Wt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wt1
Entrez Gene ID	22431
Full Name	Wilms tumor 1 homolog
Synonyms	D630046I19Rik,Wt-1
General protein information	Preferred Names Wilms tumor 1 homolog Names Wilms tumor protein homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 E2-E3\|2 55.06 cM
Summary	This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system, and the orthologous human gene is mutated in a small subset of patients with Wilm's tumors. Alternative splicing has been noted for this gene, however, the full-length nature of these variants is not known. The mRNA for this gene has been shown to initiate translation from non-AUG (CUG) and AUG translation start sites, resulting in different isoforms. [provided by RefSeq, Apr 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_144783.2	NP_659032.3	Wilms tumor protein homolog

Pathways from BioSystems

Homologies

Bos taurus (cattle)	WT1	XP_003587050.2
Homo sapiens (human)	WT1	NP_077742.2
Mus musculus (house mouse)	Wt1	NP_659032.3
Gallus gallus (chicken)	WT1	NP_990547.1
Danio rerio (zebrafish)	wt1a	NP_571121.1
	WT1	XP_001084704.2
Rattus norvegicus (Norway rat)	Wt1	NP_113722.2
Xenopus tropicalis (tropical clawed frog)	wt1	NP_001135625.1
Pan troglodytes (chimpanzee)	WT1	XP_001138640.3
Canis lupus familiaris (dog)	WT1	XP_005631206.1

Gene Ontology
Bibliography

Related articles in PubMed

A non-AUG translational initiation event generates novel WT1 isoforms.
Bruening W, Pelletier J
The Journal of biological chemistry271(15)8646-54(1996 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

both the local oxygen environment and WT1, which enhances KDR expression, contribute to sex-specific Sox9 expression in developing murine gonads
Title: Wilms tumor protein-dependent transcription of VEGF receptor 2 and hypoxia regulate expression of the testis-promoting gene

WT1 stimulates IGFBP5 transcription in developing murine kidney.
Title: The Wilms tumor protein WT1 stimulates transcription of the gene encoding insulin-like growth factor binding protein 5 (IGFBP5).

Study reveals a novel role for Wt1 in early mammalian development and identifies proteases as critical mediators of the maternal-embryonic interaction. Data also suggest that the role of Wt1 in regulating fertility is conserved in mammals.
Title: The Wilms tumor protein Wt1 contributes to female fertility by regulating oviductal proteostasis.

WT1 regulates reporter gene expression through interaction with 3' UTR-binding sites
Title: Transcription factor Wilms' tumor 1 regulates developmental RNAs through 3' UTR interaction.

study provides novel insights into the role of WT1 and GATA4 during the sex differentiation phase and represents an approach that can be applied to assess other proteins with as yet unknown functions during gonadal development
Title: Ex vivo cultures combined with vivo-morpholino induced gene knockdown provide a system to assess the role of WT1 and GATA4 during gonad differentiation.

The following Wt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20161	NM_144783.2 Latest version!	Mus musculus Wilms tumor 1 homolog (Wt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$300.30 $429.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wt1 ( NM_144783.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_659032.3 Mus musculus Wilms tumor 1 homolog (Wt1), mRNA.

