Thbs1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Thbs1
Entrez Gene ID	21825
Full Name	thrombospondin 1
Synonyms	TSP-1,TSP1,Thbs-1,tbsp1
General protein information	Preferred Names thrombospondin 1 Names thrombospondin-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 E5\|2 59.34 cM
Summary	The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_011580.4	NP_035710.2	thrombospondin-1 isoform 1 precursor
NM_001313914.1	NP_001300843.1	thrombospondin-1 isoform 2 precursor

Pathways from BioSystems

Homologies

	THBS1	XP_001093770.2
Mus musculus (house mouse)	Thbs1	NP_035710.2
Gallus gallus (chicken)	THBS1	NP_001186382.1
Pan troglodytes (chimpanzee)	THBS1	XP_510294.3
Canis lupus familiaris (dog)	THBS1	XP_544610.3
Bos taurus (cattle)	THBS1	NP_776621.1
Rattus norvegicus (Norway rat)	Thbs1	NP_001013080.1
Homo sapiens (human)	THBS1	NP_003237.2
Danio rerio (zebrafish)	thbs1	XP_005160819.1
Xenopus tropicalis (tropical clawed frog)	thbs1	XP_002937245.1

Gene Ontology

The following Thbs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Thbs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21673	NM_011580.4 Latest version!	Mus musculus thrombospondin 1 (Thbs1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$692.30 $989.00
OMu30243	NM_001313914.1 Latest version!	Mus musculus thrombospondin 1 (Thbs1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Thbs1 ( NM_011580.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035710.2 Mus musculus thrombospondin 1 (Thbs1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu21673
Clone ID Related Accession (Same CDS sequence)	NM_011580.4
Accession Version	NM_011580.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	thrombospondin-1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL928957.9, AK163092.1, BC050917.1 and AL845495.9. On Sep 11, 2015 this sequence version replaced NM_011580.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050917.1, AK145202.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTCC TGCGGGGACT AGGTGTCCTG TTCCTGTTGC ATATGTGTGG AAGCAACCGC 
ATTCCAGAGT CTGGGGGAGA TAACGGTGTG TTTGACATCT TTGAACTCAT TGGAGGTGCA 
CGAAGGGGCC CCGGTCGCCG ACTGGTGAAG GGCCAAGATC TATCCAGCCC CGCCTTCCGC 
ATTGAGAATG CCAACCTGAT CCCCGCTGTG CCGGATGACA AGTTCCAAGA CCTACTGGAC 
GCTGTGTGGG CCGACAAAGG CTTCATCTTC CTGGCTTCCT TGAGGCAGAT GAAGAAGACC 
CGGGGCACAC TCCTGGCTGT GGAACGGAAA GACAACACTG GCCAGATCTT CAGTGTGGTC 
TCCAACGGCA AAGCTGGCAC CCTGGACCTG AGCCTGAGCC TGCCAGGGAA GCAACAAGTG 
GTGTCAGTGG AGGAAGCTCT CCTGGCCACT GGCCAGTGGA AGAGCATCAC GCTGTTTGTT 
CAAGAGGACC GGGCTCAACT CTACATAGAC TGTGATAAGA TGGAGAGCGC GGAGCTGGAT 
GTACCCATCC AGAGCATCTT CACCAGGGAT CTGGCCAGCG TTGCCAGGCT CCGAGTTGCA 
