Usf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usf1
Entrez Gene ID	22278
Full Name	upstream transcription factor 1
Synonyms	bHLHb11
General protein information	Preferred Names upstream transcription factor 1 Names upstream stimulatory factor 1 Upstream stimulatory factor 1 (Major late transcription factor 1) major late transcription factor 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 H3\|1 79.4 cM
Summary	This protein encoded by this gene is a member of the basic-Helix-Hoop-Helix-Leucine zipper (bHLH-LZ) family and encodes a protein that can act as a transcription factor. Studies indicate that the basic region interacts with DNA at E-Box motifs, while the helix-loop-helix and leucine zipper domains are involved in dimerization with different partners. This protein is involved in a wide array of biological pathways, including cell cycle regulation, immune response, and responses to ultraviolet radiation. Mice lacking most of the coding exons of this gene often lacked both whiskers and nasal fur, and were prone to epileptic seizures, while mice lacking both this gene and another family member, Usf2, displayed embryonic lethality (PMID:9520440). Mutations in the human ortholog of this gene have been associated with Familial Combined Hyperlipidemia (FCHL) in humans. Pseudogenes of this gene are found on chromosome 11 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305676.1	NP_001292605.1	upstream stimulatory factor 1 isoform 1
NM_001305677.1	NP_001292606.1	upstream stimulatory factor 1 isoform 1
XM_030253052.1	XP_030108912.1	upstream stimulatory factor 1 isoform X1
NM_009480.3	NP_033506.1	upstream stimulatory factor 1 isoform 1
XM_030253053.1	XP_030108913.1	upstream stimulatory factor 1 isoform X2
XM_030253055.1	XP_030108915.1	upstream stimulatory factor 1 isoform X2
NM_001305678.1	NP_001292607.1	upstream stimulatory factor 1 isoform 2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	usf1	NP_956590.1
Danio rerio (zebrafish)	usf1l	NP_998359.1
Bos taurus (cattle)	USF1	NP_001001161.1
Gallus gallus (chicken)	USF1	NP_001007486.1
Homo sapiens (human)	USF1	NP_009053.1
Mus musculus (house mouse)	Usf1	NP_033506.1
Rattus norvegicus (Norway rat)	Usf1	NP_113965.1
Pan troglodytes (chimpanzee)	USF1	XP_003949666.1
	USF1	XP_001117703.1
Canis lupus familiaris (dog)	USF1	XP_545763.2

Gene Ontology
Bibliography

Related articles in PubMed

Overlapping roles and asymmetrical cross-regulation of the USF proteins in mice.
Sirito M, Lin Q, Deng JM, Behringer RR, Sawadogo M
Proceedings of the National Academy of Sciences of the United States of America95(7)3758-63(1998 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

USF1 exclusively bound to the CACGTG E-box motifs in the brain cortex neuron proximal promoter regions. Importantly, functional annotation of the USF1-binding targets revealed an enrichment of genes related to lysosomal functions.
Title: Genome-wide analyses in neuronal cells reveal that upstream transcription factors regulate lysosomal gene expression.

Mice lacking Usf1 displayed increased BAT-facilitated, diet-induced thermogenesis with up-regulation of mitochondrial respiratory chain complexes, as well as increased BAT activity even at thermoneutrality and after BAT sympathectomy
Title: USF1 deficiency activates brown adipose tissue and improves cardiometabolic health.

USF1 but not USF2 supports OPN expression in LRP6-VKO vascular smooth muscle lineage, and immunoprecipitation confirmed increased USF1 association with OPN chromatin.
Title: Vascular smooth muscle LRP6 limits arteriosclerotic calcification in diabetic LDLR-/- mice by restraining noncanonical Wnt signals.

Pdx1, USF1 and USF2 co-ordinately regulate Alx3 gene expression in pancreatic beta-cells.
Title: Pdx1 and USF transcription factors co-ordinately regulate Alx3 gene expression in pancreatic ?-cells.

They underscore the new role of USF1 and give new clues of how p53 loss of function can occur in any cell type.
Title: p53 requires the stress sensor USF1 to direct appropriate cell fate decision.

