Pcdh20 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcdh20
Entrez Gene ID	219257
Full Name	protocadherin 20
Synonyms	C630015B17Rik,Pcdh13
General protein information	Preferred Names protocadherin 20 Names protocadherin-20 protocadherin 13
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 E1
Summary	This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The encoded protein contains six extracellular cadherin domains, a transmembrane domain, and a cytoplasmic tail differing from those of the classical cadherins. The encoded protein may play a role in cell adhesion in the nervous system and has been shown to be specifically expressed in newly differentiated olfactory sensory neurons and their axons during development. In adult mice, the expression of this protein in the olfactory system is more restricted but shows a gender difference with higher expression in the male than in the female. [provided by RefSeq, Sep 2009].

mRNA and Protein(s)

mRNA	Protein	Name
NM_178685.5	NP_848800.3	protocadherin-20 precursor
XM_030247774.1	XP_030103634.1	protocadherin-20 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PCDH20	NP_073754.2
Canis lupus familiaris (dog)	LOC100686939	XP_005634010.1
Xenopus tropicalis (tropical clawed frog)	pcdh20	NP_001093700.1
Rattus norvegicus (Norway rat)	Pcdh20	NP_001100750.1
Gallus gallus (chicken)	PCDH20	XP_004938713.1
Pan troglodytes (chimpanzee)	PCDH20	XP_003314208.1
	PCDH20	XP_001086611.1
Bos taurus (cattle)	PCDH20	NP_001193160.1
Mus musculus (house mouse)	Pcdh20	NP_848800.3
Danio rerio (zebrafish)	pcdh20	XP_001345843.3

Gene Ontology
Bibliography

Related articles in PubMed

Molecular and Neural Functions of Rai1, the Causal Gene for Smith-Magenis Syndrome.
Huang WH, Guenthner CJ, Xu J, Nguyen T, Schwarz LA, Wilkinson AW, Gozani O, Chang HY, Shamloo M, Luo L
Neuron92(2)392-406(2016 Oct)

Context-specific function of the LIM homeobox 1 transcription factor in head formation of the mouse embryo.
Fossat N, Ip CK, Jones VJ, Studdert JB, Khoo PL, Lewis SL, Power M, Tourle K, Loebel DA, Kwan KM, Behringer RR, Tam PP
Development (Cambridge, England)142(11)2069-79(2015 Jun)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Secreted TARSH regulates olfactory mitral cell dendritic complexity.
Cheng TW, Gong Q
The European journal of neuroscience29(6)1083-95(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The gender difference in PCDH20 expression may reflect olfactory receptor expression differences for gender-specific social discrimination
Title: Olfactory sensory neuron-specific and sexually dimorphic expression of protocadherin 20.

The following Pcdh20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcdh20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07559	NM_178685.5 Latest version!	Mus musculus protocadherin 20 (Pcdh20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu86411	XM_030247774.1 Latest version!	Mus musculus protocadherin 20 (Pcdh20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcdh20 ( NM_178685.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_848800.3 Mus musculus protocadherin 20 (Pcdh20), mRNA.