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CloneID	OMu20161
Clone ID Related Accession (Same CDS sequence)	NM_144783.2
Accession Version	NM_144783.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Mus musculus(house mouse)
Product	Wilms tumor protein homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL512584.13, DQ537939.1 and BG087452.2. On Dec 30, 2006 this sequence version replaced NM_144783.1. Sequence Note: A non-AUG (CUG) translation initiation codon is selected for this RefSeq based on experimental evidence provided in PMID:8621495, and for consistency with the human homolog. Alternative shorter isoforms, which are derived from downstream CUG and AUG initiation codons, are also described in PMID:8621495. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M55512.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 8621495 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGGACTTCC TCCTGTCGCA GGAGCCGGCT TCCACGTGTG TCCCGGAGCC GGCGTCTCAG 
CACACGCTCC GCCGGGAGCC CGGGTGCGTC CAGCAGCCGG AGCAACCTGG GGACCGAGGC 
CCCCGGAGCG CCTGGGCCAA GTCCAGCGCC GAGAATCCGC AGGATCGCAG GAGCGGAGAA 
CCGTCCGCAT CCGAGCCGCA CCTCATGGGT TCCGACGTGC GGGACCTGAA CGCGCTGCTG 
CCCGCTGTGT CTTCGCTGGG CGGCGGCGGC GGCGGCTGCG GGCTCCCTGT GAGCGGCGCA 
GCGCAGTGGG CGCCCGTGTT GGACTTCGCG CCTCCGGGCG CCTCGGCTTA CGGGTCGCTG 
GGCGGTCCCG CGCCTCCTCC CGCTCCGCCG CCGCCTCCGC CGCCACCCCA CTCCTTCATC 
AAACAGGAGC CCAGCTGGGG CGGCGCCGAG CCACACGAGG AGCAGTGCCT GAGCGCCTTC 
ACCTTGCACT TCTCGGGCCA GTTCACCGGT ACAGCCGGGG CCTGTCGCTA CGGACCCTTC 
GGTCCTCCCC CGCCCAGCCA GGCGTCCTCG GGCCAGGCCA GGATGTTCCC CAATGCGCCC 
TACCTGCCCA GCTGCCTGGA GAGCCAGCCT ACCATCCGCA ACCAAGGATA CAGCACGGTC 
ACTTTCGACG GGGCGCCCAG CTATGGCCAC ACGCCCTCGC ATCACGCGGC GCAGTTCCCC 
AACCATTCCT TCAAACACGA GGACCCCATG GGCCAGCAGG GCTCGCTGGG CGAGCAGCAG 
TACTCCGTGC CACCTCCGGT GTATGGCTGC CACACCCCTA CTGACAGTTG CACAGGCAGC 
CAGGCCCTGC TCCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACCTCCCAG 
CTTGAATGCA TGACCTGGAA TCAGATGAAC CTAGGAGCTA CCTTAAAGGG AATGGCTGCT 
GGGAGCTCCA GCTCAGTGAA ATGGACAGAA GGGCAGAGCA ACCACGGCAC AGGGTATGAG 
AGTGAGAACC ACACGGCCCC CATCCTCTGT GGTGCCCAGT ACAGAATACA CACCCACGGG 
GTCTTCCGAG GCATTCAGGA TGTGCGGCGT GTATCTGGAG TGGCCCCAAC TCTTGTCCGG 
TCAGCATCTG AAACCAGTGA GAAACGTCCT TTCATGTGTG CATACCCAGG CTGCAATAAG 
AGATATTTTA AGCTGTCCCA CTTACAGATG CATAGCCGGA AGCACACTGG TGAGAAACCA 
TACCAGTGTG ACTTCAAGGA CTGCGAGAGA AGGTTTTCTC GCTCAGACCA GCTCAAAAGA 
CACCAAAGGA GACACACAGG TGTGAAACCA TTCCAGTGTA AAACTTGTCA GCGAAAGTTT 
TCCCGGTCCG ACCATCTGAA GACCCACACC AGGACTCATA CAGGTAAAAC AAGTGAAAAG 
CCCTTCAGCT GTCGGTGGCA CAGTTGTCAG AAAAAGTTTG CGCGCTCAGA CGAATTGGTC 
CGCCATCACA ACATGCATCA GAGAAACATG ACCAAACTCC AGCTGGCGCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659032.3
CDS	269..1822
Translation	LDFLLSQEPASTCVPEPASQHTLRREPGCVQQPEQPGDRGPRSAWAKSSAENPQDRRSGEPSASEPHLMGSDVRDLNALLPAVSSLGGGGGGCGLPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTLHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPTIRNQGYSTVTFDGAPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGMAAGSSSSVKWTEGQSNHGTGYESENHTAPILCGAQYRIHTHGVFRGIQDVRRVSGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWHSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_144783.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Wilms tumor 1 homolog (Wt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144783.2

