Safb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Safb
Entrez Gene ID	224903
Full Name	scaffold attachment factor B
Synonyms	3110021E02Rik,5330423C17Rik,AU018122,D18386,E130307D12,HAP,HET,SAF-B1,SAFB1
General protein information	Preferred Names scaffold attachment factor B Names scaffold attachment factor B1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 D

mRNA and Protein(s)

mRNA	Protein	Name
XM_006524160.4	XP_006524223.1	scaffold attachment factor B1 isoform X1
XM_017317445.1	XP_017172934.1	scaffold attachment factor B1 isoform X2
XM_006524160.3	XP_006524223.1	scaffold attachment factor B1 isoform X1
NM_001163300.1	NP_001156772.1	scaffold attachment factor B1 isoform 2
NM_001374619.1	NP_001361548.1	scaffold attachment factor B1 isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Safb	NP_071789.1
Pan troglodytes (chimpanzee)	SAFB	XP_001142439.3
Gallus gallus (chicken)	SAFB	XP_423726.3
Homo sapiens (human)	SAFB	NP_001188267.1
Mus musculus (house mouse)	Safb	NP_001156772.1
Gallus gallus (chicken)	SAFB2	XP_004948787.1
	SAFB	XP_001085118.2
Canis lupus familiaris (dog)	SAFB	XP_005633287.1
Danio rerio (zebrafish)	safb	NP_999848.1
Bos taurus (cattle)	SAFB	NP_001093185.1

Gene Ontology
Bibliography

Related articles in PubMed

Scaffold attachment factor B2 (SAFB2)-null mice reveal non-redundant functions of SAFB2 compared with its paralog, SAFB1.
Jiang S, Katz TA, Garee JP, DeMayo FJ, Lee AV, Oesterreich S
Disease models & mechanisms8(9)1121-7(2015 Sep)

The Werner syndrome helicase protein is required for cell proliferation, immortalization, and tumorigenesis in Scaffold attachment factor B1 deficient mice.
Lachapelle S, Oesterreich S, Lebel M
Aging3(3)277-90(2011 Mar)

SAFB1, an RBMX-binding protein, is a newly identified regulator of hepatic SREBP-1c gene.
Omura Y, Nishio Y, Takemoto T, Ikeuchi C, Sekine O, Morino K, Maeno Y, Obata T, Ugi S, Maegawa H, Kimura H, Kashiwagi A
BMB reports42(4)232-7(2009 Apr)

Scaffold Attachment Factor B1 (SAFB1) heterozygosity does not influence Wnt-1 or DMBA-induced tumorigenesis.
Kaipparettu BA, Dobrzycka KM, Britton O, Lee AV, Herron AJ, Li Y, Lewis MT, Medina D, Oesterreich S
Molecular cancer815(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

despite a high degree of sequence similarity, SAFB1(-/-) and SAFB2(-/-) mice do not totally phenocopy each other
Title: Scaffold attachment factor B2 (SAFB2)-null mice reveal non-redundant functions of SAFB2 compared with its paralog, SAFB1.

Mice lacking SAFB1 exhibit developmental abnormalities in their lungs, high incidence of perinatal lethality, and adults develop different types of tumors. Mouse embryonic fibroblasts from Safb1-null animals are immortalized in culture.
Title: The Werner syndrome helicase protein is required for cell proliferation, immortalization, and tumorigenesis in Scaffold attachment factor B1 deficient mice.

These results indicate a major role for SAFB1 in the activation of Srebp-1c through its interaction with RBMX.
Title: SAFB1, an RBMX-binding protein, is a newly identified regulator of hepatic SREBP-1c gene.

Our data show that SAFB1 heterozygosity does not influence Wnt-1 induced tumorigenesis
Title: Scaffold Attachment Factor B1 (SAFB1) heterozygosity does not influence Wnt-1 or DMBA-induced tumorigenesis.

SAFB1 loss causes lack of contact inhibition, increased foci formation, and increased oncogene-induced anchorage-independent growth.
Title: Disruption of scaffold attachment factor B1 leads to TBX2 up-regulation, lack of p19ARF induction, lack of senescence, and cell immortalization.

The following Safb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Safb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08872	NM_001163300.1 Latest version!	Mus musculus scaffold attachment factor B (Safb), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$552.30 $789.00
OMu53508	NM_001374619.1 Latest version!	Mus musculus scaffold attachment factor B (Safb), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu53508	XM_006524160.4 Latest version!	Mus musculus scaffold attachment factor B (Safb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu79576	XM_017317445.1 Latest version!	Mus musculus scaffold attachment factor B (Safb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu53508	XM_006524160.3	Mus musculus scaffold attachment factor B (Safb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Safb ( NM_001163300.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156772.1 Mus musculus scaffold attachment factor B (Safb), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu08872
Clone ID Related Accession (Same CDS sequence)	NM_001163300.1
Accession Version	NM_001163300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-06
Organism	Mus musculus(house mouse)
Product	scaffold attachment factor B1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT485788.18. On or before Jul 17, 2009 this sequence version replaced XM_924956.2, XM_001480312.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGA CTCTGTCGGG TTTAGGAGAC GCGAGTGCGG CGGGCGCGGC GGCCGTGAGC 
TCCGCGGCGT CAGAGACCGG GACGCGGCGG CTCAGCGACC TGCGGGTGAT CGACCTGCGG 
GCGGAGCTGA AGAAGCGGAA CCTCGACTCG AGCGGCAACA AGAGCGTGCT GATGGAGCGG 
CTGAAGAAGG CCATTGAAGA TGAAGGTGGG AATCCTGATG AAATTGAAGT CACCTCAGAA 
TGCAACAAGA AGATGCCTAA GAGGCCCAGC AAAGGGAGGA AACCAGAAGA CGAGGGTGTG 
GAGGACAACG GGCTCGAGGA GAATTCTGGG GATGGGCAGG AGGATGTGGA GACCAGCCTG 
GAGAACCTGC AAGACATGGA TATGATGGAC ATCAGTGTGC TGGATGAGGC TGACATTGAC 
AATGGCAGCG TGGCAGACTG TGTGGAGGAA GAGGAGGAGG CCACACTCCC TGAGGGCCTA 
GCAGACAGCA CTGAACTGGT AGAAGGAGAT TTGAAAGGGC TCCCAGAACA GCTGCAGGAG 
CATGCTATAG ATGACAAGGA CACCGTTAAC AATGTAGATA CGTCATCATC TGACTTCACC 
ATGCTGCAGG AGATGGAAGA GGCATCTTTG GAGCCAGAAA ATGAGAAAAT ACTCGACATT 
TTGGGGGAAA CTTGTAAATC TGAGCCAGTA AAAGAAGAAG GTTCGGAGCT GGAGCAGCCC 
TTTGCCCAGG CCACGAGTAG CGTGGGGCCA GACAGGAAGC TGGCGGAGGA AGAGGACCTA 
TTTGAGAGCT GCGGCCACCC GGAAGAGGAA GAGGAAGAGG AGGAAGAAGA TCAGGAAGAG 
GAGCAGGAGG AGGAGGGAGA TTTAGCTTTG GCCAGCAGCA GCAAGTCTGA GTCTCCAAGC 
ACTCGGTGTC AGTGGAGCGA GGCAGATGCC CCGTTAGCAG TAGTGAAAAG GGAGCCGGCA 
GATGCGCCTG GCGGAGGCAC TGGGATGGAC CGCGAGCCTG TAGGGCTAGA GGAGCCAGTT 
GAACAGAGTA GCACGGCTGC CCAGCTCCCG GAGGCCACCA GCCAGGAGCT GGTGCGAGCG 
CCCACGGCAG CCCTGAGCCC TGAGCCCCAA GATAGCAAAG AAGACGTGAA GAAGTTTGCT 
TTTGACGCTT GTAATGACGT CCCTGCGCCT CCTAAAGAGT CCTCAGCCAG TGAGGGCGCT 
GATCAGAAAA TGAGTTCTGT GGAGGAGGAC TCGGATACAA AAAGGCTTTC CCGAGAAGAG 
AAGGGTCGCA GCAGCTGTGG TAGGAATTTC TGGGTTAGTG GCCTTTCGTC TACAACCAGA 
GCCACGGATC TGAAGAACCT GTTTAGCAGA TATGGGAAGG TGGTGGGTGC TAAGGTTGTG 
ACGAACGCCC GGAGTCCTGG AGCTCGCTGT TATGGCTTTG TCACTATGTC CACAGCTGAA 
GAGGCAACAA AATGCATTAG CCATTTGCAC AAGACTGAGC TGCATGGCAA GATGATCTCA 
GTGGAAAAGG CCAAAAGTGA GCCCACTGGC AAAAGAGTGC CTGACAGGCG AGATGGGGAC 
AGCAAGAAGG AGAAGGCCAG CACCAGTGAC AGATCTGCAA ACCTTAAGAG GGAGGAGAAA 
GGTGAGAGAA AAGACGATGC CAAGAAGACA GATGATGGGA GCACAGAGAA GAGCAAAGAC 
GCAGATGACC AGAAACCTGG GCCTTCAGAG CGCTCGCGAA CGACCAAGTC AGGAAGCCGT 
GGCACTGAGC GCACTGTGGT GATGGACAAG TCCAAAGGTG TGCCTGTCAT CAGTGTGAAA 
ACATCTGGGT CTAAAGAGAG AGCCTCCAAA AGTCAGGACC GGAAGTCAGC CAGCCGGGAG 
AAGCGGTCTG TTGTGTCCTT TGACAAAGTT AAAGAGTCTC GAAAGTCCAG AGACTCAGAG 
TCTCGGAGGG AACGGGAGCG CAGTGAGCGG GAGCAGAGGC TGCAGGCCCA GTGGGAGCGG 
GAGGAGCGAG AGCGGCTGGA GATTGCTCGT GAGCGCTTGG CATTCCACCG CCATCGGCTA 
GAGCGTGAAC GCATGGAGCG GGAGCGGCTG GAGCGGGAGC GGATGCATGT AGAACAGGAG 
CGGAGGCGTG AGCAAGAGCG CATTCACCGA GAGCGAGAGG AGCTTCGGCG TCAGCAGGAG 
CTGCGCTATG AACAGGAGCG CCGGCCTGCT GTGCGCAGGC CCTATGAAGT GGATGGCCGG 
CGAGATGATG CCTATTGGCC AGAAGCTAAA CGGGCTGCTC TGGATGACCG TTACCACTCA 
GACTTCAGCC GCCAGGACCG CTTTCACGAC TTCGACCACA GAGACAGAGG CCGTTATCCC 
AACCATTCTG TGGATAGGAG AGAAGGTTCA CGGTCGATGA TGGGAGACAG AGAAGGACAG 
CATTACCCTG AACGCCATGG AGGACCTGAA CGCCATGGCC GGGACTCTCG AGATGGCTGG 
GGCTATGGTT CGAACAAGAG ATTGAGTGAA GGCCGGGGGC TGCCCCCTCC TCCCAGGCGT 
GACTGGGGGG AGCATGGCCG GCGACTTGAA GATGACCGGG CATGGCAGGG CACGGCTGAT 
GGAGGCATGA TGGAGCGTGA TCATAAGAGG TGGCAAGGTG GTGAGAGGAG CATGTCTGGC 
CACTCGGGGC CTGGTCACAT GATGAACCGT GGTGGCATGT CAGGGCGAGG CAGTTTTGCC 
CCAGGAGGAG CTTCTCGTGG CCATGTGATC CCACGTGGAG GGATGCAGGC AGGCTTTGGA 
GGACAGAGCC GGGGAAGCAG ACCCAGTGAT GCCCGCTTCA CCCGCCGCTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156772.1
CDS	181..2994
Translation	MAETLSGLGDASAAGAAAVSSAASETGTRRLSDLRVIDLRAELKKRNLDSSGNKSVLMERLKKAIEDEGGNPDEIEVTSECNKKMPKRPSKGRKPEDEGVEDNGLEENSGDGQEDVETSLENLQDMDMMDISVLDEADIDNGSVADCVEEEEEATLPEGLADSTELVEGDLKGLPEQLQEHAIDDKDTVNNVDTSSSDFTMLQEMEEASLEPENEKILDILGETCKSEPVKEEGSELEQPFAQATSSVGPDRKLAEEEDLFESCGHPEEEEEEEEEDQEEEQEEEGDLALASSSKSESPSTRCQWSEADAPLAVVKREPADAPGGGTGMDREPVGLEEPVEQSSTAAQLPEATSQELVRAPTAALSPEPQDSKEDVKKFAFDACNDVPAPPKESSASEGADQKMSSVEEDSDTKRLSREEKGRSSCGRNFWVSGLSSTTRATDLKNLFSRYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCISHLHKTELHGKMISVEKAKSEPTGKRVPDRRDGDSKKEKASTSDRSANLKREEKGERKDDAKKTDDGSTEKSKDADDQKPGPSERSRTTKSGSRGTERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSASREKRSVVSFDKVKESRKSRDSESRRERERSEREQRLQAQWEREERERLEIARERLAFHRHRLERERMERERLERERMHVEQERRREQERIHREREELRRQQELRYEQERRPAVRRPYEVDGRRDDAYWPEAKRAALDDRYHSDFSRQDRFHDFDHRDRGRYPNHSVDRREGSRSMMGDREGQHYPERHGGPERHGRDSRDGWGYGSNKRLSEGRGLPPPPRRDWGEHGRRLEDDRAWQGTADGGMMERDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHVIPRGGMQAGFGGQSRGSRPSDARFTRRY