Price & Availability↑

CloneID	OMu07559
Clone ID Related Accession (Same CDS sequence)	NM_178685.5
Accession Version	NM_178685.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Mus musculus(house mouse)
Product	protocadherin-20 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK083114.1, BC079605.1 and AC164553.2. On Oct 8, 2009 this sequence version replaced NM_178685.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK083114.1, BB653201.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164132, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCGGCC GAGGGAACGC GCGCAGCTTG CTGGTCCAGG CAGTGAGCTT GCGCCCGGCG 
ACCTGGCACC CGTGCCTGGA TATGGGGCAT CTACATCGTC CCAGCAGCAG GACCAGCCAC 
AGGAACCTGC CGCATGTTTT TCTGCTTTTC CTCTTCGTGG GACCCTTCAA TTGCCTCGCG 
AGTTACAGCC GTGCTACCGA GCTTCTGTAC AGCCTAAATG AGGGACTGCC CGCCGGAGTG 
CTCATAGGCA GCCTGGCGGA GGACCTGAGG CTGCTGCCTC GGGCCTCTGG GAGACAGAAC 
CAGCAGTTGC TGCATCCAGA GCGCACTGCC TCTGAGGGGA ACCCCCCTCT CTCTTTCAGC 
TTGGCCTCCG GGGGACTGAG TGGCCAGTAT GTGACCCTAA ACAACCGCTC CGGCGAGCTG 
CACACTTCTG CACAGGAGAT CGACAGAGAA GCATTGTGCC TGGATGGGGG CGGTGGGGCT 
GCCTGGGCCG GCAGTACCTC CATTGCCTCC TCTCCTTCTT CTGACTCTTG CCTTTTGCTT 
CTGGACGTGC TGGTCCTGCC TCAGGAATAT TTTCGGTTTG TGAAGGTGAA AATTGCCATC 
AGGGATATCA ATGACAATGC TCCGCAGTTC CCCATTTCTG AAATCTCTGT TTGGGTCCCA 
GAAAATTCAC CTGTAAATAC CCGATTGGCT ATAGAGCATC CTGCTGTGGA CCCTGATGTA 
GGCATTAACG GTGTGCAAAC CTATCGCTTA CTAGACTACC ATGGCATGTT TACCCTGGAC 
GTGGAGGAGA ATGAGAACGG GGAGCGCACA CCCTACTTGA TTGTCATGGG TGCTTTGGAC 
AGAGAAACCC AGGACCAGTA TGTGAGCATC ATCATAGCTG AGGATGGTGG GTCTCCACCA 
CTGCTGGGCA GTGCCACCCT CACCATTGGC ATAAGTGACA TTAATGACAA TTGTCCTCTC 
TTTATAGACT CACAAATTAA TGTCACCGTT TATGGGAATG CTACAGTGGG CACCCCAGTT 
GCAACAGTCC AGGCTGTGGA TAGAGACTTG GGGACAAATG CACAGATCAC TTACAGTTAC 
AGCCAGAAAG TTCCACAGGA AACCAAGGAT TTATTCCACC TGGATGAAGT TACTGGAGTC 
ATTAAGCTTT CCAGTAAGAT TGGCGGGAGT GTCCTACAGA CACATAAGCT CACCATCCTA 
GCTAATGGAC CTGGCTGTAT CCCTGCAGTG ATCACTGCTC TTGTATCGAT CATCAAAGTC 
GTTTTCAGAC CCCCAGAAAT CGTCCCACGT TATATCGCAA ATGAGGTGGA TGGTGTTGTG 
TACCTGAAAG AACTGGAGCC TGTTAATACT CCAATTGCGT TTTTTACCAT CAGAGATCCA 
GAAGGTAAAT ACAAGATTAA CTGCTTCTTG GATGGTGAAG GGCCATTCAG GCTGGCACCC 
TACAAGCCGT ACAACAATGA ATATTTGTTG GAGACCACAA AGCCTATGGA CTATGAAGTG 
CAGTCCTTCT ATGAAATAGC TGTAGTGGCC TGGAACTCAG AGGGATTTCA TGTCAAGAGA 
ATCATTAAAG TACAACTTTT AGACGACAAT GACAATGCCC CTGTTTTCCT TCAGCCCTTG 
ATAGAATTGA CCATTGAAGA AAACAACGCA CCCAATGCCT TTCTGACAAA GCTCTACGCT 