AAGGGAGATG TCAATGACAA TTTTCAGGGG GTGCTGCAGA ATGTGAGGTT TGTCTTTGGA 
ACCACCCCAG AAGACATTCT CAGGAACAAA GGCTGCTCCA GCTCAGCTAC CAACGTCCTT 
CTTACCCTTG ACAACAACGT GGTGAACGGT TCCAGCCCTG CTATCCGCAC CAACTACATC 
GGCCACAAAA CAAAGGACCT CCAAGCTATC TGTGGCCTCT CCTGTGATGA ACTATCCAGC 
ATGGTCCTGG AACTGAAGGG CCTGCGCACC ATCGTGACCA CTCTGCAGGA CAGCATCCGA 
AAAGTGACGG AAGAGAACAG AGAGCTGGTC AGTGAGCTGA AGCGGCCTCC CCTCTGCTTT 
CACAATGGAG TCCAGTACAA GAACAACGAG GAGTGGACTG TAGACAGTTG CACAGAGTGT 
CACTGCCAGA ACTCGGTTAC CATCTGCAAA AAGGTGTCCT GTCCCATCAT GCCCTGCTCC 
AACGCCACAG TTCCTGATGG TGAATGCTGC CCACGGTGCT GGCCCAGCGA CTCTGCTGAC 
GATGGCTGGT CTCCCTGGTC TGAGTGGACC TCCTGCTCTG CCACATGTGG CAATGGAATT 
CAGCAACGTG GTCGTTCCTG TGACAGCCTC AACAACAGAT GCGAGGGCTC TTCGGTACAG 
ACAAGGACCT GCCACATTCA GGAGTGTGAC AAAAGATTTA AACAGGATGG TGGCTGGAGT 
CACTGGTCTC CATGGTCGTC CTGTTCTGTG ACCTGTGGTG ACGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AACGGGAAGC CCTGTGAAGG TGAAGCCCGG 
GAGACCAAAG CCTGCAAGAA AGACGCCTGC CCAATTAATG GAGGCTGGGG TCCCTGGTCA 
CCATGGGACA TCTGCTCTGT CACCTGTGGA GGAGGAGTGC AGAGACGCAG CCGACTCTGT 
AACAACCCCA CACCCCAGTT TGGAGGCAAA GACTGTGTTG GCGATGTGAC AGAAAATCAA 
GTTTGCAACA AGCAGGACTG CCCAATTGAT GGATGCCTGT CCAATCCCTG CTTTGCTGGT 
GCCAAGTGTA CTAGCTACCC TGATGGTAGC TGGAAATGTG GTGCGTGTCC TCCTGGCTAC 
AGTGGAAATG GCATCCAGTG CAAAGACGTC GATGAGTGCA AAGAAGTGCC TGATGCTTGC 
TTCAATCACA ACGGAGAACA TCGGTGCAAG AACACAGATC CTGGCTACAA CTGCCTGCCC 
TGCCCACCAC GATTCACTGG CTCACAGCCC TTCGGCCGAG GTGTCGAACA TGCCATGGCC 
AACAAACAGG TGTGCAAACC GCGAAACCCC TGCACGGACG GGACGCATGA CTGCAACAAG 
AACGCTAAGT GCAACTACCT GGGTCACTAC AGTGACCCCA TGTACCGCTG TGAGTGCAAG 
CCCGGCTATG CAGGCAATGG CATCATCTGC GGAGAGGACA CAGACCTGGA CGGCTGGCCT 
AATGAAAACC TGGTGTGTGT GGCCAACGCA ACCTACCACT GCAAAAAGGA CAACTGCCCC 
AACCTTCCCA ACTCGGGGCA GGAAGACTAT GACAAGGACG GGATTGGCGA TGCCTGCGAT 
GATGACGATG ACAACGACAA GATCCCCGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ATGACTATGA CAGAGATGAT GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CTGACCAAGC AGACACAGAC AAAAACGGGG AGGGCGATGC CTGTGCTGTG 
GACATCGATG GAGATGGAAT CCTCAATGAA CGAGACAACT GCCAGTACGT TTACAACGTG 
GACCAGAGGG ACACGGACAT GGATGGGGTT GGAGATCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTGGA CTCTGACTCA GACCTCATAG GGGACACTTG TGACAACAAT 
CAGGACATCG ATGAGGATGG CCATCAGAAC AATCTGGACA ACTGTCCCTA TGTGCCTAAT 
GCCAACCAGG CCGACCATGA TAAAGATGGC AAAGGAGATG CCTGTGACCA TGACGATGAC 
AATGACGGCA TCCCTGATGA CAGAGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGCCG AGGTGACGCC TGCAAAGACG ACTTTGACCA TGACAATGTG 
CCAGATATTG ATGACATCTG TCCTGAGAAT TTTGACATCA GTGAAACCGA TTTCCGACAA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGA ACCTCCCAAA ATGACCCTAA CTGGGTTGTC 