Target ORF information:

RefSeq Version	NM_001163300.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus scaffold attachment factor B (Safb), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163300.1

ORF Insert Sequence:

ATGGCGGAGA CTCTGTCGGG TTTAGGAGAC GCGAGTGCGG CGGGCGCGGC GGCCGTGAGC 
TCCGCGGCGT CAGAGACCGG GACGCGGCGG CTCAGCGACC TGCGGGTGAT CGACCTGCGG 
GCGGAGCTGA AGAAGCGGAA CCTCGACTCG AGCGGCAACA AGAGCGTGCT GATGGAGCGG 
CTGAAGAAGG CCATTGAAGA TGAAGGTGGG AATCCTGATG AAATTGAAGT CACCTCAGAA 
TGCAACAAGA AGATGCCTAA GAGGCCCAGC AAAGGGAGGA AACCAGAAGA CGAGGGTGTG 
GAGGACAACG GGCTCGAGGA GAATTCTGGG GATGGGCAGG AGGATGTGGA GACCAGCCTG 
GAGAACCTGC AAGACATGGA TATGATGGAC ATCAGTGTGC TGGATGAGGC TGACATTGAC 
AATGGCAGCG TGGCAGACTG TGTGGAGGAA GAGGAGGAGG CCACACTCCC TGAGGGCCTA 
GCAGACAGCA CTGAACTGGT AGAAGGAGAT TTGAAAGGGC TCCCAGAACA GCTGCAGGAG 
CATGCTATAG ATGACAAGGA CACCGTTAAC AATGTAGATA CGTCATCATC TGACTTCACC 
ATGCTGCAGG AGATGGAAGA GGCATCTTTG GAGCCAGAAA ATGAGAAAAT ACTCGACATT 
TTGGGGGAAA CTTGTAAATC TGAGCCAGTA AAAGAAGAAG GTTCGGAGCT GGAGCAGCCC 
TTTGCCCAGG CCACGAGTAG CGTGGGGCCA GACAGGAAGC TGGCGGAGGA AGAGGACCTA 
TTTGAGAGCT GCGGCCACCC GGAAGAGGAA GAGGAAGAGG AGGAAGAAGA TCAGGAAGAG 
GAGCAGGAGG AGGAGGGAGA TTTAGCTTTG GCCAGCAGCA GCAAGTCTGA GTCTCCAAGC 
ACTCGGTGTC AGTGGAGCGA GGCAGATGCC CCGTTAGCAG TAGTGAAAAG GGAGCCGGCA 
GATGCGCCTG GCGGAGGCAC TGGGATGGAC CGCGAGCCTG TAGGGCTAGA GGAGCCAGTT 
GAACAGAGTA GCACGGCTGC CCAGCTCCCG GAGGCCACCA GCCAGGAGCT GGTGCGAGCG 
CCCACGGCAG CCCTGAGCCC TGAGCCCCAA GATAGCAAAG AAGACGTGAA GAAGTTTGCT 
TTTGACGCTT GTAATGACGT CCCTGCGCCT CCTAAAGAGT CCTCAGCCAG TGAGGGCGCT 
GATCAGAAAA TGAGTTCTGT GGAGGAGGAC TCGGATACAA AAAGGCTTTC CCGAGAAGAG 
AAGGGTCGCA GCAGCTGTGG TAGGAATTTC TGGGTTAGTG GCCTTTCGTC TACAACCAGA 
GCCACGGATC TGAAGAACCT GTTTAGCAGA TATGGGAAGG TGGTGGGTGC TAAGGTTGTG 
ACGAACGCCC GGAGTCCTGG AGCTCGCTGT TATGGCTTTG TCACTATGTC CACAGCTGAA 
GAGGCAACAA AATGCATTAG CCATTTGCAC AAGACTGAGC TGCATGGCAA GATGATCTCA 
GTGGAAAAGG CCAAAAGTGA GCCCACTGGC AAAAGAGTGC CTGACAGGCG AGATGGGGAC 
AGCAAGAAGG AGAAGGCCAG CACCAGTGAC AGATCTGCAA ACCTTAAGAG GGAGGAGAAA 
GGTGAGAGAA AAGACGATGC CAAGAAGACA GATGATGGGA GCACAGAGAA GAGCAAAGAC 
GCAGATGACC AGAAACCTGG GCCTTCAGAG CGCTCGCGAA CGACCAAGTC AGGAAGCCGT 
GGCACTGAGC GCACTGTGGT GATGGACAAG TCCAAAGGTG TGCCTGTCAT CAGTGTGAAA 
ACATCTGGGT CTAAAGAGAG AGCCTCCAAA AGTCAGGACC GGAAGTCAGC CAGCCGGGAG 
AAGCGGTCTG TTGTGTCCTT TGACAAAGTT AAAGAGTCTC GAAAGTCCAG AGACTCAGAG 
TCTCGGAGGG AACGGGAGCG CAGTGAGCGG GAGCAGAGGC TGCAGGCCCA GTGGGAGCGG 
GAGGAGCGAG AGCGGCTGGA GATTGCTCGT GAGCGCTTGG CATTCCACCG CCATCGGCTA 
GAGCGTGAAC GCATGGAGCG GGAGCGGCTG GAGCGGGAGC GGATGCATGT AGAACAGGAG 
CGGAGGCGTG AGCAAGAGCG CATTCACCGA GAGCGAGAGG AGCTTCGGCG TCAGCAGGAG 
CTGCGCTATG AACAGGAGCG CCGGCCTGCT GTGCGCAGGC CCTATGAAGT GGATGGCCGG 
CGAGATGATG CCTATTGGCC AGAAGCTAAA CGGGCTGCTC TGGATGACCG TTACCACTCA 
GACTTCAGCC GCCAGGACCG CTTTCACGAC TTCGACCACA GAGACAGAGG CCGTTATCCC 
AACCATTCTG TGGATAGGAG AGAAGGTTCA CGGTCGATGA TGGGAGACAG AGAAGGACAG 
CATTACCCTG AACGCCATGG AGGACCTGAA CGCCATGGCC GGGACTCTCG AGATGGCTGG 
GGCTATGGTT CGAACAAGAG ATTGAGTGAA GGCCGGGGGC TGCCCCCTCC TCCCAGGCGT 
GACTGGGGGG AGCATGGCCG GCGACTTGAA GATGACCGGG CATGGCAGGG CACGGCTGAT 
GGAGGCATGA TGGAGCGTGA TCATAAGAGG TGGCAAGGTG GTGAGAGGAG CATGTCTGGC 
CACTCGGGGC CTGGTCACAT GATGAACCGT GGTGGCATGT CAGGGCGAGG CAGTTTTGCC 
CCAGGAGGAG CTTCTCGTGG CCATGTGATC CCACGTGGAG GGATGCAGGC AGGCTTTGGA 
GGACAGAGCC GGGGAAGCAG ACCCAGTGAT GCCCGCTTCA CCCGCCGCTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Safb ( XM_006524160.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524223.1 Mus musculus scaffold attachment factor B (Safb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53508
Clone ID Related Accession (Same CDS sequence)	XM_006524160.4 , XM_006524160.3 , NM_001374619.1
Accession Version	XM_006524160.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	scaffold attachment factor B1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524160.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGA CTCTGTCGGG TTTAGGAGAC GCGAGTGCGG CGGGCGCGGC GGCCGTGAGC 
TCCGCGGCGT CAGAGACCGG GACGCGGCGG CTCAGCGACC TGCGGGTGAT CGACCTGCGG 
GCGGAGCTGA AGAAGCGGAA CCTCGACTCG AGCGGCAACA AGAGCGTGCT GATGGAGCGG 
CTGAAGAAGG CCATTGAAGA TGAAGGTGGG AATCCTGATG AAATTGAAGT CACCTCAGAA 