ACAGATGCTG ACAGTGGAGA GATGGGACGA GTTTCCTACT TCTTGGGACC TGATGCTCCG 
TCATACTTTT CCTTAGACAG TGTCACAGGG ATTCTGACAG TTTCTACTCA GCTGGACAGA 
GAAGAGAAAG AGAAGTACAG GTATACTGTC CGAGCAGTGG ACTGTGGGAC GCCACCCAGA 
GAATCAGTGG CTACAGTGGC CCTCACTGTG TTGGATAAAA ATGACAACAG TCCGAGGTTC 
ATCAACAAGG ACTTCAGCTT TTTTGTCCCA GAAAACTTTC CGGGGTATGG TGAAATTGGA 
GTCATCAGTG TAACAGATGC CGATGCTGGA AGGAACGGAT GGGTAGCCCT GTCCGTAATG 
AACCAGAGTG ACATTTTTGT CATAGACACA GGCAAGGGTA TGTTGAGAGC TAAAGTCTCT 
TTAGACAGAG AACAGCAAAG CTCTTACACT CTGTGGGTGG AGGCTGTTGA CGGGGGTGAG 
CCTGCCCTTT CCTCTACCAC AAAAATCACA ATTCTTCTTC TGGACATCAA CGATAACCCT 
CCTCTTGTTT TATTCCCTCA ATCTAATATG TCTTATTTGC TGGTCCTACC TTCTACTCTC 
CCTGGCTCAC CAGTAACAGA AGTCTATGCG GTTGACAAAG ACACAGGCAT GAATGCTGTC 
ATAGCTTATA GCATCATAGG GAGAAGAGGT CCCAGGCCTG AGTCTTTCAG AATTGACCCC 
AAGACTGGCA ACATCACTCT GGAGGAAGCC TTGCTTCAGA CAGATTATGG GTTGCATCGC 
TTACTGGTGA AAGTGAGTGA TCACGGATAC CCTGAGCCTC TCCACTCCAC AGTCATGGTG 
AACTTATTTG TCAATGACAC TGTCAGTAAC GAGAGCTACA TTGAGAGCCT TTTGAGAAAG 
GAGCCAGAAA TTAATATAGA AGAGAAAGAA CCACAAATCT CAATAGAGCC AACTCATAGG 
AAGGTAGAAT CTATGTCCTG TATGCCCACA TTGGTAGCTC TGTCAGTCAT AAGCTTGGGC 
TCCATCACAC TAGTTACAGG GATGGGAATA TACATCTGCT TACGGAAAGG TAAAAAGCAT 
CACAGGGAAG ATGACAATTT GGAAGTACAG ATTCCATTGA AAGGAAAAAT TGACTTGTGT 
ATGAGAGAGA GAAAACCAGT GGATATTTCT AACATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848800.3
CDS	122..2980
Translation	MRGRGNARSLLVQAVSLRPATWHPCLDMGHLHRPSSRTSHRNLPHVFLLFLFVGPFNCLASYSRATELLYSLNEGLPAGVLIGSLAEDLRLLPRASGRQNQQLLHPERTASEGNPPLSFSLASGGLSGQYVTLNNRSGELHTSAQEIDREALCLDGGGGAAWAGSTSIASSPSSDSCLLLLDVLVLPQEYFRFVKVKIAIRDINDNAPQFPISEISVWVPENSPVNTRLAIEHPAVDPDVGINGVQTYRLLDYHGMFTLDVEENENGERTPYLIVMGALDRETQDQYVSIIIAEDGGSPPLLGSATLTIGISDINDNCPLFIDSQINVTVYGNATVGTPVATVQAVDRDLGTNAQITYSYSQKVPQETKDLFHLDEVTGVIKLSSKIGGSVLQTHKLTILANGPGCIPAVITALVSIIKVVFRPPEIVPRYIANEVDGVVYLKELEPVNTPIAFFTIRDPEGKYKINCFLDGEGPFRLAPYKPYNNEYLLETTKPMDYEVQSFYEIAVVAWNSEGFHVKRIIKVQLLDDNDNAPVFLQPLIELTIEENNAPNAFLTKLYATDADSGEMGRVSYFLGPDAPSYFSLDSVTGILTVSTQLDREEKEKYRYTVRAVDCGTPPRESVATVALTVLDKNDNSPRFINKDFSFFVPENFPGYGEIGVISVTDADAGRNGWVALSVMNQSDIFVIDTGKGMLRAKVSLDREQQSSYTLWVEAVDGGEPALSSTTKITILLLDINDNPPLVLFPQSNMSYLLVLPSTLPGSPVTEVYAVDKDTGMNAVIAYSIIGRRGPRPESFRIDPKTGNITLEEALLQTDYGLHRLLVKVSDHGYPEPLHSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQISIEPTHRKVESMSCMPTLVALSVISLGSITLVTGMGIYICLRKGKKHHREDDNLEVQIPLKGKIDLCMRERKPVDISNI

