Top2a cDNA ORF clone, Mus musculus(house mouse)

The following Top2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu20097 XM_006533153.3
Latest version!
Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu46211 XM_006533154.3
Latest version!
Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu46211 XM_006533155.3
Latest version!
Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu20097 NM_011623.2
Latest version!
Mus musculus topoisomerase (DNA) II alpha (Top2a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
View Old Accession Versions >>
OMu46211 XM_006533155.2 Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu46211 XM_006533154.2 Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu20097 XM_006533153.2 Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu20097
    Clone ID Related Accession (Same CDS sequence) NM_011623.2 , XM_006533153.3 , XM_006533153.2
    Accession Version XM_006533153.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product DNA topoisomerase 2-alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533153.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
    GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA
    CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG
    TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG
    TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA
    ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT
    GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA
    TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT
    CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA
    GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT
    GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT
    GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA
    AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT
    CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA
    ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA
    ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG
    GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG
    AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG
    ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG
    ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT
    GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC
    CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT
    GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG
    GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT
    GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC
    ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC
    TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT
    CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG
    CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC
    AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG
    ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA
    AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT
    GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA
    AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT
    GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG
    GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT
    TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA
    GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG
    ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG
    AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT
    AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA
    GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC
    ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC
    TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT
    GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA
    CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT
    GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA
    CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT
    ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG
    AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG
    CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC
    TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT
    GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC
    AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC
    TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA
    GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG
    ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG
    CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA
    TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC
    AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG
    AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA
    GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA
    AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA
    GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT
    GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT
    GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC
    AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG
    GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT
    ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TAGTTGATCT TGAAAGTGAT
    GTTAAGGACA GTGTGCCAGC TTCTCCAGGC GTTCCTGCTG CTGACTTCCC AGCGGAAACT
    GAACAGTCAA AGCCATCCAA AAAGACCGTG GGAGTGAAGA AGACAGCAAC CAAAAGCCAG
    TCTTCAGTCT CCACTGCTGG TACCAAAAAG AGAGCTGCGC CAAAGGGAAC CAAATCAGAT
    TCAGCCTTGA GTGCTCGTGT CTCGGAAAAA CCTGCTCCTG CCAAAGCCAA GAACAGTCGC
    AAAAGGAAGC CATCTTCTTC TGATAGCTCT GACTCTGATT TTGAGAGAGC AATTTCTAAA
    GGTGCCACGA GCAAGAAAGC AAAGGGAGAG GAACAGGACT TCCCTGTGGA CTTGGAAGAC
    ACGATAGCTC CTCGAGCCAA ATCTGACCGG GCGAGGAAGC CAATTAAGTA CCTGGAGGAG
    TCCGATGACG ATGACGACCT CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006533216.1
    CDS134..4720
    Translation

    Target ORF information:

    RefSeq Version XM_006533153.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006533153.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
    GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA
    CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG
    TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG
    TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA
    ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT
    GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA
    TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT
    CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA
    GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT
    GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT
    GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA
    AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT
    CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA
    ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA
    ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG
    GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG
    AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG
    ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG
    ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT
    GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC
    CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT
    GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG
    GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT
    GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC
    ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC
    TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT
    CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG
    CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC
    AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG
    ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA
    AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT
    GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA
    AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT
    GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG
    GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT
    TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA
    GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG
    ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG
    AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT
    AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA
    GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC
    ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC
    TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT
    GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA
    CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT
    GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA
    CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT
    ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG
    AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG
    CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC
    TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT
    GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC
    AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC
    TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA
    GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG
    ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG
    CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA
    TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC
    AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG
    AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA
    GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA
    AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA
    GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT
    GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT
    GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC
    AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG
    GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT
    ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TAGTTGATCT TGAAAGTGAT
    GTTAAGGACA GTGTGCCAGC TTCTCCAGGC GTTCCTGCTG CTGACTTCCC AGCGGAAACT
    GAACAGTCAA AGCCATCCAA AAAGACCGTG GGAGTGAAGA AGACAGCAAC CAAAAGCCAG
    TCTTCAGTCT CCACTGCTGG TACCAAAAAG AGAGCTGCGC CAAAGGGAAC CAAATCAGAT
    TCAGCCTTGA GTGCTCGTGT CTCGGAAAAA CCTGCTCCTG CCAAAGCCAA GAACAGTCGC
    AAAAGGAAGC CATCTTCTTC TGATAGCTCT GACTCTGATT TTGAGAGAGC AATTTCTAAA
    GGTGCCACGA GCAAGAAAGC AAAGGGAGAG GAACAGGACT TCCCTGTGGA CTTGGAAGAC
    ACGATAGCTC CTCGAGCCAA ATCTGACCGG GCGAGGAAGC CAATTAAGTA CCTGGAGGAG
    TCCGATGACG ATGACGACCT CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu46211
    Clone ID Related Accession (Same CDS sequence) XM_006533155.2 , XM_006533154.3 , XM_006533155.3 , XM_006533154.2
    Accession Version XM_006533154.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product DNA topoisomerase 2-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533154.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
    GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA
    CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG
    TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG
    TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA
    ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT
    GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA
    TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT
    CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA
    GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT
    GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT
    GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA
    AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT
    CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA
    ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA
    ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG
    GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG
    AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG
    ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG
    ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT
    GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC
    CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT
    GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG
    GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT
    GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC
    ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC
    TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT
    CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG
    CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC
    AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG
    ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA
    AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT
    GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA
    AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT
    GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG
    GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT
    TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA
    GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG
    ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG
    AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT
    AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA
    GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC
    ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC
    TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT
    GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA
    CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT
    GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA
    CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT
    ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG
    AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG
    CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC
    TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT
    GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC
    AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC
    TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA
    GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG
    ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG
    CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA
    TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC
    AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG
    AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA
    GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA
    AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA
    GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT
    GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT
    GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC
    AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG
    GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT
    ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT
    AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA
    CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT
    TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA
    GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA
    AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT
    GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG
    ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC
    GATGACGATG ACGACCTCTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006533217.1
    CDS134..4717
    Translation

    Target ORF information:

    RefSeq Version XM_006533154.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006533154.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
    GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA
    CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG
    TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG
    TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA
    ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT
    GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA
    TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT
    CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA
    GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT
    GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT
    GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA
    AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT
    CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA
    ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA
    ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG
    GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG
    AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG
    ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG
    ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT
    GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC
    CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT
    GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG
    GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT
    GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC
    ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC
    TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT
    CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG
    CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC
    AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG
    ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA
    AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT
    GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA
    AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT
    GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG
    GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT
    TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA
    GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG
    ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG
    AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT
    AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA
    GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC
    ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC
    TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT
    GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA
    CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT
    GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA
    CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT
    ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG
    AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG
    CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC
    TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT
    GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC
    AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC
    TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA
    GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG
    ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG
    CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA
    TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC
    AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG
    AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA
    GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA
    AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA
    GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT
    GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT
    GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC
    AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG
    GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT
    ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT
    AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA
    CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT
    TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA
    GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA
    AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT
    GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG
    ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC
    GATGACGATG ACGACCTCTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu46211
    Clone ID Related Accession (Same CDS sequence) XM_006533155.2 , XM_006533154.3 , XM_006533155.3 , XM_006533154.2
    Accession Version XM_006533155.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product DNA topoisomerase 2-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533155.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
    GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA
    CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG
    TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG
    TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA
    ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT
    GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA
    TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT
    CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA
    GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT
    GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT
    GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA
    AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT
    CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA
    ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA
    ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG
    GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG
    AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG
    ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG
    ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT
    GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC
    CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT
    GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG
    GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT
    GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC
    ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC
    TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT
    CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG
    CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC
    AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG
    ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA
    AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT
    GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA
    AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT
    GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG
    GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT
    TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA
    GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG
    ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG
    AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT
    AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA
    GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC
    ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC
    TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT
    GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA
    CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT
    GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA
    CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT
    ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG
    AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG
    CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC
    TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT
    GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC
    AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC
    TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA
    GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG
    ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG
    CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA
    TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC
    AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG
    AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA
    GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA
    AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA
    GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT
    GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT
    GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC
    AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG
    GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT
    ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT
    AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA
    CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT
    TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA
    GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA
    AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT
    GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG
    ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC
    GATGACGATG ACGACCTCTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006533218.1
    CDS134..4717
    Translation