TGCAACAAGA AGATGCCTAA GAGGCCCAGC AAAGGGAGGA AACCAGAAGA CGAGGGTGTG 
GAGGACAACG GGCTCGAGGA GAATTCTGGG GATGGGCAGG AGGATGTGGA GACCAGCCTG 
GAGAACCTGC AAGACATGGA TATGATGGAC ATCAGTGTGC TGGATGAGGC TGACATTGAC 
AATGGCAGCG TGGCAGACTG TGTGGAGGAA GAGGAGGAGG CCACACTCCC TGAGGGCCTA 
GCAGACAGCA CTGAACTGGT AGAAGGAGAT TTGAAAGGGC TCCCAGAACA GCTGCAGGAG 
CATGCTATAG ATGACAAGGA CACCGTTAAC AATGTAGATA CGTCATCATC TGACTTCACC 
ATGCTGCAGG AGATGGAAGA GGCATCTTTG GAGCCAGAAA ATGAGAAAAT ACTCGACATT 
TTGGGGGAAA CTTGTAAATC TGAGCCAGTA AAAGAAGAAG GTTCGGAGCT GGAGCAGCCC 
TTTGCCCAGG CCACGAGTAG CGTGGGGCCA GACAGGAAGC TGGCGGAGGA AGAGGACCTA 
TTTGAGAGCT GCGGCCACCC GGAAGAGGAA GAGGAAGAGG AGGAAGAAGA TCAGGAAGAG 
GAGCAGGAGG AGGAGGGAGA TTTAGCTTTG GCCAGCAGCA GCAAGTCTGA GTCTCCAAGC 
ACTCGGTGTC AGTGGAGCGA GGCAGATGCC CCGTTAGCAG TAGTGAAAAG GGAGCCGGCA 
GATGCGCCTG GCGGAGGCAC TGGGATGGAC CGCGAGCCTG TAGGGCTAGA GGAGCCAGTT 
GAACAGAGTA GCACGGCTGC CCAGCTCCCG GAGGCCACCA GCCAGGAGCT GGTGCGAGCG 
CCCACGGCAG CCCTGAGCCC TGAGCCCCAA GATAGCAAAG AAGACGTGAA GAAGTTTGCT 
TTTGACGCTT GTAATGACGT CCCTGCGCCT CCTAAAGAGT CCTCAGCCAG TGAGGGCGCT 
GATCAGAAAA TGAGTTCTGT GGAGGAGGAC TCGGATACAA AAAGGCTTTC CCGAGAAGAG 
AAGGGTCGCA GCAGCTGTGG TAGGAATTTC TGGGTTAGTG GCCTTTCGTC TACAACCAGA 
GCCACGGATC TGAAGAACCT GTTTAGCAGA TATGGGAAGG TGGTGGGTGC TAAGGTTGTG 
ACGAACGCCC GGAGTCCTGG AGCTCGCTGT TATGGCTTTG TCACTATGTC CACAGCTGAA 
GAGGCAACAA AATGCATTAG CCATTTGCAC AAGACTGAGC TGCATGGCAA GATGATCTCA 
GTGGAAAAGG CCAAAAGTGA GCCCACTGGC AAAAGAGTGC CTGACAGGCG AGATGGGGAC 
AGCAAGAAGG AGAAGGCCAG CACCAGTGAC AGATCTGCAA ACCTTAAGAG GGAGGAGAAA 
GGTGAGAGAA AAGACGATGC CAAGAAGACA GATGATGGGA GCACAGAGAA GAGCAAAGAC 
GCAGATGACC AGAAACCTGG GCCTTCAGAG CGCTCGCGAA CGACCAAGTC AGGAAGCCGT 
GGCACTGAGC GCACTGTGGT GATGGACAAG TCCAAAGGTG TGCCTGTCAT CAGTGTGAAA 
ACATCTGGGT CTAAAGAGAG AGCCTCCAAA AGTCAGGACC GGAAGTCAGC CAGCCGGGAG 
AAGCGGTCTG TTGTGTCCTT TGACAAAGTT AAAGAGTCTC GAAAGTCCAG AGACTCAGAG 
TCTCGGAGGG AACGGGAGCG CAGTGAGCGG GAGCAGAGGC TGCAGGCCCA GTGGGAGCGG 
GAGGAGCGAG AGCGGCTGGA GATTGCTCGT GAGCGCTTGG CATTCCACCG CCATCGGCTA 
GAGCGTGAAC GCATGGAGCG GGAGCGGCTG GAGCGGGAGC GGATGCATGT AGAACAGGAG 
CGGAGGCGTG AGCAAGAGCG CATTCACCGA GAGCGAGAGG AGCTTCGGCG TCAGCAGGAG 
CTGCGCTATG AACAGGAGCG CCGGCCTGCT GTGCGCAGGC CCTATGAAGT GGATGGCCGG 
CGAGATGATG CCTATTGGCC AGAAGCTAAA CGGGCTGCTC TGGATGACCG TTACCACTCA 
GACTTCAGCC GCCAGGACCG CTTTCACGAC TTCGACCACA GAGACAGAGG CCGTTATCCC 
AACCATTCTG TGGATAGGAG AGAAGGTTCA CGGTCGATGA TGGGAGACAG AGAAGGACAG 
CATTACCCTG AACGCCATGG AGGACCTGAA CGCCATGGCC GGGACTCTCG AGATGGCTGG 
GGCTATGGTT CGAACAAGAG ATTGAGTGAA GGCCGGGGGC TGCCCCCTCC TCCCAGGGGC 
AGGCGTGACT GGGGGGAGCA TGGCCGGCGA CTTGAAGATG ACCGGGCATG GCAGGGCACG 
GCTGATGGAG GCATGATGGA GCGTGATCAT AAGAGGTGGC AAGGTGGTGA GAGGAGCATG 
TCTGGCCACT CGGGGCCTGG TCACATGATG AACCGTGGTG GCATGTCAGG GCGAGGCAGT 
TTTGCCCCAG GAGGAGCTTC TCGTGGCCAT GTGATCCCAC GTGGAGGGAT GCAGGCAGGC 
TTTGGAGGAC AGAGCCGGGG AAGCAGACCC AGTGATGCCC GCTTCACCCG CCGCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524223.1
CDS	333..3152
Translation	MAETLSGLGDASAAGAAAVSSAASETGTRRLSDLRVIDLRAELKKRNLDSSGNKSVLMERLKKAIEDEGGNPDEIEVTSECNKKMPKRPSKGRKPEDEGVEDNGLEENSGDGQEDVETSLENLQDMDMMDISVLDEADIDNGSVADCVEEEEEATLPEGLADSTELVEGDLKGLPEQLQEHAIDDKDTVNNVDTSSSDFTMLQEMEEASLEPENEKILDILGETCKSEPVKEEGSELEQPFAQATSSVGPDRKLAEEEDLFESCGHPEEEEEEEEEDQEEEQEEEGDLALASSSKSESPSTRCQWSEADAPLAVVKREPADAPGGGTGMDREPVGLEEPVEQSSTAAQLPEATSQELVRAPTAALSPEPQDSKEDVKKFAFDACNDVPAPPKESSASEGADQKMSSVEEDSDTKRLSREEKGRSSCGRNFWVSGLSSTTRATDLKNLFSRYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCISHLHKTELHGKMISVEKAKSEPTGKRVPDRRDGDSKKEKASTSDRSANLKREEKGERKDDAKKTDDGSTEKSKDADDQKPGPSERSRTTKSGSRGTERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSASREKRSVVSFDKVKESRKSRDSESRRERERSEREQRLQAQWEREERERLEIARERLAFHRHRLERERMERERLERERMHVEQERRREQERIHREREELRRQQELRYEQERRPAVRRPYEVDGRRDDAYWPEAKRAALDDRYHSDFSRQDRFHDFDHRDRGRYPNHSVDRREGSRSMMGDREGQHYPERHGGPERHGRDSRDGWGYGSNKRLSEGRGLPPPPRGRRDWGEHGRRLEDDRAWQGTADGGMMERDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHVIPRGGMQAGFGGQSRGSRPSDARFTRRY

Target ORF information:

RefSeq Version	XM_006524160.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus scaffold attachment factor B (Safb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524160.3

ORF Insert Sequence:

ATGGCGGAGA CTCTGTCGGG TTTAGGAGAC GCGAGTGCGG CGGGCGCGGC GGCCGTGAGC 
TCCGCGGCGT CAGAGACCGG GACGCGGCGG CTCAGCGACC TGCGGGTGAT CGACCTGCGG 
GCGGAGCTGA AGAAGCGGAA CCTCGACTCG AGCGGCAACA AGAGCGTGCT GATGGAGCGG 
CTGAAGAAGG CCATTGAAGA TGAAGGTGGG AATCCTGATG AAATTGAAGT CACCTCAGAA 
TGCAACAAGA AGATGCCTAA GAGGCCCAGC AAAGGGAGGA AACCAGAAGA CGAGGGTGTG 
GAGGACAACG GGCTCGAGGA GAATTCTGGG GATGGGCAGG AGGATGTGGA GACCAGCCTG 
GAGAACCTGC AAGACATGGA TATGATGGAC ATCAGTGTGC TGGATGAGGC TGACATTGAC 
AATGGCAGCG TGGCAGACTG TGTGGAGGAA GAGGAGGAGG CCACACTCCC TGAGGGCCTA 
GCAGACAGCA CTGAACTGGT AGAAGGAGAT TTGAAAGGGC TCCCAGAACA GCTGCAGGAG 
CATGCTATAG ATGACAAGGA CACCGTTAAC AATGTAGATA CGTCATCATC TGACTTCACC 
ATGCTGCAGG AGATGGAAGA GGCATCTTTG GAGCCAGAAA ATGAGAAAAT ACTCGACATT 
TTGGGGGAAA CTTGTAAATC TGAGCCAGTA AAAGAAGAAG GTTCGGAGCT GGAGCAGCCC 
TTTGCCCAGG CCACGAGTAG CGTGGGGCCA GACAGGAAGC TGGCGGAGGA AGAGGACCTA 
TTTGAGAGCT GCGGCCACCC GGAAGAGGAA GAGGAAGAGG AGGAAGAAGA TCAGGAAGAG 
GAGCAGGAGG AGGAGGGAGA TTTAGCTTTG GCCAGCAGCA GCAAGTCTGA GTCTCCAAGC 
ACTCGGTGTC AGTGGAGCGA GGCAGATGCC CCGTTAGCAG TAGTGAAAAG GGAGCCGGCA 
GATGCGCCTG GCGGAGGCAC TGGGATGGAC CGCGAGCCTG TAGGGCTAGA GGAGCCAGTT 
GAACAGAGTA GCACGGCTGC CCAGCTCCCG GAGGCCACCA GCCAGGAGCT GGTGCGAGCG 
CCCACGGCAG CCCTGAGCCC TGAGCCCCAA GATAGCAAAG AAGACGTGAA GAAGTTTGCT 
TTTGACGCTT GTAATGACGT CCCTGCGCCT CCTAAAGAGT CCTCAGCCAG TGAGGGCGCT 
GATCAGAAAA TGAGTTCTGT GGAGGAGGAC TCGGATACAA AAAGGCTTTC CCGAGAAGAG 
AAGGGTCGCA GCAGCTGTGG TAGGAATTTC TGGGTTAGTG GCCTTTCGTC TACAACCAGA 
GCCACGGATC TGAAGAACCT GTTTAGCAGA TATGGGAAGG TGGTGGGTGC TAAGGTTGTG 
ACGAACGCCC GGAGTCCTGG AGCTCGCTGT TATGGCTTTG TCACTATGTC CACAGCTGAA 
GAGGCAACAA AATGCATTAG CCATTTGCAC AAGACTGAGC TGCATGGCAA GATGATCTCA 
GTGGAAAAGG CCAAAAGTGA GCCCACTGGC AAAAGAGTGC CTGACAGGCG AGATGGGGAC 
AGCAAGAAGG AGAAGGCCAG CACCAGTGAC AGATCTGCAA ACCTTAAGAG GGAGGAGAAA 
GGTGAGAGAA AAGACGATGC CAAGAAGACA GATGATGGGA GCACAGAGAA GAGCAAAGAC 
GCAGATGACC AGAAACCTGG GCCTTCAGAG CGCTCGCGAA CGACCAAGTC AGGAAGCCGT 
GGCACTGAGC GCACTGTGGT GATGGACAAG TCCAAAGGTG TGCCTGTCAT CAGTGTGAAA 
ACATCTGGGT CTAAAGAGAG AGCCTCCAAA AGTCAGGACC GGAAGTCAGC CAGCCGGGAG 
AAGCGGTCTG TTGTGTCCTT TGACAAAGTT AAAGAGTCTC GAAAGTCCAG AGACTCAGAG 
TCTCGGAGGG AACGGGAGCG CAGTGAGCGG GAGCAGAGGC TGCAGGCCCA GTGGGAGCGG 
GAGGAGCGAG AGCGGCTGGA GATTGCTCGT GAGCGCTTGG CATTCCACCG CCATCGGCTA 
GAGCGTGAAC GCATGGAGCG GGAGCGGCTG GAGCGGGAGC GGATGCATGT AGAACAGGAG 
CGGAGGCGTG AGCAAGAGCG CATTCACCGA GAGCGAGAGG AGCTTCGGCG TCAGCAGGAG 
CTGCGCTATG AACAGGAGCG CCGGCCTGCT GTGCGCAGGC CCTATGAAGT GGATGGCCGG 
CGAGATGATG CCTATTGGCC AGAAGCTAAA CGGGCTGCTC TGGATGACCG TTACCACTCA 
GACTTCAGCC GCCAGGACCG CTTTCACGAC TTCGACCACA GAGACAGAGG CCGTTATCCC 
AACCATTCTG TGGATAGGAG AGAAGGTTCA CGGTCGATGA TGGGAGACAG AGAAGGACAG 
CATTACCCTG AACGCCATGG AGGACCTGAA CGCCATGGCC GGGACTCTCG AGATGGCTGG 
GGCTATGGTT CGAACAAGAG ATTGAGTGAA GGCCGGGGGC TGCCCCCTCC TCCCAGGGGC 
AGGCGTGACT GGGGGGAGCA TGGCCGGCGA CTTGAAGATG ACCGGGCATG GCAGGGCACG 
GCTGATGGAG GCATGATGGA GCGTGATCAT AAGAGGTGGC AAGGTGGTGA GAGGAGCATG 
TCTGGCCACT CGGGGCCTGG TCACATGATG AACCGTGGTG GCATGTCAGG GCGAGGCAGT 
TTTGCCCCAG GAGGAGCTTC TCGTGGCCAT GTGATCCCAC GTGGAGGGAT GCAGGCAGGC 
TTTGGAGGAC AGAGCCGGGG AAGCAGACCC AGTGATGCCC GCTTCACCCG CCGCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Safb ( NM_001374619.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001361548.1 Mus musculus scaffold attachment factor B (Safb), transcript variant 1, mRNA.

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CloneID	OMu53508
Clone ID Related Accession (Same CDS sequence)	XM_006524160.4 , XM_006524160.3 , NM_001374619.1
Accession Version	NM_001374619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-06
Organism	Mus musculus(house mouse)
Product	scaffold attachment factor B1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT485788.18. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGCGGAGA CTCTGTCGGG TTTAGGAGAC GCGAGTGCGG CGGGCGCGGC GGCCGTGAGC 
TCCGCGGCGT CAGAGACCGG GACGCGGCGG CTCAGCGACC TGCGGGTGAT CGACCTGCGG 
GCGGAGCTGA AGAAGCGGAA CCTCGACTCG AGCGGCAACA AGAGCGTGCT GATGGAGCGG 
CTGAAGAAGG CCATTGAAGA TGAAGGTGGG AATCCTGATG AAATTGAAGT CACCTCAGAA 
TGCAACAAGA AGATGCCTAA GAGGCCCAGC AAAGGGAGGA AACCAGAAGA CGAGGGTGTG 
GAGGACAACG GGCTCGAGGA GAATTCTGGG GATGGGCAGG AGGATGTGGA GACCAGCCTG 
GAGAACCTGC AAGACATGGA TATGATGGAC ATCAGTGTGC TGGATGAGGC TGACATTGAC 
AATGGCAGCG TGGCAGACTG TGTGGAGGAA GAGGAGGAGG CCACACTCCC TGAGGGCCTA 
GCAGACAGCA CTGAACTGGT AGAAGGAGAT TTGAAAGGGC TCCCAGAACA GCTGCAGGAG 
CATGCTATAG ATGACAAGGA CACCGTTAAC AATGTAGATA CGTCATCATC TGACTTCACC 
ATGCTGCAGG AGATGGAAGA GGCATCTTTG GAGCCAGAAA ATGAGAAAAT ACTCGACATT 
TTGGGGGAAA CTTGTAAATC TGAGCCAGTA AAAGAAGAAG GTTCGGAGCT GGAGCAGCCC 
TTTGCCCAGG CCACGAGTAG CGTGGGGCCA GACAGGAAGC TGGCGGAGGA AGAGGACCTA 
TTTGAGAGCT GCGGCCACCC GGAAGAGGAA GAGGAAGAGG AGGAAGAAGA TCAGGAAGAG 
GAGCAGGAGG AGGAGGGAGA TTTAGCTTTG GCCAGCAGCA GCAAGTCTGA GTCTCCAAGC 
ACTCGGTGTC AGTGGAGCGA GGCAGATGCC CCGTTAGCAG TAGTGAAAAG GGAGCCGGCA 
GATGCGCCTG GCGGAGGCAC TGGGATGGAC CGCGAGCCTG TAGGGCTAGA GGAGCCAGTT 
GAACAGAGTA GCACGGCTGC CCAGCTCCCG GAGGCCACCA GCCAGGAGCT GGTGCGAGCG 
CCCACGGCAG CCCTGAGCCC TGAGCCCCAA GATAGCAAAG AAGACGTGAA GAAGTTTGCT 
TTTGACGCTT GTAATGACGT CCCTGCGCCT CCTAAAGAGT CCTCAGCCAG TGAGGGCGCT 
GATCAGAAAA TGAGTTCTGT GGAGGAGGAC TCGGATACAA AAAGGCTTTC CCGAGAAGAG 
AAGGGTCGCA GCAGCTGTGG TAGGAATTTC TGGGTTAGTG GCCTTTCGTC TACAACCAGA 
GCCACGGATC TGAAGAACCT GTTTAGCAGA TATGGGAAGG TGGTGGGTGC TAAGGTTGTG 
ACGAACGCCC GGAGTCCTGG AGCTCGCTGT TATGGCTTTG TCACTATGTC CACAGCTGAA 
GAGGCAACAA AATGCATTAG CCATTTGCAC AAGACTGAGC TGCATGGCAA GATGATCTCA 
GTGGAAAAGG CCAAAAGTGA GCCCACTGGC AAAAGAGTGC CTGACAGGCG AGATGGGGAC 
AGCAAGAAGG AGAAGGCCAG CACCAGTGAC AGATCTGCAA ACCTTAAGAG GGAGGAGAAA 
GGTGAGAGAA AAGACGATGC CAAGAAGACA GATGATGGGA GCACAGAGAA GAGCAAAGAC 
GCAGATGACC AGAAACCTGG GCCTTCAGAG CGCTCGCGAA CGACCAAGTC AGGAAGCCGT 
GGCACTGAGC GCACTGTGGT GATGGACAAG TCCAAAGGTG TGCCTGTCAT CAGTGTGAAA 
ACATCTGGGT CTAAAGAGAG AGCCTCCAAA AGTCAGGACC GGAAGTCAGC CAGCCGGGAG 
AAGCGGTCTG TTGTGTCCTT TGACAAAGTT AAAGAGTCTC GAAAGTCCAG AGACTCAGAG 
TCTCGGAGGG AACGGGAGCG CAGTGAGCGG GAGCAGAGGC TGCAGGCCCA GTGGGAGCGG 
GAGGAGCGAG AGCGGCTGGA GATTGCTCGT GAGCGCTTGG CATTCCACCG CCATCGGCTA 
GAGCGTGAAC GCATGGAGCG GGAGCGGCTG GAGCGGGAGC GGATGCATGT AGAACAGGAG 
CGGAGGCGTG AGCAAGAGCG CATTCACCGA GAGCGAGAGG AGCTTCGGCG TCAGCAGGAG 
CTGCGCTATG AACAGGAGCG CCGGCCTGCT GTGCGCAGGC CCTATGAAGT GGATGGCCGG 
CGAGATGATG CCTATTGGCC AGAAGCTAAA CGGGCTGCTC TGGATGACCG TTACCACTCA 
GACTTCAGCC GCCAGGACCG CTTTCACGAC TTCGACCACA GAGACAGAGG CCGTTATCCC 
AACCATTCTG TGGATAGGAG AGAAGGTTCA CGGTCGATGA TGGGAGACAG AGAAGGACAG 
CATTACCCTG AACGCCATGG AGGACCTGAA CGCCATGGCC GGGACTCTCG AGATGGCTGG 
GGCTATGGTT CGAACAAGAG ATTGAGTGAA GGCCGGGGGC TGCCCCCTCC TCCCAGGGGC 
AGGCGTGACT GGGGGGAGCA TGGCCGGCGA CTTGAAGATG ACCGGGCATG GCAGGGCACG 
GCTGATGGAG GCATGATGGA GCGTGATCAT AAGAGGTGGC AAGGTGGTGA GAGGAGCATG 
TCTGGCCACT CGGGGCCTGG TCACATGATG AACCGTGGTG GCATGTCAGG GCGAGGCAGT 
TTTGCCCCAG GAGGAGCTTC TCGTGGCCAT GTGATCCCAC GTGGAGGGAT GCAGGCAGGC 
TTTGGAGGAC AGAGCCGGGG AAGCAGACCC AGTGATGCCC GCTTCACCCG CCGCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361548.1
CDS	181..3000
Translation	MAETLSGLGDASAAGAAAVSSAASETGTRRLSDLRVIDLRAELKKRNLDSSGNKSVLMERLKKAIEDEGGNPDEIEVTSECNKKMPKRPSKGRKPEDEGVEDNGLEENSGDGQEDVETSLENLQDMDMMDISVLDEADIDNGSVADCVEEEEEATLPEGLADSTELVEGDLKGLPEQLQEHAIDDKDTVNNVDTSSSDFTMLQEMEEASLEPENEKILDILGETCKSEPVKEEGSELEQPFAQATSSVGPDRKLAEEEDLFESCGHPEEEEEEEEEDQEEEQEEEGDLALASSSKSESPSTRCQWSEADAPLAVVKREPADAPGGGTGMDREPVGLEEPVEQSSTAAQLPEATSQELVRAPTAALSPEPQDSKEDVKKFAFDACNDVPAPPKESSASEGADQKMSSVEEDSDTKRLSREEKGRSSCGRNFWVSGLSSTTRATDLKNLFSRYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCISHLHKTELHGKMISVEKAKSEPTGKRVPDRRDGDSKKEKASTSDRSANLKREEKGERKDDAKKTDDGSTEKSKDADDQKPGPSERSRTTKSGSRGTERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSASREKRSVVSFDKVKESRKSRDSESRRERERSEREQRLQAQWEREERERLEIARERLAFHRHRLERERMERERLERERMHVEQERRREQERIHREREELRRQQELRYEQERRPAVRRPYEVDGRRDDAYWPEAKRAALDDRYHSDFSRQDRFHDFDHRDRGRYPNHSVDRREGSRSMMGDREGQHYPERHGGPERHGRDSRDGWGYGSNKRLSEGRGLPPPPRGRRDWGEHGRRLEDDRAWQGTADGGMMERDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHVIPRGGMQAGFGGQSRGSRPSDARFTRRY