Target ORF information:

RefSeq Version	NM_178685.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus protocadherin 20 (Pcdh20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178685.5

ORF Insert Sequence:

ATGCGCGGCC GAGGGAACGC GCGCAGCTTG CTGGTCCAGG CAGTGAGCTT GCGCCCGGCG 
ACCTGGCACC CGTGCCTGGA TATGGGGCAT CTACATCGTC CCAGCAGCAG GACCAGCCAC 
AGGAACCTGC CGCATGTTTT TCTGCTTTTC CTCTTCGTGG GACCCTTCAA TTGCCTCGCG 
AGTTACAGCC GTGCTACCGA GCTTCTGTAC AGCCTAAATG AGGGACTGCC CGCCGGAGTG 
CTCATAGGCA GCCTGGCGGA GGACCTGAGG CTGCTGCCTC GGGCCTCTGG GAGACAGAAC 
CAGCAGTTGC TGCATCCAGA GCGCACTGCC TCTGAGGGGA ACCCCCCTCT CTCTTTCAGC 
TTGGCCTCCG GGGGACTGAG TGGCCAGTAT GTGACCCTAA ACAACCGCTC CGGCGAGCTG 
CACACTTCTG CACAGGAGAT CGACAGAGAA GCATTGTGCC TGGATGGGGG CGGTGGGGCT 
GCCTGGGCCG GCAGTACCTC CATTGCCTCC TCTCCTTCTT CTGACTCTTG CCTTTTGCTT 
CTGGACGTGC TGGTCCTGCC TCAGGAATAT TTTCGGTTTG TGAAGGTGAA AATTGCCATC 
AGGGATATCA ATGACAATGC TCCGCAGTTC CCCATTTCTG AAATCTCTGT TTGGGTCCCA 
GAAAATTCAC CTGTAAATAC CCGATTGGCT ATAGAGCATC CTGCTGTGGA CCCTGATGTA 
GGCATTAACG GTGTGCAAAC CTATCGCTTA CTAGACTACC ATGGCATGTT TACCCTGGAC 
GTGGAGGAGA ATGAGAACGG GGAGCGCACA CCCTACTTGA TTGTCATGGG TGCTTTGGAC 
AGAGAAACCC AGGACCAGTA TGTGAGCATC ATCATAGCTG AGGATGGTGG GTCTCCACCA 
CTGCTGGGCA GTGCCACCCT CACCATTGGC ATAAGTGACA TTAATGACAA TTGTCCTCTC 
TTTATAGACT CACAAATTAA TGTCACCGTT TATGGGAATG CTACAGTGGG CACCCCAGTT 
GCAACAGTCC AGGCTGTGGA TAGAGACTTG GGGACAAATG CACAGATCAC TTACAGTTAC 
AGCCAGAAAG TTCCACAGGA AACCAAGGAT TTATTCCACC TGGATGAAGT TACTGGAGTC 
ATTAAGCTTT CCAGTAAGAT TGGCGGGAGT GTCCTACAGA CACATAAGCT CACCATCCTA 
GCTAATGGAC CTGGCTGTAT CCCTGCAGTG ATCACTGCTC TTGTATCGAT CATCAAAGTC 
GTTTTCAGAC CCCCAGAAAT CGTCCCACGT TATATCGCAA ATGAGGTGGA TGGTGTTGTG 
TACCTGAAAG AACTGGAGCC TGTTAATACT CCAATTGCGT TTTTTACCAT CAGAGATCCA 
GAAGGTAAAT ACAAGATTAA CTGCTTCTTG GATGGTGAAG GGCCATTCAG GCTGGCACCC 
TACAAGCCGT ACAACAATGA ATATTTGTTG GAGACCACAA AGCCTATGGA CTATGAAGTG 
CAGTCCTTCT ATGAAATAGC TGTAGTGGCC TGGAACTCAG AGGGATTTCA TGTCAAGAGA 
ATCATTAAAG TACAACTTTT AGACGACAAT GACAATGCCC CTGTTTTCCT TCAGCCCTTG 
ATAGAATTGA CCATTGAAGA AAACAACGCA CCCAATGCCT TTCTGACAAA GCTCTACGCT 
ACAGATGCTG ACAGTGGAGA GATGGGACGA GTTTCCTACT TCTTGGGACC TGATGCTCCG 
TCATACTTTT CCTTAGACAG TGTCACAGGG ATTCTGACAG TTTCTACTCA GCTGGACAGA 
GAAGAGAAAG AGAAGTACAG GTATACTGTC CGAGCAGTGG ACTGTGGGAC GCCACCCAGA 
GAATCAGTGG CTACAGTGGC CCTCACTGTG TTGGATAAAA ATGACAACAG TCCGAGGTTC 