CGCCATCAGG GCAAAGAACT TGTCCAGACT GTAAACTGTG ACCCTGGACT TGCTGTAGGT 
TATGATGAGT TTAATGCTGT GGACTTCAGC GGTACCTTCT TCATCAACAC CGAGAGAGAT 
GATGACTACG CTGGCTTTGT TTTCGGCTAC CAGTCCAGCA GCCGCTTCTA CGTTGTGATG 
TGGAAACAAG TCACCCAGTC CTACTGGGAC ACCAACCCCA CAAGGGCTCA GGGATACTCA 
GGCCTGTCTG TAAAGGTTGT GAACTCCACC ACCGGCCCTG GCGAGCACCT GCGGAATGCA 
CTGTGGCACA CAGGAAACAC CCCTGGCCAG GTGCGCACCC TGTGGCATGA CCCTCGCCAC 
ATCGGCTGGA AAGATTTCAC TGCATACAGA TGGCGTCTCA GCCACAGGCC AAAGACCGGT 
TATATCAGAG TGGTGATGTA TGAAGGAAAG AAAATCATGG CTGACTCGGG ACCCATCTAT 
GACAAAACCT ACGCCGGCGG TAGACTAGGC CTGTTCGTCT TCTCTCAGGA AATGGTGTTC 
TTCTCAGACA TGAAATACGA GTGTCGAGAT TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035710.2
CDS	229..3744
Translation	MELLRGLGVLFLLHMCGSNRIPESGGDNGVFDIFELIGGARRGPGRRLVKGQDLSSPAFRIENANLIPAVPDDKFQDLLDAVWADKGFIFLASLRQMKKTRGTLLAVERKDNTGQIFSVVSNGKAGTLDLSLSLPGKQQVVSVEEALLATGQWKSITLFVQEDRAQLYIDCDKMESAELDVPIQSIFTRDLASVARLRVAKGDVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSATNVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGLSCDELSSMVLELKGLRTIVTTLQDSIRKVTEENRELVSELKRPPLCFHNGVQYKNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSATCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQRRSRLCNNPTPQFGGKDCVGDVTENQVCNKQDCPIDGCLSNPCFAGAKCTSYPDGSWKCGACPPGYSGNGIQCKDVDECKEVPDACFNHNGEHRCKNTDPGYNCLPCPPRFTGSQPFGRGVEHAMANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDKNGEGDACAVDIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDLIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDHDNVPDIDDICPENFDISETDFRQFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGYIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDMKYECRDS

Target ORF information:

RefSeq Version	NM_011580.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin 1 (Thbs1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011580.4

ORF Insert Sequence:

ATGGAGCTCC TGCGGGGACT AGGTGTCCTG TTCCTGTTGC ATATGTGTGG AAGCAACCGC 
ATTCCAGAGT CTGGGGGAGA TAACGGTGTG TTTGACATCT TTGAACTCAT TGGAGGTGCA 
CGAAGGGGCC CCGGTCGCCG ACTGGTGAAG GGCCAAGATC TATCCAGCCC CGCCTTCCGC 
ATTGAGAATG CCAACCTGAT CCCCGCTGTG CCGGATGACA AGTTCCAAGA CCTACTGGAC 
GCTGTGTGGG CCGACAAAGG CTTCATCTTC CTGGCTTCCT TGAGGCAGAT GAAGAAGACC 
CGGGGCACAC TCCTGGCTGT GGAACGGAAA GACAACACTG GCCAGATCTT CAGTGTGGTC 
TCCAACGGCA AAGCTGGCAC CCTGGACCTG AGCCTGAGCC TGCCAGGGAA GCAACAAGTG 
GTGTCAGTGG AGGAAGCTCT CCTGGCCACT GGCCAGTGGA AGAGCATCAC GCTGTTTGTT 
CAAGAGGACC GGGCTCAACT CTACATAGAC TGTGATAAGA TGGAGAGCGC GGAGCTGGAT 
GTACCCATCC AGAGCATCTT CACCAGGGAT CTGGCCAGCG TTGCCAGGCT CCGAGTTGCA 
AAGGGAGATG TCAATGACAA TTTTCAGGGG GTGCTGCAGA ATGTGAGGTT TGTCTTTGGA 
