Uba1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Uba1
Entrez Gene ID	22201
Full Name	ubiquitin-like modifier activating enzyme 1
Synonyms	A1S9,Sbx,Ube-1,Ube1x
General protein information	Preferred Names ubiquitin-like modifier activating enzyme 1 Names ubiquitin-like modifier-activating enzyme 1 ubiquitin-activating enzyme E1, Chr X
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X A1.3\|X 16.15 cM
Summary	This gene encodes a member of the ubiquitin-activating E1 family. The encoded protein initiates the ubiquitin activation and transfer cascade, catalyzing the first step in ubiquitin conjugation to mark cellular proteins for proteasome degradation. Ubiquitin activating enzymes use ATP to form a thioester between a conserved catalytic cysteine of the enzyme and the C-terminal carboxylate of ubiquitin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276317.1	NP_001263246.1	ubiquitin-like modifier-activating enzyme 1 isoform 2
XM_030251302.1	XP_030107162.1	ubiquitin-like modifier-activating enzyme 1 isoform X1
NM_001276316.1	NP_001263245.1	ubiquitin-like modifier-activating enzyme 1 isoform 2
NM_009457.4	NP_033483.2	ubiquitin-like modifier-activating enzyme 1 isoform 1
NM_001136085.2	NP_001129557.1	ubiquitin-like modifier-activating enzyme 1 isoform 2

Pathways from BioSystems

Homologies

	UBA1	XP_001092372.2
Mus musculus (house mouse)	Uba1	NP_033483.2
Drosophila melanogaster (fruit fly)	Uba1	NP_477310.2
Saccharomyces cerevisiae (baker's yeast)	UBA1	NP_012712.1
Homo sapiens (human)	UBA1	NP_003325.2
Canis lupus familiaris (dog)	UBA1	XP_005641334.1
Bos taurus (cattle)	UBA1	NP_001095947.1
Danio rerio (zebrafish)	uba1	NP_998227.1
Caenorhabditis elegans (roundworm)	uba-1	NP_001033404.1
Oryza sativa (rice)	Os03g0294900	NP_001049821.1
Schizosaccharomyces pombe (fission yeast)	ptr3	XP_001713148.1
Magnaporthe oryzae (rice blast fungus)	MGG_01409	XP_003714354.1
Oryza sativa (rice)	Os07g0692900	NP_001060725.1
Rattus norvegicus (Norway rat)	Uba1	NP_001014102.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011872	XP_552371.3
Kluyveromyces lactis	KLLA0B14278g	XP_452166.1
Eremothecium gossypii	AGOS_AFR433C	NP_985980.1
Neurospora crassa	NCU04370	XP_956143.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread and dynamic translational control of red blood cell development.
Alvarez-Dominguez JR, Zhang X, Hu W
Blood129(5)619-629(2017 02)

Ubiquitin-activating enzyme activity contributes to differential accumulation of mutant huntingtin in brain and peripheral tissues.
Wade BE, Wang CE, Yan S, Bhat K, Huang B, Li S, Li XJ
The Journal of neuroscience : the official journal of the Society for Neuroscience34(25)8411-22(2014 Jun)

Ubiquitin-activating enzyme E1 inhibitor PYR41 attenuates angiotensin II-induced activation of dendritic cells via the I?Ba/NF-?B and MKP1/ERK/STAT1 pathways.
Chen C, Meng Y, Wang L, Wang HX, Tian C, Pang GD, Li HH, Du J
Immunology142(2)307-19(2014 Jun)

Dysregulation of ubiquitin homeostasis and ?-catenin signaling promote spinal muscular atrophy.
Wishart TM, Mutsaers CA, Riessland M, Reimer MM, Hunter G, Hannam ML, Eaton SL, Fuller HR, Roche SL, Somers E, Morse R, Young PJ, Lamont DJ, Hammerschmidt M, Joshi A, Hohenstein P, Morris GE, Parson SH, Skehel PA, Becker T, Robinson IM, Becker CG, Wirth B, Gillingwater TH
The Journal of clinical investigation124(4)1821-34(2014 Apr)

Epigenetic functions of smchd1 repress gene clusters on the inactive X chromosome and on autosomes.
Gendrel AV, Tang YA, Suzuki M, Godwin J, Nesterova TB, Greally JM, Heard E, Brockdorff N
Molecular and cellular biology33(16)3150-65(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Inhibition of ubiquitin-activating enzyme E1 (Ube1) increases the levels of high- molecular weight mouse Htt in relatively unaffected tissues, declining in the nuclear fraction with age.
Title: Ubiquitin-activating enzyme activity contributes to differential accumulation of mutant huntingtin in brain and peripheral tissues.

Dysregulation of UBA1 and subsequent ubiquitination pathways led to beta-catenin accumulation in a model of spinal muscular atrophy.
Title: Dysregulation of ubiquitin homeostasis and ?-catenin signaling promote spinal muscular atrophy.

These data suggest that Cys-278 can affect Ub charging through a change in the structural conformation of UBA1, not through direct interaction at the UBA1-Ub interface.
Title: The functional role of UBA1 cysteine-278 in ubiquitination.

E1 ligase of the proteasome is inhibitd by ziram and has a role in preventing dopaminergic cell damage
Title: Ziram causes dopaminergic cell damage by inhibiting E1 ligase of the proteasome.

biochemical and crystallographic characterization of the two half-domains and the crystal structure of the larger, second catalytic cysteine half-domain of mouse ubiquitin-activating enzyme
Title: Crystal structure of a fragment of mouse ubiquitin-activating enzyme.

The following Uba1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Uba1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15938	NM_001276317.1 Latest version!	Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu15938	NM_001276316.1 Latest version!	Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu15938	NM_001136085.2 Latest version!	Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu15938	XM_030251302.1 Latest version!	Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu04198	NM_009457.4 Latest version!	Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$622.30 $889.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Uba1 ( NM_001276317.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001263246.1 Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu15938
Clone ID Related Accession (Same CDS sequence)	NM_001276317.1 , XM_030251302.1 , NM_001276316.1 , NM_001136085.2
Accession Version	NM_001276317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	ubiquitin-like modifier-activating enzyme 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB858760.1 and AK143416.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 3. Variants 2, 3, and 4 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1660819.92771.1, SRR1660817.249766.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGCT CCCCTGCACA GTCTGCGCTG TCCGAAGTGT CCTCAGTGCC AACCAACGGA 
ATGGCGAAGA ACGGCAGTGA AGCAGACATA GACGAGAGCC TTTACTCCCG GCAGCTGTAC 
GTTTTGGGCC ATGAGGCAAT GAAAATGCTC CAGACATCCA GCGTCCTTGT CTCAGGCTTG 
CGGGGCTTGG GTGTAGAAAT TGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTCACC 
CTACATGACC AAGGAACTAC CCAGTGGGCT GATCTCTCCT CCCAGTTTTA CCTTCGGGAG 
GAGGACATTG GTAAAAATCG AGCGGAGGTA TCCCAGCCCC GACTTGCTGA ACTCAACAGC 
TATGTACCTG TCACTGCCTA CACTGGGCCT CTTGTCGAGG ACTTCCTTAG TAGCTTCCAG 
GTGGTGGTCC TCACCAACAG CCCCCTGGAA GCCCAGCTGC GAGTGGGGGA GTTCTGTCAT 
AGCCGTGGTA TCAAGCTAGT GGTGGCAGAT ACAAGAGGCC TGTTTGGGCA ACTTTTCTGT 
GATTTTGGAG AGGAAATGGT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG 
GTTTCAATGG TCACCAAGGA CAACCCCGGT GTGGTTACCT GCCTTGATGA GGCCCGACAT 
GGCTTTGAGA CTGGTGACTT CGTCTCATTC TCAGAAGTAC AGGGCATGAT CCAACTCAAT 
GGATGTCAGC CCATGGAGAT CAAAGTGCTG GGTCCTTATA CCTTTAGTAT CTGTGACACT 
TCCAACTTCT CTGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCGAAAAAG 
ATTAGTTTTA AATCCTTGCC AGCATCACTG GTAGAGCCTG ACTTTGTGAT GACTGACTTT 
GCCAAGTATT CTCGCCCTGC CCAACTGCAC ATTGGCTTCC AAGCTCTGCA CCAATTCTGT 
GCTCTGCACA ACCAACCACC TCGACCACGA AATGAGGAAG ATGCAACAGA GCTGGTGGGC 
CTGGCTCAGG CTGTAAACGC TCGGTCCCCA CCTTCAGTAA AACAGAACAG CTTGGATGAA 
GACCTTATTC GGAAGCTAGC TTATGTTGCT GCTGGGGACC TGGCACCCAT AAATGCTTTC 
ATTGGGGGCC TTGCTGCCCA GGAAGTCATG AAGGCCTGCT CTGGAAAGTT TATGCCCATC 
ATGCAGTGGT TGTACTTTGA TGCTCTTGAA TGTCTCCCAG AGGACAAAGA GGCTCTGACA 
GAGGAGAAGT GCCTCCCACG TCAGAACCGT TACGATGGGC AGGTAGCTGT ATTTGGGTCA 
GACTTTCAGG AGAAGCTGAG CAAGCAAAAG TACTTCCTGG TGGGTGCAGG GGCCATTGGC 
TGTGAATTGC TCAAGAACTT TGCCATGATT GGGCTGGGTT GTGGAGAGGG TGGAGAGGTC 
GTGGTCACAG ACATGGACAC CATTGAGAAA TCAAATCTGA ACCGACAGTT TCTCTTCCGG 
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCCGCTG CAGCTGTGCG CCAGATGAAT 
CCTTACATCC AGGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA TTTGGATGGT GTGGCCAATG CTCTGGACAA CATAGATGCC 
CGCATGTACA TGGATCGCCG ATGTGTGTAC TATCGGAAGC CACTGCTAGA GTCTGGCACA 
CTGGGCACAA AGGGCAACGT GCAGGTGGTA ATCCCCTTCC TGACAGAATC CTACAGCTCT 
AGCCAGGATC CACCAGAGAA ATCCATCCCC ATTTGTACCC TGAAAAACTT TCCCAATGCC 
ATCGAACACA CTCTTCAGTG GGCCCGGGAT GAATTTGAAG GCCTTTTCAA GCAGCCAGCA 
GAAAATGTTA ATCAGTACCT CACAGACTCC AAATTTGTGG AGCGGACATT GCGGCTGGCT 
GGTACCCAGC CATTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGTT GCAGCGACCA 
CAGACTTGGG GAGACTGTGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTGTAAC 
AACATCCGGC AACTGCTGCA CAACTTTCCT CCTGACCAGC TCACCAGCTC AGGGGCCCCT 
TTCTGGTCTG GACCCAAACG CTGTCCACAC CCACTTACTT TTGATGTTAA CAATACATTG 
CATCTGGATT ATGTGATGGC TGCTGCCAAC CTTTTTGCCC AGACCTATGG GTTGACTGGA 
TCCCAAGACC GAGCTGCTGT GGCCTCACTC CTGCAGTCAG TACAAGTCCC AGAGTTCACC 
CCCAAGTCTG GTGTCAAGAT TCATGTTTCT GACCAGGAGT TGCAGAGTGC CAATGCCTCT 
GTTGATGACA GCCGTCTTGA GGAGCTCAAA GCCACATTGC CCAGCCCAGA CAAGTTACCC 
GGATTTAAGA TGTACCCCAT TGATTTTGAG AAGGATGATG ACAGCAATTT CCACATGGAT 
TTCATTGTGG CTGCATCCAA TCTTCGGGCC GAAAACTATG ATATTTCCCC TGCAGACCGA 
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACAAC CACAGCTGCT 
GTGGTTGGCC TTGTGTGTCT GGAGCTCTAC AAGGTAGTTC AAGGGCACCA ACAGCTCGAT 
TCCTACAAAA ATGGTTTCCT GAACTTGGCC CTGCCCTTCT TTGGGTTTTC TGAACCTCTT 
GCTGCACCTC GTCACCAGTA CTATAATCAA GAGTGGACAT TGTGGGATCG CTTTGAAGTA 
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAGC AGTTCCTTGA TTACTTTAAG 
ACAGAGCACA AATTGGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCTTTC 
TTCATGCCAG CTGCTAAGCT CAAGGAACGA TTGGATCAGC CGATGACAGA GATTGTGAGC 
CGAGTGTCAA AGAGAAAGCT GGGCCGCCAT GTGCGGGCAC TGGTGCTTGA GCTGTGCTGC 
AACGATGAAA GCGGCGAGGA CGTCGAGGTC CCTTATGTCC GATATACCAT TCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263246.1
CDS	226..3402
Translation	MSSSPLSKKRRVSGPDPKPGSNCSPAQSALSEVSSVPTNGMAKNGSEADIDESLYSRQLYVLGHEAMKMLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTTQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSSFQVVVLTNSPLEAQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMVLTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFETGDFVSFSEVQGMIQLNGCQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLVEPDFVMTDFAKYSRPAQLHIGFQALHQFCALHNQPPRPRNEEDATELVGLAQAVNARSPPSVKQNSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEEKCLPRQNRYDGQVAVFGSDFQEKLSKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEVVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPYIQVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNIDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDSKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWGDCVTWACHHWHTQYCNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNTLHLDYVMAAANLFAQTYGLTGSQDRAAVASLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	NM_001276317.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276317.1

