Top1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Top1
Entrez Gene ID	21969
Full Name	topoisomerase (DNA) I
Synonyms	AI467334,D130064I21Rik,Top-1
General protein information	Preferred Names topoisomerase (DNA) I Names DNA topoisomerase 1 DNA topoisomerase I
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 H2\|2 80.96 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009408.2	NP_033434.2	DNA topoisomerase 1

Pathways from BioSystems

Homologies

	TOP1	XP_001088021.1
Bos taurus (cattle)	TOP1	NP_001193416.1
Gallus gallus (chicken)	TOP1	NP_990441.1
Neurospora crassa	NCU09118	XP_958033.2
Arabidopsis thaliana (thale cress)	TOP1ALPHA	NP_200341.1
Canis lupus familiaris (dog)	TOP1	XP_534420.3
Caenorhabditis elegans (roundworm)	top-1	NP_001021578.1
Saccharomyces cerevisiae (baker's yeast)	TOP1	NP_014637.1
Magnaporthe oryzae (rice blast fungus)	MGG_09917	XP_003710015.1
Arabidopsis thaliana (thale cress)	TOP1BETA	NP_200342.1
Kluyveromyces lactis	KLLA0C06116g	XP_452470.1
Eremothecium gossypii	AGOS_ACL008C	NP_983396.2
Xenopus tropicalis (tropical clawed frog)	LOC100496521	XP_002932918.2
Mus musculus (house mouse)	Top1	NP_033434.2
Danio rerio (zebrafish)	top1l	NP_001037789.1
Oryza sativa (rice)	Os08g0154600	NP_001061015.1
Homo sapiens (human)	TOP1	NP_003277.1
Pan troglodytes (chimpanzee)	TOP1	XP_514649.2
Rattus norvegicus (Norway rat)	Top1	NP_072137.1
Drosophila melanogaster (fruit fly)	Top1	NP_001014742.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004078	XP_003436707.1
Schizosaccharomyces pombe (fission yeast)	top1	NP_596209.1

Gene Ontology
Bibliography

Related articles in PubMed

Spartan deficiency causes accumulation of Topoisomerase 1 cleavage complexes and tumorigenesis.
Maskey RS, Flatten KS, Sieben CJ, Peterson KL, Baker DJ, Nam HJ, Kim MS, Smyrk TC, Kojima Y, Machida Y, Santiago A, van Deursen JM, Kaufmann SH, Machida YJ
Nucleic acids research45(8)4564-4576(2017 May)

The transcriptional regulator Aire binds to and activates super-enhancers.
Bansal K, Yoshida H, Benoist C, Mathis D
Nature immunology18(3)263-273(2017 Mar)

Topoisomerase 1 inhibitor topotecan delays the disease progression in a mouse model of Huntington's disease.
Shekhar S, Vatsa N, Kumar V, Singh BK, Jamal I, Sharma A, Jana NR
Human molecular genetics26(2)420-429(2017 01)

Topoisomerase 1 Regulates Gene Expression in Neurons through Cleavage Complex-Dependent and -Independent Mechanisms.
Mabb AM, Simon JM, King IF, Lee HM, An LK, Philpot BD, Zylka MJ
PloS one11(5)e0156439(2016)

Lack of mitochondrial topoisomerase I (TOP1mt) impairs liver regeneration.
Khiati S, Baechler SA, Factor VM, Zhang H, Huang SY, Dalla Rosa I, Sourbier C, Neckers L, Thorgeirsson SS, Pommier Y
Proceedings of the National Academy of Sciences of the United States of America112(36)11282-7(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It has been shown that the livers of Sprtn deficient mice accumulate DNA-protein crosslinks containing Topoisomerase 1 covalently linked to DNA.
Title: Spartan deficiency causes accumulation of Topoisomerase 1 cleavage complexes and tumorigenesis.

this study identified topoisomerase 1 as a cardinal Aire partner that colocalized on super-enhancers and was required for the interaction of Aire with all of its other associates
Title: The transcriptional regulator Aire binds to and activates super-enhancers.

treatment of topotecan, a brain-penetrating topoisomerase 1 inhibitor, to HD transgenic mouse considerably improved its motor behavioural abnormalities. Finally, we show that topotecan treatment to HD mouse not only inhibits the expression of transgenic mutant huntingtin, but also at the same time induces the expression of Ube3a
Title: Topoisomerase 1 inhibitor topotecan delays the disease progression in a mouse model of Huntington's disease.

TOP1cc-dependent and -independent contributions to gene expression
Title: Topoisomerase 1 Regulates Gene Expression in Neurons through Cleavage Complex-Dependent and -Independent Mechanisms.

