gRNAs
The following Ush2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ush2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu12303 | |
| Clone ID Related Accession (Same CDS sequence) | NM_021408.3 | |
| Accession Version | NM_021408.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 15582bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-20 | |
| Organism | Mus musculus(house mouse) | |
| Product | usherin precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122122.14, AK020845.1, DQ073638.1, AC165230.12 and AC129312.6. On Apr 25, 2007 this sequence version replaced NM_021408.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ073638.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849381, SAMN00849390 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGCATTACC TGGCTCTCTC ACCGGGCTTC CTGTGTTACA CCATTAAAAC ATTGATCCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_067383.3 |
| CDS | 197..15778 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_021408.3 |
1 | ATGCATTACC TGGCTCTCTC ACCGGGCTTC CTGTGTTACA CCATTAAAAC ATTGATCCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu77084 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017319869.1 | |
| Accession Version | XM_017319869.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 9849bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | usherin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END## | |
1 | ATGACGCCAC TATGGAGTTC TGAAGCTCCG CAGAGTGTGC CAACTCCCTC AAGAGCCCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017175358.1 |
| CDS | 1..9849 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017319869.1 |
1 | ATGACGCCAC TATGGAGTTC TGAAGCTCCG CAGAGTGTGC CAACTCCCTC AAGAGCCCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Individual USH2 proteins make distinct contributions to the ankle link complex during development of the mouse cochlear stereociliary bundle. |
Whirlin and PDZ domain-containing 7 (PDZD7) proteins are both required to form the quaternary protein complex associated with Usher syndrome type 2. |
Deletion of PDZD7 disrupts the Usher syndrome type 2 protein complex in cochlear hair cells and causes hearing loss in mice. |
Usher protein functions in hair cells and photoreceptors. |
Elongation factor 1 alpha1 and genes associated with Usher syndromes are downstream targets of GBX2. |