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGCT CCCCTGCACA GTCTGCGCTG TCCGAAGTGT CCTCAGTGCC AACCAACGGA 
ATGGCGAAGA ACGGCAGTGA AGCAGACATA GACGAGAGCC TTTACTCCCG GCAGCTGTAC 
GTTTTGGGCC ATGAGGCAAT GAAAATGCTC CAGACATCCA GCGTCCTTGT CTCAGGCTTG 
CGGGGCTTGG GTGTAGAAAT TGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTCACC 
CTACATGACC AAGGAACTAC CCAGTGGGCT GATCTCTCCT CCCAGTTTTA CCTTCGGGAG 
GAGGACATTG GTAAAAATCG AGCGGAGGTA TCCCAGCCCC GACTTGCTGA ACTCAACAGC 
TATGTACCTG TCACTGCCTA CACTGGGCCT CTTGTCGAGG ACTTCCTTAG TAGCTTCCAG 
GTGGTGGTCC TCACCAACAG CCCCCTGGAA GCCCAGCTGC GAGTGGGGGA GTTCTGTCAT 
AGCCGTGGTA TCAAGCTAGT GGTGGCAGAT ACAAGAGGCC TGTTTGGGCA ACTTTTCTGT 
GATTTTGGAG AGGAAATGGT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG 
GTTTCAATGG TCACCAAGGA CAACCCCGGT GTGGTTACCT GCCTTGATGA GGCCCGACAT 
GGCTTTGAGA CTGGTGACTT CGTCTCATTC TCAGAAGTAC AGGGCATGAT CCAACTCAAT 
GGATGTCAGC CCATGGAGAT CAAAGTGCTG GGTCCTTATA CCTTTAGTAT CTGTGACACT 
TCCAACTTCT CTGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCGAAAAAG 
ATTAGTTTTA AATCCTTGCC AGCATCACTG GTAGAGCCTG ACTTTGTGAT GACTGACTTT 
GCCAAGTATT CTCGCCCTGC CCAACTGCAC ATTGGCTTCC AAGCTCTGCA CCAATTCTGT 
GCTCTGCACA ACCAACCACC TCGACCACGA AATGAGGAAG ATGCAACAGA GCTGGTGGGC 
CTGGCTCAGG CTGTAAACGC TCGGTCCCCA CCTTCAGTAA AACAGAACAG CTTGGATGAA 
GACCTTATTC GGAAGCTAGC TTATGTTGCT GCTGGGGACC TGGCACCCAT AAATGCTTTC 
ATTGGGGGCC TTGCTGCCCA GGAAGTCATG AAGGCCTGCT CTGGAAAGTT TATGCCCATC 
ATGCAGTGGT TGTACTTTGA TGCTCTTGAA TGTCTCCCAG AGGACAAAGA GGCTCTGACA 
GAGGAGAAGT GCCTCCCACG TCAGAACCGT TACGATGGGC AGGTAGCTGT ATTTGGGTCA 
GACTTTCAGG AGAAGCTGAG CAAGCAAAAG TACTTCCTGG TGGGTGCAGG GGCCATTGGC 
TGTGAATTGC TCAAGAACTT TGCCATGATT GGGCTGGGTT GTGGAGAGGG TGGAGAGGTC 
GTGGTCACAG ACATGGACAC CATTGAGAAA TCAAATCTGA ACCGACAGTT TCTCTTCCGG 
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCCGCTG CAGCTGTGCG CCAGATGAAT 
CCTTACATCC AGGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA TTTGGATGGT GTGGCCAATG CTCTGGACAA CATAGATGCC 
CGCATGTACA TGGATCGCCG ATGTGTGTAC TATCGGAAGC CACTGCTAGA GTCTGGCACA 
CTGGGCACAA AGGGCAACGT GCAGGTGGTA ATCCCCTTCC TGACAGAATC CTACAGCTCT 
AGCCAGGATC CACCAGAGAA ATCCATCCCC ATTTGTACCC TGAAAAACTT TCCCAATGCC 
ATCGAACACA CTCTTCAGTG GGCCCGGGAT GAATTTGAAG GCCTTTTCAA GCAGCCAGCA 
GAAAATGTTA ATCAGTACCT CACAGACTCC AAATTTGTGG AGCGGACATT GCGGCTGGCT 
GGTACCCAGC CATTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGTT GCAGCGACCA 
CAGACTTGGG GAGACTGTGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTGTAAC 
AACATCCGGC AACTGCTGCA CAACTTTCCT CCTGACCAGC TCACCAGCTC AGGGGCCCCT 
TTCTGGTCTG GACCCAAACG CTGTCCACAC CCACTTACTT TTGATGTTAA CAATACATTG 
CATCTGGATT ATGTGATGGC TGCTGCCAAC CTTTTTGCCC AGACCTATGG GTTGACTGGA 
TCCCAAGACC GAGCTGCTGT GGCCTCACTC CTGCAGTCAG TACAAGTCCC AGAGTTCACC 
CCCAAGTCTG GTGTCAAGAT TCATGTTTCT GACCAGGAGT TGCAGAGTGC CAATGCCTCT 
GTTGATGACA GCCGTCTTGA GGAGCTCAAA GCCACATTGC CCAGCCCAGA CAAGTTACCC 
GGATTTAAGA TGTACCCCAT TGATTTTGAG AAGGATGATG ACAGCAATTT CCACATGGAT 
TTCATTGTGG CTGCATCCAA TCTTCGGGCC GAAAACTATG ATATTTCCCC TGCAGACCGA 
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACAAC CACAGCTGCT 
GTGGTTGGCC TTGTGTGTCT GGAGCTCTAC AAGGTAGTTC AAGGGCACCA ACAGCTCGAT 
TCCTACAAAA ATGGTTTCCT GAACTTGGCC CTGCCCTTCT TTGGGTTTTC TGAACCTCTT 
GCTGCACCTC GTCACCAGTA CTATAATCAA GAGTGGACAT TGTGGGATCG CTTTGAAGTA 
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAGC AGTTCCTTGA TTACTTTAAG 
ACAGAGCACA AATTGGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCTTTC 
TTCATGCCAG CTGCTAAGCT CAAGGAACGA TTGGATCAGC CGATGACAGA GATTGTGAGC 
CGAGTGTCAA AGAGAAAGCT GGGCCGCCAT GTGCGGGCAC TGGTGCTTGA GCTGTGCTGC 
AACGATGAAA GCGGCGAGGA CGTCGAGGTC CCTTATGTCC GATATACCAT TCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Uba1 ( NM_001276316.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001263245.1 Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu15938
Clone ID Related Accession (Same CDS sequence)	NM_001276317.1 , XM_030251302.1 , NM_001276316.1 , NM_001136085.2
Accession Version	NM_001276316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	ubiquitin-like modifier-activating enzyme 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL807240.12, BC145984.1 and AK143416.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the shorter isoform (2). Variants 2, 3, and 4 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1660819.92771.1, SRR1660817.249766.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGCT CCCCTGCACA GTCTGCGCTG TCCGAAGTGT CCTCAGTGCC AACCAACGGA 
ATGGCGAAGA ACGGCAGTGA AGCAGACATA GACGAGAGCC TTTACTCCCG GCAGCTGTAC 
GTTTTGGGCC ATGAGGCAAT GAAAATGCTC CAGACATCCA GCGTCCTTGT CTCAGGCTTG 
CGGGGCTTGG GTGTAGAAAT TGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTCACC 
CTACATGACC AAGGAACTAC CCAGTGGGCT GATCTCTCCT CCCAGTTTTA CCTTCGGGAG 
GAGGACATTG GTAAAAATCG AGCGGAGGTA TCCCAGCCCC GACTTGCTGA ACTCAACAGC 
TATGTACCTG TCACTGCCTA CACTGGGCCT CTTGTCGAGG ACTTCCTTAG TAGCTTCCAG 
GTGGTGGTCC TCACCAACAG CCCCCTGGAA GCCCAGCTGC GAGTGGGGGA GTTCTGTCAT 
AGCCGTGGTA TCAAGCTAGT GGTGGCAGAT ACAAGAGGCC TGTTTGGGCA ACTTTTCTGT 
GATTTTGGAG AGGAAATGGT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG 
GTTTCAATGG TCACCAAGGA CAACCCCGGT GTGGTTACCT GCCTTGATGA GGCCCGACAT 
GGCTTTGAGA CTGGTGACTT CGTCTCATTC TCAGAAGTAC AGGGCATGAT CCAACTCAAT 
GGATGTCAGC CCATGGAGAT CAAAGTGCTG GGTCCTTATA CCTTTAGTAT CTGTGACACT 
TCCAACTTCT CTGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCGAAAAAG 
ATTAGTTTTA AATCCTTGCC AGCATCACTG GTAGAGCCTG ACTTTGTGAT GACTGACTTT 
GCCAAGTATT CTCGCCCTGC CCAACTGCAC ATTGGCTTCC AAGCTCTGCA CCAATTCTGT 
GCTCTGCACA ACCAACCACC TCGACCACGA AATGAGGAAG ATGCAACAGA GCTGGTGGGC 
CTGGCTCAGG CTGTAAACGC TCGGTCCCCA CCTTCAGTAA AACAGAACAG CTTGGATGAA 
GACCTTATTC GGAAGCTAGC TTATGTTGCT GCTGGGGACC TGGCACCCAT AAATGCTTTC 
ATTGGGGGCC TTGCTGCCCA GGAAGTCATG AAGGCCTGCT CTGGAAAGTT TATGCCCATC 
ATGCAGTGGT TGTACTTTGA TGCTCTTGAA TGTCTCCCAG AGGACAAAGA GGCTCTGACA 
GAGGAGAAGT GCCTCCCACG TCAGAACCGT TACGATGGGC AGGTAGCTGT ATTTGGGTCA 
GACTTTCAGG AGAAGCTGAG CAAGCAAAAG TACTTCCTGG TGGGTGCAGG GGCCATTGGC 
TGTGAATTGC TCAAGAACTT TGCCATGATT GGGCTGGGTT GTGGAGAGGG TGGAGAGGTC 
GTGGTCACAG ACATGGACAC CATTGAGAAA TCAAATCTGA ACCGACAGTT TCTCTTCCGG 
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCCGCTG CAGCTGTGCG CCAGATGAAT 
CCTTACATCC AGGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA TTTGGATGGT GTGGCCAATG CTCTGGACAA CATAGATGCC 
CGCATGTACA TGGATCGCCG ATGTGTGTAC TATCGGAAGC CACTGCTAGA GTCTGGCACA 
CTGGGCACAA AGGGCAACGT GCAGGTGGTA ATCCCCTTCC TGACAGAATC CTACAGCTCT 
AGCCAGGATC CACCAGAGAA ATCCATCCCC ATTTGTACCC TGAAAAACTT TCCCAATGCC 
ATCGAACACA CTCTTCAGTG GGCCCGGGAT GAATTTGAAG GCCTTTTCAA GCAGCCAGCA 
GAAAATGTTA ATCAGTACCT CACAGACTCC AAATTTGTGG AGCGGACATT GCGGCTGGCT 
GGTACCCAGC CATTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGTT GCAGCGACCA 
CAGACTTGGG GAGACTGTGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTGTAAC 
AACATCCGGC AACTGCTGCA CAACTTTCCT CCTGACCAGC TCACCAGCTC AGGGGCCCCT 
TTCTGGTCTG GACCCAAACG CTGTCCACAC CCACTTACTT TTGATGTTAA CAATACATTG 
CATCTGGATT ATGTGATGGC TGCTGCCAAC CTTTTTGCCC AGACCTATGG GTTGACTGGA 
TCCCAAGACC GAGCTGCTGT GGCCTCACTC CTGCAGTCAG TACAAGTCCC AGAGTTCACC 
CCCAAGTCTG GTGTCAAGAT TCATGTTTCT GACCAGGAGT TGCAGAGTGC CAATGCCTCT 
GTTGATGACA GCCGTCTTGA GGAGCTCAAA GCCACATTGC CCAGCCCAGA CAAGTTACCC 
GGATTTAAGA TGTACCCCAT TGATTTTGAG AAGGATGATG ACAGCAATTT CCACATGGAT 
TTCATTGTGG CTGCATCCAA TCTTCGGGCC GAAAACTATG ATATTTCCCC TGCAGACCGA 
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACAAC CACAGCTGCT 
GTGGTTGGCC TTGTGTGTCT GGAGCTCTAC AAGGTAGTTC AAGGGCACCA ACAGCTCGAT 
TCCTACAAAA ATGGTTTCCT GAACTTGGCC CTGCCCTTCT TTGGGTTTTC TGAACCTCTT 
GCTGCACCTC GTCACCAGTA CTATAATCAA GAGTGGACAT TGTGGGATCG CTTTGAAGTA 
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAGC AGTTCCTTGA TTACTTTAAG 
ACAGAGCACA AATTGGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCTTTC 
TTCATGCCAG CTGCTAAGCT CAAGGAACGA TTGGATCAGC CGATGACAGA GATTGTGAGC 
CGAGTGTCAA AGAGAAAGCT GGGCCGCCAT GTGCGGGCAC TGGTGCTTGA GCTGTGCTGC 
AACGATGAAA GCGGCGAGGA CGTCGAGGTC CCTTATGTCC GATATACCAT TCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263245.1
CDS	323..3499
Translation	MSSSPLSKKRRVSGPDPKPGSNCSPAQSALSEVSSVPTNGMAKNGSEADIDESLYSRQLYVLGHEAMKMLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTTQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSSFQVVVLTNSPLEAQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMVLTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFETGDFVSFSEVQGMIQLNGCQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLVEPDFVMTDFAKYSRPAQLHIGFQALHQFCALHNQPPRPRNEEDATELVGLAQAVNARSPPSVKQNSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEEKCLPRQNRYDGQVAVFGSDFQEKLSKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEVVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPYIQVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNIDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDSKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWGDCVTWACHHWHTQYCNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNTLHLDYVMAAANLFAQTYGLTGSQDRAAVASLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	NM_001276316.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276316.1