Target ORF information:

RefSeq Version	NM_001374619.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus scaffold attachment factor B (Safb), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374619.1

ORF Insert Sequence:

ATGGCGGAGA CTCTGTCGGG TTTAGGAGAC GCGAGTGCGG CGGGCGCGGC GGCCGTGAGC 
TCCGCGGCGT CAGAGACCGG GACGCGGCGG CTCAGCGACC TGCGGGTGAT CGACCTGCGG 
GCGGAGCTGA AGAAGCGGAA CCTCGACTCG AGCGGCAACA AGAGCGTGCT GATGGAGCGG 
CTGAAGAAGG CCATTGAAGA TGAAGGTGGG AATCCTGATG AAATTGAAGT CACCTCAGAA 
TGCAACAAGA AGATGCCTAA GAGGCCCAGC AAAGGGAGGA AACCAGAAGA CGAGGGTGTG 
GAGGACAACG GGCTCGAGGA GAATTCTGGG GATGGGCAGG AGGATGTGGA GACCAGCCTG 
GAGAACCTGC AAGACATGGA TATGATGGAC ATCAGTGTGC TGGATGAGGC TGACATTGAC 
AATGGCAGCG TGGCAGACTG TGTGGAGGAA GAGGAGGAGG CCACACTCCC TGAGGGCCTA 
GCAGACAGCA CTGAACTGGT AGAAGGAGAT TTGAAAGGGC TCCCAGAACA GCTGCAGGAG 
CATGCTATAG ATGACAAGGA CACCGTTAAC AATGTAGATA CGTCATCATC TGACTTCACC 
ATGCTGCAGG AGATGGAAGA GGCATCTTTG GAGCCAGAAA ATGAGAAAAT ACTCGACATT 
TTGGGGGAAA CTTGTAAATC TGAGCCAGTA AAAGAAGAAG GTTCGGAGCT GGAGCAGCCC 
TTTGCCCAGG CCACGAGTAG CGTGGGGCCA GACAGGAAGC TGGCGGAGGA AGAGGACCTA 
TTTGAGAGCT GCGGCCACCC GGAAGAGGAA GAGGAAGAGG AGGAAGAAGA TCAGGAAGAG 
GAGCAGGAGG AGGAGGGAGA TTTAGCTTTG GCCAGCAGCA GCAAGTCTGA GTCTCCAAGC 
ACTCGGTGTC AGTGGAGCGA GGCAGATGCC CCGTTAGCAG TAGTGAAAAG GGAGCCGGCA 
GATGCGCCTG GCGGAGGCAC TGGGATGGAC CGCGAGCCTG TAGGGCTAGA GGAGCCAGTT 
GAACAGAGTA GCACGGCTGC CCAGCTCCCG GAGGCCACCA GCCAGGAGCT GGTGCGAGCG 
CCCACGGCAG CCCTGAGCCC TGAGCCCCAA GATAGCAAAG AAGACGTGAA GAAGTTTGCT 
TTTGACGCTT GTAATGACGT CCCTGCGCCT CCTAAAGAGT CCTCAGCCAG TGAGGGCGCT 
GATCAGAAAA TGAGTTCTGT GGAGGAGGAC TCGGATACAA AAAGGCTTTC CCGAGAAGAG 
AAGGGTCGCA GCAGCTGTGG TAGGAATTTC TGGGTTAGTG GCCTTTCGTC TACAACCAGA 
GCCACGGATC TGAAGAACCT GTTTAGCAGA TATGGGAAGG TGGTGGGTGC TAAGGTTGTG 
ACGAACGCCC GGAGTCCTGG AGCTCGCTGT TATGGCTTTG TCACTATGTC CACAGCTGAA 
GAGGCAACAA AATGCATTAG CCATTTGCAC AAGACTGAGC TGCATGGCAA GATGATCTCA 
GTGGAAAAGG CCAAAAGTGA GCCCACTGGC AAAAGAGTGC CTGACAGGCG AGATGGGGAC 
AGCAAGAAGG AGAAGGCCAG CACCAGTGAC AGATCTGCAA ACCTTAAGAG GGAGGAGAAA 
GGTGAGAGAA AAGACGATGC CAAGAAGACA GATGATGGGA GCACAGAGAA GAGCAAAGAC 
GCAGATGACC AGAAACCTGG GCCTTCAGAG CGCTCGCGAA CGACCAAGTC AGGAAGCCGT 
GGCACTGAGC GCACTGTGGT GATGGACAAG TCCAAAGGTG TGCCTGTCAT CAGTGTGAAA 
ACATCTGGGT CTAAAGAGAG AGCCTCCAAA AGTCAGGACC GGAAGTCAGC CAGCCGGGAG 
AAGCGGTCTG TTGTGTCCTT TGACAAAGTT AAAGAGTCTC GAAAGTCCAG AGACTCAGAG 
TCTCGGAGGG AACGGGAGCG CAGTGAGCGG GAGCAGAGGC TGCAGGCCCA GTGGGAGCGG 
GAGGAGCGAG AGCGGCTGGA GATTGCTCGT GAGCGCTTGG CATTCCACCG CCATCGGCTA 
GAGCGTGAAC GCATGGAGCG GGAGCGGCTG GAGCGGGAGC GGATGCATGT AGAACAGGAG 
CGGAGGCGTG AGCAAGAGCG CATTCACCGA GAGCGAGAGG AGCTTCGGCG TCAGCAGGAG 
CTGCGCTATG AACAGGAGCG CCGGCCTGCT GTGCGCAGGC CCTATGAAGT GGATGGCCGG 
CGAGATGATG CCTATTGGCC AGAAGCTAAA CGGGCTGCTC TGGATGACCG TTACCACTCA 
GACTTCAGCC GCCAGGACCG CTTTCACGAC TTCGACCACA GAGACAGAGG CCGTTATCCC 
AACCATTCTG TGGATAGGAG AGAAGGTTCA CGGTCGATGA TGGGAGACAG AGAAGGACAG 
CATTACCCTG AACGCCATGG AGGACCTGAA CGCCATGGCC GGGACTCTCG AGATGGCTGG 
GGCTATGGTT CGAACAAGAG ATTGAGTGAA GGCCGGGGGC TGCCCCCTCC TCCCAGGGGC 
AGGCGTGACT GGGGGGAGCA TGGCCGGCGA CTTGAAGATG ACCGGGCATG GCAGGGCACG 
GCTGATGGAG GCATGATGGA GCGTGATCAT AAGAGGTGGC AAGGTGGTGA GAGGAGCATG 
TCTGGCCACT CGGGGCCTGG TCACATGATG AACCGTGGTG GCATGTCAGG GCGAGGCAGT 
TTTGCCCCAG GAGGAGCTTC TCGTGGCCAT GTGATCCCAC GTGGAGGGAT GCAGGCAGGC 
TTTGGAGGAC AGAGCCGGGG AAGCAGACCC AGTGATGCCC GCTTCACCCG CCGCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Safb ( XM_006524160.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524223.1 Mus musculus scaffold attachment factor B (Safb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53508
Clone ID Related Accession (Same CDS sequence)	XM_006524160.4 , XM_006524160.3 , NM_001374619.1
Accession Version	XM_006524160.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	scaffold attachment factor B1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524160.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGA CTCTGTCGGG TTTAGGAGAC GCGAGTGCGG CGGGCGCGGC GGCCGTGAGC 
TCCGCGGCGT CAGAGACCGG GACGCGGCGG CTCAGCGACC TGCGGGTGAT CGACCTGCGG 
GCGGAGCTGA AGAAGCGGAA CCTCGACTCG AGCGGCAACA AGAGCGTGCT GATGGAGCGG 
CTGAAGAAGG CCATTGAAGA TGAAGGTGGG AATCCTGATG AAATTGAAGT CACCTCAGAA 
