Ubr2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ubr2
Entrez Gene ID	224826
Full Name	ubiquitin protein ligase E3 component n-recognin 2
Synonyms	9930021A08Rik,AI462103,AW540746,E130209G04Rik,mKIAA0349
General protein information	Preferred Names ubiquitin protein ligase E3 component n-recognin 2 Names E3 ubiquitin-protein ligase UBR2 N-recognin-2 RING-type E3 ubiquitin transferase UBR2 ubiquitin ligase E3 alpha-II ubiquitin-protein ligase E3-alpha-2 ubiquitin-protein ligase E3-alpha-II
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 C

mRNA and Protein(s)

mRNA	Protein	Name
XM_006524145.2	XP_006524208.1	E3 ubiquitin-protein ligase UBR2 isoform X2
XM_006524145.1	XP_006524208.1	E3 ubiquitin-protein ligase UBR2 isoform X1
NM_001177374.1	NP_001170845.1	E3 ubiquitin-protein ligase UBR2 isoform 2
XM_030249735.1	XP_030105595.1	E3 ubiquitin-protein ligase UBR2 isoform X1
NM_146078.3	NP_666190.2	E3 ubiquitin-protein ligase UBR2 isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ubr2	NP_001171542.1
Gallus gallus (chicken)	UBR2	XP_004935398.1
Drosophila melanogaster (fruit fly)	CG9086	NP_001245723.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000171	XP_310967.5
Caenorhabditis elegans (roundworm)	ubr-1	NP_491231.2
Xenopus tropicalis (tropical clawed frog)	ubr2	XP_002934736.1
	UBR2	XP_001088074.2
Bos taurus (cattle)	UBR2	NP_001179567.1
Danio rerio (zebrafish)	ubr2	XP_005156554.1
Mus musculus (house mouse)	Ubr2	NP_001170845.1
Homo sapiens (human)	UBR2	NP_056070.1
Pan troglodytes (chimpanzee)	UBR2	XP_001134707.1
Canis lupus familiaris (dog)	UBR2	XP_005627416.1

Gene Ontology
Bibliography

Related articles in PubMed

UBR2 Enriched in p53 Deficient Mouse Bone Marrow Mesenchymal Stem Cell-Exosome Promoted Gastric Cancer Progression via Wnt/?-Catenin Pathway.
Mao J, Liang Z, Zhang B, Yang H, Li X, Fu H, Zhang X, Yan Y, Xu W, Qian H
Stem cells (Dayton, Ohio)35(11)2267-2279(2017 Nov)

Mobilization of LINE-1 retrotransposons is restricted by
MacLennan M, Garc?a-Ca?adas M, Reichmann J, Khazina E, Wagner G, Playfoot CJ, Salvador-Palomeque C, Mann AR, Peressini P, Sanchez L, Dobie K, Read D, Hung CC, Eskeland R, Meehan RR, Weichenrieder O, Garc?a-P?rez JL, Adams IR
eLife6(2017 Aug)

Tex19.1 promotes Spo11-dependent meiotic recombination in mouse spermatocytes.
Crichton JH, Playfoot CJ, MacLennan M, Read D, Cooke HJ, Adams IR
PLoS genetics13(7)e1006904(2017 Jul)

Signaling mechanism of tumor cell-induced up-regulation of E3 ubiquitin ligase UBR2.
Zhang G, Lin RK, Kwon YT, Li YP
FASEB journal : official publication of the Federation of American Societies for Experimental Biology27(7)2893-901(2013 Jul)

The N-end rule pathway counteracts cell death by destroying proapoptotic protein fragments.
Piatkov KI, Brower CS, Varshavsky A
Proceedings of the National Academy of Sciences of the United States of America109(27)E1839-47(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data suggest that Tex19.1 and Ubr2 are required for mouse spermatocytes to accumulate sufficient Spo11-dependent recombination to ensure that the homology search is consistently successful, and reveal a hitherto unknown genetic pathway promoting meiotic recombination in mammals.
Title: Tex19.1 promotes Spo11-dependent meiotic recombination in mouse spermatocytes.

Results suggest that UBR2 up-regulation in cachectic muscle is mediated by the p38beta-C/EBPbeta signaling pathway responsible for the bulk of tumor-induced muscle proteolysis.
Title: Signaling mechanism of tumor cell-induced up-regulation of E3 ubiquitin ligase UBR2.

Results suggest that the binding of Ubr2 to Tex19.1 metabolically stabilizes Tex19.1 during spermatogenesis, revealing a new function for Ubr2 outside the conventional N-end rule pathway.
Title: The ubiquitin ligase Ubr2, a recognition E3 component of the N-end rule pathway, stabilizes Tex19.1 during spermatogenesis.

insufficiency in UBR2-dependent histone ubiquitination triggers a pachytene checkpoint system, providing a new insight into chromatin remodeling and gene expression regulation
Title: UBR2 mediates transcriptional silencing during spermatogenesis via histone ubiquitination.

UBR1-/-UBR2-/- embryos die at midgestation, with defects in neurogenesis and cardiovascular development.
Title: Impaired neurogenesis and cardiovascular development in mice lacking the E3 ubiquitin ligases UBR1 and UBR2 of the N-end rule pathway.

The following Ubr2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ubr2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu53497	XM_006524145.2 Latest version!	Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87174	XM_030249735.1 Latest version!	Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu12193	NM_146078.3 Latest version!	Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$951.30 $1359.00
OMu00250	NM_001177374.1 Latest version!	Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1287.30 $1839.00
View Old Accession Versions >>
OMu53497	XM_006524145.1	Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ubr2 ( XM_006524145.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524208.1 Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu53497
Clone ID Related Accession (Same CDS sequence)	XM_006524145.2 , XM_006524145.1
Accession Version	XM_006524145.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase UBR2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524145.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGA GAGATGTTCG ACAAGGACAT AGTGATGCTT CAGGTGTCTC CATGATGGAC 
CCAAACCACT TCCTGATGAT CATGCTCAGC CGCTTTGAAC TCTATCAGCT CTTCAGCACG 
CCTGACTATG GGAAGAGATT CAGTTCTGAG GTTACCCATA AGGACGTCGT TCAGCAGAAC 
AACACTCTGA TCGAAGAGAT GCTCTACCTC ATCATCATGC TTGTGGGAGA AAGATTCAAC 
CCTGGGGTTG GACAGGTGGC TGCCACAGAT GAAATCAAGA GGGAGATTAT CCATCAGTTG 
AGCATCAAGC CTATGGCTCA CAGTGAGCTG GTGAAGTCTC TGCCTGAAGA TGAGAACAAG 
GAGACCGGCA TGGAGAGCGT CATCGAGTCC GTTGCACATT TCAAGAAACC TGGGCTCACA 
GGGCGAGGCA TGTATGAGCT GAAGCCAGAG TGTGCCAAAG AGTTCAACCT GTATTTTTAT 
CATTTCTCCA GGGCAGAGCA GTCCAAGGCA GAGGAAGCTC AGCGGAAATT GAAAAGAGAA 
AATAAAGAAG ATACAGCACT CCCTCCTCCG GCTTTGCCAC CGTTCTGCCC TTTGTTCGCG 
AGTCTGGTTA ACATCTTGCA GTGTGACGTC ATGCTGTACA TCATGGGAAC GATCCTGCAG 
TGGGCTGTAG AGCATCACGG GTCTGCCTGG TCAGAGTCCA TGCTACAGAG GGTGCTGCAT 
TTGATCGGGA TGGCTCTCCA GGAAGAGAAG CACCACTTGG AGAACGCCGT GGAAGGGCAC 
GTGCAGACCT TCACCTTCAC ACAGAAGATT TCAAAGCCTG GTGATGCACC ACATAACTCC 
CCGAGCATCC TAGCTATGCT GGAGACCTTG CAGAACGCCC CCTCCCTGGA AGCCCACAAG 
GACATGATCA GGTGGTTGCT AAAGATGTTT AATGCAATTA AGAAGATAAG AGAGTGTTCA 
TCCAGCAGCC CTGTGGCCGA GGCGGAGGGA ACCATAATGG AGGAGAGCTC AAGAGACAAG 
GACAAAGCAG AGAGGAAAAG AAAAGCCGAG ATCGCCAGAC TGCGCCGGGA GAAGATCATG 
GCCCAGATGT CTGAGATGCA GCGGCACTTC ATTGACGAAA ACAAAGAGCT CTTCCAGCAG 
ACCCTAGAGC TGGACACCTC TGCCTCTGCC ACTCTTGACA GCAGCCCTCC CGTTTCAGAC 
GCAGCTCTTA CAGCACTGGG CCCAGCACAG ACACAGGTCC CTGAACCGAG ACAGTTTGTC 
ACCTGTATAT TATGTCAAGA GGAGCAAGAG GTGACTGTGG GAAGCAGGGC GATGGTCTTG 
GCAGCGTTTG TTCAGAGGTC AACGGTTCTG TCAAAAGACA GGACGAAAAC CATCGCGGAC 
CCAGAAAAAT ATGATCCATT ATTCATGCAC CCCGATCTGT CTTGTGGGAC ACACACTGGC 
AGCTGTGGGC ACGTTATGCA TGCCCATTGT TGGCAAAGGT ATTTTGATTC CGTTCAAGCC 
AAGGAGCAGC GAAGGCAGCA GCGGCTGCGC TTGCACACTA GCTACGATGT AGAGAATGGC 
GAGTTCCTCT GCCCGCTCTG TGAGTGCCTG AGCAACACGG TGATCCCCCT GCTGCTTCCT 
CCCAGGAGCA TCCTCAGCAG GAGGTTAAAT TTTTCAGACC AACCAGATCT GGCACAGTGG 
ACGAGAGCAG TAACACAGCA GATAAAGGTG GTCCAGATGC TGAGGAGAAA GCACAATGCT 
GCTGACACGT CTTCTTCAGA GGACACAGAA GCCATGAATA TAATACCGAT CCCCGAAGGC 
TTCAGGCCTG ATTTTTATCC TAGGAACCCA TATTCTGATA GCATAAAAGA AATGTTAACG 
ACATTTGGAA CGGCTGCTTA CAAGGTGGGA CTGAAGGTTC ATCCTAATGA AGGTGACCCC 
CGTGTGCCCA TCCTGTGCTG GGGGACCTGT GCATACACCA TCCAGAGCAT AGAAAGAATT 
TTGAGTGATG AGGAGAAGCC TGTTTTTGGA CCTCTGCCTT GTAGACTGGA CGACTGTCTC 
AGGTCGTTAA CACGGTTTGC AGCAGCACAT TGGACAGTGG CGTTACTTCC TGTGGTACAA 
GGACACTTCT GTAAACTCTT TGCATCCTTG GTGCCTAGTG ACAGCTATGA AGACCTCCCG 
TGCATACTAG ACATCGACAT GTTTCACTTG CTGGTGGGCC TGGTGCTCGC TTTCCCAGCT 
CTGCAGTGTC AGGATTTTTC AGGAAGCAGC CTGGCCACTG GGGACCTGCA CATCTTCCAC 
TTGGTTACCA TGGCACACAT CGTACAGATC TTACTTACCT CATGTACAGA AGAGAATGGC 
ATGGATCAAG AGAATCCCAC TGGGGAAGAA GAACTGGCCA TTCTCTCTTT GCACAAAACA 
CTTCACCAGT ATACTGGAAG TGCCTTGAAA GAAGCCCCCT CCGGCTGGCA CCTGTGGAGG 
AGCGTCCGGG CCGCCATCAT GCCTTTCCTC AAGTGCTCTG CTTTGTTTTT CCACTATTTA 
AATGGAGTCC CGGCCCCTCC AGACCTTCAA GTTTCTGGAA CAAGCCATTT TGAACACTTA 
TGTAACTACC TTTCCCTACC AACCAACCTC ATTCACCTTT TTCAAGAAAA CAGTGACATC 
ATGAACTCCC TGATTGAAAG TTGGTGCCAG AACAGTGAAG TTAAACGGTA TCTAAATGGC 
GAGAGAGGAG CGATAAGCTA CCCCAGAGGA GCTAACAAAC TGATAGACCT TCCAGAGGAT 
TACAGCAGCC TCATTAACCA AGCATCCAAT TTCTCGTGCC CCAAATCAGG TGGCGACAAG 
AGCAGAGCTC CTACTCTGTG CCTCGTGTGT GGGAGTCTCC TCTGCTCTCA GAGTTACTGC 
TGCCAAGCTG AGCTGGAGGG TGAGGACGTC GGAGCCTGCA CAGCACACAC CTACTCCTGC 
GGCTCCGGGG CCGGCATCTT CCTGAGAGTG CGGGAATGTC AGGTGCTATT TTTAGCTGGC 
AAAACCAAAG GATGTTTTTA TTCTCCTCCT TACCTTGACG ACTATGGAGA GACCGACCAG 
GGACTCAGAC GAGGAAATCC TTTACATTTA TGCCAAGAGC GGTTTCGAAA GATCCAGAAG 
CTCTGGCAGC AGCATAGTAT CACAGAGGAG ATCGGACACG CGCAGGAGGC TAACCAGACC 
CTGGTCGGAA TTGACTGGCA GCATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524208.1
CDS	1547..4756
Translation	MQARDVRQGHSDASGVSMMDPNHFLMIMLSRFELYQLFSTPDYGKRFSSEVTHKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVAATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIESVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRENKEDTALPPPALPPFCPLFASLVNILQCDVMLYIMGTILQWAVEHHGSAWSESMLQRVLHLIGMALQEEKHHLENAVEGHVQTFTFTQKISKPGDAPHNSPSILAMLETLQNAPSLEAHKDMIRWLLKMFNAIKKIRECSSSSPVAEAEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDTSASATLDSSPPVSDAALTALGPAQTQVPEPRQFVTCILCQEEQEVTVGSRAMVLAAFVQRSTVLSKDRTKTIADPEKYDPLFMHPDLSCGTHTGSCGHVMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRSILSRRLNFSDQPDLAQWTRAVTQQIKVVQMLRRKHNAADTSSSEDTEAMNIIPIPEGFRPDFYPRNPYSDSIKEMLTTFGTAAYKVGLKVHPNEGDPRVPILCWGTCAYTIQSIERILSDEEKPVFGPLPCRLDDCLRSLTRFAAAHWTVALLPVVQGHFCKLFASLVPSDSYEDLPCILDIDMFHLLVGLVLAFPALQCQDFSGSSLATGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPTGEEELAILSLHKTLHQYTGSALKEAPSGWHLWRSVRAAIMPFLKCSALFFHYLNGVPAPPDLQVSGTSHFEHLCNYLSLPTNLIHLFQENSDIMNSLIESWCQNSEVKRYLNGERGAISYPRGANKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGSLLCSQSYCCQAELEGEDVGACTAHTYSCGSGAGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCQERFRKIQKLWQQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_006524145.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524145.2