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGCT CCCCTGCACA GTCTGCGCTG TCCGAAGTGT CCTCAGTGCC AACCAACGGA 
ATGGCGAAGA ACGGCAGTGA AGCAGACATA GACGAGAGCC TTTACTCCCG GCAGCTGTAC 
GTTTTGGGCC ATGAGGCAAT GAAAATGCTC CAGACATCCA GCGTCCTTGT CTCAGGCTTG 
CGGGGCTTGG GTGTAGAAAT TGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTCACC 
CTACATGACC AAGGAACTAC CCAGTGGGCT GATCTCTCCT CCCAGTTTTA CCTTCGGGAG 
GAGGACATTG GTAAAAATCG AGCGGAGGTA TCCCAGCCCC GACTTGCTGA ACTCAACAGC 
TATGTACCTG TCACTGCCTA CACTGGGCCT CTTGTCGAGG ACTTCCTTAG TAGCTTCCAG 
GTGGTGGTCC TCACCAACAG CCCCCTGGAA GCCCAGCTGC GAGTGGGGGA GTTCTGTCAT 
AGCCGTGGTA TCAAGCTAGT GGTGGCAGAT ACAAGAGGCC TGTTTGGGCA ACTTTTCTGT 
GATTTTGGAG AGGAAATGGT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG 
GTTTCAATGG TCACCAAGGA CAACCCCGGT GTGGTTACCT GCCTTGATGA GGCCCGACAT 
GGCTTTGAGA CTGGTGACTT CGTCTCATTC TCAGAAGTAC AGGGCATGAT CCAACTCAAT 
GGATGTCAGC CCATGGAGAT CAAAGTGCTG GGTCCTTATA CCTTTAGTAT CTGTGACACT 
TCCAACTTCT CTGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCGAAAAAG 
ATTAGTTTTA AATCCTTGCC AGCATCACTG GTAGAGCCTG ACTTTGTGAT GACTGACTTT 
GCCAAGTATT CTCGCCCTGC CCAACTGCAC ATTGGCTTCC AAGCTCTGCA CCAATTCTGT 
GCTCTGCACA ACCAACCACC TCGACCACGA AATGAGGAAG ATGCAACAGA GCTGGTGGGC 
CTGGCTCAGG CTGTAAACGC TCGGTCCCCA CCTTCAGTAA AACAGAACAG CTTGGATGAA 
GACCTTATTC GGAAGCTAGC TTATGTTGCT GCTGGGGACC TGGCACCCAT AAATGCTTTC 
ATTGGGGGCC TTGCTGCCCA GGAAGTCATG AAGGCCTGCT CTGGAAAGTT TATGCCCATC 
ATGCAGTGGT TGTACTTTGA TGCTCTTGAA TGTCTCCCAG AGGACAAAGA GGCTCTGACA 
GAGGAGAAGT GCCTCCCACG TCAGAACCGT TACGATGGGC AGGTAGCTGT ATTTGGGTCA 
GACTTTCAGG AGAAGCTGAG CAAGCAAAAG TACTTCCTGG TGGGTGCAGG GGCCATTGGC 
TGTGAATTGC TCAAGAACTT TGCCATGATT GGGCTGGGTT GTGGAGAGGG TGGAGAGGTC 
GTGGTCACAG ACATGGACAC CATTGAGAAA TCAAATCTGA ACCGACAGTT TCTCTTCCGG 
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCCGCTG CAGCTGTGCG CCAGATGAAT 
CCTTACATCC AGGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA TTTGGATGGT GTGGCCAATG CTCTGGACAA CATAGATGCC 
CGCATGTACA TGGATCGCCG ATGTGTGTAC TATCGGAAGC CACTGCTAGA GTCTGGCACA 
CTGGGCACAA AGGGCAACGT GCAGGTGGTA ATCCCCTTCC TGACAGAATC CTACAGCTCT 
AGCCAGGATC CACCAGAGAA ATCCATCCCC ATTTGTACCC TGAAAAACTT TCCCAATGCC 
ATCGAACACA CTCTTCAGTG GGCCCGGGAT GAATTTGAAG GCCTTTTCAA GCAGCCAGCA 
GAAAATGTTA ATCAGTACCT CACAGACTCC AAATTTGTGG AGCGGACATT GCGGCTGGCT 
GGTACCCAGC CATTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGTT GCAGCGACCA 
CAGACTTGGG GAGACTGTGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTGTAAC 
AACATCCGGC AACTGCTGCA CAACTTTCCT CCTGACCAGC TCACCAGCTC AGGGGCCCCT 
TTCTGGTCTG GACCCAAACG CTGTCCACAC CCACTTACTT TTGATGTTAA CAATACATTG 
CATCTGGATT ATGTGATGGC TGCTGCCAAC CTTTTTGCCC AGACCTATGG GTTGACTGGA 
TCCCAAGACC GAGCTGCTGT GGCCTCACTC CTGCAGTCAG TACAAGTCCC AGAGTTCACC 
CCCAAGTCTG GTGTCAAGAT TCATGTTTCT GACCAGGAGT TGCAGAGTGC CAATGCCTCT 
GTTGATGACA GCCGTCTTGA GGAGCTCAAA GCCACATTGC CCAGCCCAGA CAAGTTACCC 
GGATTTAAGA TGTACCCCAT TGATTTTGAG AAGGATGATG ACAGCAATTT CCACATGGAT 
TTCATTGTGG CTGCATCCAA TCTTCGGGCC GAAAACTATG ATATTTCCCC TGCAGACCGA 
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACAAC CACAGCTGCT 
GTGGTTGGCC TTGTGTGTCT GGAGCTCTAC AAGGTAGTTC AAGGGCACCA ACAGCTCGAT 
TCCTACAAAA ATGGTTTCCT GAACTTGGCC CTGCCCTTCT TTGGGTTTTC TGAACCTCTT 
GCTGCACCTC GTCACCAGTA CTATAATCAA GAGTGGACAT TGTGGGATCG CTTTGAAGTA 
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAGC AGTTCCTTGA TTACTTTAAG 
ACAGAGCACA AATTGGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCTTTC 
TTCATGCCAG CTGCTAAGCT CAAGGAACGA TTGGATCAGC CGATGACAGA GATTGTGAGC 
CGAGTGTCAA AGAGAAAGCT GGGCCGCCAT GTGCGGGCAC TGGTGCTTGA GCTGTGCTGC 
AACGATGAAA GCGGCGAGGA CGTCGAGGTC CCTTATGTCC GATATACCAT TCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Uba1 ( NM_001136085.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001129557.1 Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 2, mRNA.

