Pi4ka cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pi4ka
Entrez Gene ID	224020
Full Name	phosphatidylinositol 4-kinase alpha
Synonyms	Pik4ca
General protein information	Preferred Names phosphatidylinositol 4-kinase alpha Names phosphatidylinositol 4-kinase alpha PI4-kinase alpha PI4K-alpha phosphatidylinositol 4-kinase, catalytic, alpha polypeptide ptdIns-4-kinase alpha
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006522036.3	XP_006522099.1	phosphatidylinositol 4-kinase alpha isoform X2
XM_006522037.2	XP_006522100.1	phosphatidylinositol 4-kinase alpha isoform X4
XM_006522036.4	XP_006522099.1	phosphatidylinositol 4-kinase alpha isoform X3
NM_001373955.1	NP_001360884.1	phosphatidylinositol 4-kinase alpha isoform 1
XM_030249069.1	XP_030104929.1	phosphatidylinositol 4-kinase alpha isoform X2
XM_006522034.1	XP_006522097.1	phosphatidylinositol 4-kinase alpha isoform X1
NM_001001983.2	NP_001001983.2	phosphatidylinositol 4-kinase alpha isoform 2

Pathways from BioSystems

Homologies

	PI4KA	XP_001086542.2
Gallus gallus (chicken)	PI4KA	XP_004945537.1
Bos taurus (cattle)	PI4KA	NP_777002.1
Drosophila melanogaster (fruit fly)	PI4KIIIalpha	NP_570014.3
Caenorhabditis elegans (roundworm)	CELE_Y75B8A.24	NP_499596.2
Oryza sativa (rice)	Os03g0711200	NP_001051054.1
Xenopus tropicalis (tropical clawed frog)	pi4ka	NP_001238804.1
Homo sapiens (human)	PI4KA	NP_477352.3
Pan troglodytes (chimpanzee)	PI4KA	XP_525639.3
Mus musculus (house mouse)	Pi4ka	NP_001001983.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010801	XP_318507.4
Canis lupus familiaris (dog)	PI4KA	XP_543569.5
Rattus norvegicus (Norway rat)	Pi4ka	NP_071637.1
Danio rerio (zebrafish)	pi4kaa	NP_001030144.1
Arabidopsis thaliana (thale cress)	ATPI4K ALPHA	NP_175359.2

Gene Ontology
Bibliography

Related articles in PubMed

Widespread and dynamic translational control of red blood cell development.
Alvarez-Dominguez JR, Zhang X, Hu W
Blood129(5)619-629(2017 02)

Mouse and human CRKL is dosage sensitive for cardiac outflow tract formation.
Racedo SE, McDonald-McGinn DM, Chung JH, Goldmuntz E, Zackai E, Emanuel BS, Zhou B, Funke B, Morrow BE
American journal of human genetics96(2)235-44(2015 Feb)

Selective VPS34 inhibitor blocks autophagy and uncovers a role for NCOA4 in ferritin degradation and iron homeostasis in vivo.
Dowdle WE, Nyfeler B, Nagel J, Elling RA, Liu S, Triantafellow E, Menon S, Wang Z, Honda A, Pardee G, Cantwell J, Luu C, Cornella-Taracido I, Harrington E, Fekkes P, Lei H, Fang Q, Digan ME, Burdick D, Powers AF, Helliwell SB, D'Aquin S, Bastien J, Wang H, Wiederschain D, Kuerth J, Bergman P, Schwalb D, Thomas J, Ugwonali S, Harbinski F, Tallarico J, Wilson CJ, Myer VE, Porter JA, Bussiere DE, Finan PM, Labow MA, Mao X, Hamann LG, Manning BD, Valdez RA, Nicholson T, Schirle M, Knapp MS, Keaney EP, Murphy LO
Nature cell biology16(11)1069-79(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PI4KA is essential for the maintenance of plasma membrane phosphatidylinositol 4,5-bisphosphate pools but only during strong stimulation of receptors coupled to phospholipase C activation.
Title: Pharmacological and genetic targeting of the PI4KA enzyme reveals its important role in maintaining plasma membrane phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate levels.

It was shown that PI4KIIIalpha was targeted to the plasma membrane as part of an evolutionarily conserved complex containing Efr3/rolling blackout, which was found to be a palmitoylated peripheral membrane protein.
Title: PtdIns4P synthesis by PI4KIII? at the plasma membrane and its impact on plasma membrane identity.

Loss of phosphatidylinositol 4-kinase 2alpha activity causes late onset degeneration of spinal cord axons.
Title: Loss of phosphatidylinositol 4-kinase 2alpha activity causes late onset degeneration of spinal cord axons.

The following Pi4ka gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pi4ka cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu52053	XM_006522036.4 Latest version!	Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu52054	XM_006522037.2 Latest version!	Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu86915	XM_030249069.1 Latest version!	Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu52051	NM_001373955.1 Latest version!	Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu19021	NM_001001983.2 Latest version!	Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu52051	XM_006522034.1 Latest version!	Mus musculus phosphatidylinositol 4-kinase, catalytic, alpha polypeptide (Pi4ka), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
View Old Accession Versions >>
OMu52053	XM_006522036.3	Mus musculus phosphatidylinositol 4-kinase, catalytic, alpha polypeptide (Pi4ka), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pi4ka ( XM_006522036.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522099.1 Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu52053
Clone ID Related Accession (Same CDS sequence)	XM_006522036.3 , XM_006522036.4
Accession Version	XM_006522036.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-kinase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522036.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CTGGGGCCCG GGGAGGAGGC GGCGGTGGCG GCGGCGGCGG CGGCGGCGGC 
TCGGGGTCCA GCTCTGGCTC CAGCACCTCT CGAGGCTTTT ATTTCAACAC AGTACTTTCA 
CTGGCCCGCT CCCTGGCTGT GCAGAGACCA GCATCCTTGG AGAAGGTTCA AAAGCTGCTT 
TGCATGTGCC CAGTAGATTT TCATGGAATC TTCCAACTAG ATGAAAGACG GAGAGATGCA 
GTGATTGCAT TGGGCATTTT TCTCATTGAA TCTGATCTTC AGCACAAAGA TTGTGTTGTT 
CCTTATCTTC TTCGACTTCT CAGGGGTCTT CCAAAAGTGT ATTGGGTAGA AGAAAGCACA 
GCTCGGAAAG GCAGAGGTAA CCTCCCAGTT GCAGAGAGCT TCAGCTTCTG CCTGGTGACC 
TTGCTGTCTG ATGTGGCTTG CAGGGACCCC TCACTTAGGG ATGAGATTTT AGAGGCAATT 
TTGCAGGTTT TACATGTCCT TTTGGGAATG TGCCAGGCCT TGGAGATTCA AGAAAAAGAA 
TACCTTTGCA AGTATGCGAT CCCATGCCTG ATAGGAATCT CAAGATCTTT TGGACGTTAC 
AGCAATTCAG AAGAGTCTCT CCTCTCAAAG CTTTTCCCCA AAGTTTCTCC TCATTCCCTG 
AGAATCCCAG AAGAGCTAGA AGGTGTCCGA AGGCGTTCCT TTAATGACTT TCGCTCCATC 
CTGCCCAGCA ACCTGCTAAC TGTCTGTCAG GAGGGCACAC TGAAGAGGAA AACCAGCAGT 
GTGTCCAGCA TCTCTCAGGT TAGCCCAGAA CGTGGCATAC CCCCTCCCAG CTCCCCTGGA 
GGATCTGCAT TTCACTATTT TGAAGCTTCT TGCCTACCTG ATGGGACTGC CTTGGAGCCT 
GAATACTACT TCTCTACCAT CAGCTCCAGT TTTTCTATCT CTCCCCTGTT CAATGGTATT 
ACCTATAAGG AGTTCTGTAT TCCACTGGAA ATGCTTCGGG AACTCTTAAA CTTGGTGAAG 
AAGATTGTTG AGGAGCCTGT TCTCAAATCC TTAGATGCAA TTGTAGCCGG CGTGATGGAG 
GCCAATCCCA GTGCTGATCT GTACTATACA ACCTTCAGTG ATCCCCTGTA CCTGACCATG 
TTCAAGATGC TTCGAGATAC TCTGTACTAC ATGAAAGATC TGCCCACTTC CTTTGTGAAA 
GAGATCCATG ATTTTGTGTT GGAGCAGTTC AACATGAGCC AAGGGGAGCT CCAAAAGATT 
CTGCATGACG CAGATAGGAT CCACAGTGAG ATGAGCCCAC TCAAACTGCG CTGCCAAGCA 
AACGCTGCCT GTGTGGACCT CATGGTGTGG GCTGTGAAGG ATGAGCAGGG TGCAGAAAAC 
CTTTGCATCA AGCTGTCTGA AAAGCTGCAG TCCAAGACAT CCAGTAAGGT CATCATTGCC 
CATTTGCCCT TGCTGATCTG CTGCCTACAG GGTCTGGGCC GCCTGTGTGA AAGGTTCCCA 
GTGGTGGTCC ACTCAGTGAC GCCATCTTTG AGAGACTTTC TGGTAATTCC ATCCCCAGTG 
CTGGTGAAGC TCTATAAGTA CCACAGTCAG TACCACACAG TTGCTGGCAG TGATATAAAA 
ATCAGTGTCA CCAATGAGCA TTCCGAGTCA ACTCTGAATG TCTTGCCGGG CAAGAAGAAC 
CAGCCATCCA TGTATGAGCA GCTCCGAGAC ATCGCCATTG ACAACATCTG CAGGTGCCTG 
AAGGCCGGCC TCACCGTGGA CCCTGTGATC GTGGAGGCCT TCCTGGCCAG CCTCTCCAAC 
CGGCTCTACA TTTCCCAGGA GAGTGACAAG GATGCTCACT TGATTCCTGA CCATACCATC 
CGTGCCTTGG GACACATTGC AGTAGCTTTG AGGGACACTC CAAAGGTCAT GGAGCCAATT 
CTGCAGATCC TGCAGCAGAA ATTTTGCCAG CCCCCCTCAC CCCTTGATGT GCTCATCATC 
GACCAGCTGG GCTGCCTGGT GATCACTGGA AATCAATACA TTTATCAGGA AGTGTGGAAT 
CTCTTCCAGC AAATCAGTGT AAAGGCCAGT TCAGTTGTGT ATTCAGCCAC CAAAGACTAT 
AAAGACCATG GCTACAGGCA CTGCTCCCTA GCAGTGATCA ATGCCCTGGC CAACATTGCA 
GCCAACATTC AGGAGGAGCA TCTTGTGGAC GAGCTGTTGA TGAACCTGCT GGAACTGTTT 
GTGCAGCTGG GGCTGGAGGG GAAAAGAGCC AGTGAGAGGG CCAGTGAAAA GGGGCCTGCC 
CTGAAGGCTT CCAGCAGTGC AGGAAATTTG GGCGTGCTCA TTCCTGTAAT AGCTGTGCTC 
ACCCGACGAT TGCCACCCAT CAAAGAAGCT AAACCTAGGC TACAGAAGCT TTTCCGAGAT 
TTCTGGCTCT ACTCTGTGCT AATGGGATTC GCTGTGGAAG GCTCAGGACT CTGGCCGGAA 
GAGTGGTATG AGGGAGTCTG TGAAATAGCT ACCAAGTCAC CTCTGCTTAC CTTTCCCAGC 
AAAGAGCCTC TGCGATCAGT CCTCCAGTAC AATTCAGCTA TGAAAAACGA TACGGTCACC 
CCTGCTGAAC TGAGTGAGCT CCGAAGTACC ATCATCAACT TGCTAGATCC TCCTCCTGAG 
GTGTCTGCCC TCATCAACAA GCTGGACTTT GCCATGTCTA CCTATCTCTT GTCTGTGTAT 
CGGCTGGAGT ACATGAGGGT ACTGCGTTCT ACAGATCCAG ATCGCTTCCA GGTAATGTTC 
TGCTACTTTG AGGATAAAGC TATTCAAAAA GACAAATCTG GGATGATGCA GTGTGTCATT 
GCTGTTGCTG ACAAAGTGTT TGATGCTTTC CTGAACATGA TGGCAGAAAA AGCTAAGACC 
AAGGAGAATG AAGAGGAGCT GGAGCGGCAT GCACAGTTTC TGCTGGTGAA CTTCAACCAC 
ATTCATAAGA GGATAAGGAG AGTAGCTGAC AAATACCTAT CTGGTCTGGT GGATAAGTTT 
CCACACTTGC TCTGGAGCGG GACTGTGCTG AAGACCATGT TGGACATCCT GCAGACCCTG 
TCACTATCAC TAAGTGCTGA TATTCACAAG GACCAACCTT ACTATGACAT TCCTGATGCC 
CCCTATCGGA TCACGGTTCC TGACACATAC GAAGCCCGGG AGAGCATCGT GAAAGACTTT 
GCTGCGCGCT GTGGAATGAT CCTACAGGAA GCCATGAAGT GGGCACCCAC GGTCACTAAG 
TCCCACCTAC AGGAATATCT GAACAAGCAT CAGAACTGGG TGTCAGGACT GTCTCAGCAC 
ACAGGATTGG CCATGGCCAC TGAGAGCATC CTTCATTTTG CCGGCTACAA CAAGCAGAAC 
ACAACTCTTG GGGCAACTCA GCTAACTGAG CGTCCGGCCT GTGTGAAGAA AGACTACTCC 
AACTTCATGG CATCCTTGAA TTTGCGCAAC CGCTATGCCG GCGAGGTACA TGGAATGATT 
CGGTTCTCAG GTGCCACAGG CCAGATGTCT GACCTGAACA AAATGATGGT CCAAGATCTG 
ATCACAGCTT TGGACCACAG TCATCCTCAG CACTATACAC AAGCCATGTT CAAATTGACG 
GCAATGCTCA TTAGCAGCAA AGATTGTGAT CCACAGCTCC TTCATCATCT GTGTTGGGGT 
CCCCTCCGAA TGTTCAATGA GCATGGCATG GAGACTGCAC TAGCCTGCTG GGAGTGGCTG 
CTGGCTGGCA AGAATGGAGT GGAAGTGCCG TTTATGCGGG AGATGGCAGG AGCCTGGCAC 
ATGACAGTAG AGCAGAAGTT CGGCCTCTTT TCTGTGGAAA CAAAGGAAGC AGACCCCCTG 
GCAGCATCAG AAGCAAGTCA GCCAAGACCT TGTCCCCCCG AAGTGACACC TCACTACATC 
TGGATCGATT TCCTGGTCCA GCGGTTTGAG ATTGCCAAGT ACTGTAGCTC TGACCAGGTG 
GAGATCTTCT CAAGCCTGCT GCAGCGCTCC ATGTCCCTCA ATATTGGTGG AGCCAGGGGG 
AGCATGAACA GGCATGTGGC AGCCATTGGA CCACGTTTCA AACTGCTGAC CTTGGGGCTG 
TCCCTCCTGC ATGCTGATGT GGTTCCAAAT GCAACCATTC GAAACGTGCT TCGAGAGAAG 
ATATACTCAA CAGCCTTTGA CTATTTCAGC AGCCATAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522099.1
CDS	117..4298
Translation	MAAAGARGGGGGGGGGGGGGSGSSSGSSTSRGFYFNTVLSLARSLAVQRPASLEKVQKLLCMCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDCVVPYLLRLLRGLPKVYWVEESTARKGRGNLPVAESFSFCLVTLLSDVACRDPSLRDEILEAILQVLHVLLGMCQALEIQEKEYLCKYAIPCLIGISRSFGRYSNSEESLLSKLFPKVSPHSLRIPEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPERGIPPPSSPGGSAFHYFEASCLPDGTALEPEYYFSTISSSFSISPLFNGITYKEFCIPLEMLRELLNLVKKIVEEPVLKSLDAIVAGVMEANPSADLYYTTFSDPLYLTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFNMSQGELQKILHDADRIHSEMSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHLPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGSDIKISVTNEHSESTLNVLPGKKNQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQEEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMRVLRSTDPDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQLTERPACVKKDYSNFMASLNLRNRYAGEVHGMIRFSGATGQMSDLNKMMVQDLITALDHSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWHMTVEQKFGLFSVETKEADPLAASEASQPRPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSLNIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSSHN

Target ORF information:

RefSeq Version	XM_006522036.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522036.4

ORF Insert Sequence:

ATGGCGGCTG CTGGGGCCCG GGGAGGAGGC GGCGGTGGCG GCGGCGGCGG CGGCGGCGGC 
TCGGGGTCCA GCTCTGGCTC CAGCACCTCT CGAGGCTTTT ATTTCAACAC AGTACTTTCA 
CTGGCCCGCT CCCTGGCTGT GCAGAGACCA GCATCCTTGG AGAAGGTTCA AAAGCTGCTT 
TGCATGTGCC CAGTAGATTT TCATGGAATC TTCCAACTAG ATGAAAGACG GAGAGATGCA 
GTGATTGCAT TGGGCATTTT TCTCATTGAA TCTGATCTTC AGCACAAAGA TTGTGTTGTT 
CCTTATCTTC TTCGACTTCT CAGGGGTCTT CCAAAAGTGT ATTGGGTAGA AGAAAGCACA 
GCTCGGAAAG GCAGAGGTAA CCTCCCAGTT GCAGAGAGCT TCAGCTTCTG CCTGGTGACC 
TTGCTGTCTG ATGTGGCTTG CAGGGACCCC TCACTTAGGG ATGAGATTTT AGAGGCAATT 
TTGCAGGTTT TACATGTCCT TTTGGGAATG TGCCAGGCCT TGGAGATTCA AGAAAAAGAA 
TACCTTTGCA AGTATGCGAT CCCATGCCTG ATAGGAATCT CAAGATCTTT TGGACGTTAC 
AGCAATTCAG AAGAGTCTCT CCTCTCAAAG CTTTTCCCCA AAGTTTCTCC TCATTCCCTG 
AGAATCCCAG AAGAGCTAGA AGGTGTCCGA AGGCGTTCCT TTAATGACTT TCGCTCCATC 
CTGCCCAGCA ACCTGCTAAC TGTCTGTCAG GAGGGCACAC TGAAGAGGAA AACCAGCAGT 
GTGTCCAGCA TCTCTCAGGT TAGCCCAGAA CGTGGCATAC CCCCTCCCAG CTCCCCTGGA 
GGATCTGCAT TTCACTATTT TGAAGCTTCT TGCCTACCTG ATGGGACTGC CTTGGAGCCT 
GAATACTACT TCTCTACCAT CAGCTCCAGT TTTTCTATCT CTCCCCTGTT CAATGGTATT 
ACCTATAAGG AGTTCTGTAT TCCACTGGAA ATGCTTCGGG AACTCTTAAA CTTGGTGAAG 
AAGATTGTTG AGGAGCCTGT TCTCAAATCC TTAGATGCAA TTGTAGCCGG CGTGATGGAG 
GCCAATCCCA GTGCTGATCT GTACTATACA ACCTTCAGTG ATCCCCTGTA CCTGACCATG 
TTCAAGATGC TTCGAGATAC TCTGTACTAC ATGAAAGATC TGCCCACTTC CTTTGTGAAA 
GAGATCCATG ATTTTGTGTT GGAGCAGTTC AACATGAGCC AAGGGGAGCT CCAAAAGATT 
CTGCATGACG CAGATAGGAT CCACAGTGAG ATGAGCCCAC TCAAACTGCG CTGCCAAGCA 
AACGCTGCCT GTGTGGACCT CATGGTGTGG GCTGTGAAGG ATGAGCAGGG TGCAGAAAAC 
CTTTGCATCA AGCTGTCTGA AAAGCTGCAG TCCAAGACAT CCAGTAAGGT CATCATTGCC 
CATTTGCCCT TGCTGATCTG CTGCCTACAG GGTCTGGGCC GCCTGTGTGA AAGGTTCCCA 
GTGGTGGTCC ACTCAGTGAC GCCATCTTTG AGAGACTTTC TGGTAATTCC ATCCCCAGTG 
CTGGTGAAGC TCTATAAGTA CCACAGTCAG TACCACACAG TTGCTGGCAG TGATATAAAA 
ATCAGTGTCA CCAATGAGCA TTCCGAGTCA ACTCTGAATG TCTTGCCGGG CAAGAAGAAC 
CAGCCATCCA TGTATGAGCA GCTCCGAGAC ATCGCCATTG ACAACATCTG CAGGTGCCTG 
AAGGCCGGCC TCACCGTGGA CCCTGTGATC GTGGAGGCCT TCCTGGCCAG CCTCTCCAAC 
CGGCTCTACA TTTCCCAGGA GAGTGACAAG GATGCTCACT TGATTCCTGA CCATACCATC 
CGTGCCTTGG GACACATTGC AGTAGCTTTG AGGGACACTC CAAAGGTCAT GGAGCCAATT 
CTGCAGATCC TGCAGCAGAA ATTTTGCCAG CCCCCCTCAC CCCTTGATGT GCTCATCATC 
GACCAGCTGG GCTGCCTGGT GATCACTGGA AATCAATACA TTTATCAGGA AGTGTGGAAT 
CTCTTCCAGC AAATCAGTGT AAAGGCCAGT TCAGTTGTGT ATTCAGCCAC CAAAGACTAT 
AAAGACCATG GCTACAGGCA CTGCTCCCTA GCAGTGATCA ATGCCCTGGC CAACATTGCA 
GCCAACATTC AGGAGGAGCA TCTTGTGGAC GAGCTGTTGA TGAACCTGCT GGAACTGTTT 
GTGCAGCTGG GGCTGGAGGG GAAAAGAGCC AGTGAGAGGG CCAGTGAAAA GGGGCCTGCC 
CTGAAGGCTT CCAGCAGTGC AGGAAATTTG GGCGTGCTCA TTCCTGTAAT AGCTGTGCTC 
ACCCGACGAT TGCCACCCAT CAAAGAAGCT AAACCTAGGC TACAGAAGCT TTTCCGAGAT 
TTCTGGCTCT ACTCTGTGCT AATGGGATTC GCTGTGGAAG GCTCAGGACT CTGGCCGGAA 
GAGTGGTATG AGGGAGTCTG TGAAATAGCT ACCAAGTCAC CTCTGCTTAC CTTTCCCAGC 
AAAGAGCCTC TGCGATCAGT CCTCCAGTAC AATTCAGCTA TGAAAAACGA TACGGTCACC 
CCTGCTGAAC TGAGTGAGCT CCGAAGTACC ATCATCAACT TGCTAGATCC TCCTCCTGAG 
GTGTCTGCCC TCATCAACAA GCTGGACTTT GCCATGTCTA CCTATCTCTT GTCTGTGTAT 
CGGCTGGAGT ACATGAGGGT ACTGCGTTCT ACAGATCCAG ATCGCTTCCA GGTAATGTTC 
TGCTACTTTG AGGATAAAGC TATTCAAAAA GACAAATCTG GGATGATGCA GTGTGTCATT 
GCTGTTGCTG ACAAAGTGTT TGATGCTTTC CTGAACATGA TGGCAGAAAA AGCTAAGACC 
AAGGAGAATG AAGAGGAGCT GGAGCGGCAT GCACAGTTTC TGCTGGTGAA CTTCAACCAC 
ATTCATAAGA GGATAAGGAG AGTAGCTGAC AAATACCTAT CTGGTCTGGT GGATAAGTTT 
CCACACTTGC TCTGGAGCGG GACTGTGCTG AAGACCATGT TGGACATCCT GCAGACCCTG 
TCACTATCAC TAAGTGCTGA TATTCACAAG GACCAACCTT ACTATGACAT TCCTGATGCC 
CCCTATCGGA TCACGGTTCC TGACACATAC GAAGCCCGGG AGAGCATCGT GAAAGACTTT 
GCTGCGCGCT GTGGAATGAT CCTACAGGAA GCCATGAAGT GGGCACCCAC GGTCACTAAG 
TCCCACCTAC AGGAATATCT GAACAAGCAT CAGAACTGGG TGTCAGGACT GTCTCAGCAC 
ACAGGATTGG CCATGGCCAC TGAGAGCATC CTTCATTTTG CCGGCTACAA CAAGCAGAAC 
ACAACTCTTG GGGCAACTCA GCTAACTGAG CGTCCGGCCT GTGTGAAGAA AGACTACTCC 
AACTTCATGG CATCCTTGAA TTTGCGCAAC CGCTATGCCG GCGAGGTACA TGGAATGATT 
CGGTTCTCAG GTGCCACAGG CCAGATGTCT GACCTGAACA AAATGATGGT CCAAGATCTG 
ATCACAGCTT TGGACCACAG TCATCCTCAG CACTATACAC AAGCCATGTT CAAATTGACG 
GCAATGCTCA TTAGCAGCAA AGATTGTGAT CCACAGCTCC TTCATCATCT GTGTTGGGGT 
CCCCTCCGAA TGTTCAATGA GCATGGCATG GAGACTGCAC TAGCCTGCTG GGAGTGGCTG 
CTGGCTGGCA AGAATGGAGT GGAAGTGCCG TTTATGCGGG AGATGGCAGG AGCCTGGCAC 
ATGACAGTAG AGCAGAAGTT CGGCCTCTTT TCTGTGGAAA CAAAGGAAGC AGACCCCCTG 
GCAGCATCAG AAGCAAGTCA GCCAAGACCT TGTCCCCCCG AAGTGACACC TCACTACATC 
TGGATCGATT TCCTGGTCCA GCGGTTTGAG ATTGCCAAGT ACTGTAGCTC TGACCAGGTG 
GAGATCTTCT CAAGCCTGCT GCAGCGCTCC ATGTCCCTCA ATATTGGTGG AGCCAGGGGG 
AGCATGAACA GGCATGTGGC AGCCATTGGA CCACGTTTCA AACTGCTGAC CTTGGGGCTG 
TCCCTCCTGC ATGCTGATGT GGTTCCAAAT GCAACCATTC GAAACGTGCT TCGAGAGAAG 
ATATACTCAA CAGCCTTTGA CTATTTCAGC AGCCATAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi4ka ( XM_006522037.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522100.1 Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu52054
Clone ID Related Accession (Same CDS sequence)	XM_006522037.2
Accession Version	XM_006522037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-kinase alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAGT GTGTCATTGC TGTTGCTGAC AAAGTGTTTG ATGCTTTCCT GAACATGATG 
GCAGAAAAAG CTAAGACCAA GGAGAATGAA GAGGAGCTGG AGCGGCATGC ACAGTTTCTG 
CTGGTGAACT TCAACCACAT TCATAAGAGG ATAAGGAGAG TAGCTGACAA ATACCTATCT 
GGTCTGGTGG ATAAGTTTCC ACACTTGCTC TGGAGCGGGA CTGTGCTGAA GACCATGTTG 
GACATCCTGC AGACCCTGTC ACTATCACTA AGTGCTGATA TTCACAAGGA CCAACCTTAC 
TATGACATTC CTGATGCCCC CTATCGGATC ACGGTTCCTG ACACATACGA AGCCCGGGAG 
AGCATCGTGA AAGACTTTGC TGCGCGCTGT GGAATGATCC TACAGGAAGC CATGAAGTGG 
GCACCCACGG TCACTAAGTC CCACCTACAG GAATATCTGA ACAAGCATCA GAACTGGGTG 
TCAGGACTGT CTCAGCACAC AGGATTGGCC ATGGCCACTG AGAGCATCCT TCATTTTGCC 
GGCTACAACA AGCAGAACAC AACTCTTGGG GCAACTCAGC TAACTGAGCG TCCGGCCTGT 
GTGAAGAAAG ACTACTCCAA CTTCATGGCA TCCTTGAATT TGCGCAACCG CTATGCCGGC 
GAGGTACATG GAATGATTCG GTTCTCAGGT GCCACAGGCC AGATGTCTGA CCTGAACAAA 
ATGATGGTCC AAGATCTGAT CACAGCTTTG GACCACAGTC ATCCTCAGCA CTATACACAA 
GCCATGTTCA AATTGACGGC AATGCTCATT AGCAGCAAAG ATTGTGATCC ACAGCTCCTT 
CATCATCTGT GTTGGGGTCC CCTCCGAATG TTCAATGAGC ATGGCATGGA GACTGCACTA 
GCCTGCTGGG AGTGGCTGCT GGCTGGCAAG AATGGAGTGG AAGTGCCGTT TATGCGGGAG 
ATGGCAGGAG CCTGGCACAT GACAGTAGAG CAGAAGTTCG GCCTCTTTTC TGTGGAAACA 
AAGGAAGCAG ACCCCCTGGC AGCATCAGAA GCAAGTCAGC CAAGACCTTG TCCCCCCGAA 
GTGACACCTC ACTACATCTG GATCGATTTC CTGGTCCAGC GGTTTGAGAT TGCCAAGTAC 
TGTAGCTCTG ACCAGGTGGA GATCTTCTCA AGCCTGCTGC AGCGCTCCAT GTCCCTCAAT 
ATTGGTGGAG CCAGGGGGAG CATGAACAGG CATGTGGCAG CCATTGGACC ACGTTTCAAA 
CTGCTGACCT TGGGGCTGTC CCTCCTGCAT GCTGATGTGG TTCCAAATGC AACCATTCGA 
AACGTGCTTC GAGAGAAGAT ATACTCAACA GCCTTTGACT ATTTCAGTTG TCCACCAAAG 
TTTCCTACTC AAGGAGAAAA GAGGCTTCGT GAAGATATAA GCATAATGAT TAAGTTTTGG 
ACTGCCATGT TCTCAGATAA GAAGTACCTG ACTGCCAGCC AGCTCGTTCC CCCAGATAAT 
CAGGACACCC GAAGCAACTT GGATATAACT GTGGGCTCTC GACAACAGGC TACCCAGGGC 
TGGATCAACA CTTACCCCCT CTCCAGTGGC ATGTCAACCA TCTCCAAAAA GTCAGGTATG 
TCTAAGAAAA CCAACCGGGG CTCCCAGCTG CACAAGTACT ACATGAAACG CAGGACACTG 
CTGCTGTCAC TATTGGCCAC TGAGATCGAA CGCCTCATCA CTTGGTACAA CCCACTGTCA 
GCCCCAGAGC TAGAGCTGGA CCAGGCAGGA GAGAACAGCG TTGCCAACTG GAGGTCCAAG 
TACATCAGCT TGAGTGAGAA GCAGTGGAAG GACAACGTGA ACCTTGCCTG GAGCATCTCT 
CCATACCTCG CTGTGCAGCT GCCAGCCAGG TTCAAGAATA CAGAAGCCAT TGGAAATGAA 
GTGACCCGTC TCGTTCGCTT GGATCCTGGA GCTGTTAGTG ATGTCCCTGA AGCCATCAAG 
TTCCTTGTCA CCTGGCACAC CATCGATGCT GATGCTCCAG AGCTCAGCCA TGTTTTATGC 
TGGGCACCCA CGGACCCACC TACAGGCCTC TCCTACTTCT CCAGCATGTA CCCACCACAC 
CCTCTCACTG CACAGTATGG GGTAAAAGTC CTGCGATCCT TCCCTCCGGA TGCCATTCTG 
TTCTACATCC CCCAGATTGT GCAGGCCCTC AGATATGACA AGATGGGCTA TGTGCGTGAG 
TACATTCTCT GGGCTGCAGC CAAATCCCAG CTTCTGGCAC ACCAGTTTAT TTGGAACATG 
AAAACTAACA TCTACCTTGA TGAAGAAGGG CACCAGAAAG ACCCTGACAT CGGTGACCTC 
CTGGAGCAGT TAGTAGAAGA GATCACAGGG TCTCTATCAG GCCCAGCCAA AGATTTCTAT 
CAACGGGAAT TTGATTTCTT TAACAAGATC ACAAACGTGT CAGCCATCAT CAAGCCCTAC 
CCTAAAGGCG ATGAAAGGAA GAAGGCTTGT CTGTCAGCTC TGTCTGAAGT GAAGGTGCAG 
CCTGGCTGCT ACCTGCCCAG CAACCCTGAA GCAATCGTGC TAGACATTGA CTACAAGTCA 
GGGACACCAA TGCAGAGTGC CGCAAAAGCG CCGTATCTGG CCAAGTTTAA GGTGAAGAGA 
TGCGGAGTGA GTGAACTTGA AAAAGAAGGT CTGCAGTGCC GCTCAGACGC AGAGGATGAA 
TGCTTCAGCC AAGAGGCTGA TGGCAAGAAG ATCTGCTGGC AAGCAGCCAT CTTCAAAGTG 
GGGGATGACT GCCGACAGGA CATGTTAGCC CTGCAGATCA TCGACCTCTT CAAGAACATC 
TTCCAGCTGG TTGGCCTGGA CCTCTTCGTT TTTCCTTATC GGGTAGTGGC TACTGCCCCT 
GGGTGCGGAG TGATTGAGTG CATCCCAGAC TGTACCTCCC GGGACCAGCT GGGCCGCCAG 
ACTGACTTTG GCATGTATGA CTACTTTACA CGCCAGTATG GGGATGAGTC TACCTTGGCC 
TTCCAGCAGG CACGCTACAA CTTTATCAGA AGCATGGCCG CCTATAGCCT CCTGCTGTTC 
CTGCTGCAGA TCAAGGACAG GCACAATGGC AACATCATGC TGGACAAGAA GGGGCACATC 
ATCCACATCG ACTTTGGCTT CATGTTTGAA AGCTCACCGG GTGGCAACCT TGGCTGGGAA 
CCAGACATCA AGCTGACAGA CGAGATGGTG ATGATTATGG GGGGCAAAAT GGAAGCCACA 
CCCTTCAAGT GGTTCATGGA GATGTGTGTC CGAGGTTATC TGGCTGTGCG CCCCTATATG 
GATGCAGTCG TCTCCCTGGT TACACTCATG TTGGACACAG GCCTGCCCTG TTTTCGTGGC 
CAGACAATCA AACTTTTGAA ACACAGGTTC AGCCCGAACA TGACAGAGCG GGAGGCTGCG 
AATTTTATCA TGAAGGTCAT CCAGAACTGT TTCCTCAGCA ACAGGAGCCG GACCTACGAC 
ATGATCCAGT ACTACCAGAA TGATATCCCC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522100.1
CDS	847..4362
Translation	MMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQLTERPACVKKDYSNFMASLNLRNRYAGEVHGMIRFSGATGQMSDLNKMMVQDLITALDHSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWHMTVEQKFGLFSVETKEADPLAASEASQPRPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSLNIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAAKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLEQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLQCRSDAEDECFSQEADGKKICWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQNCFLSNRSRTYDMIQYYQNDIPY

Target ORF information:

RefSeq Version	XM_006522037.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522037.2

ORF Insert Sequence:

ATGATGCAGT GTGTCATTGC TGTTGCTGAC AAAGTGTTTG ATGCTTTCCT GAACATGATG 
GCAGAAAAAG CTAAGACCAA GGAGAATGAA GAGGAGCTGG AGCGGCATGC ACAGTTTCTG 
CTGGTGAACT TCAACCACAT TCATAAGAGG ATAAGGAGAG TAGCTGACAA ATACCTATCT 
GGTCTGGTGG ATAAGTTTCC ACACTTGCTC TGGAGCGGGA CTGTGCTGAA GACCATGTTG 
GACATCCTGC AGACCCTGTC ACTATCACTA AGTGCTGATA TTCACAAGGA CCAACCTTAC 
TATGACATTC CTGATGCCCC CTATCGGATC ACGGTTCCTG ACACATACGA AGCCCGGGAG 
AGCATCGTGA AAGACTTTGC TGCGCGCTGT GGAATGATCC TACAGGAAGC CATGAAGTGG 
GCACCCACGG TCACTAAGTC CCACCTACAG GAATATCTGA ACAAGCATCA GAACTGGGTG 
TCAGGACTGT CTCAGCACAC AGGATTGGCC ATGGCCACTG AGAGCATCCT TCATTTTGCC 
GGCTACAACA AGCAGAACAC AACTCTTGGG GCAACTCAGC TAACTGAGCG TCCGGCCTGT 
GTGAAGAAAG ACTACTCCAA CTTCATGGCA TCCTTGAATT TGCGCAACCG CTATGCCGGC 
GAGGTACATG GAATGATTCG GTTCTCAGGT GCCACAGGCC AGATGTCTGA CCTGAACAAA 
ATGATGGTCC AAGATCTGAT CACAGCTTTG GACCACAGTC ATCCTCAGCA CTATACACAA 
GCCATGTTCA AATTGACGGC AATGCTCATT AGCAGCAAAG ATTGTGATCC ACAGCTCCTT 
CATCATCTGT GTTGGGGTCC CCTCCGAATG TTCAATGAGC ATGGCATGGA GACTGCACTA 
GCCTGCTGGG AGTGGCTGCT GGCTGGCAAG AATGGAGTGG AAGTGCCGTT TATGCGGGAG 
ATGGCAGGAG CCTGGCACAT GACAGTAGAG CAGAAGTTCG GCCTCTTTTC TGTGGAAACA 
AAGGAAGCAG ACCCCCTGGC AGCATCAGAA GCAAGTCAGC CAAGACCTTG TCCCCCCGAA 
GTGACACCTC ACTACATCTG GATCGATTTC CTGGTCCAGC GGTTTGAGAT TGCCAAGTAC 
TGTAGCTCTG ACCAGGTGGA GATCTTCTCA AGCCTGCTGC AGCGCTCCAT GTCCCTCAAT 
ATTGGTGGAG CCAGGGGGAG CATGAACAGG CATGTGGCAG CCATTGGACC ACGTTTCAAA 
CTGCTGACCT TGGGGCTGTC CCTCCTGCAT GCTGATGTGG TTCCAAATGC AACCATTCGA 
AACGTGCTTC GAGAGAAGAT ATACTCAACA GCCTTTGACT ATTTCAGTTG TCCACCAAAG 
TTTCCTACTC AAGGAGAAAA GAGGCTTCGT GAAGATATAA GCATAATGAT TAAGTTTTGG 
ACTGCCATGT TCTCAGATAA GAAGTACCTG ACTGCCAGCC AGCTCGTTCC CCCAGATAAT 
CAGGACACCC GAAGCAACTT GGATATAACT GTGGGCTCTC GACAACAGGC TACCCAGGGC 
TGGATCAACA CTTACCCCCT CTCCAGTGGC ATGTCAACCA TCTCCAAAAA GTCAGGTATG 
TCTAAGAAAA CCAACCGGGG CTCCCAGCTG CACAAGTACT ACATGAAACG CAGGACACTG 
CTGCTGTCAC TATTGGCCAC TGAGATCGAA CGCCTCATCA CTTGGTACAA CCCACTGTCA 
GCCCCAGAGC TAGAGCTGGA CCAGGCAGGA GAGAACAGCG TTGCCAACTG GAGGTCCAAG 
TACATCAGCT TGAGTGAGAA GCAGTGGAAG GACAACGTGA ACCTTGCCTG GAGCATCTCT 
CCATACCTCG CTGTGCAGCT GCCAGCCAGG TTCAAGAATA CAGAAGCCAT TGGAAATGAA 
GTGACCCGTC TCGTTCGCTT GGATCCTGGA GCTGTTAGTG ATGTCCCTGA AGCCATCAAG 
TTCCTTGTCA CCTGGCACAC CATCGATGCT GATGCTCCAG AGCTCAGCCA TGTTTTATGC 
TGGGCACCCA CGGACCCACC TACAGGCCTC TCCTACTTCT CCAGCATGTA CCCACCACAC 
CCTCTCACTG CACAGTATGG GGTAAAAGTC CTGCGATCCT TCCCTCCGGA TGCCATTCTG 
TTCTACATCC CCCAGATTGT GCAGGCCCTC AGATATGACA AGATGGGCTA TGTGCGTGAG 
TACATTCTCT GGGCTGCAGC CAAATCCCAG CTTCTGGCAC ACCAGTTTAT TTGGAACATG 
AAAACTAACA TCTACCTTGA TGAAGAAGGG CACCAGAAAG ACCCTGACAT CGGTGACCTC 
CTGGAGCAGT TAGTAGAAGA GATCACAGGG TCTCTATCAG GCCCAGCCAA AGATTTCTAT 
CAACGGGAAT TTGATTTCTT TAACAAGATC ACAAACGTGT CAGCCATCAT CAAGCCCTAC 
CCTAAAGGCG ATGAAAGGAA GAAGGCTTGT CTGTCAGCTC TGTCTGAAGT GAAGGTGCAG 
CCTGGCTGCT ACCTGCCCAG CAACCCTGAA GCAATCGTGC TAGACATTGA CTACAAGTCA 
GGGACACCAA TGCAGAGTGC CGCAAAAGCG CCGTATCTGG CCAAGTTTAA GGTGAAGAGA 
TGCGGAGTGA GTGAACTTGA AAAAGAAGGT CTGCAGTGCC GCTCAGACGC AGAGGATGAA 
TGCTTCAGCC AAGAGGCTGA TGGCAAGAAG ATCTGCTGGC AAGCAGCCAT CTTCAAAGTG 
GGGGATGACT GCCGACAGGA CATGTTAGCC CTGCAGATCA TCGACCTCTT CAAGAACATC 
TTCCAGCTGG TTGGCCTGGA CCTCTTCGTT TTTCCTTATC GGGTAGTGGC TACTGCCCCT 
GGGTGCGGAG TGATTGAGTG CATCCCAGAC TGTACCTCCC GGGACCAGCT GGGCCGCCAG 
ACTGACTTTG GCATGTATGA CTACTTTACA CGCCAGTATG GGGATGAGTC TACCTTGGCC 
TTCCAGCAGG CACGCTACAA CTTTATCAGA AGCATGGCCG CCTATAGCCT CCTGCTGTTC 
CTGCTGCAGA TCAAGGACAG GCACAATGGC AACATCATGC TGGACAAGAA GGGGCACATC 
ATCCACATCG ACTTTGGCTT CATGTTTGAA AGCTCACCGG GTGGCAACCT TGGCTGGGAA 
CCAGACATCA AGCTGACAGA CGAGATGGTG ATGATTATGG GGGGCAAAAT GGAAGCCACA 
CCCTTCAAGT GGTTCATGGA GATGTGTGTC CGAGGTTATC TGGCTGTGCG CCCCTATATG 
GATGCAGTCG TCTCCCTGGT TACACTCATG TTGGACACAG GCCTGCCCTG TTTTCGTGGC 
CAGACAATCA AACTTTTGAA ACACAGGTTC AGCCCGAACA TGACAGAGCG GGAGGCTGCG 
AATTTTATCA TGAAGGTCAT CCAGAACTGT TTCCTCAGCA ACAGGAGCCG GACCTACGAC 
ATGATCCAGT ACTACCAGAA TGATATCCCC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi4ka ( XM_030249069.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104929.1 Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant X2, mRNA.