ACCACCCCAG AAGACATTCT CAGGAACAAA GGCTGCTCCA GCTCAGCTAC CAACGTCCTT 
CTTACCCTTG ACAACAACGT GGTGAACGGT TCCAGCCCTG CTATCCGCAC CAACTACATC 
GGCCACAAAA CAAAGGACCT CCAAGCTATC TGTGGCCTCT CCTGTGATGA ACTATCCAGC 
ATGGTCCTGG AACTGAAGGG CCTGCGCACC ATCGTGACCA CTCTGCAGGA CAGCATCCGA 
AAAGTGACGG AAGAGAACAG AGAGCTGGTC AGTGAGCTGA AGCGGCCTCC CCTCTGCTTT 
CACAATGGAG TCCAGTACAA GAACAACGAG GAGTGGACTG TAGACAGTTG CACAGAGTGT 
CACTGCCAGA ACTCGGTTAC CATCTGCAAA AAGGTGTCCT GTCCCATCAT GCCCTGCTCC 
AACGCCACAG TTCCTGATGG TGAATGCTGC CCACGGTGCT GGCCCAGCGA CTCTGCTGAC 
GATGGCTGGT CTCCCTGGTC TGAGTGGACC TCCTGCTCTG CCACATGTGG CAATGGAATT 
CAGCAACGTG GTCGTTCCTG TGACAGCCTC AACAACAGAT GCGAGGGCTC TTCGGTACAG 
ACAAGGACCT GCCACATTCA GGAGTGTGAC AAAAGATTTA AACAGGATGG TGGCTGGAGT 
CACTGGTCTC CATGGTCGTC CTGTTCTGTG ACCTGTGGTG ACGGTGTGAT CACAAGGATC 
CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AACGGGAAGC CCTGTGAAGG TGAAGCCCGG 
GAGACCAAAG CCTGCAAGAA AGACGCCTGC CCAATTAATG GAGGCTGGGG TCCCTGGTCA 
CCATGGGACA TCTGCTCTGT CACCTGTGGA GGAGGAGTGC AGAGACGCAG CCGACTCTGT 
AACAACCCCA CACCCCAGTT TGGAGGCAAA GACTGTGTTG GCGATGTGAC AGAAAATCAA 
GTTTGCAACA AGCAGGACTG CCCAATTGAT GGATGCCTGT CCAATCCCTG CTTTGCTGGT 
GCCAAGTGTA CTAGCTACCC TGATGGTAGC TGGAAATGTG GTGCGTGTCC TCCTGGCTAC 
AGTGGAAATG GCATCCAGTG CAAAGACGTC GATGAGTGCA AAGAAGTGCC TGATGCTTGC 
TTCAATCACA ACGGAGAACA TCGGTGCAAG AACACAGATC CTGGCTACAA CTGCCTGCCC 
TGCCCACCAC GATTCACTGG CTCACAGCCC TTCGGCCGAG GTGTCGAACA TGCCATGGCC 
AACAAACAGG TGTGCAAACC GCGAAACCCC TGCACGGACG GGACGCATGA CTGCAACAAG 
AACGCTAAGT GCAACTACCT GGGTCACTAC AGTGACCCCA TGTACCGCTG TGAGTGCAAG 
CCCGGCTATG CAGGCAATGG CATCATCTGC GGAGAGGACA CAGACCTGGA CGGCTGGCCT 
AATGAAAACC TGGTGTGTGT GGCCAACGCA ACCTACCACT GCAAAAAGGA CAACTGCCCC 
AACCTTCCCA ACTCGGGGCA GGAAGACTAT GACAAGGACG GGATTGGCGA TGCCTGCGAT 
GATGACGATG ACAACGACAA GATCCCCGAT GACAGGGACA ACTGTCCATT CCATTACAAC 
CCAGCCCAGT ATGACTATGA CAGAGATGAT GTGGGAGACC GCTGTGACAA CTGCCCCTAC 
AACCACAACC CTGACCAAGC AGACACAGAC AAAAACGGGG AGGGCGATGC CTGTGCTGTG 
GACATCGATG GAGATGGAAT CCTCAATGAA CGAGACAACT GCCAGTACGT TTACAACGTG 
GACCAGAGGG ACACGGACAT GGATGGGGTT GGAGATCAGT GTGACAACTG CCCCCTGGAA 
CACAATCCAG ACCAGCTGGA CTCTGACTCA GACCTCATAG GGGACACTTG TGACAACAAT 
CAGGACATCG ATGAGGATGG CCATCAGAAC AATCTGGACA ACTGTCCCTA TGTGCCTAAT 
GCCAACCAGG CCGACCATGA TAAAGATGGC AAAGGAGATG CCTGTGACCA TGACGATGAC 
AATGACGGCA TCCCTGATGA CAGAGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG 
GACTCTGATG GTGATGGCCG AGGTGACGCC TGCAAAGACG ACTTTGACCA TGACAATGTG 
CCAGATATTG ATGACATCTG TCCTGAGAAT TTTGACATCA GTGAAACCGA TTTCCGACAA 
TTCCAGATGA TTCCTCTAGA TCCCAAAGGA ACCTCCCAAA ATGACCCTAA CTGGGTTGTC 
CGCCATCAGG GCAAAGAACT TGTCCAGACT GTAAACTGTG ACCCTGGACT TGCTGTAGGT 