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CloneID	OMu15938
Clone ID Related Accession (Same CDS sequence)	NM_001276317.1 , XM_030251302.1 , NM_001276316.1 , NM_001136085.2
Accession Version	NM_001136085.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	ubiquitin-like modifier-activating enzyme 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY316912.1 and AK143416.1. On Jan 30, 2013 this sequence version replaced NM_001136085.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 3. Variants 2, 3, and 4 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK143416.1, SRR1660821.141401.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGCT CCCCTGCACA GTCTGCGCTG TCCGAAGTGT CCTCAGTGCC AACCAACGGA 
ATGGCGAAGA ACGGCAGTGA AGCAGACATA GACGAGAGCC TTTACTCCCG GCAGCTGTAC 
GTTTTGGGCC ATGAGGCAAT GAAAATGCTC CAGACATCCA GCGTCCTTGT CTCAGGCTTG 
CGGGGCTTGG GTGTAGAAAT TGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTCACC 
CTACATGACC AAGGAACTAC CCAGTGGGCT GATCTCTCCT CCCAGTTTTA CCTTCGGGAG 
GAGGACATTG GTAAAAATCG AGCGGAGGTA TCCCAGCCCC GACTTGCTGA ACTCAACAGC 
TATGTACCTG TCACTGCCTA CACTGGGCCT CTTGTCGAGG ACTTCCTTAG TAGCTTCCAG 
GTGGTGGTCC TCACCAACAG CCCCCTGGAA GCCCAGCTGC GAGTGGGGGA GTTCTGTCAT 
AGCCGTGGTA TCAAGCTAGT GGTGGCAGAT ACAAGAGGCC TGTTTGGGCA ACTTTTCTGT 
GATTTTGGAG AGGAAATGGT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG 
GTTTCAATGG TCACCAAGGA CAACCCCGGT GTGGTTACCT GCCTTGATGA GGCCCGACAT 
GGCTTTGAGA CTGGTGACTT CGTCTCATTC TCAGAAGTAC AGGGCATGAT CCAACTCAAT 
GGATGTCAGC CCATGGAGAT CAAAGTGCTG GGTCCTTATA CCTTTAGTAT CTGTGACACT 
TCCAACTTCT CTGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCGAAAAAG 
ATTAGTTTTA AATCCTTGCC AGCATCACTG GTAGAGCCTG ACTTTGTGAT GACTGACTTT 
GCCAAGTATT CTCGCCCTGC CCAACTGCAC ATTGGCTTCC AAGCTCTGCA CCAATTCTGT 
GCTCTGCACA ACCAACCACC TCGACCACGA AATGAGGAAG ATGCAACAGA GCTGGTGGGC 
CTGGCTCAGG CTGTAAACGC TCGGTCCCCA CCTTCAGTAA AACAGAACAG CTTGGATGAA 
GACCTTATTC GGAAGCTAGC TTATGTTGCT GCTGGGGACC TGGCACCCAT AAATGCTTTC 
ATTGGGGGCC TTGCTGCCCA GGAAGTCATG AAGGCCTGCT CTGGAAAGTT TATGCCCATC 
ATGCAGTGGT TGTACTTTGA TGCTCTTGAA TGTCTCCCAG AGGACAAAGA GGCTCTGACA 
GAGGAGAAGT GCCTCCCACG TCAGAACCGT TACGATGGGC AGGTAGCTGT ATTTGGGTCA 
GACTTTCAGG AGAAGCTGAG CAAGCAAAAG TACTTCCTGG TGGGTGCAGG GGCCATTGGC 
TGTGAATTGC TCAAGAACTT TGCCATGATT GGGCTGGGTT GTGGAGAGGG TGGAGAGGTC 
GTGGTCACAG ACATGGACAC CATTGAGAAA TCAAATCTGA ACCGACAGTT TCTCTTCCGG 
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCCGCTG CAGCTGTGCG CCAGATGAAT 
CCTTACATCC AGGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA TTTGGATGGT GTGGCCAATG CTCTGGACAA CATAGATGCC 
CGCATGTACA TGGATCGCCG ATGTGTGTAC TATCGGAAGC CACTGCTAGA GTCTGGCACA 
CTGGGCACAA AGGGCAACGT GCAGGTGGTA ATCCCCTTCC TGACAGAATC CTACAGCTCT 
AGCCAGGATC CACCAGAGAA ATCCATCCCC ATTTGTACCC TGAAAAACTT TCCCAATGCC 
ATCGAACACA CTCTTCAGTG GGCCCGGGAT GAATTTGAAG GCCTTTTCAA GCAGCCAGCA 
GAAAATGTTA ATCAGTACCT CACAGACTCC AAATTTGTGG AGCGGACATT GCGGCTGGCT 
GGTACCCAGC CATTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGTT GCAGCGACCA 
CAGACTTGGG GAGACTGTGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTGTAAC 
AACATCCGGC AACTGCTGCA CAACTTTCCT CCTGACCAGC TCACCAGCTC AGGGGCCCCT 
TTCTGGTCTG GACCCAAACG CTGTCCACAC CCACTTACTT TTGATGTTAA CAATACATTG 
CATCTGGATT ATGTGATGGC TGCTGCCAAC CTTTTTGCCC AGACCTATGG GTTGACTGGA 
TCCCAAGACC GAGCTGCTGT GGCCTCACTC CTGCAGTCAG TACAAGTCCC AGAGTTCACC 
CCCAAGTCTG GTGTCAAGAT TCATGTTTCT GACCAGGAGT TGCAGAGTGC CAATGCCTCT 
GTTGATGACA GCCGTCTTGA GGAGCTCAAA GCCACATTGC CCAGCCCAGA CAAGTTACCC 
GGATTTAAGA TGTACCCCAT TGATTTTGAG AAGGATGATG ACAGCAATTT CCACATGGAT 
TTCATTGTGG CTGCATCCAA TCTTCGGGCC GAAAACTATG ATATTTCCCC TGCAGACCGA 
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACAAC CACAGCTGCT 
GTGGTTGGCC TTGTGTGTCT GGAGCTCTAC AAGGTAGTTC AAGGGCACCA ACAGCTCGAT 
TCCTACAAAA ATGGTTTCCT GAACTTGGCC CTGCCCTTCT TTGGGTTTTC TGAACCTCTT 
GCTGCACCTC GTCACCAGTA CTATAATCAA GAGTGGACAT TGTGGGATCG CTTTGAAGTA 
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAGC AGTTCCTTGA TTACTTTAAG 
ACAGAGCACA AATTGGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCTTTC 
TTCATGCCAG CTGCTAAGCT CAAGGAACGA TTGGATCAGC CGATGACAGA GATTGTGAGC 
CGAGTGTCAA AGAGAAAGCT GGGCCGCCAT GTGCGGGCAC TGGTGCTTGA GCTGTGCTGC 
AACGATGAAA GCGGCGAGGA CGTCGAGGTC CCTTATGTCC GATATACCAT TCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129557.1
CDS	231..3407
Translation	MSSSPLSKKRRVSGPDPKPGSNCSPAQSALSEVSSVPTNGMAKNGSEADIDESLYSRQLYVLGHEAMKMLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTTQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSSFQVVVLTNSPLEAQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMVLTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFETGDFVSFSEVQGMIQLNGCQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLVEPDFVMTDFAKYSRPAQLHIGFQALHQFCALHNQPPRPRNEEDATELVGLAQAVNARSPPSVKQNSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEEKCLPRQNRYDGQVAVFGSDFQEKLSKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEVVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPYIQVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNIDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDSKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWGDCVTWACHHWHTQYCNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNTLHLDYVMAAANLFAQTYGLTGSQDRAAVASLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	NM_001136085.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136085.2

