Top1 cDNA ORF clone, Mus musculus(house mouse)

The following Top1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu20658 NM_009408.2
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Mus musculus topoisomerase (DNA) I (Top1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu20658
    Clone ID Related Accession (Same CDS sequence) NM_009408.2
    Accession Version NM_009408.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Mus musculus(house mouse)
    Product DNA topoisomerase 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF733054.1, AK164061.1, AK145538.1, CO425650.1, CF734074.1, CN717304.1, AL590414.4 and DV645012.1. On Aug 18, 2006 this sequence version replaced NM_009408.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D10061.1, L20632.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCGGGG ACCATCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
    TCTCACAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCATAAGGA GCACAAGAAG
    GATAAGGATA AGGACCGGGA AAAGTCTAAG CATAGCAACA GTGAACATAA AGATTCTGAA
    AAGAAACACA AAGAGAAAGA GAAAACCAAA CACAAAGATG GCAGCTCAGA AAAACATAAA
    GACAAACATA AAGACAGAGA CAAGGAAAGA CGAAAGGAGG AAAAGATTAG AGCTGCTGGG
    GATGCAAAAA TAAAGAAGGA AAAGGAAAAT GGCTTCTCTA GTCCGCCACG AATTAAAGAT
    GAACCTGAGG ATGATGGCTA TTTTGCTCCT CCAAAAGAGG ATATTAAGCC ATTGAAGAGG
    CTTCGAGATG AAGATGATGC TGATTATAAA CCTAAGAAAA TTAAGACAGA AGATATCAAG
    AAGGAGAAGA AAAGAAAATC AGAAGAGGAA GAGGATGGTA AACTTAAAAA GCCCAAGAAT
    AAAGATAAAG ATAAAAAAGT TGCTGAGCCA GATAATAAGA AAAAGAAGCC GAAAAAGGAA
    GAGGAACAGA AGTGGAAATG GTGGGAAGAA GAACGTTATC CAGAAGGCAT CAAATGGAAA
    TTCCTAGAGC ATAAAGGGCC TGTCTTCGCT CCACCATATG AGCCTCTGCC AGAGAGTGTC
    AAGTTTTACT ATGATGGTAA AGTTATGAAG CTGAGTCCTA AAGCAGAAGA AGTAGCTACA
    TTCTTTGCAA AAATGCTTGA CCACGAATAT ACTACTAAGG AAATATTCAG GAAAAATTTC
    TTTAAAGATT GGAGAAAGGA AATGACTAAT GACGAAAAAA ATACGATTAC CAACCTAAGC
    AAATGCGACT TTACACAGAT GAGCCAGTAT TTCAAAGCGC AGTCAGAAGC TCGGAAACAG
    ATGAGCAAGG AAGAAAAATT GAAAATCAAA GAAGAAAATG AAAAGTTATT GAAAGAATAC
    GGCTTTTGTG TAATGGATAA TCATAGAGAG CGGATTGCCA ACTTCAAGAT AGAGCCTCCG
    GGGCTTTTCC GAGGCCGAGG GAACCACCCC AAGATGGGTA TGCTGAAGAG AAGGATCATG
    CCTGAGGACA TCATCATCAA CTGTAGCAAA GACGCAAAGG TTCCTTCTCC CCCTCCTGGA
    CATAAATGGA AGGAAGTCCG ACATGATAAC AAGGTTACTT GGCTGGTCTC CTGGACAGAG
    AATATCCAAG GTTCTATCAA ATATATCATG CTGAATCCCA GTTCACGAAT CAAGGGTGAG
    AAAGACTGGC AGAAGTATGA GACTGCGCGG CGTCTGAAGA AGTGTGTGGA CAAGATCCGA
    AATCAGTATC GGGAAGACTG GAAGTCCAAA GAGATGAAAG TTCGTCAGAG AGCTGTAGCA
    CTGTACTTCA TTGATAAGCT TGCTCTGAGA GCAGGCAATG AGAAGGAAGA AGGAGAGACG
    GCAGACACTG TGGGCTGCTG TTCACTTAGA GTGGAACACA TCAATTTACA CCCAGAATTG
    GATGGTCAGG AGTATGTGGT GGAATTTGAC TTTCCTGGGA AGGACTCAAT CAGATACTAT
    AACAAAGTCC CAGTTGAGAA ACGAGTTTTT AAAAACTTAC AACTGTTTAT GGAGAACAAG
    CAGCCTGAGG ACGATCTTTT TGATCGACTT AATACTGGTA TTCTAAATAA ACATCTTCAG
    GATCTCATGG AGGGCTTGAC TGCCAAGGTG TTCCGTACCT ACAATGCCTC CATCACACTA
    CAGCAGCAGC TTAAAGAGCT CACAGCCCCT GATGAGAATG TACCAGCGAA GATTCTATCT
    TATAACCGTG CCAATCGAGC TGTTGCAATT CTTTGTAACC ACCAGAGGGC GCCACCAAAG
    ACCTTTGAGA AGTCAATGAT GAACTTGCAG TCTAAGATTG ATGCCAAGAA AGATCAGTTA
    GCAGATGCTC GAAGGGACCT GAAAAGTGCT AAGGCTGATG CCAAAGTCAT GAAGGATGCA
    AAGACCAAGA AGGTAGTAGA GTCAAAAAAG AAGGCTGTAC AGAGACTAGA AGAGCAGTTG
    ATGAAGCTGG AAGTTCAAGC CACAGATCGA GAGGAGAACA AACAAATTGC CTTGGGGACC
    TCCAAACTCA ATTATCTGGA CCCTAGGATC ACAGTGGCTT GGTGCAAAAA GTGGGGGGTC
    CCAATTGAGA AGATTTACAA CAAAACCCAG AGGGAGAAGT TTGCTTGGGC CATTGATATG
    ACTGATGAGG ACTACGAGTT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_033434.2
    CDS334..2637
    Translation