TATGATGAGT TTAATGCTGT GGACTTCAGC GGTACCTTCT TCATCAACAC CGAGAGAGAT 
GATGACTACG CTGGCTTTGT TTTCGGCTAC CAGTCCAGCA GCCGCTTCTA CGTTGTGATG 
TGGAAACAAG TCACCCAGTC CTACTGGGAC ACCAACCCCA CAAGGGCTCA GGGATACTCA 
GGCCTGTCTG TAAAGGTTGT GAACTCCACC ACCGGCCCTG GCGAGCACCT GCGGAATGCA 
CTGTGGCACA CAGGAAACAC CCCTGGCCAG GTGCGCACCC TGTGGCATGA CCCTCGCCAC 
ATCGGCTGGA AAGATTTCAC TGCATACAGA TGGCGTCTCA GCCACAGGCC AAAGACCGGT 
TATATCAGAG TGGTGATGTA TGAAGGAAAG AAAATCATGG CTGACTCGGG ACCCATCTAT 
GACAAAACCT ACGCCGGCGG TAGACTAGGC CTGTTCGTCT TCTCTCAGGA AATGGTGTTC 
TTCTCAGACA TGAAATACGA GTGTCGAGAT TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thbs1 ( NM_001313914.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300843.1 Mus musculus thrombospondin 1 (Thbs1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu30243
Clone ID Related Accession (Same CDS sequence)	NM_001313914.1
Accession Version	NM_001313914.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	thrombospondin-1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL928957.9, AK163092.1, BC050917.1 and AL845495.9. On Sep 11, 2015 this sequence version replaced XM_006499152.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M87276.1, AK163092.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTCC TGCGGGGACT AGGTGTCCTG TTCCTGTTGC ATATGTGTGG AAGCAACCGC 
ATTCCAGAGT CTGGGGGAGA TAACGGTGTG TTTGACATCT TTGAACTCAT TGGAGGTGCA 
CGAAGGGGCC CCGGTCGCCG ACTGGTGAAG GGCCAAGATC TATCCAGCCC CGCCTTCCGC 
ATTGAGAATG CCAACCTGAT CCCCGCTGTG CCGGATGACA AGTTCCAAGA CCTACTGGAC 
GCTGTGTGGG CCGACAAAGG CTTCATCTTC CTGGCTTCCT TGAGGCAGAT GAAGAAGACC 
CGGGGCACAC TCCTGGCTGT GGAACGGAAA GACAACACTG GCCAGATCTT CAGTGTGGTC 
TCCAACGGCA AAGCTGGCAC CCTGGACCTG AGCCTGAGCC TGCCAGGGAA GCAACAAGTG 
GTGTCAGTGG AGGAAGCTCT CCTGGCCACT GGCCAGTGGA AGAGCATCAC GCTGTTTGTT 
CAAGAGGACC GGGCTCAACT CTACATAGAC TGTGATAAGA TGGAGAGCGC GGAGCTGGAT 
GTACCCATCC AGAGCATCTT CACCAGGGAT CTGGCCAGCG TTGCCAGGCT CCGAGTTGCA 
AAGGGAGATG TCAATGACAA TTTTCAGGGG GTGCTGCAGA ATGTGAGGTT TGTCTTTGGA 
ACCACCCCAG AAGACATTCT CAGGAACAAA GGCTGCTCCA GCTCTACCAA CGTCCTTCTT 
ACCCTTGACA ACAACGTGGT GAACGGTTCC AGCCCTGCTA TCCGCACCAA CTACATCGGC 
CACAAAACAA AGGACCTCCA AGCTATCTGT GGCCTCTCCT GTGATGAACT ATCCAGCATG 
GTCCTGGAAC TGAAGGGCCT GCGCACCATC GTGACCACTC TGCAGGACAG CATCCGAAAA 
GTGACGGAAG AGAACAGAGA GCTGGTCAGT GAGCTGAAGC GGCCTCCCCT CTGCTTTCAC 
AATGGAGTCC AGTACAAGAA CAACGAGGAG TGGACTGTAG ACAGTTGCAC AGAGTGTCAC 
TGCCAGAACT CGGTTACCAT CTGCAAAAAG GTGTCCTGTC CCATCATGCC CTGCTCCAAC 
GCCACAGTTC CTGATGGTGA ATGCTGCCCA CGGTGCTGGC CCAGCGACTC TGCTGACGAT 
GGCTGGTCTC CCTGGTCTGA GTGGACCTCC TGCTCTGCCA CATGTGGCAA TGGAATTCAG 
CAACGTGGTC GTTCCTGTGA CAGCCTCAAC AACAGATGCG AGGGCTCTTC GGTACAGACA 