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGCT CCCCTGCACA GTCTGCGCTG TCCGAAGTGT CCTCAGTGCC AACCAACGGA 
ATGGCGAAGA ACGGCAGTGA AGCAGACATA GACGAGAGCC TTTACTCCCG GCAGCTGTAC 
GTTTTGGGCC ATGAGGCAAT GAAAATGCTC CAGACATCCA GCGTCCTTGT CTCAGGCTTG 
CGGGGCTTGG GTGTAGAAAT TGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTCACC 
CTACATGACC AAGGAACTAC CCAGTGGGCT GATCTCTCCT CCCAGTTTTA CCTTCGGGAG 
GAGGACATTG GTAAAAATCG AGCGGAGGTA TCCCAGCCCC GACTTGCTGA ACTCAACAGC 
TATGTACCTG TCACTGCCTA CACTGGGCCT CTTGTCGAGG ACTTCCTTAG TAGCTTCCAG 
GTGGTGGTCC TCACCAACAG CCCCCTGGAA GCCCAGCTGC GAGTGGGGGA GTTCTGTCAT 
AGCCGTGGTA TCAAGCTAGT GGTGGCAGAT ACAAGAGGCC TGTTTGGGCA ACTTTTCTGT 
GATTTTGGAG AGGAAATGGT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG 
GTTTCAATGG TCACCAAGGA CAACCCCGGT GTGGTTACCT GCCTTGATGA GGCCCGACAT 
GGCTTTGAGA CTGGTGACTT CGTCTCATTC TCAGAAGTAC AGGGCATGAT CCAACTCAAT 
GGATGTCAGC CCATGGAGAT CAAAGTGCTG GGTCCTTATA CCTTTAGTAT CTGTGACACT 
TCCAACTTCT CTGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCGAAAAAG 
ATTAGTTTTA AATCCTTGCC AGCATCACTG GTAGAGCCTG ACTTTGTGAT GACTGACTTT 
GCCAAGTATT CTCGCCCTGC CCAACTGCAC ATTGGCTTCC AAGCTCTGCA CCAATTCTGT 
GCTCTGCACA ACCAACCACC TCGACCACGA AATGAGGAAG ATGCAACAGA GCTGGTGGGC 
CTGGCTCAGG CTGTAAACGC TCGGTCCCCA CCTTCAGTAA AACAGAACAG CTTGGATGAA 
GACCTTATTC GGAAGCTAGC TTATGTTGCT GCTGGGGACC TGGCACCCAT AAATGCTTTC 
ATTGGGGGCC TTGCTGCCCA GGAAGTCATG AAGGCCTGCT CTGGAAAGTT TATGCCCATC 
ATGCAGTGGT TGTACTTTGA TGCTCTTGAA TGTCTCCCAG AGGACAAAGA GGCTCTGACA 
GAGGAGAAGT GCCTCCCACG TCAGAACCGT TACGATGGGC AGGTAGCTGT ATTTGGGTCA 
GACTTTCAGG AGAAGCTGAG CAAGCAAAAG TACTTCCTGG TGGGTGCAGG GGCCATTGGC 
TGTGAATTGC TCAAGAACTT TGCCATGATT GGGCTGGGTT GTGGAGAGGG TGGAGAGGTC 
GTGGTCACAG ACATGGACAC CATTGAGAAA TCAAATCTGA ACCGACAGTT TCTCTTCCGG 
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCCGCTG CAGCTGTGCG CCAGATGAAT 
CCTTACATCC AGGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA TTTGGATGGT GTGGCCAATG CTCTGGACAA CATAGATGCC 
CGCATGTACA TGGATCGCCG ATGTGTGTAC TATCGGAAGC CACTGCTAGA GTCTGGCACA 
CTGGGCACAA AGGGCAACGT GCAGGTGGTA ATCCCCTTCC TGACAGAATC CTACAGCTCT 
AGCCAGGATC CACCAGAGAA ATCCATCCCC ATTTGTACCC TGAAAAACTT TCCCAATGCC 
ATCGAACACA CTCTTCAGTG GGCCCGGGAT GAATTTGAAG GCCTTTTCAA GCAGCCAGCA 
GAAAATGTTA ATCAGTACCT CACAGACTCC AAATTTGTGG AGCGGACATT GCGGCTGGCT 
GGTACCCAGC CATTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGTT GCAGCGACCA 
CAGACTTGGG GAGACTGTGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTGTAAC 
AACATCCGGC AACTGCTGCA CAACTTTCCT CCTGACCAGC TCACCAGCTC AGGGGCCCCT 
TTCTGGTCTG GACCCAAACG CTGTCCACAC CCACTTACTT TTGATGTTAA CAATACATTG 
CATCTGGATT ATGTGATGGC TGCTGCCAAC CTTTTTGCCC AGACCTATGG GTTGACTGGA 
TCCCAAGACC GAGCTGCTGT GGCCTCACTC CTGCAGTCAG TACAAGTCCC AGAGTTCACC 
CCCAAGTCTG GTGTCAAGAT TCATGTTTCT GACCAGGAGT TGCAGAGTGC CAATGCCTCT 
GTTGATGACA GCCGTCTTGA GGAGCTCAAA GCCACATTGC CCAGCCCAGA CAAGTTACCC 
GGATTTAAGA TGTACCCCAT TGATTTTGAG AAGGATGATG ACAGCAATTT CCACATGGAT 
TTCATTGTGG CTGCATCCAA TCTTCGGGCC GAAAACTATG ATATTTCCCC TGCAGACCGA 
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACAAC CACAGCTGCT 
GTGGTTGGCC TTGTGTGTCT GGAGCTCTAC AAGGTAGTTC AAGGGCACCA ACAGCTCGAT 
TCCTACAAAA ATGGTTTCCT GAACTTGGCC CTGCCCTTCT TTGGGTTTTC TGAACCTCTT 
GCTGCACCTC GTCACCAGTA CTATAATCAA GAGTGGACAT TGTGGGATCG CTTTGAAGTA 
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAGC AGTTCCTTGA TTACTTTAAG 
ACAGAGCACA AATTGGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCTTTC 
TTCATGCCAG CTGCTAAGCT CAAGGAACGA TTGGATCAGC CGATGACAGA GATTGTGAGC 
CGAGTGTCAA AGAGAAAGCT GGGCCGCCAT GTGCGGGCAC TGGTGCTTGA GCTGTGCTGC 
AACGATGAAA GCGGCGAGGA CGTCGAGGTC CCTTATGTCC GATATACCAT TCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Uba1 ( XM_030251302.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107162.1 Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15938
Clone ID Related Accession (Same CDS sequence)	NM_001276317.1 , XM_030251302.1 , NM_001276316.1 , NM_001136085.2
Accession Version	XM_030251302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin-like modifier-activating enzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGCT CCCCTGCACA GTCTGCGCTG TCCGAAGTGT CCTCAGTGCC AACCAACGGA 
ATGGCGAAGA ACGGCAGTGA AGCAGACATA GACGAGAGCC TTTACTCCCG GCAGCTGTAC 
GTTTTGGGCC ATGAGGCAAT GAAAATGCTC CAGACATCCA GCGTCCTTGT CTCAGGCTTG 
CGGGGCTTGG GTGTAGAAAT TGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTCACC 
CTACATGACC AAGGAACTAC CCAGTGGGCT GATCTCTCCT CCCAGTTTTA CCTTCGGGAG 
GAGGACATTG GTAAAAATCG AGCGGAGGTA TCCCAGCCCC GACTTGCTGA ACTCAACAGC 
TATGTACCTG TCACTGCCTA CACTGGGCCT CTTGTCGAGG ACTTCCTTAG TAGCTTCCAG 
GTGGTGGTCC TCACCAACAG CCCCCTGGAA GCCCAGCTGC GAGTGGGGGA GTTCTGTCAT 
AGCCGTGGTA TCAAGCTAGT GGTGGCAGAT ACAAGAGGCC TGTTTGGGCA ACTTTTCTGT 
GATTTTGGAG AGGAAATGGT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG 
GTTTCAATGG TCACCAAGGA CAACCCCGGT GTGGTTACCT GCCTTGATGA GGCCCGACAT 
GGCTTTGAGA CTGGTGACTT CGTCTCATTC TCAGAAGTAC AGGGCATGAT CCAACTCAAT 
GGATGTCAGC CCATGGAGAT CAAAGTGCTG GGTCCTTATA CCTTTAGTAT CTGTGACACT 
TCCAACTTCT CTGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCGAAAAAG 
ATTAGTTTTA AATCCTTGCC AGCATCACTG GTAGAGCCTG ACTTTGTGAT GACTGACTTT 
GCCAAGTATT CTCGCCCTGC CCAACTGCAC ATTGGCTTCC AAGCTCTGCA CCAATTCTGT 
GCTCTGCACA ACCAACCACC TCGACCACGA AATGAGGAAG ATGCAACAGA GCTGGTGGGC 
CTGGCTCAGG CTGTAAACGC TCGGTCCCCA CCTTCAGTAA AACAGAACAG CTTGGATGAA 
GACCTTATTC GGAAGCTAGC TTATGTTGCT GCTGGGGACC TGGCACCCAT AAATGCTTTC 
ATTGGGGGCC TTGCTGCCCA GGAAGTCATG AAGGCCTGCT CTGGAAAGTT TATGCCCATC 
ATGCAGTGGT TGTACTTTGA TGCTCTTGAA TGTCTCCCAG AGGACAAAGA GGCTCTGACA 
GAGGAGAAGT GCCTCCCACG TCAGAACCGT TACGATGGGC AGGTAGCTGT ATTTGGGTCA 
GACTTTCAGG AGAAGCTGAG CAAGCAAAAG TACTTCCTGG TGGGTGCAGG GGCCATTGGC 
TGTGAATTGC TCAAGAACTT TGCCATGATT GGGCTGGGTT GTGGAGAGGG TGGAGAGGTC 
GTGGTCACAG ACATGGACAC CATTGAGAAA TCAAATCTGA ACCGACAGTT TCTCTTCCGG 
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCCGCTG CAGCTGTGCG CCAGATGAAT 
CCTTACATCC AGGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA TTTGGATGGT GTGGCCAATG CTCTGGACAA CATAGATGCC 
CGCATGTACA TGGATCGCCG ATGTGTGTAC TATCGGAAGC CACTGCTAGA GTCTGGCACA 
CTGGGCACAA AGGGCAACGT GCAGGTGGTA ATCCCCTTCC TGACAGAATC CTACAGCTCT 
AGCCAGGATC CACCAGAGAA ATCCATCCCC ATTTGTACCC TGAAAAACTT TCCCAATGCC 
ATCGAACACA CTCTTCAGTG GGCCCGGGAT GAATTTGAAG GCCTTTTCAA GCAGCCAGCA 
GAAAATGTTA ATCAGTACCT CACAGACTCC AAATTTGTGG AGCGGACATT GCGGCTGGCT 
GGTACCCAGC CATTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGTT GCAGCGACCA 
CAGACTTGGG GAGACTGTGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTGTAAC 
AACATCCGGC AACTGCTGCA CAACTTTCCT CCTGACCAGC TCACCAGCTC AGGGGCCCCT 
TTCTGGTCTG GACCCAAACG CTGTCCACAC CCACTTACTT TTGATGTTAA CAATACATTG 
CATCTGGATT ATGTGATGGC TGCTGCCAAC CTTTTTGCCC AGACCTATGG GTTGACTGGA 
TCCCAAGACC GAGCTGCTGT GGCCTCACTC CTGCAGTCAG TACAAGTCCC AGAGTTCACC 
CCCAAGTCTG GTGTCAAGAT TCATGTTTCT GACCAGGAGT TGCAGAGTGC CAATGCCTCT 
GTTGATGACA GCCGTCTTGA GGAGCTCAAA GCCACATTGC CCAGCCCAGA CAAGTTACCC 
GGATTTAAGA TGTACCCCAT TGATTTTGAG AAGGATGATG ACAGCAATTT CCACATGGAT 
TTCATTGTGG CTGCATCCAA TCTTCGGGCC GAAAACTATG ATATTTCCCC TGCAGACCGA 
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACAAC CACAGCTGCT 
GTGGTTGGCC TTGTGTGTCT GGAGCTCTAC AAGGTAGTTC AAGGGCACCA ACAGCTCGAT 
TCCTACAAAA ATGGTTTCCT GAACTTGGCC CTGCCCTTCT TTGGGTTTTC TGAACCTCTT 
GCTGCACCTC GTCACCAGTA CTATAATCAA GAGTGGACAT TGTGGGATCG CTTTGAAGTA 
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAGC AGTTCCTTGA TTACTTTAAG 
ACAGAGCACA AATTGGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCTTTC 
TTCATGCCAG CTGCTAAGCT CAAGGAACGA TTGGATCAGC CGATGACAGA GATTGTGAGC 
CGAGTGTCAA AGAGAAAGCT GGGCCGCCAT GTGCGGGCAC TGGTGCTTGA GCTGTGCTGC 
AACGATGAAA GCGGCGAGGA CGTCGAGGTC CCTTATGTCC GATATACCAT TCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107162.1
CDS	794..3970
Translation	MSSSPLSKKRRVSGPDPKPGSNCSPAQSALSEVSSVPTNGMAKNGSEADIDESLYSRQLYVLGHEAMKMLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTTQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSSFQVVVLTNSPLEAQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMVLTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFETGDFVSFSEVQGMIQLNGCQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLVEPDFVMTDFAKYSRPAQLHIGFQALHQFCALHNQPPRPRNEEDATELVGLAQAVNARSPPSVKQNSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEEKCLPRQNRYDGQVAVFGSDFQEKLSKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEVVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPYIQVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNIDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDSKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWGDCVTWACHHWHTQYCNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNTLHLDYVMAAANLFAQTYGLTGSQDRAAVASLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	XM_030251302.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251302.1