TGCAACAAGA AGATGCCTAA GAGGCCCAGC AAAGGGAGGA AACCAGAAGA CGAGGGTGTG 
GAGGACAACG GGCTCGAGGA GAATTCTGGG GATGGGCAGG AGGATGTGGA GACCAGCCTG 
GAGAACCTGC AAGACATGGA TATGATGGAC ATCAGTGTGC TGGATGAGGC TGACATTGAC 
AATGGCAGCG TGGCAGACTG TGTGGAGGAA GAGGAGGAGG CCACACTCCC TGAGGGCCTA 
GCAGACAGCA CTGAACTGGT AGAAGGAGAT TTGAAAGGGC TCCCAGAACA GCTGCAGGAG 
CATGCTATAG ATGACAAGGA CACCGTTAAC AATGTAGATA CGTCATCATC TGACTTCACC 
ATGCTGCAGG AGATGGAAGA GGCATCTTTG GAGCCAGAAA ATGAGAAAAT ACTCGACATT 
TTGGGGGAAA CTTGTAAATC TGAGCCAGTA AAAGAAGAAG GTTCGGAGCT GGAGCAGCCC 
TTTGCCCAGG CCACGAGTAG CGTGGGGCCA GACAGGAAGC TGGCGGAGGA AGAGGACCTA 
TTTGAGAGCT GCGGCCACCC GGAAGAGGAA GAGGAAGAGG AGGAAGAAGA TCAGGAAGAG 
GAGCAGGAGG AGGAGGGAGA TTTAGCTTTG GCCAGCAGCA GCAAGTCTGA GTCTCCAAGC 
ACTCGGTGTC AGTGGAGCGA GGCAGATGCC CCGTTAGCAG TAGTGAAAAG GGAGCCGGCA 
GATGCGCCTG GCGGAGGCAC TGGGATGGAC CGCGAGCCTG TAGGGCTAGA GGAGCCAGTT 
GAACAGAGTA GCACGGCTGC CCAGCTCCCG GAGGCCACCA GCCAGGAGCT GGTGCGAGCG 
CCCACGGCAG CCCTGAGCCC TGAGCCCCAA GATAGCAAAG AAGACGTGAA GAAGTTTGCT 
TTTGACGCTT GTAATGACGT CCCTGCGCCT CCTAAAGAGT CCTCAGCCAG TGAGGGCGCT 
GATCAGAAAA TGAGTTCTGT GGAGGAGGAC TCGGATACAA AAAGGCTTTC CCGAGAAGAG 
AAGGGTCGCA GCAGCTGTGG TAGGAATTTC TGGGTTAGTG GCCTTTCGTC TACAACCAGA 
GCCACGGATC TGAAGAACCT GTTTAGCAGA TATGGGAAGG TGGTGGGTGC TAAGGTTGTG 
ACGAACGCCC GGAGTCCTGG AGCTCGCTGT TATGGCTTTG TCACTATGTC CACAGCTGAA 
GAGGCAACAA AATGCATTAG CCATTTGCAC AAGACTGAGC TGCATGGCAA GATGATCTCA 
GTGGAAAAGG CCAAAAGTGA GCCCACTGGC AAAAGAGTGC CTGACAGGCG AGATGGGGAC 
AGCAAGAAGG AGAAGGCCAG CACCAGTGAC AGATCTGCAA ACCTTAAGAG GGAGGAGAAA 
GGTGAGAGAA AAGACGATGC CAAGAAGACA GATGATGGGA GCACAGAGAA GAGCAAAGAC 
GCAGATGACC AGAAACCTGG GCCTTCAGAG CGCTCGCGAA CGACCAAGTC AGGAAGCCGT 
GGCACTGAGC GCACTGTGGT GATGGACAAG TCCAAAGGTG TGCCTGTCAT CAGTGTGAAA 
ACATCTGGGT CTAAAGAGAG AGCCTCCAAA AGTCAGGACC GGAAGTCAGC CAGCCGGGAG 
AAGCGGTCTG TTGTGTCCTT TGACAAAGTT AAAGAGTCTC GAAAGTCCAG AGACTCAGAG 
TCTCGGAGGG AACGGGAGCG CAGTGAGCGG GAGCAGAGGC TGCAGGCCCA GTGGGAGCGG 
GAGGAGCGAG AGCGGCTGGA GATTGCTCGT GAGCGCTTGG CATTCCACCG CCATCGGCTA 
GAGCGTGAAC GCATGGAGCG GGAGCGGCTG GAGCGGGAGC GGATGCATGT AGAACAGGAG 
CGGAGGCGTG AGCAAGAGCG CATTCACCGA GAGCGAGAGG AGCTTCGGCG TCAGCAGGAG 
CTGCGCTATG AACAGGAGCG CCGGCCTGCT GTGCGCAGGC CCTATGAAGT GGATGGCCGG 
CGAGATGATG CCTATTGGCC AGAAGCTAAA CGGGCTGCTC TGGATGACCG TTACCACTCA 
GACTTCAGCC GCCAGGACCG CTTTCACGAC TTCGACCACA GAGACAGAGG CCGTTATCCC 
AACCATTCTG TGGATAGGAG AGAAGGTTCA CGGTCGATGA TGGGAGACAG AGAAGGACAG 
CATTACCCTG AACGCCATGG AGGACCTGAA CGCCATGGCC GGGACTCTCG AGATGGCTGG 
GGCTATGGTT CGAACAAGAG ATTGAGTGAA GGCCGGGGGC TGCCCCCTCC TCCCAGGGGC 
AGGCGTGACT GGGGGGAGCA TGGCCGGCGA CTTGAAGATG ACCGGGCATG GCAGGGCACG 
GCTGATGGAG GCATGATGGA GCGTGATCAT AAGAGGTGGC AAGGTGGTGA GAGGAGCATG 
TCTGGCCACT CGGGGCCTGG TCACATGATG AACCGTGGTG GCATGTCAGG GCGAGGCAGT 
TTTGCCCCAG GAGGAGCTTC TCGTGGCCAT GTGATCCCAC GTGGAGGGAT GCAGGCAGGC 
TTTGGAGGAC AGAGCCGGGG AAGCAGACCC AGTGATGCCC GCTTCACCCG CCGCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524223.1
CDS	425..3244
Translation	MAETLSGLGDASAAGAAAVSSAASETGTRRLSDLRVIDLRAELKKRNLDSSGNKSVLMERLKKAIEDEGGNPDEIEVTSECNKKMPKRPSKGRKPEDEGVEDNGLEENSGDGQEDVETSLENLQDMDMMDISVLDEADIDNGSVADCVEEEEEATLPEGLADSTELVEGDLKGLPEQLQEHAIDDKDTVNNVDTSSSDFTMLQEMEEASLEPENEKILDILGETCKSEPVKEEGSELEQPFAQATSSVGPDRKLAEEEDLFESCGHPEEEEEEEEEDQEEEQEEEGDLALASSSKSESPSTRCQWSEADAPLAVVKREPADAPGGGTGMDREPVGLEEPVEQSSTAAQLPEATSQELVRAPTAALSPEPQDSKEDVKKFAFDACNDVPAPPKESSASEGADQKMSSVEEDSDTKRLSREEKGRSSCGRNFWVSGLSSTTRATDLKNLFSRYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCISHLHKTELHGKMISVEKAKSEPTGKRVPDRRDGDSKKEKASTSDRSANLKREEKGERKDDAKKTDDGSTEKSKDADDQKPGPSERSRTTKSGSRGTERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSASREKRSVVSFDKVKESRKSRDSESRRERERSEREQRLQAQWEREERERLEIARERLAFHRHRLERERMERERLERERMHVEQERRREQERIHREREELRRQQELRYEQERRPAVRRPYEVDGRRDDAYWPEAKRAALDDRYHSDFSRQDRFHDFDHRDRGRYPNHSVDRREGSRSMMGDREGQHYPERHGGPERHGRDSRDGWGYGSNKRLSEGRGLPPPPRGRRDWGEHGRRLEDDRAWQGTADGGMMERDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHVIPRGGMQAGFGGQSRGSRPSDARFTRRY

Target ORF information:

RefSeq Version	XM_006524160.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus scaffold attachment factor B (Safb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524160.4

ORF Insert Sequence:

ATGGCGGAGA CTCTGTCGGG TTTAGGAGAC GCGAGTGCGG CGGGCGCGGC GGCCGTGAGC 
TCCGCGGCGT CAGAGACCGG GACGCGGCGG CTCAGCGACC TGCGGGTGAT CGACCTGCGG 
GCGGAGCTGA AGAAGCGGAA CCTCGACTCG AGCGGCAACA AGAGCGTGCT GATGGAGCGG 
CTGAAGAAGG CCATTGAAGA TGAAGGTGGG AATCCTGATG AAATTGAAGT CACCTCAGAA 
TGCAACAAGA AGATGCCTAA GAGGCCCAGC AAAGGGAGGA AACCAGAAGA CGAGGGTGTG 
GAGGACAACG GGCTCGAGGA GAATTCTGGG GATGGGCAGG AGGATGTGGA GACCAGCCTG 
GAGAACCTGC AAGACATGGA TATGATGGAC ATCAGTGTGC TGGATGAGGC TGACATTGAC 
AATGGCAGCG TGGCAGACTG TGTGGAGGAA GAGGAGGAGG CCACACTCCC TGAGGGCCTA 
GCAGACAGCA CTGAACTGGT AGAAGGAGAT TTGAAAGGGC TCCCAGAACA GCTGCAGGAG 
CATGCTATAG ATGACAAGGA CACCGTTAAC AATGTAGATA CGTCATCATC TGACTTCACC 
ATGCTGCAGG AGATGGAAGA GGCATCTTTG GAGCCAGAAA ATGAGAAAAT ACTCGACATT 
TTGGGGGAAA CTTGTAAATC TGAGCCAGTA AAAGAAGAAG GTTCGGAGCT GGAGCAGCCC 
TTTGCCCAGG CCACGAGTAG CGTGGGGCCA GACAGGAAGC TGGCGGAGGA AGAGGACCTA 
TTTGAGAGCT GCGGCCACCC GGAAGAGGAA GAGGAAGAGG AGGAAGAAGA TCAGGAAGAG 
GAGCAGGAGG AGGAGGGAGA TTTAGCTTTG GCCAGCAGCA GCAAGTCTGA GTCTCCAAGC 
ACTCGGTGTC AGTGGAGCGA GGCAGATGCC CCGTTAGCAG TAGTGAAAAG GGAGCCGGCA 
GATGCGCCTG GCGGAGGCAC TGGGATGGAC CGCGAGCCTG TAGGGCTAGA GGAGCCAGTT 
GAACAGAGTA GCACGGCTGC CCAGCTCCCG GAGGCCACCA GCCAGGAGCT GGTGCGAGCG 
CCCACGGCAG CCCTGAGCCC TGAGCCCCAA GATAGCAAAG AAGACGTGAA GAAGTTTGCT 
TTTGACGCTT GTAATGACGT CCCTGCGCCT CCTAAAGAGT CCTCAGCCAG TGAGGGCGCT 
GATCAGAAAA TGAGTTCTGT GGAGGAGGAC TCGGATACAA AAAGGCTTTC CCGAGAAGAG 
AAGGGTCGCA GCAGCTGTGG TAGGAATTTC TGGGTTAGTG GCCTTTCGTC TACAACCAGA 
GCCACGGATC TGAAGAACCT GTTTAGCAGA TATGGGAAGG TGGTGGGTGC TAAGGTTGTG 
ACGAACGCCC GGAGTCCTGG AGCTCGCTGT TATGGCTTTG TCACTATGTC CACAGCTGAA 
GAGGCAACAA AATGCATTAG CCATTTGCAC AAGACTGAGC TGCATGGCAA GATGATCTCA 
GTGGAAAAGG CCAAAAGTGA GCCCACTGGC AAAAGAGTGC CTGACAGGCG AGATGGGGAC 
AGCAAGAAGG AGAAGGCCAG CACCAGTGAC AGATCTGCAA ACCTTAAGAG GGAGGAGAAA 
GGTGAGAGAA AAGACGATGC CAAGAAGACA GATGATGGGA GCACAGAGAA GAGCAAAGAC 
GCAGATGACC AGAAACCTGG GCCTTCAGAG CGCTCGCGAA CGACCAAGTC AGGAAGCCGT 
GGCACTGAGC GCACTGTGGT GATGGACAAG TCCAAAGGTG TGCCTGTCAT CAGTGTGAAA 
ACATCTGGGT CTAAAGAGAG AGCCTCCAAA AGTCAGGACC GGAAGTCAGC CAGCCGGGAG 
AAGCGGTCTG TTGTGTCCTT TGACAAAGTT AAAGAGTCTC GAAAGTCCAG AGACTCAGAG 
TCTCGGAGGG AACGGGAGCG CAGTGAGCGG GAGCAGAGGC TGCAGGCCCA GTGGGAGCGG 
GAGGAGCGAG AGCGGCTGGA GATTGCTCGT GAGCGCTTGG CATTCCACCG CCATCGGCTA 
GAGCGTGAAC GCATGGAGCG GGAGCGGCTG GAGCGGGAGC GGATGCATGT AGAACAGGAG 
CGGAGGCGTG AGCAAGAGCG CATTCACCGA GAGCGAGAGG AGCTTCGGCG TCAGCAGGAG 
CTGCGCTATG AACAGGAGCG CCGGCCTGCT GTGCGCAGGC CCTATGAAGT GGATGGCCGG 
CGAGATGATG CCTATTGGCC AGAAGCTAAA CGGGCTGCTC TGGATGACCG TTACCACTCA 
GACTTCAGCC GCCAGGACCG CTTTCACGAC TTCGACCACA GAGACAGAGG CCGTTATCCC 
AACCATTCTG TGGATAGGAG AGAAGGTTCA CGGTCGATGA TGGGAGACAG AGAAGGACAG 
CATTACCCTG AACGCCATGG AGGACCTGAA CGCCATGGCC GGGACTCTCG AGATGGCTGG 
GGCTATGGTT CGAACAAGAG ATTGAGTGAA GGCCGGGGGC TGCCCCCTCC TCCCAGGGGC 
AGGCGTGACT GGGGGGAGCA TGGCCGGCGA CTTGAAGATG ACCGGGCATG GCAGGGCACG 
GCTGATGGAG GCATGATGGA GCGTGATCAT AAGAGGTGGC AAGGTGGTGA GAGGAGCATG 
TCTGGCCACT CGGGGCCTGG TCACATGATG AACCGTGGTG GCATGTCAGG GCGAGGCAGT 
TTTGCCCCAG GAGGAGCTTC TCGTGGCCAT GTGATCCCAC GTGGAGGGAT GCAGGCAGGC 
TTTGGAGGAC AGAGCCGGGG AAGCAGACCC AGTGATGCCC GCTTCACCCG CCGCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Safb ( XM_017317445.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172934.1 Mus musculus scaffold attachment factor B (Safb), transcript variant X2, mRNA.

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CloneID	OMu79576
Clone ID Related Accession (Same CDS sequence)	XM_017317445.1
Accession Version	XM_017317445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	scaffold attachment factor B1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGG GAATCCTGAT GAAATTGAAG TCACCTCAGA ATGCAACAAG AAGATGCCTA 
AGAGGCCCAG CAAAGATAGA TGACAAGGAC ACCGTTAACA ATGTAGATAC GTCATCATCT 
GACTTCACCA TGCTGCAGGA GATGGAAGAG GCATCTTTGG AGCCAGAAAA TGAGAAAATA 
CTCGACATTT TGGGGGAAAC TTGTAAATCT GAGCCAGTAA AAGAAGAAGG TTCGGAGCTG 
GAGCAGCCCT TTGCCCAGGC CACGAGTAGC GTGGGGCCAG ACAGGAAGCT GGCGGAGGAA 
GAGGACCTAT TTGAGAGCTG CGGCCACCCG GAAGAGGAAG AGGAAGAGGA GGAAGAAGAT 
CAGGAAGAGG AGCAGGAGGA GGAGGGAGAT TTAGCTTTGG CCAGCAGCAG CAAGTCTGAG 
TCTCCAAGCA CTCGGTGTCA GTGGAGCGAG GCAGATGCCC CGTTAGCAGT AGTGAAAAGG 
GAGCCGGCAG ATGCGCCTGG CGGAGGCACT GGGATGGACC GCGAGCCTGT AGGGCTAGAG 
GAGCCAGTTG AACAGAGTAG CACGGCTGCC CAGCTCCCGG AGGCCACCAG CCAGGAGCTG 
GTGCGAGCGC CCACGGCAGC CCTGAGCCCT GAGCCCCAAG ATAGCAAAGA AGACGTGAAG 
AAGTTTGCTT TTGACGCTTG TAATGACGTC CCTGCGCCTC CTAAAGAGTC CTCAGCCAGT 
GAGGGCGCTG ATCAGAAAAT GAGTTCTGTG GAGGAGGACT CGGATACAAA AAGGCTTTCC 
CGAGAAGAGA AGGGTCGCAG CAGCTGTGGT AGGAATTTCT GGGTTAGTGG CCTTTCGTCT 
ACAACCAGAG CCACGGATCT GAAGAACCTG TTTAGCAGAT ATGGGAAGGT GGTGGGTGCT 
AAGGTTGTGA CGAACGCCCG GAGTCCTGGA GCTCGCTGTT ATGGCTTTGT CACTATGTCC 
ACAGCTGAAG AGGCAACAAA ATGCATTAGC CATTTGCACA AGACTGAGCT GCATGGCAAG 
ATGATCTCAG TGGAAAAGGC CAAAAGTGAG CCCACTGGCA AAAGAGTGCC TGACAGGCGA 
GATGGGGACA GCAAGAAGGA GAAGGCCAGC ACCAGTGACA GATCTGCAAA CCTTAAGAGG 
GAGGAGAAAG GTGAGAGAAA AGACGATGCC AAGAAGACAG ATGATGGGAG CACAGAGAAG 
AGCAAAGACG CAGATGACCA GAAACCTGGG CCTTCAGAGC GCTCGCGAAC GACCAAGTCA 
GGAAGCCGTG GCACTGAGCG CACTGTGGTG ATGGACAAGT CCAAAGGTGT GCCTGTCATC 
AGTGTGAAAA CATCTGGGTC TAAAGAGAGA GCCTCCAAAA GTCAGGACCG GAAGTCAGCC 
AGCCGGGAGA AGCGGTCTGT TGTGTCCTTT GACAAAGTTA AAGAGTCTCG AAAGTCCAGA 
GACTCAGAGT CTCGGAGGGA ACGGGAGCGC AGTGAGCGGG AGCAGAGGCT GCAGGCCCAG 
TGGGAGCGGG AGGAGCGAGA GCGGCTGGAG ATTGCTCGTG AGCGCTTGGC ATTCCACCGC 
CATCGGCTAG AGCGTGAACG CATGGAGCGG GAGCGGCTGG AGCGGGAGCG GATGCATGTA 
GAACAGGAGC GGAGGCGTGA GCAAGAGCGC ATTCACCGAG AGCGAGAGGA GCTTCGGCGT 
CAGCAGGAGC TGCGCTATGA ACAGGAGCGC CGGCCTGCTG TGCGCAGGCC CTATGAAGTG 
GATGGCCGGC GAGATGATGC CTATTGGCCA GAAGCTAAAC GGGCTGCTCT GGATGACCGT 
TACCACTCAG ACTTCAGCCG CCAGGACCGC TTTCACGACT TCGACCACAG AGACAGAGGC 
CGTTATCCCA ACCATTCTGT GGATAGGAGA GAAGGTTCAC GGTCGATGAT GGGAGACAGA 
GAAGGACAGC ATTACCCTGA ACGCCATGGA GGACCTGAAC GCCATGGCCG GGACTCTCGA 
GATGGCTGGG GCTATGGTTC GAACAAGAGA TTGAGTGAAG GCCGGGGGCT GCCCCCTCCT 
CCCAGGGGCA GGCGTGACTG GGGGGAGCAT GGCCGGCGAC TTGAAGATGA CCGGGCATGG 
CAGGGCACGG CTGATGGAGG CATGATGGAG CGTGATCATA AGAGGTGGCA AGGTGGTGAG 
AGGAGCATGT CTGGCCACTC GGGGCCTGGT CACATGATGA ACCGTGGTGG CATGTCAGGG 
CGAGGCAGTT TTGCCCCAGG AGGAGCTTCT CGTGGCCATG TGATCCCACG TGGAGGGATG 
CAGGCAGGCT TTGGAGGACA GAGCCGGGGA AGCAGACCCA GTGATGCCCG CTTCACCCGC 
CGCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172934.1
CDS	1..2349
Translation	MKVGILMKLKSPQNATRRCLRGPAKIDDKDTVNNVDTSSSDFTMLQEMEEASLEPENEKILDILGETCKSEPVKEEGSELEQPFAQATSSVGPDRKLAEEEDLFESCGHPEEEEEEEEEDQEEEQEEEGDLALASSSKSESPSTRCQWSEADAPLAVVKREPADAPGGGTGMDREPVGLEEPVEQSSTAAQLPEATSQELVRAPTAALSPEPQDSKEDVKKFAFDACNDVPAPPKESSASEGADQKMSSVEEDSDTKRLSREEKGRSSCGRNFWVSGLSSTTRATDLKNLFSRYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCISHLHKTELHGKMISVEKAKSEPTGKRVPDRRDGDSKKEKASTSDRSANLKREEKGERKDDAKKTDDGSTEKSKDADDQKPGPSERSRTTKSGSRGTERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSASREKRSVVSFDKVKESRKSRDSESRRERERSEREQRLQAQWEREERERLEIARERLAFHRHRLERERMERERLERERMHVEQERRREQERIHREREELRRQQELRYEQERRPAVRRPYEVDGRRDDAYWPEAKRAALDDRYHSDFSRQDRFHDFDHRDRGRYPNHSVDRREGSRSMMGDREGQHYPERHGGPERHGRDSRDGWGYGSNKRLSEGRGLPPPPRGRRDWGEHGRRLEDDRAWQGTADGGMMERDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHVIPRGGMQAGFGGQSRGSRPSDARFTRRY