AGGACCTGCC ACATTCAGGA GTGTGACAAA AGATTTAAAC AGGATGGTGG CTGGAGTCAC 
TGGTCTCCAT GGTCGTCCTG TTCTGTGACC TGTGGTGACG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CCCCCAGCCC CCAGATGAAC GGGAAGCCCT GTGAAGGTGA AGCCCGGGAG 
ACCAAAGCCT GCAAGAAAGA CGCCTGCCCA ATTAATGGAG GCTGGGGTCC CTGGTCACCA 
TGGGACATCT GCTCTGTCAC CTGTGGAGGA GGAGTGCAGA GACGCAGCCG ACTCTGTAAC 
AACCCCACAC CCCAGTTTGG AGGCAAAGAC TGTGTTGGCG ATGTGACAGA AAATCAAGTT 
TGCAACAAGC AGGACTGCCC AATTGATGGA TGCCTGTCCA ATCCCTGCTT TGCTGGTGCC 
AAGTGTACTA GCTACCCTGA TGGTAGCTGG AAATGTGGTG CGTGTCCTCC TGGCTACAGT 
GGAAATGGCA TCCAGTGCAA AGACGTCGAT GAGTGCAAAG AAGTGCCTGA TGCTTGCTTC 
AATCACAACG GAGAACATCG GTGCAAGAAC ACAGATCCTG GCTACAACTG CCTGCCCTGC 
CCACCACGAT TCACTGGCTC ACAGCCCTTC GGCCGAGGTG TCGAACATGC CATGGCCAAC 
AAACAGGTGT GCAAACCGCG AAACCCCTGC ACGGACGGGA CGCATGACTG CAACAAGAAC 
GCTAAGTGCA ACTACCTGGG TCACTACAGT GACCCCATGT ACCGCTGTGA GTGCAAGCCC 
GGCTATGCAG GCAATGGCAT CATCTGCGGA GAGGACACAG ACCTGGACGG CTGGCCTAAT 
GAAAACCTGG TGTGTGTGGC CAACGCAACC TACCACTGCA AAAAGGACAA CTGCCCCAAC 
CTTCCCAACT CGGGGCAGGA AGACTATGAC AAGGACGGGA TTGGCGATGC CTGCGATGAT 
GACGATGACA ACGACAAGAT CCCCGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCCCAGTATG ACTATGACAG AGATGATGTG GGAGACCGCT GTGACAACTG CCCCTACAAC 
CACAACCCTG ACCAAGCAGA CACAGACAAA AACGGGGAGG GCGATGCCTG TGCTGTGGAC 
ATCGATGGAG ATGGAATCCT CAATGAACGA GACAACTGCC AGTACGTTTA CAACGTGGAC 
CAGAGGGACA CGGACATGGA TGGGGTTGGA GATCAGTGTG ACAACTGCCC CCTGGAACAC 
AATCCAGACC AGCTGGACTC TGACTCAGAC CTCATAGGGG ACACTTGTGA CAACAATCAG 
GACATCGATG AGGATGGCCA TCAGAACAAT CTGGACAACT GTCCCTATGT GCCTAATGCC 
AACCAGGCCG ACCATGATAA AGATGGCAAA GGAGATGCCT GTGACCATGA CGATGACAAT 
GACGGCATCC CTGATGACAG AGACAACTGC AGGCTGGTGC CCAATCCTGA CCAGAAGGAC 
TCTGATGGTG ATGGCCGAGG TGACGCCTGC AAAGACGACT TTGACCATGA CAATGTGCCA 
GATATTGATG ACATCTGTCC TGAGAATTTT GACATCAGTG AAACCGATTT CCGACAATTC 
CAGATGATTC CTCTAGATCC CAAAGGAACC TCCCAAAATG ACCCTAACTG GGTTGTCCGC 
CATCAGGGCA AAGAACTTGT CCAGACTGTA AACTGTGACC CTGGACTTGC TGTAGGTTAT 
GATGAGTTTA ATGCTGTGGA CTTCAGCGGT ACCTTCTTCA TCAACACCGA GAGAGATGAT 
GACTACGCTG GCTTTGTTTT CGGCTACCAG TCCAGCAGCC GCTTCTACGT TGTGATGTGG 
AAACAAGTCA CCCAGTCCTA CTGGGACACC AACCCCACAA GGGCTCAGGG ATACTCAGGC 
CTGTCTGTAA AGGTTGTGAA CTCCACCACC GGCCCTGGCG AGCACCTGCG GAATGCACTG 
TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACCCTGT GGCATGACCC TCGCCACATC 
GGCTGGAAAG ATTTCACTGC ATACAGATGG CGTCTCAGCC ACAGGCCAAA GACCGGTTAT 
ATCAGAGTGG TGATGTATGA AGGAAAGAAA ATCATGGCTG ACTCGGGACC CATCTATGAC 
AAAACCTACG CCGGCGGTAG ACTAGGCCTG TTCGTCTTCT CTCAGGAAAT GGTGTTCTTC 
TCAGACATGA AATACGAGTG TCGAGATTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300843.1