Data indicate that mitochondrial topoisomerase I (TOP1mt) knockout (KO) mice showed a marked reduction in regeneration and hepatocyte proliferation.
Title: Lack of mitochondrial topoisomerase I (TOP1mt) impairs liver regeneration.

The following Top1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20658	NM_009408.2 Latest version!	Mus musculus topoisomerase (DNA) I (Top1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Top1 ( NM_009408.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033434.2 Mus musculus topoisomerase (DNA) I (Top1), mRNA.

Price & Availability↑

CloneID	OMu20658
Clone ID Related Accession (Same CDS sequence)	NM_009408.2
Accession Version	NM_009408.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	DNA topoisomerase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF733054.1, AK164061.1, AK145538.1, CO425650.1, CF734074.1, CN717304.1, AL590414.4 and DV645012.1. On Aug 18, 2006 this sequence version replaced NM_009408.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D10061.1, L20632.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGGGG ACCATCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
TCTCACAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCATAAGGA GCACAAGAAG 
GATAAGGATA AGGACCGGGA AAAGTCTAAG CATAGCAACA GTGAACATAA AGATTCTGAA 
AAGAAACACA AAGAGAAAGA GAAAACCAAA CACAAAGATG GCAGCTCAGA AAAACATAAA 
GACAAACATA AAGACAGAGA CAAGGAAAGA CGAAAGGAGG AAAAGATTAG AGCTGCTGGG 
GATGCAAAAA TAAAGAAGGA AAAGGAAAAT GGCTTCTCTA GTCCGCCACG AATTAAAGAT 
GAACCTGAGG ATGATGGCTA TTTTGCTCCT CCAAAAGAGG ATATTAAGCC ATTGAAGAGG 
CTTCGAGATG AAGATGATGC TGATTATAAA CCTAAGAAAA TTAAGACAGA AGATATCAAG 
AAGGAGAAGA AAAGAAAATC AGAAGAGGAA GAGGATGGTA AACTTAAAAA GCCCAAGAAT 
AAAGATAAAG ATAAAAAAGT TGCTGAGCCA GATAATAAGA AAAAGAAGCC GAAAAAGGAA 
GAGGAACAGA AGTGGAAATG GTGGGAAGAA GAACGTTATC CAGAAGGCAT CAAATGGAAA 
TTCCTAGAGC ATAAAGGGCC TGTCTTCGCT CCACCATATG AGCCTCTGCC AGAGAGTGTC 
AAGTTTTACT ATGATGGTAA AGTTATGAAG CTGAGTCCTA AAGCAGAAGA AGTAGCTACA 
TTCTTTGCAA AAATGCTTGA CCACGAATAT ACTACTAAGG AAATATTCAG GAAAAATTTC 
TTTAAAGATT GGAGAAAGGA AATGACTAAT GACGAAAAAA ATACGATTAC CAACCTAAGC 
AAATGCGACT TTACACAGAT GAGCCAGTAT TTCAAAGCGC AGTCAGAAGC TCGGAAACAG 
ATGAGCAAGG AAGAAAAATT GAAAATCAAA GAAGAAAATG AAAAGTTATT GAAAGAATAC 
GGCTTTTGTG TAATGGATAA TCATAGAGAG CGGATTGCCA ACTTCAAGAT AGAGCCTCCG 
GGGCTTTTCC GAGGCCGAGG GAACCACCCC AAGATGGGTA TGCTGAAGAG AAGGATCATG 
CCTGAGGACA TCATCATCAA CTGTAGCAAA GACGCAAAGG TTCCTTCTCC CCCTCCTGGA 
CATAAATGGA AGGAAGTCCG ACATGATAAC AAGGTTACTT GGCTGGTCTC CTGGACAGAG 
AATATCCAAG GTTCTATCAA ATATATCATG CTGAATCCCA GTTCACGAAT CAAGGGTGAG 
AAAGACTGGC AGAAGTATGA GACTGCGCGG CGTCTGAAGA AGTGTGTGGA CAAGATCCGA 
AATCAGTATC GGGAAGACTG GAAGTCCAAA GAGATGAAAG TTCGTCAGAG AGCTGTAGCA 
CTGTACTTCA TTGATAAGCT TGCTCTGAGA GCAGGCAATG AGAAGGAAGA AGGAGAGACG 
GCAGACACTG TGGGCTGCTG TTCACTTAGA GTGGAACACA TCAATTTACA CCCAGAATTG 
GATGGTCAGG AGTATGTGGT GGAATTTGAC TTTCCTGGGA AGGACTCAAT CAGATACTAT 
AACAAAGTCC CAGTTGAGAA ACGAGTTTTT AAAAACTTAC AACTGTTTAT GGAGAACAAG 
CAGCCTGAGG ACGATCTTTT TGATCGACTT AATACTGGTA TTCTAAATAA ACATCTTCAG 
GATCTCATGG AGGGCTTGAC TGCCAAGGTG TTCCGTACCT ACAATGCCTC CATCACACTA 
CAGCAGCAGC TTAAAGAGCT CACAGCCCCT GATGAGAATG TACCAGCGAA GATTCTATCT 
TATAACCGTG CCAATCGAGC TGTTGCAATT CTTTGTAACC ACCAGAGGGC GCCACCAAAG 
ACCTTTGAGA AGTCAATGAT GAACTTGCAG TCTAAGATTG ATGCCAAGAA AGATCAGTTA 
GCAGATGCTC GAAGGGACCT GAAAAGTGCT AAGGCTGATG CCAAAGTCAT GAAGGATGCA 
AAGACCAAGA AGGTAGTAGA GTCAAAAAAG AAGGCTGTAC AGAGACTAGA AGAGCAGTTG 
ATGAAGCTGG AAGTTCAAGC CACAGATCGA GAGGAGAACA AACAAATTGC CTTGGGGACC 
TCCAAACTCA ATTATCTGGA CCCTAGGATC ACAGTGGCTT GGTGCAAAAA GTGGGGGGTC 
CCAATTGAGA AGATTTACAA CAAAACCCAG AGGGAGAAGT TTGCTTGGGC CATTGATATG 
ACTGATGAGG ACTACGAGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033434.2
CDS	334..2637
Translation	MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKDKDKDREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKERRKEEKIRAAGDAKIKKEKENGFSSPPRIKDEPEDDGYFAPPKEDIKPLKRLRDEDDADYKPKKIKTEDIKKEKKRKSEEEEDGKLKKPKNKDKDKKVAEPDNKKKKPKKEEEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPESVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNDEKNTITNLSKCDFTQMSQYFKAQSEARKQMSKEEKLKIKEENEKLLKEYGFCVMDNHRERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKDQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMTDEDYEF