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CloneID	OMu86915
Clone ID Related Accession (Same CDS sequence)	XM_030249069.1
Accession Version	XM_030249069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCAGG CCTTGGAGAT TCAAGAAAAA GAATACCTTT GCAAGTATGC GATCCCATGC 
CTGATAGGAA TCTCAAGATC TTTTGGACGT TACAGCAATT CAGAAGAGTC TCTCCTCTCA 
AAGCTTTTCC CCAAAGTTTC TCCTCATTCC CTGAGAATCC CAGAAGAGCT AGAAGGTGTC 
CGAAGGCGTT CCTTTAATGA CTTTCGCTCC ATCCTGCCCA GCAACCTGCT AACTGTCTGT 
CAGGAGGGCA CACTGAAGAG GAAAACCAGC AGTGTGTCCA GCATCTCTCA GGTTAGCCCA 
GAACGTGGCA TACCCCCTCC CAGCTCCCCT GGAGGATCTG CATTTCACTA TTTTGAAGCT 
TCTTGCCTAC CTGATGGGAC TGCCTTGGAG CCTGAATACT ACTTCTCTAC CATCAGCTCC 
AGTTTTTCTA TCTCTCCCCT GTTCAATGGT ATTACCTATA AGGAGTTCTG TATTCCACTG 
GAAATGCTTC GGGAACTCTT AAACTTGGTG AAGAAGATTG TTGAGGAGCC TGTTCTCAAA 
TCCTTAGATG CAATTGTAGC CGGCGTGATG GAGGCCAATC CCAGTGCTGA TCTGTACTAT 
ACAACCTTCA GTGATCCCCT GTACCTGACC ATGTTCAAGA TGCTTCGAGA TACTCTGTAC 
TACATGAAAG ATCTGCCCAC TTCCTTTGTG AAAGAGATCC ATGATTTTGT GTTGGAGCAG 
TTCAACATGA GCCAAGGGGA GCTCCAAAAG ATTCTGCATG ACGCAGATAG GATCCACAGT 
GAGATGAGCC CACTCAAACT GCGCTGCCAA GCAAACGCTG CCTGTGTGGA CCTCATGGTG 
TGGGCTGTGA AGGATGAGCA GGGTGCAGAA AACCTTTGCA TCAAGCTGTC TGAAAAGCTG 
CAGTCCAAGA CATCCAGTAA GGTCATCATT GCCCATTTGC CCTTGCTGAT CTGCTGCCTA 
CAGGGTCTGG GCCGCCTGTG TGAAAGGTTC CCAGTGGTGG TCCACTCAGT GACGCCATCT 
TTGAGAGACT TTCTGGTAAT TCCATCCCCA GTGCTGGTGA AGCTCTATAA GTACCACAGT 
CAGTACCACA CAGTTGCTGG CAGTGATATA AAAATCAGTG TCACCAATGA GCATTCCGAG 
TCAACTCTGA ATGTCTTGCC GGGCAAGAAG AACCAGCCAT CCATGTATGA GCAGCTCCGA 
GACATCGCCA TTGACAACAT CTGCAGGTGC CTGAAGGCCG GCCTCACCGT GGACCCTGTG 
ATCGTGGAGG CCTTCCTGGC CAGCCTCTCC AACCGGCTCT ACATTTCCCA GGAGAGTGAC 
AAGGATGCTC ACTTGATTCC TGACCATACC ATCCGTGCCT TGGGACACAT TGCAGTAGCT 
TTGAGGGACA CTCCAAAGGT CATGGAGCCA ATTCTGCAGA TCCTGCAGCA GAAATTTTGC 
CAGCCCCCCT CACCCCTTGA TGTGCTCATC ATCGACCAGC TGGGCTGCCT GGTGATCACT 
GGAAATCAAT ACATTTATCA GGAAGTGTGG AATCTCTTCC AGCAAATCAG TGTAAAGGCC 
AGTTCAGTTG TGTATTCAGC CACCAAAGAC TATAAAGACC ATGGCTACAG GCACTGCTCC 
CTAGCAGTGA TCAATGCCCT GGCCAACATT GCAGCCAACA TTCAGGAGGA GCATCTTGTG 
GACGAGCTGT TGATGAACCT GCTGGAACTG TTTGTGCAGC TGGGGCTGGA GGGGAAAAGA 
GCCAGTGAGA GGGCCAGTGA AAAGGGGCCT GCCCTGAAGG CTTCCAGCAG TGCAGGAAAT 
TTGGGCGTGC TCATTCCTGT AATAGCTGTG CTCACCCGAC GATTGCCACC CATCAAAGAA 
GCTAAACCTA GGCTACAGAA GCTTTTCCGA GATTTCTGGC TCTACTCTGT GCTAATGGGA 
TTCGCTGTGG AAGGCTCAGG ACTCTGGCCG GAAGAGTGGT ATGAGGGAGT CTGTGAAATA 
GCTACCAAGT CACCTCTGCT TACCTTTCCC AGCAAAGAGC CTCTGCGATC AGTCCTCCAG 
TACAATTCAG CTATGAAAAA CGATACGGTC ACCCCTGCTG AACTGAGTGA GCTCCGAAGT 
ACCATCATCA ACTTGCTAGA TCCTCCTCCT GAGGTGTCTG CCCTCATCAA CAAGCTGGAC 
TTTGCCATGT CTACCTATCT CTTGTCTGTG TATCGGCTGG AGTACATGAG GGTACTGCGT 
TCTACAGATC CAGATCGCTT CCAGGTAATG TTCTGCTACT TTGAGGATAA AGCTATTCAA 
AAAGACAAAT CTGGGATGAT GCAGTGTGTC ATTGCTGTTG CTGACAAAGT GTTTGATGCT 
TTCCTGAACA TGATGGCAGA AAAAGCTAAG ACCAAGGAGA ATGAAGAGGA GCTGGAGCGG 
CATGCACAGT TTCTGCTGGT GAACTTCAAC CACATTCATA AGAGGATAAG GAGAGTAGCT 
GACAAATACC TATCTGGTCT GGTGGATAAG TTTCCACACT TGCTCTGGAG CGGGACTGTG 
CTGAAGACCA TGTTGGACAT CCTGCAGACC CTGTCACTAT CACTAAGTGC TGATATTCAC 
AAGGACCAAC CTTACTATGA CATTCCTGAT GCCCCCTATC GGATCACGGT TCCTGACACA 
TACGAAGCCC GGGAGAGCAT CGTGAAAGAC TTTGCTGCGC GCTGTGGAAT GATCCTACAG 
GAAGCCATGA AGTGGGCACC CACGGTCACT AAGTCCCACC TACAGGAATA TCTGAACAAG 
CATCAGAACT GGGTGTCAGG ACTGTCTCAG CACACAGGAT TGGCCATGGC CACTGAGAGC 
ATCCTTCATT TTGCCGGCTA CAACAAGCAG AACACAACTC TTGGGGCAAC TCAGCTAACT 
GAGCGTCCGG CCTGTGTGAA GAAAGACTAC TCCAACTTCA TGGCATCCTT GAATTTGCGC 
AACCGCTATG CCGGCGAGGT ACATGGAATG ATTCGGTTCT CAGGTGCCAC AGGCCAGATG 
TCTGACCTGA ACAAAATGAT GGTCCAAGAT CTGATCACAG CTTTGGACCA CAGTCATCCT 
CAGCACTATA CACAAGCCAT GTTCAAATTG ACGGCAATGC TCATTAGCAG CAAAGATTGT 
GATCCACAGC TCCTTCATCA TCTGTGTTGG GGTCCCCTCC GAATGTTCAA TGAGCATGGC 
ATGGAGACTG CACTAGCCTG CTGGGAGTGG CTGCTGGCTG GCAAGAATGG AGTGGAAGTG 
CCGTTTATGC GGGAGATGGC AGGAGCCTGG CACATGACAG TAGAGCAGAA GTTCGGCCTC 
TTTTCTGTGG AAACAAAGGA AGCAGACCCC CTGGCAGCAT CAGAAGCAAG TCAGCCAAGA 
CCTTGTCCCC CCGAAGTGAC ACCTCACTAC ATCTGGATCG ATTTCCTGGT CCAGCGGTTT 
GAGATTGCCA AGTACTGTAG CTCTGACCAG GTGGAGATCT TCTCAAGCCT GCTGCAGCGC 
TCCATGTCCC TCAATATTGG TGGAGCCAGG GGGAGCATGA ACAGGCATGT GGCAGCCATT 
GGACCACGTT TCAAACTGCT GACCTTGGGG CTGTCCCTCC TGCATGCTGA TGTGGTTCCA 
AATGCAACCA TTCGAAACGT GCTTCGAGAG AAGATATACT CAACAGCCTT TGACTATTTC 
AGTTGTCCAC CAAAGTTTCC TACTCAAGGA GAAAAGAGGC TTCGTGAAGA TATAAGCATA 
ATGATTAAGT TTTGGACTGC CATGTTCTCA GATAAGAAGT ACCTGACTGC CAGCCAGCTC 
GTTCCCCCAG ATAATCAGGA CACCCGAAGC AACTTGGATA TAACTGTGGG CTCTCGACAA 
CAGGCTACCC AGGGCTGGAT CAACACTTAC CCCCTCTCCA GTGGCATGTC AACCATCTCC 
AAAAAGTCAG GTATGTCTAA GAAAACCAAC CGGGGCTCCC AGCTGCACAA GTACTACATG 
AAACGCAGGA CACTGCTGCT GTCACTATTG GCCACTGAGA TCGAACGCCT CATCACTTGG 
TACAACCCAC TGTCAGCCCC AGAGCTAGAG CTGGACCAGG CAGGAGAGAA CAGCGTTGCC 
AACTGGAGGT CCAAGTACAT CAGCTTGAGT GAGAAGCAGT GGAAGGACAA CGTGAACCTT 
GCCTGGAGCA TCTCTCCATA CCTCGCTGTG CAGCTGCCAG CCAGGTTCAA GAATACAGAA 
GCCATTGGAA ATGAAGTGAC CCGTCTCGTT CGCTTGGATC CTGGAGCTGT TAGTGATGTC 
CCTGAAGCCA TCAAGTTCCT TGTCACCTGG CACACCATCG ATGCTGATGC TCCAGAGCTC 
AGCCATGTTT TATGCTGGGC ACCCACGGAC CCACCTACAG GCCTCTCCTA CTTCTCCAGC 
ATGTACCCAC CACACCCTCT CACTGCACAG TATGGGGTAA AAGTCCTGCG ATCCTTCCCT 
CCGGATGCCA TTCTGTTCTA CATCCCCCAG ATTGTGCAGG CCCTCAGATA TGACAAGATG 
GGCTATGTGC GTGAGTACAT TCTCTGGGCT GCAGCCAAAT CCCAGCTTCT GGCACACCAG 
TTTATTTGGA ACATGAAAAC TAACATCTAC CTTGATGAAG AAGGGCACCA GAAAGACCCT 
GACATCGGTG ACCTCCTGGA GCAGTTAGTA GAAGAGATCA CAGGGTCTCT ATCAGGCCCA 
GCCAAAGATT TCTATCAACG GGAATTTGAT TTCTTTAACA AGATCACAAA CGTGTCAGCC 
ATCATCAAGC CCTACCCTAA AGGCGATGAA AGGAAGAAGG CTTGTCTGTC AGCTCTGTCT 
GAAGTGAAGG TGCAGCCTGG CTGCTACCTG CCCAGCAACC CTGAAGCAAT CGTGCTAGAC 
ATTGACTACA AGTCAGGGAC ACCAATGCAG AGTGCCGCAA AAGCGCCGTA TCTGGCCAAG 
TTTAAGGTGA AGAGATGCGG AGTGAGTGAA CTTGAAAAAG AAGGTCTGCA GTGCCGCTCA 
GACGCAGAGG ATGAATGCTT CAGCCAAGAG GCTGATGGCA AGAAGATCTG CTGGCAAGCA 
GCCATCTTCA AAGTGGGGGA TGACTGCCGA CAGGACATGT TAGCCCTGCA GATCATCGAC 
CTCTTCAAGA ACATCTTCCA GCTGGTTGGC CTGGACCTCT TCGTTTTTCC TTATCGGGTA 
GTGGCTACTG CCCCTGGGTG CGGAGTGATT GAGTGCATCC CAGACTGTAC CTCCCGGGAC 
CAGCTGGGCC GCCAGACTGA CTTTGGCATG TATGACTACT TTACACGCCA GTATGGGGAT 
GAGTCTACCT TGGCCTTCCA GCAGGCACGC TACAACTTTA TCAGAAGCAT GGCCGCCTAT 
AGCCTCCTGC TGTTCCTGCT GCAGATCAAG GACAGGCACA ATGGCAACAT CATGCTGGAC 
AAGAAGGGGC ACATCATCCA CATCGACTTT GGCTTCATGT TTGAAAGCTC ACCGGGTGGC 
AACCTTGGCT GGGAACCAGA CATCAAGCTG ACAGACGAGA TGGTGATGAT TATGGGGGGC 
AAAATGGAAG CCACACCCTT CAAGTGGTTC ATGGAGATGT GTGTCCGAGG TTATCTGGCT 
GTGCGCCCCT ATATGGATGC AGTCGTCTCC CTGGTTACAC TCATGTTGGA CACAGGCCTG 
CCCTGTTTTC GTGGCCAGAC AATCAAACTT TTGAAACACA GGTTCAGCCC GAACATGACA 
GAGCGGGAGG CTGCGAATTT TATCATGAAG GTCATCCAGA ACTGTTTCCT CAGCAACAGG 
AGCCGGACCT ACGACATGAT CCAGTACTAC CAGAATGATA TCCCCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104929.1
CDS	483..6293
Translation	MCQALEIQEKEYLCKYAIPCLIGISRSFGRYSNSEESLLSKLFPKVSPHSLRIPEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPERGIPPPSSPGGSAFHYFEASCLPDGTALEPEYYFSTISSSFSISPLFNGITYKEFCIPLEMLRELLNLVKKIVEEPVLKSLDAIVAGVMEANPSADLYYTTFSDPLYLTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFNMSQGELQKILHDADRIHSEMSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHLPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGSDIKISVTNEHSESTLNVLPGKKNQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQEEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMRVLRSTDPDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQLTERPACVKKDYSNFMASLNLRNRYAGEVHGMIRFSGATGQMSDLNKMMVQDLITALDHSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWHMTVEQKFGLFSVETKEADPLAASEASQPRPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSLNIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAAKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLEQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLQCRSDAEDECFSQEADGKKICWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQNCFLSNRSRTYDMIQYYQNDIPY

Target ORF information:

RefSeq Version	XM_030249069.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249069.1

ORF Insert Sequence:

ATGTGCCAGG CCTTGGAGAT TCAAGAAAAA GAATACCTTT GCAAGTATGC GATCCCATGC 
CTGATAGGAA TCTCAAGATC TTTTGGACGT TACAGCAATT CAGAAGAGTC TCTCCTCTCA 
AAGCTTTTCC CCAAAGTTTC TCCTCATTCC CTGAGAATCC CAGAAGAGCT AGAAGGTGTC 
CGAAGGCGTT CCTTTAATGA CTTTCGCTCC ATCCTGCCCA GCAACCTGCT AACTGTCTGT 
CAGGAGGGCA CACTGAAGAG GAAAACCAGC AGTGTGTCCA GCATCTCTCA GGTTAGCCCA 
GAACGTGGCA TACCCCCTCC CAGCTCCCCT GGAGGATCTG CATTTCACTA TTTTGAAGCT 
TCTTGCCTAC CTGATGGGAC TGCCTTGGAG CCTGAATACT ACTTCTCTAC CATCAGCTCC 
AGTTTTTCTA TCTCTCCCCT GTTCAATGGT ATTACCTATA AGGAGTTCTG TATTCCACTG 
GAAATGCTTC GGGAACTCTT AAACTTGGTG AAGAAGATTG TTGAGGAGCC TGTTCTCAAA 
TCCTTAGATG CAATTGTAGC CGGCGTGATG GAGGCCAATC CCAGTGCTGA TCTGTACTAT 
ACAACCTTCA GTGATCCCCT GTACCTGACC ATGTTCAAGA TGCTTCGAGA TACTCTGTAC 
TACATGAAAG ATCTGCCCAC TTCCTTTGTG AAAGAGATCC ATGATTTTGT GTTGGAGCAG 
TTCAACATGA GCCAAGGGGA GCTCCAAAAG ATTCTGCATG ACGCAGATAG GATCCACAGT 
GAGATGAGCC CACTCAAACT GCGCTGCCAA GCAAACGCTG CCTGTGTGGA CCTCATGGTG 
TGGGCTGTGA AGGATGAGCA GGGTGCAGAA AACCTTTGCA TCAAGCTGTC TGAAAAGCTG 
CAGTCCAAGA CATCCAGTAA GGTCATCATT GCCCATTTGC CCTTGCTGAT CTGCTGCCTA 
CAGGGTCTGG GCCGCCTGTG TGAAAGGTTC CCAGTGGTGG TCCACTCAGT GACGCCATCT 
TTGAGAGACT TTCTGGTAAT TCCATCCCCA GTGCTGGTGA AGCTCTATAA GTACCACAGT 
CAGTACCACA CAGTTGCTGG CAGTGATATA AAAATCAGTG TCACCAATGA GCATTCCGAG 
TCAACTCTGA ATGTCTTGCC GGGCAAGAAG AACCAGCCAT CCATGTATGA GCAGCTCCGA 
GACATCGCCA TTGACAACAT CTGCAGGTGC CTGAAGGCCG GCCTCACCGT GGACCCTGTG 
ATCGTGGAGG CCTTCCTGGC CAGCCTCTCC AACCGGCTCT ACATTTCCCA GGAGAGTGAC 
AAGGATGCTC ACTTGATTCC TGACCATACC ATCCGTGCCT TGGGACACAT TGCAGTAGCT 
TTGAGGGACA CTCCAAAGGT CATGGAGCCA ATTCTGCAGA TCCTGCAGCA GAAATTTTGC 
CAGCCCCCCT CACCCCTTGA TGTGCTCATC ATCGACCAGC TGGGCTGCCT GGTGATCACT 
GGAAATCAAT ACATTTATCA GGAAGTGTGG AATCTCTTCC AGCAAATCAG TGTAAAGGCC 
AGTTCAGTTG TGTATTCAGC CACCAAAGAC TATAAAGACC ATGGCTACAG GCACTGCTCC 
CTAGCAGTGA TCAATGCCCT GGCCAACATT GCAGCCAACA TTCAGGAGGA GCATCTTGTG 
GACGAGCTGT TGATGAACCT GCTGGAACTG TTTGTGCAGC TGGGGCTGGA GGGGAAAAGA 
GCCAGTGAGA GGGCCAGTGA AAAGGGGCCT GCCCTGAAGG CTTCCAGCAG TGCAGGAAAT 
TTGGGCGTGC TCATTCCTGT AATAGCTGTG CTCACCCGAC GATTGCCACC CATCAAAGAA 
GCTAAACCTA GGCTACAGAA GCTTTTCCGA GATTTCTGGC TCTACTCTGT GCTAATGGGA 
TTCGCTGTGG AAGGCTCAGG ACTCTGGCCG GAAGAGTGGT ATGAGGGAGT CTGTGAAATA 
GCTACCAAGT CACCTCTGCT TACCTTTCCC AGCAAAGAGC CTCTGCGATC AGTCCTCCAG 
TACAATTCAG CTATGAAAAA CGATACGGTC ACCCCTGCTG AACTGAGTGA GCTCCGAAGT 
ACCATCATCA ACTTGCTAGA TCCTCCTCCT GAGGTGTCTG CCCTCATCAA CAAGCTGGAC 
TTTGCCATGT CTACCTATCT CTTGTCTGTG TATCGGCTGG AGTACATGAG GGTACTGCGT 
TCTACAGATC CAGATCGCTT CCAGGTAATG TTCTGCTACT TTGAGGATAA AGCTATTCAA 
AAAGACAAAT CTGGGATGAT GCAGTGTGTC ATTGCTGTTG CTGACAAAGT GTTTGATGCT 
TTCCTGAACA TGATGGCAGA AAAAGCTAAG ACCAAGGAGA ATGAAGAGGA GCTGGAGCGG 
CATGCACAGT TTCTGCTGGT GAACTTCAAC CACATTCATA AGAGGATAAG GAGAGTAGCT 
GACAAATACC TATCTGGTCT GGTGGATAAG TTTCCACACT TGCTCTGGAG CGGGACTGTG 
CTGAAGACCA TGTTGGACAT CCTGCAGACC CTGTCACTAT CACTAAGTGC TGATATTCAC 
AAGGACCAAC CTTACTATGA CATTCCTGAT GCCCCCTATC GGATCACGGT TCCTGACACA 
TACGAAGCCC GGGAGAGCAT CGTGAAAGAC TTTGCTGCGC GCTGTGGAAT GATCCTACAG 
GAAGCCATGA AGTGGGCACC CACGGTCACT AAGTCCCACC TACAGGAATA TCTGAACAAG 
CATCAGAACT GGGTGTCAGG ACTGTCTCAG CACACAGGAT TGGCCATGGC CACTGAGAGC 
ATCCTTCATT TTGCCGGCTA CAACAAGCAG AACACAACTC TTGGGGCAAC TCAGCTAACT 
GAGCGTCCGG CCTGTGTGAA GAAAGACTAC TCCAACTTCA TGGCATCCTT GAATTTGCGC 
AACCGCTATG CCGGCGAGGT ACATGGAATG ATTCGGTTCT CAGGTGCCAC AGGCCAGATG 
TCTGACCTGA ACAAAATGAT GGTCCAAGAT CTGATCACAG CTTTGGACCA CAGTCATCCT 
CAGCACTATA CACAAGCCAT GTTCAAATTG ACGGCAATGC TCATTAGCAG CAAAGATTGT 
GATCCACAGC TCCTTCATCA TCTGTGTTGG GGTCCCCTCC GAATGTTCAA TGAGCATGGC 
ATGGAGACTG CACTAGCCTG CTGGGAGTGG CTGCTGGCTG GCAAGAATGG AGTGGAAGTG 
CCGTTTATGC GGGAGATGGC AGGAGCCTGG CACATGACAG TAGAGCAGAA GTTCGGCCTC 
TTTTCTGTGG AAACAAAGGA AGCAGACCCC CTGGCAGCAT CAGAAGCAAG TCAGCCAAGA 
CCTTGTCCCC CCGAAGTGAC ACCTCACTAC ATCTGGATCG ATTTCCTGGT CCAGCGGTTT 
GAGATTGCCA AGTACTGTAG CTCTGACCAG GTGGAGATCT TCTCAAGCCT GCTGCAGCGC 
TCCATGTCCC TCAATATTGG TGGAGCCAGG GGGAGCATGA ACAGGCATGT GGCAGCCATT 
GGACCACGTT TCAAACTGCT GACCTTGGGG CTGTCCCTCC TGCATGCTGA TGTGGTTCCA 
AATGCAACCA TTCGAAACGT GCTTCGAGAG AAGATATACT CAACAGCCTT TGACTATTTC 
AGTTGTCCAC CAAAGTTTCC TACTCAAGGA GAAAAGAGGC TTCGTGAAGA TATAAGCATA 
ATGATTAAGT TTTGGACTGC CATGTTCTCA GATAAGAAGT ACCTGACTGC CAGCCAGCTC 
GTTCCCCCAG ATAATCAGGA CACCCGAAGC AACTTGGATA TAACTGTGGG CTCTCGACAA 
CAGGCTACCC AGGGCTGGAT CAACACTTAC CCCCTCTCCA GTGGCATGTC AACCATCTCC 
AAAAAGTCAG GTATGTCTAA GAAAACCAAC CGGGGCTCCC AGCTGCACAA GTACTACATG 
AAACGCAGGA CACTGCTGCT GTCACTATTG GCCACTGAGA TCGAACGCCT CATCACTTGG 
TACAACCCAC TGTCAGCCCC AGAGCTAGAG CTGGACCAGG CAGGAGAGAA CAGCGTTGCC 
AACTGGAGGT CCAAGTACAT CAGCTTGAGT GAGAAGCAGT GGAAGGACAA CGTGAACCTT 
GCCTGGAGCA TCTCTCCATA CCTCGCTGTG CAGCTGCCAG CCAGGTTCAA GAATACAGAA 
GCCATTGGAA ATGAAGTGAC CCGTCTCGTT CGCTTGGATC CTGGAGCTGT TAGTGATGTC 
CCTGAAGCCA TCAAGTTCCT TGTCACCTGG CACACCATCG ATGCTGATGC TCCAGAGCTC 
AGCCATGTTT TATGCTGGGC ACCCACGGAC CCACCTACAG GCCTCTCCTA CTTCTCCAGC 
ATGTACCCAC CACACCCTCT CACTGCACAG TATGGGGTAA AAGTCCTGCG ATCCTTCCCT 
CCGGATGCCA TTCTGTTCTA CATCCCCCAG ATTGTGCAGG CCCTCAGATA TGACAAGATG 
GGCTATGTGC GTGAGTACAT TCTCTGGGCT GCAGCCAAAT CCCAGCTTCT GGCACACCAG 
TTTATTTGGA ACATGAAAAC TAACATCTAC CTTGATGAAG AAGGGCACCA GAAAGACCCT 
GACATCGGTG ACCTCCTGGA GCAGTTAGTA GAAGAGATCA CAGGGTCTCT ATCAGGCCCA 
GCCAAAGATT TCTATCAACG GGAATTTGAT TTCTTTAACA AGATCACAAA CGTGTCAGCC 
ATCATCAAGC CCTACCCTAA AGGCGATGAA AGGAAGAAGG CTTGTCTGTC AGCTCTGTCT 
GAAGTGAAGG TGCAGCCTGG CTGCTACCTG CCCAGCAACC CTGAAGCAAT CGTGCTAGAC 
ATTGACTACA AGTCAGGGAC ACCAATGCAG AGTGCCGCAA AAGCGCCGTA TCTGGCCAAG 
TTTAAGGTGA AGAGATGCGG AGTGAGTGAA CTTGAAAAAG AAGGTCTGCA GTGCCGCTCA 
GACGCAGAGG ATGAATGCTT CAGCCAAGAG GCTGATGGCA AGAAGATCTG CTGGCAAGCA 
GCCATCTTCA AAGTGGGGGA TGACTGCCGA CAGGACATGT TAGCCCTGCA GATCATCGAC 
CTCTTCAAGA ACATCTTCCA GCTGGTTGGC CTGGACCTCT TCGTTTTTCC TTATCGGGTA 
GTGGCTACTG CCCCTGGGTG CGGAGTGATT GAGTGCATCC CAGACTGTAC CTCCCGGGAC 
CAGCTGGGCC GCCAGACTGA CTTTGGCATG TATGACTACT TTACACGCCA GTATGGGGAT 
GAGTCTACCT TGGCCTTCCA GCAGGCACGC TACAACTTTA TCAGAAGCAT GGCCGCCTAT 
AGCCTCCTGC TGTTCCTGCT GCAGATCAAG GACAGGCACA ATGGCAACAT CATGCTGGAC 
AAGAAGGGGC ACATCATCCA CATCGACTTT GGCTTCATGT TTGAAAGCTC ACCGGGTGGC 
AACCTTGGCT GGGAACCAGA CATCAAGCTG ACAGACGAGA TGGTGATGAT TATGGGGGGC 
AAAATGGAAG CCACACCCTT CAAGTGGTTC ATGGAGATGT GTGTCCGAGG TTATCTGGCT 
GTGCGCCCCT ATATGGATGC AGTCGTCTCC CTGGTTACAC TCATGTTGGA CACAGGCCTG 
CCCTGTTTTC GTGGCCAGAC AATCAAACTT TTGAAACACA GGTTCAGCCC GAACATGACA 
GAGCGGGAGG CTGCGAATTT TATCATGAAG GTCATCCAGA ACTGTTTCCT CAGCAACAGG 
AGCCGGACCT ACGACATGAT CCAGTACTAC CAGAATGATA TCCCCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi4ka ( NM_001373955.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001360884.1 Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant 1, mRNA.