ORF Insert Sequence:

ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGCT CCCCTGCACA GTCTGCGCTG TCCGAAGTGT CCTCAGTGCC AACCAACGGA 
ATGGCGAAGA ACGGCAGTGA AGCAGACATA GACGAGAGCC TTTACTCCCG GCAGCTGTAC 
GTTTTGGGCC ATGAGGCAAT GAAAATGCTC CAGACATCCA GCGTCCTTGT CTCAGGCTTG 
CGGGGCTTGG GTGTAGAAAT TGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTCACC 
CTACATGACC AAGGAACTAC CCAGTGGGCT GATCTCTCCT CCCAGTTTTA CCTTCGGGAG 
GAGGACATTG GTAAAAATCG AGCGGAGGTA TCCCAGCCCC GACTTGCTGA ACTCAACAGC 
TATGTACCTG TCACTGCCTA CACTGGGCCT CTTGTCGAGG ACTTCCTTAG TAGCTTCCAG 
GTGGTGGTCC TCACCAACAG CCCCCTGGAA GCCCAGCTGC GAGTGGGGGA GTTCTGTCAT 
AGCCGTGGTA TCAAGCTAGT GGTGGCAGAT ACAAGAGGCC TGTTTGGGCA ACTTTTCTGT 
GATTTTGGAG AGGAAATGGT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG 
GTTTCAATGG TCACCAAGGA CAACCCCGGT GTGGTTACCT GCCTTGATGA GGCCCGACAT 
GGCTTTGAGA CTGGTGACTT CGTCTCATTC TCAGAAGTAC AGGGCATGAT CCAACTCAAT 
GGATGTCAGC CCATGGAGAT CAAAGTGCTG GGTCCTTATA CCTTTAGTAT CTGTGACACT 
TCCAACTTCT CTGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCGAAAAAG 
ATTAGTTTTA AATCCTTGCC AGCATCACTG GTAGAGCCTG ACTTTGTGAT GACTGACTTT 
GCCAAGTATT CTCGCCCTGC CCAACTGCAC ATTGGCTTCC AAGCTCTGCA CCAATTCTGT 
GCTCTGCACA ACCAACCACC TCGACCACGA AATGAGGAAG ATGCAACAGA GCTGGTGGGC 
CTGGCTCAGG CTGTAAACGC TCGGTCCCCA CCTTCAGTAA AACAGAACAG CTTGGATGAA 
GACCTTATTC GGAAGCTAGC TTATGTTGCT GCTGGGGACC TGGCACCCAT AAATGCTTTC 
ATTGGGGGCC TTGCTGCCCA GGAAGTCATG AAGGCCTGCT CTGGAAAGTT TATGCCCATC 
ATGCAGTGGT TGTACTTTGA TGCTCTTGAA TGTCTCCCAG AGGACAAAGA GGCTCTGACA 
GAGGAGAAGT GCCTCCCACG TCAGAACCGT TACGATGGGC AGGTAGCTGT ATTTGGGTCA 
GACTTTCAGG AGAAGCTGAG CAAGCAAAAG TACTTCCTGG TGGGTGCAGG GGCCATTGGC 
TGTGAATTGC TCAAGAACTT TGCCATGATT GGGCTGGGTT GTGGAGAGGG TGGAGAGGTC 
GTGGTCACAG ACATGGACAC CATTGAGAAA TCAAATCTGA ACCGACAGTT TCTCTTCCGG 
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCCGCTG CAGCTGTGCG CCAGATGAAT 
CCTTACATCC AGGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA TTTGGATGGT GTGGCCAATG CTCTGGACAA CATAGATGCC 
CGCATGTACA TGGATCGCCG ATGTGTGTAC TATCGGAAGC CACTGCTAGA GTCTGGCACA 
CTGGGCACAA AGGGCAACGT GCAGGTGGTA ATCCCCTTCC TGACAGAATC CTACAGCTCT 
AGCCAGGATC CACCAGAGAA ATCCATCCCC ATTTGTACCC TGAAAAACTT TCCCAATGCC 
ATCGAACACA CTCTTCAGTG GGCCCGGGAT GAATTTGAAG GCCTTTTCAA GCAGCCAGCA 
GAAAATGTTA ATCAGTACCT CACAGACTCC AAATTTGTGG AGCGGACATT GCGGCTGGCT 
GGTACCCAGC CATTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGTT GCAGCGACCA 
CAGACTTGGG GAGACTGTGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTGTAAC 
AACATCCGGC AACTGCTGCA CAACTTTCCT CCTGACCAGC TCACCAGCTC AGGGGCCCCT 
TTCTGGTCTG GACCCAAACG CTGTCCACAC CCACTTACTT TTGATGTTAA CAATACATTG 
CATCTGGATT ATGTGATGGC TGCTGCCAAC CTTTTTGCCC AGACCTATGG GTTGACTGGA 
TCCCAAGACC GAGCTGCTGT GGCCTCACTC CTGCAGTCAG TACAAGTCCC AGAGTTCACC 
CCCAAGTCTG GTGTCAAGAT TCATGTTTCT GACCAGGAGT TGCAGAGTGC CAATGCCTCT 
GTTGATGACA GCCGTCTTGA GGAGCTCAAA GCCACATTGC CCAGCCCAGA CAAGTTACCC 
GGATTTAAGA TGTACCCCAT TGATTTTGAG AAGGATGATG ACAGCAATTT CCACATGGAT 
TTCATTGTGG CTGCATCCAA TCTTCGGGCC GAAAACTATG ATATTTCCCC TGCAGACCGA 
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACAAC CACAGCTGCT 
GTGGTTGGCC TTGTGTGTCT GGAGCTCTAC AAGGTAGTTC AAGGGCACCA ACAGCTCGAT 
TCCTACAAAA ATGGTTTCCT GAACTTGGCC CTGCCCTTCT TTGGGTTTTC TGAACCTCTT 
GCTGCACCTC GTCACCAGTA CTATAATCAA GAGTGGACAT TGTGGGATCG CTTTGAAGTA 
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAGC AGTTCCTTGA TTACTTTAAG 
ACAGAGCACA AATTGGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCTTTC 
TTCATGCCAG CTGCTAAGCT CAAGGAACGA TTGGATCAGC CGATGACAGA GATTGTGAGC 
CGAGTGTCAA AGAGAAAGCT GGGCCGCCAT GTGCGGGCAC TGGTGCTTGA GCTGTGCTGC 
AACGATGAAA GCGGCGAGGA CGTCGAGGTC CCTTATGTCC GATATACCAT TCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Uba1 ( NM_009457.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033483.2 Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 1, mRNA.

