAACGGGTT CAATGCCCTG 
CACCATGCTG CACTGAGAGG GAACCCCAGT GCAATGCGTG TTTTACTCTC AAAATTACCA 
AGACCATGGA TTGTGGATGA GAAGAAAGAT GATGGTTATA CTGCCTTGCA CCTGGCTGCC 
CTGAATAATC ATGTGGAAGT GGCTGAGCTG TTGGTGCATC AGGGAAATGC AAACCTGGAT 
ATCCAGAATG TGAACCAGCA AACAGCCCTA CACCTTGCTG TTGAACGGCA ACACACCCAG 
ATTGTTAGGC TTTTGGTCCG TGCAGGTGCT AAGCTAGACA TTCAGGATAA GGATGGAGAT 
ACTCCTCTGC ATGAAGCTTT GAGACATCAT ACTTTGTCTC AACTACGTCA GCTACAAGAC 
ATGCAAGATG TGGGCAAGGT AGACGCTGCC TGGGAACCTT CAAAAAACAC ATTAATAATG 
GGACTTGGTA CCCAGGGGGC TGAGAAGAAG AGTGCAGCAT CCATTGCGTG TTTCTTGGCA 
GCCAATGGTG CTGATCTCAG CATTCGAAAT AAGAAGGGTC AGTCACCACT GGATCTCTGT 
CCTGACCCAA GTCTCTGCAA AGCTTTGGCA AAATGTCATA AGGAAAAAGT CAGTGGCCAA 
GTGGGTTCTC GGAGTCCTTC TATGATTAGC AACGATTCTG AAACTTTAGA GGAATGTATG 
GTGTGCTCAG ACATGAAGAG AGATACTCTT TTTGGTCCGT GTGGACACAT TGCAACCTGT 
TCTTTGTGTT CCCCTCGTGT CAAGAAATGC CTCATTTGTA AAGAGCAGGT TCAGTCCAGG 
ACAAAGATTG AAGAATGTGT GGTGTGCTCC GACAAGAAAG CAGCTGTTCT CTTTCAACCT 
TGTGGGCACA TGTGTGCTTG TGAGAACTGT GCCAGCCTGA TGAAAAAGTG TGTGCAGTGT 
CGGGCAGTGG TTGAGCGGAG GGTGCCTTTC ATCACGTGCT GTGGTGGCAA GAGCTCAGAA 
GACCCCTCTG ATGAAATCTC AAGTGGGAAT ATCCCAGTGC TGCAGAAGGA CAAAGACAAC 
ACCAATGTTA ACGCAGATGT GCAGAAACTG CAGCAGCAGC TTCAGGACAT TAAGGAGCAG 
ACCATGTGCC CTGTGTGCTT GGATCGCCTG AAGAACATGA TTTTCCTCTG TGGCCATGGA 
ACATGCCAGC TCTGTGGAGA CCGGATGAGT GAATGCCCAA TTTGTCGCAA GGCAATTGAA 
CGAAGGATTC TTTTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib1 ( XM_006525810.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525873.1 Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu18519
Clone ID Related Accession (Same CDS sequence)	XM_006525811.4 , NM_144860.3 , NM_001364997.1 , NM_144860.2 , XM_006525811.3 , XM_006525810.2
Accession Version	XM_006525810.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525810.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAACT CCCGCAACAA CCGAGTGATG GTGGAGGGGG TCGGCGCCCG CGTAGTGCGC 
GGCCCGGACT GGAAGTGGGG CAAGCAGGAC GGCGGCGAGG GCCATGTAGG CACTGTACGG 
AGCTTCGAGA GTCCGGAGGA GGTCGTGGTG GTGTGGGACA ACGGCACGGC CGCCAACTAC 
CGCTGCTCAG GGGCCTACGA CCTGCGTATC CTGGACAGCG CGCCCACCGG CATCAAGCAT 
GATGGAACTA TGTGTGATAC CTGCCGCCAG CAACCAATCA TTGGCATTCG ATGGAAATGT 
GCAGAGTGCA CCAATTATGA TTTGTGCACT GTTTGTTATC ATGGAGATAA ACATCACTTA 
AGGCATCGCT TTTATAGGAT TACTACACCA GGAAGTGAAC GGGTTCTGTT AGAGTCTCGT 
AGAAAATCTA AGAAGATTAC AGCCAGAGGA ATCTTTGCTG GTGCCAGAGT GGTGCGAGGA 