Target ORF information:

RefSeq Version	NM_009408.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus topoisomerase (DNA) I (Top1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009408.2

ORF Insert Sequence:

ATGAGCGGGG ACCATCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
TCTCACAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCATAAGGA GCACAAGAAG 
GATAAGGATA AGGACCGGGA AAAGTCTAAG CATAGCAACA GTGAACATAA AGATTCTGAA 
AAGAAACACA AAGAGAAAGA GAAAACCAAA CACAAAGATG GCAGCTCAGA AAAACATAAA 
GACAAACATA AAGACAGAGA CAAGGAAAGA CGAAAGGAGG AAAAGATTAG AGCTGCTGGG 
GATGCAAAAA TAAAGAAGGA AAAGGAAAAT GGCTTCTCTA GTCCGCCACG AATTAAAGAT 
GAACCTGAGG ATGATGGCTA TTTTGCTCCT CCAAAAGAGG ATATTAAGCC ATTGAAGAGG 
CTTCGAGATG AAGATGATGC TGATTATAAA CCTAAGAAAA TTAAGACAGA AGATATCAAG 
AAGGAGAAGA AAAGAAAATC AGAAGAGGAA GAGGATGGTA AACTTAAAAA GCCCAAGAAT 
AAAGATAAAG ATAAAAAAGT TGCTGAGCCA GATAATAAGA AAAAGAAGCC GAAAAAGGAA 
GAGGAACAGA AGTGGAAATG GTGGGAAGAA GAACGTTATC CAGAAGGCAT CAAATGGAAA 
TTCCTAGAGC ATAAAGGGCC TGTCTTCGCT CCACCATATG AGCCTCTGCC AGAGAGTGTC 
AAGTTTTACT ATGATGGTAA AGTTATGAAG CTGAGTCCTA AAGCAGAAGA AGTAGCTACA 
TTCTTTGCAA AAATGCTTGA CCACGAATAT ACTACTAAGG AAATATTCAG GAAAAATTTC 
TTTAAAGATT GGAGAAAGGA AATGACTAAT GACGAAAAAA ATACGATTAC CAACCTAAGC 
AAATGCGACT TTACACAGAT GAGCCAGTAT TTCAAAGCGC AGTCAGAAGC TCGGAAACAG 
ATGAGCAAGG AAGAAAAATT GAAAATCAAA GAAGAAAATG AAAAGTTATT GAAAGAATAC 
GGCTTTTGTG TAATGGATAA TCATAGAGAG CGGATTGCCA ACTTCAAGAT AGAGCCTCCG 
GGGCTTTTCC GAGGCCGAGG GAACCACCCC AAGATGGGTA TGCTGAAGAG AAGGATCATG 
CCTGAGGACA TCATCATCAA CTGTAGCAAA GACGCAAAGG TTCCTTCTCC CCCTCCTGGA 
CATAAATGGA AGGAAGTCCG ACATGATAAC AAGGTTACTT GGCTGGTCTC CTGGACAGAG 
AATATCCAAG GTTCTATCAA ATATATCATG CTGAATCCCA GTTCACGAAT CAAGGGTGAG 
AAAGACTGGC AGAAGTATGA GACTGCGCGG CGTCTGAAGA AGTGTGTGGA CAAGATCCGA 
AATCAGTATC GGGAAGACTG GAAGTCCAAA GAGATGAAAG TTCGTCAGAG AGCTGTAGCA 
CTGTACTTCA TTGATAAGCT TGCTCTGAGA GCAGGCAATG AGAAGGAAGA AGGAGAGACG 
GCAGACACTG TGGGCTGCTG TTCACTTAGA GTGGAACACA TCAATTTACA CCCAGAATTG 
GATGGTCAGG AGTATGTGGT GGAATTTGAC TTTCCTGGGA AGGACTCAAT CAGATACTAT 
AACAAAGTCC CAGTTGAGAA ACGAGTTTTT AAAAACTTAC AACTGTTTAT GGAGAACAAG 