ATCAACAAGG ACTTCAGCTT TTTTGTCCCA GAAAACTTTC CGGGGTATGG TGAAATTGGA 
GTCATCAGTG TAACAGATGC CGATGCTGGA AGGAACGGAT GGGTAGCCCT GTCCGTAATG 
AACCAGAGTG ACATTTTTGT CATAGACACA GGCAAGGGTA TGTTGAGAGC TAAAGTCTCT 
TTAGACAGAG AACAGCAAAG CTCTTACACT CTGTGGGTGG AGGCTGTTGA CGGGGGTGAG 
CCTGCCCTTT CCTCTACCAC AAAAATCACA ATTCTTCTTC TGGACATCAA CGATAACCCT 
CCTCTTGTTT TATTCCCTCA ATCTAATATG TCTTATTTGC TGGTCCTACC TTCTACTCTC 
CCTGGCTCAC CAGTAACAGA AGTCTATGCG GTTGACAAAG ACACAGGCAT GAATGCTGTC 
ATAGCTTATA GCATCATAGG GAGAAGAGGT CCCAGGCCTG AGTCTTTCAG AATTGACCCC 
AAGACTGGCA ACATCACTCT GGAGGAAGCC TTGCTTCAGA CAGATTATGG GTTGCATCGC 
TTACTGGTGA AAGTGAGTGA TCACGGATAC CCTGAGCCTC TCCACTCCAC AGTCATGGTG 
AACTTATTTG TCAATGACAC TGTCAGTAAC GAGAGCTACA TTGAGAGCCT TTTGAGAAAG 
GAGCCAGAAA TTAATATAGA AGAGAAAGAA CCACAAATCT CAATAGAGCC AACTCATAGG 
AAGGTAGAAT CTATGTCCTG TATGCCCACA TTGGTAGCTC TGTCAGTCAT AAGCTTGGGC 
TCCATCACAC TAGTTACAGG GATGGGAATA TACATCTGCT TACGGAAAGG TAAAAAGCAT 
CACAGGGAAG ATGACAATTT GGAAGTACAG ATTCCATTGA AAGGAAAAAT TGACTTGTGT 
ATGAGAGAGA GAAAACCAGT GGATATTTCT AACATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcdh20 ( XM_030247774.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103634.1 Mus musculus protocadherin 20 (Pcdh20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu86411
Clone ID Related Accession (Same CDS sequence)	XM_030247774.1
Accession Version	XM_030247774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protocadherin-20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCC TGGACGTGGA GGAGAATGAG AACGGGGAGC GCACACCCTA CTTGATTGTC 
ATGGGTGCTT TGGACAGAGA AACCCAGGAC CAGTATGTGA GCATCATCAT AGCTGAGGAT 
GGTGGGTCTC CACCACTGCT GGGCAGTGCC ACCCTCACCA TTGGCATAAG TGACATTAAT 
GACAATTGTC CTCTCTTTAT AGACTCACAA ATTAATGTCA CCGTTTATGG GAATGCTACA 
GTGGGCACCC CAGTTGCAAC AGTCCAGGCT GTGGATAGAG ACTTGGGGAC AAATGCACAG 
ATCACTTACA GTTACAGCCA GAAAGTTCCA CAGGAAACCA AGGATTTATT CCACCTGGAT 
GAAGTTACTG GAGTCATTAA GCTTTCCAGT AAGATTGGCG GGAGTGTCCT ACAGACACAT 
AAGCTCACCA TCCTAGCTAA TGGACCTGGC TGTATCCCTG CAGTGATCAC TGCTCTTGTA 
TCGATCATCA AAGTCGTTTT CAGACCCCCA GAAATCGTCC CACGTTATAT CGCAAATGAG 
GTGGATGGTG TTGTGTACCT GAAAGAACTG GAGCCTGTTA ATACTCCAAT TGCGTTTTTT 
ACCATCAGAG ATCCAGAAGG TAAATACAAG ATTAACTGCT TCTTGGATGG TGAAGGGCCA 
TTCAGGCTGG CACCCTACAA GCCGTACAAC AATGAATATT TGTTGGAGAC CACAAAGCCT 