ORF Insert Sequence:

ATGCAGGCGA GAGATGTTCG ACAAGGACAT AGTGATGCTT CAGGTGTCTC CATGATGGAC 
CCAAACCACT TCCTGATGAT CATGCTCAGC CGCTTTGAAC TCTATCAGCT CTTCAGCACG 
CCTGACTATG GGAAGAGATT CAGTTCTGAG GTTACCCATA AGGACGTCGT TCAGCAGAAC 
AACACTCTGA TCGAAGAGAT GCTCTACCTC ATCATCATGC TTGTGGGAGA AAGATTCAAC 
CCTGGGGTTG GACAGGTGGC TGCCACAGAT GAAATCAAGA GGGAGATTAT CCATCAGTTG 
AGCATCAAGC CTATGGCTCA CAGTGAGCTG GTGAAGTCTC TGCCTGAAGA TGAGAACAAG 
GAGACCGGCA TGGAGAGCGT CATCGAGTCC GTTGCACATT TCAAGAAACC TGGGCTCACA 
GGGCGAGGCA TGTATGAGCT GAAGCCAGAG TGTGCCAAAG AGTTCAACCT GTATTTTTAT 
CATTTCTCCA GGGCAGAGCA GTCCAAGGCA GAGGAAGCTC AGCGGAAATT GAAAAGAGAA 
AATAAAGAAG ATACAGCACT CCCTCCTCCG GCTTTGCCAC CGTTCTGCCC TTTGTTCGCG 
AGTCTGGTTA ACATCTTGCA GTGTGACGTC ATGCTGTACA TCATGGGAAC GATCCTGCAG 
TGGGCTGTAG AGCATCACGG GTCTGCCTGG TCAGAGTCCA TGCTACAGAG GGTGCTGCAT 
TTGATCGGGA TGGCTCTCCA GGAAGAGAAG CACCACTTGG AGAACGCCGT GGAAGGGCAC 
GTGCAGACCT TCACCTTCAC ACAGAAGATT TCAAAGCCTG GTGATGCACC ACATAACTCC 
CCGAGCATCC TAGCTATGCT GGAGACCTTG CAGAACGCCC CCTCCCTGGA AGCCCACAAG 
GACATGATCA GGTGGTTGCT AAAGATGTTT AATGCAATTA AGAAGATAAG AGAGTGTTCA 
TCCAGCAGCC CTGTGGCCGA GGCGGAGGGA ACCATAATGG AGGAGAGCTC AAGAGACAAG 
GACAAAGCAG AGAGGAAAAG AAAAGCCGAG ATCGCCAGAC TGCGCCGGGA GAAGATCATG 
GCCCAGATGT CTGAGATGCA GCGGCACTTC ATTGACGAAA ACAAAGAGCT CTTCCAGCAG 
ACCCTAGAGC TGGACACCTC TGCCTCTGCC ACTCTTGACA GCAGCCCTCC CGTTTCAGAC 
GCAGCTCTTA CAGCACTGGG CCCAGCACAG ACACAGGTCC CTGAACCGAG ACAGTTTGTC 
ACCTGTATAT TATGTCAAGA GGAGCAAGAG GTGACTGTGG GAAGCAGGGC GATGGTCTTG 
GCAGCGTTTG TTCAGAGGTC AACGGTTCTG TCAAAAGACA GGACGAAAAC CATCGCGGAC 
CCAGAAAAAT ATGATCCATT ATTCATGCAC CCCGATCTGT CTTGTGGGAC ACACACTGGC 
AGCTGTGGGC ACGTTATGCA TGCCCATTGT TGGCAAAGGT ATTTTGATTC CGTTCAAGCC 
AAGGAGCAGC GAAGGCAGCA GCGGCTGCGC TTGCACACTA GCTACGATGT AGAGAATGGC 
GAGTTCCTCT GCCCGCTCTG TGAGTGCCTG AGCAACACGG TGATCCCCCT GCTGCTTCCT 
CCCAGGAGCA TCCTCAGCAG GAGGTTAAAT TTTTCAGACC AACCAGATCT GGCACAGTGG 
ACGAGAGCAG TAACACAGCA GATAAAGGTG GTCCAGATGC TGAGGAGAAA GCACAATGCT 
GCTGACACGT CTTCTTCAGA GGACACAGAA GCCATGAATA TAATACCGAT CCCCGAAGGC 
TTCAGGCCTG ATTTTTATCC TAGGAACCCA TATTCTGATA GCATAAAAGA AATGTTAACG 
ACATTTGGAA CGGCTGCTTA CAAGGTGGGA CTGAAGGTTC ATCCTAATGA AGGTGACCCC 
CGTGTGCCCA TCCTGTGCTG GGGGACCTGT GCATACACCA TCCAGAGCAT AGAAAGAATT 
TTGAGTGATG AGGAGAAGCC TGTTTTTGGA CCTCTGCCTT GTAGACTGGA CGACTGTCTC 
AGGTCGTTAA CACGGTTTGC AGCAGCACAT TGGACAGTGG CGTTACTTCC TGTGGTACAA 
GGACACTTCT GTAAACTCTT TGCATCCTTG GTGCCTAGTG ACAGCTATGA AGACCTCCCG 
TGCATACTAG ACATCGACAT GTTTCACTTG CTGGTGGGCC TGGTGCTCGC TTTCCCAGCT 
CTGCAGTGTC AGGATTTTTC AGGAAGCAGC CTGGCCACTG GGGACCTGCA CATCTTCCAC 
TTGGTTACCA TGGCACACAT CGTACAGATC TTACTTACCT CATGTACAGA AGAGAATGGC 
ATGGATCAAG AGAATCCCAC TGGGGAAGAA GAACTGGCCA TTCTCTCTTT GCACAAAACA 
CTTCACCAGT ATACTGGAAG TGCCTTGAAA GAAGCCCCCT CCGGCTGGCA CCTGTGGAGG 
AGCGTCCGGG CCGCCATCAT GCCTTTCCTC AAGTGCTCTG CTTTGTTTTT CCACTATTTA 
AATGGAGTCC CGGCCCCTCC AGACCTTCAA GTTTCTGGAA CAAGCCATTT TGAACACTTA 
TGTAACTACC TTTCCCTACC AACCAACCTC ATTCACCTTT TTCAAGAAAA CAGTGACATC 
ATGAACTCCC TGATTGAAAG TTGGTGCCAG AACAGTGAAG TTAAACGGTA TCTAAATGGC 
GAGAGAGGAG CGATAAGCTA CCCCAGAGGA GCTAACAAAC TGATAGACCT TCCAGAGGAT 
TACAGCAGCC TCATTAACCA AGCATCCAAT TTCTCGTGCC CCAAATCAGG TGGCGACAAG 
AGCAGAGCTC CTACTCTGTG CCTCGTGTGT GGGAGTCTCC TCTGCTCTCA GAGTTACTGC 
TGCCAAGCTG AGCTGGAGGG TGAGGACGTC GGAGCCTGCA CAGCACACAC CTACTCCTGC 
GGCTCCGGGG CCGGCATCTT CCTGAGAGTG CGGGAATGTC AGGTGCTATT TTTAGCTGGC 
AAAACCAAAG GATGTTTTTA TTCTCCTCCT TACCTTGACG ACTATGGAGA GACCGACCAG 
GGACTCAGAC GAGGAAATCC TTTACATTTA TGCCAAGAGC GGTTTCGAAA GATCCAGAAG 
CTCTGGCAGC AGCATAGTAT CACAGAGGAG ATCGGACACG CGCAGGAGGC TAACCAGACC 
CTGGTCGGAA TTGACTGGCA GCATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubr2 ( XM_030249735.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105595.1 Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu87174
Clone ID Related Accession (Same CDS sequence)	XM_030249735.1
Accession Version	XM_030249735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase UBR2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTCA CAGAAGAGAA CCTGATGACC GTTATCATTA AGGCTTTCAT GGACCATTTG 
AAACACAGAG ATGCCCAGGG CAGATTCCAG TTTGAACGCT ACACTGCCCT CCAAGCCTTC 
AAGTTCAGGA GAGTCCAGAG CCTCATCTTA GATCTCAAGT ATGTATTGAT TAGCAAACCA 
ACGGAGTGGT CAGATGAGCT GAGGCAGAAG TTCTTACAAG GGTTCGATGC CTTCTTGGAA 
TTACTGAAGT GCATGCAGGG AATGGACCCG ATCACGCGTC AGGTGGGACA GCACATTGAG 
ATGGAGCCAG AGTGGGAAGC AGCCTTCACA CTGCAGATGA AGCTGACACA CGTCATCTCA 
ATGGTGCAGG ACTGGTGTGC TCTGGACGAA AAAGTGTTAA TTGAAGCTTA CAAGAAATGC 
CTGGCTGTGC TGACACAGTG TCATGGCGGA TTTACTGATG GTGAACAGCC AATCACACTC 
AGTATTTGTG GACACTCGGT GGAAACCATC AGATACTGTG TTTCCCAAGA AAAAGTTAGC 
ATTCACCTCC CAATTTCTCG CTTGCTTGCA GGTTTGCATG TATTGTTAAG CAAAAGTGAA 
GTGGCATATA AATTTCCAGA GCTCCTACCT CTAAGTGAAC TGAGCCCACC CATGTTGATA 
GAACATCCTC TTAGATGTCT TGTCTTATGT GCTCAAGTGC ATGCTGGGAT GTGGAGAAGA 
AATGGCTTCT CTCTAGTAAA TCAGATCTAT TACTACCATA ATGTGAAATG CAGGCGAGAG 
ATGTTCGACA AGGACATAGT GATGCTTCAG ACAGGTGTCT CCATGATGGA CCCAAACCAC 
TTCCTGATGA TCATGCTCAG CCGCTTTGAA CTCTATCAGC TCTTCAGCAC GCCTGACTAT 
GGGAAGAGAT TCAGTTCTGA GGTTACCCAT AAGGACGTCG TTCAGCAGAA CAACACTCTG 
ATCGAAGAGA TGCTCTACCT CATCATCATG CTTGTGGGAG AAAGATTCAA CCCTGGGGTT 
GGACAGGTGG CTGCCACAGA TGAAATCAAG AGGGAGATTA TCCATCAGTT GAGCATCAAG 
CCTATGGCTC ACAGTGAGCT GGTGAAGTCT CTGCCTGAAG ATGAGAACAA GGAGACCGGC 
ATGGAGAGCG TCATCGAGTC CGTTGCACAT TTCAAGAAAC CTGGGCTCAC AGGGCGAGGC 
ATGTATGAGC TGAAGCCAGA GTGTGCCAAA GAGTTCAACC TGTATTTTTA TCATTTCTCC 
AGGGCAGAGC AGTCCAAGGC AGAGGAAGCT CAGCGGAAAT TGAAAAGAGA AAATAAAGAA 
GATACAGCAC TCCCTCCTCC GGCTTTGCCA CCGTTCTGCC CTTTGTTCGC GAGTCTGGTT 
AACATCTTGC AGTGTGACGT CATGCTGTAC ATCATGGGAA CGATCCTGCA GTGGGCTGTA 
GAGCATCACG GGTCTGCCTG GTCAGAGTCC ATGCTACAGA GGGTGCTGCA TTTGATCGGG 
ATGGCTCTCC AGGAAGAGAA GCACCACTTG GAGAACGCCG TGGAAGGGCA CGTGCAGACC 
TTCACCTTCA CACAGAAGAT TTCAAAGCCT GGTGATGCAC CACATAACTC CCCGAGCATC 
CTAGCTATGC TGGAGACCTT GCAGAACGCC CCCTCCCTGG AAGCCCACAA GGACATGATC 
AGGTGGTTGC TAAAGATGTT TAATGCAATT AAGAAGATAA GAGAGTGTTC ATCCAGCAGC 
CCTGTGGCCG AGGCGGAGGG AACCATAATG GAGGAGAGCT CAAGAGACAA GGACAAAGCA 
GAGAGGAAAA GAAAAGCCGA GATCGCCAGA CTGCGCCGGG AGAAGATCAT GGCCCAGATG 
TCTGAGATGC AGCGGCACTT CATTGACGAA AACAAAGAGC TCTTCCAGCA GACCCTAGAG 
CTGGACACCT CTGCCTCTGC CACTCTTGAC AGCAGCCCTC CCGTTTCAGA CGCAGCTCTT 
ACAGCACTGG GCCCAGCACA GACACAGGTC CCTGAACCGA GACAGTTTGT CACCTGTATA 
TTATGTCAAG AGGAGCAAGA GGTGACTGTG GGAAGCAGGG CGATGGTCTT GGCAGCGTTT 
GTTCAGAGGT CAACGGTTCT GTCAAAAGAC AGGACGAAAA CCATCGCGGA CCCAGAAAAA 
TATGATCCAT TATTCATGCA CCCCGATCTG TCTTGTGGGA CACACACTGG CAGCTGTGGG 
CACGTTATGC ATGCCCATTG TTGGCAAAGG TATTTTGATT CCGTTCAAGC CAAGGAGCAG 
CGAAGGCAGC AGCGGCTGCG CTTGCACACT AGCTACGATG TAGAGAATGG CGAGTTCCTC 
TGCCCGCTCT GTGAGTGCCT GAGCAACACG GTGATCCCCC TGCTGCTTCC TCCCAGGAGC 
ATCCTCAGCA GGAGGTTAAA TTTTTCAGAC CAACCAGATC TGGCACAGTG GACGAGAGCA 
GTAACACAGC AGATAAAGGT GGTCCAGATG CTGAGGAGAA AGCACAATGC TGCTGACACG 
TCTTCTTCAG AGGACACAGA AGCCATGAAT ATAATACCGA TCCCCGAAGG CTTCAGGCCT 
GATTTTTATC CTAGGAACCC ATATTCTGAT AGCATAAAAG AAATGTTAAC GACATTTGGA 
ACGGCTGCTT ACAAGGTGGG ACTGAAGGTT CATCCTAATG AAGGTGACCC CCGTGTGCCC 
ATCCTGTGCT GGGGGACCTG TGCATACACC ATCCAGAGCA TAGAAAGAAT TTTGAGTGAT 
GAGGAGAAGC CTGTTTTTGG ACCTCTGCCT TGTAGACTGG ACGACTGTCT CAGGTCGTTA 
ACACGGTTTG CAGCAGCACA TTGGACAGTG GCGTTACTTC CTGTGGTACA AGGACACTTC 
TGTAAACTCT TTGCATCCTT GGTGCCTAGT GACAGCTATG AAGACCTCCC GTGCATACTA 
GACATCGACA TGTTTCACTT GCTGGTGGGC CTGGTGCTCG CTTTCCCAGC TCTGCAGTGT 
CAGGATTTTT CAGGAAGCAG CCTGGCCACT GGGGACCTGC ACATCTTCCA CTTGGTTACC 
ATGGCACACA TCGTACAGAT CTTACTTACC TCATGTACAG AAGAGAATGG CATGGATCAA 
GAGAATCCCA CTGGGGAAGA AGAACTGGCC ATTCTCTCTT TGCACAAAAC ACTTCACCAG 
TATACTGGAA GTGCCTTGAA AGAAGCCCCC TCCGGCTGGC ACCTGTGGAG GAGCGTCCGG 
GCCGCCATCA TGCCTTTCCT CAAGTGCTCT GCTTTGTTTT TCCACTATTT AAATGGAGTC 
CCGGCCCCTC CAGACCTTCA AGTTTCTGGA ACAAGCCATT TTGAACACTT ATGTAACTAC 
CTTTCCCTAC CAACCAACCT CATTCACCTT TTTCAAGAAA ACAGTGACAT CATGAACTCC 
CTGATTGAAA GTTGGTGCCA GAACAGTGAA GTTAAACGGT ATCTAAATGG CGAGAGAGGA 
GCGATAAGCT ACCCCAGAGG AGCTAACAAA CTGATAGACC TTCCAGAGGA TTACAGCAGC 
CTCATTAACC AAGCATCCAA TTTCTCGTGC CCCAAATCAG GTGGCGACAA GAGCAGAGCT 
CCTACTCTGT GCCTCGTGTG TGGGAGTCTC CTCTGCTCTC AGAGTTACTG CTGCCAAGCT 
GAGCTGGAGG GTGAGGACGT CGGAGCCTGC ACAGCACACA CCTACTCCTG CGGCTCCGGG 
GCCGGCATCT TCCTGAGAGT GCGGGAATGT CAGGTGCTAT TTTTAGCTGG CAAAACCAAA 
GGATGTTTTT ATTCTCCTCC TTACCTTGAC GACTATGGAG AGACCGACCA GGGACTCAGA 
CGAGGAAATC CTTTACATTT ATGCCAAGAG CGGTTTCGAA AGATCCAGAA GCTCTGGCAG 
CAGCATAGTA TCACAGAGGA GATCGGACAC GCGCAGGAGG CTAACCAGAC CCTGGTCGGA 
ATTGACTGGC AGCATTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105595.1
CDS	1798..5778
Translation	MLLTEENLMTVIIKAFMDHLKHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDELRQKFLQGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMVQDWCALDEKVLIEAYKKCLAVLTQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPISRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQLFSTPDYGKRFSSEVTHKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVAATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIESVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRENKEDTALPPPALPPFCPLFASLVNILQCDVMLYIMGTILQWAVEHHGSAWSESMLQRVLHLIGMALQEEKHHLENAVEGHVQTFTFTQKISKPGDAPHNSPSILAMLETLQNAPSLEAHKDMIRWLLKMFNAIKKIRECSSSSPVAEAEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDTSASATLDSSPPVSDAALTALGPAQTQVPEPRQFVTCILCQEEQEVTVGSRAMVLAAFVQRSTVLSKDRTKTIADPEKYDPLFMHPDLSCGTHTGSCGHVMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRSILSRRLNFSDQPDLAQWTRAVTQQIKVVQMLRRKHNAADTSSSEDTEAMNIIPIPEGFRPDFYPRNPYSDSIKEMLTTFGTAAYKVGLKVHPNEGDPRVPILCWGTCAYTIQSIERILSDEEKPVFGPLPCRLDDCLRSLTRFAAAHWTVALLPVVQGHFCKLFASLVPSDSYEDLPCILDIDMFHLLVGLVLAFPALQCQDFSGSSLATGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPTGEEELAILSLHKTLHQYTGSALKEAPSGWHLWRSVRAAIMPFLKCSALFFHYLNGVPAPPDLQVSGTSHFEHLCNYLSLPTNLIHLFQENSDIMNSLIESWCQNSEVKRYLNGERGAISYPRGANKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGSLLCSQSYCCQAELEGEDVGACTAHTYSCGSGAGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCQERFRKIQKLWQQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_030249735.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249735.1