CAGCCTGAGG ACGATCTTTT TGATCGACTT AATACTGGTA TTCTAAATAA ACATCTTCAG 
GATCTCATGG AGGGCTTGAC TGCCAAGGTG TTCCGTACCT ACAATGCCTC CATCACACTA 
CAGCAGCAGC TTAAAGAGCT CACAGCCCCT GATGAGAATG TACCAGCGAA GATTCTATCT 
TATAACCGTG CCAATCGAGC TGTTGCAATT CTTTGTAACC ACCAGAGGGC GCCACCAAAG 
ACCTTTGAGA AGTCAATGAT GAACTTGCAG TCTAAGATTG ATGCCAAGAA AGATCAGTTA 
GCAGATGCTC GAAGGGACCT GAAAAGTGCT AAGGCTGATG CCAAAGTCAT GAAGGATGCA 
AAGACCAAGA AGGTAGTAGA GTCAAAAAAG AAGGCTGTAC AGAGACTAGA AGAGCAGTTG 
ATGAAGCTGG AAGTTCAAGC CACAGATCGA GAGGAGAACA AACAAATTGC CTTGGGGACC 
TCCAAACTCA ATTATCTGGA CCCTAGGATC ACAGTGGCTT GGTGCAAAAA GTGGGGGGTC 
CCAATTGAGA AGATTTACAA CAAAACCCAG AGGGAGAAGT TTGCTTGGGC CATTGATATG 
ACTGATGAGG ACTACGAGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spartan deficiency causes accumulation of Topoisomerase 1 cleavage complexes and tumorigenesis.
Nucleic acids research45(8)4564-4576(2017 May)
Maskey RS,Flatten KS,Sieben CJ,Peterson KL,Baker DJ,Nam HJ,Kim MS,Smyrk TC,Kojima Y,Machida Y,Santiago A,van Deursen JM,Kaufmann SH,Machida YJ

The transcriptional regulator Aire binds to and activates super-enhancers.
Nature immunology18(3)263-273(2017 Mar)
Bansal K,Yoshida H,Benoist C,Mathis D

Topoisomerase 1 inhibitor topotecan delays the disease progression in a mouse model of Huntington's disease.
Human molecular genetics26(2)420-429(2017 01)
Shekhar S,Vatsa N,Kumar V,Singh BK,Jamal I,Sharma A,Jana NR

Topoisomerase 1 Regulates Gene Expression in Neurons through Cleavage Complex-Dependent and -Independent Mechanisms.
PloS one11(5)e0156439(2016)
Mabb AM,Simon JM,King IF,Lee HM,An LK,Philpot BD,Zylka MJ

Lack of mitochondrial topoisomerase I (TOP1mt) impairs liver regeneration.
Proceedings of the National Academy of Sciences of the United States of America112(36)11282-7(2015 Sep)
Khiati S,Baechler SA,Factor VM,Zhang H,Huang SY,Dalla Rosa I,Sourbier C,Neckers L,Thorgeirsson SS,Pommier Y

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Top1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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