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CloneID	OMu52051
Clone ID Related Accession (Same CDS sequence)	NM_001373955.1 , XM_006522034.1
Accession Version	NM_001373955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-kinase alpha isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110573.11 and KY499708.1. On Sep 5, 2019 this sequence version replaced XM_006522034.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KY499708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCTG CTGGGGCCCG GGGAGGAGGC GGCGGTGGCG GCGGCGGCGG CGGCGGCGGC 
TCGGGGTCCA GCTCTGGCTC CAGCACCTCT CGAGGCTTTT ATTTCAACAC AGTACTTTCA 
CTGGCCCGCT CCCTGGCTGT GCAGAGACCA GCATCCTTGG AGAAGGTTCA AAAGCTGCTT 
TGCATGTGCC CAGTAGATTT TCATGGAATC TTCCAACTAG ATGAAAGACG GAGAGATGCA 
GTGATTGCAT TGGGCATTTT TCTCATTGAA TCTGATCTTC AGCACAAAGA TTGTGTTGTT 
CCTTATCTTC TTCGACTTCT CAGGGGTCTT CCAAAAGTGT ATTGGGTAGA AGAAAGCACA 
GCTCGGAAAG GCAGAGGTAA CCTCCCAGTT GCAGAGAGCT TCAGCTTCTG CCTGGTGACC 
TTGCTGTCTG ATGTGGCTTG CAGGGACCCC TCACTTAGGG ATGAGATTTT AGAGGCAATT 
TTGCAGGTTT TACATGTCCT TTTGGGAATG TGCCAGGCCT TGGAGATTCA AGAAAAAGAA 
TACCTTTGCA AGTATGCGAT CCCATGCCTG ATAGGAATCT CAAGATCTTT TGGACGTTAC 
AGCAATTCAG AAGAGTCTCT CCTCTCAAAG CTTTTCCCCA AAGTTTCTCC TCATTCCCTG 
AGAATCCCAG AAGAGCTAGA AGGTGTCCGA AGGCGTTCCT TTAATGACTT TCGCTCCATC 
CTGCCCAGCA ACCTGCTAAC TGTCTGTCAG GAGGGCACAC TGAAGAGGAA AACCAGCAGT 
GTGTCCAGCA TCTCTCAGGT TAGCCCAGAA CGTGGCATAC CCCCTCCCAG CTCCCCTGGA 
GGATCTGCAT TTCACTATTT TGAAGCTTCT TGCCTACCTG ATGGGACTGC CTTGGAGCCT 
GAATACTACT TCTCTACCAT CAGCTCCAGT TTTTCTATCT CTCCCCTGTT CAATGGTATT 
ACCTATAAGG AGTTCTGTAT TCCACTGGAA ATGCTTCGGG AACTCTTAAA CTTGGTGAAG 
AAGATTGTTG AGGAGCCTGT TCTCAAATCC TTAGATGCAA TTGTAGCCGG CGTGATGGAG 
GCCAATCCCA GTGCTGATCT GTACTATACA ACCTTCAGTG ATCCCCTGTA CCTGACCATG 
TTCAAGATGC TTCGAGATAC TCTGTACTAC ATGAAAGATC TGCCCACTTC CTTTGTGAAA 
GAGATCCATG ATTTTGTGTT GGAGCAGTTC AACATGAGCC AAGGGGAGCT CCAAAAGATT 
CTGCATGACG CAGATAGGAT CCACAGTGAG ATGAGCCCAC TCAAACTGCG CTGCCAAGCA 
AACGCTGCCT GTGTGGACCT CATGGTGTGG GCTGTGAAGG ATGAGCAGGG TGCAGAAAAC 
CTTTGCATCA AGCTGTCTGA AAAGCTGCAG TCCAAGACAT CCAGTAAGGT CATCATTGCC 
CATTTGCCCT TGCTGATCTG CTGCCTACAG GGTCTGGGCC GCCTGTGTGA AAGGTTCCCA 
GTGGTGGTCC ACTCAGTGAC GCCATCTTTG AGAGACTTTC TGGTAATTCC ATCCCCAGTG 
CTGGTGAAGC TCTATAAGTA CCACAGTCAG TACCACACAG TTGCTGGCAG TGATATAAAA 
ATCAGTGTCA CCAATGAGCA TTCCGAGTCA ACTCTGAATG TCTTGCCGGG CAAGAAGAAC 
CAGCCATCCA TGTATGAGCA GCTCCGAGAC ATCGCCATTG ACAACATCTG CAGGTGCCTG 
AAGGCCGGCC TCACCGTGGA CCCTGTGATC GTGGAGGCCT TCCTGGCCAG CCTCTCCAAC 
CGGCTCTACA TTTCCCAGGA GAGTGACAAG GATGCTCACT TGATTCCTGA CCATACCATC 
CGTGCCTTGG GACACATTGC AGTAGCTTTG AGGGACACTC CAAAGGTCAT GGAGCCAATT 
CTGCAGATCC TGCAGCAGAA ATTTTGCCAG CCCCCCTCAC CCCTTGATGT GCTCATCATC 
GACCAGCTGG GCTGCCTGGT GATCACTGGA AATCAATACA TTTATCAGGA AGTGTGGAAT 
CTCTTCCAGC AAATCAGTGT AAAGGCCAGT TCAGTTGTGT ATTCAGCCAC CAAAGACTAT 
AAAGACCATG GCTACAGGCA CTGCTCCCTA GCAGTGATCA ATGCCCTGGC CAACATTGCA 
GCCAACATTC AGGAGGAGCA TCTTGTGGAC GAGCTGTTGA TGAACCTGCT GGAACTGTTT 
GTGCAGCTGG GGCTGGAGGG GAAAAGAGCC AGTGAGAGGG CCAGTGAAAA GGGGCCTGCC 
CTGAAGGCTT CCAGCAGTGC AGGAAATTTG GGCGTGCTCA TTCCTGTAAT AGCTGTGCTC 
ACCCGACGAT TGCCACCCAT CAAAGAAGCT AAACCTAGGC TACAGAAGCT TTTCCGAGAT 
TTCTGGCTCT ACTCTGTGCT AATGGGATTC GCTGTGGAAG GCTCAGGACT CTGGCCGGAA 
GAGTGGTATG AGGGAGTCTG TGAAATAGCT ACCAAGTCAC CTCTGCTTAC CTTTCCCAGC 
AAAGAGCCTC TGCGATCAGT CCTCCAGTAC AATTCAGCTA TGAAAAACGA TACGGTCACC 
CCTGCTGAAC TGAGTGAGCT CCGAAGTACC ATCATCAACT TGCTAGATCC TCCTCCTGAG 
GTGTCTGCCC TCATCAACAA GCTGGACTTT GCCATGTCTA CCTATCTCTT GTCTGTGTAT 
CGGCTGGAGT ACATGAGGGT ACTGCGTTCT ACAGATCCAG ATCGCTTCCA GGTAATGTTC 
TGCTACTTTG AGGATAAAGC TATTCAAAAA GACAAATCTG GGATGATGCA GTGTGTCATT 
GCTGTTGCTG ACAAAGTGTT TGATGCTTTC CTGAACATGA TGGCAGAAAA AGCTAAGACC 
AAGGAGAATG AAGAGGAGCT GGAGCGGCAT GCACAGTTTC TGCTGGTGAA CTTCAACCAC 
ATTCATAAGA GGATAAGGAG AGTAGCTGAC AAATACCTAT CTGGTCTGGT GGATAAGTTT 
CCACACTTGC TCTGGAGCGG GACTGTGCTG AAGACCATGT TGGACATCCT GCAGACCCTG 
TCACTATCAC TAAGTGCTGA TATTCACAAG GACCAACCTT ACTATGACAT TCCTGATGCC 
CCCTATCGGA TCACGGTTCC TGACACATAC GAAGCCCGGG AGAGCATCGT GAAAGACTTT 
GCTGCGCGCT GTGGAATGAT CCTACAGGAA GCCATGAAGT GGGCACCCAC GGTCACTAAG 
TCCCACCTAC AGGAATATCT GAACAAGCAT CAGAACTGGG TGTCAGGACT GTCTCAGCAC 
ACAGGATTGG CCATGGCCAC TGAGAGCATC CTTCATTTTG CCGGCTACAA CAAGCAGAAC 
ACAACTCTTG GGGCAACTCA GCTAACTGAG CGTCCGGCCT GTGTGAAGAA AGACTACTCC 
AACTTCATGG CATCCTTGAA TTTGCGCAAC CGCTATGCCG GCGAGGTACA TGGAATGATT 
CGGTTCTCAG GTGCCACAGG CCAGATGTCT GACCTGAACA AAATGATGGT CCAAGATCTG 
ATCACAGCTT TGGACCACAG TCATCCTCAG CACTATACAC AAGCCATGTT CAAATTGACG 
GCAATGCTCA TTAGCAGCAA AGATTGTGAT CCACAGCTCC TTCATCATCT GTGTTGGGGT 
CCCCTCCGAA TGTTCAATGA GCATGGCATG GAGACTGCAC TAGCCTGCTG GGAGTGGCTG 
CTGGCTGGCA AGAATGGAGT GGAAGTGCCG TTTATGCGGG AGATGGCAGG AGCCTGGCAC 
ATGACAGTAG AGCAGAAGTT CGGCCTCTTT TCTGTGGAAA CAAAGGAAGC AGACCCCCTG 
GCAGCATCAG AAGCAAGTCA GCCAAGACCT TGTCCCCCCG AAGTGACACC TCACTACATC 
TGGATCGATT TCCTGGTCCA GCGGTTTGAG ATTGCCAAGT ACTGTAGCTC TGACCAGGTG 
GAGATCTTCT CAAGCCTGCT GCAGCGCTCC ATGTCCCTCA ATATTGGTGG AGCCAGGGGG 
AGCATGAACA GGCATGTGGC AGCCATTGGA CCACGTTTCA AACTGCTGAC CTTGGGGCTG 
TCCCTCCTGC ATGCTGATGT GGTTCCAAAT GCAACCATTC GAAACGTGCT TCGAGAGAAG 
ATATACTCAA CAGCCTTTGA CTATTTCAGT TGTCCACCAA AGTTTCCTAC TCAAGGAGAA 
AAGAGGCTTC GTGAAGATAT AAGCATAATG ATTAAGTTTT GGACTGCCAT GTTCTCAGAT 
AAGAAGTACC TGACTGCCAG CCAGCTCGTT CCCCCAGATA ATCAGGACAC CCGAAGCAAC 
TTGGATATAA CTGTGGGCTC TCGACAACAG GCTACCCAGG GCTGGATCAA CACTTACCCC 
CTCTCCAGTG GCATGTCAAC CATCTCCAAA AAGTCAGGTA TGTCTAAGAA AACCAACCGG 
GGCTCCCAGC TGCACAAGTA CTACATGAAA CGCAGGACAC TGCTGCTGTC ACTATTGGCC 
ACTGAGATCG AACGCCTCAT CACTTGGTAC AACCCACTGT CAGCCCCAGA GCTAGAGCTG 
GACCAGGCAG GAGAGAACAG CGTTGCCAAC TGGAGGTCCA AGTACATCAG CTTGAGTGAG 
AAGCAGTGGA AGGACAACGT GAACCTTGCC TGGAGCATCT CTCCATACCT CGCTGTGCAG 
CTGCCAGCCA GGTTCAAGAA TACAGAAGCC ATTGGAAATG AAGTGACCCG TCTCGTTCGC 
TTGGATCCTG GAGCTGTTAG TGATGTCCCT GAAGCCATCA AGTTCCTTGT CACCTGGCAC 
ACCATCGATG CTGATGCTCC AGAGCTCAGC CATGTTTTAT GCTGGGCACC CACGGACCCA 
CCTACAGGCC TCTCCTACTT CTCCAGCATG TACCCACCAC ACCCTCTCAC TGCACAGTAT 
GGGGTAAAAG TCCTGCGATC CTTCCCTCCG GATGCCATTC TGTTCTACAT CCCCCAGATT 
GTGCAGGCCC TCAGATATGA CAAGATGGGC TATGTGCGTG AGTACATTCT CTGGGCTGCA 
GCCAAATCCC AGCTTCTGGC ACACCAGTTT ATTTGGAACA TGAAAACTAA CATCTACCTT 
GATGAAGAAG GGCACCAGAA AGACCCTGAC ATCGGTGACC TCCTGGAGCA GTTAGTAGAA 
GAGATCACAG GGTCTCTATC AGGCCCAGCC AAAGATTTCT ATCAACGGGA ATTTGATTTC 
TTTAACAAGA TCACAAACGT GTCAGCCATC ATCAAGCCCT ACCCTAAAGG CGATGAAAGG 
AAGAAGGCTT GTCTGTCAGC TCTGTCTGAA GTGAAGGTGC AGCCTGGCTG CTACCTGCCC 
AGCAACCCTG AAGCAATCGT GCTAGACATT GACTACAAGT CAGGGACACC AATGCAGAGT 
GCCGCAAAAG CGCCGTATCT GGCCAAGTTT AAGGTGAAGA GATGCGGAGT GAGTGAACTT 
GAAAAAGAAG GTCTGCAGTG CCGCTCAGAC GCAGAGGATG AATGCTTCAG CCAAGAGGCT 
GATGGCAAGA AGATCTGCTG GCAAGCAGCC ATCTTCAAAG TGGGGGATGA CTGCCGACAG 
GACATGTTAG CCCTGCAGAT CATCGACCTC TTCAAGAACA TCTTCCAGCT GGTTGGCCTG 
GACCTCTTCG TTTTTCCTTA TCGGGTAGTG GCTACTGCCC CTGGGTGCGG AGTGATTGAG 
TGCATCCCAG ACTGTACCTC CCGGGACCAG CTGGGCCGCC AGACTGACTT TGGCATGTAT 
GACTACTTTA CACGCCAGTA TGGGGATGAG TCTACCTTGG CCTTCCAGCA GGCACGCTAC 
AACTTTATCA GAAGCATGGC CGCCTATAGC CTCCTGCTGT TCCTGCTGCA GATCAAGGAC 
AGGCACAATG GCAACATCAT GCTGGACAAG AAGGGGCACA TCATCCACAT CGACTTTGGC 
TTCATGTTTG AAAGCTCACC GGGTGGCAAC CTTGGCTGGG AACCAGACAT CAAGCTGACA 
GACGAGATGG TGATGATTAT GGGGGGCAAA ATGGAAGCCA CACCCTTCAA GTGGTTCATG 
GAGATGTGTG TCCGAGGTTA TCTGGCTGTG CGCCCCTATA TGGATGCAGT CGTCTCCCTG 
GTTACACTCA TGTTGGACAC AGGCCTGCCC TGTTTTCGTG GCCAGACAAT CAAACTTTTG 
AAACACAGGT TCAGCCCGAA CATGACAGAG CGGGAGGCTG CGAATTTTAT CATGAAGGTC 
ATCCAGAACT GTTTCCTCAG CAACAGGAGC CGGACCTACG ACATGATCCA GTACTACCAG 
AATGATATCC CCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360884.1
CDS	68..6385
Translation	MAAAGARGGGGGGGGGGGGGSGSSSGSSTSRGFYFNTVLSLARSLAVQRPASLEKVQKLLCMCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDCVVPYLLRLLRGLPKVYWVEESTARKGRGNLPVAESFSFCLVTLLSDVACRDPSLRDEILEAILQVLHVLLGMCQALEIQEKEYLCKYAIPCLIGISRSFGRYSNSEESLLSKLFPKVSPHSLRIPEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPERGIPPPSSPGGSAFHYFEASCLPDGTALEPEYYFSTISSSFSISPLFNGITYKEFCIPLEMLRELLNLVKKIVEEPVLKSLDAIVAGVMEANPSADLYYTTFSDPLYLTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFNMSQGELQKILHDADRIHSEMSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHLPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGSDIKISVTNEHSESTLNVLPGKKNQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQEEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMRVLRSTDPDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQLTERPACVKKDYSNFMASLNLRNRYAGEVHGMIRFSGATGQMSDLNKMMVQDLITALDHSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWHMTVEQKFGLFSVETKEADPLAASEASQPRPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSLNIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAAKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLEQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLQCRSDAEDECFSQEADGKKICWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQNCFLSNRSRTYDMIQYYQNDIPY

Target ORF information:

RefSeq Version	NM_001373955.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373955.1

ORF Insert Sequence:

ATGGCGGCTG CTGGGGCCCG GGGAGGAGGC GGCGGTGGCG GCGGCGGCGG CGGCGGCGGC 
TCGGGGTCCA GCTCTGGCTC CAGCACCTCT CGAGGCTTTT ATTTCAACAC AGTACTTTCA 
CTGGCCCGCT CCCTGGCTGT GCAGAGACCA GCATCCTTGG AGAAGGTTCA AAAGCTGCTT 
TGCATGTGCC CAGTAGATTT TCATGGAATC TTCCAACTAG ATGAAAGACG GAGAGATGCA 
GTGATTGCAT TGGGCATTTT TCTCATTGAA TCTGATCTTC AGCACAAAGA TTGTGTTGTT 
CCTTATCTTC TTCGACTTCT CAGGGGTCTT CCAAAAGTGT ATTGGGTAGA AGAAAGCACA 
GCTCGGAAAG GCAGAGGTAA CCTCCCAGTT GCAGAGAGCT TCAGCTTCTG CCTGGTGACC 
TTGCTGTCTG ATGTGGCTTG CAGGGACCCC TCACTTAGGG ATGAGATTTT AGAGGCAATT 
TTGCAGGTTT TACATGTCCT TTTGGGAATG TGCCAGGCCT TGGAGATTCA AGAAAAAGAA 
TACCTTTGCA AGTATGCGAT CCCATGCCTG ATAGGAATCT CAAGATCTTT TGGACGTTAC 
AGCAATTCAG AAGAGTCTCT CCTCTCAAAG CTTTTCCCCA AAGTTTCTCC TCATTCCCTG 
AGAATCCCAG AAGAGCTAGA AGGTGTCCGA AGGCGTTCCT TTAATGACTT TCGCTCCATC 
CTGCCCAGCA ACCTGCTAAC TGTCTGTCAG GAGGGCACAC TGAAGAGGAA AACCAGCAGT 
GTGTCCAGCA TCTCTCAGGT TAGCCCAGAA CGTGGCATAC CCCCTCCCAG CTCCCCTGGA 
GGATCTGCAT TTCACTATTT TGAAGCTTCT TGCCTACCTG ATGGGACTGC CTTGGAGCCT 
GAATACTACT TCTCTACCAT CAGCTCCAGT TTTTCTATCT CTCCCCTGTT CAATGGTATT 
ACCTATAAGG AGTTCTGTAT TCCACTGGAA ATGCTTCGGG AACTCTTAAA CTTGGTGAAG 
AAGATTGTTG AGGAGCCTGT TCTCAAATCC TTAGATGCAA TTGTAGCCGG CGTGATGGAG 
GCCAATCCCA GTGCTGATCT GTACTATACA ACCTTCAGTG ATCCCCTGTA CCTGACCATG 
TTCAAGATGC TTCGAGATAC TCTGTACTAC ATGAAAGATC TGCCCACTTC CTTTGTGAAA 
GAGATCCATG ATTTTGTGTT GGAGCAGTTC AACATGAGCC AAGGGGAGCT CCAAAAGATT 
CTGCATGACG CAGATAGGAT CCACAGTGAG ATGAGCCCAC TCAAACTGCG CTGCCAAGCA 
AACGCTGCCT GTGTGGACCT CATGGTGTGG GCTGTGAAGG ATGAGCAGGG TGCAGAAAAC 
CTTTGCATCA AGCTGTCTGA AAAGCTGCAG TCCAAGACAT CCAGTAAGGT CATCATTGCC 
CATTTGCCCT TGCTGATCTG CTGCCTACAG GGTCTGGGCC GCCTGTGTGA AAGGTTCCCA 
GTGGTGGTCC ACTCAGTGAC GCCATCTTTG AGAGACTTTC TGGTAATTCC ATCCCCAGTG 
CTGGTGAAGC TCTATAAGTA CCACAGTCAG TACCACACAG TTGCTGGCAG TGATATAAAA 
ATCAGTGTCA CCAATGAGCA TTCCGAGTCA ACTCTGAATG TCTTGCCGGG CAAGAAGAAC 
CAGCCATCCA TGTATGAGCA GCTCCGAGAC ATCGCCATTG ACAACATCTG CAGGTGCCTG 
AAGGCCGGCC TCACCGTGGA CCCTGTGATC GTGGAGGCCT TCCTGGCCAG CCTCTCCAAC 
CGGCTCTACA TTTCCCAGGA GAGTGACAAG GATGCTCACT TGATTCCTGA CCATACCATC 
CGTGCCTTGG GACACATTGC AGTAGCTTTG AGGGACACTC CAAAGGTCAT GGAGCCAATT 
CTGCAGATCC TGCAGCAGAA ATTTTGCCAG CCCCCCTCAC CCCTTGATGT GCTCATCATC 
GACCAGCTGG GCTGCCTGGT GATCACTGGA AATCAATACA TTTATCAGGA AGTGTGGAAT 
CTCTTCCAGC AAATCAGTGT AAAGGCCAGT TCAGTTGTGT ATTCAGCCAC CAAAGACTAT 
AAAGACCATG GCTACAGGCA CTGCTCCCTA GCAGTGATCA ATGCCCTGGC CAACATTGCA 
GCCAACATTC AGGAGGAGCA TCTTGTGGAC GAGCTGTTGA TGAACCTGCT GGAACTGTTT 
GTGCAGCTGG GGCTGGAGGG GAAAAGAGCC AGTGAGAGGG CCAGTGAAAA GGGGCCTGCC 
CTGAAGGCTT CCAGCAGTGC AGGAAATTTG GGCGTGCTCA TTCCTGTAAT AGCTGTGCTC 
ACCCGACGAT TGCCACCCAT CAAAGAAGCT AAACCTAGGC TACAGAAGCT TTTCCGAGAT 
TTCTGGCTCT ACTCTGTGCT AATGGGATTC GCTGTGGAAG GCTCAGGACT CTGGCCGGAA 
GAGTGGTATG AGGGAGTCTG TGAAATAGCT ACCAAGTCAC CTCTGCTTAC CTTTCCCAGC 
AAAGAGCCTC TGCGATCAGT CCTCCAGTAC AATTCAGCTA TGAAAAACGA TACGGTCACC 
CCTGCTGAAC TGAGTGAGCT CCGAAGTACC ATCATCAACT TGCTAGATCC TCCTCCTGAG 
GTGTCTGCCC TCATCAACAA GCTGGACTTT GCCATGTCTA CCTATCTCTT GTCTGTGTAT 
CGGCTGGAGT ACATGAGGGT ACTGCGTTCT ACAGATCCAG ATCGCTTCCA GGTAATGTTC 
TGCTACTTTG AGGATAAAGC TATTCAAAAA GACAAATCTG GGATGATGCA GTGTGTCATT 
GCTGTTGCTG ACAAAGTGTT TGATGCTTTC CTGAACATGA TGGCAGAAAA AGCTAAGACC 
AAGGAGAATG AAGAGGAGCT GGAGCGGCAT GCACAGTTTC TGCTGGTGAA CTTCAACCAC 
ATTCATAAGA GGATAAGGAG AGTAGCTGAC AAATACCTAT CTGGTCTGGT GGATAAGTTT 
CCACACTTGC TCTGGAGCGG GACTGTGCTG AAGACCATGT TGGACATCCT GCAGACCCTG 
TCACTATCAC TAAGTGCTGA TATTCACAAG GACCAACCTT ACTATGACAT TCCTGATGCC 
CCCTATCGGA TCACGGTTCC TGACACATAC GAAGCCCGGG AGAGCATCGT GAAAGACTTT 
GCTGCGCGCT GTGGAATGAT CCTACAGGAA GCCATGAAGT GGGCACCCAC GGTCACTAAG 
TCCCACCTAC AGGAATATCT GAACAAGCAT CAGAACTGGG TGTCAGGACT GTCTCAGCAC 
ACAGGATTGG CCATGGCCAC TGAGAGCATC CTTCATTTTG CCGGCTACAA CAAGCAGAAC 
ACAACTCTTG GGGCAACTCA GCTAACTGAG CGTCCGGCCT GTGTGAAGAA AGACTACTCC 
AACTTCATGG CATCCTTGAA TTTGCGCAAC CGCTATGCCG GCGAGGTACA TGGAATGATT 
CGGTTCTCAG GTGCCACAGG CCAGATGTCT GACCTGAACA AAATGATGGT CCAAGATCTG 
ATCACAGCTT TGGACCACAG TCATCCTCAG CACTATACAC AAGCCATGTT CAAATTGACG 
GCAATGCTCA TTAGCAGCAA AGATTGTGAT CCACAGCTCC TTCATCATCT GTGTTGGGGT 
CCCCTCCGAA TGTTCAATGA GCATGGCATG GAGACTGCAC TAGCCTGCTG GGAGTGGCTG 
CTGGCTGGCA AGAATGGAGT GGAAGTGCCG TTTATGCGGG AGATGGCAGG AGCCTGGCAC 
ATGACAGTAG AGCAGAAGTT CGGCCTCTTT TCTGTGGAAA CAAAGGAAGC AGACCCCCTG 
GCAGCATCAG AAGCAAGTCA GCCAAGACCT TGTCCCCCCG AAGTGACACC TCACTACATC 
TGGATCGATT TCCTGGTCCA GCGGTTTGAG ATTGCCAAGT ACTGTAGCTC TGACCAGGTG 
GAGATCTTCT CAAGCCTGCT GCAGCGCTCC ATGTCCCTCA ATATTGGTGG AGCCAGGGGG 
AGCATGAACA GGCATGTGGC AGCCATTGGA CCACGTTTCA AACTGCTGAC CTTGGGGCTG 
TCCCTCCTGC ATGCTGATGT GGTTCCAAAT GCAACCATTC GAAACGTGCT TCGAGAGAAG 
ATATACTCAA CAGCCTTTGA CTATTTCAGT TGTCCACCAA AGTTTCCTAC TCAAGGAGAA 
AAGAGGCTTC GTGAAGATAT AAGCATAATG ATTAAGTTTT GGACTGCCAT GTTCTCAGAT 
AAGAAGTACC TGACTGCCAG CCAGCTCGTT CCCCCAGATA ATCAGGACAC CCGAAGCAAC 
TTGGATATAA CTGTGGGCTC TCGACAACAG GCTACCCAGG GCTGGATCAA CACTTACCCC 
CTCTCCAGTG GCATGTCAAC CATCTCCAAA AAGTCAGGTA TGTCTAAGAA AACCAACCGG 
GGCTCCCAGC TGCACAAGTA CTACATGAAA CGCAGGACAC TGCTGCTGTC ACTATTGGCC 
ACTGAGATCG AACGCCTCAT CACTTGGTAC AACCCACTGT CAGCCCCAGA GCTAGAGCTG 
GACCAGGCAG GAGAGAACAG CGTTGCCAAC TGGAGGTCCA AGTACATCAG CTTGAGTGAG 
AAGCAGTGGA AGGACAACGT GAACCTTGCC TGGAGCATCT CTCCATACCT CGCTGTGCAG 
CTGCCAGCCA GGTTCAAGAA TACAGAAGCC ATTGGAAATG AAGTGACCCG TCTCGTTCGC 
TTGGATCCTG GAGCTGTTAG TGATGTCCCT GAAGCCATCA AGTTCCTTGT CACCTGGCAC 
ACCATCGATG CTGATGCTCC AGAGCTCAGC CATGTTTTAT GCTGGGCACC CACGGACCCA 
CCTACAGGCC TCTCCTACTT CTCCAGCATG TACCCACCAC ACCCTCTCAC TGCACAGTAT 
GGGGTAAAAG TCCTGCGATC CTTCCCTCCG GATGCCATTC TGTTCTACAT CCCCCAGATT 
GTGCAGGCCC TCAGATATGA CAAGATGGGC TATGTGCGTG AGTACATTCT CTGGGCTGCA 
GCCAAATCCC AGCTTCTGGC ACACCAGTTT ATTTGGAACA TGAAAACTAA CATCTACCTT 
GATGAAGAAG GGCACCAGAA AGACCCTGAC ATCGGTGACC TCCTGGAGCA GTTAGTAGAA 
GAGATCACAG GGTCTCTATC AGGCCCAGCC AAAGATTTCT ATCAACGGGA ATTTGATTTC 
TTTAACAAGA TCACAAACGT GTCAGCCATC ATCAAGCCCT ACCCTAAAGG CGATGAAAGG 
AAGAAGGCTT GTCTGTCAGC TCTGTCTGAA GTGAAGGTGC AGCCTGGCTG CTACCTGCCC 
AGCAACCCTG AAGCAATCGT GCTAGACATT GACTACAAGT CAGGGACACC AATGCAGAGT 
GCCGCAAAAG CGCCGTATCT GGCCAAGTTT AAGGTGAAGA GATGCGGAGT GAGTGAACTT 
GAAAAAGAAG GTCTGCAGTG CCGCTCAGAC GCAGAGGATG AATGCTTCAG CCAAGAGGCT 
GATGGCAAGA AGATCTGCTG GCAAGCAGCC ATCTTCAAAG TGGGGGATGA CTGCCGACAG 
GACATGTTAG CCCTGCAGAT CATCGACCTC TTCAAGAACA TCTTCCAGCT GGTTGGCCTG 
GACCTCTTCG TTTTTCCTTA TCGGGTAGTG GCTACTGCCC CTGGGTGCGG AGTGATTGAG 
TGCATCCCAG ACTGTACCTC CCGGGACCAG CTGGGCCGCC AGACTGACTT TGGCATGTAT 
GACTACTTTA CACGCCAGTA TGGGGATGAG TCTACCTTGG CCTTCCAGCA GGCACGCTAC 
AACTTTATCA GAAGCATGGC CGCCTATAGC CTCCTGCTGT TCCTGCTGCA GATCAAGGAC 
AGGCACAATG GCAACATCAT GCTGGACAAG AAGGGGCACA TCATCCACAT CGACTTTGGC 
TTCATGTTTG AAAGCTCACC GGGTGGCAAC CTTGGCTGGG AACCAGACAT CAAGCTGACA 
GACGAGATGG TGATGATTAT GGGGGGCAAA ATGGAAGCCA CACCCTTCAA GTGGTTCATG 
GAGATGTGTG TCCGAGGTTA TCTGGCTGTG CGCCCCTATA TGGATGCAGT CGTCTCCCTG 
GTTACACTCA TGTTGGACAC AGGCCTGCCC TGTTTTCGTG GCCAGACAAT CAAACTTTTG 
AAACACAGGT TCAGCCCGAA CATGACAGAG CGGGAGGCTG CGAATTTTAT CATGAAGGTC 
ATCCAGAACT GTTTCCTCAG CAACAGGAGC CGGACCTACG ACATGATCCA GTACTACCAG 
AATGATATCC CCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi4ka ( NM_001001983.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001001983.2 Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant 2, mRNA.

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CloneID	OMu19021
Clone ID Related Accession (Same CDS sequence)	NM_001001983.2
Accession Version	NM_001001983.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-kinase alpha isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC075629.1, CF747564.1 and BU700531.1. On Jun 25, 2009 this sequence version replaced NM_001001983.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC075629.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGCCCAG TAGATTTTCA TGGAATCTTC CAACTAGATG AAAGACGGAG AGATGCAGTG 
ATTGCATTGG GCATTTTTCT CATTGAATCT GATCTTCAGC ACAAAGATTG TGTTGTTCCT 
TATCTTCTTC GACTTCTCAG GGGTCTTCCA AAAGTGTATT GGGTAGAAGA AAGCACAGCT 
CGGAAAGGCA GAGGTAACCT CCCAGTTGCA GAGAGCTTCA GCTTCTGCCT GGTGACCTTG 
CTGTCTGATG TGGCTTGCAG GGACCCCTCA CTTAGGGATG AGATTTTAGA GGCAATTTTG 
CAGGTTTTAC ATGTCCTTTT GGGAATGTGC CAGGCCTTGG AGATTCAAGA AAAAGAATAC 
CTTTGCAAGT ATGCGATCCC ATGCCTGATA GGAATCTCAA GATCTTTTGG ACGTTACAGC 
AATTCAGAAG AGTCTCTCCT CTCAAAGCTT TTCCCCAAAG TTTCTCCTCA TTCCCTGAGA 
ATCCCAGAAG AGCTAGAAGG TGTCCGAAGG CGTTCCTTTA ATGACTTTCG CTCCATCCTG 
CCCAGCAACC TGCTAACTGT CTGTCAGGAG GGCACACTGA AGAGGAAAAC CAGCAGTGTG 
TCCAGCATCT CTCAGGTTAG CCCAGAACGT GGCATACCCC CTCCCAGCTC CCCTGGAGGA 
TCTGCATTTC ACTATTTTGA AGCTTCTTGC CTACCTGATG GGACTGCCTT GGAGCCTGAA 
TACTACTTCT CTACCATCAG CTCCAGTTTT TCTATCTCTC CCCTGTTCAA TGGTATTACC 
TATAAGGAGT TCTGTATTCC ACTGGAAATG CTTCGGGAAC TCTTAAACTT GGTGAAGAAG 
ATTGTTGAGG AGCCTGTTCT CAAATCCTTA GATGCAATTG TAGCCGGCGT GATGGAGGCC 
AATCCCAGTG CTGATCTGTA CTATACAACC TTCAGTGATC CCCTGTACCT GACCATGTTC 
AAGATGCTTC GAGATACTCT GTACTACATG AAAGATCTGC CCACTTCCTT TGTGAAAGAG 
ATCCATGATT TTGTGTTGGA GCAGTTCAAC ATGAGCCAAG GGGAGCTCCA AAAGATTCTG 
CATGACGCAG ATAGGATCCA CAGTGAGATG AGCCCACTCA AACTGCGCTG CCAAGCAAAC 
GCTGCCTGTG TGGACCTCAT GGTGTGGGCT GTGAAGGATG AGCAGGGTGC AGAAAACCTT 
TGCATCAAGC TGTCTGAAAA GCTGCAGTCC AAGACATCCA GTAAGGTCAT CATTGCCCAT 
TTGCCCTTGC TGATCTGCTG CCTACAGGGT CTGGGCCGCC TGTGTGAAAG GTTCCCAGTG 
GTGGTCCACT CAGTGACGCC ATCTTTGAGA GACTTTCTGG TAATTCCATC CCCAGTGCTG 
GTGAAGCTCT ATAAGTACCA CAGTCAGTAC CACACAGTTG CTGGCAGTGA TATAAAAATC 
AGTGTCACCA ATGAGCATTC CGAGTCAACT CTGAATGTCT TGCCGGGCAA GAAGAACCAG 
CCATCCATGT ATGAGCAGCT CCGAGACATC GCCATTGACA ACATCTGCAG GTGCCTGAAG 
GCCGGCCTCA CCGTGGACCC TGTGATCGTG GAGGCCTTCC TGGCCAGCCT CTCCAACCGG 
CTCTACATTT CCCAGGAGAG TGACAAGGAT GCTCACTTGA TTCCTGACCA TACCATCCGT 
GCCTTGGGAC ACATTGCAGT AGCTTTGAGG GACACTCCAA AGGTCATGGA GCCAATTCTG 
CAGATCCTGC AGCAGAAATT TTGCCAGCCC CCCTCACCCC TTGATGTGCT CATCATCGAC 
CAGCTGGGCT GCCTGGTGAT CACTGGAAAT CAATACATTT ATCAGGAAGT GTGGAATCTC 
TTCCAGCAAA TCAGTGTAAA GGCCAGTTCA GTTGTGTATT CAGCCACCAA AGACTATAAA 
GACCATGGCT ACAGGCACTG CTCCCTAGCA GTGATCAATG CCCTGGCCAA CATTGCAGCC 
AACATTCAGG AGGAGCATCT TGTGGACGAG CTGTTGATGA ACCTGCTGGA ACTGTTTGTG 
CAGCTGGGGC TGGAGGGGAA AAGAGCCAGT GAGAGGGCCA GTGAAAAGGG GCCTGCCCTG 
AAGGCTTCCA GCAGTGCAGG AAATTTGGGC GTGCTCATTC CTGTAATAGC TGTGCTCACC 
CGACGATTGC CACCCATCAA AGAAGCTAAA CCTAGGCTAC AGAAGCTTTT CCGAGATTTC 
TGGCTCTACT CTGTGCTAAT GGGATTCGCT GTGGAAGGCT CAGGACTCTG GCCGGAAGAG 
TGGTATGAGG GAGTCTGTGA AATAGCTACC AAGTCACCTC TGCTTACCTT TCCCAGCAAA 
GAGCCTCTGC GATCAGTCCT CCAGTACAAT TCAGCTATGA AAAACGATAC GGTCACCCCT 
GCTGAACTGA GTGAGCTCCG AAGTACCATC ATCAACTTGC TAGATCCTCC TCCTGAGGTG 
TCTGCCCTCA TCAACAAGCT GGACTTTGCC ATGTCTACCT ATCTCTTGTC TGTGTATCGG 
CTGGAGTACA TGAGGGTACT GCGTTCTACA GATCCAGATC GCTTCCAGGT AATGTTCTGC 
TACTTTGAGG ATAAAGCTAT TCAAAAAGAC AAATCTGGGA TGATGCAGTG TGTCATTGCT 
GTTGCTGACA AAGTGTTTGA TGCTTTCCTG AACATGATGG CAGAAAAAGC TAAGACCAAG 
GAGAATGAAG AGGAGCTGGA GCGGCATGCA CAGTTTCTGC TGGTGAACTT CAACCACATT 
CATAAGAGGA TAAGGAGAGT AGCTGACAAA TACCTATCTG GTCTGGTGGA TAAGTTTCCA 
CACTTGCTCT GGAGCGGGAC TGTGCTGAAG ACCATGTTGG ACATCCTGCA GACCCTGTCA 
CTATCACTAA GTGCTGATAT TCACAAGGAC CAACCTTACT ATGACATTCC TGATGCCCCC 
TATCGGATCA CGGTTCCTGA CACATACGAA GCCCGGGAGA GCATCGTGAA AGACTTTGCT 
GCGCGCTGTG GAATGATCCT ACAGGAAGCC ATGAAGTGGG CACCCACGGT CACTAAGTCC 
CACCTACAGG AATATCTGAA CAAGCATCAG AACTGGGTGT CAGGACTGTC TCAGCACACA 
GGATTGGCCA TGGCCACTGA GAGCATCCTT CATTTTGCCG GCTACAACAA GCAGAACACA 
ACTCTTGGGG CAACTCAGCT AACTGAGCGT CCGGCCTGTG TGAAGAAAGA CTACTCCAAC 
TTCATGGCAT CCTTGAATTT GCGCAACCGC TATGCCGGCG AGGTACATGG AATGATTCGG 
TTCTCAGGTG CCACAGGCCA GATGTCTGAC CTGAACAAAA TGATGGTCCA AGATCTGATC 
ACAGCTTTGG ACCACAGTCA TCCTCAGCAC TATACACAAG CCATGTTCAA ATTGACGGCA 
ATGCTCATTA GCAGCAAAGA TTGTGATCCA CAGCTCCTTC ATCATCTGTG TTGGGGTCCC 
CTCCGAATGT TCAATGAGCA TGGCATGGAG ACTGCACTAG CCTGCTGGGA GTGGCTGCTG 
GCTGGCAAGA ATGGAGTGGA AGTGCCGTTT ATGCGGGAGA TGGCAGGAGC CTGGCACATG 
ACAGTAGAGC AGAAGTTCGG CCTCTTTTCT GTGGAAACAA AGGAAGCAGA CCCCCTGGCA 
GCATCAGAAG CAAGTCAGCC AAGACCTTGT CCCCCCGAAG TGACACCTCA CTACATCTGG 
ATCGATTTCC TGGTCCAGCG GTTTGAGATT GCCAAGTACT GTAGCTCTGA CCAGGTGGAG 
ATCTTCTCAA GCCTGCTGCA GCGCTCCATG TCCCTCAATA TTGGTGGAGC CAGGGGGAGC 
ATGAACAGGC ATGTGGCAGC CATTGGACCA CGTTTCAAAC TGCTGACCTT GGGGCTGTCC 
CTCCTGCATG CTGATGTGGT TCCAAATGCA ACCATTCGAA ACGTGCTTCG AGAGAAGATA 
TACTCAACAG CCTTTGACTA TTTCAGTTGT CCACCAAAGT TTCCTACTCA AGGAGAAAAG 
AGGCTTCGTG AAGATATAAG CATAATGATT AAGTTTTGGA CTGCCATGTT CTCAGATAAG 
AAGTACCTGA CTGCCAGCCA GCTCGTTCCC CCAGATAATC AGGACACCCG AAGCAACTTG 
GATATAACTG TGGGCTCTCG ACAACAGGCT ACCCAGGGCT GGATCAACAC TTACCCCCTC 
TCCAGTGGCA TGTCAACCAT CTCCAAAAAG TCAGGTATGT CTAAGAAAAC CAACCGGGGC 
TCCCAGCTGC ACAAGTACTA CATGAAACGC AGGACACTGC TGCTGTCACT ATTGGCCACT 
GAGATCGAAC GCCTCATCAC TTGGTACAAC CCACTGTCAG CCCCAGAGCT AGAGCTGGAC 
CAGGCAGGAG AGAACAGCGT TGCCAACTGG AGGTCCAAGT ACATCAGCTT GAGTGAGAAG 
CAGTGGAAGG ACAACGTGAA CCTTGCCTGG AGCATCTCTC CATACCTCGC TGTGCAGCTG 
CCAGCCAGGT TCAAGAATAC AGAAGCCATT GGAAATGAAG TGACCCGTCT CGTTCGCTTG 
GATCCTGGAG CTGTTAGTGA TGTCCCTGAA GCCATCAAGT TCCTTGTCAC CTGGCACACC 
ATCGATGCTG ATGCTCCAGA GCTCAGCCAT GTTTTATGCT GGGCACCCAC GGACCCACCT 
ACAGGCCTCT CCTACTTCTC CAGCATGTAC CCACCACACC CTCTCACTGC ACAGTATGGG 
GTAAAAGTCC TGCGATCCTT CCCTCCGGAT GCCATTCTGT TCTACATCCC CCAGATTGTG 
CAGGCCCTCA GATATGACAA GATGGGCTAT GTGCGTGAGT ACATTCTCTG GGCTGCAGCC 
AAATCCCAGC TTCTGGCACA CCAGTTTATT TGGAACATGA AAACTAACAT CTACCTTGAT 
GAAGAAGGGC ACCAGAAAGA CCCTGACATC GGTGACCTCC TGGAGCAGTT AGTAGAAGAG 
ATCACAGGGT CTCTATCAGG CCCAGCCAAA GATTTCTATC AACGGGAATT TGATTTCTTT 
AACAAGATCA CAAACGTGTC AGCCATCATC AAGCCCTACC CTAAAGGCGA TGAAAGGAAG 
AAGGCTTGTC TGTCAGCTCT GTCTGAAGTG AAGGTGCAGC CTGGCTGCTA CCTGCCCAGC 
AACCCTGAAG CAATCGTGCT AGACATTGAC TACAAGTCAG GGACACCAAT GCAGAGTGCC 
GCAAAAGCGC CGTATCTGGC CAAGTTTAAG GTGAAGAGAT GCGGAGTGAG TGAACTTGAA 
AAAGAAGGTC TGCAGTGCCG CTCAGACGCA GAGGATGAAT GCTTCAGCCA AGAGGCTGAT 
GGCAAGAAGA TCTGCTGGCA AGCAGCCATC TTCAAAGTGG GGGATGACTG CCGACAGGAC 
ATGTTAGCCC TGCAGATCAT CGACCTCTTC AAGAACATCT TCCAGCTGGT TGGCCTGGAC 
CTCTTCGTTT TTCCTTATCG GGTAGTGGCT ACTGCCCCTG GGTGCGGAGT GATTGAGTGC 
ATCCCAGACT GTACCTCCCG GGACCAGCTG GGCCGCCAGA CTGACTTTGG CATGTATGAC 
TACTTTACAC GCCAGTATGG GGATGAGTCT ACCTTGGCCT TCCAGCAGGC ACGCTACAAC 
TTTATCAGAA GCATGGCCGC CTATAGCCTC CTGCTGTTCC TGCTGCAGAT CAAGGACAGG 
CACAATGGCA ACATCATGCT GGACAAGAAG GGGCACATCA TCCACATCGA CTTTGGCTTC 
ATGTTTGAAA GCTCACCGGG TGGCAACCTT GGCTGGGAAC CAGACATCAA GCTGACAGAC 
GAGATGGTGA TGATTATGGG GGGCAAAATG GAAGCCACAC CCTTCAAGTG GTTCATGGAG 
ATGTGTGTCC GAGGTTATCT GGCTGTGCGC CCCTATATGG ATGCAGTCGT CTCCCTGGTT 
ACACTCATGT TGGACACAGG CCTGCCCTGT TTTCGTGGCC AGACAATCAA ACTTTTGAAA 
CACAGGTTCA GCCCGAACAT GACAGAGCGG GAGGCTGCGA ATTTTATCAT GAAGGTCATC 
CAGAACTGTT TCCTCAGCAA CAGGAGCCGG ACCTACGACA TGATCCAGTA CTACCAGAAT 
GATATCCCCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001983.2
CDS	79..6213
Translation	MCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDCVVPYLLRLLRGLPKVYWVEESTARKGRGNLPVAESFSFCLVTLLSDVACRDPSLRDEILEAILQVLHVLLGMCQALEIQEKEYLCKYAIPCLIGISRSFGRYSNSEESLLSKLFPKVSPHSLRIPEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPERGIPPPSSPGGSAFHYFEASCLPDGTALEPEYYFSTISSSFSISPLFNGITYKEFCIPLEMLRELLNLVKKIVEEPVLKSLDAIVAGVMEANPSADLYYTTFSDPLYLTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFNMSQGELQKILHDADRIHSEMSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHLPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGSDIKISVTNEHSESTLNVLPGKKNQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQEEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMRVLRSTDPDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQLTERPACVKKDYSNFMASLNLRNRYAGEVHGMIRFSGATGQMSDLNKMMVQDLITALDHSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWHMTVEQKFGLFSVETKEADPLAASEASQPRPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSLNIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAAKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLEQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLQCRSDAEDECFSQEADGKKICWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQNCFLSNRSRTYDMIQYYQNDIPY