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CloneID	OMu04198
Clone ID Related Accession (Same CDS sequence)	NM_009457.4
Accession Version	NM_009457.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	ubiquitin-like modifier-activating enzyme 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY004827.1, BC145984.1 and AK143416.1. On Jan 29, 2013 this sequence version replaced NM_009457.3. Transcript Variant: This variant (1) uses an alternate 5' exon structure and an upstream start codon, compared to variant 3. The encoded isoform (1) has a longer N-terminus, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK088057.1, SRR1660815.163696.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTCT ATTCAGGCGT CTATGGTATA CCCCAGACCC GGCCCTCTAA GGCCCCCGCG 
CTTCCGAGCT CCGCGCAAAC TCTGGTTCTT TTTGCACGAT CGAGGTGGTT TGCTCTTCCG 
TTGCCCTGTG GCTTCGGCTT GTCTCCAGAA GGAAGGCGAG GCTTCCGCCC AGCACTGGGG 
ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGCT CCCCTGCACA GTCTGCGCTG TCCGAAGTGT CCTCAGTGCC AACCAACGGA 
ATGGCGAAGA ACGGCAGTGA AGCAGACATA GACGAGAGCC TTTACTCCCG GCAGCTGTAC 
GTTTTGGGCC ATGAGGCAAT GAAAATGCTC CAGACATCCA GCGTCCTTGT CTCAGGCTTG 
CGGGGCTTGG GTGTAGAAAT TGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTCACC 
CTACATGACC AAGGAACTAC CCAGTGGGCT GATCTCTCCT CCCAGTTTTA CCTTCGGGAG 
GAGGACATTG GTAAAAATCG AGCGGAGGTA TCCCAGCCCC GACTTGCTGA ACTCAACAGC 
TATGTACCTG TCACTGCCTA CACTGGGCCT CTTGTCGAGG ACTTCCTTAG TAGCTTCCAG 
GTGGTGGTCC TCACCAACAG CCCCCTGGAA GCCCAGCTGC GAGTGGGGGA GTTCTGTCAT 
AGCCGTGGTA TCAAGCTAGT GGTGGCAGAT ACAAGAGGCC TGTTTGGGCA ACTTTTCTGT 
GATTTTGGAG AGGAAATGGT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG 
GTTTCAATGG TCACCAAGGA CAACCCCGGT GTGGTTACCT GCCTTGATGA GGCCCGACAT 
GGCTTTGAGA CTGGTGACTT CGTCTCATTC TCAGAAGTAC AGGGCATGAT CCAACTCAAT 
GGATGTCAGC CCATGGAGAT CAAAGTGCTG GGTCCTTATA CCTTTAGTAT CTGTGACACT 
TCCAACTTCT CTGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCGAAAAAG 
ATTAGTTTTA AATCCTTGCC AGCATCACTG GTAGAGCCTG ACTTTGTGAT GACTGACTTT 
GCCAAGTATT CTCGCCCTGC CCAACTGCAC ATTGGCTTCC AAGCTCTGCA CCAATTCTGT 
GCTCTGCACA ACCAACCACC TCGACCACGA AATGAGGAAG ATGCAACAGA GCTGGTGGGC 
CTGGCTCAGG CTGTAAACGC TCGGTCCCCA CCTTCAGTAA AACAGAACAG CTTGGATGAA 
GACCTTATTC GGAAGCTAGC TTATGTTGCT GCTGGGGACC TGGCACCCAT AAATGCTTTC 
ATTGGGGGCC TTGCTGCCCA GGAAGTCATG AAGGCCTGCT CTGGAAAGTT TATGCCCATC 
ATGCAGTGGT TGTACTTTGA TGCTCTTGAA TGTCTCCCAG AGGACAAAGA GGCTCTGACA 
GAGGAGAAGT GCCTCCCACG TCAGAACCGT TACGATGGGC AGGTAGCTGT ATTTGGGTCA 
GACTTTCAGG AGAAGCTGAG CAAGCAAAAG TACTTCCTGG TGGGTGCAGG GGCCATTGGC 
TGTGAATTGC TCAAGAACTT TGCCATGATT GGGCTGGGTT GTGGAGAGGG TGGAGAGGTC 
GTGGTCACAG ACATGGACAC CATTGAGAAA TCAAATCTGA ACCGACAGTT TCTCTTCCGG 
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCCGCTG CAGCTGTGCG CCAGATGAAT 
CCTTACATCC AGGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA TTTGGATGGT GTGGCCAATG CTCTGGACAA CATAGATGCC 
CGCATGTACA TGGATCGCCG ATGTGTGTAC TATCGGAAGC CACTGCTAGA GTCTGGCACA 
CTGGGCACAA AGGGCAACGT GCAGGTGGTA ATCCCCTTCC TGACAGAATC CTACAGCTCT 
AGCCAGGATC CACCAGAGAA ATCCATCCCC ATTTGTACCC TGAAAAACTT TCCCAATGCC 
ATCGAACACA CTCTTCAGTG GGCCCGGGAT GAATTTGAAG GCCTTTTCAA GCAGCCAGCA 
GAAAATGTTA ATCAGTACCT CACAGACTCC AAATTTGTGG AGCGGACATT GCGGCTGGCT 
GGTACCCAGC CATTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGTT GCAGCGACCA 
CAGACTTGGG GAGACTGTGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTGTAAC 
AACATCCGGC AACTGCTGCA CAACTTTCCT CCTGACCAGC TCACCAGCTC AGGGGCCCCT 
TTCTGGTCTG GACCCAAACG CTGTCCACAC CCACTTACTT TTGATGTTAA CAATACATTG 
CATCTGGATT ATGTGATGGC TGCTGCCAAC CTTTTTGCCC AGACCTATGG GTTGACTGGA 
TCCCAAGACC GAGCTGCTGT GGCCTCACTC CTGCAGTCAG TACAAGTCCC AGAGTTCACC 
CCCAAGTCTG GTGTCAAGAT TCATGTTTCT GACCAGGAGT TGCAGAGTGC CAATGCCTCT 
GTTGATGACA GCCGTCTTGA GGAGCTCAAA GCCACATTGC CCAGCCCAGA CAAGTTACCC 
GGATTTAAGA TGTACCCCAT TGATTTTGAG AAGGATGATG ACAGCAATTT CCACATGGAT 
TTCATTGTGG CTGCATCCAA TCTTCGGGCC GAAAACTATG ATATTTCCCC TGCAGACCGA 
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACAAC CACAGCTGCT 
GTGGTTGGCC TTGTGTGTCT GGAGCTCTAC AAGGTAGTTC AAGGGCACCA ACAGCTCGAT 
TCCTACAAAA ATGGTTTCCT GAACTTGGCC CTGCCCTTCT TTGGGTTTTC TGAACCTCTT 
GCTGCACCTC GTCACCAGTA CTATAATCAA GAGTGGACAT TGTGGGATCG CTTTGAAGTA 
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAGC AGTTCCTTGA TTACTTTAAG 
ACAGAGCACA AATTGGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCTTTC 
TTCATGCCAG CTGCTAAGCT CAAGGAACGA TTGGATCAGC CGATGACAGA GATTGTGAGC 
CGAGTGTCAA AGAGAAAGCT GGGCCGCCAT GTGCGGGCAC TGGTGCTTGA GCTGTGCTGC 
AACGATGAAA GCGGCGAGGA CGTCGAGGTC CCTTATGTCC GATATACCAT TCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033483.2
CDS	46..3402
Translation	MPLYSGVYGIPQTRPSKAPALPSSAQTLVLFARSRWFALPLPCGFGLSPEGRRGFRPALGMSSSPLSKKRRVSGPDPKPGSNCSPAQSALSEVSSVPTNGMAKNGSEADIDESLYSRQLYVLGHEAMKMLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTTQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSSFQVVVLTNSPLEAQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMVLTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFETGDFVSFSEVQGMIQLNGCQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLVEPDFVMTDFAKYSRPAQLHIGFQALHQFCALHNQPPRPRNEEDATELVGLAQAVNARSPPSVKQNSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEEKCLPRQNRYDGQVAVFGSDFQEKLSKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEVVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPYIQVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNIDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDSKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWGDCVTWACHHWHTQYCNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNTLHLDYVMAAANLFAQTYGLTGSQDRAAVASLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Target ORF information:

RefSeq Version	NM_009457.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009457.4

ORF Insert Sequence:

ATGCCCCTCT ATTCAGGCGT CTATGGTATA CCCCAGACCC GGCCCTCTAA GGCCCCCGCG 
CTTCCGAGCT CCGCGCAAAC TCTGGTTCTT TTTGCACGAT CGAGGTGGTT TGCTCTTCCG 
TTGCCCTGTG GCTTCGGCTT GTCTCCAGAA GGAAGGCGAG GCTTCCGCCC AGCACTGGGG 
ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT 
TCTAACTGCT CCCCTGCACA GTCTGCGCTG TCCGAAGTGT CCTCAGTGCC AACCAACGGA 
ATGGCGAAGA ACGGCAGTGA AGCAGACATA GACGAGAGCC TTTACTCCCG GCAGCTGTAC 
GTTTTGGGCC ATGAGGCAAT GAAAATGCTC CAGACATCCA GCGTCCTTGT CTCAGGCTTG 
CGGGGCTTGG GTGTAGAAAT TGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTCACC 
CTACATGACC AAGGAACTAC CCAGTGGGCT GATCTCTCCT CCCAGTTTTA CCTTCGGGAG 
GAGGACATTG GTAAAAATCG AGCGGAGGTA TCCCAGCCCC GACTTGCTGA ACTCAACAGC 
TATGTACCTG TCACTGCCTA CACTGGGCCT CTTGTCGAGG ACTTCCTTAG TAGCTTCCAG 
GTGGTGGTCC TCACCAACAG CCCCCTGGAA GCCCAGCTGC GAGTGGGGGA GTTCTGTCAT 
AGCCGTGGTA TCAAGCTAGT GGTGGCAGAT ACAAGAGGCC TGTTTGGGCA ACTTTTCTGT 
GATTTTGGAG AGGAAATGGT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG 
GTTTCAATGG TCACCAAGGA CAACCCCGGT GTGGTTACCT GCCTTGATGA GGCCCGACAT 
GGCTTTGAGA CTGGTGACTT CGTCTCATTC TCAGAAGTAC AGGGCATGAT CCAACTCAAT 
GGATGTCAGC CCATGGAGAT CAAAGTGCTG GGTCCTTATA CCTTTAGTAT CTGTGACACT 
TCCAACTTCT CTGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCGAAAAAG 
ATTAGTTTTA AATCCTTGCC AGCATCACTG GTAGAGCCTG ACTTTGTGAT GACTGACTTT 
GCCAAGTATT CTCGCCCTGC CCAACTGCAC ATTGGCTTCC AAGCTCTGCA CCAATTCTGT 
GCTCTGCACA ACCAACCACC TCGACCACGA AATGAGGAAG ATGCAACAGA GCTGGTGGGC 
CTGGCTCAGG CTGTAAACGC TCGGTCCCCA CCTTCAGTAA AACAGAACAG CTTGGATGAA 
GACCTTATTC GGAAGCTAGC TTATGTTGCT GCTGGGGACC TGGCACCCAT AAATGCTTTC 
ATTGGGGGCC TTGCTGCCCA GGAAGTCATG AAGGCCTGCT CTGGAAAGTT TATGCCCATC 
ATGCAGTGGT TGTACTTTGA TGCTCTTGAA TGTCTCCCAG AGGACAAAGA GGCTCTGACA 
GAGGAGAAGT GCCTCCCACG TCAGAACCGT TACGATGGGC AGGTAGCTGT ATTTGGGTCA 
GACTTTCAGG AGAAGCTGAG CAAGCAAAAG TACTTCCTGG TGGGTGCAGG GGCCATTGGC 
TGTGAATTGC TCAAGAACTT TGCCATGATT GGGCTGGGTT GTGGAGAGGG TGGAGAGGTC 
GTGGTCACAG ACATGGACAC CATTGAGAAA TCAAATCTGA ACCGACAGTT TCTCTTCCGG 
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCCGCTG CAGCTGTGCG CCAGATGAAT 
CCTTACATCC AGGTGACAAG CCACCAGAAC CGTGTAGGTC CTGACACTGA GCGCATCTAT 
GATGATGATT TCTTCCAAAA TTTGGATGGT GTGGCCAATG CTCTGGACAA CATAGATGCC 
CGCATGTACA TGGATCGCCG ATGTGTGTAC TATCGGAAGC CACTGCTAGA GTCTGGCACA 
CTGGGCACAA AGGGCAACGT GCAGGTGGTA ATCCCCTTCC TGACAGAATC CTACAGCTCT 
AGCCAGGATC CACCAGAGAA ATCCATCCCC ATTTGTACCC TGAAAAACTT TCCCAATGCC 
ATCGAACACA CTCTTCAGTG GGCCCGGGAT GAATTTGAAG GCCTTTTCAA GCAGCCAGCA 
GAAAATGTTA ATCAGTACCT CACAGACTCC AAATTTGTGG AGCGGACATT GCGGCTGGCT 
GGTACCCAGC CATTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGTT GCAGCGACCA 
CAGACTTGGG GAGACTGTGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTGTAAC 
AACATCCGGC AACTGCTGCA CAACTTTCCT CCTGACCAGC TCACCAGCTC AGGGGCCCCT 
TTCTGGTCTG GACCCAAACG CTGTCCACAC CCACTTACTT TTGATGTTAA CAATACATTG 
CATCTGGATT ATGTGATGGC TGCTGCCAAC CTTTTTGCCC AGACCTATGG GTTGACTGGA 
TCCCAAGACC GAGCTGCTGT GGCCTCACTC CTGCAGTCAG TACAAGTCCC AGAGTTCACC 
CCCAAGTCTG GTGTCAAGAT TCATGTTTCT GACCAGGAGT TGCAGAGTGC CAATGCCTCT 
GTTGATGACA GCCGTCTTGA GGAGCTCAAA GCCACATTGC CCAGCCCAGA CAAGTTACCC 
GGATTTAAGA TGTACCCCAT TGATTTTGAG AAGGATGATG ACAGCAATTT CCACATGGAT 
TTCATTGTGG CTGCATCCAA TCTTCGGGCC GAAAACTATG ATATTTCCCC TGCAGACCGA 
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACAAC CACAGCTGCT 
GTGGTTGGCC TTGTGTGTCT GGAGCTCTAC AAGGTAGTTC AAGGGCACCA ACAGCTCGAT 
TCCTACAAAA ATGGTTTCCT GAACTTGGCC CTGCCCTTCT TTGGGTTTTC TGAACCTCTT 
GCTGCACCTC GTCACCAGTA CTATAATCAA GAGTGGACAT TGTGGGATCG CTTTGAAGTA 
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAGC AGTTCCTTGA TTACTTTAAG 
ACAGAGCACA AATTGGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCTTTC 
TTCATGCCAG CTGCTAAGCT CAAGGAACGA TTGGATCAGC CGATGACAGA GATTGTGAGC 
CGAGTGTCAA AGAGAAAGCT GGGCCGCCAT GTGCGGGCAC TGGTGCTTGA GCTGTGCTGC 
AACGATGAAA GCGGCGAGGA CGTCGAGGTC CCTTATGTCC GATATACCAT TCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread and dynamic translational control of red blood cell development.
Blood129(5)619-629(2017 02)
Alvarez-Dominguez JR,Zhang X,Hu W

Ubiquitin-activating enzyme activity contributes to differential accumulation of mutant huntingtin in brain and peripheral tissues.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(25)8411-22(2014 Jun)
Wade BE,Wang CE,Yan S,Bhat K,Huang B,Li S,Li XJ

Ubiquitin-activating enzyme E1 inhibitor PYR41 attenuates angiotensin II-induced activation of dendritic cells via the I?Ba/NF-?B and MKP1/ERK/STAT1 pathways.
Immunology142(2)307-19(2014 Jun)
Chen C,Meng Y,Wang L,Wang HX,Tian C,Pang GD,Li HH,Du J

Dysregulation of ubiquitin homeostasis and ?-catenin signaling promote spinal muscular atrophy.
The Journal of clinical investigation124(4)1821-34(2014 Apr)
Wishart TM,Mutsaers CA,Riessland M,Reimer MM,Hunter G,Hannam ML,Eaton SL,Fuller HR,Roche SL,Somers E,Morse R,Young PJ,Lamont DJ,Hammerschmidt M,Joshi A,Hohenstein P,Morris GE,Parson SH,Skehel PA,Becker T,Robinson IM,Becker CG,Wirth B,Gillingwater TH

Epigenetic functions of smchd1 repress gene clusters on the inactive X chromosome and on autosomes.
Molecular and cellular biology33(16)3150-65(2013 Aug)
Gendrel AV,Tang YA,Suzuki M,Godwin J,Nesterova TB,Greally JM,Heard E,Brockdorff N

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Uba1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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