ORF Insert Sequence:

ATGCTCCTCA CAGAAGAGAA CCTGATGACC GTTATCATTA AGGCTTTCAT GGACCATTTG 
AAACACAGAG ATGCCCAGGG CAGATTCCAG TTTGAACGCT ACACTGCCCT CCAAGCCTTC 
AAGTTCAGGA GAGTCCAGAG CCTCATCTTA GATCTCAAGT ATGTATTGAT TAGCAAACCA 
ACGGAGTGGT CAGATGAGCT GAGGCAGAAG TTCTTACAAG GGTTCGATGC CTTCTTGGAA 
TTACTGAAGT GCATGCAGGG AATGGACCCG ATCACGCGTC AGGTGGGACA GCACATTGAG 
ATGGAGCCAG AGTGGGAAGC AGCCTTCACA CTGCAGATGA AGCTGACACA CGTCATCTCA 
ATGGTGCAGG ACTGGTGTGC TCTGGACGAA AAAGTGTTAA TTGAAGCTTA CAAGAAATGC 
CTGGCTGTGC TGACACAGTG TCATGGCGGA TTTACTGATG GTGAACAGCC AATCACACTC 
AGTATTTGTG GACACTCGGT GGAAACCATC AGATACTGTG TTTCCCAAGA AAAAGTTAGC 
ATTCACCTCC CAATTTCTCG CTTGCTTGCA GGTTTGCATG TATTGTTAAG CAAAAGTGAA 
GTGGCATATA AATTTCCAGA GCTCCTACCT CTAAGTGAAC TGAGCCCACC CATGTTGATA 
GAACATCCTC TTAGATGTCT TGTCTTATGT GCTCAAGTGC ATGCTGGGAT GTGGAGAAGA 
AATGGCTTCT CTCTAGTAAA TCAGATCTAT TACTACCATA ATGTGAAATG CAGGCGAGAG 
ATGTTCGACA AGGACATAGT GATGCTTCAG ACAGGTGTCT CCATGATGGA CCCAAACCAC 
TTCCTGATGA TCATGCTCAG CCGCTTTGAA CTCTATCAGC TCTTCAGCAC GCCTGACTAT 
GGGAAGAGAT TCAGTTCTGA GGTTACCCAT AAGGACGTCG TTCAGCAGAA CAACACTCTG 
ATCGAAGAGA TGCTCTACCT CATCATCATG CTTGTGGGAG AAAGATTCAA CCCTGGGGTT 
GGACAGGTGG CTGCCACAGA TGAAATCAAG AGGGAGATTA TCCATCAGTT GAGCATCAAG 
CCTATGGCTC ACAGTGAGCT GGTGAAGTCT CTGCCTGAAG ATGAGAACAA GGAGACCGGC 
ATGGAGAGCG TCATCGAGTC CGTTGCACAT TTCAAGAAAC CTGGGCTCAC AGGGCGAGGC 
ATGTATGAGC TGAAGCCAGA GTGTGCCAAA GAGTTCAACC TGTATTTTTA TCATTTCTCC 
AGGGCAGAGC AGTCCAAGGC AGAGGAAGCT CAGCGGAAAT TGAAAAGAGA AAATAAAGAA 
GATACAGCAC TCCCTCCTCC GGCTTTGCCA CCGTTCTGCC CTTTGTTCGC GAGTCTGGTT 
AACATCTTGC AGTGTGACGT CATGCTGTAC ATCATGGGAA CGATCCTGCA GTGGGCTGTA 
GAGCATCACG GGTCTGCCTG GTCAGAGTCC ATGCTACAGA GGGTGCTGCA TTTGATCGGG 
ATGGCTCTCC AGGAAGAGAA GCACCACTTG GAGAACGCCG TGGAAGGGCA CGTGCAGACC 
TTCACCTTCA CACAGAAGAT TTCAAAGCCT GGTGATGCAC CACATAACTC CCCGAGCATC 
CTAGCTATGC TGGAGACCTT GCAGAACGCC CCCTCCCTGG AAGCCCACAA GGACATGATC 
AGGTGGTTGC TAAAGATGTT TAATGCAATT AAGAAGATAA GAGAGTGTTC ATCCAGCAGC 
CCTGTGGCCG AGGCGGAGGG AACCATAATG GAGGAGAGCT CAAGAGACAA GGACAAAGCA 
GAGAGGAAAA GAAAAGCCGA GATCGCCAGA CTGCGCCGGG AGAAGATCAT GGCCCAGATG 
TCTGAGATGC AGCGGCACTT CATTGACGAA AACAAAGAGC TCTTCCAGCA GACCCTAGAG 
CTGGACACCT CTGCCTCTGC CACTCTTGAC AGCAGCCCTC CCGTTTCAGA CGCAGCTCTT 
ACAGCACTGG GCCCAGCACA GACACAGGTC CCTGAACCGA GACAGTTTGT CACCTGTATA 
TTATGTCAAG AGGAGCAAGA GGTGACTGTG GGAAGCAGGG CGATGGTCTT GGCAGCGTTT 
GTTCAGAGGT CAACGGTTCT GTCAAAAGAC AGGACGAAAA CCATCGCGGA CCCAGAAAAA 
TATGATCCAT TATTCATGCA CCCCGATCTG TCTTGTGGGA CACACACTGG CAGCTGTGGG 
CACGTTATGC ATGCCCATTG TTGGCAAAGG TATTTTGATT CCGTTCAAGC CAAGGAGCAG 
CGAAGGCAGC AGCGGCTGCG CTTGCACACT AGCTACGATG TAGAGAATGG CGAGTTCCTC 
TGCCCGCTCT GTGAGTGCCT GAGCAACACG GTGATCCCCC TGCTGCTTCC TCCCAGGAGC 
ATCCTCAGCA GGAGGTTAAA TTTTTCAGAC CAACCAGATC TGGCACAGTG GACGAGAGCA 
GTAACACAGC AGATAAAGGT GGTCCAGATG CTGAGGAGAA AGCACAATGC TGCTGACACG 
TCTTCTTCAG AGGACACAGA AGCCATGAAT ATAATACCGA TCCCCGAAGG CTTCAGGCCT 
GATTTTTATC CTAGGAACCC ATATTCTGAT AGCATAAAAG AAATGTTAAC GACATTTGGA 
ACGGCTGCTT ACAAGGTGGG ACTGAAGGTT CATCCTAATG AAGGTGACCC CCGTGTGCCC 
ATCCTGTGCT GGGGGACCTG TGCATACACC ATCCAGAGCA TAGAAAGAAT TTTGAGTGAT 
GAGGAGAAGC CTGTTTTTGG ACCTCTGCCT TGTAGACTGG ACGACTGTCT CAGGTCGTTA 
ACACGGTTTG CAGCAGCACA TTGGACAGTG GCGTTACTTC CTGTGGTACA AGGACACTTC 
TGTAAACTCT TTGCATCCTT GGTGCCTAGT GACAGCTATG AAGACCTCCC GTGCATACTA 
GACATCGACA TGTTTCACTT GCTGGTGGGC CTGGTGCTCG CTTTCCCAGC TCTGCAGTGT 
CAGGATTTTT CAGGAAGCAG CCTGGCCACT GGGGACCTGC ACATCTTCCA CTTGGTTACC 
ATGGCACACA TCGTACAGAT CTTACTTACC TCATGTACAG AAGAGAATGG CATGGATCAA 
GAGAATCCCA CTGGGGAAGA AGAACTGGCC ATTCTCTCTT TGCACAAAAC ACTTCACCAG 
TATACTGGAA GTGCCTTGAA AGAAGCCCCC TCCGGCTGGC ACCTGTGGAG GAGCGTCCGG 
GCCGCCATCA TGCCTTTCCT CAAGTGCTCT GCTTTGTTTT TCCACTATTT AAATGGAGTC 
CCGGCCCCTC CAGACCTTCA AGTTTCTGGA ACAAGCCATT TTGAACACTT ATGTAACTAC 
CTTTCCCTAC CAACCAACCT CATTCACCTT TTTCAAGAAA ACAGTGACAT CATGAACTCC 
CTGATTGAAA GTTGGTGCCA GAACAGTGAA GTTAAACGGT ATCTAAATGG CGAGAGAGGA 
GCGATAAGCT ACCCCAGAGG AGCTAACAAA CTGATAGACC TTCCAGAGGA TTACAGCAGC 
CTCATTAACC AAGCATCCAA TTTCTCGTGC CCCAAATCAG GTGGCGACAA GAGCAGAGCT 
CCTACTCTGT GCCTCGTGTG TGGGAGTCTC CTCTGCTCTC AGAGTTACTG CTGCCAAGCT 
GAGCTGGAGG GTGAGGACGT CGGAGCCTGC ACAGCACACA CCTACTCCTG CGGCTCCGGG 
GCCGGCATCT TCCTGAGAGT GCGGGAATGT CAGGTGCTAT TTTTAGCTGG CAAAACCAAA 
GGATGTTTTT ATTCTCCTCC TTACCTTGAC GACTATGGAG AGACCGACCA GGGACTCAGA 
CGAGGAAATC CTTTACATTT ATGCCAAGAG CGGTTTCGAA AGATCCAGAA GCTCTGGCAG 
CAGCATAGTA TCACAGAGGA GATCGGACAC GCGCAGGAGG CTAACCAGAC CCTGGTCGGA 
ATTGACTGGC AGCATTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubr2 ( XM_006524145.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524208.1 Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant X1, mRNA.