ATGGACTATG AAGTGCAGTC CTTCTATGAA ATAGCTGTAG TGGCCTGGAA CTCAGAGGGA 
TTTCATGTCA AGAGAATCAT TAAAGTACAA CTTTTAGACG ACAATGACAA TGCCCCTGTT 
TTCCTTCAGC CCTTGATAGA ATTGACCATT GAAGAAAACA ACGCACCCAA TGCCTTTCTG 
ACAAAGCTCT ACGCTACAGA TGCTGACAGT GGAGAGATGG GACGAGTTTC CTACTTCTTG 
GGACCTGATG CTCCGTCATA CTTTTCCTTA GACAGTGTCA CAGGGATTCT GACAGTTTCT 
ACTCAGCTGG ACAGAGAAGA GAAAGAGAAG TACAGGTATA CTGTCCGAGC AGTGGACTGT 
GGGACGCCAC CCAGAGAATC AGTGGCTACA GTGGCCCTCA CTGTGTTGGA TAAAAATGAC 
AACAGTCCGA GGTTCATCAA CAAGGACTTC AGCTTTTTTG TCCCAGAAAA CTTTCCGGGG 
TATGGTGAAA TTGGAGTCAT CAGTGTAACA GATGCCGATG CTGGAAGGAA CGGATGGGTA 
GCCCTGTCCG TAATGAACCA GAGTGACATT TTTGTCATAG ACACAGGCAA GGGTATGTTG 
AGAGCTAAAG TCTCTTTAGA CAGAGAACAG CAAAGCTCTT ACACTCTGTG GGTGGAGGCT 
GTTGACGGGG GTGAGCCTGC CCTTTCCTCT ACCACAAAAA TCACAATTCT TCTTCTGGAC 
ATCAACGATA ACCCTCCTCT TGTTTTATTC CCTCAATCTA ATATGTCTTA TTTGCTGGTC 
CTACCTTCTA CTCTCCCTGG CTCACCAGTA ACAGAAGTCT ATGCGGTTGA CAAAGACACA 
GGCATGAATG CTGTCATAGC TTATAGCATC ATAGGGAGAA GAGGTCCCAG GCCTGAGTCT 
TTCAGAATTG ACCCCAAGAC TGGCAACATC ACTCTGGAGG AAGCCTTGCT TCAGACAGAT 
TATGGGTTGC ATCGCTTACT GGTGAAAGTG AGTGATCACG GATACCCTGA GCCTCTCCAC 
TCCACAGTCA TGGTGAACTT ATTTGTCAAT GACACTGTCA GTAACGAGAG CTACATTGAG 
AGCCTTTTGA GAAAGGAGCC AGAAATTAAT ATAGAAGAGA AAGAACCACA AATCTCAATA 
GAGCCAACTC ATAGGAAGGT AGAATCTATG TCCTGTATGC CCACATTGGT AGCTCTGTCA 
GTCATAAGCT TGGGCTCCAT CACACTAGTT ACAGGGATGG GAATATACAT CTGCTTACGG 
AAAGGTAAAA AGCATCACAG GGAAGATGAC AATTTGGAAG TACAGATTCC ATTGAAAGGA 
AAAATTGACT TGTGTATGAG AGAGAGAAAA CCAGTGGATA TTTCTAACAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103634.1
CDS	977..3070
Translation	MFTLDVEENENGERTPYLIVMGALDRETQDQYVSIIIAEDGGSPPLLGSATLTIGISDINDNCPLFIDSQINVTVYGNATVGTPVATVQAVDRDLGTNAQITYSYSQKVPQETKDLFHLDEVTGVIKLSSKIGGSVLQTHKLTILANGPGCIPAVITALVSIIKVVFRPPEIVPRYIANEVDGVVYLKELEPVNTPIAFFTIRDPEGKYKINCFLDGEGPFRLAPYKPYNNEYLLETTKPMDYEVQSFYEIAVVAWNSEGFHVKRIIKVQLLDDNDNAPVFLQPLIELTIEENNAPNAFLTKLYATDADSGEMGRVSYFLGPDAPSYFSLDSVTGILTVSTQLDREEKEKYRYTVRAVDCGTPPRESVATVALTVLDKNDNSPRFINKDFSFFVPENFPGYGEIGVISVTDADAGRNGWVALSVMNQSDIFVIDTGKGMLRAKVSLDREQQSSYTLWVEAVDGGEPALSSTTKITILLLDINDNPPLVLFPQSNMSYLLVLPSTLPGSPVTEVYAVDKDTGMNAVIAYSIIGRRGPRPESFRIDPKTGNITLEEALLQTDYGLHRLLVKVSDHGYPEPLHSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQISIEPTHRKVESMSCMPTLVALSVISLGSITLVTGMGIYICLRKGKKHHREDDNLEVQIPLKGKIDLCMRERKPVDISNI