    Target ORF information:

    RefSeq Version XM_006533155.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006533155.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
    GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA
    CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG
    TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG
    TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA
    ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT
    GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA
    TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT
    CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA
    GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT
    GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT
    GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA
    AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT
    CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA
    ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA
    ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG
    GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG
    AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG
    ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG
    ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT
    GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC
    CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT
    GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG
    GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT
    GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC
    ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC
    TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT
    CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG
    CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC
    AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG
    ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA
    AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT
    GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA
    AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT
    GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG
    GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT
    TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA
    GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG
    ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG
    AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT
    AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA
    GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC
    ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC
    TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT
    GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA
    CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT
    GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA
    CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT
    ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG
    AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG
    CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC
    TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT
    GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC
    AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC
    TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA
    GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG
    ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG
    CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA
    TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC
    AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG
    AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA
    GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA
    AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA
    GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT
    GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT
    GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC
    AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG
    GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT
    ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT
    AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA
    CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT
    TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA
    GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA
    AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT
    GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG
    ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC
    GATGACGATG ACGACCTCTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20097
    Clone ID Related Accession (Same CDS sequence) NM_011623.2 , XM_006533153.3 , XM_006533153.2
    Accession Version NM_011623.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-19
    Organism Mus musculus(house mouse)
    Product DNA topoisomerase 2-alpha
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591067.35. On Jul 26, 2007 this sequence version replaced NM_011623.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D12513.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
    GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA
    CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG
    TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG
    TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA
    ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT
    GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA
    TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT
    CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA
    GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT
    GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT
    GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA
    AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT
    CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA
    ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA
    ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG
    GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG
    AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG
    ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG
    ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT
    GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC
    CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT
    GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG
    GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT
    GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC
    ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC
    TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT
    CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG
    CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC
    AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG
    ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA
    AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT
    GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA
    AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT
    GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG
    GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT
    TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA
    GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG
    ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG
    AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT
    AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA
    GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC
    ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC
    TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT
    GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA
    CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT
    GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA
    CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT
    ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG
    AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG
    CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC
    TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT
    GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC
    AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC
    TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA
    GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG
    ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG
    CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA
    TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC
    AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG
    AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA
    GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA
    AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA
    GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT
    GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT
    GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC
    AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG
    GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT
    ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TAGTTGATCT TGAAAGTGAT
    GTTAAGGACA GTGTGCCAGC TTCTCCAGGC GTTCCTGCTG CTGACTTCCC AGCGGAAACT
    GAACAGTCAA AGCCATCCAA AAAGACCGTG GGAGTGAAGA AGACAGCAAC CAAAAGCCAG
    TCTTCAGTCT CCACTGCTGG TACCAAAAAG AGAGCTGCGC CAAAGGGAAC CAAATCAGAT
    TCAGCCTTGA GTGCTCGTGT CTCGGAAAAA CCTGCTCCTG CCAAAGCCAA GAACAGTCGC
    AAAAGGAAGC CATCTTCTTC TGATAGCTCT GACTCTGATT TTGAGAGAGC AATTTCTAAA
    GGTGCCACGA GCAAGAAAGC AAAGGGAGAG GAACAGGACT TCCCTGTGGA CTTGGAAGAC
    ACGATAGCTC CTCGAGCCAA ATCTGACCGG GCGAGGAAGC CAATTAAGTA CCTGGAGGAG
    TCCGATGACG ATGACGACCT CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_035753.2
    CDS124..4710
    Translation

    Target ORF information:

    RefSeq Version NM_011623.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus topoisomerase (DNA) II alpha (Top2a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_011623.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
    GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA
    CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG
    TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG
    TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA
    ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT
    GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA
    TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT
    CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA
    GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT
    GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT
    GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA
    AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT
    CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA
    ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA
    ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG
    GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG
    AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG
    ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG
    ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT
    GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC
    CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT
    GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG
    GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT
    GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC
    ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC
    TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT
    CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG
    CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC
    AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG
    ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA
    AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT
    GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA
    AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT
    GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG
    GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT
    TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA
    GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG
    ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG
    AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT
    AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA
    GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC
    ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC
    TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT
    GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA
    CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT
    GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA
    CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT
    ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG
    AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG
    CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC
    TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT
    GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC
    AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC
    TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA
    GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG
    ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG
    CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA
    TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC
    AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG
    AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA
    GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA
    AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA
    GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT
    GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT
    GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC
    AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG
    GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT
    ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TAGTTGATCT TGAAAGTGAT
    GTTAAGGACA GTGTGCCAGC TTCTCCAGGC GTTCCTGCTG CTGACTTCCC AGCGGAAACT
    GAACAGTCAA AGCCATCCAA AAAGACCGTG GGAGTGAAGA AGACAGCAAC CAAAAGCCAG
    TCTTCAGTCT CCACTGCTGG TACCAAAAAG AGAGCTGCGC CAAAGGGAAC CAAATCAGAT
    TCAGCCTTGA GTGCTCGTGT CTCGGAAAAA CCTGCTCCTG CCAAAGCCAA GAACAGTCGC
    AAAAGGAAGC CATCTTCTTC TGATAGCTCT GACTCTGATT TTGAGAGAGC AATTTCTAAA
    GGTGCCACGA GCAAGAAAGC AAAGGGAGAG GAACAGGACT TCCCTGTGGA CTTGGAAGAC
    ACGATAGCTC CTCGAGCCAA ATCTGACCGG GCGAGGAAGC CAATTAAGTA CCTGGAGGAG
    TCCGATGACG ATGACGACCT CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20097
    Clone ID Related Accession (Same CDS sequence) NM_011623.2 , XM_006533153.3 , XM_006533153.2
    Accession Version XM_006533153.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product DNA topoisomerase 2-alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533153.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
    GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA
    CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG
    TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG
    TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA
    ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT
    GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA
    TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT
    CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA
    GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT
    GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT
    GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA
    AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT
    CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA
    ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA
    ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG
    GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG
    AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG
    ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG
    ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT
    GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC
    CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT
    GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG
    GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT
    GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC
    ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC
    TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT
    CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG
    CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC
    AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG
    ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA
    AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT
    GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA
    AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT
    GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG
    GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT
    TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA
    GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG
    ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG
    AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT
    AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA
    GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC
    ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC
    TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT
    GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA
    CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT
    GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA
    CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT
    ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG
    AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG
    CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC
    TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT
    GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC
    AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC
    TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA
    GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG
    ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG
    CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA
    TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC
    AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG
    AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA
    GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA
    AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA
    GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT
    GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT
    GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC
    AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG
    GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT
    ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TAGTTGATCT TGAAAGTGAT
    GTTAAGGACA GTGTGCCAGC TTCTCCAGGC GTTCCTGCTG CTGACTTCCC AGCGGAAACT
    GAACAGTCAA AGCCATCCAA AAAGACCGTG GGAGTGAAGA AGACAGCAAC CAAAAGCCAG
    TCTTCAGTCT CCACTGCTGG TACCAAAAAG AGAGCTGCGC CAAAGGGAAC CAAATCAGAT
    TCAGCCTTGA GTGCTCGTGT CTCGGAAAAA CCTGCTCCTG CCAAAGCCAA GAACAGTCGC
    AAAAGGAAGC CATCTTCTTC TGATAGCTCT GACTCTGATT TTGAGAGAGC AATTTCTAAA
    GGTGCCACGA GCAAGAAAGC AAAGGGAGAG GAACAGGACT TCCCTGTGGA CTTGGAAGAC
    ACGATAGCTC CTCGAGCCAA ATCTGACCGG GCGAGGAAGC CAATTAAGTA CCTGGAGGAG
    TCCGATGACG ATGACGACCT CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006533216.1
    CDS140..4726
    Translation

    Target ORF information:

    RefSeq Version XM_006533153.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006533153.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
    GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA
    CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG
    TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG
    TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA
    ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT
    GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA
    TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT
    CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA
    GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT
    GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT
    GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA
    AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT
    CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA
    ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA
    ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG
    GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG
    AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG
    ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG
    ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT
    GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC
    CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT
    GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG
    GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT
    GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC
    ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC
    TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT
    CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG
    CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC
    AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG
    ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA
    AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT
    GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA
    AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT
    GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG
    GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT
    TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA
    GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG
    ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG
    AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT
    AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA
    GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC
    ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC
    TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT
    GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA
    CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT
    GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA
    CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT
    ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG
    AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG
    CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC
    TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT
    GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC
    AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC
    TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA
    GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG
    ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG
    CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA
    TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC
    AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG
    AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA
    GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA
    AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA
    GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT
    GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT
    GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC
    AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG
    GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT
    ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TAGTTGATCT TGAAAGTGAT
    GTTAAGGACA GTGTGCCAGC TTCTCCAGGC GTTCCTGCTG CTGACTTCCC AGCGGAAACT
    GAACAGTCAA AGCCATCCAA AAAGACCGTG GGAGTGAAGA AGACAGCAAC CAAAAGCCAG
    TCTTCAGTCT CCACTGCTGG TACCAAAAAG AGAGCTGCGC CAAAGGGAAC CAAATCAGAT
    TCAGCCTTGA GTGCTCGTGT CTCGGAAAAA CCTGCTCCTG CCAAAGCCAA GAACAGTCGC
    AAAAGGAAGC CATCTTCTTC TGATAGCTCT GACTCTGATT TTGAGAGAGC AATTTCTAAA
    GGTGCCACGA GCAAGAAAGC AAAGGGAGAG GAACAGGACT TCCCTGTGGA CTTGGAAGAC
    ACGATAGCTC CTCGAGCCAA ATCTGACCGG GCGAGGAAGC CAATTAAGTA CCTGGAGGAG
    TCCGATGACG ATGACGACCT CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu46211
    Clone ID Related Accession (Same CDS sequence) XM_006533155.2 , XM_006533154.3 , XM_006533155.3 , XM_006533154.2
    Accession Version XM_006533155.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product DNA topoisomerase 2-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533155.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
    GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA
    CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG
    TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG
    TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA
    ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT
    GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA
    TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT
    CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA
    GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT
    GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT
    GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA
    AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT
    CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA
    ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA
    ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG
    GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG
    AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG
    ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG
    ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT
    GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC
    CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT
    GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG
    GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT
    GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC
    ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC
    TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT
    CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG
    CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC
    AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG
    ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA
    AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT
    GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA
    AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT
    GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG
    GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT
    TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA
    GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG
    ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG
    AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT
    AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA
    GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC
    ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC
    TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT
    GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA
    CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT
    GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA
    CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT
    ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG
    AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG
    CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC
    TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT
    GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC
    AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC
    TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA
    GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG
    ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG
    CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA
    TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC
    AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG
    AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA
    GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA
    AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA
    GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT
    GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT
    GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC
    AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG
    GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT
    ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT
    AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA
    CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT
    TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA
    GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA
    AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT
    GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG
    ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC
    GATGACGATG ACGACCTCTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006533218.1
    CDS140..4723
    Translation

    Target ORF information:

    RefSeq Version XM_006533155.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006533155.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
    GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA
    CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG
    TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG
    TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA
    ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT
    GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA
    TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT
    CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA
    GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT
    GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT
    GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA
    AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT
    CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA
    ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA
    ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG
    GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG
    AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG
    ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG
    ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT
    GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC
    CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT
    GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG
    GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT
    GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC
    ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC
    TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT
    CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG
    CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC
    AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG
    ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA
    AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT
    GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA
    AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT
    GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG
    GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT
    TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA
    GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG
    ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG
    AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT
    AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA
    GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC
    ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC
    TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT
    GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA
    CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT
    GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA
    CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT
    ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG
    AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG
    CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC
    TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT
    GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC
    AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC
    TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA
    GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG
    ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG
    CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA
    TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC
    AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG
    AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA
    GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA
    AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA
    GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT
    GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT
    GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC
    AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG
    GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT
    ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT
    AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA
    CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT
    TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA
    GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA
    AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT
    GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG
    ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC
    GATGACGATG ACGACCTCTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu46211
    Clone ID Related Accession (Same CDS sequence) XM_006533155.2 , XM_006533154.3 , XM_006533155.3 , XM_006533154.2
    Accession Version XM_006533154.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product DNA topoisomerase 2-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533154.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
    GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA
    CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG
    TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG
    TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA
    ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT
    GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA
    TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT
    CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA
    GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT
    GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT
    GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA
    AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT
    CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA
    ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA
    ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG
    GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG
    AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG
    ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG
    ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT
    GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC
    CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT
    GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG
    GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT
    GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC
    ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC
    TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT
    CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG
    CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC
    AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG
    ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA
    AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT
    GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA
    AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT
    GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG
    GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT
    TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA
    GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG
    ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG
    AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT
    AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA
    GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC
    ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC
    TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT
    GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA
    CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT
    GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA
    CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT
    ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG
    AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG
    CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC
    TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT
    GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC
    AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC
    TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA
    GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG
    ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG
    CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA
    TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC
    AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG
    AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA
    GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA
    AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA
    GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT
    GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT
    GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC
    AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG
    GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT
    ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT
    AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA
    CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT
    TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA
    GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA
    AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT
    GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG
    ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC
    GATGACGATG ACGACCTCTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006533217.1
    CDS140..4723
    Translation

    Target ORF information:

    RefSeq Version XM_006533154.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus topoisomerase (DNA) II alpha (Top2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006533154.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGTTGT CACCGCTGCA GCCTGTAAAT GAAAATATGC TAATGAACAA AAAGAAGAAT 
    GAAGATGGCA AGAAAAGATT GTCTATTGAA AGAATCTATC AGAAAAAAAC ACAACTGGAA
    CATATACTGC TCCGCCCAGA TACCTACATT GGCTCTGTGG AGTTAGTGAC CCAGCAAATG
    TGGGTTTACG ATGAAGATGT TGGCATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG
    TATAAAATCT TTGATGAGAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA
    ATGTCTTGTA TTAGAGTCAC AATTGATCCA GAAAATAATG TAATTAGCAT CTGGAATAAT
    GGGAAAGGAA TCCCTGTTGT TGAACACAAA GTTGAGAAAA TATATGTCCC AGCTCTCATA
    TTTGGACAAC TCCTGACCTC CAGTAACTAT GACGACGATG AGAAGAAAGT GACAGGTGGT
    CGAAATGGCT ATGGAGCTAA ACTGTGTAAC ATATTCAGCA CCAAATTTAC TGTGGAAACA
    GCCAGTAGAG AGTACAAGAA AATGTTCAAA CAGACGTGGA TGGATAACAT GGGGAGAGCT
    GGTGACATGG AACTCAAGCC CTTTAGTGGA GAAGATTATA CATGTATCAC CTTCCAGCCT
    GACTTATCTA AGTTTAAAAT GCAAAGCCTG GACAAAGATA TTGTTGCACT GATGGTCAGA
    AGAGCATATG ATATCGCTGG CTCCACTAAA GATGTCAAAG TCTTTCTTAA TGGAAATAGT
    CTGCCAGTGA AAGGATTCCG CAGTTACGTG GATTTGTATT TGAAGGATAA GGTAGATGAA
    ACTGGGAATT CACTGAAGGT GATACATGAA CAGGTCAATC CCCGGTGGGA AGTGTGCTTA
    ACAATGAGCG AGAGAGGCTT CCAGCAGATT AGCTTCGTCA ACAGCATTGC GACTTCTAAG
    GGCGGCAGAC ATGTCGATTA TGTAGCTGAT CAGATTGTGA GCAAACTCGT TGATGTGGTG
    AAAAAGAAGA ACAAGGGCGG GGTTGCAGTG AAGGCTCATC AGGTGAAAAA TCACATGTGG
    ATTTTTGTAA ATGCCTTAAT TGAAAACCCA ACCTTTGACT CTCAAACAAA AGAAAACATG
    ACTTTACAAG CCAAGAGCTT TGGATCAACA TGTCAATTAA GTGAAAAGTT CATCAAAGCT
    GCAATTGGTT GTGGTATTGT GGAAAGCATA CTAAACTGGG TGAAGTTCAA GGCTCAAATC
    CAACTGAACA AGAAGTGTTC AGCTGTAAAA CATACCAAAA TCAAGGGAAT CCCCAAACTT
    GATGATGCCA ACGATGCAGG GAGCCGAAAC TCTACTGAAT GCACACTTAT CCTGACTGAG
    GGAGACTCAG CAAAAACTCT AGCCGTTTCA GGCCTTGGAG TGGTTGGAAG AGACAAATAT
    GGGGTGTTCC CTCTTAGAGG AAAGATACTC AATGTGCGAG AAGCCTCTCA TAAACAGATC
    ATGGAAAATG CTGAAATTAA CAATATCATC AAGATTGTGG GTCTCCAGTA CAAGAAAAAC
    TATGAAGATG AAGATTCATT GAAAACTCTT CGTTATGGGA AGATCATGAT TATGACAGAT
    CAGGACCAAG ATGGTTCCCA CATCAAAGGC TTGCTGATCA ATTTTATCCA TCACAATTGG
    CCATCTCTTC TGCGACATCG TTTTCTAGAA GAATTTATCA CTCCCATTGT AAAGGTGTCC
    AAAAATAAGC AAGAAATAGC ATTCTATAGT CTTCCCGAGT TTGAAGAATG GAAAAGTTCG
    ACTCCGAATC ATAAAAAGTG GAAAGTCAAA TACTACAAAG GTTTGGGCAC CAGCACATCA
    AAGGAAGCTA AGGAATATTT TGCAGATATG AAACGACATC GTATTCAGTT CAAATACTCT
    GGCCCTGAAG ATGATGCTGC GATCAGCCTG GCCTTTAGTA AAAAACAAGT TGATGATCGA
    AAGGAATGGT TAACTAATTT TATGGAAGAC AGAAGACAGC GGAAGTTACT TGGCCTTCCT
    GAGGATTATT TGTATGGACA AAGCACTTCG TACCTGACCT ATAATGACTT CATAAACAAG
    GAACTCATCC TCTTCTCCAA CTCTGATAAT GAAAGGTCTA TCCCATCCAT GGTAGACGGT
    TTGAAACCAG GTCAGAGAAA GGTTTTGTTT ACTTGTTTCA AACGGAATGA CAAGCGAGAA