Target ORF information:

RefSeq Version	NM_001001983.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol 4-kinase alpha (Pi4ka), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001983.2

ORF Insert Sequence:

ATGTGCCCAG TAGATTTTCA TGGAATCTTC CAACTAGATG AAAGACGGAG AGATGCAGTG 
ATTGCATTGG GCATTTTTCT CATTGAATCT GATCTTCAGC ACAAAGATTG TGTTGTTCCT 
TATCTTCTTC GACTTCTCAG GGGTCTTCCA AAAGTGTATT GGGTAGAAGA AAGCACAGCT 
CGGAAAGGCA GAGGTAACCT CCCAGTTGCA GAGAGCTTCA GCTTCTGCCT GGTGACCTTG 
CTGTCTGATG TGGCTTGCAG GGACCCCTCA CTTAGGGATG AGATTTTAGA GGCAATTTTG 
CAGGTTTTAC ATGTCCTTTT GGGAATGTGC CAGGCCTTGG AGATTCAAGA AAAAGAATAC 
CTTTGCAAGT ATGCGATCCC ATGCCTGATA GGAATCTCAA GATCTTTTGG ACGTTACAGC 
AATTCAGAAG AGTCTCTCCT CTCAAAGCTT TTCCCCAAAG TTTCTCCTCA TTCCCTGAGA 
ATCCCAGAAG AGCTAGAAGG TGTCCGAAGG CGTTCCTTTA ATGACTTTCG CTCCATCCTG 
CCCAGCAACC TGCTAACTGT CTGTCAGGAG GGCACACTGA AGAGGAAAAC CAGCAGTGTG 
TCCAGCATCT CTCAGGTTAG CCCAGAACGT GGCATACCCC CTCCCAGCTC CCCTGGAGGA 
TCTGCATTTC ACTATTTTGA AGCTTCTTGC CTACCTGATG GGACTGCCTT GGAGCCTGAA 
TACTACTTCT CTACCATCAG CTCCAGTTTT TCTATCTCTC CCCTGTTCAA TGGTATTACC 
TATAAGGAGT TCTGTATTCC ACTGGAAATG CTTCGGGAAC TCTTAAACTT GGTGAAGAAG 
ATTGTTGAGG AGCCTGTTCT CAAATCCTTA GATGCAATTG TAGCCGGCGT GATGGAGGCC 
AATCCCAGTG CTGATCTGTA CTATACAACC TTCAGTGATC CCCTGTACCT GACCATGTTC 
AAGATGCTTC GAGATACTCT GTACTACATG AAAGATCTGC CCACTTCCTT TGTGAAAGAG 
ATCCATGATT TTGTGTTGGA GCAGTTCAAC ATGAGCCAAG GGGAGCTCCA AAAGATTCTG 
CATGACGCAG ATAGGATCCA CAGTGAGATG AGCCCACTCA AACTGCGCTG CCAAGCAAAC 
GCTGCCTGTG TGGACCTCAT GGTGTGGGCT GTGAAGGATG AGCAGGGTGC AGAAAACCTT 
TGCATCAAGC TGTCTGAAAA GCTGCAGTCC AAGACATCCA GTAAGGTCAT CATTGCCCAT 
TTGCCCTTGC TGATCTGCTG CCTACAGGGT CTGGGCCGCC TGTGTGAAAG GTTCCCAGTG 
GTGGTCCACT CAGTGACGCC ATCTTTGAGA GACTTTCTGG TAATTCCATC CCCAGTGCTG 
GTGAAGCTCT ATAAGTACCA CAGTCAGTAC CACACAGTTG CTGGCAGTGA TATAAAAATC 
AGTGTCACCA ATGAGCATTC CGAGTCAACT CTGAATGTCT TGCCGGGCAA GAAGAACCAG 
CCATCCATGT ATGAGCAGCT CCGAGACATC GCCATTGACA ACATCTGCAG GTGCCTGAAG 
GCCGGCCTCA CCGTGGACCC TGTGATCGTG GAGGCCTTCC TGGCCAGCCT CTCCAACCGG 
CTCTACATTT CCCAGGAGAG TGACAAGGAT GCTCACTTGA TTCCTGACCA TACCATCCGT 
GCCTTGGGAC ACATTGCAGT AGCTTTGAGG GACACTCCAA AGGTCATGGA GCCAATTCTG 
CAGATCCTGC AGCAGAAATT TTGCCAGCCC CCCTCACCCC TTGATGTGCT CATCATCGAC 
CAGCTGGGCT GCCTGGTGAT CACTGGAAAT CAATACATTT ATCAGGAAGT GTGGAATCTC 
TTCCAGCAAA TCAGTGTAAA GGCCAGTTCA GTTGTGTATT CAGCCACCAA AGACTATAAA 
GACCATGGCT ACAGGCACTG CTCCCTAGCA GTGATCAATG CCCTGGCCAA CATTGCAGCC 
AACATTCAGG AGGAGCATCT TGTGGACGAG CTGTTGATGA ACCTGCTGGA ACTGTTTGTG 
CAGCTGGGGC TGGAGGGGAA AAGAGCCAGT GAGAGGGCCA GTGAAAAGGG GCCTGCCCTG 
AAGGCTTCCA GCAGTGCAGG AAATTTGGGC GTGCTCATTC CTGTAATAGC TGTGCTCACC 
CGACGATTGC CACCCATCAA AGAAGCTAAA CCTAGGCTAC AGAAGCTTTT CCGAGATTTC 
TGGCTCTACT CTGTGCTAAT GGGATTCGCT GTGGAAGGCT CAGGACTCTG GCCGGAAGAG 
TGGTATGAGG GAGTCTGTGA AATAGCTACC AAGTCACCTC TGCTTACCTT TCCCAGCAAA 
GAGCCTCTGC GATCAGTCCT CCAGTACAAT TCAGCTATGA AAAACGATAC GGTCACCCCT 
GCTGAACTGA GTGAGCTCCG AAGTACCATC ATCAACTTGC TAGATCCTCC TCCTGAGGTG 
TCTGCCCTCA TCAACAAGCT GGACTTTGCC ATGTCTACCT ATCTCTTGTC TGTGTATCGG 
CTGGAGTACA TGAGGGTACT GCGTTCTACA GATCCAGATC GCTTCCAGGT AATGTTCTGC 
TACTTTGAGG ATAAAGCTAT TCAAAAAGAC AAATCTGGGA TGATGCAGTG TGTCATTGCT 
GTTGCTGACA AAGTGTTTGA TGCTTTCCTG AACATGATGG CAGAAAAAGC TAAGACCAAG 
GAGAATGAAG AGGAGCTGGA GCGGCATGCA CAGTTTCTGC TGGTGAACTT CAACCACATT 
CATAAGAGGA TAAGGAGAGT AGCTGACAAA TACCTATCTG GTCTGGTGGA TAAGTTTCCA 
CACTTGCTCT GGAGCGGGAC TGTGCTGAAG ACCATGTTGG ACATCCTGCA GACCCTGTCA 
CTATCACTAA GTGCTGATAT TCACAAGGAC CAACCTTACT ATGACATTCC TGATGCCCCC 
TATCGGATCA CGGTTCCTGA CACATACGAA GCCCGGGAGA GCATCGTGAA AGACTTTGCT 
GCGCGCTGTG GAATGATCCT ACAGGAAGCC ATGAAGTGGG CACCCACGGT CACTAAGTCC 
CACCTACAGG AATATCTGAA CAAGCATCAG AACTGGGTGT CAGGACTGTC TCAGCACACA 
GGATTGGCCA TGGCCACTGA GAGCATCCTT CATTTTGCCG GCTACAACAA GCAGAACACA 
ACTCTTGGGG CAACTCAGCT AACTGAGCGT CCGGCCTGTG TGAAGAAAGA CTACTCCAAC 
TTCATGGCAT CCTTGAATTT GCGCAACCGC TATGCCGGCG AGGTACATGG AATGATTCGG 
TTCTCAGGTG CCACAGGCCA GATGTCTGAC CTGAACAAAA TGATGGTCCA AGATCTGATC 
ACAGCTTTGG ACCACAGTCA TCCTCAGCAC TATACACAAG CCATGTTCAA ATTGACGGCA 
ATGCTCATTA GCAGCAAAGA TTGTGATCCA CAGCTCCTTC ATCATCTGTG TTGGGGTCCC 
CTCCGAATGT TCAATGAGCA TGGCATGGAG ACTGCACTAG CCTGCTGGGA GTGGCTGCTG 
GCTGGCAAGA ATGGAGTGGA AGTGCCGTTT ATGCGGGAGA TGGCAGGAGC CTGGCACATG 
ACAGTAGAGC AGAAGTTCGG CCTCTTTTCT GTGGAAACAA AGGAAGCAGA CCCCCTGGCA 
GCATCAGAAG CAAGTCAGCC AAGACCTTGT CCCCCCGAAG TGACACCTCA CTACATCTGG 
ATCGATTTCC TGGTCCAGCG GTTTGAGATT GCCAAGTACT GTAGCTCTGA CCAGGTGGAG 
ATCTTCTCAA GCCTGCTGCA GCGCTCCATG TCCCTCAATA TTGGTGGAGC CAGGGGGAGC 
ATGAACAGGC ATGTGGCAGC CATTGGACCA CGTTTCAAAC TGCTGACCTT GGGGCTGTCC 
CTCCTGCATG CTGATGTGGT TCCAAATGCA ACCATTCGAA ACGTGCTTCG AGAGAAGATA 
TACTCAACAG CCTTTGACTA TTTCAGTTGT CCACCAAAGT TTCCTACTCA AGGAGAAAAG 
AGGCTTCGTG AAGATATAAG CATAATGATT AAGTTTTGGA CTGCCATGTT CTCAGATAAG 
AAGTACCTGA CTGCCAGCCA GCTCGTTCCC CCAGATAATC AGGACACCCG AAGCAACTTG 
GATATAACTG TGGGCTCTCG ACAACAGGCT ACCCAGGGCT GGATCAACAC TTACCCCCTC 
TCCAGTGGCA TGTCAACCAT CTCCAAAAAG TCAGGTATGT CTAAGAAAAC CAACCGGGGC 
TCCCAGCTGC ACAAGTACTA CATGAAACGC AGGACACTGC TGCTGTCACT ATTGGCCACT 
GAGATCGAAC GCCTCATCAC TTGGTACAAC CCACTGTCAG CCCCAGAGCT AGAGCTGGAC 
CAGGCAGGAG AGAACAGCGT TGCCAACTGG AGGTCCAAGT ACATCAGCTT GAGTGAGAAG 
CAGTGGAAGG ACAACGTGAA CCTTGCCTGG AGCATCTCTC CATACCTCGC TGTGCAGCTG 
CCAGCCAGGT TCAAGAATAC AGAAGCCATT GGAAATGAAG TGACCCGTCT CGTTCGCTTG 
GATCCTGGAG CTGTTAGTGA TGTCCCTGAA GCCATCAAGT TCCTTGTCAC CTGGCACACC 
ATCGATGCTG ATGCTCCAGA GCTCAGCCAT GTTTTATGCT GGGCACCCAC GGACCCACCT 
ACAGGCCTCT CCTACTTCTC CAGCATGTAC CCACCACACC CTCTCACTGC ACAGTATGGG 
GTAAAAGTCC TGCGATCCTT CCCTCCGGAT GCCATTCTGT TCTACATCCC CCAGATTGTG 
CAGGCCCTCA GATATGACAA GATGGGCTAT GTGCGTGAGT ACATTCTCTG GGCTGCAGCC 
AAATCCCAGC TTCTGGCACA CCAGTTTATT TGGAACATGA AAACTAACAT CTACCTTGAT 
GAAGAAGGGC ACCAGAAAGA CCCTGACATC GGTGACCTCC TGGAGCAGTT AGTAGAAGAG 
ATCACAGGGT CTCTATCAGG CCCAGCCAAA GATTTCTATC AACGGGAATT TGATTTCTTT 
AACAAGATCA CAAACGTGTC AGCCATCATC AAGCCCTACC CTAAAGGCGA TGAAAGGAAG 
AAGGCTTGTC TGTCAGCTCT GTCTGAAGTG AAGGTGCAGC CTGGCTGCTA CCTGCCCAGC 
AACCCTGAAG CAATCGTGCT AGACATTGAC TACAAGTCAG GGACACCAAT GCAGAGTGCC 
GCAAAAGCGC CGTATCTGGC CAAGTTTAAG GTGAAGAGAT GCGGAGTGAG TGAACTTGAA 
AAAGAAGGTC TGCAGTGCCG CTCAGACGCA GAGGATGAAT GCTTCAGCCA AGAGGCTGAT 
GGCAAGAAGA TCTGCTGGCA AGCAGCCATC TTCAAAGTGG GGGATGACTG CCGACAGGAC 
ATGTTAGCCC TGCAGATCAT CGACCTCTTC AAGAACATCT TCCAGCTGGT TGGCCTGGAC 
CTCTTCGTTT TTCCTTATCG GGTAGTGGCT ACTGCCCCTG GGTGCGGAGT GATTGAGTGC 
ATCCCAGACT GTACCTCCCG GGACCAGCTG GGCCGCCAGA CTGACTTTGG CATGTATGAC 
TACTTTACAC GCCAGTATGG GGATGAGTCT ACCTTGGCCT TCCAGCAGGC ACGCTACAAC 
TTTATCAGAA GCATGGCCGC CTATAGCCTC CTGCTGTTCC TGCTGCAGAT CAAGGACAGG 
CACAATGGCA ACATCATGCT GGACAAGAAG GGGCACATCA TCCACATCGA CTTTGGCTTC 
ATGTTTGAAA GCTCACCGGG TGGCAACCTT GGCTGGGAAC CAGACATCAA GCTGACAGAC 
GAGATGGTGA TGATTATGGG GGGCAAAATG GAAGCCACAC CCTTCAAGTG GTTCATGGAG 
ATGTGTGTCC GAGGTTATCT GGCTGTGCGC CCCTATATGG ATGCAGTCGT CTCCCTGGTT 
ACACTCATGT TGGACACAGG CCTGCCCTGT TTTCGTGGCC AGACAATCAA ACTTTTGAAA 
CACAGGTTCA GCCCGAACAT GACAGAGCGG GAGGCTGCGA ATTTTATCAT GAAGGTCATC 
CAGAACTGTT TCCTCAGCAA CAGGAGCCGG ACCTACGACA TGATCCAGTA CTACCAGAAT 
GATATCCCCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi4ka ( XM_006522034.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522097.1 Mus musculus phosphatidylinositol 4-kinase, catalytic, alpha polypeptide (Pi4ka), transcript variant X1, mRNA.