Target ORF information:

RefSeq Version	XM_030247774.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protocadherin 20 (Pcdh20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247774.1

ORF Insert Sequence:

ATGTTTACCC TGGACGTGGA GGAGAATGAG AACGGGGAGC GCACACCCTA CTTGATTGTC 
ATGGGTGCTT TGGACAGAGA AACCCAGGAC CAGTATGTGA GCATCATCAT AGCTGAGGAT 
GGTGGGTCTC CACCACTGCT GGGCAGTGCC ACCCTCACCA TTGGCATAAG TGACATTAAT 
GACAATTGTC CTCTCTTTAT AGACTCACAA ATTAATGTCA CCGTTTATGG GAATGCTACA 
GTGGGCACCC CAGTTGCAAC AGTCCAGGCT GTGGATAGAG ACTTGGGGAC AAATGCACAG 
ATCACTTACA GTTACAGCCA GAAAGTTCCA CAGGAAACCA AGGATTTATT CCACCTGGAT 
GAAGTTACTG GAGTCATTAA GCTTTCCAGT AAGATTGGCG GGAGTGTCCT ACAGACACAT 
AAGCTCACCA TCCTAGCTAA TGGACCTGGC TGTATCCCTG CAGTGATCAC TGCTCTTGTA 
TCGATCATCA AAGTCGTTTT CAGACCCCCA GAAATCGTCC CACGTTATAT CGCAAATGAG 
GTGGATGGTG TTGTGTACCT GAAAGAACTG GAGCCTGTTA ATACTCCAAT TGCGTTTTTT 
ACCATCAGAG ATCCAGAAGG TAAATACAAG ATTAACTGCT TCTTGGATGG TGAAGGGCCA 
TTCAGGCTGG CACCCTACAA GCCGTACAAC AATGAATATT TGTTGGAGAC CACAAAGCCT 
ATGGACTATG AAGTGCAGTC CTTCTATGAA ATAGCTGTAG TGGCCTGGAA CTCAGAGGGA 
TTTCATGTCA AGAGAATCAT TAAAGTACAA CTTTTAGACG ACAATGACAA TGCCCCTGTT 
TTCCTTCAGC CCTTGATAGA ATTGACCATT GAAGAAAACA ACGCACCCAA TGCCTTTCTG 
ACAAAGCTCT ACGCTACAGA TGCTGACAGT GGAGAGATGG GACGAGTTTC CTACTTCTTG 
GGACCTGATG CTCCGTCATA CTTTTCCTTA GACAGTGTCA CAGGGATTCT GACAGTTTCT 
ACTCAGCTGG ACAGAGAAGA GAAAGAGAAG TACAGGTATA CTGTCCGAGC AGTGGACTGT 
GGGACGCCAC CCAGAGAATC AGTGGCTACA GTGGCCCTCA CTGTGTTGGA TAAAAATGAC 
AACAGTCCGA GGTTCATCAA CAAGGACTTC AGCTTTTTTG TCCCAGAAAA CTTTCCGGGG 
TATGGTGAAA TTGGAGTCAT CAGTGTAACA GATGCCGATG CTGGAAGGAA CGGATGGGTA 
GCCCTGTCCG TAATGAACCA GAGTGACATT TTTGTCATAG ACACAGGCAA GGGTATGTTG 