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CloneID	OMu53497
Clone ID Related Accession (Same CDS sequence)	XM_006524145.2 , XM_006524145.1
Accession Version	XM_006524145.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase UBR2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGA GAGATGTTCG ACAAGGACAT AGTGATGCTT CAGGTGTCTC CATGATGGAC 
CCAAACCACT TCCTGATGAT CATGCTCAGC CGCTTTGAAC TCTATCAGCT CTTCAGCACG 
CCTGACTATG GGAAGAGATT CAGTTCTGAG GTTACCCATA AGGACGTCGT TCAGCAGAAC 
AACACTCTGA TCGAAGAGAT GCTCTACCTC ATCATCATGC TTGTGGGAGA AAGATTCAAC 
CCTGGGGTTG GACAGGTGGC TGCCACAGAT GAAATCAAGA GGGAGATTAT CCATCAGTTG 
AGCATCAAGC CTATGGCTCA CAGTGAGCTG GTGAAGTCTC TGCCTGAAGA TGAGAACAAG 
GAGACCGGCA TGGAGAGCGT CATCGAGTCC GTTGCACATT TCAAGAAACC TGGGCTCACA 
GGGCGAGGCA TGTATGAGCT GAAGCCAGAG TGTGCCAAAG AGTTCAACCT GTATTTTTAT 
CATTTCTCCA GGGCAGAGCA GTCCAAGGCA GAGGAAGCTC AGCGGAAATT GAAAAGAGAA 
AATAAAGAAG ATACAGCACT CCCTCCTCCG GCTTTGCCAC CGTTCTGCCC TTTGTTCGCG 
AGTCTGGTTA ACATCTTGCA GTGTGACGTC ATGCTGTACA TCATGGGAAC GATCCTGCAG 
TGGGCTGTAG AGCATCACGG GTCTGCCTGG TCAGAGTCCA TGCTACAGAG GGTGCTGCAT 
TTGATCGGGA TGGCTCTCCA GGAAGAGAAG CACCACTTGG AGAACGCCGT GGAAGGGCAC 
GTGCAGACCT TCACCTTCAC ACAGAAGATT TCAAAGCCTG GTGATGCACC ACATAACTCC 
CCGAGCATCC TAGCTATGCT GGAGACCTTG CAGAACGCCC CCTCCCTGGA AGCCCACAAG 
GACATGATCA GGTGGTTGCT AAAGATGTTT AATGCAATTA AGAAGATAAG AGAGTGTTCA 
TCCAGCAGCC CTGTGGCCGA GGCGGAGGGA ACCATAATGG AGGAGAGCTC AAGAGACAAG 
GACAAAGCAG AGAGGAAAAG AAAAGCCGAG ATCGCCAGAC TGCGCCGGGA GAAGATCATG 
GCCCAGATGT CTGAGATGCA GCGGCACTTC ATTGACGAAA ACAAAGAGCT CTTCCAGCAG 
ACCCTAGAGC TGGACACCTC TGCCTCTGCC ACTCTTGACA GCAGCCCTCC CGTTTCAGAC 
GCAGCTCTTA CAGCACTGGG CCCAGCACAG ACACAGGTCC CTGAACCGAG ACAGTTTGTC 
ACCTGTATAT TATGTCAAGA GGAGCAAGAG GTGACTGTGG GAAGCAGGGC GATGGTCTTG 
GCAGCGTTTG TTCAGAGGTC AACGGTTCTG TCAAAAGACA GGACGAAAAC CATCGCGGAC 
CCAGAAAAAT ATGATCCATT ATTCATGCAC CCCGATCTGT CTTGTGGGAC ACACACTGGC 
AGCTGTGGGC ACGTTATGCA TGCCCATTGT TGGCAAAGGT ATTTTGATTC CGTTCAAGCC 
AAGGAGCAGC GAAGGCAGCA GCGGCTGCGC TTGCACACTA GCTACGATGT AGAGAATGGC 
GAGTTCCTCT GCCCGCTCTG TGAGTGCCTG AGCAACACGG TGATCCCCCT GCTGCTTCCT 
CCCAGGAGCA TCCTCAGCAG GAGGTTAAAT TTTTCAGACC AACCAGATCT GGCACAGTGG 
ACGAGAGCAG TAACACAGCA GATAAAGGTG GTCCAGATGC TGAGGAGAAA GCACAATGCT 
GCTGACACGT CTTCTTCAGA GGACACAGAA GCCATGAATA TAATACCGAT CCCCGAAGGC 
TTCAGGCCTG ATTTTTATCC TAGGAACCCA TATTCTGATA GCATAAAAGA AATGTTAACG 
ACATTTGGAA CGGCTGCTTA CAAGGTGGGA CTGAAGGTTC ATCCTAATGA AGGTGACCCC 
CGTGTGCCCA TCCTGTGCTG GGGGACCTGT GCATACACCA TCCAGAGCAT AGAAAGAATT 
TTGAGTGATG AGGAGAAGCC TGTTTTTGGA CCTCTGCCTT GTAGACTGGA CGACTGTCTC 
AGGTCGTTAA CACGGTTTGC AGCAGCACAT TGGACAGTGG CGTTACTTCC TGTGGTACAA 
GGACACTTCT GTAAACTCTT TGCATCCTTG GTGCCTAGTG ACAGCTATGA AGACCTCCCG 
TGCATACTAG ACATCGACAT GTTTCACTTG CTGGTGGGCC TGGTGCTCGC TTTCCCAGCT 
CTGCAGTGTC AGGATTTTTC AGGAAGCAGC CTGGCCACTG GGGACCTGCA CATCTTCCAC 
TTGGTTACCA TGGCACACAT CGTACAGATC TTACTTACCT CATGTACAGA AGAGAATGGC 
ATGGATCAAG AGAATCCCAC TGGGGAAGAA GAACTGGCCA TTCTCTCTTT GCACAAAACA 
CTTCACCAGT ATACTGGAAG TGCCTTGAAA GAAGCCCCCT CCGGCTGGCA CCTGTGGAGG 
AGCGTCCGGG CCGCCATCAT GCCTTTCCTC AAGTGCTCTG CTTTGTTTTT CCACTATTTA 
AATGGAGTCC CGGCCCCTCC AGACCTTCAA GTTTCTGGAA CAAGCCATTT TGAACACTTA 
TGTAACTACC TTTCCCTACC AACCAACCTC ATTCACCTTT TTCAAGAAAA CAGTGACATC 
ATGAACTCCC TGATTGAAAG TTGGTGCCAG AACAGTGAAG TTAAACGGTA TCTAAATGGC 
GAGAGAGGAG CGATAAGCTA CCCCAGAGGA GCTAACAAAC TGATAGACCT TCCAGAGGAT 
TACAGCAGCC TCATTAACCA AGCATCCAAT TTCTCGTGCC CCAAATCAGG TGGCGACAAG 
AGCAGAGCTC CTACTCTGTG CCTCGTGTGT GGGAGTCTCC TCTGCTCTCA GAGTTACTGC 
TGCCAAGCTG AGCTGGAGGG TGAGGACGTC GGAGCCTGCA CAGCACACAC CTACTCCTGC 
GGCTCCGGGG CCGGCATCTT CCTGAGAGTG CGGGAATGTC AGGTGCTATT TTTAGCTGGC 
AAAACCAAAG GATGTTTTTA TTCTCCTCCT TACCTTGACG ACTATGGAGA GACCGACCAG 
GGACTCAGAC GAGGAAATCC TTTACATTTA TGCCAAGAGC GGTTTCGAAA GATCCAGAAG 
CTCTGGCAGC AGCATAGTAT CACAGAGGAG ATCGGACACG CGCAGGAGGC TAACCAGACC 
CTGGTCGGAA TTGACTGGCA GCATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524208.1
CDS	381..3590
Translation	MQARDVRQGHSDASGVSMMDPNHFLMIMLSRFELYQLFSTPDYGKRFSSEVTHKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVAATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIESVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRENKEDTALPPPALPPFCPLFASLVNILQCDVMLYIMGTILQWAVEHHGSAWSESMLQRVLHLIGMALQEEKHHLENAVEGHVQTFTFTQKISKPGDAPHNSPSILAMLETLQNAPSLEAHKDMIRWLLKMFNAIKKIRECSSSSPVAEAEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDTSASATLDSSPPVSDAALTALGPAQTQVPEPRQFVTCILCQEEQEVTVGSRAMVLAAFVQRSTVLSKDRTKTIADPEKYDPLFMHPDLSCGTHTGSCGHVMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRSILSRRLNFSDQPDLAQWTRAVTQQIKVVQMLRRKHNAADTSSSEDTEAMNIIPIPEGFRPDFYPRNPYSDSIKEMLTTFGTAAYKVGLKVHPNEGDPRVPILCWGTCAYTIQSIERILSDEEKPVFGPLPCRLDDCLRSLTRFAAAHWTVALLPVVQGHFCKLFASLVPSDSYEDLPCILDIDMFHLLVGLVLAFPALQCQDFSGSSLATGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPTGEEELAILSLHKTLHQYTGSALKEAPSGWHLWRSVRAAIMPFLKCSALFFHYLNGVPAPPDLQVSGTSHFEHLCNYLSLPTNLIHLFQENSDIMNSLIESWCQNSEVKRYLNGERGAISYPRGANKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGSLLCSQSYCCQAELEGEDVGACTAHTYSCGSGAGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCQERFRKIQKLWQQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_006524145.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524145.1

ORF Insert Sequence:

ATGCAGGCGA GAGATGTTCG ACAAGGACAT AGTGATGCTT CAGGTGTCTC CATGATGGAC 
CCAAACCACT TCCTGATGAT CATGCTCAGC CGCTTTGAAC TCTATCAGCT CTTCAGCACG 
CCTGACTATG GGAAGAGATT CAGTTCTGAG GTTACCCATA AGGACGTCGT TCAGCAGAAC 
AACACTCTGA TCGAAGAGAT GCTCTACCTC ATCATCATGC TTGTGGGAGA AAGATTCAAC 
CCTGGGGTTG GACAGGTGGC TGCCACAGAT GAAATCAAGA GGGAGATTAT CCATCAGTTG 
AGCATCAAGC CTATGGCTCA CAGTGAGCTG GTGAAGTCTC TGCCTGAAGA TGAGAACAAG 
GAGACCGGCA TGGAGAGCGT CATCGAGTCC GTTGCACATT TCAAGAAACC TGGGCTCACA 
GGGCGAGGCA TGTATGAGCT GAAGCCAGAG TGTGCCAAAG AGTTCAACCT GTATTTTTAT 
CATTTCTCCA GGGCAGAGCA GTCCAAGGCA GAGGAAGCTC AGCGGAAATT GAAAAGAGAA 
AATAAAGAAG ATACAGCACT CCCTCCTCCG GCTTTGCCAC CGTTCTGCCC TTTGTTCGCG 
AGTCTGGTTA ACATCTTGCA GTGTGACGTC ATGCTGTACA TCATGGGAAC GATCCTGCAG 
TGGGCTGTAG AGCATCACGG GTCTGCCTGG TCAGAGTCCA TGCTACAGAG GGTGCTGCAT 
TTGATCGGGA TGGCTCTCCA GGAAGAGAAG CACCACTTGG AGAACGCCGT GGAAGGGCAC 
GTGCAGACCT TCACCTTCAC ACAGAAGATT TCAAAGCCTG GTGATGCACC ACATAACTCC 
CCGAGCATCC TAGCTATGCT GGAGACCTTG CAGAACGCCC CCTCCCTGGA AGCCCACAAG 
GACATGATCA GGTGGTTGCT AAAGATGTTT AATGCAATTA AGAAGATAAG AGAGTGTTCA 
TCCAGCAGCC CTGTGGCCGA GGCGGAGGGA ACCATAATGG AGGAGAGCTC AAGAGACAAG 
GACAAAGCAG AGAGGAAAAG AAAAGCCGAG ATCGCCAGAC TGCGCCGGGA GAAGATCATG 
GCCCAGATGT CTGAGATGCA GCGGCACTTC ATTGACGAAA ACAAAGAGCT CTTCCAGCAG 
ACCCTAGAGC TGGACACCTC TGCCTCTGCC ACTCTTGACA GCAGCCCTCC CGTTTCAGAC 
GCAGCTCTTA CAGCACTGGG CCCAGCACAG ACACAGGTCC CTGAACCGAG ACAGTTTGTC 
ACCTGTATAT TATGTCAAGA GGAGCAAGAG GTGACTGTGG GAAGCAGGGC GATGGTCTTG 
GCAGCGTTTG TTCAGAGGTC AACGGTTCTG TCAAAAGACA GGACGAAAAC CATCGCGGAC 
CCAGAAAAAT ATGATCCATT ATTCATGCAC CCCGATCTGT CTTGTGGGAC ACACACTGGC 
AGCTGTGGGC ACGTTATGCA TGCCCATTGT TGGCAAAGGT ATTTTGATTC CGTTCAAGCC 
AAGGAGCAGC GAAGGCAGCA GCGGCTGCGC TTGCACACTA GCTACGATGT AGAGAATGGC 
GAGTTCCTCT GCCCGCTCTG TGAGTGCCTG AGCAACACGG TGATCCCCCT GCTGCTTCCT 
CCCAGGAGCA TCCTCAGCAG GAGGTTAAAT TTTTCAGACC AACCAGATCT GGCACAGTGG 
ACGAGAGCAG TAACACAGCA GATAAAGGTG GTCCAGATGC TGAGGAGAAA GCACAATGCT 
GCTGACACGT CTTCTTCAGA GGACACAGAA GCCATGAATA TAATACCGAT CCCCGAAGGC 
TTCAGGCCTG ATTTTTATCC TAGGAACCCA TATTCTGATA GCATAAAAGA AATGTTAACG 
ACATTTGGAA CGGCTGCTTA CAAGGTGGGA CTGAAGGTTC ATCCTAATGA AGGTGACCCC 
CGTGTGCCCA TCCTGTGCTG GGGGACCTGT GCATACACCA TCCAGAGCAT AGAAAGAATT 
TTGAGTGATG AGGAGAAGCC TGTTTTTGGA CCTCTGCCTT GTAGACTGGA CGACTGTCTC 
AGGTCGTTAA CACGGTTTGC AGCAGCACAT TGGACAGTGG CGTTACTTCC TGTGGTACAA 
GGACACTTCT GTAAACTCTT TGCATCCTTG GTGCCTAGTG ACAGCTATGA AGACCTCCCG 
TGCATACTAG ACATCGACAT GTTTCACTTG CTGGTGGGCC TGGTGCTCGC TTTCCCAGCT 
CTGCAGTGTC AGGATTTTTC AGGAAGCAGC CTGGCCACTG GGGACCTGCA CATCTTCCAC 
TTGGTTACCA TGGCACACAT CGTACAGATC TTACTTACCT CATGTACAGA AGAGAATGGC 
ATGGATCAAG AGAATCCCAC TGGGGAAGAA GAACTGGCCA TTCTCTCTTT GCACAAAACA 
CTTCACCAGT ATACTGGAAG TGCCTTGAAA GAAGCCCCCT CCGGCTGGCA CCTGTGGAGG 
AGCGTCCGGG CCGCCATCAT GCCTTTCCTC AAGTGCTCTG CTTTGTTTTT CCACTATTTA 
AATGGAGTCC CGGCCCCTCC AGACCTTCAA GTTTCTGGAA CAAGCCATTT TGAACACTTA 
TGTAACTACC TTTCCCTACC AACCAACCTC ATTCACCTTT TTCAAGAAAA CAGTGACATC 
ATGAACTCCC TGATTGAAAG TTGGTGCCAG AACAGTGAAG TTAAACGGTA TCTAAATGGC 
GAGAGAGGAG CGATAAGCTA CCCCAGAGGA GCTAACAAAC TGATAGACCT TCCAGAGGAT 
TACAGCAGCC TCATTAACCA AGCATCCAAT TTCTCGTGCC CCAAATCAGG TGGCGACAAG 
AGCAGAGCTC CTACTCTGTG CCTCGTGTGT GGGAGTCTCC TCTGCTCTCA GAGTTACTGC 
TGCCAAGCTG AGCTGGAGGG TGAGGACGTC GGAGCCTGCA CAGCACACAC CTACTCCTGC 
GGCTCCGGGG CCGGCATCTT CCTGAGAGTG CGGGAATGTC AGGTGCTATT TTTAGCTGGC 
AAAACCAAAG GATGTTTTTA TTCTCCTCCT TACCTTGACG ACTATGGAGA GACCGACCAG 
GGACTCAGAC GAGGAAATCC TTTACATTTA TGCCAAGAGC GGTTTCGAAA GATCCAGAAG 
CTCTGGCAGC AGCATAGTAT CACAGAGGAG ATCGGACACG CGCAGGAGGC TAACCAGACC 
CTGGTCGGAA TTGACTGGCA GCATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubr2 ( NM_146078.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666190.2 Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant 1, mRNA.