CDS	229..3741
Translation	MELLRGLGVLFLLHMCGSNRIPESGGDNGVFDIFELIGGARRGPGRRLVKGQDLSSPAFRIENANLIPAVPDDKFQDLLDAVWADKGFIFLASLRQMKKTRGTLLAVERKDNTGQIFSVVSNGKAGTLDLSLSLPGKQQVVSVEEALLATGQWKSITLFVQEDRAQLYIDCDKMESAELDVPIQSIFTRDLASVARLRVAKGDVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTNVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGLSCDELSSMVLELKGLRTIVTTLQDSIRKVTEENRELVSELKRPPLCFHNGVQYKNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSATCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQRRSRLCNNPTPQFGGKDCVGDVTENQVCNKQDCPIDGCLSNPCFAGAKCTSYPDGSWKCGACPPGYSGNGIQCKDVDECKEVPDACFNHNGEHRCKNTDPGYNCLPCPPRFTGSQPFGRGVEHAMANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDKNGEGDACAVDIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDLIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDHDNVPDIDDICPENFDISETDFRQFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGYIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDMKYECRDS

Target ORF information:

RefSeq Version	NM_001313914.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin 1 (Thbs1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313914.1

ORF Insert Sequence:

ATGGAGCTCC TGCGGGGACT AGGTGTCCTG TTCCTGTTGC ATATGTGTGG AAGCAACCGC 
ATTCCAGAGT CTGGGGGAGA TAACGGTGTG TTTGACATCT TTGAACTCAT TGGAGGTGCA 
CGAAGGGGCC CCGGTCGCCG ACTGGTGAAG GGCCAAGATC TATCCAGCCC CGCCTTCCGC 
ATTGAGAATG CCAACCTGAT CCCCGCTGTG CCGGATGACA AGTTCCAAGA CCTACTGGAC 
GCTGTGTGGG CCGACAAAGG CTTCATCTTC CTGGCTTCCT TGAGGCAGAT GAAGAAGACC 
CGGGGCACAC TCCTGGCTGT GGAACGGAAA GACAACACTG GCCAGATCTT CAGTGTGGTC 
TCCAACGGCA AAGCTGGCAC CCTGGACCTG AGCCTGAGCC TGCCAGGGAA GCAACAAGTG 
GTGTCAGTGG AGGAAGCTCT CCTGGCCACT GGCCAGTGGA AGAGCATCAC GCTGTTTGTT 
CAAGAGGACC GGGCTCAACT CTACATAGAC TGTGATAAGA TGGAGAGCGC GGAGCTGGAT 
GTACCCATCC AGAGCATCTT CACCAGGGAT CTGGCCAGCG TTGCCAGGCT CCGAGTTGCA 
AAGGGAGATG TCAATGACAA TTTTCAGGGG GTGCTGCAGA ATGTGAGGTT TGTCTTTGGA 
ACCACCCCAG AAGACATTCT CAGGAACAAA GGCTGCTCCA GCTCTACCAA CGTCCTTCTT 
ACCCTTGACA ACAACGTGGT GAACGGTTCC AGCCCTGCTA TCCGCACCAA CTACATCGGC 
CACAAAACAA AGGACCTCCA AGCTATCTGT GGCCTCTCCT GTGATGAACT ATCCAGCATG 
GTCCTGGAAC TGAAGGGCCT GCGCACCATC GTGACCACTC TGCAGGACAG CATCCGAAAA 
GTGACGGAAG AGAACAGAGA GCTGGTCAGT GAGCTGAAGC GGCCTCCCCT CTGCTTTCAC 
AATGGAGTCC AGTACAAGAA CAACGAGGAG TGGACTGTAG ACAGTTGCAC AGAGTGTCAC 
TGCCAGAACT CGGTTACCAT CTGCAAAAAG GTGTCCTGTC CCATCATGCC CTGCTCCAAC 
GCCACAGTTC CTGATGGTGA ATGCTGCCCA CGGTGCTGGC CCAGCGACTC TGCTGACGAT 
GGCTGGTCTC CCTGGTCTGA GTGGACCTCC TGCTCTGCCA CATGTGGCAA TGGAATTCAG 
CAACGTGGTC GTTCCTGTGA CAGCCTCAAC AACAGATGCG AGGGCTCTTC GGTACAGACA 
AGGACCTGCC ACATTCAGGA GTGTGACAAA AGATTTAAAC AGGATGGTGG CTGGAGTCAC 