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CloneID	OMu52051
Clone ID Related Accession (Same CDS sequence)	NM_001373955.1 , XM_006522034.1
Accession Version	XM_006522034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CTGGGGCCCG GGGAGGAGGC GGCGGTGGCG GCGGCGGCGG CGGCGGCGGC 
TCGGGGTCCA GCTCTGGCTC CAGCACCTCT CGAGGCTTTT ATTTCAACAC AGTACTTTCA 
CTGGCCCGCT CCCTGGCTGT GCAGAGACCA GCATCCTTGG AGAAGGTTCA AAAGCTGCTT 
TGCATGTGCC CAGTAGATTT TCATGGAATC TTCCAACTAG ATGAAAGACG GAGAGATGCA 
GTGATTGCAT TGGGCATTTT TCTCATTGAA TCTGATCTTC AGCACAAAGA TTGTGTTGTT 
CCTTATCTTC TTCGACTTCT CAGGGGTCTT CCAAAAGTGT ATTGGGTAGA AGAAAGCACA 
GCTCGGAAAG GCAGAGGTAA CCTCCCAGTT GCAGAGAGCT TCAGCTTCTG CCTGGTGACC 
TTGCTGTCTG ATGTGGCTTG CAGGGACCCC TCACTTAGGG ATGAGATTTT AGAGGCAATT 
TTGCAGGTTT TACATGTCCT TTTGGGAATG TGCCAGGCCT TGGAGATTCA AGAAAAAGAA 
TACCTTTGCA AGTATGCGAT CCCATGCCTG ATAGGAATCT CAAGATCTTT TGGACGTTAC 
AGCAATTCAG AAGAGTCTCT CCTCTCAAAG CTTTTCCCCA AAGTTTCTCC TCATTCCCTG 
AGAATCCCAG AAGAGCTAGA AGGTGTCCGA AGGCGTTCCT TTAATGACTT TCGCTCCATC 
CTGCCCAGCA ACCTGCTAAC TGTCTGTCAG GAGGGCACAC TGAAGAGGAA AACCAGCAGT 
GTGTCCAGCA TCTCTCAGGT TAGCCCAGAA CGTGGCATAC CCCCTCCCAG CTCCCCTGGA 
GGATCTGCAT TTCACTATTT TGAAGCTTCT TGCCTACCTG ATGGGACTGC CTTGGAGCCT 
GAATACTACT TCTCTACCAT CAGCTCCAGT TTTTCTATCT CTCCCCTGTT CAATGGTATT 
ACCTATAAGG AGTTCTGTAT TCCACTGGAA ATGCTTCGGG AACTCTTAAA CTTGGTGAAG 
AAGATTGTTG AGGAGCCTGT TCTCAAATCC TTAGATGCAA TTGTAGCCGG CGTGATGGAG 
GCCAATCCCA GTGCTGATCT GTACTATACA ACCTTCAGTG ATCCCCTGTA CCTGACCATG 
TTCAAGATGC TTCGAGATAC TCTGTACTAC ATGAAAGATC TGCCCACTTC CTTTGTGAAA 
GAGATCCATG ATTTTGTGTT GGAGCAGTTC AACATGAGCC AAGGGGAGCT CCAAAAGATT 
CTGCATGACG CAGATAGGAT CCACAGTGAG ATGAGCCCAC TCAAACTGCG CTGCCAAGCA 
AACGCTGCCT GTGTGGACCT CATGGTGTGG GCTGTGAAGG ATGAGCAGGG TGCAGAAAAC 
CTTTGCATCA AGCTGTCTGA AAAGCTGCAG TCCAAGACAT CCAGTAAGGT CATCATTGCC 
CATTTGCCCT TGCTGATCTG CTGCCTACAG GGTCTGGGCC GCCTGTGTGA AAGGTTCCCA 
GTGGTGGTCC ACTCAGTGAC GCCATCTTTG AGAGACTTTC TGGTAATTCC ATCCCCAGTG 
CTGGTGAAGC TCTATAAGTA CCACAGTCAG TACCACACAG TTGCTGGCAG TGATATAAAA 
ATCAGTGTCA CCAATGAGCA TTCCGAGTCA ACTCTGAATG TCTTGCCGGG CAAGAAGAAC 
CAGCCATCCA TGTATGAGCA GCTCCGAGAC ATCGCCATTG ACAACATCTG CAGGTGCCTG 
AAGGCCGGCC TCACCGTGGA CCCTGTGATC GTGGAGGCCT TCCTGGCCAG CCTCTCCAAC 
CGGCTCTACA TTTCCCAGGA GAGTGACAAG GATGCTCACT TGATTCCTGA CCATACCATC 
CGTGCCTTGG GACACATTGC AGTAGCTTTG AGGGACACTC CAAAGGTCAT GGAGCCAATT 
CTGCAGATCC TGCAGCAGAA ATTTTGCCAG CCCCCCTCAC CCCTTGATGT GCTCATCATC 
GACCAGCTGG GCTGCCTGGT GATCACTGGA AATCAATACA TTTATCAGGA AGTGTGGAAT 
CTCTTCCAGC AAATCAGTGT AAAGGCCAGT TCAGTTGTGT ATTCAGCCAC CAAAGACTAT 
AAAGACCATG GCTACAGGCA CTGCTCCCTA GCAGTGATCA ATGCCCTGGC CAACATTGCA 
GCCAACATTC AGGAGGAGCA TCTTGTGGAC GAGCTGTTGA TGAACCTGCT GGAACTGTTT 
GTGCAGCTGG GGCTGGAGGG GAAAAGAGCC AGTGAGAGGG CCAGTGAAAA GGGGCCTGCC 
CTGAAGGCTT CCAGCAGTGC AGGAAATTTG GGCGTGCTCA TTCCTGTAAT AGCTGTGCTC 
ACCCGACGAT TGCCACCCAT CAAAGAAGCT AAACCTAGGC TACAGAAGCT TTTCCGAGAT 
TTCTGGCTCT ACTCTGTGCT AATGGGATTC GCTGTGGAAG GCTCAGGACT CTGGCCGGAA 
GAGTGGTATG AGGGAGTCTG TGAAATAGCT ACCAAGTCAC CTCTGCTTAC CTTTCCCAGC 
AAAGAGCCTC TGCGATCAGT CCTCCAGTAC AATTCAGCTA TGAAAAACGA TACGGTCACC 
CCTGCTGAAC TGAGTGAGCT CCGAAGTACC ATCATCAACT TGCTAGATCC TCCTCCTGAG 
GTGTCTGCCC TCATCAACAA GCTGGACTTT GCCATGTCTA CCTATCTCTT GTCTGTGTAT 
CGGCTGGAGT ACATGAGGGT ACTGCGTTCT ACAGATCCAG ATCGCTTCCA GGTAATGTTC 
TGCTACTTTG AGGATAAAGC TATTCAAAAA GACAAATCTG GGATGATGCA GTGTGTCATT 
GCTGTTGCTG ACAAAGTGTT TGATGCTTTC CTGAACATGA TGGCAGAAAA AGCTAAGACC 
AAGGAGAATG AAGAGGAGCT GGAGCGGCAT GCACAGTTTC TGCTGGTGAA CTTCAACCAC 
ATTCATAAGA GGATAAGGAG AGTAGCTGAC AAATACCTAT CTGGTCTGGT GGATAAGTTT 
CCACACTTGC TCTGGAGCGG GACTGTGCTG AAGACCATGT TGGACATCCT GCAGACCCTG 
TCACTATCAC TAAGTGCTGA TATTCACAAG GACCAACCTT ACTATGACAT TCCTGATGCC 
CCCTATCGGA TCACGGTTCC TGACACATAC GAAGCCCGGG AGAGCATCGT GAAAGACTTT 
GCTGCGCGCT GTGGAATGAT CCTACAGGAA GCCATGAAGT GGGCACCCAC GGTCACTAAG 
TCCCACCTAC AGGAATATCT GAACAAGCAT CAGAACTGGG TGTCAGGACT GTCTCAGCAC 
ACAGGATTGG CCATGGCCAC TGAGAGCATC CTTCATTTTG CCGGCTACAA CAAGCAGAAC 
ACAACTCTTG GGGCAACTCA GCTAACTGAG CGTCCGGCCT GTGTGAAGAA AGACTACTCC 
AACTTCATGG CATCCTTGAA TTTGCGCAAC CGCTATGCCG GCGAGGTACA TGGAATGATT 
CGGTTCTCAG GTGCCACAGG CCAGATGTCT GACCTGAACA AAATGATGGT CCAAGATCTG 
ATCACAGCTT TGGACCACAG TCATCCTCAG CACTATACAC AAGCCATGTT CAAATTGACG 
GCAATGCTCA TTAGCAGCAA AGATTGTGAT CCACAGCTCC TTCATCATCT GTGTTGGGGT 
CCCCTCCGAA TGTTCAATGA GCATGGCATG GAGACTGCAC TAGCCTGCTG GGAGTGGCTG 
CTGGCTGGCA AGAATGGAGT GGAAGTGCCG TTTATGCGGG AGATGGCAGG AGCCTGGCAC 
ATGACAGTAG AGCAGAAGTT CGGCCTCTTT TCTGTGGAAA CAAAGGAAGC AGACCCCCTG 
GCAGCATCAG AAGCAAGTCA GCCAAGACCT TGTCCCCCCG AAGTGACACC TCACTACATC 
TGGATCGATT TCCTGGTCCA GCGGTTTGAG ATTGCCAAGT ACTGTAGCTC TGACCAGGTG 
GAGATCTTCT CAAGCCTGCT GCAGCGCTCC ATGTCCCTCA ATATTGGTGG AGCCAGGGGG 
AGCATGAACA GGCATGTGGC AGCCATTGGA CCACGTTTCA AACTGCTGAC CTTGGGGCTG 
TCCCTCCTGC ATGCTGATGT GGTTCCAAAT GCAACCATTC GAAACGTGCT TCGAGAGAAG 
ATATACTCAA CAGCCTTTGA CTATTTCAGT TGTCCACCAA AGTTTCCTAC TCAAGGAGAA 
AAGAGGCTTC GTGAAGATAT AAGCATAATG ATTAAGTTTT GGACTGCCAT GTTCTCAGAT 
AAGAAGTACC TGACTGCCAG CCAGCTCGTT CCCCCAGATA ATCAGGACAC CCGAAGCAAC 
TTGGATATAA CTGTGGGCTC TCGACAACAG GCTACCCAGG GCTGGATCAA CACTTACCCC 
CTCTCCAGTG GCATGTCAAC CATCTCCAAA AAGTCAGGTA TGTCTAAGAA AACCAACCGG 
GGCTCCCAGC TGCACAAGTA CTACATGAAA CGCAGGACAC TGCTGCTGTC ACTATTGGCC 
ACTGAGATCG AACGCCTCAT CACTTGGTAC AACCCACTGT CAGCCCCAGA GCTAGAGCTG 
GACCAGGCAG GAGAGAACAG CGTTGCCAAC TGGAGGTCCA AGTACATCAG CTTGAGTGAG 
AAGCAGTGGA AGGACAACGT GAACCTTGCC TGGAGCATCT CTCCATACCT CGCTGTGCAG 
CTGCCAGCCA GGTTCAAGAA TACAGAAGCC ATTGGAAATG AAGTGACCCG TCTCGTTCGC 
TTGGATCCTG GAGCTGTTAG TGATGTCCCT GAAGCCATCA AGTTCCTTGT CACCTGGCAC 
ACCATCGATG CTGATGCTCC AGAGCTCAGC CATGTTTTAT GCTGGGCACC CACGGACCCA 
CCTACAGGCC TCTCCTACTT CTCCAGCATG TACCCACCAC ACCCTCTCAC TGCACAGTAT 
GGGGTAAAAG TCCTGCGATC CTTCCCTCCG GATGCCATTC TGTTCTACAT CCCCCAGATT 
GTGCAGGCCC TCAGATATGA CAAGATGGGC TATGTGCGTG AGTACATTCT CTGGGCTGCA 
GCCAAATCCC AGCTTCTGGC ACACCAGTTT ATTTGGAACA TGAAAACTAA CATCTACCTT 
GATGAAGAAG GGCACCAGAA AGACCCTGAC ATCGGTGACC TCCTGGAGCA GTTAGTAGAA 
GAGATCACAG GGTCTCTATC AGGCCCAGCC AAAGATTTCT ATCAACGGGA ATTTGATTTC 
TTTAACAAGA TCACAAACGT GTCAGCCATC ATCAAGCCCT ACCCTAAAGG CGATGAAAGG 
AAGAAGGCTT GTCTGTCAGC TCTGTCTGAA GTGAAGGTGC AGCCTGGCTG CTACCTGCCC 
AGCAACCCTG AAGCAATCGT GCTAGACATT GACTACAAGT CAGGGACACC AATGCAGAGT 
GCCGCAAAAG CGCCGTATCT GGCCAAGTTT AAGGTGAAGA GATGCGGAGT GAGTGAACTT 
GAAAAAGAAG GTCTGCAGTG CCGCTCAGAC GCAGAGGATG AATGCTTCAG CCAAGAGGCT 
GATGGCAAGA AGATCTGCTG GCAAGCAGCC ATCTTCAAAG TGGGGGATGA CTGCCGACAG 
GACATGTTAG CCCTGCAGAT CATCGACCTC TTCAAGAACA TCTTCCAGCT GGTTGGCCTG 
GACCTCTTCG TTTTTCCTTA TCGGGTAGTG GCTACTGCCC CTGGGTGCGG AGTGATTGAG 
TGCATCCCAG ACTGTACCTC CCGGGACCAG CTGGGCCGCC AGACTGACTT TGGCATGTAT 
GACTACTTTA CACGCCAGTA TGGGGATGAG TCTACCTTGG CCTTCCAGCA GGCACGCTAC 
AACTTTATCA GAAGCATGGC CGCCTATAGC CTCCTGCTGT TCCTGCTGCA GATCAAGGAC 
AGGCACAATG GCAACATCAT GCTGGACAAG AAGGGGCACA TCATCCACAT CGACTTTGGC 
TTCATGTTTG AAAGCTCACC GGGTGGCAAC CTTGGCTGGG AACCAGACAT CAAGCTGACA 
GACGAGATGG TGATGATTAT GGGGGGCAAA ATGGAAGCCA CACCCTTCAA GTGGTTCATG 
GAGATGTGTG TCCGAGGTTA TCTGGCTGTG CGCCCCTATA TGGATGCAGT CGTCTCCCTG 
GTTACACTCA TGTTGGACAC AGGCCTGCCC TGTTTTCGTG GCCAGACAAT CAAACTTTTG 
AAACACAGGT TCAGCCCGAA CATGACAGAG CGGGAGGCTG CGAATTTTAT CATGAAGGTC 
ATCCAGAACT GTTTCCTCAG CAACAGGAGC CGGACCTACG ACATGATCCA GTACTACCAG 
AATGATATCC CCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522097.1
CDS	91..6408
Translation	MAAAGARGGGGGGGGGGGGGSGSSSGSSTSRGFYFNTVLSLARSLAVQRPASLEKVQKLLCMCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDCVVPYLLRLLRGLPKVYWVEESTARKGRGNLPVAESFSFCLVTLLSDVACRDPSLRDEILEAILQVLHVLLGMCQALEIQEKEYLCKYAIPCLIGISRSFGRYSNSEESLLSKLFPKVSPHSLRIPEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPERGIPPPSSPGGSAFHYFEASCLPDGTALEPEYYFSTISSSFSISPLFNGITYKEFCIPLEMLRELLNLVKKIVEEPVLKSLDAIVAGVMEANPSADLYYTTFSDPLYLTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFNMSQGELQKILHDADRIHSEMSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHLPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGSDIKISVTNEHSESTLNVLPGKKNQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQEEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMRVLRSTDPDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQLTERPACVKKDYSNFMASLNLRNRYAGEVHGMIRFSGATGQMSDLNKMMVQDLITALDHSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWHMTVEQKFGLFSVETKEADPLAASEASQPRPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSLNIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAAKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLEQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLQCRSDAEDECFSQEADGKKICWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQNCFLSNRSRTYDMIQYYQNDIPY

Target ORF information:

RefSeq Version	XM_006522034.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol 4-kinase, catalytic, alpha polypeptide (Pi4ka), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522034.1

ORF Insert Sequence:

ATGGCGGCTG CTGGGGCCCG GGGAGGAGGC GGCGGTGGCG GCGGCGGCGG CGGCGGCGGC 
TCGGGGTCCA GCTCTGGCTC CAGCACCTCT CGAGGCTTTT ATTTCAACAC AGTACTTTCA 
CTGGCCCGCT CCCTGGCTGT GCAGAGACCA GCATCCTTGG AGAAGGTTCA AAAGCTGCTT 
TGCATGTGCC CAGTAGATTT TCATGGAATC TTCCAACTAG ATGAAAGACG GAGAGATGCA 
GTGATTGCAT TGGGCATTTT TCTCATTGAA TCTGATCTTC AGCACAAAGA TTGTGTTGTT 
CCTTATCTTC TTCGACTTCT CAGGGGTCTT CCAAAAGTGT ATTGGGTAGA AGAAAGCACA 
GCTCGGAAAG GCAGAGGTAA CCTCCCAGTT GCAGAGAGCT TCAGCTTCTG CCTGGTGACC 
TTGCTGTCTG ATGTGGCTTG CAGGGACCCC TCACTTAGGG ATGAGATTTT AGAGGCAATT 
TTGCAGGTTT TACATGTCCT TTTGGGAATG TGCCAGGCCT TGGAGATTCA AGAAAAAGAA 
TACCTTTGCA AGTATGCGAT CCCATGCCTG ATAGGAATCT CAAGATCTTT TGGACGTTAC 
AGCAATTCAG AAGAGTCTCT CCTCTCAAAG CTTTTCCCCA AAGTTTCTCC TCATTCCCTG 
AGAATCCCAG AAGAGCTAGA AGGTGTCCGA AGGCGTTCCT TTAATGACTT TCGCTCCATC 
CTGCCCAGCA ACCTGCTAAC TGTCTGTCAG GAGGGCACAC TGAAGAGGAA AACCAGCAGT 
GTGTCCAGCA TCTCTCAGGT TAGCCCAGAA CGTGGCATAC CCCCTCCCAG CTCCCCTGGA 
GGATCTGCAT TTCACTATTT TGAAGCTTCT TGCCTACCTG ATGGGACTGC CTTGGAGCCT 
GAATACTACT TCTCTACCAT CAGCTCCAGT TTTTCTATCT CTCCCCTGTT CAATGGTATT 
ACCTATAAGG AGTTCTGTAT TCCACTGGAA ATGCTTCGGG AACTCTTAAA CTTGGTGAAG 
AAGATTGTTG AGGAGCCTGT TCTCAAATCC TTAGATGCAA TTGTAGCCGG CGTGATGGAG 
GCCAATCCCA GTGCTGATCT GTACTATACA ACCTTCAGTG ATCCCCTGTA CCTGACCATG 
TTCAAGATGC TTCGAGATAC TCTGTACTAC ATGAAAGATC TGCCCACTTC CTTTGTGAAA 
GAGATCCATG ATTTTGTGTT GGAGCAGTTC AACATGAGCC AAGGGGAGCT CCAAAAGATT 
CTGCATGACG CAGATAGGAT CCACAGTGAG ATGAGCCCAC TCAAACTGCG CTGCCAAGCA 
AACGCTGCCT GTGTGGACCT CATGGTGTGG GCTGTGAAGG ATGAGCAGGG TGCAGAAAAC 
CTTTGCATCA AGCTGTCTGA AAAGCTGCAG TCCAAGACAT CCAGTAAGGT CATCATTGCC 
CATTTGCCCT TGCTGATCTG CTGCCTACAG GGTCTGGGCC GCCTGTGTGA AAGGTTCCCA 
GTGGTGGTCC ACTCAGTGAC GCCATCTTTG AGAGACTTTC TGGTAATTCC ATCCCCAGTG 
CTGGTGAAGC TCTATAAGTA CCACAGTCAG TACCACACAG TTGCTGGCAG TGATATAAAA 
ATCAGTGTCA CCAATGAGCA TTCCGAGTCA ACTCTGAATG TCTTGCCGGG CAAGAAGAAC 
CAGCCATCCA TGTATGAGCA GCTCCGAGAC ATCGCCATTG ACAACATCTG CAGGTGCCTG 
AAGGCCGGCC TCACCGTGGA CCCTGTGATC GTGGAGGCCT TCCTGGCCAG CCTCTCCAAC 
CGGCTCTACA TTTCCCAGGA GAGTGACAAG GATGCTCACT TGATTCCTGA CCATACCATC 
CGTGCCTTGG GACACATTGC AGTAGCTTTG AGGGACACTC CAAAGGTCAT GGAGCCAATT 
CTGCAGATCC TGCAGCAGAA ATTTTGCCAG CCCCCCTCAC CCCTTGATGT GCTCATCATC 
GACCAGCTGG GCTGCCTGGT GATCACTGGA AATCAATACA TTTATCAGGA AGTGTGGAAT 
CTCTTCCAGC AAATCAGTGT AAAGGCCAGT TCAGTTGTGT ATTCAGCCAC CAAAGACTAT 
AAAGACCATG GCTACAGGCA CTGCTCCCTA GCAGTGATCA ATGCCCTGGC CAACATTGCA 
GCCAACATTC AGGAGGAGCA TCTTGTGGAC GAGCTGTTGA TGAACCTGCT GGAACTGTTT 
GTGCAGCTGG GGCTGGAGGG GAAAAGAGCC AGTGAGAGGG CCAGTGAAAA GGGGCCTGCC 
CTGAAGGCTT CCAGCAGTGC AGGAAATTTG GGCGTGCTCA TTCCTGTAAT AGCTGTGCTC 
ACCCGACGAT TGCCACCCAT CAAAGAAGCT AAACCTAGGC TACAGAAGCT TTTCCGAGAT 
TTCTGGCTCT ACTCTGTGCT AATGGGATTC GCTGTGGAAG GCTCAGGACT CTGGCCGGAA 
GAGTGGTATG AGGGAGTCTG TGAAATAGCT ACCAAGTCAC CTCTGCTTAC CTTTCCCAGC 
AAAGAGCCTC TGCGATCAGT CCTCCAGTAC AATTCAGCTA TGAAAAACGA TACGGTCACC 
CCTGCTGAAC TGAGTGAGCT CCGAAGTACC ATCATCAACT TGCTAGATCC TCCTCCTGAG 
GTGTCTGCCC TCATCAACAA GCTGGACTTT GCCATGTCTA CCTATCTCTT GTCTGTGTAT 
CGGCTGGAGT ACATGAGGGT ACTGCGTTCT ACAGATCCAG ATCGCTTCCA GGTAATGTTC 
TGCTACTTTG AGGATAAAGC TATTCAAAAA GACAAATCTG GGATGATGCA GTGTGTCATT 
GCTGTTGCTG ACAAAGTGTT TGATGCTTTC CTGAACATGA TGGCAGAAAA AGCTAAGACC 
AAGGAGAATG AAGAGGAGCT GGAGCGGCAT GCACAGTTTC TGCTGGTGAA CTTCAACCAC 
ATTCATAAGA GGATAAGGAG AGTAGCTGAC AAATACCTAT CTGGTCTGGT GGATAAGTTT 
CCACACTTGC TCTGGAGCGG GACTGTGCTG AAGACCATGT TGGACATCCT GCAGACCCTG 
TCACTATCAC TAAGTGCTGA TATTCACAAG GACCAACCTT ACTATGACAT TCCTGATGCC 
CCCTATCGGA TCACGGTTCC TGACACATAC GAAGCCCGGG AGAGCATCGT GAAAGACTTT 
GCTGCGCGCT GTGGAATGAT CCTACAGGAA GCCATGAAGT GGGCACCCAC GGTCACTAAG 
TCCCACCTAC AGGAATATCT GAACAAGCAT CAGAACTGGG TGTCAGGACT GTCTCAGCAC 
ACAGGATTGG CCATGGCCAC TGAGAGCATC CTTCATTTTG CCGGCTACAA CAAGCAGAAC 
ACAACTCTTG GGGCAACTCA GCTAACTGAG CGTCCGGCCT GTGTGAAGAA AGACTACTCC 
AACTTCATGG CATCCTTGAA TTTGCGCAAC CGCTATGCCG GCGAGGTACA TGGAATGATT 
CGGTTCTCAG GTGCCACAGG CCAGATGTCT GACCTGAACA AAATGATGGT CCAAGATCTG 
ATCACAGCTT TGGACCACAG TCATCCTCAG CACTATACAC AAGCCATGTT CAAATTGACG 
GCAATGCTCA TTAGCAGCAA AGATTGTGAT CCACAGCTCC TTCATCATCT GTGTTGGGGT 
CCCCTCCGAA TGTTCAATGA GCATGGCATG GAGACTGCAC TAGCCTGCTG GGAGTGGCTG 
CTGGCTGGCA AGAATGGAGT GGAAGTGCCG TTTATGCGGG AGATGGCAGG AGCCTGGCAC 
ATGACAGTAG AGCAGAAGTT CGGCCTCTTT TCTGTGGAAA CAAAGGAAGC AGACCCCCTG 
GCAGCATCAG AAGCAAGTCA GCCAAGACCT TGTCCCCCCG AAGTGACACC TCACTACATC 
TGGATCGATT TCCTGGTCCA GCGGTTTGAG ATTGCCAAGT ACTGTAGCTC TGACCAGGTG 
GAGATCTTCT CAAGCCTGCT GCAGCGCTCC ATGTCCCTCA ATATTGGTGG AGCCAGGGGG 
AGCATGAACA GGCATGTGGC AGCCATTGGA CCACGTTTCA AACTGCTGAC CTTGGGGCTG 
TCCCTCCTGC ATGCTGATGT GGTTCCAAAT GCAACCATTC GAAACGTGCT TCGAGAGAAG 
ATATACTCAA CAGCCTTTGA CTATTTCAGT TGTCCACCAA AGTTTCCTAC TCAAGGAGAA 
AAGAGGCTTC GTGAAGATAT AAGCATAATG ATTAAGTTTT GGACTGCCAT GTTCTCAGAT 
AAGAAGTACC TGACTGCCAG CCAGCTCGTT CCCCCAGATA ATCAGGACAC CCGAAGCAAC 
TTGGATATAA CTGTGGGCTC TCGACAACAG GCTACCCAGG GCTGGATCAA CACTTACCCC 
CTCTCCAGTG GCATGTCAAC CATCTCCAAA AAGTCAGGTA TGTCTAAGAA AACCAACCGG 
GGCTCCCAGC TGCACAAGTA CTACATGAAA CGCAGGACAC TGCTGCTGTC ACTATTGGCC 
ACTGAGATCG AACGCCTCAT CACTTGGTAC AACCCACTGT CAGCCCCAGA GCTAGAGCTG 
GACCAGGCAG GAGAGAACAG CGTTGCCAAC TGGAGGTCCA AGTACATCAG CTTGAGTGAG 
AAGCAGTGGA AGGACAACGT GAACCTTGCC TGGAGCATCT CTCCATACCT CGCTGTGCAG 
CTGCCAGCCA GGTTCAAGAA TACAGAAGCC ATTGGAAATG AAGTGACCCG TCTCGTTCGC 
TTGGATCCTG GAGCTGTTAG TGATGTCCCT GAAGCCATCA AGTTCCTTGT CACCTGGCAC 
ACCATCGATG CTGATGCTCC AGAGCTCAGC CATGTTTTAT GCTGGGCACC CACGGACCCA 
CCTACAGGCC TCTCCTACTT CTCCAGCATG TACCCACCAC ACCCTCTCAC TGCACAGTAT 
GGGGTAAAAG TCCTGCGATC CTTCCCTCCG GATGCCATTC TGTTCTACAT CCCCCAGATT 
GTGCAGGCCC TCAGATATGA CAAGATGGGC TATGTGCGTG AGTACATTCT CTGGGCTGCA 
GCCAAATCCC AGCTTCTGGC ACACCAGTTT ATTTGGAACA TGAAAACTAA CATCTACCTT 
GATGAAGAAG GGCACCAGAA AGACCCTGAC ATCGGTGACC TCCTGGAGCA GTTAGTAGAA 
GAGATCACAG GGTCTCTATC AGGCCCAGCC AAAGATTTCT ATCAACGGGA ATTTGATTTC 
TTTAACAAGA TCACAAACGT GTCAGCCATC ATCAAGCCCT ACCCTAAAGG CGATGAAAGG 
AAGAAGGCTT GTCTGTCAGC TCTGTCTGAA GTGAAGGTGC AGCCTGGCTG CTACCTGCCC 
AGCAACCCTG AAGCAATCGT GCTAGACATT GACTACAAGT CAGGGACACC AATGCAGAGT 
GCCGCAAAAG CGCCGTATCT GGCCAAGTTT AAGGTGAAGA GATGCGGAGT GAGTGAACTT 
GAAAAAGAAG GTCTGCAGTG CCGCTCAGAC GCAGAGGATG AATGCTTCAG CCAAGAGGCT 
GATGGCAAGA AGATCTGCTG GCAAGCAGCC ATCTTCAAAG TGGGGGATGA CTGCCGACAG 
GACATGTTAG CCCTGCAGAT CATCGACCTC TTCAAGAACA TCTTCCAGCT GGTTGGCCTG 
GACCTCTTCG TTTTTCCTTA TCGGGTAGTG GCTACTGCCC CTGGGTGCGG AGTGATTGAG 
TGCATCCCAG ACTGTACCTC CCGGGACCAG CTGGGCCGCC AGACTGACTT TGGCATGTAT 
GACTACTTTA CACGCCAGTA TGGGGATGAG TCTACCTTGG CCTTCCAGCA GGCACGCTAC 
AACTTTATCA GAAGCATGGC CGCCTATAGC CTCCTGCTGT TCCTGCTGCA GATCAAGGAC 
AGGCACAATG GCAACATCAT GCTGGACAAG AAGGGGCACA TCATCCACAT CGACTTTGGC 
TTCATGTTTG AAAGCTCACC GGGTGGCAAC CTTGGCTGGG AACCAGACAT CAAGCTGACA 
GACGAGATGG TGATGATTAT GGGGGGCAAA ATGGAAGCCA CACCCTTCAA GTGGTTCATG 
GAGATGTGTG TCCGAGGTTA TCTGGCTGTG CGCCCCTATA TGGATGCAGT CGTCTCCCTG 
GTTACACTCA TGTTGGACAC AGGCCTGCCC TGTTTTCGTG GCCAGACAAT CAAACTTTTG 
AAACACAGGT TCAGCCCGAA CATGACAGAG CGGGAGGCTG CGAATTTTAT CATGAAGGTC 
ATCCAGAACT GTTTCCTCAG CAACAGGAGC CGGACCTACG ACATGATCCA GTACTACCAG 
AATGATATCC CCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi4ka ( XM_006522036.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522099.1 Mus musculus phosphatidylinositol 4-kinase, catalytic, alpha polypeptide (Pi4ka), transcript variant X2, mRNA.