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CloneID	OMu12193
Clone ID Related Accession (Same CDS sequence)	NM_146078.3
Accession Version	NM_146078.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase UBR2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ126408.1 and BC075642.1. On Apr 7, 2010 this sequence version replaced NM_146078.2. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147445.1, AY061885.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG AGATGGAGCC CGAGGTGCAG GCCATCGACC GCAGTTTGCT GGAATGTTCT 
GCCGAAGAGA TCGCAGGGAG ATGGCTGCAA GCAACCGACC TCAACAGAGA AGTGTACCAG 
CATTTAGCCC ACTGTGTGCC CAAAATCTAC TGCCGGGGCC CTAACCCCTT CCCTCAGAAG 
GAAGACACGC TGGCACAGCA CATCCTGCTG GGACCGATGG AGTGGTACAT CTGCGCTGAA 
GACCCTGCGC TGGGATTTCC AAAGCTCGAG CAGGCAAACA AGCCTTCTCA CCTCTGTGGC 
CGAGTGTTTA AAGTGGGGGA ACCTACATAC TCCTGCAGAG ACTGTGCAGT TGACCCCACC 
TGTGTTTTAT GCATGGAGTG CTTCCTGGGA AGTATCCATA GAGACCATCG ATATAGGATG 
ACCACATCGG GAGGAGGGGG CTTCTGTGAC TGTGGTGACA CTGAGGCGTG GAAAGAGGGA 
CCTTACTGCC AGAAGCACAA GCTCAGCAGC TCTGAAGTTG TGGAGGAGGA GGATCCTCTT 
GTGCATCTAT CAGAAGATGT GATCGCCAGA ACTTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTAG ATATACTGAC CTGGGAAAAA GAAAGTGAAT TGCCTGAAGA CTTAGAAGTG 
GCAGAGAAGA GTGACACCTA CTACTGCATG CTGTTTAATG ATGAGGTTCA CACCTATGAG 
CAAGTCATTT ATACCCTTCA GAAAGCTGTG AACTGTACAC AGAAGGAAGC CATTGGCTTT 
GCAACTACAG TTGATCGAGA TGGCCGTAGG TCTGTCCGAT ATGGAGATTT CCAGTACTGT 
GATCAAGCAA AGACAGTCAT TGTGAGGAAC ACCAGCAGAC AGACCAAGCC GCTCAAAGTT 
CAAGTTATGC ACTCCTCCGT GGCTGCTCAT CAGAATTTTG GTTTGAAAGC TCTGTCGTGG 
CTGGGAAGTG TTATTGGATA CTCAGATGGC CTTCGCAGGA TTTTGTGTCA AGTTGGATTA 
CAAGAAGGTC CAGATGGCGA AAACTCTTCT CTGGTCGACA GACTGATGCT TAATGATTCC 
AAATTATGGA AAGGGGCTAG GAGTGTGTAT CACCAGTTGT TCATGAGCAG CCTGCTCATG 
GACCTCAAGT ATAAGAAGCT GTTCGCGCTT CGATTTGCTA AAAATTACCG GCAGTTGCAG 
AGGGATTTTA TGGAGGATGA TCACGAGCGG GCAGTGTCGG TGACTGCTCT GTCTGTCCAG 
TTCTTCACCG CACCGACGCT GGCGCGAATG CTCCTCACAG AAGAGAACCT GATGACCGTT 
ATCATTAAGG CTTTCATGGA CCATTTGAAA CACAGAGATG CCCAGGGCAG ATTCCAGTTT 
GAACGCTACA CTGCCCTCCA AGCCTTCAAG TTCAGGAGAG TCCAGAGCCT CATCTTAGAT 
CTCAAGTATG TATTGATTAG CAAACCAACG GAGTGGTCAG ATGAGCTGAG GCAGAAGTTC 
TTACAAGGGT TCGATGCCTT CTTGGAATTA CTGAAGTGCA TGCAGGGAAT GGACCCGATC 
ACGCGTCAGG TGGGACAGCA CATTGAGATG GAGCCAGAGT GGGAAGCAGC CTTCACACTG 
CAGATGAAGC TGACACACGT CATCTCAATG GTGCAGGACT GGTGTGCTCT GGACGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTG GCTGTGCTGA CACAGTGTCA TGGCGGATTT 
ACTGATGGTG AACAGCCAAT CACACTCAGT ATTTGTGGAC ACTCGGTGGA AACCATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAA TTTCTCGCTT GCTTGCAGGT 
TTGCATGTAT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTGA GCCCACCCAT GTTGATAGAA CATCCTCTTA GATGTCTTGT CTTATGTGCT 
CAAGTGCATG CTGGGATGTG GAGAAGAAAT GGCTTCTCTC TAGTAAATCA GATCTATTAC 
TACCATAATG TGAAATGCAG GCGAGAGATG TTCGACAAGG ACATAGTGAT GCTTCAGACA 
GGTGTCTCCA TGATGGACCC AAACCACTTC CTGATGATCA TGCTCAGCCG CTTTGAACTC 
TATCAGCTCT TCAGCACGCC TGACTATGGG AAGAGATTCA GTTCTGAGGT TACCCATAAG 
GACGTCGTTC AGCAGAACAA CACTCTGATC GAAGAGATGC TCTACCTCAT CATCATGCTT 
GTGGGAGAAA GATTCAACCC TGGGGTTGGA CAGGTGGCTG CCACAGATGA AATCAAGAGG 
GAGATTATCC ATCAGTTGAG CATCAAGCCT ATGGCTCACA GTGAGCTGGT GAAGTCTCTG 
CCTGAAGATG AGAACAAGGA GACCGGCATG GAGAGCGTCA TCGAGTCCGT TGCACATTTC 
AAGAAACCTG GGCTCACAGG GCGAGGCATG TATGAGCTGA AGCCAGAGTG TGCCAAAGAG 
TTCAACCTGT ATTTTTATCA TTTCTCCAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAG 
CGGAAATTGA AAAGAGAAAA TAAAGAAGAT ACAGCACTCC CTCCTCCGGC TTTGCCACCG 
TTCTGCCCTT TGTTCGCGAG TCTGGTTAAC ATCTTGCAGT GTGACGTCAT GCTGTACATC 
ATGGGAACGA TCCTGCAGTG GGCTGTAGAG CATCACGGGT CTGCCTGGTC AGAGTCCATG 
CTACAGAGGG TGCTGCATTT GATCGGGATG GCTCTCCAGG AAGAGAAGCA CCACTTGGAG 
AACGCCGTGG AAGGGCACGT GCAGACCTTC ACCTTCACAC AGAAGATTTC AAAGCCTGGT 
GATGCACCAC ATAACTCCCC GAGCATCCTA GCTATGCTGG AGACCTTGCA GAACGCCCCC 
TCCCTGGAAG CCCACAAGGA CATGATCAGG TGGTTGCTAA AGATGTTTAA TGCAATTAAG 
AAGATAAGAG AGTGTTCATC CAGCAGCCCT GTGGCCGAGG CGGAGGGAAC CATAATGGAG 
GAGAGCTCAA GAGACAAGGA CAAAGCAGAG AGGAAAAGAA AAGCCGAGAT CGCCAGACTG 
CGCCGGGAGA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTCAT TGACGAAAAC 
AAAGAGCTCT TCCAGCAGAC CCTAGAGCTG GACACCTCTG CCTCTGCCAC TCTTGACAGC 
AGCCCTCCCG TTTCAGACGC AGCTCTTACA GCACTGGGCC CAGCACAGAC ACAGGTCCCT 
GAACCGAGAC AGTTTGTCAC CTGTATATTA TGTCAAGAGG AGCAAGAGGT GACTGTGGGA 
AGCAGGGCGA TGGTCTTGGC AGCGTTTGTT CAGAGGTCAA CGGTTCTGTC AAAAGACAGG 
ACGAAAACCA TCGCGGACCC AGAAAAATAT GATCCATTAT TCATGCACCC CGATCTGTCT 
TGTGGGACAC ACACTGGCAG CTGTGGGCAC GTTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCCAA GGAGCAGCGA AGGCAGCAGC GGCTGCGCTT GCACACTAGC 
TACGATGTAG AGAATGGCGA GTTCCTCTGC CCGCTCTGTG AGTGCCTGAG CAACACGGTG 
ATCCCCCTGC TGCTTCCTCC CAGGAGCATC CTCAGCAGGA GGTTAAATTT TTCAGACCAA 
CCAGATCTGG CACAGTGGAC GAGAGCAGTA ACACAGCAGA TAAAGGTGGT CCAGATGCTG 
AGGAGAAAGC ACAATGCTGC TGACACGTCT TCTTCAGAGG ACACAGAAGC CATGAATATA 
ATACCGATCC CCGAAGGCTT CAGGCCTGAT TTTTATCCTA GGAACCCATA TTCTGATAGC 
ATAAAAGAAA TGTTAACGAC ATTTGGAACG GCTGCTTACA AGGTGGGACT GAAGGTTCAT 
CCTAATGAAG GTGACCCCCG TGTGCCCATC CTGTGCTGGG GGACCTGTGC ATACACCATC 
CAGAGCATAG AAAGAATTTT GAGTGATGAG GAGAAGCCTG TTTTTGGACC TCTGCCTTGT 
AGACTGGACG ACTGTCTCAG GTCGTTAACA CGGTTTGCAG CAGCACATTG GACAGTGGCG 
TTACTTCCTG TGGTACAAGG ACACTTCTGT AAACTCTTTG CATCCTTGGT GCCTAGTGAC 
AGCTATGAAG ACCTCCCGTG CATACTAGAC ATCGACATGT TTCACTTGCT GGTGGGCCTG 
GTGCTCGCTT TCCCAGCTCT GCAGTGTCAG GATTTTTCAG GAAGCAGCCT GGCCACTGGG 
GACCTGCACA TCTTCCACTT GGTTACCATG GCACACATCG TACAGATCTT ACTTACCTCA 
TGTACAGAAG AGAATGGCAT GGATCAAGAG AATCCCACTG GGGAAGAAGA ACTGGCCATT 
CTCTCTTTGC ACAAAACACT TCACCAGTAT ACTGGAAGTG CCTTGAAAGA AGCCCCCTCC 
GGCTGGCACC TGTGGAGGAG CGTCCGGGCC GCCATCATGC CTTTCCTCAA GTGCTCTGCT 
TTGTTTTTCC ACTATTTAAA TGGAGTCCCG GCCCCTCCAG ACCTTCAAGT TTCTGGAACA 
AGCCATTTTG AACACTTATG TAACTACCTT TCCCTACCAA CCAACCTCAT TCACCTTTTT 
CAAGAAAACA GTGACATCAT GAACTCCCTG ATTGAAAGTT GGTGCCAGAA CAGTGAAGTT 
AAACGGTATC TAAATGGCGA GAGAGGAGCG ATAAGCTACC CCAGAGGAGC TAACAAACTG 
ATAGACCTTC CAGAGGATTA CAGCAGCCTC ATTAACCAAG CATCCAATTT CTCGTGCCCC 
AAATCAGGTG GCGACAAGAG CAGAGCTCCT ACTCTGTGCC TCGTGTGTGG GAGTCTCCTC 
TGCTCTCAGA GTTACTGCTG CCAAGCTGAG CTGGAGGGTG AGGACGTCGG AGCCTGCACA 
GCACACACCT ACTCCTGCGG CTCCGGGGCC GGCATCTTCC TGAGAGTGCG GGAATGTCAG 
GTGCTATTTT TAGCTGGCAA AACCAAAGGA TGTTTTTATT CTCCTCCTTA CCTTGACGAC 
TATGGAGAGA CCGACCAGGG ACTCAGACGA GGAAATCCTT TACATTTATG CCAAGAGCGG 
TTTCGAAAGA TCCAGAAGCT CTGGCAGCAG CATAGTATCA CAGAGGAGAT CGGACACGCG 
CAGGAGGCTA ACCAGACCCT GGTCGGAATT GACTGGCAGC ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666190.2
CDS	314..5581
Translation	MASEMEPEVQAIDRSLLECSAEEIAGRWLQATDLNREVYQHLAHCVPKIYCRGPNPFPQKEDTLAQHILLGPMEWYICAEDPALGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHKLSSSEVVEEEDPLVHLSEDVIARTYNIFAIMFRYAVDILTWEKESELPEDLEVAEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCDQAKTVIVRNTSRQTKPLKVQVMHSSVAAHQNFGLKALSWLGSVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFALRFAKNYRQLQRDFMEDDHERAVSVTALSVQFFTAPTLARMLLTEENLMTVIIKAFMDHLKHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDELRQKFLQGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMVQDWCALDEKVLIEAYKKCLAVLTQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPISRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQLFSTPDYGKRFSSEVTHKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVAATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIESVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRENKEDTALPPPALPPFCPLFASLVNILQCDVMLYIMGTILQWAVEHHGSAWSESMLQRVLHLIGMALQEEKHHLENAVEGHVQTFTFTQKISKPGDAPHNSPSILAMLETLQNAPSLEAHKDMIRWLLKMFNAIKKIRECSSSSPVAEAEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDTSASATLDSSPPVSDAALTALGPAQTQVPEPRQFVTCILCQEEQEVTVGSRAMVLAAFVQRSTVLSKDRTKTIADPEKYDPLFMHPDLSCGTHTGSCGHVMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRSILSRRLNFSDQPDLAQWTRAVTQQIKVVQMLRRKHNAADTSSSEDTEAMNIIPIPEGFRPDFYPRNPYSDSIKEMLTTFGTAAYKVGLKVHPNEGDPRVPILCWGTCAYTIQSIERILSDEEKPVFGPLPCRLDDCLRSLTRFAAAHWTVALLPVVQGHFCKLFASLVPSDSYEDLPCILDIDMFHLLVGLVLAFPALQCQDFSGSSLATGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPTGEEELAILSLHKTLHQYTGSALKEAPSGWHLWRSVRAAIMPFLKCSALFFHYLNGVPAPPDLQVSGTSHFEHLCNYLSLPTNLIHLFQENSDIMNSLIESWCQNSEVKRYLNGERGAISYPRGANKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGSLLCSQSYCCQAELEGEDVGACTAHTYSCGSGAGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCQERFRKIQKLWQQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	NM_146078.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146078.3