ORF Insert Sequence:

CTGGACTTCC TCCTGTCGCA GGAGCCGGCT TCCACGTGTG TCCCGGAGCC GGCGTCTCAG 
CACACGCTCC GCCGGGAGCC CGGGTGCGTC CAGCAGCCGG AGCAACCTGG GGACCGAGGC 
CCCCGGAGCG CCTGGGCCAA GTCCAGCGCC GAGAATCCGC AGGATCGCAG GAGCGGAGAA 
CCGTCCGCAT CCGAGCCGCA CCTCATGGGT TCCGACGTGC GGGACCTGAA CGCGCTGCTG 
CCCGCTGTGT CTTCGCTGGG CGGCGGCGGC GGCGGCTGCG GGCTCCCTGT GAGCGGCGCA 
GCGCAGTGGG CGCCCGTGTT GGACTTCGCG CCTCCGGGCG CCTCGGCTTA CGGGTCGCTG 
GGCGGTCCCG CGCCTCCTCC CGCTCCGCCG CCGCCTCCGC CGCCACCCCA CTCCTTCATC 
AAACAGGAGC CCAGCTGGGG CGGCGCCGAG CCACACGAGG AGCAGTGCCT GAGCGCCTTC 
ACCTTGCACT TCTCGGGCCA GTTCACCGGT ACAGCCGGGG CCTGTCGCTA CGGACCCTTC 
GGTCCTCCCC CGCCCAGCCA GGCGTCCTCG GGCCAGGCCA GGATGTTCCC CAATGCGCCC 
TACCTGCCCA GCTGCCTGGA GAGCCAGCCT ACCATCCGCA ACCAAGGATA CAGCACGGTC 
ACTTTCGACG GGGCGCCCAG CTATGGCCAC ACGCCCTCGC ATCACGCGGC GCAGTTCCCC 
AACCATTCCT TCAAACACGA GGACCCCATG GGCCAGCAGG GCTCGCTGGG CGAGCAGCAG 
TACTCCGTGC CACCTCCGGT GTATGGCTGC CACACCCCTA CTGACAGTTG CACAGGCAGC 
CAGGCCCTGC TCCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACCTCCCAG 
CTTGAATGCA TGACCTGGAA TCAGATGAAC CTAGGAGCTA CCTTAAAGGG AATGGCTGCT 
GGGAGCTCCA GCTCAGTGAA ATGGACAGAA GGGCAGAGCA ACCACGGCAC AGGGTATGAG 
AGTGAGAACC ACACGGCCCC CATCCTCTGT GGTGCCCAGT ACAGAATACA CACCCACGGG 
GTCTTCCGAG GCATTCAGGA TGTGCGGCGT GTATCTGGAG TGGCCCCAAC TCTTGTCCGG 
TCAGCATCTG AAACCAGTGA GAAACGTCCT TTCATGTGTG CATACCCAGG CTGCAATAAG 
AGATATTTTA AGCTGTCCCA CTTACAGATG CATAGCCGGA AGCACACTGG TGAGAAACCA 
TACCAGTGTG ACTTCAAGGA CTGCGAGAGA AGGTTTTCTC GCTCAGACCA GCTCAAAAGA 
CACCAAAGGA GACACACAGG TGTGAAACCA TTCCAGTGTA AAACTTGTCA GCGAAAGTTT 
TCCCGGTCCG ACCATCTGAA GACCCACACC AGGACTCATA CAGGTAAAAC AAGTGAAAAG 
CCCTTCAGCT GTCGGTGGCA CAGTTGTCAG AAAAAGTTTG CGCGCTCAGA CGAATTGGTC 
CGCCATCACA ACATGCATCA GAGAAACATG ACCAAACTCC AGCTGGCGCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A non-AUG translational initiation event generates novel WT1 isoforms.
The Journal of biological chemistry271(15)8646-54(1996 Apr)
Bruening W,Pelletier J

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Wt1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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