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CloneID	OMu52053
Clone ID Related Accession (Same CDS sequence)	XM_006522036.3 , XM_006522036.4
Accession Version	XM_006522036.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522036.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CTGGGGCCCG GGGAGGAGGC GGCGGTGGCG GCGGCGGCGG CGGCGGCGGC 
TCGGGGTCCA GCTCTGGCTC CAGCACCTCT CGAGGCTTTT ATTTCAACAC AGTACTTTCA 
CTGGCCCGCT CCCTGGCTGT GCAGAGACCA GCATCCTTGG AGAAGGTTCA AAAGCTGCTT 
TGCATGTGCC CAGTAGATTT TCATGGAATC TTCCAACTAG ATGAAAGACG GAGAGATGCA 
GTGATTGCAT TGGGCATTTT TCTCATTGAA TCTGATCTTC AGCACAAAGA TTGTGTTGTT 
CCTTATCTTC TTCGACTTCT CAGGGGTCTT CCAAAAGTGT ATTGGGTAGA AGAAAGCACA 
GCTCGGAAAG GCAGAGGTAA CCTCCCAGTT GCAGAGAGCT TCAGCTTCTG CCTGGTGACC 
TTGCTGTCTG ATGTGGCTTG CAGGGACCCC TCACTTAGGG ATGAGATTTT AGAGGCAATT 
TTGCAGGTTT TACATGTCCT TTTGGGAATG TGCCAGGCCT TGGAGATTCA AGAAAAAGAA 
TACCTTTGCA AGTATGCGAT CCCATGCCTG ATAGGAATCT CAAGATCTTT TGGACGTTAC 
AGCAATTCAG AAGAGTCTCT CCTCTCAAAG CTTTTCCCCA AAGTTTCTCC TCATTCCCTG 
AGAATCCCAG AAGAGCTAGA AGGTGTCCGA AGGCGTTCCT TTAATGACTT TCGCTCCATC 
CTGCCCAGCA ACCTGCTAAC TGTCTGTCAG GAGGGCACAC TGAAGAGGAA AACCAGCAGT 
GTGTCCAGCA TCTCTCAGGT TAGCCCAGAA CGTGGCATAC CCCCTCCCAG CTCCCCTGGA 
GGATCTGCAT TTCACTATTT TGAAGCTTCT TGCCTACCTG ATGGGACTGC CTTGGAGCCT 
GAATACTACT TCTCTACCAT CAGCTCCAGT TTTTCTATCT CTCCCCTGTT CAATGGTATT 
ACCTATAAGG AGTTCTGTAT TCCACTGGAA ATGCTTCGGG AACTCTTAAA CTTGGTGAAG 
AAGATTGTTG AGGAGCCTGT TCTCAAATCC TTAGATGCAA TTGTAGCCGG CGTGATGGAG 
GCCAATCCCA GTGCTGATCT GTACTATACA ACCTTCAGTG ATCCCCTGTA CCTGACCATG 
TTCAAGATGC TTCGAGATAC TCTGTACTAC ATGAAAGATC TGCCCACTTC CTTTGTGAAA 
GAGATCCATG ATTTTGTGTT GGAGCAGTTC AACATGAGCC AAGGGGAGCT CCAAAAGATT 
CTGCATGACG CAGATAGGAT CCACAGTGAG ATGAGCCCAC TCAAACTGCG CTGCCAAGCA 
AACGCTGCCT GTGTGGACCT CATGGTGTGG GCTGTGAAGG ATGAGCAGGG TGCAGAAAAC 
CTTTGCATCA AGCTGTCTGA AAAGCTGCAG TCCAAGACAT CCAGTAAGGT CATCATTGCC 
CATTTGCCCT TGCTGATCTG CTGCCTACAG GGTCTGGGCC GCCTGTGTGA AAGGTTCCCA 
GTGGTGGTCC ACTCAGTGAC GCCATCTTTG AGAGACTTTC TGGTAATTCC ATCCCCAGTG 
CTGGTGAAGC TCTATAAGTA CCACAGTCAG TACCACACAG TTGCTGGCAG TGATATAAAA 
ATCAGTGTCA CCAATGAGCA TTCCGAGTCA ACTCTGAATG TCTTGCCGGG CAAGAAGAAC 
CAGCCATCCA TGTATGAGCA GCTCCGAGAC ATCGCCATTG ACAACATCTG CAGGTGCCTG 
AAGGCCGGCC TCACCGTGGA CCCTGTGATC GTGGAGGCCT TCCTGGCCAG CCTCTCCAAC 
CGGCTCTACA TTTCCCAGGA GAGTGACAAG GATGCTCACT TGATTCCTGA CCATACCATC 
CGTGCCTTGG GACACATTGC AGTAGCTTTG AGGGACACTC CAAAGGTCAT GGAGCCAATT 
CTGCAGATCC TGCAGCAGAA ATTTTGCCAG CCCCCCTCAC CCCTTGATGT GCTCATCATC 
GACCAGCTGG GCTGCCTGGT GATCACTGGA AATCAATACA TTTATCAGGA AGTGTGGAAT 
CTCTTCCAGC AAATCAGTGT AAAGGCCAGT TCAGTTGTGT ATTCAGCCAC CAAAGACTAT 
AAAGACCATG GCTACAGGCA CTGCTCCCTA GCAGTGATCA ATGCCCTGGC CAACATTGCA 
GCCAACATTC AGGAGGAGCA TCTTGTGGAC GAGCTGTTGA TGAACCTGCT GGAACTGTTT 
GTGCAGCTGG GGCTGGAGGG GAAAAGAGCC AGTGAGAGGG CCAGTGAAAA GGGGCCTGCC 
CTGAAGGCTT CCAGCAGTGC AGGAAATTTG GGCGTGCTCA TTCCTGTAAT AGCTGTGCTC 
ACCCGACGAT TGCCACCCAT CAAAGAAGCT AAACCTAGGC TACAGAAGCT TTTCCGAGAT 
TTCTGGCTCT ACTCTGTGCT AATGGGATTC GCTGTGGAAG GCTCAGGACT CTGGCCGGAA 
GAGTGGTATG AGGGAGTCTG TGAAATAGCT ACCAAGTCAC CTCTGCTTAC CTTTCCCAGC 
AAAGAGCCTC TGCGATCAGT CCTCCAGTAC AATTCAGCTA TGAAAAACGA TACGGTCACC 
CCTGCTGAAC TGAGTGAGCT CCGAAGTACC ATCATCAACT TGCTAGATCC TCCTCCTGAG 
GTGTCTGCCC TCATCAACAA GCTGGACTTT GCCATGTCTA CCTATCTCTT GTCTGTGTAT 
CGGCTGGAGT ACATGAGGGT ACTGCGTTCT ACAGATCCAG ATCGCTTCCA GGTAATGTTC 
TGCTACTTTG AGGATAAAGC TATTCAAAAA GACAAATCTG GGATGATGCA GTGTGTCATT 
GCTGTTGCTG ACAAAGTGTT TGATGCTTTC CTGAACATGA TGGCAGAAAA AGCTAAGACC 
AAGGAGAATG AAGAGGAGCT GGAGCGGCAT GCACAGTTTC TGCTGGTGAA CTTCAACCAC 
ATTCATAAGA GGATAAGGAG AGTAGCTGAC AAATACCTAT CTGGTCTGGT GGATAAGTTT 
CCACACTTGC TCTGGAGCGG GACTGTGCTG AAGACCATGT TGGACATCCT GCAGACCCTG 
TCACTATCAC TAAGTGCTGA TATTCACAAG GACCAACCTT ACTATGACAT TCCTGATGCC 
CCCTATCGGA TCACGGTTCC TGACACATAC GAAGCCCGGG AGAGCATCGT GAAAGACTTT 
GCTGCGCGCT GTGGAATGAT CCTACAGGAA GCCATGAAGT GGGCACCCAC GGTCACTAAG 
TCCCACCTAC AGGAATATCT GAACAAGCAT CAGAACTGGG TGTCAGGACT GTCTCAGCAC 
ACAGGATTGG CCATGGCCAC TGAGAGCATC CTTCATTTTG CCGGCTACAA CAAGCAGAAC 
ACAACTCTTG GGGCAACTCA GCTAACTGAG CGTCCGGCCT GTGTGAAGAA AGACTACTCC 
AACTTCATGG CATCCTTGAA TTTGCGCAAC CGCTATGCCG GCGAGGTACA TGGAATGATT 
CGGTTCTCAG GTGCCACAGG CCAGATGTCT GACCTGAACA AAATGATGGT CCAAGATCTG 
ATCACAGCTT TGGACCACAG TCATCCTCAG CACTATACAC AAGCCATGTT CAAATTGACG 
GCAATGCTCA TTAGCAGCAA AGATTGTGAT CCACAGCTCC TTCATCATCT GTGTTGGGGT 
CCCCTCCGAA TGTTCAATGA GCATGGCATG GAGACTGCAC TAGCCTGCTG GGAGTGGCTG 
CTGGCTGGCA AGAATGGAGT GGAAGTGCCG TTTATGCGGG AGATGGCAGG AGCCTGGCAC 
ATGACAGTAG AGCAGAAGTT CGGCCTCTTT TCTGTGGAAA CAAAGGAAGC AGACCCCCTG 
GCAGCATCAG AAGCAAGTCA GCCAAGACCT TGTCCCCCCG AAGTGACACC TCACTACATC 
TGGATCGATT TCCTGGTCCA GCGGTTTGAG ATTGCCAAGT ACTGTAGCTC TGACCAGGTG 
GAGATCTTCT CAAGCCTGCT GCAGCGCTCC ATGTCCCTCA ATATTGGTGG AGCCAGGGGG 
AGCATGAACA GGCATGTGGC AGCCATTGGA CCACGTTTCA AACTGCTGAC CTTGGGGCTG 
TCCCTCCTGC ATGCTGATGT GGTTCCAAAT GCAACCATTC GAAACGTGCT TCGAGAGAAG 
ATATACTCAA CAGCCTTTGA CTATTTCAGC AGCCATAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522099.1
CDS	91..4272
Translation	MAAAGARGGGGGGGGGGGGGSGSSSGSSTSRGFYFNTVLSLARSLAVQRPASLEKVQKLLCMCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDCVVPYLLRLLRGLPKVYWVEESTARKGRGNLPVAESFSFCLVTLLSDVACRDPSLRDEILEAILQVLHVLLGMCQALEIQEKEYLCKYAIPCLIGISRSFGRYSNSEESLLSKLFPKVSPHSLRIPEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPERGIPPPSSPGGSAFHYFEASCLPDGTALEPEYYFSTISSSFSISPLFNGITYKEFCIPLEMLRELLNLVKKIVEEPVLKSLDAIVAGVMEANPSADLYYTTFSDPLYLTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFNMSQGELQKILHDADRIHSEMSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHLPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGSDIKISVTNEHSESTLNVLPGKKNQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQEEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMRVLRSTDPDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQLTERPACVKKDYSNFMASLNLRNRYAGEVHGMIRFSGATGQMSDLNKMMVQDLITALDHSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWHMTVEQKFGLFSVETKEADPLAASEASQPRPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSLNIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSSHN

Target ORF information:

RefSeq Version	XM_006522036.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol 4-kinase, catalytic, alpha polypeptide (Pi4ka), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522036.3

ORF Insert Sequence:

ATGGCGGCTG CTGGGGCCCG GGGAGGAGGC GGCGGTGGCG GCGGCGGCGG CGGCGGCGGC 
TCGGGGTCCA GCTCTGGCTC CAGCACCTCT CGAGGCTTTT ATTTCAACAC AGTACTTTCA 
CTGGCCCGCT CCCTGGCTGT GCAGAGACCA GCATCCTTGG AGAAGGTTCA AAAGCTGCTT 
TGCATGTGCC CAGTAGATTT TCATGGAATC TTCCAACTAG ATGAAAGACG GAGAGATGCA 
GTGATTGCAT TGGGCATTTT TCTCATTGAA TCTGATCTTC AGCACAAAGA TTGTGTTGTT 
CCTTATCTTC TTCGACTTCT CAGGGGTCTT CCAAAAGTGT ATTGGGTAGA AGAAAGCACA 
GCTCGGAAAG GCAGAGGTAA CCTCCCAGTT GCAGAGAGCT TCAGCTTCTG CCTGGTGACC 
TTGCTGTCTG ATGTGGCTTG CAGGGACCCC TCACTTAGGG ATGAGATTTT AGAGGCAATT 
TTGCAGGTTT TACATGTCCT TTTGGGAATG TGCCAGGCCT TGGAGATTCA AGAAAAAGAA 
TACCTTTGCA AGTATGCGAT CCCATGCCTG ATAGGAATCT CAAGATCTTT TGGACGTTAC 
AGCAATTCAG AAGAGTCTCT CCTCTCAAAG CTTTTCCCCA AAGTTTCTCC TCATTCCCTG 
AGAATCCCAG AAGAGCTAGA AGGTGTCCGA AGGCGTTCCT TTAATGACTT TCGCTCCATC 
CTGCCCAGCA ACCTGCTAAC TGTCTGTCAG GAGGGCACAC TGAAGAGGAA AACCAGCAGT 
GTGTCCAGCA TCTCTCAGGT TAGCCCAGAA CGTGGCATAC CCCCTCCCAG CTCCCCTGGA 
GGATCTGCAT TTCACTATTT TGAAGCTTCT TGCCTACCTG ATGGGACTGC CTTGGAGCCT 
GAATACTACT TCTCTACCAT CAGCTCCAGT TTTTCTATCT CTCCCCTGTT CAATGGTATT 
ACCTATAAGG AGTTCTGTAT TCCACTGGAA ATGCTTCGGG AACTCTTAAA CTTGGTGAAG 
AAGATTGTTG AGGAGCCTGT TCTCAAATCC TTAGATGCAA TTGTAGCCGG CGTGATGGAG 
GCCAATCCCA GTGCTGATCT GTACTATACA ACCTTCAGTG ATCCCCTGTA CCTGACCATG 
TTCAAGATGC TTCGAGATAC TCTGTACTAC ATGAAAGATC TGCCCACTTC CTTTGTGAAA 
GAGATCCATG ATTTTGTGTT GGAGCAGTTC AACATGAGCC AAGGGGAGCT CCAAAAGATT 
CTGCATGACG CAGATAGGAT CCACAGTGAG ATGAGCCCAC TCAAACTGCG CTGCCAAGCA 
AACGCTGCCT GTGTGGACCT CATGGTGTGG GCTGTGAAGG ATGAGCAGGG TGCAGAAAAC 
CTTTGCATCA AGCTGTCTGA AAAGCTGCAG TCCAAGACAT CCAGTAAGGT CATCATTGCC 
CATTTGCCCT TGCTGATCTG CTGCCTACAG GGTCTGGGCC GCCTGTGTGA AAGGTTCCCA 
GTGGTGGTCC ACTCAGTGAC GCCATCTTTG AGAGACTTTC TGGTAATTCC ATCCCCAGTG 
CTGGTGAAGC TCTATAAGTA CCACAGTCAG TACCACACAG TTGCTGGCAG TGATATAAAA 
ATCAGTGTCA CCAATGAGCA TTCCGAGTCA ACTCTGAATG TCTTGCCGGG CAAGAAGAAC 
CAGCCATCCA TGTATGAGCA GCTCCGAGAC ATCGCCATTG ACAACATCTG CAGGTGCCTG 
AAGGCCGGCC TCACCGTGGA CCCTGTGATC GTGGAGGCCT TCCTGGCCAG CCTCTCCAAC 
CGGCTCTACA TTTCCCAGGA GAGTGACAAG GATGCTCACT TGATTCCTGA CCATACCATC 
CGTGCCTTGG GACACATTGC AGTAGCTTTG AGGGACACTC CAAAGGTCAT GGAGCCAATT 
CTGCAGATCC TGCAGCAGAA ATTTTGCCAG CCCCCCTCAC CCCTTGATGT GCTCATCATC 
GACCAGCTGG GCTGCCTGGT GATCACTGGA AATCAATACA TTTATCAGGA AGTGTGGAAT 
CTCTTCCAGC AAATCAGTGT AAAGGCCAGT TCAGTTGTGT ATTCAGCCAC CAAAGACTAT 
AAAGACCATG GCTACAGGCA CTGCTCCCTA GCAGTGATCA ATGCCCTGGC CAACATTGCA 
GCCAACATTC AGGAGGAGCA TCTTGTGGAC GAGCTGTTGA TGAACCTGCT GGAACTGTTT 
GTGCAGCTGG GGCTGGAGGG GAAAAGAGCC AGTGAGAGGG CCAGTGAAAA GGGGCCTGCC 
CTGAAGGCTT CCAGCAGTGC AGGAAATTTG GGCGTGCTCA TTCCTGTAAT AGCTGTGCTC 
ACCCGACGAT TGCCACCCAT CAAAGAAGCT AAACCTAGGC TACAGAAGCT TTTCCGAGAT 
TTCTGGCTCT ACTCTGTGCT AATGGGATTC GCTGTGGAAG GCTCAGGACT CTGGCCGGAA 
GAGTGGTATG AGGGAGTCTG TGAAATAGCT ACCAAGTCAC CTCTGCTTAC CTTTCCCAGC 
AAAGAGCCTC TGCGATCAGT CCTCCAGTAC AATTCAGCTA TGAAAAACGA TACGGTCACC 
CCTGCTGAAC TGAGTGAGCT CCGAAGTACC ATCATCAACT TGCTAGATCC TCCTCCTGAG 
GTGTCTGCCC TCATCAACAA GCTGGACTTT GCCATGTCTA CCTATCTCTT GTCTGTGTAT 
CGGCTGGAGT ACATGAGGGT ACTGCGTTCT ACAGATCCAG ATCGCTTCCA GGTAATGTTC 
TGCTACTTTG AGGATAAAGC TATTCAAAAA GACAAATCTG GGATGATGCA GTGTGTCATT 
GCTGTTGCTG ACAAAGTGTT TGATGCTTTC CTGAACATGA TGGCAGAAAA AGCTAAGACC 
AAGGAGAATG AAGAGGAGCT GGAGCGGCAT GCACAGTTTC TGCTGGTGAA CTTCAACCAC 
ATTCATAAGA GGATAAGGAG AGTAGCTGAC AAATACCTAT CTGGTCTGGT GGATAAGTTT 
CCACACTTGC TCTGGAGCGG GACTGTGCTG AAGACCATGT TGGACATCCT GCAGACCCTG 
TCACTATCAC TAAGTGCTGA TATTCACAAG GACCAACCTT ACTATGACAT TCCTGATGCC 
CCCTATCGGA TCACGGTTCC TGACACATAC GAAGCCCGGG AGAGCATCGT GAAAGACTTT 
GCTGCGCGCT GTGGAATGAT CCTACAGGAA GCCATGAAGT GGGCACCCAC GGTCACTAAG 
TCCCACCTAC AGGAATATCT GAACAAGCAT CAGAACTGGG TGTCAGGACT GTCTCAGCAC 
ACAGGATTGG CCATGGCCAC TGAGAGCATC CTTCATTTTG CCGGCTACAA CAAGCAGAAC 
ACAACTCTTG GGGCAACTCA GCTAACTGAG CGTCCGGCCT GTGTGAAGAA AGACTACTCC 
AACTTCATGG CATCCTTGAA TTTGCGCAAC CGCTATGCCG GCGAGGTACA TGGAATGATT 
CGGTTCTCAG GTGCCACAGG CCAGATGTCT GACCTGAACA AAATGATGGT CCAAGATCTG 
ATCACAGCTT TGGACCACAG TCATCCTCAG CACTATACAC AAGCCATGTT CAAATTGACG 
GCAATGCTCA TTAGCAGCAA AGATTGTGAT CCACAGCTCC TTCATCATCT GTGTTGGGGT 
CCCCTCCGAA TGTTCAATGA GCATGGCATG GAGACTGCAC TAGCCTGCTG GGAGTGGCTG 
CTGGCTGGCA AGAATGGAGT GGAAGTGCCG TTTATGCGGG AGATGGCAGG AGCCTGGCAC 
ATGACAGTAG AGCAGAAGTT CGGCCTCTTT TCTGTGGAAA CAAAGGAAGC AGACCCCCTG 
GCAGCATCAG AAGCAAGTCA GCCAAGACCT TGTCCCCCCG AAGTGACACC TCACTACATC 
TGGATCGATT TCCTGGTCCA GCGGTTTGAG ATTGCCAAGT ACTGTAGCTC TGACCAGGTG 
GAGATCTTCT CAAGCCTGCT GCAGCGCTCC ATGTCCCTCA ATATTGGTGG AGCCAGGGGG 
AGCATGAACA GGCATGTGGC AGCCATTGGA CCACGTTTCA AACTGCTGAC CTTGGGGCTG 
TCCCTCCTGC ATGCTGATGT GGTTCCAAAT GCAACCATTC GAAACGTGCT TCGAGAGAAG 
ATATACTCAA CAGCCTTTGA CTATTTCAGC AGCCATAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread and dynamic translational control of red blood cell development.
Blood129(5)619-629(2017 02)
Alvarez-Dominguez JR,Zhang X,Hu W

Mouse and human CRKL is dosage sensitive for cardiac outflow tract formation.
American journal of human genetics96(2)235-44(2015 Feb)
Racedo SE,McDonald-McGinn DM,Chung JH,Goldmuntz E,Zackai E,Emanuel BS,Zhou B,Funke B,Morrow BE

Selective VPS34 inhibitor blocks autophagy and uncovers a role for NCOA4 in ferritin degradation and iron homeostasis in vivo.
Nature cell biology16(11)1069-79(2014 Nov)
Dowdle WE,Nyfeler B,Nagel J,Elling RA,Liu S,Triantafellow E,Menon S,Wang Z,Honda A,Pardee G,Cantwell J,Luu C,Cornella-Taracido I,Harrington E,Fekkes P,Lei H,Fang Q,Digan ME,Burdick D,Powers AF,Helliwell SB,D'Aquin S,Bastien J,Wang H,Wiederschain D,Kuerth J,Bergman P,Schwalb D,Thomas J,Ugwonali S,Harbinski F,Tallarico J,Wilson CJ,Myer VE,Porter JA,Bussiere DE,Finan PM,Labow MA,Mao X,Hamann LG,Manning BD,Valdez RA,Nicholson T,Schirle M,Knapp MS,Keaney EP,Murphy LO

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Pi4ka cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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