GTGGACTGGC AGTGGGAAGA TCAAGATGGA GGCAATGGGC GTAGAGGAAA GGTCACGGAG 
ATCCAGGACT GGAGTGCATC CAGCCCACAC AGTGCAGCCT ATGTTCTCTG GGATAACGGT 
GCTAAGAACC TTTACAGGGT TGGCTTTGAG GGCATGTCTG ATCTCAAGTG TGTCCAGGAT 
GCCAAAGGAG GTTCTTTCTA CAGAGATCAT TGCCCTGTGC TAGGTGAGCA GAATGGCAAC 
AGGAATCCTG GTGGATTGCA GATTGGTGAC CTGGTAAATA TAGACCTTGA TCTAGAAATT 
GTCCAGTCTT TACAGCACGG TCATGGAGGA TGGACCGATG GCATGTTTGA GACTTTAACT 
ACAACTGGAA CCGTCTGTGG CATTGATGAA GATCATGACA TTGTAGTACA GTATCCAAGT 
GGCAATAGAT GGACTTTCAA TCCTGCTGTT CTCACTAAGG CAAACATTGT CCGAAGTGGG 
GATGCTGCAC AGGGTGCAGA AGGAGGCACC TCACAGTTTC AAGTGGGTGA TCTTGTGCAA 
GTTTGTTATG ATCTGGAAAG AATTAAACTT CTCCAAAGAG GACATGGTGA ATGGGCTGAA 
GCAATGCTTC CAACTTTAGG TAAAGTTGGC CGAGTACAAC AGATTTATTC GGACAGTGAC 
TTAAAGGTGG AAGTCTGTGG AACATCTTGG ACGTATAACC CTGCAGCAGT TTCCAAGGTG 
GCCCCTGCAG GATCAGCCAT TAGCAATGCA TCTGGTGAAA GACTCTCCCA GCTTCTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGTGAC CTCAATGAAG AGCTGGTTAA GGCTGCTGCC 
AATGGAGACG TGGCTAAAGT GGAAGATTTG CTGAAAAGAC CAGATGTGGA TGTAAACGGA 
CAGTGTGCTG GCCACACAGC AATGCAAGCT GCTAGTCAGA ACGGACATGT TGATATTTTG 
AAATTACTTT TGAAGCAAAA TGTTGATGTT GAAGCAGAGG ATAAAGATGG AGATCGAGCC 
GTTCACCATG CAGCCTTTGG AGATGAGGGT GCTGTCATAG AAGTCCTACA CCGAGGCAGT 
GCTGACCTGA ATGCTCGTAA TAAGCGTAGA CAGACGCCTC TTCATATTGC TGTCAATAAA 
GGTCATCTTC AAGTTGTGAA GACTTTGTTG GATTTTGGTT GTCATCCCAG TCTCCAGGAT 
TCTGAAGGTG ACACCCCTCT CCATGATGCC ATAAGTAAGA AACGGGATGA CATTCTGGCA 
GTTCTTCTGG AAGCTGGAGC AGATGTGACC ATCACGAACA ATAACGGGTT CAATGCCCTG 
CACCATGCTG CACTGAGAGG GAACCCCAGT GCAATGCGTG TTTTACTCTC AAAATTACCA 
AGACCATGGA TTGTGGATGA GAAGAAAGAT GATGGTTATA CTGCCTTGCA CCTGGCTGCC 
CTGAATAATC ATGTGGAAGT GGCTGAGCTG TTGGTGCATC AGGGAAATGC AAACCTGGAT 
ATCCAGAATG TGAACCAGCA AACAGCCCTA CACCTTGCTG TTGAACGGCA ACACACCCAG 
ATTGTTAGGC TTTTGGTCCG TGCAGGTGCT AAGCTAGACA TTCAGGATAA GGATGGAGAT 
ACTCCTCTGC ATGAAGCTTT GAGACATCAT ACTTTGTCTC AACTACGTCA GCTACAAGAC 
ATGCAAGATG TGGGCAAGGT AGACGCTGCC TGGGAACCTT CAAAAAACAC ATTAATAATG 
GGACTTGGTA CCCAGGGGGC TGAGAAGAAG AGTGCAGCAT CCATTGCGTG TTTCTTGGCA 
GCCAATGGTG CTGATCTCAG CATTCGAAAT AAGAAGGGTC AGTCACCACT GGATCTCTGT 
CCTGACCCAA GTCTCTGCAA AGCTTTGGCA AAATGTCATA AGGAAAAAGT CAGTGGCCAA 
GTGGGTTCTC GGAGTCCTTC TATGATTAGC AACGATTCTG AAACTTTAGA GGAATGTATG 
GTGTGCTCAG ACATGAAGAG AGATACTCTT TTTGGTCCGT GTGGACACAT TGCAACCTGT 