ORF Insert Sequence:

ATGGCGTCGG AGATGGAGCC CGAGGTGCAG GCCATCGACC GCAGTTTGCT GGAATGTTCT 
GCCGAAGAGA TCGCAGGGAG ATGGCTGCAA GCAACCGACC TCAACAGAGA AGTGTACCAG 
CATTTAGCCC ACTGTGTGCC CAAAATCTAC TGCCGGGGCC CTAACCCCTT CCCTCAGAAG 
GAAGACACGC TGGCACAGCA CATCCTGCTG GGACCGATGG AGTGGTACAT CTGCGCTGAA 
GACCCTGCGC TGGGATTTCC AAAGCTCGAG CAGGCAAACA AGCCTTCTCA CCTCTGTGGC 
CGAGTGTTTA AAGTGGGGGA ACCTACATAC TCCTGCAGAG ACTGTGCAGT TGACCCCACC 
TGTGTTTTAT GCATGGAGTG CTTCCTGGGA AGTATCCATA GAGACCATCG ATATAGGATG 
ACCACATCGG GAGGAGGGGG CTTCTGTGAC TGTGGTGACA CTGAGGCGTG GAAAGAGGGA 
CCTTACTGCC AGAAGCACAA GCTCAGCAGC TCTGAAGTTG TGGAGGAGGA GGATCCTCTT 
GTGCATCTAT CAGAAGATGT GATCGCCAGA ACTTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTAG ATATACTGAC CTGGGAAAAA GAAAGTGAAT TGCCTGAAGA CTTAGAAGTG 
GCAGAGAAGA GTGACACCTA CTACTGCATG CTGTTTAATG ATGAGGTTCA CACCTATGAG 
CAAGTCATTT ATACCCTTCA GAAAGCTGTG AACTGTACAC AGAAGGAAGC CATTGGCTTT 
GCAACTACAG TTGATCGAGA TGGCCGTAGG TCTGTCCGAT ATGGAGATTT CCAGTACTGT 
GATCAAGCAA AGACAGTCAT TGTGAGGAAC ACCAGCAGAC AGACCAAGCC GCTCAAAGTT 
CAAGTTATGC ACTCCTCCGT GGCTGCTCAT CAGAATTTTG GTTTGAAAGC TCTGTCGTGG 
CTGGGAAGTG TTATTGGATA CTCAGATGGC CTTCGCAGGA TTTTGTGTCA AGTTGGATTA 
CAAGAAGGTC CAGATGGCGA AAACTCTTCT CTGGTCGACA GACTGATGCT TAATGATTCC 
AAATTATGGA AAGGGGCTAG GAGTGTGTAT CACCAGTTGT TCATGAGCAG CCTGCTCATG 
GACCTCAAGT ATAAGAAGCT GTTCGCGCTT CGATTTGCTA AAAATTACCG GCAGTTGCAG 
AGGGATTTTA TGGAGGATGA TCACGAGCGG GCAGTGTCGG TGACTGCTCT GTCTGTCCAG 
TTCTTCACCG CACCGACGCT GGCGCGAATG CTCCTCACAG AAGAGAACCT GATGACCGTT 
ATCATTAAGG CTTTCATGGA CCATTTGAAA CACAGAGATG CCCAGGGCAG ATTCCAGTTT 
GAACGCTACA CTGCCCTCCA AGCCTTCAAG TTCAGGAGAG TCCAGAGCCT CATCTTAGAT 
CTCAAGTATG TATTGATTAG CAAACCAACG GAGTGGTCAG ATGAGCTGAG GCAGAAGTTC 
TTACAAGGGT TCGATGCCTT CTTGGAATTA CTGAAGTGCA TGCAGGGAAT GGACCCGATC 
ACGCGTCAGG TGGGACAGCA CATTGAGATG GAGCCAGAGT GGGAAGCAGC CTTCACACTG 
CAGATGAAGC TGACACACGT CATCTCAATG GTGCAGGACT GGTGTGCTCT GGACGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTG GCTGTGCTGA CACAGTGTCA TGGCGGATTT 
ACTGATGGTG AACAGCCAAT CACACTCAGT ATTTGTGGAC ACTCGGTGGA AACCATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAA TTTCTCGCTT GCTTGCAGGT 
TTGCATGTAT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTGA GCCCACCCAT GTTGATAGAA CATCCTCTTA GATGTCTTGT CTTATGTGCT 
CAAGTGCATG CTGGGATGTG GAGAAGAAAT GGCTTCTCTC TAGTAAATCA GATCTATTAC 
TACCATAATG TGAAATGCAG GCGAGAGATG TTCGACAAGG ACATAGTGAT GCTTCAGACA 
GGTGTCTCCA TGATGGACCC AAACCACTTC CTGATGATCA TGCTCAGCCG CTTTGAACTC 
TATCAGCTCT TCAGCACGCC TGACTATGGG AAGAGATTCA GTTCTGAGGT TACCCATAAG 
GACGTCGTTC AGCAGAACAA CACTCTGATC GAAGAGATGC TCTACCTCAT CATCATGCTT 
GTGGGAGAAA GATTCAACCC TGGGGTTGGA CAGGTGGCTG CCACAGATGA AATCAAGAGG 
GAGATTATCC ATCAGTTGAG CATCAAGCCT ATGGCTCACA GTGAGCTGGT GAAGTCTCTG 
CCTGAAGATG AGAACAAGGA GACCGGCATG GAGAGCGTCA TCGAGTCCGT TGCACATTTC 
AAGAAACCTG GGCTCACAGG GCGAGGCATG TATGAGCTGA AGCCAGAGTG TGCCAAAGAG 
TTCAACCTGT ATTTTTATCA TTTCTCCAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAG 
CGGAAATTGA AAAGAGAAAA TAAAGAAGAT ACAGCACTCC CTCCTCCGGC TTTGCCACCG 
TTCTGCCCTT TGTTCGCGAG TCTGGTTAAC ATCTTGCAGT GTGACGTCAT GCTGTACATC 
ATGGGAACGA TCCTGCAGTG GGCTGTAGAG CATCACGGGT CTGCCTGGTC AGAGTCCATG 
CTACAGAGGG TGCTGCATTT GATCGGGATG GCTCTCCAGG AAGAGAAGCA CCACTTGGAG 
AACGCCGTGG AAGGGCACGT GCAGACCTTC ACCTTCACAC AGAAGATTTC AAAGCCTGGT 
GATGCACCAC ATAACTCCCC GAGCATCCTA GCTATGCTGG AGACCTTGCA GAACGCCCCC 
TCCCTGGAAG CCCACAAGGA CATGATCAGG TGGTTGCTAA AGATGTTTAA TGCAATTAAG 
AAGATAAGAG AGTGTTCATC CAGCAGCCCT GTGGCCGAGG CGGAGGGAAC CATAATGGAG 
GAGAGCTCAA GAGACAAGGA CAAAGCAGAG AGGAAAAGAA AAGCCGAGAT CGCCAGACTG 
CGCCGGGAGA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTCAT TGACGAAAAC 
AAAGAGCTCT TCCAGCAGAC CCTAGAGCTG GACACCTCTG CCTCTGCCAC TCTTGACAGC 
AGCCCTCCCG TTTCAGACGC AGCTCTTACA GCACTGGGCC CAGCACAGAC ACAGGTCCCT 
GAACCGAGAC AGTTTGTCAC CTGTATATTA TGTCAAGAGG AGCAAGAGGT GACTGTGGGA 
AGCAGGGCGA TGGTCTTGGC AGCGTTTGTT CAGAGGTCAA CGGTTCTGTC AAAAGACAGG 
ACGAAAACCA TCGCGGACCC AGAAAAATAT GATCCATTAT TCATGCACCC CGATCTGTCT 
TGTGGGACAC ACACTGGCAG CTGTGGGCAC GTTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCCAA GGAGCAGCGA AGGCAGCAGC GGCTGCGCTT GCACACTAGC 
TACGATGTAG AGAATGGCGA GTTCCTCTGC CCGCTCTGTG AGTGCCTGAG CAACACGGTG 
ATCCCCCTGC TGCTTCCTCC CAGGAGCATC CTCAGCAGGA GGTTAAATTT TTCAGACCAA 
CCAGATCTGG CACAGTGGAC GAGAGCAGTA ACACAGCAGA TAAAGGTGGT CCAGATGCTG 
AGGAGAAAGC ACAATGCTGC TGACACGTCT TCTTCAGAGG ACACAGAAGC CATGAATATA 
ATACCGATCC CCGAAGGCTT CAGGCCTGAT TTTTATCCTA GGAACCCATA TTCTGATAGC 
ATAAAAGAAA TGTTAACGAC ATTTGGAACG GCTGCTTACA AGGTGGGACT GAAGGTTCAT 
CCTAATGAAG GTGACCCCCG TGTGCCCATC CTGTGCTGGG GGACCTGTGC ATACACCATC 
CAGAGCATAG AAAGAATTTT GAGTGATGAG GAGAAGCCTG TTTTTGGACC TCTGCCTTGT 
AGACTGGACG ACTGTCTCAG GTCGTTAACA CGGTTTGCAG CAGCACATTG GACAGTGGCG 
TTACTTCCTG TGGTACAAGG ACACTTCTGT AAACTCTTTG CATCCTTGGT GCCTAGTGAC 
AGCTATGAAG ACCTCCCGTG CATACTAGAC ATCGACATGT TTCACTTGCT GGTGGGCCTG 
GTGCTCGCTT TCCCAGCTCT GCAGTGTCAG GATTTTTCAG GAAGCAGCCT GGCCACTGGG 
GACCTGCACA TCTTCCACTT GGTTACCATG GCACACATCG TACAGATCTT ACTTACCTCA 
TGTACAGAAG AGAATGGCAT GGATCAAGAG AATCCCACTG GGGAAGAAGA ACTGGCCATT 
CTCTCTTTGC ACAAAACACT TCACCAGTAT ACTGGAAGTG CCTTGAAAGA AGCCCCCTCC 
GGCTGGCACC TGTGGAGGAG CGTCCGGGCC GCCATCATGC CTTTCCTCAA GTGCTCTGCT 
TTGTTTTTCC ACTATTTAAA TGGAGTCCCG GCCCCTCCAG ACCTTCAAGT TTCTGGAACA 
AGCCATTTTG AACACTTATG TAACTACCTT TCCCTACCAA CCAACCTCAT TCACCTTTTT 
CAAGAAAACA GTGACATCAT GAACTCCCTG ATTGAAAGTT GGTGCCAGAA CAGTGAAGTT 
AAACGGTATC TAAATGGCGA GAGAGGAGCG ATAAGCTACC CCAGAGGAGC TAACAAACTG 
ATAGACCTTC CAGAGGATTA CAGCAGCCTC ATTAACCAAG CATCCAATTT CTCGTGCCCC 
AAATCAGGTG GCGACAAGAG CAGAGCTCCT ACTCTGTGCC TCGTGTGTGG GAGTCTCCTC 
TGCTCTCAGA GTTACTGCTG CCAAGCTGAG CTGGAGGGTG AGGACGTCGG AGCCTGCACA 
GCACACACCT ACTCCTGCGG CTCCGGGGCC GGCATCTTCC TGAGAGTGCG GGAATGTCAG 
GTGCTATTTT TAGCTGGCAA AACCAAAGGA TGTTTTTATT CTCCTCCTTA CCTTGACGAC 
TATGGAGAGA CCGACCAGGG ACTCAGACGA GGAAATCCTT TACATTTATG CCAAGAGCGG 
TTTCGAAAGA TCCAGAAGCT CTGGCAGCAG CATAGTATCA CAGAGGAGAT CGGACACGCG 
CAGGAGGCTA ACCAGACCCT GGTCGGAATT GACTGGCAGC ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubr2 ( NM_001177374.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001170845.1 Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu00250
Clone ID Related Accession (Same CDS sequence)	NM_001177374.1
Accession Version	NM_001177374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase UBR2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143202.1 and BC075642.1. Transcript Variant: This variant (2) uses an alternative exon in the central coding region, compared to variant 1. The resulting isoform (2) is the same length but differs at an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK143202.1, AY280958.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG AGATGGAGCC CGAGGTGCAG GCCATCGACC GCAGTTTGCT GGAATGTTCT 
GCCGAAGAGA TCGCAGGGAG ATGGCTGCAA GCAACCGACC TCAACAGAGA AGTGTACCAG 
CATTTAGCCC ACTGTGTGCC CAAAATCTAC TGCCGGGGCC CTAACCCCTT CCCTCAGAAG 
GAAGACACGC TGGCACAGCA CATCCTGCTG GGACCGATGG AGTGGTACAT CTGCGCTGAA 
GACCCTGCGC TGGGATTTCC AAAGCTCGAG CAGGCAAACA AGCCTTCTCA CCTCTGTGGC 
CGAGTGTTTA AAGTGGGGGA ACCTACATAC TCCTGCAGAG ACTGTGCAGT TGACCCCACC 
TGTGTTTTAT GCATGGAGTG CTTCCTGGGA AGTATCCATA GAGACCATCG ATATAGGATG 
ACCACATCGG GAGGAGGGGG CTTCTGTGAC TGTGGTGACA CTGAGGCGTG GAAAGAGGGA 
CCTTACTGCC AGAAGCACAA GCTCAGCAGC TCTGAAGTTG TGGAGGAGGA GGATCCTCTT 