The following Usf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22889	NM_001305677.1 Latest version!	Mus musculus upstream transcription factor 1 (Usf1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu22889	NM_001305676.1 Latest version!	Mus musculus upstream transcription factor 1 (Usf1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu22889	NM_009480.3 Latest version!	Mus musculus upstream transcription factor 1 (Usf1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu69022	NM_001305678.1 Latest version!	Mus musculus upstream transcription factor 1 (Usf1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu22889	XM_030253052.1 Latest version!	Mus musculus upstream transcription factor 1 (Usf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu69022	XM_030253053.1 Latest version!	Mus musculus upstream transcription factor 1 (Usf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu69022	XM_030253055.1 Latest version!	Mus musculus upstream transcription factor 1 (Usf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usf1 ( NM_001305676.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001292605.1 Mus musculus upstream transcription factor 1 (Usf1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22889
Clone ID Related Accession (Same CDS sequence)	NM_001305676.1 , NM_001305677.1 , XM_030253052.1 , NM_009480.3
Accession Version	NM_001305676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	upstream stimulatory factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ050979.1, AK142437.1, AW555090.2 and BE995823.1. On Mar 30, 2015 this sequence version replaced XM_006496747.2. Transcript Variant: This variant (2) uses an alternate splice site in its 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK142437.1, SRR1660811.154340.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGGGC AGCAGAAAAC AGCTGAAACC GAAGAGGGAA CAGTGCAGAT TCAGGAAGGC 
GCAGTGGCTA CTGGAGAGGA CCCAACTAGT GTAGCTATCG CCAGCATCCA GTCAGCTGCC 
ACTTTTCCTG ACCCCAACGT CAAGTACGTC TTCCGAACTG AGAATGGGGG CCAGGTGATG 
TACAGGGTGA TCCAGGTGTC AGAGGGGCAG CTGGATGGCC AGACAGAGGG CTCTGGCGCC 
ATCAGTGGTT ACCCTGCCAC TCAGTCTATG ACCCAGGCAG TGATCCAGGG AGCTTTCACC 
AGTGACGATG CCGTTGACAC GGAGGGAGCA GCTGCTGAGA CACATTATAC ATATTTCCCC 
AGCACCGCAG TGGGAGATGG GTCAGGGGGT ACCACATCTG GGAGTACTAC AGCTGTTGTT 
ACCACCCAGG GCTCAGAGGC ACTACTGGGG CAGGCAACCC CGCCCAGCAC AGGTCAATTC 
TTTGTGATGA TGTCACCACA AGAAGTATTG CAGGGAGGGA GCCAGCGATC GATTGCCCCC 
AGGACCCACC CTTATTCCCC GAAGTCAGAG GCTCCCAGGA CAACTCGAGA TGAGAAACGG 
AGGGCTCAAC ATAACGAAGT GGAGCGCCGC CGCCGGGACA AGATCAACAA CTGGATTGTA 
CAGCTGTCCA AAATCATCCC AGACTGCTCT ATGGAGAGCA CCAAGTCTGG CCAGAGTAAA 
GGTGGAATCC TGTCCAAAGC CTGTGATTAT ATCCAGGAGC TGCGGCAGAG CAACCACCGG 
CTGTCTGAAG AGCTGCAGGG GTTAGATCAG TTGCAGCTGG ACAACGATGT GCTCCGGCAA 
CAGGTGGAAG ATCTCAAGAA CAAGAACCTG CTCCTGCGAG CTCAGCTTCG GCACCACGGA 
CTCGAGGTCG TCATCAAGAA TGACAGCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292605.1
CDS	214..1146
Translation	MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGSGAISGYPATQSMTQAVIQGAFTSDDAVDTEGAAAETHYTYFPSTAVGDGSGGTTSGSTTAVVTTQGSEALLGQATPPSTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

Target ORF information:

RefSeq Version	NM_001305676.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus upstream transcription factor 1 (Usf1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305676.1

ORF Insert Sequence:

ATGAAGGGGC AGCAGAAAAC AGCTGAAACC GAAGAGGGAA CAGTGCAGAT TCAGGAAGGC 
GCAGTGGCTA CTGGAGAGGA CCCAACTAGT GTAGCTATCG CCAGCATCCA GTCAGCTGCC 
ACTTTTCCTG ACCCCAACGT CAAGTACGTC TTCCGAACTG AGAATGGGGG CCAGGTGATG 
TACAGGGTGA TCCAGGTGTC AGAGGGGCAG CTGGATGGCC AGACAGAGGG CTCTGGCGCC 
ATCAGTGGTT ACCCTGCCAC TCAGTCTATG ACCCAGGCAG TGATCCAGGG AGCTTTCACC 
AGTGACGATG CCGTTGACAC GGAGGGAGCA GCTGCTGAGA CACATTATAC ATATTTCCCC 
AGCACCGCAG TGGGAGATGG GTCAGGGGGT ACCACATCTG GGAGTACTAC AGCTGTTGTT 
ACCACCCAGG GCTCAGAGGC ACTACTGGGG CAGGCAACCC CGCCCAGCAC AGGTCAATTC 
TTTGTGATGA TGTCACCACA AGAAGTATTG CAGGGAGGGA GCCAGCGATC GATTGCCCCC 
AGGACCCACC CTTATTCCCC GAAGTCAGAG GCTCCCAGGA CAACTCGAGA TGAGAAACGG 
AGGGCTCAAC ATAACGAAGT GGAGCGCCGC CGCCGGGACA AGATCAACAA CTGGATTGTA 
CAGCTGTCCA AAATCATCCC AGACTGCTCT ATGGAGAGCA CCAAGTCTGG CCAGAGTAAA 
GGTGGAATCC TGTCCAAAGC CTGTGATTAT ATCCAGGAGC TGCGGCAGAG CAACCACCGG 
CTGTCTGAAG AGCTGCAGGG GTTAGATCAG TTGCAGCTGG ACAACGATGT GCTCCGGCAA 
CAGGTGGAAG ATCTCAAGAA CAAGAACCTG CTCCTGCGAG CTCAGCTTCG GCACCACGGA 
CTCGAGGTCG TCATCAAGAA TGACAGCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usf1 ( NM_001305677.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001292606.1 Mus musculus upstream transcription factor 1 (Usf1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22889
Clone ID Related Accession (Same CDS sequence)	NM_001305676.1 , NM_001305677.1 , XM_030253052.1 , NM_009480.3
Accession Version	NM_001305677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	upstream stimulatory factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ050979.1, AK142437.1, AW555090.2 and BE995823.1. On Mar 30, 2015 this sequence version replaced XM_006496748.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1, 2, and 3 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK142437.1, SRR1660811.154340.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGGGGC AGCAGAAAAC AGCTGAAACC GAAGAGGGAA CAGTGCAGAT TCAGGAAGGC 
GCAGTGGCTA CTGGAGAGGA CCCAACTAGT GTAGCTATCG CCAGCATCCA GTCAGCTGCC 
ACTTTTCCTG ACCCCAACGT CAAGTACGTC TTCCGAACTG AGAATGGGGG CCAGGTGATG 
TACAGGGTGA TCCAGGTGTC AGAGGGGCAG CTGGATGGCC AGACAGAGGG CTCTGGCGCC 
ATCAGTGGTT ACCCTGCCAC TCAGTCTATG ACCCAGGCAG TGATCCAGGG AGCTTTCACC 
AGTGACGATG CCGTTGACAC GGAGGGAGCA GCTGCTGAGA CACATTATAC ATATTTCCCC 
AGCACCGCAG TGGGAGATGG GTCAGGGGGT ACCACATCTG GGAGTACTAC AGCTGTTGTT 
ACCACCCAGG GCTCAGAGGC ACTACTGGGG CAGGCAACCC CGCCCAGCAC AGGTCAATTC 
TTTGTGATGA TGTCACCACA AGAAGTATTG CAGGGAGGGA GCCAGCGATC GATTGCCCCC 
AGGACCCACC CTTATTCCCC GAAGTCAGAG GCTCCCAGGA CAACTCGAGA TGAGAAACGG 
AGGGCTCAAC ATAACGAAGT GGAGCGCCGC CGCCGGGACA AGATCAACAA CTGGATTGTA 
CAGCTGTCCA AAATCATCCC AGACTGCTCT ATGGAGAGCA CCAAGTCTGG CCAGAGTAAA 
GGTGGAATCC TGTCCAAAGC CTGTGATTAT ATCCAGGAGC TGCGGCAGAG CAACCACCGG 
CTGTCTGAAG AGCTGCAGGG GTTAGATCAG TTGCAGCTGG ACAACGATGT GCTCCGGCAA 
CAGGTGGAAG ATCTCAAGAA CAAGAACCTG CTCCTGCGAG CTCAGCTTCG GCACCACGGA 
CTCGAGGTCG TCATCAAGAA TGACAGCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292606.1
CDS	278..1210
Translation	MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGSGAISGYPATQSMTQAVIQGAFTSDDAVDTEGAAAETHYTYFPSTAVGDGSGGTTSGSTTAVVTTQGSEALLGQATPPSTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

Target ORF information:

RefSeq Version	NM_001305677.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus upstream transcription factor 1 (Usf1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305677.1

ORF Insert Sequence:

ATGAAGGGGC AGCAGAAAAC AGCTGAAACC GAAGAGGGAA CAGTGCAGAT TCAGGAAGGC 
GCAGTGGCTA CTGGAGAGGA CCCAACTAGT GTAGCTATCG CCAGCATCCA GTCAGCTGCC 
ACTTTTCCTG ACCCCAACGT CAAGTACGTC TTCCGAACTG AGAATGGGGG CCAGGTGATG 
TACAGGGTGA TCCAGGTGTC AGAGGGGCAG CTGGATGGCC AGACAGAGGG CTCTGGCGCC 
ATCAGTGGTT ACCCTGCCAC TCAGTCTATG ACCCAGGCAG TGATCCAGGG AGCTTTCACC 
AGTGACGATG CCGTTGACAC GGAGGGAGCA GCTGCTGAGA CACATTATAC ATATTTCCCC 
AGCACCGCAG TGGGAGATGG GTCAGGGGGT ACCACATCTG GGAGTACTAC AGCTGTTGTT 
ACCACCCAGG GCTCAGAGGC ACTACTGGGG CAGGCAACCC CGCCCAGCAC AGGTCAATTC 
TTTGTGATGA TGTCACCACA AGAAGTATTG CAGGGAGGGA GCCAGCGATC GATTGCCCCC 
AGGACCCACC CTTATTCCCC GAAGTCAGAG GCTCCCAGGA CAACTCGAGA TGAGAAACGG 
AGGGCTCAAC ATAACGAAGT GGAGCGCCGC CGCCGGGACA AGATCAACAA CTGGATTGTA 
CAGCTGTCCA AAATCATCCC AGACTGCTCT ATGGAGAGCA CCAAGTCTGG CCAGAGTAAA 
GGTGGAATCC TGTCCAAAGC CTGTGATTAT ATCCAGGAGC TGCGGCAGAG CAACCACCGG 
CTGTCTGAAG AGCTGCAGGG GTTAGATCAG TTGCAGCTGG ACAACGATGT GCTCCGGCAA 
CAGGTGGAAG ATCTCAAGAA CAAGAACCTG CTCCTGCGAG CTCAGCTTCG GCACCACGGA 
CTCGAGGTCG TCATCAAGAA TGACAGCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usf1 ( NM_009480.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033506.1 Mus musculus upstream transcription factor 1 (Usf1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu22889
Clone ID Related Accession (Same CDS sequence)	NM_001305676.1 , NM_001305677.1 , XM_030253052.1 , NM_009480.3
Accession Version	NM_009480.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	upstream stimulatory factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087229.27, AK142437.1, AW555090.2 and BE995823.1. On Mar 30, 2015 this sequence version replaced NM_009480.2. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. The 5' terminal exon of this transcript was described in PMID:8938446, and is supported by RNASeq data. Variants 1, 2, and 3 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the 5' terminal exon were described in PMID: 8938446. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK142437.1, SRR1660811.154340.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 8938446 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGGGGC AGCAGAAAAC AGCTGAAACC GAAGAGGGAA CAGTGCAGAT TCAGGAAGGC 
GCAGTGGCTA CTGGAGAGGA CCCAACTAGT GTAGCTATCG CCAGCATCCA GTCAGCTGCC 
ACTTTTCCTG ACCCCAACGT CAAGTACGTC TTCCGAACTG AGAATGGGGG CCAGGTGATG 
TACAGGGTGA TCCAGGTGTC AGAGGGGCAG CTGGATGGCC AGACAGAGGG CTCTGGCGCC 
ATCAGTGGTT ACCCTGCCAC TCAGTCTATG ACCCAGGCAG TGATCCAGGG AGCTTTCACC 
AGTGACGATG CCGTTGACAC GGAGGGAGCA GCTGCTGAGA CACATTATAC ATATTTCCCC 
AGCACCGCAG TGGGAGATGG GTCAGGGGGT ACCACATCTG GGAGTACTAC AGCTGTTGTT 
ACCACCCAGG GCTCAGAGGC ACTACTGGGG CAGGCAACCC CGCCCAGCAC AGGTCAATTC 
TTTGTGATGA TGTCACCACA AGAAGTATTG CAGGGAGGGA GCCAGCGATC GATTGCCCCC 
AGGACCCACC CTTATTCCCC GAAGTCAGAG GCTCCCAGGA CAACTCGAGA TGAGAAACGG 
AGGGCTCAAC ATAACGAAGT GGAGCGCCGC CGCCGGGACA AGATCAACAA CTGGATTGTA 
CAGCTGTCCA AAATCATCCC AGACTGCTCT ATGGAGAGCA CCAAGTCTGG CCAGAGTAAA 
GGTGGAATCC TGTCCAAAGC CTGTGATTAT ATCCAGGAGC TGCGGCAGAG CAACCACCGG 
CTGTCTGAAG AGCTGCAGGG GTTAGATCAG TTGCAGCTGG ACAACGATGT GCTCCGGCAA 
CAGGTGGAAG ATCTCAAGAA CAAGAACCTG CTCCTGCGAG CTCAGCTTCG GCACCACGGA 
CTCGAGGTCG TCATCAAGAA TGACAGCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033506.1
CDS	268..1200
Translation	MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGSGAISGYPATQSMTQAVIQGAFTSDDAVDTEGAAAETHYTYFPSTAVGDGSGGTTSGSTTAVVTTQGSEALLGQATPPSTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

Target ORF information:

RefSeq Version	NM_009480.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus upstream transcription factor 1 (Usf1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009480.3

ORF Insert Sequence:

ATGAAGGGGC AGCAGAAAAC AGCTGAAACC GAAGAGGGAA CAGTGCAGAT TCAGGAAGGC 
GCAGTGGCTA CTGGAGAGGA CCCAACTAGT GTAGCTATCG CCAGCATCCA GTCAGCTGCC 
ACTTTTCCTG ACCCCAACGT CAAGTACGTC TTCCGAACTG AGAATGGGGG CCAGGTGATG 
TACAGGGTGA TCCAGGTGTC AGAGGGGCAG CTGGATGGCC AGACAGAGGG CTCTGGCGCC 
ATCAGTGGTT ACCCTGCCAC TCAGTCTATG ACCCAGGCAG TGATCCAGGG AGCTTTCACC 
AGTGACGATG CCGTTGACAC GGAGGGAGCA GCTGCTGAGA CACATTATAC ATATTTCCCC 
AGCACCGCAG TGGGAGATGG GTCAGGGGGT ACCACATCTG GGAGTACTAC AGCTGTTGTT 
ACCACCCAGG GCTCAGAGGC ACTACTGGGG CAGGCAACCC CGCCCAGCAC AGGTCAATTC 
TTTGTGATGA TGTCACCACA AGAAGTATTG CAGGGAGGGA GCCAGCGATC GATTGCCCCC 
AGGACCCACC CTTATTCCCC GAAGTCAGAG GCTCCCAGGA CAACTCGAGA TGAGAAACGG 
AGGGCTCAAC ATAACGAAGT GGAGCGCCGC CGCCGGGACA AGATCAACAA CTGGATTGTA 
CAGCTGTCCA AAATCATCCC AGACTGCTCT ATGGAGAGCA CCAAGTCTGG CCAGAGTAAA 
GGTGGAATCC TGTCCAAAGC CTGTGATTAT ATCCAGGAGC TGCGGCAGAG CAACCACCGG 
CTGTCTGAAG AGCTGCAGGG GTTAGATCAG TTGCAGCTGG ACAACGATGT GCTCCGGCAA 
CAGGTGGAAG ATCTCAAGAA CAAGAACCTG CTCCTGCGAG CTCAGCTTCG GCACCACGGA 
CTCGAGGTCG TCATCAAGAA TGACAGCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usf1 ( NM_001305678.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001292607.1 Mus musculus upstream transcription factor 1 (Usf1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu69022
Clone ID Related Accession (Same CDS sequence)	XM_030253053.1 , XM_030253055.1 , NM_001305678.1
Accession Version	NM_001305678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	upstream stimulatory factor 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ050979.1, DV653810.1, AK142437.1, AW555090.2 and BE995823.1. On Mar 30, 2015 this sequence version replaced XM_006496749.2. Transcript Variant: This variant (4) uses an alternate splice site at an internal exon compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG to encode an isoform (2) that has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1660817.322853.1, SRR1660819.319355.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACAGGG TGATCCAGGT GTCAGAGGGG CAGCTGGATG GCCAGACAGA GGGCTCTGGC 
GCCATCAGTG GTTACCCTGC CACTCAGTCT ATGACCCAGG CAGTGATCCA GGGAGCTTTC 
ACCAGTGACG ATGCCGTTGA CACGGAGGGA GCAGCTGCTG AGACACATTA TACATATTTC 
CCCAGCACCG CAGTGGGAGA TGGGTCAGGG GGTACCACAT CTGGGAGTAC TACAGCTGTT 
GTTACCACCC AGGGCTCAGA GGCACTACTG GGGCAGGCAA CCCCGCCCAG CACAGGTCAA 
TTCTTTGTGA TGATGTCACC ACAAGAAGTA TTGCAGGGAG GGAGCCAGCG ATCGATTGCC 
CCCAGGACCC ACCCTTATTC CCCGAAGTCA GAGGCTCCCA GGACAACTCG AGATGAGAAA 
CGGAGGGCTC AACATAACGA AGTGGAGCGC CGCCGCCGGG ACAAGATCAA CAACTGGATT 
GTACAGCTGT CCAAAATCAT CCCAGACTGC TCTATGGAGA GCACCAAGTC TGGCCAGAGT 
AAAGGTGGAA TCCTGTCCAA AGCCTGTGAT TATATCCAGG AGCTGCGGCA GAGCAACCAC 
CGGCTGTCTG AAGAGCTGCA GGGGTTAGAT CAGTTGCAGC TGGACAACGA TGTGCTCCGG 
CAACAGGTGG AAGATCTCAA GAACAAGAAC CTGCTCCTGC GAGCTCAGCT TCGGCACCAC 
GGACTCGAGG TCGTCATCAA GAATGACAGC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292607.1
CDS	360..1115
Translation	MYRVIQVSEGQLDGQTEGSGAISGYPATQSMTQAVIQGAFTSDDAVDTEGAAAETHYTYFPSTAVGDGSGGTTSGSTTAVVTTQGSEALLGQATPPSTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

Target ORF information:

RefSeq Version	NM_001305678.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus upstream transcription factor 1 (Usf1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305678.1

ORF Insert Sequence:

ATGTACAGGG TGATCCAGGT GTCAGAGGGG CAGCTGGATG GCCAGACAGA GGGCTCTGGC 
GCCATCAGTG GTTACCCTGC CACTCAGTCT ATGACCCAGG CAGTGATCCA GGGAGCTTTC 
ACCAGTGACG ATGCCGTTGA CACGGAGGGA GCAGCTGCTG AGACACATTA TACATATTTC 
CCCAGCACCG CAGTGGGAGA TGGGTCAGGG GGTACCACAT CTGGGAGTAC TACAGCTGTT 
GTTACCACCC AGGGCTCAGA GGCACTACTG GGGCAGGCAA CCCCGCCCAG CACAGGTCAA 
TTCTTTGTGA TGATGTCACC ACAAGAAGTA TTGCAGGGAG GGAGCCAGCG ATCGATTGCC 
CCCAGGACCC ACCCTTATTC CCCGAAGTCA GAGGCTCCCA GGACAACTCG AGATGAGAAA 
CGGAGGGCTC AACATAACGA AGTGGAGCGC CGCCGCCGGG ACAAGATCAA CAACTGGATT 
GTACAGCTGT CCAAAATCAT CCCAGACTGC TCTATGGAGA GCACCAAGTC TGGCCAGAGT 
AAAGGTGGAA TCCTGTCCAA AGCCTGTGAT TATATCCAGG AGCTGCGGCA GAGCAACCAC 
CGGCTGTCTG AAGAGCTGCA GGGGTTAGAT CAGTTGCAGC TGGACAACGA TGTGCTCCGG 
CAACAGGTGG AAGATCTCAA GAACAAGAAC CTGCTCCTGC GAGCTCAGCT TCGGCACCAC 
GGACTCGAGG TCGTCATCAA GAATGACAGC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usf1 ( XM_030253052.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108912.1 Mus musculus upstream transcription factor 1 (Usf1), transcript variant X1, mRNA.

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CloneID	OMu22889
Clone ID Related Accession (Same CDS sequence)	NM_001305676.1 , NM_001305677.1 , XM_030253052.1 , NM_009480.3
Accession Version	XM_030253052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	upstream stimulatory factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGGC AGCAGAAAAC AGCTGAAACC GAAGAGGGAA CAGTGCAGAT TCAGGAAGGC 
GCAGTGGCTA CTGGAGAGGA CCCAACTAGT GTAGCTATCG CCAGCATCCA GTCAGCTGCC 
ACTTTTCCTG ACCCCAACGT CAAGTACGTC TTCCGAACTG AGAATGGGGG CCAGGTGATG 
TACAGGGTGA TCCAGGTGTC AGAGGGGCAG CTGGATGGCC AGACAGAGGG CTCTGGCGCC 
ATCAGTGGTT ACCCTGCCAC TCAGTCTATG ACCCAGGCAG TGATCCAGGG AGCTTTCACC 
AGTGACGATG CCGTTGACAC GGAGGGAGCA GCTGCTGAGA CACATTATAC ATATTTCCCC 
AGCACCGCAG TGGGAGATGG GTCAGGGGGT ACCACATCTG GGAGTACTAC AGCTGTTGTT 
ACCACCCAGG GCTCAGAGGC ACTACTGGGG CAGGCAACCC CGCCCAGCAC AGGTCAATTC 
TTTGTGATGA TGTCACCACA AGAAGTATTG CAGGGAGGGA GCCAGCGATC GATTGCCCCC 
AGGACCCACC CTTATTCCCC GAAGTCAGAG GCTCCCAGGA CAACTCGAGA TGAGAAACGG 
AGGGCTCAAC ATAACGAAGT GGAGCGCCGC CGCCGGGACA AGATCAACAA CTGGATTGTA 
CAGCTGTCCA AAATCATCCC AGACTGCTCT ATGGAGAGCA CCAAGTCTGG CCAGAGTAAA 
GGTGGAATCC TGTCCAAAGC CTGTGATTAT ATCCAGGAGC TGCGGCAGAG CAACCACCGG 
CTGTCTGAAG AGCTGCAGGG GTTAGATCAG TTGCAGCTGG ACAACGATGT GCTCCGGCAA 
CAGGTGGAAG ATCTCAAGAA CAAGAACCTG CTCCTGCGAG CTCAGCTTCG GCACCACGGA 
CTCGAGGTCG TCATCAAGAA TGACAGCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108912.1
CDS	292..1224
Translation	MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGSGAISGYPATQSMTQAVIQGAFTSDDAVDTEGAAAETHYTYFPSTAVGDGSGGTTSGSTTAVVTTQGSEALLGQATPPSTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

Target ORF information:

RefSeq Version	XM_030253052.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus upstream transcription factor 1 (Usf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253052.1

ORF Insert Sequence:

ATGAAGGGGC AGCAGAAAAC AGCTGAAACC GAAGAGGGAA CAGTGCAGAT TCAGGAAGGC 
GCAGTGGCTA CTGGAGAGGA CCCAACTAGT GTAGCTATCG CCAGCATCCA GTCAGCTGCC 
ACTTTTCCTG ACCCCAACGT CAAGTACGTC TTCCGAACTG AGAATGGGGG CCAGGTGATG 
TACAGGGTGA TCCAGGTGTC AGAGGGGCAG CTGGATGGCC AGACAGAGGG CTCTGGCGCC 
ATCAGTGGTT ACCCTGCCAC TCAGTCTATG ACCCAGGCAG TGATCCAGGG AGCTTTCACC 
AGTGACGATG CCGTTGACAC GGAGGGAGCA GCTGCTGAGA CACATTATAC ATATTTCCCC 
AGCACCGCAG TGGGAGATGG GTCAGGGGGT ACCACATCTG GGAGTACTAC AGCTGTTGTT 
ACCACCCAGG GCTCAGAGGC ACTACTGGGG CAGGCAACCC CGCCCAGCAC AGGTCAATTC 
TTTGTGATGA TGTCACCACA AGAAGTATTG CAGGGAGGGA GCCAGCGATC GATTGCCCCC 
AGGACCCACC CTTATTCCCC GAAGTCAGAG GCTCCCAGGA CAACTCGAGA TGAGAAACGG 
AGGGCTCAAC ATAACGAAGT GGAGCGCCGC CGCCGGGACA AGATCAACAA CTGGATTGTA 
CAGCTGTCCA AAATCATCCC AGACTGCTCT ATGGAGAGCA CCAAGTCTGG CCAGAGTAAA 
GGTGGAATCC TGTCCAAAGC CTGTGATTAT ATCCAGGAGC TGCGGCAGAG CAACCACCGG 
CTGTCTGAAG AGCTGCAGGG GTTAGATCAG TTGCAGCTGG ACAACGATGT GCTCCGGCAA 
CAGGTGGAAG ATCTCAAGAA CAAGAACCTG CTCCTGCGAG CTCAGCTTCG GCACCACGGA 
CTCGAGGTCG TCATCAAGAA TGACAGCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usf1 ( XM_030253053.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108913.1 Mus musculus upstream transcription factor 1 (Usf1), transcript variant X2, mRNA.

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CloneID	OMu69022
Clone ID Related Accession (Same CDS sequence)	XM_030253053.1 , XM_030253055.1 , NM_001305678.1
Accession Version	XM_030253053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	upstream stimulatory factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGGG TGATCCAGGT GTCAGAGGGG CAGCTGGATG GCCAGACAGA GGGCTCTGGC 
GCCATCAGTG GTTACCCTGC CACTCAGTCT ATGACCCAGG CAGTGATCCA GGGAGCTTTC 
ACCAGTGACG ATGCCGTTGA CACGGAGGGA GCAGCTGCTG AGACACATTA TACATATTTC 
CCCAGCACCG CAGTGGGAGA TGGGTCAGGG GGTACCACAT CTGGGAGTAC TACAGCTGTT 
GTTACCACCC AGGGCTCAGA GGCACTACTG GGGCAGGCAA CCCCGCCCAG CACAGGTCAA 
TTCTTTGTGA TGATGTCACC ACAAGAAGTA TTGCAGGGAG GGAGCCAGCG ATCGATTGCC 
CCCAGGACCC ACCCTTATTC CCCGAAGTCA GAGGCTCCCA GGACAACTCG AGATGAGAAA 
CGGAGGGCTC AACATAACGA AGTGGAGCGC CGCCGCCGGG ACAAGATCAA CAACTGGATT 
GTACAGCTGT CCAAAATCAT CCCAGACTGC TCTATGGAGA GCACCAAGTC TGGCCAGAGT 
AAAGGTGGAA TCCTGTCCAA AGCCTGTGAT TATATCCAGG AGCTGCGGCA GAGCAACCAC 
CGGCTGTCTG AAGAGCTGCA GGGGTTAGAT CAGTTGCAGC TGGACAACGA TGTGCTCCGG 
CAACAGGTGG AAGATCTCAA GAACAAGAAC CTGCTCCTGC GAGCTCAGCT TCGGCACCAC 
GGACTCGAGG TCGTCATCAA GAATGACAGC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108913.1
CDS	430..1185
Translation	MYRVIQVSEGQLDGQTEGSGAISGYPATQSMTQAVIQGAFTSDDAVDTEGAAAETHYTYFPSTAVGDGSGGTTSGSTTAVVTTQGSEALLGQATPPSTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

Target ORF information:

RefSeq Version	XM_030253053.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus upstream transcription factor 1 (Usf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253053.1

ORF Insert Sequence:

ATGTACAGGG TGATCCAGGT GTCAGAGGGG CAGCTGGATG GCCAGACAGA GGGCTCTGGC 
GCCATCAGTG GTTACCCTGC CACTCAGTCT ATGACCCAGG CAGTGATCCA GGGAGCTTTC 
ACCAGTGACG ATGCCGTTGA CACGGAGGGA GCAGCTGCTG AGACACATTA TACATATTTC 
CCCAGCACCG CAGTGGGAGA TGGGTCAGGG GGTACCACAT CTGGGAGTAC TACAGCTGTT 
GTTACCACCC AGGGCTCAGA GGCACTACTG GGGCAGGCAA CCCCGCCCAG CACAGGTCAA 
TTCTTTGTGA TGATGTCACC ACAAGAAGTA TTGCAGGGAG GGAGCCAGCG ATCGATTGCC 
CCCAGGACCC ACCCTTATTC CCCGAAGTCA GAGGCTCCCA GGACAACTCG AGATGAGAAA 
CGGAGGGCTC AACATAACGA AGTGGAGCGC CGCCGCCGGG ACAAGATCAA CAACTGGATT 
GTACAGCTGT CCAAAATCAT CCCAGACTGC TCTATGGAGA GCACCAAGTC TGGCCAGAGT 
AAAGGTGGAA TCCTGTCCAA AGCCTGTGAT TATATCCAGG AGCTGCGGCA GAGCAACCAC 
CGGCTGTCTG AAGAGCTGCA GGGGTTAGAT CAGTTGCAGC TGGACAACGA TGTGCTCCGG 
CAACAGGTGG AAGATCTCAA GAACAAGAAC CTGCTCCTGC GAGCTCAGCT TCGGCACCAC 
GGACTCGAGG TCGTCATCAA GAATGACAGC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usf1 ( XM_030253055.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108915.1 Mus musculus upstream transcription factor 1 (Usf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu69022
Clone ID Related Accession (Same CDS sequence)	XM_030253053.1 , XM_030253055.1 , NM_001305678.1
Accession Version	XM_030253055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	upstream stimulatory factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGGG TGATCCAGGT GTCAGAGGGG CAGCTGGATG GCCAGACAGA GGGCTCTGGC 
GCCATCAGTG GTTACCCTGC CACTCAGTCT ATGACCCAGG CAGTGATCCA GGGAGCTTTC 
ACCAGTGACG ATGCCGTTGA CACGGAGGGA GCAGCTGCTG AGACACATTA TACATATTTC 
CCCAGCACCG CAGTGGGAGA TGGGTCAGGG GGTACCACAT CTGGGAGTAC TACAGCTGTT 
GTTACCACCC AGGGCTCAGA GGCACTACTG GGGCAGGCAA CCCCGCCCAG CACAGGTCAA 
TTCTTTGTGA TGATGTCACC ACAAGAAGTA TTGCAGGGAG GGAGCCAGCG ATCGATTGCC 
CCCAGGACCC ACCCTTATTC CCCGAAGTCA GAGGCTCCCA GGACAACTCG AGATGAGAAA 
CGGAGGGCTC AACATAACGA AGTGGAGCGC CGCCGCCGGG ACAAGATCAA CAACTGGATT 
GTACAGCTGT CCAAAATCAT CCCAGACTGC TCTATGGAGA GCACCAAGTC TGGCCAGAGT 
AAAGGTGGAA TCCTGTCCAA AGCCTGTGAT TATATCCAGG AGCTGCGGCA GAGCAACCAC 
CGGCTGTCTG AAGAGCTGCA GGGGTTAGAT CAGTTGCAGC TGGACAACGA TGTGCTCCGG 
CAACAGGTGG AAGATCTCAA GAACAAGAAC CTGCTCCTGC GAGCTCAGCT TCGGCACCAC 
GGACTCGAGG TCGTCATCAA GAATGACAGC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108915.1
CDS	513..1268
Translation	MYRVIQVSEGQLDGQTEGSGAISGYPATQSMTQAVIQGAFTSDDAVDTEGAAAETHYTYFPSTAVGDGSGGTTSGSTTAVVTTQGSEALLGQATPPSTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

Target ORF information:

RefSeq Version	XM_030253055.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus upstream transcription factor 1 (Usf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253055.1

ORF Insert Sequence:

ATGTACAGGG TGATCCAGGT GTCAGAGGGG CAGCTGGATG GCCAGACAGA GGGCTCTGGC 
GCCATCAGTG GTTACCCTGC CACTCAGTCT ATGACCCAGG CAGTGATCCA GGGAGCTTTC 
ACCAGTGACG ATGCCGTTGA CACGGAGGGA GCAGCTGCTG AGACACATTA TACATATTTC 
CCCAGCACCG CAGTGGGAGA TGGGTCAGGG GGTACCACAT CTGGGAGTAC TACAGCTGTT 
GTTACCACCC AGGGCTCAGA GGCACTACTG GGGCAGGCAA CCCCGCCCAG CACAGGTCAA 
TTCTTTGTGA TGATGTCACC ACAAGAAGTA TTGCAGGGAG GGAGCCAGCG ATCGATTGCC 
CCCAGGACCC ACCCTTATTC CCCGAAGTCA GAGGCTCCCA GGACAACTCG AGATGAGAAA 
CGGAGGGCTC AACATAACGA AGTGGAGCGC CGCCGCCGGG ACAAGATCAA CAACTGGATT 
GTACAGCTGT CCAAAATCAT CCCAGACTGC TCTATGGAGA GCACCAAGTC TGGCCAGAGT 
AAAGGTGGAA TCCTGTCCAA AGCCTGTGAT TATATCCAGG AGCTGCGGCA GAGCAACCAC 
CGGCTGTCTG AAGAGCTGCA GGGGTTAGAT CAGTTGCAGC TGGACAACGA TGTGCTCCGG 
CAACAGGTGG AAGATCTCAA GAACAAGAAC CTGCTCCTGC GAGCTCAGCT TCGGCACCAC 
GGACTCGAGG TCGTCATCAA GAATGACAGC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overlapping roles and asymmetrical cross-regulation of the USF proteins in mice.
Proceedings of the National Academy of Sciences of the United States of America95(7)3758-63(1998 Mar)
Sirito M,Lin Q,Deng JM,Behringer RR,Sawadogo M

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Usf1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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