Target ORF information:

RefSeq Version	XM_017317445.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus scaffold attachment factor B (Safb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317445.1

ORF Insert Sequence:

ATGAAGGTGG GAATCCTGAT GAAATTGAAG TCACCTCAGA ATGCAACAAG AAGATGCCTA 
AGAGGCCCAG CAAAGATAGA TGACAAGGAC ACCGTTAACA ATGTAGATAC GTCATCATCT 
GACTTCACCA TGCTGCAGGA GATGGAAGAG GCATCTTTGG AGCCAGAAAA TGAGAAAATA 
CTCGACATTT TGGGGGAAAC TTGTAAATCT GAGCCAGTAA AAGAAGAAGG TTCGGAGCTG 
GAGCAGCCCT TTGCCCAGGC CACGAGTAGC GTGGGGCCAG ACAGGAAGCT GGCGGAGGAA 
GAGGACCTAT TTGAGAGCTG CGGCCACCCG GAAGAGGAAG AGGAAGAGGA GGAAGAAGAT 
CAGGAAGAGG AGCAGGAGGA GGAGGGAGAT TTAGCTTTGG CCAGCAGCAG CAAGTCTGAG 
TCTCCAAGCA CTCGGTGTCA GTGGAGCGAG GCAGATGCCC CGTTAGCAGT AGTGAAAAGG 
GAGCCGGCAG ATGCGCCTGG CGGAGGCACT GGGATGGACC GCGAGCCTGT AGGGCTAGAG 
GAGCCAGTTG AACAGAGTAG CACGGCTGCC CAGCTCCCGG AGGCCACCAG CCAGGAGCTG 
GTGCGAGCGC CCACGGCAGC CCTGAGCCCT GAGCCCCAAG ATAGCAAAGA AGACGTGAAG 
AAGTTTGCTT TTGACGCTTG TAATGACGTC CCTGCGCCTC CTAAAGAGTC CTCAGCCAGT 
GAGGGCGCTG ATCAGAAAAT GAGTTCTGTG GAGGAGGACT CGGATACAAA AAGGCTTTCC 
CGAGAAGAGA AGGGTCGCAG CAGCTGTGGT AGGAATTTCT GGGTTAGTGG CCTTTCGTCT 
ACAACCAGAG CCACGGATCT GAAGAACCTG TTTAGCAGAT ATGGGAAGGT GGTGGGTGCT 
AAGGTTGTGA CGAACGCCCG GAGTCCTGGA GCTCGCTGTT ATGGCTTTGT CACTATGTCC 
ACAGCTGAAG AGGCAACAAA ATGCATTAGC CATTTGCACA AGACTGAGCT GCATGGCAAG 
ATGATCTCAG TGGAAAAGGC CAAAAGTGAG CCCACTGGCA AAAGAGTGCC TGACAGGCGA 
GATGGGGACA GCAAGAAGGA GAAGGCCAGC ACCAGTGACA GATCTGCAAA CCTTAAGAGG 
GAGGAGAAAG GTGAGAGAAA AGACGATGCC AAGAAGACAG ATGATGGGAG CACAGAGAAG 
AGCAAAGACG CAGATGACCA GAAACCTGGG CCTTCAGAGC GCTCGCGAAC GACCAAGTCA 
GGAAGCCGTG GCACTGAGCG CACTGTGGTG ATGGACAAGT CCAAAGGTGT GCCTGTCATC 
AGTGTGAAAA CATCTGGGTC TAAAGAGAGA GCCTCCAAAA GTCAGGACCG GAAGTCAGCC 
AGCCGGGAGA AGCGGTCTGT TGTGTCCTTT GACAAAGTTA AAGAGTCTCG AAAGTCCAGA 
GACTCAGAGT CTCGGAGGGA ACGGGAGCGC AGTGAGCGGG AGCAGAGGCT GCAGGCCCAG 
TGGGAGCGGG AGGAGCGAGA GCGGCTGGAG ATTGCTCGTG AGCGCTTGGC ATTCCACCGC 
CATCGGCTAG AGCGTGAACG CATGGAGCGG GAGCGGCTGG AGCGGGAGCG GATGCATGTA 
GAACAGGAGC GGAGGCGTGA GCAAGAGCGC ATTCACCGAG AGCGAGAGGA GCTTCGGCGT 
CAGCAGGAGC TGCGCTATGA ACAGGAGCGC CGGCCTGCTG TGCGCAGGCC CTATGAAGTG 
GATGGCCGGC GAGATGATGC CTATTGGCCA GAAGCTAAAC GGGCTGCTCT GGATGACCGT 
TACCACTCAG ACTTCAGCCG CCAGGACCGC TTTCACGACT TCGACCACAG AGACAGAGGC 
CGTTATCCCA ACCATTCTGT GGATAGGAGA GAAGGTTCAC GGTCGATGAT GGGAGACAGA 
GAAGGACAGC ATTACCCTGA ACGCCATGGA GGACCTGAAC GCCATGGCCG GGACTCTCGA 
GATGGCTGGG GCTATGGTTC GAACAAGAGA TTGAGTGAAG GCCGGGGGCT GCCCCCTCCT 
CCCAGGGGCA GGCGTGACTG GGGGGAGCAT GGCCGGCGAC TTGAAGATGA CCGGGCATGG 
CAGGGCACGG CTGATGGAGG CATGATGGAG CGTGATCATA AGAGGTGGCA AGGTGGTGAG 
AGGAGCATGT CTGGCCACTC GGGGCCTGGT CACATGATGA ACCGTGGTGG CATGTCAGGG 
CGAGGCAGTT TTGCCCCAGG AGGAGCTTCT CGTGGCCATG TGATCCCACG TGGAGGGATG 
CAGGCAGGCT TTGGAGGACA GAGCCGGGGA AGCAGACCCA GTGATGCCCG CTTCACCCGC 
CGCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Scaffold attachment factor B2 (SAFB2)-null mice reveal non-redundant functions of SAFB2 compared with its paralog, SAFB1.
Disease models & mechanisms8(9)1121-7(2015 Sep)
Jiang S,Katz TA,Garee JP,DeMayo FJ,Lee AV,Oesterreich S

The Werner syndrome helicase protein is required for cell proliferation, immortalization, and tumorigenesis in Scaffold attachment factor B1 deficient mice.
Aging3(3)277-90(2011 Mar)
Lachapelle S,Oesterreich S,Lebel M

SAFB1, an RBMX-binding protein, is a newly identified regulator of hepatic SREBP-1c gene.
BMB reports42(4)232-7(2009 Apr)
Omura Y,Nishio Y,Takemoto T,Ikeuchi C,Sekine O,Morino K,Maeno Y,Obata T,Ugi S,Maegawa H,Kimura H,Kashiwagi A

Scaffold Attachment Factor B1 (SAFB1) heterozygosity does not influence Wnt-1 or DMBA-induced tumorigenesis.
Molecular cancer815(2009 Mar)
Kaipparettu BA,Dobrzycka KM,Britton O,Lee AV,Herron AJ,Li Y,Lewis MT,Medina D,Oesterreich S

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Safb cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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