GTGCATCTAT CAGAAGATGT GATCGCCAGA ACTTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTAG ATATACTGAC CTGGGAAAAA GAAAGTGAAT TGCCTGAAGA CTTAGAAGTG 
GCAGAGAAGA GTGACACCTA CTACTGCATG CTGTTTAATG ATGAGGTTCA CACCTATGAG 
CAAGTCATTT ATACCCTTCA GAAAGCTGTG AACTGTACAC AGAAGGAAGC CATTGGCTTT 
GCAACTACAG TTGATCGAGA TGGCCGTAGG TCTGTCCGAT ATGGAGATTT CCAGTACTGT 
GATCAAGCAA AGACAGTCAT TGTGAGGAAC ACCAGCAGAC AGACCAAGCC GCTCAAAGTT 
CAAGTTATGC ACTCCTCCGT GGCTGCTCAT CAGAATTTTG GTTTGAAAGC TCTGTCGTGG 
CTGGGAAGTG TTATTGGATA CTCAGATGGC CTTCGCAGGA TTTTGTGTCA AGTTGGATTA 
CAAGAAGGTC CAGATGGCGA AAACTCTTCT CTGGTCGACA GACTGATGCT TAATGATTCC 
AAATTATGGA AAGGGGCTAG GAGTGTGTAT CACCAGTTGT TCATGAGCAG CCTGCTCATG 
GACCTCAAGT ATAAGAAGCT GTTCGCGCTT CGATTTGCTA AAAACTATGA GCGTTTGCAG 
AGAGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAG TCGCAGACCT CTCGGTTCAG 
ATATTCACAG TTCCTTCACT TGCGCGAATG CTCCTCACAG AAGAGAACCT GATGACCGTT 
ATCATTAAGG CTTTCATGGA CCATTTGAAA CACAGAGATG CCCAGGGCAG ATTCCAGTTT 
GAACGCTACA CTGCCCTCCA AGCCTTCAAG TTCAGGAGAG TCCAGAGCCT CATCTTAGAT 
CTCAAGTATG TATTGATTAG CAAACCAACG GAGTGGTCAG ATGAGCTGAG GCAGAAGTTC 
TTACAAGGGT TCGATGCCTT CTTGGAATTA CTGAAGTGCA TGCAGGGAAT GGACCCGATC 
ACGCGTCAGG TGGGACAGCA CATTGAGATG GAGCCAGAGT GGGAAGCAGC CTTCACACTG 
CAGATGAAGC TGACACACGT CATCTCAATG GTGCAGGACT GGTGTGCTCT GGACGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTG GCTGTGCTGA CACAGTGTCA TGGCGGATTT 
ACTGATGGTG AACAGCCAAT CACACTCAGT ATTTGTGGAC ACTCGGTGGA AACCATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAA TTTCTCGCTT GCTTGCAGGT 
TTGCATGTAT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTGA GCCCACCCAT GTTGATAGAA CATCCTCTTA GATGTCTTGT CTTATGTGCT 
CAAGTGCATG CTGGGATGTG GAGAAGAAAT GGCTTCTCTC TAGTAAATCA GATCTATTAC 
TACCATAATG TGAAATGCAG GCGAGAGATG TTCGACAAGG ACATAGTGAT GCTTCAGACA 
GGTGTCTCCA TGATGGACCC AAACCACTTC CTGATGATCA TGCTCAGCCG CTTTGAACTC 
TATCAGCTCT TCAGCACGCC TGACTATGGG AAGAGATTCA GTTCTGAGGT TACCCATAAG 
GACGTCGTTC AGCAGAACAA CACTCTGATC GAAGAGATGC TCTACCTCAT CATCATGCTT 
GTGGGAGAAA GATTCAACCC TGGGGTTGGA CAGGTGGCTG CCACAGATGA AATCAAGAGG 
GAGATTATCC ATCAGTTGAG CATCAAGCCT ATGGCTCACA GTGAGCTGGT GAAGTCTCTG 
CCTGAAGATG AGAACAAGGA GACCGGCATG GAGAGCGTCA TCGAGTCCGT TGCACATTTC 
AAGAAACCTG GGCTCACAGG GCGAGGCATG TATGAGCTGA AGCCAGAGTG TGCCAAAGAG 
TTCAACCTGT ATTTTTATCA TTTCTCCAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAG 
CGGAAATTGA AAAGAGAAAA TAAAGAAGAT ACAGCACTCC CTCCTCCGGC TTTGCCACCG 
TTCTGCCCTT TGTTCGCGAG TCTGGTTAAC ATCTTGCAGT GTGACGTCAT GCTGTACATC 
ATGGGAACGA TCCTGCAGTG GGCTGTAGAG CATCACGGGT CTGCCTGGTC AGAGTCCATG 
CTACAGAGGG TGCTGCATTT GATCGGGATG GCTCTCCAGG AAGAGAAGCA CCACTTGGAG 
AACGCCGTGG AAGGGCACGT GCAGACCTTC ACCTTCACAC AGAAGATTTC AAAGCCTGGT 
GATGCACCAC ATAACTCCCC GAGCATCCTA GCTATGCTGG AGACCTTGCA GAACGCCCCC 
TCCCTGGAAG CCCACAAGGA CATGATCAGG TGGTTGCTAA AGATGTTTAA TGCAATTAAG 
AAGATAAGAG AGTGTTCATC CAGCAGCCCT GTGGCCGAGG CGGAGGGAAC CATAATGGAG 
GAGAGCTCAA GAGACAAGGA CAAAGCAGAG AGGAAAAGAA AAGCCGAGAT CGCCAGACTG 
CGCCGGGAGA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTCAT TGACGAAAAC 
AAAGAGCTCT TCCAGCAGAC CCTAGAGCTG GACACCTCTG CCTCTGCCAC TCTTGACAGC 
AGCCCTCCCG TTTCAGACGC AGCTCTTACA GCACTGGGCC CAGCACAGAC ACAGGTCCCT 
GAACCGAGAC AGTTTGTCAC CTGTATATTA TGTCAAGAGG AGCAAGAGGT GACTGTGGGA 
AGCAGGGCGA TGGTCTTGGC AGCGTTTGTT CAGAGGTCAA CGGTTCTGTC AAAAGACAGG 
ACGAAAACCA TCGCGGACCC AGAAAAATAT GATCCATTAT TCATGCACCC CGATCTGTCT 
TGTGGGACAC ACACTGGCAG CTGTGGGCAC GTTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCCAA GGAGCAGCGA AGGCAGCAGC GGCTGCGCTT GCACACTAGC 
TACGATGTAG AGAATGGCGA GTTCCTCTGC CCGCTCTGTG AGTGCCTGAG CAACACGGTG 
ATCCCCCTGC TGCTTCCTCC CAGGAGCATC CTCAGCAGGA GGTTAAATTT TTCAGACCAA 
CCAGATCTGG CACAGTGGAC GAGAGCAGTA ACACAGCAGA TAAAGGTGGT CCAGATGCTG 
AGGAGAAAGC ACAATGCTGC TGACACGTCT TCTTCAGAGG ACACAGAAGC CATGAATATA 
ATACCGATCC CCGAAGGCTT CAGGCCTGAT TTTTATCCTA GGAACCCATA TTCTGATAGC 
ATAAAAGAAA TGTTAACGAC ATTTGGAACG GCTGCTTACA AGGTGGGACT GAAGGTTCAT 
CCTAATGAAG GTGACCCCCG TGTGCCCATC CTGTGCTGGG GGACCTGTGC ATACACCATC 
CAGAGCATAG AAAGAATTTT GAGTGATGAG GAGAAGCCTG TTTTTGGACC TCTGCCTTGT 
AGACTGGACG ACTGTCTCAG GTCGTTAACA CGGTTTGCAG CAGCACATTG GACAGTGGCG 
TTACTTCCTG TGGTACAAGG ACACTTCTGT AAACTCTTTG CATCCTTGGT GCCTAGTGAC 
AGCTATGAAG ACCTCCCGTG CATACTAGAC ATCGACATGT TTCACTTGCT GGTGGGCCTG 
GTGCTCGCTT TCCCAGCTCT GCAGTGTCAG GATTTTTCAG GAAGCAGCCT GGCCACTGGG 
GACCTGCACA TCTTCCACTT GGTTACCATG GCACACATCG TACAGATCTT ACTTACCTCA 
TGTACAGAAG AGAATGGCAT GGATCAAGAG AATCCCACTG GGGAAGAAGA ACTGGCCATT 
CTCTCTTTGC ACAAAACACT TCACCAGTAT ACTGGAAGTG CCTTGAAAGA AGCCCCCTCC 
GGCTGGCACC TGTGGAGGAG CGTCCGGGCC GCCATCATGC CTTTCCTCAA GTGCTCTGCT 
TTGTTTTTCC ACTATTTAAA TGGAGTCCCG GCCCCTCCAG ACCTTCAAGT TTCTGGAACA 
AGCCATTTTG AACACTTATG TAACTACCTT TCCCTACCAA CCAACCTCAT TCACCTTTTT 
CAAGAAAACA GTGACATCAT GAACTCCCTG ATTGAAAGTT GGTGCCAGAA CAGTGAAGTT 
AAACGGTATC TAAATGGCGA GAGAGGAGCG ATAAGCTACC CCAGAGGAGC TAACAAACTG 
ATAGACCTTC CAGAGGATTA CAGCAGCCTC ATTAACCAAG CATCCAATTT CTCGTGCCCC 
AAATCAGGTG GCGACAAGAG CAGAGCTCCT ACTCTGTGCC TCGTGTGTGG GAGTCTCCTC 
TGCTCTCAGA GTTACTGCTG CCAAGCTGAG CTGGAGGGTG AGGACGTCGG AGCCTGCACA 
GCACACACCT ACTCCTGCGG CTCCGGGGCC GGCATCTTCC TGAGAGTGCG GGAATGTCAG 
GTGCTATTTT TAGCTGGCAA AACCAAAGGA TGTTTTTATT CTCCTCCTTA CCTTGACGAC 
TATGGAGAGA CCGACCAGGG ACTCAGACGA GGAAATCCTT TACATTTATG CCAAGAGCGG 
TTTCGAAAGA TCCAGAAGCT CTGGCAGCAG CATAGTATCA CAGAGGAGAT CGGACACGCG 
CAGGAGGCTA ACCAGACCCT GGTCGGAATT GACTGGCAGC ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001170845.1
CDS	314..5581
Translation	MASEMEPEVQAIDRSLLECSAEEIAGRWLQATDLNREVYQHLAHCVPKIYCRGPNPFPQKEDTLAQHILLGPMEWYICAEDPALGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHKLSSSEVVEEEDPLVHLSEDVIARTYNIFAIMFRYAVDILTWEKESELPEDLEVAEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCDQAKTVIVRNTSRQTKPLKVQVMHSSVAAHQNFGLKALSWLGSVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFALRFAKNYERLQRDYVTDDHDREFSVADLSVQIFTVPSLARMLLTEENLMTVIIKAFMDHLKHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDELRQKFLQGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMVQDWCALDEKVLIEAYKKCLAVLTQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPISRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQLFSTPDYGKRFSSEVTHKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVAATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIESVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRENKEDTALPPPALPPFCPLFASLVNILQCDVMLYIMGTILQWAVEHHGSAWSESMLQRVLHLIGMALQEEKHHLENAVEGHVQTFTFTQKISKPGDAPHNSPSILAMLETLQNAPSLEAHKDMIRWLLKMFNAIKKIRECSSSSPVAEAEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDTSASATLDSSPPVSDAALTALGPAQTQVPEPRQFVTCILCQEEQEVTVGSRAMVLAAFVQRSTVLSKDRTKTIADPEKYDPLFMHPDLSCGTHTGSCGHVMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRSILSRRLNFSDQPDLAQWTRAVTQQIKVVQMLRRKHNAADTSSSEDTEAMNIIPIPEGFRPDFYPRNPYSDSIKEMLTTFGTAAYKVGLKVHPNEGDPRVPILCWGTCAYTIQSIERILSDEEKPVFGPLPCRLDDCLRSLTRFAAAHWTVALLPVVQGHFCKLFASLVPSDSYEDLPCILDIDMFHLLVGLVLAFPALQCQDFSGSSLATGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPTGEEELAILSLHKTLHQYTGSALKEAPSGWHLWRSVRAAIMPFLKCSALFFHYLNGVPAPPDLQVSGTSHFEHLCNYLSLPTNLIHLFQENSDIMNSLIESWCQNSEVKRYLNGERGAISYPRGANKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGSLLCSQSYCCQAELEGEDVGACTAHTYSCGSGAGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCQERFRKIQKLWQQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	NM_001177374.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177374.1