    GTGAAGGTTG CCCAGTTAGC TGGGTCAGTG GCAGAAATGT CCTCTTATCA CCATGGTGAG
    ATGTCACTGA TGATGACCAT TATCAATTTG GCGCAGAATT TTGTGGGTAG CAATAATTTG
    AACCTTTTAC AGCCCATTGG TCAGTTTGGA ACCAGGCTGC ATGGTGGCAA GGACTCAGCT
    AGTCCTAGGT ACATCTTTAC AATGCTCAGC CCTTTGGCTC GGTTGTTGTT TCCTCCAAAA
    GATGATCACA CATTACGGTT TCTATATGAT GACAACCAAC GTGTTGAGCC TGAATGGTAC
    ATTCCTATAA TACCCATGGT GCTGATAAAT GGTGCTGAGG GAATTGGTAC TGGGTGGTCC
    TGCAAAATCC CCAACTTTGA TGTGCGTGAA GTTGTGAATA ATATCAGACG GCTTCTGGAT
    GGAGAGGAGC CCCTACCCAT GCTCCCAAGT TATAAGAATT TCAAAGGTAC TATTGAAGAA
    CTGGCTTCAA ATCAGTATGT GATTAATGGA GAAGTAGCTA TTCTGGATTC TACAACCATT
    GAAATCTCAG AGCTTCCCAT CCGAACGTGG ACTCAGACAT ATAAAGAACA GGTTCTAGAA
    CCCATGTTGA ATGGAACTGA GAAGACACCC TCTCTAATAA CAGACTATAG GGAATACCAT
    ACAGATACCA CTGTGAAGTT TGTCATAAAG ATGACTGAAG AAAAACTGGC AGAGGCAGAG
    AGAGTCGGCC TACACAAAGT CTTCAAACTC CAGAGTAGTC TCACTTGCAA CTCTATGGTG
    CTTTTTGACC ATGTAGGTTG CTTAAAGAAA TATGACACTG TGTTGGATAT TCTAAGAGAC
    TTCTTTGAGC TCAGGCTTAA ATATTATGGA TTAAGAAAAG AATGGCTTCT AGGAATGCTT
    GGTGCAGAAT CTTCTAAACT GAATAATCAA GCTCGCTTTA TATTAGAGAA AATAGATGGC
    AAAATAGTCA TTGAAAATAA ACCTAAAAAA GAATTAATTA AAGTTCTGAT TCAGAGAGGC
    TACGACTCTG ACCCTGTGAA AGCCTGGAAA GAAGCTCAGC AGAAGGTTCC AGATGAAGAA
    GAAAATGAAG AAAGTGACAC TGAAACCAGC ACCAGTGACT CTGCAGCCGA GGCTGGGCCG
    ACCTTCAACT ACCTTCTCGA CATGCCCCTG TGGTATCTGA CCAAGGAGAA GAAGGATGAG
    CTGTGCAAAC AAAGAAACGA GAAGGAACAA GAGCTCAACA CATTAAAGCA AAAGAGTCCA
    TCAGATCTGT GGAAGGAAGA TCTGGCTGTT TTTATTGAAG AACTGGAGGT TGTCGAGGCC
    AAGGAAAAGC AGGATGAACA AGTGGGCCTT CCTGGAAAGG CGGGGAAAGC AAAGGGGAAG
    AAAGCACAGA TGTGTGCTGA CGTCTTGCCT TCTCCTCGGG GCAAAAGAGT CATCCCCCAA
    GTGACTGTGG AGATGAAAGC TGAGGCAGAG AAGAAAATTA GGAAGAAAAT TAAGAGTGAA
    AACGTTGAGG GTACCCCTGC TGAGGATGGT GCGGAACCAG GAAGCCTCCG GCAACGAATA
    GAGAAGAAGC AGAAGAAAGA GCCAGGTGCA AAGAAGCAGA CTACATTGCC GTTTAAGCCT
    GTCAAAAAAG GGAGGAAGAA AAACCCCTGG TCTGACTCTG AGTCAGACGT GAGCAGTAAT
    GAAAGTAACG TTGATGTCCC TCCTCGACAA AAAGAGCAAA GAAGTGCTGC AGCAAAAGCC
    AAATTCACAG TGGATTTAGA CTCGGATGAA GATTTCTCAG GTTTGGATGA GAAGGATGAG
    GATGAAGATT TCCTCCCATT AGATGCTACT CCACCTAAGG CCAAAATCCC CCCAAAAAAT
    ACTAAAAAAG CACTGAAGAC CCAGGGAAGC TCCATGTCGG TTGATCTTGA AAGTGATGTT
    AAGGACAGTG TGCCAGCTTC TCCAGGCGTT CCTGCTGCTG ACTTCCCAGC GGAAACTGAA
    CAGTCAAAGC CATCCAAAAA GACCGTGGGA GTGAAGAAGA CAGCAACCAA AAGCCAGTCT
    TCAGTCTCCA CTGCTGGTAC CAAAAAGAGA GCTGCGCCAA AGGGAACCAA ATCAGATTCA
    GCCTTGAGTG CTCGTGTCTC GGAAAAACCT GCTCCTGCCA AAGCCAAGAA CAGTCGCAAA
    AGGAAGCCAT CTTCTTCTGA TAGCTCTGAC TCTGATTTTG AGAGAGCAAT TTCTAAAGGT
    GCCACGAGCA AGAAAGCAAA GGGAGAGGAA CAGGACTTCC CTGTGGACTT GGAAGACACG
    ATAGCTCCTC GAGCCAAATC TGACCGGGCG AGGAAGCCAA TTAAGTACCT GGAGGAGTCC
    GATGACGATG ACGACCTCTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.