TCTTTGTGTT CCCCTCGTGT CAAGAAATGC CTCATTTGTA AAGAGCAGGT TCAGTCCAGG 
ACAAAGATTG AAGAATGTGT GGTGTGCTCC GACAAGAAAG CAGCTGTTCT CTTTCAACCT 
TGTGGGCACA TGTGTGCTTG TGAGAACTGT GCCAGCCTGA TGAAAAAGTG TGTGCAGTGT 
CGGGCAGTGG TTGAGCGGAG GGTGCCTTTC ATCACGTGCT GTGGTGGCAA GAGCTCAGAA 
GACCCCTCTG ATGAAATCTC AAGTGGGAAT ATCCCAGTGC TGCAGAAGGA CAAAGACAAC 
ACCAATGTTA ACGCAGATGT GCAGAAACTG CAGCAGCAGC TTCAGGACAT TAAGGAGCAG 
ACCATGTGCC CTGTGTGCTT GGATCGCCTG AAGAACATGA TTTTCCTCTG TGGCCATGGA 
ACATGCCAGC TCTGTGGAGA CCGGATGAGT GAATGCCCAA TTTGTCGCAA GGCAATTGAA 
CGAAGGATTC TTTTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525873.1
CDS	753..3773
Translation	MSNSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFITCCGGKSSEDPSDEISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_006525810.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 1 (Mib1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525810.2

ORF Insert Sequence:

ATGAGTAACT CCCGCAACAA CCGAGTGATG GTGGAGGGGG TCGGCGCCCG CGTAGTGCGC 
GGCCCGGACT GGAAGTGGGG CAAGCAGGAC GGCGGCGAGG GCCATGTAGG CACTGTACGG 
AGCTTCGAGA GTCCGGAGGA GGTCGTGGTG GTGTGGGACA ACGGCACGGC CGCCAACTAC 
CGCTGCTCAG GGGCCTACGA CCTGCGTATC CTGGACAGCG CGCCCACCGG CATCAAGCAT 
GATGGAACTA TGTGTGATAC CTGCCGCCAG CAACCAATCA TTGGCATTCG ATGGAAATGT 
GCAGAGTGCA CCAATTATGA TTTGTGCACT GTTTGTTATC ATGGAGATAA ACATCACTTA 
AGGCATCGCT TTTATAGGAT TACTACACCA GGAAGTGAAC GGGTTCTGTT AGAGTCTCGT 
AGAAAATCTA AGAAGATTAC AGCCAGAGGA ATCTTTGCTG GTGCCAGAGT GGTGCGAGGA 
GTGGACTGGC AGTGGGAAGA TCAAGATGGA GGCAATGGGC GTAGAGGAAA GGTCACGGAG 
ATCCAGGACT GGAGTGCATC CAGCCCACAC AGTGCAGCCT ATGTTCTCTG GGATAACGGT 
GCTAAGAACC TTTACAGGGT TGGCTTTGAG GGCATGTCTG ATCTCAAGTG TGTCCAGGAT 
GCCAAAGGAG GTTCTTTCTA CAGAGATCAT TGCCCTGTGC TAGGTGAGCA GAATGGCAAC 
AGGAATCCTG GTGGATTGCA GATTGGTGAC CTGGTAAATA TAGACCTTGA TCTAGAAATT 
GTCCAGTCTT TACAGCACGG TCATGGAGGA TGGACCGATG GCATGTTTGA GACTTTAACT 
ACAACTGGAA CCGTCTGTGG CATTGATGAA GATCATGACA TTGTAGTACA GTATCCAAGT 
GGCAATAGAT GGACTTTCAA TCCTGCTGTT CTCACTAAGG CAAACATTGT CCGAAGTGGG 
GATGCTGCAC AGGGTGCAGA AGGAGGCACC TCACAGTTTC AAGTGGGTGA TCTTGTGCAA 
GTTTGTTATG ATCTGGAAAG AATTAAACTT CTCCAAAGAG GACATGGTGA ATGGGCTGAA 
GCAATGCTTC CAACTTTAGG TAAAGTTGGC CGAGTACAAC AGATTTATTC GGACAGTGAC 
TTAAAGGTGG AAGTCTGTGG AACATCTTGG ACGTATAACC CTGCAGCAGT TTCCAAGGTG 
GCCCCTGCAG GATCAGCCAT TAGCAATGCA TCTGGTGAAA GACTCTCCCA GCTTCTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGTGAC CTCAATGAAG AGCTGGTTAA GGCTGCTGCC 