TGGTCTCCAT GGTCGTCCTG TTCTGTGACC TGTGGTGACG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CCCCCAGCCC CCAGATGAAC GGGAAGCCCT GTGAAGGTGA AGCCCGGGAG 
ACCAAAGCCT GCAAGAAAGA CGCCTGCCCA ATTAATGGAG GCTGGGGTCC CTGGTCACCA 
TGGGACATCT GCTCTGTCAC CTGTGGAGGA GGAGTGCAGA GACGCAGCCG ACTCTGTAAC 
AACCCCACAC CCCAGTTTGG AGGCAAAGAC TGTGTTGGCG ATGTGACAGA AAATCAAGTT 
TGCAACAAGC AGGACTGCCC AATTGATGGA TGCCTGTCCA ATCCCTGCTT TGCTGGTGCC 
AAGTGTACTA GCTACCCTGA TGGTAGCTGG AAATGTGGTG CGTGTCCTCC TGGCTACAGT 
GGAAATGGCA TCCAGTGCAA AGACGTCGAT GAGTGCAAAG AAGTGCCTGA TGCTTGCTTC 
AATCACAACG GAGAACATCG GTGCAAGAAC ACAGATCCTG GCTACAACTG CCTGCCCTGC 
CCACCACGAT TCACTGGCTC ACAGCCCTTC GGCCGAGGTG TCGAACATGC CATGGCCAAC 
AAACAGGTGT GCAAACCGCG AAACCCCTGC ACGGACGGGA CGCATGACTG CAACAAGAAC 
GCTAAGTGCA ACTACCTGGG TCACTACAGT GACCCCATGT ACCGCTGTGA GTGCAAGCCC 
GGCTATGCAG GCAATGGCAT CATCTGCGGA GAGGACACAG ACCTGGACGG CTGGCCTAAT 
GAAAACCTGG TGTGTGTGGC CAACGCAACC TACCACTGCA AAAAGGACAA CTGCCCCAAC 
CTTCCCAACT CGGGGCAGGA AGACTATGAC AAGGACGGGA TTGGCGATGC CTGCGATGAT 
GACGATGACA ACGACAAGAT CCCCGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCCCAGTATG ACTATGACAG AGATGATGTG GGAGACCGCT GTGACAACTG CCCCTACAAC 
CACAACCCTG ACCAAGCAGA CACAGACAAA AACGGGGAGG GCGATGCCTG TGCTGTGGAC 
ATCGATGGAG ATGGAATCCT CAATGAACGA GACAACTGCC AGTACGTTTA CAACGTGGAC 
CAGAGGGACA CGGACATGGA TGGGGTTGGA GATCAGTGTG ACAACTGCCC CCTGGAACAC 
AATCCAGACC AGCTGGACTC TGACTCAGAC CTCATAGGGG ACACTTGTGA CAACAATCAG 
GACATCGATG AGGATGGCCA TCAGAACAAT CTGGACAACT GTCCCTATGT GCCTAATGCC 
AACCAGGCCG ACCATGATAA AGATGGCAAA GGAGATGCCT GTGACCATGA CGATGACAAT 
GACGGCATCC CTGATGACAG AGACAACTGC AGGCTGGTGC CCAATCCTGA CCAGAAGGAC 
TCTGATGGTG ATGGCCGAGG TGACGCCTGC AAAGACGACT TTGACCATGA CAATGTGCCA 
GATATTGATG ACATCTGTCC TGAGAATTTT GACATCAGTG AAACCGATTT CCGACAATTC 
CAGATGATTC CTCTAGATCC CAAAGGAACC TCCCAAAATG ACCCTAACTG GGTTGTCCGC 
CATCAGGGCA AAGAACTTGT CCAGACTGTA AACTGTGACC CTGGACTTGC TGTAGGTTAT 
GATGAGTTTA ATGCTGTGGA CTTCAGCGGT ACCTTCTTCA TCAACACCGA GAGAGATGAT 
GACTACGCTG GCTTTGTTTT CGGCTACCAG TCCAGCAGCC GCTTCTACGT TGTGATGTGG 
AAACAAGTCA CCCAGTCCTA CTGGGACACC AACCCCACAA GGGCTCAGGG ATACTCAGGC 
CTGTCTGTAA AGGTTGTGAA CTCCACCACC GGCCCTGGCG AGCACCTGCG GAATGCACTG 
TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACCCTGT GGCATGACCC TCGCCACATC 
GGCTGGAAAG ATTTCACTGC ATACAGATGG CGTCTCAGCC ACAGGCCAAA GACCGGTTAT 
ATCAGAGTGG TGATGTATGA AGGAAAGAAA ATCATGGCTG ACTCGGGACC CATCTATGAC 
AAAACCTACG CCGGCGGTAG ACTAGGCCTG TTCGTCTTCT CTCAGGAAAT GGTGTTCTTC 
TCAGACATGA AATACGAGTG TCGAGATTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Thbs1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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