AGAGCTAAAG TCTCTTTAGA CAGAGAACAG CAAAGCTCTT ACACTCTGTG GGTGGAGGCT 
GTTGACGGGG GTGAGCCTGC CCTTTCCTCT ACCACAAAAA TCACAATTCT TCTTCTGGAC 
ATCAACGATA ACCCTCCTCT TGTTTTATTC CCTCAATCTA ATATGTCTTA TTTGCTGGTC 
CTACCTTCTA CTCTCCCTGG CTCACCAGTA ACAGAAGTCT ATGCGGTTGA CAAAGACACA 
GGCATGAATG CTGTCATAGC TTATAGCATC ATAGGGAGAA GAGGTCCCAG GCCTGAGTCT 
TTCAGAATTG ACCCCAAGAC TGGCAACATC ACTCTGGAGG AAGCCTTGCT TCAGACAGAT 
TATGGGTTGC ATCGCTTACT GGTGAAAGTG AGTGATCACG GATACCCTGA GCCTCTCCAC 
TCCACAGTCA TGGTGAACTT ATTTGTCAAT GACACTGTCA GTAACGAGAG CTACATTGAG 
AGCCTTTTGA GAAAGGAGCC AGAAATTAAT ATAGAAGAGA AAGAACCACA AATCTCAATA 
GAGCCAACTC ATAGGAAGGT AGAATCTATG TCCTGTATGC CCACATTGGT AGCTCTGTCA 
GTCATAAGCT TGGGCTCCAT CACACTAGTT ACAGGGATGG GAATATACAT CTGCTTACGG 
AAAGGTAAAA AGCATCACAG GGAAGATGAC AATTTGGAAG TACAGATTCC ATTGAAAGGA 
AAAATTGACT TGTGTATGAG AGAGAGAAAA CCAGTGGATA TTTCTAACAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular and Neural Functions of Rai1, the Causal Gene for Smith-Magenis Syndrome.
Neuron92(2)392-406(2016 Oct)
Huang WH,Guenthner CJ,Xu J,Nguyen T,Schwarz LA,Wilkinson AW,Gozani O,Chang HY,Shamloo M,Luo L

Context-specific function of the LIM homeobox 1 transcription factor in head formation of the mouse embryo.
Development (Cambridge, England)142(11)2069-79(2015 Jun)
Fossat N,Ip CK,Jones VJ,Studdert JB,Khoo PL,Lewis SL,Power M,Tourle K,Loebel DA,Kwan KM,Behringer RR,Tam PP

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Secreted TARSH regulates olfactory mitral cell dendritic complexity.
The European journal of neuroscience29(6)1083-95(2009 Mar)
Cheng TW,Gong Q

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Pcdh20 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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