ORF Insert Sequence:

ATGGCGTCGG AGATGGAGCC CGAGGTGCAG GCCATCGACC GCAGTTTGCT GGAATGTTCT 
GCCGAAGAGA TCGCAGGGAG ATGGCTGCAA GCAACCGACC TCAACAGAGA AGTGTACCAG 
CATTTAGCCC ACTGTGTGCC CAAAATCTAC TGCCGGGGCC CTAACCCCTT CCCTCAGAAG 
GAAGACACGC TGGCACAGCA CATCCTGCTG GGACCGATGG AGTGGTACAT CTGCGCTGAA 
GACCCTGCGC TGGGATTTCC AAAGCTCGAG CAGGCAAACA AGCCTTCTCA CCTCTGTGGC 
CGAGTGTTTA AAGTGGGGGA ACCTACATAC TCCTGCAGAG ACTGTGCAGT TGACCCCACC 
TGTGTTTTAT GCATGGAGTG CTTCCTGGGA AGTATCCATA GAGACCATCG ATATAGGATG 
ACCACATCGG GAGGAGGGGG CTTCTGTGAC TGTGGTGACA CTGAGGCGTG GAAAGAGGGA 
CCTTACTGCC AGAAGCACAA GCTCAGCAGC TCTGAAGTTG TGGAGGAGGA GGATCCTCTT 
GTGCATCTAT CAGAAGATGT GATCGCCAGA ACTTACAACA TTTTTGCTAT TATGTTTCGA 
TATGCAGTAG ATATACTGAC CTGGGAAAAA GAAAGTGAAT TGCCTGAAGA CTTAGAAGTG 
GCAGAGAAGA GTGACACCTA CTACTGCATG CTGTTTAATG ATGAGGTTCA CACCTATGAG 
CAAGTCATTT ATACCCTTCA GAAAGCTGTG AACTGTACAC AGAAGGAAGC CATTGGCTTT 
GCAACTACAG TTGATCGAGA TGGCCGTAGG TCTGTCCGAT ATGGAGATTT CCAGTACTGT 
GATCAAGCAA AGACAGTCAT TGTGAGGAAC ACCAGCAGAC AGACCAAGCC GCTCAAAGTT 
CAAGTTATGC ACTCCTCCGT GGCTGCTCAT CAGAATTTTG GTTTGAAAGC TCTGTCGTGG 
CTGGGAAGTG TTATTGGATA CTCAGATGGC CTTCGCAGGA TTTTGTGTCA AGTTGGATTA 
CAAGAAGGTC CAGATGGCGA AAACTCTTCT CTGGTCGACA GACTGATGCT TAATGATTCC 
AAATTATGGA AAGGGGCTAG GAGTGTGTAT CACCAGTTGT TCATGAGCAG CCTGCTCATG 
GACCTCAAGT ATAAGAAGCT GTTCGCGCTT CGATTTGCTA AAAACTATGA GCGTTTGCAG 
AGAGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAG TCGCAGACCT CTCGGTTCAG 
ATATTCACAG TTCCTTCACT TGCGCGAATG CTCCTCACAG AAGAGAACCT GATGACCGTT 
ATCATTAAGG CTTTCATGGA CCATTTGAAA CACAGAGATG CCCAGGGCAG ATTCCAGTTT 
GAACGCTACA CTGCCCTCCA AGCCTTCAAG TTCAGGAGAG TCCAGAGCCT CATCTTAGAT 
CTCAAGTATG TATTGATTAG CAAACCAACG GAGTGGTCAG ATGAGCTGAG GCAGAAGTTC 
TTACAAGGGT TCGATGCCTT CTTGGAATTA CTGAAGTGCA TGCAGGGAAT GGACCCGATC 
ACGCGTCAGG TGGGACAGCA CATTGAGATG GAGCCAGAGT GGGAAGCAGC CTTCACACTG 
CAGATGAAGC TGACACACGT CATCTCAATG GTGCAGGACT GGTGTGCTCT GGACGAAAAA 
GTGTTAATTG AAGCTTACAA GAAATGCCTG GCTGTGCTGA CACAGTGTCA TGGCGGATTT 
ACTGATGGTG AACAGCCAAT CACACTCAGT ATTTGTGGAC ACTCGGTGGA AACCATCAGA 
TACTGTGTTT CCCAAGAAAA AGTTAGCATT CACCTCCCAA TTTCTCGCTT GCTTGCAGGT 
TTGCATGTAT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA 
AGTGAACTGA GCCCACCCAT GTTGATAGAA CATCCTCTTA GATGTCTTGT CTTATGTGCT 
CAAGTGCATG CTGGGATGTG GAGAAGAAAT GGCTTCTCTC TAGTAAATCA GATCTATTAC 
TACCATAATG TGAAATGCAG GCGAGAGATG TTCGACAAGG ACATAGTGAT GCTTCAGACA 
GGTGTCTCCA TGATGGACCC AAACCACTTC CTGATGATCA TGCTCAGCCG CTTTGAACTC 
TATCAGCTCT TCAGCACGCC TGACTATGGG AAGAGATTCA GTTCTGAGGT TACCCATAAG 
GACGTCGTTC AGCAGAACAA CACTCTGATC GAAGAGATGC TCTACCTCAT CATCATGCTT 
GTGGGAGAAA GATTCAACCC TGGGGTTGGA CAGGTGGCTG CCACAGATGA AATCAAGAGG 
GAGATTATCC ATCAGTTGAG CATCAAGCCT ATGGCTCACA GTGAGCTGGT GAAGTCTCTG 
CCTGAAGATG AGAACAAGGA GACCGGCATG GAGAGCGTCA TCGAGTCCGT TGCACATTTC 
AAGAAACCTG GGCTCACAGG GCGAGGCATG TATGAGCTGA AGCCAGAGTG TGCCAAAGAG 
TTCAACCTGT ATTTTTATCA TTTCTCCAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAG 
CGGAAATTGA AAAGAGAAAA TAAAGAAGAT ACAGCACTCC CTCCTCCGGC TTTGCCACCG 
TTCTGCCCTT TGTTCGCGAG TCTGGTTAAC ATCTTGCAGT GTGACGTCAT GCTGTACATC 
ATGGGAACGA TCCTGCAGTG GGCTGTAGAG CATCACGGGT CTGCCTGGTC AGAGTCCATG 
CTACAGAGGG TGCTGCATTT GATCGGGATG GCTCTCCAGG AAGAGAAGCA CCACTTGGAG 
AACGCCGTGG AAGGGCACGT GCAGACCTTC ACCTTCACAC AGAAGATTTC AAAGCCTGGT 
GATGCACCAC ATAACTCCCC GAGCATCCTA GCTATGCTGG AGACCTTGCA GAACGCCCCC 
TCCCTGGAAG CCCACAAGGA CATGATCAGG TGGTTGCTAA AGATGTTTAA TGCAATTAAG 
AAGATAAGAG AGTGTTCATC CAGCAGCCCT GTGGCCGAGG CGGAGGGAAC CATAATGGAG 
GAGAGCTCAA GAGACAAGGA CAAAGCAGAG AGGAAAAGAA AAGCCGAGAT CGCCAGACTG 
CGCCGGGAGA AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTCAT TGACGAAAAC 
AAAGAGCTCT TCCAGCAGAC CCTAGAGCTG GACACCTCTG CCTCTGCCAC TCTTGACAGC 
AGCCCTCCCG TTTCAGACGC AGCTCTTACA GCACTGGGCC CAGCACAGAC ACAGGTCCCT 
GAACCGAGAC AGTTTGTCAC CTGTATATTA TGTCAAGAGG AGCAAGAGGT GACTGTGGGA 
AGCAGGGCGA TGGTCTTGGC AGCGTTTGTT CAGAGGTCAA CGGTTCTGTC AAAAGACAGG 
ACGAAAACCA TCGCGGACCC AGAAAAATAT GATCCATTAT TCATGCACCC CGATCTGTCT 
TGTGGGACAC ACACTGGCAG CTGTGGGCAC GTTATGCATG CCCATTGTTG GCAAAGGTAT 
TTTGATTCCG TTCAAGCCAA GGAGCAGCGA AGGCAGCAGC GGCTGCGCTT GCACACTAGC 
TACGATGTAG AGAATGGCGA GTTCCTCTGC CCGCTCTGTG AGTGCCTGAG CAACACGGTG 
ATCCCCCTGC TGCTTCCTCC CAGGAGCATC CTCAGCAGGA GGTTAAATTT TTCAGACCAA 
CCAGATCTGG CACAGTGGAC GAGAGCAGTA ACACAGCAGA TAAAGGTGGT CCAGATGCTG 
AGGAGAAAGC ACAATGCTGC TGACACGTCT TCTTCAGAGG ACACAGAAGC CATGAATATA 
ATACCGATCC CCGAAGGCTT CAGGCCTGAT TTTTATCCTA GGAACCCATA TTCTGATAGC 
ATAAAAGAAA TGTTAACGAC ATTTGGAACG GCTGCTTACA AGGTGGGACT GAAGGTTCAT 
CCTAATGAAG GTGACCCCCG TGTGCCCATC CTGTGCTGGG GGACCTGTGC ATACACCATC 
CAGAGCATAG AAAGAATTTT GAGTGATGAG GAGAAGCCTG TTTTTGGACC TCTGCCTTGT 
AGACTGGACG ACTGTCTCAG GTCGTTAACA CGGTTTGCAG CAGCACATTG GACAGTGGCG 
TTACTTCCTG TGGTACAAGG ACACTTCTGT AAACTCTTTG CATCCTTGGT GCCTAGTGAC 
AGCTATGAAG ACCTCCCGTG CATACTAGAC ATCGACATGT TTCACTTGCT GGTGGGCCTG 
GTGCTCGCTT TCCCAGCTCT GCAGTGTCAG GATTTTTCAG GAAGCAGCCT GGCCACTGGG 
GACCTGCACA TCTTCCACTT GGTTACCATG GCACACATCG TACAGATCTT ACTTACCTCA 
TGTACAGAAG AGAATGGCAT GGATCAAGAG AATCCCACTG GGGAAGAAGA ACTGGCCATT 
CTCTCTTTGC ACAAAACACT TCACCAGTAT ACTGGAAGTG CCTTGAAAGA AGCCCCCTCC 
GGCTGGCACC TGTGGAGGAG CGTCCGGGCC GCCATCATGC CTTTCCTCAA GTGCTCTGCT 
TTGTTTTTCC ACTATTTAAA TGGAGTCCCG GCCCCTCCAG ACCTTCAAGT TTCTGGAACA 
AGCCATTTTG AACACTTATG TAACTACCTT TCCCTACCAA CCAACCTCAT TCACCTTTTT 
CAAGAAAACA GTGACATCAT GAACTCCCTG ATTGAAAGTT GGTGCCAGAA CAGTGAAGTT 
AAACGGTATC TAAATGGCGA GAGAGGAGCG ATAAGCTACC CCAGAGGAGC TAACAAACTG 
ATAGACCTTC CAGAGGATTA CAGCAGCCTC ATTAACCAAG CATCCAATTT CTCGTGCCCC 
AAATCAGGTG GCGACAAGAG CAGAGCTCCT ACTCTGTGCC TCGTGTGTGG GAGTCTCCTC 
TGCTCTCAGA GTTACTGCTG CCAAGCTGAG CTGGAGGGTG AGGACGTCGG AGCCTGCACA 
GCACACACCT ACTCCTGCGG CTCCGGGGCC GGCATCTTCC TGAGAGTGCG GGAATGTCAG 
GTGCTATTTT TAGCTGGCAA AACCAAAGGA TGTTTTTATT CTCCTCCTTA CCTTGACGAC 
TATGGAGAGA CCGACCAGGG ACTCAGACGA GGAAATCCTT TACATTTATG CCAAGAGCGG 
TTTCGAAAGA TCCAGAAGCT CTGGCAGCAG CATAGTATCA CAGAGGAGAT CGGACACGCG 
CAGGAGGCTA ACCAGACCCT GGTCGGAATT GACTGGCAGC ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

UBR2 Enriched in p53 Deficient Mouse Bone Marrow Mesenchymal Stem Cell-Exosome Promoted Gastric Cancer Progression via Wnt/?-Catenin Pathway.
Stem cells (Dayton, Ohio)35(11)2267-2279(2017 Nov)
Mao J,Liang Z,Zhang B,Yang H,Li X,Fu H,Zhang X,Yan Y,Xu W,Qian H

Mobilization of LINE-1 retrotransposons is restricted by
eLife6(2017 Aug)
MacLennan M,Garc?a-Ca?adas M,Reichmann J,Khazina E,Wagner G,Playfoot CJ,Salvador-Palomeque C,Mann AR,Peressini P,Sanchez L,Dobie K,Read D,Hung CC,Eskeland R,Meehan RR,Weichenrieder O,Garc?a-P?rez JL,Adams IR

Tex19.1 promotes Spo11-dependent meiotic recombination in mouse spermatocytes.
PLoS genetics13(7)e1006904(2017 Jul)
Crichton JH,Playfoot CJ,MacLennan M,Read D,Cooke HJ,Adams IR

Signaling mechanism of tumor cell-induced up-regulation of E3 ubiquitin ligase UBR2.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology27(7)2893-901(2013 Jul)
Zhang G,Lin RK,Kwon YT,Li YP

The N-end rule pathway counteracts cell death by destroying proapoptotic protein fragments.
Proceedings of the National Academy of Sciences of the United States of America109(27)E1839-47(2012 Jul)
Piatkov KI,Brower CS,Varshavsky A

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Ubr2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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