    Target ORF information:

    RefSeq Version NM_009408.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus topoisomerase (DNA) I (Top1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_009408.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCGGGG ACCATCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
    TCTCACAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCATAAGGA GCACAAGAAG
    GATAAGGATA AGGACCGGGA AAAGTCTAAG CATAGCAACA GTGAACATAA AGATTCTGAA
    AAGAAACACA AAGAGAAAGA GAAAACCAAA CACAAAGATG GCAGCTCAGA AAAACATAAA
    GACAAACATA AAGACAGAGA CAAGGAAAGA CGAAAGGAGG AAAAGATTAG AGCTGCTGGG
    GATGCAAAAA TAAAGAAGGA AAAGGAAAAT GGCTTCTCTA GTCCGCCACG AATTAAAGAT
    GAACCTGAGG ATGATGGCTA TTTTGCTCCT CCAAAAGAGG ATATTAAGCC ATTGAAGAGG
    CTTCGAGATG AAGATGATGC TGATTATAAA CCTAAGAAAA TTAAGACAGA AGATATCAAG
    AAGGAGAAGA AAAGAAAATC AGAAGAGGAA GAGGATGGTA AACTTAAAAA GCCCAAGAAT
    AAAGATAAAG ATAAAAAAGT TGCTGAGCCA GATAATAAGA AAAAGAAGCC GAAAAAGGAA
    GAGGAACAGA AGTGGAAATG GTGGGAAGAA GAACGTTATC CAGAAGGCAT CAAATGGAAA
    TTCCTAGAGC ATAAAGGGCC TGTCTTCGCT CCACCATATG AGCCTCTGCC AGAGAGTGTC
    AAGTTTTACT ATGATGGTAA AGTTATGAAG CTGAGTCCTA AAGCAGAAGA AGTAGCTACA
    TTCTTTGCAA AAATGCTTGA CCACGAATAT ACTACTAAGG AAATATTCAG GAAAAATTTC
    TTTAAAGATT GGAGAAAGGA AATGACTAAT GACGAAAAAA ATACGATTAC CAACCTAAGC
    AAATGCGACT TTACACAGAT GAGCCAGTAT TTCAAAGCGC AGTCAGAAGC TCGGAAACAG
    ATGAGCAAGG AAGAAAAATT GAAAATCAAA GAAGAAAATG AAAAGTTATT GAAAGAATAC
    GGCTTTTGTG TAATGGATAA TCATAGAGAG CGGATTGCCA ACTTCAAGAT AGAGCCTCCG
    GGGCTTTTCC GAGGCCGAGG GAACCACCCC AAGATGGGTA TGCTGAAGAG AAGGATCATG
    CCTGAGGACA TCATCATCAA CTGTAGCAAA GACGCAAAGG TTCCTTCTCC CCCTCCTGGA
    CATAAATGGA AGGAAGTCCG ACATGATAAC AAGGTTACTT GGCTGGTCTC CTGGACAGAG
    AATATCCAAG GTTCTATCAA ATATATCATG CTGAATCCCA GTTCACGAAT CAAGGGTGAG
    AAAGACTGGC AGAAGTATGA GACTGCGCGG CGTCTGAAGA AGTGTGTGGA CAAGATCCGA
    AATCAGTATC GGGAAGACTG GAAGTCCAAA GAGATGAAAG TTCGTCAGAG AGCTGTAGCA
    CTGTACTTCA TTGATAAGCT TGCTCTGAGA GCAGGCAATG AGAAGGAAGA AGGAGAGACG
    GCAGACACTG TGGGCTGCTG TTCACTTAGA GTGGAACACA TCAATTTACA CCCAGAATTG
    GATGGTCAGG AGTATGTGGT GGAATTTGAC TTTCCTGGGA AGGACTCAAT CAGATACTAT
    AACAAAGTCC CAGTTGAGAA ACGAGTTTTT AAAAACTTAC AACTGTTTAT GGAGAACAAG
    CAGCCTGAGG ACGATCTTTT TGATCGACTT AATACTGGTA TTCTAAATAA ACATCTTCAG
    GATCTCATGG AGGGCTTGAC TGCCAAGGTG TTCCGTACCT ACAATGCCTC CATCACACTA
    CAGCAGCAGC TTAAAGAGCT CACAGCCCCT GATGAGAATG TACCAGCGAA GATTCTATCT
    TATAACCGTG CCAATCGAGC TGTTGCAATT CTTTGTAACC ACCAGAGGGC GCCACCAAAG
    ACCTTTGAGA AGTCAATGAT GAACTTGCAG TCTAAGATTG ATGCCAAGAA AGATCAGTTA
    GCAGATGCTC GAAGGGACCT GAAAAGTGCT AAGGCTGATG CCAAAGTCAT GAAGGATGCA
    AAGACCAAGA AGGTAGTAGA GTCAAAAAAG AAGGCTGTAC AGAGACTAGA AGAGCAGTTG
    ATGAAGCTGG AAGTTCAAGC CACAGATCGA GAGGAGAACA AACAAATTGC CTTGGGGACC
    TCCAAACTCA ATTATCTGGA CCCTAGGATC ACAGTGGCTT GGTGCAAAAA GTGGGGGGTC
    CCAATTGAGA AGATTTACAA CAAAACCCAG AGGGAGAAGT TTGCTTGGGC CATTGATATG
    ACTGATGAGG ACTACGAGTT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.