AATGGAGACG TGGCTAAAGT GGAAGATTTG CTGAAAAGAC CAGATGTGGA TGTAAACGGA 
CAGTGTGCTG GCCACACAGC AATGCAAGCT GCTAGTCAGA ACGGACATGT TGATATTTTG 
AAATTACTTT TGAAGCAAAA TGTTGATGTT GAAGCAGAGG ATAAAGATGG AGATCGAGCC 
GTTCACCATG CAGCCTTTGG AGATGAGGGT GCTGTCATAG AAGTCCTACA CCGAGGCAGT 
GCTGACCTGA ATGCTCGTAA TAAGCGTAGA CAGACGCCTC TTCATATTGC TGTCAATAAA 
GGTCATCTTC AAGTTGTGAA GACTTTGTTG GATTTTGGTT GTCATCCCAG TCTCCAGGAT 
TCTGAAGGTG ACACCCCTCT CCATGATGCC ATAAGTAAGA AACGGGATGA CATTCTGGCA 
GTTCTTCTGG AAGCTGGAGC AGATGTGACC ATCACGAACA ATAACGGGTT CAATGCCCTG 
CACCATGCTG CACTGAGAGG GAACCCCAGT GCAATGCGTG TTTTACTCTC AAAATTACCA 
AGACCATGGA TTGTGGATGA GAAGAAAGAT GATGGTTATA CTGCCTTGCA CCTGGCTGCC 
CTGAATAATC ATGTGGAAGT GGCTGAGCTG TTGGTGCATC AGGGAAATGC AAACCTGGAT 
ATCCAGAATG TGAACCAGCA AACAGCCCTA CACCTTGCTG TTGAACGGCA ACACACCCAG 
ATTGTTAGGC TTTTGGTCCG TGCAGGTGCT AAGCTAGACA TTCAGGATAA GGATGGAGAT 
ACTCCTCTGC ATGAAGCTTT GAGACATCAT ACTTTGTCTC AACTACGTCA GCTACAAGAC 
ATGCAAGATG TGGGCAAGGT AGACGCTGCC TGGGAACCTT CAAAAAACAC ATTAATAATG 
GGACTTGGTA CCCAGGGGGC TGAGAAGAAG AGTGCAGCAT CCATTGCGTG TTTCTTGGCA 
GCCAATGGTG CTGATCTCAG CATTCGAAAT AAGAAGGGTC AGTCACCACT GGATCTCTGT 
CCTGACCCAA GTCTCTGCAA AGCTTTGGCA AAATGTCATA AGGAAAAAGT CAGTGGCCAA 
GTGGGTTCTC GGAGTCCTTC TATGATTAGC AACGATTCTG AAACTTTAGA GGAATGTATG 
GTGTGCTCAG ACATGAAGAG AGATACTCTT TTTGGTCCGT GTGGACACAT TGCAACCTGT 
TCTTTGTGTT CCCCTCGTGT CAAGAAATGC CTCATTTGTA AAGAGCAGGT TCAGTCCAGG 
ACAAAGATTG AAGAATGTGT GGTGTGCTCC GACAAGAAAG CAGCTGTTCT CTTTCAACCT 
TGTGGGCACA TGTGTGCTTG TGAGAACTGT GCCAGCCTGA TGAAAAAGTG TGTGCAGTGT 
CGGGCAGTGG TTGAGCGGAG GGTGCCTTTC ATCACGTGCT GTGGTGGCAA GAGCTCAGAA 
GACCCCTCTG ATGAAATCTC AAGTGGGAAT ATCCCAGTGC TGCAGAAGGA CAAAGACAAC 
ACCAATGTTA ACGCAGATGT GCAGAAACTG CAGCAGCAGC TTCAGGACAT TAAGGAGCAG 
ACCATGTGCC CTGTGTGCTT GGATCGCCTG AAGAACATGA TTTTCCTCTG TGGCCATGGA 
ACATGCCAGC TCTGTGGAGA CCGGATGAGT GAATGCCCAA TTTGTCGCAA GGCAATTGAA 
CGAAGGATTC TTTTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of Usp9x disrupts cell adhesion, and components of the Wnt and Notch signaling pathways in neural progenitors.
Scientific reports7(1)8109(2017 Aug)
Premarathne S,Murtaza M,Matigian N,Jolly LA,Wood SA

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Mib1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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